SUPPLEMENTAL INDENTURE




 Exhibit 4.5 
  

SUPPLEMENTAL INDENTURE, dated as of September 25, 2003, among Apogent Technologies Inc., a Wisconsin corporation (together with its successors and
assigns, the “Company”), the Subsidiary Guarantors under the Indenture referred to below, and The Bank of New York, as trustee (the “Trustee”) under the Indenture, dated as of April 4, 2001, by and between the Company, the
Subsidiary Guarantors, and the Trustee, as amended and supplemented to the date hereof (as amended and supplemented, the “Indenture”). 
  
 W I T N E S S E T H : 
  
 WHEREAS, the Company, the Subsidiary Guarantors, and the Trustee have heretofore executed and delivered the Indenture providing for the issuance of 8%
Senior Notes due 2011 of the Company and related Guarantees of the Guarantors (the “Securities”); 
  
 WHEREAS, there is currently outstanding under the Indenture $325,000,000 in aggregate principal amount of the Securities; 
  
 WHEREAS, the Indenture, among other things, restricts the Company’s
ability to incur additional liens, to enter into sale leaseback transactions, and to merge or dispose of all or substantially all of its assets; 
  
 WHEREAS, Section 9.2 of the Indenture provides that the Company, the Subsidiary Guarantors and the Trustee may, with the written consent of the Holders of
at least a majority in aggregate principal amount of the Securities then outstanding (including, without limitation, consents obtained in connection with a purchase of, or tender offer or exchange offer for, the Securities), amend the Indenture;

  
 WHEREAS, the Company has offered to purchase for cash all of
the outstanding Securities upon the terms and subject to the conditions set forth in the Offer to Purchase and Consent Solicitation Statement dated September 16, 2003 and the related Consent and Letter of Transmittal, as each of them may be amended,
supplemented or modified (collectively, the “Offer”); 
  
 WHEREAS, the Offer is conditioned upon, among other things, the proposed amendments (the “Proposed Amendments”) to the Indenture and the Securities set forth herein having been approved by at least a majority in aggregate
principal amount of the outstanding Securities (and a supplemental indenture in respect thereof having been executed and delivered) with such Proposed Amendments with respect to the Securities becoming operative upon the acceptance for payment by
the Company of the Securities pursuant to the Offer (the “Acceptance”); 
  
 WHEREAS, the Company has received and delivered to the Trustee the requisite consents to effect the Proposed Amendments under the Indenture; 
  
 WHEREAS, the Company has been authorized by a resolution of its Board of Directors to enter into this Supplemental
Indenture; and 
  
 WHEREAS, all other acts and proceedings
required by law, by the Indenture and by the charter documents of the Company and the Subsidiary Guarantors to make this Supplemental Indenture a valid and binding agreement for the purposes expressed herein, in accordance with its terms, have been
duly done and performed; 
  
 NOW, THEREFORE, in consideration of
the premises and the covenants and agreements contained herein, and for other good and valuable consideration the receipt of which is hereby acknowledged, and for the equal and proportionate benefit of the Holders of the Securities, the Company, the
Subsidiary Guarantors, and the Trustee hereby agree as follows: 
  
 ARTICLE ONE 
  

	Section	 	1.01    Definitions. 

  
 Capitalized terms used in this Supplemental Indenture and not otherwise defined herein shall have the meanings assigned to such terms in the Indenture.









 ARTICLE TWO 

	Section	 	2.01    Amendment of Section 1.1. 

  
 Effective upon, and subject only to, the Acceptance, the provisions of Section 1.1 of the Indenture are amended by: 
  
 (a) deleting the following definitions: “Attributable
Indebtedness”; “Consolidated Net Tangible Assets”; “GAAP”; “Lien”; “Permitted Liens”; “Principal Property”; and “Sale-Leaseback Transaction.” 
  

	Section	 	2.02    Amendment of Section 1.2. 

  
 Effective upon, and subject only to, the Acceptance, the provisions of Section 1.2 of the Indenture are amended by deleting the references to the term
“Funded Debt.” 
  

	Section	 	2.03    Amendment of Section 3.2. 

  
 Effective upon, and subject only to, the Acceptance, the provisions of Section 3.2 of the Indenture are amended by deleting the text of such Section in
its entirety and inserting in lieu thereof the phrase “[intentionally omitted]”. 
  

	Section	 	2.04    Amendment of Section 3.3. 

  
 Effective upon, and subject only to, the Acceptance, the provisions of Section 3.3 of the Indenture are amended by deleting the text of such Section in
its entirety and inserting in lieu thereof the phrase “[intentionally omitted]”. 
  

	Section	 	2.05    Amendment of Section 3.4. 

  
 Effective upon, and subject only to, the Acceptance, the provisions of Section 3.4 of the Indenture are amended by deleting the text of such Section in
its entirety and inserting in lieu thereof the phrase “[intentionally omitted]”. 
  

	Section	 	2.06    Amendment of Section 3.6. 

  
 Effective upon, and subject only to, the Acceptance, the provisions of Section 3.6 of the Indenture are amended by deleting the text of such Section in
its entirety and inserting in lieu thereof the phrase “[intentionally omitted]”. 
  

	Section	 	2.07    Amendment of Section 3.7. 

  
 Effective upon, and subject only to, the Acceptance, the provisions of Section 3.7 of the Indenture are amended by deleting the text of such Section in
its entirety and inserting in lieu thereof the phrase “[intentionally omitted]”. 
  

	Section	 	2.08    Amendment of Section 3.8. 

  
 Effective upon, and subject only to, the Acceptance, the provisions of Section 3.8 of the Indenture are amended by deleting the text of such Section in
its entirety and inserting in lieu thereof the phrase “[intentionally omitted]”. 
  
