# Patent

## Title
Fused azole-pyrimidine derivatives

## Abstract

The present invention relates to hovel fused azolepyrimidine derivatives, processes for preparing them and pharmaceutical preparations containing them. The fused azolepyrimidine derivatives of the present invention exhibit enhanced potency for phosphotidylinositol-3-kinase (PI3K) inhibition, especially for PI3K-γ inhibition and can be used for the prophylaxis and treatment of diseases associated with PI3K and particularly with PI3K-γ activity. More specifically, the azole derivatives of the present invention are useful for treatment and prophylaxis of diseases as follows: inflammatory and immunoregulatory disorders, such as asthma, atopic dermatitis, rhinitis, allergic diseases, chronic obstructive pulmonary disease (COPD), septic shock, joint diseases, autoixnmune pathologies such as rheumatoid arthritis, and Graves' disease, cancer, myocardial contractility disorders, heart failure, thromboembolism, ischemia, and atherosclerosis. The compounds of the present invention are also useful for pulmonary hypertension, renal failure, cardiac hypertrophy, as well as neurodegenerative disorders such as Parkinson's disease, Alzheimer's disease, diabetes and focal ischemia, since the diseases also relate to PI3K activity in a human or animal subject.

## Background
RELATED APPLICATIONS/PATENTS AND INCORPORATION BY REFERENCE
This application is a National Stage Application filed under 35 U.S.C. § 371 based on International Application No. PCT/EP2003/010377, filed Sep. 18, 2003, which claims priority to European Patent Application Number 02021861.6, filed Sep. 30, 2002, the entire contents each of which are incorporated herein by reference.
The foregoing applications, and all documents cited therein and all documents cited or referenced therein, and all documents cited or referenced herein, including any U.S. or foreign patents or published patent applications, International patent applications, as well as, any non-patent literature references and any manufacturer's instructions, are hereby expressly incorporated herein by reference.
DETAILED DESCRIPTION OF INVENTION
1. Technical Field
The present invention relates to novel fused azolepyrimidine derivatives, processes for preparing them and pharmaceutical preparations containing them. The fused azolepyrimidine derivatives of the present invention exhibit enhanced potency for phosphotidylinositol-3-kinase (PI3K) inhibition, especially for PI3K-γ inhibition and can be used for the prophylaxis and treatment of diseases associated with PI3K and particularly with PI3K-γ activity.
More specifically, the fused azolepyrimidine derivatives of the present invention are useful for treatment and prophylaxis of diseases as follows: inflammatory and immunoregulatory disorders, such as asthma, atopic dermatitis, rhinitis, allergic diseases, chronic obstructive pulmonary disease (COPD), septic shock, joint diseases, autoimmune pathologies such as rheumatoid arthritis, and Graves' disease, cancer, myocardial contractility disorders, heart failure, thromboembolism, ischemia, and atherosclerosis.
The compounds of the present invention are also useful for pulmonary hypertension, renal failure, cardiac hypertrophy, as well as neurodegenerative disorders such as Parkinson's disease, Alzheimer's disease, diabetes and focal ischemia, since the diseases also relate to PI3K activity in a human or animal subject.
2. Background Art
Signal transduction pathways originating from chemoattractant receptors are considered to be important targets in controlling leukocyte motility in inflammatory diseases. Leukocyte trafficking is controlled by chemoattractant factors that activate heterotrimeric G-protein coupled receptors (GPCRs) and thereby trigger a complex variety of downstream intracellular events. Signal transduction at one of the pathways, that results in mobilization of intracellular free Ca2+, cytoskeletal reorganisation, and directional movement depends on lipid-derived second messengers produced by phosphoinositide 3-kinase (PI3K) activity [1,2].
PI3K phosphorylates the D3-hydroxyl position of the membrane phospholipid phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P2) to yield phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3). Based on substrate specificity and protein structure, the PI3K family comprises three classes [4-6]. Of particular interest in leukocyte migration are class I PI3Ks, which are all involved in receptor-induced inflammatory cellular responses and are further divided into the subclasses IA (p110α, β, δ) and IB (p110γ).
Class IA enzymes (p110α, β, δ) associate with a p85 adapter subunit, which contains two SH2 domains, to form a heterodimeric complex. This complex is able to recognize phosphotyrosine YxxM motifs, resulting in association with receptor tyrosine kinases and subsequent activation of the enzyme through receptor tyrosine kinases [1, 2]. The class IA subtypes are considered to be associated with cell proliferation and carcinogenesis. The IA subtypes bind to activated ras oncogene, which is found in many cancers, to express their enzyme activity. It has also found that both p110α and β play important roles in human cancer growth [3].
Class IB (p110γ) enzyme, whose expression is largely confined to leukocytes, is activated by the G protein βγ complex, and functions downstream of seven transmembrane chemoattractant receptors [7-9]. The p101 adapter protein, which bears no resemblance to any other known protein, is essential for the G protein βγ responsiveness of the p110γ (PI3Kγ). [10-12].
Recent studies in mice lacking functional PI3Kγ (PI3Kγ−/− mice), which were viable, fertile, and displayed a normal life span in a conventional mouse facility, have revealed that neutrophils are unable to produce PtdIns(3,4,5)P3 when stimulated with GPCR agonists such as fMLP, C5a or IL-8. This demonstrates that PI3Kγ is the sole PI3K that is coupled to these GPCRs in these cells [13-16]. Moreover, PtdIns(3,4,5)P3-dependent activation of protein kinase B (PKB) was also absent in those neutrophils, while PKB could still be activated by GM-CSF or IgG/C3b-coated zymosan via either p110α, β or δ. At the same time, G-protein-mediated responses such as PLCβ activation were intact. PI3Kγ−/− mice showed impaired thymocyte development and increases in neutrophil, monocyte, and eosinophil populations [14]. Furthermore, neutrophils and macrophages isolated from PI3Kγ−/− mice exhibited severe defects in migration and respiratory burst in response to GPCR agonists and chemotactic agents [14,16]. Expression of PI3Kγ was also examined in transgenic mice expressing green fluorescence protein (GFP) under the control of the endogenous PI3Kγ promoter. GFP was detected in spleen and bone marrow cells, and neutrophils, suggesting that the expression of PI3Kγ is restricted to hematopoietic cells [15]. Collectively, the class IB phosphoinositide 3-kinase PI3Kγ seems to be pivotal in the control of leukocyte trafficking and accordingly the development of isotype-selective inhibitors of PI3Kγ should be an attractive anti-inflammatory strategy.
Hypertrophic responses can be initiated by PI3K signaling pathways. Currently new research was published which identify a function for PTEN-PI3Kγ pathway in the modulation of heart muscle contractility. Whereas PI3Kα mediates the alteration in cell size seen during heart hyperthrophy up to heart failure, PI3Kγ acts as a negative regulator of cardiac contractility.
PTEN is a dual-specificity protein phosphatase recently implicated as a phospho-inositide phosphatase in cellular growth signaling. The tumor suppressor PTEN is shown to dephosphorylate phosphatidylinositol 3,4,5-triphosphate (PIP3) which is an important second messenger generated specifically by the actions of PI3K. The PTEN reduces the levels of PIP3 within the cells and antagonizes PI3K mediated cellular signaling. It is also reported that expression of dominant-negative PTEN in rat cardiomyocytes in tissue culture results in hypertrophy.
PI3Kγ modulates baseline cAMP levels and controls contractility in cells. This study also indicates that alterations in baseline cAMP level contribute to the increased contractility in mutant mice [17].
Therefore, this research result shows that PI3Kγ is involved in myocardial contractility and therefore the inhibitors would be potential treatments of congestive heart failure, ischemia, pulmonary hypertension, renal failure, cardiac hypertrophy, atherosclerosis, thromboembolism, and diabetes.
A inhibitor of PI3K, which is expected to block signaltranduction from GPCR and the activation of various immune cells, should have a broad anti-inflammatory profile with potential for the treatment of inflammatory and immunoregulatory disorders, [2] including asthma, atopic dermatitis, rhinitis, allergic diseases, chronic obstructive pulmonary disease (COPD), septic shock, joint diseases, autoimmune pathologies such as rheumatoid arthritis, and Graves' disease, diabetes, cancer, myocardial contractility disorders, thromboembolism [18], and atherosclerosis.
Some PI3-kinase inhibitors has been identified: wortmannin, originally isolated as a fungal toxin from Penicillium wortmannii [19], the closely related but less well characterized demethoxyviridin and LY294002, a morpholino derivative of the broad-spectrum kinase inhibitor quercetin [20].
U.S. Pat. No. 3,644,354 discloses 5-substituted 2,3,dihydroimidazo[1,2-c]quinazolines represented by the general formula:




wherein R and R0 is independently, hydrogen, lower alkyl, lower alkenyl; R′ and R′′ are independently, hydrogen, halogen, lower alkyl, lower alkoxy or





as a hypotensive agents and coronary dilators

However, none of the references discloses fused azolepyrimidine such as, but not limited to, azole-quinazoline, azole-pyridopyrimidine, azole-pyrimidopyrimidine, azole-pyrimidopyridazine, azole-pyrimidotriazine, azole-pteridine, azole-pyrimidotetrazine and other derivatives having acylated amine or —CR5R6—C(O)— (R5is hydrogen or C1-6 alkyl and R6 is halogen, hydrogen, or C1-6 alkyl) linker at the 5 or 6 position of the fused azolepyrimidine also having PI3K inhibitory activity.
The development of a compound which is useful for treatment and prophylaxis of inflammatory, cancer and/or myocardial contractility disorders associated with PI3K activity has been still desired.
SUMMARY OF THE INVENTION
As a result of extensive studies on chemical modification of the fused azolepyrimidine derivatives, the present inventors have found that the compounds of novel chemical structure related to the present invention have PI3K inhibitory activity and particularly have PI3K-γ inhibitory activity. The present invention has been accomplished based on these findings.


This invention is to provide novel fused azolepyrimidine derivatives of the formula (I) their tautomeric and stereoisomeric forms, and salts thereof.




wherein

    X represents CR5R6 or NH;
    Y1 represents CR3 or N;
    Chemical bond between Y2═Y3 represents a single bond or double bond,
    with the proviso that when the Y2═Y3 represents a double bond,
    Y2 and Y3 independently represent CR4 or N, and
    when Y2═Y3 represents a single bond, Y2 and Y3 independently represent CR3R4 or NR4;
    Z1, Z2, Z3 and Z4 independently represent CH, CR2 or N;
    R1 represents aryl optionally having 1 to 3 substituents selected from R11, C3-8 cycloalkyl optionally having 1 to 3 substituents selected from R11,
    
        C1-6 alkyl optionally substituted by aryl, heteroaryl, C1-6 alkoxyaryl, aryloxy, heteroaryloxy or one or more halogen,
        C1-6 alkoxy optionally substituted by carboxy, aryl, heteroaryl, C1-6 alkoxyaryl, aryloxy, heteroaryloxy or one or more halogen,
        or
        a 3 to 15 membered mono- or bi-cyclic heterocyclic ring that is saturated or unsaturated, and contains 1 to 3 heteroatoms selected from the group consisting of N, O and S, and optionally having 1 to 3 substituents selected from R11 
        wherein
        R11 represents halogen, nitro, hydroxy, cyano, carboxy, amino, N—(C1-6alkyl)amino, N-(hydroxyC1-6alkyl)amino, N,N-di(C1-6alkyl)amino, N—(C1-6acyl)amino, N-(formyl)-N—(C1-6alkyl)amino, N—(C1-6alkanesulfonyl)amino, N-(carboxyC1-6alkyl)-N—(C1-6alkyl)amino, N—(C1-6alkoxycarbonyl)amino, N-[N,N-di(C1-6alkyl)amino methylene]amino, N—[N,N-di(C1-6alkyl)amino (C1-6alkyl)methylene]amino, N—[N,N-di(C1-6alkyl)amino C2-6alkenyl]amino, aminocarbonyl, N—(C1-6alkyl)aminocarbonyl, N,N-di(C1-6alkyl)aminocarbonyl, C3-8cycloalkyl, C1-6 alkylthio, C1-6alkanesulfonyl, sulfamoyl, C1-6alkoxycarbonyl,
        N-arylamino wherein said aryl moiety is optionally having 1 to 3 substituents selected from R101, N-(aryl C1-6alkyl)amino wherein said aryl moiety is optionally having 1 to 3 substituents selected from R101, aryl C1-6alkoxy-carbonyl wherein said aryl moiety is optionally having 1 to 3 substituents selected from R101,
        C1-6alkyl optionally substituted by mono-, di- or tri-halogen, amino, N—(C1-6alkyl)amino or N,N-di(C1-6alkyl)amino,
        C1-6alkoxy optionally substituted by
        mono-, di- or tri-halogen, N—(C1-6alkyl)sulfonamide, or N-(aryl)sulfonamide,
        or
        a 5 to 7 membered saturated or unsaturated ring having 1 to 3 heteroatoms selected from the group consisting of O, S and N, and optionally having 1 to 3 substituents selected from R101 
        wherein
        R101 represents halogen, carboxy, amino, N—(C1-6 alkyl)amino, N,N-di(C1-6alkyl)amino, aminocarbonyl, N—(C1-6alkyl)aminocarbonyl, N,N-di(C1-6alkyl)aminocarbonyl, pyridyl,
        C1-6 alkyl optionally substituted by cyano or mono- di- or tri-halogen,
        or
        C1-6alkoxy optionally substituted by cyano, carboxy, amino, N—(C1-6 alkyl)amino, N,N-di(C1-6alkyl)amino, aminocarbonyl, N—(C1-6alkyl)aminocarbonyl, N,N-di(C1-6alkyl)aminocarbonyl or mono-, di- or tri-halogen;
    
    
    R2 represents hydroxy, halogen, nitro, cyano, amino, N—(C1-6alkyl)amino, N,N-di(C1-6alkyl)amino, N-(hydroxyC1-6alkyl)amino, N-(hydroxyC1-6alkyl)-N—(C1-6alkyl)amino, C1-6 acyloxy, aminoC1-6 acyloxy, C2-6alkenyl, aryl, a 5-7 membered saturated or unsaturated heterocyclic ring having 1 to 3 heteroatoms selected from the group consisting O, S and N, and optionally substituted by
    
        hydroxy, C1-6 alkyl, C1-6 alkoxy, oxo, amino, amino C1-6alkyl, N—(C1-6alkyl)amino, N,N-di(C1-6alkyl)amino, N—(C1-6 acyl)amino, N—(C1-6alkyl)carbonylamino, phenyl, phenyl C1-6 alkyl, carboxy, C1-6alkoxycarbonyl, aminocarbonyl, N—(C1-6alkyl)aminocarbonyl, or N,N-di(C1-6alkyl)amino, —C(O)—R20 
        wherein
        R20 represents C1-6 alkyl, C1-6 alkoxy, amino, N—(C1-6alkyl)amino, N,N-di(C1-6alkyl)amino, N—(C1-6 acyl)amino, or a 5-7 membered saturated or unsaturated heterocyclic ring having 1 to 3 heteroatoms selected from the group consisting O, S and N, and optionally substituted by C1-6 alkyl, C1-6 alkoxy, oxo, amino, N—(C1-6alkyl)amino, N,N-di(C1-6alkyl)amino, N—(C1-6 acyl)amino, phenyl, or benzyl,
        C1-6 alkyl optionally substituted by R21 
        or
        C1-6 alkoxy optionally substituted by R21 
        wherein
        R21 represents cyano, mono-, di or tri-halogen, hydroxy, amino, N—(C1-6alkyl)amino, N,N-di(C1-6alkyl)amino, N-(hydroxyC1-6 alkyl) amino, N-(halophenylC1-6 alkyl) amino, amino C2-6 alkylenyl, C1-6 alkoxy, hydroxyC1-6 alkoxy, —C(O)—R201, —NHC(O)—R201, C3-8cycloalkyl, isoindolino, phthalimidyl, 2-oxo-1,3-oxazolidinyl, aryl or a 5 or 6 membered saturated or unsaturated heterocyclic ring having 1 to 4 heteroatoms selected from the group consisting O, S and N optionally substituted by
        
            hydroxy, C1-6 alkyl, C1-6 alkoxy, C1-6 alkoxycarbonyl, hydroxyC1-6 alkoxy, oxo, amino, aminoC1-6alkyl, N—(C1-6alkyl)amino, N,N-di(C1-6alkyl)amino, N—(C1-6 acyl)amino, or benzyl,
            wherein
            R201 represents hydroxy, amino, N—(C1-6alkyl)amino, N,N-di(C1-6alkyl)amino, N-(halophenylC1-6 alkyl) amino, C1-6alkyl, aminoC1-6 alkyl, aminoC2-6 alkylenyl, C1-6 alkoxy, a 5 or 6 membered saturated or unsaturated heterocyclic ring having 1 to 4 heteroatoms selected from the group consisting O, S and N optionally substituted by hydroxy, C1-6 alkyl, C1-6 alkoxy, C1-6 alkoxycarbonyl, hydroxyC1-6 alkoxy, oxo, amino, N—(C1-6alkyl)amino, N,N-di(C1-6alkyl)amino, N—(C1-6 acyl)amino or benzyl;
        
        
        R3 represents hydrogen, halogen, aminocarbonyl, or C1-6 alkyl optionally substituted by aryl C1-6 alkoxy or mono-, di- or tri-halogen;
        R4 represents hydrogen or C1-6 alkyl;
        R5 represents hydrogen or C1-6 alkyl; and
        R6 represents halogen, hydrogen or C1-6 alkyl.
    
    


The compounds of the present invention show PI3K inhibitory activity and PI3K-γ inhibitory activity. They are, therefore, suitable for the production of medicament or medical composition, which may be useful for treatment and prophylaxis of PI3K and/or PI3K-γ related diseases for example, inflammatory and immunoregulatory disorders, such as asthma, atopic dermatitis, rhinitis, allergic diseases, chronic obstructive pulmonary disease (COPD), septic shock, joint diseases, autoimmune pathologies such as rheumatoid arthritis, and Graves' disease, myocardial contractility disorders, heart failure, thromboembolism, ischemia, cardiac hypertrophy, atherosclerosis and cancer such as skin cancer, bladder cancer, breast cancer, uterus cancer, ovary cancer, prostate cancer, lung cancer, colon cancer, pancreas cancer, renal cancer, gastric cancer, brain tumor, leukemia, etc.
The compounds of the present invention are also useful for treatment of pulmonary hypertension, renal failure, Huntington's chorea and cardiac hypertrophy, as well as neurodegenerative disorders such as Parkinson's disease, Alzheimer's disease, diabetes and focal ischemia, since the diseases also relate to PI3K activity in a human or animal subject.
This invention is also to provide a method for treating or preventing a disorder or disease associated with PI3K activity, especially with PI3K-γ activity, in a human or animal subject, comprising administering to said subject a therapeutically effective amount of the fused azolepyrimidine derivatives shown in the formula (I), its tautomeric or stereoisomeric form, or a physiologically acceptable salt thereof.
Further this invention is to provide a use of the fused azolepyrimidine derivatives shown in the formula (I), its tautomeric or stereoisomeric form, or a physiologically acceptable salt thereof in the preparation of a medicament.
In one embodiment, the present invention provides the fused azolepyrimidine derivative of the formula (I), its tautomeric or stereoisomeric form, or a salt thereof; wherein

