# Patent

## Title
Pharmaceutical composition for treating liver diseases

## Abstract

The present invention relates to a pharmaceutical composition for treating liver diseases, comprising a miRNA mimic containing a single strand RNA molecule of hsa-miR-21-3p (SEQ ID No: 35). The miRNA mimic of the present invention can be used to treat liver diseases through regulating the expression of methionine adenosyltransferase 2A and 2B (MAT2A and MAT2B), acetyl-CoA carboxylase 1 and 2 (ACACA and ACACB), diglyceride acyltransferase 2 (DGAT2), and so on. In addition, the present invention also relates to a method for reducing the expression of the above-mentioned enzymes.

## Background
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a Divisional of U.S. application Ser. No. 14/496,280, filed on Sep. 25, 2014, which claims priority under 35 U.S.C. §119(a) on Patent Application No. 103104537, filed in Taiwan on Feb. 12, 2014, the contents of each are hereby expressly incorporated by reference into the present application.


BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates to a pharmaceutical composition for treating liver diseases, comprising a miRNA mimic containing a single strand RNA molecule of hsa-miR-21-3p (SEQ ID No: 35). In addition, the present invention also relates to a method for treating liver diseases and reducing the expression of specific enzymes.
2. Description of the Related Art
Nonalchoholic fatty liver disease (NAFLD) is rapidly becoming one of the most common liver disease because of growing prevalence of overweight and obesity. Generally, NAFLD is defined by fat accumulation, mainly triglycerides, in hepatocytes exceeding 5% of its weight. In the progress of NAFLD, intrahepatic lipid accumulation and growth of lipid droplets result in different degrees of inflammation, thereby resulting in liver fibrosis. As the clinical pathologic spectrum, liver fibrosis may progress advanced cirrhosis, hepatocellular carcinoma, hepatic decompensation, and have increased all-cause mortality. However, there is no standard drug treatment or specific therapy to reverse fatty liver disease. Nowadays, researchers are going to uncover what processes may trigger fat build-up in the liver and how to prevent and treat the fatty liver disease.
Methionine adenosyltransferase (MAT) is the cellular enzyme that catalyzes the synthesis of S-adenosyl methionine (SAM), the principal biological methyl donor and a key regulator of hepatocyte proliferation, death and differentiation[1,2]. Two genes, MAT1A and MAT2A, encode 2 distinct catalytic MAT isoforms. A third gene, MAT2B, encodes a MAT2A regulatory subunit. MAT1A is specifically expressed in the adult liver, whereas MAT2A is widely distributed[3-5]. Because MAT isoforms differ in catalytic kinetics and regulatory properties, MAT1A-expressing cells have considerably higher SAM levels than do MAT2A-expressing cells[6,7]. In hepatocellular carcinoma (HCC), the down-regulation of MAT1A and the up-regulation of MAT2A occur, which is known as the MAT1A:MAT2A switch[8-11]. The switch accompanied with up-regulation of MAT2B results in lower SAM contents, which provide a growth advantage to hepatoma cells[2,4,6,12,13]. SAM can selectively induce pro-apoptotic Bcl-Xs in hepatoma cells, but not in normal hepatocytes, through alternative splicing[14]. In addition, increased MAT2B expression in HCC also results in decreased SAM levels and facilitates cancer cell growth[15]. Because MAT2A and MAT2B play crucial role in facilitating the growth of hepatoma cells, they are valid targets for antineoplastic therapy. Recent studies have shown that silencing MAT2A and MAT2B by using small interfering RNA substantially suppress growth and induce apoptosis in hepatoma cells[16-19].
Acetyl-CoA-carboxylase, which catalyses the carboxylation of acetyl-CoA to form malonyl-CoA, exists in 2 isoforms (alpha and beta) that are separately encoded by ACACA and ACACB in mammals. ACACA, a cytosolic enzyme, is the first committed step of fatty acid synthesis in lipogenic tissue[19]. Carnitine-palmitoyl-CoA transferase I (CPT1), a rate-limiting enzyme that shuttles long-chain fatty acyl-CoAs into the mitochondria for oxidation, is rapidly inhibited by the ACACB-produced malonyl-CoA[20, 21]. Diglyceride acyltransferase (DGAT), the terminal and the only committed enzyme in the biosynthesis of triacylglycerol, plays a key role in hepatic lipid droplet accumulation[22, 23]. There are 2 forms of diglyceride acyltransferase which are separately encoded by DGAT1 and DGAT2. Recent studies have shown that fatty liver disease can be ameliorated or reversed by reducing the expression of ACACA, ACACB, or DGAT2, indicating that pharmacologically inhibiting these genes could be a suitable approach to treating of NAFLD[24, 25].
Berberine is an isoquinoline alkaloid isolated from various medicinal herbs such as Coptis chinensis, and it has a wide range of pharmacological effects including anti-cancer, anti-microbial, anti-inflammatory, and anti-diabetic effects[28-29]. Recent studies have focused on its anti-tumor effects, including anti-proliferation, anti-invasion and apoptosis induction in broad tumor cell types[29-38]. In HCC, berberine has been reported to inhibit cell growth and survival through cell cycle arrest and the activation of autophagic and mitochondrial apoptotic cell death[39-41]. In addition, some reports have shown that berberine has hypoglycemic, hypolipidemic and LDL-lowing effects, and animal studies have proved that berberine reduces the liver fat content in vivo.
MicroRNAs (miRNAs) are small non-coding RNA molecules composed of 21-23 nucleotides that play a critical role in a wide variety of biological processes, including development, proliferation, and death[42, 43]. The deregulated expression of miRNAs is observed in numerous human cancer types, and they can act as tumor suppressors or oncogenes in the tumorigenic process[44, 45]. Mature miRNAs typically direct their posttranscriptional repression by pairing the seed region of the miRNA to 3′ UTRs, the non-coding sequence at the 3′ end of target genes, leading to mRNA destabilization and translational silencing[46,47]. The seed region of miRNAs locates at the 5′ end, from the second to eighth nucleotide. When the seed region pairs with the 3′ UTR of the target gene, it silences the gene. It is not necessary for the miRNA being completely complementary to the 3′ UTR of the target gene. The processing of the precursor miRNA (pre-miRNAs) hairpin generates an miRNA duplex consisting of a guide strand and a passenger strand (also termed as miRNA and miRNA*). By convention, a guide strand is selectively loaded onto an Argonaute (AGO) protein to form an miRNA-induced silencing complex (miRISC), and the passenger strand is believed to be preferentially degraded because of its lower steady-state level[48]. However, current research shows that numerous miRNA* species accumulate to substantial levels, and endogenous miRNA genes do not universally exclude miRNA* species from functional miRISC complexes, which suggests that miRNA* species should be considered[49-54].
SUMMARY OF THE INVENTION
In this invention, we used miRNA microarray and found that the expression level of hsa-miR-21-3p increased after berberine treatment in human hepatoma cell line (HepG2) and primary human hepatocytes (PHHs). With whole-genome microarray, bioinformatics software and a series of experiments, we have found that the miR-21-3p (one of the mature miRNAs of MIR21 gene) mimic reduces the expression of MAT2A, MAT2B and EEF2K in hepatocellular carcinoma cells, increases intracellular SAM concentration, inhibits cancer cell growth and induces apoptosis in hepatoma. In addition, the miR-21-3p mimic also decrease the lipid droplet contents in primary human hepatocytes, inhibits the expression of ACACA, ACACB and DGAT2, and reduces the expression of lipid biosynthesis-related genes, including HLCS, MTOR, RPTOR, in primary human hepatocytes, thereby decreasing lipid biosynthesis in liver, promoting lipid oxidation and ameliorates fatty liver diseases.
One object of the present invention is to provide a pharmaceutical composition comprising a microRNA mimic which inhibits cancer cell growth and induces apoptosis in hepatoma, decreases lipid biosynthesis, promotes lipid oxidation and ameliorates fatty liver diseases.
Another object of the present invention is to provide a method for decreasing the expression of methionine adenosyltransferase 2A and 2B (MAT2A and MAT2B), in which these enzymes relate to inhibition of cancer cell growth and induction of apoptosis in hepatoma, and can be applied for the treatment for liver cancers.
Yet another object of the present invention is to provide a method for decreasing the expression of acetyl-CoA carboxylase 1 and 2 (ACACA and ACACB) and diglyceride acyltransferase 2 (DGAT2), in which these enzymes relate to reduction of lipid metabolism and triglyceride biosynthesis, and can be applied for the treatment of fatty liver diseases.
The present invention provides a pharmaceutical composition for treating liver diseases, comprising a microRNA mimic containing a single strand RNA molecule of hsa-miR-21-3p (SEQ ID No: 35).
In the preferred embodiments of the present invention, said pharmaceutical composition comprise a microRNA mimic which is a single strand RNA molecule of hsa-miR-21-3p (SEQ ID No: 35).
In the preferred embodiments of the present invention, said microRNA mimic is chemically modified for not being degraded by RNase; more preferably, said microRNA mimic is chemically modified to form a O2′, C4′-methylene bridge between the second O molecule and fourth C molecule of riboses, which is a stable locked nucleic acid (LNA) form. Such a chemically modified nucleic acid molecule can be directly administered to patients without any drug carrier.
In the preferred embodiments of the present invention, said microRNA mimic is delivered into target cells by a general delivery method used for RNA drugs, including delivering said microRNA mimic into target cells by transfection and/or conjugate delivery. Regarding with transfection, said microRNA mimic is delivered into target cells by using liposome, exosome, nanoparticle, virus, and the like; in which said nanoparticle comprises lipid nanoparticle (LNP) or polymer nanoparticle such like chitosan. Regarding with conjugate delivery, said microRNA mimic is delivered into target cells after binding with an aptamer or cholesterol. For example, the microRNA mimic of the present invention is directly modified to bind with an aptamer; therefore, when it is administered into the body, the aptamer will bind the target cells and delivers the microRNA mimic of the present invention into the target cells. Alternatively, the microRNA mimic of the present invention is covalently binding with cholesterol; therefore, when the conjugate is administered into the body, it will be taken by hepatic cells having low-density lipoprotein (LDL) receptors on their surfaces. Moreover, the combination of conjugate delivery and transfection also can be used. For example, the microRNA mimic of the present invention can be conjugated with an aptamer to form a conjugate, and then the conjugate is transfected into the target cells by a transfecting agent (such like liposome).
In the preferred embodiments of the present invention, said pharmaceutical composition can be administered orally or parenterally; more preferably, it is administered by injection; even more preferably, it is administered by intravenous injection.
In the preferred embodiments of the present invention, said liver diseases comprise liver cancers and fatty liver diseases.
In the preferred embodiments of the present invention, said pharmaceutical composition treats liver cancers though decreasing the expression of methionine adenosyltransferase 2A and 2B (MAT2A and MAT2B).
In the preferred embodiments of the present invention, said pharmaceutical composition treats fatty liver diseases though decreasing the expression of acetyl-CoA carboxylase 1 and 2 (ACACA and ACACB) and diglyceride acyltransferase 2 (DGAT2).
The present invention also provides a use of a microRNA mimic containing a single strand RNA molecule of hsa-miR-21-3p (SEQ ID No: 35) for preparing a drug for liver cancers.
The present invention also provides a use of a microRNA mimic containing a single strand RNA molecule of hsa-miR-21-3p (SEQ ID No: 35) for preparing a drug for fatty liver diseases.
The present invention also provides a method for decreasing the expression of acetyl-CoA carboxylase 1 and 2 (ACACA and ACACB) and diglyceride acyltransferase 2 (DGAT2) by administering berberine or a microRNA mimic containing a single strand RNA molecule of hsa-miR-21-3p (SEQ ID No: 35).
In the preferred embodiments of the present invention, the method for decreasing the expression of ACACA, ACACB and DGAT2 comprises administering berberine or a microRNA mimic which is a single strand RNA molecule of hsa-miR-21-3p (SEQ ID No: 35).
Yet, the present invention provides a method for decreasing the expression of methionine adenosyltransferase 2A and 2B (MAT2A and MAT2B) by administering berberine or a microRNA mimic containing a single strand RNA molecule of hsa-miR-21-3p (SEQ ID No: 35).
In the preferred embodiments of the present invention, the method for decreasing the expression of MAT2A and MAT2B comprises administering berberine or a microRNA mimic which is a single strand RNA molecule of hsa-miR-21-3p (SEQ ID No: 35).
The present invention provides a pharmaceutical composition comprising a microRNA mimic which is a single strand RNA molecule of hsa-miR-21-3p (SEQ ID No: 35), wherein the microRNA mimic of hsa-miR-21-3p improves liver function through many working mechanisms and induces cancer cell apoptosis. Thus, it is potential for treating liver diseases.



BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1A represents the scatter plot showing the comparison of the miRNA expression profiles between berberine-treated and untreated HepG2 cells. The arrow indicates that hsa-miR-21-3p is the only miRNA that increases in HepG2 cell line after 40 μM berberine treatment for 2 h and 4 h. The diagonal lines represent that the induced fold changes are between the boundary values of 2 and 0.5.
FIG. 1B represents the changes of HepG2 cells stimulated by the time course of 40 μM berberine treatment for up to 8 h compared with the untreated control. The hsa-miR-21-3p and hsa-miR-21-5p levels are measured by qRT-PCR, which are shown in folds. The data are represented as the mean±standard deviation for 3 independent experiments. The asterisk mark “*” indicates a significant difference with P<0.05.
FIG. 2 represents the comparison of the MIR21 gene sequences of 18 mammalian species (SEQ ID No: 45 to SEQ ID No: 62), which shows that the MIR21 gene is conserved over the mammalian evolution. The asterisk mark indicates the seed region of miRNAs.
FIG. 3A represents that the target genes of miR-21-3p predicted using the miRanda algorithm and an mirSVR score threshold of <−0.1 are overlapped with the genes negatively regulated after berberine treatment in HepG2 cells according to the microarray data, in which 5 genes targets are present in both groups: MAT2A, DIDO1, EEF2K, NBPF8 and TMEM137.
FIG. 3B represents the mRNA expression levels of MAT2A, DIDO1, EEF2K, NBPF8, and TMEM137 in HepG2 cells after transfection of 50 nM of miR-21-3p mimic or negative-control (NC) mimic. The mRNA expression is measured using qRT-PCR. The data are represented as the mean±standard deviation for 3 independent experiments. The asterisk mark “*” indicates a significant difference with P<0.05.
FIG. 3C represents the mRNA expression levels of HepG2 cells transfected with 100 nM of miR-21-3p hairpin inhibitor or negative-control (NC) inhibitor for 24 h, and then stimulated by the time course of 40 μM berberine treatment for 0-8 h. The mRNA expression is measured using qRT-PCR. The data are represented as the mean±standard deviation for 3 independent experiments. The asterisk mark “*” indicates a significant difference with P<0.05.
FIG. 4A represents the mirSVR score of each seed complementary site in the 3′ UTRs of MAT1A, MAT2A and MAT2B.
FIG. 4B represents the mRNA expression levels of MAT1A, MAT2A and MAT2B in HepG2 cells transfected with 50 nM of miR-21-3p mimic or negative-control (NC) mimic for 24 h and 48 h. The mRNA expression is measured using qRT-PCR. The data are represented as the mean±standard deviation for 3 independent experiments. The asterisk mark “*” indicates a significant difference with P<0.05.
FIG. 4C represents the protein expression levels of MAT1A, MAT2A and MAT2B in HepG2 cells transfected with 50 nM of miR-21-3p mimic or negative-control mimic for 72 h. The protein expression is measured using Western blot analysis. GAPDH was used as the loading control.
FIG. 4D represents the mRNA expression levels of MAT1A, MAT2A and MAT2B in HepG2 cells transfected with 100 nM of miR-21-3p inhibitor or negative-control (NC) inhibitor for 24 h and 48 h. The mRNA expression is measured using qRT-PCR. The data are represented as the mean±standard deviation for 3 independent experiments. The asterisk mark “*” indicates a significant difference with P<0.05.
FIG. 4E represents the mRNA expression levels of MAT1A, MAT2A and MAT2B in HepG2 cells stimulated by the time course of 40 μM berberine treatment for up to 8 h compared with the untreated control. The mRNA expression is measured using qRT-PCR. The asterisk mark “*” indicates a significant difference with P<0.05.
FIG. 4F represents the intracellular SAM concentration of the HepG2 cells transfected with 50 nM of miR-21-3p mimic or negative-control (NC) mimic for 72 h, which is shown in folds. The data are represented as the mean±standard deviation for 3 independent experiments. The asterisk mark “*” indicates a significant difference with P<0.05.
FIG. 5A is a schematic representation showing the putative 3′ UTR binding sites of MAT1A (SEQ ID No: 63 to SEQ ID No: 66) for hsa-miR-21-3p.
FIG. 5B is a schematic representation showing the putative 3′ UTR binding sites of MAT2B (SEQ ID No: 67 to SEQ ID No: 68) for hsa-miR-21-3p.
FIG. 5C is a schematic representation showing the mutated seed binding sites in 3′ UTR of MAT2A, which inserted downstream of the luciferase of the pMIR-reporter vector. It also shows the luciferase activity of HEK293T and HepG2 cells tri-transfected with either wild-type or mutant pMIR-reporter vector, the Renilla luciferase reporter plasmid pRL-TK, and either hsa-miR-21-3p mimic or negative-control mimic. The data are represented as the mean±standard deviation for 3 independent experiments. The asterisk mark “*” indicates a significant difference with P<0.05.
FIG. 5D is a schematic representation showing the mutated seed binding sites in 3′ UTR of MAT2B, which inserted downstream of the luciferase of the pMIR-reporter vector. It also shows the luciferase activity of HEK293T and HepG2 cells tri-transfected with either wild-type or mutant pMIR-reporter vector, the Renilla luciferase reporter plasmid pRL-TK, and either hsa-miR-21-3p mimic or negative-control mimic. The data are represented as the mean±standard deviation for 3 independent experiments. The asterisk mark “*” indicates a significant difference with P<0.05.
FIG. 6A represents the number of viable HepG2 cells transfected with 50 nM of miR-21-3p mimic or negative-control (NC) mimic for 48 h, which is quantified using trypan blue dye exclusion assay. The data are represented as the mean±standard deviation for 3 independent experiments. The asterisk mark “*” indicates a significant difference with P<0.05.
FIG. 6B represents the cellular proliferation (shown in folds) of HepG2 cells transfected with 50 nM of miR-21-3p mimic or negative-control (NC) mimic for 24 h and then incubated with BrdU for an additional 24 h, which is measured using BrdU incorporation assay. The data are represented as the mean±standard deviation for 3 independent experiments. The asterisk mark “*” indicates a significant difference with P<0.05.
FIG. 6C represents the apoptotic cell population of HepG2 cells transfected with negative-control (NC) mimic for 72 h, which is detected using flow cytometry.
FIG. 6D represents the apoptotic cell population of HepG2 cells transfected with 50 nM of miR-21-3p mimic for 72 h, which is detected using flow cytometry.
FIG. 6E shows the quantified comparison of FIGS. 6C and 6D. The data are represented as the mean±standard deviation for 3 independent experiments. The asterisk mark “*” indicates a significant difference with P<0.05.
FIG. 7A represents the microRNAs that change >1.5 fold in PHH cells at 2 h following 40 μM berberine treatment (compared with the untreated PHH cells), which is obtained from microarray results. Only the expression of hsa-miR-21-3p increases in all PHH cells obtained from the 3 donors.
FIG. 7B represents the mRNA expression levels of hsa-miR-21-3p in PHH cells at 2 h following 40 μM berberine treatment and untreated PHH cells (CTL), which is measured by qRT-PCR. The data are represented as the mean±standard deviation for independent experiments using PHH cells from 3 donors. *P<0.05.
FIG. 7C represents the intracellular lipid droplet contents in PHH cells transfected with 50 nM of hsa-miR-21-3p mimic or negative-control (NC) mimic for 72 h, which are shown in folds. The data are represented as the mean±standard deviation for independent experiments using PHH cells from 3 donors. *P<0.05.
FIG. 7D represents the experimental procedures using HepG2 cells as the in vitro liver model for the fat droplet formation induced by oleic acid.
FIG. 7E represents intracellular lipid droplet contents in HepG2 cells induced by 200 μM oleic acid to form fat droplet and the untreated HepG2 cells. The data are represented as the mean±standard deviation for 3 independent experiments. The asterisk mark “*” indicates a significant difference with P<0.05.
FIG. 7F represents intracellular lipid droplet contents in HepG2 cells induced by oleic acid to form fat droplet and then treated with 40 μM berberine treatment for 72 h (compared with the untreated HepG2 cells). The data are represented as the mean±standard deviation for 3 independent experiments. The asterisk mark “*” indicates a significant difference with P<0.05.
FIG. 7G represents intracellular lipid droplet contents in HepG2 cells induced by oleic acid to form fat droplet, transfected with 100 nM miR-21-3p hairpin inhibitor or negative-control (NC) inhibitor for 24 h, and then treated with 40 μM berberine treatment for 48 h (compared with the untreated HepG2 cells). The data are represented as the mean±standard deviation for 3 independent experiments. The asterisk mark “*” indicates a significant difference with P<0.05.
FIG. 7H represents intracellular lipid droplet contents in HepG2 cells induced by oleic acid to form fat droplet, transfected with 50 nM miR-21-3p mimic or negative-control (NC) mimic for 72 h. The data are represented as the mean±standard deviation for 3 independent experiments. The asterisk mark “*” indicates a significant difference with P<0.05.
FIG. 8 represents the mRNA expression levels of lipid metabolic genes in PHH cells stimulated by 40 μM berberine treatment for 2 h, 4 h and 8 h (compared with the untreated PHH cells, CTL), in which the data is shown in folds. The data are represented as the mean±standard deviation for independent experiments using PHH cells from 4 donors. The asterisk mark “*” indicates a significant difference with P<0.05.
FIG. 9A represents the mRNA expression levels of ACACA and DGAT2 in PHH cells stimulated by 40 μM berberine treatment for 2 h, 4 h and 8 h (compared with the untreated PHH cells, CTL), in which the data is shown in folds. The data of ACACA are represented as the mean±standard deviation for independent experiments using PHH cells from 4 donors; and the data of DGAT2 are represented as the mean±standard deviation for independent experiments using PHH cells from 3 donors. The asterisk mark “*” indicates a significant difference with P<0.05.
FIG. 9B represents the mRNA expression levels of ACACA, ACACB and DGAT2 in PHH cells transfected with 50 nM miR-21-3p mimic or negative-control (NC) mimic for 24 h and 48 h, in which the data is shown in folds. The data are represented as the mean±standard deviation for independent experiments using PHH cells from 3 donors. The asterisk mark “*” indicates a significant difference with P<0.05.
FIG. 9C represents the mRNA expression levels of ACACA, ACACB and DGAT2 in HepG2 cells transfected with 100 nM miR-21-3p hairpin inhibitor or negative-control (NC) inhibitor for 24 h, and then stimulated with 40 μM berberine treatment for 0-8 h, in which the data is shown in folds. The data are represented as the mean±standard deviation for independent experiments using PHH cells from 3 donors. The asterisk mark “*” indicates a significant difference with P<0.05.
FIG. 9D represents the mRNA expression levels of ACACA, ACACB and DGAT2 in HepG2 cells transfected with 50 nM miR-21-3p mimic or negative-control (NC) mimic for 24 h, in which the data is shown in folds. The data are represented as the mean±standard deviation for independent experiments using PHH cells from 3 donors. The asterisk mark “*” indicates a significant difference with P<0.05.
FIG. 9E represents the protein expression levels of ACACA, ACACB and DGAT2 in HepG2 cells transfected with 50 nM miR-21-3p mimic or negative-control (NC) mimic for 72 h.
FIG. 9F represents the intracellular malonyl-CoA concentration in HepG2 and PHH cells transfected with 50 nM miR-21-3p mimic or negative-control (NC) mimic for 72 h, in which the data is shown in folds. The data are represented as the mean±standard deviation for independent experiments using PHH cells from 3 donors. *P<0.05.



DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS
In the present invention, the data obtained by miRNA microarray analysis have proved that the expression level of hsa-miR-21-3p increased after berberine treatment in human hepatoma cell line (HepG2). We integrated the gene expression profiles of HepG2 cells after berberine treatment and the gene list that we generated from sequence-based miRNA target prediction software to obtain the putative targets of miR-21-3p, i.e. MAT2A, DIDO1, EEF2K, NBPF8 and TMEM137. The following experiments prove that miR-21-3p inhibits the expression of MAT2A directly, and it also targets to the 3′ UTR of MAT2B to inhibit the expression of MAT2B. In addition, the over-expression of miR-21-3p increase intracellular SAM contents, which has been proven to reduce the cell growth of hepatoma. We have also found that miR-21-3p reduces the expression of EEF2K. EEF2K has been reported as a putative target for anti-cancer therapy because it promotes the growth of tumor and resists to cell apoptosis[55]. Therefore, miR-21-3p over-expression will inhibit the cell growth of HepG2 and induces apoptosis. The results show that miR-21-3p works as a tumor suppressor and potential for treating HCC.
Cell Culture and Treatment
The human HepG2 cells and HEK 293T cells used in the following examples were originally obtained from the American Tissue Culture Collection (ATCC, USA). The human HCC HepG2 cell line was cultured in Minimum Essential Medium Eagle (Sigma) supplemented with 10% fetal bovine serum (FBS) (Invitrogen), 2.2 g/L of sodium bicarbonate (Sigma), 0.1 mM of non-essential amino acids (Caisson), 1 mM of sodium pyruvate (Invitrogen), and 10 of ml/L penicillin-streptomycin-amphotericin solution (Biological Industries). Human HEK-293T cells were maintained in high-glucose Dulbecco's modified eagle medium (Invitrogen) supplemented with 10% FBS and 3.7 g/L of sodium bicarbonate (Sigma). Both cell lines were cultured at 37° C. and 5% CO2.
The primary human hepatocytes from Caucasian donors were purchased from Invitrogen and cultured following the manufacturer's instructions. The hepatocytes were thawed and transferred into cryopreserved hepatocytes recovery medium (CM7000, Invitrogen), centrifuging at room temperature, 100×g for 10 min. Then, the hepatocytes were diluted to seeding density with plating medium (CM3000, Invitrogen) and added into collagen I-coated plates (Invitrogen). These plates were incubated at 37° C. for 4-6 hours under 5% CO2 for forming a monolayer. Then, plating medium was replaced by overlay incubation medium (CM4000 and 0.35 mg/mL GeltrexTM Matrix, Invitrogen), and the incubation medium was replaced daily. In the following examples, hepatocytes form 3 to 4 donors were used for experiments.
A 50 mM stock solution of berberine chloride (Sigma-Aldrich) was prepared in dimethyl sulfoxide (DMSO). Cells were treated with 40 μM of berberine chloride or 0.08% DMSO as the control. For induction of lipid droplets formation in cultured cells, 200 μM of oleic acid (Sigma-Aldrich) was added into the culture medium for 24 h.
RNA Isolation
Total RNA was extracted using a TRIZOL reagent (Invitrogen) according to the manufacturer's protocol. The total RNA quantity was measured using a NanoDrop ND-1000 spectrophotometer (Nanodrop Technologies). The total RNA quality and integrity were assayed using an Agilent 2100 bioanalyzer with an RNA 6000 nano kit (Agilent Technologies).
Microarray
The miRNA profiling was performed using an Agilent human miRNA Microarray R14 V2 containing 866 human miRNAs. The labeling and hybridization of total RNA were performed by following the standard protocol of Agilent's miRNA microarray system of SurePrint G3 human GE 8×60K microarrays (Agilent Technologies). Microarrays were scanned following the Agilent microarray scanner protocol, and image analysis and quantification were performed using the Agilent Feature Extraction software (Agilent Technologies). GeneSpring Gx software (Agilent Technologies) was used to identify miRNAs that were differentially expressed (fold-change >2) between the berberine-treated and untreated samples. The gene expression microarray was performed using a HumanHT-12 v4 Expression BeadChip (Illumina). The labeled cRNA was generated from an RT-IVT Kit (Ambion) and a TotalPrep RNA amplification kit (Illumina). The labeled cRNA was then hybridized to microarrays following the manufacturer's protocol. GenomeStudio software (Illumina) was used to identify miRNAs that were differentially expressed (fold-change >1.5 and P<0.05) between the berberine-treated and untreated samples. All microarray data were deposited in the NCBI GEO database (GSE47822 and GSE53416).
Quantitative Real-Time RT-PCR (qRT-PCR)
(1) MicroRNA Assays
Total RNAs (1 ng) were reverse-transcribed into cDNA by using TaqMan Small RNA Assays kits with hsa-miR-21-3p-, hsa-miR-21-5p- or RUN6B-specific RT primers (Invitrogen). The microRNA expression levels were normalized to RNU6B levels.
(2) Gene Expression Assays
Total RNAs (1 μg) were reverse-transcribed into cDNA by using M-MuLV Reverse Transcriptase (Thermo) and Oligo(dT)12-18 primers (Invitrogen) according to the manufacturer's protocol. The cDNA were then used for a real-time PCR with a LightCycler 480 SYBR Green I Master (Roche) by using primers shown in the following Table 1. The gene expression levels were normalized to GAPDH levels.







