# Patent

## Title
Process for preparation of 2-methyl-2′ phenylpropionic acid derivatives and novel intermediate compounds

## Abstract

The present invention relates to a process for preparing 2-methyl-2′-phenylpropionic acid derivatives showing antihistamine activity in more simplified way, intermediate compounds and their preparation processes used therefor. According to the present invention, pharmaceutically useful 2-methyl-2′-phenylpropionic acid derivatives can be prepared with high yield and purity on industrial scale.

## Background
BACKGROUND OF THE INVENTION
(a) Field of the Invention
The present invention relates to a process for preparing 2-methyl-2′-phenylpropionic acid derivatives, novel intermediate compounds and their preparation processes used therefor.
(b) Description of the Related Art
2-methyl-2′-phenylpropionic acid derivatives of the following Formula 1 show excellent antihistamine activity and antiallergic activity, and thus widely used in the field of pharmaceutics.




wherein, A is oxygen or nitrogen; R1 is hydrogen or C1-C6 linear or branched alkyl when A is oxygen, and R1 together with A forms a 5 to 7-membered ring unsubstituted or substituted with C1-C6 linear or branched alkyl when A is nitrogen; R2 is hydrogen or —CH2CH2OR2′, provided that R2 is —CH2CH2OR2′ when A is nitrogen; and R2′ is hydrogen, C1-C6 linear or branched alkyl, C3-C6 cyclic alkyl, or C2-C6 alkenyl.
Particularly, 2-methyl-2′-phenylpropionic acid derivatives exclusively have H1 antihistamine activity. Thus, they show high selectivity without acting with other pharmaceutical receptors even at higher dose. Therefore, 2-methyl-2′-phenylpropionic acid derivatives can be useful for a patient having allergic diseases, particularly for a patient who simultaneously receives other medicines, for example, those having cardiovascular disease (U.S. Pat. No. 5,877,187).
Meanwhile, EP 0818454 discloses a process for preparing 2-methyl-2′-phenylpropinoic acid derivatives belong under the Formula 1. However, this process majorly has two disadvantages as follows.
First, preparation of an intermediate compound having oxaozle group introduced therein and hydrolysis of oxazole group are inevitably comprised in the process, making the whole process complicated. Second, in an another intermediate compound, N—H bonds capable of N-alkylation exist at imidazole group as well as piperidinyl group, and thus, plenty of by-products may be generated. Accordingly, the process is inefficient to obtain 2-methyl-2′-phenylpropionic acid derivatives of the Formula 1 on industrial scale.
Meanwhile, J. Med. Chemistry (1986, 29, 1178-1183) and J. Heterocyclic Chem. (1987, 24, 31-37) describes a process for preparing piperidinyl benzoimidazole derivatives. However, this process use highly inflammable zinc powder in the step of reducing nitro group to amine group, and the yield is very low as about 30%, thus inappropriate for industrial scale production.
For the above reasons, there still have been demands for improvement of yield or purity of pharmaceutically useful 2-methyl-2′-phenylpropionic acid derivatives and their preparation process suitable for industrial scale production.
SUMMARY OF THE INVENTION
Thus, the present invention is to provide a process for preparing 2-methyl-2′-phenylpropionic acid derivatives, which is suitable for industrial scale production of pharmaceutically useful 2-methyl-2′-phenylpropionic acid derivatives with higher yield.
Further, the present invention is to provide novel intermediate compounds that can be used in the preparation of 2-methyl-2′-phenylpropionic acid derivatives.
Moreover, the present invention is to provide a process for preparing the above intermediate compounds.
The present invention provides a process for preparing 2-methyl-2′-phenylpropionic acid derivatives of the following Formula 1, comprising the step of reacting a compound of the following Formula 2 with a compound of the following Formula 3:




wherein, A is oxygen or nitrogen; R1 is hydrogen or C1-C6 linear or branched alkyl when A is oxygen, and R1 together with A forms a 5 to 7-membered ring unsubstituted or substituted with C1-C6 linear or branched alkyl when A is nitrogen; R2 is hydrogen or —CH2CH2OR2′, provided that R2 is —CH2CH2OR2′ when A is nitrogen; R2′ is hydrogen, C1-C6 linear or branched alkyl, C3-C6 cyclic alkyl, or C2-C6 alkenyl; and X is a leaving group.
The present invention also provides a compound of the following Formula 1a, which may be used as an intermediate for preparing 2-methyl-2′-phenylpropionic acid derivatives:




wherein R1 is hydrogen or C1-C6 linear or branched alkyl.
Further, the present invention provides a compound of the following Formula 2a which may be used as an intermediate for preparing 2-methyl-2′-phenylpropionic acid derivatives, and the preparation thereof.




wherein R1 is hydrogen or C1-C6 linear or branched alkyl, and X is a leaving group.
The present invention also provides a compound of the following Formula 6 which may be used as an intermediate for preparing 2-methyl-2′-phenylpropionic acid derivatives, and the preparation thereof.




wherein R3 is C1-C6 linear or branched alkyl, B is hydrogen or silyl protecting group.
The present invention also provides a novel process for preparing a compound of the following Formula 3a which may be used as an intermediate for preparing 2-methyl-2′-phenylpropionic acid derivatives:




wherein R2′ is hydrogen, C1-C6 linear or branched alkyl, C3-C6 cyclic alkyl, or C2-C6 alkenyl.


