# Patent

## Title
Quinoxalines and AZA-quinoxalines as CRTH2 receptor modulators

## Abstract

The invention provides certain quinoxalines and aza-quinoxalines of the Formula (I), and their pharmaceutically acceptable salts, wherein J1, J2, R1, R2, R3, R22, Ra, Rb, Rc, Rd, X, Y, b, n, and q are as defined herein. The invention also provides pharmaceutical compositions comprising such compounds, and methods of using the compounds for treating diseases or conditions associated with uncontrolled or inappropriate stimulation of CRTH2 function.

## Background
CROSS-REFERENCE TO RELATED APPLICATIONS
This Application is a U.S. National Stage Application under 35 U.S.C. §371 of PCT Application No. PCT/US2011/065716 filed Dec. 19, 2011 which claims priority under 35 U.S.C. §119(e) of U.S. Provisional Application No. 61/426,886 filed Dec. 23, 2010.


FIELD OF THE INVENTION
The present invention relates to certain quinoxalines and aza-quinoxalines of the Formula (I) (also referred to herein as the “compounds of the Formula (I)”), compositions comprising such compounds, and methods of using such compounds for treating an inflammatory disease, or other disorder mediated by the chemoattractant receptor-homologous molecule expressed on T-helper-type-2 cells (CRTH2).
BACKGROUND OF THE INVENTION
Prostaglandin D2 (PGD2) belongs to a class of chemical mediators which cells synthesize in response to stimuli, such as local tissue damage or hormonal stimuli, or by cellular activation pathways. Cells synthesize PGD2 from arachidonic acid by cyclooxygenase and other specific synthases in the pathway.
Upon stimulation, mast cells release PGD2 in major amounts and this release plays a major role in the etiology of respiratory disease, such as asthma and congestion. PGD2 achieves this effect by binding with either of two G-protein coupled receptors, which are the D-prostanoid (DP) receptor and the CRTH2 receptor. TH-2 cells, eosinophils, and basophils express the CRTH2 receptor, which mediates the chemoattractant effect of PGD2.
Scientific studies support a clear role for PGD2 in an allergic inflammatory response. PGD2 is found at high levels in the bronchoalveolar lavage of asthmatics. Inhalation of PGD2 enhances eosinophilic and lymphocytic airway inflammation in allergic animal models. Evidence obtained by studying CRTH2 knockout mice demonstrates that PGD2 achieves this enhancement by binding to the CRTH2 receptor. Hence, CRTH2 receptor antagonists would be expected to reduce the allergic inflammatory response caused by PGD2, and these compounds would be useful in the treatment or prevention of allergic/immune disorders.
Current drugs of choice for the treatment of chronic inflammatory airway disease, such as asthma or COPD, are synthetic glucocorticoids; examples of these compounds currently indicated for treating these disorders include fluticasone and mometasone. The difficulty with treating patients with this class of compounds is that the compounds possess a number of systemic side-effects; these include adrenal suppression, altered bone metabolism and growth suppression in children. These side effects limit the dose that can be administered on a daily basis to the patient. While a non-steroidal class of therapeutics that inhibit bronchoconstriction exists (CysLT1 antagonists), this class of compounds has limited efficacy in achieving the endpoints of reducing inflammatory and improving in lung function when compared to the glucocorticoids. Therefore, a therapeutic that combines the efficacy of inhaled glucocorticoids without the side effects would be advantageous.
SUMMARY OF THE INVENTION
In one aspect, the present invention provides a compound of the Formula (I):




or a pharmaceutically acceptable salt thereof, wherein

J1 and J2 are independently C(H), C(R1), C(R22), or N wherein the following provisos apply:

(i) no more than one of J1 and J2 is N,
(ii) no more than one of J1 and J2 is C(R22); and
(iii) only one R1 is substituted on the illustrated ring containing J1 and J2;
R1 is selected from the group consisting of:
(i) —C(O)—N(R6a)(R6b),
(ii) —S(O)2—N(R6a)(R6b),
(iii) —C(O)—C(R7a)(R7b)(R7c),
(iv) —N(H)—C(O)—C(R7a)(R7b)(R7c),
(v) —C(O)—O—C(R7a)(R7b)(R7c), and
(vi) —N(H)—S(O)2—C(R7a)(R7b)(R7c);

    
    
        R6a and R6b are independently:
        
            a. H,
            b. C1-C6 alkyl,
            c. C3-C6 alkenyl,
            d. C3-C6 alkynyl,
            e. —O—(C1-C3 alkyl),
            f. -Q-RAH, wherein RAH is phenyl or 5- to 6-membered heteroaryl containing 1 to 2 heteroatoms independently selected from the group consisting of N, O, and S,
            
                and wherein RAH is unsubstituted or substituted with 1 to 5 R8 moieties independently selected from the group consisting of halo, C1-C3 alkyl, C1-C3 alkoxy, C1-C3 fluoroalkyl, —O—(C1-C3 fluoroalkyl), hydroxyl, phenyl, and —CN;
            
            
            Q is selected from the group consisting of a
            
                (a) a bond;
                (b) C1-C6 alkylene, wherein said C1-C6 alkylene is unsubstituted or substituted by 1 to 2 fluoro, C1-C3 alkyl, C1-C3 hydroxyalkyl, or C1-C3 fluoroalkyl; and
                (c)
            
            
        
        
    
    






    
    
        
        
            
            
                 wherein t is 1, 2, 3, or 4;
            
            
            g. -Q-RHC, wherein RHC is
            
                (i) 5- to 7-membered heterocyclyl containing 1 heteroatom selected from the group consisting of N, O, S, S(O), and S(O)2, wherein said heterocyclyl of RHC is optionally fused to a benzene, pyridyl ring; or
                (ii) C3-C7 cycloalkyl, wherein said cycloalkyl of RHC is optionally fused to a benzene or pyridyl ring;
                and wherein RHC is unsubstituted or substituted with 1 to 5 R12 moieties independently selected from the group consisting of halo, C1-C3 alkyl, C1-C3 alkoxy, C1-C3 fluoroalkyl, —O—(C1-C3 fluoroalkyl), hydroxyl, —CN, and —S(O)2—(C1-C3 alkyl), or wherein when two R12 moieties are geminally substituted on the same carbon atom, the two geminally substituted R12 moieties, together with the carbon atom on which they are attached form —C(O)—;
            
            
            h. or R6a and R6b together with the N atom to which they are attached form R6H, wherein R6H is independently selected from the group consisting of:
            
                (i) a 4- to 9-membered heterocyclyl, optionally containing one additional nitrogen atom, wherein said heterocyclyl of R6H is optionally fused to phenyl, C3-C6 cycloalkyl, or a 5-membered heteroaryl containing 1 to 3 N atoms;
                (ii) a 4- to 7-membered heterocyclenyl, optionally containing one additional nitrogen atom, wherein said heterocyclenyl of R6H is optionally fused to phenyl; and
                (iii) a 6- to 8-membered aza- or a diazabicycloheterocycloalkyl ring;
                wherein R6H is unsubstituted or substituted by 1 to 5 R9 moieties wherein each R9 moiety is independently C1-C6 alkyl, C1-C6 alkoxy, C1-C3 fluoroalkyl, fluoro, hydroxyl, —CN, —(C1-C3 alkylene)-(C1-C3 alkoxy), or
                
                    R9 is —Z—RCY wherein
                     Z is
                     (i) a bond,
                     (ii) —C(O)—,
                     (iii) —C(═N—OH)—,
                     (iv) —S(O)2—,
                     (v) C1-C3 alkylene, wherein said C1-C3 alkylene of Z is optionally substituted by 1 to 2 fluoro or C1-C3 alkyl;
                     (vi) —O—;
                     (vii) —O—(C1-C3 alkylene)-; or
                     (viii) —C(O)—O—CH2 
                     —RCY is selected from the group consisting of
                     (i) phenyl
                     (ii) 5- to 10-membered mono or bicyclic heteroaryl containing 1 to 3 heteroatoms independently selected from the group consisting of N, O, and S; or
                     (iii) 5- to 6-membered heterocyclyl containing 1 to 2 N atoms or 1 O atom, wherein said heterocyclyl of RCY is optionally fused to phenyl;
                     wherein RCY is unsubstituted or substituted by 1 to 4 R10 moieties;
                     each R10 moiety is independently C1-C3 alkyl, halo, hydroxyl, C1-C3 alkoxy, C1-C3 fluoroalkyl, —(C1-C3 alkylene)-(C1-C3 alkoxy), —S(O)2—(C1-C3 alkyl), —C(O)—(C1-C3 alkyl), —CN, pyridyl, or cyclopropyl or, wherein when two R10 moieties are geminally substituted on a common carbon atom, together with the carbon atom on which they are substituted, form
                     —C(O)—;
                
                
                or, optionally, where two R9 moieties are geminally substituted on a common ring carbon of R6H, the two R9 moieties, together with the ring carbon on which they are substituted, form RYC, wherein RYC is
                
                    (i) a 4- to 7-membered cycloalkyl, wherein said cycloalkyl of RYC is optionally fused to phenyl or pyridyl; or
                    (ii) a 4- to 7-membered heterocyclyl containing 1 to 2 N atoms or 1 O atom, wherein said heterocyclyl of RYC is optionally fused to phenyl;
                    wherein RYC is unsubstituted or substituted by 1 to 4 R11 moieties;
                    each R11 moiety is independently C1-C3 alkyl, halo, hydroxyl, C1-C3 alkoxy, —(C1-C3 alkylene)-(C1-C3 alkoxy), —S(O)2—(C1-C3 alkyl), —C(O)—(C1-C3 alkyl), phenyl, or pyridyl, or, wherein when two R11 moieties are geminally substituted on a common carbon atom, together with the carbon atom on which they are substituted, form —C(O)—;
                
                
            
            
        
        
        R7a and R7b are independently
        
            a) H,
            b) C1-C6 alkyl,
            c) R7a and R7b together with the carbon atom on which they are substituted, form RPC, wherein RPC is
            
                (i) C3-C7 cycloalkyl, or
                (ii) phenyl, wherein said phenyl of RPC is unsubstituted or substituted by 1 to 5 moieties independently selected from the group consisting of halo, trifluoromethyl, and trifluoromethoxy;
            
            
        
        
        R7c is
        
            a) H, or
            b) absent, when R7a and R7b together with the carbon atom on which they are substituted form phenyl;

R22 is halo, C1-C3 alkyl, or C1-C3 fluoroalkyl;

b is 0 or 1;

X is a bond, —O—, —S—, —S(O)—, —S(O)2—, or N(H);

Y is selected from the group consisting of

        
        
    
    


(i) a bond,




(iv) —O—, (v) —S—, (vi) —S(O)—, and (vii) —S(O)2—;

    
    
        wherein
        
            a is 0, 1, 2, or 3;
            r is 0, 1, or 2;
            s is 0, 1, or 2;
            each occurrence of R4 is independently halo, C1-C6 alkyl, or C1-C6 fluoroalkyl;
        
        
    
    
    Ra, Rb, Re, and Rd are independently H, fluoro, hydroxyl, C1-C6 alkyl, C1-C6 fluoroalkyl, or C1-C6 alkoxy;
    R2 is selected from the group consisting of:
    
        (i) phenyl;
        (ii) 5- to 6-membered heteroaryl containing from 1 to 3 heteroatoms selected from the group consisting of N, O, and S;
        (iii) 5- to 6-membered heterocyclenyl, containing from 1 to 2 heteroatoms selected from the group consisting of N, O, and S; and
        (iv) 5- to 6-membered heterocyclyl containing from 1 to 2 heteroatoms selected from the group consisting of N, O, and S;
        wherein R2 is unsubstituted or substituted by 1 to 5 R5 groups independently selected from the group consisting of halo, C1-C3 fluoroalkyl,
        C1-C3 alkyl, C1-C3 alkoxy, —CN, —OCF3, —C(O)—(C1-C3 alkyl), and
        —S(O)2—(C1-C3 alkyl);

R3 is —C(O)OH,

    
    






or —N(H)—SO2—Re,

wherein Re is C1-C6 alkyl, C1-C6 fluoroalkyl, C1-C6 alkoxy, and phenyl;
n is 1, 2, 3, 4, or 5; and
q is 0, 1, or 2.
In another aspect, the present invention provides a compound of the Formula (I) or a pharmaceutically acceptable salt thereof, wherein
R1 is selected from the group consisting of:

    
    
        (i) —C(O)—N(R6a)(R6b),
        (ii) —S(O)2—N(R6a)(R6b),
        (iii) —C(O)—C(R7a)(R7b)(R7c),
        (iv) —N(H)—C(O)—C(R7a)(R7b)(R7c),
        (v) —C(O)—O—C(R7a)(R7b)(R7c), and
        (vi) —N(H)—S(O)2—C(R7a)(R7b)(R7c);
        
            R6a and R6b are independently:
            
                a. H,
                b. C1-C6 alkyl,
                c. C3-C6 alkenyl,
                d. C3-C6 alkynyl,
                e. —O—(C1-C3 alkyl),
                f. -Q-RAH, wherein RAH is phenyl or 5- to 6-membered heteroaryl containing 1 to 2 heteroatoms independently selected from the group consisting of N, O, and S,
                
                    and wherein RAH is unsubstituted or substituted with 1 to 5 R8 moieties independently selected from the group consisting of halo, C1-C3 alkyl, C1-C3 alkoxy, C1-C3 fluoroalkyl, —O—(C1-C3 fluoroalkyl), hydroxyl, phenyl, and —CN;
                
                
                Q is selected from the group consisting of a
                
                    (a) a bond;
                    (b) C1-C6 alkylene, wherein said C1-C6 alkylene is unsubstituted or substituted by 1 to 2 fluoro, C1-C3 alkyl, C1-C3 hydroxyalkyl, or C1-C3 fluoroalkyl; and
                    (c)
                
                
            
            
        
        
    
    






    
    
        
        
            
            
                
                
                     wherein t is 1, 2, 3, or 4;
                
                
                g. -Q-RHC, wherein RHC is
                
                    (i) 5- to 6-membered heterocyclyl containing 1 heteroatom selected from the group consisting of N and O, wherein said heterocyclyl of RHC is optionally fused to a benzene ring; or
                    (ii) C5-C7 cycloalkyl, wherein said cycloalkyl of RHC is optionally fused to a benzene ring;
                    and wherein RHC is unsubstituted or substituted with 1 to 5 R12 moieties independently selected from the group consisting of halo, C1-C3 alkyl, C1-C3 alkoxy, C1-C3 fluoroalkyl,
                     —O—(C1-C3 fluoroalkyl), hydroxyl, and —CN, or wherein when two R12 moieties are geminally substituted on the same carbon atom, the two geminally substituted R12 moieties, together with the carbon atom on which they are attached form —C(O)—;
                
                
                h. or R6a and R6b together with the N atom to which they are attached form R6H, wherein R6H is independently selected from the group consisting of:
                
                    (i) a 4- to 7-membered heterocyclyl, optionally containing one additional nitrogen atom, wherein said heterocyclyl of R6H is optionally fused to phenyl; and
                    (ii) a 4- to 7-membered heterocyclenyl, optionally containing one additional nitrogen atom, wherein said heterocyclenyl of R6H is optionally fused to phenyl;
                    wherein R6H is unsubstituted or substituted by 1 to 5 R9 moieties wherein each R9 moiety is independently C1-C6 alkyl, C1-C6 alkoxy, fluoro, hydroxyl, —CN, —(C1-C3 alkylene)-(C1-C3 alkoxy), or
                     R9 is —Z—RCY wherein
                     Z is
                     (i) a bond,
                     (ii) —C(O)—,
                     (iii) —C(═N—OH)—,
                     (iv) —S(O)2—, or
                     (v) C1-C3 alkylene, wherein said C1-C3 alkylene of Z is optionally substituted by 1 to 2 fluoro or C1-C3 alkyl;
                     RCY is selected from the group consisting of:
                     (i) phenyl
                     (ii) 5- to 10-membered mono or bicyclic heteroaryl containing 1 to 3 heteroatoms independently selected from the group consisting of N, O, and S; or
                     (iii) 5- to 6-membered heterocyclyl containing 1 to 2 N atoms or 1 O atom, wherein said heterocyclyl of RCY is optionally fused to phenyl;
                     wherein RCY is unsubstituted or substituted by 1 to 4 R10 moieties;
                     each R10 moiety is independently C1-C3 alkyl, halo, hydroxyl, C1-C3 alkoxy, —(C1-C3 alkylene)-(C1-C3 alkoxy), —S(O)2—(C1-C3 alkyl), —C(O)—(C1-C3 alkyl), —CN, or pyridyl, or, wherein when two R10 moieties are geminally substituted on a common carbon atom, together with the carbon atom on which they are substituted, form —C(O)—;
                    or, optionally, where two R9 moieties are geminally substituted on a common ring carbon of R6H, the two R9 moieties, together with the ring carbon on which they are substituted, form RYC, wherein RYC is
                     (i) a 4- to 7-membered cycloalkyl, wherein said cycloalkyl of RYC is optionally fused to phenyl; or
                     (ii) a 4- to 7-membered heterocyclyl containing 1 to 2 N atoms or 1 O atom, wherein said heterocyclyl of RYC is optionally fused to phenyl;
                     wherein RYC is unsubstituted or substituted by 1 to 4 R11 moieties;
                     each R11 moiety is independently C1-C3 alkyl, halo, hydroxyl, C1-C3 alkoxy, —(C1-C3 alkylene)-(C1-C3 alkoxy), —S(O)2—(C1-C3 alkyl), —C(O)—(C1-C3 alkyl), phenyl, or pyridyl, or, wherein when two R11 moieties are geminally substituted on a common carbon atom, together with the carbon atom on which they are substituted, form —C(O)—;
                
                
            
            
            R7a and R7b are independently
            
                a) H,
                b) C1-C6 alkyl,
                c) R7a and R7b together with the carbon atom on which they are substituted, form RPC, wherein RPC is
                
                    (i) C3-C7 cycloalkyl, or
                    (ii) phenyl, wherein said phenyl of RPC is unsubstituted or substituted by 1 to 5 moieties independently selected from the group consisting of halo, trifluoromethyl, and trifluoromethoxy;
                
                
            
            
            R7c is
            
                a) H, or
                b) absent, when R7a and R7b together with the carbon atom on which they are substituted form phenyl; and

J1, J2, Y, Ra, Rb, Rc, Rd, R2, R3, R22, b, n, and q are as specified in claim 1.

            
            
        
        
    
    




