# Patent

## Title
Piperazine carbamates and methods of making and using same

## Abstract

Provided herein are piperazine carbamates and pharmaceutical compositions comprising said compounds. The subject compounds and compositions are useful as modulators of MAGL and/or ABHD6. Furthermore, the subject compounds and compositions are useful for the treatment of pain.

## Background
CROSS-REFERENCE
This application claims benefit of U.S. Provisional Application No. 62/135,072, filed on Mar. 18, 2015, which is herein incorporated by reference in its entirety.


BACKGROUND
Monoacylglycerol lipase (MAGL) is an enzyme responsible for hydrolyzing endocannabinoids such as 2-AG (2-arachidonoylglycerol), an arachidonate based lipid, in the nervous system. The serine hydrolase α-β-hydrolase domain 6 (ABHD6) is another lipid mediator.
BRIEF SUMMARY OF THE INVENTION
This disclosure provides, for example, compounds and compositions which are modulators of MAGL and/or ABHD6, and their use as medicinal agents, processes for their preparation, and pharmaceutical compositions that include disclosed compounds as at least one active ingredient. The disclosure also provides for the use of disclosed compounds as medicaments and/or in the manufacture of medicaments for the inhibition of MAGL and/or ABHD6 activity in warm-blooded animals such as humans.
One embodiment provides a compound of Formula (I):




wherein:

    R1 is halogen, —OR3, —CN, aryl (optionally substituted by one, two, or three moieties each independently selected from R4), aryloxy (optionally substituted by one, two, or three moieties each independently selected from R4), heteroaryl (optionally substituted by one, two, or three moieties each independently selected from R4), heteroaryloxy (optionally substituted by one, two, or three moieties each independently selected from R4), heterocycle (optionally substituted by one, two, or three moieties each independently selected from R4), cycloalkyl (optionally substituted by one, two, or three moieties each independently selected from R4), optionally substituted C1-6 alkyl, optionally substituted C2-6 alkenyl, optionally substituted C2-6 alkynyl, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, —NR8C(O)NR9R10, —C(O)R9, —C(O)OR9, —C(O)NR9R10, —S(O)wR11, or —SH;
    R2 is —NR5R6, -alkyl(NR14R15), or —OR7;
    each R3 is independently selected from H, C1-6 alkyl, haloalkyl, and aminoalkyl;
    each R4 is independently selected from halogen, —OR3, —CN, nitro, optionally substituted C1-6 alkyl, optionally substituted C1-6 alkenyl, optionally substituted C1-6 alkynyl, cycloalkyl, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, —NR8C(O)NR9R10, —C(O)R9, —C(O)OR9, —C(O)NR9R10, —S(O)wR11, and —SH;
    R5 and R6, together with the nitrogen to which they are attached, form
    
        (i) a monocyclic heterocycle, a fused bicyclic heterocycle, or a spirocycle; or
        (ii) a 7-8 membered bridged heterocyclic ring optionally containing an additional O, N, or S;
        wherein the monocyclic heterocycle, the fused bicyclic heterocycle, or the spirocycle is substituted with one or more substituents independently selected from optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocycle, optionally substituted cycloalkyl, haloalkyl, —OR7, —NR12R13, —C(O)OR9, —C(O)NR9R10, —NR8SO2R9, —NR8C(O)OR9, and —NR8C(O)NR9R10; and the monocyclic heterocycle, the fused bicyclic heterocycle, or the spirocycle optionally contains an additional O, N, or S; and
        the 7-8 membered bridged heterocyclic ring is optionally substituted with one or more substituents independently selected from halogen, —CN, oxo, optionally substituted C1-6 alkyl, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocycle, optionally substituted cycloalkyl, —S(O)wR11, —OR3, —OR7, —C(O)R9, —C(O)OR9, —C(O)NR9R10, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, and —NR8C(O)NR9R10;
    
    
    R7 is alkynyl or (alkynyl)alkyl;
    each R8 is independently selected from H, optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl;
    each R9 and R10 is independently selected from H, optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl; or R9 and R10 together with the nitrogen to which they are attached form an optionally substituted heterocycle optionally containing an additional O, N, or S;
    each R11 is independently selected from optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl;
    R12 and R13 together with the nitrogen to which they are attached form an optionally substituted heterocycle optionally containing an additional O, N, or S;
    R14 and R15 together with the nitrogen to which they are attached form an optionally substituted heterocycle optionally containing an additional O, N, or S; and
    w is 0, 1, or 2;

or a solvate, hydrate, tautomer, N-oxide, or pharmaceutically acceptable salt thereof.



Another embodiment provides a compound of Formula (Ia):




wherein:

    R1 is halogen, —OR3, —CN, aryl (optionally substituted by one, two, or three moieties each independently selected from R4), aryloxy (optionally substituted by one, two, or three moieties each independently selected from R4), heteroaryl (optionally substituted by one, two, or three moieties each independently selected from R4), heteroaryloxy (optionally substituted by one, two, or three moieties each independently selected from R4), heterocycle (optionally substituted by one, two, or three moieties each independently selected from R4), cycloalkyl (optionally substituted by one, two, or three moieties each independently selected from R4), optionally substituted C1-6 alkyl, optionally substituted C2-6 alkenyl, optionally substituted C2-6 alkynyl, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, —NR8C(O)NR9R10, —C(O)R9, —C(O)OR9, —C(O)NR9R10, —S(O)wR11, or —SH;
    R2 is —C(O)C(O)OR7;
    each R3 is independently selected from H, C1-6 alkyl, haloalkyl, and aminoalkyl;
    each R4 is independently selected from halogen, —OR3, —CN, nitro, optionally substituted C1-6 alkyl, optionally substituted C1-6 alkenyl, optionally substituted C1-6 alkynyl, cycloalkyl, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, —NR8C(O)NR9R10, —C(O)R9, —C(O)OR9, —C(O)NR9R10, —S(O)wR11, and —SH;
    R7 is H or C1-6 alkyl;
    each R8 is independently selected from H, optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl;
    each R9 and R10 is independently selected from H, optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl; or R9 and R10 together with the nitrogen to which they are attached form an optionally substituted heterocycle optionally containing an additional O, N, or S;
    each R11 is independently selected from optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl; and
    w is 0, 1, or 2;

or a solvate, hydrate, tautomer, N-oxide, or pharmaceutically acceptable salt thereof.



Another embodiment provides a compound of Formula (II):




wherein:

    R1 is halogen, —OR3, —CN, aryl (optionally substituted by one, two, or three moieties each independently selected from R4), aryloxy (optionally substituted by one, two, or three moieties each independently selected from R4), heteroaryl (optionally substituted by one, two, or three moieties each independently selected from R4), heteroaryloxy (optionally substituted by one, two, or three moieties each independently selected from R4), heterocycle (optionally substituted by one, two, or three moieties each independently selected from R4), cycloalkyl (optionally substituted by one, two, or three moieties each independently selected from R4), optionally substituted C1-6 alkyl, optionally substituted C2-6 alkenyl, optionally substituted C2-6 alkynyl, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, —NR8C(O)NR9R10, —C(O)R9, —C(O)OR9, —C(O)NR9R10, —S(O)wR11, or —SH;
    each R3 is independently selected from H, C1-6 alkyl, haloalkyl, and aminoalkyl;
    each R4 is independently selected from halogen, —OR3, —CN, nitro, optionally substituted C1-6 alkyl, optionally substituted C1-6 alkenyl, optionally substituted C1-6 alkynyl, cycloalkyl, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, —NR8C(O)NR9R10, —C(O)R9, —C(O)OR9, —C(O)NR9R10, —S(O)wR11, and —SH;
    R5 and R6, together with the nitrogen to which they are attached, form
    
        (i) a monocyclic heterocycle, a fused bicyclic heterocycle, or a spirocycle; or
        (ii) a 7-8 membered bridged heterocyclic ring optionally containing an additional O, N, or S;
        wherein the monocyclic heterocycle, the fused bicyclic heterocycle, or the spirocycle is substituted with one or more substituents independently selected from optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocycle, optionally substituted cycloalkyl, haloalkyl, —OR7, —NR12R13, —C(O)OR9, —C(O)NR9R10, —NR8SO2R9, —NR8C(O)OR9, and —NR8C(O)NR9R10; and the monocyclic heterocycle, the fused bicyclic heterocycle, or the spirocycle optionally contains an additional O, N, or S; and
        the 7-8 membered bridged heterocyclic ring is optionally substituted with one or more substituents independently selected from halogen, —CN, oxo, optionally substituted C1-6 alkyl, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocycle, optionally substituted cycloalkyl, —S(O)wR11, —OR3, —OR7, —C(O)R9, —C(O)OR9, —C(O)NR9R10, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, and —NR8C(O)NR9R10;
    
    
    R7 is alkynyl or (alkynyl)alkyl;
    each R8 is independently selected from H, optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl;
    each R9 and R10 is independently selected from H, optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl; or R9 and R10 together with the nitrogen to which they are attached form an optionally substituted heterocycle optionally containing an additional O, N, or S;
    each R11 is independently selected from optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl;
    R12 and R13 together with the nitrogen to which they are attached form an optionally substituted heterocycle optionally containing an additional O, N, or S; and
    w is 0, 1, or 2;

or a solvate, hydrate, tautomer, N-oxide, or pharmaceutically acceptable salt thereof.



Another embodiment provides a compound of Formula (IIa):




wherein:

    R1 is halogen, —OR3, —CN, aryl (optionally substituted by one, two, or three moieties each independently selected from R4), aryloxy (optionally substituted by one, two, or three moieties each independently selected from R4), heteroaryl (optionally substituted by one, two, or three moieties each independently selected from R4), heteroaryloxy (optionally substituted by one, two, or three moieties each independently selected from R4), heterocycle (optionally substituted by one, two, or three moieties each independently selected from R4), cycloalkyl (optionally substituted by one, two, or three moieties each independently selected from R4), optionally substituted C1-6 alkyl, optionally substituted C2-6 alkenyl, optionally substituted C2-6 alkynyl, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, —NR8C(O)NR9R10, —C(O)R9, —C(O)OR9, —C(O)NR9R10, —S(O)wR11, or —SH;
    each R3 is independently selected from H, C1-6 alkyl, haloalkyl, and aminoalkyl;
    each R4 is independently selected from halogen, —OR3, —CN, nitro, optionally substituted C1-6 alkyl, optionally substituted C1-6 alkenyl, optionally substituted C1-6 alkynyl, cycloalkyl, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, —NR8C(O)NR9R10, —C(O)R9, —C(O)OR9, —C(O)NR9R10, —S(O)wR11, and —SH;
    R5 and R6, together with the nitrogen to which they are attached, form a monocyclic heterocycle, a fused bicyclic heterocycle, or a spirocycle; wherein the monocyclic heterocycle, the fused bicyclic heterocycle, or the spirocycle is substituted with hydroxyalkyl; and the monocyclic heterocycle, the fused bicyclic heterocycle, or the spirocycle optionally contains an additional O, N, or S;
    each R8 is independently selected from H, optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl;
    each R9 and R10 is independently selected from H, optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl; or R9 and R10 together with the nitrogen to which they are attached form an optionally substituted heterocycle optionally containing an additional O, N, or S;
    each R11 is independently selected from optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl; and
    w is 0, 1, or 2;

or a solvate, hydrate, tautomer, N-oxide, or pharmaceutically acceptable salt thereof.



Another embodiment provides a compound of Formula (III):




wherein:

    R1 is halogen, —OR3, —CN, aryl (optionally substituted by one, two, or three moieties each independently selected from R4), aryloxy (optionally substituted by one, two, or three moieties each independently selected from R4), heteroaryl (optionally substituted by one, two, or three moieties each independently selected from R4), heteroaryloxy (optionally substituted by one, two, or three moieties each independently selected from R4), heterocycle (optionally substituted by one, two, or three moieties each independently selected from R4), cycloalkyl (optionally substituted by one, two, or three moieties each independently selected from R4), optionally substituted C1-6 alkyl, optionally substituted C2-6 alkenyl, optionally substituted C2-6 alkynyl, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, —NR8C(O)NR9R10, —C(O)R9, —C(O)OR9, —C(O)NR9R10, —S(O)wR11, or —SH;
    each R3 is independently selected from H, C1-6 alkyl, haloalkyl, and aminoalkyl;
    each R4 is independently selected from halogen, —OR3, —CN, nitro, optionally substituted C1-6 alkyl, optionally substituted C1-6 alkenyl, optionally substituted C1-6 alkynyl, cycloalkyl, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, —NR8C(O)NR9R10, —C(O)R9, —C(O)OR9, —C(O)NR9R10, —S(O)wR11, and —SH;
    each R8 is independently selected from H, optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl;
    each R9 and R10 is independently selected from H, optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl; or R9 and R10 together with the nitrogen to which they are attached form an optionally substituted heterocycle optionally containing an additional O, N, or S;
    each R11 is independently selected from optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl;
    R14 and R15 together with the nitrogen to which they are attached form an optionally substituted heterocycle optionally containing an additional O, N, or S;
    w is 0, 1, or 2; and
    x is 1, 2, or 3;

or a solvate, hydrate, tautomer, N-oxide, stereoisomer, or pharmaceutically acceptable salt thereof.



Another embodiment provides a compound of Formula (IV):




wherein:

    A and B are independently a bond or CR18R19;
    C is O or CR18R19OCR20R21;
    D is CR18R19 or CR18R19CR20R21;
    R1 is halogen, —OR3, —CN, aryl (optionally substituted by one, two, or three moieties each independently selected from R4), aryloxy (optionally substituted by one, two, or three moieties each independently selected from R4), heteroaryl (optionally substituted by one, two, or three moieties each independently selected from R4), heteroaryloxy (optionally substituted by one, two, or three moieties each independently selected from R4), heterocycle (optionally substituted by one, two, or three moieties each independently selected from R4), cycloalkyl (optionally substituted by one, two, or three moieties each independently selected from R4), optionally substituted C1-6 alkyl, optionally substituted C2-6 alkenyl, optionally substituted C2-6 alkynyl, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, —NR8C(O)NR9R10, —C(O)R9, —C(O)OR9, —C(O)NR9R10, —S(O)wR11, or —SH;
    each R3 is independently selected from H, C1-6 alkyl, haloalkyl, and aminoalkyl;
    each R4 is independently selected from halogen, —OR3, —CN, nitro, optionally substituted C1-6 alkyl, optionally substituted C1-6 alkenyl, optionally substituted C1-6 alkynyl, cycloalkyl, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, —NR8C(O)NR9R10, —C(O)R9, —C(O)OR9, —C(O)NR9R10, —S(O)wR11, and —SH;
    each R8 is independently selected from H, optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl;
    each R9 and R10 is independently selected from H, optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl; or R9 and R10 together with the nitrogen to which they are attached form an optionally substituted heterocycle optionally containing an additional O, N, or S;
    each R11 is independently selected from optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl;
    R17 is H, optionally substituted C1-6 alkyl, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocycle, optionally substituted cycloalkyl, —S(O)wR11, —C(O)R9, —C(O)OR9, or —C(O)NR9R10;
    each R18, R19, R20, and R21 is independently selected from H, optionally substituted C1-6 alkyl, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocycle, optionally substituted cycloalkyl, —C(O)R9, —C(O)OR9, and —C(O)NR9R10; and
    w is 0, 1, or 2;

or a solvate, hydrate, tautomer, N-oxide, stereoisomer, or pharmaceutically acceptable salt thereof.



Another embodiment provides a pharmaceutical composition comprising a piperazine carbamate described herein, or a solvate, hydrate, tautomer, N-oxide, stereoisomer, or a pharmaceutically acceptable salt thereof, and at least one pharmaceutically acceptable excipient.
Another embodiment provides a method of treating pain in a patient, comprising administering a therapeutically effective amount of a piperazine carbamate described herein to a patient in need thereof to treat said pain. In some embodiments, the pain is neuropathic pain.


DETAILED DESCRIPTION OF THE INVENTION
This disclosure is directed, at least in part, to MAGL and/or ABHD6 modulators or inhibitors. For example, provided herein are compounds capable of inhibiting MAGL and/or ABHD6.
As used herein and in the appended claims, the singular forms “a,” “and,” and “the” include plural referents unless the context clearly dictates otherwise. Thus, for example, reference to “an agent” includes a plurality of such agents, and reference to “the cell” includes reference to one or more cells (or to a plurality of cells) and equivalents thereof. When ranges are used herein for physical properties, such as molecular weight, or chemical properties, such as chemical formulae, all combinations and subcombinations of ranges and specific embodiments therein are intended to be included. The term “about” when referring to a number or a numerical range means that the number or numerical range referred to is an approximation within experimental variability (or within statistical experimental error), and thus the number or numerical range varies between 1% and 15% of the stated number or numerical range. The term “comprising” (and related terms such as “comprise” or “comprises” or “having” or “including”) is not intended to exclude that which in other certain embodiments, for example, an embodiment of any composition of matter, composition, method, or process, or the like, described herein, may “consist of” or “consist essentially of” the described features.
Definitions
As used in the specification and appended claims, unless specified to the contrary, the following terms have the meaning indicated below.
“Amino” refers to the —NH2 radical.
“Cyano” refers to the —CN radical.
“Nitro” refers to the —NO2 radical.
“Oxa” refers to the —O— radical.
“Oxo” refers to the ═O radical.
“Thioxo” refers to the ═S radical.
“Imino” refers to the ═N—H radical.
“Oximo” refers to the ═N—OH radical.
“Alkyl” refers to a straight or branched hydrocarbon chain radical consisting solely of carbon and hydrogen atoms, containing no unsaturation, having from one to fifteen carbon atoms (e.g., C1-C15 alkyl). In certain embodiments, an alkyl comprises one to thirteen carbon atoms (e.g., C1-C13 alkyl). In certain embodiments, an alkyl comprises one to eight carbon atoms (e.g., C1-C8 alkyl). In other embodiments, an alkyl comprises one to five carbon atoms (e.g., C1-C5 alkyl). In other embodiments, an alkyl comprises one to four carbon atoms (e.g., C1-C4 alkyl). In other embodiments, an alkyl comprises one to three carbon atoms (e.g., C1-C3 alkyl). In other embodiments, an alkyl comprises one to two carbon atoms (e.g., C1-C2 alkyl). In other embodiments, an alkyl comprises one carbon atom (e.g., C1 alkyl). In other embodiments, an alkyl comprises five to fifteen carbon atoms (e.g., C5-C15 alkyl). In other embodiments, an alkyl comprises five to eight carbon atoms (e.g., C5-C8 alkyl). In other embodiments, an alkyl comprises two to five carbon atoms (e.g., C2-C5 alkyl). In other embodiments, an alkyl comprises three to five carbon atoms (e.g., C3-C5 alkyl). In other embodiments, the alkyl group is selected from methyl, ethyl, 1-propyl (n-propyl), 1-methylethyl (iso-propyl), 1-butyl (n-butyl), 1-methylpropyl (sec-butyl), 2-methylpropyl (iso-butyl), 1,1-dimethylethyl (tert-butyl), 1-pentyl (n-pentyl). The alkyl is attached to the rest of the molecule by a single bond. Unless stated otherwise specifically in the specification, an alkyl group is optionally substituted by one or more of the following substituents: halo, cyano, nitro, oxo, thioxo, imino, oximo, trimethylsilanyl, —ORa, —SRa, —OC(O)—Ra, —N(Ra)2, —C(O)Ra, —C(O)ORa, —C(O)N(Ra)2, —N(Ra)C(O)ORf, —OC(O)—NRaRf, —N (Ra)C(O)Rf, —N(Ra)S(O)tRf (where t is 1 or 2), —S(O)tORa (where t is 1 or 2), —S(O)tRf (where t is 1 or 2) and —S(O)tN(Ra)2 (where t is 1 or 2) where each Ra is independently hydrogen, alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocyclyl, heterocyclylalkyl, heteroaryl or heteroarylalkyl, and each Rf is independently alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocyclyl, heterocyclylalkyl, heteroaryl or heteroarylalkyl.
“Alkoxy” refers to a radical bonded through an oxygen atom of the formula —O-alkyl, where alkyl is an alkyl chain as defined above.
“Alkenyl” refers to a straight or branched hydrocarbon chain radical group consisting solely of carbon and hydrogen atoms, containing at least one carbon-carbon double bond, and having from two to twelve carbon atoms. In certain embodiments, an alkenyl comprises two to eight carbon atoms. In certain embodiments, an alkenyl comprises two to six carbon atoms. In other embodiments, an alkenyl comprises two to four carbon atoms. The alkenyl is attached to the rest of the molecule by a single bond, for example, ethenyl (i.e., vinyl), prop-1-enyl (i.e., allyl), but-1-enyl, pent-1-enyl, penta-1,4-dienyl, and the like. Unless stated otherwise specifically in the specification, an alkenyl group is optionally substituted by one or more of the following substituents: halo, cyano, nitro, oxo, thioxo, imino, oximo, trimethylsilanyl, —ORa, —SRa, —OC(O)—Ra, —N(Ra)2, —C(O)Ra, —C(O)ORa, —C(O)N(Ra)2, —N(Ra)C(O)ORf, —OC(O)—NRaRf, —N(Ra)C(O)Rf, —N(Ra)S(O)tRf (where t is 1 or 2), —S(O)tORa (where t is 1 or 2), —S(O)tRf (where t is 1 or 2) and —S(O)tN(Ra)2 (where t is 1 or 2) where each Ra is independently hydrogen, alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocyclyl, heterocyclylalkyl, heteroaryl or heteroarylalkyl, and each Rf is independently alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocyclyl, heterocyclylalkyl, heteroaryl or heteroarylalkyl.
“Alkynyl” refers to a straight or branched hydrocarbon chain radical group consisting solely of carbon and hydrogen atoms, containing at least one carbon-carbon triple bond, having from two to twelve carbon atoms. In certain embodiments, an alkynyl comprises two to eight carbon atoms. In certain embodiments, an alkynyl comprises two to six carbon atoms. In other embodiments, an alkynyl has two to four carbon atoms. The alkynyl is attached to the rest of the molecule by a single bond, for example, ethynyl, propynyl, butynyl, pentynyl, hexynyl, and the like. Unless stated otherwise specifically in the specification, an alkynyl group is optionally substituted by one or more of the following substituents: halo, cyano, nitro, oxo, thioxo, imino, oximo, trimethylsilanyl, —ORa, —SRa, —OC(O)—Ra, —N(Ra)2, —C(O)Ra, —C(O)ORa, —C(O)N(Ra)2, —N(Ra)C(O)ORf, —OC(O)—NRaRf, —N (Ra)C(O)Rf, —N(Ra)S(O)tRf (where t is 1 or 2), —S(O)tORa (where t is 1 or 2), —S(O)tRf (where t is 1 or 2) and —S(O)tN(Ra)2 (where t is 1 or 2) where each Ra is independently hydrogen, alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocyclyl, heterocyclylalkyl, heteroaryl or heteroarylalkyl, and each Rf is independently alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocyclyl, heterocyclylalkyl, heteroaryl or heteroarylalkyl.
“Alkylene” or “alkylene chain” refers to a straight or branched divalent hydrocarbon chain linking the rest of the molecule to a radical group, consisting solely of carbon and hydrogen, containing no unsaturation and having from one to twelve carbon atoms, for example, methylene, ethylene, propylene, n-butylene, and the like. The alkylene chain is attached to the rest of the molecule through a single bond and to the radical group through a single bond. The points of attachment of the alkylene chain to the rest of the molecule and to the radical group are through one carbon in the alkylene chain or through any two carbons within the chain. In certain embodiments, an alkylene comprises one to eight carbon atoms (e.g., C1-C8 alkylene). In other embodiments, an alkylene comprises one to five carbon atoms (e.g., C1-C5 alkylene). In other embodiments, an alkylene comprises one to four carbon atoms (e.g., C1-C4 alkylene). In other embodiments, an alkylene comprises one to three carbon atoms (e.g., C1-C3 alkylene). In other embodiments, an alkylene comprises one to two carbon atoms (e.g., C1-C2 alkylene). In other embodiments, an alkylene comprises one carbon atom (e.g., C1 alkylene). In other embodiments, an alkylene comprises five to eight carbon atoms (e.g., C5-C8 alkylene). In other embodiments, an alkylene comprises two to five carbon atoms (e.g., C2-C5 alkylene). In other embodiments, an alkylene comprises three to five carbon atoms (e.g., C3-C5 alkylene). Unless stated otherwise specifically in the specification, an alkylene chain is optionally substituted by one or more of the following substituents: halo, cyano, nitro, oxo, thioxo, imino, oximo, trimethylsilanyl, —ORa, —SRa, —OC(O)—Ra, —N(Ra)2, —C(O)Ra, —C(O)ORa, —C(O)N(Ra)2, —N(Ra)C(O)ORf, —OC(O)—NRaRf, —N (Ra)C(O)Rf, —N(Ra)S(O)tRf (where t is 1 or 2), —S(O)tORa (where t is 1 or 2), —S(O)tRf (where t is 1 or 2) and —S(O)tN(Ra)2 (where t is 1 or 2) where each Ra is independently hydrogen, alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocyclyl, heterocyclylalkyl, heteroaryl or heteroarylalkyl, and each Rf is independently alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocyclyl, heterocyclylalkyl, heteroaryl or heteroarylalkyl.
“Aryl” refers to a radical derived from an aromatic monocyclic or multicyclic hydrocarbon ring system by removing a hydrogen atom from a ring carbon atom. The aromatic monocyclic or multicyclic hydrocarbon ring system contains only hydrogen and carbon from five to eighteen carbon atoms, where at least one of the rings in the ring system is fully unsaturated, i.e., it contains a cyclic, delocalized (4n+2) π-electron system in accordance with the Hückel theory. The ring system from which aryl groups are derived include, but are not limited to, groups such as benzene, fluorene, indane, indene, tetralin and naphthalene. Unless stated otherwise specifically in the specification, the term “aryl” or the prefix “ar-” (such as in “aralkyl”) is meant to include aryl radicals optionally substituted by one or more substituents independently selected from alkyl, alkenyl, alkynyl, halo, fluoroalkyl, cyano, nitro, optionally substituted aryl, optionally substituted aralkyl, optionally substituted aralkenyl, optionally substituted aralkynyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, optionally substituted heteroaryl, optionally substituted heteroarylalkyl, —Rb—ORa, —Rb—OC(O)—Ra, —Rb—OC(O)—ORa, —Rb—OC(O)—N(Ra)2, —Rb—N(Ra)2, —Rb—C(O)Ra, —Rb—C(O)ORa, —Rb—C(O)N(Ra)2, —Rb—O—Rc—C(O)N(Ra)2, —Rb—N(Ra)C(O)ORa, —Rb—N(Ra)C(O) Ra, —Rb—N(Ra)S(O)tRa (where t is 1 or 2), —Rb—S(O)tORa (where t is 1 or 2), —Rb—S(O)tRa (where t is 1 or 2) and —Rb—S(O)tN(Ra)2 (where t is 1 or 2), where each Ra is independently hydrogen, alkyl, fluoroalkyl, cycloalkyl, cycloalkylalkyl, aryl (optionally substituted with one or more halo groups), aralkyl, heterocyclyl, heterocyclylalkyl, heteroaryl or heteroarylalkyl, each Rb is independently a direct bond or a straight or branched alkylene or alkenylene chain, and Rc is a straight or branched alkylene or alkenylene chain, and where each of the above substituents is unsubstituted unless otherwise indicated.
“Aryloxy” refers to a radical bonded through an oxygen atom of the formula —O-aryl, where aryl is as defined above.
“Aralkyl” refers to a radical of the formula —Rc-aryl where Rc is an alkylene chain as defined above, for example, methylene, ethylene, and the like. The alkylene chain part of the aralkyl radical is optionally substituted as described above for an alkylene chain. The aryl part of the aralkyl radical is optionally substituted as described above for an aryl group.
“Aralkenyl” refers to a radical of the formula —Rd-aryl where Rd is an alkenylene chain as defined above. The aryl part of the aralkenyl radical is optionally substituted as described above for an aryl group. The alkenylene chain part of the aralkenyl radical is optionally substituted as defined above for an alkenylene group.
“Aralkynyl” refers to a radical of the formula —Re-aryl, where Re is an alkynylene chain as defined above. The aryl part of the aralkynyl radical is optionally substituted as described above for an aryl group. The alkynylene chain part of the aralkynyl radical is optionally substituted as defined above for an alkynylene chain.
“Carbocyclyl” refers to a stable non-aromatic monocyclic or polycyclic hydrocarbon radical consisting solely of carbon and hydrogen atoms, which includes fused or bridged ring systems, having from three to fifteen carbon atoms. In certain embodiments, a carbocyclyl comprises three to ten carbon atoms. In other embodiments, a carbocyclyl comprises five to seven carbon atoms. The carbocyclyl is attached to the rest of the molecule by a single bond.
Carbocyclyl is saturated, (i.e., containing single C—C bonds only) or unsaturated (i.e., containing one or more double bonds or triple bonds). A fully saturated carbocyclyl radical is also referred to as “cycloalkyl.” Examples of monocyclic cycloalkyls include, e.g., cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, and cyclooctyl. In certain embodiments, a cycloalkyl comprises three to eight carbon atoms (e.g., C3-C8 cycloalkyl). In other embodiments, a cycloalkyl comprises three to seven carbon atoms (e.g., C3-C7 cycloalkyl). In other embodiments, a cycloalkyl comprises three to six carbon atoms (e.g., C3-C6 cycloalkyl). In other embodiments, a cycloalkyl comprises three to five carbon atoms (e.g., C3-C5 cycloalkyl). In other embodiments, a cycloalkyl comprises three to four carbon atoms (e.g., C3-C4 cycloalkyl). An unsaturated carbocyclyl is also referred to as “cycloalkenyl.” Examples of monocyclic cycloalkenyls include, e.g., cyclopentenyl, cyclohexenyl, cycloheptenyl, and cyclooctenyl. Polycyclic carbocyclyl radicals include, for example, adamantyl, norbornyl (i.e., bicyclo[2.2.1]heptanyl), norbornenyl, decalinyl, 7,7-dimethyl-bicyclo[2.2.1]heptanyl, and the like. Unless otherwise stated specifically in the specification, the term “carbocyclyl” is meant to include carbocyclyl radicals that are optionally substituted by one or more substituents independently selected from alkyl, alkenyl, alkynyl, halo, fluoroalkyl, oxo, thioxo, cyano, nitro, optionally substituted aryl, optionally substituted aralkyl, optionally substituted aralkenyl, optionally substituted aralkynyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, optionally substituted heteroaryl, optionally substituted heteroarylalkyl, —Rb—ORa, —Rb—OC(O)—Ra, —Rb—OC(O)—ORa, —Rb—OC(O)—N(Ra)2, —Rb—N(Ra)2, —Rb—C(O)Ra, —Rb—C(O)ORa, —Rb—C(O)N(Ra)2, —Rb—O—Rc—C(O)N(Ra)2, —Rb—N(Ra)C(O)ORa, —Rb—N(Ra)C(O) Ra, —Rb—N(Ra)S(O)tRa (where t is 1 or 2), —Rb—S(O)tORa (where t is 1 or 2), —Rb—S(O)tRa (where t is 1 or 2) and —Rb—S(O)tN(Ra)2 (where t is 1 or 2), where each Ra is independently hydrogen, alkyl, fluoroalkyl, cycloalkyl, cycloalkylalkyl, aryl, aralkyl, heterocyclyl, heterocyclylalkyl, heteroaryl or heteroarylalkyl, each Rb is independently a direct bond or a straight or branched alkylene or alkenylene chain, and Rc is a straight or branched alkylene or alkenylene chain, and where each of the above substituents is unsubstituted unless otherwise indicated.
“Carbocyclylalkyl” refers to a radical of the formula —Rc-carbocyclyl where Rc is an alkylene chain as defined above. The alkylene chain and the carbocyclyl radical is optionally substituted as defined above.
“Halo” or “halogen” refers to bromo, chloro, fluoro or iodo substituents.
“Fluoroalkyl” refers to an alkyl radical, as defined above, that is substituted by one or more fluoro radicals, as defined above, for example, trifluoromethyl, difluoromethyl, fluoromethyl, 2,2,2-trifluoroethyl, 1-fluoromethyl-2-fluoroethyl, and the like. In some embodiments, the alkyl part of the fluoroalkyl radical is optionally substituted as defined above for an alkyl group.
“Heterocyclyl” refers to a stable 3- to 18-membered non-aromatic ring radical that comprises two to twelve carbon atoms and from one to six heteroatoms selected from nitrogen, oxygen and sulfur. Unless stated otherwise specifically in the specification, the heterocyclyl radical is a monocyclic, bicyclic, tricyclic or tetracyclic ring system, which includes fused or bridged ring systems. The heteroatoms in the heterocyclyl radical are optionally oxidized. One or more nitrogen atoms, if present, are optionally quaternized. The heterocyclyl radical is partially or fully saturated. In some embodiments, the heterocyclyl is attached to the rest of the molecule through any atom of the ring(s). Examples of such heterocyclyl radicals include, but are not limited to, dioxolanyl, thienyl[1,3]dithianyl, decahydroisoquinolyl, imidazolinyl, imidazolidinyl, isothiazolidinyl, isoxazolidinyl, morpholinyl, octahydroindolyl, octahydroisoindolyl, 2-oxopiperazinyl, 2-oxopiperidinyl, 2-oxopyrrolidinyl, oxazolidinyl, piperidinyl, piperazinyl, 4-piperidonyl, pyrrolidinyl, pyrazolidinyl, quinuclidinyl, thiazolidinyl, tetrahydrofuryl, trithianyl, tetrahydropyranyl, thiomorpholinyl, thiamorpholinyl, 1-oxo-thiomorpholinyl, and 1,1-dioxo-thiomorpholinyl. Unless stated otherwise specifically in the specification, the term “heterocyclyl” is meant to include heterocyclyl radicals as defined above that are optionally substituted by one or more substituents selected from alkyl, alkenyl, alkynyl, halo, fluoroalkyl, oxo, thioxo, cyano, nitro, optionally substituted aryl, optionally substituted aralkyl, optionally substituted aralkenyl, optionally substituted aralkynyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, optionally substituted heteroaryl, optionally substituted heteroarylalkyl, —Rb—ORa, —Rb—OC(O)—Ra, —Rb—OC(O)—ORa, —Rb—OC(O)—N(Ra)2, —Rb—N(Ra)2, —Rb—C(O)Ra, —Rb—C(O)ORa, —Rb—C(O)N(Ra)2, —Rb—O—Rc—C(O)N(Ra)2, —Rb—N(Ra)C(O)ORa, —Rb—N(Ra)C(O) Ra, —Rb—N(Ra)S(O)tRa (where t is 1 or 2), —Rb—S(O)tORa (where t is 1 or 2), —Rb—S(O)tRa (where t is 1 or 2) and —Rb—S(O)tN(Ra)2 (where t is 1 or 2), where each Ra is independently hydrogen, alkyl, fluoroalkyl, cycloalkyl, cycloalkylalkyl, aryl, aralkyl, heterocyclyl, heterocyclylalkyl, heteroaryl or heteroarylalkyl, each Rb is independently a direct bond or a straight or branched alkylene or alkenylene chain, and Rc is a straight or branched alkylene or alkenylene chain, and where each of the above substituents is unsubstituted unless otherwise indicated. The terms “heterocyclyl” and “heterocycle” are used interchangeably.
“Heterocyclylalkyl” refers to a radical of the formula —Rc-heterocyclyl where Rc is an alkylene chain as defined above. If the heterocyclyl is a nitrogen-containing heterocyclyl, the heterocyclyl is optionally attached to the alkyl radical at the nitrogen atom. The alkylene chain of the heterocyclylalkyl radical is optionally substituted as defined above for an alkylene chain. The heterocyclyl part of the heterocyclylalkyl radical is optionally substituted as defined above for a heterocyclyl group.
“Heterocyclylalkoxy” refers to a radical bonded through an oxygen atom of the formula —O—Rc-heterocyclyl where Rc is an alkylene chain as defined above. If the heterocyclyl is a nitrogen-containing heterocyclyl, the heterocyclyl is optionally attached to the alkyl radical at the nitrogen atom. The alkylene chain of the heterocyclylalkoxy radical is optionally substituted as defined above for an alkylene chain. The heterocyclyl part of the heterocyclylalkoxy radical is optionally substituted as defined above for a heterocyclyl group.
“Heteroaryl” refers to a radical derived from a 3- to 18-membered aromatic ring radical that comprises two to seventeen carbon atoms and from one to six heteroatoms selected from nitrogen, oxygen and sulfur. As used herein, the heteroaryl radical is a monocyclic, bicyclic, tricyclic or tetracyclic ring system, wherein at least one of the rings in the ring system is fully unsaturated, i.e., it contains a cyclic, delocalized (4n+2) π-electron system in accordance with the Hückel theory. Heteroaryl includes fused or bridged ring systems. The heteroatom(s) in the heteroaryl radical is optionally oxidized. One or more nitrogen atoms, if present, are optionally quaternized. The heteroaryl is attached to the rest of the molecule through any atom of the ring(s). Examples of heteroaryls include, but are not limited to, azepinyl, acridinyl, benzimidazolyl, benzindolyl, 1,3-benzodioxolyl, benzofuranyl, benzooxazolyl, benzo[d]thiazolyl, benzothiadiazolyl, benzo[b][1,4]dioxepinyl, benzo[b][1,4]oxazinyl, 1,4-benzodioxanyl, benzonaphthofuranyl, benzoxazolyl, benzodioxolyl, benzodioxinyl, benzopyranyl, benzopyranonyl, benzofuranyl, benzofuranonyl, benzothienyl (benzothiophenyl), benzothieno[3,2-d]pyrimidinyl, benzotriazolyl, benzo[4,6]imidazo[1,2-a]pyridinyl, carbazolyl, cinnolinyl, cyclopenta[d]pyrimidinyl, 6,7-dihydro-5H-cyclopenta[4,5]thieno[2,3-d]pyrimidinyl, 5,6-dihydrobenzo[h]quinazolinyl, 5,6-dihydrobenzo[h]cinnolinyl, 6,7-dihydro-5H-benzo[6,7]cyclohepta[1,2-c]pyridazinyl, dibenzofuranyl, dibenzothiophenyl, furanyl, furanonyl, furo[3,2-c]pyridinyl, 5,6,7,8,9,10-hexahydrocycloocta[d]pyrimidinyl, 5,6,7,8,9,10-hexahydrocycloocta[d]pyridazinyl, 5,6,7,8,9,10-hexahydrocycloocta[d]pyridinyl, isothiazolyl, imidazolyl, indazolyl, indolyl, indazolyl, isoindolyl, indolinyl, isoindolinyl, isoquinolyl, indolizinyl, isoxazolyl, 5,8-methano-5,6,7,8-tetrahydroquinazolinyl, naphthyridinyl, 1,6-naphthyridinonyl, oxadiazolyl, 2-oxoazepinyl, oxazolyl, oxiranyl, 5,6,6a,7,8,9,10,10a-octahydrobenzo[h]quinazolinyl, 1-phenyl-1H-pyrrolyl, phenazinyl, phenothiazinyl, phenoxazinyl, phthalazinyl, pteridinyl, purinyl, pyrrolyl, pyrazolyl, pyrazolo[3,4-d]pyrimidinyl, pyridinyl, pyrido[3,2-d]pyrimidinyl, pyrido[3,4-d]pyrimidinyl, pyrazinyl, pyrimidinyl, pyridazinyl, pyrrolyl, quinazolinyl, quinoxalinyl, quinolinyl, isoquinolinyl, tetrahydroquinolinyl, 5,6,7,8-tetrahydroquinazolinyl, 5,6,7,8-tetrahydrobenzo[4,5]thieno[2,3-d]pyrimidinyl, 6,7,8,9-tetrahydro-5H-cyclohepta[4,5]thieno[2,3-d]pyrimidinyl, 5,6,7,8-tetrahydropyrido[4,5-c]pyridazinyl, thiazolyl, thiadiazolyl, triazolyl, tetrazolyl, triazinyl, thieno[2,3-d]pyrimidinyl, thieno[3,2-d]pyrimidinyl, thieno[2,3-c]pridinyl, and thiophenyl (i.e. thienyl). Unless stated otherwise specifically in the specification, the term “heteroaryl” is meant to include heteroaryl radicals as defined above which are optionally substituted by one or more substituents selected from alkyl, alkenyl, alkynyl, halo, fluoroalkyl, haloalkenyl, haloalkynyl, oxo, thioxo, cyano, nitro, optionally substituted aryl, optionally substituted aralkyl, optionally substituted aralkenyl, optionally substituted aralkynyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, optionally substituted heteroaryl, optionally substituted heteroarylalkyl, —Rb—ORa, —Rb—OC(O)—Ra, —Rb—OC(O)—ORa, —Rb—OC(O)—N(Ra)2, —Rb—N(Ra)2, —Rb—C(O)Ra, —Rb—C(O)ORa, —Rb—C(O)N(Ra)2, —Rb—O—Rc—C(O)N(Ra)2, —Rb—N(Ra)C(O)ORa, —Rb—N(Ra)C(O) Ra, —Rb—N(Ra)S(O)tRa (where t is 1 or 2), —Rb—S(O)tORa (where t is 1 or 2), —Rb—S(O)tRa (where t is 1 or 2) and —Rb—S(O)tN(Ra)2 (where t is 1 or 2), where each Ra is independently hydrogen, alkyl, fluoroalkyl, cycloalkyl, cycloalkylalkyl, aryl, aralkyl, heterocyclyl, heterocyclylalkyl, heteroaryl or heteroarylalkyl, each Rb is independently a direct bond or a straight or branched alkylene or alkenylene chain, and Rc is a straight or branched alkylene or alkenylene chain, and where each of the above substituents is unsubstituted unless otherwise indicated.
“N-heteroaryl” refers to a heteroaryl radical as defined above containing at least one nitrogen and where the point of attachment of the heteroaryl radical to the rest of the molecule is through a nitrogen atom in the heteroaryl radical. An N-heteroaryl radical is optionally substituted as described above for heteroaryl radicals.
“C-heteroaryl” refers to a heteroaryl radical as defined above and where the point of attachment of the heteroaryl radical to the rest of the molecule is through a carbon atom in the heteroaryl radical. A C-heteroaryl radical is optionally substituted as described above for heteroaryl radicals.
“Heteroaryloxy” refers to radical bonded through an oxygen atom of the formula —O-heteroaryl, where heteroaryl is as defined above.
“Heteroarylalkyl” refers to a radical of the formula —Rc-heteroaryl, where Rc is an alkylene chain as defined above. If the heteroaryl is a nitrogen-containing heteroaryl, the heteroaryl is optionally attached to the alkyl radical at the nitrogen atom. The alkylene chain of the heteroarylalkyl radical is optionally substituted as defined above for an alkylene chain. The heteroaryl part of the heteroarylalkyl radical is optionally substituted as defined above for a heteroaryl group.
“Heteroarylalkoxy” refers to a radical bonded through an oxygen atom of the formula —O—Rc-heteroaryl, where Rc is an alkylene chain as defined above. If the heteroaryl is a nitrogen-containing heteroaryl, the heteroaryl is optionally attached to the alkyl radical at the nitrogen atom. The alkylene chain of the heteroarylalkoxy radical is optionally substituted as defined above for an alkylene chain. The heteroaryl part of the heteroarylalkoxy radical is optionally substituted as defined above for a heteroaryl group.
In some embodiments, he compounds disclosed herein contain one or more asymmetric centers and thus give rise to enantiomers, diastereomers, and other stereoisomeric forms that are defined, in terms of absolute stereochemistry, as (R)— or (S)—. Unless stated otherwise, it is intended that all stereoisomeric forms of the compounds disclosed herein are contemplated by this disclosure. When the compounds described herein contain alkene double bonds, and unless specified otherwise, it is intended that this disclosure includes both E and Z geometric isomers (e.g., cis or trans.) Likewise, all possible isomers, as well as their racemic and optically pure forms, and all tautomeric forms are also intended to be included. The term “geometric isomer” refers to E or Z geometric isomers (e.g., cis or trans) of an alkene double bond. The term “positional isomer” refers to structural isomers around a central ring, such as ortho-, meta-, and para-isomers around a benzene ring.
A “tautomer” refers to a molecule wherein a proton shift from one atom of a molecule to another atom of the same molecule is possible. In certain embodiments, the compounds presented herein exist as tautomers. In circumstances where tautomerization is possible, a chemical equilibrium of the tautomers will exist. The exact ratio of the tautomers depends on several factors, including physical state, temperature, solvent, and pH. Some examples of tautomeric equilibrium include:




“Optional” or “optionally” means that a subsequently described event or circumstance may or may not occur and that the description includes instances when the event or circumstance occurs and instances in which it does not. For example, “optionally substituted aryl” means that the aryl radical may or may not be substituted and that the description includes both substituted aryl radicals and aryl radicals having no substitution.
“Pharmaceutically acceptable salt” includes both acid and base addition salts. A pharmaceutically acceptable salt of any one of the piperazine carbamates described herein is intended to encompass any and all pharmaceutically suitable salt forms. Preferred pharmaceutically acceptable salts of the compounds described herein are pharmaceutically acceptable acid addition salts and pharmaceutically acceptable base addition salts.
“Pharmaceutically acceptable acid addition salt” refers to those salts which retain the biological effectiveness and properties of the free bases, which are not biologically or otherwise undesirable, and which are formed with inorganic acids such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, hydroiodic acid, hydrofluoric acid, phosphorous acid, and the like. Also included are salts that are formed with organic acids such as aliphatic mono- and dicarboxylic acids, phenyl-substituted alkanoic acids, hydroxy alkanoic acids, alkanedioic acids, aromatic acids, aliphatic and. aromatic sulfonic acids, etc. and include, for example, acetic acid, trifluoroacetic acid, propionic acid, glycolic acid, pyruvic acid, oxalic acid, maleic acid, malonic acid, succinic acid, fumaric acid, tartaric acid, citric acid, benzoic acid, cinnamic acid, mandelic acid, methanesulfonic acid, ethanesulfonic acid, p-toluenesulfonic acid, salicylic acid, and the like. Exemplary salts thus include sulfates, pyrosulfates, bisulfates, sulfites, bisulfites, nitrates, phosphates, monohydrogenphosphates, dihydrogenphosphates, metaphosphates, pyrophosphates, chlorides, bromides, iodides, acetates, trifluoroacetates, propionates, caprylates, isobutyrates, oxalates, malonates, succinate suberates, sebacates, fumarates, maleates, mandelates, benzoates, chlorobenzoates, methylbenzoates, dinitrobenzoates, phthalates, benzenesulfonates, toluenesulfonates, phenylacetates, citrates, lactates, malates, tartrates, methanesulfonates, and the like. Also contemplated are salts of amino acids, such as arginates, gluconates, and galacturonates (see, for example, Berge S. M. et al., “Pharmaceutical Salts,” Journal of Pharmaceutical Science, 66:1-19 (1997)). Acid addition salts of basic compounds are prepared by contacting the free base forms with a sufficient amount of the desired acid to produce the salt.
“Pharmaceutically acceptable base addition salt” refers to those salts that retain the biological effectiveness and properties of the free acids, which are not biologically or otherwise undesirable. These salts are prepared from addition of an inorganic base or an organic base to the free acid. In some embodiments, pharmaceutically acceptable base addition salts are formed with metals or amines, such as alkali and alkaline earth metals or organic amines. Salts derived from inorganic bases include, but are not limited to, sodium, potassium, lithium, ammonium, calcium, magnesium, iron, zinc, copper, manganese, aluminum salts and the like. Salts derived from organic bases include, but are not limited to, salts of primary, secondary, and tertiary amines, substituted amines including naturally occurring substituted amines, cyclic amines and basic ion exchange resins, for example, isopropylamine, trimethylamine, diethylamine, triethylamine, tripropylamine, ethanolamine, diethanolamine, 2-dimethylaminoethanol, 2-diethylaminoethanol, dicyclohexylamine, lysine, arginine, histidine, caffeine, procaine, N,N-dibenzylethylenediamine, chloroprocaine, hydrabamine, choline, betaine, ethylenediamine, ethylenedianiline, N-methylglucamine, glucosamine, methylglucamine, theobromine, purines, piperazine, piperidine, N-ethylpiperidine, polyamine resins and the like. See Berge et al., supra.
As used herein, “treatment” or “treating” or “palliating” or “ameliorating” are used interchangeably herein. These terms refers to an approach for obtaining beneficial or desired results including but not limited to therapeutic benefit and/or a prophylactic benefit. By “therapeutic benefit” is meant eradication or amelioration of the underlying disorder being treated. Also, a therapeutic benefit is achieved with the eradication or amelioration of one or more of the physiological symptoms associated with the underlying disorder such that an improvement is observed in the patient, notwithstanding that the patient is still afflicted with the underlying disorder. For prophylactic benefit, the compositions are administered to a patient at risk of developing a particular disease, or to a patient reporting one or more of the physiological symptoms of a disease, even though a diagnosis of this disease has not been made.
Compounds
Piperazine carbamates are described herein which are modulators of MAGL and/or ABHD6. These compounds, and compositions comprising these compounds, are useful for the treatment of pain.
One embodiment provides a compound of Formula (I):




wherein:

    R1 is halogen, —OR3, —CN, aryl (optionally substituted by one, two, or three moieties each independently selected from R4), aryloxy (optionally substituted by one, two, or three moieties each independently selected from R4), heteroaryl (optionally substituted by one, two, or three moieties each independently selected from R4), heteroaryloxy (optionally substituted by one, two, or three moieties each independently selected from R4), heterocycle (optionally substituted by one, two, or three moieties each independently selected from R4), cycloalkyl (optionally substituted by one, two, or three moieties each independently selected from R4), optionally substituted C1-6 alkyl, optionally substituted C2-6 alkenyl, optionally substituted C2-6 alkynyl, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, —NR8C(O)NR9R10, —C(O)R9, —C(O)OR9, —C(O)NR9R10, —S(O)wR11, or —SH;
    R2 is —NR5R6, -alkyl(NR14R15), or —OR7;
    each R3 is independently selected from H, C1-6 alkyl, haloalkyl, and aminoalkyl;
    each R4 is independently selected from halogen, —OR3, —CN, nitro, optionally substituted C1-6 alkyl, optionally substituted C1-6 alkenyl, optionally substituted C1-6 alkynyl, cycloalkyl, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, —NR8C(O)NR9R10, —C(O)R9, —C(O)OR9, —C(O)NR9R10, —S(O)wR11, and —SH;
    R5 and R6, together with the nitrogen to which they are attached, form
    
        (i) a monocyclic heterocycle, a fused bicyclic heterocycle, or a spirocycle; or
        (ii) a 7-8 membered bridged heterocyclic ring optionally containing an additional O, N, or S;
        wherein the monocyclic heterocycle, the fused bicyclic heterocycle, or the spirocycle is substituted with one or more substituents independently selected from optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocycle, optionally substituted cycloalkyl, haloalkyl, —OR7, —NR12R13, —C(O)OR9, —C(O)NR9R10, —NR8SO2R9, —NR8C(O)OR9, and —NR8C(O)NR9R10; and the monocyclic heterocycle, the fused bicyclic heterocycle, or the spirocycle optionally contains an additional O, N, or S; and
        the 7-8 membered bridged heterocyclic ring is optionally substituted with one or more substituents independently selected from halogen, —CN, oxo, optionally substituted C1-6 alkyl, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocycle, optionally substituted cycloalkyl, —S(O)wR11, —OR3, —OR7, —C(O)R9, —C(O)OR9, —C(O)NR9R10, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, and —NR8C(O)NR9R10;
    
    
    R7 is alkynyl or (alkynyl)alkyl;
    each R8 is independently selected from H, optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl;
    each R9 and R10 is independently selected from H, optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl; or R9 and R10 together with the nitrogen to which they are attached form an optionally substituted heterocycle optionally containing an additional O, N, or S;
    each R11 is independently selected from optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl;
    R12 and R13 together with the nitrogen to which they are attached form an optionally substituted heterocycle optionally containing an additional O, N, or S;
    R14 and R15 together with the nitrogen to which they are attached form an optionally substituted heterocycle optionally containing an additional O, N, or S; and
    w is 0, 1, or 2;

or a solvate, hydrate, tautomer, N-oxide, or pharmaceutically acceptable salt thereof.



In some embodiments of a compound of Formula (I), R2 is —NR5R6. In some embodiments of a compound of Formula (I), R5 and R6, together with the nitrogen to which they are attached, form a monocyclic heterocycle, a fused bicyclic heterocycle, or a spirocycle, wherein: the monocyclic heterocycle, the fused bicyclic heterocycle, or the spirocycle is substituted with one or more substituents independently selected from optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocycle, optionally substituted cycloalkyl, haloalkyl, —OR7, —NR12R13, —C(O)OR9, —C(O)NR9R10, —NR8SO2R9, —NR8C(O)OR9, and —NR8C(O)NR9R10; and monocyclic heterocycle, the fused bicyclic heterocycle, or the spirocycle optionally contains an additional O, N, or S. In some embodiments of a compound of Formula (I), R12 and R13 together with the nitrogen to which they are attached form a heterocycle optionally containing an additional O, N, or S; wherein the heterocycle is substituted with one or more substituents independently selected from halogen, oxo, —OR3, —CN, aryl (optionally substituted by one, two, or three moieties each independently selected from R4), aryloxy (optionally substituted by one, two, or three moieties each independently selected from R4), heteroaryl (optionally substituted by one, two, or three moieties each independently selected from R4), heteroaryloxy (optionally substituted by one, two, or three moieties each independently selected from R4), heterocycle (optionally substituted by one, two, or three moieties each independently selected from R4), cycloalkyl (optionally substituted by one, two, or three moieties each independently selected from R4), optionally substituted C1-6 alkyl, optionally substituted C2-6 alkenyl, optionally substituted C2-6 alkynyl, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, —S(O)wR11, —NR8C(O)NR9R10, —C(O)R9, —C(O)OR9, and —C(O)NR9R10. In some embodiments of a compound of Formula (I), R12 and R13 together with the nitrogen to which they are attached form a heterocycle optionally containing an additional O, N, or S; wherein the heterocycle is substituted with one or more substituents independently selected from halogen, oxo, —OR3, —CN, aryl, aryloxy, heteroaryl, heteroaryloxy, heterocycle, cycloalkyl, haloalkyl, C1-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, —S(O)wR11, —NR8C(O)NR9R10, —C(O)R9, —C(O)OR9, and —C(O)NR9R10. In some embodiments of a compound of Formula (I), R12 and R13 together with the nitrogen to which they are attached form a heterocycle optionally containing an additional O, N, or S; wherein the heterocycle is substituted with one or more substituents independently selected from halogen, oxo, —OR3, —CN, aryl, aryloxy, haloalkyl, C1-6 alkyl, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, —S(O)wR11, —NR8C(O)NR9R10, —C(O)R9, —C(O)OR9, and —C(O)NR9R10.
In some embodiments of a compound of Formula (I), R2 is —NR5R6. In some embodiments of a compound of Formula (I), R5 and R6, together with the nitrogen to which they are attached, form a monocyclic heterocycle wherein the monocyclic heterocycle is substituted with one or more substituents independently selected from optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocycle, optionally substituted cycloalkyl, haloalkyl, —OR7, —NR12R13, —C(O)OR9, —C(O)NR9R10, —NR8SO2R9, —NR8C(O)OR9, and —NR8C(O)NR9R10; and the monocyclic heterocycle optionally contains an additional O, N, or S. In some embodiments of a compound of Formula (I), R5 and R6, together with the nitrogen to which they are attached, form a monocyclic heterocycle substituted with one or more substituents independently selected from optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocycle, optionally substituted cycloalkyl, haloalkyl, —OR7, —NR12R13, —C(O)OR9, —C(O)NR9R10, —NR8SO2R9, —NR8C(O)OR9, and —NR8C(O)NR9R10, wherein the monocyclic heterocycle is selected from azetidine, pyrrolidine, piperidine, and morpholine. In some embodiments of a compound of Formula (I), R5 and R6, together with the nitrogen to which they are attached, form a monocyclic heterocycle substituted with one substituent selected from haloalkyl, —OR7, —C(O)OR9, —C(O)NR9R10, and —NR8SO2R9, wherein the monocyclic heterocycle is selected from azetidine, pyrrolidine, piperidine, and morpholine. In some embodiments of a compound of Formula (I), R5 and R6, together with the nitrogen to which they are attached, form a monocyclic heterocycle substituted with one substituent selected from haloalkyl, —OR7, —C(O)OR9, —C(O)NR9R10, and —NR8SO2R9, wherein the monocyclic heterocycle is selected from pyrrolidine, piperidine, and morpholine. In some embodiments of a compound of Formula (I), R5 and R6, together with the nitrogen to which they are attached, form a monocyclic heterocycle substituted with one substituent selected from —C(O)OR9 and —C(O)NR9R10, wherein the monocyclic heterocycle is selected from pyrrolidine, piperidine, and morpholine. In some embodiments of a compound of Formula (I), R5 and R6, together with the nitrogen to which they are attached, form a monocyclic heterocycle substituted with one substituent selected from —C(O)OR9 and —C(O)NR9R10, wherein the monocyclic heterocycle is selected from pyrrolidine, piperidine, and morpholine, and R9 and R10 is independently selected from H and unsubstituted alkyl. In some embodiments of a compound of Formula (I), R5 and R6, together with the nitrogen to which they are attached, form a monocyclic heterocycle substituted with one substituent selected from —C(O)OR9 and —C(O)NR9R10, wherein the monocyclic heterocycle is pyrrolidine and R9 and R10 is independently selected from H and unsubstituted alkyl. In some embodiments of a compound of Formula (I), R5 and R6, together with the nitrogen to which they are attached, form a monocyclic heterocycle substituted with one substituent selected from —C(O)OR9 and —C(O)NR9R10, wherein the monocyclic heterocycle is piperidine and R9 and R10 is independently selected from H and unsubstituted alkyl. In some embodiments of a compound of Formula (I), R5 and R6, together with the nitrogen to which they are attached, form a monocyclic heterocycle substituted with one substituent selected from —C(O)OR9 and —C(O)NR9R10, wherein the monocyclic heterocycle is morpholine and R9 and R10 is independently selected from H and unsubstituted alkyl. In some embodiments of a compound of Formula (I), R5 and R6, together with the nitrogen to which they are attached, form a monocyclic heterocycle substituted with one or more substituents independently selected from substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heterocycle, substituted or unsubstituted cycloalkyl, haloalkyl, —OR7, —NR12R13, —C(O)OR9, —C(O)NR9R10, —NR8SO2R9, —NR8C(O)OR9, and —NR8C(O)NR9R10, wherein the monocyclic heterocycle is azetidine. In some embodiments of a compound of Formula (I), R5 and R6, together with the nitrogen to which they are attached, form a monocyclic heterocycle substituted with one or more substituents independently selected from substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heterocycle, substituted or unsubstituted cycloalkyl, haloalkyl, —OR7, —NR12R13, —C(O)OR9, —C(O)NR9R10, —NR8SO2R9, —NR8C(O)OR9, and —NR8C(O)NR9R10, wherein the monocyclic heterocycle is pyrrolidine. In some embodiments of a compound of Formula (I), R5 and R6, together with the nitrogen to which they are attached, form a monocyclic heterocycle substituted with one or more substituents independently selected from substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heterocycle, substituted or unsubstituted cycloalkyl, haloalkyl, —OR7, —NR12R13, —C(O)OR9, —C(O)NR9R10, —NR8SO2R9, —NR8C(O)OR9, and —NR8C(O)NR9R10, wherein the monocyclic heterocycle is piperidine. In some embodiments of a compound of Formula (I), R5 and R6, together with the nitrogen to which they are attached, form a monocyclic heterocycle substituted with one or more substituents independently selected from substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heterocycle, substituted or unsubstituted cycloalkyl, haloalkyl, —OR7, —NR12R13, —C(O)OR9, —C(O)NR9R10, —NR8SO2R9, —NR8C(O)OR9, and —NR8C(O)NR9R10, wherein the monocyclic heterocycle is morpholine.
In some embodiments of a compound of Formula (I), R2 is —NR5R6. In some embodiments of a compound of Formula (I), R5 and R6, together with the nitrogen to which they are attached, form a fused bicyclic heterocycle wherein the fused bicyclic heterocycle is substituted with one or more substituents independently selected from optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocycle, optionally substituted cycloalkyl, haloalkyl, —OR7, —NR12R13, —C(O)OR9, —C(O)NR9R10, —NR8SO2R9, —NR8C(O)OR9, and —NR8C(O)NR9R10; and the fused bicyclic heterocycle optionally contains an additional O, N, or S. In some embodiments of a compound of Formula (I), R5 and R6, together with the nitrogen to which they are attached, form




wherein R17 is optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocycle, optionally substituted cycloalkyl, haloalkyl, —C(O)OR9, or —C(O)NR9R10. In further embodiments of a compound of Formula (I), R17 is aryl, heteroaryl, heterocycle, cycloalkyl, haloalkyl, —C(O)OR9, or —C(O)NR9R10. In still further embodiments of a compound of Formula (I), R17 is cycloalkyl, haloalkyl, —C(O)OR9, or —C(O)NR9R10.

In some embodiments of a compound of Formula (I), R2 is —NR5R6. In some embodiments of a compound of Formula (I), R5 and R6, together with the nitrogen to which they are attached, form a spirocycle wherein the spirocycle is substituted with one or more substituents independently selected from optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocycle, optionally substituted cycloalkyl, haloalkyl, —OR7, —NR12R13, —C(O)OR9, —C(O)NR9R10, —NR8SO2R9, —NR8C(O)OR9, and —NR8C(O)NR9R10; and the spirocycle optionally contains an additional O, N, or S. In some embodiments of a compound of Formula (I), R5 and R6, together with the nitrogen to which they are attached, form a spirocycle selected from




wherein R17 is optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocycle, optionally substituted cycloalkyl, haloalkyl, —C(O)OR9, or —C(O)NR9R10. In further embodiments, R17 is aryl, heteroaryl, heterocycle, cycloalkyl, haloalkyl, —C(O)OR9, or —C(O)NR9R10. In still further embodiments, R17 is cycloalkyl, haloalkyl, —C(O)OR9, or —C(O)NR9R10.

In some embodiments of a compound of Formula (I), R2 is —NR5R6. In some embodiments of a compound of Formula (I), R5 and R6, together with the nitrogen to which they are attached, form a 7-8 membered bridged heterocyclic ring optionally containing an additional O, N, or S, and optionally substituted with one or more substituents independently selected from halogen, —CN, oxo, optionally substituted C1-6 alkyl, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocycle, optionally substituted cycloalkyl, —S(O)wR11, —OR3, —OR7, —C(O)R9, —C(O)OR9, —C(O)NR9R10, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, and —NR8C(O)NR9R10. In some embodiments of a compound of Formula (I), R5 and R6, together with the nitrogen to which they are attached, form a 7-8 membered bridged heterocyclic ring containing an additional O, N, or S. In some embodiments of a compound of Formula (I), R5 and R6, together with the nitrogen to which they are attached, form an 8-membered bridged heterocyclic ring selected from and




In some embodiments of a compound of Formula (I), R2 is -alkyl(NR14R15). In some embodiments of a compound of Formula (I), R14 and R15, together with the nitrogen to which they are attached, form a heterocycle optionally containing an additional O, N, or S; wherein the heterocycle is substituted with one or more substituents independently selected from halogen, oxo, —OR3, —CN, aryl (optionally substituted by one, two, or three moieties each independently selected from R4), aryloxy (optionally substituted by one, two, or three moieties each independently selected from R4), heteroaryl (optionally substituted by one, two, or three moieties each independently selected from R4), heteroaryloxy (optionally substituted by one, two, or three moieties each independently selected from R4), heterocycle (optionally substituted by one, two, or three moieties each independently selected from R4), cycloalkyl (optionally substituted by one, two, or three moieties each independently selected from R4), optionally substituted C1-6 alkyl, optionally substituted C2-6 alkenyl, optionally substituted C2-6 alkynyl, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, —S(O)wR11, —NR8C(O)NR9R10, —C(O)R9, —C(O)OR9, and —C(O)NR9R10. In some embodiments of a compound of Formula (I), R14 and R15, together with the nitrogen to which they are attached, form a substituted heterocycle; wherein the substituted heterocycle has one or more substituents independently selected from halogen, oxo, —OR3, —CN, C1-6 alkyl, haloalkyl, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, —S(O)wR11, —NR8C(O)NR9R10, —C(O)R9, —C(O)OR9, and —C(O)NR9R10. In some embodiments of a compound of Formula (I), R14 and R15, together with the nitrogen to which they are attached, form an optionally substituted pyrrolidine, optionally substituted piperidine, optionally substituted morpholine, or optionally substituted piperazine. In some embodiments of a compound of Formula (I), R14 and R15 together with the nitrogen to which they are attached, form a substituted or unsubstituted pyrrolidine. In some embodiments of a compound of Formula (I), R14 and R15, together with the nitrogen to which they are attached, form a substituted or unsubstituted piperidine. In some embodiments of a compound of Formula (I), R14 and R15, together with the nitrogen to which they are attached, form a substituted or unsubstituted morpholine. In some embodiments of a compound of Formula (I), R14 and R15, together with the nitrogen to which they are attached, form a substituted or unsubstituted piperazine. In some embodiments of a compound of Formula (I), R14 and R15, together with the nitrogen to which they are attached, form an optionally substituted heterocycle selected from:




In some embodiments of a compound of Formula (I), R2 is —OR7.
In some embodiments of a compound of Formula (I), R1 is halogen, —CH3, —CF3, —OCH3, or —OCF3. In some embodiments of a compound of Formula (I), R1 is halogen. In some embodiments of a compound of Formula (I), R1 is —Cl. In some embodiments of a compound of Formula (I), R1 is —CH3. In some embodiments of a compound of Formula (I), R1 is —CF3. In some embodiments of a compound of Formula (I), R1 is —OCH3. In some embodiments of a compound of Formula (I), R1 is —OCF3.
Another embodiment provides a compound of Formula (Ia):




wherein:

    R1 is halogen, —OR3, —CN, aryl (optionally substituted by one, two, or three moieties each independently selected from R4), aryloxy (optionally substituted by one, two, or three moieties each independently selected from R4), heteroaryl (optionally substituted by one, two, or three moieties each independently selected from R4), heteroaryloxy (optionally substituted by one, two, or three moieties each independently selected from R4), heterocycle (optionally substituted by one, two, or three moieties each independently selected from R4), cycloalkyl (optionally substituted by one, two, or three moieties each independently selected from R4), optionally substituted C1-6 alkyl, optionally substituted C2-6 alkenyl, optionally substituted C2-6 alkynyl, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, —NR8C(O)NR9R10, —C(O)R9, —C(O)OR9, —C(O)NR9R10, —S(O)wR11, or —SH;
    R2 is —C(O)C(O)OR7;
    each R3 is independently selected from H, C1-6 alkyl, haloalkyl, and aminoalkyl;
    each R4 is independently selected from halogen, —OR3, —CN, nitro, optionally substituted C1-6 alkyl, optionally substituted C1-6 alkenyl, optionally substituted C1-6 alkynyl, cycloalkyl, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, —NR8C(O)NR9R10, —C(O)R9, —C(O)OR9, —C(O)NR9R10, —S(O)wR11, and —SH;
    R7 is H or C1-6 alkyl;
    each R8 is independently selected from H, optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl;
    each R9 and R10 is independently selected from H, optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl; or R9 and R10 together with the nitrogen to which they are attached form an optionally substituted heterocycle optionally containing an additional O, N, or S;
    each R11 is independently selected from optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl; and
    w is 0, 1, or 2;

or a solvate, hydrate, tautomer, N-oxide, or pharmaceutically acceptable salt thereof.



In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7 and R7 is H. In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7, R7 is H, and R1 is optionally substituted C1-6 alkyl. In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7, R7 is H, and R1 is halogen, —CH3, —CF3, —OCH3, or —OCF3. In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7, R7 is H, and R1 is —CH3. In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7, R7 is H, and R1 is —CF3. In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7, R7 is H, and R1 is halogen. In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7, R7 is H, and R1 is —Cl. In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7, R7 is H, and R1 is —OCH3. In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7, R7 is H, and R1 is —OCF3.
In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7 and R7 is C1-6 alkyl. In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7, R7 is C1-6 alkyl, and R1 is optionally substituted C1-6 alkyl. In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7, R7 is C1-6 alkyl, and R1 is halogen, —CH3, —CF3, —OCH3, or —OCF3. In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7, R7 is C1-6 alkyl, and R1 is —CH3. In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7, R7 is C1-6 alkyl, and R1 is CF3. In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7, R7 is C1-6 alkyl, and R1 is halogen. In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7, R7 is C1-6 alkyl, and R1 is —Cl. In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7, R7 is C1-6 alkyl, and R1 is —OCH3. In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7, R7 is C1-6 alkyl, and R1 is —OCF3.
In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7 and R7 is —CH3. In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7, R7 is —CH3, and R1 is optionally substituted C1-6 alkyl. In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7, R7 is —CH3, and R1 is halogen, —CH3, —CF3, —OCH3, or —OCF3. In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7, R7 is —CH3, and R1 is —CH3. In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7, R7 is —CH3, and R1 is CF3. In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7, R7 is —CH3, and R1 is halogen. In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7, R7 is —CH3, and R1 is —Cl. In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7, R7 is —CH3, and R1 is —OCH3. In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7, R7 is —CH3, and R1 is —OCF3.
In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7 and R7 is —CH2CH3. In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7, R7 is —CH2CH3, and R1 is optionally substituted C1-6 alkyl. In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7, R7 is —CH2CH3, and R1 is halogen, —CH3, —CF3, —OCH3, or —OCF3. In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7, R7 is —CH2CH3, and R1 is —CH3. In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7, R7 is —CH2CH3, and R1 is CF3. In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7, R7 is —CH2CH3, and R1 is halogen. In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7, R7 is —CH2CH3, and R1 is —Cl. In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7, R7 is —CH2CH3, and R1 is —OCH3. In some embodiments of a compound of Formula (Ia), R2 is —C(O)C(O)OR7, R7 is —CH2CH3, and R1 is —OCF3 
Another embodiment provides a compound of Formula (II):




wherein:

    R1 is halogen, —OR3, —CN, aryl (optionally substituted by one, two, or three moieties each independently selected from R4), aryloxy (optionally substituted by one, two, or three moieties each independently selected from R4), heteroaryl (optionally substituted by one, two, or three moieties each independently selected from R4), heteroaryloxy (optionally substituted by one, two, or three moieties each independently selected from R4), heterocycle (optionally substituted by one, two, or three moieties each independently selected from R4), cycloalkyl (optionally substituted by one, two, or three moieties each independently selected from R4), optionally substituted C1-6 alkyl, optionally substituted C2-6 alkenyl, optionally substituted C2-6 alkynyl, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, —NR8C(O)NR9R10, —C(O)R9, —C(O)OR9, —C(O)NR9R10, —S(O)wR11, or —SH;
    each R3 is independently selected from H, C1-6 alkyl, haloalkyl, and aminoalkyl;
    each R4 is independently selected from halogen, —OR3, —CN, nitro, optionally substituted C1-6 alkyl, optionally substituted C1-6 alkenyl, optionally substituted C1-6 alkynyl, cycloalkyl, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, —NR8C(O)NR9R10, —C(O)R9, —C(O)OR9, —C(O)NR9R10, —S(O)wR11, and —SH;
    R5 and R6, together with the nitrogen to which they are attached, form
    
        (i) a monocyclic heterocycle, a fused bicyclic heterocycle, or a spirocycle; or
        (ii) a 7-8 membered bridged heterocyclic ring optionally containing an additional O, N, or S;
        wherein the monocyclic heterocycle, the fused bicyclic heterocycle, or the spirocycle is substituted with one or more substituents independently selected from optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocycle, optionally substituted cycloalkyl, haloalkyl, —OR7, —NR12R13, —C(O)OR9, —C(O)NR9R10, —NR8SO2R9, —NR8C(O)OR9, and —NR8C(O)NR9R10; and the monocyclic heterocycle, the fused bicyclic heterocycle, or the spirocycle optionally contains an additional O, N, or S; and
        the 7-8 membered bridged heterocyclic ring is optionally substituted with one or more substituents independently selected from halogen, —CN, oxo, optionally substituted C1-6 alkyl, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocycle, optionally substituted cycloalkyl, —S(O)wR11, —OR3, —OR7, —C(O)R9, —C(O)OR9, —C(O)NR9R10, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, and —NR8C(O)NR9R10;
    
    
    R7 is alkynyl or (alkynyl)alkyl;
    each R8 is independently selected from H, optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl;
    each R9 and R10 is independently selected from H, optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl; or R9 and R10 together with the nitrogen to which they are attached form an optionally substituted heterocycle optionally containing an additional O, N, or S;
    each R11 is independently selected from optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl;
    R12 and R13 together with the nitrogen to which they are attached form an optionally substituted heterocycle optionally containing an additional O, N, or S; and
    w is 0, 1, or 2;

or a solvate, hydrate, tautomer, N-oxide, or pharmaceutically acceptable salt thereof.



In some embodiments of a compound of Formula (II), R5 and R6, together with the nitrogen to which they are attached, form a monocyclic heterocycle, a fused bicyclic heterocycle, or a spirocycle, wherein the monocyclic heterocycle, the fused bicyclic heterocycle, or the spirocycle is substituted with one or more substituents independently selected from optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocycle, optionally substituted cycloalkyl, haloalkyl, —OR7, —NR12R13, —C(O)OR9, —C(O)NR9R10, —NR8SO2R9, —NR8C(O)OR9, and —NR8C(O)NR9R10; and the monocyclic heterocycle, the fused bicyclic heterocycle, or the spirocycle optionally contains an additional O, N, or S. In some embodiments of a compound of Formula (II), R12 and R13 together with the nitrogen to which they are attached form a heterocycle optionally containing an additional O, N, or S; wherein the heterocycle is substituted with one or more substituents independently selected from halogen, oxo, —OR3, —CN, aryl (optionally substituted by one, two, or three moieties each independently selected from R4), aryloxy (optionally substituted by one, two, or three moieties each independently selected from R4), heteroaryl (optionally substituted by one, two, or three moieties each independently selected from R4), heteroaryloxy (optionally substituted by one, two, or three moieties each independently selected from R4), heterocycle (optionally substituted by one, two, or three moieties each independently selected from R4), cycloalkyl (optionally substituted by one, two, or three moieties each independently selected from R4), optionally substituted C1-6 alkyl, optionally substituted C2-6 alkenyl, optionally substituted C2-6 alkynyl, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, —S(O)wR11, —NR8C(O)NR9R10, —C(O)R9, —C(O)OR9, and —C(O)NR9R10. In some embodiments of a compound of Formula (II), R12 and R13 together with the nitrogen to which they are attached form a heterocycle optionally containing an additional O, N, or S; wherein the heterocycle is substituted with one or more substituents independently selected from halogen, oxo, —OR3, —CN, aryl, aryloxy, heteroaryl, heteroaryloxy, heterocycle, cycloalkyl, haloalkyl, C1-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, —S(O)wR11, —NR8C(O)NR9R10, —C(O)R9, —C(O)OR9, and —C(O)NR9R10. In some embodiments of a compound of Formula (II), R12 and R13 together with the nitrogen to which they are attached form a heterocycle optionally containing an additional O, N, or S; wherein the heterocycle is substituted with one or more substituents independently selected from halogen, oxo, —OR3, —CN, aryl, aryloxy, haloalkyl, C1-6 alkyl, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, —S(O)wR11, —NR8C(O)NR9R10, —C(O)R9, —C(O)OR9, and —C(O)NR9R10.
In some embodiments of a compound of Formula (II), R5 and R6, together with the nitrogen to which they are attached, form a monocyclic heterocycle wherein the monocyclic heterocycle is substituted with one or more substituents independently selected from optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocycle, optionally substituted cycloalkyl, haloalkyl, —OR7, —NR12R13, —C(O)OR9, —C(O)NR9R10, —NR8SO2R9, —NR8C(O)OR9, and —NR8C(O)NR9R10; and the monocyclic heterocycle optionally contains an additional O, N, or S. In some embodiments of a compound of Formula (II), R5 and R6, together with the nitrogen to which they are attached, form a monocyclic heterocycle substituted with one or more substituents independently selected from optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocycle, optionally substituted cycloalkyl, haloalkyl, —OR7, —NR12R13, —C(O)OR9, —C(O)NR9R10, —NR8SO2R9, —NR8C(O)OR9, and —NR8C(O)NR9R10, wherein the monocyclic heterocycle is selected from azetidine, pyrrolidine, piperidine, and morpholine. In some embodiments of a compound of Formula (II), R5 and R6, together with the nitrogen to which they are attached, form a monocyclic heterocycle substituted with one substituent selected from haloalkyl, —OR7, —C(O)OR9, —C(O)NR9R10, and —NR8SO2R9, wherein the monocyclic heterocycle is selected from azetidine, pyrrolidine, piperidine, and morpholine. In some embodiments of a compound of Formula (II), R5 and R6, together with the nitrogen to which they are attached, form a monocyclic heterocycle substituted with one substituent selected from haloalkyl, —OR7, —C(O)OR9, —C(O)NR9R10, and —NR8SO2R9, wherein the monocyclic heterocycle is selected from pyrrolidine, piperidine, and morpholine. In some embodiments of a compound of Formula (II), R5 and R6, together with the nitrogen to which they are attached, form a monocyclic heterocycle substituted with one substituent selected from —C(O)OR9 and —C(O)NR9R10, wherein the monocyclic heterocycle is selected from pyrrolidine, piperidine, and morpholine. In some embodiments of a compound of Formula (II), R5 and R6, together with the nitrogen to which they are attached, form a monocyclic heterocycle substituted with one substituent selected from —C(O)OR9 and —C(O)NR9R10, wherein the monocyclic heterocycle is selected from pyrrolidine, piperidine, and morpholine, and R9 and R10 is independently selected from H and unsubstituted alkyl. In some embodiments of a compound of Formula (II), R5 and R6, together with the nitrogen to which they are attached, form a monocyclic heterocycle substituted with one substituent selected from —C(O)OR9 and —C(O)NR9R10, wherein the monocyclic heterocycle is pyrrolidine and R9 and R10 is independently selected from H and unsubstituted alkyl. In some embodiments of a compound of Formula (II), R5 and R6, together with the nitrogen to which they are attached, form a monocyclic heterocycle substituted with one substituent selected from —C(O)OR9 and —C(O)NR9R10, wherein the monocyclic heterocycle is piperidine and R9 and R10 is independently selected from H and unsubstituted alkyl. In some embodiments of a compound of Formula (II), R5 and R6, together with the nitrogen to which they are attached, form a monocyclic heterocycle substituted with one substituent selected from —C(O)OR9 and —C(O)NR9R10, wherein the monocyclic heterocycle is morpholine and R9 and R10 is independently selected from H and unsubstituted alkyl. In some embodiments of a compound of Formula (II), R5 and R6, together with the nitrogen to which they are attached, form a monocyclic heterocycle substituted with one or more substituents independently selected from optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocycle, optionally substituted cycloalkyl, haloalkyl, —OR7, —NR12R13, —C(O)OR9, —C(O)NR9R10, —NR8SO2R9, —NR8C(O)OR9, and —NR8C(O)NR9R10, wherein the monocyclic heterocycle is azetidine. In some embodiments of a compound of Formula (II), R5 and R6, together with the nitrogen to which they are attached, form a monocyclic heterocycle substituted with one or more substituents independently selected from optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocycle, optionally substituted cycloalkyl, haloalkyl, —OR7, —NR12R13, —C(O)OR9, —C(O)NR9R10, —NR8SO2R9, —NR8C(O)OR9, and —NR8C(O)NR9R10, wherein the monocyclic heterocycle is pyrrolidine. In some embodiments of a compound of Formula (II), R5 and R6, together with the nitrogen to which they are attached, form a monocyclic heterocycle substituted with one or more substituents independently selected from optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocycle, optionally substituted cycloalkyl, haloalkyl, —OR7, —NR12R13, —C(O)OR9, —C(O)NR9R10, —NR8SO2R9, —NR8C(O)OR9, and —NR8C(O)NR9R10, wherein the monocyclic heterocycle is piperidine. In some embodiments of a compound of Formula (II), R5 and R6, together with the nitrogen to which they are attached, form a monocyclic heterocycle substituted with one or more substituents independently selected from optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocycle, optionally substituted cycloalkyl, haloalkyl, —OR7, —NR12R13, —C(O)OR9, —C(O)NR9R10, —NR8SO2R9, —NR8C(O)OR9, and —NR8C(O)NR9R10, wherein the monocyclic heterocycle is morpholine.
In some embodiments of a compound of Formula (II), R5 and R6, together with the nitrogen to which they are attached, form a fused bicyclic heterocycle wherein the fused bicyclic heterocycle is substituted with one or more substituents independently selected from optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocycle, optionally substituted cycloalkyl, haloalkyl, —OR7, —NR12R13, —C(O)OR9, —C(O)NR9R10, —NR8SO2R9, —NR8C(O)OR9, and —NR8C(O)NR9R10; and the fused bicyclic heterocycle optionally contains an additional O, N, or S. In some embodiments of a compound of Formula (II), R5 and R6, together with the nitrogen to which they are attached, form




wherein R is optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocycle, optionally substituted cycloalkyl, haloalkyl, —C(O)OR9, or —C(O)NR9R10. In further embodiments, R17 is aryl, heteroaryl, heterocycle, cycloalkyl, haloalkyl, —C(O)OR9, or —C(O)NR9R10. In still further embodiments, R17 is cycloalkyl, haloalkyl, —C(O)OR9, or —C(O)NR9R10.

