# Patent

## Title
Monolithic electrophoresis gel system

## Abstract

Apparatus, systems and methods for performing gel electrophoresis using a monolithic electrophoresis unit that at least includes first and second buffer chambers containing buffer solution and a gel chamber containing a pre-cast gel, whereby all of these chambers are integrated into a pre-fabricated single unit that is ready for use. In performing gel electrophoresis, a top seal of this monolithic electrophoresis unit is removed, followed by removing a gel chamber seal from over the gel chamber, such that, buffer solution contacts the pre-cast gel within such gel chamber. Target samples are loaded into the gel chamber for contact with the pre-cast gel, a reusable lid is attached to the top surface of the monolithic electrophoresis unit, and an electrical connection is provided through the reusable lid into the monolithic electrophoresis unit for performing electrophoresis on the pre-cast gel.

## Background
BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention is directed to gel electrophoresis, and in particular, to a pre-cast vertical monolithic gel electrophoresis system and an electrophoresis method using the present monolithic system.
2. Description of Related Art
Gel electrophoresis is known for separating and purifying protein, peptide DNA, RNA, and a variety of electrically charged macro molecules for the study thereof, or as a preparative step for subsequent analytical procedures. For instance, gel electrophoresis is often used to separate and purify molecules for protein purity verification, protein identification, subsequent DNA sequencing, blotting procedures, mass spectrometry, PCR, RFLP, cloning, or other known techniques for further characterization.
In vertical electrophoresis, a gel matrix is placed in a buffer-filled electrophoresis tank such that, the gel is submerged just beneath the buffer surface vertically. The gel matrix is of a material having both a composition and porosity suitable for the specific molecular weight, size and composition of the target molecules to both contain and separate these target molecules. The gel matrix also has wells for receiving alloquots of the target sample to be tested. The electrophoresis tank has cathode and anode terminals on opposite sides of the gel matrix, such that, once the target samples reside in the gel matrix, an electric current is applied to the matrix for generating an electric field. This electric field separates the charged molecules suspended in the gel matrix, whereby negatively charged molecules move in bands through the matrix at different rates toward the anode, while positively charged molecules move in bands through the matrix also at different rates toward the cathode.
In a conventional electrophoresis assembly, the electrophoresis box is composed of two separate flat plates separated by spacers for holding a gel. The electrophoresis apparatus is assembled by providing a gel, either vertically or horizontally, between these flat plates. This gel may be a pre-cast (i.e., prefabricated) gel that is removed from its packaging and placed between the two flat plates, or it may be mixed by the user assembling the electrophoresis apparatus, poured into a gel mold between the plates and then allowed to set for several hours to form a gel. The electrophoresis tank also includes two reservoirs for containing a buffer solution, whereby each reservoir is on opposite sides of the gel and has an electrode therein. Once the gel is provided between the plates, a buffer solution is poured into each of these reservoirs so that the buffer covers the gel and the electrodes reside within the buffer. With the samples to be tested residing in wells of the gel, an electric current is then applied to the assembly via the electrodes to generate an electric field across the gel for separating the charged molecules in such gel. The gel is then removed for subsequent analytical procedures, and the equipment must be thoroughly washed for reuse.
However, these conventional gel electrophoresis assemblies and approaches are lengthy, and require a significant amount of processing steps and skill in preparing the gel, assembling the apparatus and running the gel. These approaches also require a large amount of buffer to run the gel, typically greater than 750 ml, and are prone to leakage due to the apparatus being improperly assembled. Additionally, these conventional gel electrophoresis systems that include the buffer tank, various clamps and gel holders, buffer dam and tank cover, and supplies for running gels are expensive, bulky and require washing and maintenance thereof after performing an electrophoresis run.
Accordingly, a need continues to exist for improved electrophoresis assemblies that are easy to use, require minimal processing and handling steps, are structurally and mechanically stable, time efficient, and are low cost.
SUMMARY OF THE INVENTION
Bearing in mind the problems and deficiencies of the prior art, it is therefore an object of the present invention to provide a prefabricated, monolithic gel electrophoresis apparatus that is ready to use for electrophoresis.
It is another object of the present invention to provide an electrophoresis method using a prefabricated, monolithic gel electrophoresis apparatus.
A further object of the invention is to provide a prefabricated monolithic gel electrophoresis apparatus that can be used directly without assembly or modification thereto.
Another object of the present invention is to provide a prefabricated, monolithic gel electrophoresis apparatus that is inexpensive and requires minimal handling and preparation for use in electrophoresis.
It is yet another object of the present invention to provide a prefabricated monolithic electrophoresis system that requires no washing, maintaining and storing of a buffer tank and various other components (e.g., clamps, gel holders, etc.)
Still other objects and advantages of the invention will in part be obvious and will in part be apparent from the specification.
The above and other objects, which will be apparent to those skilled in the art, are achieved in the present invention which is directed to one-piece electrophoresis apparatuses for use in performing gel electrophoresis. These one-piece electrophoresis apparatuses at least include first and second buffer chambers containing buffer solution with a gel chamber there-between. The gel chamber contains a pre-cast gel that at least includes a separating gel matrix. These gel chamber and first and second buffer chambers are integrated with each other into a single unit that comprises a molded monolithic electrophoresis unit that is ready for use.
In another aspect, the invention is directed to systems for use in performing gel electrophoresis. These systems at least include a reusable lid and a pre-fabricated monolithic electrophoresis unit. This unit includes an anode buffer chamber and a cathode buffer chamber both containing buffer solution, and a gel chamber. The gel chamber is integrated with and resides between the anode and cathode buffer chambers, and contains a pre-cast gel. This entire pre-fabricated monolithic electrophoresis unit is ready for use in performing gel electrophoresis. The system also includes an electrical connection between the reusable lid and the pre-fabricated monolithic electrophoresis unit for performing the gel electrophoresis.
In still another aspect, the invention is directed to methods for performing gel electrophoresis. These methods at least include providing a monolithic electrophoresis unit that has a unit seal on a top surface thereof. This monolithic electrophoresis unit includes anode and cathode buffer chambers containing buffer solution and a gel chamber having a gel chamber seal. The gel chamber is integrated with and resides between the anode and cathode buffer chambers and contains a pre-cast gel. The methods further include removing both the unit seal and the gel chamber seal. A target sample is then loaded into the monolithic electrophoresis unit, followed by attaching a reusable lid to the top surface of such unit. Electrophoresis is then performed on the pre-cast gel by providing an electrical connection through the reusable lid into the monolithic electrophoresis unit.