TABLE 1 





primers used for gene expression detection
















MAT1A
5′-GCCAAGGGCTTTGACTTC-3′ (SEQ ID No: 1)


primers
5′-CTGTCTCGTCGGTAGCATA-3′ (SEQ ID No: 2)


 


MAT2A
5′-ACAATCTACCACCTACAGCC-3′ (SEQ ID No: 3)


primers
5′-CCAACGAGCAGCATAAGC-3′ (SEQ ID No: 4)


 


MAT2B
5′-TGGTTTCAGAAGAGCAAGAC-3′ (SEQ ID No: 5)


primers
5′-ATTCCCAGAAGCATCCAC-3′ (SEQ ID No: 6)


 


DIDO1
5′-GATGAGGAGCCTGGAGAC-3′ (SEQ ID No: 7)


primers
5′-AGAAATGCCCACACAATCG-3′ (SEQ ID No: 8)


 


EF2K
5′-GGCAAACTCCTTCCACTTCA-3′ (SEQ ID No: 9)


primers
5′-CATCATCCAGCCATTCCC-3′ (SEQ ID No: 10)


 


NBPF8
5′-CAGGACATCGGTGGAATCA-3′ (SEQ ID No: 11)


primers
5′-CTTCTGTAGGGCTGGCAT-3′ (SEQ ID No: 12)


 


TMEM137
5′-GAAGACTGGTTGAGTGGGAT-3′ (SEQ ID No: 13)


primers
5′-TGTCACAGGCAAGTTCACAT-3′ (SEQ ID No: 14)


 


ACACB
Hs_ACACB_1_SG QuantiTect primer assay


primers
QT00996352primer (Qiagen)


 


HLCS
5′-AGTCAGTCAAGTTTGCGTC-3′ (SEQ ID No: 15)


primers
5′-GAGTCGGAGCCCACATAGA-3′ (SEQ ID No: 16)


 


MTOR
5′-CGTCCCTACCTTCTTCTTCC-3′ (SEQ ID No: 17)


primers
5′-TACCACTGAGGCTTCTGC-3′ (SEQ ID No: 18)


 


RPTOR
5′-ACTTCCCGCTCAGAGTTAGA-3′ (SEQ ID No: 19)


primers
5′-CGAGAACCTCCAGCCTTA-3′ (SEQ ID No: 20)


 


ACSM2A
5′-CTCCGCAACTTAGGATGGG-3′ (SEQ ID No: 21)


primers
5′-CTCTCTCTGTCTCTCTCTCG-3′ (SEQ ID No: 22)


 


NAMPT
5′-GCAAGTCTGTTGGTGCTAT-3′ (SEQ ID No: 23)


primers
5′-TTATCTGGGTGTGCCCTG-3′ (SEQ ID No: 24)


 


IL6R
5′-GTGGTAGCCGAGGAGGAA-3′ (SEQ ID No: 25)


primers
5′-GGTCAGAGTCACGCTGTC-3′ (SEQ ID No: 26)


 