In some embodiments of a compound of Formula (II), R5 and R6, together with the nitrogen to which they are attached, form a spirocycle wherein the spirocycle is substituted with one or more substituents independently selected from optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocycle, optionally substituted cycloalkyl, haloalkyl, —OR7, —NR12R13, —C(O)OR9, —C(O)NR9R10, —NR8SO2R9, —NR8C(O)OR9, and —NR8C(O)NR9R10; and the spirocycle optionally contains an additional O, N, or S. In some embodiments of a compound of Formula (II), R5 and R6, together with the nitrogen to which they are attached, form a spirocycle selected from




wherein R17 is optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocycle, optionally substituted cycloalkyl, haloalkyl, —C(O)OR9, or —C(O)NR9R10. In further embodiments, R17 is aryl, heteroaryl, heterocycle, cycloalkyl, haloalkyl, —C(O)OR9, or —C(O)NR9R10. In still further embodiments, R17 is cycloalkyl, haloalkyl, —C(O)OR9, or —C(O)NR9R10.

In some embodiments of a compound of Formula (II), R5 and R6, together with the nitrogen to which they are attached, form a 7-8 membered bridged heterocyclic ring optionally containing an additional O, N, or S, and optionally substituted with one or more substituents independently selected from halogen, —CN, oxo, optionally substituted C1-6 alkyl, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocycle, optionally substituted cycloalkyl, —S(O)wR11, —OR3, —OR7, —C(O)R9, —C(O)OR9, —C(O)NR9R10, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, and —NR8C(O)NR9R10. In some embodiments of a compound of Formula (II), R5 and R6, together with the nitrogen to which they are attached, form a 7-8 membered bridged heterocyclic ring containing an additional O, N, or S. In some embodiments of a compound of Formula (II), R5 and R6, together with the nitrogen to which they are attached, form an 8-membered bridged heterocyclic ring selected from and




In some embodiments of a compound of Formula (II), R1 is halogen, —CH3, —CF3, —OCH3, or —OCF3. In some embodiments of a compound of Formula (II), R1 is halogen. In some embodiments of a compound of Formula (II), R1 is —Cl. In some embodiments of a compound of Formula (II), R1 is —CH3. In some embodiments of a compound of Formula (II), R1 is —CF3. In some embodiments of a compound of Formula (II), R1 is —OCH3. In some embodiments of a compound of Formula (II), R1 is —OCF3.
Another embodiment provides a compound of Formula (IIa):




wherein:

    R1 is halogen, —OR3, —CN, aryl (optionally substituted by one, two, or three moieties each independently selected from R4), aryloxy (optionally substituted by one, two, or three moieties each independently selected from R4), heteroaryl (optionally substituted by one, two, or three moieties each independently selected from R4), heteroaryloxy (optionally substituted by one, two, or three moieties each independently selected from R4), heterocycle (optionally substituted by one, two, or three moieties each independently selected from R4), cycloalkyl (optionally substituted by one, two, or three moieties each independently selected from R4), optionally substituted C1-6 alkyl, optionally substituted C2-6 alkenyl, optionally substituted C2-6 alkynyl, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, —NR8C(O)NR9R10, —C(O)R9, —C(O)OR9, —C(O)NR9R10, —S(O)wR11, or —SH;
    each R3 is independently selected from H, C1-6 alkyl, haloalkyl, and aminoalkyl;
    each R4 is independently selected from halogen, —OR3, —CN, nitro, optionally substituted C1-6 alkyl, optionally substituted C1-6 alkenyl, optionally substituted C1-6 alkynyl, cycloalkyl, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, —NR8C(O)NR9R10, —C(O)R9, —C(O)OR9, —C(O)NR9R10, —S(O)wR11, and —SH;
    R5 and R6, together with the nitrogen to which they are attached, form a monocyclic heterocycle, a fused bicyclic heterocycle, or a spirocycle; wherein the monocyclic heterocycle, the fused bicyclic heterocycle, or the spirocycle is substituted with hydroxyalkyl; and the monocyclic heterocycle, the fused bicyclic heterocycle, or the spirocycle optionally contains an additional O, N, or S;
    each R8 is independently selected from H, optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl;
    each R9 and R10 is independently selected from H, optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl; or R9 and R10 together with the nitrogen to which they are attached form an optionally substituted heterocycle optionally containing an additional O, N, or S;
    each R11 is independently selected from optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl; and
    w is 0, 1, or 2;

or a solvate, hydrate, tautomer, N-oxide, or pharmaceutically acceptable salt thereof.



In some embodiments of a compound of Formula (IIa), R5 and R6, together with the nitrogen to which they are attached, form a monocyclic heterocycle wherein the monocyclic heterocycle is substituted with hydroxyalkyl, and the monocyclic heterocycle optionally contains an additional O, N, or S. In some embodiments of a compound of Formula (IIa), R5 and R6 together with the nitrogen to which they are attached, form a monocyclic heterocycle wherein the monocyclic heterocycle is substituted with hydroxymethyl, and the monocyclic heterocycle optionally contains an additional O, N, or S. In some embodiments of a compound of Formula (IIa), R5 and R6, together with the nitrogen to which they are attached, form a piperidine, pyrrolidine, morpholine, or azetidine wherein the piperidine, pyrrolidine, morpholine, or azetidine is substituted with hydroxyalkyl. In some embodiments of a compound of Formula (IIa), R5 and R6, together with the nitrogen to which they are attached, form a piperidine, pyrrolidine, morpholine, or azetidine wherein the piperidine, pyrrolidine, morpholine, or azetidine is substituted with hydroxymethyl. In some embodiments of a compound of Formula (IIa), R5 and R6, together with the nitrogen to which they are attached, form a piperidine wherein the piperidine is substituted with hydroxyalkyl. In some embodiments of a compound of Formula (IIa), R5 and R6, together with the nitrogen to which they are attached, form a piperidine wherein the piperidine is substituted with hydroxymethyl. In some embodiments of a compound of Formula (IIa), R5 and R6, together with the nitrogen to which they are attached, form a pyrrolidine wherein the pyrrolidine is substituted with hydroxyalkyl. In some embodiments of a compound of Formula (IIa), R5 and R6, together with the nitrogen to which they are attached, form a pyrrolidine wherein the pyrrolidine is substituted with hydroxymethyl. In some embodiments of a compound of Formula (IIa), R5 and R6, together with the nitrogen to which they are attached, form a morpholine wherein the morpholine is substituted with hydroxyalkyl. In some embodiments of a compound of Formula (IIa), R5 and R6, together with the nitrogen to which they are attached, form a morpholine wherein the morpholine is substituted with hydroxymethyl. In some embodiments of a compound of Formula (IIa), R5 and R6, together with the nitrogen to which they are attached, form an azetidine wherein the azetidine is substituted with hydroxyalkyl. In some embodiments of a compound of Formula (IIa), R5 and R6, together with the nitrogen to which they are attached, form an azetidine wherein the azetidine is substituted with hydroxymethyl.
In some embodiments of a compound of Formula (IIa), R5 and R6, together with the nitrogen to which they are attached, form a fused bicyclic heterocycle wherein the fused bicyclic heterocycle is substituted with hydroxyalkyl, and the fused bicyclic heterocycle optionally contains an additional O, N, or S. In some embodiments of a compound of Formula (IIa), R5 and R6, together with the nitrogen to which they are attached, form a fused bicyclic heterocycle wherein the fused bicyclic heterocycle is substituted with hydroxymethyl, and the fused bicyclic heterocycle optionally contains an additional O, N, or S. In some embodiments of a compound of Formula (IIa), R5 and R6, together with the nitrogen to which they are attached, form




wherein R17 is hydroxyalkyl.

In some embodiments of a compound of Formula (IIa), R5 and R6, together with the nitrogen to which they are attached, form a spirocycle wherein the spirocycle is substituted with hydroxyalkyl, and the spirocycle optionally contains an additional O, N, or S. In some embodiments of a compound of Formula (IIa), R5 and R6, together with the nitrogen to which they are attached, form a spirocycle wherein the spirocycle is substituted with hydroxymethyl, and the spirocycle optionally contains an additional O, N, or S. In some embodiments of a compound of Formula (IIa), R5 and R6, together with the nitrogen to which they are attached, form a spirocycle selected from




wherein R17 is hydroxyalkyl.

In some embodiments of a compound of Formula (IIa), R1 is halogen, —CH3, —CF3, —OCH3, or —OCF3. In some embodiments of a compound of Formula (IIa), R1 is halogen. In some embodiments of a compound of Formula (IIa), R1 is —Cl. In some embodiments of a compound of Formula (IIa), R1 is —CH3. In some embodiments of a compound of Formula (IIa), R1 is —CF3. In some embodiments of a compound of Formula (IIa), R1 is —OCH3. In some embodiments of a compound of Formula (IIa), R1 is —OCF3.
Another embodiment provides a compound of Formula (III):




wherein:

    R1 is halogen, —OR3, —CN, aryl (optionally substituted by one, two, or three moieties each independently selected from R4), aryloxy (optionally substituted by one, two, or three moieties each independently selected from R4), heteroaryl (optionally substituted by one, two, or three moieties each independently selected from R4), heteroaryloxy (optionally substituted by one, two, or three moieties each independently selected from R4), heterocycle (optionally substituted by one, two, or three moieties each independently selected from R4), cycloalkyl (optionally substituted by one, two, or three moieties each independently selected from R4), optionally substituted C1-6 alkyl, optionally substituted C2-6 alkenyl, optionally substituted C2-6 alkynyl, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, —NR8C(O)NR9R10, —C(O)R9, —C(O)OR9, —C(O)NR9R10, —S(O)wR11, or —SH;
    each R3 is independently selected from H, C1-6 alkyl, haloalkyl, and aminoalkyl;
    each R4 is independently selected from halogen, —OR3, —CN, nitro, optionally substituted C1-6 alkyl, optionally substituted C1-6 alkenyl, optionally substituted C1-6 alkynyl, cycloalkyl, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, —NR8C(O)NR9R10, —C(O)R9, —C(O)OR9, —C(O)NR9R10, —S(O)wR11, and —SH;
    each R8 is independently selected from H, optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl;
    each R9 and R10 is independently selected from H, optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl; or R9 and R10 together with the nitrogen to which they are attached form an optionally substituted heterocycle optionally containing an additional O, N, or S;
    each R11 is independently selected from optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl;
    R14 and R15 together with the nitrogen to which they are attached form an optionally substituted heterocycle optionally containing an additional O, N, or S;
    w is 0, 1, or 2; and
    x is 1, 2, or 3;

or a solvate, hydrate, tautomer, N-oxide, stereoisomer, or pharmaceutically acceptable salt thereof.



In some embodiments of a compound of Formula (III), R14 and R15, together with the nitrogen to which they are attached, form a heterocycle optionally containing an additional O, N, or S; wherein the heterocycle is substituted with one or more substituents independently selected from halogen, oxo, —OR3, —CN, aryl (optionally substituted by one, two, or three moieties each independently selected from R4), aryloxy (optionally substituted by one, two, or three moieties each independently selected from R4), heteroaryl (optionally substituted by one, two, or three moieties each independently selected from R4), heteroaryloxy (optionally substituted by one, two, or three moieties each independently selected from R4), heterocycle (optionally substituted by one, two, or three moieties each independently selected from R4), cycloalkyl (optionally substituted by one, two, or three moieties each independently selected from R4), optionally substituted C1-6 alkyl, optionally substituted C2-6 alkenyl, optionally substituted C2-6 alkynyl, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, —S(O)wR11, —NR8C(O)NR9R10, —C(O)R9, —C(O)OR9, and —C(O)NR9R10. In some embodiments of a compound of Formula (III), R14 and R15, together with the nitrogen to which they are attached, form a substituted heterocycle; wherein the substituted heterocycle has one or more substituents independently selected from halogen, oxo, —OR3, —CN, C1-6 alkyl, haloalkyl, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, —S(O)wR11, —NR8C(O)NR9R10, —C(O)R9, —C(O)OR9, and —C(O)NR9R10. In some embodiments of a compound of Formula (III), R14 and R15, together with the nitrogen to which they are attached, form an optionally substituted pyrrolidine, optionally substituted piperidine, optionally substituted morpholine, or optionally substituted piperazine. In some embodiments of a compound of Formula (III), R14 and R15, together with the nitrogen to which they are attached, form a substituted or unsubstituted pyrrolidine. In some embodiments of a compound of Formula (III), R14 and R15, together with the nitrogen to which they are attached, form a substituted or unsubstituted piperidine. In some embodiments of a compound of Formula (III), R14 and R15, together with the nitrogen to which they are attached, form a substituted or unsubstituted morpholine. In some embodiments of a compound of Formula (III), R14 and R15, together with the nitrogen to which they are attached, form a substituted or unsubstituted piperazine. In some embodiments of a compound of Formula (III), R14 and R15, together with the nitrogen to which they are attached, form an optionally substituted heterocycle selected from:




In some embodiments of a compound of Formula (III), R1 is halogen, —CH3, —CF3, —OCH3, or —OCF3. In some embodiments of a compound of Formula (III), R1 is halogen. In some embodiments of a compound of Formula (III), R1 is —Cl. In some embodiments of a compound of Formula (III), R1 is —CH3. In some embodiments of a compound of Formula (III), R1 is —CF3. In some embodiments of a compound of Formula (III), R1 is —OCH3. In some embodiments of a compound of Formula (III), R1 is —OCF3.
Another embodiment provides a compound of Formula (IV):




wherein:

    A and B are independently a bond or CR18R19;
    C is O or CR18R19OCR20R21;
    D is CR18R19 or CR18R19CR20R21;
    R1 is halogen, —OR3, —CN, aryl (optionally substituted by one, two, or three moieties each independently selected from R4), aryloxy (optionally substituted by one, two, or three moieties each independently selected from R4), heteroaryl (optionally substituted by one, two, or three moieties each independently selected from R4), heteroaryloxy (optionally substituted by one, two, or three moieties each independently selected from R4), heterocycle (optionally substituted by one, two, or three moieties each independently selected from R4), cycloalkyl (optionally substituted by one, two, or three moieties each independently selected from R4), optionally substituted C1-6 alkyl, optionally substituted C2-6 alkenyl, optionally substituted C2-6 alkynyl, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, —NR8C(O)NR9R10, —C(O)R9, —C(O)OR9, —C(O)NR9R10, —S(O)wR11, or —SH;
    each R3 is independently selected from H, C1-6 alkyl, haloalkyl, and aminoalkyl;
    each R4 is independently selected from halogen, —OR3, —CN, nitro, optionally substituted C1-6 alkyl, optionally substituted C1-6 alkenyl, optionally substituted C1-6 alkynyl, cycloalkyl, —NR9R10, —NR8C(O)R9, —NR8SO2R9, —NR8C(O)OR9, —NR8C(O)NR9R10, —C(O)R9, —C(O)OR9, —C(O)NR9R10, —S(O)wR11, and —SH;
    each R8 is independently selected from H, optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl;
    each R9 and R10 is independently selected from H, optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl; or R9 and R10 together with the nitrogen to which they are attached form an optionally substituted heterocycle optionally containing an additional O, N, or S;
    each R11 is independently selected from optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted heterocycloalkyl;
    R17 is H, optionally substituted C1-6 alkyl, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocycle, optionally substituted cycloalkyl, —S(O)wR11, —C(O)R9, —C(O)OR9, or —C(O)NR9R10;
    each R18, R19, R20, and R21 is independently selected from H, optionally substituted C1-6 alkyl, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocycle, optionally substituted cycloalkyl, —C(O)R9, —C(O)OR9, and —C(O)NR9R10; and
    w is 0, 1, or 2;

or a solvate, hydrate, tautomer, N-oxide, stereoisomer, or pharmaceutically acceptable salt thereof.



In some embodiments of a compound of Formula (IV), R18, R19, R20, and R21 are H.
In some embodiments of a compound of Formula (IV), A and B are both bonds; D is CR18R19CR20R21; and C is CR18R19OCR20R21. In some embodiments of a compound of Formula (IV), A and B are both bonds; D is CH2CH2; and C is CH2OCH2.
In some embodiments of a compound of Formula (IV), A and B are both CR18R19; D is CR18R19CR20R21; and C is O. In some embodiments of a compound of Formula (IV), A and B are both CH2; D is CH2CH2; and C is O.
In some embodiments of a compound of Formula (IV), R1 is halogen, —CH3, —CF3, —OCH3, or —OCF3. In some embodiments of a compound of Formula (IV), R1 is halogen. In some embodiments of a compound of Formula (IV), R1 is —Cl. In some embodiments of a compound of Formula (IV), R1 is —CH3. In some embodiments of a compound of Formula (IV), R1 is —CF3. In some embodiments of a compound of Formula (IV), R1 is —OCH3. In some embodiments of a compound of Formula (IV), R1 is —OCF3.
Further embodiments provided herein include combinations of one or more of the particular embodiments set forth above.
In some embodiments, the compound disclosed herein has the structure provided in Table 1.









TABLE 1





Chemical




Synthesis




Example
Structure
Name







 1




1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(2- (3-(prop-2-yn-1-yloxy)pyrrolidin-1-yl)-6- (trifluoromethyl)benzyl)piperazine-1- carboxylate


 


 2




1,1,1,3,3,3-hexafluoropropan-2-yl (R)-4-(2- (3-(prop-2-yn-1-yloxy)pyrrolidin-1-yl)-4- (trifluoromethyl)benzyl)piperazine-1- carboxylate


 


 3




1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(4- chloro-2-(3-(prop-2-yn-1-yloxy)pyrrolidin- 1-yl)benzyl)piperazine-1-carboxylate


 


 4




1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(4- chloro-2-(3-(fluoromethyl)pyrrolidin-1- yl)benzyl)piperazine-1-carboxylate


 


 5




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3- (methoxycarbonyl)-4- morpholinobenzyl)piperazine-1- carboxylate


 


 6




1,1,1,3,3,3-hexafluoropropan-2-yl 4-((3- (prop-2-yn-1-yloxy)-[1,1′-biphenyl]-4- yl)methyl)piperazine-1-carboxylate


 


 7




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-((4- acetylpiperazin-1-yl)methyl)-2- methoxybenzyl)piperazine-1-carboxylate


 


 8




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2- methyl-3-((4-(methylsulfonyl)piperazin-1- yl)methyl)benzyl)piperazine-1-carboxylate


 


 9




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-((4- fluoropiperidin-1-yl)methyl)-2- methoxybenzyl)piperazine-1-carboxylate


 


10




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2- methyl-3-((4- (methylsulfonamido)piperidin-1- yl)methyl)benzyl)piperazine-1-carboxylate


 


11




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3((4- acetamidopiperidin-1-yl)methyl)-2- methylbenzyl)piperazine-1-carboxylate


 


12




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3- ((4,4-difluoropiperidin-1-yl)methyl)-2- methylbenzyl)piperazine-1-carboxylate


 


13




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2- methyl-3-(pyrrolidin-1- ylmethyl)benzyl)piperazine-1-carboxylate


 


14




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-((4- acetylpiperazin-1-yl)methyl)-2- methylbenzyl)piperazine-1-carboxylate


 


15




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-((4- fluoropiperidin-1-yl)methyl)-2- methylbenzyl)piperazine-1-carboxylate


 


16




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3- ((4,4-difluoropiperidin-1-yl)methyl)-2- methoxybenzyl)piperazine-1-carboxylate


 


17




1,1,1,3,3,3-hexafluoropropan-2-yl (R)-4-(3- ((3-acetamidopyrrolidin-1-yl)methyl)-2- methylbenzyl)piperazine-1-carboxylate


 


18




1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(3- ((3-acetamidopyrrolidin-1-yl)methyl)-2- methylbenzyl)piperazine-1-carboxylate


 


19




1,1,1,3,3,3-hexafluoropropan-2-yl (R)-4-(2- methyl-3-((2-methylpiperidin-1- yl)methyl)benzyl)piperazine-1-carboxylate


 


20




1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(2- methyl-3-((2-methylpiperidin-1- yl)methyl)benzyl)piperazine-1-carboxylate


 


21




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2- methyl-3-(morpholinomethyl)benzyl) piperazine-1-carboxylate


 


22




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2- fluoro-3-(morpholinomethyl)benzyl) piperazine-1-carboxylate


 


23




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(4- (morpholinomethyl)-2- (trifluoromethoxy)benzyl)piperazine-1- carboxylate


 


24




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(4- (piperidin-1-ylmethyl)-2- (trifluoromethoxy)benzyl)piperazine-1- carboxylate


 


25




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(4- (pyrrolidin-1-ylmethyl)-2- (trifluoromethoxy)benzyl)piperazine-1- carboxylate


 


26




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-(8- oxa-3-azabicyclo[3.2.1]octan-3-yl)-4- (trifluoromethyl)benzyl)piperazine-1- carboxylate


 


27




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(4-(8- oxa-3-azabicyclo[3.2.1]octan-3-yl)-2- fluorobenzyl)piperazine-1-carboxylate


 


28




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(4-(8- oxa-3-azabicyclo[3.2.1]octan-3-yl)-2- (trifluoromethoxy)benzyl)piperazine-1- carboxylate


 


29




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3- chloro-5-(pyrrolidin-1- ylmethyl)benzyl)piperazine-1-carboxylate


 


30




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(8- oxa-3-azabicyclo[3.2.1]octan-3-yl)-4- chlorobenzyl)piperazine-1-carboxylate


 


31




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(8- oxa-3-azabicyclo[3.2.1]octan-3-yl)-3- (trifluoromethoxy)benzyl)piperazine-1- carboxylate


 


32




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(4- (pyrrolidin-1-yl)piperidin-1-yl)-3- (trifluoromethoxy)benzyl)piperazine-1- carboxylate


 


33




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(4- carbamoylpiperidin-1-yl)-4- chlorobenzyl)piperazine-1-carboxylate


 


34




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(4- chloro-2-(4-(dimethylcarbamoyl)piperidin- 1-yl)benzyl)piperazine-1-carboxylate


 


35




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(4- (dimethylcarbamoyl)piperidin-1-yl)-4- (trifluoromethyl)benzyl)piperazine-1- carboxylate


 


36




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(4- carbamoylpiperidin-1-yl)-4- (trifluoromethyl)benzyl)piperazine-1- carboxylate


 


37




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(4- chloro-2-(4-(pyrrolidine-1- carbonyl)piperidin-1-yl)benzyl)piperazine- 1-carboxylate


 


38




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(4- chloro-2-(3-(3,5-dimethyl-1H-pyrazol-1- yl)azetidin-1-yl)benzyl)piperazine-1- carboxylate


 


39




1,1,1,3,3,3-hexafluoropropan-2-yl (R)-4-(4- chloro-2-(3- (methylsulfonamido)pyrrolidin-1- yl)benzyl)piperazine-1-carboxylate


 


40




1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(4- chloro-2-(3- (methylsulfonamido)pyrrolidin-1- yl)benzyl)piperazine-1-carboxylate


 


41




1,1,1,3,3,3-hexafluoropropan-2-yl (R)-4-(4- chloro-2-(2-oxo-[1,3′-bipyrrolidin]-1′- yl)benzyl)piperazine-1-carboxylate


 


42




1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(4- chloro-2-(2-oxo-[1,3′-bipyrrolidin]-1′- yl)benzyl)piperazine-1-carboxylate


 


43




1,1,1,3,3,3-hexafluoropropan-2-yl (R)-4-(4- chloro-2-(3-(2-oxopiperidin-1- yl)pyrrolidin-1-yl)benzyl)piperazine-1- carboxylate


 


44




1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(4- chloro-2-(3-(2-oxopiperidin-1- yl)pyrrolidin-1-yl)benzyl)piperazine-1- carboxylate


 


45




1,1,1,3,3,3-hexafluoropropan-2-yl (R)-4-(4- chloro-2-(3-(1,1-dioxidoisothiazolidin-2- yl)pyrrolidin-1-yl)benzyl)piperazine-1- carboxylate


 


46




1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(4- chloro-2-(3-(1,1-dioxidoisothiazolidin-2- yl)pyrrolidin-1-yl)benzyl)piperazine-1- carboxylate


 


47




1,1,1,3,3,3-hexafluoropropan-2-yl (R)-4-(2- (3-(methylsulfonamido)pyrrolidin-1-yl)-6- (trifluoromethyl)benzyl)piperazine-1- carboxylate


 


48




1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(2- (3-(methylsulfonamido)pyrrolidin-1-yl)-6- (trifluoromethyl)benzyl)piperazine-1- carboxylate


 


49




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(3- oxa-8-azabicyclo[3.2.1]octan-8-yl)-6- (trifluoromethyl)benzyl)piperazine-1- carboxylate


 


50




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(8- oxa-3-azabicyclo[3.2.1]octan-3-yl)-6- (trifluoromethyl)benzyl)piperazine-1- carboxylate


 


51




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3- methyl-2-(4-(methylsulfonamido)piperidin- 1-yl)benzyl)piperazine-1-carboxylate


 


52




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2- methyl-3-(4-(methylsulfonamido)piperidin- 1-yl)benzyl)piperazine-1-carboxylate


 


53




1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(2- (3-(fluoromethyl)pyrrolidin-1-yl)-4- methylbenzyl)piperazine-1-carboxylate


 


54




1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(2- (3-(fluoromethyl)pyrrolidin-1-yl)-4- (trifluoromethyl)benzyl)piperazine-1- carboxylate


 


55




1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(3- chloro-2-(3-(fluoromethyl)pyrrolidin-1- yl)benzyl)piperazine-1-carboxylate


 


56




1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(2- (3-(fluoromethyl)pyrrolidin-1-yl)-3- methylbenzyl)piperazine-1-carboxylate


 


57




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(8- oxa-3-azabicyclo[3.2.1]octan-3-yl)-3- chlorobenzyl)piperazine-1-carboxylate


 


58




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(3- oxa-8-azabicyclo[3.2.1]octan-8-yl)-4- (trifluoromethyl)benzyl)piperazine-1- carboxylate


 


59




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(8- oxa-3-azabicyclo[3.2.1]octan-3-yl)-4- (trifluoromethyl)benzyl)piperazine-1- carboxylate


 


60




1,1,1,3,3,3-hexafluoropropan-2-yl (R)-4-(2- (3-(methylsulfonamido)pyrrolidin-1-yl)-4- (trifluoromethyl)benzyl)piperazine-1- carboxylate


 


61




1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(2- (3-(methylsulfonamido)pyrrolidin-1-yl)-4- (trifluoromethyl)benzyl)piperazine-1- carboxylate


 


62




1-(5-chloro-2-((4-(((1,1,1,3,3,3- hexafluoropropan-2- yl)oxy)carbonyl)piperazin-1- yl)methyl)phenyl)piperidine-4-carboxylic acid


 


63




1,1,1,3,3,3-hexafluoropropan-2- yl)oxy)carbonyl)piperazin-1-yl)methyl)-5- (trifluoromethyl)phenyl)piperidine-4- carboxylic acid


 


64




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(4- chloro-2-(5- cyclopropylhexahydropyrrolo[3,4-c]pyrrol- 2(1H)-yl)benzyl)piperazine-1-carboxylate


 


65




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(5- cyclopropylhexahydropyrrolo[3,4-c]pyrrol- 2(1H)-yl)-4- (trifluoromethyl)benzyl)piperazine-1- carboxylate


 


66




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(4- chloro-2-(1-cyclopropyl-1,8- diazaspiro[4.5]decan-8- yl)benzyl)piperazine-1-carboxylate


 


67




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(1- cyclopropyl-1,8-diazaspiro[4.5]decan-8- yl)-4-(trifluoromethyl)benzyl)piperazine-1- carboxylate


 


68




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(4- (pyrrolidine-1-carbonyl)piperidin-1-yl)-4- (trifluoromethyl)benzyl)piperazine-1- carboxylate


 


69




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(4- (pyrrolidine-1-carbonyl)piperidin-1-yl)-4- (trifluoromethyl)benzyl)piperazine-1- carboxylate


 


70




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(2- (ethoxycarbonyl)pyrrolidin-1-yl)-4- (trifluoromethyl)benzyl)piperazine-1- carboxylate


 


71




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(2- (hydroxymethyl)pyrrolidin-1-yl)-4- (trifluoromethyl)benzyl)piperazine-1- carboxylate


 


72




1-(2-((4-(((1,1,1,3,3,3-hexafluoropropan-2- yl)oxy)carbonyl)piperazin-1-yl)methyl)-5- (trifluoromethyl)phenyl)pyrrolidine-3- carboxylic acid


 


73




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(3- (hydroxymethyl)pyrrolidin-1-yl)-4- (trifluoromethyl)benzyl)piperazine-1- carboxylate


 


74




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(4- (hydroxymethyl)piperidin-1-yl)-4- (trifluoromethyl)benzyl)piperazine-1- carboxylate


 


75




4-(2-((4-(((1,1,1,3,3,3-hexafluoropropan-2- yl)oxy)carbonyl)piperazin-1-yl)methyl)-5- (trifluoromethyl)phenyl)morpholine-2- carboxylic acid


 


76




2-(2-((4-(((1,1,1,3,3,3-hexafluoropropan-2- yl)oxy)carbonyl)piperazin-1-yl)methyl)-5- (trifluoromethyl)phenyl)-2-oxoacetic acid


 


77




(5-((4-(((1,1,1,3,3,3-hexafluoropropan-2- yl)oxy)carbonyl)piperazin-1-yl)methyl)-2- (trifluoromethyl)phenyl)proline


 


78




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-(2- (ethoxycarbonyl)pyrrolidin-1-yl)-4- (trifluoromethyl)benzyl)piperazine-1- carboxylate


 


79




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-(2- (hydroxymethyl)pyrrolidin-1-yl)-4- (trifluoromethyl)benzyl)piperazine-1- carboxylate


 


80




1-(5-((4-(((1,1,1,3,3,3-hexafluoropropan-2- yl)oxy)carbonyl)piperazin-1-yl)methyl)-2- (trifluoromethyl)phenyl)pyrrolidine-3- carboxylic acid


 


81




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-(3- (hydroxymethyl)pyrrolidin-1-yl)-4- (trifluoromethyl)benzyl)piperazine-1- carboxylate


 


82




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-(4- (hydroxymethyl)piperidin-1-yl)-4- (trifluoromethyl)benzyl)piperazine-1- carboxylate


 


83




4-(5-((4-(((1,1,1,3,3,3-hexafluoropropan-2- yl)oxy)carbonyl)piperazin-1-yl)methyl)-2- (trifluoromethyl)phenyl)morpholine-2- carboxylic acid


 


84




2-(5-((4-(((1,1,1,3,3,3-hexafluoropropan-2- yl)oxy)carbonyl)piperazin-1-yl)methyl)-2- (trifluoromethyl)phenyl)-2-oxoacetic acid


 


85




1-(5-((4-(((1,1,1,3,3,3-hexafluoropropan-2- yl)oxy)carbonyl)piperazin-1-yl)methyl)-2- (trifluoromethyl)phenyl)piperidine-4- carboxylic acid


 


86




(3-((4-(((1,1,1,3,3,3-hexafluoropropan-2- yl)oxy)carbonyl)piperazin-1-yl)methyl)-5- (trifluoromethyl)phenyl)proline


 


87




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-(2- (ethoxycarbonyl)pyrrolidin-1-yl)-5- (trifluoromethyl)benzyl)piperazine-1- carboxylate


 


88




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-(2- (hydroxymethyl)pyrrolidin-1-yl)-5- (trifluoromethyl)benzyl)piperazine-1- carboxylate


 


89




1-(3-((4-(((1,1,1,3,3,3- hexafluoropropan-2- yl)oxy)carbonyl)piperazin-1- yl)methyl)-5- (trifluoromethyl)phenyl)pyrrolidine- 3-carboxylic acid


 


90




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-(3- (hydroxymethyl)pyrrolidin-1-yl)-5- (trifluoromethyl)benzyl)piperazine-1- carboxylate


 


91




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-(4- (hydroxymethyl)piperidin-1-yl)-5- (trifluoromethyl)benzyl)piperazine-1- carboxylate


 


92




4-(3-((4-(((1,1,1,3,3,3-hexafluoropropan-2- yl)oxy)carbonyl)piperazin-1-yl)methyl)-5- (trifluoromethyl)phenyl)morpholine-2- carboxylic acid


 


93




2-(3-((4-(((1,1,1,3,3,3-hexafluoropropan-2- yl)oxy)carbonyl)piperazin-1-yl)methyl)-5- (trifluoromethyl)phenyl)-2-oxoacetic acid


 


94




1-(3-((4-(((1,1,1,3,3,3-hexafluoropropan-2- yl)oxy)carbonyl)piperazin-1-yl)methyl)-5- (trifluoromethyl)phenyl)piperidine-4- carboxylic acid


 


95




4-(2-((4-(((1,1,1,3,3,3-hexafluoropropan-2- yl)oxy)carbonyl)piperazin-1-yl)methyl)-5- (trifluoromethyl)phenyl)morpholine-3- carboxylic acid









In some embodiments, the compound disclosed herein has the structure provided in Table 1A.









TABLE 1A





Chemical




Synthesis




Example
Structure
Name







 96




1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(4- (1H-tetrazol-5-yl)piperidin-1-yl)-4- (trifluoromethyl)benzyl)piperazine-1- carboxylate


 


 97




4-(3-((4-(((1,1,1,3,3,3-hexafluoropropan-2- yl)oxy)carbonyl)piperazin-1-yl)methyl)-5- (trifluoromethyl)phenyl)morpholine-3- carboxylic acid


 


 98




(R)-1-(2-((4-(((1,1,1,3,3,3- hexafluoropropan-2- yl)oxy)carbonyl)piperazin-1-yl)methyl)-5- (trifluoromethyl)phenyl)piperidine-3- carboxylic acid


 


 99




(S)-1-(2-((4-(((1,1,1,3,3,3- hexafluoropropan-2- yl)oxy)carbonyl)piperazin-1-yl)methyl)-5- (trifluoromethyl)phenyl)piperidine-3- carboxylic acid


 


100




(R)-1-(3-((4-(((1,1,1,3,3,3- hexafluoropropan-2- yl)oxy)carbonyl)piperazin-1-yl)methyl)-5- (trifluoromethyl)phenyl)piperidine-3- carboxylic acid


 


101




(S)-1-(3-((4-(((1,1,1,3,3,3- hexafluoropropan-2- yl)oxy)carbonyl)piperazin-1-yl)methyl)-5- (trifluoromethyl)phenyl)piperidine-3- carboxylic acid









Preparation of the Compounds

The compounds used in the reactions described herein are made according to known organic synthesis techniques, starting from commercially available chemicals and/or from compounds described in the chemical literature. “Commercially available chemicals” are obtained from standard commercial sources including Acros Organics (Geel, Belgium), Aldrich Chemical (Milwaukee, Wis., including Sigma Chemical and Fluka), Apin Chemicals Ltd. (Milton Park, UK), Ark Pharm, Inc. (Libertyville, Ill.), Avocado Research (Lancashire, U.K.), BDH Inc. (Toronto, Canada), Bionet (Cornwall, U.K.), Chemservice Inc. (West Chester, Pa.), Combi-blocks (San Diego, Calif.), Crescent Chemical Co. (Hauppauge, N.Y.), eMolecules (San Diego, Calif.), Fisher Scientific Co. (Pittsburgh, Pa.), Fisons Chemicals (Leicestershire, UK), Frontier Scientific (Logan, Utah), ICN Biomedicals, Inc. (Costa Mesa, Calif.), Key Organics (Cornwall, U.K.), Lancaster Synthesis (Windham, N.H.), Matrix Scientific, (Columbia, S.C.), Maybridge Chemical Co. Ltd. (Cornwall, U.K.), Parish Chemical Co. (Orem, Utah), Pfaltz & Bauer, Inc. (Waterbury, Conn.), Polyorganix (Houston, Tex.), Pierce Chemical Co. (Rockford, Ill.), Riedel de Haen AG (Hanover, Germany), Ryan Scientific, Inc. (Mount Pleasant, S.C.), Spectrum Chemicals (Gardena, Calif.), Sundia Meditech, (Shanghai, China), TCI America (Portland, Oreg.), Trans World Chemicals, Inc. (Rockville, Md.), and WuXi (Shanghai, China).
Suitable reference books and treatises that detail the synthesis of reactants useful in the preparation of compounds described herein, or provide references to articles that describe the preparation, include for example, “Synthetic Organic Chemistry”, John Wiley & Sons, Inc., New York; S. R. Sandler et al., “Organic Functional Group Preparations,” 2nd Ed., Academic Press, New York, 1983; H. O. House, “Modern Synthetic Reactions”, 2nd Ed., W. A. Benjamin, Inc. Menlo Park, Calif. 1972; T. L. Gilchrist, “Heterocyclic Chemistry”, 2nd Ed., John Wiley & Sons, New York, 1992; J. March, “Advanced Organic Chemistry: Reactions, Mechanisms and Structure”, 4th Ed., Wiley-Interscience, New York, 1992. Additional suitable reference books and treatises that detail the synthesis of reactants useful in the preparation of compounds described herein, or provide references to articles that describe the preparation, include for example, Fuhrhop, J. and Penzlin G. “Organic Synthesis: Concepts, Methods, Starting Materials”, Second, Revised and Enlarged Edition (1994) John Wiley & Sons ISBN: 3-527-29074-5; Hoffman, R. V. “Organic Chemistry, An Intermediate Text” (1996) Oxford University Press, ISBN 0-19-509618-5; Larock, R. C. “Comprehensive Organic Transformations: A Guide to Functional Group Preparations” 2nd Edition (1999) Wiley-VCH, ISBN: 0-471-19031-4; March, J. “Advanced Organic Chemistry: Reactions, Mechanisms, and Structure” 4th Edition (1992) John Wiley & Sons, ISBN: 0-471-60180-2; Otera, J. (editor) “Modern Carbonyl Chemistry” (2000) Wiley-VCH, ISBN: 3-527-29871-1; Patai, S. “Patai's 1992 Guide to the Chemistry of Functional Groups” (1992) Interscience ISBN: 0-471-93022-9; Solomons, T. W. G. “Organic Chemistry” 7th Edition (2000) John Wiley & Sons, ISBN: 0-471-19095-0; Stowell, J. C., “Intermediate Organic Chemistry” 2nd Edition (1993) Wiley-Interscience, ISBN: 0-471-57456-2; “Industrial Organic Chemicals: Starting Materials and Intermediates: An Ullmann's Encyclopedia” (1999) John Wiley & Sons, ISBN: 3-527-29645-X, in 8 volumes; “Organic Reactions” (1942-2000) John Wiley & Sons, in over 55 volumes; and “Chemistry of Functional Groups” John Wiley & Sons, in 73 volumes.
Specific and analogous reactants are also identified through the indices of known chemicals prepared by the Chemical Abstract Service of the American Chemical Society, which are available in most public and university libraries, as well as through on-line databases (the American Chemical Society, Washington, D.C., may be contacted for more details). Chemicals that are known but not commercially available in catalogs are optionally prepared by custom chemical synthesis houses, where many of the standard chemical supply houses (e.g., those listed above) provide custom synthesis services. A reference for the preparation and selection of pharmaceutical salts of the piperazine carbamates described herein is P. H. Stahl & C. G. Wermuth “Handbook of Pharmaceutical Salts”, Verlag Helvetica Chimica Acta, Zurich, 2002.
The piperazine carbamates are prepared by the general synthetic routes described below in Schemes 1-3.




