Exhibit 31.1
 CERTIFICATION
  1.
    
    I have reviewed this report on Form 10-Q of Northfield Laboratories Inc. (the Registrant);2.
    
    Based on my knowledge, this report does not contain any untrue statement of a material fact
or omit to state a material fact necessary to make the statements made, in light of the
circumstances under which such statements were made, not misleading with respect to the
period covered by this report;3.
    
    Based on my knowledge, the financial statements, and other financial information included
in this report, fairly present in all material respects the financial condition, results of
operations and cash flows of the Registrant as of, and for, the periods presented in this
report;4.
    
    The Registrants other certifying officer and I are responsible for establishing and
maintaining disclosure controls and procedures (as defined in Exchange Act Rules 13a-15(e)
and 15d-15(e)) and internal control over financial reporting (as defined in Exchange Act
Rules 13a-15(f) and 15d-15(f)) for the Registrant and have:
 a.
    
    Designed such disclosure controls and procedures, or caused such
disclosure controls and procedures to be designed under our
supervision, to ensure that material information relating to the
Registrant, including its consolidated subsidiaries, is made known to
us by others within those entities, particularly during the period in
which this quarterly report is being prepared;b.
    
    Designed such internal control over financial reporting, or caused
such internal control over financial reporting to be designed under
our supervision, to provide reasonable assurance regarding the
reliability of financial reporting and the preparation of financial
statements for external purposes in accordance with generally accepted
accounting principles;c.
    
    Evaluated the effectiveness of the Registrants disclosure controls
and procedures and presented in this report our conclusions about the
effectiveness of the disclosure controls and procedures, as of the end
of the period covered by this report based on such evaluation; andd.
    
    Disclosed in this report any change in the Registrants internal
control over financial reporting that occurred during the Registrants
most recent fiscal quarter that has materially affected, or is
reasonably likely to materially affect, the Registrants internal
control over financial reporting; and
 5.
    
    The Registrants other certifying officer and I have disclosed, based
on our most recent evaluation of internal control over financial
reporting, to the Registrants auditors and the audit committee of
Registrants Board of Directors (or persons performing the equivalent
functions):
 a.
    
    all significant deficiencies and
material weaknesses in the design or
operation of internal control over
financial reporting which are
reasonably likely to adversely affect
the Registrants ability to record,
process, summarize and report financial
information; and
 b.
    
    any fraud, whether or not material,
that involves management or other
employees who have a significant role
in the Registrants internal control
over financial reporting.
 Date: April 9, 2009
    /s/ Steven A. GouldSteven A. Gould, M.D.Chief Executive Officer