DETAILED DESCRIPTION OF THE INVENTION
Definitions
The terms used herein have their ordinary meaning and the meaning of such terms is independent at each occurrence thereof. That notwithstanding and except where stated otherwise, the following definitions apply throughout the specification and claims. Chemical names, common names, and chemical structures may be used interchangeably to describe the same structure. If a chemical compound is referred to using both a chemical structure and a chemical name, and an ambiguity exists between the structure and the name, the structure predominates. These definitions apply regardless of whether a term is used by itself or in combination with other terms, unless otherwise indicated. Hence, the definition of “alkyl” applies to “alkyl” as well as the “alkyl” portions of “hydroxyalkyl,” “fluoroalkyl,” “—O-alkyl,” etc.
As used herein, and throughout this disclosure, the following terms, unless otherwise indicated, shall be understood to have the following meanings:
A “patient” is a human or non-human mammal. In one embodiment, a patient is a human. In another embodiment, a patient is a chimpanzee.
The term “therapeutically effective amount” as used herein, refers to an amount of the compound of Formula (I) and/or an additional therapeutic agent, or a composition thereof that is effective in producing the desired therapeutic, ameliorative, inhibitory or preventative effect when administered to a patient suffering from pain or an inflammatory disease or disorder. In the combination therapies of the present invention, a therapeutically effective amount can refer to each individual agent or to the combination as a whole, wherein the amounts of all agents administered are together effective, but wherein the component agent of the combination may not be present individually in an effective amount.
The term “preventing,” as used herein with respect to pain or an inflammatory disease or disorder, refers to reducing the likelihood of pain or an inflammatory disease or disorder.
The term “alkyl,” as used herein, refers to an aliphatic hydrocarbon group having one of its hydrogen atoms replaced with a bond. An alkyl group may be straight or branched and contain from about 1 to about 20 carbon atoms. In one embodiment, an alkyl group contains from about 1 to about 12 carbon atoms. In different embodiments, an alkyl group contains from 1 to 6 carbon atoms (C1-C6 alkyl) or from 1 to 3 carbon atoms (C1-C3 alkyl). Non-limiting examples of alkyl groups include methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl, isobutyl, tert-butyl, n-pentyl, neopentyl, isopentyl, n-hexyl, isohexyl and neohexyl. In one embodiment, an alkyl group is linear. In another embodiment, an alkyl group is branched. Unless otherwise indicated, an alkyl group is unsubstituted.
The term “alkylene,” as used herein, refers to an alkyl group, as defined above, wherein one of the alkyl group's hydrogen atoms has been replaced with a bond. Non-limiting examples of alkylene groups include —CH2—, —CH2CH2—, —CH2CH2CH2—, —CH2CH2CH2CH2—, —CH(CH3)CH2CH2—, —CH(CH3)— and —CH2CH(CH3)CH2—. In one embodiment, an alkylene group has from 1 to about 6 carbon atoms. In another embodiment, an alkylene group has from 1 to 3 carbon atoms. In another embodiment, an alkylene group is branched. In another embodiment, an alkylene group is linear. In one embodiment, an alkylene group is —CH2—. The term “C1-C3 alkylene” refers to an alkylene group having from 1 to 3 carbon atoms. Unless otherwise indicated, an alkylene group is unsubstituted.
The term “alkenyl,” as used herein, refers to an aliphatic hydrocarbon group containing at least one carbon-carbon double bond and having one of its hydrogen atoms replaced with a bond. An alkenyl group may be straight or branched and contain from about 2 to about 15 carbon atoms. In one embodiment, an alkenyl group contains from about 3 to 6 carbon atoms. Non-limiting examples of alkenyl groups include ethenyl, propenyl, n-butenyl, 3-methylbut-2-enyl, n-pentenyl, octenyl and decenyl. The term “C2-C6 alkenyl” refers to an alkenyl group having from 2 to 6 carbon atoms. Unless otherwise indicated, an alkenyl group is unsubstituted.
The term “alkenylene,” as used herein, refers to an alkenyl group, as defined above, wherein one of the alkenyl group's hydrogen atoms has been replaced with a bond. Non-limiting examples of alkenylene groups include —CH2CH═CH—, —CH2CH═CHCH2—, and —CH(CH3)CH═CH—. In one embodiment, an alkenylene group has from 3 to 6 carbon atoms. In another embodiment, an alkenylene group is branched. In another embodiment, an alkenylene group is linear. The term “C3-C6 alkenylene” refers to an alkenylene group having from 3 to 6 carbon atoms. Unless otherwise indicated, an alkenylene group is unsubstituted.
The term “alkynyl,” as used herein, refers to an aliphatic hydrocarbon group containing at least one carbon-carbon triple bond and having one of its hydrogen atoms replaced with a bond. An alkynyl group may be straight or branched and contain from about 2 to about 15 carbon atoms. In one embodiment, an alkynyl group contains from about 3 to about 6 carbon atoms. Non-limiting examples of alkynyl groups include ethynyl, propynyl, 2-butynyl and 3-methylbutynyl. Unless otherwise indicated, an alkynyl group is unsubstituted.
The term “alkynylene,” as used herein, refers to an alkynyl group, as defined above, wherein one of the alkynyl group's hydrogen atoms has been replaced with a bond. Non-limiting examples of alkynylene groups include —CH2C≡C—, —CH2C≡CCH2—, and —CH(CH3)C≡C—. In one embodiment, an alkynylene group has from 3 to 6 carbon atoms. In another embodiment, an alkynylene group is branched. In another embodiment, an alkynylene group is linear. The term “C3-C6 alkynylene” refers to an alkynylene group having from 3 to 6 carbon atoms. Unless otherwise indicated, an alkenylene group is unsubstituted.
The term “alkoxy” as used herein, refers to an —O-alkyl group, wherein an alkyl group is as defined above. Non-limiting examples of alkoxy groups include methoxy, ethoxy, n-propoxy, isopropoxy, n-butoxy and t-butoxy. An alkoxy group is bonded via its oxygen atom.
The term “aryl,” as used herein, refers to an aromatic monocyclic or multicyclic ring system comprising from about 6 to about 14 carbon atoms. In one embodiment, an aryl group contains from about 6 to 10 carbon atoms (C6-C10 aryl). In another embodiment an aryl group is phenyl. An aryl group can be optionally substituted with one or more “ring system substituents” which may be the same or different, and are as defined herein below. In one embodiment, an aryl group can be optionally fused to a cycloalkyl or cycloalkanoyl group. Non-limiting examples of aryl groups include phenyl and naphthyl. Unless otherwise indicated, an aryl group is unsubstituted.
The terms “aza- or diazabicycloheterocycloalkyl” refer to saturated or mono-unsaturated cyclic systems having a first ring which is a 5, 6, or 7-membered ring having one or two nitrogen ring atoms with the remainder of the ring atoms being carbon atoms and a second ring formed from an alkylene bridge having 1 or 2 carbon atoms which is joined to two non-adjacent ring carbon atoms of the first ring. In one embodiment, an aza- or diabicycloheterocycloalkyl is a group selected from 2,5-diazabicyclo[2.2.1]heptane and 2,5-diazabicyclo[2.2.2]octane, 3,8-diazabicyclo[3.2.1]octane, and 8-azabyciyclo[3.2.1]oct-2-ene.
The term “carbamyl,” as used herein, refers to the moiety —C(O)NH2 wherein the point of attachment is through the carbonyl carbon atom.
The term “cycloalkyl,” as used herein, refers to a non-aromatic mono- or multicyclic ring system comprising from about 3 to about 10 ring carbon atoms. In one embodiment, a cycloalkyl contains from about 5 to 10 ring carbon atoms. In another embodiment, a cycloalkyl contains from about 3 to about 7 ring atoms. In another embodiment, a cycloalkyl contains from about 5 to about 6 ring atoms. Non-limiting examples of monocyclic cycloalkyls include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl and cyclooctyl. Non-limiting examples of multicyclic cycloalkyls include 1-decalinyl, norbornyl and adamantyl. The term “C3-C7 cycloalkyl” refers to a cycloalkyl group having from 3 to 7 ring carbon atoms. Unless otherwise indicated, a cycloalkyl group is unsubstituted.
The term “halo,” as used herein, means —F, —Cl, —Br or —I. In one embodiment, a halo group is —F or —Cl. In another embodiment, a halo group is F.
The term “fluoroalkyl,” as used herein, refers to an alkyl group as defined above, wherein one or more of the alkyl group's hydrogen atoms has been replaced with a fluorine. In one embodiment, a fluoroalkyl group has from 1 to 6 carbon atoms. In another embodiment, a fluoroalkyl group is substituted with from 1 to 3 F atoms. Non-limiting examples of fluoroalkyl groups include —CH2F, —CHF2, and —CF3. The term “C1-C3 fluoroalkyl” refers to a fluoroalkyl group having from 1 to 3 carbon atoms.
The term “hydroxyalkyl,” as used herein, refers to an alkyl group as defined above, wherein one of the alkyl group's hydrogen atoms has been replaced with a hydroxyl moiety. In one embodiment, a hydroxyalkyl group has from 1 to 3 carbon atoms. Non-limiting examples of hydroxyalkyl groups include —CH2OH, —CH2CH2OH, and —CH2CH(CH3)CH2OH, and —CH(CH3)CH2OH. The term “C1-C3 hydroxyalkyl” refers to a hydroxyalkyl group having from 1 to 3 carbon atoms.
The term “heteroaryl,” as used herein, refers to an aromatic monocyclic or multicyclic ring system comprising about 5 to about 14 ring atoms, wherein from 1 to 4 of the ring atoms is independently O, N or S and the remaining ring atoms are carbon atoms. In one embodiment, a heteroaryl group has 5 to 10 ring atoms. In another embodiment, a heteroaryl group is monocyclic and has 5 or 6 ring atoms. In another embodiment, a heteroaryl group is bicyclic. A heteroaryl group is joined via a ring carbon atom, and any nitrogen atom of a heteroaryl can be optionally oxidized to the corresponding N-oxide. The term “heteroaryl” also encompasses a heteroaryl group, as defined above, which is fused to a benzene ring. Non-limiting examples of heteroaryls include pyridyl, pyrazinyl, furanyl, thienyl, pyrimidinyl, pyridone (including N-substituted pyridones), isoxazolyl, isothiazolyl, oxazolyl, oxadiazolyl, thiazolyl, pyrazolyl, furazanyl, pyrrolyl, triazolyl, 1,2,4-thiadiazolyl, pyrazinyl, pyridazinyl, quinoxalinyl, phthalazinyl, oxindolyl, imidazo[1,2-a]pyridinyl, imidazo[2,1-b]thiazolyl, benzofurazanyl, indolyl, azaindolyl, benzimidazolyl, benzothienyl, quinolinyl, imidazolyl, benzimidazolyl, thienopyridyl, quinazolinyl, thienopyrimidyl, pyrrolopyridyl, imidazopyridyl, isoquinolinyl, benzoazaindolyl, 1,2,4-triazinyl, benzothiazolyl and the like. In one embodiment, a heteroaryl group is a 5-membered heteroaryl. In another embodiment, a heteroaryl group is a 6-membered heteroaryl. In another embodiment, a heteroaryl group comprises a 5- to 6-membered heteroaryl group fused to a benzene ring. Unless otherwise indicated, a heteroaryl group is unsubstituted.
The term “heterocyclyl,” as used herein, refers to a non-aromatic saturated monocyclic or multicyclic ring system comprising 3 to about 11 ring atoms, wherein from 1 to 4 of the ring atoms are independently O, S, or N, and the remainder of the ring atoms are carbon atoms. A heterocyclyl group can be joined via a ring carbon or ring nitrogen atom. In one embodiment, a heterocyclyl group is monocyclic and has from about 3 to about 7 ring atoms. In another embodiment, a heterocyclyl group is monocyclic has from about 4 to about 7 ring atoms. In another embodiment, a heterocyclyl group is bicyclic and has from about 7 to about 11 ring atoms. In still another embodiment, a heterocyclyl group is monocyclic and has 5 or 6 ring atoms. In one embodiment, a heterocyclyl group is monocyclic. In another embodiment, a heterocyclyl group is bicyclic. The term “heterocyclyl” also encompasses a heterocyclyl group, as defined above, which is fused to an aryl (e.g., benzene) or heteroaryl ring. The nitrogen or sulfur atom of the heterocyclyl can be optionally oxidized to the corresponding N-oxide, S-oxide or S,S-dioxide. Non-limiting examples of monocyclic heterocyclyl rings include oxetanyl, piperidyl, pyrrolidinyl, piperazinyl, morpholinyl, thiomorpholinyl, thiazolidinyl, 1,4-dioxanyl, tetrahydrofuranyl, tetrahydrothiophenyl, delta-lactam, delta-lactone, and the like.
In one embodiment, a heterocyclyl group is a 5- to 6-membered monocyclic heterocyclyl. In another embodiment, a heterocyclyl group is a 5-membered monocyclic heterocyclyl. In another embodiment, a heterocyclyl group is a 6-membered monocyclic heterocyclyl. The term “5- to 6-membered heterocyclyl” refers to a monocyclic heterocyclyl group having from 5 to 6 ring atoms. Unless otherwise indicated, a heterocyclyl group is unsubstituted.
“Heterocyclenyl” means a non-aromatic monocyclic or multicyclic ring system comprising about 3 to about 10 ring atoms, preferably about 5 to about 10 ring atoms, in which one or more of the atoms in the ring system is an element other than carbon, for example nitrogen, oxygen or sulfur atom, alone or in combination, and which contains at least one carbon-carbon double bond or carbon-nitrogen double bond. In specific embodiments of the ring system, from 1 to 4 of the ring atoms are independently O, S, or N, and the remainder of the ring atoms are carbon atoms. There are no adjacent oxygen and/or sulfur atoms present in the ring system. Preferred heterocyclenyl rings contain about 5 to about 6 ring atoms. The prefix aza, oxa or thia before the heterocyclenyl root name means that at least a nitrogen, oxygen or sulfur atom respectively is present as a ring atom. Unless otherwise indicated, a heterocyclenyl group is unsubstituted. The nitrogen or sulfur atom of the heterocyclenyl can be optionally oxidized to the corresponding N-oxide, S-oxide or S,S-dioxide. Non-limiting examples of suitable heterocyclenyl groups include 1,2,3,4-tetrahydropyridinyl, 1,2-dihydropyridinyl, 1,4-dihydropyridinyl, 1,2,3,6-tetrahydropyridinyl, 1,4,5,6-tetrahydropyrimidinyl, 2-pyrrolinyl, 3-pyrrolinyl, 2-imidazolinyl, 2-pyrazolinyl, dihydroimidazolyl, dihydrooxazolyl, dihydrooxadiazolyl, dihydrothiazolyl, 3,4-dihydro-2H-pyranyl, dihydrofuranyl, fluorodihydrofuranyl, 7-oxabicyclo[2.2.1]heptenyl, dihydrothiophenyl, dihydrothiopyranyl, and the like.
The term “substituted” means that one or more hydrogens on the atoms of the designated are replaced with a selection from the indicated group, provided that the atoms' normal valencies under the existing circumstances are not exceeded, and that the substitution results in a stable compound. Combinations of substituents and/or variables are permissible only if such combinations result in stable compounds. By “stable compound’ or “stable structure” is meant a compound that is sufficiently robust to survive isolation to a useful degree of purity from a reaction mixture, and formulation into an efficacious therapeutic agent.
When any substituent or variable occurs more than one time in any constituent or the compound of Formula (I), its definition on each occurrence is independent of its definition at every other occurrence, unless otherwise indicated.
The term “in purified form,” as used herein, refers to the physical state of a compound after the compound is isolated from a synthetic process (e.g., from a reaction mixture), a natural source, or a combination thereof. The term “in purified form,” also refers to the physical state of a compound after the compound is obtained from a purification process or processes described herein or well-known to the skilled artisan (e.g., chromatography, recrystallization and the like), in sufficient purity to be characterizable by standard analytical techniques described herein or well-known to the skilled artisan.
It should also be noted that any carbon as well as heteroatom with unsatisfied valences in the text, schemes, examples and tables herein is assumed to have the sufficient number of hydrogen atom(s) to satisfy the valences.
One or more compounds of the invention may exist in unsolvated as well as solvated forms with pharmaceutically acceptable solvents such as water, ethanol, and the like, and it is intended that the invention embrace both solvated and unsolvated forms. “Solvate” means a physical association of a compound of this invention with one or more solvent molecules. This physical association involves varying degrees of ionic and covalent bonding, including hydrogen bonding. In certain instances the solvate will be capable of isolation, for example when one or more solvent molecules are incorporated in the crystal lattice of the crystalline solid. “Solvate” encompasses both solution-phase and isolatable solvates. Non-limiting examples of suitable solvates include ethanolates, methanolates, and the like. “Hydrate” is a solvate wherein the solvent molecule is H2O.
The compounds of Formula (I) may contain asymmetric or chiral centers, and, therefore, exist in different stereoisomeric forms. It is intended that all stereoisomeric forms of the compounds of Formula (I) as well as mixtures thereof, including racemic mixtures, form part of the present invention.
Diastereomeric mixtures can be separated into their individual diastereomers on the basis of their physical chemical differences by methods well known to those skilled in the art, such as, for example, by chromatography and/or fractional crystallization. Enantiomers can be separated by converting the enantiomeric mixture into a diastereomeric mixture by reaction with an appropriate optically active compound (e.g., chiral auxiliary such as a chiral alcohol or Mosher's acid chloride), separating the diastereomers and converting (e.g., hydrolyzing) the individual diastereomers to the corresponding pure enantiomers. Also, some of the compounds of Formula (I) may be atropisomers (e.g., substituted biaryls) and are considered as part of this invention. Enantiomers can also be separated by use of chiral HPLC column.
It is also possible that the compounds of Formula (I) may exist in different tautomeric forms, and all such forms are embraced within the scope of the invention.
All stereoisomers (for example, geometric isomers, optical isomers and the like) of the present compounds (including those of the salts and solvates of the compounds as well as the salts, solvates and esters of the prodrugs), such as those which may exist due to asymmetric carbons on various substituents, including enantiomeric forms (which may exist even in the absence of asymmetric carbons), rotameric forms, atropisomers, and diastereomeric forms, are contemplated within the scope of this invention. Individual stereoisomers of the compounds of the invention may, for example, be substantially free of other isomers, or may be admixed, for example, as racemates or with all other, or other selected, stereoisomers. The chiral centers of the present invention can have the S or R configuration as defined by the IUPAC 1974 Recommendations.
The compounds of Formula (I) can form salts which are also within the scope of this invention. Reference to a compound of Formula (I) herein is understood to include reference to salts thereof, unless otherwise indicated. The term “salt(s)”, as employed herein, denotes acidic salts formed with inorganic and/or organic acids, as well as basic salts formed with inorganic and/or organic bases. In addition, when a compound of Formula (I) contains both a basic moiety, such as, but not limited to a pyridine or imidazole, and an acidic moiety, such as, but not limited to a carboxylic acid, zwitterions (“inner salts”) may be formed and are included within the term “salt(s)” as used herein. Such acidic and basic salts used within the scope of the invention are pharmaceutically acceptable (i.e., non-toxic, physiologically acceptable) salts. Salts of the compounds of Formula (I) may be formed, for example, by reacting a compound of Formula (I) with an amount of acid or base, such as an equivalent amount, in a medium such as one in which the salt precipitates or in an aqueous medium followed by lyophilization.
Exemplary acid addition salts include acetates, ascorbates, benzoates, benzenesulfonates, bisulfates, borates, butyrates, citrates, camphorates, camphorsulfonates, fumarates, hydrochlorides, hydrobromides, hydroiodides, lactates, maleates, methanesulfonates, naphthalenesulfonates, nitrates, oxalates, phosphates, propionates, salicylates, succinates, sulfates, tartarates, thiocyanates, toluenesulfonates (also known as tosylates,) and the like. Additionally, acids which are generally considered suitable for the formation of pharmaceutically useful salts from basic pharmaceutical compounds are discussed, for example, by P. Stahl et al, Camille G. (eds.) Handbook of Pharmaceutical Salts. Properties, Selection and Use. (2002) Zurich: Wiley-VCH; S. Berge et al, Journal of Pharmaceutical Sciences (1977) 66(1) 1-19; P. Gould, International J. of Pharmaceutics (1986) 33 201-217; Anderson et al, The Practice of Medicinal Chemistry (1996), Academic Press, New York; and in The Orange Book (Food & Drug Administration, Washington, D.C. on their website). These disclosures are incorporated herein by reference.
Exemplary basic salts include ammonium salts, alkali metal salts such as sodium, lithium, and potassium salts, alkaline earth metal salts such as calcium and magnesium salts, salts with organic bases (for example, organic amines) such as dicyclohexylamines, t-butyl amines, and salts with amino acids such as arginine, lysine and the like. Basic nitrogen-containing groups may be quarternized with agents such as lower alkyl halides (e.g., methyl, ethyl, and butyl chlorides, bromides and iodides), dialkyl sulfates (e.g., dimethyl, diethyl, and dibutyl sulfates), long chain halides (e.g., decyl, lauryl, and stearyl chlorides, bromides and iodides), aralkyl halides (e.g., benzyl and phenethyl bromides), and others.
The present invention further includes the compounds of Formula (I) in all their isolated forms. For example, the above-identified compounds are intended to encompass all forms of the compounds such as, any solvates, hydrates, stereoisomers, and tautomers thereof.
As used herein, the term “composition” is intended to encompass a product comprising the specified ingredients in the specified amounts, as well as any product which results, directly or indirectly, from combination of the specified ingredients in the specified amounts.
In the compounds of generic Formula (I), the atoms may exhibit their natural isotopic, abundances, or one or more of the atoms may be artificially enriched in a particular isotope having the same atomic number, but an atomic mass or mass number different from the atomic mass or mass number predominantly found in nature. The present invention is meant to include all suitable isotopic variations of the compounds of generic Formula (I). For example, different isotopic forms of hydrogen (H) include protium (1H) and deuterium (2H). Protium is the predominant hydrogen isotope found in nature. Enriching for deuterium may afford certain therapeutic advantages, such as increasing in vivo half-life or reducing dosage requirements, or may provide a compound useful as a standard for characterization of biological samples. Isotopically-enriched compounds within generic Formula (I) can be prepared without undue experimentation by conventional techniques well known to those skilled in the art or by processes analogous to those described in the Schemes and Examples herein using appropriate isotopically-enriched reagents and/or intermediates.
Compounds of the Invention
The present invention provides compound of Formula (I) or pharmaceutically acceptable salts thereof, wherein J1, J2, R2, R2, R3, R22, Ra, Rb, Rc, Rd, X, Y, b, n, and q are as defined above for the compound of Formula (I). The compounds of Formulas (IA), (IB), and (IC) as are described in detail below, are embodiments of the compound of Formula (I). The structural formula illustrated below indicates the peripheral numbering of the ring system.




In specific embodiments of the compound of Formula (I), wherein R1 is —C(O)—N(R6a)(R6b) or —S(O)2—N(R6)(R6b); and R6a and R6b together with the N atom to which they are attached form R6H, it is be understood that two R9 moieties can be geminally substituted on a common ring carbon of R6H form RYC, such that R1 forms the group:




wherein R6H and RYC are as described above, and R11 is either absent or present.

In certain embodiments of the compound of the Formula (I), wherein Y is




it is to be understood that the bonds joining the illustrated cycloalkyl ring to the chain can either be attached on different ring carbon atoms, e.g., on vicinal ring carbon atoms, or on the same ring carbon atom. For example, in some embodiments, the group





In embodiment no. 1 of the compound of Formula (I), R1 is substituted on the 6 or 7 position of the illustrated bicyclic ring of Formula 1.
In embodiment no. 2, R1 is substituted on the 7 or 8 position of the illustrated bicyclic ring of Formula (I), and J2 is C(H), C(R1), or C(R22). In other words, R1 is substituted on a ring carbon atom that is beta to the ring fusion of the illustrated bicyclic ring as shown below.




In embodiment no. 3, the compound has the formula (IA)




wherein J1 and J2 are independently C(H) or C(R22), or N wherein the following provisos apply:

(i) no more than one of J1 and J2 is N, and
(ii) no more than one of J1 and J2 is C(R22).
In embodiment no. 4, R1 is selected from the group consisting of:
(i) —C(O)—N(R6a)(R6B), and
(ii) —S(O)2—N(R6a)(R6b).
In embodiment no. 5, R1 is as described above in embodiment no. 4, and
(i) R6a is H and

    
    
        R6b is -Q-RAH or -Q-RHC; or
    
    


(ii) R6a and R6b together with the N atom to which they are attached form R6H.
In embodiment no. 6, R1 is —C(O)—N(R6a)(R6b)
In embodiment no. 7, R1 is —C(O)—N(R6a)(R6b), wherein R6a is H, and R6b is -Q-RAH or -Q-RHC. In specific instances of embodiment no. 7, R6b is -Q-RHC, wherein Q is absent and RHC is




wherein v is 1 or 2, and R12 is present or absent.

In embodiment no. 8, R1 is
—C(O)—N(R6a)(R6b), wherein R6a and R6b together with the N atom to which they are attached form R6H. In specific instances of embodiment no. 8, R6H is




wherein u is 1, 2, or 3, and R9 is present or absent.

In embodiment no. 9 of the compound of Formula (I), R1 is —C(O)C(R7a)(R7b)(R7c). In specific instances of embodiment no. 9, R1 is —C(O)C(H)(R7a)(R7b), wherein R7a and R7b together with the carbon atom to which they are attached form RPC, wherein RPC is C3-C7 cycloalkyl. For example, in one instance of embodiment no. 9, R1 is




In embodiment no. 10 of the compound of Formula (I), R1 is —C(O)—O—C(R7a)(R7b)(R7c). For example, in one instance of embodiment no. 10, R1 is —C(O)—O—CH3.
In embodiment no. 11, Y is selected from the group consisting of

    
    
        (i) a bond,
    
    






In embodiment no. 12, the group




is selected from the group consisting of:





In embodiment no. 13, the compound has the Formula (IA) as described above in embodiment no. 3; and the group




is as described above in embodiment no. 12.

In embodiment no. 14, R2 is phenyl, pyridyl, or thienyl; wherein R2 is unsubstituted or substituted by 1 to 2 R5 groups independently selected from the group consisting of fluoro, chloro, trifluoromethyl, C1-C3 alkoxy, —CN, and —OCF3;
In embodiment no. 15, b is 0, such that R22 is absent.
In embodiment no. 16, the compound has the Formula (IA) as described above in embodiment no. 3, R1 is as described above in embodiment no. 4, the group




is as described above in embodiment no. 12, R2 is as described in embodiment no. 14, and b is 0.

In embodiment no. 17, the compound has the Formula (IA) as described above in embodiment no. 3, R1 is as described above in embodiment no. 5, the group




is as described above in embodiment no. 12, R2 is as described in embodiment no. 14, and b is 0.

In embodiment no. 18, the compound of the Formula (I) has the Formula (IB)




wherein

J1 is C(H) or N;

R1 is —C(O)—N(R6a)(R6b);

I) R6a is H and R6b is:

    
    
        a) -Q-RAH, wherein RAH is phenyl or pyridyl,
        
            and wherein RAH is unsubstituted or substituted with 1 to 2 R8 moieties independently selected from the group consisting of halo, C1-C3 alkyl, C1-C3 alkoxy, trifluoromethyl, trifluoromethoxy, and —CN;
            Q is selected from the group consisting of:
            
                (i) a bond;
                (ii)
            
            
        
        
    
    






    
    
        
        
            
            
                 wherein Re and Rf are independently H, C1-C3 alkyl, or trifluoromethyl;
                (iii) C2-C4 alkylene, wherein said C2-C6 alkylene is unsubstituted;
            
            
            b) -Q-RHC, wherein RHC is
            
                (i) 5- to 6-membered heterocyclyl containing 1 heteroatom selected from the group consisting of N and O; or
                (ii) C5-C7 cycloalkyl, wherein said C5-C7 cycloalkyl is optionally fused to a benzene ring;
                and wherein RHC is unsubstituted or substituted with 1 to 2 R12 moieties independently selected from the group consisting of C1-C3 alkyl, halo, and hydroxyl, or wherein when two R12 moieties are geminally substituted on the same carbon atom, the two geminally substituted R12 moieties, together with the carbon atom on which they are attached form —C(O)—;
            
            
        
        
    
    


II) or R6a and R6b together with the N atom to which they are attached form R6H, wherein

    
    
        R6H is independently selected from the group consisting of:
        
            a) a 4- to 6-membered heterocyclyl, optionally containing one additional nitrogen atom, wherein said 4- to 6-membered heterocyclyl is optionally fused to phenyl; and
            b) a 5- to 6-membered heterocyclenyl, optionally containing one additional nitrogen atom, wherein said 4- to 6-membered heterocyclyl is optionally fused to phenyl;
            
                wherein R6H is unsubstituted or substituted by 1 to 2 R9 moieties wherein each R9 moiety is independently C1-C3 alkyl, F, Cl, —CN, or
                
                    R9 is —Z—RCY wherein
                     Z is a bond or —CH2—;
                     RCY is selected from the group consisting of:
                     (i) phenyl;
                     (ii) 5- to 6-membered heteroaryl containing 1 to 3 N atoms; or
                     (iii) 5- to 6-membered heterocyclyl containing 2 N atoms, wherein said 5- to 6-membered heterocyclyl of RCY is fused to phenyl;
                     wherein RCY is unsubstituted or substituted by 1 to 2 R10 moieties;
                     each R10 moiety is independently C1-C3 alkyl, halo, C1-C3 alkoxy, —(C1-C3 alkylene)-(C1-C3 alkoxy), or —CN, or, wherein two R10 moieties are geminally substituted on a common carbon atom, together with the carbon atom on which they are substituted, form —C(O)—;
                    or, optionally, where two R9 moieties are geminally substituted on a common ring carbon of R6H, the two R9 moieties, together with the ring carbon on which they are substituted, form RYC, wherein RYC is
                     (i) a 5- to 6-membered cycloalkyl, wherein said or 5- to 6-membered cycloalkyl is fused to phenyl; or
                     (ii) a 4- to 6-membered heterocyclyl containing 1 to 2 N atoms or 1 O atom, wherein said or 4- to 6-membered heterocyclyl is optionally fused to phenyl;
                     wherein RYC is unsubstituted or substituted by 1 to 3 R11 moieties;
                     each R11 moiety is independently C1-C3 alkyl,
                     —C(O)—(C1-C3 alkyl), or phenyl, or, wherein two R11 moieties are geminally substituted on a common carbon atom, together with the carbon atom on which they are substituted, form —C(O)—;

Y is selected from the group consisting of

                
                
            
            
        
        
    
    


(i) a bond, (ii)




Rc is H or methyl;

Rd is H;

R2 is phenyl, pyridyl, or thienyl;

    
    
        wherein R2 is unsubstituted or substituted by 1 to 2 R5 groups independently selected from the group consisting of fluoro, chloro, trifluoromethyl, C1-C3 alkoxy, —CN, and —OCF3;

q is 0, 1, or 2.

    
    


In embodiment no. 19, J1 is CH, and the structural formula and the remaining variables are as described above in embodiment no. 18.
In embodiment no. 20, R2 is unsubstituted or substituted phenyl, and the structural formula and the remaining variables are as described above in embodiment no. 18.
In embodiment no. 21, the group




and the structural formula and the remaining variables are as described above in embodiment no. 18.

In embodiment no. 22, the compound of the Formula (I) has the Formula (IB)
J1 is C(H);
R1 is —C(O)—N(R6a)(R6b);
I) R6a is H and R6b) is:

    
    
        a) -Q-RAH, wherein RAH is phenyl,
        
            and wherein RAH is unsubstituted or substituted with 1 R8 moiety selected from the group consisting of fluoro and chloro;
            Q is selected from the group consisting of:
            
                (i) a bond;
                (ii)
            
            
        
        
    
    






wherein Re is H, and Rf is H or methyl;

    
    
        b) -Q-RHC, wherein RHC is C5-C6 cycloalkyl, wherein said C5-C6 cycloalkyl is fused to a benzene ring;
        
            and wherein RHC is unsubstituted or substituted with 1 to 2 R12 moieties independently selected from the group consisting of fluoro and chloro;
        
        
    
    


II) or R6a and R6b together with the N atom to which they are attached form R6H, wherein

    
    
        R6H is azetidinyl, pyrrolidinyl, piperidinyl, or piperazinyl:
        
            wherein R6H is substituted by —Z—RCY;
            
                wherein
                
                    Z is a bond;
                    RCY is unsubstituted phenyl or phenyl substituted by 1 to 2 R10 moieties selected from the group consisting of fluoro and chloro; the group
                
                
            
            
        
        
    
    






R2 is unsubstituted phenyl or phenyl substituted by 1 to 2 fluoro or chloro.