BRIEF DESCRIPTION OF THE DRAWINGS
The features of the invention believed to be novel and the elements characteristic of the invention are set forth with particularity in the appended claims. The figures are for illustration purposes only and are not drawn to scale. The invention itself, however, both as to organization and method of operation, may best be understood by reference to the detailed description which follows taken in conjunction with the accompanying drawings in which:
FIG. 1A is a perspective view of a prefabricated, monolithic gel electrophoresis unit and system according to the present invention.
FIG. 1B is a cross-sectional view of the pre-fabricated, monolithic gel electrophoresis unit shown in FIG. 1A.
FIGS. 2A-E are perspective views showing one or more embodiments of a reusable lid in accordance with the invention.
FIGS. 3A-F are cross-sectional views of alternate prefabricated, monolithic gel electrophoresis units in accordance with the invention.
FIG. 4A-B are perspective views showing pre-cast well indicators and/or sample wells in the buffer chambers and/or the separating gel matrix in accordance with the invention.
FIG. 5 is a perspective view of a method of performing an electrophoresis run using the prefabricated, monolithic gel electrophoresis unit and system of the invention.
FIG. 6 is a cross-sectional view showing the current flow in the monolithic gel electrophoresis system of FIG. 4.
FIG. 7 is a perspective view showing the disassembly of the monolithic gel electrophoresis system of FIG. 4.