A method for preparing compounds of formula D is provided in Scheme 1. Reductive amination of an aldehyde of formula A with Boc-protected piperazine, using an agent such as sodium triacetoxyborohydride, affords intermediate B. Following Boc-group removal with an acid such as trifluoroacetic acid or HCl, intermediate C is coupled with hexafluoropropan-2-yl chloroformate to provide a piperazine carbamate of formula D. In some embodiments, intermediate B undergoes one or more additional reactions to alter R1 and/or R2 prior to Boc-group removal.




Another method for preparing compounds of formula D is provided in Scheme 2. Boc-protected piperazine is coupled with hexafluoropropan-2-yl chloroformate to provide carbamate intermediate E. Removal of the Boc group with an acid, such as trifluoroacetic acid or HCl, affords intermediate F, which subsequently undergoes a reductive amination, using an agent such as sodium triacetoxyborohydride, with an aldehyde of formula A to lead to a piperazine carbamate of formula D.




Another method of preparing compounds of formula D is provided in Scheme 3. A compound of formula G, where X is a halogen, is coupled with compound H under palladium-mediated conditions to provide a piperazine carbamate of formula D.
Further Forms of Piperazine Carbamates Disclosed Herein
Isomers
Furthermore, in some embodiments, the compounds described herein exist as geometric isomers. In some embodiments, the compounds described herein possess one or more double bonds. The compounds presented herein include all cis, trans, syn, anti, entgegen (E), and zusammen (Z) isomers as well as the corresponding mixtures thereof. In some situations, compounds exist as tautomers. The compounds described herein include all possible tautomers within the formulas described herein. In some situations, the compounds described herein possess one or more chiral centers and each center exists in the R configuration, or S configuration. The compounds described herein include all diastereomeric, enantiomeric, and epimeric forms as well as the corresponding mixtures thereof. In additional embodiments of the compounds and methods provided herein, mixtures of enantiomers and/or diastereoisomers, resulting from a single preparative step, combination, or interconversion are useful for the applications described herein. In some embodiments, the compounds described herein are prepared as their individual stereoisomers by reacting a racemic mixture of the compound with an optically active resolving agent to form a pair of diastereoisomeric compounds, separating the diastereomers and recovering the optically pure enantiomers. In some embodiments, dissociable complexes are preferred (e.g., crystalline diastereomeric salts). In some embodiments, the diastereomers have distinct physical properties (e.g., melting points, boiling points, solubilities, reactivity, etc.) and are separated by taking advantage of these dissimilarities. In some embodiments, the diastereomers are separated by chiral chromatography, or preferably, by separation/resolution techniques based upon differences in solubility. In some embodiments, the optically pure enantiomer is then recovered, along with the resolving agent, by any practical means that would not result in racemization.
Labeled Compounds
In some embodiments, the compounds described herein exist in their isotopically-labeled forms. In some embodiments, the methods disclosed herein include methods of treating diseases by administering such isotopically-labeled compounds. In some embodiments, the methods disclosed herein include methods of treating diseases by administering such isotopically-labeled compounds as pharmaceutical compositions. Thus, in some embodiments, the compounds disclosed herein include isotopically-labeled compounds, which are identical to those recited herein, but for the fact that one or more atoms are replaced by an atom having an atomic mass or mass number different from the atomic mass or mass number usually found in nature. Examples of isotopes that are incorporated into compounds of the invention include isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorous, sulfur, fluorine and chloride, such as 2H, 3H, 13C, 14C, 15N, 18O, 17O, 31P, 32P, 35S, 18F, and 36Cl, respectively. Compounds described herein, and the pharmaceutically acceptable salts, esters, solvate, hydrates or derivatives thereof which contain the aforementioned isotopes and/or other isotopes of other atoms are within the scope of this invention. Certain isotopically-labeled compounds, for example those into which radioactive isotopes such as 3H and 14C are incorporated, are useful in drug and/or substrate tissue distribution assays. Tritiated, i. e., 3H and carbon-14, i. e., 14C, isotopes are particularly preferred for their ease of preparation and detectability. Further, substitution with heavy isotopes such as deuterium, i.e., 2H, produces certain therapeutic advantages resulting from greater metabolic stability, for example increased in vivo half-life or reduced dosage requirements. In some embodiments, the isotopically labeled compounds, pharmaceutically acceptable salt, ester, solvate, hydrate or derivative thereof is prepared by any suitable method.
In some embodiments, the compounds described herein are labeled by other means, including, but not limited to, the use of chromophores or fluorescent moieties, bioluminescent labels, or chemiluminescent labels.
Pharmaceutically Acceptable Salts
In some embodiments, the compounds described herein exist as their pharmaceutically acceptable salts. In some embodiments, the methods disclosed herein include methods of treating diseases by administering such pharmaceutically acceptable salts. In some embodiments, the methods disclosed herein include methods of treating diseases by administering such pharmaceutically acceptable salts as pharmaceutical compositions.
In some embodiments, the compounds described herein possess acidic or basic groups and therefore react with any of a number of inorganic or organic bases, and inorganic and organic acids, to form a pharmaceutically acceptable salt. In some embodiments, these salts are prepared in situ during the final isolation and purification of the compounds of the invention, or by separately reacting a purified compound in its free form with a suitable acid or base, and isolating the salt thus formed.
Solvates
In some embodiments, the compounds described herein exist as solvates. The invention provides for methods of treating diseases by administering such solvates. The invention further provides for methods of treating diseases by administering such solvates as pharmaceutical compositions.
Solvates contain either stoichiometric or non-stoichiometric amounts of a solvent, and, in some embodiments, are formed during the process of crystallization with pharmaceutically acceptable solvents such as water, ethanol, and the like. Hydrates are formed when the solvent is water, or alcoholates are formed when the solvent is alcohol. Solvates of the compounds described herein are conveniently prepared or formed during the processes described herein. By way of example only, hydrates of the compounds described herein are conveniently prepared by recrystallization from an aqueous/organic solvent mixture, using organic solvents including, but not limited to, dioxane, tetrahydrofuran or methanol. In addition, the compounds provided herein exist in unsolvated as well as solvated forms. In general, the solvated forms are considered equivalent to the unsolvated forms for the purposes of the compounds and methods provided herein.
Pharmaceutical Compositions
In certain embodiments, the piperazine carbamate as described herein is administered as a pure chemical. In other embodiments, the piperazine carbamate described herein is combined with a pharmaceutically suitable or acceptable carrier (also referred to herein as a pharmaceutically suitable (or acceptable) excipient, physiologically suitable (or acceptable) excipient, or physiologically suitable (or acceptable) carrier) selected on the basis of a chosen route of administration and standard pharmaceutical practice as described, for example, in Remington: The Science and Practice of Pharmacy (Gennaro, 21st Ed. Mack Pub. Co., Easton, Pa. (2005)).
Accordingly, provided herein is a pharmaceutical composition comprising at least one piperazine carbamate described herein, or a stereoisomer, pharmaceutically acceptable salt, hydrate, solvate, or N-oxide thereof, together with one or more pharmaceutically acceptable carriers. The carrier(s) (or excipient(s)) is acceptable or suitable if the carrier is compatible with the other ingredients of the composition and not deleterious to the recipient (i.e., the subject) of the composition.
One embodiment provides a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula (I), or a pharmaceutically acceptable salt thereof.
One embodiment provides a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula (Ia), or a pharmaceutically acceptable salt thereof.
One embodiment provides a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula (II), or a pharmaceutically acceptable salt thereof.
One embodiment provides a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula (IIa), or a pharmaceutically acceptable salt thereof.
One embodiment provides a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula (III), or a pharmaceutically acceptable salt thereof.
One embodiment provides a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula (IV), or a pharmaceutically acceptable salt thereof.
Another embodiment provides a pharmaceutical composition consisting essentially of a pharmaceutically acceptable carrier and a compound of Formula (I), or a pharmaceutically acceptable salt thereof. Another embodiment provides a pharmaceutical composition consisting essentially of a pharmaceutically acceptable carrier and a compound of Formula (Ia), or a pharmaceutically acceptable salt thereof. Another embodiment provides a pharmaceutical composition consisting essentially of a pharmaceutically acceptable carrier and a compound of Formula (II), or a pharmaceutically acceptable salt thereof. Another embodiment provides a pharmaceutical composition consisting essentially of a pharmaceutically acceptable carrier and a compound of Formula (IIa), or a pharmaceutically acceptable salt thereof. Another embodiment provides a pharmaceutical composition consisting essentially of a pharmaceutically acceptable carrier and a compound of Formula (III), or a pharmaceutically acceptable salt thereof. Another embodiment provides a pharmaceutical composition consisting essentially of a pharmaceutically acceptable carrier and a compound of Formula (IV), or a pharmaceutically acceptable salt thereof.
In certain embodiments, the piperazine carbamate as described herein is substantially pure, in that it contains less than about 5%, or less than about 1%, or less than about 0.1%, of other organic small molecules, such as contaminating intermediates or by-products that are created, for example, in one or more of the steps of a synthesis method.
These formulations include those suitable for oral, rectal, topical, buccal, parenteral (e.g., subcutaneous, intramuscular, intradermal, or intravenous) rectal, vaginal, or aerosol administration.
Methods
Disclosed herein are methods of modulating the activity of MAGL and/or ABHD6. Contemplated methods, for example, comprise exposing said enzyme to a compound described herein. In some embodiments, the compound utilized by one or more of the foregoing methods is one of the generic, subgeneric, or specific compounds described herein, such as a compound of Formula (I), (II), (III), or (IV). In some embodiments, the compound utilized by one or more of the foregoing methods is one of the generic, subgeneric, or specific compounds described herein, such as a compound of Formula (I), (Ia), (II), (IIa), (III), or (IV). The ability of compounds described herein to modulate or inhibit MAGL and/or ABHD6 is evaluated by procedures known in the art and/or described herein. Another aspect of this disclosure provides methods of treating a disease associated with expression or activity of MAGL and/or ABHD6 in a patient. For example, provided herein are compounds that are selective in inhibiting MAGL or ABHD6, or both, as compared to inhibition of other serine hydrolases e.g., FAAH, e.g., 10, 100, 1000 or more fold inhibition of MAGL over FAAH. In other embodiments, disclosed compounds are more selective in inhibition of MAGL as compared to ABHD6.
Also disclosed herein are methods of treating and/or preventing in a patient in need thereof a disorder such as one or more of acute or chronic pain and neuropathy. Disclosed methods include administering a pharmaceutically effective amount of a compound described herein.
In certain embodiments, a disclosed compound utilized by one or more of the foregoing methods is one of the generic, subgeneric, or specific compounds described herein, such as a compound of Formula (I), (II), (III), or (IV). In certain embodiments, a disclosed compound utilized by one or more of the foregoing methods is one of the generic, subgeneric, or specific compounds described herein, such as a compound of Formula (I), (Ia), (II), (IIa), (III), or (IV).
Disclosed compounds are administered to patients (animals and humans) in need of such treatment in dosages that will provide optimal pharmaceutical efficacy. It will be appreciated that the dose required for use in any particular application will vary from patient to patient, not only with the particular compound or composition selected, but also with the route of administration, the nature of the condition being treated, the age and condition of the patient, concurrent medication or special diets then being followed by the patient, and other factors, with the appropriate dosage ultimately being at the discretion of the attendant physician. For treating clinical conditions and diseases noted above, a contemplated compound disclosed herein is administered orally, subcutaneously, topically, parenterally, by inhalation spray or rectally in dosage unit formulations containing conventional non-toxic pharmaceutically acceptable carriers, adjuvants and vehicles. Parenteral administration include subcutaneous injections, intravenous or intramuscular injections or infusion techniques.
Also contemplated herein are combination therapies, for example, co-administering a disclosed compound and an additional active agent, as part of a specific treatment regimen intended to provide the beneficial effect from the co-action of these therapeutic agents. The beneficial effect of the combination includes, but is not limited to, pharmacokinetic or pharmacodynamic co-action resulting from the combination of therapeutic agents. Administration of these therapeutic agents in combination typically is carried out over a defined time period (usually weeks, months or years depending upon the combination selected). Combination therapy is intended to embrace administration of multiple therapeutic agents in a sequential manner, that is, wherein each therapeutic agent is administered at a different time, as well as administration of these therapeutic agents, or at least two of the therapeutic agents, in a substantially simultaneous manner.
Substantially simultaneous administration is accomplished, for example, by administering to the subject a single formulation or composition, (e.g., a tablet or capsule having a fixed ratio of each therapeutic agent or in multiple, single formulations (e.g., capsules) for each of the therapeutic agents. Sequential or substantially simultaneous administration of each therapeutic agent is effected by any appropriate route including, but not limited to, oral routes, intravenous routes, intramuscular routes, and direct absorption through mucous membrane tissues. The therapeutic agents are administered by the same route or by different routes. For example, a first therapeutic agent of the combination selected is administered by intravenous injection while the other therapeutic agents of the combination are administered orally. Alternatively, for example, all therapeutic agents are administered orally or all therapeutic agents are administered by intravenous injection.
Combination therapy also embraces the administration of the therapeutic agents as described above in further combination with other biologically active ingredients and non-drug therapies. Where the combination therapy further comprises a non-drug treatment, the non-drug treatment is conducted at any suitable time so long as a beneficial effect from the co-action of the combination of the therapeutic agents and non-drug treatment is achieved. For example, in appropriate cases, the beneficial effect is still achieved when the non-drug treatment is temporally removed from the administration of the therapeutic agents, perhaps by days or even weeks.
The components of the combination are administered to a patient simultaneously or sequentially. It will be appreciated that the components are present in the same pharmaceutically acceptable carrier and, therefore, are administered simultaneously. Alternatively, the active ingredients are present in separate pharmaceutical carriers, such as, conventional oral dosage forms, that are administered either simultaneously or sequentially.
For example, e.g., for contemplated treatment of pain, a disclosed compound is co-administered with another therapeutic for pain such as an opioid, a cannabinoid receptor (CB-1 or CB-2) modulator, a COX-2 inhibitor, acetaminophen, and/or a non-steroidal anti-inflammatory agent. Additional therapeutics e.g., for the treatment of pain that are co-administered, include morphine, codeine, hydromorphone, hydrocodone, oxymorphone, fentanyl, tramadol, and levorphanol.
Other contemplated therapeutics for co-administration include aspirin, naproxen, ibuprofen, salsalate, diflunisal, dexibuprofen, fenoprofen, ketoprofen, oxaprozin, loxoprofen, indomethacin, tolmetin, sulindac, etodolac, ketorolac, piroxicam, meloxicam, tenoxicam, droxicam, lornoxicam, celecoxib, parecoxib, rimonabant, and/or etoricoxic.
The following examples are provided merely as illustrative of various embodiments and shall not be construed to limit the invention in any way.
EXAMPLES
List of Abbreviations
As used above, and throughout the description of the invention, the following abbreviations, unless otherwise indicated, shall be understood to have the following meanings:

    
    
        ACN or MeCN acetonitrile
        Bn benzyl
        BOC or Boc tert-butyl carbamate
        t-Bu tert-butyl
        Cy cyclohexyl
        DCE dichloroethane (ClCH2CH2Cl)
        DCM dichloromethane (CH2Cl2)
        DIPEA or DIEA diisopropylethylamine
        DMAP 4-(N, N-dimethylamino)pyridine
        DMF dimethylformamide
        DMA N,N-dimethylacetamide
        DMSO dimethylsulfoxide
        equiv equivalent(s)
        Et ethyl
        Et2O diethyl ether
        EtOH ethanol
        EtOAc ethyl acetate
        HPLC high performance liquid chromatography
        Me methyl
        MeOH methanol
        MS mass spectroscopy
        NMR nuclear magnetic resonance
        RP-HPLC reverse phase-high pressure liquid chromatography
        TFA trifluoroacetic acid
        THF tetrahydrofuran
        TLC thin layer chromatography

I. Chemical Synthesis

    
    


Unless otherwise noted, reagents and solvents were used as received from commercial suppliers. Anhydrous solvents and oven-dried glassware were used for synthetic transformations sensitive to moisture and/or oxygen. Yields were not optimized. Reaction times are approximate and were not optimized. Column chromatography and thin layer chromatography (TLC) were performed on silica gel unless otherwise noted. Spectra are given in ppm (δ) and coupling constants (J) are reported in Hertz. For proton spectra the solvent peak was used as the reference peak.
Example 1
1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(2-(3-(prop-2-yn-1-yloxy)pyrrolidin-1-yl)-6-(trifluoromethyl)benzyl)piperazine-1-carboxylate




Step 1: Preparation of (S)-2-(3-hydroxypyrrolidin-1-yl)-6-(trifluoromethyl)benzaldehyde




A thick walled flask was charged with (3S)-pyrrolidin-3-ol hydrochloride (900 mg, 7.28 mmol) and K2CO3 (1839 mg, 13.33 mmol) and DMSO (10 mL). The contents were stirred for 5 min. 2-Fluoro-6-(trifluoromethyl)benzaldehyde (1280 mg, 6.66 mmol) was added and the reaction flask was sealed and heated to 125° C. for 30 min. The reaction was diluted in EtOAc (200 mL) and washed with 1N NaOH (2×100 mL) and brine (1×100 mL). The organics were dried over anhydrous Na2SO4 and concentrated. The resulting oil was chromatographed on a silica column (30% EtOAc in hexanes) yielding 2-[(3 S)-3-hydroxypyrrolidin-1-yl]-6-(trifluoromethyl)benzaldehyde (660 mg, 2.54 mmol, 38% yield) as an orange oil. 1H NMR (400 MHz, Chloroform-d) δ 10.37-10.20 (m, 1H), 7.42-7.28 (m, 1H), 7.05 (dd, J=25.7, 8.1 Hz, 2H), 4.59-4.40 (m, 1H), 3.67-3.28 (m, 3H), 3.17-2.98 (m, 1H), 2.69 (d, J=11.7 Hz, 1H), 2.14-1.92 (m, 2H). LCMS 260.0 [M+H]+.
Step 2: Preparation of tert-butyl (S)-4-(2-(3-hydroxypyrrolidin-1-yl)-6-(trifluoromethyl)benzyl)piperazine-1-carboxylate




A flask was charged with (S)-2-(3-hydroxypyrrolidin-1-yl)-6-(trifluoromethyl)benzaldehyde (630 mg, 2.43 mmol) and dissolved in DCM (50 mL). 4 Å Molecular sieves (630 mg) and tert-butyl piperazine-1-carboxylate (498 mg, 2.67 mmol) were added. After stirring 30 min at rt, NaBH(OAc)3 (669 mg, 3.16 mmol) was added and the reaction was stirred at rt for 18 h. The reaction was poured into DCM (150 mL) and washed with sat Na2CO3 (2×100 mL). The organics were dried over anhydrous Na2SO4, filtered and concentrated. The resulting oil was chromatographed on a silica column (0 to 50% EtOAc in hexane) and yielded tert-butyl (S)-4-(2-(3-hydroxypyrrolidin-1-yl)-6-(trifluoromethyl)benzyl)piperazine-1-carboxylate (724 mg, 69%) as a white solid. 1H NMR (400 MHz, Chloroform-d) δ 7.32-7.15 (m, 3H), 4.42-4.37 (m, 1H), 3.91 (br s, 1H), 3.72 (s, 2H), 3.43-3.29 (m, 5H), 3.26-3.11 (m, 2H), 3.01 (td, J=8.9, 4.6 Hz, 1H), 2.32-2.20 (m, 4H), 2.21-2.05 (m, 1H), 1.99-1.89 (m, 1H), 1.40 (s, 9H). LCMS (ESI, m/z): 430.2 [M+H]+.
Step 3: Preparation of tert-butyl (S)-4-(2-(3-(prop-2-yn-1-yloxy)pyrrolidin-1-yl)-6-(trifluoromethyl)benzyl)piperazine-1-carboxylate




A flask was charged with tert-butyl 4-[[2-[(3S)-3-hydroxypyrrolidin-1-yl]-6-(trifluoromethyl)phenyl]methyl]piperazine-1-carboxylate (724 mg, 1.69 mmol, azeotroped from toluene) and DMF (15 mL) and cooled to 0° C. Sodium hydride was added and the mixture was stirred for 30 min. Propargyl bromide (80% weight solution in toluene, 501 mg, 3.37 mmol) was added dropwise and the reaction was stirred at 0° C. for 1 h and rt for 2 h. The reaction was quenched with brine (50 mL) and diluted in EtOAc (150 mL). The organic phase was washed with brine (2×100 mL). The organics were dried over anhydrous Na2SO4, filtered, and concentrated. The resulting oil was chromatographed on a silica column with a gradient (0 to 25% EtOAc in hexane) and yielded tert-butyl 4-[[2-[(3S)-3-prop-2-ynoxypyrrolidin-1-yl]-6-(trifluoromethyl)phenyl]methyl]piperazine-1-carboxylate (220 mg, 0.470 mmol, 28% yield) as an orange oil. 1H NMR (400 MHz, Chloroform-d) δ 7.35-7.21 (m, 3H), 4.38 (tt, J=5.9, 3.3 Hz, 1H), 4.29-4.12 (m, 2H), 3.70 (s, 2H), 3.44 (dd, J=10.1, 5.7 Hz, 1H), 3.33 (s, 4H), 3.30-3.23 (m, 1H), 3.19-3.06 (m, 2H), 2.45 (td, J=2.4, 1.0 Hz, 1H), 2.30 (s, 4H), 2.17 (dq, J=14.3, 7.2 Hz, 1H), 2.09-1.97 (m, 1H), 1.44 (s, 9H). LCMS (ESI, m/z): 468.2 [M+H]+.
Step 4: Preparation of 1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(2-(3-(prop-2-yn-1-yloxy)pyrrolidin-1-yl)-6-(trifluoromethyl)benzyl)piperazine-1-carboxylate




A flask was charged with tert-butyl 4-[[2-[(3S)-3-prop-2-ynoxypyrrolidin-1-yl]-6-(trifluoromethyl)phenyl]methyl]piperazine-1-carboxylate (220 mg, 0.470 mmol) and dissolved in DCM (4 mL). TFA (1 mL) was added and the reaction was stirred at rt for 3 h. The reaction was concentrated and dissolved in DCM (75 mL) and washed with 1N NaOH (50 mL). The aqueous was extracted with DCM (2×50 mL). The organics were dried over anhydrous Na2SO4, filtered, and concentrated and yielded 1-[[2-[(3 S)-3-prop-2-ynoxypyrrolidin-1-yl]-6-(trifluoromethyl)phenyl]methyl]piperazine (170 mg, 0.463 mmol, 98% yield) which was carried on without further purification. A hexafluoropropan-2-yl chloroformate solution was prepared in situ by treating triphosgene (20 mg, 0.068 mmol) in DCM (3 mL) with hexafluoroisopropanol (21 μL, 0.204 mmol) and DIEA (95 μL, 0.544 mmol) for 2 h at rt. The chloroformate solution was added dropwise to the crude (S)-1-(2-(3-(prop-2-yn-1-yloxy)pyrrolidin-1-yl)-6-(trifluoromethyl)benzyl)piperazine (50 mg, 0.136 mmol) in DCM (3 mL). The reaction was stirred at rt for 18 h. The reaction was diluted in DCM (150 mL) and washed with sat Na2CO3 (2×50 mL). The organics were dried over anhydrous Na2SO4, filtered, and concentrated. The resulting oil was chromatographed on a silica column with a gradient (0 to 20% EtOAc in hexane) and yielded 1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(2-(3-(prop-2-yn-1-yloxy)pyrrolidin-1-yl)-6-(trifluoromethyl)benzyl)piperazine-1-carboxylate (63 mg, 82%) as an orange oil. 1H NMR (400 MHz, Chloroform-d) δ 7.40-7.26 (m, 3H), 5.76 (hept, J=6.1 Hz, 1H), 4.40 (tt, J=5.9, 3.1 Hz, 1H), 4.23-4.17 (m, 2H), 3.76 (s, 2H), 3.44 (dd, J=30.4, 5.1 Hz, 5H), 3.29 (q, J=7.6 Hz, 1H), 3.21-3.04 (m, 2H), 2.45 (dt, J=2.3, 1.2 Hz, 1H), 2.40 (dt, J=13.0, 4.6 Hz, 4H), 2.20 (dq, J=14.2, 7.2 Hz, 1H), 2.06 (ddt, J=12.6, 7.4, 4.2 Hz, 1H). LCMS (ESI, m/z): 562.2 [M+H]+.
Example 2
1,1,1,3,3,3-hexafluoropropan-2-yl (R)-4-(2-(3-(prop-2-yn-1-yloxy)pyrrolidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate




The title compound was prepared from (R)-pyrrolidin-3-ol and 2-fluoro-4-(trifluoromethyl)benzaldehyde according to the representative procedure of Example 1 Steps 1-4 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl (R)-4-(2-(3-(prop-2-yn-1-yloxy)pyrrolidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate as an orange oil. 1H NMR (400 MHz, Chloroform-d) δ 7.50 (d, J=7.8 Hz, 1H), 7.17-7.07 (m, 2H), 5.74 (hept, 1H), 4.39 (d, J=2.3 Hz, 1H), 4.19 (t, J=2.1 Hz, 2H), 3.64-3.52 (m, 5H), 3.51-3.45 (m, 2H), 3.41 (q, J=8.1, 7.6 Hz, 1H), 3.34 (d, J=10.3 Hz, 1H), 3.27-3.16 (m, 1H), 2.50-2.41 (m, 5H), 2.24-2.06 (m, 2H). LCMS (ESI, m/z): 562.2 [M+H]+.
Example 3
1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(4-chloro-2-(3-(prop-2-yn-1-yloxy)pyrrolidin-1-yl)benzyl)piperazine-1-carboxylate




The title compound was prepared from (S)-pyrrolidin-3-ol and 2-fluoro-4-chlorobenzaldehyde according to the representative procedure of Example 1 Steps 1-4 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(4-chloro-2-(3-(prop-2-yn-1-yloxy)pyrrolidin-1-yl)benzyl)piperazine-1-carboxylate as an orange oil. 1H NMR (400 MHz, Chloroform-d) δ 7.20 (d, J=8.7 Hz, 1H), 6.83-6.71 (m, 2H), 5.68 (hept, J=6.2 Hz, 1H), 4.30 (tt, J=5.9, 3.2 Hz, 1H), 4.11 (d, J=2.4 Hz, 2H), 3.54-3.35 (m, 7H), 3.34-3.22 (m, 2H), 3.11 (ddd, J=8.9, 7.7, 4.9 Hz, 1H), 2.44-2.29 (m, 5H), 2.07 (dt, J=13.6, 6.7 Hz, 1H), 2.02-1.89 (m, 1H). LCMS (ESI, m/z): 528.1 [M+H]+.
Example 4
1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(4-chloro-2-(3-(fluoromethyl)pyrrolidin-1-yl)benzyl)piperazine-1-carboxylate




Step 1: Preparation of tert-butyl (S)-4-(4-chloro-2-(3-((tosyloxy)methyl)pyrrolidin-1-yl)benzyl)piperazine-1-carboxylate




A flask was charged with tert-butyl 4-[[4-chloro-2-[(3S)-3-(hydroxymethyl)pyrrolidin-1-yl]phenyl]methyl]piperazine-1-carboxylate (2.8 g, 6.83 mmol) [prepared from (S)-pyrrolidin-3-ylmethanol and tert-butyl piperazine-1-carboxylate according to the representative procedure of Example 1 Steps 1-2], DCM (100 mL), and pyridine (1.7 mL, 20.5 mmol). DMAP (83 mg, 0.680 mmol) was added and the mixture was cooled to 0° C. 4-methylbenzenesulfonyl chloride (1.56 g, 8.2 mmol) was added and the reaction was refluxed for 18 h. The reaction was diluted in DCM (150 mL) and washed with brine (3×100 mL). The organics were dried over anhydrous Na2SO4 and concentrated. The resulting oil was chromatographed on a silica column with a gradient (0 to 20% EtOAc in hexane) and yielded tert-butyl (S)-4-(4-chloro-2-(3-((tosyloxy)methyl)pyrrolidin-1-yl)benzyl)piperazine-1-carboxylate (1.89 g, 3.35 mmol, 49% yield) as an orange oil. 1H NMR (400 MHz, Chloroform-d) δ 7.81 (d, J=8.3 Hz, 2H), 7.36 (d, J=8.0 Hz, 2H), 7.31 (s, 1H), 6.88 (dd, J=8.2, 2.0 Hz, 1H), 6.79 (d, J=2.0 Hz, 1H), 4.11-3.98 (m, 2H), 3.41 (q, J=13.6 Hz, 6H), 3.27-3.08 (m, 3H), 3.07-2.93 (m, 1H), 2.63 (hept, J=7.0 Hz, 1H), 2.46 (s, 3H), 2.35 (t, J=4.5 Hz, 4H), 2.06 (dtd, J=12.8, 7.9, 5.0 Hz, 1H), 1.73 (s, OH), 1.62 (dq, J=14.1, 7.3 Hz, 1H), 1.48 (s, 9H). LCMS (ESI, m/z): 564.3 [M+H]+.
Step 2: Preparation of 1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(4-chloro-2-(3-((tosyloxy)methyl)pyrrolidin-1-yl)benzyl)piperazine-1-carboxylate




A flask was charged with tert-butyl 4-[[4-chloro-2-[(3S)-3-(p-tolylsulfonyloxymethyl)pyrrolidin-1-yl]phenyl]methyl]piperazine-1-carboxylate (945 mg, 1.68 mmol), DCM (50 mL), and 4-methylmorpholine (0.64 mL, 5.03 mmol). The reaction was cooled to 0° C. and iodo(trimethyl)silane (0.29 mL, 2.01 mmol) was added dropwise. After stirring 30 min at 0° C., the reaction was diluted in DCM and washed with brine (3×). The organics were dried over anhydrous Na2SO4 and concentrated. Crude [(3S)-1-[5-chloro-2-(piperazin-1-ylmethyl)phenyl]pyrrolidin-3-yl]methyl 4-methylbenzenesulfonate (774 mg, 1.67 mmol, 99% yield) was carried on without further purification. A hexafluoropropan-2-yl chloroformate solution was formed in situ by treating triphosgene (165 mg, 0.560 mmol) in DCM (50 mL) with hexafluoroisopropanol (210 μL, 2.04 mmol) and DIEA (870 μL, 5.01 mmol) for 2 h at rt and added dropwise to the crude tosylate solution in DCM (50 mL). The reaction was stirred at rt for 18 h. The reaction was diluted in DCM (150 mL) and washed with sat Na2CO3 (2×100 mL). The organics were dried over anhydrous Na2SO4, filtered, and concentrated. The resulting oil was chromatographed on a silica column with a gradient (0 to 20% EtOAc in hexanes) and yielded [2,2,2-trifluoro-1-(trifluoromethyl)ethyl]4-[[4-chloro-2-[(3S)-3-(p-tolylsulfonyloxymethyl)pyrrolidin-1-yl]phenyl]methyl]piperazine-1-carboxylate (580 mg, 0.881 mmol, 52% yield) as a yellow oil. 1H NMR (400 MHz, Chloroform-d) δ 7.82 (d, J=8.3 Hz, 2H), 7.37 (d, J=8.0 Hz, 2H), 7.30 (d, J=8.1 Hz, 1H), 6.90 (dd, J=8.2, 2.0 Hz, 1H), 6.82 (d, J=2.1 Hz, 1H), 5.77 (hept, J=6.3 Hz, 1H), 4.10-4.00 (m, 2H), 3.59-3.39 (m, 6H), 3.27-3.04 (m, 4H), 2.64 (dq, J=13.6, 6.3 Hz, 1H), 2.50-2.40 (m, 7H), 2.15-2.02 (m, 1H), 1.64 (dd, J=12.8, 6.6 Hz, 1H). LCMS (ESI, m/z): 658.1 [M+H]+.
Step 3: Preparation of 1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(4-chloro-2-(3-(fluoromethyl)pyrrolidin-1-yl)benzyl)piperazine-1-carboxylate




A vial was charged with 1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(4-chloro-2-(3-((tosyloxy)methyl)pyrrolidin-1-yl)benzyl)piperazine-1-carboxylate (52 mg, 0.079 mmol) and dissolved in THF (1 mL). A TBAF solution (1M in THF, 158 μL, 0.158 mmol) was added and the reaction was stirred at rt for 18 h. Additional TBAF (200 μL, 0.200 mmol) was added and the reaction was stirred at 60° C. for 2 h. The reaction was concentrated and purified by silica chromatography (0 to 30% EtOAc in hexane) yielding [2,2,2-trifluoro-1-(trifluoromethyl)ethyl]4-[[4-chloro-2-[(3S)-3-(fluoromethyl)pyrrolidin-1-yl]phenyl]methyl]piperazine-1-carboxylate (12 mg, 30%) as a clear oil. 1H NMR (400 MHz, Chloroform-d) δ 7.37-7.28 (m, 1H), 6.96-6.88 (m, 2H), 5.85-5.72 (m, 1H), 4.59-4.47 (m, 1H), 4.47-4.34 (m, 1H), 3.61-3.53 (m, 5H), 3.53-3.44 (m, 1H), 3.33-3.16 (m, 4H), 2.71 (tt, J=13.9, 6.7 Hz, 1H), 2.51-2.45 (m, 4H), 2.17-2.07 (m, 1H), 1.78-1.68 (m, 1H). LCMS (ESI, m/z): 506.1 [M+H]+.
Example 5
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-(methoxycarbonyl)-4-morpholinobenzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available methyl 5-formyl-2-morpholinobenzoate and tert-butyl piperazine-1-carboxylate according to the representative procedure of Example 1, Steps 2 and 4 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-(methoxycarbonyl)-4-morpholinobenzyl)piperazine-1-carboxylate as a clear oil: 1H NMR (400 MHz, Chloroform-d) δ 7.56 (s, 1H), 7.26 (dd, J=8.3, 2.2 Hz, 1H), 6.83 (d, J=7.7 Hz, 1H), 5.62 (p, J=6.2 Hz, 1H), 3.77 (s, 3H), 3.76-3.70 (m, 4H), 3.43 (dq, J=5.7, 3.4 Hz, 4H), 3.35 (s, 2H), 2.95-2.88 (m, 4H), 2.31 (dt, J=11.5, 5.0 Hz, 4H). LCMS (ESI, m/z): 514.0 [M+H]+.
Example 6
1,1,1,3,3,3-hexafluoropropan-2-yl 4-((3-(prop-2-yn-1-yloxy)-[1,1′-biphenyl]-4-yl)methyl)piperazine-1-carboxylate




A vial was charged with [2,2,2-trifluoro-1-(trifluoromethyl)ethyl]4-[(2-hydroxy-4-phenyl-phenyl)methyl]piperazine-1-carboxylate (23 mg, 0.050 mmol), cesium carbonate (32 mg, 0.100 mmol) and dissolved in DMSO (2 mL). The reaction was stirred at rt for 2 min and then a propargyl bromide solution (80% in toluene, 0.02 mL, 0.130 mmol) was added dropwise. After 10 min the reaction was diluted in EtOAc (100 mL) and washed with brine (2×50 mL). The organics were dried over anhydrous Na2SO4, filtered, and concentrated. The resulting oil was chromatographed on a silica column with a gradient (0 to 35% EtOAc in hexane) and yielded [2,2,2-trifluoro-1-(trifluoromethyl)ethyl]4-[(4-phenyl-2-prop-2-ynoxy-phenyl)methyl]piperazine-1-carboxylate (23 mg, 0.045 mmol, 90% yield) as a clear oil. 1H NMR (400 MHz, Chloroform-d) δ 7.62 (d, J=7.8 Hz, 2H), 7.52-7.35 (m, 4H), 7.27-7.22 (m, 2H), 5.78 (hept, J=6.2 Hz, 1H), 4.82 (d, J=2.3 Hz, 2H), 3.68 (s, 2H), 3.65-3.56 (m, 4H), 2.59-2.53 (m, 5H). LCMS (ESI, m/z): 501.1 [M+H]+.
Example 7
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-((4-acetylpiperazin-1-yl)methyl)-2-methoxybenzyl)piperazine-1-carboxylate




Step 1: Preparation of 1-(4-(3-chloro-2-methoxybenzyl)piperazin-1-yl)ethan-1-one




A vial was charged with 3-chloro-2-methoxybenzaldehyde (350 mg, 2.05 mmol), 1-(piperazin-1-yl)ethan-1-one (289 mg, 2.26 mmol), and DCM (3 mL). 4 Å molecular sieves (300 mg) were added and the vial was purged with N2 and stirred at rt for 2 h. At that point, NaBH(OAc)3 (506 mg, 2.39 mmol) was added. The reaction was allowed to stir at rt overnight. The reaction was filtered over Celite, rinsed with MeOH, concentrated and taken up in EtOAc. The organic layer was washed 3 times with saturated NaHCO3, dried over Na2SO4 and concentrated to yield an oil. The oil was purified on silica gel by flash column chromatography to afford 1-(4-(3-chloro-2-methoxybenzyl)piperazin-1-yl)ethan-1-one (419 mg, 72% yield). 1H NMR (400 MHz, Chloroform-d) δ 7.32-7.29 (m, 1H), 7.29-7.26 (m, 1H), 7.12-6.99 (m, 1H), 3.92-3.86 (m, 3H), 3.68-3.60 (m, 2H), 3.60-3.54 (m, 2H), 3.49-3.41 (m, 2H), 2.53-2.43 (m, 4H), 2.13-2.05 (m, 3H).
Step 2: Preparation of potassium trifluoro((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)borate




A flask was charged with THF (40 mL) and 1,1,1,3,3,3-hexafluoropropan-2-yl piperazine-1-carboxylate (11.5 g, 41.1 mmol) and potassium (bromomethyl)trifluoroborate (7.89 g, 39.6 mmol) was added. The solution was heated at 80° C. for 3 h and concentrated. The residue was dissolved in acetone (300 mL) and MeOH (500 mL). Potassium carbonate (5.67 g, 41.1 mmol) was added and the solution was stirred at rt for 30 min. The reaction was filtered and the filtrate was concentrated. The resulting residue was re-suspended in a mixture of MeOH (150 mL), hexane (20 mL), and EtOAc (30 mL) resulting in a cloudy solution that partitioned into 2 layers. The top clear yellow layer was decanted and discarded. The remaining solids were dried under reduced pressure yielding trifluoro((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)borate (4.5 g, 12.5 mmol, 32% yield) as a white amorphous solid. 1H NMR (400 MHz, DMSO-d6) δ 6.57 (hept, J=6.4 Hz, 1H), 3.40-3.43 (m, 4H), 2.33-2.37 (m, 4H), 1.32-1.36 (m, 2H).
Step 3: Preparation of 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-((4-acetylpiperazin-1-yl)methyl)-2-methoxybenzyl)piperazine-1-carboxylate