DETAILED DESCRIPTION OF THE EMBODIMENTS
A process for preparing 2-methyl-2′-phenylpropionic acid derivatives, intermediate compounds and their preparation processes used therefore according to aspects of the present invention will now be explained in more detail.
In one aspect, a process for preparing 2-methyl-2′-phenylpropionic acid derivatives of the following Formula 1 is provided. The process comprises the step of reacting a compound of the following Formula 2 with a compound of the following Formula 3:




wherein, A is oxygen or nitrogen; R1 is hydrogen or C1-C6 linear or branched alkyl when A is oxygen, and R1 together with A forms a 5 to 7-membered ring unsubstituted or substituted with C1-C6 linear or branched alkyl when A is nitrogen; R2 is hydrogen or —CH2CH2OR2′, provided that R2 is —CH2CH2OR2′ when A is nitrogen; R2′ is hydrogen, C1-C6 linear or branched alkyl, C3-C6 cyclic alkyl, or C2-C6 alkenyl; and X is a leaving group.
In the above formulas, although not limited thereto, X may be a common leaving group suitable for reaction between the compound of the Formula 2 and the compound of the Formula 3, for example, halogen, sulfinyloxy group or sulfonyloxy group.
In this process, the compounds of the Formulas 2 and 3 can be reacted in the presence of a base in an organic solvent. As the base, for example, an inorganic base such as sodium C1-C6 alkoxide, potassium C1-C6 alkoxide, sodium carbonate, potassium carbonate, lithium carbonate, cesium carbonate, sodium bicarbonate, potassium bicarbonate, or potassium phosphate, or an organic base such as 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU), 1,4-diazabicyclo[2.2.2]octane (DABCO), 1,5-diazabicyclo[4.3.0]non-5-ene (DBN), pyridine, dimethylaminopyridine or triethylamine can be used. Preferably, an inorganic base such as sodium C1-C6 alkoxide, potassium C1-C6 alkoxide, sodium carbonate, potassium carbonate, lithium carbonate, cesium carbonate, sodium bicarbonate, potassium bicarbonate, or potassium phosphate can be used. Thereby, reaction between the compounds of the Formulas 2 and 3 are preferably progressed to form a compound of the Formula 1.
Meanwhile, when R1 of the Formula 1 is alkyl, after the reaction between the compounds of the Formulas 2 and 3, the reaction product can be ester-hydrolyzed to prepare 2-methyl-2′-phenylpropionic acid derivative of the Formula 1 in the form of an acid.
Further, when R2 of the reaction product obtained by the reaction between the compounds of the Formulas 2 and 3 is hydrogen (for example, the reaction product is a compound of the following Formula 1a), if necessary, the reaction product can be reacted with a compound of the following Formula 9 to prepare 2-methyl-2′-phenylpropionic acid derivative of the Formula 1.




wherein R1 is hydrogen or C1-C6 linear or branched alkyl, R2′ is hydrogen, C1-C6 linear or branched alkyl, C3-C6 cyclic alkyl, or C2-C6 alkenyl, and Y is a leaving group.
In the process according one aspect of the present invention, the compound of the Formula 2 may be represented by the following Formula 2a wherein R1 is hydrogen or C1-C6 linear or branched alkyl when A is oxygen, or represented by the following Formula 2b wherein R1 together with A forms a 5 to 7-membered ring unsubstituted or substituted by C1-C6 linear or branched alkyl when A is nitrogen.




wherein R1 is hydrogen or C1-C6 linear or branched alkyl, and X is a leaving group.
The compound of the Formula 2b can be easily prepared by a process publicly known in the art (see European Patent No. 0818454). Different from this compound, the compound of the Formula 2a is a novel intermediate compound useful for the preparation of 2-methyl-2′-phenylpropionic acid derivatives. Using this novel intermediate compound, 2-methyl-2′-phenylpropionic acid derivatives of the Formula 1 can be prepared in a more simplified process with high yield.
The compound of the Formula 2a can be prepared by substituting —OB group in a novel intermediate compound of the following Formula 6 with a leaving group X:




wherein R3 is C1-C6 linear or branched alkyl, and B is hydrogen or silyl protecting group.
Further, the compound of the following Formula 6 can be prepared by reacting a compound of the following Formula 4 with a compound of the following Formula 5.




wherein Hal is halogen, R3 is C1-C6 linear or branched alkyl, and B is hydrogen or silyl protecting group.
In the process, the compounds of the Formulas 4 and 5 are commercially available.
The compounds of the Formulas 4 and 5 can be reacted in the presence of Pd catalyst in an organic solvent. As the Pd catalyst, Pd(0) catalyst such as Pd(dba)2, Pd2(dba)3 or Pd(t-Bu3P)2 can be used, and Pd(dba)2 is preferable.
When B of the Formula 4 is hydrogen, if necessary, before the reaction of the compound of the Formula 4 and the compound of the Formula 5, a silyl protecting group can be introduced in the compound of the Formula 4. After that, the compound of the Formula 4 having the silyl protecting group introduced therein can be reacted with the compound of the Formula 5.
The silyl protecting group can be selected from the group consisting of hexamethyldisilyl, trimethylsilyl, tert-butyldimethylsilyl, triisopropylsilyl and tert-butyldiphenylsilyl. Preferably, hexamethyldisilyl, trimethylsilyl or tert-butyldimethylsilyl can be introduced.
And, if necessary, the compound of the Formula 6 which is prepared by the reaction of the compound of the Formula 4 having silyl protecting group introduced therein and the compound of the Formula 5 can be used in a subsequent reaction after deprotection of the silyl protecting group.
After the formation of the compound of the Formula 6, —OB group in the compound of the Formula 6 is substituted with the leaving group X to form the compound of the Formula 2a.
The leaving group X can be a common leaving group suitable for the reaction with the compound of the Formula 3, for example, halogen, sulfinyloxy group, or sulfonyloxy group. Thus, under common substitution reaction conditions according to the kinds of the leaving group, —OB group of the compound of the Formula 6 can be substituted with the leaving group X to obtain the compound of the Formula 2a. For example, in case the leaving group X is halogen, the compound of the Formula 6 can be reacted with common halogenation reagents to obtain the compound of the Formula 2a; and in case the leaving group X is sulfinyloxy or sulfonyloxy group, the compound of the Formula 6 can be reacted with common sulfinylation or sulfonylation reagents (for example, sulfinyl halide or sulfonyl halide) to obtain the compound of the Formula 2a.
In the preparation process of the compound of the Formula 2a, in the case where R1 in the compound of the Formula 2a and R3 in the compound of the Formula 6 are different to each other, if necessary, R3 of the Formula 6 can be substituted with R1, and then substitution with the leaving group X can be conducted to obtain the compound of the Formula 2a.
For example, in case R1 is hydrogen, the compound of the Formula 2a can be obtained from the compound of the Formula 6 through ester-hydrolysis before the substitution with the leaving group X.
And, in case R1 and R3 are respectively different C1-C6 alkyl, the compound of the Formula 2a can be obtained from the compound of the Formula 6 through alkylation to substitute R3 with R1, or alternatively ester-hydrolysis and subsequent re-alkylation, before the substitution with the leaving group X.
Meanwhile, the compound of the Formula 3 may be preferably represented by the following Formula 3a wherein R2 is —CH2CH2OR2′.