In embodiment no. 23, the compound of the Formula (I) has the Formula (IC)




wherein

R6a is —C(O)—N(R6a)(R6b);

I) R6a is H and R6b is:

    
    
        a) -Q-RAH, wherein RAH is phenyl,
        
            and wherein RAH is unsubstituted or substituted with 1 R8 moiety selected from the group consisting of halo and —CN;
            Q is selected from the group consisting of
            
                (i) a bond;
                (ii)
            
            
        
        
    
    






wherein Re is H, and Rf is H or methyl;

    
    
        b) -Q-RHC, wherein RHC is C5-C6 cycloalkyl, wherein said C5-C6 cycloalkyl is fused to a benzene ring;
        
            and wherein RHC is unsubstituted or substituted with 1 to 2 R12 moieties independently selected from the group consisting of halo and —CN;
        
        
    
    


II) or R6a and R6b together with the N atom to which they are attached form R6H, wherein

    
    
        R6H is azetidinyl, pyrrolidinyl, piperidinyl, or piperazinyl;
        
            wherein R6H is substituted with —Z—RCY wherein
            
                Z is a bond; and
                RCY is unsubstituted phenyl or phenyl substituted by 1 to 2 R10 moieties selected from the group consisting of halo and —CN;
            
            
            R6H is optionally substituted by 1 to 2 R9 moieties wherein each R9 moiety is independently C1-C3 alkyl, halo or —CN, and

R2 is unsubstituted or substituted by 1 to 2 R5 groups independently selected from the group consisting of fluoro, chloro, C1-C3 alkyl, trifluoromethyl, C1-C3 alkoxy, —CN, and —OCF3.

        
        
    
    


In embodiment no. 24, the compound of the Formula (I) has the Formula (IC) wherein
R1 is —C(O)—N(R6a)(R6b);
I) R6a is H and R6b is:

    
    
        a) -Q-RAH, wherein RAH is phenyl,
        
            and wherein RAH is unsubstituted or substituted with 1 R8 moiety selected from the group consisting of fluoro and —CN;
        
        
        b) -Q-RHC, wherein RHC is C5-C6 cycloalkyl, wherein said C5-C6 cycloalkyl is fused to a benzene ring;
        
            and wherein RHC is unsubstituted or substituted with 1 to 2 R12 moieties independently selected from the group consisting of halo and —CN;
        
        
    
    


II) or R6a and R6b together with the N atom to which they are attached form R6H, wherein

    
    
        R6H is pyrrolidinyl, piperidinyl, or piperazinyl;
        
            wherein R6H is substituted with —Z—RCY wherein
            
                RCY is unsubstituted phenyl or phenyl substituted by 1 to 2 R10 moieties selected from the group consisting of halo and —CN;
            
            
            R6H is optionally substituted by 1 to 2 R9 moieties wherein each R9 moiety is independently C1-C3 alkyl, halo or —CN,

R2 is unsubstituted phenyl; and

Q and Z are as described above in embodiment no. 23.

        
        
    
    


In embodiment no. 25, R6a and R6b together with the N atom to which they are attached form R6H, wherein R6H is piperazinyl, and the structural formula and the remaining variables are as described above in embodiment no. 24.
The invention also provides any one of the compounds specified in Tables A and B in the Examples section below, which tables include compounds 3, 3T, 3U, 3V, 3W, 3X, 3Y, 3Z, 3AA, 3AB, 3AC, 3AD, 3AE, 3AF, 3AG, 4, 4D, 4E, 4F, 4G, 5, 5G, 5N, 5o, 5P, 5Q, 5R, 5S, 6, 6F, 6G, 7, 7C, 8, 8G, 9, 10, 10C, 10D, 10E, 10F, 11, 11D, 11E, 11F, 12, 13, 14, 14C, 14D, 14E, 14F, 14G, 14H, 15A, 15B, 15C, 15D, 15E, 15F, 15G, 15H, 15i, 15J, 15K, 15L, 15M, 15N, 15o, 15P, 15Q, 15R, 15S, 15T, 15U, 15V, 15X, 15Y, 15Z, 15AA, 15AB, 15AC, 15AD, 15AE, 15AF, 15AG, 15AH, 15Ai, 15AJ, 15AK, 15AL, 15AM, 15AN, 15Ao, 15AP, 15AQ, 15AR, 15AS, 15AT, 15AU, 15AV, 15AW, 15AX, 15AY, 15AZ, 15BA, 15BB, 15BC, 15BD, 15BE, 15BF, 15BG, 15BH, 15Bi, 15BJ, 15BK, 15BL, 15BM, 15BN, 15Bo, 15BQ, 15BR, 15BS, 15BT, 15BU, 16, 16F, 16G, 16H, 16i, 16J, 16K, 16L, 16M, 16N, 16o, 16P, 16Q, 16R, 16S, 16T, 16U, 16V, 16W, 16X, 16Y, 16Z, 16AA, 16AB, 16AC, 16AD, 16AE, 16AF, 16AG, 16AH, 16Ai, 16AJ, 16AK, 16AL, 16AM, 16AN, 16Ao, 16AP, 16AQ, 16AR, 16AS, 17, 17D, 17E, 17F, 17G, 17H, 17i, 17J, 17K, 17L, 17M, 17N, 17o, 17P, 17Q, 17R, 17S, 17T, 17U, 17V, 17V, 17W, 17X, 17Y, 17Z, 18, 18D, 18E, 18F, 18G, 18H, 19, 19F, 19G, 19H, 19i, 19J, 19K, 20, 20F, 20G, 20H, 21, 22, 22D, 23, 24, 24H, 24i, 25, 26, 26E, 26F, 26G, 26H, 26i, 26J, 26K, 26L, 26M, 26N, 26o, 26P, 26Q, 26R, 26S, 26T, 26U, 26V, 26W, 26X, 26Y, 26Z, 26AA, 26AB, 28, 29, 30, 30E, 31, 31C, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 44, 44D, 44E, 44F, 44G, 44H, 44i, 44J, 44K, 44L, 44M, 44N, and 100-728, or a pharmaceutically acceptable salt thereof. The structural formulas and names of these compounds are as set forth in the Examples section below.
In another embodiment, the invention also provides any one of the compounds specified in Table A in the Examples section below, which table includes compounds 3, 3T, 3U, 3V, 3W, 3X, 3Y, 3Z, 3AA, 3AB, 3AC, 3AD, 3AE, 3AF, 3AG, 4, 4D, 4E, 4F, 4G, 5, 5G, 5N, 5o, 5P, 5Q, 5R, 5S, 6, 6F, 6G, 7, 7C, 8, 8G, 9, 10, 10C, 10D, 10E, 10F, 11, 11D, 11E, 11F, 12, 13, 14, 14C, 14D, 14E, 14F, 14G, 14H, 15A, 15B, 15C, 15D, 15E, 15F, 15G, 15H, 15i, 15J, 15K, 15L, 15M, 15N, 15o, 15P, 15Q, 15R, 15S, 15T, 15U, 15V, 15X, 15Y, 15Z, 15AA, 15AB, 15AC, 15AD, 15AE, 15AF, 15AG, 15AH, 15Ai, 15AJ, 15AK, 15AL, 15AM, 15AN, 15Ao, 15AP, 15AQ, 15AR, 15AS, 15AT, 15AU, 15AV, 15AW, 15AX, 15AY, 15AZ, 15BA, 15BB, 15BC, 15BD, 15BE, 15BF, 15BG, 15BH, 15Bi, 15BJ, 15BK, 15BL, 15BM, 15BN, 15Bo, 15BQ, 15BR, 15BS, 15BT, 15BU, 16, 16F, 16G, 16H, 16i, 16J, 16K, 16L, 16M, 16N, 16o, 16P, 16Q, 16R, 16S, 16T, 16U, 16V, 16W, 16X, 16Y, 16Z, 16AA, 16AB, 16AC, 16AD, 16AE, 16AF, 16AG, 16AH, 16Ai, 16AJ, 16AK, 16AL, 16AM, 16AN, 16Ao, 16AP, 16AQ, 16AR, 16AS, 17, 17D, 17E, 17F, 17G, 17H, 17i, 17J, 17K, 17L, 17M, 17N, 17o, 17P, 17Q, 17R, 17S, 17T, 17U, 17V, 17V, 17W, 17X, 17Y, 17Z, 18, 18D, 18E, 18F, 18G, 18H, 19, 19F, 19G, 19H, 19i, 19J, 19K, 20, 20F, 20G, 20H, 21, 22, 22D, 23, 24, 24H, 24i, 25, 26, 26E, 26F, 26G, 26H, 26i, 26J, 26K, 26L, 26M, 26N, 26o, 26P, 26Q, 26R, 26S, 26T, 26U, 26V, 26W, 26X, 26Y, 26Z, 26AA, 26AB, 28, 29, 30, 30E, 31, 31C, and 100-253, or a pharmaceutically acceptable salt thereof.
In another specific embodiment, the invention provides any one of the compounds selected from the group consisting of 14, 14D, 14G, 15A, 15B, 15C, 15D, 15E, 15K, 15N, 15P, 15Q, 15R, 15S, 15T, 15X, 15Z, 15AB, 15AC, 15AD, 15AF, 15Ai, 15AJ, 15AK, 15AL, 15AN, 15Ao, 15AP, 15AR, 15AU, 15AV, 15AW, 15AX, 15AY, 15AZ, 15BA, 15BB, 15BC, 15BD, 15BF, 15BH, 15BK, 15BM, 15BN, 15BQ, 15BR, 15BS, 15BT, 15BU, 16, 16F, 16H, 16i, 16J, 16K, 16L, 16N, 16P, 16R, 16S, 16T, 16U, 16V, 16Y, 16AB, 16AC, 16AE, 16AG, 16AH, 16AK, 16AQ, 17, 17D, 17M, 17S, 18, 19, 19G, 20, 24i, 26E, 26J, 26K, 26L, 26N, 26o, 26P, 26S, 26Y, 39, 40, 41, 44H, 44i, 100, 102-107, 109-113, 117, 119-132, 135, 136, 142, 143, 145, 147, 148, 162, 163, 164, 166, 167, 172, 174, 217, 231, 251, 254, 255, 256, 375, 412, 426, 457, 491, 507, 512, 585, and 628 or a pharmaceutically acceptable salt thereof.
In another specific embodiment, the invention provides any one of the compounds selected from the group consisting of 14, 14D, 14G, 15A, 15B, 15C, 15D, 15E, 15K, 15N, 15P, 15Q, 15R, 15S, 15T, 15X, 15Z, 15AB, 15AC, 15AD, 15AF, 15Ai, 15AJ, 15AK, 15AL, 15AN, 15Ao, 15AP, 15AR, 15AU, 15AV, 15AW, 15AX, 15AY, 15AZ, 15BA, 15BB, 15BC, 15BD, 15BF, 15BH, 15BK, 15BM, 15BN, 15BQ, 15BR, 15BS, 15BT, 15BU, 16, 16F, 16H, 16i, 16J, 16K, 16L, 16N, 16P, 16R, 16S, 16T, 16U, 16V, 16Y, 16AB, 16AC, 16AE, 16AG, 16AH, 16AK, 16AQ, 17, 17D, 17M, 17S, 18, 19, 19G, 20, 24i, 26E, 26J, 26K, 26L, 26N, 26o, 26P, 26S, 26Y, 100, 102-107, 109-113, 117, 119-132, 135, 136, 142, 143, 145, 147, 148, 162, 163, 164, 166, 167, 172, 174, 217, 231, 251, 254, and 255, or a pharmaceutically acceptable salt thereof.
The invention also provides a compound of Formula (I) or a pharmaceutically acceptable salt thereof in purified form.
Compositions and Administration
This invention is also directed to pharmaceutical compositions which comprise a compound of Formula (I), or a pharmaceutically acceptable salt of said compound and a pharmaceutically acceptable carrier.
A preferred dosage is about 0.001 to 100 mg/kg of body weight/day of the compound of Formula (I). An especially preferred dosage is about 0.01 to 10 mg/kg of body weight/day of a compound of Formula (I), or a pharmaceutically acceptable salt of said compound.
The term “pharmaceutical composition” is also intended to encompass both the bulk composition and individual dosage units comprised of more than one (e.g., two) pharmaceutically active agents such as, for example, a compound of the present invention and an additional therapeutic agent selected from the lists of the additional agents described herein below, along with any pharmaceutically inactive excipients. The bulk composition and each individual dosage unit can contain fixed amounts of the afore-said “more than one pharmaceutically active agents”. The bulk composition is material that has not yet been formed into individual dosage units. An illustrative dosage unit is an oral dosage unit such as a tablet, a pill and the like. Similarly, the herein-described method of treating a patient by administering a pharmaceutical composition of the present invention is also intended to encompass the administration of the afore-said bulk composition and individual dosage units.
For preparing pharmaceutical compositions from the compounds described by this invention, inert, pharmaceutically acceptable carriers can be either solid or liquid. Solid form preparations include powders, tablets, dispersible granules, capsules, cachets and suppositories. The powders and tablets may be comprised of from about 5 to about 95 percent active ingredient. Suitable solid carriers are known in the art, e.g., magnesium carbonate, magnesium stearate, talc, sugar or lactose. Tablets, powders, cachets and capsules can be used as solid dosage forms suitable for oral administration. Examples of pharmaceutically acceptable carriers and methods of manufacture for various compositions may be found in A. Gennaro (ed.), Remington's Pharmaceutical Sciences, 18th Edition, (1990), Mack Publishing Co., Easton, Pa.
Liquid form preparations include solutions, suspensions and emulsions. Examples of materials useful for forming such liquid form preparations include water or water-propylene glycol solutions for parenteral injection, or sweeteners and opacifiers for oral solutions, suspensions and emulsions. Liquid form preparations may also include solutions or suspensions for intranasal administration.
Aerosol preparations suitable for inhalation may include solutions and solids in powder form, which may be in combination with a pharmaceutically acceptable carrier, such as an inert compressed gas, e.g., nitrogen.
Also included are solid form preparations that are intended to be converted, shortly before use, to liquid form preparations for either oral or parenteral administration. Such liquid forms include solutions, suspensions and emulsions.
The compounds of the invention can also be deliverable transdermally. The transdermal compositions can take the form of creams, lotions, aerosols and/or emulsions and can be included in a transdermal patch of the matrix or reservoir type as are conventional in the art for this purpose.
The compounds of this invention may also be delivered subcutaneously.
Preferably the compound is administered orally.
Preferably, the pharmaceutical preparation is in a unit dosage form. In such form, the preparation is subdivided into suitably sized unit doses containing appropriate quantities of the active component, e.g., an effective amount to achieve the desired purpose.
The quantity of active compound in a unit dose of preparation may be varied or adjusted from about 0.001 mg to about 100 mg per kg body weight of a mammal, preferably from about 0.01 mg to about 10 mg per kg. The actual dosage employed may be varied depending upon the requirements of the patient and the severity of the condition being treated. Determination of the proper dosage regimen for a particular situation is within the skill of the art. For convenience, the total daily dosage may be divided and administered in portions during the day as required.
The compositions of the invention can further comprise one or more additional therapeutic agents, as discussed in further detail below. Accordingly, in one embodiment, the present invention provides compositions comprising: (i) a compound of Formula (I) or a pharmaceutically acceptable salt thereof; (ii) one or more additional therapeutic agents, that are not compounds of Formula (I); and (iii) a pharmaceutically acceptable carrier, wherein the amounts in the composition are together effective to treat one of the disease or conditions discussed above.
Uses of the Compounds
The compounds of Formula (I) bind to CRTH2 and, therefore, are useful in characterizing tissues containing CRTH2, and in identifying further compounds which bind to CRTH2. The general value of the compounds of the invention in binding the CRTH2 receptor can be determined, for example, using the radioligand binding assay described below in the Examples section.
The compounds of Formula (I) can also be useful as modulators of CRTH2 receptor function. In some embodiments, compounds of Formula (I) are antagonists of the CRTH2 receptor. The general value of the compounds of the invention in antagonizing CRTH2 receptor function can be determined, for example, using the chemiluminescent-based cAMP assay, the β-Arrestin assay, or the eosinophil shape change assay described below in the Examples section.
While not being bound by any specific theory, Applicants believe that the compounds of Formula (I) are useful in treating the symptoms of diseases or conditions associated with uncontrolled or inappropriate stimulation of CRTH2 function because of their ability to antagonize the CRTH2 receptor. Accordingly, in one embodiment, the invention provides a method for treating a disease or conditions associated with uncontrolled or inappropriate stimulation of CRTH2 function, comprising administering a therapeutically effective amount of a compound of Formula (I) to a patient in need of such treatment. In certain embodiments, the compound of Formula (I) used in the method is selected from one of the representative compounds listed in Table A as set forth in the Examples section.
Diseases or conditions associated with uncontrolled or inappropriate stimulation of CRTH2 function include (but not limited to) asthma, congestion, allergic rhinitis, atopic dermatitis, chronic obstructive pulmonary disease (“COPD”), dermatitis, inflammatory bowel disease, rheumatoid arthritis, allergic nephritis, conjunctivitis, bronchial asthma, fold allergy, systemic mast cell disorder, anaphylactic shock, urticaria, eczema, itching, inflammation, ischemia-reperfusion injury, cerebrovascular disorders, pleuritis, ulcerative colitis, eosinophil-related diseases, such as Churg-Strauss syndrome and sinusitis, and basophile-related diseases, such as basophilic leukemia and basophilic leukocytosis, in humans and other mammals. Examples of cerebrovascular disorders include stroke.
In certain embodiments, the present invention provides a method for treating asthma, congestion, allergic rhinitis or COPD which comprises administering a therapeutically effective dose of a compound of Formula (I) or a pharmaceutically acceptable salt thereof to a patient in need of such treatment. In a specific embodiment, the disease or condition being treated is asthma. In another embodiment, the disease or condition being treated is COPD.
In addition, compounds of the Formula (I) which act as CRTH2 receptor antagonists can inhibit prostanoid-induced smooth muscle contraction by antagonizing contractile prostanoids or mimicking relaxing prostanoids and hence may be used in the treatment of dysmenorrhea, premature labor and eosinophil related disorders.
In another embodiment, the present invention provides a compound of Formula (I) or a pharmaceutically acceptable salt thereof for use in the manufacture of a medicament for treating a disease or condition selected from the group consisting of asthma, congestion, allergic rhinitis, atopic dermatitis, COPD, dermatitis, inflammatory bowel disease, rheumatoid arthritis, allergic nephritis, conjunctivitis, bronchial asthma, food allergy, systemic mast cell disorder, anaphylactic shock, urticaria, eczema, itching, inflammation, ischemia-reperfusion injury, cerebrovascular disorders, pleuritis, ulcerative colitis, eosinophil-related diseases, such as Churg-Strauss syndrome and sinusitis, and basophile-related diseases, such as basophilic leukemia and basophilic leukocytosis. In certain embodiments of the use, the compound of Formula (I) is selected from one of the representative compounds listed in Table A as set forth in the Examples section.
In another embodiment, the present invention provides a compound of Formula (I) or a pharmaceutically acceptable salt thereof for use in treating a disease or condition from the group consisting of asthma, congestion, allergic rhinitis, atopic dermatitis, COPD, dermatitis, inflammatory bowel disease, rheumatoid arthritis, allergic nephritis, conjunctivitis, bronchial asthma, food allergy, systemic mast cell disorder, anaphylactic shock, urticaria, eczema, itching, inflammation, ischemia-reperfusion injury, cerebrovascular disorders, pleuritis, ulcerative colitis, eosinophil-related diseases, such as Churg-Strauss syndrome and sinusitis, and basophile-related diseases, such as basophilic leukemia and basophilic leukocytosis. In certain embodiments of the use, the compound of Formula (I) is selected from one of the representative compounds listed in Table A as set forth in the Examples section.
In specific embodiments of the methods and uses described above, the compound used in the method or use described above is selected one of the compounds 3T, 3U, 3V, 3W, 3X, 3Y, 3AA, 3AB, 3AC, 3AD, 3AE, 3AF, 4, 4D, 4E, 4F, 5N, 5P, 5R, 5S, 6F, 7C, 8, 8G, 10, 10C, 10D, 10F, 11, 11D, 11E, 11F, 12, 14, 14C, 14D, 14E, 14F, 14G, 14H, 15A, 15B, 15C, 15D, 15E, 15F, 15H, 15i, 15J, 15K, 15L, 15M, 15N, 15o, 15P, 15Q, 15R, 15S, 15T, 15X, 15Z, 15AA, 15AB, 15AC, 15AD, 15AE, 15AF, 15AG, 15AH, 15Ai, 15AJ, 15AK, 15AL, 15AM, 15AN, 15AP, 15AR, 15AU, 15AV, 15AW, 15AX, 15AY, 15AZ, 15BA, 15BB, 15BC, 15BD, 15BE, 15BF, 15BH, 15BJ, 15BK, 15BL, 15BM, 15BN, 15Bo, 15BQ, 15BR, 15BS, 15BT, 15BU, 16, 16F, 16H, 16i, 16J, 16K, 16L, 16N, 16P, 16S, 16T, 16U, 16V, 16W, 16X, 16Y, 16AB, 16AC, 16AD, 16AE, 16AG, 16AH, 16AJ, 16AK, 16AQ, 16AR, 17, 17D, 17E, 17G, 17M, 17S, 17Y, 17Z, 18, 19, 19F, 19G, 19J, 20, 20F, 20G, 20H, 22, 22D, 23, 24H, 24i, 25, 26E, 26F, 26G, 26H, 26i, 26J, 26K, 26L, 26M, 26N, 26o, 26P, 26Q, 26R, 26S, 26T, 26U, 26V, 26W, 26X, 26Y, 26AA, 26AB, 28, 29, 30, 31, 31C, 100, 102, 104-128, 130-136, 143, 145, 148, 155, 156, 160, 162-164, 166, 167, 169, 170, 172, 174-176, 180, 182-191, 198, 199, 204-212, 215, 217-222, 224-229, 231, 232, 234-243, 245-249, and 251-255 or a pharmaceutically acceptable salt thereof.
Combination Therapy
The compounds of Formula (I) or their pharmaceutically acceptable salts may be used in combination, either in a single formulation or co-administered as separate formulations with at least one additional therapeutic agent to treat or prevent the diseases and conditions described herein. These additional therapeutic agents include, but are not limited to: (1) a DP receptor antagonist, such as S-5751 and laropiprant; (2) a corticosteroid, such as triamcinolone acetonide, budesonide, beclomethasone, fluticasone and mometasone; (3) a β2-adrenergic agonist, such as salmeterol, formoterol, arformoterol, terbutaline, metaproterenol, albuterol and the like; (4) a leukotriene modifier, including a leukotriene receptor antagonist, such as montelukast, zafirlukast, pranlukast, or a lipooxygenase inhibitor including 5-lipooxygenase inhibitors and FLAP (5-lipooxygenase activating protein) inhibitors, such as zileuton; (5) an antihistamine such as bromopheniramine, chlorpheniramine, dexchlorpheniramine, triprolidine, clemastine, diphenhydramine, diphenylpyraline, tripelennamine, hydroxyzine, methdilazine, promethazine, trimeprazine, azatadine, cyproheptadine, antazoline, pheniramine pyrilamine, astemizole, terfenadine, loratadine, cetirizine, fexofenadine, descarboethoxyloratadine, and the like; (6) a decongestant, including phenylephrine, phenylpropanolamine, pseudophedrine, oxymetazoline, ephinephrine, naphazoline, xylometazoline, propylhexedrine, or levo-desoxyephedrine; (7) an antiitussive, including codeine, hydrocodone, caramiphen, carbetapentane, or dextramethorphan; (8) another prostaglandin ligand, including prostaglandin F agonist such as latanoprost; misoprostol, enprostil, rioprostil, ornoprostol or rosaprostol; (9) a diuretic; (10) non-steroidal antiinflammatory agents (NSAIDs), such as propionic acid derivatives (alminoprofen, benoxaprofen, bucloxic acid, carprofen, fenbufen, fenoprofen, fluprofen, flurbiprofen, ibuprofen, indoprofen, ketoprofen, miroprofen, naproxen, oxaprozin, pirprofen, pranoprofen, suprofen, tiaprofenic acid, and tioxaprofen), acetic acid derivatives (indomethacin, acemetacin, alclofenac, clidanac, diclofenac, fenclofenac, fenclozic acid, fentiazac, furofenac, ibufenac, isoxepac, oxpinac, sulindac, tiopinac, tolmetin, zidometacin, and zomepirac), fenarnic acid derivatives (flufenamic acid, meclofenamic acid, mefenamic acid, niflumic acid and tolfenamic acid), biphenylcarboxylic acid derivatives (diflunisal and flufenisal), oxicams (isoxicam, piroxicam, sudoxicam and tenoxican), salicylates (acetyl salicylic acid, sulfasalazine) and the pyrazolones (apazone, bezpiperylon, feprazone, mofebutazone, oxyphenbutazone, phenylbutazone); (11) cyclooxygenase-2 (COX-2) inhibitors, such as celecoxib and rofecoxib; (12) inhibitors of phosphodiesterase type IV (PDE-IV) e.g., Ariflo, roflumilast; (13) antagonists of the chemokine receptors, especially CCR-1, CCR-2, and CCR-3; (14) cholesterol lowering agents such as HMG-CoA reductase inhibitors (lovastatin, simvastatin and pravastatin, fluvastatin, atorvastatin, and other statins), sequestrants (cholestyramine and colestipol), nicotinic acid, fenofibric acid derivatives (gemfibrozil, clofibrat, fenofibrate and benzafibrate), and probucol; (15) anti-diabetic agents such as insulin, sulfonylureas, biguanides (metformin), α-glucosidase inhibitors (acarbose) and glitazones (troglitazone, pioglitazone, englitazone, rosiglitazone and the like); (16) preparations of interferon beta (interferon beta-1a, interferon beta-1b); (17) anticholinergic agents, such as muscarinic antagonists (ipratropium bromide and tiotropium bromide), as well as selective muscarinic M3 antagonists; (18) steroids such as beclomethasone, methylprednisolone, betamethasone, prednisone, dexamethasone, and hydrocortisone; (19) triptans commonly used for the treatment of migraine such as sumitriptan and rizatriptan; (20) alendronate and other treatments for osteoporosis; (21) other compounds such as 5-aminosalicylic acid and prodrugs thereof, antimetabolites such as azathioprine and 6-mercaptopurine, cytotoxic cancer chemotherapeutic agents, bradykinin (BK2) antagonists such as FK-3657, TP receptor antagonists such as seratrodast, neurokinin antagonists (NK1/NK2), VLA-4 antagonists, such as those described in U.S. Pat. No. 5,510,332, WO97/03094, WO97/02289, WO96/40781, WO96/22966, WO96/20216, WO96/01644, WO96/06108, WO95/15973 and WO96/31206. In addition, the invention encompasses a method of treating prostaglandin D2 mediated diseases comprising: administration to a patient in need of such treatment a non-toxic therapeutically effective amount of a compound of Formula (I), optionally co-administered with one or more of such ingredients as listed immediately above.
When administering a combination therapy to a patient in need of such administration, the therapeutic agents in the combination, or a pharmaceutical composition or compositions comprising the therapeutic agents, may be administered in any order such as, for example, sequentially, concurrently, together, simultaneously and the like.
In one embodiment, the compound of Formula (I) is administered during a time when the additional therapeutic agent(s) exert their prophylactic or therapeutic effect, or vice versa.
In another embodiment, the compound of Formula (I) and the additional therapeutic agent(s) are administered in doses commonly employed when such agents are used as monotherapy for treating the disorder.
In another embodiment, the compound of Formula (I) and the additional therapeutic agent(s) are administered in doses lower than the doses commonly employed when such agents are used as monotherapy for treating the disorder.
In one embodiment, the compound of Formula (I) and the additional therapeutic agent(s) are present in the same composition, which is suitable for oral administration.
The compound of Formula (I) and the additional therapeutic agent(s) can act additively or synergistically. A synergistic combination may allow the use of lower dosages of one or more agents and/or less frequent administration of one or more agents of a combination therapy. A lower dosage or less frequent administration of one or more agents may lower toxicity of the therapy without reducing the efficacy of the therapy.
The doses and dosage regimen of the additional therapeutic agent(s) used in the combination therapies of the present invention for the treatment or prevention of a disease or disorder can be determined by the attending clinician, taking into consideration the approved doses and dosage regimen in the package insert; the age, sex and general health of the patient; and the type and severity of the viral infection or related disease or disorder.
Another aspect of this invention is a kit comprising a therapeutically effective amount of the compound of Formula (I) or a pharmaceutically acceptable salt of said compound, optionally at least one additional therapeutic agent listed above and a pharmaceutically acceptable carrier, vehicle or diluent.
Methods of Preparing the Compounds of Formula (I)
In general, the compounds in the invention may be produced by a variety of processes known to those skilled in the art and by know, processes analogous thereto. The invention disclosed herein is exemplified by the following preparations and examples which should not be construed to limit the scope of the disclosure. Alternative mechanistic pathways and analogous structures will be apparent to those skilled in the art. The practitioner is not limited to these methods.
One skilled in the art will recognize that one route will be optimized depending on the choice of appendage substituents. Additionally, one skilled in the art will recognize that in some cases the order of steps has to be controlled to avoid functional group incompatability.
The prepared compounds may be analyzed for their composition and purity as well as characterized by standard analytical techniques such as, for example, elemental analysis, NMR, mass spectroscopy and IR spectra.
One skilled in the art will recognize that reagents and solvents actually used may be selected from several reagents and solvents well known in the art to be effective equivalents. Hence, when a specific solvent or reagent is mentioned, it is meant to be an illustrative example of the conditions desirable for that particular reaction scheme or for the preparation described below.
Where NMR data are presented, 1H spectra were obtained on either a Varian VXR-400 (400 MHz, 1H), Varian Gemini-300 (300 MHz), Varian Mercury VX-400 (400 MHz), Bruker-Biospin AV-500 (500 MHz) or Bruker Avarice DRX-500 (500 MHz), and chemical shifts are reported as ppm with number of protons and multiplicities indicated parenthetically. Where LC/MS data are presented, analyses was performed using a 1200 series Agilent 6140 Quadrupole LCMS with a 1.8 μM Zorbax SB-C18 column (10-95% of MeCN—H2O with 0.1% TFA over 2.7 min, 1 mL/min) or with an Applied Biosystems API-150 mass spectrometer and Gemini C18 column (50×4.6 mm, 10-95% CH3CN—H2O with 0.05% TFA over 5 min, 1 mL/min).
The following solvents and reagents may be referred to by their abbreviations in parenthesis:
Me=methyl; Et=ethyl; Pr=propyl; Bu=butyl; t-Bu=tert-butyl; Ph=phenyl, and Ac=acetyl
μl=microliters
Acac=acetylacetone
AcOEt or EtOAc=ethyl acetate
AcOH or HOAc=acetic acid
ACN acetonitrile
aq=aqueous
Ar=aryl
atm=atmosphere
9-BBN=9-borabicyclo[3.3.1]nonane
Bn=benzyl
Boc or BOC=tert-butoxycarbonyl
Bz=benzoyl
Boc=tert-butoxycarbonyl
BINAP=2,2′-bis(diphenylphosphino)-1,1′-bisnaphthyl
cat=catalyst or catalytic
Cbz=benyzloxycarbonyl
DAST=diethylaminosulfur trifluoride
DBU=1,8-Diaza-7-bicyclo[5.4.0]undecene
DCM or CH2Cl2: dichloromethane:
DMAP=4-Dimethylaminopyridine
DIBAL=diisobutylaluminum hydride
DIPEA or Hünig's Base=N,N-diisopropylethylamine
DME=1,2-dimethoxyethane
DMF=dimethylformamide
DMS=dimethylsulfide
DMSO=dimethyl sulfoxide
Dppf=1,1′-bis(diphenylphosphino)ferrocene
EDCI or DEC=1-(3-dimethylaminopropyl)-3-ethylcarbodiimide
g=grams
h=hour
HetAr=heteroaryl
HMDS=1,1,1,3,3,3-hexamethyldisilazane
HATU=N,N,N′,N′-tetramethyl-O-(7-azabenzotriazol-1-yl)uranium
hexafluorophosphate
HOBt=1-hydroxybenzotriazole
Im=imidazole
LAH=lithium aluminum hydride
LDA=lithium diisopropylamide
LCMS=liquid chromatography mass spectrometry
LG=leaving group
min=minute
mg=milligrams
mL=milliliters
mmol=millimoles
MeOH: methanol
MS=mass spectrometry
NBS=N-bromosuccimide
NMR=nuclear magnetic resonance spectroscopy
PG=protecting group
Pyr=pyridine
rac or (±)=racemic mixture or enantiomers
RT or rt=room temperature (ambient, about 25° C.)
sat=saturated
SM=starting material
TBSCl=t-butyldimethylsilyl chloride
TBS=t-butyldimethyl silyl
TEA=triethylamine (Et3N)
TFA=trifluoroacetic acid
TFAA=trifluoroacetic anhydride
THF=tetrahydrofuran
TLC=thin layer chromatography
TMS=trimethylsilyl
Tos or Ts=p-toluenesulfonyl (tosyl)
Tol=toluene
IBMX==3-Isobutyl-1-methylxanthine
HBSS=Hank's balanced salt solution
HEPES=1-[4-(2-Hydroxyethyl)-1-piperazinyl]ethane-2-sulfonic acid
The compounds of this invention can be prepared through the general approach outlined in the following schemes. These schemes are being provided to illustrate the present invention. To assist one in this endeavor, the ordinary practitioner would have full knowledge of literature sources such as Chemical Abstracts; Beilstein, Protective Groups in Organic Synthesis 2nd Edition T. W Greene, P. G. M. Wuts 1991, Wiley and Sons; Comprehensive Organic Transformations, Advanced Organic Chemistry etc.
Scheme 1 shows an approach in which an aminoaniline S1 is treated with a substituted oxoacetic acid (R2=aryl or heteroaryl; R═H; preactivated by a chloroformate, oxalyl chloride or the like) or oxoacetic ester S4 (R2=aryl or heteroaryl; R=alkyl) to provide the quinoxaline S2a (R′═OH). This intermediate is then converted to S2b (wherein R=OTf, Cl, Br or other suitable group by treatment with POCl3, SOCl2, P2O5/Bu4NCl, P2O5/Bu4NBr, Tf2O, PhNTf2 etc.) and coupled with S5 (which has been preactivated via a hydroboration reaction with 9-BBN or similar boron-based reagent; YS=a suitable alkyl, cycloalkyl, aryl or heterocylic linker; R3S=ester or other appropriate group, such a as nitrile or alcohol) to provide S3a. Final conversion to S3b is then achieved by one of many appropriate synthetic methods known to practitioners in the art (such as acid- or base-hydrolysis when R3S=ester, oxidation when R3S=alcohol, hydrolysis when R3S=nitrile etc.). Additionally, when R3S is a nitrile, conversion to a carbon linked tetrazole may be achieved by reaction with an appropriate azide.
Left side transformation, in which A is a functional group such as an ester, nitrile, halogen, optionally functionalized alcohol, sulfonic acid or other group, to A=one of the various definitions of R1, such as amide, ketone, or sulfonamide) occurs by a process known to a practitioner in then art. For example, an activated alcohol or halogen may be carbonylated by a metal catalyzed or metal-facilitated process to provide an ester or acid, which may be further transformed to an amide or ketone. When converting an acid to an amide, an appropriate amine and coupling agent (as EDCI, HOBt, PyBop, HATU etc.) or activation method (oxalyl chloride, thionyl chloride etc.) may be used.