DESCRIPTION OF THE PREFERRED EMBODIMENT(S)
In describing the preferred embodiment of the present invention, reference will be made herein to FIGS. A-7 of the drawings in which like numerals refer to like features of the invention.
The present invention is directed to apparatus, systems and methods for performing gel electrophoresis using a monolithic (i.e., one-piece), prefabricated gel electrophoresis assembly that includes a gel matrix and buffer, whereby the assembly is ready to use for electrophoresis. The present apparatus and systems are compact, all-in-one units that integrate the gel and buffer chambers into a single, molded unit that is structurally stable and prevents leakage. The entire system, as described in detail below, includes only two components, in particular, a reusable lid having electrodes and a disposable pre-fabricated electrophoreses unit having gel chamber(s) integrated with buffer chamber(s). Preferably, the system is a pre-cast vertical monolithic gel electrophoresis system.
Referring now to FIGS. 1A-B, one embodiment of a gel electrophoresis assembly 100 is shown according to the present invention. The assembly 100 includes a reusable lid 2 having at least two electrodes 4, 6 and a pre-fabricated, monolithic gel electrophoresis unit 10. As shown, the lid 2 includes at least a pair of openings for receiving a first connector 7 that is electrically connected to a power source and a second connector 7 that is also electrically connected to the power source. Optionally, as shown in FIG. 1A, the lid may include a moveable window 95 on a top surface of the lid, whereby this window 95 is movable from an opened to closed position, and vice versa. The window 95 is beneficial in the event additional buffer or target sample needs to be added inside the unit 10 during an electrophoresis run. Alternatively, referring to FIG. 2E, a liquid crystal display 96 may reside at the top surface of the lid 2. This liquid crystal display 96 is preferably thermally connected to one or more of the buffer chambers for measuring and providing the temperature of the running buffer during an electrophoresis run.
FIGS. 2A-E show one or more embodiments of the present reusable lid 2. As is shown, the lid 2 is constructed of a rigid material, such as a rigid plastic or glass material (e.g., acrylic plastic). The lid 2 may include a seal 3 located at or near the bottom surface thereof for providing a secure and leak-proof fit between the lid 2 and unit 10 once assembled together. Attached to the lid are downwardly extending rods 1 for protecting an electrode wire 8 therein from damage and distortion during use, handling and storage of the lid. The electrode wire 8 is composed of an electrically conductive material including, but not limited to, platinum, titanium, platinized titanium, and the like. The lid 2 includes a pair of anode electrodes 4 for providing a negative electrical charge to buffer residing within a first chamber of the electrophoresis unit 10, and a pair of cathode electrodes 6 for providing a positive electrical charge to buffer residing within a second chamber of unit 10. While the drawings show the pairs of electrodes 4, 6 in electrical communication with one another via conductive wires, it should be appreciated that a single anode and cathode may be used in each buffer chamber, or even additional connectors 7 may be used to provide the corresponding electrical charge from outside the assembly 100.
Referring to FIG. 2D, each of the anode and cathode electrodes 4, 6 include the conductive electrode wire 8 that extends down from the respective connector 7 (not shown), through a first protective rod 1 of the lid and across to an adjacent protective rod 1 of the lid. These protective rods 1 may be molded as part of the lid during fabrication thereof. As an alternative, the rods may be securely attached to the lid as separate components, such that, the electrodes 4, 6 and/or connectors 7 are removable and replaceable. The protective rods 1 preferably each have a notch at an end thereof for receiving and holding the electrode wire 8 in position.
The lid may also include a protective bar 5 of a rigid material that encases the wire 8 as it extends from one rod to an adjacent rod for the protection of such wire 8. This protective bar 5 may also be molded as part of the lid, or alternatively, it may be securely attached to the lid as a separate component whereby this protective bar 5 is received, held and secured by the notches at the ends of the rods 1. Referring to FIG. 2C, the lid may further include a notched guard 9 for receiving and protecting the protective bar 5 (if present) and/or the electrode wire 8 from damage, distortion and/or breakage. That is, the notch of guard 9 protects either the wire 8 itself, or if the wire 8 is encased by the protective bar 5, then the guard 9 protects both the bar 5 and the encased wire 8. Like rods 1 and bar 5, this notched guard 9 may also be molded as part of the lid or it may be a separate component that is securely attached to the lid. The notched guard 9 is attached to an inside surface of the lid and may either extend partially between the electrodes 4, 6; or it may extend entirely between the electrodes and have a first downwardly extending sidewall attached between and directly to the pair of anode electrodes 4 and a second downwardly extending sidewall attached between and directly to the pair of cathode electrodes 6.
The lid 2 is preferably fabricated with an attachment control(s) that allows the lid to be attached to the pre-fabricated, monolithic gel electrophoresis unit 10 in one direction only so that the positive anode electrodes 4 are aligned with an anode buffer chamber 14 while the negative cathode electrodes 6 are aligned with a cathode buffer chamber 16 of unit 10. In doing so, both the lid 2 and the unit 10 may each include attachment controls of an alignment indicator (e.g., color coding, symbols, numbers, letters, etc.) to ensure that the lid 2 is correctly attached to unit 10. Alternatively, the lid 2, unit 10, or lid 2 and unit 10 together, may be fabricated with attachment controls of mating patterns or design(s) (e.g., notches, lips, edges, rims, grooves, male-female matings, etc.) that prohibit the lid 2 from being incorrectly attached to the unit 10.