  

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	Section	 	2.09    Amendment of Section 3.9. 

  
 Effective upon, and subject only to, the Acceptance, the provisions of Section 3.9 of the Indenture are amended by deleting the text of such Section in
its entirety and inserting in lieu thereof the phrase “[intentionally omitted]”. 
  

	Section	 	2.10    Amendment of Section 4.1. 

  
 Effective upon, and subject only to, the Acceptance, the provisions of Section 4.1 of the Indenture are amended by deleting the text of such Section in
its entirety and inserting in lieu thereof the phrase “[intentionally omitted]”. 
  

	Section	 	2.11    Amendment of Section 6.1. 

  
 Effective upon, and subject only to, the Acceptance, the provisions of Section 6.1 of the Indenture are amended by deleting the text of paragraphs (4),
(5), and (6) of such Section of the Indenture and the corresponding provisions of the Securities in their entirety and inserting in lieu thereof the phrase “[intentionally omitted]”. 
  
 ARTICLE THREE 
  

	Section	 	3.01    Waiver; Continuing Effect of Indenture. 

  
 If and to the extent that any of the restrictive covenants in the Indenture would prohibit or restrict the tender offer reflected in the Offer, those
restrictions are hereby waived. 
  
 Except as expressly provided
herein, all of the terms, provisions and conditions of the Indenture and the Securities outstanding thereunder shall remain in full force and effect. 
  

	Section	 	3.02    Construction of Supplemental Indenture. 

  
 This Supplemental Indenture is executed as and shall constitute an indenture supplemental to the Indenture and shall be construed in connection with and
as part of the Indenture. This Supplemental Indenture shall be governed by and construed in accordance with the laws of the State of New York. 
  

	Section	 	3.03    Trust Indenture Act Controls. 

  
 If any provision of this Supplemental Indenture limits, qualifies or conflicts with another provision of this Supplemental Indenture or the Indenture that
is required to be included by the Trust Indenture Act of 1939, as amended, as in force at the date this Supplemental Indenture is executed, the provision required by said Act shall control. 
  

	Section	 	3.04    Trustee Disclaimer. 

  
 The recitals contained in this Supplemental Indenture shall be taken as the statements of the Company, and the Trustee assumes no responsibility for their
correctness. The Trustee makes no representations as to the validity or sufficiency of this Supplemental Indenture. 
  

	Section	 	3.05    Counterparts. 

  
 This Supplemental Indenture may be executed in any number of counterparts, each of which so executed shall be deemed to be an original, but all such
counterparts shall together constitute but one and the same instrument. 
  

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 Section 3.06    Effective Date; Operative Date. 
  
 This Supplemental Indenture will become effective upon execution and delivery
by the Company, the Subsidiary Guarantors and the Trustee, provided that it will not become operative until the first settlement date as provided in the Offer. 
  

[Signature pages follow.] 
  

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 IN WITNESS WHEREOF, the parties hereto have caused this Supplemental Indenture to be duly executed as of
the day and year first above written. 
  
  

	APOGENT TECHNOLOGIES INC.
		
	 By:
	 	 /s/    MICHAEL K.
BRESSON        


	 	 	 Michael K. Bresson,
 Executive Vice
President – General Counsel
 and Secretary



  

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	 ABGENE INC.
 APOGENT FINANCE COMPANY
 APOGENT TRANSITION CORP.
 BARNSTEAD THERMOLYNE CORPORATION
 BT CANADA HOLDINGS INC.
 CAPITOL VIAL, INC.
 CHASE SCIENTIFIC GLASS, INC.
 CONSOLIDATED TECHNOLOGIES, INC.
 ERIE SCIENTIFIC COMPANY
 ERIE SCIENTIFIC COMPANY OF PUERTO RICO
 ERIE UK HOLDING COMPANY
 EVER READY THERMOMETER CO., INC.
 GENEVAC INC.
 G&P LABWARE HOLDINGS INC.
 LAB-LINE INSTRUMENTS, INC.
 LAB VISION CORPORATION
 MATRIX TECHNOLOGIES CORPORATION
 MICROGENICS CORPORATION
 MOLECULAR BIOPRODUCTS, INC.
 NALGE NUNC INTERNATIONAL CORPORATION
 NATIONAL SCIENTIFIC COMPANY
 THE NAUGATUCK GLASS COMPANY
 NEOMARKERS, INC.
 NERL DIAGNOSTICS CORPORATION
 OWL SEPARATION SYSTEMS, INC.
 QUALITY SCIENTIFIC PLASTICS, INC.
 REMEL INC.
 RICHARD-ALLAN SCIENTIFIC COMPANY
 ROBBINS SCIENTIFIC CORPORATION
 SAMCO SCIENTIFIC CORPORATION
 SEPARATION TECHNOLOGY, INC.
 SERADYN INC.

		
	 By:
	 	 /s/    MICHAEL K.
BRESSON        


	 	 	 Michael K. Bresson,
 Vice President and
Secretary



  

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	 APOGENT HOLDING COMPANY
 APOGENT
SERVICE CORPORATION

		
	 By:
	 	 /s/    MICHAEL K.
BRESSON        


	 	 	 Michael K. Bresson,
 President and
Secretary



  
  

	METAVAC LLC
		
	By:	 	 The Naugatuck Glass Company
 Sole Member and
Manager

		
	 By:
	 	 /s/    MICHAEL K.
BRESSON        


	 	 	 Michael K. Bresson,
 Vice President and
Secretary



  
  

	THE BANK OF NEW YORK, as Trustee
		
	 By:
	 	 /s/    KISHA A.
HOLDER        


	 	 	 Kisha A. Holder
 Assistant Vice
President



  

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