# Patent

## Title
Liquid pest control formulation

## Abstract

The present invention relates to a liquid pest control system that includes a synthetic pyrethroid as a pest control active ingredient and an agent selected from the group consisting of purified diethylene glycol monoethyl ether, tocopherol nicotinate and tocopherol succinate, and combinations thereof, to reduce or eliminate paraesthesia of the synthetic pyrethroid. The system releases the synthetic pyrethroid efficiently and uniformly. The pest control system is less irritating to the animal's skin as compared to prior art systems, particularly to the small breeds of dogs. The system is useful for making liquid spot-on treatments, sprays and the like.

## Background
This patent application claims the benefit of U.S. provisional patent application Ser. No. 61/249,968, filed Oct. 8, 2009, the disclosure of which is incorporated herein by reference in its entirety.


FIELD OF THE INVENTION
The present invention relates to a liquid pest control system that includes a synthetic pyrethroid as a pest control active ingredient and an agent to reduce or eliminate paraesthesia of the synthetic pyrethroid. The system releases the synthetic pyrethroid efficiently and uniformly. The pest control system is less irritating to the animal's skin as compared to prior art systems, particularly to the small breeds of dogs. The system is useful for making liquid spot-on treatments, sprays and the like.
BACKGROUND OF THE INVENTION
Many pest control active ingredients cause irritation (paraesthesia) to warm-blooded animals (including humans). This irritation to the skin and/or eyes of warm-blooded animals hampers the use of these pest control active ingredients. This irritation factor occurs even when the pest control active ingredient is blended with polymers or in other formulations (such as granules, dusts, dips, liquids, emulsions, etc.) wherein the active ingredient is considerably diluted.
The synthetic pyrethroid class of insecticides is known to cause paraesthesia when coming into contact with the skin, with differing degrees of paraesthesia being caused by different synthetic pyrethroids. Generally, the higher the degree of paraesthesia, the more active the pyrethroid is against various insects; and those having a cyano group in their molecular structure produce a greater degree of paraesthesia. While paraesthesia is a transitory phenomenon, higher degrees of paraesthesia have caused severe trauma to animals' skins when applied thereto and have required days for the pain to end and weeks for the skin to repair. Thus, it has prevented some of the most beneficial efficacious pyrethroids from being used on animals due to the unacceptable dermatological effects produced on the animal.
Efforts to prevent or reduce the paraesthesia effect of pyrethroids have been attempted with some success. However, in marketing these technologies to the total spectrum of the dog population, it was discovered that there is a small percentage of the total dog population, specifically the smallest dog breeds, that is more susceptible to pyrethroid-induced paraesthesia than the general population and that this paraesthesia in this group of small dogs is unacceptable.
It would be desirable to have a synthetic pyrethroid-containing product that not only reduces but actually eliminates paraesthesia, including in the small dog breeds, and to have a liquid synthetic pyrethroid-containing product that would be suitable for treatment of small animals.
SUMMARY OF THE INVENTION
The present invention is directed to a method and a composition for the controlled delivery of a pest control active agent or a mixture of active agents while reducing or eliminating the irritation of the pest control active agent to warm blooded mammals, including to the smaller dog breeds such as Chihuahua, Shih Tzu, Jack Russell and other small terriers, Papillion, Bussels Griffon, Japanese Chin, Praxsky Krysarik, and the like that are typically particularly susceptible to pyrethroid-induced paraesthesia. More particularly, the pest control system of the invention comprises a synthetic pyrethroid as the pest control active agent and an agent that reduces or eliminates paraesthesia (identified herein and in the appended claims as a “paraesthesia-reducing agent”) selected from the group consisting of purified diethylene glycol monoethyl ether, tocopherol nicotinate and tocopherol succinate, and combinations thereof.
The formulation of the invention may optionally include other ingredients as necessary or desired, depending on the particular synthetic pyrethroid chosen and the form and intended use of the final product. Such optional ingredients can include, but are not limited to, additional non-synthetic pyrethroid pesticidal active agents; system carriers such as water, solvents, and the like; synergists; fragrances; coloring agents; preservatives; antioxidants; light stabilizers; and the like. Examples of the resulting liquid pest control system include, but are not limited to, a dip, a spray, or a spot-on.
The present invention is further directed to a method for reducing or eliminating the irritation to warm-blooded animals, and especially to the small breeds of dogs, of a liquid synthetic pyrethroid in a liquid pest control system, the method comprising associating a paraesthesia-reducing agent (selected from the group consisting of purified diethylene glycol monoethyl ether, tocopherol nicotinate and tocopherol succinate, and combinations thereof) together with the synthetic pyrethroid in a pest control formulation, the amount of the paraesthesia-reducing agent(s) present being an amount effective to reduce or eliminate the irritation of the synthetic pyrethroid to warm blooded animals. Such amount can be determined by one of ordinary skill in the art following the teachings herein without undue experimentation.
The system of the invention provides a non paraesthesia-producing stable formulation that may, in one embodiment, include a high percentage of the irritating synthetic pyrethroid. This invention further allows for a high concentration of synthetic pyrethroid in a stable liquid formulation at room temperature, while reducing or eliminating any irritation of the synthetic pyrethroid. The formulation of the invention is effective without loss of the biological activity of the synthetic pyrethroid. This invention is particularly useful for reducing or eliminating the irritation of synthetic pyrethroids to the small dog breeds that are susceptible to pyrethroid-induced paraesthesia.