wherein R2′ is hydrogen, C1-C6 linear or branched alkyl, C3-C6 cyclic alkyl, or C2-C6 alkenyl.
According to the process of the present invention, the compound of the above Formula 3a can be prepared in a more simplified process. More specifically, the compound of the Formula 3a can be prepared by the steps of: protecting amine group of piperidine ring in a compound of the following Formula 7 with a protection group to form a compound of the following Formula 8; N-alkylating the compound of the Formula 8 with a compound of the following Formula 9 to form a compound of the following Formula 10; and deprotecting the compound of the Formula 10:




wherein P is an amine protecting group; R2′ is hydrogen, C1-C6 linear or branched alkyl, C3-C6 cyclic alkyl, or C2-C6 alkenyl; and Y is a leaving group.
In the Formulas, the leaving group Y can be a common leaving group suitable for N-alkylation, for example, halogen, sulfinyloxy group or sulfonyloxy group.
In the process, the compound of the Formula 7 is commercially available.
Further, an amine protecting group (P) is introduced in the compound of the above Formula 7 in an organic solvent or aqueous solvent to form the compound of the above Formula 8. The amine protecting group P that can be used includes, without limitation, carbamate, amide, and silyl group, etc., and preferably carbamate.
The N-alkylation of the compound of the Formula 8 with the compound of the Formula 9 can be conducted in the presence of a base in an organic solvent. As the base, an inorganic base such as sodium hydroxide, potassium hydroxide, sodium C1-C6 alkoxide, potassium C1-C6 alkoxide, sodium carbonate, potassium carbonate, lithium carbonate, cesium carbonate, sodium bicarbonate, potassium bicarbonate, or potassium phosphate, or an organic base such as 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU), 1,4-diazabicyclo[2.2.2]octane (DABCO), 1,5-diazabicyclo[4.3.0]non-5-ene (DBN), pyridine, dimethylaminopyridine or triethylamine can be used. Preferably, an inorganic base such as sodium hydroxide, potassium hydroxide, sodium C1-C6 alkoxide, potassium C1-C6 alkoxide, sodium carbonate, potassium carbonate, lithium carbonate, cesium carbonate, sodium bicarbonate, potassium bicarbonate, or potassium phosphate can be used.
As explained, after the formation of the compound of the Formula 10, it is deprotected to prepare the compound of the Formula 3a. Commonly used amine deprotection method and conditions can be applied for the deprotection.
Meanwhile, It is pharmaceutically preferable that 2-methyl-2′-phenylpropionic acid derivative of the Formula 1 prepared by the above method is 2-[4-(2-{4-[1-(2-ethoxy-ethyl)-1H-benzoimidazol-2-yl]-piperidin-1-yl}ethyl)-phenyl]-2-methyl-propionic acid.
According to the preparation process of the 2-methyl-2′-phenylpropionic acid derivatives as explained, pharmaceutically useful 2-methyl-2′-phenylpropionic acid derivatives, which show excellent antihistamine activity, can be obtained with higher yield and purity in a more simplified process.
Accordingly, the process of the present invention is very efficient and appropriate for industrial scale production of the pharmaceutically useful 2-methyl-2′-phenylpropionic acid derivatives, and thus can raise pharmaceutical and industrial usefulness of the 2-methyl-2′-phenylpropionic acid derivatives.
The present invention is further explained in more detail with reference to the following examples. These examples, however, should not be interpreted as limiting the scope of the present invention in any manner.
EXAMPLE 1
Preparation of 2-[4-(2-hydroxy-ethyl)-phenyl]-2-methyl-propionic acid methylester
In a reaction vessel, 4-bromo phenethyl alcohol (2.0 g), Pd(dba)2 (0.11 g), t-Bu3P (0.08 g), ZnF2 (0.52 g), methyltrimethylsilyl dimethylketene acetal (2.6 g) and DMF (20 mL) were introduced, and the mixture was reacted at 80° C. for 18 hours. Distilled water (50 mL) and ethyl acetate (50 mL) were added thereto, and the mixture was stirred to separate layers. The separated organic layer was dehydrated with Na2SO4, and then condensed by filtration, and purified to obtain 2-[4-(2-hydroxy-ethyl)-phenyl]-2-methyl-propionic acid methylester (2.4 g, yield 100%).
1H-NMR, 400 MHz, CDCl3, ppm: δ 1.57 (s, 6H), 2.82-2.84 (t, 2H), 3.65 (s, 3H), 3.83-3.86 (t, 2H), 7.18-7.20 (d, 2H), 7.27-7.29 (d, 2H)
EXAMPLE 2