In accordance with embodiments of the invention, the pre-fabricated, monolithic gel electrophoresis unit 10 is a molded one-piece electrophoresis box of a rigid plastic or glass material that includes an open top surface and a bottom surface 11 connected to opposing outer walls 12 and opposing sidewalls 13. It should be appreciated that the outer walls, sidewalls and bottom surface each have a thickness that provides the box with sufficient rigidity to withstand handling, transport, and processing procedures. For instance, wherein the box is composed of a rigid plastic material, the thickness of the outer walls, sidewalls and bottom surface may have a thickness of about 1 mm or greater.
As shown in FIG. 1B, the interior of the electrophoresis box includes at least two opposed upstanding interior walls 17 and 18 that are parallel with and spaced apart from the opposing outer walls 12 to form at least three interior chambers within the pre-cast or molded electrophoresis box. These interior chambers include an anode buffer chamber 14, a cathode buffer chamber 16 and a gel chamber 15 between the anode and cathode buffer chambers 14, 16.
The first interior wall 17 is attached to the opposing sidewalls 13 and extends to the open top of the one-piece electrophoresis box to both separate the gel chamber 15 from the anode buffer chamber 14, and to electrically isolate the anode buffer chamber 14 from the cathode buffer chamber 16. The first interior wall 17 also has an opening 19 at the bottom end thereof, which resides at the bottom surface 11 of the box. This opening 19 allows electrical communication between the anode buffer chamber 14 and the gel chamber 15. The second interior wall 18 is also attached to the opposing sidewalls 13 and separates the gel chamber 15 from the cathode buffer chamber 16. This second interior wall 18 has a bottom end in contact with the bottom surface 11 of the box and a top end that resides below the open top of the one-piece electrophoresis box, such that, buffer solution 50 is allowed to contact both the top surface of the gel chamber 15 and the cathode buffer chamber 16 for allowing electrical communication between chambers 15, 16.
The present pre-fabricated, monolithic gel electrophoresis unit 10 also includes a separating gel matrix 20 inside the gel chamber 15 and a buffer solution 50 within both the anode and cathode buffer chambers 14, 16. The buffer 50 also resides partially within gel chamber 15 during operation, at least over a top surface of the separating gel matrix 20, to both cover the matrix 20 and enable the electrical connection between the cathode buffer chamber 16 and the gel chamber 15. In one or more embodiments of the invention, the separating gel matrix 20 may be a polyacrylamide gel with a concentration between 2% to 30%, in a Tris[tris(hydroxymethyl)aminomethane)]-Citrate buffer pH 6.8, whereby the concentrations of tris and citrate are about 200 mM. The running buffer is Tris and Hepes((4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) pH 7.8, with a concentration of about 100 mM.
Referring to FIGS. 3A-B, a side view of the present pre-fabricated, monolithic gel electrophoresis unit 10 is shown in accordance with one or more embodiments of the invention. The gel matrix 20 is composed of a material having a composition and porosity chosen based on the specific weight and composition of the target to be analyzed and subjected to electrophoresis. That is, the material of the separating gel matrix 20 may have varying compositions, concentrations of constituent chemicals, porosities, strengths, optical transparency, and the like, all of which depend on the target sample. For instance, the separating gel matrix 20 may include, but is not limited to, polyacrylamide gels, argrose gels, or any other gel suitable for use in gel electrophoresis. Likewise, the buffer 50 provided in the present pre-fabricated, monolithic gel electrophoresis unit 10 varies depending upon the sample being analyzed and subjected to electrophoresis, as well as depending upon the composition and characteristics of the gel matrix 20 or gel matrices residing within the unit 10.
Regardless of the compositions of the gel matrix 20 and the buffer 50, an essential feature of one or more embodiments of the invention is that both the gel matrix 20 and the buffer 50 are pre-cast and preloaded into the present monolithic gel electrophoresis unit 10. In this manner, there is no need to prepare and cast a gel matrix prior to performing an electrophoresis run, and no need to make and pour a buffer solution into an electrophoresis tank or chamber, thereby preventing spillage of buffer solution.
FIG. 3A shows a pre-fabricated, monolithic gel electrophoresis unit 10 of the invention prior to an electrophoresis run. The unit 10 includes buffer 20 in both the anode and cathode buffer chambers 14, 16, along with a separating gel matrix 20 in the gel chamber 15, whereby this unit 10 is ready for use and does not require any assembling or fabricating of a gel or buffer prior to performing an electrophoresis run.
In one or more embodiments, the buffer 50 is pre-filled in both the anode and cathode buffer chambers to a volume marker 52 that resides on the electrophoresis box. This volume marker 52 may reside on the outside or inside surface of the outer walls 12 (e.g., a color indicating fill line), or it may be etched directly into at least one or both of the outer walls 12. As an alternative to having the buffer pre-loaded to the marker 52, the buffer may be a concentrated buffer solution or powder that is reconstituted to normal strength by adding a liquid, preferably water, to the volume marker 52 prior to performing electrophoresis.
In the present monolithic gel electrophoresis units 10, the separating gel matrix 20 resides entirely inside the gel chamber 15 for separating target molecules. Optionally, a stacking gel 22 may reside over the separating gel matrix 20. The stacking gel 22 is preferably of a different concentration, or even a different composition, than the separating gel matrix 20, and is used to form a number of sample wells over the separating gel matrix 20, which are discussed further below. At the bottom of the separating gel matrix 20 resides a base sealing gel 24. This base sealing gel 24 is also of a different concentration, or even a different composition, than the separating gel matrix 20. In accordance with one or more embodiments of the invention, the stacking gel 22 and base sealing gel 24 are preferably both of a different concentration, or a different composition, than the separating gel matrix 20 for allowing easy and effective removal of the separating gel matrix 20 from the unit 10 while avoiding damage to such matrix 20.