DETAILED DESCRIPTION OF THE INVENTION
As used herein, “a” and “an” mean one or more, unless otherwise indicated.
“Paraesthesia” as used herein and in the appended claims is defined as primarily a condition that results in a feeling (burning, tingling, and/or pricking sensation) of the skin.
To “eliminate the parasthesia”, as used herein and in the appended claims, means that a formulation has a Paraesthesia Rating of less than 1 for up to at least 24 hours in the Human Ear Assay, as described in the Examples hereinbelow.
As used herein, a “paraesthesia-reducing agent” is an agent that reduces or eliminates paraesthesia, particularly in the small breeds of dogs, and is selected from the group consisting of purified diethylene glycol monoethyl ether, tocopherol nicotinate and tocopherol succinate, and combinations thereof.
The pest control active agent may be chosen from any active agent known to cause paraesthesia in warm-blooded animals, such as but not limited to a synthetic pyrethroid. While the invention is particularly useful for delivering liquid synthetic pyrethroids at high concentrations in the formulation, the invention is not limited thereto but may also be used with synthetic pyrethroids at any concentration that results in the desired result of reducing or eliminating the paraesthetic effects of the paraesthesia-causing active agent while maintaining the active agent's pesticidal effects. One or more pesticidally active agents may be included within the formulation of the present invention, which may include active agents other than synthetic pyrethroids and active agents that do not cause paraesthesia, as long as at least one active agent in the formulation is a synthetic pyrethroid. Exemplary pesticides and repellents which are effective against horn flies, face flies, stable flies, house flies, mosquitoes, lice, ticks, and mites are pyrethrin, cypermethrin, decamethrin, cyhalothrin, flumethrin, cyfluthrin, fenvalerate, deltamethrin, fempropathrin, fluvalinate, flucythrinate, cyfluthrin, alphamethrin, tralomethrin, cycloprothrin, karate, cyphenothrin (GokilahtTM), or any synthetic pyrethroid with a cyano group in its molecular structure. One one embodiment, the pest control active agent is a synthetic pyrethroid having a cyano group in its molecular structure.
Many of these active agents are effective both as a pesticide and a repellent, and the activity of many is enhanced by the inclusion of a synergist. Suitable synergists are known to those of skill in the art or can be determined without undue experimentation, examples of which include but are not limited to piperonyl butoxide and N-octyl bicycloheptene dicarboximide.
The second component of the composition of the present invention is a paraesthesia-reducing agent selected from the group consisting of purified diethylene glycol monoethyl ether (CTFA/INCI Name: ethoxydiglycol), tocopherol nicotinate and tocopherol succinate, and combinations thereof. In one embodiment, only one paraesthesia-reducing agent is present in the formulation. In one embodiment, a combination of two of the paraesthesia-reducing agents is present in the formulation. In one embodiment, all three of the paraesthesia-reducing agents are present in the formulation.
The diethylene glycol monoethyl ether must meet the following specifications to be specified as “purified” for use in this invention:
















Density
 0.978-0.9980



refractive Index at 20° C.
1.4260-1.4280



Water Content
0.00-0.10











Acid Value
0.00-0.10
mg KOH/g



Peroxide Value
0.00-8.00
mg KOH/g



Boiling Point
195-210°
C.



Oxide Ethylene Content
0.00-1.00
ppm



Ether Monomethylique EG Content
0-50
ppm



Ethylene Glycol Content
0-620
ppm



Diethylene Glycol Content
0.250
ppm










Apparented Substances Total
  0.00-0.20%










This ether is compatible with high concentrations (that is, of up to about 30 wt % or more, or of up to about 50 wt % or more, or of up to about 90 wt % or more) of liquid synthetic pyrethroids.