    X represents CR5R6 or NH;
    Y1 represents CR3 or N;
    Chemical bond between Y2═Y3 represents a single bond or double bond, with the proviso that when the Y2═Y3 represents a double bond,
    Y2 and Y3 independently represent CR4 or N, and
    when Y2═Y3 represents a single bond, Y2 and Y3 independently represent CR3R4 or NR4;
    Z1, Z2, Z3 and Z4 independently represent CH, CR2 or N;
    R1 represents
    C1-6 alkyl optionally substituted by mono-, di- or tri-halogen, phenyl, methoxyphenyl, phenoxy, or thienyl,
    C1-6 alkoxy optionally substituted by mono-, di- or tri-halogen, phenyl, methoxy-phenyl, phenoxy, or thienyl,
    or
    one of the following carbocyclic and heterocyclic rings selected from the group consisting of cyclopropyl, cyclohexyl, piperidinyl, piperazinyl, pyrrolyl, pyrazolyl, furyl, thienyl, thiazolyl, isothiazolyl, oxazolyl, isoxazolyl, imidazolyl, isoimidazolyl, pyrazolyl, 1,2,3-thiadiazolyl, 1,2,4-thiadiazolyl, 1,2,5-thiadiazolyl, 1,3,4-thiadiazolyl, 1,2,3-oxadiazolyl, 1,2,4-oxadiazolyl, 1,2,5-oxadiazolyl, 1,3,4-oxadiazolyl, 1,2,3-triazole, 1,2,4-triazole, 1,2,5-triazole, 1,3,4-triazole, phenyl, pyridyl, pyrazinyl, pyrimidinyl, pyridazinyl, 1-benzothiophenyl, benzothiazolyl, benzimidazolyl, 3H-imidazo[4,5-b]pyridinyl, benzotriazolyl, indolyl, indazolyl, imidazo[1,2-a]pyridinyl, quinolinyl, and 1,8-naphthyridinyl,
    wherein
    said carbocyclic and heterocyclic rings optionally substituted with 1 to 3 substituents selected from the group consisting of hydroxy, halogen, nitro, cyano, carboxy, amino, N—(C1-6alkyl)amino, N,N-di(C1-6alkyl)amino, N—(C1-6acyl)amino, N—(C1-6alkoxycarbonyl)amino, N-(formyl)-N—(C1-6alkyl)amino, N[N,N-di(C1-6alkyl)amino methylene]amino, N[N,N-di(C1-6alkyl)amino (C1-6alkylene)methylene]amino, N—[N,N-di(C1-6alkyl)amino C2-6alkenyl]amino, C1-6 alkylthio, C1-6alkanesulfonyl, sulfamoyl, C1-6alkoxy, C1-6alkoxycarbonyl, pyrrolyl, imidazolyl, pyrazolyl, pyrrolidinyl, pyridyl, phenyl C1-6alkoxycarbonyl,
    thiazolyl optionally substituted by pyridyl,
    piperazinyl optionally substituted by C1-6 alkyl or C1-6alkoxy and
    C1-6alkyl optionally substituted by mono-, di- or tri-halogen;
    R2 represents hydroxy, halogen, nitro, cyano, carboxy, amino, N—(C1-6alkyl)amino, N-(hydroxy C1-6alkyl)amino, N,N-di(C1-6alkyl)amino, N-(hydroxy C1-6alkyl)-N—(C1-6alkyl)amino, C2-6alkenyl, C1-6alkoxycarbonyl, aminocarbonyl, C1-6acyloxy, aminoC1-6 acyloxy, furyl, morpholino, phenyl, piperidino, aryl,
    pyrrolidinyl optionally substituted by C1-6acylamino, piperidino optionally substituted by hydroxy, C1-6 alkyl, carboxy, aminocarbonyl, N—(C1-6alkyl)aminocarbonyl, or N,N-di(C1-6alkyl)aminocarbonyl,
    piperazinyl optionally substituted by C1-6 alkyl,
    C1-6 alkyl optionally substituted by cyano, mono-, di- or tri-halogen, hydroxy, amino, N—(C1-6alkyl)amino, N-(hydroxy C1-6alkyl)amino, N,N-di(C1-6alkyl)amino, C3-6 cycloalkyl, tetrazolyl, tetrahydropyranyl, morpholino, phthalimidyl, 2-oxo-1,3oxazolidinyl, phenyl,
    —C(O)—R201,
    pyrrolidinyl optionally substituted by C1-6acylamino, piperidino optionally substituted by hydroxy, C1-6 alkyl, carboxy, aminocarbonyl, N—(C1-6alkyl)aminocarbonyl, or N,N-di(C1-6alkyl)aminocarbonyl,
    or
    piperazinyl optionally substituted by C1-6 alkyl,
    wherein
    R201 represents hydroxy, amino, N—(C1-6alkyl)amino, N,N-di(C1-6alkyl)amino, N-halobenzyl)amino, C1-6alkyl, C1-6 alkoxy, tetrazolyl, tetrahydropyranyl, morpholino, pyrrolidinyl optionally substituted by C1-6acylamino,
    piperidino optionally substituted by hydroxy, C1-6 alkyl, carboxy, aminocarbonyl, N—(C1-6alkyl)aminocarbonyl, or N,N-di(C1-6alkyl)aminocarbonyl,
    or
    piperazinyl optionally substituted by C1-6 alkyl,
    C1-6 alkoxy optionally substituted by cyano, mono-, di- or tri-halogen, hydroxy, C1-6alkoxy, hydroxy C1-6 alkoxy, amino, N—(C1-6alkyl)amino, N,N-di(C1-6alkyl)amino, pyrrolyl, tetrazolyl, tetrahydropyranyl, morpholino, phthalimidyl, 2-oxo-1,3oxazolidinyl, phenyl, —C(O)—R201,
    pyrrolidinyl optionally substituted by C1-6acylamino,
    piperidino optionally substituted by hydroxy, C1-6 alkyl, carboxy, aminocarbonyl, N—(C1-6-alkyl)aminocarbonyl, or N,N-di(C1-6-alkyl)aminocarbonyl,
    or
    piperazinyl optionally substituted by C1-6 alkyl,
    wherein
    R201 represents hydroxy, amino, N—(C1-6alkyl)amino, N,N-di(C1-6alkyl)amino, N(halobenzyl)amino, C1-6 alkyl, C1-6 alkoxy, amino C2-6 alkylenyl, tetrazolyl, tetrahydropyranyl, morpholino,
    pyrrolidinyl optionally substituted by C1-6acylamino,
    piperidino optionally substituted by hydroxy, C1-6 alkyl, carboxy, aminocarbonyl, N—(C1-6alkyl)aminocarbonyl, or N,N-di(C1-6alkyl)aminocarbonyl,
    or
    piperazinyl optionally substituted by C1-6alkyl;
    R3 represents hydrogen, halogen, C1-6 alkyl optionally substituted by aminocarbonyl, arylC1-6 alkoxy, or mono-, di- or tri-halogen;
    R4 represents hydrogen or C1-6 alkyl;
    R5 represents hydrogen or C1-6 alkyl; and
    R6 represents hydrogen, halogen or C1-6 alkyl.


In another embodiment, the present invention provides the fused azolepyrimidine derivative of the formula (I), its tautomeric or stereoisomeric form, or a salt thereof: wherein

    X represents CR5R6 or NH;
    Y1 represents N;
    Y2 and Y3 represent CR3R4;
    Chemical bond between Y2═Y3 represents a single bond
    Z4 represents CH;
    Z1, Z2 and Z3 independently represent N, CH or CR2;
    R1 represents cyclopropyl, cyclopentyl, cyclohexyl, 2-furyl, 3-furyl, imidazolyl, pyrimidinyl, pyridazinyl, piperazinyl, 1,2,3-thiadiazolyl, 1,3-benzothiazolyl, quinolyl, 3H-imidazo[4,5-b]pyridinyl, 1H-pyrrol-2-yl optionally substituted by C1-6alkyl,
    
        1H-pyrrol-3-yl optionally substituted by C1-6alkyl, pyrazolyl optionally substituted by 1 or 2 C1-6alkyl, isoxazolyl optionally substituted by 1 or 2 C1-6alkyl,
        2-thienyl optionally substituted by chloro, nitro, cyano, or C1-6 alkyl, 3-thienyl optionally substituted by chloro, nitro, cyano, or C1-6 alkyl, piperidinyl optionally substituted by C1-6alkoxycarbonyl, or benzyloxy-carbonyl,
        phenyl optionally substituted by 1 to 3 substituents selected from the group consisting of fluoro, chloro, hydroxy, nitro, cyano, carboxy, C1-6 alkyl, C1-6alkoxy, C1-6alkoxycarbonyl, amino, N—(C1-6alkyl)amino, N—(C1-6acyl)amino, N—(C1-6alkoxycarbonyl)amino, N,N-di(C1-6alkyl)amino, N-(formyl)-N—C1-6alkyl amino, C1-6 alkylthio, C1-6alkanesulfonyl, sulfamoyl, pyrrolyl, imidazolyl, pyrazolyl, and piperazinyl optionally substituted by C1-6alkyl,
        pyridyl optionally substituted by 1 or 2 substituents selected from the group consisting of chloro, hydroxy, carboxy, C1-6alkoxy, C1-6alkylthio, amino, N—(C1-6alkyl)amino, N-(hydroxyC1-6alkyl)amino, N,N-di(C1-6alkyl)amino, N—(C1-6acyl)amino, N—(C1-6alkane)sulfonyl amino, N[N,N-di(C1-6alkyl)amino methylene]amino, and C1-6alkyl optionally substituted by tri halogen,
        pyrazinyl optionally substituted by C1-6alkyl,
        1,3-thiazolyl optionally substituted by 1 or 2 substituents selected from the group consisting of C1-6alkyl, pyridyl and N—(C1-6alkoxycarbonyl)amino, indolyl optionally substituted by C1-6alkyl,
        benzimidazolyl optionally substituted by C1-6alkyl or tri-halo C1-6alkyl, 1,2,3-benzotriazolyl optionally substituted by C1-6alkyl, 1,8-naphthyridinyl optionally substituted by
        C1-6alkyl optionally substituted by tri halogen,
        C1-6 alkyl optionally substituted by tri-halogen, phenyl, phenoxy, or thienyl,
        or
        C1-6alkoxy optionally substituted by phenyl, phenoxy, or thienyl;
        R2 represents fluoro, chloro, bromo, hydroxy, nitro, vinyl, cyano, amino, aminoacetoxy, N—(C1-6alkyl)amino, N,N-di(C1-6alkyl)amino, N-(hydroxyC1-6alkyl)-N—(C1-6alkyl)amino, 2-furyl, piperidino, morpholino, phenyl,
        
            pyrrolidinyl optionally substituted by acetamido,
            piperidino optionally substituted by hydroxy,
            piperazinyl optionally substituted by methyl, benzyl, C1-6alkoxycarbonyl, or aminocarbonyl,
            C1-6 alkyl optionally substituted by cyano, tri-fluoro, carboxy, methoxycarbonyl, aminocarbonyl, tert-butoxycarbonyl, tetrahydropyranyl, or morpholino,
            C1-6 alkoxy optionally substituted by hydroxy, cyano, methoxy, methoxycarbonyl, tert-butoxycarbonyl, carboxy, aminoacetyl, dimethylamino, aminocarbonyl, methylaminocarbonyl, dimethylaminocarbonyl, isopropylaminocarbonyl, fluorobenzylaminocarbonyl, cyclopropyl, pyrrolidinyl, piperidino, tetrahydropyranyl, morpholino, morpholinocarbonyl, 2-oxo-1,3-oxazolidin-4-yl, phthalimid-N-yl, or hydroxy C1-6 alkyleneoxy,
        
        
        R3 represents hydrogen;
        R4 represents hydrogen;
        R5 represents hydrogen; and
        R6 represents hydrogen.
    
    


In another embodiment, the present invention provides the fused azolepyrimidine derivative of the formula (I), its tautomeric or stereoisomeric form, or a salt thereof:

    X represents CR5R6 or NH;
    Y1 represents N;
    Y2 and Y3 represent CR3R4;
    Chemical bond between Y2═Y3 represents a single bond
    Z3 and Z4 represent CH;
    Z1 and Z2 independently represent CH or CR2;
    R1 represents 3H-imidazo[4,5-b]pyridinyl, benzimidazolyl pyridyl optionally substituted by hydroxy, amino, acetamido, methoxybenzyloxy or methylsulfonylamino,
    or
    
        1,3-thiazolyl optionally substituted by 1 or 2 methyl;
    
    
    R2 represents fluoro, chloro, bromo, morpholino, piperazinyl, methylpeperazinyl, methyl, tri-fluoro methyl, or
    
        C1-6 alkoxy optionally substituted by hydroxy, cyano, carboxy, dimethylaminocarbonyl, tetrahydropyranyl, morpholino, morpholinocarbonyl, tetrazolyl, or phthalimid-N-yl;
        R3 represents hydrogen;
        R4 represents hydrogen;
        R5 represents hydrogen; and
        R6 represents hydrogen.
    
    


In another embodiment, the present invention provides the fused azolepyrimidine derivative of the formula (I), its tautomeric or stereoisomeric form, or a salt thereof:

    wherein
    X represents CR5R6 or NH;
    Y1 represents N;
    Y2 and Y3 represent CR3R4;
    Chemical bond between Y2═Y3 represents a single bond.
    Z3 and Z4 represent CH;
    Z1 and Z2 independently represent CH or CR2;


In another embodiment, the present invention provides the fused azolepyrimidine derivative of the formula (I), its tautomeric or stereoisomeric form, or a salt thereof:

    X represents CR5R6 or NH;
    Y1 represents N;
    Y2 and Y3 represent CR3R4;
    Chemical bond between Y2═Y3 represents a single bond
    Z1 and Z4 represent CH;
    Z2 and Z3 independently represent CH or CR2;


In another embodiment, the present invention provides the fused azolepyrimidine derivative of the formula (I), its tautomeric or stereoisomeric form, or a salt thereof:

    X represents CR5R6or NH;
    Y1 represents N;
    Y2 and Y3 represent CR3R4;
    Chemical bond between Y2═Y3 represents a single bond;
    Z1, Z3 and Z4 represent CH;
    Z2 represents CR2;


The preferable compounds of the present invention are as follows:

    N-(7,8-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;
    2-(7,8-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1-pyridin-3-yl-ethylenol;
    N-(7,8-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1H-benzimidazole-5-carboxamide;
    6-(acetamido)-N-(7,8-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;
    N-{5-2-(7,8-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1-hydroxy-vinyl]pyridin-2-yl}acetamide;
    2-({5-[2-hydroxy-2-pyridin-3-ylvinyl]-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-8-yl}oxy)-N,N-dimethylacetamide;
    2-[7-methoxy-8-(tetrahydro-2H-pyran-2-ylmethoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-1-pyridin-3-ylethylenol;
    2-[8-(2-hydroxyethoxy)-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-1-pyridin-3-ylethylenol;
    ({5-[2-hydroxy-2-pyridin-3-ylvinyl]-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-8-yl}oxy)acetic acid;
    4-({5-[2-hydroxy-2-pyridin-3-ylvinyl]-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-8-yl}oxy)butanoic acid;
    ({5-[2-hydroxy-2-pyridin-3-ylvinyl]-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-8-yl}oxy)acetonitrile;
    2-[7-methoxy-8-(2H-tetrazol-5-ylmethoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-1-pyridin-3-ylethylenol;
    2-[7-methoxy-8-(4-morpholin-4-yl-4-oxobutoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-1-pyridin-3-ylethylenol;
    5-[1-hydroxy-2-(8-morpholin-4-yl-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)vinyl]pyridin-3-ol;
    N-(2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-5-hydroxynicotinamide;
    6-(acetamido)-N-(7,9-dimethoxy-8-methyl-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;
    N-(8,9-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-5-hydroxynicotinamide;
    5-hydroxy-N-(7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;
    N-(7,8-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-5-[(4-methoxybenzyl)oxy]nicotinamide;
    N-(7,8-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-5-hydroxynicotinamide;
    5-hydroxy-N-[8-(trifluoromethyl)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide;
    N-{8-[3-(1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)propoxy]-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl}nicotinamide;
    N-(7-bromo-8-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;
    6-amino-N-(8-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;
    1-(1H-benzimidazol-5-yl)-2-(8,9-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)ethylenol;
    2-(8,9-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1-(2,4-dimethyl-1,3-thiazol-5-yl)ethylenol;
    N-(9-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1H-benzimidazole-5-carboxamide;
    N-(8-bromo-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;
    N-(8-bromo-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1H-benzimidazole-5-carboxamide;
    N-(8-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1H-benzimidazole-5-carboxamide;
    N-(8-methyl-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1H-benzimidazole-5-carboxamide;
    N-[8-(trifluoromethyl)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-1H-benzimidazole-5-carboxamide;
    N-(7-fluoro-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1H-benzimidazole-5-carboxamide;
    N-(7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;
    N-(8-chloro-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1H-benzimidazole-5-carboxamide;
    6-(acetamido)-N-(8-morpholin-4-yl-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;
    1-(1H-benzimidazol-5-yl)-2-(8-morpholin-4-yl-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)ethylenol;
    N-{5-[1-hydroxy-2-(8-morpholin-4-yl-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)vinyl]pyridin-2-yl}acetamide;
    6-methyl-N-(8-morpholin-4-yl-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;
    1-(1H-benzimidazol-5-yl)-2-[8-(4-methylpiperazin-1-yl)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]ethylenol;
    N-(2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-3H-imidazo[4,5-b]pyridine-6-carboxamide;
    N-(7,8-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-3H-imidazo[4,5-b]pyridine-6-carboxamide;
    N-[7-(trifluoromethyl)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-1H-benzimidazole-5-carboxamide;
    N-(7,9-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1H-benzimidazole-5-carboxamide;
    N-{5-[2-(7,9dimethoxy-8-methyl-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1-hydroxyvinyl]pyridin-2-yl}acetamide;
    N-{5-[2-(7-bromo-9-methyl-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1-hydroxyvinyl]pyridin-2-yl}acetamide; and
    2-(8,9-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1-pyridin-3-ylethylenol;
    
        and its tautomeric or stereoisomeric form, pharmaceutically acceptable salts thereof.
    
    


Further, the present invention provides a medicament, which includes one of the compounds, described above and optionally pharmaceutically acceptable excipients.
Alkyl per se and “alk” and “alkyl” in alkane, alkoxy, alkanoyl, alkylamino, alkylaminocarbonyl, alkylaminosulphonyl, alkylsulphonylamino, alkoxycarbonyl, alkoxycarbonylamino and alkanoylamino represent a linear or branched alkyl radical having generally 1 to 6, preferably 1 to 4 and particularly preferably 1 to 3 carbon atoms, representing illustratively and preferably methyl, ethyl, propyl, isopropyl, isobutyl, tert-butyl, sec-butyl, pentyl, n-hexyl, and the like.
Alkylene represents the divalent linear or branched saturated hydrocarbon radical, consisting solely of carbon and hydrogen atoms, having generally 1 to 6 carbon preferably 1 to 4 and particularly preferably 1 to 3 carbon atoms, representing illustratively and preferably methylene, ethylene, 2-methyl-propylene, butylene, 2-ethylbutylene and the like.
Alkoxy illustratively and preferably represents methoxy, ethoxy, n-propoxy, iso-propoxy, tert-butoxy, n-pentoxy, n-hexoxy and the like.
Alkylamino represents an alkylamino radical having one or two (independently selected) alkyl substituents, illustratively and preferably representing methylamino, ethylamino, n-propylamino, isopropylamino, tert-butylamino, n-pentylamino, n-hexyl-amino, N,N-dimethylamino, N,N-diethylamino, N-ethyl-N-methylamino, N-methyl-N-n-propylamino, N-isopropyl-N-n-propylamino, N-t-butyl-N-methylamino, N-ethyl-N-n-pentylamino, N-n-hexyl-N-methylamino and the like.
Alkylamninocarbonyl represents an radical having one or two (independently selected) alkyl substituents, illustratively and preferably representing methylaminocarbonyl, ethylaminocarbonyl, n-propylaminocarbonyl, isopropylamino-carbonyl, tert-butyl-aminocarbonyl, n-pentylaminocarbonyl, n-hexylaminocarbonyl, N,N-dimethylaminocarbonyl, N,N-diethylaminocarbonyl, N-ethyl-N-methylaminocarbonyl, N-methyl-N-n-propylaminocarbonyl, N-isopropyl-N-n-propylaminocarbonyl, N-t-butyl-N-methylaminocarbonyl, N-ethyl-N-n-pentylamino-carbonyl, N-n-hexyl-N-methylaminocarbonyl and the like.
Alkylaminosulphonyl represents an alkylaminosulphonyl radical having one or two (independently selected) alkyl substituents, illustratively and preferably representing methylaminosulphonyl, ethylaminosulphonyl, n-propylaminosulphonyl, isopropylaminosulphonyl, tert-butylaminosulphonyl, n-pentylaminosulphonyl, n-hexyl-aminosulphonyl, N,N-diimethylaminosulphonyl, N,N-diethylaminosulphonyl, N-ethyl-N-methylamino-sulphonyl, N-methyl-N-n-propylaminosulphonyl, N-isopropyl-N-n-propylaminosulphonyl, N-t-butyl-N-methylaminosulphonyl, N-ethyl-N-n-pentylaminosulphonyl, N-n-hexyl-N-methylaminosulphonyl and the like.
Alkylsulphonyl illustratively and preferably represents methylsulphonyl, ethylsulphonyl, n-propylsulphonyl, isopropylsulphonyl, tert-butyl-sulphonyl, n-pentyl-sulphonyl, n-hexylsulphonyl and the like.
Alkoxycarbonyl illustratively and preferably represents methoxycarbonyl, ethoxycarbonyl, n-propoxycarbonyl, isopropoxycarbonyl, tert-butoxycarbonyl, n-pentoxycarbonyl, n-hexoxycarbonyl and the like.
Alkoxycarbonylamino illustratively and preferably represents methoxycarbonylamino, ethoxycarbonylamino, n-propoxycarbonylamino, isopropoxycarbonylamino, tert-butoxycarbonylamino, n-pentoxycarbonylamino, n-hexoxycarbonylamino and the like.
Alkanoylamino illustratively and preferably represents acetamido, ethylcarbonylamino and the like.
Cycloalkyl per se and in cycloalkylamino and in cycloalkylcarbonyl represents a cycloalkyl group having generally 3 to 8 and preferably 5 to 7 carbon atoms, illustratively and preferably representing cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl and the like.
Aryl per se and “aryl” in arylamino, arylcarbonyl, alkoxyaryl, represents a mono- to tricyclic aromatic carbocyclic radical having generally 6 to 14 carbon atoms, illustratively and preferably representing phenyl, naphthyl, phenanthrenyl and the like.
Arylamino represents an arylamino radical having one or two (independently selected) aryl substituents, illustratively and preferably representing phenylamino, diphenylamino naphthylamino and the like.
Heteroaryl per se and “heteroaryl” in heteroarylamino and heteroarylcarbonyl represents an aromatic mono- or bicyclic radical having generally 5 to 15 and preferably 5 or 6 ring atoms and up to 5 and preferably up to 4 hetero atoms selected from the group consisting of S, O and N, illustratively and preferably representing thienyl, furyl, pyrrolyl, thiazolyl, oxazolyl, imidazolyl, thiazolyl, pyrazinyl, pyridinyl, pyrimidinyl, pyridazinyl, thiophenyl, indolyl, isoindolyl, indazolyl, benzofuranyl, benzothiophenyl, quinolinyl, isoquinolinyl, 1,3 benzodioxole, benzofuranyl, benzofuran-2,5-diyl, benzofuran-3,5-diyl, and the like.
Heterocyclic per se and heterocyclic ring per se represent a mono- or polycyclic, preferably mono- or bicyclic, nonaromatic heterocyclic radical having generally 4 to 10 and preferably 5 to 8 ring atoms and up to 3 and preferably up to 2 hetero atoms and/or hetero groups selected from the group consisting of N, O, S, SO and SO2. The heterocyclyl radicals can be saturated or partially unsaturated. Preference is given to 5- to 8-membered monocyclic saturated heterocyclyl radicals having up to two hetero atoms selected from the group consisting of O, N and S, such as illustratively and preferably tetrahydrofuran-2-yl, pyrrolidin-2-yl, pyrrolidin-3-yl, pyrrolinyl, piperidinyl, morpholino, perhydroazepinyl.
Heterocyclylcarbonyl illustratively and preferably represents tetrahydrofuran-2-carbonyl, pyrrolidine-2-carbonyl, pyrrolidine-3-carbonyl, pyrrolinecarbonyl, piperidinecarbonyl, morpholinecarbonyl, perhydroazepinecarbonyl.
Halogen and Halo represents fluoro, chloro, bromo and/or iodo.
Further, the present invention provides a medicament which include one of the compounds described above and optionally pharmaceutically acceptable excipients.
EMBODIMENT OF INVENTION
The compound of the formula (I) of the present invention can be, but not limited to be, prepared by reactions described below. In some embodiments, one or more of the substituents, such as amino group, carboxyl group, and hydroxyl group of the compounds used as starting materials or intermediates are advantageously protected by a protecting group known to those skilled in the art. Examples of the protecting groups are described in “Protective Groups in Organic Synthesis (3nd Edition)” by Greene and Wuts.
The compound of the formula (I) of the present invention can be, but not limited to be, prepared by the Method [A], and [B] below.
The compound of the formula (I-a):




(wherein R1, R5, R6, Y1, Y2, Y3, Z1, Z2, Z3 and Z4 are the same as defined above) can be, but not limited to be, prepared by the following Method A.





