# Patent

## Title
Method for treating amyloid disease

## Abstract

The invention relates to methods for treating human amyloid disease by administration of modified Aβ peptide immunogens.

## Background
CROSS REFERENCE TO RELATED APPLICATIONS
This application is a divisional of U.S. patent application Ser. No. 14/559,301 filed on Dec. 3, 2014, now U.S. Pat. No. 9,295,719, which is a divisional of U.S. patent application Ser. No. 13/553,566 filed on Jul. 19, 2012, now U.S. Pat. No. 8,906,382, which claims priority from U.S. Provisional Patent Application No. 61/509,442 filed on Jul. 19, 2011, the entire contents of each of which applications are herein incorporated by reference.
SEQUENCE LISTING
The instant application contains a Sequence Listing in ASCII format that was entered in this application on Mar. 25, 2016. Said sequence listing is hereby incorporated by reference in its entirety. Said ASCII copy was entered on Mar. 25, 2016, is named US15076122.txt, and is 3.85 kilobytes in size.


STATEMENT AS TO FEDERALLY SPONSORED RESEARCH
This invention was made with government support under Grant Nos. NS073502 and AG20245 awarded by the National Institutes of Health. The government has certain rights in the invention.


FIELD OF THE INVENTION
The present invention relates to methods for treating amyloid diseases. Specifically, this invention relates to methods of reducing the levels of amyloid-beta (Aβ) peptides in bodily fluids by administration of Aβ peptide immunogens capable of eliciting antibodies directed against Aβ.
BACKGROUND OF THE INVENTION
Amyloid diseases (disorders of protein folding), or amyloidoses, are characterized by the accumulation of a peptide, including the Aβ peptide, existing as abnormal insoluble cross-β sheet fibrils or amyloid deposits in the affected organs. Amyloid diseases include, but are not limited to, Alzheimer's disease, Lewy body dementia, type 2 diabetes, Huntington's disease, Parkinson's disease, and chronic inflammation. Amyloidosis is also a common and serious complication of long-term hemodialysis for end-stage renal failure. Amyloidosis—in which amyloid deposits are the direct cause of death is responsible for about one per thousand of all deaths in developed countries.
Alzheimer's disease (AD) is the most common form of late-life dementia in adults, constituting the sixth leading cause of death in the United States (see, e.g., Alzheimer's Association, 2012 Alzheimer's Disease Facts and Figures, Alzheimer's & Dementia, Volume 8, Issue 2, available at www.alz.org/downloads/facts_figures_2012.pdf). AD is also globally the most common cause of dementia; the increasing numbers of AD patients worldwide is one of the most pressing medical issues of the 21st century (see, e.g., Alzheimer's Disease International World Alzheimer Report 2011, The benefits of early diagnosis and intervention, Martin Prince, Renata Bryce and Cleusa Ferri, Institute of Psychiatry, King's College London, UK, published by Alzheimer's Disease International (ADI), September 2011, available at www.alz.co.uk/research/world-report-2011). Approximately 5% of the population over 65 years old is affected by this progressive degenerative disorder that is characterized by memory loss, confusion and a variety of cognitive disabilities.
Neuropathologically, AD is characterized by four major lesions: a) intraneuronal, cytoplasmic deposits of neurofibrillary tangles (NFT), b) parenchymal amyloid deposits called neuritic plaques, c) cerebrovascular amyloidosis, and d) synaptic and neuronal loss. One of the key events in AD is the deposition of amyloid as insoluble fibrous masses (amyloidogenesis) resulting in extracellular neuritic plaques and deposits around the walls of cerebral blood vessels. The major constituent of the neuritic plaques and congophilic angiopathy is Aβ, although these deposits also contain other proteins such as glycosaminoglycans and apolipoproteins. The major constituent of NFTs is the tau protein in an abnormal conformation which is hyperphosphorylated. NFTs are also considered amyloid deposits, wherein tau (pTau) is present in toxic oligomeric forms that might or might not be soluble. Hence, in AD two different biochemical types of proteins are deposited and at least two major protein toxic oligomeric forms are involved.
Aβ is a 4.14.3 kD hydrophobic peptide that is encoded on chromosome 21 as part of a much longer amyloid precursor protein APP (Muller-Hill et al., Nucleic Acids Res 1989; 17:517-522). The APP protein starts with a leader sequence (signal peptide), followed by a cysteine-rich region, an acidic-rich domain, a protease inhibitor motif, a putative N-glycosylated region, a transmembrane domain, and finally a small cytoplasmic region. The Aβ sequence begins close to the membrane on the extracellular side and ends within the membrane. Two-thirds of Aβ faces the extracellular space, and the other third is embedded in the membrane (Kang et al., Nature 1984; 325:733-736, Dyrks et al., EMBO J 1988; 7:949-957). Several lines of evidence suggest that amyloid may play a central role in the early pathogenesis of AD (Soto et al., 1994; 63:1191-1198).
Evidence that amyloid may play an important role in the early pathogenesis of AD comes primarily from studies of individuals affected by the familial form of AD (FAD) or by Down's syndrome. Down's syndrome patients have three copies of the APP gene and develop AD neuropathology at an early age (Wisniewski et al., Ann Neurol 1985; 17:278-282). Genetic analysis of families with hereditary AD revealed mutations in chromosome 21, near or within the Aβ sequence (Ghiso et al., Adv. Drug Deliv. Rev. 2002; 54(12):1539-51), in addition to mutations within the presenilin 1 and 2 genes. Moreover, it was reported that transgenic mice expressing high levels of human mutant APP progressively develop amyloidosis in their brains (Games et al., Nature 1995; 373:523-527). These findings appear to implicate amyloidogenesis in the pathophysiology of AD. In addition, Aβ fibrils are toxic to neurons in culture, and to some extent when injected into animal brains (Sigurdsson et al., Neurobiol Aging 1996; 17:893-901; Sigurdsson et at, J Neuropathol Exp Neurol. 1997; 56:714-725).
Furthermore, several other pieces of evidence suggest that the deposition of Aβ is a central triggering event in the pathogenesis of AD, which subsequently might be linked or related to NFT formation and neuronal loss. The amyloid deposits in AD share a number of properties with all the other cerebral amyloidoses, such as the prion related amyloidoses, as well as the systemic amyloidoses. These characteristics are: 1) being relatively insoluble; 2) having a high degree of β-sheet secondary structure, which is associated with a tendency to aggregate or polymerize; 3) ultrastructurally, the deposits are mainly fibrillary; 4) the presence of certain amyloid-associating proteins such as amyloid P component, proteoglycans and apolipoproteins; and 5) deposits show a characteristic apple-green birefringence when viewed under polarized light after Congo red staining.
The same peptide that forms amyloid deposits in the AD brain was also found in a soluble form (sAβ) normally circulating in human body fluids (Seubert et al., Nature 1992; 359:325-327; Shoji et al., Science 1992; 258:126-129). sAβ was reported to pass freely from the brain to the blood (Ji et al., Journal of Alzheimer's Disease 2001; 3:23-30; Shibata et al., J Clin Invest 2000; 106: 1489-99; Ghersi-Egea et al., J Neurochem 1996; 67(2):880-3; Zlokovic et al., Biochem Biophys Res Commun 1994; 205:1431-1437), reported that the blood-brain barrier (BBB) has the capability to control cerebrovascular sequestration and transport of circulating sAβ, and that the transport of sAβ across the BBB was significantly increased in guinea pigs when sAβ was perfused as a complex with apolipoprotein J (apoJ). The sAβ-apoJ complex was found in normal cerebrospinal fluid (CSF; Ghiso et al., Biochem J 1993; 293:27-30; Ghiso et al., Mol Neurobiol 1994; 8:49-64) and in vivo studies indicated that sAβ is transported with apoJ as a component of the high density lipoproteins (HDL) in normal human plasma (Koudinov et al., Biochem Biophys Res Commun 1994; 205:1164-1171). It was also reported by (Zlokovic et al., Proc Natl Acad Sci USA 1996; 93:4229-4234), that the transport of sAβ from the circulation into the brain was almost abolished when the apoJ receptor, gp330, was blocked. It has been suggested that the amyloid formation is a nucleation-dependent phenomena in which the initial insoluble “seed” allows the selective deposition of amyloid (Jarrett et al., Cell 1993; 73:1055-1058; Jarrett et al., Biochemistry 1993; 32:4693-4697).