A vial was charged with 1-(4-(3-chloro-2-methoxybenzyl)piperazin-1-yl)ethan-1-one (53.9 mg, 0.191 mmol) and potassium trifluoro((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)borate (91 mg, 0.229 mmol). To this vial was added Pd(OAc)2 (1.7 mg, 0.04 mmol), XPhos (7.3 mg, 0.08 mmol), and Cs2CO3 (186 mg, 0.572 mmol). The vial was flushed with nitrogen and evacuated 3 times. A 10:1 mixture of THF:H2O (5 mL) was then added by syringe. The resulting mixture was heated at 83° C. for 20 h. The reaction was then cooled to rt whereupon 5 mL H2O was added. The reaction was extracted with EtOAc (3×10 mL) and the combined organic layers were dried over Na2SO4, filtered and concentrated to yield an oil. The oil was purified on silica gel by flash column chromatography to afford 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-((4-acetylpiperazin-1-yl)methyl)-2-methoxybenzyl)piperazine-1-carboxylate (35 mg, 34% yield) as an oil: 1H NMR (400 MHz, Chloroform-d) δ 7.38-7.31 (m, 2H), 7.16-7.07 (m, 1H), 5.85-5.68 (m, 1H), 3.83 (s, 3H), 3.73-3.51 (m, 9H), 3.53-3.41 (m, 2H), 2.61-2.41 (m, 8H), 2.17-2.03 (m, 3H), 1.60 (s, 2H). LCMS (ESI, m/z): 541.3 [M+H]+.
Example 8
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-methyl-3-((4-(methylsulfonyl)piperazin-1-yl)methyl)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 3-chloro-2-methylbenzaldehyde and 1-(methylsulfonyl)piperazine according to the representative procedure of Example 7, Steps 1-3 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-methyl-3-((4-(methylsulfonyl)piperazin-1-yl)methyl)benzyl)piperazine-1-carboxylate as a clear oil: 1H NMR (400 MHz, Chloroform-d) δ 7.20-7.13 (m, 2H), 7.13-7.03 (m, 1H), 5.81-5.66 (m, 1H), 3.55-3.46 (m, 7H), 3.27-3.15 (m, 4H), 2.76 (s, 3H), 2.62-2.50 (m, 5H), 2.50-2.40 (m, 3H), 2.39 (s, 1H), 2.33 (s, 3H). LCMS (ESI, m/z): 561.2 [M+H]+.
Example 9
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-((4-fluoropiperidin-1-yl)methyl)-2-methoxybenzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 3-chloro-2-methoxybenzaldehyde and 4-fluoropiperidine according to the representative procedure of Example 7, Steps 1-3 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-((4-fluoropiperidin-1-yl)methyl)-2-methoxybenzyl)piperazine-1-carboxylate as a clear oil: 1H NMR (400 MHz, Chloroform-d) δ 7.30-7.24 (m, 1H), 7.25-7.20 (m, 1H), 7.04-6.98 (m, 1H), 5.75-5.60 (m, 1H), 4.74-4.47 (m, 1H), 3.80-3.69 (m, 3H), 3.57-3.38 (m, 8H), 2.57 (s, 2H), 2.52-2.37 (m, 4H), 2.32 (s, 2H), 1.94-1.70 (m, 4H). LCMS (ESI, m/z): 516.2 [M+H]+.
Example 10
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-methyl-3-((4-(methylsulfonamido)piperidin-1-yl)methyl)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 3-chloro-2-methylbenzaldehyde and N-piperidin-yl-methansulfonamide according to the representative procedure of Example 7, Steps 1-3 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-methyl-3-((4-(methylsulfonamido)piperidin-1-yl)methyl)benzyl)piperazine-1-carboxylate as a clear oil: 1H NMR (400 MHz, Chloroform-d) δ 7.22-7.14 (m, 2H), 7.14-7.07 (m, 1H), 5.85-5.72 (m, 1H), 4.20-4.14 (m, 1H), 3.59-3.49 (m, 5H), 3.49-3.43 (m, 2H), 3.43-3.32 (m, 1H), 3.01 (s, 3H), 2.88-2.74 (m, 2H), 2.54-2.41 (m, 4H), 2.38-2.31 (m, 3H), 2.21-2.09 (m, 2H), 2.01-1.94 (m, 2H), 1.63-1.47 (m, 3H). LCMS (ESI, m/z): 575.2 [M+H]+.
Example 11
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-((4-acetamidopiperidin-1-yl)methyl)-2-methylbenzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 3-chloro-2-methylbenzaldehyde and 4-acetomidopiperidine according to the representative procedure of Example 7, Steps 1-3 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-((4-acetamidopiperidin-1-yl)methyl)-2-methylbenzyl)piperazine-1-carboxylate as a clear oil: 1H NMR (400 MHz, Chloroform-d) δ 7.24-7.20 (m, 1H), 7.16-6.98 (m, 2H), 5.75-5.63 (m, 1H), 5.40 (s, 1H), 3.79 (s, 1H), 3.69-3.26 (m, 8H), 2.87 (s, 2H), 2.49-2.33 (m, 4H), 2.33-2.07 (m, 5H), 1.95-1.79 (m, 5H), 1.52 (s, 2H). LCMS (ESI, m/z): 539.2 [M+H]+.
Example 12
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-((4,4-difluoropiperidin-1-yl)methyl)-2-methylbenzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 3-chloro-2-methylbenzaldehyde and 4,4-difluoropiperidine according to the representative procedure of Example 7, Steps 1-3 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-((4,4-difluoropiperidin-1-yl)methyl)-2-methylbenzyl)piperazine-1-carboxylate as a clear oil: 1H NMR (400 MHz, Chloroform-d) δ 7.20-7.13 (m, 2H), 7.11-7.06 (m, 1H), 5.80-5.64 (m, 1H), 3.55-3.49 (m, 6H), 3.49 (s, 2H), 2.57-2.50 (m, 4H), 2.49-2.40 (m, 4H), 2.33 (s, 3H), 2.03-1.87 (m, 4H). LCMS (ESI, m/z): 518.2 [M+H]+.
Example 13
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-methyl-3-(pyrrolidin-1-ylmethyl)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 3-chloro-2-methylbenzaldehyde and pyrrolidine according to the representative procedure of Example 7, Steps 1-3 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-methyl-3-(pyrrolidin-1-ylmethyl)benzyl)piperazine-1-carboxylate as a clear oil: 1H NMR (400 MHz, Chloroform-d) δ 7.24-7.20 (m, 1H), 7.15-7.05 (m, 2H), 5.82-5.66 (m, 1H), 3.63-3.57 (m, 2H), 3.55-3.49 (m, 4H), 3.49-3.44 (m, 2H), 2.57-2.48 (m, 4H), 2.48-2.38 (m, 4H), 2.34 (s, 3H), 1.81-1.72 (m, 4H). LCMS (ESI, m/z): 468.2 [M+H]+.
Example 14
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-((4-acetylpiperazin-1-yl)methyl)-2-methylbenzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 3-chloro-2-methylbenzaldehyde and 1-(piperazin-1-yl)ethan-1-one according to the representative procedure of Example 7, Steps 1-3 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-((4-acetylpiperazin-1-yl)methyl)-2-methylbenzyl)piperazine-1-carboxylate as a clear oil: 1H NMR (400 MHz, Chloroform-d) δ 7.13-6.93 (m, 3H), 5.69-5.54 (m, 1H), 3.55-3.23 (m, 11H), 2.38-2.28 (m, 7H), 2.24 (s, 3H), 1.96 (s, 3H). LCMS (ESI, m/z): 524.1 [M+H]+.
Example 15
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-((4-fluoropiperidin-1-yl)methyl)-2-methylbenzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 3-chloro-2-methylbenzaldehyde and 4-fluoropiperidine hydrochloride according to the representative procedure of Example 7, Steps 1-3 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-((4-fluoropiperidin-1-yl)methyl)-2-methylbenzyl)piperazine-1-carboxylate as a clear oil: 1H NMR (400 MHz, Chloroform-d) δ 7.21-7.16 (m, 1H), 7.15-7.11 (m, 1H), 7.10-7.05 (m, 1H), 5.81-5.67 (m, 1H), 4.80-4.54 (m, 1H), 3.58-3.46 (m, 6H), 3.45 (s, 2H), 2.66-2.51 (m, 2H), 2.51-2.39 (m, 4H), 2.39-2.26 (m, 5H), 1.95-1.74 (m, 4H). LCMS (ESI, m/z): 500.1 [M+H]+.
Example 16
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-((4,4-difluoropiperidin-1-yl)methyl)-2-methoxybenzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 3-chloro-2-methoxybenzaldehyde and 4,4-difluoropiperidine according to the representative procedure of Example 7, Steps 1-3 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-((4,4-difluoropiperidin-1-yl)methyl)-2-methoxybenzyl)piperazine-1-carboxylate as a clear oil: 1H NMR (400 MHz, Chloroform-d) δ 7.28-7.21 (m, 2H), 7.05-6.99 (m, 1H), 5.77-5.60 (m, 1H), 3.78-3.68 (m, 3H), 3.57-3.40 (m, 8H), 2.57-2.39 (m, 8H), 2.02-1.81 (m, 4H). LCMS (ESI, m/z): 534.2 [M+H]+.
Example 17
1,1,1,3,3,3-hexafluoropropan-2-yl (R)-4-(3-((3-acetamidopyrrolidin-1-yl)methyl)-2-methylbenzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 3-chloro-2-methylbenzaldehyde and (R)—N-(pyrrolidin-3-yl)acetamide according to the representative procedure of Example 7, Steps 1-3 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl (R)-4-(3-((3-acetamidopyrrolidin-1-yl)methyl)-2-methylbenzyl)piperazine-1-carboxylate as a clear oil: 1H NMR (400 MHz, Chloroform-d) δ 7.12-7.04 (m, 2H), 7.03-6.97 (m, 1H), 5.76-5.59 (m, 2H), 4.41-4.26 (m, 1H), 3.52 (s, 2H), 3.47-3.38 (m, 6H), 2.83-2.72 (m, 1H), 2.49 (s, 2H), 2.41-2.30 (m, 4H), 2.25 (s, 3H), 2.23-2.14 (m, 2H), 1.88-1.82 (m, 3H), 1.50 (s, 1H). LCMS (ESI, m/z): 524.2 [M+H]+.
Example 18
1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(3-((3-acetamidopyrrolidin-1-yl)methyl)-2-methylbenzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 3-chloro-2-methylbenzaldehyde and (S)—N-(pyrrolidin-3-yl)acetamide according to the representative procedure of Example 7, Steps 1-3 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(3-((3-acetamidopyrrolidin-1-yl)methyl)-2-methylbenzyl)piperazine-1-carboxylate as a clear oil: 1H NMR (400 MHz, Chloroform-d) δ 7.15-7.07 (m, 2H), 7.06-7.00 (m, 1H), 5.80-5.59 (m, 2H), 4.44-4.31 (m, 1H), 3.55 (s, 2H), 3.50-3.41 (m, 6H), 2.87-2.75 (m, 1H), 2.52 (s, 2H), 2.44-2.34 (m, 4H), 2.28 (s, 3H), 2.24 (d, J=7.4 Hz, 2H), 1.87 (s, 3H), 1.53 (s, 1H). LCMS (ESI, m/z): 524.2 [M+H]+.
Example 19
1,1,1,3,3,3-hexafluoropropan-2-yl (R)-4-(2-methyl-3-((2-methylpiperidin-1-yl)methyl)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 3-chloro-2-methylbenzaldehyde and (R)-2-methylpiperidine according to the representative procedure of Example 7, Steps 1-3 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl (R)-4-(2-methyl-3-((2-methylpiperidin-1-yl)methyl)benzyl)piperazine-1-carboxylate as a clear oil: 1H NMR (400 MHz, Chloroform-d) δ 7.38 (s, 1H), 7.23-7.08 (m, 2H), 5.84-5.71 (m, 1H), 4.15 (s, 1H), 3.63-3.46 (m, 6H), 3.22 (s, 1H), 2.77 (s, 1H), 2.51-2.43 (m, 4H), 2.40 (s, 3H), 2.05 (s, 1H), 1.79-1.64 (m, 2H), 1.63-1.47 (m, 3H), 1.43-1.12 (m, 5H). LCMS (ESI, m/z): 496.6 [M+H]+.
Example 20
1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(2-methyl-3-((2-methylpiperidin-1-yl)methyl)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 3-chloro-2-methylbenzaldehyde and (S)-2-methylpiperidine according to the representative procedure of Example 7, Steps 1-3 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(2-methyl-3-((2-methylpiperidin-1-yl)methyl)benzyl)piperazine-1-carboxylate as a clear oil: 1H NMR (400 MHz, Chloroform-d) δ 7.12-7.10 (m, 1H), 6.97-6.89 (m, 2H), 5.66-5.52 (m, 1H), 3.87 (d, J=13.4 Hz, 1H), 3.40-3.30 (m, 5H), 2.92-2.82 (m, 1H), 2.54-2.43 (m, 1H), 2.33-2.24 (m, 4H), 2.19 (s, 3H), 1.83-1.70 (m, 1H), 1.52-1.44 (m, 2H), 1.41 (s, 2H), 1.34-1.08 (m, 4H), 1.00 (d, J=6.2 Hz, 3H). LCMS (ESI, m/z): 496.6 [M+H]+.
Example 21
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-methyl-3-(morpholinomethyl)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 3-chloro-2-methylbenzaldehyde and morpholine according to the representative procedure of Example 7, Steps 1-3 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-methyl-3-(morpholinomethyl)benzyl)piperazine-1-carboxylate: 1H NMR (400 MHz, Chloroform-d) δ 7.26-7.16 (m, 2H), 7.15-7.06 (m, 1H), 5.79 (hept, J=6.3 Hz, 1H), 3.75-3.65 (m, 4H), 3.60-3.51 (m, 6H), 3.50 (s, 2H), 2.53-2.41 (m, 8H), 2.39 (s, 3H). LCMS (ESI, m/z): 484.2 [M+H]+.
Example 22
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-fluoro-3-(morpholinomethyl)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 3-chloro-2-fluorobenzaldehyde and morpholine according to the representative procedure of Example 7, Steps 1-3 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-fluoro-3-(morpholinomethyl)benzyl)piperazine-1-carboxylate: 1H NMR (400 MHz, Chloroform-d) δ 7.37-7.24 (m, 2H), 7.16-7.04 (m, 1H), 5.83-5.68 (m, 1H), 3.76-3.68 (m, 4H), 3.65-3.52 (m, 8H), 2.54-2.42 (m, 8H). LCMS (ESI, m/z): 488.2 [M+H]+.
Example 23
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(4-(morpholinomethyl)-2-(trifluoromethoxy)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 4-chloro-2-(trifluoromethoxy)benzaldehyde and morpholine according to the representative procedure of Example 7, Steps 1-3 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(4-(morpholinomethyl)-2-(trifluoromethoxy)benzyl)piperazine-1-carboxylate as an oil: 1H NMR (400 MHz, Chloroform-d) δ 7.46 (s, 1H), 7.32-7.21 (m, 2H), 5.83-5.68 (m, 1H), 3.79-3.67 (m, 4H), 3.62-3.55 (m, 6H), 3.52 (s, 2H), 2.56-2.40 (m, 8H). LCMS (ESI, m/z): 554.0 [M+H]+.
Example 24
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(4-(piperidin-1-ylmethyl)-2-(trifluoromethoxy)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 4-chloro-2-(trifluoromethoxy)benzaldehyde and piperidine according to the representative procedure of Example 7, Steps 1-3 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(4-(piperidin-1-ylmethyl)-2-(trifluoromethoxy)benzyl)piperazine-1-carboxylate as an oil: 1H NMR (400 MHz, Chloroform-d) δ 7.47-7.40 (m, 1H), 7.28-7.23 (m, 2H), 5.83-5.70 (m, 1H), 3.63-3.53 (m, 6H), 3.49 (s, 2H), 2.56-2.44 (m, 4H), 2.44-2.31 (m, 4H), 1.63-1.54 (m, 4H), 1.53-1.35 (m, 2H). LCMS (ESI, m/z): 552.2 [M+H]+.
Example 25
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(4-(pyrrolidin-1-ylmethyl)-2-(trifluoromethoxy)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 4-chloro-2-(trifluoromethoxy)benzaldehyde and pyrrolidine according to the representative procedure of Example 7, Steps 1-3 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(4-(pyrrolidin-1-ylmethyl)-2-(trifluoromethoxy)benzyl)piperazine-1-carboxylate as an oil: 1H NMR (400 MHz, Chloroform-d) δ 7.49-7.39 (m, 1H), 7.30-7.22 (m, 2H), 5.83-5.69 (m, 1H), 3.64 (s, 2H), 3.62-3.53 (m, 6H), 2.58-2.45 (m, 8H), 1.85-1.79 (m, 4H).
Example 26
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-(8-oxa-3-azabicyclo[3.2.1]octan-3-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate




Step 1: Preparation of tert-butyl 4-(3-bromo-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 3-bromo-4-(trifluoromethyl)benzaldehyde and tert-butyl piperazine-1-carboxylate according to the representative procedure of Example 1, Step 2 to provide tert-butyl 4-(3-bromo-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate as a pale oil: 1H NMR (400 MHz, Chloroform-d) δ 7.72 (s, 1H), 7.70-7.57 (m, 1H), 7.43-7.32 (m, 1H), 3.57-3.37 (m, 6H), 2.49-2.30 (m, 4H), 1.47 (s, 9H).
Step 2: Preparation of tert-butyl 4-(3-(8-oxa-3-azabicyclo[3.2.1]octan-3-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate




A vial was charged with tert-butyl 4-(3-bromo-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate (296 mg, 0.699 mmol). To this vial was added Pd(OAc)2 (9.4 mg, 0.042 mmol), RuPhos (78.4 mg, 0.168 mmol), and sodium tert-butoxide (201 mg, 2.098 mmol). The vial was flushed with nitrogen and evacuated 3 times. 8-Oxa-3-azabicyclo[3.2.1]octane (271 mg, 2.098 mmol) was added to the vial, followed by anhydrous THF (3 mL). The resulting stirred mixture was heated at 83° C. for 18 h. The reaction was cooled to rt whereupon 5 mL H2O was added. The reaction mixture was extracted with EtOAc (3×10 mL) and the combined organic layers were dried over Na2SO4, filtered and concentrated to yield an oil. The oil was purified on silica gel by flash column chromatography to afford tert-butyl 4-(3-(8-oxa-3-azabicyclo[3.2.1]octan-3-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate as a clear oil (140 mg, 43% yield). 1H NMR (400 MHz, Chloroform-d) δ 7.61-7.55 (m, 1H), 7.48-7.43 (m, 1H), 7.25-7.19 (m, 1H), 4.44-4.36 (m, 2H), 3.56-3.50 (m, 2H), 3.50-3.40 (m, 4H), 3.17-3.11 (m, 2H), 2.75-2.69 (m, 2H), 2.44-2.34 (m, 4H), 2.25-2.16 (m, 2H), 1.98-1.88 (m, 2H), 1.47 (s, 9H). LCMS (ESI, m/z): 456.2 [M+H]+.
Step 3: Preparation of 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-(8-oxa-3-azabicyclo[3.2.1]octan-3-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate




tert-Butyl 4-(3-(8-oxa-3-azabicyclo[3.2.1]octan-3-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate was added to a vial and stirred with 3 mL DCM. The vial was cooled to 0° C. by ice bath and 4 N HCl in dioxane (0.73 mL, 6.0 eq) was added dropwise. The mixture was stirred at rt overnight. Solvent was removed and the resulting solid was used without further purification according to the representative procedure of Example 1, Step 4 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-(8-oxa-3-azabicyclo[3.2.1]octan-3-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate as a clear oil (69 mg, 26% yield). 1H NMR (400 MHz, Chloroform-d) δ 7.64-7.58 (m, 1H), 7.46 (s, 1H), 7.22 (d, J=8.1 Hz, 1H), 5.77 (hept, J=6.2 Hz, 1H), 4.46-4.35 (m, 2H), 3.64-3.53 (m, 6H), 3.18-3.09 (m, 2H), 2.77-2.68 (m, 2H), 2.53-2.40 (m, 4H), 2.25-2.16 (m, 2H), 2.00-1.88 (m, 2H). LCMS (ESI, m/z): 550.2 [M+H]+.
Example 27
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(4-(8-oxa-3-azabicyclo[3.2.1]octan-3-yl)-2-fluorobenzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 4-chloro-2-fluorobenzaldehyde and 8-oxa-3-azabicylco[3.2.1]octane according to the representative procedure of Example 26, Steps 1-3 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(4-(8-oxa-3-azabicyclo[3.2.1]octan-3-yl)-2-fluorobenzyl)piperazine-1-carboxylate as an oil: 1H NMR (400 MHz, Chloroform-d) δ 7.21-7.12 (m, 1H), 6.60-6.54 (m, 1H), 6.52-6.44 (m, 1H), 5.83-5.66 (m, 1H), 4.55-4.45 (m, 2H), 3.64-3.48 (m, 6H), 3.35-3.22 (m, 2H), 3.07-2.97 (m, 2H), 2.54-2.43 (m, 4H), 2.03-1.88 (m, 4H). LCMS (ESI, m/z): 220.1 (C13H15FNO+).
Example 28
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(4-(8-oxa-3-azabicyclo[3.2.1]octan-3-yl)-2-(trifluoromethoxy)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 4-chloro-2-(trifluoromethoxy)benzaldehyde and 8-oxa-3-azabicylco[3.2.1]octane according to the representative procedure of Example 26, Steps 1-3 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(4-(8-oxa-3-azabicyclo[3.2.1]octan-3-yl)-2-(trifluoromethoxy)benzyl)piperazine-1-carboxylate as an oil: 1H NMR (400 MHz, Chloroform-d) δ 7.35-7.23 (m, 1H), 6.77-6.68 (m, 1H), 6.64 (s, 1H), 5.85-5.70 (m, 1H), 4.56-4.48 (m, 2H), 3.63-3.47 (m, 6H), 3.37-3.26 (m, 2H), 3.10-3.00 (m, 2H), 2.53-2.40 (m, 4H), 2.07-1.87 (m, 4H). LCMS (ESI, m/z): 286.0 (C14H15F3NO2+).
Example 29
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-chloro-5-(pyrrolidin-1-ylmethyl)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 3-bromo-5-chlorobenzaldehyde and pyrrolidine according to the representative procedure of Example 7, Steps 1-3 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-chloro-5-(pyrrolidin-1-ylmethyl)benzyl)piperazine-1-carboxylate as a clear oil: 1H NMR (400 MHz, Chloroform-d) δ 7.03-6.98 (m, 2H), 6.95-6.90 (m, 1H), 5.60-5.45 (m, 1H), 3.44-3.29 (m, 6H), 3.26 (s, 2H), 2.32-2.26 (m, 4H), 2.22 (dt, J=10.6, 4.8 Hz, 4H), 1.64-1.50 (m, 4H). LCMS (ESI, m/z): 488.2 [M+H]+.
Example 30
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(8-oxa-3-azabicyclo[3.2.1]octan-3-yl)-4-chlorobenzyl)piperazine-1-carboxylate




The title compound was prepared from 8-oxa-3-azabicyclo[3.2.1]octane and 4-chloro-2-fluorobenzaldehyde according to the representative procedure of Example 1 Steps 1,2, and 4 and yielded, 1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(8-oxa-3-azabicyclo[3.2.1]octan-3-yl)-4-chlorobenzyl)piperazine-1-carboxylate as a white solid. 1H NMR (400 MHz, Chloroform-d) δ 7.38 (d, J=8.2 Hz, 1H), 7.14-7.04 (m, 2H), 5.78 (hept, J=6.2 Hz, 1H), 4.44-4.38 (m, 2H), 3.60-3.52 (m, 6H), 3.05 (d, J=10.2 Hz, 2H), 2.83 (d, J=10.9 Hz, 2H), 2.48 (dt, J=9.2, 5.1 Hz, 4H), 2.16-1.95 (m, 4H). LCMS (ESI, m/z): 516.1 [M+H]+.
Example 31
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(8-oxa-3-azabicyclo[3.2.1]octan-3-yl)-3-(trifluoromethoxy)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 2-chloro-3-(trifluoromethoxy)benzaldehyde and 8-oxa-3-azabicylco[3.2.1]octane according to the representative procedure of Example 27, Steps 1-3 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(8-oxa-3-azabicyclo[3.2.1]octan-3-yl)-3-(trifluoromethoxy)benzyl)piperazine-1-carboxylate as an oil: 1H NMR (400 MHz, Chloroform-d) δ 7.39-7.31 (m, 1H), 7.26-7.12 (m, 2H), 5.87-5.64 (m, 1H), 4.44-4.28 (m, 2H), 3.71 (s, 2H), 3.66-3.49 (m, 6H), 2.64-2.43 (m, 6H), 2.17-2.06 (m, 2H), 2.05-1.93 (m, 2H). LCMS (ESI, m/z): 566.2 [M+H]+.
Example 32
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(4-(pyrrolidin-1-yl)piperidin-1-yl)-3-(trifluoromethoxy)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 2-chloro-3-(trifluoromethoxy)benzaldehyde and 4-(pyrrolidin-1-yl)piperidine according to the representative procedure of Example 27, Steps 1-3 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(4-(pyrrolidin-1-yl)piperidin-1-yl)-3-(trifluoromethoxy)benzyl)piperazine-1-carboxylate as an oil: 1H NMR (400 MHz, Chloroform-d) δ 7.36-7.30 (m, 1H), 7.19-7.05 (m, 2H), 5.84-5.65 (m, 1H), 3.63 (s, 2H), 3.59-3.48 (m, 4H), 3.25-2.87 (m, 4H), 2.72-2.53 (m, 4H), 2.53-2.41 (m, 4H), 2.16-2.05 (m, 1H), 2.05-1.94 (m, 2H), 1.91-1.78 (m, J=4.3, 3.8 Hz, 4H), 1.73-1.51 (m, 2H). LCMS (ESI, m/z): 607.3 [M+H]+.
Example 33
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(4-carbamoylpiperidin-1-yl)-4-chlorobenzyl)piperazine-1-carboxylate




Step 1: Preparation of 1-(5-chloro-2-formylphenyl)piperidine-4-carboxamide




A sealed tube was charged with 4-chloro-2-fluorobenzaldehyde (1 g, 6.30 mmol), piperidine-4-carboxamide (0.970 g, 7.57 mmol), and K2CO3 (2.78 g, 20.2 mmol). DMA (8 mL) was added and the mixture was stirred at 140° C. for 20 h. The reaction was cooled to rt, then diluted with EtOAc (200 mL). The organic layer was washed with brine (3×) and with sat. NH4Cl (1×). The organic layers were dried over Na2SO4, filtered and concentrated to yield a solid. The solid was purified on silica gel by flash column chromatography to afford 1-(5-chloro-2-formylphenyl)piperidine-4-carboxamide as a yellow solid (703 mg, 41% yield). 1H NMR (400 MHz, DMSO-d6) δ 10.07 (s, 1H), 7.74-7.62 (m, 1H), 7.40-7.28 (m, 1H), 7.26-7.16 (m, 1H), 7.19-7.10 (m, 1H), 6.85 (s, 1H), 3.33-3.21 (m, 2H), 2.96-2.81 (m, 2H), 2.31-2.20 (m, 1H), 1.89-1.71 (m, 4H).
Step 2: Preparation of 1,1,1,3,3,3-hexafluoropropan-2-yl piperazine-1-carboxylate hydrochloride




A flask was charged with triphosgene (3.18 g, 10.7 mmol). DCM (60 mL) was added and the mixture was stirred at 0° C. for 10 min while being purged with N2. Hexafluoroisopropanol was added dropwise (5.86 g, 34.8 mmol), followed by DIEA (6.94 g, 53.7 mmol). The reaction stirred at rt for 2 h. At that point, tert-butyl piperazine-1-carboxylate was added (1 g, 5.37 mmol) and the reaction was allowed to stir at rt overnight. The reaction mixture was diluted with DCM, washed with sat. NaHCO3 (3×), dried over Na2SO4, filtered and concentrated to yield an oil which was purified on silica gel by flash column chromatography to afford 1-(tert-butyl) 4-(1,1,1,3,3,3-hexafluoropropan-2-yl) piperazine-1,4-dicarboxylate as a clear oil (1.1 g, 72% yield (1H NMR (400 MHz, Chloroform-d) δ 5.69 (s, 1H), 3.52-3.36 (m, 8H), 1.40 (s, 9H).) The oil was transferred to a 16 mL vial equipped with a magnetic stir bar and stirred in 3 mL DCM at 0° C. 4 N HCl in dioxane was added dropwise (6.82 mL, 27.294 mmol) and the reaction stirred at rt overnight. The reaction was concentrated to afford 1,1,1,3,3,3-hexafluoropropan-2-yl piperazine-1-carboxylate hydrochloride as a white solid (487 mg, 38% yield). 1H NMR (400 MHz, Chloroform-d) δ 5.77-5.59 (m, 1H), 3.50-3.33 (m, 4H), 2.85-2.68 (m, 4H), 1.77 (s, 1H).
Step 3: Preparation of 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(4-carbamoylpiperidin-1-yl)-4-chlorobenzyl)piperazine-1-carboxylate




A vial was charged with 1-(5-chloro-2-formylphenyl)piperidine-4-carboxamide (100 mg, 0.375 mmol), 1,1,1,3,3,3-hexafluoropropan-2-yl piperazine-1-carboxylate hydrochloride (130 mg, 0.412 mmol), and 4 Å molecular sieves (100 mg). DMF (4 mL) was added. The vial was purged with N2 and stirred at rt for 2 h. At that point, NaBH(OAc)3 (87 mg, 0.412 mmol) was added. The reaction was stirred at rt overnight, then was diluted with EtOAc and filtered over Celite. The organic layer was washed with sat. NaHCO3 (3×), dried over Na2SO4, filtered and concentrated to yield an oil. The oil was purified on silica gel by flash column chromatography to afford 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(4-carbamoylpiperidin-1-yl)-4-chlorobenzyl)piperazine-1-carboxylate as a pale oil (58 mg, 29% yield). 1H NMR (400 MHz, Chloroform-d) δ 7.41-7.35 (m, 1H), 7.10-7.03 (m, 2H), 5.82-5.73 (m, 1H), 5.57-5.38 (m, 2H), 3.58-3.52 (m, 6H), 3.30-3.21 (m, 2H), 2.73-2.64 (m, 2H), 2.53-2.45 (m, 4H), 2.35-2.25 (m, 1H), 2.01-1.87 (m, 4H). LCMS (ESI, m/z): 531.1 [M+H]+.
Example 34
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(4-chloro-2-(4-(dimethylcarbamoyl)piperidin-1-yl)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 4-chloro-2-fluorobenzaldehyde and N,N-dimethylpiperidine-4-carboxamide according to the representative procedure of Example 33, Steps 1-3 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(4-chloro-2-(4-(dimethylcarbamoyl)piperidin-1-yl)benzyl)piperazine-1-carboxylate as a pale oil: 1H NMR (400 MHz, Chloroform-d) δ 7.40-7.32 (m, 1H), 7.07-6.99 (m, 2H), 5.82-5.70 (m, 1H), 3.60-3.46 (m, 6H), 3.33-3.21 (m, 2H), 3.10 (s, 3H), 2.99 (s, 3H), 2.75-2.61 (m, 3H), 2.54-2.42 (m, 4H), 2.07-1.93 (m, 2H), 1.87-1.74 (m, 2H). LCMS (ESI, m/z): 559.2 [M+H]+.
Example 35
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(4-(dimethylcarbamoyl)piperidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 2-fluoro-4-(trifluoromethyl)benzaldehyde and N,N-dimethylpiperidine-4-carboxamide according to the representative procedure of Example 33, Steps 1-3 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(4-(dimethylcarbamoyl)piperidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate as a pale oil: 1H NMR (400 MHz, Chloroform-d) δ 7.64-7.56 (m, 1H), 7.35-7.30 (m, 2H), 5.84-5.70 (m, 1H), 3.63 (s, 2H), 3.60-3.51 (m, 4H), 3.32-3.22 (m, 2H), 3.12 (s, 3H), 3.00 (s, 3H), 2.81-2.63 (m, 3H), 2.56-2.43 (m, 4H), 2.11-1.97 (m, 2H). LCMS (ESI, m/z): 593.2 [M+H]+.
Example 36
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(4-carbamoylpiperidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 2-fluoro-4-(trifluoromethyl)benzaldehyde and piperidine-4-carboxamide according to the representative procedure of Example 33, Steps 1-3 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(4-carbamoylpiperidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate as a clear oil: 1H NMR (400 MHz, Chloroform-d) δ 7.61 (s, 1H), 7.34 (s, 1H), 7.32-7.29 (m, 1H), 5.84-5.72 (m, 1H), 5.67-5.50 (m, 2H), 3.70-3.50 (m, 6H), 3.32-3.20 (m, 2H), 2.79-2.66 (m, 2H), 2.61-2.44 (m, 4H), 2.39-2.27 (m, 1H), 2.06-1.86 (m, 4H). LCMS (ESI, m/z): 565.2 [M+H]+.
Example 37
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(4-chloro-2-(4-(pyrrolidine-1-carbonyl)piperidin-1-yl)benzyl)piperazine-1-carboxylate




Step 1: Preparation of 4-chloro-2-[4-[(pyrrolidin-1-yl)carbonyl]piperidin-1-yl]benzaldehyde




A flask was charged with 4-chloro-2-fluorobenzaldehyde (1.00 g, 6.31 mmol, 1.00 equiv), 4-[(pyrrolidin-1-yl)carbonyl]piperidine hydrochloride (1.65 g, 7.54 mmol, 1.20 equiv), K2CO3 (3.47 g, 25.1 mmol, 3.98 equiv), DMSO (10 mL) under nitrogen. The resulting solution was stirred overnight at 100° C. and then diluted with H2O (10 mL). The resulting solution was extracted with EtOAc (3×20 mL) and the organic layers were combined, washed with H2O (3×5 mL), dried over anhydrous Na2SO4, filtered and concentrated. The residue was chromatographed on a silica gel column (1:3 EtOAc/petroleum ether) to provide of 4-chloro-2-[4-[(pyrrolidin-1-yl)carbonyl]piperidin-1-yl]benzaldehyde (1.50 g, 74% yield) as a yellow solid. 1H NMR (300 MHz, Chloroform-d) δ 10.26 (s, 1H), 7.70-7.75 (m, 1H), 7.05-7.08 (m, 2H), 3.38-3.53 (m, 6H), 2.90-2.99 (m, 2H), 1.86-2.16 (m, 8H). LCMS (ESI, m/z): 321 [M+H]+.
Step 2: Preparation of tert-butyl 4-[(4-chloro-2-[4-[(pyrrolidin-1-yl)carbonyl]piperidin-1-yl]phenyl)methyl]piperazine-1-carboxylate




A flask was charged with 4-chloro-2-[4-[(pyrrolidin-1-yl)carbonyl]piperidin-1-yl]benzaldehyde (321 mg, 1.00 mmol, 1.10 equiv), tert-butyl piperazine-1-carboxylate (169 mg, 0.909 mmol, 1.00 equiv), and 1,2-dichloroethane (10 mL). The mixture was stirred at rt for 30 min. NaBH(OAc)3 (385 mg, 1.82 mmol, 2.00 equiv) was added. The resulting solution was stirred overnight at rt and then diluted with H2O (10 mL). The resulting solution was extracted with EtOAc (3×20 mL) and the organic layers were combined, washed with H2O (3×5 mL), dried over anhydrous Na2SO4, filtered and concentrated. The residue was chromatographed on a silica gel column (1:4 EtOAc/petroleum ether) to provide of tert-butyl 4-[(4-chloro-2-[4-[(pyrrolidin-1-yl)carbonyl]piperidin-1-yl]phenyl)methyl]piperazine-1-carboxylate (400 mg, 81% yield) as light yellow oil. 1H NMR (300 MHz, Chloroform-d) δ 7.37 (d, J=8.4 Hz, 1H), 7.01-7.03 (m, 2H), 3.73 (m, 2H), 3.52-3.73 (m, 6H), 3.38-3.49 (m, 4H), 3.26-3.29 (m, 2H), 2.43-2.50 (m, 5H), 1.82-2.04 (m, 8H), 1.45 (s, 9H). LCMS (ESI, m/z): 491 [M+H]+.
Step 3: Preparation of 1-[(4-chloro-2-[4-[(pyrrolidin-1-yl)carbonyl]piperidin-1-yl]phenyl)methyl]piperazine




A flask was charged with tert-butyl 4-[(4-chloro-2-[4-[(pyrrolidin-1-yl)carbonyl]piperidin-1-yl]phenyl)methyl]piperazine-1-carboxylate (400 mg, 0.81 mmol, 1.00 equiv) and DCM (5 mL). TFA (1 mL) was added dropwise. The resulting solution was stirred overnight at rt. The resulting solution was concentrated to provide 1-[(4-chloro-2-[4-[(pyrrolidin-1-yl)carbonyl]piperidin-1-yl]phenyl)methyl]piperazine (1000 mg) as yellow oil, which was carried on without further purification. LCMS (ESI, m/z): 391 [M+H]+.
Step 4: Preparation of 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(4-chloro-2-(4-(pyrrolidine-1-carbonyl)piperidin-1-yl)benzyl)piperazine-1-carboxylate




A flask was charged with 1,1,1,3,3,3-hexafluoropropan-2-ol (215 mg, 1.28 mmol, 1.00 equiv), triphosgene (127 mg, 0.428 mmol, 0.33 equiv), and DCM (10 mL). DIEA (495 mg, 3.83 mmol, 2.99 equiv) was added dropwise. The mixture was stirred at rt for 2 h. 1-[(4-Chloro-2-[4-[(pyrrolidin-1-yl)carbonyl]piperidin-1-yl]phenyl)methyl]piperazine (500 mg, 1.28 mmol, 1.00 equiv) was added. The resulting solution was stirred for 2 h at rt and diluted with H2O (20 mL). The mixture was extracted with DCM (3×30 mL) and the organic layers were combined, washed with H2O (3×10 mL), dried over anhydrous Na2SO4, filtered and concentrated. The residue was chromatographed on a silica gel column (9:1 EtOAc/petroleum ether). The crude product (305 mg) was purified by preparative HPLC using the following gradient conditions: 20% CH3CN/80% Phase A increasing to 80% CH3CN over 10 min, then to 100% CH3CN over 0.1 min, holding at 100% CH3CN for 1.9 min, then reducing to 20% CH3CN over 0.1 min, and holding at 20% for 1.9 min, on a Waters 2767-5 Chromatograph. Column: Xbridge Prep C18, 19*150 mm 5 um; Mobile phase: Phase A: aqueous NH4HCO3 (0.05%); Phase B: CH3CN; Detector, UV220 & 254 nm. Purification resulted in yielding 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(4-chloro-2-(4-(pyrrolidine-1-carbonyl)piperidin-1-yl)benzyl)piperazine-1-carboxylate (131.3 mg, 17% yield) as an orange semi-solid. 1H NMR (300 MHz, Chloroform-d) δ 7.31-7.36 (m, 1H), 7.01-7.04 (m, 2H), 5.73-5.81 (m, 1H), 3.50-3.54 (m, 10OH), 3.24-3.28 (m, 2H), 2.63-2.70 (m, 2H), 2.50 (br, 5H), 1.97-2.07 (m, 4H), 1.79-1.92 (m, 4H). LCMS (ESI, m/z): 585 [M+H]+.
Example 38
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(4-chloro-2-(3-(3,5-dimethyl-1H-pyrazol-1-yl)azetidin-1-yl)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 4-chloro-2-fluorobenzaldehyde and 1-(azetidin-3-yl)-3,5-dimethyl-1H-pyrazole according to the representative procedure of Example 37, Steps 1-3, substituting DIEA for K2CO3 in Step 1, to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(4-chloro-2-(3-(3,5-dimethyl-1H-pyrazol-1-yl)azetidin-1-yl)benzyl)piperazine-1-carboxylate as colorless oil. 1H NMR (300 MHz, Chloroform-d): δ 7.09 (d, J=8.1 Hz, 1H), 6.75 (d, J=7.5 Hz, 1H), 6.52 (s, 1H), 5.70-5.82 (m, 2H), 5.02-5.11 (m, 1H), 4.33-4.47 (m, 4H), 3.55 (br, 4H), 3.40 (br, 2H), 2.43 (br, 4H), 2.26 (s, 3H), 2.22 (s, 3H). LCMS (ESI, m/z): 554 [M+H]+.
Example 39
1,1,1,3,3,3-hexafluoropropan-2-yl (R)-4-(4-chloro-2-(3-(methylsulfonamido)pyrrolidin-1-yl)benzyl)piperazine-1-carboxylate




Step 1: Preparation of tert-butyl N-[(3R)-1-(5-chloro-2-formylphenyl)pyrrolidin-3-yl]carbamate




A flask was charged with 4-chloro-2-fluorobenzaldehyde (2.00 g, 12.6 mmol, 1.00 equiv), tert-butyl N-[(3R)-pyrrolidin-3-yl]carbamate (2.10 g, 11.3 mmol, 0.89 equiv), K2CO3 (4.00 g, 28.9 mmol, 2.29 equiv), and DMSO (20 mL). The resulting solution was stirred overnight at 90° C. and then diluted with H2O (30 mL). The resulting solution was extracted with DCM (3×20 mL) and the organic layers were combined, dried over anhydrous Na2SO4, filtered and concentrated. The residue was chromatographed on a silica gel column (17:83 EtOAc/petroleum ether) to provide tert-butyl N-[(3R)-1-(5-chloro-2-formylphenyl)pyrrolidin-3-yl]carbamate (3.00 g, 73% yield) as a yellow solid. LCMS (ESI, m/z): 325 [M+H]+.
Step 2: Preparation of 2-[(3R)-3-aminopyrrolidin-1-yl]-5-chlorobenzaldehyde