SREBF2
5′-TACCTTCCTTCTCTCCCTCG-3′ (SEQ ID No: 27)


primers
5′-GTGGTGCTGAATGTTGGC-3′ (SEQ ID No: 28)


 


ACACA
5′-TTTGTGCCACGGTTATCAT-3′ (SEQ ID No: 29)


primers
5′-CCAAGTAATAGCCAGACTCG-3′ (SEQ ID No: 30)


 


DGAT2
5′-GAAGTTCCCAGGCATACG-3′ (SEQ ID No: 31)


primers
5′-GACCACGATGATGATAGCATTG-3′ 



(SEQ ID No: 32)


 


GAPDH
5′-GGTATCGTGGAAGGACTCAT-3′ (SEQ ID No: 33)


primers
5′-CCTTGCCCACAGCCTTG-3′ (SEQ ID No: 34)









Transfection of MicroRNA Mimics and Inhibitors

The present invention also uses miRNA mimics and inhibitors for transfection experiments. miRNA mimics are used to observe the effects induced by the miRNA, and miRNA inhibitors are used to inhibit the expression of endogenous miRNA in cells to make the expression of target gene stronger.
All of the miRNA mimics and inhibitors were purchased from Thermo Scientific Dharmacon, comprising:













hsa-miR-
CAACACCAGUCGAUGGGCUGU (SEQ ID No: 35)


21-3p
The name shown in miRNA database is


mimic
hsa-miR-21-3p (No. MIMAT0004494)


 


NC mimic
UCACAACCUCCUAGAAAGAGUAGA 



(SEQ ID No: 36)



The name shown in miRNA database is 



Cel-mir-67 (No. MIMAT0000039)


 


hsa-miR-
miRIDIAN Hairpin Inhibitor 


21-3p
hsa-miR-21-3p (purchased from 


inhibitor
Thermo Scientific Dharmacon), an RNA



sequence which can bind 



SEQ ID No: 35


 


NC 
miRNA Hairpin Inhibitor N-ctrl #1 


inhibitor
(purchased from Thermo Scientific 



Dharmacon), an RNA sequence which 



can bind SEQ ID No: 36









The HepG2 cells were transfected at a density of 5×104 cells per well in a 24-well culture plates with either 50 nM of hsa-miR-21-3p (miR-21-3p mimic) or negative-control mimic (NC mimic), or with either 100 nM of hsa-miR-21-3p inhibitor (miR-21-3p inhibitor) or negative-control inhibitor (NC inhibitor) by using the DharmaFECT 4 transfection reagent (Thermo Scientific Dharmacon) according to the manufacturer's instructions. Cells were incubated for 24 h or 48 h with a microRNA mimic or inhibitor prior to RNA purification for gene expression analysis, and were incubated for 72 h for protein expression analysis.
Western Blotting
Total cell lysates were prepared using a lysis buffer (7 M of urea, 4% CHAPS, 2 M of thiourea, 40 mM of Tris, 65 mM of dithioerythritol). Protein samples were separated using 12.5% SDS-PAGE and then transferred to PVDF membranes. The following primary antibodies were used: rabbit polyclone anti-MAT1A (1:800, GeneTex), anti-MAT2A (1:1000, GeneTex), anti-MAT2B (1:1000, GeneTex), anti-ACACB (1:1000, Sigma), anti-DGAT2 (1:1000, GeneTex) and anti-GAPDH (1:3000, GeneTex) and mouse monoclone anti-ACACA (1:1000, Millipore). The goat polyclonal anti-rabbit or anti-mouse IgG antibody conjugated with HRP (1:5000, abcam) was used as the secondary antibody. The bands were imaged using the LAS-4000 mini luminescent image analyzer (Fujifilm). The quantification of western blot analysis was achieved by using Image J software. The protein expression levels were normalized to the GAPDH levels.
Construction of the Luciferase Reporter Plasmids
Full-length 3′ UTRs of MAT2A and MAT2B were amplified from the genomic DNA of HepG2 cells through PCR.







TABLE 3 





primers used to amplify 3′ UTR sequences
















MAT2A
forward primer with a SpeI restriction 


3′ UTR
site


Primers
5′-ATAACTAGTGTGTTAGCCTTTTTTCCCCAG-3′



(SEQ ID No: 37)



reverse primer with a HindIII restriction



site



5′-ATAAAGCTTGCACTTTCTGCTTAGGGCAA-3′



(SEQ ID No: 38)


 


MAT2B
forward primer with a MluI restriction 


3′ UTR
site


primers
5′-ATAACGCGTTGGCACTTTTTAAAGAACAAAGG-3′



(SEQ ID No: 39)



reverse primer with a HindIII restriction



site



5′-ATAAAGCTTAAAAATTAAAGCAACAAAAGAACAA-3′



(SEQ ID No: 40)









The PCR products were then cloned into the pMIR-REPORT Luciferase miRNA Expression Reporter Vector (Invitrogen), and all inserted sequences in the 3′ UTR constructs were checked using the ABI PRISM DNA sequencer.
Mutagenesis and Plasmid Construction
The mutagenesis of the target sequences of hsa-miR-21-3p in MAT2A and MAT2B 3′ UTRs was performed using the QuikChange site-directed mutagenesis kit (Agilent) according to the manufacturer's standard protocol.







TABLE 4 





primers used for sited-mutagenesis in 3′


UTR sequences















For the mutagenesis of Site 1 (+180-200) in the


3′ UTR of MAT2A


5′-CAGCTCTGCCCTCCCTTCTGTTGATATCAGCCAGACCCC-3′


(SEQ ID No: 41)


 


For the mutagenesis of Site 2 (+1267-1288) in the


3′ UTR of MAT2A


5′-CACTAAATTCATTATAATGGTGAACAAGATATCTAGGGACAGA


ATAGCAAGCCCAACT-3′


(SEQ ID No: 42)


 


For the mutagenesis of the target site (+399-418)


in the 3′ UTR of MAT2B


5′-TTTGATCTGAGCTCAGGCAAAGCAAATAATGGATATCAATGAT


TTTTATACTATTTCACACAATTTAA-3′


(SEQ ID No: 43)