Therapeutic strategies proposed for treating AD and other amyloid diseases include the use of compounds that affect processing of the amyloid-β precursor protein (Dovey et al., J Neurochem. 2001; 76:173-182), or that interfere with fibril formation or promote fibril disassembly (Soto et al., Nat Med 1998; 4:822-826; Sigurdsson et al., J Neuropath Exp Neurol 2000; 59:11-17; Findeis M A., Biochim Biophys Acta 2000; 1502:76-84), as well as the administration of Aβ antibodies to disassemble fibrillar Aβ, maintain Aβ solubility and to block the toxic effects of Aβ (Frenkel et al, J Neuroimmunol 1999; 95:136-142). However, recently a Phase II clinical trial using a vaccination approach where Aβ1-42 was injected into individuals in the early stages of AD was terminated because of cerebral inflammation observed in some patients.
Taken together, despite some advances in the art, to date, there is no cure or effective therapy for reducing a patient's amyloid burden or preventing amyloid deposition in AD and other amyloid diseases. Thus, there exists a need in the art for developing effective methods for reducing a patient's amyloid burden.
SUMMARY OF THE INVENTION
The present invention is based, in part, on the discovery that amyloid diseases can be treated by the administration of immunogenic modified Aβ peptides that elicit antibodies directed against a particular conformation of Aβ in order to bind to Aβ in organs (e.g., brain) and/or bodily fluids.
Although the primary sequence of Aβ and tau has no homology, the abnormal conformation of both these proteins when deposited as amyloid plaques and NFTs or in soluble and semi-soluble Aβ oligomers, is similar. Hence, the present inventors have further hypothesized that a treatment that is directed against the abnormal conformation of Aβ in amyloid plaques and/or in soluble and semi-soluble Aβ oligomers may also directly reduce NFT-related pathology.
The administration of immunogenic modified Aβ peptides of the present invention leads to the reduction of a patient's amyloid burden and the reduction in Aβ and/or pTau oligomers in bodily fluids and/or affected organs.
Thus, in one aspect, the invention provides immunogenic modified Aβ peptides, which peptides are soluble, non-fibrillogenic, contain Lysine substitutions in amino acid positions corresponding to amino acids 18 and 19 of Aβ1-42, and have the property of eliciting antibodies directed against the abnormal conformation of Aβ in amyloid plaques and/or in soluble and semi-soluble Aβ oligomers.
The present invention also provides various conjugated and fusion forms of said immunogenic modified Aβ peptides, which conjugated and fusion forms are capable of eliciting antibodies directed against the abnormal conformation of Aβ in amyloid plaques and/or in soluble and semi-soluble Aβ oligomers.
The invention also provides polymerized forms of said immunogenic modified Aβ peptides, which polymerized forms have molecular weight less than 100 kDa and are capable of eliciting antibodies directed against the abnormal conformation of Aβ in amyloid plaques and/or in soluble and semi-soluble Aβ oligomers. Such polymerized forms can be produced using methods known in the art (such as, e.g., by a reaction with a cross linking reagent [e.g., glutaraldehyde or 1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC)] or by cysteine oxidation-induced disulfide cross-linking). In one embodiment, such polymerized forms are produced using controlled polymerization with glutaraldehyde as described in U.S. Patent Appl. Publ. No. 2010/0284909 and Goni et al., PLoS One, 2010, 5(10): e13391.
Non-limiting examples of the immunogenic modified Aβ peptides of the present invention include Aβ 1-30 K18K19 (DAEFRHDSGYEVHHQKLKKFAEDVGSNKGA; SEQ ID NO: 1), Aβ 1-40 K18K19 (DAEFRHDSGYEVHHQKLKKFAEDVGSNKGAIIGLMVGGVV; SEQ ID NO: 2), Aβ 1-42 K18K19 (DAEFRHDSGYEVHHQKLKKFAEDVGSNKGAIIGLMVGGVVIA; SEQ ID NO: 3), Aβ 1-20 K18K19 (DAEFRHDSGYEVHHQKLKKF; SEQ ID NO: 4), Aβ 10-30 K18K19 (YEVHHQKLKKFAEDVGSNKGA; SEQ ID NO: 5), Aβ 10-40 K18K19 (YEVHHQKLKKFAEDVGSNKGAIIGLMVGGVV; SEQ ID NO: 6), and Aβ 10-42 K18K19 (YEVHHQKLKKFAEDVGSNKGAIIGLMVGGVVIA; SEQ ID NO: 7).
The invention also provides pharmaceutical compositions comprising one or more of the immunogenic modified Aβ peptides and/or polymerized forms of the invention and a pharmaceutically acceptable carrier or diluent. In one embodiment, the pharmaceutical composition further comprises an adjuvant. In another embodiment, the pharmaceutical composition further comprises a polymerized British amyloidosis related peptide (pABri) (e.g., as described in U.S. Patent Appl. Publ. No. 2010/0284909 and Goni et al., PLoS One, 2010, 5(10): e13391). In one embodiment, the monomer of the pABri peptide has the sequence CSRTVKKNIIEEN (SEQ ID NO: 8).
In conjunction with the immunogenic modified Aβ peptides and pharmaceutical compositions, the present invention also provides a method of treating a patient (e.g., human) suffering from an amyloid disease (e.g., Alzheimer's disease, Lewy Body Dementia, Frontotemporal dementia, type 2 diabetes, Huntington's disease, Parkinson's disease, amyloidosis associated with hemodialysis for renal failure, Down syndrome, hereditary cerebral hemorrhage with amyloidosis, kuru, Creutzfeldt-Jakob disease, Gerstmann-Straussler-Scheinker disease, fatal familial insomnia, British familial dementia, Danish familial dementia, familial corneal amyloidosis, Familial corneal dystrophies, medullary thyroid carcinoma, insulinoma, isolated atrial amyloidosis, pituitary amyloidosis, aortic amyloidosis, plasma cell disorders, familial amyloidosis, senile cardiac amyloidosis, inflammation-associated amyloidosis, familial Mediterranean fever, systemic amyloidosis, and familial systemic amyloidosis) or a tauopathy (e.g., Frontotemporal dementia, chronic traumatic encephalopathy, progressive supranuclear palsy, corticobasal degeneration) comprising administering to the patient a therapeutically effective amount of one or more peptides (and/or polymerized forms thereof) of the invention (or administering a pharmaceutical composition comprising such peptide(s) and/or polymerized forms thereof). In one specific embodiment, the peptide is Aβ 1-30 K18K19 (SEQ ID NO: 1). In another specific embodiment, two or more different peptides and/or polymerized forms thereof are administered. In one specific embodiment, the peptide(s) of the invention (or polymerized forms thereof) is administered in combination with a polymerized British amyloidosis related peptide (pABri). Useful pABri peptides are described, for example, in U.S. Patent Appl. Publ. No. 2010/0284909 and Goni et al., PLoS One, 2010, 5(10): e13391. In one embodiment, the monomer of the pABri peptide has the sequence CSRTVKKNIIEEN (SEQ ID NO: 8).
In one embodiment, the treatment method of the invention further comprises administering an adjuvant.
In one embodiment, the peptide of the invention (or polymerized forms thereof) is administered systemically (e.g., intramuscularly, subcutaneously, orally, or intranasally).
In one embodiment, the therapeutically effective amount of the peptide (or polymerized forms thereof) is between about 0.1 mg and about 20 mg per kg body weight of the patient per day.
In one embodiment, the patient's blood-brain-barrier (BBB) is permeabilized prior to administration of the peptide (e.g., by administering insulin growth factor I (IGF-I)).
These and other aspects of the present invention will be apparent to those of ordinary skin in the art in the following description, claims and drawings.