The base sealing gel 24 is used to seal the opening(s) 19 between the gel chamber(s) and the anode buffer chamber(s) 14. In so doing, the base sealing gel 24 resides in both the gel chamber 15 and the anode buffer chamber 14, such that, it fills opening 19 at the bottom of the first interior wall 17. The sealing gel 24 prevents the separating gel matrix 20 (or matrices) from entering anode buffer chamber(s) 14 during its polymerization, and prevents contact of the separating gel matrix (matrices) with buffer during transportation and storage. As such, the base sealing gel 24 is poured into unit 10 prior to the separating gel matrix 20 so that the separating gel 20 does not flow to the anode buffer chamber 14 before it is polymerized.
Preferably, as shown in FIGS. 3A-B, an internal beveled notch 30 resides at the bottom surface 11 of the unit, and extends along the length of the bottom 11 in the anode buffer chamber 14. This internal beveled notch 30 aids in retaining the base sealing gel 24 inside the gel chamber 15 and reduces the amount of base sealing gel 24 needed to seal and isolate the separating gel matrix 20. Alternatively, as shown in FIG. 1B, the bottom surface 11 of the anode buffer chamber 14 is planar (i.e., does not have an internal beveled notch 30), such that, the base sealing gel 24 resides at the bottom of both the anode buffer chamber 14 and the gel chamber 15 as denoted by the dashing line.
It should be appreciated that the present prefabricated monolithic gel electrophoresis units 10 are not limited to having a single gel chamber 15 with a single separating gel matrix 20 therein. One or more embodiments of the invention are directed to prefabricated monolithic gel electrophoresis units 10 having various designs that include a plurality of gel chambers 15 each having a separating gel matrix 20 therein. That is, while not departing from the scope of the invention, the prefabricated units 10 may have a single pre-cast gel matrix 20 therein for running a single gel matrix, or alternatively, the prefabricated units 10 may have two or more pre-cast gel matrices 20 residing therein for simultaneously running a plurality of gel matrices.
FIGS. 3C-F show one or more embodiments of multi-gel chambers 15 and/or multi-separating gel matrix (matrices) 20 prefabricated into the monolithic gel electrophoresis units 10 of the invention. Again, multiple gels 20 may be run simultaneously within a single unit 10. Referring to the embodiment of FIG. 3C, a first separating gel matrix 20 resides in a first gel chamber, while a second separating gel matrix 20 resides in a second gel chamber. These first and second separating gels in each of the gel chambers both reside within the cathode buffer chamber 16, which in turn, now resides between a pair of anode buffer chambers 14, 14. In this aspect, all three buffer chambers 14, 16, 14 are filled with buffer and are separated from one another by a pair of interior walls 17, with the gel chambers 15 being separated from one another within the cathode buffer chamber by a pair of interior walls 18. A bottom or interior wall 23 resides at the bottom of this buffer chamber 16 to separate the buffer therein from the base sealing gel 24. It should be appreciated that the number of interior walls 17, 18 inside units 10 correspond to the number of buffer chambers 16 and the number of gel chambers 15 within the cathode buffer chamber 16.
Each anode buffer chamber 14, 14 may optionally include an internal beveled notch 30 at the bottom surface 11 of the unit 10 for both retaining base sealing gel 24 that resides under each of the separating gel matrices 20 and reducing the amount of such base sealing gel 24 required to fill this region of the unit 10. Again, the base sealing gel 24 prevents entering of separating gel 20 into chamber 14 and prevents contact between the separating gel matrix 20 and the buffer 50, and is composed of a material or has a composition that allows it to be easily removed from the separating gel matrix 20. In this manner, after completion of the electrophoresis run, the gel matrix 20 can be easily separated from the unit 10 for further analytical processing.
As discussed above, during the electrophoresis run, a portion of the base sealing gel 24 is in physical contact with the buffer in each of the anode buffer chambers 14, 14 to allow electrical communication between each separating gel matrix 20. Since these two separating gel matrices 20 are connected through openings 19 to the two anode chambers 14, such separating gel matrices 20 have the same voltage. This is beneficial in ensuring that the target samples within each of the gel matrices 20 are run at the same rate.
Referring to FIGS. 3D-F, it should be appreciated that there are various embodiments of a multi-gel chamber design that may be employed and prefabricated into the present units 10. For instance, referring to FIG. 3D, two or more separating gel matrices 20, 20′ may each reside in separate cathode buffer chambers 16, 16′, which in turn are separated from one another by an anode buffer chamber 14, whereby the current flows from the cathode buffer chambers 16, 16′, through the gel matrices 20, 20′ and into the anode buffer chamber 14. Referring to FIG. 3E, a single cathode buffer chamber 16 may have two or more separating gel matrices 20, 20′ therein that are adjacent one another and separated by an interior wall of the unit, whereby the current flows simultaneously through these two gel matrices, through the base sealing gel 24 and into the anode buffer chamber 14. As another alternative embodiment, as shown in FIG. 3F, the unit 10 may have two or more separating gel matrices 20, 20′ in a single cathode buffer chamber 16, whereby these gel matrices are separated by interior walls and have buffer solution residing therebetween. The current flows simultaneously through these two gel matrices, through the base sealing gel 24 and into the anode buffer chamber 14. It should be appreciated that many designs and configurations of electrophoresis units 10 having two or more separating gel matrices 20 therein may be implemented in accordance with the various embodiments of the invention.
Referring again to FIGS. 3A-B, prior to performing an electrophoresis run, a seal 45 resides at the top of the gel chamber 15 to isolate and prevent contact between the separating gel matrix 20 (and optionally the stacking gel 22 if present) and the buffer 50 residing in the cathode buffer chamber 16. As shown in FIG. 3A, this seal 45 may be a removable tape seal that resides along the entire length of the top of the gel chamber 15. A bevel 47 may reside at the top of the gel chamber 15 to allow and enhance adhesion of the tape seal to the top of the gel chamber.