All mutated sequences, including Site 1, Site 2 and the double mutation of the MAT2A and the 3′ UTR mutant construct of MAT2B, were checked through DNA sequencing.
Dual Luciferase Reporter Assay
The HEK-293T and HepG2 cells were seeded at a density of 5×104 cells per well in a 24-well culture plate the day before transfection. The HEK-293T and HepG2 cells were tri-transfected with (1) any one of the above-mentioned pMIR-REPORT-3′ UTR construction (300 ng), (2) the control Renilla luciferase reporter plasmid pRL-TK from Promega (10 ng), and (3) 50 nM of hsa-miR-21-3p mimic or negative-control mimic by using the Lipofectamine 2000 transfection reagent (Invitrogen) according to the manufacturer's instructions. Luciferase assays were performed after transfection for 48 h by using the Dual Luciferase reporter assay kit (Promega) and the Paradigm detection platform (Beckman) according to the manufacturer's protocol. The firefly luciferase activity was normalized relative to the Renilla luciferase activity.
Measurement of Intracellular SAM Concentration
After transfection with 50 nM of hsa-miR-21-3p mimic or the negative-control mimic for 72 h, the HepG2 cells were trypsinized and counted. The intracellular SAM of 5×104 HepG2 cells pellet was resuspended in 30 mL of an extraction solution (0.2% perchloric acid plus 0.08% (v/v) 2-mercaptoethanol in ddH2O). Cells were incubated at room temperature for 1 h and vortexed every 5 min. The suspension was centrifuged at 4° C. at 10000×g for 5 min, and then the supernatant was collected. SAM levels in the supernatants were quantified using the Bridge-It SAM fluorescence assay kit (Mediomics) and detected using SpectraMax plate reader (Molecular Devices) according to the manufacturer's instructions.
Cell Proliferation Assay
The HepG2 cells were seeded at a density of 1×104 cells per well in a 96-well culture plate the day before transfection. After transfection with 50 nM of hsa-miR-21-3p mimic or the negative-control mimic for 24 h, the cultured media were refreshed with complete media containing the BrdU reagent, and incubated for an additional 24 hour for BrdU incorporation. The BrdU incorporation was quantified by using the BrdU cell proliferation colorimetric ELISA kit (abcam) according to the manufacturer's protocol and detected using the MRX II microplate reader (DYNEX).
Detection of Apoptosis and Intracelluar Lipid Droplet Contents by Using Flow Cytometry
After transfection with 50 nM of hsa-miR-21-3p mimic or the negative-control mimic for 72 h, the HepG2 cells were trypsinized and counted. Apoptosis was detected by measuring the sub-G1 population by using flow cytometry with propidium iodide (PI) staining. In brief, the cells were fixed in 70% ethanol on ice for 15 min, and stained with the PI staining solution (20 μg/mL PI, 0.1% Triton-X 100, and 0.2 mg/mL RNase A in PBS) for 30 min at room temperature and analyzed using CyAn ADP (Beckman Coulter) flow cytometry with Summit software.
The intracellular lipid droplets were detected by measuring the geometric means of fluorescence intensity using flow cytometry with BODIPY 493/503 (Invitrogen) staining. In brief, the cells were fixed in 70% ethanol at 4° C. for 20 min, and stained with the BODIPY staining solution (10 μg/mL in 70% ethanol) (Invitrogen) for 20 min at room temperature and analyzed using CyAn ADP (Beckman Coulter) flow cytometry with Summit software.
Measurement of Intracellular Malonyl-CoA Concentration
After transfection with 50 nM of miR-21-3p mimic or the negative-control mimic for 72 h, the PHH cells and HepG2 cells were trypsinized. The intracellular malonyl-CoA concentration was measured using the malonyl-CoA ELISA Kit (MyBioSource) according to the manufacturer's instructions.
The following examples are given to illustrate the present invention, and not meant to limit the scope of the present invention. Those skilled in the art will appreciate that the present invention can be practiced by other than the disclosed embodiments. All cited reference are incorporated herein by references.
EXAMPLES
Example 1
Increased miR-21-3p Expression after Berberine Treatment in HepG2 Cell Lines
Because xenobiotic drug-induced miRNAs have recently emerged as key regulators in guiding their pharmacological effects and toxicity[56,57], we examined whether miRNA expression is differentially altered by berberine treatment in HCC
To identify miRNAs induced by berberine treatment, miRNA profiling was performed with an Agilent human miRNA microarray containing probes for 866 human miRNAs. Comparing the miRNA profiles of 40 μM of the berberine-treated HepG2 human hepatoma cell line to those of control cells sampled after 2 h and 4 h of treatment shows that only hsa-miR-21-3p (in which hsa represents human, miR represents mature miRNA, 21 represents the order of discovery, and 3p represents it is the 3p miRNA of the two complementary mature miRNAs, i.e. 3p and 5p, resulted from the pre-miRNA) (previously named as miR-21*) had increased in the HepG2 cell line after berberine treatment (4-fold increase) (FIG. 1A). To further assess the relevance of miR-21-3p in berberine treatment, qRT-PCR assays were used to measure the miR-21-3p expression in HepG2 stimulated by the time course of berberine treatment for up to 8 h compared with the untreated control. As shown in FIG. 1B, miR-21-3p levels started to increase substantially by 1 h (2.1-fold increase) after treatment, peaked at 2 h (3.5-fold increase), and persisted until 8 h (2.8-fold increase). In addition, miR-21-5p (the miRNA formed from the same pre-miRNA and partially complementary to 3p) (previously named as miR-21) increased 2-fold after 2 h of treatment, whereas no significant differences in miR-21-5p expression were detected at 4 h and 8 h. These results show that berberine treatment could significantly up-regulate miR-21-3p expression.
Example 2
Multiple Species Alignments Show that miR-21-3p is Conserved Over the Mammalian Evolution
The final fate of the miRNA* strand, either expressed abundantly as a potential functional guide miRNA or degraded to a passenger strand, may be destined across evolution[54]. Well-conserved miRNA* strands in seed sequences may afford potential opportunities for contributing to the regulation network[52].
As shown in FIG. 2, we analyzed the MIR21 gene with respect to the 18-way alignments of mammalian genomes from the UCSC genome browser, and found that miR-21-3p shows conservation over the mammalian evolution. Based on the results of sequence comparisons, we found that human MIR21 has the closest evolutionary relationships with chimpanzee and rhesus macaque MIR21. Furthermore, one nucleotide substitution is in the seed region of miR-21-3p from humans, chimpanzees, and rhesus macaques compared to the remaining 15 mammals. This one nucleotide substitution, MIR21 (+54 G to C), replaces the fifth nucleotide of the conserved seed region of miR-21-3p, which may alter the regulatory role of miR-21-3p in humans, chimpanzees and rhesus macaques from remaining 15 mammals.
From FIG. 2, it is found that the seed region sequence of hsa-miR-21-3p, the microRNA mimic of the present invention, is ACCACC (SEQ ID No. 44).
Example 3
MicroRNA-21-3p Target Prediction and Validation
Identifying functionally important target genes of specific miRNA and understanding the mechanisms of their actions are essential to uncovering its biological function[58]. To predict the putative targets of miR-21-3p, we integrated the gene expression profiles of HepG2 cells after berberine treatment and compared them with the gene list that we generated from sequence-based miRNA target prediction software. As shown in FIG. 3A, the mRNA targets of miR-21-3p were predicted using the miRanda algorithm and an mirSVR score threshold of −0.1[59]. The predicted genes were overlapped with the microarray data of negatively regulated genes (more than a 1.5-fold decrease and P<0.05) after 40 μM berberine treatment for 4 h in HepG2 cells. Five gene targets, including MAT2A (methionine adenosyltransferase II, alpha), DIDO1 (death inducer-obliterator 1), EEF2K (eukaryotic elongation factor-2 kinase), NBPF8 (predicted: Homo sapiens neuroblastoma breakpoint family, member 8), and TMEM137 (predicted: Homo sapiens transmembrane protein 137) were identified using this prediction strategy. We next confirmed these predictions by performing gain-of-function and loss-of function experiments with miRNA mimics and inhibitors, and confirmed whether miRNA inhibitors could successfully rescue the berberine function in lowering the expression levels of selected targets. FIG. 3B shows that transfecting 50 nM of miR-21-3p mimics into HepG2 cells for 24 hours resulted in a >50% decrease in the mRNA expression of MAT2A and EEF2K, and a <50% decrease in the mRNA expression of DIDO1, NBPF8 and TMEM137. After the transfection of 100 nM of miR-21-3p hairpin inhibitor or negative-control inhibitor into HepG2 cells for 24 h, the cells were stimulated by the time course of 40 μM berberine treatment for up to 8 h compared with the untreated control. FIG. 3C shows that miR-21-3p inhibitor could successfully rescue the berberine function in the lowering mRNA expression levels of predicted targets. These results suggest that MAT1A, DIDO1, EEF2K, NBPF8, and TMEM137 were the targets of miR-21-3p. In the following experiments, we focused on methionine adenosyltransferase (MAT) which is strongly associated with hepatocellular carcinoma.
Example 4
MicroRNA-21-3p Reduces the Expression of Methionine Adenosyltransferases 2B and 2B
After determining that MAT2A is the putative target of miR-21-3p, we investigated whether the expression levels of MAT family members including MAT1A, MAT2A, and MAT2B, could be altered by miR-21-3p. The histogram in FIG. 4A shows the miRanda-mirSVR scores of each seed complementary site in the 3′ UTRs of MAT1A, MAT2A, and MAT2B. MAT2A and MAT2B scored lower than −0.1, suggesting that MAT2B might also be a target of miR-21-3p. To demonstrate the relationship between miR-21-3p and the expression levels of the MAT family, a miR-21-3p mimic or a negative-control mimic was transfected into HepG2 cells. The mRNA and protein expression levels of the MAT family members were subsequently assayed using qRT-PCR and western blotting. FIG. 4B shows that transfecting 50 nM of miR-21-3p mimic into HepG2 cells for 48 h resulted in a >50% decrease in the mRNA expression of MAT2A and MAT2B, but not in that of MAT1A. Furthermore, after the transfection of hsa-miR-21-3p mimic into HepG2 cells for 72 h, the protein expression levels of MAT2A and MAT2B showed a 2.6-fold and a 3.4-fold decrease, respectively. Furthermore, the MAT1A protein expression levels showed a 1.7-fold increase (FIG. 4C). By contrast, for the loss-of function experiments, the hairpin inhibitor of miR-21-3p were transfected into HepG2 cells to inhibit functions of endogenous miR-21-3p (with a negative control inhibitor as control). As shown in FIG. 4D, the MAT1A was 1.3-fold decreased by miR-21-3p inhibitor after transfection for 24 h, but not for 48 h. The expression levels of MAT2A and MAT2B remained unchanged after transfection for 24 h and 48 h. The MAT2B expression levels did not change notably according to our microarray data shown in FIG. 3A. To assess the relevance of berberine treatment and the expression levels of the MAT family members, qRT-PCR assays were used to measure mRNA expression in HepG2 cells stimulated by the time course of berberine treatment for up to 8 h compared with the untreated control (0.08% DMSO). As shown in FIG. 4E, MAT2A levels started to decreasing substantially by 2 h (1.8-fold decrease) after treatment, reached the lowest value at 4 h (3.8-fold decrease), and remained constant until 8 h (2.2-fold increase). The decreased levels of MAT2A were associated with higher miR-21-3p levels after berberine treatment for 2 h, 4 h, and 8 h, and the comparisons are shown in FIG. 1B. However, MAT1A and MAT2B expression levels did not change substantially which was consistent with our microarray data. Overall, our findings are the first evidence indicating that berberine treatment reduced MAT2A, and that the over-expression of miR-21-3p reduced the expression of both MAT2A and MAT2B.
Example 5
MicroRNA-21-3p Up-Regulates Intracellular SAM Contents in Hepatoma Cells