BRIEF DESCRIPTION OF THE DRAWINGS
FIGS. 1A-1D are graphs showing the antibody titers of IgM and IgG cross-reactive with Aβ1-40 and Aβ1-42 at T0, T1 (after the 6th inoculation) and Tf (at the time of sacrifice) (***p<0.001; **p<0.01; *p>0.05 versus T0) of TgSwDI (A) and 3×Tg (B-D) mice immunized with Aβ 1-30 K18K19 peptide (Aβ1-30KK) (SEQ ID NO: 1) (A, B), vehicle control (C), or a combination of Aβ1-30KK and polymerized British amyloidosis related peptides (pABri) (monomer sequence SEQ ID NO: 8) (D).
FIGS. 2A-2B are graphs showing the results of the Radial Arm Maze behavioral testing in 3×Tg (A) and TgSwDI (B) mice immunized with Aβ 1-30 K18K19 peptide (Aβ1-30KK) (SEQ ID NO: 1), vehicle control, polymerized British amyloidosis related peptides (pABri) (monomer sequence SEQ ID NO: 8), or a combination of Aβ1-30KK and pABri.
FIG. 3 is a graph showing immunohistological analysis of the hippocampal amyloid burden in TgSwDI mice immunized with Aβ 1-30 K18K19 peptide (Aβ1-30KK) (SEQ ID NO: 1), Aβ 1-42 peptide (SEQ ID NO: 9), vehicle control, or polymerized British amyloidosis related peptides (pABri) (monomer sequence SEQ ID NO: 8).
FIGS. 4A-C are bar graphs showing immunohistological analysis in 3×Tg mice immunized with Aβ 1-30 K18K19 peptide (Aβ1-30KK) (SEQ ID NO: 1), vehicle control, polymerized British amyloidosis related peptides (pABri) (monomer sequence SEQ ID NO: 8), or a combination of Aβ1-30KK and pABri. Panels A and B show the PHF-1 immunohistochemical quantitation in the cortex (A) and CA1 hippocampus (B). Panel C shows the amyloid burden in the hippocampus. All treated mice demonstrate a clear reduction in the amyloid burden compared to controls.
FIG. 5 shows an immunoblot developed with antibody PHF-1 which detects Tau phosphorylated at Ser396/Ser404 in soluble brain homogenates of 3×Tg mice immunized with Aβ 1-30 K18K19 peptide (Aβ1-30KK) (SEQ ID NO: 1), vehicle control, polymerized British amyloidosis related peptides (pABri) (monomer sequence SEQ ID NO: 8), or a combination of Aβ1-30KK and pABri. All treatment groups showed a marked reduction in the band at 65 kDa when compared to the Group 3 (controls inoculated with vehicle). The same result was repeated in all the animals of each group (n=8).
FIG. 6 is a bar graph showing the reduction, compared to controls, in soluble Aβ1-40 (SEQ ID NO: 10) and Aβ1-42 (SEQ ID NO: 9) in brain homogenates from 3×Tg mice treated with Aβ 1-30 K18K19 peptide (Aβ1-30KK) (SEQ ID NO: 1), pABri (monomer sequence SEQ ID NO: 8) or combined Aβ1-30KK with pABri.



DETAILED DESCRIPTION OF THE INVENTION
The present invention provides a method for removing deposited, oligomeric and soluble amyloid β, including Aβ1-40 and Aβ1-42 from bodily fluids and organs. The present invention also provides a method for removing toxic pTau oligomers in an abnormal conformation from bodily fluids and organs.
The efficacy of the treatment of the invention can be determined by evaluating the Alzheimer's Disease (AD) symptoms of the patient and/or by measuring the Aβ concentration in bodily fluids or organs (e.g., the patient's blood). Blood Aβ peptide measurements can be conducted using any method known in the art. For example, blood Aβ peptide measurements can involve taking a blood sample, separating serum from red blood cells, and using radioimmunoassay, enzyme linked immunosorbent assay (ELISA) or chromatographic analysis of the Aβ peptide contents in serum.
The subject or patient to which the present invention may be applicable can be any vertebrate species, preferably mammalian, including but not limited to, cows, horses, sheep, pigs, fowl (e.g., chickens), goats, cats, dogs, hamsters, mice, rats, rabbits, monkeys, chimpanzees, and humans. In a preferred embodiment, the subject is a human. The invention is particularly applicable for human subjects at risk for or suffering from Alzheimer's Disease (AD). However, the invention is also applicable for treatment of other amyloid diseases (such as, e.g., Lewy Body Dementia, Frontotemporal dementia, type 2 diabetes, Huntington's disease, Parkinson's disease, amyloidosis associated with hemodialysis for renal failure, Down syndrome, hereditary cerebral hemorrhage with amyloidosis, kuru, Creutzfeldt-Jakob disease, Gerstmann-Straussler-Scheinker disease, fatal familial insomnia, British familial dementia, Danish familial dementia, familial corneal amyloidosis, Familial corneal dystrophies, medullary thyroid carcinoma, insulinoma, isolated atrial amyloidosis, pituitary amyloidosis, aortic amyloidosis, plasma cell disorders, familial amyloidosis, senile cardiac amyloidosis, inflammation-associated amyloidosis, familial Mediterranean fever, systemic amyloidosis, and familial systemic amyloidosis) or a tauopathy (e.g., Frontotemporal dementia, chronic traumatic encephalopathy, progressive supranuclear palsy, corticobasal degeneration).
As used herein, “treat” or “treatment” generally refers to a reduction in the concentration or amount of Aβ peptide or toxic pTau oligomers in a bodily fluid and/or organ, including, but not limited to, the administration of peptides capable of inducing an immune response in vivo directed against deposited, oligomeric and/or soluble amyloid β (Aβ, including Aβ1-40 and Aβ1-42) and/or toxic pTau oligomers in bodily fluids and/or organs. “Treatment” also includes prophylactic treatment to those at risk for amyloid diseases, e.g., familial AD.
The term “bodily fluid” as used herein includes blood, plasma, serum, cerebroventricular fluid (CVF), cerebrospinal fluid, and other extracellular or interstitial fluids in the body of a subject.
The term “about” or “approximately” means within an acceptable error range for the particular value as determined by one of ordinary skill in the art, which will depend in part on how the value is measured or determined, i.e., the limitations of the measurement system. For example, “about” can mean within 1 or more than 1 standard deviations, per the practice in the art. Alternatively, “about” can mean a range of up to 20%, preferably up to 10%, more preferably up to 5%, and more preferably still up to 1% of a given value. Alternatively, particularly with respect to biological systems or processes, the tern can mean within an order of magnitude, preferably within 5-fold, and more preferably within 2-fold, of a value.
In accordance with the present invention there may be employed conventional molecular biology, microbiology, and recombinant DNA techniques within the skill of the art. Such techniques are explained fully in the literature. See, e.g., Sambrook, Fritsch & Maniatis, Molecular Cloning: A Laboratory Manual, Second Edition (1989) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (herein “Sambrook et al., 1989”); DNA Cloning: A practical Approach, Volumes I and II (D. N. Glover ed. 1985); Oligonucleotide Synthesis (M J. Gait ed. 1984); Nucleic Acid Hybridization (B. D. Hames & S. J. Higgins eds. (1985>>; Transcription and Translation (B. D. Hames & S. J. Higgins, eds. (1984>>; Animal Cell Culture (R. I. Freshney, ed. (1986>>; Immobilized Cells and Enzymes (IRL Press, (1986>>; B. Perbal, A practical Guide To Molecular Cloning (1984); F. M. Ausubel et al. (eds.), Current Protocols in Molecular Biology, John Wiley & Sons, Inc. (1994).
As used herein, the term “isolated” means that the referenced material is removed from its native environment, e.g., a cell. Thus, an isolated biological material can be free of some or all cellular components (e.g., cytoplasmic or membrane component). A material shall be deemed isolated if it is present in a cell extract or in a heterologous cell. An isolated material may be, but need not be, purified.
The term “purified” as used herein refers to material that has been isolated under conditions that reduce or eliminate the presence of unrelated materials, i.e., contaminants, including native materials from which the material is obtained. For example, a purified protein is preferably substantially free of other proteins or nucleic acids with which it is associated in a cell; a purified nucleic acid molecule is preferably substantially free of proteins or other unrelated nucleic acid molecules with which it can be found within a cell. As used herein, the term “substantially free” is used operationally, in the context of analytical testing of the material. Preferably, purified material substantially free of contaminants is at least 50% pure; more preferably. at least 90% pure, and more preferably still at least 99% pure. Purity can be evaluated, e.g., by chromatography, gel electrophoresis, immunoassay, composition analysis, biological assay, and other methods known in the art.
Methods for purification are well-known in the art. For example, nucleic acids can be purified by precipitation, chromatography (including, without limitation, preparative solid phase chromatography, oligonucleotide hybridization, and triple helix chromatography), ultracentrifugation, and other means. Peptides and proteins can be purified by various methods including, without limitation, preparative disc-gel electrophoresis and isoelectric focusing; affinity HPLC, reversed-phase HPLC, gel filtration or size exclusion, ion exchange and partition chromatography; precipitation and salting-out chromatography; extraction; and countercurrent distribution. For some purposes, it is preferable to produce a peptide or protein in a recombinant system in which said peptide or protein may contain an additional sequence tag that facilitates purification, such as, but not limited to, a polyhistidine sequence, or a sequence that specifically binds to an antibody, such as FLAG and GST. The resulting tagged peptide or protein can then be purified from a crude lysate of the host cell by chromatography on an appropriate solid-phase matrix. Alternatively, antibodies produced against the peptide or protein (or against peptides derived therefrom) can be used as purification reagents. Cells can be purified by various techniques, including centrifugation, matrix separation (e.g., nylon wool separation), panning and other immunoselection techniques, depletion (e.g., complement depletion of contaminating cells), and cell sorting (e.g., fluorescence activated cell sorting (FAS)). Other purification methods are possible and contemplated herein. A purified material may contain less than about 50%, preferably less than about 75%, and most preferably less than about 90%, of the cellular components, media, proteins, or other nondesimble components or impurities (as context requires), with which it was originally associated. The term “substantially pure” indicates the highest degree of purity which can be achieved using conventional purification techniques known in the art.
Immunigenic Modified Amyloid Beta (Aβ) Peptides of the Invention
Non-limiting examples of immunogenic modified Aβ peptides for use in the present invention are as follows:







TABLE 1







List of Exemplified Peptides of the Invention










SEQ




ID



Peptide Name
NO
Sequence





Aβ 1-30 K18K19
1
DAEFRHDSGYEVHHQKLKKFAEDVGSNKGA


Aβ 1-40 K18K19
2
DAEFRHDSGYEVHHQKLKKFAEDVGSNKGAIIGLMVGGVV


Aβ 1-42 K18K19
3
DAEFRHDSGYEVHHQKLKKFAEDVGSNKGAIIGLMVGGVVIA


Aβ 1-20 K18K19
4
DAEFRHDSGYEVHHQKLKKF


Aβ 10-30 K18K19
5
YEVHHQKLKKFAEDVGSNKGA


Aβ 10-40 K18K19
6
YEVHHQKLKKFAEDVGSNKGAIIGLMVGGVV


Aβ 10-42 K18K19
7
YEVHHQKLKKFAEDVGSNKGAIIGLMVGGVVIA









The present invention also encompasses various additional immunogenic modified Aβ peptides having substitutions in amino acid positions corresponding to amino acids 18 and 19 of Aβ1-42, which peptides are soluble non-fibrillogenic and are capable of eliciting antibodies directed against the abnormal conformation of Aβ in amyloid plaques and/or in soluble and semi-soluble Aβ oligomers.
The immunogenic modified Aβ peptides of the invention can be synthesized using techniques well known to those of ordinary skill in the art (see, e.g., SYNTHETIC PEPTIDES: A USERS GUIDE 93-210 (Gregory A. Grant ed., 1992)) or can be purchased from numerous commercial sources such as, e.g., the W. M. Keck Biotechnology Resource Laboratory, Yale University, 300 George Street, New Haven, Conn. 06511.
To enhance immunogenicity, the peptide of the invention can be linked in-frame to an adjuvant polypeptide. The adjuvant polypeptide can be any adjuvant polypeptide known in the art, including, but not limited to, cholera toxin B, flagellin, human papillomavirus L1 or L2 protein, herpes simplex glycoprotein D (gD), complement C4 binding protein, TL4 ligand, and IL-1.beta. The peptide may be linked directly to the adjuvant polypeptide or coupled to the adjuvant by way of a short linker sequence. Suitable linker sequences include glycine or serine-rich linkers described supra or other flexible immunoglobulin linkers as disclosed, e.g., in U.S. Pat. No. 5,516,637.
Alternatively or in addition, the peptide of the invention can be conjugated to an immunogenic carrier molecule. The immunogenic carrier molecule can be covalently or non-covalently bonded to the peptide. Suitable immunogenic carrier molecules include, but are not limited to, serum albumins, chicken egg ovalbumin, keyhole limpet hemocyanin, tetanus toxoid, thyroglobulin, pneumococcal capsular polysaccharides, CRM 197, immunoglobulin molecules, attenuated Salmonella, and meningococcal outer membrane proteins. Other suitable immunogenic carrier molecules include T-cell epitopes, such as tetanus toxoid (e.g., the P2 and P30 epitopes), Hepatitis B surface antigen, pertussis, toxoid, diphtheria toxoid, measles virus F protein, Chlamydia trachomatis major outer membrane protein, Plasmodium falciparum circumsporozite T, P. falciparum CS antigen, Schistosoma mansoni triose phosphate isomersae, Escherichia coli TraT, and Influenza virus hemagluttinin (HA). Other suitable immunogenic carrier molecules include promiscuous T helper cell epitopes which are derived from hepatitis B virus, Bordetella pertussis, Clostridium tetani, Pertusaria trachythallina, E. coli, Chlamydia trachomatis, Diphtheria, P. falciparum, and Schistosoma mansoni (see, e.g., U.S. Pat. No. 6,906,169; U.S. Pat. Appl. Publ. No. 20030068325; Int. Appl. Publ. Nos. WO/2002/096350 and WO01/42306).
Techniques for linking a peptide to an immunogenic carrier molecule include chemical crosslinking by, e.g., formation of disulfide linkages using N-succinimidyl-3-(2-pyridyl-thio) propionate (SPDP) and succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (SMCC) (if the peptide lacks a sulfhydryl group, this can be provided by addition of a cysteine residue). These reagents create a disulfide linkage between themselves and peptide cysteine residues on one protein, and an amide linkage through the epsilon-amino on a lysine, or other free amino group in other amino acids. A variety of such disulfide/amide-forming agents are described by Jansen et al., Immun Rev 62:185-216 (1982). Other bifunctional coupling agents form a thioether rather than a disulfide linkage. Many of these thio-ether-forming agents are commercially available and include reactive esters of 6-maleimidocaproic acid, 2-bromoacetic acid, 2-iodoacetic acid, and 4-(N-maleimido-methyl)cyclohexane-1-carboxylic acid. The carboxyl groups can be activated, e.g., by combining them with succinimide or 1-hydroxyl-2-nitro-4-sulfonic acid, sodium salt.
The invention also provides polymerized forms of the above peptides, which polymerized forms have molecular weight less than 100 kDa and are capable of eliciting antibodies directed against the abnormal conformation of Aβ in amyloid plaques and/or in soluble and semi-soluble Aβ oligomers. Such polymerized forms can be produced using methods known in the art. For example, the peptide can be polymerized by a reaction with a cross linking reagent. Suitable cross-linking reagents include, but are not limited to glutaraldehyde and 1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC). Alternatively, the peptide can be polymerized by cysteine oxidation induced disulfide cross linking.
In one embodiment, polymerized forms can be produced using controlled polymerization with glutaraldehyde as described in U.S. Patent Appl. Publ. No. 2010/0284909 and Goni et al., PLoS One, 2010, 5(10): e13391. Briefly, this method involves dissolving the peptide at 3 mg/ml, in 100 mM borate buffer saline (BBS), pH 7.4; preparing fresh 1% glutaraldehyde in BBS and adding glutaraldehyde to the peptide to a final 5 mM glutaraldehyde concentration, followed by incubation in an Eppendorf block at 800 rpm at 56° C. for 16 hrs. The solution is then quenched with 0.5 M glycine to make the solution 100 mM in glycine. After five minutes the solution is diluted 1:3 with BBS, dialyzed against 2 mM BBS overnight at 4° C., aliquoted, and lyophilized. To determine the degree of aggregation, the original monomeric peptide and polymerized peptide are electrophoresed on 12.5% SDS-polyacrylamide Tris-tricine gels under reducing conditions and the peptides are detected by immunoblotting with peptide-specific antibodies. The secondary structure of the polymerized immunogens can be evaluated using circular dichroism (CD) and electron microscopy as described previously (Sadowski et al., Proc Natl Acad Sci USA, 2006, 103:18787-18792).
Pharmaceutical Compositions and Administration
The present invention also provides pharmaceutical compositions of the immunogenic modified Aβ peptides described herein. Such pharmaceutical compositions can be administered systemically. The term “systemic” as used herein includes parenteral, topical, transdermal, oral, by inhalation/pulmonary, rectal, nasal, and buccal administration. The term “parenteral” as used herein includes subcutaneous, intradermal, intravenous, intramuscular, intracranial, and intraperitoneal administration. Preferably, the peptides are administered intramuscularly, subcutaneously, orally, or intranasally in therapeutically effective amounts to treat amyloid diseases.
Mucosal immunization using the peptides of the invention can be effective to treat the encompassed pathologies (see, e.g., Goni et al., Neurosci., 2005, 133:413-421 and Goni et al., Neurosci., 2008, 153:679-686). In some embodiments, for oral immunization, the immunogen can be administered as a conjugate with cholera toxin B (CTB) subunit. Cholera toxin augments the local (gastrointestinal) and systemic serum antibody response to co-administered antigens via a Th2 cell dependent pathway (Gelinas et al., Proc Natl Acad Sci USA, 2004, 101:14657-14662).
Optimization of pharmaceutical compositions and peptide immunization protocols can be first conducted in one or more animal models of AD or other amyloid diseases. Behavioral studies can include the radial arm maze analysis, locomotor activity and spatial learning (Sigurdsson et al., J. Neurosci., 2004, 24:6277-6282 and Sadowski et al., J Neuropath Exp Neurol, 2004, 63:418-428). Amyloid and tau burden quantitation, including biochemical levels for both soluble and insoluble Aβ, as well as, Aβ oligomer levels can be performed following previously published protocols (Sadowski et al., Proc Natl Acad Sci USA, 2006, 103:18787-18792 and Scholtzova et al., J Neurosci Res, 2008, 86:2784-2791).