Alternatively, as is shown in the cross section view of FIG. 3B along line a-a′, the seal 45 may be a removable gel comb 45′ having a beveled top surface for easy and efficient removal thereof. A sealing ring made from rubber can be placed on the top of 18 to further prevent leaking. The gel comb 45′ has a plurality of teeth that extend from the beveled top surface into the gel matrix 20 to form a plurality of wells 60 in the gel matrix. It should be appreciated that the gel comb 45′ may be provided with any number of downwardly extending teeth to fabricate a corresponding number of wells desired in the resultant unit 10. Since the gel 20 is pre-cast into the unit 10 along with the corresponding number of wells, the need to make a gel and fabricate wells therein is avoided. Once an electrophoresis run is to be performed, the seal 45 is removed to reveal the plurality of wells 60 extending into the gel matrix 20, which are to be used for sample loading.
As another embodiment of the invention, rather than using a gel comb 45 to provide a plurality of wells 60 in the gel matrix 20, either the first or second interior walls 17, 18 may be provided with a plurality of wells 60 molded directly into an inner surface of such walls 17, 18 as shown in FIGS. 4A and 7. That is, the side of interior wall 17 or 18 that is in direct contact with the gel matrix 20 is fabricated in a manner so that it has a plurality of wells 60 built directly into such wall. These wells 60 reside directly over and in contact with the below separating gel matrix 20 for easily loading the target sample onto or into the gel matrix 20. As another alternative, the walls 17, 18 may each be provided with partial well portions, such that, upon completion of the molded unit 10, entire wells 60 are formed directly over the gel matrix. Additionally, each well 60 built into wall 17 and/or wall 18 may include a marking or color indicator on at least a portion thereof so that the wells are easily distinguished from the rest of unit 10 for loading samples therein.
The top surface of the pre-fabricated, monolithic gel electrophoresis unit 10 is also sealed with a seal 40 prior to performing electrophoresis. This seal 40 is preferably an easily removable layer that prevents leakage of the buffer 50 residing inside the unit 10 during transport, handling and storage of such unit prior to the use thereof. For instance, the seal 40 may include, but is not limited to, a easily removable film, tape, glued layer, adhesive layer, and the like. Again, during storage of the monolithic gel electrophoresis unit 10 both seals 40 and 45 tightly seal, respectively, the entire unit 10 and the gel chamber 15 so as to prevent leakage of any buffer from the unit itself and/or prevent contact between the buffer 50 and the separating gel matrix 20 (and optionally the stacking gel 22). Further, the unit 10 is preferably transported and stored in an inverted position (i.e., upside down) to prevent contact between the buffer 50 and base sealing gel 24 residing in the anode buffer chamber 14 since the buffer 50 may degrade the base sealing gel 24.
Referring to FIGS. 4A-B, either the separating gel matrix 20 or one of interior walls 17, 18 may further be provided with well indicators 65 comprising, for example, numbers, letters, symbols, etc. Preferably, the well indicators 65 are sequential numbers that reside about 3 mm above the bottom surface of the separating gel matrix 20, wall 17 or wall 18. As such, wall 17 or wall 18 may be provided with one or both of the plurality of wells 60 and/or the well indicators 65. The well indicators 65 preferably correspond to the number of wells 60 (i.e., same number of wells 60 and well indicators 65) for identifying each corresponding well 60. In embodiments where the well indicators 65 reside in or on the separating gel matrix 20, such well indicators may reside directly inside the gel matrix 20 spaced apart to define the individual wells 60 or they may be fabricated on an outside of the gel matrix 20 to identify each well 60. These well indicators 65 may be dyed well indicators fabricated inside or on an outside of the separating gel matrix, separate indicator components (e.g., plastic number pieces) set inside or on an outside of the gel matrix, indicators residing along a strip of material that is set inside or on an outside of the gel matrix, and the like. Alternatively, when the well indicators 65 reside in or on wall 17 or wall 18 these well indicators 65 may be raised above the surface of the wall (i.e., embossed) to form inset (i.e., engraved) well indicators in the separating gel matrix, they may be engraved in the wall to form embossed well indicators in the separating gel matrix, they may be engraved, embossed or planar surface dyed well indicators that are transposed into or onto the separating gel matrix, and the like.
FIG. 5 shows one embodiment of performing electrophoresis in accordance with the invention using the present pre-fabricated, monolithic gel electrophoresis unit 10. Once a pre-fabricated, monolithic gel electrophoresis unit 10 of the invention has been selected, the unit 10 is provided in its upright position and the seal 40 is removed from the top surface of the unit. For instance, wherein the seal 40 is a layer of removable tape, this tape layer is peeled back and removed from the unit 10. The gel chamber seal 45 that is covering and protecting the gel chamber 15 and the gels therein is also removed from the surface of this gel chamber 15. Again, the gel chamber seal 45 may be a removable layer (e.g., removable tape) or it may be a gel comb 45 that is removed to reveal a plurality of wells 60 residing over the separating gel matrix 20 and between adjacent sections of stacking gel 22.
Once the seals 40, 45 are removed from the unit, buffer 50 fills any empty regions inside the gel chamber. In so doing, the pre-loaded buffer may fill empty regions of the gel chamber, or if the buffer is in concentrated form, once it is reconstituted, the buffer fills such empty regions inside the gel chamber as shown in FIG. 3C. Target samples 80 to be tested are then loaded into the wells 60 residing inside the gel chamber(s) 15 of unit 10. Again, these wells 60 may reside in the stacking gel 22, which resides directly over the separating gel 20, or they may reside in interior wall 17 and/or interior wall 18. Regardless, each well 60 is preferably aligned with a corresponding well indicator 65 (which resides inside or outside of either the separating gel 20, interior wall 17 and/or interior wall 18), whereby each well 60 resides directly over the separating gel 20 to reveal top surface portions of the separating gel 20. Since the target samples are generally denser than the buffer, these target samples fall to the bottom of the well. It should be appreciated that the volume of the target samples will vary depending upon the well size.