Scheme 2 shows an approach in which S1 is treated with an optionally substituted oxoacetic acid or oxoacetic ester S7 (Y=a suitable alkyl, cycloalkyl, aryl or heterocyclic linker). The resulting product S6a is then converted to S6b (wherein R′=OTf, Cl, Br or other suitable group) and coupled with an appropriately activated, and optionally substituted, partner by a metal catalyzed or metal-facilitated process (such as Stille coupling, Suzuki coupling, Negishi coupling) to provide S3a.




Scheme 3 shows an approach in which S2a is reacted with S9 (in which XS is a leaving group, such as halogen, activated alcohol etc.) and appropriate base (such as LiOtBu, Cs2CO3 DIPEA, LDA, NaH, or other appropriate reagent) to provide S8a.




Scheme 4 shows an alternative sequence in which the quinoxaline synthesis occurs in a stepwise fashion. The nitroaniline S10 is reacted with S4 (R2=aryl or heteroaryl; preactivated by a chloroformate, oxalyl chloride or the like or the like). The resulting ketamide S11 is subjected to nitro reduction (by hydrogenation, or treatment with SnCl2, or other method) and concomitant cyclization to provide S2a.




Scheme 5 shows an approach in which a substituted aminobenzaldehyde S12 is subjected to a Friedlander quinoline synthesis with ketone S14 (in which R2 is aryl or heteroaryl and Y is a linker) and hydroxide base to provide S13.




Scheme 6 shows an approach in which a substituted aminobenzaldehyde S12 is treated with the ketone S16 (in which R2 is aryl or heteroaryl and YS is an alkyl linker) and hydroxide base to provide S15.




Scheme 7 shows an approach in which a substituted aminobenzaldehyde S12 is treated with the substituted acetic acid S19 (in which R2 is aryl or heteroaryl; activated with acetic anhydride or the like) to provide S17a (R′═OH). This intermediate is then converted to S17b (wherein R′=OTf, Cl, Br), coupled with S5 (YS=a suitable linker; R3S=ester) and hydrolyzed to provide the acid S18 as described for Scheme 1.




The starting materials (such as S1, S4, S5, S7, S9, S10, S12, S14, S16, and S19) and reagents used in preparing compounds described are either available from commercial suppliers such as Aldrich Chemical Co. (Wisconsin, USA) and Acros Organics Co. (New Jersey, USA) or were prepared by literature methods known to those skilled in the art.
Compounds, such as those described by formulae S3b, S3c, S8b, S13, S15, and S18b, can be prepared by the general methods outlined above. Exemplary compounds were prepared as described in the examples below or from starting materials known in the art. When unavailable from commercial suppliers, starting materials are synthesized according to methods known in the literature. These examples are being provided to further illustrate the present invention. They are for illustrative purposes only; the scope of the invention is not to be considered limited in any way thereby.
EXAMPLES
Example 1




A solution of pent-4-enoic acid 1A (8.00 g, 80.0 mmol) in DCM (50 mL) was cooled to 0° C. under nitrogen. TFAA (25 mL, 180 mmol) was added and the reaction was stirred for 40 min at 0° C. tert-Butanol (86 mL, 896 mmol) was added and the reaction was warmed to RT and stirred for 16 h. After this time, the reaction was quenched with saturated aqueous NaHCO3 and diluted with Et2O. The aqueous layer was separated and extracted with Et2O. The combined organics were dried (Na2SO4), filtered, and concentrated. The residue was purified by flash chromatography (0% to 20% Et2O/pentane) to yield 1 (4.47 g, yield=18%): 1H NMR (500 MHz, CDCl3) δ 5.92-5.76 (m, 1H), 5.12-4.96 (m, 2H), 2.49-2.43 (m, 1H), 2.42-2.36 (m, 1H), 2.36-2.27 (m, 2H), 1.44 (s, 9H).
Example 2




Step 1
2-(3-Fluorophenyl)-2-oxoacetic Acid




To a stirred solution of ethyl 2-(3-fluorophenyl)-2-oxoacetate (2A) (3.14 g, 16.0 mmol) in THF (120 mL) was added LiOH (1.64 g, 31.9 mmol) in water (40 mL). The reaction was stirred at RT for 1 h. After this time, the reaction was diluted with water and heptane. The aqueous layer was separated, cooled to 0° C., acidified with 1 N HCl to pH 3, and extracted with EtOAc. The combined organics were washed with water, dried (Na2SO4), filtered, and concentrated to yield 2B (2.32 g, yield=86%): 1H NMR (500 MHz, CDCl3) δ 9.30 (br s, 1H), 8.18-8.12 (m, 1H), 8.05-7.96 (m, 1H), 7.60-7.51 (m, 1H), 7.45-7.39 (m, 1H).
Step 2
Methyl 2-(3-Fluorophenyl)-3-oxo-3,4-dihydroquinoxaline-6-carboxylate




To a stirred solution of 2B (1.65 g, 9.81 mmol) and triethylamine (1.37 mL, 9.82 mmol) in THF (3 mL) was added ethyl chloroformate (0.93 mL, 9.76 mmol) at 0° C. under nitrogen. The resulting mixture was stirred at 0° C. for 20 min and a solution of methyl 3,4-diaminobenzoate (1.79 g, 10.8 mmol) in THF (27 mL) was slowly added over 50 min. The reaction was stirred at 0° C. for 1 h, then warmed to RT and stirred for 3 days. After this time, the reaction was concentrated, and the residue was triturated with Et2O and water. The solids were collected by vacuum filtration and the filter cake was washed with Et2O and dried under vacuum to yield 2C (2.56 g, yield 87%): 1H NMR (300 MHz, DMSO-d6) δ 12.82 (s, 1H), 8.26-8.05 (m, 2H), 8.01-7.91 (m, 2H), 7.90-7.80 (m, 1H), 7.64-7.51 (m, 1H), 7.46-7.36 (m, 1H), 3.91 (s, 3H).
Step 3
Methyl 3-Chloro-2-(3-fluorophenyl)quinoxaline-6-carboxylate




A suspension of 2C (2.56 g, 8.58 mmol) and POCl3 (23 mL) was stirred at 110° C. under nitrogen for 24 h. The reaction was cooled to RT and concentrated. The residue was dissolved in DCM and ice was slowly added. The mixture was stirred at 0° C. for 1 h. The aqueous layer was separated and extracted with DCM. The combined organics were dried (Na2SO4), filtered, and concentrated to yield 2D (2.00 g, yield=74%): MS (M+H)=317.
Step 4
Methyl 3-(5-tert-Butoxy-5-oxopentyl)-2-(3-fluorophenyl)quinoxaline-6-carboxylate




To a solution of 1 (1.14 g, 7.30 mmol) in anhydrous THF (10 mL) was added 9-BBN (0.5 M in THF, 14.6 mL, 7.30 mmol) at 0° C. under nitrogen. The reaction was stirred at 0° C. for 30 min and warmed to RT for 3 h. 2D (1.00 g, 3.16 mmol), Pd(dppf)Cl2.CH2Cl2 (310 mg, 0.380 mmol), and K3PO4 (1.88 g, 8.86 mmol) were added. The suspension was degassed (3× vacuum/nitrogen) and heated at 60° C. for 18 h. After this time, the reaction was cooled to RT and filtered. The filtrate was diluted with DCM and water. The aqueous layer was separated and extracted with DCM. The combined organics were dried (Na2SO4), filtered, and concentrated. The residue was purified by column chromatography (10% EtOAc/heptane) to yield 2E (1.43 g, yield=>99%): MS (M+H)=439.
Step 5
3-(5-tert-Butoxy-5-oxopentyl)-2-(3-fluorophenyl)quinoxaline-6-carboxylic Acid




A solution of 2E (1.43 g, 3.16 mmol) and LiOH (265 mg, 6.32 mmol) in THF (20 mL) and water (10 mL) was stirred at RT for 1 h. After this time, the reaction was diluted with water and extracted with heptane. The aqueous layer was acidified with 1 N HCl, then extracted with DCM. The combined organics were washed with water, dried (Na2SO4), filtered, and concentrated to yield 2 (1.13 g, yield=84%): MS (M+H)=425.
In a manner similar to that described above, compound 2F was treated with LiOH to provide 2G (1H NMR, 300 MHz, CDCl3 δ 8.48 (d, J=8.1 Hz, 2H), 7.81 (d, J=8.3 Hz, 2H)) and then reacted with methyl 3,4-diaminobenzoate. The resulting 3-oxo-quinoxaline 211 (MS: M+H=349) was sequentially reacted with POCl3 (to provide 2i, MS: M+H 367), coupled with compound 1 (to provide 2J, MS: M+H 489), and hydrolyzed with LiOH to provide 2K (MS: M+H=475)




Example 3




Step 1
tert-Butyl 5-{3-(3-Fluorophenyl)-7-[methoxy(methyl)carbamoyl]quinoxalin-2-yl}pentanoate




A solution of 2 (304 mg, 0.716 mmol), O,N-dimethylhydroxylamine hydrochloride (175 mg, 1.79 mmol), diisopropylethylamine (0.62 mL, 3.56 mmol), and BOP—Cl (547 mg, 2.15 mmol) in anhydrous THF (10 mL) was stirred at RT for 18 h under nitrogen. After this time, the reaction was diluted with EtOAc and washed with water. The organic layer was separated, dried (Na2SO4), filtered, and concentrated. The residue was purified by column chromatography (30% to 50% EtOAc/heptane) to yield 3A (308 mg, yield=92%): MS (M+H)=468.
Step 2
tert-Butyl 5-[7-(Cyclopropanecarboyl)-3-(3-fluorophenyl)quinoxalin-2-yl]pentanoate




To a stirred solution of 3A (150 mg, 0.321 mmol) in anhydrous THF (1 mL) was added cyclopropylmagnesium bromide (0.5 M in THF, 1.93 mL, 0.965 mmol) at −40° C. under nitrogen. After the addition, the reaction was warmed to 10° C. over 1 h. After this time, the reaction was quenched with saturated aqueous NH4Cl and extracted with EtOAc. The combined organics were dried (Na2SO4), filtered, and concentrated. The residue was purified by column chromatography (30% EtOAc/heptane) to yield 3B (102 mg, yield=71%): MS (M+H)=449.
Step 3
5-[7-(Cyclopropanecarbonyl)-3-(3-fluorophenyl)quinoxalin-2-yl]pentanoic Acid




To a solution of 3B (102 mg, 0.227 mmol) in DCM (2 mL) was added TFA (1 mL) and the solution stirred for 1.5 h at RT. After this time, the reaction was concentrated and the residue was triturated with a mixture of water (0.1 mL), DCM (3 mL), and heptane (20 mL). The solids were collected by vacuum filtration and the filter cake was dissolved in 2:2:0.2 CH3CN/H2O/1 M NH4OH (2.2 mL), and lyophilized to yield 3 (70 mg, yield=75%): MS (M+H)=393.
In a manner similar to that described for 3A above, compound 2 was coupled with the appropriate amine to provide the following compounds:













Compound





Number
Coupling Partner
Compound
M + H







3C








518


 


3D








518


 


3E








518









In a manner similar to that described for 3A above, compound 2K was coupled with the appropriate amine to provide the following compounds:













Compound





Number
Coupling Partner
Compound
M + H







3F








578


 


3G








578


 


3H








619


 


3i








518


 


3J








579


 


3K








579


 


3L








656


 


3M








656


 


3N








656


 


3o








565


 


3P








565


 


3Q








565


 


3R








633









In a manner similar to that described for 3B above, compound 3i was reacted with cyclopropylmagnesium bromide to provide the following compound:













Compound
Starting




Number
Material
Compound
M + H







3S
3i




499









In a manner similar to that described above for compound 3, the following compounds were prepared from the TFA deprotection of the indicated starting materials (SM):














No.
SM
Compound

M + H







3T
3C




3-(3-fluorophenyl)-7-[[(4- fluorophenyl)amino]carbonyl]- 2-quinoxalinepentanoic acid
462


 


3U
3D




3-(3-fluorophenyl)-7-[[(3- fluorophenyl)amino]carbonyl]- 2-quinoxalinepentanoic acid
462


 


3V
3E




3-(3-fluoropheny)-7-[[(2- fluorophenyl)amino]carbonyl]- 2-quinoxalinepentanoic acid
462


 


3W
3F




7-[[(1-(S)- phenylethyl)amino]carbonyl]- 3-[4-(trifluoromethyl)phenyl]- 2-quinoxalinepentanoic acid
522


 


3X
3G




7-[[(1-(R)- phenylethyl)amino]carbonyl]- 3-[4-(trifluoromethyl)phenyl]- 2-quinoxalinepentanoic acid
522


 


3Y
3H




7-[(4-phenyl-1- piperazinyl)carbonyl]-3-[4- (trifluoromethyl)phenyl]-2- quinoxalinepentanoic acid
563


 


3Z
3S




7-(cyclopropylcarbonyl)-3-[4- (trifluoromethyl)phenyl]-2- quinoxalinepentanoic acid
443


 


3AA
3J




7-[[[1-(4-pyridinyl)ethyl]- amino]carbonyl]-3-[4- (trifluoromethyl)-phenyl]-2- quinoxaline-pentanoic acid
523


 


3AB
3K




7-[[[1-(2-pyridinyl)ethyl]- amino]carbonyl]-3-[4- (trifluoromethyl)phenyl]-2- quinoxalinepentanoic acid
523


 


3AC
3o




7-[[(4-pyridinylmethyl)- amino]carbonyl]-3-[4- (trifluoromethyl)phenyl]-2- quinoxalinepentanoic acid
509


 


3AD
3P




5-(7-pyridin-3- ylmethylcarbamoyl)-3-(4- (trifluoromethyl)phenyl) quinoxalin-2-yl)pentanoic acid
509


 


3AE
3Q




7-[[(2-pyridinylmethyl)- amino]carbonyl]-3-[4- (trifluoromethyl)phenyl]-2- quinoxalinepentanoic acid
509


 


3AF
3R




3-[4-(trifluoromethyl)phenyl]- 7-[[[[6-(trifluoromethyl)-3- pyridinyl]methyl]amino]- carbonyl]-2-quinoxaline- pentanoic acid
577


 


3AG
2J




7-(methoxycarbonyl)-3-[4- (trifluoromethyl)phenyl]-2- quinoxalinepentanoic acid
433









Example 4




Step 1
(S)-tert-Butyl 5-{7-[1-(biphenyl-3-yl)ethylcarbamoyl]-3-[4-(trifluoromethyl)-phenyl]quinoxalin-2-yl}pentanoate