The compound of formula (I-a) can be prepared, for example, by the reaction of the compound of formula (II) (wherein Y1, Y2, Y3, Z1, Z2, Z3 and Z4 are the same as defined above) with a compound of formula (III) (wherein R1, R5 and R6 are the same as defined above, and L represents C1-6 alkyl).
The reaction may be carried out without solvent, or in a solvent including, for instance, ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; amides such as N,N-dimethylformamide (DMF), N,N-dimethylacetamide and N-methylpyrrolidone; sulfoxides such as dimethylsulfoxide (DMSO); alcohols such as methanol, ethanol, 1-propanol, isopropanol and tert-butanol; water, and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction temperature can be optionally set depending on the compounds to be reacted. The reaction temperature is usually, but not limited to, about 10° C. to 200° C. and preferably about 50° C. to 160° C. The reaction may be conducted for, usually, 10 minutes to 48 hours and preferably 30 minutes to 24 hours.
Preparation of the Intermediates
The compound of formula (II′) (wherein Y1, Z1, Z2, Z3 and Z4 are the same as defined above, Y2 and Y3 independently represent CR3R4 or NR4 and are connected by single bond) and the compound of formula (II′′) (wherein Y1, Z1, Z2, Z3 and Z4 are the same as defined above, Y2 and Y3 independently represent CH or N and are connected by double bond) can be, but not limited to be, prepared by the following Method [A-i].




In the step 1, the compound of formula (II′) (wherein Y1, Z1, Z2, Z3 and Z4 are the same as defined above, Y2 and Y3 independently represent CR3R4 or NR4 and are connected by single bond) can be prepared, for example, by the reaction of the compound of formula (VI) (wherein Z1, Z2, Z3 and Z4 are the same as defined above) with an diaminoalkane derivatives such as ethylenediamine.
The reaction can be advantageously carried out using appropriate dehydrating agents such as SOCl2, POCl3, P2O5, P2S5, CS2 and others.
The reaction may be carried out without solvent, or in a solvent including for instance, ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction temperature is usually, but not limited to, about 10° C. to 200° C. and preferably about 50° C. to 200° C. The reaction may be conducted for, usually, 10 minutes to 48 hours and preferably 30 minutes to 24 hours.
In the step 2, the compound of formula (II′′) (wherein Y1, Z1, Z2, Z3 and Z4 are the same as defined above, Y2 and Y3 independently represent CH or N and are connected by double bond) can be prepared, for example, from the compound of formula (II′) (wherein Y1, Z1, Z2, Z3 and Z4 are the same as defined above, Y2 and Y3 independently represent CR3R4 or NR4 and are connected by single bond) by the oxidation reaction using an agent such as MnO2, KMnO4 and others, or by the dehydrogenation reaction using palladium on carbon.
The reaction can be carried out in a solvent including, for instance, ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; dimethylformamide (DMF), dimethylacetamide(DMAC), 1,3-dimethyl-3,4,5,6-tetrahydro-2(1H)-pyrimidinone (DMPU), 1,3-dimethyl-2-imidazolidinone (DMI), N-methylpyrrolidinone (NMP), and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction temperature is usually, but not limited to, about 0° C. to 200° C. and preferably about 50° C. to 200° C. The reaction may be conducted for, usually, 30 minutes to 48 hours and preferably 2 hours to 24 hours.
The compound of formula (VI) is commercially available or can be synthesized by conventional method.
The compound of formula (III) can be prepared, for example, by the following Method [A-ii].




The compound of formula (III) (wherein L, R1, R5 and R6 are the same as defined above) can be prepared by the reaction of the compound of formula (VII) (wherein R1, R5 and R6 are the same as defined above) with the compound of formula (VIII) (wherein L is the same as defined above) in the presence of a base such as potassium hydride, potassium hexamethyldisilazide, and others.
The reaction can be carried out in a solvent including, for instance, ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene, dimethylformamide (DMF), dimethylacetamide(DMAC), 1,3-dimethyl-3,4,5,6-tetrahydro-2(1H)-pyrimidinone (DMPU), 1,3-dimethyl-2-imidazolidinone (DMI), N-methylpyrrolidinone (NMP), and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction temperature is usually, but not limited to, about −100° C. to 100° C. The reaction may be conducted for, usually, 30 minutes to 48 hours and preferably 2 hours to 12 hours.
Alternatively, the compound of formula (III) can be prepared, for example, by the following Method [A-iii].




The compound of formula (II) (wherein L, R1, R5 and R6 are the same as defined above) can be prepared by the reaction of the compound of formula (IX) (wherein R1 is the same as defined above and L′ is a leaving group such as halogen atom e.g., chlorine or bromine atom, or imidazole) with the compound of formula (X) (wherein wherein L, R5 and R6 are the same as defined above) or its salts, for example, potassium salt.
The reaction can be carried out in the presence of Lewis acid including magnesium salts, such as magnesium bromide, magnesium chloride, magnesium iodide, magnesium acetate, and others or a base such as n-butyl lithium, sec-butyl lithium, and others. The reaction can be carried out in a solvent including, for instance, ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene, and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The preparation of the compound formula (I-b):




(wherein R1, Y1, Y2, Y3, Z1, Z2, Z3 and Z4 are the same as defined above) can be, but not limited to be, prepared by the following Method B.





The compound of formula (I-b) can be prepared, for example, by the reaction of the compound of formula (IV) (wherein Y1, Y2, Y3, Z1, Z2, Z3 and Z4 are the same as defined above) with a compound of formula (V) (wherein R1 is the same as defined above and L′′ is a leaving group, such as hydroxy; halogen atom e.g., chlorine, bromine, or iodine atom; imidazole or,




wherein R1 is the same as defined above). In the case L′′ is hydroxy, the reaction can be advantageously carried out by using a coupling agent such as benzotriazole-1-yl-oxy-tris-pyrrolidino-phosphonium hexafluorophosphate (PyBOP), 1,1′-carbonyldi(1,3-imiazole)(CDI), 1,1′-carbonyldi(1,2,4-triazole)(CDT) and others.

In the case L′′ is halogen atom, imidazole, or




the reaction can be advantageously conducted in the presence of a base, including, for instance, such as pyridine, triethylamine and N,N-diisopropylethylamine, dimethylaniline, diethylaniline, and others.

The reaction may be carried out without solovent, or in a solvent including, for instance, ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitrites such as acetonitrile; amides such as N,N-dimethylformamide (DMF), N,N-dimethylacetamide (DMAC) and N-methylpyrrolidone (NMP); urea such as 1,3-dimethyl-2-imidazolidinone (DMI); sulfoxides such as dimethylsulfoxide (DMSO); and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction temperature is usually, but not limited to, about 40° C. to 200° C. and preferably about 20° C. to 180° C. The reaction may be conducted for, usually, 30 minutes to 48 hours and preferably 2 hours to 12 hours.
Preparation of Intermediates
The compound of formula (IV) can be, but not limited to be, prepared by the following Method [B-i]:




The compound of formula (IV) (wherein Y1, Y2, Y3, Z1, Z2, Z3 and Z4 are the same as defined above) can be prepared by the reaction of compound of formula (II) (wherein Y1, Y2, Y3, Z1, Z2, Z3 and Z4 are the same as defined above) with cyanogen halides such as cyanogen bromide.
The reaction may be carried out in a solvent including, for instance, ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; amides such as N,N-dimethylformamide (DMF), N,N-dimethylacetamide and N-methylpyrrolidone; alcohols such as methanol, ethanol, 1-propanol, isopropanol and tert-butanol; and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction temperature is usually, but not limited to, about −10+ C. to 200° C. The reaction may be conducted for, usually, 30 minutes to 48 hours and preferably 1 hour to 24 hours.
The compound of formula (II) (wherein Y1, Y2, Y3, Z1, Z2, Z3 and Z4 are the same as defined above) can be obtained in the same manner described in Method [A-i].
The compound of formula (VII), (VIII), (IX) and (X) are commercially available or can be synthesized by conventional method.
When the compound shown by the formula (I) or a salt thereof has an asymmetric carbon(s) in the structure, their optically active compounds and racemic mixtures are also included in the scope of the present invention.
Typical salts of the compound shown by the formula (I) include salts prepared by the reaction of the compound of the present invention with a mineral or organic acid, or an organic or inorganic base. Such salts are known as acid addition and base addition salts, respectively.
Acids to form acid addition salts include inorganic acids such as, without limitation, sulfuric acid, phosphoric acid, hydrochloric acid, hydrobromic acid, hydroiodic acid and the like, and organic acids, such as, without limitation, p-toluenesulfonic acid, methanesulfonic acid, oxalic acid, p-bromophenylsulfonic acid, carbonic acid, succinic acid, citric acid, benzoic acid, acetic acid, and the like.
Base addition salts include those derived from inorganic bases, such as, without limitation, ammonium hydroxide, alkaline metal hydroxide, alkaline earth metal hydroxides, carbonates, bicarbonates, and the like, and organic bases, such as, without limitation, ethanolamine, triethylamine, tri(hydroxymethyl)aminomethane, and the like. Examples of inorganic bases include, sodium hydroxide, potassium hydroxide, potassium carbonate, sodium carbonate, sodium bicarbonate, potassium bicarbonate, calcium hydroxide, calcium carbonate, and the like.
The compound of the present invention or a salts thereof, depending on its substituents, may be modified to form lower alkylesters or known other esters; and/or hydrates or other solvates. Those esters, hydrates, and solvates are included in the scope of the present invention.
The compound of the present invention may be administered in oral forms, such as, without limitation normal and enteric coated tablets, capsules, pills, powders, granules, elixirs, tinctures, solution, suspensions, syrups, solid and liquid aerosols and emulsions. They may also be administered in parenteral forms, such as, without limitation, intravenous, intraperitoneal, subcutaneous, intramuscular, and the like forms, well-known to those of ordinary skill in the pharmaceutical arts. The compounds of the present invention can be administered in intranasal form via topical use of suitable intranasal vehicles, or via transdermal routes, using transdermal delivery systems well-known to those of ordinary skilled in the art.
The dosage regimen with the use of the compounds of the present invention is selected by one of ordinary skill in the arts, in view of a variety of factors, including, without limitation, age, weight, sex, and medical condition of the recipient, the severity of the condition to be treated, the route of administration, the level of metabolic and excretory function of the recipient, the dosage form employed, the particular compound and salt thereof employed.
The compounds of the present invention are preferably formulated prior to administration together with one or more pharmaceutically-acceptable excipients. Excipients are inert substances such as, without limitation carriers, diluents, flavoring agents, sweeteners, lubricants, solubilizers, suspending agents, binders, tablet disintegrating agents and encapsulating material.
Yet another embodiment of the present invention is pharmaceutical formulation comprising a compound of the invention and one or more pharmaceutically-acceptable excipients that are compatible with the other ingredients of the formulation and not deleterious to the recipient thereof. Pharmaceutical formulations of the invention are prepared by combining a therapeutically effective amount of the compounds of the invention together with one or more pharmaceutically-acceptable excipients. In making the compositions of the present invention, the active ingredient may be mixed with a diluent, or enclosed within a carrier, which may be in the form of a capsule, sachet, paper, or other container. The carrier may serve as a diluent, which may be solid, semi-solid, or liquid material which acts as a vehicle, or can be in the form of tablets, pills, powders, lozenges, elixirs, suspensions, emulsions, solutions, syrups, aerosols, ointments, containing, for example, up to 10% by weight of the active compound, soft and hard gelatin capsules, suppositories, sterile injectable solutions and sterile packaged powders.
For oral administration, the active ingredient may be combined with an oral, and non-toxic, pharmaceutically-acceptable carrier, such as, without limitation, lactose, starch, sucrose, glucose, sodium carbonate, mannitol, sorbitol, calcium carbonate, calcium phosphate, calcium sulfate, methyl cellulose, and the like; together with, optionally, disintegrating agents, such as, without limitation, maize, starch, methyl cellulose, agar bentonite, xanthan gum, alginic acid, and the like; and optionally, binding agents, for example, without limitation, gelatin, natural sugars, beta-lactose, corn sweeteners, natural and synthetic guns, acacia, tragacanth, sodium alginate, carboxymethylcellulose, polyethylene glycol, waxes, and the like; and, optionally, lubricating agents, for example, without limitation, magnesium stearate, sodium stearate, stearic acid, sodium oleate, sodium benzoate, sodium acetate, sodium chloride, talc, and the like.
In powder forms, the carrier may be a finely divided solid which is in admixture with the finely divided active ingredient. The active ingredient may be mixed with a carrier having binding properties in suitable proportions and compacted in the shape and size desired to produce tablets. The powders and tablets preferably contain from about 1 to about 99 weight percent of the active ingredient which is the novel composition of the present invention. Suitable solid carriers are magnesium carboxymethyl cellulose, low melting waxes, and cocoa butter.
Sterile liquid formulations include suspensions, emulsions, syrups and elixirs. The active ingredient can be dissolved or suspended in a pharmaceutically acceptable carrier, such as sterile water, sterile organic solvent, or a mixture of both sterile water and a sterile organic solvent.
The active ingredient can also be dissolved in a suitable organic solvent, for example, aqueous propylene glycol. Other compositions can be made by dispersing the finely divided active ingredient in aqueous starch or sodium carboxymethyl cellulose solution or in a suitable oil.
The formulation may be in unit dosage form, which is a physically discrete unit containing a unit dose, suitable for administration in human or other mammals. A unit dosage form can be a capsule or tablets, or a number of capsules or tablets. A “unit dose” is a predetermined quantity of the active compound of the present invention, calculated to produce the desired therapeutic effect, in association with one or more excipients. The quantity of active ingredient in a unit dose may be varied or adjusted from about 0.1 to about 1000 milligrams or more according to the particular treatment involved.
Typical oral dosages of the present invention, when used for the indicated effects, will range from about 0.01 mg/kg/day to about 100 mg/kg/day, preferably from 0.1 mg/kg/day to 30 mg/kg/day, and most preferably from about 0.5 mg/kg/day to about 10 mg/kg/day. In case of parenteral administration, it has generally proven advantageous to administer quantities of about 0.001 to 100 mg/kg/day, preferably from 0.01 mg/kg/day to 1 mg/kg/day. The compounds of the present invention may be administered in a single daily dose, or the total daily dose may be administered in divided doses, two, three, or more times per day. Where delivery is via transdermal forms, of course, administration is continuous.
EXAMPLES
The present invention will be described in detail below in the form of examples, but they should by no means be construed as defining the metes and bounds of the present invention.
In the examples below, all quantitative data, if not stated otherwise, relate to percentages by weight.
1H NMR spectra were recorded using either Bruker DRX-300 (300 MHz for 1H) spectrometer or Brucker 500 UltraShieledTM (500 MHz for 1 H). Chemical shifts are reported in parts per million (ppm) with tetramethylsilane (TMS) as an internal standard at zero ppm. Coupling constant (J) are given in hertz and the abbreviations s, d, t, q, m, and br refer to singlet, doublet, triplet, quartet, multiplet, and broad, respectively. The mass determinations were carried out by MAT95 (Finnigan MAT).
Liquid Chromatography—Mass spectroscopy (LC-MS) data were recorded on a Micromass Platform LC with Shimadzu Phenomenex ODS column(4.6 mm φ×30 mm) flushing a mixture of acetonitrile-water (9:1 to 1:9) at 1 ml/min of the flow rate. Mass spectra were obtained using electrospray (ES) ionization techniques (Micromass Platform LC). TLC was performed on a precoated silica gel plate (Merck silica gel 60 F-254). Silica gel (WAKO-gel C-200 (75-150 μm)) was used for all column chromatography separations. All chemicals were reagent grade and were purchased from Sigma-Aldrich, Wako pure chemical industries, Ltd., Tokyo kasei kogyo Co., Ltd., Nacalai tesque, Inc., Watanabe Chemical Ind. Ltd., Maybridge plc, Lancaster Synthesis Ltd., Merck KgaA, Kanto Chemical Co., Ltd.
The effects of the compounds of the present invention were examined by the following assays.
[Determination of IC50 Values of Compounds in Kinase Assay of PI3Kγ]
Chemicals and Assay Materials
Phosphatidylinositol (PtdIns) and phosphatidylserine (PtdSer) were purchased from DOOSAN SERDARY RESEARCH LABORATORIES (Toronto, Canada). Recombinant human PI3Kγ (full length human PI3K p110γ fused with a His6-tag at the C-terminus expressed in S. frugiperda 9 insect cells) was obtained from ALEXIS BIOCHEMICALS (#201-055-C010; San Diego, Calif.). [γ33P]ATP and unlabeled ATP were purchased from AMBRSHAM PHARMACIA BIOTECH (Buckinghamshire, UK) and ROCHE DIAGNOSTICS (Mannheim, Germany), respectively. Scintillation cocktails and MicroScint PSTM were purchased from PACKARD (Meriden, Conn.). MaxisorpTM plates were purchased from NALGE NUNC INTERNATIONAL K.K. (Tokyo, Japan). All other chemicals not further specified were from WAKO PURE CHEMICALS (Osaka, Japan).
Solid-Phase Lipid Kinase Assay
To assess inhibition of PI3Kγ by compounds, the MaxisorpTM plates were coated with 50 μl/well of a solution containing 50 μg/ml PtdIns and 50 μg/ml PtdSer dissolved in chloroform:ethanol (3:7). The plates were subsequently air-dried by incubation for at least 2 hours in a fume hood. The reaction was set up by mixing 25 μl/well of assay buffer 2× (100 mM MOPSO/NaOH, 0.2 M NaCl, pH 7.0, 8 mM MgCl2, 2 mg/ml BSA (fatty acid-free)) and 50 ng/well PI3Kγ in the lipid pre-coated plate and 10× test compounds were added in 2% DMSO. The reaction was started by adding 20 μl/well of ATP mix (final 10 μM ATP; 0.05 μCi/well [γ33P]ATP). After incubation at RT for 2 hours, the reaction was terminated by adding 50 μl/well stop solution (50 mM EDTA, pH 8.0). The plate was then washed twice with Tris-buffered saline (TBS, pH 7.4). MicroScint PSTM (PACKARD) scintillation mix was added at 100 μl/well, and radioactivity was counted by using a TopCountTM (PACKARD) scintillation counter.
The inhibition percent at each concentration of compound was calculated, and IC50 values were determined from the inhibition of curve.
[Isozyme Selectivity Test in PI3K]
{Determination of IC50 Values of Compounds in Kinase Assay of PI3Kβ}
Recombinant baculovirus of PI3Kβ p110β and GST-p85α were obtained from Dr. Katada (University of Tokyo). Recombinant PI3K heterocomplex of p110β and GST-p85α were co-expressed in insect cells according to manufacture's instruction (Pharmingen, San Diego, Calif.), and purified with glutathione affinity column. Kinase assay of PI3Kβ was prepared in a similar manner as described in the part of [Determination of IC50 values of compounds in kinase assay of PI3Kγ].
[Selectivity Test with Other Kinases]
Kinase selectivity of the compounds was assessed by using a few kinase assaies such as kinase assay of Syk.
{Syk Tyrosine Kinase Inhibitory Assay for Selectivity}
(1) Preparation of Syk Protein
A cDNA fragment encoding human Syk openreading frame was cloned from total RNA of human Burkitt's lymphoma B cell lines, Raji (American Type Culture Collection), with the use of RT-PCR method. The cDNA fragment was inserted into pAcG2T (Pharmingen, San Diego, Calif.) to construct a baculovirus transfer vector. Then the vector, together with the linearized baculovirus (BaculoGoldTM, Pharmingen), was used to transfect Sf21 cells (Invitrogen, San Diego, Calif.).
Generated recombinant baculovirus was cloned and amplified in Sf21 cells. Sf21 cells were infected with this amplified high titer virus to produce a chimeric protein of Syk kinase fused by glutathione-S-transferase (GST).
The resulting GST-Syk was purified with the use of glutathione column (Amersham Pharmacia Biotech AB, Uppsala, Sweden) according to the manufacturer's instruction. The purity of the protein was confirmed to be more than 90% by SDS-PAGE.
(2) Synthesize of a Peptide
Next, a peptide fragment of 30 residues including two tyrosine residues, KISDFGLSKALRADENYYKAQTHGKWPVKW, was synthesized by a peptide synthesizer. The N-terminal of the fragment was then biotinylated to obtain biotinylated activation loop peptide (AL).
(3) The Measurement of Syk Tyrosine Kinase Activity
All reagents were diluted with the Syk kinase assay buffer (50 mM Tris-HCl (pH 8.0), 10 mM MgCl2, 0.1 mM Na3VO4, 0.1% BSA, 1 mM DTT). First, a mixture (35 μl) including 3.2 μg of GST-Syk and 0.5 μg of AL was put in each well in 96-well plates. Then 5 μl of a test compound in the presence of 2.5% dimethyl sulfoxide (DMSO) was added to each well. To this mixture was added 300 μM ATP (10 μl) to initiate the kinase reaction. The final reaction mixture (50 μl) consists of 0.65 nM GST-Syk, 3 μM AL, 30 μM ATP, a test compound, 0.25% DMSO, and a Syk kinase assay buffer.
The mixture was incubated for 1 hour at room temperature (RT), and the reaction was terminated by the addition of 120 μl of termination buffer (50 mM Tris-HCl (pH 8.0), 10 mM EDTA, 500 mM NaCl, 0.1% BSA). The mixture was transferred to streptavidin-coated plates and incubated for 30 minutes. at room temperature to combine biotin-AL to the plates. After washing the plates with Tris-buffered saline (TBS) (50 mM Tris-HCl (pH 8.0), 138 mM NaCl, 2.7 mM KCl) containing 0.05% Tween-20 for 3 times, 100 μl of antibody solution consisting of 50 mM Tris-HCl (pH 8.0), 138 mM NaCl, 2.7 mM KCl, 1% BSA, 60 ng/ml anti-phosphotyrosine mono-clonal antibody, 4G10 (Upstate Biotechnology), which was labeled with europium by Amersham Pharmacia's kit in advance, was added and incubated at room temperature for 60 minutes. After washing, 100 μl of enhancement solution (Amersham Pharmacia Biotech) was added and then time-resolved fluorescence was measured by multi-label counter ARVO (Wallac Oy, Finland) at 340 nm for excitation and 615 nm for emission with 400 msec of delay and 400 msec of window.
[Determination of IC50 Values of Compounds in Superoxide Generation from Human Peripheral Mononuclear Cells]
Blood (100 ml/donor) was taken from healthy human volunteers by venepuncture with 50 ml syringes containing 50 units heparin. Red blood cells were removed by incubation with 1% (w/v) dextran and 0.45% (w/v) glucose for 30 minutes at room temperature. After centrifugation at 350× g for 10 minutes, the cell pellet was resuspended in 10 ml PBS. The cell suspension was gently layered on 20 ml of 60% and 20 ml of 80% Percoll (Amersham Pharmacia Biotech, Sweden) gradient in PBS in 50 ml tube (#2335-050, Iwaki, Japan). After centrifugation at 400× g for 30 minutes at room temperature, peripheral polymorphonuclear leukocytes (PMNs) were obtained from the interference between 60% and 80% Percoll phases. After twice washing in PBS, PMNs were suspended at a density of 107 cells/ml in Hank's Balanced Salt Solution (HBSS: Nissui, Japan) supplemented by 10 mM Na-Hepes (pH 7.6), 0.1% BSA and kept on ice until furter use.
To test inhibition of formyl-methionyl-leucyl-phenylalanine (fMLP)-induced superoxide generation by compounds, PMNs (2×105 cells/well) were seeded in HBSS, 10 mM Na-Hepes (pH 7.6), 0.1% BSA in 96-well clear bottom black plate (Cat.#3904, Costar) and pretreated with luminol (1 μg/well; Sigma) and test compounds for 10 minutes at 37° C. fMLP peptide (Cat.#4066; Peptide Institute Inc, Japan) was prepared in 10 μM in the same buffer and prepared in a polypropylene plate (Cat.#3365, Coster). Chemiluminescence (CL) was measured by FDSS-6000 (Hamamatsu Photonics) over 15 minutes after stimulation with 1 μM fMLP. The percentage of inhibition at each concentration of compound was calculated based on the first peak of CL at approximately 1 minute after addition of stimulus and IC50 values were determined from the inhibition curve.
For opsonized zymosan (OZ) and phorbol 12-myristate 13-acetate (PMA) stimulation, Zymosan A (Sigma) was suspended in HBSS at a concentration of 1 mg/ml and incubated with human pooled serum at a final concentration range of 9 to 80% at 37° C. for 30 minutes to opsonize the zymosan, followed by centrifugation at 500× g for 10 minutes at 4° C. Then the sediments were washed twice in HBSS and finally resuspended in HBSS to a concentration between 1 and 10 mg/ml. Opsonized zymosan (OZ) was used at 5 mg/ml for stimulation. Phorboll2-myristate 13-acetate (PMA) was initially dissolved at a concentration of 0.1 mg/ml in DMSO as a stock solution and stored frozen at −20° C. PMA solution was prepared from the stock solution by further dilution in HBSS to the concentration of 100 ng/ml. PMNs (2×105 cells/well) were seeded in HBSS, 10 mM Na-Hepes (pH 7.6), 0.1% BSA in 96-well white plate (Packard) and pretreated with luminol (1 μg/well; Sigma) and test compounds for 10 minutes at 37° C. CL was measured by Arvo counter (Wallac)) at 30 minutes after the stimulation with OZ or PMA. The percentage of inhibition at each concentration of compound was calculated and IC50 values were determined from the inhibition curve.
[Determination of IC50 Values of Compounds in Elastase Release from Human Peripheral Mononuclear Cells]
To test inhibition of elastase release by compounds, PMNs (5×105 cells/well) were seeded in HBSS supplemented with 10 mM Na-Hepes (pH 7.6), 0.1% BSA in 96-well plate. Cells were pretreated with cytochalasine B (0.1 μg/well; Nakarai, Japan) and test compounds in 90 μl/well for 10 minutes at 37° C. Cells were stimulated with 1 μM fMLP for 15 minutes at 37° C. Supernatants (40 μl/well) were collected into 384 well black plate (Packard) to measure elastase activity. Fluorescent-based elastase reaction was started by the addition of 10 μl of 0.5 mM Suc-Ala-Ala-Ala-MCA (Cat. #3133v; Peptide Institute Inc, Japan) into the 384 well plate at room temperature. The fluorescence emission was measured at 460 nm (γex, 360 nm) by using a Wallac-Arvo counter (PerkinElmer, Boston, Mass.) fluorescence plate leader for 120 minutes. IC50 values of compounds were determined at the initial velocity of the reaction.
[Determination of IC50 Values of Compounds in Chemotaxis Assay with the Use of Human PMNs]
Freshly prepared PMNs (1.1×107 cells/ml) were incubated with compounds in a polypropylene 96 well plate (Cat.#3365, Coster) for 10 minutes in HBSS supplemented with 10 mM Na-Hepes (pH 7.6), 0.1% BSA. Cells (100 μl) were incubated with test compounds or vehicle for 30 minutes and were transferred into an Multiwell insert (Cat.# 351183; Falcon) 24w plate. FMLP (10 nM, 0.5 ml) was added into the lower chamber of the plate, and chemotaxis was measured in CO2 incubator at 37° C. for 1 hour. Migrated cells were counted using FACScan (Becton Dickinson, Franklin Lakes, N.J.). The percentage of inhibition at the each concentration of compound was calculated, and the IC50 values were determined from the inhibition curve.
[Determination of IC50 Values of Compounds in Chemotaxis Assay with the Use of Transfectants]
(1) Cell
Human CCR3-transformed L1.2 cells were used. Human CCR3-expressing L1.2 stable transformant was established by electroporation, referring to the methods described in J. Exp. Med. 183:2437-2448, 1996. The human CCR3-transformed L1.2 cells were maintained in RPMI-1640 supplemented with 10% FCS, 100 units/ml of penicillin G and 100 μg/ml of streptomycin, and 0.4 mg/ml of Geneticin. One day before the chemotaxis assay, cells were pretreated with 5 mM sodium butyrate—containing culture medium (5×105 cells/ml) for 20-24 hours to increase the expression of CCR3.
(2) Chemotaxis Assay
Butyrate-pretreated cells were suspended in chemotaxis buffer (Hanks' solution Cat.#05906 Nissui, 20 mM HEPES pH 7.6, 0.1% human serum albumin Cat.#A-1887 Sigma) at a cell density of 1.1×107 cells/ml. A mixture of 90 μl of cell suspension and 10 μl of compound solution diluted with chemotaxis buffer (10-times concentration of the final concentration) were preincubated for 10 minutes at 37° C. The mixture of cells and compounds was added into the upper chamber of the 24-well chemotaxis chamber (TranswellTM, Cat.#3421, Costar, pore size;5 μm). 0.5 ml of 10 nM of human recombinant eotaxin (Cat.#23209, Genzyme Techne) solution, diluted with chemotaxis buffer, was added into the lower chamber of the chemotaxis plate. Then, chemotaxis was performed in CO2 incubator at 37° C. for 4 hours. After 4 hours incubation, migrated cells were counted using FACScan (Becton Dickinson). The percentage of inhibition at the each concentration of compound was calculated, and IC50 values were determined from the inhibition curve.
[Mouse fMLP-Induced Pleurisy Model]
Seven weeks old BALB/c female mice were divided into 3 groups, a nontreatment group, a vehicle group and a treatment group. Mice in the treated group were first injected intravenously with compounds of the present invention at varied doses. Mice in the vehicle group were injected with vehicle containing 10% Cremophor EL (Nacalai Tesque) in saline. Three minutes after the treatment, a solution containing 1 mg/mouse of fMLP in 3.3% DMSO in PBS was administrated intrapleuraly into a vehicle group and a treated group mice. Four hours after fMLP-injection, mice were sacrificed and pleural fluid was collected by washing the pleural cavity twice with 2 ml PBS. Total cells per milliliter of pleural fluid were counted using a hema-cytometer. Cell differentiation of pleural fluid was determined by counting a minimum of 200 cells from a Giemsa's-stained cytospin slide preparation. Statistical analysis was performed by means of Student's t-test for paired data or analysis of variance with Dunnett's Post test , using GraphPadPRISM for Windows, version 2.01.
For practical reasons, the compounds are grouped in some classes of activity as follows:
In vitro IC50=A(= or <)0.1 μM<B(= or <)0.5 μM<C(= or <)2 μM<D
The compounds of the present invention also show strong activity in vivo assays.
(dec.) in the following tables represents decomposition.
Example 1-1
Z)-2-(8,9-Dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1-(3-pyridinyl)ethenol
(1) Methyl 3-oxo-3-(3-pyridinyl)propanoate