The increased expression of MAT2A and MAT2B in HCC results in decreasing SAM levels and facilitates cancer cell growth[6,7,15]. After determining that MAT2A and MAT2B are repressed by miR-21-3p, we analyzed the intracellular SAM contents in the HepG2 cell after transfection with miR-21-3p mimic and negative-control mimic as the control for 48 h and 72 h. As shown in FIG. 4F, the intracellular SAM contents were 1.6-fold and 3.0-fold increased by miR-21-3p mimic after transfection for 48 h and 72 h. These results indicate that the over-expression of miR-21-3p raised intracellular SAM contents, which have been proven to impair the growth of hepatoma cells.
Example 6
MAT2A and MAT2B are Direct Targets of miR-21-3p
Because MAT2A and MAT2B decreased after miR-21-3p mimic transfection, we investigated the relevance of miR-21-3p and the 3′ UTRs of MAT2A and MAT2B. Full-length wild-type or mutant 3′ UTRs of MAT2A and MAT2B were separately cloned into luciferase reporter vectors (FIGS. 5A and 5B, respectively), and the dual luciferase reporter assay system was used to quantitate the reporter activity. FIG. 5C shows that miR-21-3p suppressed the expression (a 1.6-fold decrease in HEK 293T cells and a 1.5-fold decrease in HepG2 cells) of the luciferase reporter containing the MAT2A 3′ UTR, suggesting that miR-21-3p directly regulates the MAT2A 3′ UTR. To confirm whether the miR-21-3p cleaves the MAT2A 3′ UTR through miRNA:mRNA seed pairing, the site-directed mutagenesis of the putative miR-21-3p binding sequence on the MAT2A 3′ UTR was performed. By using MicroRNA.org (http://www.microrna.org), a comprehensive resource of microRNA target predictions, miR-21-3p was predicted to target 2 sites (Site 1: +180-200 and Site 2: +1267-1288) in the 3′ UTR of MAT2A. The results show that miR-21-3p suppressed the expression (a 1.5-fold decrease in both HEK 293T cells and HepG2 cells) of the luciferase reporter containing Site 1 mutated MAT2A 3′ UTR. Conversely, the expression levels of the luciferase reporter containing Site 2 mutated MAT2A 3′ UTR did not notably change, similar to the reporter containing the double-mutated (both Site 1 and Site 2) MAT2A 3′ UTR. This suggests that Site 2 (+1267-1288) in the MAT2A 3′ UTR is the major cleavage site of miR-21-3p (FIG. 5C). In addition, miR-21-3p was predicted to target one seed match (+399-418) in the 3′ UTR of MAT2B. As shown in FIG. 5D, miR-21-3p suppressed the expression (a 2.3-fold decrease in HEK 293T cells and a 1.4-fold decrease in HepG2 cells) of the luciferase reporter containing the MAT2B 3′ UTR, but the expression levels of the luciferase reporter containing the mutated MAT2B 3′ UTR remained unchanged in both HEK 293T cells and HepG2 cells. This suggests that the seed match (+399-418) in the MAT2B 3′ UTR is the major cleavage site of miR-21-3p. These results showed that MAT2A and MAT2B are both direct targets of miR-21-3p.
Example 7
MicroRNA-21-3p Suppresses Growth and Induces Apoptosis in Hepatoma Cells
To investigate the potential effects of miR-21-3p on cell growth and viability, we measured cell proliferation and viability by using the BrdU incorporation assay and the Trypan blue dye exclusion assay. The results shown in FIG. 6A indicate that miR-21-3p mimic reduced the viable cell numbers in HepG2 cells after transfection for 48 h. In addition, transfecting miR-21-3p mimic into HepG2 cells for 24 h and incubation for an additional 24 h for BrdU incorporation led to the inhibition of cellular proliferation (1.7-fold), compared with transfection with negative-control mimic (FIG. 6B). The effects of miR-21-3p on apoptosis and the cell cycle were evaluated using flow cytometry analysis. After HepG2 cells transfection with miR-21-3p and negative-control mimic as the control for 72 h, the sub-G1 populations of apoptotic cells were quantified. As shown in FIGS. 6C, 6D, and 6E, miR-21-3p induced apoptosis in HepG2 cells.
Example 8
MicroRNA-21-3p Reduces Lipid Droplet Content and Induces Apoptosis in Hepatic Cells
The isoquinoline alkaloid berberine has a wide range of pharmacological effects, including its lipid-lowering effect in alleviating fatty liver syndrome in vivo[54-58]. Using the method the same as that used in Example 1, we employed a microRNA array to determine whether berberine treatment changed the microRNA among non-transformed human hepatocytes. The microRNA profiles of berberine-treated (40 μM) primary human hepatocytes (PHHs) from 3 overweight or obese male donors were compared with those of control group samples, which were obtained 2 h following treatment. Among the 3 donor microRNA profiles, we observed a consistent increase in only hsa-miR-21-3p expression (mean=3.36-fold; FIG. 7A). The up-regulation of miR-21-3p by berberine treatment in the PHHs was confirmed based on a qRT-PCR (FIG. 7B).
We employed flow cytometry, using BODIPY 493/503 staining[59] to examine the effect of miR-21-3p on the hepatic lipid droplet content. Among the PHH samples, the over-expression of miR-21-3p resulted from transfection of miR-21-3p mimic of the present invention caused a reduction in the intracellular lipid droplet content at 72 h (40% to 44% of the lipid droplet content among the control samples), as shown in FIG. 7C.
The up-regulation of miR-21-3p in PHHs is consistent with the result of Example 1, which shows that miR-21-3p increased in the human HepG2 hepatoma cell line after berberine treatment. Therefore, we used an oleic acid-induced in vitro fatty liver model for HepG2 cells to examine the relationship between hepatic steatosis and berberine-induced miR-21-3p[60, 61]. FIG. 7D shows the timeline for experiment procedures, in which the group 1 was treated with oleic acid for 24 h (1 day) to induce intracellular lipid droplet formation, and then treated with 40 μM berberine for 72 h (3 days); the group 2 was treated with oleic acid for 24 h (1 day) to induce intracellular lipid droplet formation first, then treated with 100 nM miR-21-3p hairpin inhibitor or negative-control (NC) inhibitor for 24 h (1 day), and then treated with 40 μM berberine for 48 h (2 days); and the group 3 was treated with oleic acid for 24 h (1 day) to induce intracellular lipid droplet formation first, and then treated with 50 nM miR-21-3p mimic or negative-control (NC) mimic for 72 h (3 days). After the above treatments, cell samples were collected separately, and the intracellular lipid droplet contents of the HepG2 cells were detected by flow cytometry.
FIG. 7E shows that lipid-accumulated cells mimicking hepatic steatosis were successfully induced in the HepG2 cells by adding oleic acid. In this cell model, the 72-h berberine treatment reduced the lipid droplet content (47% to 57% of the lipid droplet content among the untreated control samples) (FIG. 7F). In addition, FIG. 7G shows that after inhibiting endogenous miR-21-3p by the transfection of miR-21-3p inhibitor, the lipid droplet content cannot be reduced because the miR-21-3p induced by berberine is inhibited by the inhibitor. This demonstrates that berberine induces miR-21-3p to reduce intracellular lipid droplet content. Furthermore, the over-expression of miR-21-3p caused a reduction in the lipid droplet content (50% to 58% of the lipid droplet content among the control group samples) (FIG. 7H). Similar results were obtained in the HepG2 cells, despite a lack of oleic acid-induction (data not shown). Overall, these results indicate that miR-21-3p decreases hepatic lipid droplet content.
Example 9
Prediction and Confirmation of Targets of MicroRNA-21-3p in Primary Liver Cells
To identify which lipid metabolic genes were regulated by berberine, we examined the time course (2, 4, and 8 h) expression profiles of the berberine-treated (40 μM) and untreated PHHs from 3 overweight or obese male donors. The relative mRNA expression levels of the 8 selected genes, including ACACB, HLCS, MTOR, RPTOR, ACSM2A, NAMPT, IL6R and SPEBF2, were further validated (FIG. 8). It was found that the mRNA expression of ACACB, HLCS, MTOR, RPTOR, IL6R, and SPEBF2 decreases during the 4-8-h, and that of ACSM2A considerably decreases during the 2-4 h period. By contrast, an increase in the mRNA expression of NAMPT was observed during the 4-8 h period after berberine treatment. Subsequently, we examined the expression of these genes in HepG2 cells, and found that only ACACB consistently decreased in the HepG2 cells and PHHs (data not shown). Subsequently, we focused on acetyl-CoA carboxylase in the following experiments.
Although ACACA was not extracted from the microarray data, we observed a 2.1-fold decrease in ACACA expression among the 3 donor samples at 8 h after berberine treatment (FIG. 9A). Next, we examined whether miR-21-3p regulated ACACA and ACACB expression. We observed a decrease in both ACACA (1.6-fold) and ACACB (1.8-fold) expression by transfecting the miR-21-3p mimic into the 3 sample PHHs for 48 h (FIG. 9B). In addition, after transfecting of 100 nM of miR-21-3p hairpin inhibitor or negative-control inhibitor into HepG2 cells 24 h, the HepG2 cells were stimulated by the time course of berberine treatment for up to 8 h. Since the miR-21-3p induced by berberine was inhibited by the inhibitor, the mRNA expression levels of ACACA and ACACB are not decreased. This demonstrates that berberine induces miR-21-3p to reduce the mRNA expression levels of ACACA and ACACB (FIG. 9C). Transfection of the miR-21-3p mimic of the present invention into the HepG2 cells resulted in a 44% and a 50% decrease in the mRNA expression of ACACA and ACACB, respectively, at 24 h (FIG. 9D), and resulted in a decrease in the protein expression of ACACA (2.3-fold) and ACACB (1.6-fold), respectively, at 72 h (FIG. 9E). Following the 72-h transfection of the miR-21-3p mimic of the present invention, the intracellular malonyl-CoA concentration exhibited a decrease in both HepG2 cells (1.6-fold) and PHH cells (2.2-fold) (FIG. 9F). These results indicate that the miR-21-3p mimic of the present invention reduces the expression of ACACA and ACACB, and decreased the concentration of intracellular malonyl-CoA.
As for DGAT2, the important enzyme for triacylglycerol biosynthesis, it was predicted as a putative target of miR-21-3p with a miRanda-mirSVR score of −0.2515. The changes in DGAT2 expression levels resulted from berberine treatment were not significant among the PHH cells (FIG. 9A). This was consistent with the microarray data. However, we observed a 1.5-fold decrease in mRNA expression levels of DGAT2 in PHH cells following transfection of the miR-21-3p mimic of the present invention for 48 h (FIG. 9B). When miR-21-3p over-expressed in the HepG2 cells, a 2.3-fold decrease in the mRNA level of DGAT2 was observed at 24 h (FIG. 9D), and a 3.3-fold decrease in the protein level of DGAT2 was observed at 72 h (FIG. 9E).
With the technology similar to that used in Example 6, we subsequently examined the relationship between miR-21-3p and the 3′ UTRs of ACACA, ACACB, and DGAT2, and found that the miR-21-3p suppressed the expression of ACACA, but not though its 3′ UTR sequence (data not shown). The mRNA stability assays showed that berberine induced miR-21-3p, and miR-21-3p reduced the half-life of ACACA mRNA. In addition, no seed pairing occurred between the seed region of miR-21-3p and 3′ UTR of ACACB, and miR-21-3p suppressed the expression of ACACB (a 1.4-fold decrease) by binding the exon 23 of ACACB. However, miR-21-3p did suppress DGAT2 expression (a 1.4-fold decrease) by pairing with the 3′ UTR sequence of DGAT2. These results indicate that the miR-21-3p decreased hepatic lipid droplet accumulation by reducing the expression of ACACA, ACACB and DGAT2 in different ways.
As for other berberine-associated lipid metabolic genes, the transfection of 50 nM of miR-21-3p mimic caused a decrease in MTOR and RPTOR expression at 24 h (1.4-fold and 1.6-fold decrease, respectively), and a decrease in MTOR, RPTOR and HLCS expression at 48 h (2.5-fold, 2.2-fold and 1.5-fold decrease, respectively) following transfection (50 nM). This helps to explain that the miR-21-3p mimic of the present invention improves lipid metabolism.
The miR-21-3p mimic of the present invention targets to the 3′ UTRs of MAT2A and MAT2B and decreases their expression directly. Over-expression of miR-21-3p up-regulates intracellular SAM contents to inhibit cell growth and induce apoptosis in HepG2 cells. In addition, the miR-21-3p mimic of the present invention decreases hepatic lipid droplet accumulation by reducing the half-life of ACACA mRNA and binding the exon 23 of ACACB and the 3′ UTR sequence of DGAT2. Therefore, our results show that miR-21-3p can be a tumor suppressor and therapeutics for fatty liver disease and it is potential for treating liver diseases.
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## Claims