A stirred solution of 3L (59 mg, 0.090 mmol), phenylboronic acid (11 mg, 0.090 mmol), Pd(PPh3)4 (0.46 mg, 0.005 mmol), and sodium carbonate (25 mg, 0.225 mmol) in toluene (2 mL) and water (0.1 mL) was heated at reflux for 16 h. After this time, the reaction was cooled to RT, filtered through a short pad of CELITE, and the filter cake was washed with EtOAc. The filtrate was diluted with EtOAc, washed with water, dried (Na2SO4), filtered, and concentrated to yield 4A (47 mg, yield=79%): MS (M+H)=654.
Step 2
(S)-5-{7-[1-(biphenyl-3-yl)ethylcarbamoyl]-3-[4-(trifluoromethyl)phenyl]quinoxalin-2-yl}pentanoic Acid




To a stirred solution of 4A (47 mg, 0.071 mmol) in DCM (2 mL) was added TFA (1 mL) and the solution stirred for 1.5 h at RT. After this time, the reaction was concentrated and the residue was triturated with a mixture of water (0.1 mL), DCM (3 mL) and heptane (20 mL). The solids collected by vacuum filtration and the filter cake was dissolved in 2:2:0.2 CH3CN/H2O/1 M NH4OH (2.2 mL) and lyophilized to yield 4 (21 mg, yield=47%): MS (M+H)=598.
In a manner similar to that described above for 4A, the following compounds were prepared:













Compound
Starting




Number
Material
Compound 
M + H







4B
3M




654


 


4C
3N




654









In a manner similar to that described above for compound 4, the following compounds were prepared via a TFA deprotection of the indicated starting materials (SM):














No.
SM
Compound
Name
M + H







4D
4B




7-[[[1-(R)-[1,1′- biphenyl]-3-ylethyl] amino]carbonyl]-3- [4-(trifluoromethyl)- phenyl]-2- quinoxaline- pentanoic acid
598


 


4E
4C




7-[[(1-[1,1′-biphenyl]- 4-ylethyl)amino] carbonyl]-3-[4- (trifluoromethyl)- phenyl]-2- quinoxaline- pentanoic acid
598









The racemic mixture 4E was separated on a semi-preparative Chiralpak IC column (15% ethanol-hexanes-0.1% diethylamine) to provide the pure enantiomers 4F (Enantiomer 1, >99% ee, LCMS: M+H=598) and 4G (Enantiomer 2, >99% ee, LCMS: M+H=598).
Example 5




Step 1
Methyl 2-(4-fluorophenyl)-3-oxo-3,4-dihydroquinoxaline-6-carboxylate




To a solution of 2-(4-fluorophenyl)-2-oxoacetic acid (507 mg, 3.02 mmol, prepared from ethyl 2-(4-fluorophenyl)-2-oxoacetate as described in Example 2) and triethylamine (0.42 mL, 3.0 mmol) cooled to 0° C. was added ethyl chloroformate (0.29 mL, 3.0 mmol). The resulting mixture was stirred at 0° C. for 20 minutes and methyl 3,4-diaminobenzoate (551 mg, 3.32 mmol) was added slowly over 50 minutes as a solution in THF (10 mL). The reaction was stirred at 0° C. for 1 h and at RT for 72 h. The reaction was concentrated to remove most of the THF. The solid was diluted with Et2O and water and cooled to 0° C. The solid was filtered and washed with Et2O (1×20 mL) and DCM (1×20 mL) to yield methyl 2-(4-fluorophenyl)-3-oxo-3,4-dihydroquinoxaline-6-carboxylate (5A, 665 mg; Yield=73.9%). 1H NMR (300 MHz, DMSO-d6) δ 12.77 (s, 1H), 8.50-8.38 (m, 2H), 7.98-7.89 (m, 2H), 7.84 (dd, J=8.5, 1.8 Hz, 1H), 7.41-7.29 (m, 2H), 3.91 (s, 3H).
Step 2
Methyl 3-chloro-2-(4-fluorophenyl)quinoxaline-6-carboxylate




A suspension of methyl 2-(4-fluorophenyl)-3-oxo-3,4-dihydroquinoxaline-6-carboxylate (5A, 2.20 g, 7.38 mmol), phosphoryl chloride (20 mL, 200 mmol), and was degassed and stirred at 110° C. for 40 h. The reaction was cooled to RT, some of the excess POCl3 was concentrated, and then cooled to at 0° C. The mixture was diluted with DCM and ice was added slowly. This mixture was stirred at 0° C. for 1 h. The organic layer was removed and the aqueous phase was extracted with DCM (3×). The combined organics were dried (Na2SO4), filtered, and concentrated. The residue was purified by flash chromatography (0% to 50% EtOAc/hexanes) to yield methyl 3-chloro-2-(4-fluorophenyl)quinoxaline-6-carboxylate (5B, 2.1 g; Yield=90%), MS (M+H)=317.
Step 3
Methyl 3-(5-tert-butoxy-5-oxopentyl)-2-(4-fluorophenyl)quinoxaline-6-carboxylate




To a solution of tert-butyl pent-4-enoate (1, 1.5 g, 9.8 mmol) in THF (10 mL, 100 mmol) cooled to 0° C. was added 0.5 M of 9-Borabicyclo[3.3.1]nonane in THF (19.6 mL). The reaction was stirred at 0° C. for 30 minutes and at RT for 3 h. To this solution was added methyl 3-chloro-2-(4-fluorophenyl)quinoxaline-6-carboxylate (5B, 1.33 g, 4.20 mmol), [1,1′-Bis(diphenylphosphino)ferrocene]dichloropalladium(II)-complex with dichloromethane (1:1) (0.423 g, 0.518 mmol), and potassium phosphate (2.5 g, 12 mmol). The resulting solution was degassed and stirred at 60° C. for 16 h. The filtrate was diluted with DCM and water. The organic layer was removed and the aqueous phase was extracted with DCM (2×). The combined organics were dried (Na2SO4), filtered, and concentrated. The residue was purified by flash chromatography (0% to 60% EtOAc/hexanes) to yield methyl 3-(5-tert-butoxy-5-oxopentyl)-2-(4-fluorophenyl)quinoxaline-6-carboxylate (5C, 1.82 g; Yield=98%), MS (M+H)=439.
Step 4
3-(5-tert-butoxy-5-oxopentyl)-2-(4-fluorophenyl)quinoxaline-6-carboxylic acid




To a solution of methyl 3-(5-tert-butoxy-5-oxopentyl)-2-(4-fluorophenyl)quinoxaline-6-carboxylate (5C, 1.82 g, 4.15 mmol) in THF (30 mL, 400 mmol) and water (15 mL, 830 mmol) was added lithium hydroxide monohydrate (352 mg, 8.39 mmol). The reaction was stirred at RT for 1 h and quenched with 0.1N HCl. The reaction was diluted with EtOAc and the organic layer was removed. The aqueous phase was extracted with EtOAc (3×) and the combined organics were dried (Na2SO4), filtered, and concentrated to yield 5D (1.67 g; Yield=94.8%, MS: M+H=425). The material was used without purification.
Step 5
tert-butyl 5-(3-(4-fluorophenyl)-7-(methoxy(methyl)carbamoyl)quinoxalin-2-yl)pentanoate




To a solution of crude 3-(5-tert-butoxy-5-oxopentyl)-2-(4-fluorophenyl)quinoxaline-6-carboxylic acid (5D, 1.67 g, 3.93 mmol), N,O-dimethylhydroxylamine hydrochloride (534 mg, 5.47 mmol), and N,N-diisopropylethylamine (2.40 mL, 13.8 mmol) in DCM (40 mL, 600 mmol) was added 1-hydroxybenzotriazole (0.802 g, 5.94 mmol) and N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (1.53 g, 7.98 mmol). The reaction was stirred at RT for 18 h. The reaction was diluted with 0.3N HCl. The organic layer was removed and the aqueous phase was extracted with DCM (3×). The combined organics were dried (Na2SO4), filtered, and concentrated. The residue was purified by flash chromatography (0% to 100% EtOAc/hexanes) to yield tert-butyl 5-(3-(4-fluorophenyl)-7-(methoxy(methyl)carbamoyl)quinoxalin-2-yl)pentanoate (5E, 1.50 g; Yield=81.5%), MS (M+H)=468.
Step 6
tert-butyl 5-(7-(cyclopropanecarbonyl)-3-(4-fluorophenyl)quinoxalin-2-yl)pentanoate




To a solution of tert-butyl 5-(3-(4-fluorophenyl)-7-(methoxy(methyl)carbamoyl)quinoxalin-2-yl)pentanoate (5E, 80 mg, 0.2 mmol) in THF (2 mL, 20 mmol) cooled to −40° C. was added 0.5 M of cyclopropylmagnesium bromide in THF (0.530 mL). The reaction was stirred for 45 minutes gradually warming to 10° C. The reaction was quenched with dilute HCl and diluted with DCM. The organic layer was removed and the aqueous phase was extracted with DCM (3×). The combined organics were concentrated. The residue was purified by flash chromatography (0% to 70% EtOAc/hexanes) to yield tert-butyl 5-(7-(cyclopropanecarbonyl)-3-(4-fluorophenyl)quinoxalin-2-yl)pentanoate (5F, 58 mg; Yield=80%), MS (M+H)=449.
Step 7
5-(7-(cyclopropanecarbonyl)-3-(4-fluorophenyl)quinoxalin-2-yl)pentanoic acid




A solution of tert-butyl 5-(7-(cyclopropanecarbonyl)-3-(4-fluorophenyl)quinoxalin-2-yl)pentanoate (5F, 57 mg, 0.13 mmol) and TFA (0.4 mL, 5 mmol) in DCM (1.6 mL, 25 mmol) was stirred for 2 h. The residue was purified by reverse phase chromatography (10:90 to 100:00 CH3CN/H2O (0.1% TFA)) to yield 5-(7-(cyclopropanecarbonyl)-3-(4-fluorophenyl)quinoxalin-2-yl)pentanoic acid (5). MS (M+H)=393.
In a manner similar to that described above, the compound 5G (MS, M+H=429) was prepared from 5E:




In a manner similar to that described for 3A above, compound 5D was coupled with the appropriate amine to provide the following compounds:













Compound





Number
Coupling Partner
Compound
M + H







5H








518


 


5i








518


 


5J








518


 


5K








452


 


5L








466


 


5M








464









In a manner similar to that described above, the following compounds were prepared from the TFA deprotection of the indicated starting materials (SM):














No.
SM
Compound
Name
M + H







5N
5H




3-(4-fluorophenyl)-7-[[(3- fluorophenyl)amino]carbonyl]-2- quinoxalinepentanoic acid
462


 


5o
5i




3-(4-fluorophenyl)-7-[[(4- fluorophenyl)amino]carbonyl]-2- quinoxalinepentanoic acid
462


 


5P
5J




3-(4-fluorophenyl)-7-[[(2- fluorophenyl)amino]carbonyl]-2- quinoxalinepentanoic acid
462


 


5Q
5K




7-[(dimethylamino)carbonyl]-3- (4-fluorophenyl)-2- quinoxalinepentanoic acid
396


 


5R
5L




3-(4-fluorophenyl)-7-[[(1- methylethyl)amino]carbonyl]-2- quinoxalinepentanoic acid
410


 


5S
5M




7-(1-azetidinylcarbonyl)-3-(4- fluorophenyl)-2- quinoxalinepentanoic acid
408









Example 6




In a manner similar to that described in Example 5, methyl 3,4-diaminobenzoate was reacted with 2-oxo-2-phenylacetic acid. The resulting product 6A was then advanced to compound to 6E, which was used to prepare the following compounds:













No.
Compound
Name
M + H







6




7-(1-oxopropyl)-3-phenyl-2- quinoxalinepentanoic acid
363


 


6F




7-benzoyl-3-phenyl-2- quinoxalinepentanoic acid
411


 


6G




7-(cyclopropylcarbonyl)- 3-phenyl-2- quinoxalinepentanoic acid
375









In a manner similar to that previously described, 6C was deprotected with TFA to provide 7-(methoxycarbonyl)-3-phenyl-2-quinoxalinepentanoic acid 6H (MS, M+H=365).




Example 7




Step 1
tert-Butyl 5-[3-(4-Fluorophenyl)-7-(methylcarbamoyl)quinoxalin-2-yl]pentanoate




To a stirred solution of 5E (154 mg, 0.329 mmol) in anhydrous THF (5 mL) was added tert-butylmagnesium chloride (2 M in THF, 0.330 mL, 0.660 mmol) at −20° C. under nitrogen. The reaction mixture was slowly warmed to 10° C. over 40 min and then quenched with saturated aqueous NH4Cl. The resulting mixture was extracted with DCM. The combined extracts were dried (Na2SO4), filtered, and concentrated. The residue was purified by column chromatography (50% to 70% EtOAc/heptane) to yield 7A (58 mg, yield=40%): MS (M+H)=438.
Step 2
5-[3-(4-Fluorophenyl)-7-(methylcarbamoyl)quinoxalin-2-yl]pentanoic Acid




A solution of 7A (58 mg, 0.133 mmol) and TFA (1 mL) in ACM (2 mL) was stirred at RT for 2 h. After this time, the reaction mixture was concentrated and the resulting residue was purified by column chromatography (2% to 10% MeOH/DCM) and lyophilized from CH3CN (2 mL), H2O (1 mL), and 1 M NH4OH (0.2 mL) to yield 3-(4-fluorophenyl)-7-[(methylamino)carbonyl]-2-quinoxalinepentanoic acid 7 (43 mg, yield=81%): MS (M+H)=382.
In a manner similar to that described above, compound 5E was reacted with isopropylmagnesium bromide to provide 7B (MS, M+H=451) and then deprotected with TFA to provide 3-(4-fluorophenyl)-7-(2-methyl-1-oxopropyl)-2-quinoxalinepentanoic acid 7C (MS, M+H=395):




Example 8




Step 1
Methyl 3-Nitro-4-{[oxo(phenyl)acetyl]amino}benzoate




Following the addition of DMF (0.030 mL, 0.382 mmol) to a chilled solution of benzoylformic acid (1.26 g, 8.41 mmol) in THF (19 mL) at 0° C., oxalyl chloride (0.74 mL, 8.4 mmol) was added dropwise (to give vigorous bubbling) and the reaction mixture was stirred at 0° C. for 30 min, then allowed to warm to RT over 2 h. The reaction solution was then added dropwise to a chilled solution of methyl 4-amino-3-nitrobenzoate (8A, 1.5 g, 7.7 mmol) and triethylamine (2.1 mL, 15 mmol) in THF (57 mL) at 0° C. and allowed to warm to RT overnight. The reaction was partitioned between EtOAc and aqueous NaHCO3 (sat'd). The organics were then washed with brine, dried over Na2SO4, and concentrated to give a yellow solid 8B.
Step 2
Methyl 2-Oxo-3-phenyl-1,2-dihydroquinoxaline-6-carboxylate




A solution of the yellow solid 8B (2.45 g, 7.46 mmol) and Pt/V (0.73 g, 0.037 mmol) in THF (50 mL) and MeOH (50 mL) was stirred under an atmosphere of hydrogen (balloon) at RT for 30 min. The reaction was filtered through CELITE and concentrated to a yellow solid. Purification by chromatography on SiO2 (0-20% EtOAc/DCM) gave 8C. 1H NMR (500 MHz, DMSO-d6) δ 12.85 (s, 1H), 8.33 (s, 1H), 8.29 (d, J=8.1 Hz, 2H), 8.06 (d, J=8.6 Hz, 1H), 7.48-7.52 (m, 3H), 7.39 (d, J=8.6 Hz, 1H), 3.88 (s, 3H); MS (M+H)=281.
Step 3
Methyl 2-Chloro-3-phenylquinoxaline-6-carboxylate




A suspension of 8C (300 mg, 1.1 mmol) in POCl3 (3 mL) was stirred overnight at 110° C. and concentrated to dryness. The residue was dissolved in DCM and extracted with 1 N NaOH, water, dried (Na2SO4), and concentrated. Purification by chromatography on SiO2 (0-50% EtOAc/DCM) gave 8D. 1H NMR (500 MHz, CDCl3) δ 8.87 (s, 1H), 8.39 (d, J=8.8 Hz, 1H), 8.11 (d, J=8.6 Hz, 1H), 7.87-7.89 (m, 2H), 7.55-7.56 (m, 3H), 4.02 (s, 3H); MS (M+H)=299.
Step 4
Methyl 2-(5-tert-Butoxy-5-oxopentyl)-3-phenylquinoxaline-6-carboxylate




A chilled solution of tert-butyl pent-4-enoate (1, 230 mg, 1.47 mmol) in THF (2 mL) at 0° C. was charged with a 0.5 M solution of 9-BBN in THF (3.0 mL, 1.47 mmol) and stirred at 0° C. for 30 min. The reaction was allowed to warm to RT over 2.5 h, then added to a flask containing 8D (110 mg, 0.368 mmol), PdCl2(dppf)-CH2Cl2 (39 mg, 0.048 mmol), and K3PO4 (190 mg, 0.88 mmol). The new solution was degassed for 15 min by bubbling nitrogen gas, heated to 60° C., and stirred overnight. The reaction was diluted with DCM and water and the layers were then separated. The aqueous layer was then extracted with DCM. Organics were combined, dried (Na2SO4), and concentrated. Chromatography on SiO2 (0-50% EtOAc/hexanes) provided 8E. MS (M+H)=421.
Step 5
2-(5-tert-Butoxy-5-oxopentyl)-3-phenylquinoxaline-6-carboxylic Acid




A solution of the methyl ester 5E (85 mg, 0.20 mmol) in 1 mL of THF was charged with a 1 M solution of LiOH in water (1 mL) After stirring for 2 hours, the reaction mixture was diluted with EtOAc, extracted with 1 N HCl, water, dried (Na2SO4) and concentrated to give 8F. MS (M+H)=407.
Step 6
5-(6-{[(3-Chlorobenzyl)oxy]carbonyl}-3-phenylquinoxalin-2-yl)pentanoic Acid




A solution of the acid 8F (20 mg, 0.049 mmol), 3-chlorobenzylamine (9 μL, 0.07 mmol), HOBT (11 mg, 0.074 mmol), and EDC (19 mg, 0.098 mmol) in DCM (1 mL) was stirred at RT overnight. The reaction was charged with TFA (160 μL) and stirred for 2 h and concentrated. Reverse phase chromatography (MeCN/water) provided the desired final product 8. 1H NMR (500 MHz, DMSO-d6) δ 9.44 (t, J=5.9 Hz, 1H), 8.62 (s, 1H), 8.25 (d, J=8.8 Hz, 1H), 8.14 (d, J=8.8 Hz, 1H), 7.69 (m, 2H), 7.55 (m, 3H), 7.30-7.40 (m, 3H), 4.53 (d, J=6.1 Hz, 2H), 3.02 (t, J=7.4 Hz, 2H), 2.14 (t, J=7.5 Hz, 2H), 1.71 (t, J=7.5 Hz, 2H), 1.46 (m, 2H); MS (M+H)=474.
In a manner similar to that described for the synthesis of 8, compound 8G (MS, M+H=472) was prepared by coupling acid 8F to (R)-1-(4-fluorophenyl)-ethanamine followed by a TFA deprotection




Example 9




Step 1
Methyl 3-(4-tert-Butoxy-4-oxobutoxy)-2-phenylquinoxaline-6-carboxylate




A suspension of 6A (300 mg, 1.1 mmol) and Cs2CO3 (500 mg, 1.5 mmol) in 5 mL of DMF was treated with tert-butyl 4-bromobutanoate (300 mg, 1.3 mmol) and stirred for 3 hours at 100° C. Diluted with DCM, extracted with 2 N HCl, water, dried, conc. Chromatography on SiO2 (0-100% EtOAc/hexanes) gave the desired O-alkylation product 9A along with a small amount of undesired N-alkylated product. MS (M+H)=423.
Step 2
4-{[7-(Methoxycarbonyl)-3-phenylquinoxalin-2-yl]oxy}butanoic Acid




The tert-butyl ester 9A (100 mg) was dissolved in 1 mL of DCM and 1 mL of TFA, stirred 1 hour and concentrated. Reverse-phase chromatography (MeCN/water) provided the desired title product 9. 1H-NMR (500 MHz, DMSO-d6) δ 12.2 (s, 1H), 8.36 (s, 1H), 8.06-8.14 (m, 4H), 7.54 (m, 3H), 4.53 (t, J=6.3 Hz Hz, 2H), 3.92 (s, 3H), 2.41 (t, J=7.3 Hz, 2H); 2.04 (m, 2H); MS (M+H)=367.
Example 10




Step 1
3-(4-tert-Butoxy-4-oxobutoxy)-2-phenylquinoxaline-6-carboxylic Acid




A solution of the methyl ester 9A (200 mg, 0.47 mmol) in 3 mL of THF and 1 mL of water was treated with LiOH (20 mg, 0.84 mmol) and stirred overnight. The reaction mixture was diluted with DCM and extracted with 1 N HCl, water, dried (Na2SO4), and concentrated to provide the intermediate acid 10A.
Step 2
tert-Butyl 4-({3-Phenyl-7-[(pyridin-3-ylmethyl)carbamoyl]quinoxalin-2-yl}oxy)butanoate




A solution of the acid 10A (20 mg, 0.05 mmol), 1-(pyridin-3-yl)methanamine (15 mg, 0.14 mmol), EDC (20 mg, 0.10 mmol) and HOBt (10 mg, 0.065 mmol) in 1 mL of DCM was stirred overnight. The reaction mixture was diluted with EtOAc, washed with 2 N HCl, 2 N NaOH, and water, dried (Na2SO4), and concentrated to provide 10B.
Step 3
4-({3-Phenyl-7-[(pyridin-3-ylmethyl)carbamoyl]quinoxalin-2-yl}oxy)butanoic Acid




A solution of the intermediate tert-butyl ester 10B (24 mg) in 1 mL of DCM and 1 mL of TFA was stirred for 1 hour and concentrated. The residue was purified by reverse phase chromatography (MeCN/water) to provide the final acid 10. MS (M+H)=443.
In a manner similar to that described for the synthesis of 10, the following compounds were prepared by coupling acid 10A to the appropriate amine reagent followed by a TFA deprotection:














No.
Reagent
Compound Structure
Name
M + H







10C








4-({7-[(4-chlorobenzyl) carbamoyl]-3- phenylquinoxalin- 2-yl}oxy)butanoic acid
476


 


10D








4-[(3-phenyl-7-{[(1S)-1- phenylethyl]carbamoyl} quinoxalin-2-yl)oxy] butanoic acid
456


 


10E








4-({7-[benzyl(methyl) carbamoyl]-3- phenylquinoxalin-2-yl} oxy)butanoic acid
456


 


10F








4-{[7-(3,4-dihydroiso- quinolin-2(1H)- ylcarbonyl)-3- phenylquinoxalin- 2-yl]oxy} butanoic acid
468









Example 11




Step 1
tert-Butyl 5-{7-[(tert-Butoxycarbonyl)amino]-3-phenylquinoxalin-2-yl}pentanoate




To a flask containing 3-(5-tert-butoxy-5-oxopentyl)-2-phenylquinoxaline-6-carboxylic acid 6D (0.8 g, 1.97 mmol) was added tBuOH (13 mL), Et3N (6.0 eq), and diphenylphosphoryl azide (2.0 eq). The mixture was heated at reflux for 20 h. The mixture was cooled to RT, and concentrated to remove most of tBuOH, then diluted with EtOAc and sat. NaHCO3. The organic layer was separated and the aqueous layer was extracted with EtOAc. The combined organic layers were washed with brine, dried over MgSO4. Concentration and flash chromatography (0-15% EtOAc/CH2Cl2) gave 11A. MS (M+H)=478.
Step 2
Methyl 5-(7-amino-3-phenylquinoxalin-2-yl)pentanoate




To a solution tert-butyl 5-{7-[(tert-butoxycarbonyl)amino]-3-phenylquinoxalin-2-yl}pentanoate 11A (0.5 g, 1.1 mmol) in DCM (6 mL) at RT was added. TFA (2 mL), and the mixture was stirred at RT for 4 h. The mixture was concentrated under reduced pressure and the crude residue was taken up in MeOH (0.15M). Then SOCl2 (3 eq) was added at 0° C. and the reaction mixture was stirred at the same temperature for 1.5 h. The mixture was then diluted with EtOAc, and quenched slowly with sat. NaHCO3. The organic layer was separated, and the aqueous layer was extracted with EtOAc. The combined organics were washed with H2O, brine, and dried over MgSO4. Concentration afforded the title compound, which was contaminated by some minor impurities. This material 11B was used for next step without purification. MS (M+H)=336.
Step 3
Methyl 5-(7-{[(3,4-Dichlorophenyl)carbonyl]amino}-3-phenylquinoxalin-2-yl)pentanoate




To a solution of methyl 5-(7-amino-3-phenylquinoxalin-2-yl)pentanoate 11B (50 mg, 0.15 mmol) in CH2Cl2 (2 mL) was added 3,4-dichlorophenylacetic acid (1.0 eq), Hunig's Base (3.0 eq), HOBt (1.2 eq), and EDC (1.4 eq). The mixture was stirred at ambient temperature overnight, and purified without workup by flash chromatography to afford 11C. MS (M+H)=522.
Step 4
5-(7-{[(3,4-Dichlorophenyl)carbonyl]amino}-3-phenylquinoxalin-2-yl)pentanoic Acid