The invention encompasses pharmaceutical compositions comprising therapeutically effective amounts of Aβ peptides together with pharmaceutically acceptable diluents, preservatives, solubilizers, emulsifiers, adjuvants and/or carriers. Such compositions include diluents of various buffer content (e.g., Tris-HCI, acetate, phosphate), pH and ionic strength; additives such as detergents and solubilizing agents (e.g., Tween 80, Polysorbate 80), antioxidants (e.g., ascorbic acid, sodium metabisulfite), preservatives (e.g., Thimersol, benzyl alcohol) and bulking substances (e.g., lactose, mannitol); incorporation of the material into particulate preparations of polymeric compounds such as polylactic acid, polyglycolic acid, etc. or into liposomes. Hyaluronic acid may also be used, and this may have the effect of promoting sustained duration in the circulation. Such compositions may influence the physical state, stability, rate of in vivo release, and rate of in vivo clearance of the present peptides and derivatives. See, e.g., Remington's Pharmaceutical Sciences, 18th Ed. (1990, Mack Publishing Co., Easton, Pa. 18042) pages 1435-1712 which are herein incorporated by reference. The compositions may be prepared in liquid form, or may be in dried powder, such as lyophilized form. Implantable sustained release formulations are also contemplated, as are transdermal formulations.
Pharmaceutically acceptable carriers include carbohydrates such as trehalose, mannitol, xylitol, sucrose, lactose, and sorbitol. Other ingredients for use in formulations may include DPPC, DOPE, DSPC and DOPC. Natural or synthetic surfactants may be used. PEG may be used (even apart from its use in derivatizing the peptide or analog). Dextrans, such as cyclodextran, may be used. Bile salts and other related enhancers may be used. Cellulose and cellulose derivatives may be used. Amino acids may be used, such as use in a buffer formulation.
Also, the use of liposomes, microcapsules or microspheres, inclusion complexes, or other types of carriers known in the art, is contemplated.
The pharmaceutical compositions of the present invention can further comprise an adjuvant. One class of useful adjuvants includes aluminum salts, such as aluminum hydroxide, aluminum phosphate, or aluminum sulfate. Such adjuvants can be used with or without other specific immunostimulating agents such as MPL or 3-DMP, QS-21, flagellin, polymeric or monomeric amino acids such as polyglutamic acid or polylysine, or pluronic polyols. Oil-in-water emulsion formulations are also suitable adjuvants that can be used with or without other specific immunostimulating agents such as muramyl peptides (e.g., N-acetylmuramyl-L-threonyl-D-isoglutamine (thr-MDP), N-acetyl-normuramyl-L-alanyl-D-isoglutamine (nor-MDP), N-acetylmuramyl-L-alanyl-D-isoglutaminyl-L-alanine-2-(1′-2′ dipalmitoyl-sn-1-glycero-3-hydroxyphosphoryloxy)-ethyl-amine (MTP-PE), N-acetylglucsaminyl-N-acetylmuramyl-L-Al-D-isoglu-L-Ala-dipalmitoxy propylamide (DTP-DPP) TheramideTM, or other bacterial cell wall components). A suitable oil-in-water emulsion is MF59 (containing 5% Squalene, 0.5% Tween 80, and 0.5% Span 85 (optionally containing various amounts of MTP-PE) formulated into submicron particles using a microfluidizer such as Model 110Y microfluidizer (Microfluidics, Newton Mass.) as described in Int. Appl. Publ. No. WO90/14837. Other suitable oil-in-water emulsions include SAF (containing 10% Squalene, 0.4% Tween 80, 5% pluronic-blocked polymer L121, and thr-MDP, either microfluidized into a submicron emulsion or vortexed to generate a larger particle size emulsion) and RibiTM adjuvant system (RAS; containing 2% squalene, 0.2% Tween 80, and one or more bacterial cell wall components). Another class of preferred adjuvants is saponin adjuvants, such as StimulonTM (QS-21) or particles generated therefrom such as ISCOMs (immunostimulating complexes) and ISCOMATRIX. Other suitable adjuvants include incomplete or complete Freund's Adjuvant (IFA), cytokines, such as interleukins (IL-1, IL-2, and IL-12), macrophage colony stimulating factor (M-CSF), lysolecithin, tumor necrosis factor (TNF), and liposome polycation DNA particles. Such adjuvants are generally available from commercial sources.
The pharmaceutical compositions of the invention can further comprise agents which facilitate brain delivery. Non-limiting examples of such useful agents include, e.g., functionalized nanocarriers (e.g., nanoparticles coated with transferrin or transferrin receptor [TR] antibodies) and liposomes (e.g., liposomes coated with targeting molecules such as antibodies, Trojan Horses Liposomes [THL]), antibodies (e.g., antibodies against transferrin receptor [TR] and insulin receptor [HIR], BBB transmigrating Llama single domain antibodies (sdAb)), chimeric peptides (e.g., Angiopeps derived from proteins expressing the Kunitz domain), low-density lipoprotein receptor related proteins 1 and 2 (LRP-1 and 2), diphtheria toxin receptor (DTR), mesenchyme stem cells, etc.
In Vivo Treatment
Treatment in vivo, i.e., by a method where an immunogenic modified Aβ•peptide of the invention is administered to the patient, is expected to result in a reduced amyloid burden within the brain of an AD patient and has the potential to halt or slow the progression of the cognitive impairments observed in this disease. In other amyloid diseases, this treatment approach is expected to enhance clearance of the respective amyloid proteins from their target organs in a similar manner and therefore improve the condition of those patients.
Without wishing to be bound by any particular theory, it is believed that following administration of the immunogenic modified Aβ peptides of the invention, the induced antibodies will bind to Aβ. The Aβ is preferably, although not necessarily, soluble Aβ and is free, e.g, not irreversibly bound to an amyloid plaque or other component. Free Aβ includes, but is not limited to, circulating Aβ in blood; free Aβ in the interstitial fluid in the brain; and Aβ bound to a ligand such as a naturally occurring plasma protein, e.g., albumin or transthyretin. Normally, equilibrium is presumed to exist between free Aβ in circulation and Aβ within the brain or other affected organs. A reduction in free Aβ in the circulation by binding to Aβ antibodies induced by the immunogenic modified Aβ peptides of the present invention (which antibodies do not cross the blood-brain-barrier (BBB) or have a saturated uptake) can therefore result in an efflux of Aβ out of the brain or other affected organs to re-establish the equilibrium.
In one embodiment, the patient is treated in a manner so as to increase the selective permeability of the blood-brain barrier (BBB), allowing the transport of Aβ antibodies into the brain from the blood. Aβ bound to Aβ-binding antibodies may be shuttled out of the brain or may be degraded within the central nervous system. The net effect will be a reduced concentration of Aβ within the interstitial fluid. Treatments to selectively increase the permeability of the BBB in a patient include, but are not limited to, the administration of about 1 to about 1000 μg/kg body weight, preferably about 10 to about 100 μg/kg bodyweight, of IGF-I (e.g., as a bolus injection to a patient about 0.5 to 10 hours, preferably about 1 hour, before the Aβ peptide administration). Also, even without selective permeabilization with a drug such as IGF-I, the BBB may be compromised in AD so that Aβ-binding antibodies that normally do not enter the brain, or have a saturated uptake, may access the brain more readily. Hence, Aβ clearance mediated by these antibodies may be partially from within the brain.