A flask was charged with tert-butyl N-[(3R)-1-(4-chloro-2-formylphenyl)pyrrolidin-3-yl]carbamate (1.40 g, 4.31 mmol, 1.00 equiv), HCl (5 mL), and MeOH (20 mL). The resulting solution was stirred for 4 h at rt. The resulting mixture was concentrated to provide 2-[(3R)-3-aminopyrrolidin-1-yl]-5-chlorobenzaldehyde (960 mg, 99% yield) as yellow oil, which was carried on without further purification. LCMS (ESI, m/z): 225 [M+H]+.
Step 3: Preparation of N-[(3R)-1-(5-chloro-2-formylphenyl)pyrrolidin-3-yl]methanesulfonamide




A vial was charged with 2-[(3R)-3-aminopyrrolidin-1-yl]-4-chlorobenzaldehyde (150 mg, 0.670 mmol, 1.00 equiv), methanesulfonyl chloride (100 mg, 0.880 mmol, 1.30 equiv), DCM (10 mL), and triethylamine (134 mg, 1.32 mmol, 1.98 equiv). The resulting solution was stirred for 30 min at rt then diluted with H2O (10 mL). The resulting solution was extracted with DCM (3×10 mL) and the organic layers were combined, dried over anhydrous Na2SO4, filtered and concentrated. The residue was chromatographed on a silica gel column (55:45 EtOAc/petroleum ether) to provide N-[(3R)-1-(5-chloro-2-formylphenyl)pyrrolidin-3-yl]methanesulfonamide (180 mg, 89% yield) as a yellow solid. LCMS (ESI, m/z): 303 [M+H]+.
Step 4: Preparation of tert-butyl 4-([4-chloro-2-[(3R)-3-methanesulfonamidopyrrolidin-1-yl]phenyl]methyl)piperazine-1-carboxylate




A flask was charged with N-[(3R)-1-(5-chloro-2-formylphenyl)pyrrolidin-3-yl]methanesulfonamide (180 mg, 0.590 mmol, 1.00 equiv), tert-butyl piperazine-1-carboxylate (133 mg, 0.710 mmol, 1.20 equiv), and 1,2-dichloroethane (10 mL). The resulting solution was stirred for 30 min at rt. NaBH(OAc)3 (375 mg, 1.77 mmol, 2.98 equiv) was added. The resulting solution was stirred overnight at rt and diluted with H2O (10 mL). The resulting solution was extracted with DCM (2×10 mL) and the organic layers were combined, dried over anhydrous Na2SO4, filtered and concentrated. The residue was chromatographed on a silica gel column (70:30 EtOAc/petroleum ether) to provide tert-butyl 4-([4-chloro-2-[(3R)-3-methanesulfonamidopyrrolidin-1-yl]phenyl]methyl)piperazine-1-carboxylate (250 mg, 89% yield) as yellow oil. LCMS (ESI, m/z): 473 [M+H]+.
Step 5: Preparation of N-[(3R)-1-[5-chloro-2-(piperazin-1-ylmethyl)phenyl]pyrrolidin-3-yl]methanesulfonamide




A flask was charged with tert-butyl 4-([4-chloro-6-[(3R)-3-methanesulfonamidopyrrolidin-1-yl]cyclohexa-1,5-dien-1-yl]methyl)piperazine-1-carboxylate (250 mg, 0.530 mmol, 1.00 equiv), TFA (2 mL), and DCM (10 mL). The resulting solution was stirred for 3 h at rt. The resulting mixture was concentrated to provide N-[(3R)-1-[5-chloro-2-(piperazin-1-ylmethyl)phenyl]pyrrolidin-3-yl]methanesulfonamide (180 mg, 91% yield) as yellow oil, which was carried on without further purification. LCMS (ESI, m/z): 373 [M+H]+.
Step 6: Preparation of 1,1,1,3,3,3-hexafluoropropan-2-yl (R)-4-(4-chloro-2-(3-(methylsulfonamido)pyrrolidin-1-yl)benzyl)piperazine-1-carboxylate




A flask was charged with triphosgene (21.0 mg, 0.0710 mmol, 0.30 equiv), DCM (10 mL), 1,1,1,3,3,3-hexafluoropropan-2-ol (50.0 mg, 0.300 mmol, 1.00 equiv), and DIEA (62.0 mg, 0.480 mmol, 1.61 equiv). The resulting solution was stirred for 2 h at rt. N-[(3R)-1-[4-Chloro-2-(piperazin-1-ylmethyl)phenyl]pyrrolidin-3-yl]methanesulfonamide (90.0 mg, 0.240 mmol, 0.81 equiv) was added dropwise. The resulting solution was stirred overnight at rt and concentrated. The crude product (300 mg) was purified by preparative HPLC using the following gradient conditions: 20% CH3CN/80% Phase A increasing to 80% CH3CN over 10 min, then to 100% CH3CN over 0.1 min, holding at 100% CH3CN for 1.9 min, then reducing to 20% CH3CN over 0.1 min, and holding at 20% for 1.9 min, on a Waters 2767-5 Chromatograph. Column: Xbridge Prep C18, 19*150 mm 5 um; Mobile phase: Phase A: aqueous NH4HCO3 (0.05%); Phase B: CH3CN; Detector, UV220 & 254 nm. Purification resulted in 1,1,1,3,3,3-hexafluoropropan-2-yl (R)-4-(4-chloro-2-(3-(methylsulfonamido)pyrrolidin-1-yl)benzyl)piperazine-1-carboxylate (67.9 mg, 40% yield) as a white solid. 1H NMR (300 MHz, Chloroform-d) δ 7.26 (d, J=7.8 Hz, 1H), 6.90 (t, J=8.7 Hz, 2H), 5.70-5.79 (m, 1H), 4.80 (br, 1H), 4.06-4.14 (m, 1H), 3.31-3.56 (m, 9H), 3.07-3.15 (m, 1H), 3.01 (s, 3H), 2.31-2.45 (m, 5H), 1.95-1.97 (m, 1H). LCMS (ESI, m/z): 567 [M+H]+.
Example 40
1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(4-chloro-2-(3-(methylsulfonamido)pyrrolidin-1-yl)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 4-chloro-2-fluorobenzaldehyde and tert-butyl N-[(3S)-pyrrolidin-3-yl]carbamate according to the representative procedure of Example 39, Steps 1-6, to provide 1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(4-chloro-2-(3-(methylsulfonamido)pyrrolidin-1-yl)benzyl)piperazine-1-carboxylate (60.4 mg, 57% yield) as a white solid. 1H NMR (300 MHz, Chloroform-d) δ 7.26 (d, J=8.1 Hz, 1H), 6.90 (t, J=8.6 Hz, 2H), 5.70-5.79 (m, 1H), 4.80 (br, 1H), 4.06-4.14 (m, 1H), 3.30-3.56 (m, 9H), 3.07-3.15 (m, 1H), 3.01 (s, 3H), 2.31-2.45 (m, 5H), 1.95-1.97 (m, 1H). LCMS (ESI, m/z): 567 [M+H]+.
Example 41
1,1,1,3,3,3-hexafluoropropan-2-yl (R)-4-(4-chloro-2-(2-oxo-[1,3′-bipyrrolidin]-1′-yl)benzyl)piperazine-1-carboxylate




Step 1: Preparation of 4-chloro-N-[(3R)-1-(5-chloro-2-formylphenyl)pyrrolidin-3-yl]butanamide




A vial was charged with 2-[(3R)-3-aminopyrrolidin-1-yl]-4-chlorobenzaldehyde (50.0 mg, 0.220 mmol, 1.00 equiv) [synthesized from Example 39, Steps 1-2], 4-chlorobutanoyl chloride (39.0 mg, 0.280 mmol, 1.24 equiv), DCM (5 mL), and triethylamine (45.0 mg, 0.446 mmol, 2.00 equiv). The resulting solution was stirred for 30 min at rt and then diluted with H2O (10 mL). The mixture was extracted with DCM (3×10 mL) and the organic layers were combined, dried over anhydrous Na2SO4, filtered and concentrated. The residue was chromatographed on a silica gel column (70:30 EtOAc/petroleum ether) to provide 4-chloro-N-[(3R)-1-(5-chloro-2-formylphenyl)pyrrolidin-3-yl]butanamide (60.0 mg, 82% yield) as yellow oil. LCMS (ESI, m/z): 329 [M+H]+.
Step 2: Preparation of tert-butyl 4-([4-chloro-2-[(3R)-3-(4-chlorobutanamido)pyrrolidin-1-yl]phenyl]methyl)pierazine-1-carboxylate




The title compound was synthesized from 4-chloro-N-[(3R)-1-(5-chloro-2-formylphenyl)pyrrolidin-3-yl]butanamide according to the representative procedure of Example 39, Step 4, to provide tert-butyl 4-([4-chloro-2-[(3R)-3-(4-chlorobutanamido)pyrrolidin-1-yl]phenyl]methyl)piperazine-1-carboxylate (90.0 mg, 56% yield) as yellow oil. LCMS (ESI, m/z): 499 [M+H]+.
Step 3: Preparation of tert-butyl 4-([4-chloro-2-[(3R)-3-(2-oxopyrrolidin-1-yl)pyrrolidin-1-yl]phenyl]methyl)piperazine-1-carboxylate




A vial was charged with tert-butyl 4-([4-chloro-2-[(3R)-3-(4-chlorobutanamido)pyrrolidin-1-yl]phenyl]methyl)piperazine-1-carboxylate (90.0 mg, 0.180 mmol, 1.00 equiv), NaH (5.30 mg, 0.220 mmol, 1.23 equiv), and DMF (2 mL). The resulting solution was stirred for 3 h at rt and diluted with H2O (5 mL). The resulting solution was extracted with DCM (3×15 mL) and the organic layers were combined, dried over anhydrous Na2SO4, filtered and concentrated. The residue was chromatographed on a silica gel column (80:20 DCM/MeOH) to provide tert-butyl 4-([4-chloro-2-[(3R)-3-(2-oxopyrrolidin-1-yl)pyrrolidin-1-yl]phenyl]methyl)piperazine-1-carboxylate (80.0 mg, 96% yield) as yellow oil. LCMS (ESI, m/z): 463 [M+H]+.
Step 4: Preparation of 1-[(3R)-1-[5-chloro-2-(piperazin-1-ylmethyl)phenyl]pyrrolidin-3-yl]pyrrolidin-2-one




The title compound was synthesized from tert-butyl 4-([4-chloro-2-[(3R)-3-(2-oxopyrrolidin-1-yl)pyrrolidin-1-yl]phenyl]methyl)piperazine-1-carboxylate according to the representative procedure of 39, Step 5, to provide 1-[(3R)-1-[5-chloro-2-(piperazin-1-ylmethyl)phenyl]pyrrolidin-3-yl]pyrrolidin-2-one (70.0 mg) as yellow oil, which was carried on without further purification. LCMS (ESI, m/z): 363 [M+H]+.
Step 5: Preparation of 1,1,1,3,3,3-hexafluoropropan-2-yl (R)-4-(4-chloro-2-(2-oxo-[1,3′-bipyrrolidin]-1′-yl)benzyl)piperazine-1-carboxylate




The title compound was synthesized from 1-[(3R)-1-[5-chloro-2-(piperazin-1-ylmethyl)phenyl]pyrrolidin-3-yl]pyrrolidin-2-one according to the representative procedure of 39, Step 6, to provide 1,1,1,3,3,3-hexafluoropropan-2-yl (R)-4-(4-chloro-2-(2-oxo-[1,3′-bipyrrolidin]-1′-yl)benzyl)piperazine-1-carboxylate as a white solid. 1H NMR (300 MHz, Chloroform-d) δ 7.31 (d, J=7.8 Hz, 1H), 6.88-6.92 (m, 2H), 5.70-5.79 (m, 1H), 4.85-4.86 (m, 1H), 3.44-3.55 (m, 8H), 3.21-3.37 (m, 2H), 3.10-3.19 (m, 2H), 2.35-2.51 (m, 6H), 2.19-2.28 (m, 1H), 1.93-2.09 (m, 3H). LCMS (ESI, m/z): 557 [M+H]+.
Example 42
1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(4-chloro-2-(2-oxo-[1,3′-bipyrrolidin]-1′-yl)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 2-[(3S)-3-aminopyrrolidin-1-yl]-4-chlorobenzaldehyde according to the representative procedure of 41 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(4-chloro-2-(2-oxo-[1,3′-bipyrrolidin]-1′-yl)benzyl)piperazine-1-carboxylate as yellow oil. 1H NMR (300 MHz, Chloroform-d) δ 7.27-7.29 (m, 1H), 6.87-6.92 (m, 2H), 5.71-5.79 (m, 1H), 4.85-4.89 (m, 1H), 3.49-3.54 (m, 8H), 3.35-3.47 (m, 2H), 3.11-3.32 (m, 2H), 2.27-2.44 (m, 6H), 2.19-2.26 (m, 1H), 1.95-2.09 (m, 3H). LCMS (ESI, m/z): 579 [M+Na]+.
Example 43
1,1,1,3,3,3-hexafluoropropan-2-yl (R)-4-(4-chloro-2-(3-(2-oxopiperidin-1-yl)pyrrolidin-1-yl)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 5-bromopentanoyl chloride according to the representative procedure of 41, Steps 1-5, to provide 1,1,1,3,3,3-hexafluoropropan-2-yl (R)-4-(4-chloro-2-(3-(2-oxopiperidin-1-yl)pyrrolidin-1-yl)benzyl)piperazine-1-carboxylate as yellow oil. 1H NMR (300 MHz, Chloroform-d) δ 7.26-7.29 (m, 1H), 6.88-6.92 (m, 2H), 5.71-5.79 (m, 1H), 5.36-5.41 (m, 1H), 3.44-3.54 (m, 6H), 3.19-3.37 (m, 4H), 3.09-3.18 (m, 2H), 2.42-2.44 (m, 6H), 2.17-2.28 (m, 1H), 1.98-2.05 (m, 1H), 1.80-1.95 (m, 4H). LCMS (ESI, m/z): 593 [M+Na]+.
Example 44
1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(4-chloro-2-(3-(2-oxopiperidin-1-yl)pyrrolidin-1-yl)benzyl)piperazine-1-carboxylate




The title compound was synthesized according to the representative procedure of 43 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(4-chloro-2-(3-(2-oxopiperidin-1-yl)pyrrolidin-1-yl)benzyl)piperazine-1-carboxylate as yellow oil. 1H NMR (300 MHz, Chloroform-d) δ 7.26-7.29 (m, 1H), 6.89-6.92 (m, 2H), 5.71-5.81 (m, 1H), 5.37-5.41 (m, 1H), 3.44-3.54 (m, 6H), 3.21-3.37 (m, 4H), 3.09-3.19 (m, 2H), 2.44 (br, 6H), 2.20-2.28 (m, 1H), 1.93-2.18 (m, 1H), 1.75-1.88 (m, 4H). LCMS (ESI, m/z): 593 [M+Na]+.
Example 45
1,1,1,3,3,3-hexafluoropropan-2-yl (R)-4-(4-chloro-2-(3-(1,1-dioxidoisothiazolidin-2-yl)pyrrolidin-1-yl)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 3-chloropropane-1-sulfonyl chloride according to the representative procedure of 41, Steps 1-5, to provide 1,1,1,3,3,3-hexafluoropropan-2-yl (R)-4-(4-chloro-2-(3-(1,1-dioxidoisothiazolidin-2-yl)pyrrolidin-1-yl)benzyl)piperazine-1-carboxylate as yellow oil. 1H NMR (300 MHz, Chloroform-d) δ 7.21-7.25 (m, 1H), 6.84-6.88 (m 2H), 5.70-5.79 (m, 1H), 4.02-4.11 (m, 1H), 3.42-3.64 (m, 8H), 3.24-3.40 (m, 3H), 3.17-3.22 (m, 3H), 2.26-2.45 (m, 7H), 2.08-2.18 (m, 1H). LCMS (ESI, m/z): 593 [M+H]+.
Example 46
1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(4-chloro-2-(3-(1,1-dioxidoisothiazolidin-2-yl)pyrrolidin-1-yl)benzyl)piperazine-1-carboxylate




The title compound was synthesized according to the representative procedure of 45 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(4-chloro-2-(3-(1,1-dioxidoisothiazolidin-2-yl)pyrrolidin-1-yl)benzyl)piperazine-1-carboxylate as yellow oil. 1H NMR (300 MHz, Chloroform-d) δ 7.22-7.26 (m, 1H), 6.84-6.91 (m, 2H), 5.70-5.78 (m, 1H), 4.02-4.09 (m, 1H), 3.41-3.64 (m, 8H), 3.22-3.39 (m, 3H), 3.12-3.19 (m, 3H), 2.26-2.44 (m, 7H), 2.06-2.18 (m, 1H). LCMS (ESI, m/z): 593 [M+H]+.
Example 47
1,1,1,3,3,3-hexafluoropropan-2-yl (R)-4-(2-(3-(methylsulfonamido)pyrrolidin-1-yl)-6-(trifluoromethyl)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 2-fluoro-6-(trifluoromethyl)benzaldehyde according to the representative procedure of 39 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl (R)-4-(2-(3-(methylsulfonamido)pyrrolidin-1-yl)-6-(trifluoromethyl)benzyl)piperazine-1-carboxylate as a white solid. 1H NMR (300 MHz, Chloroform-d) δ 7.31-7.41 (m, 2H), 7.22-7.25 (m, 1H), 5.70-5.78 (m, 1H), 4.94-4.96 (m, 1H), 4.15 (br, 1H), 3.71-3.82 (m, 2H), 3.40-3.42 (m, 4H), 3.32-3.38 (m, 2H), 3.13-3.17 (m, 1H), 3.00-3.10 (m, 4H), 2.37-2.46 (m, 5H), 1.98-2.05 (m, 1H). LCMS (ESI, m/z): 601 [M+H]+.
Example 48
1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(2-(3-(methylsulfonamido)pyrrolidin-1-yl)-6-(trifluoromethyl)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available N-[(3S)-pyrrolidin-3-yl]carbamate according to the representative procedure of 47 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(2-(3-(methylsulfonamido)pyrrolidin-1-yl)-6-(trifluoromethyl)benzyl)piperazine-1-carboxylate as yellow oil. 1H NMR (300 MHz, Chloroform-d) δ 7.30-7.40 (m, 2H), 7.21-7.24 (m, 1H), 5.69-5.77 (m, 1H), 4.90-4.92 (m, 1H), 4.13-4.14 (m, 1H), 3.70-3.81 (m, 2H), 3.49-3.50 (m, 4H), 3.31-3.40 (m, 2H), 3.01-3.16 (m, 5H), 2.36-2.45 (m, 5H), 2.00 (br, 1H). LCMS (ESI, m/z): 601 [M+H]+.
Example 49
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(3-oxa-8-azabicyclo[3.2.1]octan-8-yl)-6-(trifluoromethyl)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 2-fluoro-6-trifluoromethylbenzaldehyde and 3-oxa-8-azabicyclo[3.2.1]octane according to the representative procedure of 37 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(3-oxa-8-azabicyclo[3.2.1]octan-8-yl)-6-(trifluoromethyl)benzyl)piperazine-1-carboxylate as yellow oil. 1H NMR (300 MHz, Chloroform-d) δ 7.35 (d, J=7.2 Hz, 1H), 7.24-7.29 (m, 1H), 7.05 (d, J=7.8 Hz, 1H), 5.69-5.82 (m, 1H), 3.96 (d, J=10.2 Hz, 2H), 3.87 (s, 2H), 3.67 (d, J=10.2 Hz, 2H), 3.45-3.51 (m, 6H), 2.42-2.46 (m, 4H), 1.93-2.09 (m, 4H). LCMS (ESI, m/z): 550 [M+H]+.
Example 50
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(8-oxa-3-azabicyclo[3.2.1]octan-3-yl)-6-(trifluoromethyl)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 2-fluoro-6-(trifluoromethyl)benzaldehyde and 8-oxa-3-azabicyclo[3.2.1]octane according to the representative procedure of 37 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(8-oxa-3-azabicyclo[3.2.1]octan-3-yl)-6-(trifluoromethyl)benzyl)piperazine-1-carboxylate as a white solid. 1H NMR (300 MHz, Chloroform-d) δ 7.35-7.51 (m, 3H), 5.68-5.81 (m, 1H), 4.41 (s, 2H), 3.86 (s, 2H), 3.47-3.48 (m, 4H), 3.09-3.13 (m, 2H), 2.65 (d, J=11.1 Hz, 2H), 2.39-2.40 (m, 4H), 1.96-2.18 (m, 4H). LCMS (ESI, m/z): 550 [M+H]+.
Example 51
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-methyl-2-(4-(methylsulfonamido)piperidin-1-yl)benzyl)piperazine-1-carboxylate




Step 1: Preparation of (2-bromo-3-methylphenyl)methanol




A flask was charged with methyl 2-bromo-3-methylbenzoate (10.0 g, 43.6 mmol, 1.00 equiv), lithium borohydride (4.80 g, 231 mmol, 5.00 equiv), and THF (100 mL). The resulting solution was stirred for 4 h at 50° C. and then diluted with H2O (30 mL). The resulting mixture was extracted with EtOAc (3×50 mL) and the organic layers were combined, washed with brine (1×200 mL), dried over anhydrous Na2SO4, filtered and concentrated. The residue was chromatographed on a silica gel column (30:70 EtOAc/petroleum ether) to provide (2-bromo-3-methylphenyl)methanol (7.50 g, 85% yield) as a white solid. GCMS (EI, m/z): 200 [M]+.
Step 2: Preparation of 2-bromo-3-methylbenzaldehyde




A flask was charged with (2-bromo-3-methylphenyl)methanol (5.00 g, 24.9 mmol, 1.00 equiv), pyridinium chlorochromate (16.2 g, 75.0 mmol, 3.00 equiv), and DCM (70 mL). The resulting solution was stirred overnight at rt and concentrated. The residue was chromatographed on a silica gel column (20:80 EtOAc/petroleum ether) to provide 2-bromo-3-methylbenzaldehyde (4.40 g, 89% yield) as a white solid. GCMS (EI, m/z): 198 [M]+.
Step 3: Preparation of tert-butyl 4-[(2-bromo-3-methylphenyl)methyl]piperazine-1-carboxylate




A flask was charged with 2-bromo-3-methylbenzaldehyde (3.00 g, 15.1 mmol, 1.00 equiv), tert-butyl piperazine-1-carboxylate (3.40 g, 18.2 mmol, 1.20 equiv), and 1,2-dichloroethane (50 mL). The mixture was stirred for 30 min at rt. NaBH(OAc)3 (9.60 g, 45.3 mmol, 3.00 equiv) was added. The resulting solution was stirred overnight at rt and then diluted with H2O (20 mL). The resulting mixture was extracted with DCM (3×20 mL) and the organic layers were combined, washed with brine (1×100 mL), dried over anhydrous Na2SO4, filtered and concentrated. The residue was chromatographed on a silica gel column (20:80 EtOAc/petroleum ether) to provide tert-butyl 4-[(2-bromo-3-methylphenyl)methyl]piperazine-1-carboxylate (4.80 g, 86% yield) as colorless oil. LCMS (ESI, m/z): 369 [M+H]+.
Step 4: Preparation of tert-butyl 4-[[2-(4-methanesulfonamidopiperidin-1-yl)-3-methylphenyl]methyl]piperazine-1-carboxylate




A flask was charged with tert-butyl 4-[(2-bromo-3-methylphenyl)methyl]piperazine-1-carboxylate (500 mg, 1.35 mmol, 1.00 equiv), N-(piperidin-4-yl)methanesulfonamide (363 mg, 2.04 mmol, 1.50 equiv), tris(dibenzylideneacetone)dipalladium (62.0 mg, 0.0700 mmol, 0.05 equiv), 2-(diphenylphosphino)-1-(2-(diphenylphosphino)naphthalen-1-yl)naphthalene (127 mg, 0.200 mmol, 0.15 equiv), sodium tert-butoxide (196 mg, 2.04 mmol, 1.50 equiv), and toluene (20 mL) under nitrogen. The resulting solution was stirred overnight at 100° C. and concentrated. The residue was chromatographed on a silica gel column (30:70 EtOAc/petroleum ether) to provide tert-butyl 4-[[2-(4-methanesulfonamidopiperidin-1-yl)-3-methylphenyl]methyl]piperazine-1-carboxylate (210 mg, 33% yield) as yellow oil. LCMS (ESI, m/z): 467 [M+H]+.
Step 5: Preparation of N-[1-[2-methyl-6-(piperazin-1-ylmethyl)phenyl]piperidin-4-yl]methanesulfonamide




A flask was charged with tert-butyl 4-[[2-(4-methanesulfonamidopiperidin-1-yl)-3-methylphenyl]methyl]piperazine-1-carboxylate (210 mg, 0.450 mmol, 1.00 equiv), TFA (2 mL), and DCM (10 mL). The resulting solution was stirred overnight at rt and concentrated to yield N-[1-[2-methyl-6-(piperazin-1-ylmethyl)phenyl]piperidin-4-yl]methanesulfonamide (150 mg, 91% yield) as yellow oil, which was carried on without further purification. LCMS (ESI, m/z): 367 [M+H]+.
Step 6: Preparation of 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-methyl-2-(4-(methylsulfonamido)piperidin-1-yl)benzyl)piperazine-1-carboxylate




A flask was charged with triphosgene (91.0 mg, 0.310 mmol, 0.70 equiv) and DCM (15 mL). 1,1,1,3,3,3-hexafluoropropan-2-ol (147 mg, 0.870 mmol, 2.00 equiv) and DIEA (451 mg, 3.49 mmol, 8.00 equiv) were added at 0° C. The mixture was stirred for 2 h at rt. N-[1-[2-methyl-6-(piperazin-1-ylmethyl)phenyl]piperidin-4-yl]methanesulfonamide (160 mg, 0.440 mmol, 1.00 equiv) was added. The resulting solution was stirred overnight at rt and concentrated. The crude product (700 mg) was purified by preparative HPLC using the following gradient conditions: 20% CH3CN/80% Phase A increasing to 80% CH3CN over 10 min, then to 100% CH3CN over 0.1 min, holding at 100% CH3CN for 1.9 min, then reducing to 20% CH3CN over 0.1 min, and holding at 20% for 1.9 min, on a Waters 2767-5 Chromatograph. Column: Xbridge Prep C18, 19*150 mm 5 um; Mobile phase: Phase A: aqueous NH4HCO3 (0.05%); Phase B: CH3CN; Detector, UV220 & 254 nm. Purification resulted in 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-methyl-2-(4-(methylsulfonamido)piperidin-1-yl)benzyl)piperazine-1-carboxylate (105.8 mg, 43% yield) as a yellow solid. 1H NMR (300 MHz, Chloroform-d) δ 6.97-7.18 (m, 3H), 5.71-5.79 (m, 1H), 4.31-4.36 (m, 1H), 3.42-3.54 (m, 7H), 3.13-3.39 (m, 2H), 2.99-3.07 (m, 5H), 2.44-2.47 (m, 4H), 2.31-2.33 (m, 3H), 2.03-2.06 (m, 2H), 1.62-1.74 (m, 2H). LCMS (ESI, m/z): 561 [M+H]+.
Example 52
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-methyl-3-(4-(methylsulfonamido)piperidin-1-yl)benzyl)piperazine-1-carboxylate




Step 1: Preparation of tert-butyl 4-[(3-bromo-2-methylphenyl)methyl]piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 3-bromo-2-methylbenzaldehyde according to the representative procedure of 51, Step 3, to provide tert-butyl 4-[(3-bromo-2-methylphenyl)methyl]piperazine-1-carboxylate (5.10 g, 92% yield) as colorless oil. LCMS (ESI, m/z): 369 [M+H]+.
Step 2: Preparation of tert-butyl 4-[[3-(4-methanesulfonamidopiperidin-1-yl)-2-methylphenyl]methyl]piperazine-1-carboxylate




The title compound was synthesized according to the representative procedure of 51, Step 4, to provide tert-butyl 4-[[3-(4-methanesulfonamidopiperidin-1-yl)-2-methylphenyl]methyl]piperazine-1-carboxylate as yellow oil. LCMS (ESI, m/z): 467 [M+H]+.
Step 3: Preparation of N-[1-[2-methyl-3-(piperazin-1-ylmethyl)phenyl]piperidin-4-yl]methanesulfonamide




The title compound was synthesized according to the representative procedure of 51, Step 5, to provide N-[1-[2-methyl-3-(piperazin-1-ylmethyl)phenyl]piperidin-4-yl]methanesulfonamide as yellow oil. LCMS (ESI, m/z): 367 [M+H]+.
Step 4: Preparation of 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-methyl-3-(4-(methylsulfonamido)piperidin-1-yl)benzyl)piperazine-1-carboxylate




The title compound was synthesized according to the representative procedure of 51, Step 6, to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-methyl-3-(4-(methylsulfonamido)piperidin-1-yl)benzyl)piperazine-1-carboxylate as a white solid. 1H NMR (300 MHz, Chloroform-d) δ 7.09-7.14 (m, 1H), 6.96-7.00 (m, 2H), 5.71-5.79 (m, 1H), 4.27-4.30 (m, 1H), 3.47-3.52 (m, 7H), 3.03-3.09 (m, 5H), 2.70-2.78 (m, 2H), 2.45 (br, 4H), 2.30 (s, 3H), 2.10-2.14 (m, 2H), 1.67-1.80 (m, 2H). LCMS (ESI, m/z): 561 [M+H]+.
Example 53
1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(2-(3-(fluoromethyl)pyrrolidin-1-yl)-4-methylbenzyl)piperazine-1-carboxylate




Step 1: Preparation of 2-[(3S)-3-(hydroxymethyl)pyrrolidin-1-yl]-4-methylbenzaldehyde




A flask was charged with 2-fluoro-4-methylbenzaldehyde (1.00 g, 7.24 mmol, 1.00 equiv), (3S)-pyrrolidin-3-ylmethanol (1.10 g, 10.9 mmol, 1.50 equiv), K2CO3 (2.00 g, 14.5 mmol, 2.00 equiv), and DMSO (10 mL). The resulting solution was stirred overnight at 120° C. and then diluted with H2O (10 mL). The resulting mixture was extracted with EtOAc (3×10 mL). The organic layers were combined, washed with brine (1×50 mL), dried over anhydrous Na2SO4, filtered and concentrated. The residue was chromatographed on a silica gel column (50:50 EtOAc/petroleum ether) to provide 2-[(3S)-3-(hydroxymethyl)pyrrolidin-1-yl]-4-methylbenzaldehyde (1.20 g, 76% yield) as yellow oil. LCMS (ESI, m/z): 220 [M+H]+.
Step 2: Preparation of tert-butyl 4-([2-[(3S)-3-(hydroxymethyl)pyrrolidin-1-yl]-4-methylphenyl]methyl)piperazine-1-carboxylate




A flask was charged with 2-[(3S)-3-(hydroxymethyl)pyrrolidin-1-yl]-4-methylbenzaldehyde (0.700 g, 3.19 mmol, 1.00 equiv), tert-butyl piperazine-1-carboxylate (0.713 g, 3.83 mmol, 1.20 equiv), and 1,2-dichloroethane (10 mL). The mixture was stirred for 1 h at rt. NaBH(OAc)3 (2.00 g, 9.44 mmol, 3.00 equiv) was added. The resulting solution was stirred overnight at rt and then diluted with H2O (10 mL). The resulting mixture was extracted with DCM (3×10 mL). The organic layers were combined, washed with brine (1×50 mL), dried over anhydrous Na2SO4, filtered and concentrated. The residue was chromatographed on a silica gel column (30:70 EtOAc/petroleum ether) to provide tert-butyl 4-([2-[(3S)-3-(hydroxymethyl)pyrrolidin-1-yl]-4-methylphenyl]methyl)piperazine-1-carboxylate (1.10 g, 88% yield) as yellow oil. LCMS (ESI, m/z): 390 [M+H]+.
Step 3: Preparation of tert-butyl 4-([4-methyl-2-[(3S)-3-([[(4-methylbenzene)sulfonyl]oxy]methyl)pyrrolidin-1-yl]phenyl]methyl)piperazine-1-carboxylate




A flask was charged with tert-butyl 4-([2-[(3S)-3-(hydroxymethyl)pyrrolidin-1-yl]-4-methylphenyl]methyl)piperazine-1-carboxylate (1.10 g, 2.82 mmol, 1.00 equiv), 4-methylbenzene-1-sulfonyl chloride (0.808 g, 4.24 mmol, 1.50 equiv), pyridine (0.446 g, 5.64 mmol, 2.00 equiv), 4-dimethylaminopyridine (0.0340 g, 0.280 mmol, 0.10 equiv), and DCM (15 mL). The resulting solution was stirred overnight at 40° C. and then diluted with H2O (10 mL). The resulting mixture was extracted with DCM (3×10 mL). The organic layers were combined and washed with brine (1×50 mL), dried over anhydrous Na2SO4, filtered and concentrated. The residue was chromatographed on a silica gel column (90:10 DCM/MeOH) to provide tert-butyl 4-([4-methyl-2-[(3 S)-3-([[(4-methylbenzene)sulfonyl]oxy]methyl)pyrrolidin-1-yl]phenyl]methyl)piperazine-1-carboxylate (1.00 g, 65% yield) as yellow oil. LCMS (ESI, m/z): 544 [M+H]+.
Step 4: Preparation of tert-butyl 4-([2-[(3S)-3-(fluoromethyl)pyrrolidin-1-yl]-4-methylphenyl]methyl)piperazine-1-carboxylate




A flask was charged with tert-butyl 4-([4-methyl-2-[(3S)-3-([[(4-methylbenzene)sulfonyl]oxy]methyl)pyrrolidin-1-yl]phenyl]methyl)piperazine-1-carboxylate (1.00 g, 1.84 mmol, 1.00 equiv), tetrabutylammonium fluoride (1.90 g, 7.27 mmol, 4.00 equiv), and THF (10 mL). The resulting solution was stirred overnight at 60° C. and concentrated. The residue was chromatographed on a silica gel column (15:85 EtOAc/petroleum ether) to provide tert-butyl 4-([2-[(3 S)-3-(fluoromethyl)pyrrolidin-1-yl]-4-methylphenyl]methyl)piperazine-1-carboxylate (0.290 g, 40% yield) as colorless oil. LCMS (ESI, m/z): 392 [M+H]+.
Step 5: Preparation of 1-([2-[(3S)-3-(fluoromethyl)pyrrolidin-1-yl]-4-methylphenyl]methyl)piperazine




The title compound was synthesized according to the representative procedure of 37, Step 3, to provide 1-([2-[(3S)-3-(fluoromethyl)pyrrolidin-1-yl]-4-methylphenyl]methyl)piperazine as yellow oil. LCMS (ESI, m/z): 292 [M+H]+.
Step 6: Preparation of 1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(2-(3-(fluoromethyl)pyrrolidin-1-yl)-4-methylbenzyl)piperazine-1-carboxylate




The title compound was synthesized according to the representative procedure of 37, Step 4, to provide 1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(2-(3-(fluoromethyl)pyrrolidin-1-yl)-4-methylbenzyl)piperazine-1-carboxylate as yellow oil. 1H NMR (300 MHz, Chloroform-d) δ 7.22-7.25 (m, 1H), 6.76-6.80 (m, 2H), 5.71-5.79 (m, 1H), 4.50 (d, J=7.2 Hz, 1H), 4.34 (d, J=6.9 Hz, 1H), 3.44-3.59 (m, 6H), 3.10-3.26 (m, 4H), 2.59-2.72 (m, 1H), 2.47 (br, 4H), 2.32 (s, 3H), 2.01-2.12 (m, 1H), 1.57-1.70 (m, 1H). LCMS (ESI, m/z): 486 [M+H]+.
Example 54
1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(2-(3-(fluoromethyl)pyrrolidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 2-fluoro-4-(trifluoromethyl)benzaldehyde according to the representative procedure of 53 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(2-(3-(fluoromethyl)pyrrolidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate as colorless oil. 1H NMR (300 MHz, Chloroform-d) δ 7.51-7.55 (m, 1H), 7.14-7.18 (m, 2H), 5.71-5.80 (m, 1H), 4.50-4.54 (m, 1H), 4.31-4.47 (m, 1H), 3.50-3.63 (m, 6H), 3.18-3.29 (m, 4H), 2.65-2.74 (m, 1H), 2.44-2.47 (m, 4H), 2.06-2.16 (m, 1H), 1.65-1.76 (m, 1H). LCMS (ESI, m/z): 540 [M+H]+.
Example 55
1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(3-chloro-2-(3-(fluoromethyl)pyrrolidin-1-yl)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 3-chloro-2-fluorobenzaldehyde according to the representative procedure of 53 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(3-chloro-2-(3-(fluoromethyl)pyrrolidin-1-yl)benzyl)piperazine-1-carboxylate as orange oil. 1H NMR (300 MHz, Chloroform-d) δ 7.28-7.30 (m, 2H), 7.07-7.12 (m, 1H), 5.71-5.80 (m, 1H), 4.54-4.57 (m, 1H), 4.38-4.41 (m, 1H), 3.52-3.63 (m, 6H), 3.26-3.46 (m, 3H), 3.08-3.12 (m, 1H), 2.74-2.79 (m, 1H), 2.44-2.46 (m, 4H), 2.10-2.16 (m, 1H), 1.76-1.83 (m, 1H). LCMS (ESI, m/z): 506 [M+H]+.
Example 56
1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(2-(3-(fluoromethyl)pyrrolidin-1-yl)-3-methylbenzyl)piperazine-1-carboxylate




Step 1: Preparation of tert-butyl 4-([2-[(3S)-3-(hydroxymethyl)pyrrolidin-1-yl]-3-methylphenyl]methyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available (3S)-pyrrolidin-3-ylmethanol according to the representative procedure of 51, Step 4, to provide tert-butyl 4-([2-[(3S)-3-(hydroxymethyl)pyrrolidin-1-yl]-3-methylphenyl]methyl)piperazine-1-carboxylate as yellow oil. LCMS (ESI, m/z): 390 [M+H]+.
Step 2: Preparation of tert-butyl 4-([3-methyl-2-[(3S)-3-([[(4-methylbenzene)sulfonyl]oxy]methyl)pyrrolidin-1-yl]phenyl]methyl)piperazine-1-carboxylate




The title compound was synthesized according to the representative procedure of 53, Step 3, to provide tert-butyl 4-([3-methyl-2-[(3S)-3-([[(4-methylbenzene)sulfonyl]oxy]methyl)pyrrolidin-1-yl]phenyl]methyl)piperazine-1-carboxylate (320 mg, 82% yield) as yellow oil. LCMS (ESI, m/z): 544 [M+H]+.
Step 3: Preparation of tert-butyl 4-([2-[(3S)-3-(fluoromethyl)pyrrolidin-1-yl]-3-methylphenyl]methyl)piperazine-1-carboxylate




The title compound was synthesized according to the representative procedure of 53, Step 4, to provide tert-butyl 4-([2-[(3S)-3-(fluoromethyl)pyrrolidin-1-yl]-3-methylphenyl]methyl)piperazine-1-carboxylate (80.0 mg, 35% yield) as colorless oil. LCMS (ESI, m/z): 392 [M+H]+.
Step 4: Preparation of 1-([2-[(3S)-3-(fluoromethyl)pyrrolidin-1-yl]-3-methylphenyl]methyl)piperazine




The title compound was synthesized according to the representative procedure of 37, Step 3, to provide 1-([2-[(3S)-3-(fluoromethyl)pyrrolidin-1-yl]-3-methylphenyl]methyl)piperazine as yellow oil. LCMS (ESI, m/z): 292 [M+H]+.
Step 5: Preparation of 1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(2-(3-(fluoromethyl)pyrrolidin-1-yl)-3-methylbenzyl)piperazine-1-carboxylate