To a solution of methyl 5-(7-{[(3,4-dichlorophenyl)carbonyl]amino}-3-phenylquinoxalin-2-yl)pentanoate 11C (68 mg, 0.13 mmol) in a mixture of THF (1.5 mL) and MeOH (0.7 mL) was added 1N NaOH (3 eq). The mixture was stirred at RT for 4 h, and then acidified with 1N HCl. The mixture was purified by reverse chromatography (MeCN/water) to provide the desired acid 11. 1H-NMR (600 MHz, DMSO-d6) δ 12.0 (brs, 1H), 10.72 (s, 1H), 8.52 (s, 1H), 8.03 (d, J=9.0 Hz, 1H), 7.86 (d, J=7.8 Hz, 1H), 7.62-7.70 (m, 4H), 7.52-7.60 (m, 3H), 7.40 (d, J=8.4 Hz, 1H), 3.85 (s, 2H), 2.99 (t, J=7.2 Hz, 2H), 2.17 (t, J=7.2 Hz, 2H), 1.69-1.74 (m, 2H), 1.45-1.51 (m, 2H). MS (M+H)=508.
In a manner similar to that described for the synthesis of 11, the following compounds were prepared by coupling aniline 11B to the appropriate acid reagent followed by ester hydrolysis:














No.
Reagent
Compound Structure
Name
M + H







11D








5-[3-phenyl-7- ({[4-(trifluoro- methoxy)phenyl] carbonyl- amino)quinoxalin- 2-yl]- pentanoic acid
510


 


11E








5-(7-{[(4- chlorophenyl)- carbonyl]amino}- 3-phenyl- quinoxalin- 2-yl)pentanoic acid
460


 


11F








5-[3-phenyl-7-({[4- (trifluoromethyl) phenyl]acetyl} amino)quinoxalin- 2-yl]pentanoic acid
508









Example 12




Triethylamine (0.083 mL, 0.596 mmol) and alpha-toluenesulfonyl chloride (56.8 mg, 0.298 mmol) were added to methyl 5-(7-amino-3-phenylquinoxalin-2-yl)pentanoate (11B, 50 mg, 0.149 mmol) stirred in CH2Cl2 (1 mL) and the mixture was stirred at RT for 1 h. The reaction was then concentrated. The residue was then diluted with THF and NaOH (0.5 mL, 0.500 mmol) along with MeOH (0.05) were then added. The mixture was stirred at RT for overnight. The next day the reaction was concentrated in vacuo and then 1N HCl (0.5 mL) was added before the reaction was concentrated again. The residue was then diluted with MeOH and purified (reversed phase C18 HPLC eluting with 1% TFA acetonitrile/water gradient and then column chromatography on silica gel Biotage SNAP KP-Sil 10 g, eluting with EtOAc/hexanes and flushed with 5% MeOH/ethyl acetate) to provide 5-{7-[(benzylsulfonyl)amino]-3-phenylquinoxalin-2-yl}pentanoic Acid (12). 1H NMR (600 MHz, CDCl3) δ 8.01 (d, J=8.9 Hz, 1H), 7.83 (d, J=2.4 Hz, 1H), 7.56 (d, J=6.9 Hz, 2H), 7.44-7.50 (m, 3H), 7.42 (dd, J=9.0, 2.4 Hz, 1H), 7.20-7.36 (m, 6H), 4.44 (s, 2H), 3.02 (dd, J=17.3, 9.5 Hz, 2H), 2.22-2.35 (m, 2H), 1.72-1.83 (m, 2H), 1.56-1.67 (m, 2H). MS (M+H)=476.
Example 13




Step 1
7-Bromo-3-phenylpyrido[2,3-b]pyrazin-2(1H)-one




To a solution of benzoylformic acid (4.0 g, 27 mmol) in CH2Cl2 was added catalytic DMF (0.05 eq), followed by the addition of oxalyl chloride (1.0 eq) slowly. The reaction mixture was stirred for 4 h, and then concentrated under reduced pressure. The residue was taken up in THF (20 mL) and the resulting solution was added slowly to a mixture of 5-bromopyridine-2,3-diamine (13A, 1.0 eq) and Et3N (1.0 eq) in THF (100 mL) at 0° C. The reaction mixture was kept stirring at 0° C. for 30 min, then RT for 2 h, then heated at reflux overnight. The reaction mixture was cooled to RT, diluted with aqueous NaHCO3 and CH2Cl2. The organic layer was separated, and the aqueous layer was extracted with CH2Cl2. The combined organics were washed with H2O, brine, dried over MgSO4, and concentrated. To the resultant viscous dark oil was added CH2Cl2 ( ̃80 mL), stirred vigorously for 15 min. The mixture was filtered and the brown solid obtained was washed with CH2Cl2 to give 13B, which was contaminated by some minor impurities. This material was used for next step without purification. 1H-NMR (600 MHz, DMSO-d6) δ 12.7 (s, 1H), 8.58 (s, 1H), 8.28-8.30 (m, 2H), 7.83 (s, 1H), 7.45-7.55 (m, 3H). MS (M+H)=302.
Step 2
7-Bromo-2-chloro-3-phenylpyrido[2,3-b]pyrazine




To a flask containing 7-bromo-3-phenylpyrido[2,3-b]pyrazin-2(1H)-one (13B, 1.2 g, 4.0 mmol) was added phosphorus oxychloride (20 eq), and the mixture was heated at reflux for 2 h. The reaction mixture was then cooled to RT, slowly poured a beaker containing aqueous NaHCO3 solution, and extracted with CH2Cl2. The combined organics were washed with H2O, brine, dried over MgSO4. Concentration and purification by flash chromatography 13C which was contaminated by some minor impurities. The pure title compound was obtained through recrystallization from EtOAc. MS (M+H)=320.
Step 3
Methyl 2-Chloro-3-phenylpyrido[2,3-b]pyrazine-7-carboxylate




To a flask containing a solution of 7-bromo-2-chloro-3-phenylpyrido[2,3-b]pyrazine (13C, 1 g, 3.1 mmol) in dioxane (20 mL) was added Et3N (10 mL) and MeOH (10 eq). The mixture was degassed by bubbling N2 for 10 min. Then Pd(OAc)2 (0.1 eq) and xanphos (0.2 eq) were added, and a balloon filled with CO (g) was connected to the reaction flask via three-way adapter. The mixture was vacuumed and back-filled with CO(g) three times from CO(g) balloon. The mixture was then heated at 70° C. overnight. The reaction mixture was cooled to RT, diluted with CH2Cl2, and aqueous NaHCO3. The organic layer was separated, and the aqueous layer was extracted with CH2Cl2. The combined organics were washed with brine, dried over MgSO4. Concentration and flash chromatography afforded 13D. 1H-NMR (600 MHz, CDCl3) δ 9.70 (d, J=2.4 Hz, 1H), 9.15 (d, J=2.4 Hz, 1H), 8.01-8.03 (m, 2H), 7.54-7.60 (m, 3H), 4.07 (s, 3H). MS (M+H)=300.
Step 4
Methyl 2-(5-tert-Butoxy-5-oxopentyl)-3-phenylpyrido[2,3-b]pyrazine-7-carboxylate




To a solution of tert-butyl pent-4-enoate (0.47 g, 3.0 mmol) in 3 mL THF at 0° C. was added 0.5 M 9-BBN in THF (1.05 eq) dropwise, and the reaction mixture was stirred at 0° C. for 30 min, then RT for 3 h.
To a microwave reaction vessel containing ethyl 2-chloro-3-phenylpyrido[2,3-b]pyrazine-7-carboxylate (13D, 0.28 g, 0.95 mmol) in THF (0.3 M) was added K3PO4.H2O (2.5 eq), and a solution of alkyl 9-BBN reagent from pot above (1.8 eq). The mixture was degassed by bubbling argon for 10 min. Then Pd(OAc)2 (0.08 eq) and S-Phos (0.16 eq) was added, and the mixture was heated at 70° C. for 1.5 h. The reaction mixture was cooled to RT, diluted with aq. NaHCO3, and extracted with EtOAc. The combined organics were washed with brine, dried over MgSO4. Concentration and flash chromatography gave 13E. MS (M+H)=422.
Step 5
5-[7-(Methoxycarbonyl)-3-phenylpyrido[2,3-b]pyrazin-2-yl]pentanoic Acid




To a solution of methyl 2-(5-tert-butoxy-5-oxopentyl)-3-phenylpyrido[2,3-b]pyrazine-7-carboxylate (13E, 35 mg, 0.08 mmol) in CH2Cl2 (2.0 mL) was added TFA (0.5 mL). The reaction mixture was stirred at RT for 4 h. The mixture was concentrated, and minimal amount of THF was added. The title compound precipitated 13 and was collected through filtration. MS (M+H)=366.
Example 14




Step 1
Methyl 2-(5-tert-Butoxy-5-oxopentyl)-3-phenylpyrido[2,3-b]pyrazine-7-carboxylate




To a solution of methyl 2-(5-tert-butoxy-5-oxopentyl)-3-phenylpyrido[2,3-b]pyrazine-7-carboxylate (13E, 0.25 g, 0.60 mmol) in a mixture of THF/H2O (3.0 mL/I 5 mL) was added LiOH (2.0 eq), and the mixture was stirred at RT for 3 h. The mixture was then diluted with EtOAc and H2O, and acidified with 1N HCl to pH=3 ̃4. The organic layer was separated, washed with brine, dried over MgSO4. Concentration afforded the desired carboxylic acid 14A, which was used for next step without purification.
Step 2
tert-Butyl 5-(3-Phenyl-7-{[4-(trifluoromethyl)benzyl]carbamoyl}pyrido[2,3-b]pyrazin-2-yl)pentanoate




To a flask containing the carboxylic acid 14A (40 mg, 0.1 mmol) in DMF (0.05) was added amine (1.5 eq), Hunig's Base (3.0 eq), HOBT (1.7 eq), and EDC (1.3 eq). The mixture was stirred at ambient temperature overnight. The mixture was purified by flash chromatography to afford 14B. 1H-NMR (600 MHz, CDCl3) δ 9.49 (s, 1H), 8.75 (d, J=2.0 Hz, 1H), 7.46-7.63 (m, 10H), 7.24 (d, J=2.0 Hz, 1H), 4.74 (d, J=5.4 Hz, 2H), 3.08 (t, J=7.2 Hz, 2H), 2.15 (t, J=7.2 Hz, 2H), 1.75-1.80 (m, 2H), 1.54-1.58 (m, 2H). MS (M+H)=565.
Step 3
5-(3-Phenyl-7-{[(4-(trifluoromethyl)benzyl]carbamoyl}pyrido[2,3-b]pyrazin-2-yl)pentanoic Acid




To a solution of [tert-butyl 5-(3-phenyl-7-{[4-(trifluoromethyl)benzyl]-carbamoyl}pyrido[2,3-b]pyrazin-2-yl)pentanoate (14B, 41 mg, 0.07 mmol) in DCM (2 mL) at RT was added TFA (0.5 mL), and the mixture was stirred at RT for 3 h. The mixture was concentrated, and the title compound 14 precipitated out after the addition of minimal amount of Et2O, which was collected by filtration. MS (M+H)=509.
In a manner similar to that described for the synthesis of 14, the following compounds were prepared by coupling acid 14A to the appropriate amine reagent followed by a TFA deprotection:














No.
Reagent
Compound Structure
Name
M + H







14C








5-(3-phenyl-7-{[(1S)-1- phenylethyl]carbamoyl} pyrido[2,3-b]pyrazin-2- yl)pentanoic acid
455


 


14D








5-{7-[(4-chlorobenzyl)- carbamoyl]-3- phenylpyrido[2,3- b]pyrazin-2-yl} pentanoic acid
475


 


14E








5-{3-phenyl-7-[(1- phenylcyclopropyl) carbamoyl]- pyrido[2,3-b]pyrazin- 2-yl}pentanoic acid
467


 


14F








5-(3-phenyl-7-{[(1R)-1- phenylethyl]carbamoyl} pyrido[2,3-b]pyrazin-2- yl)pentanoic acid
455


 


14G








5-{3-phenyl-7-[(4- phenylpiperazin-1- yl)carbonyl]pyrido [2,3-b]pyrazin- 2-yl}pentanoic acid
496


 


14H








5-{3-phenyl-7-[(1- pyridin-4-ylethyl) carbamoyl]pyrido[2,3- b]pyrazin-2-yl} pentanoic acid
456









Example 15
In a manner similar to that described for the synthesis of 3A, the following compounds were prepared by coupling acid 6D to the appropriate amine reagent followed by a TFA deprotection:


















M +


No.
Reagent
Compound Structure
Name
H







15A








5-(3-phenyl-7-{[(1R)-1- phenylethyl]carbamoyl} quinoxalin-2-yl) pentanoic acid
454


 


15B








5-(3-phenyl-7-{[(1S)-1- phenylethyl]carbamoyl} quinoxalin-2-yl) pentanoic acid
454


 


15C








5-(3-phenyl-7-{[4- (trifluoromethyl)benzyl] carbamoyl}quinoxalin- 2-yl)pentanoic acid
508


 


15D








5-(7-{[(1R)-1-(4- fluorophenyl)ethyl] carbamoyl}-3-phenyl- quinoxalin-2- yl)pentanoic acid
472


 


15E








5-(7-{[(1S)-1-(4- fluorophenyl)ethyl] carbamoyl}-3- phenylquinoxalin-2- yl)pentanoic acid
472


 


15F








5-(3-phenyl-7-{[(1S)- 2,2,2-trifluoro-1- phenylethyl] carbamoyl} quinoxalin-2-yl) pentanoic acid
508


 


15G








5-(3-phenyl-7-{[(1R)- 2,2,2-trifluoro-1- phenylethyl]carbamoyl} quinoxalin-2-yl) pentanoic acid
508


 


15H








5-[7-(2,7-diazaspiro- [3.5]non-7-ylcarbonyl)- 3-phenylquinoxalin-2- yl]pentanoic acid
459


 


15i








5-[7-(2,7-diazaspiro- [3.5]non-2-ylcarbonyl)- 3-phenylquinoxalin-2- yl]pentanoic acid
459


 


15J








5-{3-phenyl-7-[(3- phenylpiperazin-1- yl)carbonyl]quinoxalin- 2-yl}pentanoic acid
495


 


15K








5-{7-[(3- benzylpiperazin- 1-yl)carbonyl]-3- phenyl-quinoxalin- 2-yl}pentanoic acid
509


 


15L








5-{7-[(4-ethylpiperazin- 1-yl)carbonyl]-3- phenylquinoxalin-2- yl}pentanoic acid
447


 


15M








5-(7-{[4-(2-methoxy- ethyl)piperazin-1-yl]- carbonyl}-3-phenyl- quinoxalin-2-yl) pentanoic acid
477


 


15N








5-(7-{[3-(4-methoxy- phenyl)pyrrolidin-1-yl]- carbonyl}-3-phenyl- quinoxalin-2-yl) pentanoic acid
510


 


15o








5-(3-phenyl-7-{[4- (phenylsulfonyl) piperazin-1- yl]carbonyl}quinoxalin- 2-yl)pentanoic acid
559


 


15P








5-(7-{[3-(3-chloro- phenyl)pyrrolidin-1-yl]- carbonyl}-3-phenyl- quinoxalin-2-yl) pentanoic acid
514


 


15Q








5-(3-phenyl-7-{[2,2,2- trifluoro-1-(4-fluoro- phenyl)ethyl] carbamoyl}-quinoxalin- 2-yl)pentanoic acid
526


 


15R








5-{7-[(3- benzylpyrrolidin- 1-yl)carbonyl]-3- phenyl-quinoxalin-2- yl}pentanoic acid
494


 


15S








5-(7-{[3-(4- fluorophenyl)- pyrrolidin-1-yl] carbonyl}-3- phenylquinoxalin-2- yl)-pentanoic acid
498


 


15T








5-{7-[(4-chlorobenzyl)- carbamoyl]-3-phenyl- quinoxalin-2-yl} pentanoic acid
474


 


15U








5-{7-[(3-chlorobenzyl) carbamoyl]-3- phenylquinoxalin-2- yl}pentanoic acid
474


 


15V








5-{7-[(2-chlorobenzyl) carbamoyl]-3- phenylquinoxalin-2- yl}pentanoic acid
474


 


15X








5-{7-[(1-methyl-1- phenylethyl)carbamoyl]- 3-phenylquinoxalin-2- yl}pentanoic acid
468


 


15Y








5-{3-phenyl-7-[(3- phenylpiperidin-1- yl)carbonyl]quinoxalin- 2-yl}pentanoic acid
494


 


15Z








5-[7-(benzylcarbamoyl)- 3-phenylquinoxalin-2- yl]pentanoic acid
440


 


15AA








5-[3-phenyl-7- (phenylcarbamoyl) quinoxalin-2-yl] pentanoic acid
426


 


15AB








5-{7-[(2-fluorobenzyl) carbamoyl]-3- phenylquinoxalin-2- yl}pentanoic acid
458


 


15AC








5-{7-[(3-fluorobenzyl) carbamoyl]-3- phenylquinoxalin-2- yl}pentanoic acid
458


 


15AD








5-{7-[(4-fluorobenzyl) carbamoyl]-3- phenylquinoxalin-2- yl}pentanoic acid
458


 


15AE








5-{3-phenyl-7-[(1- phenylcyclopropyl) carbamoyl]quinoxalin-2- yl}pentanoic acid
466


 


15AF








5-{3-phenyl-7-[(pyridin- 2-ylmethyl)carbamoyl] quinoxalin-2-yl} pentanoic acid
441


 


15AG








5-{3-phenyl-7-[(pyridin- 3-ylmethyl)carbamoyl] quinoxalin-2-yl} pentanoic acid
441


 


15AH








5-{3-phenyl-7-[(pyridin- 4-ylmethyl)carbamoyl] quinoxalin-2-yl} pentanoic acid
441


 


15Ai








5-{3-phenyl-7-[(4- phenylpiperidin-1- yl)carbonyl]quinoxalin- 2-yl}pentanoic acid
494


 


15AJ








5-{3-phenyl-7-[(2- phenylpiperidin-1- yl)carbonyl]quinoxalin- 2-yl}pentanoic acid
494


 


15AK








5-{3-phenyl-7-[(3- phenylpyrrolidin-1- yl)carbonyl]quinoxalin- 2-yl}pentanoic acid
480


 


15AL








5-{3-phenyl-7-[(2- phenylpyrrolidin-1- yl)carbonyl]quinoxalin- 2-yl}pentanoic acid
480


 


15AM








5-{3-phenyl-7-[(1- phenylcyclobutyl) carbamoyl]quinoxalin- 2-yl}pentanoic acid
480


 


15AN








5-{3-phenyl-7-[(4- phenylpiperazin-1- yl)carbonyl]quinoxalin- 2-yl}pentanoic acid
495


 


15Ao








5-{7-[benzyl(methyl) carbamoyl]-3- phenylquinoxalin-2- yl}pentanoic acid
454


 


15AP








5-[7-(3,4- dihydroisoquinolin- 2(1H)-ylcarbonyl)-3- phenylquinoxalin-2- yl]pentanoic acid
466


 


15AQ








5-{7-[(2-hydroxy-2,3- dihydro-1H-inden-1- yl)carbamoyl]-3- phenylquinoxalin-2- yl}pentanoic acid
482


 


15AR








5-[3-phenyl-7-(1,2,3,4- tetrahydronaphthalen-1- ylcarbamoyl)quinoxalin- 2-yl]pentanoic acid
480


 


15AS








5-[7-(ethylcarbamoyl)- 3-phenylquinoxalin-2- yl]pentanoic acid
378


 


15AT








5-[7-(2,3-dihydro- 1H-inden- 1-ylcarbamoyl)-3- phenylquinoxalin-2- yl]pentanoic acid
466


 


15AU








5-(7-{[1-(4- methoxyphenyl)ethyl] carbamoyl}-3-phenyl- quinoxalin-2-yl) pentanoic acid
484


 


15AV








5-(3-phenyl-7-{[4- (trifluoromethoxy) benzyl]carbamoyl} quinoxalin-2- yl)pentanoic acid
524


 


15AW








5-{7-[(4-methylbenzyl) carbamoyl]- 3-phenylquinoxalin-2- yl}pentanoic acid
454


 


15AX








5-(7-{[(1R)-1-(4- chlorophenyl)ethyl] carbamoyl}-3-phenyl- quinoxalin-2- yl)pentanoic acid
488


 


15AY








5-(7-{[(1R)-1-(4- methylphenyl)ethyl] carbamoyl}-3-phenyl- quinoxalin-2- yl)pentanoic acid
468


 


15AZ








5-{7-[(4- ethylbenzyl)carbamoyl]- 3-phenylquinoxalin-2- yl}pentanoic acid
468


 


15AB








5-{7-[(4-methoxybenzyl) carbamoyl]-3- phenylquinoxalin-2- yl}pentanoic acid
470


 


15BB








5-{7-[(4-cyanobenzyl) carbamoyl]-3- phenylquinoxalin-2- yl}pentanoic acid
465


 


15BC








5-(7-{[(1S)-1-(4- methylphenyl)ethyl] carbamoyl}-3- phenylquinoxalin-2- yl)pentanoic acid
468


 


15BD








5-[3-phenyl-7-({1-[4- (trifluoromethoxy) phenyl]ethyl}carbamoyl) quinoxalin-2-yl] pentanoic acid
538


 


15BE








5-[3-phenyl-7- (pyrrolidin-1- ylcarbonyl)quinoxalin- 2-yl]pentanoic aicd
404


 


15BF








5-[3-phenyl-7- (piperidin-1-ylcarbonyl) quinoxalin-2-yl] pentanoic acid
418


 


15BG








5-[7-(methylcarbamoyl)- 3-phenylquinoxalin-2- yl]pentanoic acid
364


 


15BH








5-(7-{[1-(2- fluorophenyl)ethyl] carbamoyl}-3- phenylquinoxalin-2- yl)pentanoic acid
472


 


15Bi








5-[7-(3,4-dihydro- quinolin-1(2H)- ylcarbonyl)-3- phenylquinoxalin-2- yl]pentanoic acid
466


 


15BJ








5-{7- [methoxy(methyl) carbamoyl]-3- phenylquinoxalin-2- yl}pentanoic acid
394


 


15BK








5-[7-(2,3-dihydro-1H- inden-2-ylcarbamoyl)-3- phenylquinoxalin-2- yl]pentanoic acid
466


 


15BL








5-[3-phenyl-7-({(1R)-1- [2-(trifluoromethyl) phenyl]ethyl}carbamoyl) quinoxalin-2- yl]pentanoic acid
522


 


15BM








5-(7-{[(1S)-1-(4- chlorophenyl)ethyl] carbamoyl}-3- phenylquinoxalin-2- yl)pentanoic acid
488


 


15BN








5-(7-{[3-(2- oxopyrrolidin-1-yl) propyl]carbamoyl}-3- phenylquinoxalin-2- yl)pentanoic acid
475


 


15Bo








5-{7-[methyl (tetrahydrofuran-3-yl) carbamoyl]-3-phenyl quinoxalin-2-yl} pentanoic acid
434


 


15BQ








5-(7-{[4-(4-methyl-1H- 1,2-3-triazol-1-yl) piperidin-1-yl] carbonyl}-3-phenyl quinoxalin-2-yl) pentanoic acid
499


 


15BR








5-(7-{[4-(5-methyl-1H- 1,2,3-triazol-1-yl) piperidin-1-yl] carbonyl}-3- phenylquinoxalin-2- yl)pentanoic acid
499


 


15BS








5-{3-phenyl-7-[(4- pyrazin-2-ylpiperidin-1- yl)carbonyl]quinoxalin- 2-yl}pentanoic acid
496


 


15BT








5-(7-{[(4-chloropyridin- 2-yl)methyl]carbamoyl}- 3-phenylquinoxalin-2- yl)pentanoic acid
475


 


15BU








5-(7-{[(6-chloropyridin- 2-yl)methyl]carbamoyl}- 3-phenylquinoxalin-2- yl)pentanoic acid
475









Preparative Example 16




In a manner similar to that previously, methyl 3,4-diaminobenzoate was 2-(4-fluorophenyl)-2-oxoacetic acid (prepared from ethyl 2-(4-chlorophenyl)-2-oxoacetate as described in Example 2) to provide 16A (MS: M+H=315).
A mixture of 16A (13.43 g, 42.67 mmol), SOCK (70 mL, 1000 mmol), and DMF (0.4 mL, 5 mmol) was stirred at 80° C. for 4 h and then at 40° C. overnight.
The excess SOCl2 was removed. The resulting residue was stirred with cold water for 2 h. The solid was then filtered and dried overnight in vacuo to give methyl 3-chloro-2-(4-chlorophenyl)quinoxaline-6-carboxylate (16B, 12.94 g; Yield=91.04%). LCMS (M+H)=333.
In a manner similar to that previously described, 16B was sequentially coupled with compound 1, hydrolyzed with LiOH (to afford 16D), coupled with 4-chlorobenzylamine, and deprotected with TFA to provide the title compound 16. LCMS (M+H)=508




In a similar manner, the following compounds were prepared by coupling acid 16D to the appropriate amine reagent followed by a TFA deprotection:













No.
Compound
Name
M + H







16F




3-(4-chlorophenyl)-7-[[[(3- chlorophenyl)methyl]amino] carbonyl]-2- quinoxalinepentanoic acid
508


 