The specific dosage regimen and amounts administered for the peptides of the present invention will be determined by the attending physician, considering various factors which modify the action of drugs, e.g. the age, condition, body weight, sex and diet of the patient, the disease state, other medications administered, and other clinical factors. An effective amount to treat the disease would broadly range (e.g., between about 0.1 mg and about 20 mg per kg body weight of the recipient per day), and may be administered as a single dose or divided doses. If needed, the treatments can be continued throughout the life of the patient.
Subjects amenable to treatment in accordance with the methods of the present invention include individuals at risk of developing an amyloid related disease but not showing symptoms, as well as subjects presently showing symptoms. Non-limiting examples of diseases subject to treatment include, e.g., Alzheimer's disease (AD), Lewy Body Dementia, Frontotemporal dementia, type 2 diabetes, Huntington's disease, Parkinson's disease, amyloidosis associated with hemodialysis for renal failure, Down syndrome, hereditary cerebral hemorrhage with amyloidosis, kuru, Creutzfeldt-Jakob disease, Gerstmann-Straussler-Scheinker disease, fatal familial insomnia, British familial dementia, Danish familial dementia, familial corneal amyloidosis, Familial corneal dystrophies, medullary thyroid carcinoma, insulinoma, isolated atrial amyloidosis, pituitary amyloidosis, aortic amyloidosis, plasma cell disorders, familial amyloidosis, senile cardiac amyloidosis, inflammation-associated amyloidosis, familial Mediterranean fever, systemic amyloidosis, familial systemic amyloidosis, chronic traumatic encephalopathy, progressive supranuclear palsy, and corticobasal degeneration.
The present methods and compositions are suitable for prophylactic treatment of individuals who have a known genetic risk of AD or another encompassed condition. Genetic markers associated with a risk of AD include, among others, mutations in the APP gene, particularly mutations at position 717 and positions 670 and 671 referred to as the Hardy and Swedish mutations, respectively. Other markers of AD risk include, e.g., mutations in the presenilin genes, PS1 and PS2, and ApoE4, family history of AD, hypercholesterolemia or atherosclerosis.
Treatment can be monitored and adjusted by assaying antibody, or activated T-cell or B-cell responses to the pathological peptides/proteins. Typically, repeated dosages are given if the immune response starts to fade.
The present invention is described below in working examples which are intended to further describe the invention without limiting the scope thereof.
Example 1
Alzheimer's disease (AD) is the most common of the conformational neurodegenerative disorders. Current treatments for AD are largely symptomatic and minimally effective. Major problems with previous immunotherapeutic approaches include: potential of toxicity from autoimmune encephalitis, tau-related pathology not being addressed, and the need to effectively clear congophilic angiopathy (CAA).
The Aβ 1-42 peptide (DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA; SEQ ID NO: 9) and portions or variations of the same have been extensively used as immunogens for therapy of AD related pathology in animal models and in a few clinical trials. The results varied but only addressed the Aβ immunodominant epitopes, without targeting the toxic effect of oligomers, or reducing congophilic angiopathy (CAA).
Short Aβ sequences encompassing only 12 to 16 of the amino terminal residues proved to have some beneficial effects as immunogens for the therapy of amyloid related AD. However, these short sequence peptides do not have the proper configuration as found in pathologically oligomerized or fibrillized Aβ species. Use of these short peptides only allows detection of the amino terminus in a conformation not found in pathological Aβ species.
As disclosed herein, the present inventors have developed a novel immunomodulatory therapeutic approach that uses modified non-toxic non-fibrillogenic Aβ peptide Aβ 1-30 K18K19 (Aβ1-30KK; DAEFRHDSGYEVHHQKLKKFAEDVGSNKGA; SEQ ID NO: 1) to overcome these limitations of vaccination. The K18K19 mutation is believed to give flexibility to the Aβ peptide structure and produce an immunogen with a cryptic epitope at the amino terminus. Such immunogen induces an immune response which is more targeted to toxic oligomeric and fibrillar Aβ species. As shown herein, immunization with the peptide Aβ 1-30 K18K19 can induce behavioral benefits in mice models of AD. Such behavioral benefits are associated with reductions in Aβ oligomers and an immune response that recognized aggregated Aβ. This approach has been tested herein in mice with both amyloid plaques and tangle pathology (3×Tg mice; Oddo et al Neuron 2003; 39:409-21) and mice with extensive CAA related pathology/vascular amyloid deposits (TgSwDI; Davis et al JBC 2004; 279:20296-306).
Without wishing to be bound by any particular theory, it is hypothesized herein that the antibodies produced against Aβ 1-30 K18K19 and other immunogenic peptides of the invention would not only bind the Aβ peptides in a novel manner but will also alter their structure rendering the Aβ peptides non fibrillogenic/amyloidogenic, reversing their toxicity. The disappearance of the immunodominant epitope is expected to lower the risk of inducing self-toxicity and exacerbation of CAA.
Materials and Methods
Peptide.
Aβ 1-30 K18K19 (DAEFRHDSGYEVHHQKLKKFAEDVGSNKGA; SEQ ID NO: 1) was synthesized on an ABI 430A peptide synthesizer (AME Bioscience, Chicago, Ill.) at the Keck peptide synthesis facility at Yale University, CT, using a Vydac C18 preparative column, 2.5×30 cm (Vydac Separations, Hesperia, Calif.). Standard protocols for tBOC (tert-butyloxycarbonyl) chemistry were used. The peptide was subsequently cleaved from the resins using hydrofluoric acid and purified by high-pressure liquid chromatography (HPLC) on a Vydac C18 preparative column using linear gradients from 0-70% of acetonitrile in 0.1% trifluoroacetic acid. Mass spectroscopy of the lyophilized end-product was used to verify the expected molecular weight.
From the original sequence of the Aβ peptide only the first 30 amino acids were used to avoid fibrillogenic sequences at the carboyl-terminus; and two hydrophobic residues were mutated at positions 18 and 19 replacing Phenylalanine and Valine by two Lysine residues with epsilon amino groups that specifically give a particular secondary structure. These positively charged amino acids interfere with the expression of an immunodominant epitope and give flexibility to the middle structure of the molecule; hence, making available an otherwise cryptic epitope at the amino terminus of the peptide.
Animals.
3×Tg mice (Oddo et al Neuron 2003; 39:409-21) and TgSwDI mice (Davis et al JBC 2004; 279:20296-306) were used in the experiments. The animals were maintained on a 12-hour light-dark cycle, and had access to food and water ad libitum. The animal care was in accordance with institutional guidelines.
Immunization.
Starting at the age of 3 months, mice were immunized 4 times biweekly, subcutaneously with 50 μg peptide/animal in sterile saline:Alum (9:1), and thereafter 4 times bimonthly with 25 μg peptide/animal until the age of 12 months. At the age of 15-16 months the mice were subject to locomotor and cognitive behavioral testing (radial arm maze), followed by histological and biochemical analysis.
Antibody Levels.
IgM and IgG antibody titers cross-reactive with Aβ1-40 and Aβ1-42 were determined at T0, T1 (after the 6th inoculation), and Tf (at the time of sacrifice) using an enzyme-linked immunosorbent assay (ELISA) as described previously (Jimenez-Huete et al., Alzheimers Reports 1998; 1:4147) in which Aβ or its derivative is coated onto microliter wells. The antibodies were detected by a goat anti-mouse IgG linked to a horseradish peroxidase (Amersham Pharmacia Biotech, Piscataway, N.J.) or a goat anti-mouse IgM peroxidase conjugate (Sigma, A8786), and tetramethyl benzidine (Pierce, Rockford, Ill.) was the substrate.
Radial Arm Maze Test (Behavioral Analysis).