A 0.5 M solution of pottasium hexamethyldisilazide in toluene (22 ml, 11 mmol) was mixed with tetrahydrofuran (5 ml), and the mixture was cooled at −78° C. To the cold (−78° C.) mixture was added dropwise a solution of 3-acethylpyridine (1.0 g, 8.26 mmol) in tetrahydrofuran (5 ml). The mixture was warmed to room temperature and stirred for 3 hours. The mixture was cold at −78° C., and then dimethyl carbonate (1.2 ml, 14.3 mmol) was added dropwise. The resulting solution was allowed to warm to room temperature and stirred overnight. The reaction solution was quenched by adding aqueous 1N HCl solution, and extracted three times with ethyl acetate. The combined organic layers were washed with water and brine, dried over magnesium sulfate, filtrated, and concentrated under reduced pressure. The residue was purified by column chromatography on silica-gel hexane/ethyl acetate, 1/1) to give methyl 3-oxo-3-(3-pyridinyl)propanoate (1.0 g, 68% yield) as an oil.
(2) 2-(4,5-Dihydro-1H-imidazol-2-yl)-4,5-dimethoxyaniline




2-Amino-4,5-dimethoxybenzonitrile (5.0 g, 28 mmol) was added to ethylenediamine (7.9 g, 131 mmol) at room temperature. The resulting solution was warmed to 40, and a catalitic amount of diphosphorus pentasulfide (50 mg) was added. The mixture was heated to 80-90, and the stirring was continued overnight. The reaction mixture was diluted with water, and the resulting precipitate was collected by filtration to give 2-(4,5-dihydro-1H-imidazol-2-yl)-4,5-dimethoxyaniline (5.1 g, 82%) as a solid.

(3) (Z)-2-(8,9-Dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1-(3-pyridinyl)ethenol




A mixture of 2-(4,5-dihydro-1H-imidazol-2-yl)-4,5-dimethoxyaniline (0.15 g, 0.68 mmol) and methyl-3-oxo-3(3-pyridinyl)propanoate (0.20 g, 1.12 mmol) was stirred at 155. for 1 hour. The reaction mixture was purified by column chromatography on silica-gel (dichloromethane/methanol, 25/1) to give (Z)-2-(8,9-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1-(3-pyridinyl)ethenol (66.9 mg, 28%) as a yellow solid.
Melting point: 275° C. Mass spectrometry: 351 In vitro PI3K-β inhibitory activity: C In vitro PI3K-γ inhibitory activity: A 1H-NMR (500. MHz, DMSO-d6): d 3.79 (3H, s), 3.88 (3H, s), 3.98-4.08 (4H, m), 5.63 (1H, s), 7.13 (1H, s), 7.24 (1H, s), 7.50 (1H, dd, J=4.7, 7.8 Hz), 8.27 (1H, dt, J=1.6, 7.8 Hz), 8.67 (1H, dd, J=1.6, 4.7 Hz), 9.13 (1H, d, J=1.6 Hz), 13.9 (1H, bs).
Example 1-2
(Z)-2-(8,9-Dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1-(3-pyridinyl)-ethenol hydrochloride




To a solution of (Z-2-(8,9-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1-(3-pyridinyl)ethenol (16.8 mg, 0.05 mmol)) in dioxane (15 ml) at room temperature was added aqueous 6N HCl solution (0.05 ml). After being stirred for 30 minutes, the mixture was dried under reduced pressure to give (Z)-2-(8,9-dimethoxy-2,3-di-hydroimidazo[1,2-c]quinazolin-5-yl)-1-(3-pyridinyl)ethenol hydrochloride (18.5 mg, quantitative) as a yellow solid.
Melting point: >300° C. Mass spectrometry: 351 In vitro PI3K-β inhibitory activity: C In vitro PI3K-γ inhibitory activity: A 1H-NMR (500 MHz, DMSO-d6): δ 3.88 (3H, s), 4.00 (3H, s), 4.22 (2H, t, J =9.1 Hz), 4.55 (2H, t, J=9.1 Hz), 6.21 (1H, s), 7.60 (1H, s), 7.66 (1H, dd, J=4.7, 8.2 Hz), 7.90 (1H, s), 8.47 (1H, d, J=8.2 Hz), 8.79 (1H, d, J=4.7 Hz), 9.28 (1H, s), 14.9 (1H, bs).
Example 1-3
2-[7-Methoxy-8-(methoxymethoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-1-pyridin-3-ylethylenol
(1) 4-Formyl-2-methoxy-3-nitrophenyl acetate




By the procedure described in U.S. Pat. No. 4,287,341 or J. Chem. Soc. 376 (1948), vanillin acetate 5.00 g afforded the title compound 4.54 g as yellow solid. Yield 73.6%.
H-NMR (500 MHz, DMSO-d6) δ: 2.40 (s 3H), 3.87 (s 3H), 7.75 (d 1H J=8.4 Hz), 7.94 (d 1H J=8.4 Hz), 9.90 (s 1H)
(2) 4-Hydroxy-3-methoxy-2-nitrobenzaldehyde




A mixture of 4-formyl-2-methoxy-3-nitrophenyl acetate 4.54 g (19.0 mmol) and potassium carbonate 5.24 g (37.9 mmol) in methanol 40 mL was stirred at room temperature for 2 hours. The reaction mixture was poured into water, acidified by 1N HCl solution and extracted into AcOEt. The organic layer was washed with brine, dried over MgSO4, filtrated and the solvent was evaporated. The residue was washed with n-hexane to give the title compound 3.60 g as white solid. Yield 96.3%.
(3) 4-Hydroxy-3-methoxy-2-nitrobenzonitrile




To a mixture of 4-hydroxy-3-methoxy-2-nitrobenzaldehyde 14.5 g (73.5 mmol) in 28% ammonia solution 150 mL and tetrahydrofuran 15 mL was added iodine 22.4 g (88.2 mmol) and stirred at room temperature for overnight. The reaction mixture was concentrated in vacuo. The residue was acidified with 2H HCl solution and extracted into diethyl ether. The organic layer was washed with brine, dried over MgSO4, filtrated and the solvent was evaporated. The residue was washed with diisopropyl ether to give the title compound 12.1 g as brown solid. Yield 84.5%
(4) 3-Methoxy-4-(methoxymethoxy)-2-nitrobenzonitrile




A mixture of 4-hydroxy-3-methoxy-2-nitrobenzonitrile 1.00 g, chloromethyl methyl ether 0.47 mL (6.18 mmol) and potassium carbonate 3.56 g (25.8 mmol) in N,N-dimethylformamide 10 mL was stirred at 50° C. for 2 hours. The reaction mixture was poured into water and extracted into diethyl ether. The organic layer was washed with brine, dried over MgSO4, filtrated and the solvent was evaporated. Silica gel chromatography (n-hexane/AcOEt=4/1) afforded the title compound 1.03 g as colorless solid. Yield 83.5%.
(5) 2-Amino-3-methoxy-4-(methoxymethoxy)benzonitrile




To 5% palladium on activated carbon 6.00 g under argon atmosphere was added a solution of 3-methoxy-4-(methoxymethoxy)-2-nitrobenzonitrile 6.00 g (25.2 mmol) in ethanol 50 mL and stirred under hydrogen atmosphere at room temperature for 8 hours. The reaction mixture was filtrated and the filtrate was concentrated in vacuo. Silica gel chromatography (n-hexane/AcOEt=4/1) afforded the title compound 2.83 g as white solid. Yield 53.9%.
(6) [6-(4,5-Dihydro-1H-imidazol-2-yl)-2-methoxy-3-(methoxymethoxy)phenyl]amine




A solution of 2-amino-3-methoxy-4-(methoxymethoxy)benzonitrile 475 mg (2.28 mmol) and phosphorus pentasulfide 25.4 mg (0.11 mmol) in ethylenediamine 2.75 g was stirred at 120° C. for overnight. The reaction mixture was cooled to room temperature and poured into water. The precipitate was collected and washed with water to give the title compound 293 mg as white solid. Yield 51.1%.
(7) Ethyl 3-oxo-3-(pyridin-3-yl)propanoate




To a suspension of nicotinic acid 5.00 g (40.6 mmol) in tetrahydrofuran 50 mL was added carbonyl diimidazole 9.76 g (60.9 mmol) at 5° C. and stirred at room temperature for 1 hour. In a separate flask, a suspension of MgCl2 4.64 g (48.7 mmol) and ethyl malonate potassium salt 10.37 g (60.92 mmol) in tetrahydrofuran 50 mL was stirred at 50° C. for 4 hours. To this suspension was added the aforementioned imidazolide solution at room temperature and stirred for 12 hours. The reaction was quenched by the addition of water and extracted into ethyl acetate. The organic layer was washed by brine, dried over MgSO4, filtrated and the solvent was evaporated. Silica gel chromatography (n-hexane/AcOEt=2/1) afforded the titla compound 3.89 g as pale yellow oil. Yield 49.5%.
(8) 2-[7-Methoxy-8-(methoxymethoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-1-pyridin-3-ylethylenol




A solution of [6-(4,5-dihydro-1H-imidazol-2-yl)-2-methoxy-3-(methoxymethoxy)phenyl]amine 1.31 g (5.20 mmol) and ethyl 3-oxo-3-(pyridin-3-yl)propanoate 1.00 g (5.20 mmol) in toluene 30 mL was refluxed for overnight. The precipitate was collected and washed with diethyl ether to give the title compound 1.52 g as a yellow solid. Yield 76.9%.
Melting point: 215-216° C. Mass spectrometry: 381 In vitro PI3K-β inhibitory activity: In vitro PI3K-γ inhibitory activity: B H-NMR (500 MHz, CDCl3) δ: 3.54 (s 3H), 3.95 (t 2H J=9.5 Hz), 4.08 (s 3H), 4.22 (t 2H J=9.5 Hz), 5.30 (s 2H), 5.38 (s 1H), 6.98 (d 1H J=8.8 Hz), 7.37 (dd 1H J=8.0 Hz, 4.9 Hz), 7.64 (d 1H J=8.8 Hz), 8.21 (dt 1H J=8.0 Hz, 1.7 Hz), 8.67 (dd 1H J=4.9 Hz, 1.7 Hz), 9.09 (d 1H J=1.7 Hz), 13.75 (s 1H)
Example 1-4
5-(2-Hydroxy-2-pyridin-3-ylvinyl)-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-8-ol hydrochloride




A suspension of 2-[7-methoxy-8-(methoxymethoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-1-pyridin-3-ylethylenol (Example 1-3) 1.52 g (4.00 mmol) in 4N HCl in 1,4-dioxane 30 mL and water 0.3 mL was stirred at room temperature for overnight. The reaction mixture was diluted with diethyl ether. The precipitate was collected and washed with diethyl ether to give the title compound 1.23 g as a yellow solid. Yield 82.4%
Melting point: 245° C. Mass spectrometry: 337 In vitro PI3K-β inhibitory activity: C In vitro PI3K-γ inhibitory activity: A H-NMR (500 MHz, DMSO-d6) δ: 3.97 (s 3H), 4.22 (dd 2H J=12.3 Hz, 9.0 Hz), 4.43 (dd 2H J=12.3 Hz, J=9.0 Hz), 6.17 (s 1H), 7.10 (d 1H J=9.0 Hz), 7.71(dd 1H J=7.7 Hz, 4.7 Hz), 7.98 (d 1H J=9.0 Hz), 8.57 (br d 1H J=7.7 Hz), 8.82 (dd 1H J=4.7 Hz, 1.4 Hz), 9.34 (d 1H J=1.4 Hz), 11.79 (s 1H), 14.60 (s 1H)
Example 1-5
Methyl 4-{[5-(2-hydroxy-2-pyridin-3-ylvinyl)-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-8-yl]oxy}butanoate




A mixture of 5-(2-hydroxy-2-pyridin-3-ylvinyl)-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-8-ol hydrochloride (Example 1-4) 50.4 mg (0.14 mmol), methyl chloro-butyrate 22.2 mg (0.16 mmmol) and potassium carbonate 186.9 mg (1.35 mmmol) in N,N-dimethylformamide 1 mL was stirred at 120° C. for 4 hours. The reaction mixture was poured into water and extracted into dichloromethane. The organic layer was washed with brine, dried over MgSO4, filtrated and the solvent was evaporated. The residue was washed by diethyl ether to give the title compound 35.0 mg as yellow solid. Yield 59.3%.
Melting point: 199-200° C. Mass spectrometry: 437 In vitro PI3K-β inhibitory activity: C In vitro PI3K-γ inhibitory activity: A H-NMR (500 MHz, CDCl3) δ: 2.20 (quint 2H J=7.1 Hz), 2.58 (t 2H J=7.09 Hz), 3.71 (s 3H), 3.94 (t 2H J=9.5 Hz), 4.06 (s 3H), 4.15 (t 2H J=7.1 Hz), 4.21 (t 2H J=9.5 Hz), 5.38 (s 1H), 6.76 (d 1H J=8.8 Hz), 7.37 (dd 1H J=8.2 Hz, 5.2 Hz), 7.65 (d 1H J=8.8 Hz), 8.21 (dt J=8.2 Hz, 2.1 Hz), 8.67 (d 1H J=5.2 Hz), 9.09 (s 1H), 13.70 (s 1H)
Example 1-6
Example 3-4
4-{[5-(2-Hydroxy-2-pyridin-3-ylvinyl)-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-8-yl]oxy}butanoic acid