The amount of total paraesthesia-reducing agent(s) in the formulation relative to the amount of synthetic pyrethroid will be an amount effective to reduce or eliminate the irritation or paraesthesia caused by the synthetic pyrethroid. The effective amount is easily determinable by routine experimentation following the teachings herein. Generally, the total amount of paraesthesia-reducing agent(s) in the formulation should be at least equal to the amount of synthetic pyrethroid and, often, the amount of the paraesthesia-reducing agent(s) in the formulation is double to many times the amount of synthetic pyrethroid in the formulation in order to reduce the irritation value of the active agent. Thus, the ratio of paraesthesia-reducing agent(s) to synthetic pyrethroid in one embodiment can be from about 1:1 to about 99.9:0.1. In one embodiment, the ratio can be from about 1:1 to about 5:1. In one embodiment, the ratio can be from about 1:1 to about 2:1.
When both tocopherol nicotinate and tocopherol succinate are present in the formulation, the ratio of nicotinate to succinate is from about 1:1 to about 3:1.
To prepare pest control systems according to the invention, the synthetic pyrethroid pest control active agent and the ether component are mixed with other ingredients as necessary or desired, depending on the particular active agent chosen and the form and intended use of the final product. The resulting formulation is then processed into the desired pest control system.
For example, the one or more pest control agent(s) and one or more paraesthesia-reducing agents are mixed together with a suitable organic solvent, an aqueous solvent, or mixtures thereof. The liquid carrier is chosen such that the active agent(s) together with any optional additional ingredients form a liquid final product.
The following examples illustrate the practice of the present invention. Parts are given as percentages and temperature in degrees Fahrenheit unless otherwise noted. “RT” is room temperature.
EXAMPLES
Example 1
Manufacturing Procedure
Formulations A-LL (see, Table 1 below) were prepared as follows: All ingredients were weighed and added to a heatable vessel. The vessel was the heated to 140° F., and the ingredients were stirred until a homogenous mixture was achieved. The mixture was then cooled to room temperature.









TABLE 1









Ingredients (wt %)


















Tocopherol
Tocopherol


Formulation
Gokilaht*
Nylar**
solvent{circumflex over ( )}
Transcutol CG†
Nicotinate#
Succinate#
















A
42.5
2.1
—
55.4
—
—


B
32.5
2.1
—
65.4
—
—


C
22.5
2.1
—
75.4
—
—


D
12.5
2.1
—
85.4
—
—


E
2.5
2.1
—
95.4
—
—


F
1.0
2.1
—
96.9
—
—


G
42.5
2.1
—
12.9
42.4
—


H
32.5
2.1
—
33.0
32.4
—


I
22.5
2.1
—
53.0
22.4
—


J
12.5
2.1
—
73.0
12.4
—


K
2.5
2.1
—
90.4
5.0
—


L
1.0
2.1
—
95.0
1.0
—


M
42.5
2.1
—
25.4
—
30.0


N
22.5
2.1
—
60.4
—
15.0


O
2.5
2.1
—
92.9
—
2.5


P
1.0
2.1
—
96.19
—
0.71


Q
42.5
2.1
—
14.6
30.4
10.4


R
32.5
2.1
—
34.5
23.2
7.7


S
22.5
2.1
—
54.0
16.0
5.4


T
12.5
2.1
—
73.5
8.9
3.0


U
2.5
2.1
—
93.0
1.8
0.6


V
1.0
2.1
—
95.9
0.75
0.25


W
42.5
2.1
12.9
—
42.4
—


X
32.5
2.1
33.0
—
32.4
—


Y
22.5
2.1
53.0
—
22.4
—


Z
12.5
2.1
73.0
—
12.4
—


AA
2.5
2.1
90.4
—
5.0
—


BB
1.0
2.1
95.0
—
1.0
—


CC
42.5
2.1
25.4
—
—
30.0


DD
22.5
2.1
60.4
—
—
15.0


EE
2.5
2.1
92.9
—
—
2.5


FF
1.0
2.1
96.19
—
—
0.71


GG
42.5
2.1
14.6
—
30.4
10.4


HH
32.5
2.1
34.5
—
23.2
7.7


II
22.5
2.1
54.0
—
16.0
5.4


JJ
12.5
2.1
73.5
—
8.9
3.0


KK
2.5
2.1
93.0
—
1.8
0.6


LL
1.0
2.1
95.9
—
0.75
0.25





*Gokilaht TM Technical 94.5% (d-cyphenothrin; synthetic pyrethroid); available from MGK Company.


**Nylar ® Technical 100% (insect growth regulator; comprises approx. 50 wt % pyriproxyfen and approx. 50 wt % corn oil); available from MGK Company.


{circumflex over ( )}aliphatic solvent, such as Isopar M TM (available from Exxon Chemicals).