Preparation of 2-[4-(2-hydroxy-ethyl)-phenyl]-2-methyl-propionic acid methylester
In a reaction vessel, 4-bromo phenethyl alcohol (2 g), Pd2(dba)3 (0.18 g), t-Bu3P (0.16 g), ZnF2 (0.51 g), methyltrimethylsilyl dimethylketene acetal (2.6 g) and DMF (20 mL) were introduced, and the mixture was reacted at 80° C. for 18 hours. Distilled water (50 mL) and ethylacetate (50 mL) were added thereto, and the mixture was stirred to separate layers. The separated organic layer was dehydrated with Na2SO4, and then condensed by filtration, and purified to obtain 2-[4-(2-hydroxy-ethyl)-phenyl]-2-methyl-propionic acid methylester (2.2 g, yield 94%).
1H-NMR, 400 MHz, CDCl3, ppm: δ 1.57 (s, 6H), 2.82-2.84 (t, 2H), 3.65 (s, 3H), 3.83-3.86 (t, 2H), 7.18-7.20 (d, 2H), 7.27-7.29 (d, 2H)
EXAMPLE 3
Preparation of 2-[4-(2-hydroxy-ethyl)-phenyl]-2-methyl-propionic acid methylester
In a reaction vessel, 4-bromo phenethyl alcohol (5.0 g), hexamethyldisilizane (HMDS; 3.0 g), acetonitrile (25 mL) and ammoniumchloride (0.01 g) were introduced, and the mixture was stirred at room temperature for 1 hours or more. The mixture was condensed under reduced pressure, and dichloromethane (30 mL) and distilled water (20 mL) were added to separate layers. The separated organic layer was dehydrated with Na2SO4, and then, condensed by filtration. And then, Pd(dba)2 (0.29 g), t-Bu3P (0.20 g), ZnF2 (1.28 g), methyltrimethylsilyl dimethylketene acetal (2.2 g) and DMF (50 mL) were added thereto, and the mixture was reacted at 80° C. for 18 hours. Distilled water (75 mL), ethylacetate (75 mL) and 1N hydrochloric acid (9 mL) were added thereto, and the mixture was stirred to separate layers. The separated organic layer was dehydrated with Na2SO4, and then condensed by filtration, and purified to obtain 2-[4-(2-hydroxy-ethyl)-phenyl]-2-methyl-propionic acid methylester (5.2 g, yield 88%).
1H-NMR, 400 MHz, CDCl3, ppm: δ 1.57 (s, 6H), 2.82-2.84 (t, 2H), 3.65 (s, 3H), 3.83-3.86 (t, 2H), 7.18-7.20 (d, 2H), 7.27-7.29 (d, 2H)
EXAMPLE 4
Preparation of 2-[4-(2-hydroxy-ethyl)-phenyl]-2-methyl-propionic acid ethylester
In a reaction vessel, 4-bromo phenethyl alcohol (2.0 g), Pd(dba)2 (0.11 g), t-Bu3P (0.08 g), ZnF2 (0.52 g), ethyltrimethylsilyl dimethylketene acetal (2.7 g) and DMF (20 mL) were introduced, and the mixture was reacted at 80° C. for 18 hours. Distilled water (50 mL) and ethylacetate (50 mL) were added thereto, and the mixture was stirred to separate layers. The separated organic layer was dehydrated with Na2SO4, and then condensed by filtration, and purified to obtain 2-[4-(2-hydroxy-ethyl)-phenyl]-2-methyl-propionic acid ethylester (2.3 g, yield 92%).
1H-NMR, 400 MHz, CDCl3, ppm: δ 1.25 (t, 3H), 1.57 (s, 6H), 2.82-2.84 (t, 2H), 4.10-4.45 (q, 2H), 3.83-3.86 (t, 2H), 7.18-7.20 (d, 2H), 7.27-7.29 (d, 2H)
EXAMPLE 5
Preparation of 2-[4-(2-methanesulfonyloxy-ethyl)-phenyl]-2-methyl-propionic acid methylester
In a reaction vessel, 2-[4-(2-hydroxy-ethyl)-phenyl]-2-methyl-propionic acid methylester (2.2 g) prepared in the Examples 1-3, triethylamine (1.2 g), methanesulfonylchloride (1.25 g) and dichloromethane (20 mL) were introduced, and the mixture was reacted at room temperature for 2 hours. Distilled water (50 mL) was added thereto, and the mixture was stirred to separate layers. The separated organic layer was dehydrated Na2SO4, and then condensed by filtration to obtain 2-[4-(2-methanesulfonyloxy-ethyl)-phenyl]-2-methyl-propionic acid methylester (3.2 g, yield 100%).
1H-NMR, 400 MHz, CDCl3, ppm: δ 1.57 (s, 6H), 2.86 (s, 3H), 3.02 (t, 2H), 3.65 (s, 3H), 4.39-4.43 (t, 2H), 7.19-7.21 (d, 2H), 7.28-7.30 (d, 2H)
EXAMPLE 6
Preparation of 2-methyl-2-{4-[2-(toluene-4-sulfonyloxy)-ethyl]phenyl}-propionic acid methylester
In a reaction vessel, 2-[4-(2-hydroxy-ethyl)-phenyl]-2-methyl-propionic acid methylester (2 g) prepared in the Examples 1-3, triethylamine (1.1 g), p-toluenesulfonylchloride (1.9 g) and dichloromethane (20 mL) were introduced, and the mixture was reacted at room temperature for 1 hour. Distilled water (50 mL) was added thereto, and the mixture was stirred to separate layers. The separated organic layer was dehydrated with Na2SO4, and then condensed by filtration to obtain 2-methyl-2-{4-[2-(toluene-4-sulfonyloxy)-ethyl]-phenyl}-propionic acid methylester (3.0 g, yield 91%).
1H-NMR, 400 MHz, CDCl3, ppm: δ 1.57 (s, 6H), 2.44 (s, 3H), 2.93 (t, 2H), 3.65 (s, 3H), 4.18 (t, 2H), 7.05-7.07 (d, 2H), 7.19-7.21 (d, 2H), 7.27-7.30 (d, 2H), 7.69 (d, 2H)