A solution of methyl 4-{[5-(2-hydroxy-2-pyridin-3-ylvinyl)-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-8-yl]oxy}butanoate (example 1-5) 20.0 mg (0.05 mmol) in 1N LiOH solution 0.1 mL and ethanol 1.0 mL was stirred at room temperature for overnight. The reaction mixture was neutralized with 1N HCl solution and concentrated in vacuo. The residue was triturated in water. The precipitate was collected to give the title compound 10.0 mg as white solid. Yield 51.7%.
Melting point: 257-258° C. Mass spectrometry: 423 In vitro PI3K-β inhibitory activity: B In vitro PI3K-γ inhibitory activity: A H-NMR (500 MHz, DMSO-d6) δ: 2.02 (quint 2H J=6.2 Hz), 2.45 (t 2H J=6.2 Hz), 3.94 (s 3H), 3.98 (br t 2H J=8.5 Hz), 4.06 (br t 2H J=8.5 Hz), 4.14 (t 2H J=6.2 Hz), 5.67 (s 1H), 6.97 (d 1H J=8.7 Hz), 7.49 (dd 1H J=8.2 Hz, 4.4 Hz), 7.57 (d 1H J=8.7 Hz), 8.29 (d 1H J=8.2 Hz), 8.67 (d 1H J=4.4 Hz), 9.14 (s 1H), 12.15 (s 1H), 13.76 (s 1H)
Example 1-7
4-{[5-(2-Hydroxy-2-pyridin-3-ylvinyl)-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-8-yl]oxy}butanoic acid hydrochloride




A mixture of 4-{[5-(2-hydroxy-2-pyridin-3-ylvinyl)-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-8-yl]oxy}butanoic acid (Example 1-6) 4.0 mg (9.5 micromol) in 4N HCl in 1,4-dioxane 2.0 mL was stirred at room temperature for 2 hours. The reaction mixture was diluted with diethyl ether. The precipitate was collected to give the title compound 4.00 mg as a yellow solid. Yield 92.0%.
Melting point: 249-251° C. Mass spectrometry: 423 In vitro PI3K-β inhibitory activity: B In vitro PI3K-γ inhibitory activity: A H-NMR (500 MHz, DMSO-d6) δ: 2.06 (quint 2H J=7.3 Hz), 2.46 (t 2H J=7.3 Hz), 4.01 (s 3H), 4.24 (t 2H J=9.0 Hz), 4.29 (t 2H J=7.3 Hz), 4.45 (t 2H J=9.0 Hz), 6.18 (s 1H), 7.36 (d 1H J=9.1 Hz), 7.70 (dd 1H J=7.9 Hz, 5.0 Hz), 8.14 (d 1H J=9.1 Hz), 8.56 (br d 1H J=7.9 Hz), 8.82 (br d 1H J=5.0 Hz), 9.34 (s 1H), 12.34 (s 1H), 14.57 (s 1H)
Example 1-8
2-[7-Methoxy-8-(4-morpholin-4-yl-4-oxobutoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-1-pyridin-3-ylethylenol




To a solution of 4-{[5-(2-hydroxy-2-pyridin-3-ylvinyl)-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-8-yl]oxy}butanoic acid (Example 1-6) 20.0 mg (0.044 mmol), morpholine 19.0 mg (0.22 mmol) and N,N-diisopropylethylamine 0.038 mL (0.22 mmol) in N,N-dimethylformamide 2.0 mL was added PyBOP((1H-1,2,3-benzotriazol-1-yloxy)(tripyrrolidin-1-yl)phosphonium hexafluorophosphate) 34.0 mg (0.065 mmol) and stirred at 80° C. for overnight. After cooling to room temperature, the reaction mixture was poured into water. The precipitate was collected and washed with water to give the title compound 13.0 mg as a white solid. Yield 60.7%.
Melting point: 234-235° C. Mass spectrometry: 492 In vitro PI3K-β inhibitory activity: B In vitro PI3K-γ inhibitory activity: A H-NMR (500 MHz, DMSO-d6) δ:2.03 (quint 2H J=6.6 Hz), 3.46 (m 4H), 3.56 (m 4H), 3.96 (s 3H), 3.99 (br d 2H J=8.2 Hz), 4.05 (br d 2H J=8.2 Hz), 4.15 (t 2H J=6.6 Hz), 5.66 (s 1H), 6.98 (d J=8.8 Hz), 7.50 (dd 1H J=7.7 Hz, 4.7 Hz), 7.57 (d 1H J=8.8 Hz), 8.29 (br d 1H J=7.7 Hz), 8.67 (br d 1H J=4.7 Hz), 9.14 (s 1H), 13.76 (s 1H)
In a similar method according to the Example 1-1 to 1-8 above, the compounds in Example 1-9 to 1-210 were synthesized.












TABLE 1







Mol
MS




Ex. No.
Structure
Weight
(M + 1)
mp
in vitro




















1-9




372.81
337
245 (dec.)
A


 


1-10




350.38
351
269-270
A


 


1-11




386.84
351
249-250
A


 


1-12




407.43
408
270 (dec.)
A


 


1-13




364.41
365
267-268
A


 


1-14




378.43
379
252-253
A


 


1-15




390.45
391
254 (dec.)
B


 


1-16




380.41
381
264-265
A


 


1-17




416.87
381
215 (dec.)
A


 


1-18




450.50
451
184-186
B


 


1-19




407.48
408
183-184
B


 


1-20




447.54
448
162-163
B


 


1-21




433.51
434
204-205
A


 


1-22




430.85
395
240 (dec.)
A


 


1-23




393.41
394
297-298
A


 


1-24




429.87
394
235 (dec.)
A


 


1-25




443.89
408
240 (dec.)
A


 


1-26




471.95
436
245 (dec.)
A


 


1-27




421.46
422
241-242
A


 


1-28




457.92
422
205 (dec.)
A


 


1-29




463.50
464
235-235
A


 


1-30




499.96
464
240-241
A


 


1-31




537.98
502
230-231
B


 


1-32




391.43
392
>285 
A


 


1-33




427.89
392
273
A


 


1-34




373.42
374
>285 
A


 


1-35




409.88
374
270
A


 


1-36




449.51
450
197
A


 


1-37




485.97
450
215
A


 


1-38




543.03
507
260
A


 


1-39




433.51
434
217
B


 


1-40




469.98
434
256 (dec.)
B


 


1-41




527.03
491
271
A


 


1-42




350.38
351
218
A


 


1-43




386.84
351
290 (dec.)
A


 


1-44




476.76
442,440
>290 
B


 


1-45




419.71
385,383
>290 
B


 


1-46




476.76
442,440
>285 
A


 


1-47




442.29
424,422
>285 
B


 


1-48




458.75
424,422
>285 
B


 


1-49




364.41
365
200-204
A


 


1-50




400.87
365
260 (dec.)
B


 


1-51




443.89
408
275-280
B


 


1-52




379.42
380
321-325
B


 


1-53




393.45
394
195..198
B


 


1-54




409.45
410
207
B


 


1-55




384.83
385
283
B


 


1-56




389.42
390
212-215
A


 


1-57




425.88
390
240 (dec.)
A


 


1-58




355.42
356
250
B


 


1-59




391.88
356
266-268
B


 


1-60




384.46
385
292
A


 


1-61




420.92
385
268-271
A


 


1-62




364.41
365
278
A


 


1-63




400.87
365
285
A


 


1-64




421.46
422
>285 
A


 


1-65




457.92
422
>285
A


 


1-66




403.44
404
280
B


 


1-67




439.91
404
>285 
B


 


1-68




320.35
321
275
A


 


1-69




356.81
321
285
A


 


1-70




308.32
309
218
A


 


1-71




344.78
309
303
B


 


1-72




324.77
325
210 (dec.)
B


 


1-73




369.22
371,369
120 (dec.)
B


 


1-74




405.68
371,369
246
B


 


1-75




304.35
305
248
B


 


1-76




340.82
305
>290 
B


 


1-77




361.41
362
>285 
A


 


1-78




397.87
362
>285 
A


 


1-79




379.85
344
>285 
A


 


1-80




358.33
359
275
B


 


1-81




394.79
359
>290 
B


 


1-82




389.46
390
198-202(dec.)
B


 


1-83




342.79
307
>250
B


 


1-84




419.49
420
195-196
B


 


1-85




455.95
420
261-262
B


 


1-86




377.45
378
186-187
B


 


1-87




391.48
392
235 (dec.)
B


 


1-88




360.42
361
203 (dec.)
B


 


1-89




396.88
361
>300 
B


 


1-90




420.47
421
222-223
A


 


1-91




350.38
351
211-212
B


 


1-92




364.41
365
203-205
A


 


1-93




348.36
349
225-226
B


 


1-94




375.43
376
282
B


 


1-95




411.89
376
>300 
B


 


1-96




432.49
433
269 (dec.)
A


 


1-97




468.95
433
246
A


 


1-98




391.43
392
337 (dec.)
A


 


1-99




427.89
392
312 (dec.)
A


 


1-100




414.47
415
232
A


 


1-101




450.93
415
286 (dec.)
A


 


1-102




482.97
447
238 (dec.)
B


 


1-103




501.04
466
257
B


 


1-104




424.94
389
288
B


 


1-105




445.53
446
292 (dec.)
B


 


1-106




481.99
446
280 (dec.)
B


 


1-107




427.51
428
207
A


 


1-108




463.97
428
>300 
B


 


1-109




416.49
416

A


 


1-110




438.92
403
231 (dec.)
B


 


1-111




389.46
390
204
B


 


1-112




425.92
390
242
B


 


1-113




446.51
447
245
B


 


1-114




482.97
447
260
B


 


1-115




428.50
429
219
B


 


1-116




324.77
325
226
B


 


1-117




361.23
326
280 (dec.)
B


 


1-118




405.68
371,369
233
B


 


1-119




304.35
305
224
B


 


1-120




340.82
305
>330 
B


 


1-121




358.33
359
264
C


 


1-122




394.79
359
321
B


 


1-123




402.89
>300 
B


 


1-124




306.33
307
302-303
B


 


1-125




342.79
307
>300 
A


 


1-126




320.35
321
199
B


 


1-127




356.81
321
>300 
B


 


1-128




399.84
364
>300 
A


 


1-129




405.68
371,369
>300
B


 


1-130




361.23
326
>330 
B


 


1-131




304.35
305
212
B


 


1-132




340.82
305
>290 
B


 


1-133




346.39
347
>300 
B


 


1-134




290.33
291
202
B


 


1-135




326.79
291
260 (dec.)
B


 


1-136




304.35
305
217-219
B


 


1-137




340.82
305
>300 
B


 


1-138




383.84
348
327
A


 


1-139




319.37
320
232-237
A


 


1-140




347.42
348
197
B


 


1-141




291.31
292
233-235
B


 


1-142




327.78
292
217-222
B


 


1-143




279.30
280
192
B


 


1-144




315.76
280
>300 
B


 


1-145




279.30
280
155-156
B


 


1-146




295.37
296
193
A


 


1-147




381.83
296
>300 
A


 


1-148




295.37
296
182-183
B


 


1-149




331.83
296
>300 
A


 


1-150




278.32
279
247
B


 


1-151




278.32
279
247-249
A


 


1-152




280.29
281
148
B


 


1-153




316.75
281
245 (dec.)
B


 


1-154




296.35
297
208-210
A


 


1-155




332.81
297
>300 
B


 


1-156




324.41
325
222
A


 


1-157




365.83
330
>300 
B


 


1-158




330.60
330
190 (dec.)
B


 


1-159




330.35
331
>300 
A


 


1-160




366.81
331
247 (dec.)
B


 


1-161




362.39
363
>300 
B


 


1-162




399.84
400
>300 
B


 


1-163




419.49
420
200
B


 


1-164




291.31
292
230
B


 


1-165




291.31
292
250
B


 


1-166




289.34
290
130-139
C


 


1-167




334.34
335
276
D


 


1-168




334.34
335
240-248
D


 


1-169




319.37
320
212-214
D


 


1-170




305.34
306
252-256
D


 


1-171




323.78
324
224-227
D


 


1-172




315.35
315
260-264
D


 


1-173




290.33
291
195
C


 


1-174




326.79
291
235-240
C


 


1-175




290.33
291
204-205
B


 


1-176




326.79
291
235 (dec.)
B


 


1-177




320.38
321
256
C


 


1-178




340.36
341
255-258
D


 


1-179




425.51
426
>300 
D


 


1-180




345.43
346
220-225
D


 


1-181




381.89
346
>300 
D


 


1-182




255.32
256
113
D


 


1-183




269.35
270
134-138
C


 


1-184




281.24
282
240
C


 


1-185




349.39
350
249-252
C


 


1-186




383.84
384
257-259
D


 


1-187




374.40
375
307-308
D


 


1-188




358.33
359
264
C


 


1-189




324.77
325
260
C


 


1-190




323.78
324
186-188
C


 


1-191




334.34
335
259-262
D


 


1-192




335.32
336
306
C


 


1-193




317.39
318
156-160
D


 


1-194




434.50
435
233-234
A


 


1-195




375.39
376
284-285
A


 


1-196




418.42
419
229-231
A


 


1-197




454.88
419
217-218
A


 


1-198




528.01
492
215-216
A


 


1-199




436.47
437
178-179
A


 


1-200




430.85
395
286 (dec.)
B


 


1-201




398.85
363
273 (dec.)
A


 


1-202




413.87
378
285 (dec.)
B


 


1-203




405.46
406
228
B


 


1-204




447.50
448
262
C


 


1-205




445.53
446
246
B


 


1-206




427.89
392
267
A


 


1-207




425.92
390
259 (dec.)
B


 


1-208




446.51
447
253 (dec.)
B


 


1-209




482.97
447
>260 
B


 


1-210




464.96
429
>300 
A









Example 2-1
N-(2,3-Dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide
(1) 2-(4,5-Dihydro-1H-imidazol-2-yl)aniline




2-Aminobenzonitrile (9.00 g, 76.2 mmol) was added at 0° C. to ethylenediamine (25.5 ml, 381 mmol) in small portions with stirring. After phosphorus pentasulfide (200 mg, 0.900 mmol) was added, the mixture was stirred at 100° C. overnight. After cooling to 0° C., the reaction was diluted with water. The resulting white precipitate was collected by filtration, washed with water and diethyl ether, and dried under reduced pressure to give 2-(4,5-dihydro-1H-imidazol-2-yl)aniline (10.0 g, 81% yield).
(2) 2,3-Dihydroimidazo[1,2-c]quinazolin-5-ylamine hydrobromide




To a suspension of 2-(4,5-dihydro-1H-imidazol-2-yl)aniline (5.00 g, 31.0 mmol) in 85% methanol (60 ml) at 0° C. was added cyanogen bromide (3.61 g, 34.1 mmol) by portions. This mixture was stirred at room temperature overnight. After the mixture was concentrated under reduced pressure, the resulting precipitate was collected by filtration. This pale green solid was washed with water, methanol and diethyl ether successively, and dried under reduced pressure to give 2,3-dihydroimidazo[1,2-c]quinazolin-5-ylamine hydrobromide (4.94 g, 60% yield).
(3) N-(2,3-Dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide




To a suspension of 2,3-dihydroimidazo[1,2-c]quinazolin-5-ylamine hydrobromide (500 mg, 1.87 mmol) and nicotinic acid (346 mg, 2.81 mmol) in N,N-dimethyl-formamide (25 ml) at room temperature was added benzotriazole-1-yl-oxy-tris-pyrrolidino-phosphonium hexafluorophosphate (1.46 g, 2.81 mmol) followed by N,N-diisopropylethylamine (1.30 ml, 7.49 mmol). The mixture was heated at 80° C. for 4 hours. After cooling to room temperature, the mixture was quenched with aqueous saturated NaHCO3 solution. The resulting precipitate was collected by filtration, washed with water and diethyl ether, and dried under reduced pressure to give N-(2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide (450 mg, 83% yield).
Melting point: 238-239° C. (decomposition) Mass spectrometry: 292 In vitro PI3K-β inhibitory activity: B In vitro PI3K-γ inhibitory activity: A 1H-NMR (300 MHz, DMSO-d6): d 4.00-4.11 (2H, m), 4.11-4.21 (2H, m), 7.29 (1H, ddd, J=3.0, 5.3, 7.9 Hz), 7.52 (1H, dd, J=4.9, 7.9 Hz), 7.57-7.66 (2H, m), 7.89 (1H, d, J=7.9 Hz), 8.42-8.48 (1H, m), 8.73 (1H, dd, J=1.9, 4.9 Hz) 9.32 (1H, d, J=1.1 Hz), 12.36 (1H, s).
Example 2-2
N-(2,3-Dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide hydrochloride




To a suspension of N-(2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide (150 mg, 0.515 mmol) in tetrahydrofuran (4 ml) at 0° C. was added a 4N solution of hydrochloric acid in 1,4-dioxane (2 ml, 8 mmol). The mixture was stirred at room temperature for 1 h, and concentrated under reduced pressure. The resulting residue was triturated with diethyl ether. The resulting precipitate was collected by filtration, washed with ethyl ether, and dried under reduced pressure to give N-(2,3-dihydro-imidazo[1,2-c]quinazolin-5-yl)nicotinamide hydrochloride (192 mg, quantitative).
Melting point: 289° C. (decomposition) Mass spectrometry: 292 In vitro PI3K-β inhibitory activity: B In vitro PI3K-γ inhibitory activity: A 1H-NMR (300 MHz, DMSO-d6): δ 4.18-4.30 (2H, m), 4.54-4.65 (2H, m), 7.56-7.65 (1H, m), 7.88 (1H, dd, J=4.9, 7.9 Hz), 7.97-8.10 (2H, m), 8.64 (1H, d, J=7.9 Hz), 8.80 (1H, d, J=7.9 Hz), 8.95 (1H, dd, J=1.5, 5.3 Hz), 9.43 (1H, d, J=1.1 Hz), 12.7-13.3 (1H, br).
Example 2-3
6-(Acetamido)-N-[8-(morpholin-4-yl)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide
(1) 4-(Morpholin-4-yl)-2-nitrobenzonitrile




A mixture of 2,4-dinitrobenzonitrile 4.20 g (21.75 mmol) and morpholine 5.7 mL (66.0 mmol) in N,N-dimethyformamide 20 mL was stirred at room temperature for 20 hours. The reaction mixture was poured into water. The precipitate was collected and washed with water to give the title compound 4.20 g as orange solid. Yield 74.5%.
(2) 2-Amino-4-(morpholin-4-yl)benzonitrile




To a cooled mixture of tin(II) chloride dihydrate 12.8 g (56.7 mmol) in conc. HCl 40 mL with ice bath was added 4-(morpholin-4-yl)-2-nitrobenzonitrile 4.20 g (16.09 mmol) and stirred at room temperature for 2 hours. The reaction mixture was poured into diluted NaOH solution and extracted into ethyl acetate. The organic layer was washed with water and brine, dried over MgSO4 and the solvent was evaporated. The crude product was washed with diethyl ether to give the title compound 3.13 g as off-white solid. Yield 95.0%.
(3) [2-(4,5-dihydro-1H-imidazol-2-yl)-5-(morpholin-4-yl)phenyl]amine




To a solution of 2-amino-4-(morpholin-4-yl)benzonitrile 3.65 g (18.0 mmol) in ethylenediamine 20 mL was added phosphorus pentasulfide 4.00 mg (0.018 mmol) and stirred at 140° C. for 16 hours. After cooling to room temperature, the solvent was evaporated. The residue was washed with water and diethyl ether to give the title compound 3.70 g as off-white solid. Yield 83.5%.
(4) 8-(Morpholin-4-yl)-2,3-dihydroimidazo[1,2-c]quinazolin-5-amine hydrobromide




To a suspension of [2-(4,5-dihydro-1H-imidazol-2-yl)-5-(morpholin-4-yl)phenyl]amine 3.60 g (14.6 mmol) in 2-propanol 20 mL was added cyanogen bromide 2.32 g (21.9mmol) portionwise at 0° C. and stirred at 100° C. for 2 hours. After cooling to room temperature, the precipitate was collected and washed with diethyl ether to give the title compound 1.20 g as yellow solid. Yield 77.5%.
(5) 6-(Acetamido)nicotinic acid




A mixture of 6-aminonicotinic acid 5.00 g (36.5 mmol) and acetic anhydride 3.80 mL (40.2 mmol) in pyridine 30 mL was stirred at 140° C. for 24 hours. To the reaction mixture was added ethyl acetate and acidified with diluted HCl solution to pH 2. The organic layer was washed with water and brine, dried over MgSO4, filtrated and the solvent was evaporated. The residue was washed with diisopropyl ether to give the title compound 1.70 g as off-white solid. Yield 26%.
(6) 6-(Acetamido)-N-[8-(morpholin-4-yl)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide




To a mixture of 8-(morpholin-4-yl)-2,3-dihydroimidazo[1,2-c]quinazolin-5-amine hydrobromide 105.7 mg (0.30 mmol), 6-(acetamido)nicotinic acid 81.1 mg (0.45 mmol) and N,N-diisopropylethylamine 0.26 mL (1.50 mmol) in N,N-dimethylformamide 2 mL was added PyBOP((1H-1,2,3-benzotriazol-1-yloxy)(tripyrrolidin-1-yl)-phosphonium hexafluorophosphate) 234.2 mg (0.45 mmol) and stirred at 90° C. for 16 hours. After cooling to room temperature, saturated NaHCO3 solution was added. The precipitate was collected and washed with water, methanol, and diethyl ether to give the title compound 41.1 mg as yellow solid. Yield 31.6%.
Melting point: 228° C. Mass spectrometry: 434 In vitro PI3Kβ inhibitory activity: C In vitro PI3K-γ inhibitory activity: A H-NMR (500 MHz, DMSO-d6) δ: 3.22-3.30 (m 4H), 3.74 (s 3H), 3.86 (m 2H), 3.97 (m 2H), 6.77 (br s 1H), 7.60 (m 1H), 8.07 (m 1H), 8.32 (m 1H), 8.95 (br s 1H), 10.60 (s 1H)
Example 2-4
6-(Acetamido)-N-[8-(morpholin-4-yl)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide hydrochloride




To a mixture of 6-(acetamido)-N-[8-(morpholin-4-yl)-2,3-dihydroimidazo[1,2-c]-quinazolin-5-yl]nicotinamide (Example 2-3) 20.0 mg (0.046 mmol) in 1,4-dioxane 1.5 mL was added 4N HCl in 1,4-dioxane 0.5 mL and stirred at room temperature for 40 minutes. The precipitate was collected and washed with diethyl ether to give the title compound 17.0 mg as yellow solid. Yield 78%.
Melting point: 237° C. Mass spectrometry: 434 In vitro PI3K-β inhibitory activity: B In vitro PI3K-γ inhibitory activity: A H-NMR (500 MHz, DMSO-d6) δ: 3.41-3.76 (m 7H), 3.86 (m 2H), 4.10 (m 2H), 7.20 (m 1H), 7.39 (m 1H), 8.19 (m 1H), 8.45 (m 1H), 9.09 (br s 1H), 10.86 (s 1H)
Example 2-5
N-(8-Hydroxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide




A suspension of N-(8-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide (example 2-22) 3.50 g (10.9 mmol) and sodium sulfide 4.25 g (54.5 mmol) in 1-methyl-2-pyrrolidinone 10 mL was heated to 160° C. for 4 hours (LC-MS indicated complete consumption of the starting material). The mixture was cooled to room temperature and volatile sideproducts were evaporated. The mixture was partitioned between chloroform and 0.5N NaOH solution. The aqueous layer was neutralized and the formed precipitate was collected to give the title compound 2.34 g as off-white solid. Yield 69.9%.
Melting point: 289° C. Mass spectrometry: 308 In vitro PI3K-β inhibitory activity: C In vitro PI3K-γ inhibitory activity: B H-NMR (500 MHz, DMSO-d6) δ: 4.01 (m 2H), 4.15 (m 2H), 6.75 (dd 1H J=8 Hz, 2 Hz), 6.91 (s 1H), 7.52 (dd 1H J=8 Hz, 5 Hz), 7.75 (d 1H J=8 Hz), 8.44 (d 1H J=8 Hz), 8.73 (dd 1H J=5 Hz, 2 Hz), 9.31 (s 1H), 10.61 (br s 1H), 12.24 (br s 1H)
Example 2-6
N-{8-[2-(1-pyrrolyl)ethoxy]-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl}nicotinamide




The suspension of N-(8-Hydroxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide (example 2-1) 70.0 mg (0.23 mmol), N-(2-bromoethyl)pyrrole 47.6 mg (0.27 mmol) and potassium carbonate 126 mg (0.91 mmol) in N,N-dimethylformamide 5 mL was heated in a sealed tube to 120° C. for 3 hours. The reaction mixture was concentrated and partitioned between dichloromethane and water. The organic layer was washed with 0.1N NaOH solution and brine, dried over Na2SO4 and the solvent was evaporated to give the title compound 49.0 mg as off-white solid. Yield 54%.
Melting point: 209° C. Mass spectrometry: 401 In vitro PI3Kβ inhibitory activity: B In vitro PI3K-γ inhibitory activity: B H-NMR (500 MHz, DMSO-d6) δ: 4.00 (m 2H), 4.12 (m 2H), 4.30 (s 4H), 6.00 (m 2H), 6.84 (m 2H), 6.85 (dd 1H J=6 Hz, 2 Hz), 7.27 (d 1H J=2 Hz), 7.52 (dd 1H J=6 Hz), 7.76 (d 1H J=8Hz), 8.44 (dd 1H J=8 Hz, 2 Hz), 8.72 (dd 1H J=5 Hz, 2 Hz), 9.31 (s 1H), 12.32 (s 1H)
In a similar method according to the Example 2-1 to 2-6 above, the compounds in Example 2-7 to 2-368 were synthesized.