After the target samples 80 are loaded into the wells, the lid 2 is provided over the top surface of unit 10 to form a gel electrophoresis assembly 100 of the invention. Again, the lid 2 is preferably designed so that it can be provided over the unit 10 in only one position so that the anode electrodes 4 are aligned with and provided in the anode buffer chamber 14, while the cathode electrodes 6 are aligned with and provided in the cathode buffer chamber 16. For instance, both the lid 2 and the unit 10 may each have alignment indicators (e.g., color coding, symbols, numbers, letters, etc.) to ensure that the lid 2 is correctly attached to unit 10. The seal 3 of the lid helps to ensure a secure tight fit between the lid 2 and unit 10 once assembled together. The electrodes 4, 6 are electrically connected to corresponding connectors 7 that reside on the outside of the lid 2, which in turn, are connected to a power source (not shown) via power cables 85. These connectors and cables may also be color coded to ensure correct connection to the appropriate positive or negative terminal of the power supply.
An electrical charge is then applied to the gel electrophoresis assembly 100 by turning on the power supply, whereby an effective electrical contact is established between the buffer solution in one chamber and the buffer solution in the other chamber through the gel matrices 22 (if present), 20, 24. For instance, referring to FIG. 6, the current flow may flow from the cathode buffer chamber 16 through the gel matrices 22, 20, 24 and into the anode buffer chamber 14. The present electrophoresis assembly 100 is run at an appropriate speed, temperature, power, current, voltage and duration depending upon the gels used and the target samples sufficient to separate the charged molecules into bands within the gel matrix 20.
Once separation has been completed, the power supply is turned off, the lid 2 removed from the monolithic gel electrophoresis unit 10 and the buffer 50 within the unit is poured off or out of the unit. Referring to FIGS. 1A, 5 and 7, the monolithic gel electrophoresis unit 10 has a disassembly mechanism 90 on each of the opposing sidewalls 13 for opening the unit 10 by separating the buffer chambers 14, 16 from one another and removing the processed gel matrix 20 for subsequent analytical procedures. This disassembly mechanism 90 preferably extends down the entire length of both sidewalls 13 for easy and efficient opening and disassembly of the unit 10. The disassembly mechanism 90 is preferably designed so that it causes no significant damage to the processed gel matrix 20 upon opening the unit 10. This disassembly region 90 may include, but is not limited to, a sealed groove or channel, a beveled portion, an adhesive seal, ultrasonic welding, a sealant, alumina foil, or any other mechanism for opening the gel electrophoresis unit 10 to remove the gel matrix 20, without damaging such gel matrix 20.
The monolithic gel electrophoresis unit 10 is preferably disposable, whereby after disassembling the unit 10 and removing the gel matrix 20 the buffer chambers 14, 16 are discarded and the lid 2, along with all its components, is retained for subsequent reuse. In this manner, one needs only to select a new monolithic gel electrophoresis unit 10, remove its seals, load target samples, provide the lid 2 over this new unit 10 and perform another electrophoresis run. However, as an alternate embodiment, the buffer chambers 14, 16 may be retained and reused by providing or fabricating a gel using known methods, followed by securing and sealing the gel between the buffer chambers 14, 16 for a subsequent electrophoresis run.
It should be appreciated that the present monolithic gel electrophoresis units 10 may have any desired dimensions as may be desired or required for further downstream processing of the gel matrix. For instance, the pre-fabricated, monolithic gel electrophoresis units 10 may be 9 cm×14 cm×3 cm (i.e., height×length (of outer walls 12)×width (thickness of bottom surface 11)), with a gel matrix 20 having a thickness ranging from <1 mm to about 3 mm, or greater, and requiring less than about 200 ml of buffer 50 within the entire unit 10. While not departing from the novel concepts of the invention, it should be appreciated that the present apparatus and systems may be designed so that the gel matrix is pre-cast horizontally, along with the buffer chambers and gel chambers residing horizontally, so that the unit 10 is processed in the horizontal position.
The present pre-fabricated, monolithic gel electrophoresis apparatus and systems are compact, all-in-one units that integrate the pre-cast gel matrix and preloaded buffer chambers into single, molded units that are structurally stable and avoid buffer leakage. The compact design of the present pre-fabricated, monolithic gel electrophoresis units 10 provides for easy transportation, storage and use of such units 10. With the pre-cast gel and the buffer both being preloaded into the pre-fabricated, monolithic gel electrophoresis units 10, no other component (other than the lid 2) is needed to run the gel. As such, a user merely needs to peel the seals off the unit, (perhaps reconstitute the buffer), load target samples into the wells, provide the lid 2 on top of the unit 10, and run the electrophoresis. Accordingly, the one or more embodiments of the invention provide easy and clean to use, low maintenance, easy clean-up (no washing of gel or buffer tanks/trays because the entire unit 10 is preferably discarded after use, however, the reusable lid 2 is retained for reuse) and cost effective pre-fabricated, monolithic gel electrophoresis apparatus and systems. There is also reduced upfront costs associated with the present apparatus and systems since one only needs to purchase the reusable lid 2, and not gel or buffer tanks/trays, clamps, gel holders, etc.
While the present invention has been particularly described, in conjunction with a specific preferred embodiment, it is evident that many alternatives, modifications and variations will be apparent to those skilled in the art in light of the foregoing description. It is therefore contemplated that the appended claims will embrace any such alternatives, modifications and variations as falling within the true scope and spirit of the present invention.