EXAMPLE 7
Preparation of 2-[4-(2-chloro-ethyl)-phenyl]-2-methyl-propionic acid methylester
In a reaction vessel, 2-[4-(2-hydroxy-ethyl)-phenyl]-2-methyl-propionic acid methylester (1 g) prepared in the Examples 1-3, thionyl chloride (2.7 g) and dichloromethane (15 mL) were introduced, and the mixture was reacted at room temperature for 2 hours. Toluene (30 mL) was added, the mixture was condensed under reduced pressure, and the solvent was removed. Dichloromethane (50 mL) and sodium hydrogen carbonate solution (30 mL) were added thereto, and the mixture was stirred to separate layers. The separated organic layer was dehydrated with Na2SO4, and then condensed by filtration to obtain 2-[4-(2-chloro-ethyl)-phenyl]-2-methyl-propionic acid methylester (1.0 g, yield 92%).
1H-NMR, 400 MHz, CDCl3, ppm: δ 1.57 (s, 6H), 3.03-3.06 (t, 2H), 3.65 (s, 3H), 3.67-3.72 (t, 2H), 7.17-7.19 (d, 2H), 7.28-7.30 (d, 2H)
EXAMPLE 8
Preparation of 2-[4-(2-methanesulfonyloxy-ethyl)-phenyl]-2-methyl-propionic acid ethylester
In a reaction vessel, 2-[4-(2-hydroxy-ethyl)-phenyl]-2-methyl-propionic acid ethylester (2.2 g) prepared in the Example 4, triethylamine (1.2 g), methanesulfonylchloride (1.25 g) and dichloromethane (20 mL) were introduced, and the mixture was reacted at room temperature for 2 hours. Distilled water (50 mL) was added thereto, and the mixture was stirred to separate layers. The separated organic layer was dehydrated with Na2SO4, and then condensed by filtration to obtain 2-[4-(2-methanesulfonyloxy-ethyl)-phenyl]-2-methyl-propionic acid ethylester (3.2 g, yield 100%).
1H-NMR, 400 MHz, CDCl3, ppm: δ 1.57 (s, 6H), 2.86 (s, 3H), 3.02 (t, 2H), 3.65 (s, 3H), 4.39-4.43 (t, 2H), 7.19-7.21 (d, 2H), 7.28-7.30 (d, 2H)
EXAMPLE 9
Preparation of 2-{1-[4-(2-chloro-ethyl)-phenyl]-1-methyl-ethyl}-4,4-dimethyl-4,5-dihydro-oxazole
In a reaction vessel, 2-[4-(2-hydroxy-ethyl)-phenyl]-2-methyl-propionic acid ethylester (25 g) prepared in the Example 4 and dichloromethane (200 mL) were introduced, and then 2-amino-2-methyl-1-propanol (10.6 mL) was introduced, and potassium t-butoxide (11.9 g) was slowly introduced. After the reaction was completed, distilled water (100 mL) was introduced to separate a layer, and the organic layer was condensed under reduced pressure. To the condensed organic layer, acetonitrile (150 mL) was added, and thionyl chloride (16 mL) was added dropwise, and then, the mixture was stirred to complete the reaction. After the reaction was completed, the mixture was cooled and sodium hydroxide solution was slowly added thereto to control pH from 5 to 7. Then, an organic solvent was removed by condensation, distilled water (150 mL) and dichloromethane (150 mL) were added thereto to separate layers. The separate organic layer was condensed under reduced pressure to obtain 2-{1-[4-(2-chloro-ethyl)-phenyl]-1-methyl-ethyl}-4,4-dimethyl-4,5-dihydro-oxazole (22.5 g, yield 80%).
1H-NMR, 400 MHz, CDCl3, ppm: 1.27 (s, 6H), 1.57 (s, 6H), 3.07 (t, 2H), 3.69 (t, 2H), 3.85 (s, 2H), 7.18 (d, 2H), 7.32 (d, 2H)
EXAMPLE 10
Preparation of 4-(1H-benzoimidazol-2-yl)-piperidine-1-carboxylic acid tert-butyl ester
In a reaction vessel, benzoimidazole (1.1 g) and methylalcohol (8 mL) were introduced, and di tert-butyldicarbonate (1.2 g) was added thereto. The mixture was stirred at room temperature for about 3 hours, and then the mixture was condensed to obtain 4-(1H-benzoimidazol-2-yl)-piperidine-1-carboxylic acid tert-butyl ester (1.5 g, yield 91%).
1H-NMR, 400 MHz, CDCl3, ppm: 1.63 (s, 9H), 1.89 (m, 2H), 2.11 (d, 2H), 2.87 (t, 2H), 3.14 (m, 1H), 4.23 (s, 2H), 7.22-7.24 (m, 2H), 7.41 (s, 1H), 7.72 (s, 1H), 9.77 (s, 1H)
EXAMPLE 11
Preparation of 4-[1-(2-ethoxyethyl)-1H-benzoimidazol-2-yl]-piperidine-1-carboxylic acid tert-butyl ester
In a reaction vessel, 4-(1H-benzoimidazol-2-yl)-piperidine-1-carboxylic acid tert-butyl ester (1.5 g) prepared in the Example 10, toluene (10 mL) and potassium hydroxide (0.8 g) were introduced, and temperature of the mixture was controlled to 50° C. or more. To the mixture, ethoxyethanol methane sulfonate (1.3 g) was added, and then, it was stirred at the same temperature until the reaction was completed. After the reaction was completed, distilled water (10 mL) was added to separate layers, and then an organic layer was condensed to obtain 4-[1-(2-ethoxyethyl)-1H-benzoimidazol-2-yl]-piperidine-1-carboxylic acid tert-butyl ester (1.7 g, 91%).