Animals were kept in test room throughout the experiment, behind a cover to prevent view of the apparatus and room. Each animal underwent 2 days of adaptation, consisting of 15 minutes of maze exploration (2 subjects at a time), with 3 pieces of fruit loops in each arm. Subjects were exposed to arm doors only on day 2. Animals were food deprived 1 day before the first adaptation session and maintained at approximately ten percent body weight loss. Fruit loops were added to normal diet 5 days before deprivation schedule started. Animals entered and exited the apparatus through the center of the maze. Testing included recording correct and incorrect arms entered. Each trial was initiated by placing the mouse in the center of the maze and all doors into the arms were subsequently opened. After entry into an arm, the animal had to find and eat the reinforcer before the door was reopened to allow the animal to re-enter the center of the maze. Testing ended when all eight arms had been entered and reinforcers eaten. Re-entry into an arm constituted an error. Total number of errors and time to enter all eight arms were recorded. The animals were allowed access to food for up to 3-4 hours daily, depending on their body weight loss. The corners and holes in the maze were cleaned with 95% ethanol after each animal and the arms.
Histology.
Mice were sacrificed at 15-16 months of age after behavioral testing and their brains were processed for histology with subsequent stereological analysis. Mice were anesthetized with sodium pentobarbital (150 mg/kg, intraperitoneally), perfused transaortically with phosphate buffer and the brains processed as previously described (Sigurdsson et al., Neurobiol. Aging 1996; 17:893-901). The right hemisphere was immersion fixed in periodate-lysine-paraformaldehyde (PLP), whereas the left hemisphere was snap-frozen for measurements of Aβ levels using established ELISA methods (Mehta et al., Arch. Neurol. 2000; 57:100-105). Serial coronal sections (40 μm) were cut and every fifth section was stained with 6E10 which recognizes Aβ and stains both pre-amyloid and Aβ plaques (Kim et al., Neurosci Res Comm 1990; 7:113-122). After sectioning, the series were placed in ethylene glycol cryoprotectant and stored at −20° C. until used. Staining was performed as previously described (Sigurdsson et al., Neurobiol. Aging 1996; 17:893-901; Soto et al., Nat Med 1998; 4:822-826). Briefly, sections were incubated in 6E10 (provided by Richard Kascsak, Institute for Basic Research) primary antibody that selectively binds to human Aβ at a 1:1000 dilution. A mouse-on-mouse immunodetection kit (Vector Laboratories, Burlingame, Calif.) was used in which the anti-mouse IgG secondary antibody was used at a 1:2000 dilution. The sections were reacted in 3,3-diaminobenzidine tetrahydrochloride (DAB) with nickel ammonium sulfate (Ni; Mallinckrodt, Paris, Ky.) intensification.
Image Analysis.
Immunohistochemistry of tissue sections was quantified with a Bioquant image analysis system, and unbiased sampling was used (West et al., Trends Neurosci. 1999; 22:51-61). All procedures were performed by an individual blind to the experimental condition of the study. Cortical area analyzed was dorsomedially from the cingulate cortex and extended ventrolaterally to the rhinal fissure within the right hemisphere. The area of the grid was 800×800 μm2 and amyloid load was measured in 20 frames per mouse (each: 640×480 μm2), chosen randomly. The Aβ burden is defined as the percentage of area in the measurement field occupied by reaction product.
Results
Starting at the age of 3 months, 3×Tg and TgSwDI mice were immunized with Aβ 1-30 K18K19 (Aβ1-30KK; DAEFRHDSGYEVHHQKLKKFAEDVGSNKGA; SEQ ID NO: 1) peptide, Aβ 1-42 peptide (DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA; SEQ ID NO: 9), vehicle control, polymerized British amyloidosis related peptides (pABri) (monomer sequence CSRTVKKNIIEEN; SEQ ID NO: 8), or a combination of Aβ 1-30 K18K19 and pABri, until the age of 12 months. At the age of 15-16 months the mice were subject to locomotor and cognitive behavioral testing (radial arm maze), followed by histological and biochemical analysis.
Locomotor testing showed no significant differences between all tested groups in both mice models.
As shown in FIG. 1, a strong IgG immune response to all Aβ forms was observed when the test animals were immunized with modified Aβ peptide Aβ 1-30 K18K19. This effect was accompanied by a cognitive preservation (FIG. 2) and a significant reduction of cortical and hippocampal amyloid burden (FIG. 3) in both animal models.
Taken together, as demonstrated herein, immunization with modified Aβ peptide Aβ 1-30 K18K19 results in a statistically significant cognitive benefit and pathology burden reduction which makes this peptide an excellent candidate for immunotherapy of AD and other amyloid disorders.
Example 2
3×Tg mice have both amyloid and Tau pathology (Oddo et al., Neurobiol. Aging, 24(8):1063-1070, 2003). This mouse model most closely resembles pathology in human Alzheimer's disease. Antibody PHF-1 detects Tau phosphorylated at Ser396/Ser404 (hyperphosphorylated Tau) (Bhaskar et al., Neuron, 68(1):19-31, 2010). PHF-1 immunoreactivity reflects Tau-related pathology.
FIGS. 4A-B show the PHF-1 immunohistochemical quantitation in the cortex (A) and CA1 hippocampus (B) of 3×Tg mice immunized with Aβ 1-30 K18K19 peptide (Aβ1-30KK) (SEQ ID NO: 1), vehicle control, polymerized British amyloidosis related peptides (pABri) (monomer sequence SEQ ID NO: 8), or a combination of Aβ1-30KK and pABri. Tg-pABri and Tg combined treated mice show clear reductions in Tau pathology compared to controls, while Tg-Aβ1-30KK treated mice do not show Tau pathology reductions. FIG. 4C shows the amyloid burden in the hippocampus of control Tg, Tg pABri, TgAβ1-30KK and combination treated mice. All treated mice demonstrate a clear reduction in the amyloid burden compared to controls.
FIG. 5 shows an immunoblot developed with antibody PHF-1 in soluble brain homogenates. All treatments including Aβ 1-30 K18K19 peptide (Aβ1-30KK) (SEQ ID NO: 1) alone and the combination of Aβ1-30KK with pABri showed a marked reduction in the band at 65 kDa when compared to the Group 3 (controls inoculated with vehicle), with the most reduction seen in the combined treated group. The same result was repeated in all the animals of each group (n=8). The reduction in the band at 65 kDa reflects reductions in oligomers which are the toxic soluble form of phospho Tau (Yang et al., Journal of Alzheimer's Disease, 24(2):269-285, 2011; Scholtzova et al., Journal of Neuroscience, 29:1846-1854, 2009). The data show that although Aβ1-30KK has no sequence homology with Tau, it can influence the toxic oligomeric forms of Tau.
Comparison of FIGS. 4 and 5 confirms that Aβ1-30KK does not affect the precipitated tau but affects the soluble oligomeric form of pTau because it has a similar secondary structure to the one generated in the Aβ1-30KK.
FIG. 6 shows the reduction, compared to controls, in soluble Aβ1-40 (SEQ ID NO: 10) and Aβ1-42 (SEQ ID NO: 9) in brain homogenates from animals treated with Aβ 1-30 K18K19 peptide (Aβ1-30KK) (SEQ ID NO: 1), pABri (monomer sequence SEQ ID NO: 8) or combined Aβ1-30KK with pABri. In case of pABri, there is a proportional greater reduction in the soluble Aβ1-42 levels compared to soluble Aβ1-40 levels, demonstrating that the therapy acts more on the toxic oligomeric β-sheet structure of Aβ1-42 than on the less oligomeric Aβ1-40.
The present invention is not to be limited in scope by the specific embodiments described herein. Indeed, various modifications of the invention in addition to those described herein will become apparent to those skilled in the art from the foregoing description and the accompanying figures. Such modifications are intended to fall within the scope of the appended claims. It is further to be understood that all values are approximate, and are provided for description.
Patents, patent applications, publications, product descriptions, and protocols are cited throughout this application, the disclosures of which are incorporated herein by reference in their entireties for all purposes.