TABLE 2










in vitro


Ex.




PI3K-


No.
Structure
MW
MASS
mp/° C.
gamma




















2-7




376.42
377
243
B


 


2-8




412.88
377
283
A


 


2-9




468.95
433
249
B


 


2-10




415.46
416
250 (dec.)
B


 


2-11




451.92
416
294 (dec.)
A


 


2-12




390.45
391
199 (dec.)
B


 


2-13




390.45
391
209
A


 


2-14




426.91
391
267 (dec.)
A


 


2-15




432.49
433
227
B


 


2-16




410.50
411
233 (dec.)
B


 


2-17




446.96
411
255 (dec.)
A


 


2-18




407.48
408
232
B


 


2-19




410.91
376
>300
B


 


2-20




321.34
322
281 (dec.)
B


 


2-21




357.80
322
292 (dec.)
B


 


2-22




414.85
379
198-205 (dec.)
B


 


2-23




336.36
337
279-282
A


 


2-24




372.82
337
273 (dec.)
A


 


2-25




360.38
361
186
A


 


2-26




396.84
361
233
A


 


2-27




305.34
306
207
A


 


2-28




341.80
306
315
A


 


2-29




344.38
345
190
A


 


2-30




380.84
345
295
B


 


2-31




310.38
311
182
B


 


2-32




346.84
311
276
B


 


2-33




359.31
360
229
B


 


2-34




395.77
360
275
A


 


2-35




411.77
375
237 (dec.)
A


 


2-36




398.35
399
>300
B


 


2-37




434.81
399
288
A


 


2-38




362.22
327
308
B


 


2-39




364.80
366
288
A


 


2-40




401.26
366
270
A


 


2-41




367.26
332
328
B


 


2-42




406.67
372,370
243
A


 


2-43




420.70
386,384
252 (dec.)
B


 


2-44




409.25
411,409
262
B


 


2-45




445.71
411,409
278
A


 


2-46




351.37
352
259-260
A


 


2-47




387.83
352
257-257
A


 


2-48




408.42
409
306-307
A


 


2-49




390.40
391
289 (dec.)
A


 


2-50




426.87
391
278 (dec.)
A


 


2-51




391.39
392
233 (dec.)
A


 


2-52




427.85
392
210 (dec.)
A


 


2-53




387.83
352
246
B


 


2-54




367.37
367
287 (dec.)
A


 


2-55




403.83
367
260 (dec.)
A


 


2-56




402.84
367
256
B


 


2-57




408.42
409
224
B


 


2-58




444.88
409
279
B


 


2-59




401.86
366
257 (dec.)
B


 


2-60




390.40
391
246
A


 


2-61




426.87
391
276
A


 


2-62




356.41
357
248
B


 


2-63




376.81
340
270 (dec.)
B


 


2-64




368.40
368
236-237
B


 


2-65




400.24
402,400
264
A


 


2-66




436.70
402,400
298
A


 


2-67




436.70
402,400
289 (dec.)
B


 


2-68




351.37
352
228 (dec.)
A


 


2-69




387.83
352
275 (dec.)
B


 


2-70




408.42
408
286 (dec.)
B


 


2-71




444.88
408
270 (dec.)
B


 


2-72




390.40
391
210 (dec.)
A


 


2-73




426.87
391
289 (dec.)
A


 


2-74




420.70
386,384
220
A


 


2-75




423.28
425,423
>290
B


 


2-76




401.86
366
235 (dec.)
B


 


2-77




379.42
379
210 (dec.)
A


 


2-78




415.88
379
230 (dec.)
A


 


2-79




422.45
422
>310
B


 


2-80




458.91
422
305 (dec.)
A


 


2-81




404.43
405
202
B


 


2-82




440.89
405
280 (dec.)
B


 


2-83




384.80
349
>300
B


 


2-84




325.76
326
210
B


 


2-85




362.22
327
309
B


 


2-86




401.26
366
305 (dec.)
B


 


2-87




370.21
372
228
B


 


2-88




406.67
372,370
316
B


 


2-89




445.71
411,409
288
B


 


2-90




305.34
306
210
A


 


2-91




341.80
306
>290
B


 


2-92




380.84
345
>290
A


 


2-93




357.80
322
>300
B


 


2-94




396.84
361
288
A


 


2-95




317.35
318
196-198
B


 


2-96




353.81
318
275-277
B


 


2-97




393.84
358
298-299
B


 


2-98




362.22
327
249
B


 


2-99




309.31
310
243
B


 


2-100




345.77
310
288
A


 


2-101




348.34
349
>300
A


 


2-102




384.80
349
>300
A


 


2-103




362.22
326
>280
B


 


2-104




382.81
383
>280
B


 


2-105




419.27
383
>280
A


 


2-106




401.26
365
>280
B


 


2-107




305.34
306
244
B


 


2-108




341.80
306
>290
B


 


2-109




344.38
345
>290
A


 


2-110




380.84
345
>290
A


 


2-111




395.77
360
263
A


 


2-112




398.35
399
286
A


 


2-113




434.81
399
270
A


 


2-114




321.34
322
110
A


 


2-115




357.80
322
237 (dec.)
A


 


2-116




335.37
335
204-205
B


 


2-117




371.83
335
251 (dec.)
A


 


2-118




355.79
355
185 (dec.)
A


 


2-119




392.25
355
266 (dec.)
A


 


2-120




371.83
335
220 (dec.)
A


 


2-121




389.34
389
144-145
B


 


2-122




373.80
338
285 (dec.)
A


 


2-123




372.82
337
296
A


 


2-124




360.38
361
287
A


 


2-125




396.84
361
238
A


 


2-126




386.42
386
183-184
A


 


2-127




422.88
386
225 (dec.)
A


 


2-128




440.39
440
214 (dec.)
A


 


2-129




476.85
440
225 (dec.)
A


 


2-130




405.34
292
237-239
A


 


2-131




305.34
306
193-194
B


 


2-132




341.80
306
277 (dec.)
B


 


2-133




306.33
306
215 (dec.)
B


 


2-134




325.76
326
198-199
A


 


2-135




362.22
326
340 (dec.)
B


 


2-136




305.34
305
194-195
B


 


2-137




341.80
305
291 (dec.)
B


 


2-138




307.31
307
273 (dec.)
A


 


2-139




343.78
307
296-297
A


 


2-140




321.34
321
219 (dec.)
B


 


2-141




357.80
321
272 (dec.)
B


 


2-142




335.32
336
358-359
B


 


2-143




384.42
385
265-269
A


 


2-144




306.33
307
263-266
A


 


2-145




420.35
307
220 (dec.)
B


 


2-146




361.41
362
219 (dec.)
B


 


2-147




305.34
306
195-196
A


 


2-148




341.80
306
310 (dec.)
A


 


2-149




306.33
307
>300
A


 


2-150




342.79
307
290 (dec.)
A


 


2-151




348.37
349
320 (dec.)
A


 


2-152




384.83
349
312 (dec.)
A


 


2-153




320.36
320
196-197
B


 


2-154




356.82
320
300 (dec.)
B


 


2-155




362.22
326
324 (dec.)
B


 


2-156




376.25
340
287 (dec.)
B


 


2-157




320.36
321
146-148
B


 


2-158




356.82
321
289 (dec.)
B


 


2-159




320.36
320
246-247
B


 


2-160




356.82
320
311 (dec.)
B


 


2-161




370.84
334
298 (dec.)
B


 


2-162




419.37
306
191 (dec.)
B


 


2-163




419.37
306
232 (dec.)
B


 


2-164




461.40
348
247 (dec.)
A


 


2-165




328.76
292
291 (dec.)
B


 


2-166




444.38
331
221 (dec.)
A


 


2-167




380.84
345
333 (dec.)
B


 


2-168




329.36
330
160 (dec.)
B


 


2-169




365.83
330
295 (dec.)
B


 


2-170




344.38
345
277-279
B


 


2-171




380.84
345
328 (dec.)
B


 


2-172




331.34
332
>300
A


 


2-173




367.80
332
287 (dec.)
A


 


2-174




356.39
356
296 (dec.)
B


 


2-175




392.82
356
270 (dec.)
B


 


2-176




446.82
410
248-249
B


 


2-177




342.36
342
275 (dec.)
B


 


2-178




296.35
297
187-188
B


 


2-179




332.81
297
310 (dec.)
A


 


2-180




330.80
330
198-199
B


 


2-181




367.26
330
298 (dec.)
B


 


2-182




346.84
310
>250
B


 


2-183




296.35
297
167 (dec.)
B


 


2-184




332.81
297
297 (dec.)
B


 


2-185




280.29
280
217-218
B


 


2-186




331.76
295
285 (dec.)
B


 


2-187




345.79
309
280-281
B


 


2-188




333.80
298
306 (dec.)
B


 


2-189




325.39
326
243 (dec.)
B


 


2-190




361.86
326
289-290
A


 


2-191




322.37
322
207-208
B


 


2-192




358.83
322
271-272
B


 


2-193




280.29
281
265 (dec.)
B


 


2-194




316.75
281
309-310
B


 


2-195




343.78
308
270-274 (dec.)
B


 


2-196




436.90
401
239
B


 


2-197




351.37
352
210-215 (dec.)
B


 


2-198




387.83
352
249 (dec.)
B


 


2-199




365.39
366
127
A


 


2-200




401.86
366
243 (dec.)
B


 


2-201




395.42
396
181
B


 


2-202




431.88
396
229 (dec.)
B


 


2-203




401.81
366
231 (dec.)
B


 


2-204




406.40
407
265-269 (dec.)
B


 


2-205




456.94
421
243-247 (dec.)
B


 


2-206




364.37
365
296
B


 


2-207




434.46
435
232-236 (dec.)
B


 


2-208




470.92
435
227
B


 


2-209




530.98
495
247
A


 


2-210




307.31
308
>300
B


 


2-211




343.78
308
>300
A


 


2-212




346.35
347
296 (dec.)
B


 


2-213




346.35
347
209
B


 


2-214




290.33
291
201-203 (dec.)
C


 


2-215




404.35
291
238-242
B


 


2-216




304.35
305
201-203
D


 


2-217




418.38
305
239-241
B


 


2-218




304.35
305
185-186
D


 


2-219




318.38
319
246-248
D


 


2-220




348.41
349
216-218
D


 


2-221




384.87
349
288 (dec.)
D


 


2-222




363.38
364
277 (dec.)
D


 


2-223




399.84
364
313 (dec.)
D


 


2-224




308.32
309
202-204
C


 


2-225




308.32
309
210-212
D


 


2-226




438.80
325
221-224
D


 


2-227




324.77
325
196-197
D


 


2-228




438.80
325
233-235
C


 


2-229




324.77
325
226-228
D


 


2-230




438.80
325
243-245
D


 


2-231




359.22
358
268-269
D


 


2-232




320.35
321
185-187
D


 


2-233




320.35
321
202-204
D


 


2-234




434.38
321
209-211
C


 


2-235




320.35
321
300 (dec.)
D


 


2-236




362.44
363
>410
D


 


2-237




386.84
351
259 (dec.)
D


 


2-238




386.84
351
274 (dec.)
B


 


2-239




350.38
351
330 (dec.)
D


 


2-240




416.87
381
291 (dec.)
D


 


2-241




364.41
365
248 (dec.)
D


 


2-242




400.87
365
321 (dec.)
D


 


2-243




336.42
337
169-170
D


 


2-244




372.88
337
292 (dec.)
D


 


2-245




368.42
369
278 (dec.)
D


 


2-246




404.88
369
320 (dec.)
D


 


2-247




369.40
370
278 (dec.)
C


 


2-248




405.87
370
308 (dec.)
C


 


2-249




403.85
403
240 (dec.)
D


 


2-250




440.31
403
300 (dec.)
D


 


2-251




449.35
336
198-200
D


 


2-252




335.32
334
265-267
D


 


2-253




449.35
336
238-239
D


 


2-254




335.32
334
279-281
D


 


2-255




449.35
336
265 (dec.)
D


 


2-256




429.36
316
248-250
D


 


2-257




419.37
306
175 (dec.)
D


 


2-258




333.40
334
188-190
D


 


2-259




369.86
334
266 (dec.)
D


 


2-260




447.42
334
240 (dec.)
D


 


2-261




388.48
389
218-222
D


 


2-262




461.40
348
253 (dec.)
D


 


2-263




347.38
348
208-210
D


 


2-264




383.84
348
304 (dec.)
D


 


2-265




405.46
406
280 (dec.)
D


 


2-266




355.40
356
218-220
D


 


2-267




391.86
356
309 (dec.)
D


 


2-268




356.39
357
267 (dec.)
D


 


2-269




392.85
357
324 (dec.)
D


 


2-270




356.39
357
209-211
D


 


2-271




392.85
357
319 (dec.)
D


 


2-272




348.36
349
224-226
D


 


2-273




348.36
349
253-255
D


 


2-274




434.46
435
289 (dec.)
D


 


2-275




470.92
435
282
D


 


2-276




291.31
292
204-205
C


 


2-277




405.34
292
206 (dec.)
C


 


2-278




291.31
292
224-225
C


 


2-279




405.34
292
2310 (dec.)
C


 


2-280




359.31
360
219-220
D


 


2-281




395.77
360
>250
C


 


2-282




334.38
335
249 (dec.)
D


 


2-283




370.84
335
311 (dec.)
C


 


2-284




343.78
308
346 (dec.)
D


 


2-285




321.34
322
198-199
C


 


2-286




351.37
352
244-245
D


 


2-287




387.83
352
210 (dec.)
C


 


2-288




337.41
338
233-234
D


 


2-289




373.87
338
298-299
C


 


2-290




339.79
340
213-214
B


 


2-291




325.76
326
246-247
B


 


2-292




292.30
293
267-268
C


 


2-293




406.33
293
234 (dec.)
C


 


2-294




306.33
307
257 (dec.)
C


 


2-295




420.35
307
231 (dec.)
C


 


2-296




293.33
294
128-129
C


 


2-297




329.79
294
264 (dec.)
C


 


2-298




280.29
281
350 (dec.)
C


 


2-299




316.75
281
311 (dec.)
C


 


2-300




394.31
281
230-232
B


 


2-301




330.80
331
198 (dec.)
D


 


2-302




310.38
311
192-193
C


 


2-303




341.35
342
286-287
D


 


2-304




377.81
342
300 (dec.)
D


 


2-305




341.35
342
269-270
D


 


2-306




377.81
342
296 (dec.)
D


 


2-307




298.33
299
219 (dec.)
C


 


2-308




380.84
345
344 (dec.)
B


 


2-309




440.43
441
250-253
D


 


2-310




445.36
332
252 (dec.)
B


 


2-311




373.42
374
202-203
D


 


2-312




347.40
348
303-305
D


 


2-313




383.86
348
314 (dec.)
C


 


2-314




343.39
344
259-260
D


 


2-315




343.39
344
288-289
D


 


2-316




341.38
342
263-264
D


 


2-317




377.84
342
319 (dec.)
B


 


2-318




377.84
342
316 (dec.)
D


 


2-319




374.43
375
260-261
D


 


2-320




410.89
375
310 (dec.)
D


 


2-321




374.43
375
281 (dec.)
D


 


2-322




410.89
375
335 (dec.)
D


 


2-323




334.38
335
167-168
D


 


2-324




310.38
311
122-123
D


 


2-325




320.35
321
149-150
D


 


2-326




228.26
229
189
D


 


2-327




242.28
243
amorphous
D


 


2-328




256.31
257
121-122
D


 


2-329




270.34
271
154 (dec.)
D


 


2-330




256.31
257
104-105
D


 


2-331




270.34
271
135-136
D


 


2-332




331.59
331
194 (dec.)
C


 


2-333




332.23
333
210-211
D


 


2-334




254.29
255
164-165
D


 


2-335




296.38
297
170-172
D


 


2-336




397.48
398
amorphous
D


 


2-337




431.50
432
119-120
D


 


2-338




397.48
398
147-148
D


 


2-339




297.36
298
179-180
D


 


2-340




397.48
398
amorphous
D


 


2-341




431.50
432
111-112
D


 


2-342




350.38
351
amorphous
C


 


2-343




288.31
289
240-241
D


 


2-344




302.34
303
224-225
D


 


2-345




334.38
335
269
C


 


2-346




339.42
340
272
D


 


2-347




376.42
377
244
D


 


2-348




381.46
382
124
D


 


2-349




364.35
365
226
B


 


2-350




400.81
365
292
C


 


2-351




375.25
376
232
D


 


2-352




411.71
376
275
C


 


2-353




325.76
326
254
B


 


2-354




330.80
331
228
C


 


2-355




330.80
331
174
C


 


2-356




367.26
331
276
B


 


2-357




325.76
326
243
C


 


2-358




330.80
331
233
D


 


2-359




367.26
331
227
C


 


2-360




309.31
310
242
C


 


2-361




314.34
214
315
C


 


2-362




450.34
336
224
C


 


2-363




341.80
306
204 (dec.)
D


 


2-364




383.88
348
230-240
D


 


2-365




370.80
335
274 (dec.)
D


 


2-366




341.80
306
270 (dec.)
D


 


2-367




428.88
398
273-274
A


 


2-368




403.83
368
240 (dec.)
A









Example 3-1
(Z)-2-Imidazo[1,2-c]quinazolin-5-yl-1-(2-thienyl)ethenol
(1) 2-(1H-Imidazol-2-yl)aniline




A mixture of 2-(4,5-dihydro-1H-imidazol-2-yl)aniline hydrobromide (50.0 mg, 0.207 mmol) and manganese dioxide (170 mg, 1.96 mmol) in N,N′-dimethylpropylenurea (2.0 mL) was heated at 150. (bath temp.). After 1 hour, the reaction mixture was cooled to room temperature, poured into a solution of hydroxylamine hydrochloride (0.5 g) in water (50 mL), and the resulting mixture was extracted with ethyl acetate. The separated organic layer was washed with brine, dried over magnesium sulfate, filtered, concentrated under reduced pressure. The crude residue was triturated with isopropylether, and the precipitate was removed by filtration. The filtrate was concentrated under reduced pressure, and the residue was purified by preparative thin layer chromatography (silica-gel, ethyl acetate as the eluent) to give 2-(1H-imidazol-2-yl)aniline (20 mg, 61% yield).
(2) Ethyl 3-oxo-3-(2-thienyl)propanoate




To a suspension of 2-thiophenecarboxylic acid (6.48 g, 50.57 mmol) in tetrahydrofurane (100 ml) at 5. was added 1,1′-Carbonyldiimidazole (8.61 g, 53.09 mmol) by portions. The mixture was allowed to warm to room temperature, and the stirring was continued for 1 hour. The reaction mixture was added into a suspension mixture of magnesium chloride (4.86 g, 51.07 mmol) and pottasium 3-ethoxy-3-oxopropanoate (12.91 g, 75.85 mmol) in tetrahydrofurane (50 ml). After being stirred at 50. for 2 hours and at room temperature overnight, the reaction mixture was poured into water, and then extracted with ethyl acetate. The extract was washed with brine, dried over magnesium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by column chromatography on silica-gel (ethyl acetate/hexane, 15/85) to give ethyl 3-oxo-3-(2-thienyl)propanoate (7.83 g, 78% yield) as a yellow oil.
(3) (Z)-2-Imidazo[1,2-c]quinazolin-5-yl-1-(2-thienyl)ethenol