## Claims

1. A one-piece electrophoresis apparatus for use in performing gel electrophoresis comprising:
a first buffer chamber containing a buffer solution;
a second buffer chamber containing said buffer solution; and
at least one gel chamber containing a pre-cast gel comprising at least a separating gel matrix;
said gel chamber and said first and second buffer chambers are integrated with each other into a single unit that comprises a molded monolithic electrophoresis unit ready for use; and
a unit seal of said single unit residing on and completely covering and sealing top surfaces of the first buffer chamber, the second buffer chamber and the gel chamber containing the pre-cast gel prior to use of said single unit.

2. The apparatus of claim 1 wherein said first buffer chamber comprises an anode buffer chamber and said second buffer chamber comprises a cathode buffer chamber, said at least one gel chamber residing in electrical communication with both said cathode buffer chamber and said anode buffer chamber.

3. The apparatus of claim 2 wherein said gel chamber is in contact with said cathode buffer chamber and separated from said anode buffer chamber by a sealing gel of said pre-cast gel, said sealing gel filling an opening of said single unit and residing between and within said gel chamber and said anode buffer chamber.

4. The apparatus of claim 2 wherein said single unit further includes a third buffer chamber comprising another anode buffer chamber and a plurality of gel chambers each containing individual pre-cast gels, whereby each of said plurality of gel chambers is separated from said anode buffer chambers by a sealing gel of each of said pre-cast gels.

5. The apparatus of claim 1 wherein said pre-cast gel comprises in sequence a sealing gel, said separating gel matrix and a stacking gel.

6. The apparatus of claim 1 wherein said single unit further includes a buffer volume marker that indicates a fill line of said buffer.

7. The apparatus of claim 6 wherein said buffer solution comprises a preloaded buffer solution filled to said fill line.

8. The apparatus of claim 6 wherein said buffer solution comprises a concentrated buffer composition that requires reconstitution to said fill line prior to use of said single unit.

9. The apparatus of claim 1 further including inside said single unit a plurality of molded wells in contact with said pre-cast gel.

10. The apparatus of claim 1 wherein said unit seal comprises a removable layer selected from the group consisting of a removable film, tape, glued layer and adhesive layer.

11. The apparatus of claim 1 wherein said molded monolithic electrophoresis unit comprises a vertical molded monolithic electrophoresis unit.

12. The apparatus of claim 1 further including a gel chamber seal residing on a top surface of said gel chamber, thereby preventing contact between said pre-cast gel and said buffer solution prior to use of said single unit.

13. The apparatus of claim 12 wherein said gel chamber seal comprises a removable comb having a plurality of teeth extending into said pre-cast gel, thereby forming a plurality of wells in said pre-cast gel.

14. The apparatus of claim 12 wherein said gel chamber seal comprises a removable layer selected from the group consisting of a removable film, tape, glued layer and adhesive layer.

15. The apparatus of claim 1 further including well indicators residing inside said single unit for identifying a plurality of wells of said pre-cast separating gel matrix.

16. The apparatus of claim 15 wherein said well indicators reside on an interior wall of said single unit for transfer of said well indicators into or onto said pre-cast separating gel matrix.

17. The apparatus of claim 15 wherein said well indicators reside within or on said pre-cast separating gel matrix.

18. The apparatus of claim 1 further including a disassembly mechanism on at least a pair of exterior walls of said single unit that enable opening of said single unit after use thereof.

19. A system for use in performing gel electrophoresis comprising:
a reusable lid;
a pre-fabricated monolithic electrophoresis unit comprising:
an anode buffer chamber containing a buffer solution,
a cathode buffer chamber containing said buffer solution,
a gel chamber integrated with and residing between said anode and cathode buffer chambers and containing a pre-cast gel,
a unit seal residing on and completely covering and sealing top surfaces of the anode buffer chamber, the cathode buffer chamber and the gel chamber containing the pre-cast gel prior to use of the pre-fabricated unit, said pre-fabricated monolithic electrophoresis unit being ready for use in performing gel electrophoresis; and

an electrical connection between said reusable lid and said pre-fabricated monolithic electrophoresis unit for performing said gel electrophoresis.

20. The system of claim 19 wherein said lid has at least one anode electrode and at least one cathode electrode for enabling said electrical connection, said anode electrode residing within said buffer of said anode buffer chamber and said cathode electrode residing within said buffer of said cathode buffer chamber.

21. The system of claim 19 wherein said lid has a window or a temperature sensor at a top surface thereof.

22. A method for performing gel electrophoresis comprising:
providing a monolithic electrophoresis unit having a unit seal on a top surface thereof comprising:
an anode buffer chamber containing a buffer solution,
a cathode buffer chamber containing said buffer solution,
a gel chamber having a gel chamber seal integrated with and residing between said anode and cathode buffer chambers and containing a pre-cast gel, a unit seal residing on and completely covering top surfaces of the anode buffer chamber, the cathode buffer chamber and the gel chamber prior to use of the monolithic electrophoresis unit;

removing said unit seal and said gel chamber seal;
loading a target sample into said monolithic electrophoresis unit;
attaching a reusable lid to said top surface of said monolithic electrophoresis unit; and
performing electrophoresis to said pre-cast gel by providing an electrical connection through said reusable lid into said monolithic electrophoresis unit.

23. The method of claim 22 further including, once said electrophoresis performance is complete, opening said monolithic electrophoresis unit at a disassembly section thereof, followed by removing the processed pre-cast gel.

24. The method of claim 22 further including reconstituting said buffer solution to a buffer fill line of said monolithic electrophoresis unit.

25. The method of claim 22 wherein said target sample is loaded into a plurality of wells that have been pre-fabricated inside said monolithic electrophoresis unit.