16G




3-(4-chlorophenyl)-7- [[(phenylmethyl)amino]car- bonyl]-2- quinoxalinepentanoic acid
474


 


16H




3-(4-chlorophenyl)-7-[(3- phenyl-1- pyrrolidinyl)carbonyl]-2- quinoxalinepentanoic acid
514


 


16i




3-(4-chlorophenyl)-7- [[(2,2,2-trifluoro-1(s)- phenylethyl)amino]carbonyl]- 2-quinoxalinepentanoic acid
542


 


16J




3-(4-chlorophenyl)-7-[(4- phenyl-1- piperidinyl)carbonyl]-2- quinoxalinepentanoic acid
528


 


16K




3-(4-chlorophenyl)-7-[[[(4- cyanophenyl)methyl]amino] carbonyl]-2- quinoxalinepentanoic acid
499


 


16L




3-(4-chlorophenyl)-7-[(3(R)- phenyl-1- pyrrolidinyl)carbonyl]-2- quinoxalinepentanoic acid
514


 


16M




3-(4-chlorophenyl)-7-[(3(S)- phenyl-1- pyrrolidinyl)carbonyl]-2- quinoxalinepentanoic acid
514


 


16N




3-(4-chlorophenyl)-7-[(3(R)- phenyl-1- piperidinyl)carbonyl]-2- quinoxalinepentanoic acid
528


 


16o




3-(4-chlorophenyl)-7-[(3(S)- phenyl-1- piperidinyl)carbonyl]-2- quinoxalinepentanoic acid
528


 


16P




3-(4-chlorophenyl)-7-[[[[4- (trifluoromethoxy)phenyl] methyl]amino]carbonyl]-2- quinoxalinepentanoic acid
558


 


16Q




3-(4-chlorophenyl)-7-[[[(3,5- dichlorophenyl)methyl]amino] carbonyl]-2- quinoxalinepentanoic acid
542


 


16R




3-(4-chlorophenyl)-7-[[[(4- fluorophenyl)methyl]amino] carbonyl]-2- quinoxalinepentanoic acid
492


 


16S




3-(4-chlorophenyl)-7- [[[1(R)-(4-fluorophenyl) ethyl]amino]carbonyl]-2- quinoxalinepentanoic acid
506


 


16T




3-(4-chlorophenyl)-7- [[[1(S)-(4-fluorophenyl) ethyl]amino]carbonyl]-2- quinoxalinepentanoic acid
506


 


16U




3-(4-chlorophenyl)-7-[[[[4- (trifluoromethyl)phenyl]methyl] amino]carbonyl]-2- quinoxalinepentanoic acid
542


 


16V




3-(4-chlorophenyl)-7- [[[1(R)-(4-chlorophenyl)- 2,2,2-trifluoroethyl] amino]carbonyl]-2- quinoxalinepentanoic acid
576


 


16W




3-(4-chlorophenyl)-7- [[[(1(S)-(4-chlorophenyl)- 2,2,2-trifluoro-ethyl]amino] carbonyl]-2- quinoxalinepentanoic acid
576


 


16X




3-(4-chlorophenyl)-7- [[[2,2,2-trifluoro-1(R)-(4- fluorophenyl)ethyl]amino] carbonyl]-2- quinoxalinepentanoic acid
560


 


16Y




3-(4-chlorophenyl)-7- [[[2,2,2-trifluoro-1(S)-(4- fluorophenyl)ethyl]amino] carbonyl]-2- quinoxalinepentanoic acid
560


 


16Z




3-(4-chlorophenyl)-7- [[[1(S)-(2-pyridinyl)ethyl] amino]carbonyl]-2- quinoxalinepentanoic acid
489


 


16AA




3-(4-chlorophenyl)-7- [[[1(S)-(2-pyridinyl)ethyl] amino]carbonyl]-2- quinoxalinepentanoic acid
489


 


16AB




3-(4-chlorophenyl)-7-[[[[3- (trifluoromethyl)phenyl] methyl]amino]carbonyl]-2- quinoxalinepentanoic acid
542


 


16AC




3-(4-chlorophenyl)-7-[[[[3- (trifluoromethoxy)phenyl] methyl]amino]carbonyl]-2- quinoxalinepentanoic acid
558


 


16AD




3-(4-chlorophenyl)-7- [[[(R)-(4- chlorophenyl)ethyl]amino] carbonyl]-2- quinoxalinepentanoic acid
522


 


16AE




3-(4-chlorophenyl)-7- [[[1(S)-(4- chlorophenyl)ethyl]amino] carbonyl]-2- quinoxalinepentanoic acid
522


 


16AF




3-(4-chlorophenyl)-7-[(3- phenyl-1- azetidinyl)carbonyl]- 2- quinoxalinepentanoic acid
500


 


16AG




3-(4-chlorophenyl)-7-[(4- phenyl-1- piperazinyl)carbonyl]-2- quinoxalinepentanoic acid
529


 


16AH




3-(4-chlorophenyl)-7- [[(2,2,2-trifluoro-1(R)- phenylethyl)amino]carbonyl]- 2-quinoxalinepentanoic acid
542


 


16Ai




3-(4-chlorophenyl)-7- [[(1,2,3,4-tetrahydro-1- naphthalenyl)amino]carbonyl]- 2-quinoxalinepentanoic acid
514


 


16AJ




3-(4-chlorophenyl)-7-[(3,6- dihydro-4-phenyl-1(2H)- pyridinyl)carbonyl]-2- quinoxalinepentanoic acid
526


 


16AK




3-(4-chlorophenyl)-7-[(4- cyano-4-phenyl-1- piperidinyl)carbonyl]-2- quinoxalinepentanoic acid
553


 


16AL




3-(4-chlorophenyl)-7- [[(1(R)- phenylethyl)amino]carbonyl]- 2-quinoxalinepentanoic acid
488


 


16AM




3-(4-chlorophenyl)-7- [[(1-(S)- phenylethyl)amino]carbonyl]- 2-quinoxalinepentanoic acid
488


 


16AN




3-(4-chlorophenyl)-7- [[(1,2,3,4-tetrahydro-1(S)- naphthalenyl)amino]carbonyl]- 2-quinoxalinepentanoic acid
514


 


16Ao




3-(4-chlorophenyl)-7-[[(2,3- dihydro-1H-inden-1(R)- yl)amino]carbonyl]-2- quinoxalinepentanoic acid
474


 


16AP




3-(4-chlorophenyl)-7-[[(2,3- dihydro-1H-inden-1(S)- yl)amino]carbonyl]-2- quinoxalinepentanoic acid
500


 


16AQ




3-(4-chlorophenyl)-7-[[[(4- methylphenyl)methyl]amino] carbonyl]-2- quinoxalinepentanoic acid
488


 


16AR




3-(4-chlorophenyl)-7-[[(3- hydroxy-1(S)- phenylpropyl)amino]carbonyl]- 2-quinoxalinepentanoic acid
518


 


16AS




3-(4-ch1orophenyl)-7-[[(2,3- dihydro-1H-inden-2- yl)amino]carbonyl]-2- quinoxalinepentanoic acid
500









Example 17




in a manner similar to that previously, methyl 3-chloro-2-(4-chlorophenyl)quinoxaline-6-carboxylate (16B) was coupled with tert-butyl 3-methylpent-4-enoate (prepared from 3-methyl-4-pentenoic acid, TFAA, and tBuOH in a manner similar to that described for compound 1) to provide 17A (LCMS: M+H=469), which was subsequently hydrolyzed with LiOH (to afford 17B, LCMS: M+H=455), coupled with 4-phenylpiperidine and deprotected with TFA to provide 3-(4-chlorophenyl)-beta-methyl-7-[(4-phenyl-1-piperidinyl)carbonyl]-2-quinoxalinepentanoic acid 17. LCMS (M+H)=542.




in a similar manner, the following compounds were prepared by coupling acid 17B to the appropriate amine reagent followed by a TFA deprotection:













No.
Compound
Name
M + H







17D




3-(4-chlorophenyl)-7-[[[1(S)- (4-chlorophenyl)ethyl] aminocarbonyl]-beta- methyl-2-quinoxaline pentanoic acid
536


 


17E




3-(4-chlorophenyl)-7- [[[1(R)-(4- chlorophenyl)ethyl]amino] carbonyl]-beta-methyl-2- quinoxalinepentanoic acid
536


 


17F




3-(4-chlorophenyl)-beta- methyl-7-[(3-phenyl-1- azetidinyl)carbonyl]-2- quinoxalinepentanoic acid
514


 


17G




3-(4-chlorophenyl)-beta- methyl-7-[[[2,2,2-trifluoro- 1(R)-(4-fluorophenyl)ethyl] aminocarbonyl]-2- quinoxalinepentanoic acid
574


 


17H




3-(4-chlorophenyl)-7-[[[(4- methoxyphenyl)methyl] aminocarbonyl]-beta- methyl-2-quinoxaline pentanoic acid
518


 


17i




3-(4-chlorophenyl)-beta- methyl-7-[(3(S)-phenyl-1- piperidinyl)carbonyl]-2- quinoxalinepentanoic acid
542


 


17J




3-(4-chlorophenyl)-beta- methyl-7-[(3(R)-phenyl-1- piperidinyl)carbonyl]-2- quinoxalinepentanoic acid
542


 


17K




3-(4-chlorophenyl)-beta- methyl-7-[(3(S)-phenyl-1- pyrrolidinyl)carbonyl]-2- quinoxalinepentanoic acid
528


 


17L




3-(4-chlorophenyl)-beta- methyl-7-[(3(R)-phenyl-1- pyrrolidinyl)carbonyl]-2- quinoxalinepentanoic acid
528


 


17M




3-(4-chlorophenyl)-beta- methyl-7-[[[2,2,2-trifluoro- (1S)-phenylethyl]amino] carbonyl]-2-quinoxaline pentanoic acid
556


 


17N




3-(4-chlorophenyl)-beta- methyl-7-[[(2,2,2-trifluoro- 1(R)- phenylethyl)amino]carbonyl]- 2-quinoxalinepentanoic acid
556


 


17o




3-(4-chlorophenyl)-7- [[[1(R)-(4-methoxyphenyl) ethyl]amino]carbonyl]-beta- methyl-2-quinoxaline pentanoic acid
532


 


17P




3-(4-chlorophenyl)-7-[[[1(S)- (4-methoxyphenyl)ethyl] amino]carbonyl]-beta- methyl-2-quinoxaline pentanoic acid
532









In a similar manner to that described above, the following compounds were prepared from methyl 3-chloro-2-(4-(trifluoromethyl)phenyl)quinoxaline-6-carboxylate (2i) and tert-butyl 3-methylpent-4-enoate, followed by sequential hydrolysis with LiOH, coupling with an appropriate amine and deprotection with TFA.













No
Compound
Name
M + H







17Q




7-[[[(3-chlorophenyl)methyl] amino]carbonyl]-beta- methyl-3-[4-(trifluoro methyl)phenyl]-2- quinoxalinepentanoic acid
556


 


17R




beta-methyl-7-[(4-phenyl-1- piperidinyl)carbonyl]-3-+4- (trifluoromethyl)phenyl]-2- quinolinepentanoic acid
576


 


17S




beta-methyl-3-[4- (trifluoromethyl)phenyl]-7- [(2,2,2-trifluoro-1(S)- phenylethyl)amino]carbonyl]- 2-quinoxalinepentanoic acid
590


 


17T




7-[[(2,3-dihydro-1H-inden-2- yl)amino]carbonyl]-beta- methyl-3-[4- (trifluoromethyl)phenyl]-2- quinoxalinepentanoic acid
548


 


17U




beta-methyl-7-[(3(R)-phenyl- 1-pyrrolidinyl)carbonyl]-3- [4-(trifluoromethyl)phenyl]- 2-quinoxalinepentanoic acid
562


 


17V




beta-methyl-7-[(3(S)-phenyl- 1-pyrrolidinyl)carbonyl]-3- [4-(trifluoromethyl)phenyl]- 2-quinoxalinepentanoic acid
562


 


17W




beta-methyl-7-[(3-phenyl-1- azetidinyl)carbonyl]-3-[4- (trifluoromethyl)phenyl]-2- quinoxalinepentanoic acid
548


 


17X




7-[[[(4- chlorophenyl)methyl]amino] carbonyl]-beta-methyl-3-[4- (trifluoromethyl)phenyl]-2- quinoxalinepentanoic acid
556


 


17Y




beta-methyl-7-[[[[4- (trifluoromethoxy)phenyl] methyl]amino]carbonyl]-3-[4- (trifluoromethyl)phenyl]-2- quinoxalinepentanoic acid
606


 


17Z




7-[[[(4- cyanophenyl)methyl]amino] carbonyl]-beta-methyl-3-[4- (trifluoromethyl)phenyl]-2- quinoxalinepentanoic acid
547









Example 18




In a manner similar to that previously, methyl 3-chloro-2-(4-(trifluoromethyl)phenyl)quinoxaline-6-carboxylate (2i) was coupled tert-butyl 2-methylpent-4-enoate (prepared from 2-methylpent-4-enoic acid, TFAA, and tBuOH in a manner similar to that described for compound 1) to provide 18A, which was subsequently hydrolyzed with LiOH, coupled with 4-phenylpiperidine and deprotected with TFA to provide alpha-methyl-7-[(4-phenyl-1-piperidinyl)carbonyl]-3-[4-(trifluoromethyl)phenyl]-2-quinoxalinepentanoic acid 18. LCMS (M+H)=576.




In a similar manner, the following compounds were prepared by coupling acid 18B to the appropriate amine reagent followed by a TFA deprotection:













No.
Compound
Name
M + H







18D




7-[[[(3-chlorophenyl) methyl]amino]carbonyl]- alpha-nethyl-3-[4-(trifluoro methyl)phenyl]-2- quinoxalinepentanoic acid
556


 


18E




alpha-methyl-7-[(3(S)- phenyl-1-pyrrolidinyl) carbonyl]-3-[4- (trifluoromethyl)phenyl]-2- quinoxalinepentanoic acid
562


 


18F




alpha-methyl-7-[(3(R)- phenyl-1- pyrrolidinyl)carbonyl]-3-[4- (trifluoromethyl)phenyl]-2- quinoxalinepentanoic acid
562


 


18G




7-[[(2,3-dihydro-1H-inden-2- yl)amino]carbonyl]-alpha- methyl-3-[4- (trifluoromethyl)phenyl]-2- quinoxalinepentanoic acid
548


 


18H




alpha-methyl-3-[4- (trifluoromethyl)phenyl]-7- [[(2,2,2-trifluoro-1(S)- phenylethyl)amino]carbonyl]- 2-quinoxalinepentanoic acid
590









Example 19




Steps 1-2
tert-Butyl 2-(3-Vinylphenyl)acetate




In a manner similar to that described for compound 1,2-(3-bromophenyl)acetic acid was reacted with TFAA and tBuOH to provide tert-butyl 2-(3-bromophenyl)acetate (19A).
A solution of 19A (600 mg, 2.21 mmol), potassium vinyltrifluoroborate (296 mg, 2.21 mmol), Pd(dppf)2Cl2.CH2Cl2 (180 mg, 0.221 mmol), and cesium carbonate (2.16 g, 6.63 mmol) in water (1 mL) and 1,4-dioxane (9 mL) under nitrogen was stirred at 90° C. for 18 h. After this time, the reaction was cooled to RT and diluted with water and DCM. The aqueous layer was separated and extracted with DCM. The combined organics were washed with brine, dried (Na2SO4), filtered, and concentrated. The residue was purified by flash chromatography (0% to 20% EtOAc/heptane) to yield 19B (268 mg, yield=56%): 1H NMR (500 MHz, CDCl3) δ 7.32-7.24 (m, 3H), 7.18-7.15 (m, 1H), 6.70 (dd, J=23.5, 14.5 Hz, 1H), 5.75 (d, J=23.5 Hz, 1H), 5.24 (d, J=14.5 Hz, 1H), 3.52 (s, 2H), 1.44 (s, 9H).
Steps 3-6
2-(3-(2-(3-(4-chlorophenyl)-7-(4-phenylpiperidine-1-carbonyl)quinoxalin-2-yl)ethyl)phenyl)acetic acid




In a manner similar to that previously, tert-butyl 2-(3-vinylphenyl)acetate (19B) was sequentially coupled with methyl 3-chloro-2-(4-chlorophenyl)quinoxaline-6-carboxylate (16B) and hydrolyzed with LiOH. The resulting acid 19C (3-(3-(2-tert-butoxy-2-oxoethyl)phenethyl)-2-(4-chlorophenyl)quinoxaline-6-carboxylic acid) was coupled with 4-phenylpiperidine and deprotected with TFA to provide -[2-[3-(4-chlorophenyl)-7-[(4-phenyl-1-piperidinyl)carbonyl]-2-quinoxalinyl]ethyl]benzeneacetic acid 19. LCMS (M+H)=590.
In a similar manner, the following compounds were prepared by coupling acid 19C to the appropriate amine reagent followed by a TFA deprotection:













No.
Compound
Name
M + H







19F




3-[2-[3-(4- chlorophenyl)-7-[[[(4- cyanophenyl)methyl] amino]carbonyl]-2- quinoxalinyl]ethyl] benzeneacetic acid
561


 


19G




3-[2-[3-(4- chlorophenyl)-7-[[[1(R)- (4-fluorophenyl) ethyl]amino]carbonyl]- 2-quinaxalinyl]ethyl] benzeneacetic acid
568


 


19H




3-[2-[3-(4- chlorophenyl)-7-[[[1(S)- (4-fluorophenyl)ethyl] amino]carbonyl]-2- quinoxalinyl]ethyl] benzeneacetic acid
568


 


19i




3-[2-[3-(4- chlorophenyl)-7-[[[(3- chlorophenyl)methyl] amino]carbonyl]-2- quinoxalinyl]ethyl] benzeneacetic acid
570


 


19J




3-[2-[3-(4- chlorophenyl)-7- [[(2,2,2-trifluoro-1(S)- phenylethyl)amino] carbonyl]- 2-quinoxalinyl] ethyl]benzeneacetic acid
604


 


19K




3-[2-[3-(4- chlorophenyl)-7-[(3- phenyl-1- azetidinyl)carbonyl]-2- quinoxalinyl]ethyl] benzeneacetic acid
562









Example 20




Step 1
Ethyl 2-vinylcyclopropanecarboxylate




A mixture of methyltriphenyl-phosphonium bromide (60.3 g, 168.8 mmol, 2.4 eq) in 500 mL of THF was cooled down to ° C. and treated slowly with KOtBu (18.9 g, 168.8 mmol, 2.4 eq). The reaction was stirred at ° C. for 30 min, treated dropwise with ethyl 2-fomyl-1-cyclopropanecarboxylate (10 g, 70.34 mmol, 1.0 eq) in 200 mL THF, and then stirred for 2 h at RT. The mixture was then cooled to ° C., treated with water and extracted with ether (2×). The organic extracts were dried over Na2SO4, filtered and concentrated. Chromatography (*0 to 25% ether in pentane) afforded the olefin 20A (8.15 g).
Step 2
(2-Vinylcyclopropyl)methanol




A solution of ethyl 2-vinylcyclopropanecarboxylate (20A, 4.26 g, 30.4 mmol, 1.0 eq) in THF (100 mL, anhydrous) was cooled to 0° C. and treated slowly with LAH (1.60 g, 38.0 mmol, 1.25 eq). The reaction was then slowly warmed to RT, stirred overnight, and then quenched with water. The precipitate was filtered and washed with THF. Chromatography of the filtrate (0 to 60% ether/hexanes) gave the alcohol 20B (2.0 g).
Step 3
Methyl 3-(2-(2-(hydroxymethyl)cyclopropyl)ethyl)-2-phenylquinoxaline-6-carboxylate




To a solution of (2-vinylcyclopropyl)methanol (20B, 0.5 g, 5.10 mmol, 2.0 eq) in anhydrous THF (20 mL) was added 9-BBN (0.5 M in THF, 20 mL, 10.2 mmol, 4 eq) at 0° C. under nitrogen. The reaction was stirred at 0° C. for 30 min and warmed to RT for 4 h. methyl 3-chloro-2-phenylquinoxaline-6-carboxylate 6B (0.76 g, 2.55 mmol, 1.0 eq), Pd(dppf)Cl2.CH2Cl2 (310 mg, 0.380 mmol, 0.15 eq), and K3PO4 (1.62 g, 7.65 mmol, 3.0 eq) were added. The suspension was degassed (3× vacuum/nitrogen) and heated at 68° C. overnight. The reaction was then cooled to RT and filtered. The filtrate was diluted with DCM and water. The aqueous layer was separated and extracted with DCM. The combined organics were dried (Na2SO4), filtered, and concentrated. The residue was purified by column chromatography (20%-50% EtOAc/hexane) to yield 20C (0.84 g). Further purification of the mixture (SFC chiral chromatography) provided the 4 stereoisomers, each in high purity.
Steps 4-6
2-(2-(3-phenyl-7-(4-phenylpiperidine-1-carbonyl)quinoxaline-2-yl)ethyl)cyclopropanecarboxylic acid




In a manner similar to that previously, each pure stereoisomer of 20C was individually hydrolyzed with LiOH and coupled with 4-phenylpiperidine to provide (3-(2-(2-(hydroxymethyl)cyclopropyl)ethyl)-2-phenylquinoxalin-6-yl)(4-phenylpiperidin-1-yl)methanone (20E). The first stereoisomer was then subjected to the following oxidation:
A mixture of the alcohol 20E (103 mg, 0.21 mmol, 1.0 eq), sodium periodate (135 mg, 0.629 mmol, 3.0 eq) and ruthenium chloride trihydrate (3 mg, 0.011 mmol, 0.05 eq) were combined in 3 mL CCl4-4 mL H2O-3 mL CH3CN. The mixture was stirred at RT overnight, quenched with 10 mL sat. aq. NH4Cl and filtered. The precipitate was filtered and washed with EtOAc. The aqueous layer was extracted with EtOAc (1×). The combined organic layers were dried (Na2SO4), filtered, and concentrated. Chromatography (0 to 10% MeOH/CH2Cl2) provided the title compound 20 (74 mg, LCMS: M+H 506).
The other three stereoisomers of 20E were similarly subjected to this oxidation to provide 20F (LCMS: M+H 506), 20G (LCMS: M+H 506), and 20H (LCMS: M+H 506).
Example 21




Steps 1-2
(R)-5-(3-(4-fluorophenyl)-7-(methyl(1-phenylethyl)carbamoyl)quinoxalin-2-yl)pentanoic acid




A solution of (R)-tert-butyl 5-(3-(4-fluorophenyl)-7-(1-phenylethyl-carbamoyl)quinoxalin-2-yl)pentanoate (21A, 62 mg, 0.118 mmol; derived from the coupling of 5D and (R)-1-phenylethanamine as previously described) in 5 DMF (5 mL) was cooled to 0° C. and treated with NaH (60%, 7 mg, 0.176 mmol, 1.5 eq). After 20 min at 0° C., MeI (15 μL) was added and the reaction was warmed to RT for 2 h. The reaction was concentrated and then diluted with CH2Cl2 and H2O. The H2O layer was extracted with CH2Cl2 (1×). The combined organic layers were dried (Na2SO4), filtered, and concentrated. Chromatography (0 to 50% EtOAc/Hexane) provided the (R)-tert-butyl 5-(3-(4-fluorophenyl)-7-(methyl(1-phenylethyl)carbamoyl)-quinoxalin-2-yl)pentanoate (21B, 46 mg).
In a manner similar to that previously described, 21B was deprotected with TFA to provide 21. LCMS (M+H)=486.
The enantiomer -(4-fluorophenyl)-7-[[methyl(1(S)-phenylethyl)amino]carbonyl]-2-quinoxalinepentanoic acid 21C was synthesized in a similar fashion. LCMS (M+H)=486.