1H-NMR, 400 MHz, CDCl3, ppm: 1.07 (t, 3H), 1.47 (s, 9H), 1.92 (m, 2H), 2.01 (s, 2H), 2.85 (s, 2H), 3.14 (m, 1H), 3.36 (m, 2H), 3.69 (t, 2H), 4.28 (t, 2H), 7.12-7.26 (m, 3H), 7.75 (t, 1H)
EXAMPLE 12
Preparation of 1-(2-ethoxyethyl)-2-piperidin-4-yl-1H-benzoimidazole
In a reaction vessel, 4-[1-(2-ethoxyethyl)-1H-benzoimidazol-2-yl]-piperidine-1-carboxylic acid tert-butyl ester (1.6 g) prepared in the Example 11, distilled water (15 mL) and hydrochloric acid (1.5 g) were introduced, and then the mixture was stirred to conduct deprotection. And then, sodium hydroxide solution (10 mL) and dichloromethane (20 mL) were added thereto. An organic layer was separated and condensed to obtain 1-(2-ethoxyethyl)-2-piperidin-4-yl-1H-benzoimidazole (1.2 g, 100%).
1H-NMR, 400 MHz, CDCl3, ppm: 1.05 (t, 3H), 2.09-2.23 (m, 4H), 3.17 (q, 2H), 3.24 (d, 1H), 3.39 (q, 2H), 3.51 (q, 3H), 3.77 (t, 2H), 4.51 (t, 2H), 7.27 (m, 2H), 7.52 (d, 1H), 7.62 (d, 1H)
EXAMPLE 13
Preparation of 2-(4-{2-[4-(1H-benzoimidazol-2-yl)-piperidin-1-yl]-ethyl}-phenyl)-2-methyl-propionic acid methylester
In a reaction vessel, 2-[4-(2-methanesulfonyloxy-ethyl)-phenyl]-2-methyl-propionic acid methylester (3.0 g) prepared in the Example 5, sodium carbonate (1.1 g), 2-(4-piperadinyl)-1H-benzoimidazole (3.0 g) and methylalcohol (30 mL) were introduced, and the mixture was reacted for 14 hours under reflux condition. Distilled water (50 mL) was added to crystallize. The crystals were filtered under reduced pressure, washed with distilled water, and dried to obtain 2-(4-{2-[4-(1H-benzoimidazol-2-yl)-piperidin-1-yl]-ethyl}-phenyl)-2-methyl-propionic acid methylester (3.4 g, yield 85%).
1H-NMR, 400 MHz, DMSO, ppm: 1.48 (s, 6H), 1.80-1.84 (m, 2H), 1.98-2.00 (m, 2H), 2.08-2.13 (t, 2H), 2.50-2.54 (m, 1H), 2.67-2.75 (t, 2H), 2.80-2.90 (m, 1H), 3.01-3.04 (d, 2H), 7.05-7.08 (m, 2H), 7.13-7.23 (d, 4H), 7.46 (s, 2H), 12.15 (s, 1H)
EXAMPLE 14
Preparation of 2-[4-(2-{4-[1-(2-ethoxy-ethyl)-1H-benzoimidazol-2-yl]-piperidin-1-yl}-ethyl)-phenyl]-2-methyl-propionic acid methylester
In a reaction vessel, 2-(4-{2-[4-(1H-benzoimidazol-2-yl)-piperidin-1-yl]-ethyl}-phenyl)-2-methyl-propionic acid methylester (0.5 g) prepared in the Example 13, potassium-t-butoxide (0.14 g), ethoxyethylmesylate (0.25 g) and DMF (20 mL) were introduced, and the mixture was reacted at 50° C. for 3 hours. Distilled water (50 mL) and ethylacetate (50 mL) were added thereto, and the mixture was stirred to separate a layer. Separated organic layer was washed with distilled water (50 mL), dehydrated, and condensed under reduced pressure to obtain 2-[4-(2-{4-[1-(2-ethoxy-ethyl)-1H-benzoimidazol-2-yl]-piperidin-1-yl}-ethyl)-phenyl]-2-methyl-propionic acid methylester (0.5 g, yield 85%, HPLC purity 98.5% or more).
1H-NMR, 400 MHz, DMSO, ppm: 0.98 (t, 3H), 1.48 (s, 6H), 1.87 (m, 4H), 2.05-2.20 (m, 2H), 2.50 (t, 2H), 2.73 (t, 2H), 3.04 (m, 3H), 3.30 (q, 2H), 3.58 (s, 3H), 3.65 (t, 2H), 4.39 (t, 2H), 7.00-7.30 (m, 6H), 7.40-7.60 (dd, 2H)
EXAMPLE 15
Preparation of 2-[4-(2-{4-[1-(2-ethoxy-ethyl)-1H-benzoimidazol-2-yl]-piperidin-1-yl}-ethyl)-phenyl]-2-methyl-propionic acid ethylester
In a reaction vessel, 2-[4-(2-methanesulfonyloxy-ethyl)-phenyl]-2-methyl-propionic acid ethylester (3.2 g) prepared in the Example 8, sodium carbonate (1.1 g), 1-(2-ethoxyethyl)-2-piperidin-4-yl-1H-benzoimidazole (3.0 g) prepared in the Example 12 and methylalcohol (30 mL) were introduced, and the mixture was reacted for 14 hours under reflux condition. Distilled water (50 mL) was added thereto, and the mixture was stirred and crystallized. The crystals were filtered under reduced pressure, and washed with distilled water, and then dried to obtain 2-[4-(2-{4-[1-(2-ethoxy-ethyl)-1H-benzoimidazol-2-yl]-piperidin-1-yl}-ethyl)-phenyl]-2-methyl-propionic acid ethylester (4.5 g, yield 90%, HPLC purity 98.5% or more).
1H-NMR, 400 MHz, CDCl3, ppm: 1.12 (t, 3H), 1.56 (s, 6H), 2.12 (m, 2H), 2.18 (m, 4H), 2.63 (q, 2H), 2.81 (q, 2H), 3.00 (m, 1H), 3.16 (d, 2H), 3.42 (q, 2H), 3.74 (t, 2H), 4.15 (q, 2H), 4.33 (t, 2H), 7.15-7.40 (m, 7H), 7.77 (q, 1H)
EXAMPLE 16
Preparation of 2-[4-(2-{4-[1-(2-ethoxy-ethyl)-1H-benzoimidazol-2-yl]-piperidin-1-yl}-ethyl)-phenyl]-2-methyl-propionic acid