## Claims

1. A polymerized form of an immunogenic modified Aβ peptide, which peptide contains Lysine substitutions in amino acid positions corresponding to amino acids 18 and 19 of Aβ1-42, which polymerized form is covalently bonded to an adjuvant polypeptide when produced using controlled polymerization with glutaraldehyde, and is soluble and non-fibrillogenic.

2. The polymerized form of claim 1, wherein said modified Aβ peptide is selected from the group consisting of Aβ 1-30 K18K19 (DAEFRHDSGYEVHHQKLKKFAEDVGSNKGA; SEQ ID NO: 1), Aβ 1-40 K18K19 (DAEFRHDSGYEVHHQKLKKFAEDVGSNKGAIIGLMVGGVV; SEQ ID NO: 2), Aβ 1-42 K18K19 (DAEFRHDSGYEVHHQKLKKFAEDVGSNKGAIIGLMVGGVVIA; SEQ ID NO: 3), Aβ 1-20 K18K19 (DAEFRHDSGYEVHHQKLKKF; SEQ ID NO: 4), Aβ 10-30 K18K19 (YEVHHQKLKKFAEDVGSNKGA; SEQ ID NO: 5), Aβ 10-40 K18K19 (YEVHHQKLKKFAEDVGSNKGAIIGLMVGGVV; SEQ ID NO: 6), and Aβ 10-42 K18K19 (YEVHHQKLKKFAEDVGSNKGAIIGLMVGGVVIA; SEQ ID NO: 7).

3. The polymerized form of claim 1, wherein the adjuvant polypeptide is an enterotoxin, tetanus toxoid, or flagellin.

4. The polymerized form of claim 3, wherein the enterotoxin is Cholera toxin B (CTB) subunit.

5. A pharmaceutical composition comprising one or more of the polymerized forms of claim 1 and a pharmaceutically acceptable carrier or diluent.

6. The pharmaceutical composition of claim 5 formulated for mucosal administration.

7. The pharmaceutical composition of claim 5 formulated for oral administration.

8. A method for treating a patient suffering from an amyloid disease or a tauopathy comprising administering to the patient a therapeutically effective amount of the polymerized form of claim 1.

9. The method of claim 8, wherein two or more different polymerized forms are administered.

10. The method of claim 8, further comprising administering a polymerized British amyloidosis related peptide (pBri).

11. The method of claim 10, wherein the monomer of the pBri peptide has the sequence CSRTVKKNIIEEN (SEQ ID NO: 8).

12. The method of claim 8, wherein the amyloid disease is selected from the group consisting of Alzheimer's disease, Lewy Body Dementia, Frontotemporal dementia, Parkinson's disease, Down syndrome, Creutzfeldt-Jakob disease, Gerstmann-Straussler-Scheinker disease, chronic traumatic encephalopathy, and progressive supranuclear palsy.

13. The method of claim 8, wherein the amyloid disease is associated with accumulation of Aβ in one or more bodily fluids.

14. The method of claim 8, wherein the polymerized form is administered mucosally.

15. The method of claim 8, wherein the polymerized form is administered orally.

16. The method of claim 8, wherein the patient is human.