A mixture of 2-(1H-imidazol-2-yl)aniline (60.0 mg, 0.38 mmol), ethyl3-oxo-3-(2-thienyl)propanoate (74.7 mg, 0.38 mmol) and p-tolenesulfonicacid monohydrate (36.1 mg, 0.19 mmol) in toluene (30 ml) was heated at reflux for 2 hours. After cooling to room temperature, the reaction mixture was poured into aqueous saturate NaHCO3 solution, and the resulting mixture was extracted with ethyl acetate. The extract was washed with brine, dried over magnesium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by column chromatography on silica-gel (ethyl acetate/hexane, 2/3-1/1) to give (Z)-2-imidazo[1,2-c]quinazolin-5-yl-1-(2-thienyl)ethenol (37.0 mg, 33% yeild) as a yellow powder.
Melting point: 128° C. Mass spectrometry: 294 In vitro PI3K-β inhibitory activity: In vitro PI3K-γ inhibitory activity: D 1H-NMR (300 MHz, CDCl3): d 6.11 (1H, s), 7.16 (1H, dd, J=3.8, 4.9 Hz), 7.34-7.41 (2H, m), 7.53-7.60 (3H, m), 7.64 (1H, d, J=1.7 Hz), 7.73 (1H, dd, J=1.1, 2.8 Hz), 8.34 (1H, dd, J=0.9, 7.8 Hz), 14.70 (1H, bs).
Example 3-2
(Z-2-Imidazo[1,2-c]quinazolin-5-yl-1-(2-thienyl)ethenol hydrochloride




To a solution of (Z-2-imidazo[1,2-c]quinazolin-5-yl-1-(2-thienyl)ethenol (0.06 g, 0.07 mmol) in chloroform (1.0 ml) was added a 4N solution of HCl in 1,4-dioxane (0.5 ml). The mixture was diluted with ethyl ether, and the resulting precipitate was collected by filtration, washed with ethyl ether, and dried under reduced pressure to give (Z)-2-imidazo[1,2-c]quinazolin-5-yl-1-(2-thienyl)ethenol hydrochloride (0.07 g, quantative) as a yellow solid.
Melting point: 263° C. (decomposition) Mass spectrometry: 294 In vitro PI3K-β inhibitory activity: In vitro PI3K-γ inhibitory activity: D 1H-NMR (300 MHz, DMSO-d6): δ 6.79 (1H, s), 7.28 (1H, dd, J=3.8, 4.9 Hz), 7.45 (1H, t, J=7.0 Hz), 7.66-7.77 (2H, m), 7.82 (1H, d, 1.7), 7.91 (1H, dd, J=1.1, 5.0 Hz), 8.17 (1H, dd, J=1.1, 3.8 Hz), 8.30 (1H, dd, J=1.0, 8.0 Hz), 8.62 (1H, d, J=1.7 Hz), 14.36 (1H, br).
Example 4-1
N-Imidazo[1,2-c]quinazolin-5-ylnicotinamide
(1) Imidazo[1,2]quinazolin-5-amine




To a solution of 2-(1H-imidazol-2-yl)aniline (0.06 g. 0.38 mmol) in methanol (3 ml) was added cyanogen bromide (0.05 g, 0.45 mmol). The resulting mixture was stirred at room temperature overnight. The reaction mixture was poured into water, and the resulting precipitate was collected by filtration, washed with acetone, and dried under reduced pressure to give imidazo[1,2-c]quinazolin-5-amine hydrobromide (0.06 g, 61% yield) as a white solid.
(2) N-Imidazo[1,2-c]quinazolin-5-ylnicotinamide




To a mixture of imidazo[1,2-c]quinazolin-5-amine hydrobromide (93 mg, 0.35 mmol) and nicotinic acid (124 mg, 1.01 mmol) and DMF (2.5 ml) at room temperature was added benzotriazole-1-yl-oxy-tris-pyrrolidino-phosphonium hexafluorophosphate (525 mg, 1.01 mmol) followed by N,N-diisopropylethyl amine (0.264 ml, 1.51 mmol), and the mixture was stirred at 80. for 6 hours. After cooling to room temperature, the reaction mixture was poured into aqueous saturated NaHCO3 solution. The resulting precipitate was collected by filtration, washed with acetone, and dried under reduced pressure to give N-imidazo[1,2-c]quinazolin-5-ylnicotinamide (40 mg, 39% yield) as a white solid.
Melting point: 223-224° C. (decomposition) Mass spectrometry: 290 In vitro PI3K-β inhibitory activity: In vitro PI3K-γ inhibitory activity: C 1H-NMR (300 MHz, DMSO-d6): d 7.53-7.62 (3 H, m), 7.70 (1H, t, J=7.34 Hz), 8.00 (1H, d, J=8.10 Hz), 8.30 (1H, d, J=7.91 Hz), 8.44 (1H, s), 8.63 (1H, d, J=7.72 Hz), 8.81 (1H, dd, J=1.5, 4.7 Hz), 9.49 (1H, s), 13.49 (1H, br).
Example 4-2
N-Imidazo[1,2-c]quinazolin-5-ylnicotinamide hydrochloride




To a solution of N-imidazo[1,2-c]quinazolin-5-ylnicotinamide (40 mg, 0.14 mmol) in methanol (20 ml) was added a 4N solution of HCl in 1,4dioxane (0.5 ml). The mixture was concentrated under reduced pressure. The resulting solid was collected by filtration, washed with tetrahydrofurane and dried under reduced pressure to give N-imidazo[1,2-c]quinazolin-5-ylnicotinamide hydrochloride (40 mg, 89% yield) as a white solid.
Melting point: 228° C. (decomposition) Mass spectrometry: 290 In vitro PI3K-β inhibitory activity: In vitro PI3K-γ inhibitory activity: C 1H-NMR (300 MHz, DMSO-d6): δ 7.60 (2H, br), 7.65 (1H, t, J=7.5 Hz), 7.82 (1H, dd, J=7.3, 8.1 Hz), 7.92 (1H, s), 8.02 (1H, dd, J=5.5, 7.9 Hz), 8.54 (1H, d, J=8.3 Hz), 8.73 (1H, s), 9.02 (1H, dd, J=1.3, 5.3 Hz), 9.07 (1H, d, J=7.53 Hz), 9.67 (1H, s).
REFERENCES


    [1] Wymann M P, Sozzani S, Altruda F. Mantovani A, Hirsch E: Lipids on the move: phosphoinositide 3-kinases in leukocyte function. Immunol. Today 2000; 6: 260-264.
    [2] Stein R C, Waterfield M D: PI3-kinase inhibition: a target for drug development? Mol. Med. Today. 2000; 6: 347-357.
    [3] Sean A. Weaver, Stephen G. Ward: Phosphoinositide 3-kinases in the gut: a link between inflammation and cancer? Trends in Molecular Medicine 2001;7:455-462.
    [4] Vanhaesebroeck B, Leevers S J, Panayotou G., Waterfield M D: Phosphoinositide 3-kinases: a conserved family of signal transducers. Trends Biochem. Sci. 1997; 22: 267-272.
    [5] Fruman D A, Meyers R E, Cantley L C: Phosphoinositide kinases. Annu. Rev. Biochem. 1998; 67: 481-507.
    [6] Wymann M P, Pirola L: Structure and function of phosphoinositide 3-kinases. Biochim. Biophys. Acta 1998; 1436: 127-150.
    [7] Sotsios Y, Ward S G: Phosphoinositide 3-kinase: a key biochemical signal for cell migration in response to chemokines. Immunol. Rev. 2000; 177: 217-235.
    [8] Toker A, Cantley L C: Signalling through the lipid products of phosphoinositide-3-OH kinase. Nature 1997; 387: 673-676.
    [9] Stephens L R, Jackson T R, Hawkins P T: Agonist-stimulated synthesis of phosphatidylinositol(3,4,5)-trisphosphate: a new intracellular signalling system? Biochim. Biophys. Acta. 1993; 1179: 27-75.
    [10] Stephens L R, Eguinoa A, Erdjumentbromage H, Lui M, Cooke F, Coadwell J, Smrcka A S, Thelen M, Cadwallader K, Tempst P, Hawkins P T: The G beta gamma sensitivity of a PI3K is dependent upon a tightly associated adaptor, p101. Cell 1997; 89:105-114.
    [11] Stoyanov B, Volinia S, Hanck T. Rubio I, Loubtchenkov M, Malek D, Stoyanova S, Van-Haesebroeck B, Dhand R, Nurnberg B, Gierschik P, Seedorf K, Hsuan J J, Waterfield M D, Wetzker R: Cloning and characterization of a G protein-activated human phosphoinositide-3 kinase. Science 1995; 269: 690-693.
    [12] Krugmann S, Hawkins P T, Pryer N, Braselmann S: Characterizing the interactions between the two subunits of the p101/p110gamma phosphoinositide 3-kinase and their role in the activation of this enzyme by G beta gamma subunits. J. Biol. Chem. 1999; 274: 17152-17158.
    [13] Sasaki T, Suzuki A, Sasaki J, Penninger J M: Phosphoinositide 3-kinases in immunity: lessons from knockout mice. J. Biochem. 2002; 131: 495-501.
    [14] Sasaki T, Irie-Sasaki J, Jones R G, Oliveira-dos-Santos A J, Stanford W L, Bolon B, Wakeham A, Itie A, Bouchard D, Kozieradzki I, Joza N, Mak T W, Ohashi P S, Suzuki A, Penninger J M: Function of PI3Kγ in thymocyte development, T cell activation, and neutrophil migration. Science 2000; 287: 1040-1046.
    [15] Li Z, Jiang H, Xie W, Zhang Z, Smrcka A V, Wu D: Roles of PLC-beta2 and -beta3 and PI3Kγ in chemoattractant-mediated signal transduction. Science 2000; 287: 1046-1049.
    [16] Hirsch E, Katanaev V L, Garlanda C, Azzolino O, Pirola L, Silengo L, Sozzani S, Mantovani A, Altruda F, Wymann M P: Central role for G protein-coupled phosphoinositide 3-kinase γ in inflammation. Science 2000; 287: 1049-1053.
    [17] Michael A. Crackower, Gravin Y. Oudit, Ivona Kozieradzki, Renu Sarao et al: Regulation of myocardial contractility and cell size by distinct PI3K-PTEN signaling pathways. Cell. 2002; 110: 737-749.
    [18] Emilio Hirsch, Ornella Bosco et al: Resistance to thromboembolism in PI3Kγ-deficient mice.The FASEB Journal. 2001; 15: 2019-2021.
    [19] Ui M, Okada T, Hazeki K, Hazeki O: Wortmannin as a unique probe for an intracellular signalling protein, phosphoinositide 3-kinase. Trends Biochem. Sci. 1995; 20: 303-307.
    [20] Vlahos C J, Matter W F, Hui K Y, Brown R F: A specific inhibitor of phosphatidylinositol 3-kinase, 2-(4-morpholino)-8-phenyl-4H-1-benzopyran-4-one (LY294002). J. Biol. Chem. 1994; 269: 5241-5248.

## Claims

1. A fused azolepyrimidine derivative of the formula (I), its tautomeric or stereoisomeric form, or a salt thereof:




wherein
X represents CR5R6 or NH;
Y1 represents CR3 or N;
Chemical bond between Y2═Y3 represents a single bond or double bond, with the proviso that when the Y2═Y3 represents a double bond, Y2 and Y3 independently represent CR4 or N, and
when Y2═Y3 represents a single bond, Y2 and Y3 independently represent CR3R4 or NR4;

Z1, Z2, Z3 and Z4 independently represent CH, CR2 or N;
R1 represents aryl optionally having 1 to 3 substituents selected from R11,
C3-8 cycloalkyl optionally having 1 to 3 substituents selected from R11,
C1-6 alkyl optionally substituted by aryl, heteroaryl, C1-6 alkoxyaryl, aryloxy, heteroaryloxy or one or more halogen,
C1-6 alkoxy optionally substituted by carboxy, aryl, heteroaryl, C1-6 alkoxyaryl, aryloxy, heteroaryloxy or one or more halogen,
or
a 3 to 15 membered mono- or bi-cyclic heterocyclic ring that is saturated or unsaturated, optionally having 1 to 3 substituents selected from R11, and contains 1 to 3 heteroatoms selected from the group consisting of N, O and S,
wherein
R11 represents
halogen, nitro, hydroxy, cyano, carboxy, amino, N—(C1-6alkyl)amino, N-(hydroxyC1-6alkyl)amino, N,N-di(C1-6alkyl)amino, N—(C1-6acyl)amino, N-(formyl)-N—(C1-6alkyl)amino, N—(C1-6alkanesulfonyl) amino, N-(carboxyC1-6-alkyl)-N—(C1-6alkyl)amino, N—(C1-6alkoxycabonyl)amino, N—[N,N-di(C1-6alkyl)amino methylene]amino, N—[N,N-di(C1-6alkyl)amino (C1-6alkyl)methylene]amino, N—[N,N-di(C1-6alkyl)amino C2-6alkenyl]amino, aminocarbonyl, N—(C1-6alkyl)aminocarbonyl, N,N-di(C1-6alkyl)aminocarbonyl, C3-8cycloalkyl, C1-6 alkylthio, C1-6alkanesulfonyl, sulfamoyl, C1-6alkoxycarbonyl,
N-arylamino wherein said aryl moiety is optionally having 1 to 3 substituents selected from R101, N-(aryl C1-6alkyl)amino wherein said aryl moiety is optionally having 1 to 3 substituents selected from R101, aryl C1-6alkoxycarbonyl wherein said aryl moiety is optionally having 1 to 3 substituents selected from R101,
C1-6alkyl optionally substituted by mono-, di- or tri-halogen, amino, N—(C1-6alkyl)amino or N,N-di(C1-6alkyl)amino,
C1-6alkoxy optionally substituted by mono-, di- or tri-halogen, N—(C1-6alkyl)sulfonamide, or N-(aryl)sulfonamide,
or
a 5 to 7 membered saturated or unsaturated ring having 1 to 3 heteroatoms selected from the group consisting of O, S and N, and optionally having 1 to 3 substituents selected from R101 
wherein

R101 represents halogen, carboxy, amino, N—(C1-6 alkyl)amino, N,N-di(C1-6alkyl)amino, aminocarbonyl, N—(C1-6alkyl)aminocarbonyl, N,N-di(C1-6alkyl)aminocarbonyl, pyridyl,
C1-6 alkyl optionally substituted by cyano or mono- di- or tri-halogen,
and
C1-6alkoxy optionally substituted by cyano, carboxy, amino, N—(C1-6alkyl)amino, N,N-di(C1-6alkyl)amino, aminocarbonyl, N—(C1-6alkyl)aminocarbonyl, N,N-di(C1-6alkyl)amino carbonyl or mono-, di- or tri-halogen;

R2 represents hydroxy, halogen, nitro, cyano, amino, N—(C1-6alkyl)amino, N,N-di(C1-6alkyl)amino, N-(hydroxyC1-6alkyl)amino, N-(hydroxyC1-6alkyl)-N—(C1-6alkyl)amino, C1-6 acyloxy, aminoC1-6 acyloxy, C2-6alkenyl, aryl,
a 5-7 membered saturated or unsaturated heterocyclic ring having 1 to 3 heteroatoms selected from the group consisting O, S and N, and optionally substituted by
hydroxy, C1-6 alkyl, C1-6 alkoxy, oxo, amino, amino C1-6alkyl, N—(C1-6alkyl)amino, N,N-di(C1-6alkyl)amino, N—(C1-6 acyl)amino, N—(C1-6alkyl)carbonylamino, phenyl, phenyl C1-6 alkyl, carboxy, C1-6alkoxycarbonyl, aminocarbonyl, N-(C1-6alkyl)aminocarbonyl, or N,N-di(C1-6alkyl)amino, —C(O)—R20 
wherein
R20 represents C1-6 alkyl, C1-6 alkoxy, amino, N—(C1-6alkyl)amino, N,N-di(C1-6alkyl)amino, N—(C1-6 acyl)amino, or a 5-7 membered saturated or unsaturated heterocyclic ring having 1 to 3 heteroatoms selected from the group consisting O, S and N, and optionally substituted by C1-6 alkyl, C1-6alkoxy, oxo, amino, N—(C1-6alkyl)amino, N,N-di(C1-6alkyl)amino, N—(C1-6 acyl)amino, phenyl, or benzyl,
C1-6 alkyl optionally substituted by R21,
or
C1-6 alkoxy optionally substituted by R21,
wherein
R21 represents cyano, mono-, di or tri-halogen, hydroxy, amino, N—(C1-6alkyl)amino, N,N-di(C1-6alkyl)amino, N-(hydroxyC1-6 alkyl) amino, N-(halophenylC1-6 alkyl) amino, amino C2-6 alkylenyl, C1-6 alkoxy, hydroxyC1-6 alkoxy, —C(O)—R201, —NHC(O)—R201, C3-8cycloalkyl, isoindolino, phthalimidyl, 2-oxo-1,3-oxazolidinyl, aryl or a 5 or 6 membered saturated or unsaturated heterocyclic ring having 1 to 4 heteroatoms selected from the group consisting O, S and N, and optionally substituted by hydroxy, C1-6 alkyl, C1-6 alkoxy, C1-6 alkoxycarbonyl, hydroxyC1-6 alkoxy, oxo, amino, aminoC1-6alkyl, N—(C1-6alkyl)amino, N,N-di(C1-6alkyl)amino, N—(C1-6 acyl)amino, or benzyl,
wherein
R201 represents hydroxy, amino, N—(C1-6alkyl)amino, N,N-di(C1-6alkyl)amino, N-(halophenylC1-6alkyl) amino, C1-6alkyl, aminoC1-6alkyl, aminoC2-6 alkylenyl, C1-6 alkoxy, a 5 or 6 membered saturated or unsaturated heterocyclic ring having 1 to 4 heteroatoms selected from the group consisting O, S and N, and optionally substituted by hydroxy, C1-6 alkyl, C1-6 alkoxy, C1-6 alkoxycarbonyl, hydroxyC1-6 alkoxy, oxo, amino, N—(C1-6alkyl)amino, N,N-di(C1-6alkyl)amino, N—(C1-6 acyl)amino or benzyl;


R3 represents hydrogen, halogen, aminocarbonyl, or C1-6 alkyl optionally substituted by aryl C1-6 alkoxy or mono-, di- or tri-halogen;
R4 represents hydrogen or C1-6 alkyl;
R5 represents hydrogen or C1-6 alkyl; and
R6 represents halogen, hydrogen or C1-6 alkyl.

2. The fused azolepyrimidine derivative of the formula (I), its tautomeric or stereoisomeric form, or a salt thereof as claimed in claim 1,
wherein
X represents CR5R6 or NH;
Y1 represents N;
Y2 and Y3 represent CR3R4;
Chemical bond between Y2═Y3 represents a single bond;
Z4 represents CH;
Z1, Z2 and Z3 independently represent CH, CR2 or N;
R1 represents
C1-6 alkyl optionally substituted by mono-, di- or tri-halogen, phenyl, methoxyphenyl, phenoxy, or thienyl,
C1-6 alkoxy optionally substituted by phenyl phenoxy, thienyl or mono-, di- or tri-halogen,
or
one of the following carbocyclic and heterocyclic rings selected from the group consisting of cyclopropyl, cyclopentyl, cyclohexyl, piperidinyl, piperazinyl, pyrrolyl, pyrazolyl, furyl, thienyl, thiazolyl, isothiazolyl, oxazolyl, isoxazolyl, imidazolyl, isoimidazolyl, pyrazolyl, 1,2,3-thiadiazolyl, 1,2,4-thiadiazolyl, 1,2,5-thiadiazolyl, 1,3,4-thiadiazolyl, 1,2,3-oxadiazolyl, 1,2,4-oxadiazolyl, 1,2,5-oxadiazolyl, 1,3,4-oxadiazolyl, 1,2,3-triazolyl, 1,2,4-triazolyl, 1,2,5-triazolyl, 1,3,4-triazolyl, phenyl, pyridyl, pyrazinyl, pyrimidinyl, pyridazinyl, 1-benzothiophenyl, benzothiazolyl, benzimidazolyl, 3H-imidazo [4,5-b]pyridinyl, benzotriazolyl, indolyl, indazolyl, imidazo[1,2-a]pyridinyl, quino linyl, and 1,8-naphthyridinyl,
wherein
said carbocyclic and heterocyclic rings optionally substituted with 1 to 3 substituents selected from the group consisting of hydroxy, halogen, nitro, cyano, carboxy, amino, N—(C1-6alkyl)amino, N-(hydroxyC1-6alkyl)amino, N,N-di (C1-6alkyl)amino, N—(C1-6acyl)amino, N—(C1-6alkoxycarbonyl)amino, N-(formyl)-N—(C1-6alkyl)amino, N,N-di(C1-6alkyl) amino (C2-6alkenyl) amino, N—(C1-6alkane)sulfonyl amino, N[N,N-di(C1-6alkyl)amino methylene]amino, C1-6 alkylthio, C1-6alkanesulfonyl, sulfamoyl, C1-6alkoxy, C1-6alkoxycarbonyl, pyrrolyl, imidazolyl, pyrazolyl, pyrrolidinyl, pyridyl, phenyl C1-6alkoxycarbonyl, thiazolyl optionally substituted by pyridyl, piperazinyl optionally substituted by C1-6 alkyl or C1-6alkoxy and C1-6alkyl optionally substituted by mono-, di- or tri-halogen;

R2 represents halogen, hydroxy, nitro, cyano, amino, N—(C1-6alkyl)amino, N,N-di(C1-6alkyl)amino, N-(hydroxyC1-6alkyl)-N—(C1-6alkyl)amino, (C2-6)alkenyl, C1-6alkoxycarbonyl, aminocarbonyl, furyl, piperidino, morpholino, phenyl, pyrrolidinyl optionally substituted by N—(C1-6 acyl)amino, or N—(C1-6 alkyl)carbonylamino, piperidino optionally substituted by hydroxy, piperazinyl optionally substituted by C1-6alkyl, phenylC1-6alkyl, C1-6alkoxycarbonyl, or aminocarbonyl;
C1-6 alkyl optionally substituted by amino, cyano, C1-6alkoxycarbonyl, morpholino, or mono-, di- or tri-halogen,
or
C1-6 alkoxy optionally substituted by hydroxy, cyano, carboxy, C1-6 alkoxy, C1-6 acyl, C1-6alkoxycarbonyl, amino, N—(C1-6alkyl)amino, N—(C1-6alkyl)aminocarbonyl, N,N-di(C1-6alkyl)amino, N,N-di(C1-6alkyl)aminocarbonyl, aminocarbonyl, aminoC1-6 alkylcarbonyl, N-(halobenzyl)aminocarbonyl, hydroxyC1-6 alkoxy, C3-6 cycloalkyl, morpholino, morpholinocarbonyl, pyrrolidinyl, pyrrolyl, piperidino, phthalimidyl,
or
piperazinyl optionally substituted by benzyl;


R3 represents hydrogen;
R4 represents hydrogen;
R5 represents hydrogen; and
R6 represents hydrogen.