In a reaction vessel, 2-[4-(2-{4-[1-(2-ethoxy-ethyl)-1H-benzoimidazol-2-yl]-piperidin-1-yl}-ethyl)-phenyl]-2-methyl-propionic acid methylester (2.3 g) prepared in the Example 14, sodium hydroxide (0.6 g) and ethylalcohol (13 mL) were introduced, and the mixture was reacted at 50-55° C. for 3 hours. Distilled water (20 mL) was added thereto, and acetic acid was added to control the pH to 7. Ethylacetate (50 mL) was added thereto, and the mixture was stirred to separate a layer. The separated organic layer was condensed under reduced pressure. And, butanol (9 mL) was added thereto, and dissolved by'heating. And then, it was cooled to precipitate crystals, and the crystals were filtered under reduced pressure to obtain 2-[4-(2-{4-[1-(2-ethoxy-ethyl)-1H-benzoimidazol-2-yl]-piperidin-1-yl}ethyl)-phenyl]-2-methyl-propionic acid (2.0 g, yield 90%, HPLC purity 99% or more).
1H-NMR, 400 MHz, CDCl3, ppm: 61.08 (t, 3H), 1.59 (s, 6H), 2.15-2.30 (m. 4H), 2.64-2.79 (m, 6H), 3.25 (s, 1H), 3.67 (q, 2H), 3.47 (m, 2H), 3.71 (t, 2H), 4.32 (t, 2H), 6.79 (d, 2H), 7.26 (m, 2H), 7.29-7.32 (m, 3H), 7.76 (t, 1H)
EXAMPLE 17
Preparation of 2-[4-(2-{4-[1-(2-ethoxy-ethyl)-1H-benzoimidazol-2-yl]-piperidin-1-yl}-ethyl)-phenyl]-2-methyl-propionic acid
In a reaction vessel, 2-[4-(2-{4-[1-(2-ethoxy-ethyl)-1H-benzoimidazol-2-yl]-piperidin-1-yl}-ethyl)-phenyl]-2-methyl-propionic acid ethylester (2.3 g) prepared in the Example 15, sodium hydroxide (0.6 g) and ethyl alcohol (13 mL) were introduced, and the mixture was reacted at 50-55° C. for 3 hours. Distilled water (20 mL) was added thereto, and acetic acid was added to control the pH to 7. Ethylacetate (50 mL) was added thereto, and the mixture was stirred to separate layers. The separated organic layer was condensed under reduced pressure. And, butanol (9 mL) was added and dissolved by heating. And then, it was cooled to precipitate crystals, and the crystals were filtered under reduced pressure to obtain 2-[4-(2-{4-[1-(2-ethoxy-ethyl)-1H-benzoimidazol-2-yl]-piperidin-1-yl}-ethyl)-phenyl]-2-methyl-propionic acid (1.84 g, yield 85%, HPLC purity 99% or more).
1H-NMR, 400 MHz, CDCl3, ppm: δ1.08 (t, 3H), 1.59 (s, 6H), 2.15-2.30 (m. 4H), 2.64-2.79 (m, 6H), 3.25 (s, 1H), 3.67 (q, 2H), 3.47 (m, 2H), 3.71 (t, 2H), 4.32 (t, 2H), 6.79 (d, 2H), 7.26 (m, 2H), 7.29-7.32 (m, 3H), 7.76 (t, 1H)
EXAMPLE 18
Preparation of 2-[1-(2-{4-[1-(4,4-dimethyl-4,5-dihydro-oxazol-2-yl)-1-methyl-ethyl]-phenyl}-ethyl)-piperidin-4-yl]-1-(2-ethoxy-ethyl)-1H-benzoimidazole
In a reaction vessel, 2-{1-[4-(2-chloro-ethyl)-phenyl]-1-methyl-ethyl}-4,4-dimethyl-4,5-dihydro-oxazole (1.9 g) prepared in the Example 9, sodium carbonate (0.8 g), 1-(2-ethoxyethyl)-2-piperidin-4-yl-1H-benzoimidazole (2.0 g) prepared in the Example 12 and methyl alcohol (20 mL) were introduced, and the mixture was reacted for 14 hours under reflux condition to obtain 2-[1-(2-{4-[1-(4,4-dimethyl-4,5-dihydro-oxazol-2-yl)-1-methyl-ethyl]-phenyl}-ethyl)-piperidin-4-yl]-1-(2-ethoxy-ethyl)-1H-benzoimidazole (4.4 g, yield 87%, HPLC purity 98% or more).
1H-NMR, 400 MHz, CDCl3, ppm: 1.10 (t, 3H), 1.27 (s, 6H), 1.57 (s, 6H), 1.92 (m, 2H), 2.10 (m, 4H), 2.60 (t, 2H), 2.80 (t, 2H), 3.01 (m, 1H), 3.10 (d, 2H), 3.41 (q, 2H), 3.70 (t, 2H), 3.90 (s, 2H), 4.30 (t, 2H), 7.10-7.32 (m, 7H), 7.8 (m, 1H)
EXAMPLE 19
Preparation of 2-[4-(2-{4-[1-(2-ethoxy-ethyl)-1H-benzoimidazol-2-yl]-piperidin-1-yl}-ethyl)-phenyl]-2-methyl-propionic acid
In a reaction vessel, 2-[1-(2-{4-[1-(4,4-dimethyl-4,5-dihydro-oxazol-2-yl)-1-methyl-ethyl]-phenyl}-ethyl)-piperidin-4-yl]-1-(2-ethoxy-ethyl)-1H-benzoimidazole (4.4 g) prepared in the Example 18 and hydrochloric acid aqueous solution (50 mL) were introduced, and the mixture was stirred with reflux. After the reaction was completed, sodium hydroxide solution was added to control the pH to 7. Ethylacetate (50 mL) was added to separate a layer, and the organic layer was condensed under reduced pressure. And, butanol (9 mL) was added and dissolved by heating. And then, it was cooled to precipitate crystals, and the crystals were filtered under reduced pressure to obtain 2-[4-(2-{4-[1-(2-ethoxy-ethyl)-1H-benzoimidazol-2-yl]-piperidin-1-yl}-ethyl)-phenyl]-2-methyl-propionic acid (3.43 g, yield 87%, HPLC purity 99% or more).
1H-NMR, 400 MHz, CDCl3, ppm: 61.08 (t, 3H), 1.59 (s, 6H), 2.15-2.30 (m. 4H), 2.64-2.79 (m, 6H), 3.25 (s, 1H), 3.67 (q, 2H), 3.47 (m, 2H), 3.71 (t, 2H), 4.32 (t, 2H), 6.79 (d, 2H), 7.26 (m, 2H), 7.29-7.32 (m, 3H), 7.76 (t, 1H)