The title compound was synthesized according to the representative procedure of 37, Step 4, to provide 1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(2-(3-(fluoromethyl)pyrrolidin-1-yl)-3-methylbenzyl)piperazine-1-carboxylate as yellow oil. 1H NMR (300 MHz, Chloroform-d) δ 7.24 (s, 1H), 7.04-7.12 (m, 2H), 5.71-5.80 (m, 1H), 4.54-4.56 (m, 1H), 4.38-4.41 (m, 1H), 3.52-3.55 (m, 6H), 3.22-3.37 (m, 3H), 3.06-3.10 (m, 1H), 2.68-2.80 (m, 1H), 2.44-2.46 (m, 4H), 2.27 (s, 3H), 2.06-2.16 (m, 1H), 1.72-1.83 (m, 1H). LCMS (ESI, m/z): 486 [M+H]+.
Example 57
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(8-oxa-3-azabicyclo[3.2.1]octan-3-yl)-3-chlorobenzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 3-chloro-2-fluorobenzaldehyde and 8-oxa-3-azabicyclo[3.2.1]octane according to the representative procedure of 37 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(8-oxa-3-azabicyclo[3.2.1]octan-3-yl)-3-chlorobenzyl)piperazine-1-carboxylate as a white solid. 1H NMR (400 MHz, Chloroform-d) 7.32-7.40 (m, 1H), 7.24-7.26 (m, 1H), 7.05-7.14 (m, 1H), 5.71-5.80 (m, 1H), 4.35-4.40 (m, 2H), 3.91-3.94 (m, 2H), 3.51-3.70 (m, 6H), 2.44-2.67 (m, 6H), 2.11-2.34 (m, 2H), 1.93-2.10 (m, 2H). LCMS (ESI, m/z): 516 [M+H]+.
Example 58
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(3-oxa-8-azabicyclo[3.2.1]octan-8-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 2-fluoro-4-(trifluoromethyl)benzaldehyde and 3-oxa-8-azabicyclo[3.2.1]octane according to the representative procedure of 37 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(3-oxa-8-azabicyclo[3.2.1]octan-8-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate as an off-white solid. 1H NMR (300 MHz, Chloroform-d) δ 7.57 (d, J=7.8 Hz, 1H), 7.22 (d, J=7.8 Hz, 1H), 7.06 (s, 1H), 5.71-5.79 (m, 1H), 3.91 (d, J=10.2 Hz, 2H), 3.64-3.72 (m, 6H), 3.56 (br, 4H), 2.52 (br, 4H), 1.94-2.07 (m, 4H). LCMS (ESI, m/z): 550 [M+H]+.
Example 59
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(8-oxa-3-azabicyclo[3.2.1]octan-3-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 2-fluoro-4-(trifluoromethyl)benzaldehyde and 8-oxa-3-azabicyclo[3.2.1]octane according to the representative procedure of 37 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(8-oxa-3-azabicyclo[3.2.1]octan-3-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate as a yellow solid. 1H NMR (300 MHz, Chloroform-d) δ 7.59 (d, J=7.8 Hz, 1H), 7.33-7.36 (m, 2H), 5.69-5.82 (m, 1H), 4.42 (s, 2H), 3.64 (s, 2H), 3.56-3.57 (m, 4H), 3.09 (d, J=10.5 Hz, 2H), 2.80 (d, J=11.1 Hz, 2H), 2.47-2.48 (m, 4H), 1.93-2.12 (m, 4H). LCMS (ESI, m/z): 550 [M+H]+.
Example 60
1,1,1,3,3,3-hexafluoropropan-2-yl (R)-4-(2-(3-(methylsulfonamido)pyrrolidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate




Step 1: Preparation of tert-butyl N-[(3R)-1-[2-formyl-5-(trifluoromethyl)phenyl]pyrrolidin-3-yl]carbamate




A flask was charged with 2-fluoro-4-(trifluoromethyl)benzaldehyde (1.00 g, 5.21 mmol, 1.00 equiv), tert-butyl N-[(3R)-pyrrolidin-3-yl]carbamate (1.16 g, 6.23 mmol, 1.20 equiv), K2CO3 (2.12 g, 15.3 mmol, 3.00 equiv), and DMSO (10 mL) under nitrogen. The resulting solution was stirred overnight at 90° C. and diluted with H2O (20 mL). The mixture was extracted with DCM (3×30 mL) and the organic layers were combined, washed with H2O (3×10 mL), dried over anhydrous Na2SO4, filtered and concentrated. The residue was chromatographed on a silica gel column (12:88 EtOAc/petroleum ether) to provide tert-butyl N-[(3R)-1-[2-formyl-5-(trifluoromethyl)phenyl]pyrrolidin-3-yl]carbamate (1.30 g, 70% yield) as a yellow solid. LCMS (ESI, m/z): 359 [M+H]+.
Step 2: Preparation of 2-[(3R)-3-aminopyrrolidin-1-yl]-4-(trifluoromethyl)benzaldehyde




A flask was charged with tert-butyl N-[(3R)-1-[2-formyl-5-(trifluoromethyl)phenyl]pyrrolidin-3-yl]carbamate (1.30 g, 3.63 mmol, 1.00 equiv), dioxane (16 mL), and HCl (4 mL). The resulting solution was stirred overnight at rt. The reaction progress was monitored by LCMS. The resulting mixture was concentrated to provide 2-[(3R)-3-aminopyrrolidin-1-yl]-4-(trifluoromethyl)benzaldehyde (1.20 g) as a yellow solid, which was carried on without further purification. LCMS (ESI, m/z): 259 [M+H]+.
Step 3: Preparation of N-[(3R)-1-[2-formyl-5-(trifluoromethyl)phenyl]pyrrolidin-3-yl]methanesulfonamide




A flask was charged with 2-[(3R)-3-aminopyrrolidin-1-yl]-4-(trifluoromethyl)benzaldehyde (600 mg, 2.32 mmol, 1.00 equiv), and DCM (5 mL). The mixture was cooled to 0° C. Triethylamine (705 mg, 6.97 mmol, 3.00 equiv) was added. Methanesulfonyl chloride (401 mg, 3.49 mmol, 1.50 equiv) was added dropwise. The resulting solution was stirred for 1 h at rt and diluted with H2O (20 mL). The mixture was extracted with DCM (3×30 mL) and the organic layers were combined, washed with H2O (3×10 mL), dried over anhydrous Na2SO4, filtered and concentrated. The residue was chromatographed on a silica gel column (2:3 EtOAc/petroleum ether) to provide N-[(3R)-1-[2-formyl-5-(trifluoromethyl)phenyl]pyrrolidin-3-yl]methanesulfonamide (290 mg, 37% yield) as yellow oil. LCMS (ESI, m/z): 337 [M+H]+.
Step 4: Preparation of tert-butyl 4-([2-[(3R)-3-methanesulfonamidopyrrolidin-1-yl]-4-(trifluoromethyl)phenyl]methyl)piperazine-1-carboxylate




The title compound was synthesized according to the representative procedure of 39, Step 4, to provide tert-butyl 4-([2-[(3R)-3-methanesulfonamidopyrrolidin-1-yl]-4-(trifluoromethyl)phenyl]methyl)piperazine-1-carboxylate as a light yellow solid. LCMS (ESI, m/z): 507 [M+H]+.
Step 5: Preparation of N-[(3R)-1-[2-(piperazin-1-ylmethyl)-5-(trifluoromethyl)phenyl]pyrrolidin-3-yl]methanesulfonamide




A flask was charged with tert-butyl 4-([2-[(3R)-3-methanesulfonamidopyrrolidin-1-yl]-4-(trifluoromethyl)phenyl]methyl)piperazine-1-carboxylate (300 mg, 0.592 mmol, 1.00 equiv) and DCM (10 mL). TFA (2 mL) was added dropwise. The resulting solution was stirred overnight at rt and concentrated to provide N-[(3R)-1-[2-(piperazin-1-ylmethyl)-5-(trifluoromethyl)phenyl]pyrrolidin-3-yl]methanesulfonamide (230 mg, 96% yield) as yellow oil, which was carried on without further purification. LCMS (ESI, m/z): 407 [M+H]+.
Step 6: Preparation of 1,1,1,3,3,3-hexafluoropropan-2-yl (R)-4-(2-(3-(methylsulfonamido)pyrrolidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate




A flask was charged with 1,1,1,3,3,3-hexafluoropropan-2-ol (190 mg, 1.13 mmol, 2.00 equiv), triphosgene (117 mg, 0.394 mmol, 0.70 equiv), and DCM (5 mL). The mixture was cooled to 0° C. DIEA (292 mg, 2.26 mmol, 4.00 equiv) was added dropwise. The mixture was stirred at rt for 2 h. N-[(3R)-1-[2-(Piperazin-1-ylmethyl)-5-(trifluoromethyl)phenyl]pyrrolidin-3-yl]methanesulfonamide (230 mg, 0.565 mmol, 1.00 equiv) was added. The resulting solution was stirred for 2 h at rt and concentrated. The crude product (125 mg) was purified by preparative HPLC using the following gradient conditions: 20% CH3CN/80% Phase A increasing to 80% CH3CN over 10 min, then to 100% CH3CN over 0.1 min, holding at 100% CH3CN for 1.9 min, then reducing to 20% CH3CN over 0.1 min, and holding at 20% for 1.9 min, on a Waters 2767-5 Chromatograph. Column: Xbridge Prep C18, 19*150 mm 5 um; Mobile phase: Phase A: aqueous NH4HCO3 (0.05%); Phase B: CH3CN; Detector, UV220 & 254 nm. Purification resulted in 1,1,1,3,3,3-hexafluoropropan-2-yl (R)-4-(2-(3-(methylsulfonamido)pyrrolidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate (46.0 mg, 14% yield) as a white solid. 1H NMR (300 MHz, Chloroform-d) δ 7.50 (d, J=8.1 Hz, 1H), 7.20 (d, J=8.4 Hz, 1H), 7.11 (s, 1H), 5.70-5.79 (m, 1H), 4.69 (d, J=6.9 Hz, 1H), 4.12-4.19 (m, 1H), 3.33-3.58 (m, 9H), 3.10-3.18 (m, 1H), 3.02 (s, 3H), 2.34-2.46 (m, 5H), 1.99 (s, 1H). LCMS (ESI, m/z): 601 [M+H]+.
Example 61
1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(2-(3-(methylsulfonamido)pyrrolidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available tert-butyl N-[(3S)-pyrrolidin-3-yl]carbamate according to the representative procedure of 60 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl (S)-4-(2-(3-(methylsulfonamido)pyrrolidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate as an off-white solid. 1H NMR (300 MHz, Chloroform-d) δ 7.49 (d, J=7.8 Hz, 1H), 7.20 (d, J=7.2 Hz, 1H), 7.11 (s, 1H), 5.68-5.78 (m, 1H), 4.71 (d, J=7.5 Hz, 1H), 4.15 (s, 1H), 3.25-3.79 (m, 9H), 3.10-3.18 (m, 1H) 3.02 (s, 3H), 2.32-2.47 (m, 5H), 1.99 (s, 1H). LCMS (ESI, m/z): 601 [M+H]+.
Example 62
1-(5-chloro-2-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)phenyl)piperidine-4-carboxylic acid




Step 1: Preparation of ethyl 1-(5-chloro-2-formylphenyl) piperidine-4-carboxylate




The title compound was synthesized directly from commercially available 2-fluoro-4-chlorobenzaldehyde according to the representative procedure of 60, Step 1, to provide ethyl 1-(5-chloro-2-formylphenyl) piperidine-4-carboxylate as yellow oil. LCMS (ESI, m/z): 296 [M+H]+.
Step 2: Preparation of tert-butyl 4-([4-chloro-2-[4-(ethoxycarbonyl)piperidin-1-yl]phenyl]methyl)piperazine-1-carboxylate




A flask was charged with ethyl 1-(5-chloro-2-formylphenyl)piperidine-4-carboxylate (1.33 g, 4.50 mmol, 1.00 equiv), tert-butyl piperazine-1-carboxylate (0.920 g, 4.94 mmol, 1.10 equiv), and 1,2-dichloroethane (10 mL). The resulting solution was stirred for 1 h at rt. NaBH(OAc)3 (2.86 g, 13.6 mmol, 3.00 equiv) was added at rt. The resulting solution was stirred for 3 h at rt and diluted with H2O (20 mL). The mixture was extracted with DCM (3×50 mL) and the organic layers were combined, washed with brine (3×50 mL), dried over anhydrous Na2SO4, filtered and concentrated. The residue was chromatographed on a silica gel column (3:7 EtOAc/petroleum ether) to provide tert-butyl 4-([4-chloro-2-[4-(ethoxycarbonyl)piperidin-1-yl]phenyl]methyl)piperazine-1-carboxylate (1.82 g, 87% yield) as colorless oil. LCMS (ESI, m/z): 466 [M+H]+.
Step 3: Preparation of 1-[2-([4-[(tert-butoxy)carbonyl]piperazin-1-yl]methyl)-5-chlorophenyl]piperidine-4-carboxylic acid




A flask was charged with tert-butyl 4-([4-chloro-2-[4-(ethoxycarbonyl)piperidin-1-yl]phenyl]methyl)piperazine-1-carboxylate (1.82 g, 3.91 mmol, 1.00 equiv), MeOH (10 mL), H2O (5 mL), and LiOH (0.469 g, 19.6 mmol, 5.00 equiv). The resulting solution was stirred for 3 h at rt. The pH value of the solution was adjusted to 7 with HCl (1 M, 1.5 mL). The mixture was extracted with DCM (3×50 mL) and the organic layers were combined, washed with brine (3×50 mL), dried over anhydrous Na2SO4, filtered and concentrated to provide 1-[2-([4-[(tert-butoxy)carbonyl]piperazin-1-yl]methyl)-5-chlorophenyl]piperidine-4-carboxylic acid (1.54 g, 90% yield) as a light yellow solid. LCMS (ESI, m/z): 438 [M+H]+.
Step 4: Preparation of 1-[5-chloro-2-(piperazin-1-ylmethyl)phenyl]piperidine-4-carboxylic acid




The title compound was synthesized according to the representative procedure of 60, Step 5, to provide 1-[5-chloro-2-(piperazin-1-ylmethyl)phenyl]piperidine-4-carboxylic acid as a light yellow solid. LCMS (ESI, m/z): 338 [M+H]+.
Step 5: Preparation of 1-(5-chloro-2-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)phenyl)piperidine-4-carboxylic acid




A flask was charged with triphosgene (59.4 mg, 0.200 mmol, 0.40 equiv) and DCM (5 mL). 1,1,1,3,3,3-Hexafluoropropan-2-ol (169 mg, 1.01 mmol, 1.00 equiv) was added dropwise at 0° C. DIEA (194 mg, 1.50 mmol, 3.00 equiv) was added dropwise at 0° C. The resulting solution was stirred for 2 h at rt. 1-[5-Chloro-2-(piperazin-1-ylmethyl)phenyl]piperidine-4-carboxylic acid (100 mg, 0.30 mmol, 1.20 equiv) was added. The resulting solution was stirred for 3 h at rt and diluted with H2O (20 mL). The mixture was extracted with DCM (3×30 mL) and the organic layers were combined, washed with brine (3×30 mL), dried over anhydrous Na2SO4, filtered and concentrated. The crude product (230 mg) was purified by preparative HPLC using the following gradient conditions: 20% CH3CN/80% NH4HCO3 (5%) increasing to 80% CH3CN over 10 min, then to 100% CH3CN over 0.1 min, holding at 100% CH3CN for 1.9 min, then reducing to 20% CH3CN over 0.1 min, and holding at 20% for 1.9 min, on a Waters 2767-5 Chromatograph. Column: Xbridge Prep C18, 19*150 mm 5 um; Detector, UV220 & 254 nm. Purification resulted in 1-(5-chloro-2-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)phenyl)piperidine-4-carboxylic acid (36.0 mg, 7% yield) as a light yellow solid. 1H NMR (400 MHz, Chloroform-d) δ 7.28-7.36 (m, 1H), 6.99-7.05 (m, 2H), 5.70-5.79 (m, 1H), 3.53 (br, 6H), 3.18 (d, J=11.6 Hz, 2H), 2.69 (t, J=10.8 Hz, 2H), 2.40 (br, 5H), 2.01-2.06 (m, 2H), 1.85-1.95 (m, 2H). LCMS (ESI, m/z): 532 [M+H]+.
Example 63
1-(2-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)piperidine-4-carboxylic acid




The title compound was synthesized directly from commercially available 2-fluoro-4-(trifluoromethyl)benzaldehyde according to the representative procedure of 62 to provide 1-(2-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)piperidine-4-carboxylic acid as a white solid. 1H NMR (300 MHz, Chloroform-d) δ 7.56-7.66 (m, 1H), 7.29-7.44 (m, 2H), 5.68-5.81 (m, 1H), 3.40-3.87 (m, 6H), 3.16-3.30 (m, 2H), 2.72-2.80 (m, 2H), 2.40-2.64 (m, 5H), 2.06-2.19 (m, 2H), 1.87-2.06 (m, 2H). LCMS (ESI, m/z): 566 [M+H]+.
Example 64
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(4-chloro-2-(5-cyclopropylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)benzyl)piperazine-1-carboxylate




Step 1: Preparation of tert-butyl 5-(5-chloro-2-formylphenyl)-octahydropyrrolo[3,4-c]pyrrole-2-carboxylate




A flask was charged with 4-chloro-2-fluorobenzaldehyde (0.791 g, 4.98 mmol, 1.00 equiv), tert-butyl octahydropyrrolo[3,4-c]pyrrole-2-carboxylate (1.27 g, 5.98 mmol, 1.50 equiv), DMSO (10 mL) and K2CO3 (2.00 g, 14.5 mmol, 2.00 equiv). The mixture was stirred overnight at 80° C. and diluted with H2O (20 mL). The resulting solution was extracted with EtOAc (3×20 mL) and the organic layers were combined, washed with H2O (3×10 mL), dried over anhydrous Na2SO4, filtered and concentrated. The residue was chromatographed on a silica gel column (1:1 EtOAc/petroleum ether) to provide tert-butyl 5-(5-chloro-2-formylphenyl)-octahydropyrrolo[3,4-c]pyrrole-2-carboxylate (1.40 g, 80% yield) as a yellow solid. LCMS (ESI, m/z): 351 [M+H]+.
Step 2: Preparation of tert-butyl 5-(5-chloro-2-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)phenyl)hexahydropyrrolo[3,4-c]pyrrole-2(1H)-carboxylate




A flask was charged with tert-butyl 5-(5-chloro-2-formylphenyl)-octahydropyrrolo[3,4-c]pyrrole-2-carboxylate (700 mg, 2.00 mmol, 1.00 equiv), 1,1,1,3,3,3-hexafluoropropan-2-yl piperazine-1-carboxylate (840 mg, 3.00 mmol, 1.50 equiv) [as prepared in 33, Step 2], 1,2-dichloroethane (20 mL) and NaBH(OAc)3 (848 mg, 4.00 mmol, 2.00 equiv). The resulting solution was stirred overnight at rt and quenched by H2O (20 mL). The mixture was extracted with DCM (3×20 mL) and the organic layers were combined, washed with H2O (3×10 mL), dried over anhydrous Na2SO4, filtered and concentrated. The residue was chromatographed on a silica gel column (1:1 EtOAc/petroleum ether) to provide tert-butyl 5-(5-chloro-2-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)phenyl)hexahydropyrrolo[3,4-c]pyrrole-2(1H)-carboxylate (800 mg, 65% yield) as a yellow oil. LCMS (ESI, m/z): 615 [M+H]+.
Step 3: Preparation of 1,1,1,3,3,3-hexafluoropropan-2-yl 4-[(4-chloro-2-[octahydropyrrolo[3,4-c]pyrrol-2-yl]phenyl)methyl]piperazine-1-carboxylate




A flask was charged with 1,1,1,3,3,3-hexafluoropropan-2-yl 4-[(2-[5-[(tert-butoxy)carbonyl]-octahydropyrrolo[3,4-c]pyrrol-2-yl]-4-chlorophenyl)methyl]piperazine-1-carboxylate (800 mg, 1.30 mmol, 1.00 equiv), DCM (10 mL) and TFA (5 mL). The resulting solution was stirred for 1 h at rt and concentrated to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-[(4-chloro-2-[octahydropyrrolo[3,4-c]pyrrol-2-yl]phenyl)methyl]piperazine-1-carboxylate (800 mg) as a yellow oil, which was carried on without further purification. LCMS (ESI, m/z): 515 [M+H]+.
Step 4: Preparation of 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(4-chloro-2-(5-cyclopropylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)benzyl)piperazine-1-carboxylate




A flask was charged with 1,1,1,3,3,3-hexafluoropropan-2-yl 4-[(4-chloro-2-[octahydropyrrolo[3,4-c]pyrrol-2-yl]phenyl)methyl]piperazine-1-carboxylate (205 mg, 0.401 mmol, 1.00 equiv), (1-ethoxycyclopropoxy)trimethylsilane (208 mg, 1.19 mmol, 3.00 equiv), THF (15 mL), acetic acid (98.0 mg, 1.63 mmol, 4.00 equiv) and sodium cyanoborohydride (80.0 mg, 1.27 mmol, 3.00 equiv). The resulting solution was stirred overnight at 60° C. and diluted with DCM (50 mL). The resulting mixture was washed with H2O (3×50 mL), dried over anhydrous Na2SO4, filtered and concentrated. The residue was purified by preparative HPLC using the following gradient conditions: 20% CH3CN/80% Phase A increasing to 80% CH3CN over 10 min, then to 100% CH3CN over 0.1 min, holding at 100% CH3CN for 1.9 min, then reducing to 20% CH3CN over 0.1 min, and holding at 20% for 1.9 min, on a Waters 2767-5 Chromatograph. Column: Xbridge Prep C18, 19*150 mm 5 um; Mobile phase: Phase A: aqueous NH4HCO3 (0.05%); Phase B: CH3CN; Detector, UV220 & 254 nm. Purification resulted in 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(4-chloro-2-(5-cyclopropylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)benzyl)piperazine-1-carboxylate (119.3 mg, 54% yield) as yellow oil. 1H NMR (400 MHz, Chloroform-d) δ 7.37 (d, J=6.1 Hz, 1H), 6.99-7.05 (m, 2H), 5.69-5.79 (m, 1H), 3.50-3.65 (m, 6H), 3.05-3.15 (m, 2H), 2.94-3.03 (m, 4H), 2.85 (s, 2H), 2.38-2.42 (m, 6H), 1.58-1.65 (m, 1H), 0.41-0.50 (m, 4H). LCMS (ESI, m/z): 555 [M+H]+.
Example 65
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(5-cyclopropylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly form commercially available 2-fluoro-4-(trifluoromethyl)benzaldehyde according to the representative procedure of 64 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(5-cyclopropylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate as a yellow oil. 1H NMR (400 MHz, chloroform-d) δ 7.60 (d, J=7.9 Hz, 1H), 7.25-7.29 (m, 2H), 5.72-5.81 (m, 1H), 3.58-3.61 (m, 6H), 3.12-3.16 (m, 2H), 2.99-3.12 (m, 4H), 2.88 (s, 2H), 2.42-3.60 (m, 6H), 1.66 (s, 1H), 0.42-0.58 (m, 4H). LCMS (ESI, m/z): 589 [M+H]+.
Example 66
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(4-chloro-2-(1-cyclopropyl-1,8-diazaspiro[4.5]decan-8-yl)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available tert-butyl 1,8-diazaspiro[4.5]decane-1-carboxylate according to the representative procedure of 64 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(4-chloro-2-(1-cyclopropyl-1,8-diazaspiro[4.5]decan-8-yl)benzyl)piperazine-1-carboxylate as a yellow oil. 1H NMR (400 MHz, chloroform-d) δ 7.38 (d, J=8.1 Hz, 1H), 7.02-7.08 (m, 2H), 5.69-5.81 (m, 1H), 3.49-3.61 (s, 6H), 3.08-3.18 (m, 2H), 2.93 (s, 2H), 2.72 (t, J=10.7 Hz, 2H), 2.44-2.53 (m, 4H), 2.16 (t, J=11.0 Hz, 2H), 1.70-1.91 (m, 5H), 1.45 (s, 2H), 0.35-0.62 (m, 4H). LCMS (ESI, m/z): 583 [M+H]+.
Example 67
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(1-cyclopropyl-1,8-diazaspiro[4.5]decan-8-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 2-fluoro-4-(trifluoromethyl)benzaldehyde and tert-butyl 1,8-diazaspiro[4.5]decane-1-carboxylate according to the representative procedure of 64 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(1-cyclopropyl-1,8-diazaspiro[4.5]decan-8-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate as a white solid. 1H NMR (300 MHz, chloroform-d) δ 7.55-7.63 (m, 1H), 7.31 (s, 2H), 5.70-5.81 (m, 1H), 3.50-3.65 (m, 6H), 3.02-3.20 (m, 2H), 2.91 (s, 2H), 2.71-2.82 (m, 2H), 3.50 (s, 4H), 2.18 (s, 2H), 1.70-1.95 (m, 4H), 1.56 (s, 2H), 1.36-1.51 (m, 2H), 0.35-0.62 (m, 3H). LCMS (ESI, m/z): 617 [M+H]+.
Example 68
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(4-(pyrrolidine-1-carbonyl)piperidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 2-fluoro-4-(trifluoromethyl)benzaldehyde and piperidin-4-yl(pyrrolidin-1-yl)methanone compound with methane (1:2) according to the representative procedure of 33, Steps 1-3 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(4-(pyrrolidine-1-carbonyl)piperidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate as a pale oil: 1H NMR (400 MHz, Chloroform-d) δ 7.59 (d, J=7.8 Hz, 1H), 7.35-7.30 (m, 2H), 5.84-5.70 (m, 1H), 3.64 (s, 2H), 3.61-3.48 (m, 8H), 3.32-3.24 (m, 2H), 2.81-2.69 (m, 2H), 2.57-2.45 (m, 5H), 2.13-1.96 (m, 4H), 1.95-1.80 (m, 4H). LCMS (ESI, m/z): 619.2 [M+H]+.
Example 69
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(4-(pyrrolidine-1-carbonyl)piperidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate




Step 1: Preparation of (2-formyl-5-(trifluoromethyl)phenyl)proline




A 50-mL round-bottom flask was charged with 2-fluoro-4-(trifluoromethyl)benzaldehyde (0.600 g, 3.12 mmol, 1.00 equiv), ethyl pyrrolidine-2-carboxylate (0.894 g, 6.24 mmol, 2.00 equiv), dimethyl sulfoxide (15 mL), and potassium carbonate (1.29 g, 9.33 mmol, 3.00 equiv) under nitrogen. The resulting solution was stirred overnight at 90° C. and quenched with water (20 mL). The resulting mixture was extracted with dichloromethane (3×30 mL) and the organic layers were combined, washed with brine (1×100 mL), dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was chromatographed on a silica gel column with ethyl acetate/petroleum ether (1/4) to provide 0.0800 g (9% yield) of 1-[2-formyl-5-(trifluoromethyl)phenyl]pyrrolidine-2-carboxylic acid as a yellow solid. LCMS (ESI, m/z): 288 [M+H]+.
Step 2: Preparation of 1-(tert-butyl) 4-(1,1,1,3,3,3-hexafluoropropan-2-yl) piperazine-1,4-dicarboxylate




A 500-mL round-bottom flask was charged with triphosgene (7.82 g, 26.3 mmol, 0.70 equiv), and dichloromethane (200 mL). 1,1,1,3,3,3-hexafluoropropan-2-ol (12.7 g, 75.6 mmol, 2.00 equiv) was added at 0° C., followed by N,N-Diisopropylethylamine (19.4 g, 150 mmol, 4.00 equiv). The mixture was stirred for 2 h at room temperature after which tert-butyl piperazine-1-carboxylate (7.00 g, 37.6 mmol, 1.00 equiv) was added. The resulting solution was stirred overnight at room temperature and quenched with water (150 mL). The resulting mixture was extracted with dichloromethane (3×250 mL) and the organic layers were combined, washed with brine (1×300 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The residue was chromatographed on a silica gel column with ethyl acetate/petroleum ether (1/9) to provide 4.70 g (33% yield) of 1-tert-butyl 4-(1,1,1,3,3,3-hexafluoropropan-2-yl) piperazine-1,4-dicarboxylate as a white solid. LCMS (ESI, m/z): 381 [M+H]+.
Step 3: Preparation of 1,1,1,3,3,3-hexafluoropropan-2-yl piperazine-1-carboxylate




A 50-mL round-bottom flask was charged with 1-tert-butyl 4-(1,1,1,3,3,3-hexafluoropropan-2-yl) piperazine-1,4-dicarboxylate (750 mg, 1.97 mmol, 1.00 equiv), dichloromethane (10 mL), and trifluoroacetic acid (4 mL). The resulting solution was stirred overnight at room temperature and concentrated under reduced pressure to provide 900 mg (crude) of 1,1,1,3,3,3-hexafluoropropan-2-yl piperazine-1-carboxylate as a yellow solid. LCMS (ESI, m/z): 281 [M+H]+.
Step 4: Preparation of 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(4-(pyrrolidine-1-carbonyl)piperidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate




A 50-mL round-bottom flask was charged with 1,1,1,3,3,3-hexafluoropropan-2-yl piperazine-1-carboxylate (85.6 mg, 0.310 mmol, 1.10 equiv), 1-[2-formyl-5-(trifluoromethyl)phenyl]pyrrolidine-2-carboxylic acid (80.0 mg, 0.280 mmol, 1.00 equiv), and 1,2-dichloroethane (10 mL). The mixture was stirred for 1 h at room temperature. Sodium triacetoxyborohydride (177 mg, 0.830 mmol, 3.00 equiv) was added. The resulting solution was stirred overnight at room temperature and quenched with water (10 mL). The resulting mixture was extracted with dichloromethane (3×15 mL) and the organic layers were combined, washed with brine (1×50 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The crude product (400 mg) was purified by preparative HPLC using the following gradient conditions: 20% CH3CN/80% Phase A increasing to 80% CH3CN over 10 min, then to 100% CH3CN over 0.1 min, holding at 100% CH3CN for 1.9 min, then reducing to 20% CH3CN over 0.1 min, and holding at 20% for 1.9 min, on a Waters 2767-5 Chromatograph. Column: Xbridge Prep C18, 19*150 mm 5 um; Mobile phase: Phase A: aqueous NH4HCO3 (0.05%); Phase B: CH3CN; Detector, UV220 & 254 nm. Purification resulted in 45.6 mg (30% yield) of 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(4-(pyrrolidine-1-carbonyl)piperidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate as a white solid. 1H NMR (300 MHz, Chloroform-d) δ 13.5 (br, 1H), 7.50 (s, 1H), 7.40 (d, J=7.8 Hz, 1H), 7.29 (d, J=7.8 Hz, 1H), 5.69-5.77 (m, 1H), 4.36-4.41 (m, 1H), 4.17-4.21 (m, 1H), 3.56-3.65 (m, 5H), 3.07-3.11 (m, 1H), 2.84-2.92 (m, 1H), 2.50-2.62 (m, 5H), 2.21-2.30 (m, 1H), 1.96-2.10 (m, 2H). LCMS (ESI, m/z): 552 [M+H]+.
Example 70
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(2-(ethoxycarbonyl)pyrrolidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate




Step 1: Preparation of ethyl (2-formyl-5-(trifluoromethyl)phenyl)prolinate




A 50-mL round-bottom flask was charged with 2-bromo-4-(trifluoromethyl)benzaldehyde (0.300 g, 1.19 mmol, 1.00 equiv), ethyl pyrrolidine-2-carboxylate (0.340 g, 2.37 mmol, 2.00 equiv), cesium carbonate (1.16 g, 3.57 mmol, 3.00 equiv), dicyclohexyl(2′,6′-diisopropoxybiphenyl-2-yl)phosphine (0.222 g, 0.480 mmol, 0.40 equiv), tris(dibenzylideneacetone)dipalladium (0.109 g, 0.120 mmol, 0.10 equiv), and toluene (10 mL) under nitrogen. The resulting solution was stirred overnight at 100° C. and quenched with water (15 mL). The resulting mixture was extracted with dichloromethane (3×25 mL). The organic layers were combined and washed with brine (1×60 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The residue was chromatographed on a silica gel column with ethyl acetate/petroleum ether (1/5) to provide 0.0700 g (19% yield) of ethyl 1-[2-formyl-5-(trifluoromethyl)phenyl]pyrrolidine-2-carboxylate as a yellow solid. LCMS (ESI, m/z): 316 [M+H]+.
Step 2: Preparation of 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(2-(ethoxycarbonyl)pyrrolidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate
The title compound was prepared according to the representative procedure of Example 69, Steps 2-4, using ethyl (2-formyl-5-(trifluoromethyl)phenyl)prolinate in Step 4 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-([2-[2-(ethoxycarbonyl)pyrrolidin-1-yl]-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate as yellow oil. 1H NMR (300 MHz, Chloroform-d) δ 7.58-7.61 (m, 1H), 7.20-7.28 (m, 2H), 5.70-5.78 (m, 1H), 4.41-4.43 (m, 1H), 3.98-4.05 (m, 2H), 3.64-3.69 (m, 7H), 3.12 (br, 1H), 2.36-2.56 (m, 5H), 1.95-2.13 (m, 3H), 1.08-1.12 (m, 3H). LCMS (ESI, m/z): 580 [M+H]+.
Example 71
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(2-(hydroxymethyl)pyrrolidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 2-fluoro-4-(trifluoromethyl)benzaldehyde and pyrrolidin-2-ylmethanol according to the representative procedure of 69, Steps 1-4 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(2-(hydroxymethyl)pyrrolidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate as yellow oil. 1H NMR (300 MHz, Chloroform-d) δ 7.50 (s, 1H), 7.25-7.31 (m, 2H), 5.68-5.77 (m, 1H), 5.14 (br, 1H), 4.16-4.20 (m, 1H), 3.72-3.74 (m, 1H), 3.58-3.59 (m, 4H), 3.32-3.50 (m, 3H), 2.94-2.98 (m, 1H), 2.80-2.87 (m, 1H), 2.47-2.49 (m, 4H), 1.95-2.12 (m, 4H). LCMS (ESI, m/z): 538 [M+H]+.
Example 72
1-(2-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)pyrrolidine-3-carboxylic acid




Step 1: Preparation of methyl 1-(2-formyl-5-(trifluoromethyl)phenyl)pyrrolidine-3-carboxylate




A 50-mL round-bottom flask was charged with 2-fluoro-4-(trifluoromethyl)benzaldehyde (0.600 g, 3.12 mmol, 1.00 equiv), methyl pyrrolidine-3-carboxylate (0.806 g, 6.24 mmol, 2.00 equiv), dimethyl sulfoxide (15 mL), and N,N-Diisopropylethylamine (1.21 g, 9.36 mmol, 3.00 equiv) under nitrogen. The resulting solution was stirred overnight at 70° C. and quenched with water (20 mL). The resulting mixture was extracted with dichloromethane (3×30 mL) and the organic layers were combined, washed with brine (1×100 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The residue was chromatographed on a silica gel column with ethyl acetate/petroleum ether (1/9) to provide 0.165 g (18% yield) of methyl 1-(2-formyl-5-(trifluoromethyl)phenyl)pyrrolidine-3-carboxylate as a yellow solid. LCMS (ESI, m/z): 302 [M+H]+.
Step 2: Preparation of 1-(2-formyl-5-(trifluoromethyl)phenyl)pyrrolidine-3-carboxylic acid




A 50-mL round-bottom flask was charged with methyl 1-[2-formyl-5-(trifluoromethyl)phenyl]pyrrolidine-3-carboxylate (165 mg, 0.550 mmol, 1.00 equiv), tetrahydrofuran (9 mL), lithium hydroxide (39.5 mg, 1.65 mmol, 3.00 equiv), and water (3 mL). The resulting solution was stirred overnight at room temperature and quenched with water (10 mL). The PH of the solution was adjusted to 5 with hydrochloric acid (1M, 3 mL). The resulting solution was extracted with ethyl acetate (3×15 mL) and the organic layers were combined, washed with brine (1×50 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to provide 150 mg (95% yield) of 1-(2-formyl-5-(trifluoromethyl)phenyl)pyrrolidine-3-carboxylic acid as a white solid. LCMS (ESI, m/z): 288 [M+H]+.
Step 3: Preparation of 1-(2-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)pyrrolidine-3-carboxylic acid
The title compound was prepared according to the representative procedure of Example 69, Steps 2-4, using 1-(2-formyl-5-(trifluoromethyl)phenyl)pyrrolidine-3-carboxylic acid in Step 4 to provide 1-(2-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)pyrrolidine-3-carboxylic acid as a white solid. 1H NMR (300 MHz, Chloroform-d) δ 7.50 (d, J=7.8 Hz, 1H), 7.20-7.24 (m, 2H), 5.68-5.81 (m, 1H), 3.52-3.69 (m, 7H), 3.32-3.39 (m, 2H), 3.16-3.26 (m, 2H), 2.46-2.47 (m, 4H), 2.28-2.39 (m, 2H). LCMS (ESI, m/z): 552 [M+H]+.
Example 73
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(3-(hydroxymethyl)pyrrolidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 2-fluoro-4-(trifluoromethyl)benzaldehyde and pyrrolidin-3-ylmethanol according to the representative procedure of 69, Steps 1-4 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(3-(hydroxymethyl)pyrrolidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate as yellow oil. 1H NMR (300 MHz, Chloroform-d) δ 7.48-7.50 (m, 1H), 7.16-7.18 (m, 2H), 5.71-5.79 (m, 1H), 3.75-3.80 (m, 1H), 3.59-3.70 (m, 7H), 3.11-3.40 (m, 4H), 2.47-2.57 (m, 5H), 2.09-2.20 (m, 2H), 1.80-1.89 (m, 1H). LCMS (ESI, m/z): 538 [M+H]+.
Example 74
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(4-(hydroxymethyl)piperidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate




The title compound was synthesized directly from commercially available 2-fluoro-4-(trifluoromethyl)benzaldehyde and piperidin-4-ylmethanol according to the representative procedure of 69, Steps 1-4 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(4-(hydroxymethyl)piperidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate as colorless oil. 1H NMR (300 MHz, Chloroform-d) δ 7.57-7.60 (m, 1H), 7.30 (br, 2H), 5.69-5.81 (m, 1H), 3.56-3.60 (m, 8H), 3.10-3.18 (m, 2H), 2.61-2.74 (m, 2H), 2.48-2.50 (m, 4H), 1.83-1.87 (m, 2H), 1.60-1.71 (m, 1H), 1.42-1.50 (m, 3H). LCMS (ESI, m/z): 552 [M+H]+.
Example 75
4-(2-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)morpholine-2-carboxylic acid




The title compound was synthesized directly from commercially available 2-fluoro-4-(trifluoromethyl)benzaldehyde and methyl morpholine-2-carboxylate according to the representative procedure of 70, Steps 1-2 to provide 4-(2-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)morpholine-2-carboxylic acid as a white solid. 1H NMR (300 MHz, Chloroform-d) δ 7.51-7.54 (m, 1H), 7.37-7.39 (m, 1H), 7.33 (s, 1H), 5.67-5.78 (m, 1H), 4.34-3.38 (m, 1H), 4.16-4.20 (m, 1H), 3.82-3.93 (m, 2H), 3.56-3.67 (m, 6H), 3.00-3.03 (m, 2H), 2.90-2.94 (m, 1H), 2.56 (br, 4H). LCMS (ESI, m/z): 568 [M+H]+.
Example 76
2-(2-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)-2-oxoacetic acid




Step 1: Preparation of tert-butyl 4-(2-bromo-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate




A 100-mL round-bottom flask was charged with 2-bromo-4-(trifluoromethyl)benzaldehyde (1.10 g, 4.35 mmol, 1.00 equiv), tert-butyl piperazine-1-carboxylate (1.06 g, 5.69 mmol, 1.30 equiv), and 1.2-dichloroehane (10 mL). The mixture was stirred for 1 h at room temperature. Sodium triacetoxyborohydride (2.78 g, 13.1 mmol, 3.00 equiv) was added. The resulting solution was stirred overnight at room temperature and quenched with water (15 mL). The mixture was extracted with dichloromethane (3×20 mL) and the organic layers were combined, washed with brine (1×60 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The residue was chromatographed on a silica gel column with ethyl acetate/petroleum ether (1/4) to provide 1.40 g (76% yield) of tert-butyl 4-(2-bromo-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate as colorless oil. LCMS (ESI, m/z): 423 [M+H]+.
Step 2: Preparation of tert-butyl 4-(2-(2-ethoxy-2-oxoacetyl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate




A 50-mL round-bottom flask was charged with tert-butyl 4-[[2-bromo-4-(trifluoromethyl)benzyl]piperazine-1-carboxylate (0.360 g, 0.850 mmol, 1.00 equiv) and tetrahydrofuran (8 mL) under nitrogen. The mixture was cooled to −78° C. n-Butyllithium (2.5 M in hexane, 0.52 mL, 1.28 mmol, 1.50 equiv) was added at −78° C. The mixture was stirred for 1 h at −78° C. and diethyl oxalate (1.99 g, 13.6 mmol, 16.00 equiv) was added. The resulting solution was stirred for 2 h at −78° C. and quenched with saturated ammonium chloride aqueous solution (10 mL). The resulting solution was extracted with dichloromethane (3×15 mL) and the organic layers were combined, washed with brine (1×50 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The residue was chromatographed on a silica gel column with ethyl acetate/petroleum ether (1/5) to provide 0.0850 g (22% yield) of 4-(2-(2-ethoxy-2-oxoacetyl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate as a light yellow solid. LCMS (ESI, m/z): 445 [M+H]+.
Step 3: Preparation of 2-(2-((4-(tert-butoxycarbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)-2-oxoacetic acid




A 50-mL round-bottom flask was charged with tert-butyl 4-[[2-(2-ethoxy-2-oxoacetyl)-4-(trifluoromethyl)phenyl]methyl]piperazine-1-carboxylate (70.0 mg, 0.160 mmol, 1.00 equiv), tetrahydrofuran (4 mL), lithium hydroxide (37.8 mg, 1.58 mmol, 10.00 equiv), and water (1 mL). The resulting solution was stirred overnight at room temperature and quenched with water (5 mL). The pH of the solution was adjusted to 5 with hydrochloric acid (1M). The resulting solution was extracted with ethyl acetate (3×10 mL) and the organic layers were combined, washed with brine (1×20 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to provide 65.0 mg (99% yield) of 2-(2-((4-(tert-butoxycarbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)-2-oxoacetic acid as colorless oil. LCMS (ESI, m/z): 417 [M+H]+.
Step 4: Preparation of 2-oxo-2-(2-(piperazin-1-ylmethyl)-5-(trifluoromethyl)phenyl)acetic acid




A 50-mL round-bottom flask was charged with 2-[2-([4-[(tert-butoxy)carbonyl]piperazin-1-yl]methyl)-5-(trifluoromethyl)phenyl]-2-oxoacetic acid (65.0 mg, 0.160 mmol, 1.00 equiv), dichloromethane (8 mL), and trifluoroacetic acid (2 mL). The resulting solution was stirred overnight at room temperature and concentrated under reduced pressure to provide 100 mg (crude) of 2-oxo-2-(2-(piperazin-1-ylmethyl)-5-(trifluoromethyl)phenyl)acetic acid as colorless oil. LCMS (ESI, m/z): 317 [M+H]+.
Step 5: Preparation of 2-(2-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)-2-oxoacetic acid




A 50-mL round-bottom flask was charged with triphosgene (31.6 mg, 0.110 mmol, 0.70 equiv), and dichloromethane (8 mL). 1,1,1,3,3,3-hexafluoropropan-2-ol (51.0 mg, 0.300 mmol, 2.00 equiv) was added at 0° C. N,N-Diisopropylethylamine (78.4 mg, 0.610 mmol, 4.00 equiv) was added at 0° C. The mixture was stirred for 2 h at room temperature. 2-oxo-2-(2-(piperazin-1-ylmethyl)-5-(trifluoromethyl)phenyl)acetic acid (48.0 mg, 0.150 mmol, 1.00 equiv) was added. The resulting solution was stirred overnight at room temperature and quenched with water (10 mL). The resulting mixture was extracted with dichloromethane (3×15 mL) and the organic layers were combined, washed with brine (1×50 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The crude product (200 mg) was purified by preparative HPLC using the following gradient conditions: 20% CH3CN/80% Phase A increasing to 80% CH3CN over 10 min, then to 100% CH3CN over 0.1 min, holding at 100% CH3CN for 1.9 min, then reducing to 20% CH3CN over 0.1 min, and holding at 20% for 1.9 min, on a Waters 2767-5 Chromatograph. Column: Xbridge Prep C18, 19*150 mm 5 um; Mobile phase: Phase A: aqueous NH4HCO3 (0.05%); Phase B: CH3CN; Detector, UV220 & 254 nm. Purification resulted in 21.1 mg (27% yield) of 2-(2-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)-2-oxoacetic acid as a white solid. 1H NMR (300 MHz, Chloroform-d) δ 8.18 (br, 2H), 7.66 (br, 1H), 5.62-5.75 (m, 1H), 3.47 (br, 6H), 2.36 (br, 4H). LCMS (ESI, m/z): 511 [M+H]+.
Example 77
(5-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-2-(trifluoromethyl)phenyl)proline




Step 1: Preparation of tert-butyl 4-(3-(2-(ethoxycarbonyl)pyrrolidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate




The listed intermediate was prepared according to the representative procedure of 52, Steps 1-2, using ethyl prolinate in Step 2 to provide tert-butyl 4-(3-(2-(ethoxycarbonyl)pyrrolidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate as yellow oil. LCMS (ESI, m/z): 486 [M+H]+.
Step 2: Preparation of (5-((4-(tert-butoxycarbonyl)piperazin-1-yl)methyl)-2-(trifluoromethyl)phenyl)proline




A 100-mL round-bottom flask was charged with tert-butyl tert-butyl 4-(3-(2-(ethoxycarbonyl)pyrrolidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate (200 mg, 0.410 mmol, 1.00 equiv), tetrahydrofuran (10 mL), water (2 mL), and lithium hydroxide (98.4 mg, 4.11 mmol, 10.0 equiv). The resulting solution was stirred overnight at room temperature and quenched with water (10 mL). The pH of the solution was adjusted to 5 with hydrochloric acid (1M, 3 mL). The resulting solution was extracted with ethyl acetate (3×15 mL) and the organic layers were combined, washed with brine (1×50 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to provide 180 mg (96% yield) of (5-((4-(tert-butoxycarbonyl)piperazin-1-yl)methyl)-2-(trifluoromethyl)phenyl)proline as yellow oil. LCMS (ESI, m/z): 458 [M+H]+.
Step 3: Preparation of (5-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-2-(trifluoromethyl)phenyl)proline
The title compound was prepared from (5-((4-(tert-butoxycarbonyl)piperazin-1-yl)methyl)-2-(trifluoromethyl)phenyl)proline according to the representative procedure of 52, Steps 3-4 to provide (5-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-2-(trifluoromethyl)phenyl)proline as a white solid. 1H NMR (300 MHz, Methanol-d4) δ 7.50 (s, 1H), δ 7.18 (s, 1H), 6.93-6.96 (m, 1H), 6.08-6.21 (m, 1H), 4.38 (t, J=6.3 Hz, 1H), 3.71-3.79 (m, 1H), 3.55-3.62 (m, 6H), 3.23-3.27 (m, 1H), 2.37-2.50 (m, 5H), 1.91-2.13 (m, 3H). LCMS (ESI, m/z): 552 [M+H]+.
Example 78
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-(2-(ethoxycarbonyl)pyrrolidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate




The title compound was prepared from 3-bromo-4-(trifluoromethyl)benzaldehyde according to the representative procedure of 52, Steps 1-4, using ethyl prolinate in Step 2 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-(2-(ethoxycarbonyl)pyrrolidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate as a light yellow oil. 1H NMR (300 MHz, Chloroform-d) δ 7.51 (s, 1H), δ 7.09 (s, 1H), 6.93 (d, J=6.0 Hz, 1H), 5.71-5.79 (m, 1H), 4.42-4.46 (m, 1H), 4.01-4.08 (m, 2H), 3.68-3.76 (m, 1H), 3.46-3.57 (m, 6H), 3.22-3.29 (m, 1H), 2.35-2.45 (m, 5H), 1.89-2.14 (m, 3H), 1.12 (t, J=6.9 Hz, 3H). LCMS (ESI, m/z): 580 [M+H]+.
Example 79
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-(2-(hydroxymethyl)pyrrolidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate




The title compound was prepared from 3-bromo-4-(trifluoromethyl)benzaldehyde according to the representative procedure of 52, Steps 1-4, using pyrrolidin-2-ylmethanol in Step 2 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-(2-(hydroxymethyl)pyrrolidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate as a yellow oil. 1H NMR (300 MHz, Chloroform-d) δ 7.57 (s, 1H), δ 7.32 (s, 1H), 7.12 (d, J=8.1 Hz, 1H), 5.69-5.81 (m, 1H), 3.77 (br, 1H), 3.50-3.58 (m, 8H), 3.40-3.50 (m, 1H), 2.87-2.95 (m, 1H), 2.46 (br, 4H), 1.81-2.24 (m, 5H). LCMS (ESI, m/z): 538 [M+H]+.
Example 80
1-(5-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-2-(trifluoromethyl)phenyl)pyrrolidine-3-carboxylic acid




The title compound was prepared according to the representative procedure of Example 77, using methyl pyrrolidine-3-carboxylate in Step 1, to provide 1-(5-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-2-(trifluoromethyl)phenyl)pyrrolidine-3-carboxylic acid as a white solid. 1H NMR (300 MHz, Methanol-d4) δ 7.59 (d, J=8.1 Hz, 1H), δ 7.23 (s, 1H), 7.02 (d, J=8.1 Hz, 1H), 6.12-6.25 (m, 1H), δ 3.84 (s, 2H), 3.78 (br, 4H), 3.58-3.68 (m, 2H), 3.31-3.42 (m, 2H), 3.14-3.24 (m, 1H), 2.79 (br, 4H), 2.20-2.28 (m, 2H). LCMS (ESI, m/z): 552 [M+H]+.
Example 81
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-(3-(hydroxymethyl)pyrrolidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate




The title compound was prepared according to the representative procedure of Example 52, Steps 1-4 using 3-bromo-4-(trifluoromethyl)benzaldehyde in Step 1 and pyrrolidin-3-ylmethanol in Step 2 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-(3-(hydroxymethyl)pyrrolidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate as a yellow oil. 1H NMR (300 MHz, Chloroform-d) δ 7.52 (s, 1H), δ 7.02 (s, 1H), 6.90 (d, J=8.1 Hz, 1H), 5.71-5.79 (m, 1H), 3.67-3.76 (m, 2H), 3.51-3.64 (m, 6H), 3.18-3.43 (m, 4H), 2.47-2.56 (m, 5H), 2.01-2.17 (m, 1H), 1.73-1.85 (m, 2H). LCMS (ESI, m/z): 538 [M+H]+.
Example 82
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-(4-(hydroxymethyl)piperidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate




The title compound was prepared according to the representative procedure of Example 52, Steps 1-4 using 3-bromo-4-(trifluoromethyl)benzaldehyde in Step 1 and piperidin-4-ylmethanol in Step 2 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-(4-(hydroxymethyl)piperidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate as a yellow oil. 1H NMR (300 MHz, Chloroform-d) δ 7.56 (d, J=8.1 Hz, 1H), 7.36 (s, 1H), 7.15 (d, J=7.8 Hz, 1H), 5.68-5.81 (m, 1H), 3.33-3.80 (m, 8H), 3.09-3.13 (m, 2H), 2.69-2.76 (m, 2H), 2.47 (br, 4H), 1.78-1.82 (m, 2H), 1.58-1.69 (m, 1H), 1.43-1.51 (m, 3H). LCMS (ESI, m/z): 552 [M+H]+.
Example 83
4-(5-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-2-(trifluoromethyl)phenyl)morpholine-2-carboxylic acid




The title compound was prepared according to the representative procedure of Example 77, using 3-bromo-4-(trifluoromethyl)benzaldehyde and methyl morpholine-2-carboxylate in Step 1 to provide 4-(5-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-2-(trifluoromethyl)phenyl)morpholine-2-carboxylic acid as a white solid. 1H NMR (300 MHz, Methanol-d4) δ 7.62 (d, J=8.1 Hz, 1H), 7.50 (s, 1H), 7.32 (d, J=8.1 Hz, 1H), 6.09-6.18 (m, 1H), 4.16-4.19 (m, 1H), 4.05-4.08 (m, 1H), 3.77-3.84 (m, 1H), 3.58-3.62 (m, 6H), 3.26-3.32 (m, 1H), 2.83-2.95 (m, 3H), 2.50 (br, 4H). LCMS (ESI, m/z): 568 [M+H]+.
Example 84
2-(5-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-2-(trifluoromethyl)phenyl)-2-oxoacetic acid




The title compound was prepared according to the representative procedure of Example 76, using 3-bromo-4-(trifluoromethyl) benzaldehyde in Step 1 to provide 2-(5-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-2-(trifluoromethyl)phenyl)-2-oxoacetic acid as a white solid. 1H NMR (300 MHz, Methanol-d4) δ 7.75-7.79 (m, 2H), 7.65-7.68 (m, 1H), 6.08-6.21 (m, 1H), 3.66 (s, 2H), 3.56-3.61 (m, 4H), 2.47-2.53 (m, 4H). LCMS (ESI, m/z): 511 [M+H]+.
Example 85
1-(5-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-2-(trifluoromethyl)phenyl)piperidine-4-carboxylic acid




The title compound was prepared according to the representative procedure of Example 77, using 3-bromo-4-(trifluoromethyl)benzaldehyde and ethyl piperidine-4-carboxylate in Step 1 to provide 1-(5-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-2-(trifluoromethyl)phenyl)piperidine-4-carboxylic acid as a white solid. 1H NMR (300 MHz, Methanol-d4) δ 7.58 (d, J=7.8 Hz, 1H), 7.46 (s, 1H), 7.26 (d, J=8.1 Hz, 1H), 6.12-6.16 (m, 1H), 3.60 (s, 6H), 3.03-3.07 (m, 2H), 2.77-2.84 (m, 2H), 2.38-2.49 (m, 5H), 1.83-1.99 (m, 4H). LCMS (ESI, m/z): 566 [M+H]+.
Example 86
(3-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)proline




Step 1: Preparation of 1-(tert-butyl) 4-(1,1,1,3,3,3-hexafluoropropan-2-yl) piperazine-1,4-dicarboxylate




A 250-mL round-bottom flask was charged with triphosgene (5.60 g, 18.9 mmol, 0.70 equiv), and dichloromethane (50 mL). 1,1,1,3,3,3-hexafluoropropan-2-ol (9.00 g, 53.6 mmol, 2.00 equiv) was added at 0° C. Pyridine (12.7 g, 161 mmol, 6.00 equiv) was added at 0° C. The mixture was stirred for 2 h at room temperature. tert-Butyl piperazine-1-carboxylate (5.00 g, 26.9 mmol, 1.00 equiv) was added. The resulting solution was stirred overnight at room temperature and then quenched with water (30 mL). The resulting mixture was extracted with dichloromethane (3×30 mL) and the organic layers were combined, washed with brine (1×100 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The residue was chromatographed on a silica gel column with ethyl acetate/petroleum ether (15/85) to provide 9.20 g (90% yield) of 1-tert-butyl 4-(1,1,1,3,3,3-hexafluoropropan-2-yl) piperazine-1,4-dicarboxylate as a yellow solid. LCMS (ESI, m/z): 381 [M+H]+.
Step 2: Preparation of 1,1,1,3,3,3-hexafluoropropan-2-yl piperazine-1-carboxylate




A 40-mL round-bottom flask was charged with 1-tert-butyl 4-(1,1,1,3,3,3-hexafluoropropan-2-yl) piperazine-1,4-dicarboxylate (433 mg, 1.14 mmol, 1.00 equiv), dichloromethane (5 mL), and trifluoroacetic acid (2 mL). The resulting solution was stirred overnight at room temperature and concentrated under reduced pressure to afford 500 mg (crude) of 1,1,1,3,3,3-hexafluoropropan-2-yl piperazine-1-carboxylate as colorless oil. LCMS (ESI, m/z): 281 [M+H]+.
Step 3: Preparation of ethyl (3-formyl-5-(trifluoromethyl)phenyl)prolinate




A 100-mL round-bottom flask was charged with 3-bromo-5-(trifluoromethyl)benzaldehyde (1.01 g, 3.99 mmol, 1.00 equiv), ethyl pyrrolidine-2-carboxylate (0.686 g, 4.79 mmol, 1.20 equiv), tris(dibenzylideneacetone)dipalladium (0.182 g, 0.199 mmol, 0.05 equiv), dicyclohexyl(2′,6′-diisopropoxybiphenyl-2-yl)phosphine (0.372 g, 0.798 mmol, 0.20 equiv), cesium carbonate (3.91 g, 12.0 mmol, 3.00 equiv), and toluene (10 mL) under nitrogen. The resulting solution was stirred overnight at 100° C. and quenched with water (20 mL). The mixture was extracted with ethyl acetate (3×30 mL) and the organic layers were combined, washed with brine (3×30 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The residue was chromatographed on a silica gel column with ethyl acetate/petroleum ether (1/9) to provide 0.480 g (38% yield) of ethyl 1-[3-formyl-5-(trifluoromethyl)phenyl]pyrrolidine-2-carboxylate as yellow oil. LCMS (ESI, m/z): 316 [M+H]+.
Step 4: Preparation of (3-formyl-5-(trifluoromethyl)phenyl)proline




A 40-mL round-bottom flask was charged with ethyl 1-[3-formyl-5-(trifluoromethyl)phenyl]pyrrolidine-2-carboxylate (300 mg, 0.952 mmol, 1.00 equiv), lithium hydroxide (114 mg, 4.76 mmol, 5.00 equiv), tetrahydrofuran (3 mL), and water (1 mL). The resulting solution was stirred overnight at room temperature. The pH of the solution was adjusted to 5 with hydrochloric acid (1M, 5 mL). The mixture was extracted with dichloromethane (3×30 mL) and the organic layers were combined, washed with brine (3×30 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to provide 250 mg (91% yield) of 1-[3-formyl-5-(trifluoromethyl)phenyl]pyrrolidine-2-carboxylic acid as yellow oil. LCMS (ESI, m/z): 288 [M+H]+.
Step 5: Preparation of (3-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)proline




A 40-mL round-bottom flask was charged with 1-[3-formyl-5-(trifluoromethyl)phenyl]pyrrolidine-2-carboxylic acid (155 mg, 0.540 mmol, 1.00 equiv), 1,1,1,3,3,3-hexafluoropropan-2-yl piperazine-1-carboxylate (179 mg, 0.648 mmol, 1.20 equiv), and 1,2-dichloroethane (5 mL), triethylamine (164 mg, 1.62 mmol, 3.00 equiv). The resulting solution was stirred for 2 h at room temperature. Sodium triacetoxyborohydride (343 mg, 1.62 mmol, 3.00 equiv) was added. The resulting solution was stirred overnight at room temperature and quenched with water (20 mL). The mixture was extracted with dichloromethane (3×30 mL) and the organic layers were combined, washed with brine (3×30 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The crude product (300 mg) was purified by preparative HPLC using the following gradient conditions: 20% CH3CN/80% Phase A increasing to 80% CH3CN over 10 min, then to 100% CH3CN over 0.1 min, holding at 100% CH3CN for 1.9 min, then reducing to 20% CH3CN over 0.1 min, and holding at 20% for 1.9 min, on a Waters 2767-5 Chromatograph. Column: Xbridge Prep C18, 19*150 mm 5 um; Mobile phase: Phase A: aqueous NH4HCO3 (0.05%); Phase B: CH3CN; Detector, UV220 & 254 nm. Purification resulted in 52.1 mg (18% yield) of (3-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)proline as a white solid. 1H NMR (300 MHz, Methanol-d4) δ 6.89 (s, 1H), 6.77 (s, 1H), 6.67 (s, 1H), 6.09-6.18 (m, 1H), 4.18-4.22 (m, 1H), 3.57 (br, 7H), 3.31-3.57 (m, 1H), 2.51-2.52 (m, 4H), 2.33-2.40 (m, 1H), 2.07-2.22 (m, 3H). LCMS (ESI, m/z): 552 [M+H]+.
Example 87
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-(2-(ethoxycarbonyl)pyrrolidin-1-yl)-5-(trifluoromethyl)benzyl)piperazine-1-carboxylate




The title compound was prepared according to the representative procedure of Example 86, Steps 1, 2, 4 and 5, using ethyl prolinate in Step 3 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-(2-(ethoxycarbonyl)pyrrolidin-1-yl)-5-(trifluoromethyl)benzyl)piperazine-1-carboxylate as a light yellow oil. 1H NMR (300 MHz, Chloroform-d) δ 6.91 (s, 1H), 6.66 (s, 2H), 5.72-5.78 (m, 1H), 4.10-4.29 (m, 3H), 3.52-3.63 (m, 7H), 3.37-3.45 (m, 1H), 2.48 (br, 4H), 2.07-2.35 (m, 4H), 1.26 (t, J=7.2 Hz, 3H). LCMS (ESI, m/z): 580 [M+H]+.
Example 88
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-(2-(hydroxymethyl)pyrrolidin-1-yl)-5-(trifluoromethyl)benzyl)piperazine-1-carboxylate




The title compound was prepared according to the representative procedure of Example 86, Steps 1, 2, 4 and 5, using pyrrolidin-2-ylmethanol in Step 3 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-(2-(hydroxymethyl)pyrrolidin-1-yl)-5-(trifluoromethyl)benzyl)piperazine-1-carboxylate as a light yellow oil. 1H NMR (400 MHz, Chloroform-d) δ 6.88 (s, 1H), 6.83 (br, 1H), 6.77 (s, 1H), 5.70-5.79 (m, 1H), 3.91 (br, 1H), 3.50-3.91 (m, 9H), 3.15-3.21 (m, 1H), 2.61 (br, 4H), 1.98-2.17 (m, 4H). LCMS (ESI, m/z): 538 [M+H]+.
Example 89
1-(3-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)pyrrolidine-3-carboxylic acid




The title compound was prepared according to the representative procedure of Example 77, Steps 1-5, using 3-bromo-5-(trifluoromethyl)benzaldehyde in Step 1, and using methyl pyrrolidine-3-carboxylate in Step 2 as well as replacing the palladium coupling conditions of Step 2 with those used in Step 3 of 86 to provide 1-(3-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)pyrrolidine-3-carboxylic acid as a light yellow solid. 1H NMR (400 MHz, Chloroform-d) δ 6.81 (s, 1H), 6.77 (s, 1H), 6.70 (s, 1H), 5.70-5.79 (m, 1H), 3.46-3.62 (m, 9H), 3.36-3.42 (m, 1H), 3.21-3.29 (m, 1H), 2.59 (br, 4H), 2.31-2.38 (m, 2H). LCMS (ESI, m/z): 552 [M+H]+.
Example 90
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-(3-(hydroxymethyl)pyrrolidin-1-yl)-5-(trifluoromethyl)benzyl)piperazine-1-carboxylate




The title compound was prepared according to the representative procedure of Example 52, using 3-bromo-5-(trifluoromethyl)benzaldehyde in Step 1, and pyrrolidin-3-ylmethanol and Ruphos in Step 2 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-(3-(hydroxymethyl)pyrrolidin-1-yl)-5-(trifluoromethyl)benzyl)piperazine-1-carboxylate as a yellow oil. 1H NMR (400 MHz, Chloroform-d) δ 6.86 (s, 1H), 6.65 (s, 2H), 5.71-5.76 (m, 1H), 3.72-3.76 (m, 2H), 3.69 (br, 4H), 3.67 (s, 2H), 3.31-3.47 (m, 3H), 3.15-3.19 (m, 1H), 2.58-2.64 (m, 1H), 2.45-2.48 (m, 4H), 2.15-2.19 (m, 1H), 1.83-1.88 (m, 1H). LCMS (ESI, m/z): 538 [M+H]+.
Example 91
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-(4-(hydroxymethyl)piperidin-1-yl)-5-(trifluoromethyl)benzyl)piperazine-1-carboxylate




The title compound was prepared according to the representative procedure of Example 52, using 3-bromo-5-(trifluoromethyl)benzaldehyde in Step 1, and piperidin-4-ylmethanol and Ruphos in Step 2 to provide 1,1,1,3,3,3-hexafluoropropan-2-yl 4-(3-(4-(hydroxymethyl)piperidin-1-yl)-5-(trifluoromethyl)benzyl)piperazine-1-carboxylate as a yellow oil. 1H NMR (400 MHz, Chloroform-d) δ 6.98-7.06 (m, 3H), 5.68-5.80 (m, 1H), 3.56-3.81 (m, 10OH), 2.76-2.84 (m, 2H), 2.50 (br, 3H), 1.86-1.91 (m, 2H), 2.69-2.76 (m, 1H), 1.33-1.46 (m, 3H). LCMS (ESI, m/z): 552 [M+H]+.
Example 92
4-(3-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)morpholine-2-carboxylic acid




The title compound was prepared according to the representative procedure of Example 77, using 3-bromo-5-(trifluoromethyl)benzaldehyde in Step 1, and methyl morpholine-2-carboxylate and Ruphos in Step 2 to provide 4-(3-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)morpholine-2-carboxylic acid as a white solid. 1H NMR (400 MHz, Chloroform-d) δ 7.12 (s, 1H), 7.00-7.06 (m, 2H), 5.69-5.78 (m, 1H), 4.22-4.24 (m, 2H), 3.82-3.89 (m, 2H), 3.60 (br, 5H), 3.41-3.44 (m, 2H), 2.93-2.95 (m, 2H), 2.56 (br, 4H). LCMS (ESI, m/z): 568 [M+H]+.
Example 93
2-(3-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)-2-oxoacetic acid




The title compound was prepared according to the representative procedure of Example 76, using 3-bromo-5-(trifluoromethyl)benzaldehyde in Step 1 to provide 2-(3-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)-2-oxoacetic acid as a white solid. 1H NMR (300 MHz, Methanol-d4) δ 8.20-8.26 (m, 2H), 7.97 (s, 1H), 6.15-6.19 (m, 1H), 3.74-3.79 (m, 2H), 3.63 (br, 4H), 2.59-2.66 (m, 4H). LCMS (ESI, m/z): 511 [M+H]+.
Example 94
1-(3-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)piperidine-4-carboxylic acid




The title compound was prepared according to the representative procedure of Example 77, using 3-bromo-5-(trifluoromethyl)benzaldehyde in Step 1, and ethyl piperidine-4-carboxylate and Ruphos in Step 2 to provide 1-(3-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)piperidine-4-carboxylic acid as a white solid. 1H NMR (300 MHz, Chloroform-d) δ 7.01-7.08 (m, 3H), 5.68-5.79 (m, 1H), 3.45-3.70 (m, 8H), 2.82-2.90 (m, 2H), 2.51 (br, 5H), 2.08-2.13 (m, 2H), 1.83-1.94 (m, 2H). LCMS (ESI, m/z): 566 [M+H]+.
Example 95
4-(2-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)morpholine-3-carboxylic acid




Step 1: Preparation of methyl 4-(3-formyl-5-(trifluoromethyl)phenyl)morpholine-3-carboxylate




The listed intermediate was prepared directly from commercially available 3-bromo-5-(trifluoromethyl)benzaldehyde according to the representative procedure of 51, Step 4, using cesium carbonate to afford 4-(3-formyl-5-(trifluoromethyl)phenyl)morpholine-3-carboxylate. LCMS (ESI, m/z): 318 [M+H]+.
Step 2: Preparation of methyl 4-(3-((4-(tert-butoxycarbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)morpholine-3-carboxylate




The listed intermediate was prepared from 4-(3-formyl-5-(trifluoromethyl)phenyl)morpholine-3-carboxylate according to the representative procedure of 39, Step 4 to afford methyl 4-(3-((4-(tert-butoxycarbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)morpholine-3-carboxylate as yellow oil. LCMS (ESI, m/z): 488 [M+H]+.
Step 3: Preparation of 4-(3-((4-(tert-butoxycarbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)morpholine-3-carboxylic acid




The title compound was prepared from 4-(3-((4-(tert-butoxycarbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)morpholine-3-carboxylate according to the representative procedure of 77, Step 2 to afford 4-(3-((4-(tert-butoxycarbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)morpholine-3-carboxylic acid as yellow oil. LCMS (ESI, m/z): 474 [M+H]+.
Step 4: Preparation of 4-(3-(piperazin-1-ylmethyl)-5-(trifluoromethyl)phenyl)morpholine-3-carboxylic acid




The title compound was prepared from 4-(3-((4-(tert-butoxycarbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)morpholine-3-carboxylic acid according to the representative procedure of 60, Step 2 to afford 4-(3-(piperazin-1-ylmethyl)-5-(trifluoromethyl)phenyl)morpholine-3-carboxylic acid as yellow oil. LCMS (ESI, m/z): 374 [M+H]+.
Step 5: Preparation of 4-(3-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)morpholine-3-carboxylic acid




The title compound was prepared from 4-(3-(piperazin-1-ylmethyl)-5-(trifluoromethyl)phenyl)morpholine-3-carboxylic acid according to the representative procedure of 39, Step 6 to afford 4-(3-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)morpholine-3-carboxylic acid as a white solid. 1H NMR (300 MHz, Chloroform-d) δ 7.14 (s, 1H), 7.06 (s, 2H), 6.10-6.18 (m, 1H), 4.39-4.43 (m, 1H), 4.34 (s, 1H), 3.89-4.04 (m, 1H), 3.85-3.88 (m, 1H), 3.48-3.75 (m, 8H), 3.39-3.43 (m, 1H), 2.38-2.50 (m, 4H). LCMS (ESI, m/z): 568 [M+H]+.
Examples 96-101 are prepared using similar procedures as outlined in the preceding examples.
Example 96
1,1,1,3,3,3-hexafluoropropan-2-yl 4-(2-(4-(1H-tetrazol-5-yl)piperidin-1-yl)-4-(trifluoromethyl)benzyl)piperazine-1-carboxylate




Example 97
4-(3-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)morpholine-3-carboxylic acid




Example 98
(R)-1-(2-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)piperidine-3-carboxylic acid




Example 99
(S)-1-(2-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)piperidine-3-carboxylic acid




Example 100
(R)-1-(3-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)piperidine-3-carboxylic acid




Example 101
(S)-1-(3-((4-(((1,1,1,3,3,3-hexafluoropropan-2-yl)oxy)carbonyl)piperazin-1-yl)methyl)-5-(trifluoromethyl)phenyl)piperidine-3-carboxylic acid




II. Biological Evaluation

Compounds are tested to assess their MAGL and serine hydrolase activity using the following in vitro and in vivo assays.
In Vitro Competitive Activity-Based Protein Profiling (Mouse).
Proteomes (mouse brain membrane fraction or cell lysates) (50 μL, 1.0 mg/mL total protein concentration) were preincubated with varying concentrations of inhibitors at 37° C. After 30 min, FP-Rh (1.0 μL, 50 μM in DMSO) was added and the mixture was incubated for another 30 min at 37° C. Reactions were quenched with SDS loading buffer (50 μL-4×) and run on SDS-PAGE. Following gel imaging, serine hydrolase activity was determined by measuring fluorescent intensity of gel bands corresponding to MAGL, ABHD6 and FAAH using ImageJ 1.43u software.
Preparation of Mouse Brain Proteomes from Inhibitor Treated Mice.
Inhibitors were administered to wild-type C57Bl/6J by oral gavage in a vehicle of polyethylene glycol. Each animal was sacrificed 4 h following administration and brain proteomes were prepared and analyzed according to previously established methods (See Niphakis, M. J., et al. (2011) ACS Chem. Neurosci. and Long, J. Z., et al. Nat. Chem. Biol. 5:37-44)
Compounds demonstrated activity in the assays described herein as indicated in the following tables (Tables 2 and 3).









TABLE 2









Mouse (IC50 value, nM)












Compd
MAGL
FAAH
ABHD6
















2
***
*
*



3
***
*
*



4
***
*
*



5
***
*
**



7
**
*
**



8
***
*
***



9
***
*
**



10
***
*
**



11
**
*
**



12
***
*
**



13
**
*
**



14
***
*
***



15
***
*
**



16
***
*
**



17
**
*
**



18
**
*
**



19
**
*
**



20
**
*
**



21
**
*
**



22
***
*
**



23
***
*
*



24
**
*
*



25
**
*
*



26
***
*
**



27
***
*
**



28
**
*
*



29
**
*
*



30
***
*
*



31
***
*
*



32
***
*
*



33
***
*
**



34
**
*
*



35
***
*
*



36
***
*
**



37
**
*
*



38
***
*
**



39
***
*
*



40
***
*
*



41
***
*
*



42
***
*
**



43
***
*
*



44
***
*
*



45
***
*
**



46
***
*
*



51
***
*
*



52
***
*
**



53
***
*
*



54
***
*
*



55
***
*
*



56
***
*
*



57
***
*
*



58
***
*
*



59
***
*
*



60
***
*
*



61
***
*
*



62
**
*
*



63
***
*
**



64
**
*
*



65
***
*
*



66
**
*
*



67
**
*
*



68
**
*
*



69
***
*
*



70
***
*
**



71
***
*
*



72
***
*
**



73
***
*
**



74
***
*
*



75
***
*
*



76
***
*
*



77
**
*
*



78
***
*
**



79
***
*
***



80
***
*
**



81
**
*
**



82
***
*
**



83
***
*
**



84
***
*
***



85
**
*
**



86
**
*
**



87
***
*
**



88
**
*
**



89
**
*
***



90
***
*
**



91
**
*
**



92
***
*
*



93
***
*
**



94
**
*
***







*** is less than 100 nM;



** is between 1000 and 100 nM;



* is greater than 1000 nM















TABLE 3








% Inhibition at 1 μM
% Inhibition at 5 mg/kg













Compd
MAGL
FAAH
ABHD6
MAGL
FAAH
ABHD6
















3



###
#
#


4
###
#
#
###
#
#


7
###
#
###





8
###
#
###





9
###
#
###





10
###
#
###





11
###
#
##





12
###
#
###





13
###
#
###





14
###
#
###





15
###
#
###





16
###
#
###
###
#
##


17
###
#
###





18
###
#
###





19
###
#
###





20
###
#
###





21
###
#
###





22
###
#
###





23
###
#
#
###
#
#


24
###
#
#





25
###
#
#





26
###
#
###
###
#
###


27
###
#
##





28
###
#
#





29
###
#
#





30



###
#
#


31
###
#
#
##
#
#


32
###
#
#
#
#
#


33
###
#
###
###
#
###


34
###
#
#





35
###
#
#
###
#
#


36
###
#
###





37
###
#
#





38
###
#
#





39
###
#
##
###
#
##


40
###
#
#
###
#
#


41
###
#
##
###
#
#


42
###
#
##
###
#
#


43
###
#
#
##
#
#


44
###
#
#
#
#
#


45
###
#
###
##
#
#


46
###
##
##





47
###
#
#





48
##
#
#





49
##
#
#





50 
#
#
#





51
###
#
#
###
#
#


52
###
#
###





53
###
#
#
###
#
#


54
###
#
#
###
#
#


55
###
#
#
###
#
#


56
###
#
#
###
#
#


57
###
#
#
###
#
#


58
###
#
#
###
#
#


59
###
#
#
###
#
#


60
###
#
#
###
#
#


61
###
#
#
###
#
#


62
###
#
#





63
###
#
###





64
###
#
#





65
###
#
##
#
#
#


66
###
#
##





67
###
#
#





68
###
#
#





69
###
#
#





70
###
#
###





71
###
#
#
###
#
#


72
###
#
###





73
###
#
###





74
###
#
#
##
#
#


75
###
#
#
#
#
#


76
###
#
###





77
###
#
###





78
###
#
###





79
###
#
###





80
###
#
##





81
###
#
###





82
###
#
###





83
###
#
#





84
###
#
###





85 
##
#
###





86
###
#
##
###
#
###


87
###
#
###





88
###
#
##





89
###
#
###





90
###
#
###





91
###
#
###





92
###
#
#





93
###
#
###





94
##
#
###





### is ≧75%;


## is between 25 and 75%;


# is ≦25

## Claims

1. A compound of Formula (I):




wherein:
R1 is halogen, —OR3, —CN, C1-6 alkyl optionally substituted by halogen, or —C(O)OR9;
R2 is —NR5R6;
R3 is selected from H, C1-6 alkyl, C1-6 haloalkyl, and C1-6 aminoalkyl;
R5 and R6, together with the nitrogen to which they are attached, form
(i) a 4-6 membered saturated monocyclic heterocycle; or
(ii) a 7-8 membered bridged heterocyclic ring optionally containing an additional O, N, or S;
wherein the 4-6 membered saturated monocyclic heterocycle is substituted with one or two substituents independently selected from —C(O)OR9; and the 4-6 membered saturated monocyclic heterocycle optionally contains an additional O, N, or S; and
the 7-8 membered bridged heterocyclic ring is optionally substituted with one or two substituents independently selected from halogen, oxo, and C1-6 alkyl; and

each R9 is independently selected from H and C1-6 alkyl;
or a solvate, hydrate, N-oxide, stereoisomer, or pharmaceutically acceptable salt thereof.

2. The compound of claim 1, or a solvate, hydrate, N-oxide, stereoisomer, or pharmaceutically acceptable salt thereof, wherein R1 is halogen, —OR3, or C1-6 alkyl optionally substituted by halogen.

3. The compound of claim 2, or a solvate, hydrate, N-oxide, stereoisomer, or pharmaceutically acceptable salt thereof, wherein R5 and R6, together with the nitrogen to which they are attached, form a 4-6 membered saturated monocyclic heterocycle, wherein:
the 4-6 membered saturated monocyclic heterocycle is substituted with one —C(O)OR9; and
the 4-6 membered saturated monocyclic heterocycle optionally contains an additional O, N, or S.

4. The compound of claim 3, or a solvate, hydrate, N-oxide, stereoisomer, or pharmaceutically acceptable salt thereof, wherein R5 and R6, together with the nitrogen to which they are attached, form a 4-6 membered saturated monocyclic heterocycle wherein:
the 4-6 membered saturated monocyclic heterocycle is substituted with one —C(O)OR9; and
the 4-6 membered saturated monocyclic heterocycle is selected from azetidine, pyrrolidine, piperidine, and morpholine.

5. The compound of claim 4, or a solvate, hydrate, N-oxide, stereoisomer, or pharmaceutically acceptable salt thereof, wherein R5 and R6, together with the nitrogen to which they are attached, form a 4-6 membered saturated monocyclic heterocycle substituted with one —C(O)OR9, wherein the 4-6 membered saturated monocyclic heterocycle is selected from pyrrolidine, piperidine, and morpholine.

6. The compound of claim 5, or a solvate, hydrate, N-oxide, stereoisomer, or pharmaceutically acceptable salt thereof, wherein R5 and R6, together with the nitrogen to which they are attached, form a 4-6 membered saturated monocyclic heterocycle substituted with one —C(O)OR9, wherein the 4-6 membered saturated monocyclic heterocycle is pyrrolidine.

7. The compound of claim 5, or a solvate, hydrate, N-oxide, stereoisomer, or pharmaceutically acceptable salt thereof, wherein R5 and R6, together with the nitrogen to which they are attached, form a 4-6 membered saturated monocyclic heterocycle substituted with one —C(O)OR9, wherein the 4-6 membered saturated monocyclic heterocycle is piperidine.

8. The compound of claim 5, or a solvate, hydrate, N-oxide, stereoisomer, or pharmaceutically acceptable salt thereof, wherein R5 and R6, together with the nitrogen to which they are attached, form a 4-6 membered saturated monocyclic heterocycle substituted with one —C(O)OR9, wherein the 4-6 membered saturated monocyclic heterocycle is morpholine.

9. The compound of claim 5, or a solvate, hydrate, N-oxide, stereoisomer, or pharmaceutically acceptable salt thereof, wherein R1 is halogen, —CH3, —CF3, —OCH3, or —OCF3.

10. The compound of claim 1, or a solvate, hydrate, N-oxide, or pharmaceutically acceptable salt thereof, wherein the compound is

11. The compound of claim 1, or a solvate, hydrate, N-oxide, or pharmaceutically acceptable salt thereof, wherein the compound is

12. The compound of claim 1, or a solvate, hydrate, N-oxide, stereoisomer, or pharmaceutically acceptable salt thereof, wherein the compound is

13. The compound of claim 1, or a solvate, hydrate, N-oxide, stereoisomer, or pharmaceutically acceptable salt thereof, wherein the compound is

14. A compound selected from:










































or a solvate, hydrate, N-oxide, stereoisomer, or a pharmaceutically acceptable salt thereof.

15. A pharmaceutical composition comprising a compound of claim 1, or a solvate, hydrate, N-oxide, stereoisomer, or a pharmaceutically acceptable salt thereof, and at least one pharmaceutically acceptable excipient.