3. The fused azolepyrimidine derivative of the formula (I), its tautomeric or stereoisomeric form, or a salt thereof as claimed in claim 1,
wherein
X represents CR5R6 or NH;
Y1 represents N;
Y2 and Y3 represent CR3R4;
Chemical bond between Y2═Y3 represents a single bond
Z4 represents CH;
Z1, Z2 and Z3 independently represent N, CH CR2;
R1 represents cyclopropyl, cyclopentyl, cyclohexyl, 2-furyl, 3-furyl, imidazolyl, pyrimidinyl, pyridazinyl, piperazinyl, 1,2,3-thiadiazolyl, 1,3-benzothiazolyl, quinolyl, 3H-imidazo[4,5-b]pyridinyl,
1H-pyrrol-2-yl optionally substituted by C1-6alkyl,
1H-pyrrol-3-yl optionally substituted by C1-6alkyl,
pyrazolyl optionally substituted by 1 or 2 C1-6alkyl,
isoxazolyl optionally substituted by 1 or 2 C1-6alkyl,
2-thienyl optionally substituted by chloro, nitro, cyano, or C1-6 alkyl,
3-thienyl optionally substituted by chloro, nitro, cyano, or C1-6 alkyl, piperidinyl optionally substituted by C1-6alkoxycarbonyl, or benzyloxycarbonyl, phenyl optionally substituted by 1 to 3 substituents selected from the group consisting of fluoro, chloro, hydroxy, nitro, cyano, carboxy, C1-6 alkyl, C1-6alkoxy, C1-6alkoxycarbonyl, amino, N—(C1-6alkyl)amino, N—(C1-6acyl)amino, N—(C1-6alkoxycabonyl)amino, N,N-di(C1-6alkyl)amino, N-(formyl)-N—C1-6alkylamino, C1-6 alkylthio, C1-6alkanesulfonyl, sulfamoyl, pyrrolyl, imidazolyl, pyrazolyl, and piperazinyl optionally substituted by C1-6alkyl,
pyridyl optionally substituted by 1 or 2 substituents selected from the group consisting of chloro, hydroxy, carboxy, C1-6alkoxy, C1-6alkylthio, amino, N—(C1-6alkyl)amino, N-(hydroxyC1-6alkyl)amino, N,N-di(C1-6alkyl)amino, N—(C1-6acyl)amino, N—(C1-6alkane)sulfonyl amino, N[N,N-di(C1-6alkyl)amino methylene]amino, and C1-6alkyl optionally substituted by tri halogen,
pyrazinyl optionally substituted by C1-6alkyl, 1,3-thiazolyl optionally substituted by 1 or 2 substituents selected from the group consisting of C1-6alkyl, pyridyl and N—(C1-6alkoxycrbonyl)amino, indolyl optionally substituted by C1-6alkyl,
benzimidazolyl optionally substituted by C1-6alkyl or tri-halo C1-6alkyl,
1,2,3-benzotriazolyl optionally substituted by C1-6alkyl,
1,8-naphthyridinyl optionally substituted by C1-6alkyl optionally substituted by tri halogen,
C1-6 alkyl optionally substituted by tri-halogen, phenyl, phenoxy, or thienyl,
or
C1-6alkoxy optionally substituted by phenyl, phenoxy, or thienyl;

R2 represents fluoro, chloro, bromo, hydroxy, nitro, vinyl, cyano, amino, aminoacetoxy, N—(C1-6alkyl)amino, N,N-di(C1-6alkyl)amino, N-(hydroxyC1-6alkyl)-N—(C1-6alkyl)amino, 2-furyl, piperidino, morpholino, phenyl, pyrrolidinyl optionally substituted by acetamido, piperidino optionally substituted by hydroxy, piperazinyl optionally substituted by methyl, benzyl, C1-6 alkoxycarbonyl, or aminocarbonyl,
C1-6 alkyl optionally substituted by cyano, tri-fluoro, carboxy, methoxycarbonyl, aminocarbonyl, tert-butoxycarbonyl, tetrahydropyranyl, or morpholino,
C1-6 alkoxy optionally substituted by hydroxy, cyano, methoxy, methoxycarbonyl, tert-butoxycarbonyl, carboxy, aminoacetyl, dimethylamino, aminocarbonyl, methylaminocarbonyl, dimethylamino carbonyl, isopropylaminocarbonyl, fluorobenzylaminocarbonyl, cyclopropyl, pyrrolidinyl, piperidino, tetrahydropyranyl, morpholino, morpholinocarbonyl, 2-oxo-1,3-oxazolidin-4-yl, phthalimid-N-yl, or hydroxy C1-6 alkyleneoxy,

R3 represents hydrogen;
R4 represents hydrogen;
R5 represents hydrogen; and
R6 represents hydrogen.

4. The fused azolepyrimidine derivative of the formula (I), its tautomeric or stereoisomeric form, or a salt thereof as claimed in claim 1,
wherein
X represents CR5R6 or NH;
Y1 represents N;
Y2 and Y3 represent CR3R4;
Chemical bond between Y2═Y3 represents a single bond;
Z3 and Z4 represent CH;
Z1 and Z2 independently represent CH or CR2;
R1 represents cyclopropyl, cyclopentyl, cyclohexyl, 2-furyl, 3-furyl, imidazolyl, 1H-pyrrol-2-yl, 1H-pyrrol-3-yl, pyrimidinyl, pyridazinyl, piperazinyl, 1,2,3-thiadiazolyl, 1,3-benzothiazolyl, quinolyl, 3H-imidazo [4,5-b]pyridinyl,
pyrrolyl optionally substituted by C1-6alkyl, pyrazolyl optionally substituted by 1 or 2 C1-6alkyl, isoxazolyl optionally substituted by 1 or 2 C1-6alkyl, 2-thienyl optionally substituted by chioro, nitro, cyano, or C1-6 alkyl, 3-thienyl optionally substituted by chloro, nitro, cyano, or C1-6 alkyl,
piperidinyl optionally substituted by C1-6alkoxycarbonyl, or benzyloxycarbonyl,
phenyl optionally substituted by 1 to 3 substituents selected from the group consisting of fluoro, chloro, hydroxy, nitro, cyano, carboxy, C1-6 alkyl, C1-6alkoxy, C1-6alkoxycarbonyl, amino, N—(C1-6alkyl)amino, N—(C1-6acyl)amino, N—(C1-6alkoxycabonyl)amino, N,N-di(C1-6alkyl)amino, N-(formyl)-N—C1-6alkylamino, C1-6 alkylthio, C1-6alkanesulfonyl, sulfamoyl, pyrrolyl, imidazolyl, pyrazolyl, and piperazinyl optionally substituted by C1-6alkyl,
pyridyl optionally substituted by 1 or 2 substituents selected from the group consisting of chloro, hydroxy, carboxy, C1-6alkoxy, C1-6alkylthio, amino, N—(C1-6alkyl)amino, N-(hydroxyC1-6alkyl)amino, N,N-di(C1-6alkyl)amino, N—(C1-6acyl)amino, N—(C1-6alkane)sulfonyl amino, N[N,N-di(C1-6alkyl)amino methylene]amino, and C1-6alkyl optionally substituted by tri halogen,
pyrazinyl optionally substituted by C1-6alkyl, 1,3-thiazolyl optionally substituted by
1 or 2 substituents selected from the group consisting of C1-6alkyl, pyridyl and N—(C1-6alkoxycrbonyl)amino, indolyl optionally substituted by C1-6alkyl, benzimidazolyl optionally substituted by C1-6alkyl or tri-halo C1-6alkyl,
1,2,3-benzotriazolyl optionally substituted by C1-6alkyl, 1,8-naphthyridinyl optionally substituted by C1-6alkyl optionally substituted by tri halogen,
C1-6 alkyl optionally substituted by tri-halogen, phenyl, phenoxy, or thienyl,
or
C1-6alkoxy substituted by phenyl, phenoxy, or thienyl;

R2 represents fluoro, chioro, bromo, hydroxy, nitro, vinyl, cyano, amino, aminoacetoxy, N—(C1-6alkyl)amino, N,N-di(C1-6alkyl)amino, N-(hydroxyC1-6alkyl)-N—(C1-6alkyl)amino, 2-furyl, piperidino, morpho lino, phenyl,
pyrrolidinyl optionally substituted by acetamido,
piperidino optionally substituted by hydroxy, piperazinyl optionally substituted by methyl, benzyl, C1-6alkoxycarbonyl, or aminocarbonyl,
C1-6 alkyl optionally substituted by cyano tri-fluoro, carboxy, methoxycarbonyl, aminocarbonyl, tert-butoxycarbonyl, tetrahydropyranyl, or morpholino,
or
C1-6 alkoxy optionally substituted by hydroxy, cyano, methoxy, methoxycarbonyl, tert-butoxycarbonyl, carboxy, aminoacetyl, dimethylamino, aminocarbonyl, methylaminocarbonyl, dimethylamino carbonyl, isopropylamino carbonyl, fluorobenzylaminocarbonyl, cyclopropyl, pyrrolidinyl, piperidino, tetrahydropyranyl, morpholino, morpholinocarbonyl, 2-oxo- 1,3-oxazolidin-4-yl, phthalimid-N-yl, or hydroxy C1-6 alkyleneoxy;

R3 represents hydrogen;
R4 represents hydrogen;
R5 represents hydrogen; and
R6 represents hydrogen.

5. The fused azolepyrimidine derivative of the formula (I), its tautomeric or stereoisomeric form, or a salt thereof as claimed in claim 1,
wherein
X represents CR5R6 or NH;
Y1 represents N;
Y2 and Y3 represent CR3R4;
Chemical bond between Y2═Y3 represents a single bond;
Z1 and Z4 represent CH;
Z2 and Z3 independently represent CH or CR2;
R1 represents cyclopropyl, cyclopentyl, cyclohexyl, 2-furyl, 3-furyl, imidazolyl, 1H-pyrrol-2-yl, 1H-pyrrol-3-yl, pyrimidinyl, piperazinyl, pyridazinyl, 1,2,3-thiadiazolyl, 1,3-benzothiazolyl, quinolyl, 3H-imidazo [4,5-b]pyridinyl,
pyrrolyl optionally substituted by C1-6alkyl,
pyrazolyl optionally substituted by 1 or 2 C1-6alkyl,
isoxazolyl optionally substituted by 1 or 2 C1-6alkyl,
2-thienyl optionally substituted by chloro, nitro, cyano, or C1-6 alkyl,
3-thienyl optionally substituted by chloro, nitro, cyano, or C1-6 alkyl,
piperidinyl optionally substituted by C1-6alkoxycarbonyl, or benzyloxycarbonyl,
phenyl optionally substituted by 1 to 3 substituents selected from the group consisting of fluoro, chloro, hydroxy, nitro, cyano, carboxy, C1-6 alkyl, C1-6alkoxy, C1-6alkoxycarbonyl, amino, N—(C1-6alkyl)amino, N—(C1-6acyl)amino, N—(C1-6alkoxycabonyl)amino, N,N-di(C1-6alkyl)amino, N-(formyl)-N—C1-6alkyl-amino, C1-6 alkylthio, C1-6alkanesulfonyl, sulfamoyl, pyrrolyl, imidazolyl, pyrazolyl, and piperazinyl optionally substituted by C1-6alkyl,
pyridyl optionally substituted by 1 or 2 substituents selected from the group consisting of chloro, hydroxy, carboxy, C1-6alkoxy, C1-6alkylthio, amino, N—(C1-6alkyl)amino, N-(hydroxyC1-6alkyl)amino, N,N-di(C1-6alkyl)amino, N—(C1-6acyl)amino, N—(C1-6alkane)sulfonyl amino, N[N,N-di(C1-6alkyl)amino-methylene]amino, C1-6alkoxyphenylC1-6alkoxy, and C1-6alkyl optionally substituted by tri halogen,
pyrazinyl optionally substituted by C1-6alkyl,
1,3-thiazolyl optionally substituted by 1 or 2 substituents selected from the group consisting of C1-6alkyl, pyridyl and N—(C1-6alkoxycrbonyl)amino, indolyl optionally substituted by C1-6alkyl, benzimidazolyl optionally substituted by C1-6alkyl or tri-halo C1-6alkyl,
1,2,3-benzotriazolyl optionally substituted by C1-6alkyl, 1,8-naphthyridinyl optionally substituted by C1-6alkyl optionally substituted by tri halogen,
C1-6 alkyl optionally substituted by tri-halogen, phenyl, phenoxy, or thienyl,
or
C1-6alkoxy substituted by phenyl, phenoxy, or thienyl;

R2 represents fluoro, chloro, bromo, hydroxy, nitro, vinyl, cyano, amino, aminoacetoxy, N—(C1-6alkyl)amino, N,N-di(C1-6alkyl)amino, N-(hydroxyC1-6alkyl)-N—(C1-6alkyl)amino, 2-furyl, piperidino, morpho lino, phenyl,
pyrrolidinyl optionally substituted by acetamido, piperidino optionally substituted by hydroxy, piperazinyl optionally substituted by methyl, benzyl, C1-6alkoxycarbonyl, or aminocarbonyl,
C1-6 alkyl optionally substituted by cyano, tri-fluoro, carboxy, methoxycarbonyl, aminocarbonyl, tert-butoxycarbonyl, tetrahydropyranyl, or morpholino,
or
C1-6 alkoxy optionally substituted by hydroxy, cyano, methoxy, methoxycarbonyl, tert-butoxycarbonyl, carboxy, aminoacetyl, dimethylamino, aminocarbonyl, methylaminocarbonyl, dimethylamino carbonyl, isopropylamino carbonyl, fluorobenzylamino carbonyl, cyclopropyl, pyrrolidinyl, piperidino, tetrahydropyranyl, morpho lino, morpho linocarbonyl, tetrazolyl, 2-oxo-1,3-oxazolidin-4yl, phthalimid-N-yl, or hydroxy C1-6 alkyleneoxy;

R3 represents hydrogen;
R4 represents hydrogen;
R5 represents hydrogen; and
R6 represents hydrogen.

6. The fused azolepyrimidine derivative of the formula (I), its tautomeric or stereoisomeric form, or a salt thereof as claimed in claim 1,
X represents CR5R6 or NH;
Y1 represents N;
Y2 and Y3 represent CR3R4;
Chemical bond between Y2═Y3 represents a single bond;
Z3 and Z4 represent CH;
Z1 and Z2 independently represent CH or CR2;
R1 represents 3H-imidazo [4,5-b]pyridinyl, benzimidazolyl
pyridyl optionally substituted by hydroxy, amino, acetamido, methoxybenzyloxy or methylsulfonylamino,
or
1,3-thiazolyl optionally substituted by 1 or 2 methyl;

R2 represents fluoro, chloro, bromo, morpholino, piperazinyl, methylpiperazinyl, methyl, tri-fluoro methyl, or C1-6 alkoxy optionally substituted by hydroxy, cyano, carboxy, dimethylaminocarbonyl, tetrahydropyranyl, morpholino, morpholinocarbonyl, tetrazolyl, or phthalimid-N-yl;
R3 represents hydrogen;
R4 represents hydrogen;
R5 represents hydrogen; and
R6 represents hydrogen.

7. The fused azolepyrimidine derivative of the formula (I), its tautomeric or stereoisomeric form, or a salt thereof as claimed in claim 1,
X represents CR5R6 or NH;
Y1 represents N;
Y2 and Y3 represent CR3R4;
Chemical bond between Y2═Y3 represents a single bond;
Z1, Z3 and Z4 represent CH;
Z2 represents CR2;
R1 represents 3H-imidazo [4,5-b]pyridinyl, benzimidazolyl pyridyl optionally substituted by hydroxy, amino, acetamido, methoxybenzyloxy or methylsulfonylamino,
or
1,3-thiazolyl optionally substituted by 1 or 2 methyl,

R2 represents fluoro, chloro, bromo, morpholino, piperazinyl, methylpiperazinyl, methyl, tri-fluoro methyl, C1-6 alkoxy optionally substituted by hydroxy, cyano, carboxy, dimethylaminocarbonyl, tetrahydropyranyl, morpholino, morpholinocarbonyl, tetrazolyl, or phthalimid-N-yl;
R3 represents hydrogen;
R4 represents hydrogen;
R5 represents hydrogen; and
R6 represents hydrogen.

8. The fused azolepyrimidine derivative of the formula (I), its tautomeric or stereoisomeric form, or a salt thereof as claimed in claim 1, wherein said derivative is selected from the group consisting of the following compounds:
N-(7,8-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;
2-(7,8-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1-pyridin-3-ylethyleno;
N-(7,8-dimethoxy-2,3-dihydroimidazol[1,2-c]quinazolin-5-yl)-1H-benzimidazole-5-carboxamide;
6-(acetamido)-N-(7,8-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;
N-{5-[2-(7,8-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1-hydroxyvinyl]pyridin-2-yl}acetamide;
2-({5-[2-hydroxy-2-pyridin-3-ylvinyl]-7-methoxy-2,3-dihydroimidazo[1,2-]quinazolin-8-yl}oxy)-N,N-dimethylacetamide;
2-[7-methoxy-8-(tetrahydro-2H-pyran-2-ylmethoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-1-pyridin-3-ylethylenol;
2-[8-(2-hydroxyethoxy)-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-1-pyridin-3-ylethylenol1;
({5-[2-hydroxy-2-pyridin-3-ylvinyl]-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-8-yl}oxy)acetic acid;
4-({5-[2-hydroxy-2-pyridin-3-ylvinyl]-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-8-yl}oxy)butanoic acid;
({5-[2-hydroxy-2-pyridin-3-ylvinyl]-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-8-yl}oxy)acetonitrile;
2-[7-methoxy-8-(2H-tetrazol-5-ylmethoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-1-pyridin-3-ylethyleno1;
2-[7-methoxy-8-(4-morpholin-4-yl-4-oxobutoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-1-pyridin-3-ylethylenol;
5-[1-hydroxy-2-(8-morpholin-4-yl-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)vinyl]pyridin-3-ol;
N-(2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-5-hydroxynicotinamide;
6-(acetamido)-N-(7,9-dimethoxy-8-methyl-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;
N-(8,9-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-5-hydroxynicotinamide;
5-hydroxy-N-(7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;
N-(7,8-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-5-[(4-methoxybenzyl)oxy]nicotinamide;
N-(7,8-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-5-hydroxynicotinamide;
5-hydroxy-N-[8-(trifluoromethyl)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide;
N-{8-[3-(1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)propoxy]-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl}nicotinamide;
N-(7-bromo-8-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;
6-amino-N-(8-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;
1-(1H-benzimidazol-5-yl)-2-(8,9-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)ethylenol;
2-(8,9-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1-(2,4-dimethyl-1,3-thiazol1-5-yl)ethylenol;
N-(9-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-l H-benzimidazole-5-carboxamide;
N-(8-bromo-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;
N-(8-bromo-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1H-benzimidazole-5-carboxamide;
N-(8-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1H-benzimidazole-5-carboxamide;
N-(8-methyl-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1H-benzimidazole-5-carboxamide;
N-[8-(trifluoromethyl)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-1H-benzimidazole-5-carboxamide;
N-(7-fluoro-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1H-benzimidazole-5-carboxamide;
N-(7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;
N-(8-chloro-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1H-benzimidazole-5-carboxamide;
6-(acetamido)-N-(8-morpholin-4-yl-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;
1-(1H-benzimidazol-5-yl)-2-(8-morpholin-4-yl-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)ethylenol;
N-{5-[1-hydroxy-2-(8-morpholin-4-yl-2,3-dihydroimidazol[1,2-c]quinazolin-5-yl)vinyl]pyridin-2-yl}acetamide;
6-methyl-N-(8-morpholin-4-y-2,3-dihydroimidazol[1,2-c]quinazolin-5-yl)nicotinamide;
1-(1H-benzimidazol-5-yl)-2-[8-(4-methylpiperazin-1-yl)-2,3-dihydroimidazol[1,2-c]quinazolin-5-yl]ethylenol;
N-(2,3-dihydroimidazol[1,2-c]quinazolin-5-yl)-3H-imidazo[4,5-b]pyridine-6-carboxamide;
N-(7,8-dimethoxy-2,3-dihydroimidazol[1,2-c]quinazolin-5-yl)-3H-imidazo[4,5-b]pyridine-6-carboxamide;
N-[7-(trifluoromethyl)-2,3-dihydroimidazol[1,2-c]quinazolin-5-yl]-1H-benzimidazole-5-carboxamide;
N-(7,9-dimethoxy-2,3-dihydroimidazol[1,2-c]quinazolin-5-yl)-1H-benzimidazole-5-carboxamide;
N-{5-[2-(7,9-dimethoxy-8-methyl-2,3-dihydroimidazol[1,2-c]quinazolin-5-yl)-1-hydroxyvinyl]pyridin-2-yl}acetamide;
N-{5-[2-(7-bromo-9-methyl-2,3-dihydroimidazol[1,2-c]quinazolin-5-yl)-1-hydroxyvinyl]pyridin-2-yl}acetamide; and
2-(8,9-dimethoxy-2,3-dihydroimidazol[1,2-c]quinazolin-5-yl)-1-pyridin-3-ylethylenol.

9. A medicament comprising the fused azolepyrimidine derivative, its tautomeric or stereoisomeric form, or a physiologically acceptable salt thereof as claimed in claim 1 as an active ingredient.

10. The medicament as claimed in claim 9, further comprising one or more pharmaceutically acceptable excipients.

11. The medicament as claimed in claim 9, wherein the fused azolepyrimidine derivative, its tautomeric or stereoisomeric form, or a physiologically acceptable salt thereof is a PI3K inhibitor.

12. The medicament as claimed in claim 9, wherein the fused azolepyrimidine derivative, its tautomeric or stereoisomeric form, or a physiologically acceptable salt thereof is a PI3K-γ inhibitor.