## Claims

1. A process for preparing 2-methyl-2′-phenylpropionic acid derivatives of the following Formula 1, comprising the step of reacting a compound of the following Formula 2 with a compound of the following Formula 3:



wherein, A is oxygen or nitrogen;
when A is oxygen, R1 is hydrogen or C1-C6 linear or branched alkyl, and R2 is hydrogen or —CH2CH2OR2;
when A is nitrogen, R1 together with A forms a 5 to 7-membered ring unsubstituted or substituted with C1-C6 linear or branched alkyl and R2 is —CH2CH2OR2′;
R2′ is hydrogen, C1-C6 linear or branched alkyl, C3-C6 cyclic alkyl, or C2-C6 alkenyl; and
X is a leaving group.

2. The process according to claim 1, wherein the reaction is conducted in the presence of at least one base selected from the group consisting of sodium C1-C6 alkoxide, potassium C1-C6 alkoxide, sodium carbonate, potassium carbonate, lithium carbonate, cesium carbonate, sodium bicarbonate, potassium bicarbonate, potassium phosphate, 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU), 1,4-diazabicyclo[2.2.2]octane (DABCO), 1,5-diazabicyclo[4.3.0]non-5-ene (DBN), pyridine, dimethylaminopyridine and triethylamine.

3. The process according to claim 1, further comprising the step of ester-hydrolyzing the reaction product.

4. The process according to claim 1, further comprising the step of reacting the reaction product with a compound of the following Formula 9 when R2 in the reaction product is hydrogen:


YCH2CH2OR2′  [Formula 9]

wherein, R2′ is hydrogen, C1-C6 linear or branched alkyl, C3-C6 cyclic alkyl, or C2-C6 alkenyl; and Y is a leaving group.

5. The process according to claim 1, wherein 2-methyl-2′-phenylpropionic acid derivatives of the Formula 1 is 2-[4-(2-{4-[1-(2-ethoxy-ethyl)-1H-benzoimidazol-2-yl]-piperidin-1-yl}-ethyl)-phenyl]-2-methyl-propionic acid.

6. The process according to claim 1, wherein the compound of the Formula 2 is represented by the following Formula 2a:



wherein R1 is hydrogen or C1-C6 linear or branched alkyl, and X is a leaving group.

7. The process according to claim 6, further comprising the steps of reacting a compound of the following Formula 4 with a compound of the following Formula 5 to form a compound of the following Formula 6, and substituting —OB group of the Formula 6 with a leaving group X to form a compound of the Formula 2a:



wherein Hal is halogen, R1 is hydrogen or C1-C6 linear or branched alkyl, R3 is C1-C6 linear or branched alkyl, B is hydrogen or silyl protecting group, and X is a leaving group.

8. The process according to claim 7, further comprising the step of substituting R3 of the compound of the Formula 6 with R1 when R1 and R3 are different to each other.

9. The process according to claim 1, wherein the compound of the Formula 2 is represented by the following Formula 2b:



wherein X is a leaving group.

10. The process according to claim 1, wherein the compound of the Formula 3 is represented by the following Formula 3a:



wherein, R2′ is hydrogen, C1-C6 linear or branched alkyl, C3-C6 cyclic alkyl, or C2-C6 alkenyl.

11. The process according to claim 10, further comprising the steps of protecting amine group of piperidine ring in a compound of the following Formula 7 with a protection group to form a compound of the following Formula 8, N-alkylating the compound of the Formula 8 with a compound of the following Formula 9 to form a compound of the following Formula 10; and deprotecting the compound of the Formula 10 to form a compound of the Formula 3a:



wherein P is an amine protecting group; R2′ is hydrogen, C1-C6 linear or branched alkyl, C3-C6 cyclic alkyl, or C2-C6 alkenyl; and Y is a leaving group.

12. The process according to claim 11, wherein the reaction of the compound of the Formula 8 with the compound of the Formula 9 is conducted in the presence of at least one base selected from the group consisting of sodium hydroxide, potassium hydroxide, sodium C1-C6 alkoxide, potassium C1-C6 alkoxide, sodium carbonate, potassium carbonate, lithium carbonate, cesium carbonate, sodium bicarbonate, potassium bicarbonate, potassium phosphate, 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU), 1,4-diazabicyclo[2.2.2]octane (DABCO), 1,5-diazabicyclo[4.3.0]non-5-ene (DBN), pyridine, dimethylaminopyridine and triethylamine.