†Transcutol CG is purified diethylene glycol monoethyl ether; supplied by Gattefosse Corp.


#Tocopherol Nicotinate and Tocopherol Succinate available from Sigma-Aldrich Corporation.






Testing:

The human ear assay is capable of discriminating between the effects of various formulations. Rapid penetration of the active agent in the ear lobe as well as the presence of the inferior maxillary of the trigeminus nerve system of the ear lobe tend to increase the sensitivity of the assay. It has been determined that a total volume of 10 μL of a formulation with an active volume (%) adjusted to 4 μL of technical active and applied to a human ear lobe provides, over time, an acceptable method of rating the paraesthesia of formulations.
For purposes of evaluation of the discovery of this invention and to assist in determining the useful ratios of active agent to the paraesthesia-reducing agent the following screening procedure was set up:

    
    
        1. Formulations are selected for evaluation.
        2. A 10 μL sample of formulation to be evaluated is pipetted onto the ear lobe and left physically undisturbed. The paraesthetic effect of this sample is evaluated within a 24-hour period.
        3. The “Paraesthesia Rating” is taken at the following time intervals following application of the formulation: 15 min, 30 min, 45 min, 1 hour and each hour thereafter to 24 hours.
        4. Irritation or sensitivity values are assigned according to the following scale (“Paraesthesia Rating”):
    
    
















 0 = no sensation



 1 = slight sensation



 2 = sensation/no discomfort



 3 = noticeable sensation



 4 = slight discomfort



 5 = noticeable discomfort



 6 = uncomfortable



 7 = very uncomfortable



 8 = moderate hurting



 9 = hurting



10 = severe hurting









The formulations A-V in Table 1 were screened following this procedure and were found to have a paraesthesia rating (“PR”) of less than 1 at any period within 24 hours and 0 at 24 hours. Formulations W and GG were tested and were found to have a paraesthesia rating (“PR”) of less than 1 at any period within 24 hours and 0 at 24 hours.
Example 2
Formulation “G” was tested on three Shih Tzu puppies with family history of being susceptible to cyphenothrin-induced paraesthesia. The animals were examined and found to be in good health and happy. The animals were treated with 1.5 mL of formulation (1.45 gm/0.655 gm cyphenothrin (0.687 gm technical Gokilaht)). Treatment was made by applying the dose to the animal's back, from the back of the neck to the back shoulders. The puppies were visually observed for signs of irritation or any reactions to the application of the formulation. The three puppies showed no signs of paraesthesia reactions at 0 to 31 hours.
Example 3
Formulation “G” was tested on two adult (2-year-old) Chihuahuas following the procedure of Example 3. Neither dog showed any signs of paraesthesia reactions at 0 to 31 hours.

## Claims

1. A liquid pest control formulation consisting of cyphenothrin and a paraesthesia-reducing agent, wherein the paraesthesia-reducing agent is purified diethylene glycol monoethyl ether and is present in an amount of from about 50 wt % to about 99 wt % to reduce or eliminate paraesthesia of the cyphenothrin on dogs.

2. A liquid pest control formulation according to claim 1 wherein the cyphenothrin is present in an amount of from about 1 wt % to about 50 wt %.

3. A liquid pest control formulation consisting of cyphenothrin, an insect growth regulator, and a paraesthesia-reducing agent, wherein the paraesthesia-reducing agent is purified diethylene glycol monoethyl ether and is present in an amount of from about 50 wt % to about 99 wt % to reduce or eliminate paraesthesia of the cyphenothrin on dogs.

4. A liquid pest control formulation according to claim 3 wherein the insect growth regulator is pyriproxyfen.

5. A liquid pest control formulation consisting of a synthetic pyrethroid and a paraesthesia-reducing agent, wherein the paraesthesia-reducing agent is purified diethylene glycol monoethyl ether and is present in an amount of from about 50 wt % to about 99 wt % to reduce or eliminate paraesthesia of the synthetic pyrethroid on dogs.

6. A liquid pest control formulation consisting of cyphenothrin in an amount of from about 1 wt % to about 50 wt % and two paraesthesia-reducing agents selected from the group consisting of purified diethylene glycol monoethyl ether and tocopherol nicotinate, in an amount effective to reduce or eliminate paraesthesia of the cyphenothrin on dogs.

7. A method for reducing or eliminating paraesthesia caused by a synthetic pyrethroid to mammals, the method comprising treating a mammal with the liquid pest control formulation as defined in claim 1.

8. A method according to claim 7 wherein the mammal is a dog.

9. A method according to claim 7 wherein the mammal is a small breed of dog.