1. A method for decreasing the expression of acetyl-CoA carboxylase 1 or 2 (ACACA or ACACB), diglyceride acyltransferase 2 (DGAT2), or methionine adenosyltransferase 2A or 2B (MAT2A or MAT2B) in a subject having a fatty liver disease, comprising administering an effective amount of a microRNA mimic containing a single strand RNA molecule of hsa-miR-21-3p (SEQ ID NO:35) to said subject.

2. The method according to claim 1, wherein said microRNA mimic is chemically modified for not being degraded by RNase.

3. The method according to claim 1, wherein said microRNA mimic is delivered into target cells by transfection and/or conjugate delivery.

4. The method according to claim 3, wherein said microRNA mimic is delivered into target cells by conjugate delivery and transfection.

5. The method according to claim 3, wherein said conjugate delivery delivers said microRNA mimic into target cells by binding an aptamer or cholesterol with the microRNA mimic.

6. The method according to claim 3, wherein the transfection delivers said microRNA mimic into target cells by using liposome, exosome, nanoparticle or virus.

7. The method according to claim 6, wherein said nanoparticle comprises lipid nanoparticle or polymer nanoparticle.

8. The method according to claim 1, which is administered by injection.

9. The method according to claim 1, wherein said administering decreases the expression of acetyl-CoA carboxylase 1 and 2 (ACACA and ACACB) and diglyceride acyltransferase 2 (DGAT2).

10. The method according to claim 4, wherein said conjugate delivery delivers said microRNA mimic into target cells by binding an aptamer or cholesterol with the microRNA mimic.

11. The method according to claim 4, wherein the transfection delivers said microRNA mimic into target cells by using liposome, exosome, nanoparticle or virus.

12. The method according to claim 1, wherein said administering decreases the expression of methionine adenosyltransferase 2A and 2B (MAT2A and MAT2B).