Example 22




Step 1
Methyl 3-(3-cyanopropyl)-2-phenylquinoxaline-6-carboxylate




In a manner similar to that described in Example 2, the following reaction was completed: To a solution of 3-butenenitrile in THF cooled to 0° C. was added 9-BBN. The reaction was stirred at 0° C. for 30 min and at RT for 3 h. To this solution was added methyl 3-chloro-2-phenylquinoxaline-6-carboxylate (6B), Pd(dppf)Cl2.CH2Cl2 and K3PO4 The resulting solution was degassed and stirred at 60° C. for 16 h. The filtrate was diluted with DCM and water. The organic layer was removed and the aqueous phase was extracted with DCM (2×). The combined organics were dried (Na2SO4), filtered, and concentrated. The residue was purified by flash chromatography to provide 22A.
Steps 2-4
N-(3-chlorobenzyl)-3-(3-cyanopropyl)-2-phenylquinoxaline-6-carboxamide




In a manner similar to that described previously (e.g., Examples 2-3), 22A was hydrolyzed with LiOH and then treated with 3-chlorobenzylamine, DIPEA, and Bop-Cl to provide 22C.
To a solution of the nitrile 22C and trimethylsilylazide in toluene was added dibutyltinoxide, and the reaction mixture was heated at 110 degree overnight until the nitrile was consumed. The mixture was concentrated down, dissolved in methanol and reconcentrated, then partitioned between EtOAc (20 mL) and 10% NaHCO3 solution (20 mL). The organic layer was extracted with an additional portion of 10% NaHCO3 solution (20 mL). The combined aqueous extracts were acidified to pH 2 with 10% HCl and then extracted with EtOAc (2×20 mL). The combined organic extracts were dried, concentrated, and purified by reverse-phase (C18, 5-95% MeCN—H2O with 0.1% TFA) to give the 5-substituted tetrazole 22. LCMS (M+H)=484.
In a similar manner, N-[(3-chlorophenyl)methyl]-2-phenyl-3-[4-(1H-tetrazol-5-yl)butyl]-6-quinoxalinecarboxamide 22D was synthesized by using 4-pentenenitrile in Step 1, and then following the described sequence. LCMS (M+H)=498




Example 23




In a manner similar to that previously described in Examples 6 and 10, 7-[[[(3-chlorophenyl)methyl]amino]carbonyl]-3-phenyl-2-quinoxalinebutanoic acid 23 (LCMS, M+H=460) was synthesized by coupling tert-butyl 3-butenoate with 6B and then advancing the product through the described sequence.
Example 24




Step 1
Ammonium 4-amino-2-fluoro-5-nitrobenzoate




A solution of 2,4-difluoro-5-nitrobenzoic acid (24A, 10.0 g, 49.2 mmol) in 250 mL 0.5 NH3/dioxane was heated in a sealed tube at 90° C. overnight. After the reaction mixture was cooled to RT, it was filtered and the solid collected was dried over the vacuum to provide the product as yellow solid 248 (8.3 g, 77%).
Step 2
Ammonium 4,5-diamino-2-fluorobenzoate




A suspension of 24B (3.5 g, 16.1 mmol) in MeOH/CH2Cl2 (4:1 v/v, 50 mL) was added with 10% Pd/C (wet, 0.88 g) and stirred under H2 balloon overnight. The reaction mixture was then filtered and the filtrate was concentrated under vacuum to give crude product 24C (3.3 g) which was used in the next step without purification.
Step 3
3-(5-Ethoxy-5-oxopentyl)-7-fluoro-2-hydroxyquinoxaline-6-carboxylic acid




To the crude product 24C (3.3 g) in EtOH (50 mL) was added diethyl 2-oxoheptane-1,7-dicarboxylate (5.6 g, 24.2 mmol) and the mixture was heated at reflux overnight. It was then cooled to RT and acidified with 1N HCl to pH=5 and concentrated under vacuum. The resulting residue was purified by flash chromatography (CH2Cl2/MeOH) to provide 24D as gray solid (1.9 g, 35% over two steps).
Step 4
(S)-ethyl 5-(6-fluoro-3-hydroxy-7-(3-phenylpyrrolidine-1-carbonyl)-quinoxalin-2-yl)pentanoate




To a solution of 24D (509 mg, 1.4 mmol) in CH2Cl2 (10 mL) was added (S)-3-phenylpyrrolidine hydrochloride (220 mg, 1.2 mmol), HATU (912 mg, 2.4 mmol) and DIPEA (1.0 mL, 6.0 mmol) and the mixture was stirred at RT overnight. It was then diluted with CH2Cl2 and washed by H2O, brine and dried over Na2SO4, then concentrated under vacuum. The resulting residue was purified by flash chromatography (EtOAc/hexane) to provide product 24E as colorless oil (500 mg, 90%).
Step 5
(S)-ethyl 5-(6-fluoro-7-(3-phenylpyrrolidine-1-carbonyl)-3-(trifluoromethyl-sulfonyloxy)quinoxalin-2-yl)pentanoate




To a solution of 24E (700 mg, 1.5 mmol) in DMF (10 mL) was added PhNTf2 (591 mg, 1.7 mmol) and DBU (252 mg, 1.7 mmol) and the mixture was stirred at RT overnight. Then it was diluted with EtOAc and washed by H2O, brine and dried over Na2SO4, then concentrated over vacuum. The resulting residue was purified by flash chromatography (EtOAc/hexane) to provide product 24F as colorless oil (400 mg, 45%).
Step 6
(S)-ethyl 5-(3-(4-chlorophenyl)-6-fluoro-7-(3-phenylpyrrolidine-1-carbonyl)-quinoxalin-2-yl)pentanoate




To a solution of 24F (100 mg, 0.17 mmol) in dioxane (10 mL) in a sealed tube was added 4-chlorophenylboronic acid (52 mg, 0.33 mmol), K3PO4 (71 mg, 0.33 mmol) and KBr (40 mg, 0.33 mmol) and the mixture was degassed for 5 min. Then Pd(PPh3)4 (53 mg, 0.046 mmol) was added and the resulting mixture was heated at 80° C. overnight. It was then taken into EtOAc and washed by H2O, brine and dried over Na2SO4, then concentrated over vacuum. The resulting residue was purified by flash chromatography (EtOAc/hexane) to provide product 24G as colorless oil (90 mg, 95%).
Step 7
3-(4-chlorophenyl)-6-fluoro-7-[(3(S)-phenyl-1-pyrrolidinyl)carbonyl]-2-quinoxalinepentanoic acid




A mixture of 24G (90 mg, 0.16 mmol) and LiOH.H2O (10 mg, 0.24 mmol) in THF/H2O (3:1 v/v, 4 mL) was stirred at RT overnight. Then it was acidified by 1N HCl to pH=5 and concentrated under vacuum. The resulting residue was purified by reverse phase HPLC to provide product 24 as colorless oil (50 mg, 59%). MS (M+H)=532.0.
In a similar manner, the following compounds were prepared by coupling the appropriate boronic acid to 24F followed by hydrolysis.













No.
Compound
Name
M + H







24H




6-fluoro-3-(4-fluorophenyl)-7- [(3(S)-phenyl-1- pyrrolidinyl)carbonyl]-2- quinoxalinepentanoic acid
516


 


24i




6-fluoro-3-phenyl-7-[(3(S)- phenyl-1-pyrrolidinyl)carbonyl]- 2-quinoxalinepentanoic acid
498









Example 25




Step 1
3-(5-Ethoxy-5-oxopentyl)-2-oxo-1,2-dihydroquinoxaline-6-sulfonic acid




A mixture of 3,4-diaminobenzenesulfonic acid 25A (4.6 g, 24.6 mmol) and diethyl 2-oxoheptane-1,7-dicarboxylate (7.4 g, 32.0 mmol) in EtOH (100 mL) was heated at reflux overnight. Then it was concentrated under vacuum and the residue was purified by flash chromatography (CH2Cl2/MeOH) to provide 25B as brown solid (4.5 g, 52%).
Step 2
Ethyl 5-(3-oxo-7-(4-phenylpiperidin-4-ylsulfonyl)-3,4-dihydroquinoxalin-2-yl)pentanoate




A solution of 25B (1.2 g, 3.45 mmol) in pyridine (10 mL) was stirred for 30 min and then concentrated under vacuum to give pyridine salt of 25B. In a different flask, to a solution of Ph3PO (2.1 g, 7.6 mmol) in CH2Cl2 (20 mL) was added Tf2O (0.58 mL, 3.45 mmol) and the mixture was stirred for 15 min. Then the above solution was cannulated into the pyridine salt of 25B and the resulting mixture was stirred for 30 min. A solution of 4-phenylpiperidine (1.1 g, 6.9 mmol) and Et3N (4.2 mL, 15.2 mmol) in CH2Cl2 (3 mL) was then added into the above mixture slowly at 0° C. and stirred overnight. It was then diluted with CH2Cl2, washed by H2O, brine, dried over Na2SO4 and concentrated under vacuum. The resulting residue was purified by flash chromatography (CH2Cl2/MeOH) to provide 25C as brownish oil (300 mg, 17%).
Step 3
ethyl 5-(7-(4-phenylpiperidin-1-ylsulfonyl)-3-(trifluoromethylsulfonyloxy)-quinoxalin-2-yl)pentanoate




A mixture of 25C (300 mg, 0.6 mmol), PhNTf2 (268 mg, 0.75 mmol) and DBU (114 mg, 0.75 mmol) in DMF (6 mL) was stirred overnight. Then it was diluted with EtOAc, washed by H2O, brine, dried over Na2SO4 and concentrated under vacuum. The resulting residue was purified by flash chromatography (EtOAc/hexane) to provide 25D as colorless oil (170 mg, 45%).
Step 4
ethyl 5-(3-(4-chlorophenyl)-7-(4-phenylpiperidin-1-ylsulfonyl)quinoxalin-2-yl)pentanoate




To a solution of 25D (170 mg, 0.27 mmol) in dioxane (6 mL) in a sealed tube was added 4-chlorophenylboronic acid (84 mg, 0.54 mmol), K3PO4 (115 mg, 0.54 mmol), and KBr (64 mg, 0.54 mmol) and the mixture was degassed for 5 min. Then Pd(PPh3)4 (94 mg, 0.08 mmol) was added and the mixture was heated at 80° C. overnight. It was then taken into EtOAc and washed by H2O, brine and dried over Na2SO4, concentrated over vacuum. The resulting residue was purified by flash chromatography (EtOAc/hexane) to provide product 25E as colorless oil (40 mg, 25%).
Step 5
5-(3-(4-chlorophenyl)-7-(4-phenylpiperidin-1-ylsulfonyl)quinoxalin-2-yl)pentanoic acid




A mixture of 25E (34 mg, 0.057 mmol) and LiOH.H2O (3.6 mg, 0.086 mmol) in THF/H2O (3:1 v/v, 2.7 mL) was stirred at RT overnight. Then it was acidified by 1N HCl to PH=5 and concentrated under vacuum. The resulting residue was purified by reverse phase HPLC to provide product 25 as white solid (10 mg). MS (M+H)=564.
Example 26




Step 1
3-(5-Ethoxy-5-oxopentyl)-2-hydroxyquinoxaline-6-carboxylic acid




A suspension of 3,4-diaminobenzoic acid (0.5 g, 3.29 mmol) and diethyl 2-oxoheptane-1,7-dicarboxylate (0.757 g, 3.29 mmol, 1.0 equiv) in 10 mL AcOH/EtOH (1:1) was heated to 100° C. After 2 h of heating, the reaction mixture was cooled to RT upon which some precipitation occurred. The solid precipitate was filtered, triturated with cold EtOH (2×5 mL) and dried to afford 0.35 g of the desired compound 26A as a brown solid.
Step 2
Ethyl 5-(3-hydroxy-7-(3-phenylpyrrolidine-1-carbonyl)quinoxalin-2-yl)-pentanoate




A mixture of 26A (0.25 g, 0.785 mmol), 3-phenylpyrrolidine HCl (0.144 g, 0.785 mmol), HATU (0.597 g, 1.57 mmol, 2.0 equiv), and DIPEA (0.273 mL, 1.57 mmol, 2.0 equiv) in 5 mL CH2Cl2 was allowed to stir at RT overnight. Upon completion, water (10 mL) was added. The mixture was extracted with CH2Cl2 (2×10 mL), dried over Na2SO4, filtered and concentrated. The crude product was purified by flash chromatography (3% MeOH/CH2Cl2) to yield 0.2 g of 26B.
Step 3
Ethyl 5-(7-(3-phenylpyrrolidine-1-carbonyl)-3-(trifluoromethylsulfonyloxy)-quinoxalin-2-yl)pentanoate




To a mixture of 26B (0.1 g, 0.22 mmol) in DMF (3 mL) at 0° C. was added DBU (37 μL, 0.25 mmol, 1.1 equiv). After stirring at that temperature for 30 min was added N-phenyl-bis(trifluoromethanesulfonimide) (0.094 g, 0.26 mmol, 1.2 equiv). The reaction was stirred at 0° C. for 1 h after which water (10 mL) was added. The reaction mixture was extracted with EtOAc (2×10 mL), washed with brine, dried over Na2SO4, filtered and evaporated to dryness. Purification of the crude product by flash chromatography (40% EtOAc/hexanes) afforded 0.1 g of the desired product 26C as a pale yellow solid.
Step 4
Ethyl 5-(7-(3-phenylpyrrolidine-1-carbonyl)-3-(4-(trifluoromethyl)phenyl)-quinoxalin-2-yl)pentanoate




A mixture of 26C (100 mg, 0.17 mmol), 4-trifluoromethylbenzene boronic acid (49 mg, 0.26 mmol, 1.5 equiv), Pd(PPh3)4 (20 mg, 0.017 mmol, 10 mol %) and K3PO4 (55 mg, 0.26 mmol, 1.5 equiv) in 2.0 mL of 1,4-dioxane/H2O (4:1) was heated to 90° C. for 2 h. After cooling, the reaction mixture was loaded onto a flash column and purified by eluting with 40% EtOAc/hexanes to yield 72 mg of compound 26D.
Step 5
5-(7-(3-Phenylpyrrolidine-1-carbonyl)-3-(4-(trifluoromethyl)phenyl)-quinoxalin-2-yl)pentanoic acid




To a mixture of 26D (72 mg, 0.13 mmol) in 3 mL THF was added LiOH (22 mg, 0.25 mmol, 4.0 equiv) in 1 mL water. The reaction mixture was heated to 50° C. for 2 h after which another 11 mg of LiOH was added and stirred an additional 30 min. Upon completion, the reaction mixture was neutralized with 4 N HCl, extracted with EtOAc, dried over Na2SO4, filtered and concentrated to dryness. Purification by preparative TLC (60% CH2Cl2/30% hexanes/9% MeOH/1% AcOH) yielded 25 mg of the desired product 26 as an off white solid. MS (M+H)=548.
The following compounds were prepared in a similar manner to that described above:













No.
Compound
Name
M + H







26E




3-(3-fluorophenyl)-7-[(3- phenyl-1- pyrrolidinyl)carbonyl]-2- quinoxalinepentanoic acid
498


 


26F




3-(3-chlorophenyl)-7-[(3- phenyl-1- pyrrolidinyl)carbonyl]-2- quinoxalinepentanoic acid
514


 


26G




3-(3,5-dichlorophenyl)-7- [(3-phenyl-1- pyrrolidinyl)carbonyl]-2- quinoxalinepentanoic acid
548


 


26H




3-(2,4-difluorophenyl)-7- [(3 -phenyl-1- pyrrolidinyl)carbonyl]-2- quinoxalinepentanoic acid
516


 


26i




3-(3,5-difluorophenyl)-7- [(3-phenyl-1- pyrrolidinyl)carbonyl]-2- quinoxalinepentanoic acid
516


 


26J




3-(3-chlorophenyl)-7-[(4- phenyl-1- piperidinyl)carbonyl]-2- quinoxalinepentanoic acid
528


 


26K




7-[(4-phenyl-1- piperidinyl)carbonyl]-3-[6- (trifluoromethyl)-3- pyridinyl]-2- quinoxalinepentanoic acid
563


 


26L




3-(2-chlorophenyl)-7-[(4- phenyl-1- piperidinyl)carbonyl]-2- quinoxalinepentanoic acid
528


 


26M




7-[(4-phenyl-1- piperidinyl)carbonyl]-3-(3- pyridinyl)-2- quinoxalinepentanoic acid
495


 


26N




7-[(4-phenyl-1- piperidinyl)carbonyl]-3-[4- (trifluoromethoxy)phenyl]- 2-quinoxalinepentanoic acid
578


 


26o




3-(4-methoxyphenyl)-7- [(4-phenyl-1- piperidinyl)carbonyl]-2- quinoxalinepentanoic acid
524


 


26P




3-(4-cyanophenyl)-7-[(4- phenyl-1- piperidinyl)carbonyl]-2- quinoxalinepentanoic acid
519


 


26Q




7-[(4-phenyl-1- piperidinyl)carbonyl]-3-(5- pyrimidinyl)-2- quinoxalinepentanoic acid
496


 


26R




3-(5-chloro-3-pyridinyl)-7- [(4-phenyl-1- piperidinyl)carbonyl]-2- quinoxalinepentanoic acid
529


 


26S




7-[(4-phenyl-1- piperidinyl)carbonyl]-3-(3- thienyl)-2- quinoxalinepentanoic acid
500


 


26T




3-(1-methyl-1H-pyrazol-4- yl)-7-[(4-phenyl-1- piperidinyl)carbonyl]-2- quinoxalinepentanoic acid
498


 


26U




3-(2-acetylphenyl)-7-[(4- phenyl-1- piperidinyl)carbonyl]-2- quinoxalinepentanoic acid
536


 


26V




3-[2-methoxy-4- (trifluoromethyl)phenyl]-7- [(4-phenyl-1- piperidinyl)carbonyl]-2- quinoxalinepentanoic acid
592


 


26W




3-(2-chloro-4- methoxyphenyl)-7-[(4- phenyl-1- piperidinyl)carbonyl]-2- quinoxalinepentanoic acid
558


 


26X




3-[4- methylsulfonyl)phenyl]-7- [(4-phenyl-1- piperidinyl)carbonyl]-2- quinoxalinepentanoic acid
572


 


26Y




3-(2,4-dichlorophenyl)-7- [(4-phenyl-1- piperidinyl)carbonyl]-2- quinoxalinepentanoic acid
562


 


26Z




3-(2,4-dimethoxyphenyl)- 7-[(4-phenyl-1- piperidinyl)carbonyl]-2- quinoxalinepentanoic acid
554


 


26AA




3-[2-chloro-4- (trifluoromethyl)phenyl]-7-  [(4-phenyl-1- piperidinyl)carbonyl]-2- quinoxalinepentanoic acid
596


 


26AB




3-(4-bromophenyl)-7-[(4- phenyl-1- piperidinyl)carbonyl]-2- quinoxalinepentanoic acid
572









Example 27




In a manner similar to that previously described (e.g., Example 5), 4,5-diaminopyridine-2-carboxylic acid methyl ester (available from 4-amino-5-nitropyridine-2-carboxylic acid methyl ester by hydrogenation on Pd—C) is reacted with 2-oxo-2-phenylacetic. The resulting product 27A is advanced through the sequence previously described (chlorination with POCl3, coupling with tert-butyl pent-4-enoate, hydrolysis with LiOH, amide coupling with alpha-methylbenzylamine, and deprotection with TFA) to give 27.
Example 28




Step 1
tert-butyl 3-oxocyclopentanecarboxylate




To a solution of 3-oxocyclopentanecarboxylic acid (11.0 g, 85.9 mmol) in CH2Cl2 (200 mL) was added TFAA (23.9 mL, 171.9 mmol) and stirred at RT for 3 h. Then t-BuOH (64.4 mL, 687.5 mmol) was added and the reaction was stirred overnight. The mixture was then diluted with CH2Cl2 and washed with H2O and brine, dried (Na2SO4), concentrated and purified by flash chromatography to give product 28A as colorless liquid (10.8 g).
Step 2
tert-butyl 3-methylenecyclopentanecarboxylate




A mixture of Ph3PMeBr (35.7 g, 100 mmol) and KOtBu (11.2 g, 100 mmol) in THF (200 mL) was stirred at RT for 3 h, and then was treated with 28A (9.2 g, 50 mmol). The resulting mixture was stirred at RT overnight, then diluted with EtOAc and washed with H2O and brine. The organic layer was dried (Na2SO4), concentrated and purified by flash chromatography to give product 28B as colorless liquid (9.5 g).
Step 3
methyl 3-((3-(tert-butoxycarbonyl)cyclopentyl)methyl)-2-(4-chlorophenyl)-quinoxaline-6-carboxylate




To a solution of 28B (2.1 g, 11.3 mmol) in THF (12 mL) was added 9-BBN (28.2 mL, 0.4 M/hexane) and stirred for 3 h at RT. Compound 16B (1.5 g, 4.5 mmol), Pd2(dba)3 (103 mg, 0.11 mmol), cataCXium A (161 mg, 0.45 mmol), K2CO3 (1.24 g, 9.0 mmol) and H2O (3 mL) were then added into the above mixture and heated at 80° C. overnight. The reaction was diluted with EtOAc, washed with H2O, brine, dried (Na2SO4), concentrated and purified by flash chromatography to give product 28C as colorless oil (2.0 g, cis/trans=3:1).
Step 4
3-((3-(tert-butoxycarbonyl)cyclopentyl)methyl)-2-(4-chlorophenyl)-quinoxaline-6-carboxylic acid




A mixture of 28C (440 mg, 0.92 mmol) and LiOH-2O (46 mg, 1.10 mmol) was stirred at RT overnight. Then the reaction was acidified with 1N HCl to pH=5 and concentrated under reduced pressure to give crude residue, which was purified by HPLC to give product 28D as colorless oil (429 mg, cis/trans=3:1).
Step 5
tert-butyl 3-((3-(4-chlorophenyl)-7-((R)-1-(4-fluorophenyl)ethylcarbamoyl)-quinoxalin-2-yl)methyl)cyclopentanecarboxylate




To a solution of 28D (429 mg, 0.92 mmol) in DMF (10 mL) was added (R)-1-(4-fluorophenyl)ethanamine (258 mg, 1.84 mmol), HATU (699 mg, 1.84 mmol) and DIPEA (0.32 mL, 1.84 mmol) and the mixture was stirred at RT overnight. Then the reaction was diluted with EtOAc and washed with H2O, brine, dried (Na2SO4), concentrated and purified by flash chromatography to give product 28E as colorless oil (320 mg, cis/trans=3:1).
Step 6
3-((3-(4-chlorophenyl)-7-((R)-1-(4-fluorophenyl)ethylcarbamoyl)quinoxalin-2-yl)methyl)cyclopentanecarboxylic acid




To a solution of 28E (320 mg, 0.54 mmol) in CH2Cl2 (10 mL) was added TFA (0.84 mL) and the mixture was stirred overnight. The reaction was concentrated under reduced pressure and the residue was purified by HPLC to give product 28 as off-white powder (170 mg, cis/trans=3:1). LCMS (M+H)=532.
Example 29




Step 1
methyl 2-hydroxy-3-methylquinoxaline-6-carboxylate




A suspension of methyl 3,4-diaminobenzoate (5.0 g, 30.09 mmol) and ethyl pyruvate (3.34 mL, 30.09 mmol, 1.0 equiv) in 60 mL AcOH/EtOH (1:1) was heated to 100° C. After 2 h of heating, the reaction mixture was cooled to RT upon which some precipitation occurred. The solid precipitate was filtered, triturated with cold EtOH (2×10 mL) followed by Et2O (2×30 mL) and dried to afford 2.42 g of compound 29B as a brown solid. To the mother liquor was added 150 mL Et2O upon which more precipitation occurred. The solid precipitate was filtered, triturated with Et2O and dried to afford 1.16 g of the desired compound 29A as a brown solid.
Step 2
methyl 3-methyl-2-(trifluoromethylsulfonyloxy)quinoxaline-6-carboxylate




To a mixture of 29A (1.81 g, 8.29 mmol) in DMF (30 mL) at 0° C. was added DBU (1.37 mL, 9.12 mmol, 1.1 equiv). After stirring at that temperature for 30 min, N-phenyl-bis(trifluoromethanesulfonimide) (3.56 g, 9.95 mmol, 1.2 equiv) was added. The reaction was stirred at 0° C. for 1 h after which water (50 mL) was added. The reaction mixture was extracted with EtOAc (2×30 mL), washed with brine, dried over Na2SO4, filtered and evaporated to dryness to yield 2.9 g of 29C as a brown solid. The product was used as such without further purification.
Step 3
methyl 2-(4-chlorophenyl)-3-methylquinoxaline-6-carboxylate




A mixture of 29C (2.9 g, 8.3 mmol), 4-chlorobenzeneboronic acid (1.95 g, 12.45 mmol, 1.5 equiv), Pd(PPh3)4 (958 mg, 0.83 mmol, 10 mol %) and K3PO4 (2.64 g, 12.45 mmol, 1.5 equiv) in 30 mL of 1,4-dioxane/H2O (4:1) was heated to 90° C. for 2 h. After cooling, the reaction mixture was concentrated to half its volume, loaded onto a flash column and purified by eluting with 40% EtOAc/hexanes to yield 1.49 g of compound 29D.
Step 4
methyl 3-(bromomethyl)-2-(4-chlorophenyl)quinoxaline-6-carboxylate




To a mixture of 29D (1.39 g, 4.44 mmol) in 25 mL AcOH was added 1 mL HBr (33% in AcOH) followed by pyridinium tribromide (1.56 g, 4.88 mmol, 1.1 equiv). After all the starting material had been consumed, H2O (30 mL) was added. The reaction mixture was extracted with Et2O (2×20 mL), dried over Na2SO4, filtered and evaporated to dryness to yield a mixture of products. Purification by flash chromatography (5% EtOAc/hexanes) afforded 250 mg of the desired product 29E as a white powder and 120 mg of 29F.
Step 5
methyl 3-((3-tert-butoxy-3-oxopropoxy)methyl)-2-(4-chlorophenyl)-quinoxaline-6-carboxylate




To a mixture of NaH (54 mg, 1.35 mmol, 3.0 equiv) in 5 mL THF at 0° C. was added tert-butyl 3-hydroxypropionate (200 μL, 1.35 mmol, 3.0 equiv) dropwise. After 30 min, 29E (175 mg, 0.45 mmol, 1.0 equiv) in THF (3 mL) was added dropwise and the reaction was gradually warmed to RT. After 3 h of stirring, 5 mL of a 0.5 N HCl solution was added. The reaction mixture was extracted with CH2Cl2 (2×5 mL), dried over Na2SO4, filtered and evaporated to dryness. Purification by preparative TLC (20% EtOAc/hexanes) afforded 30 mg of the desired product 29F.
Steps 6-8
3-((3-(4-chlorophenyl)-7-(4-phenylpiperidine-1-carbonyl)quinoxalin-2-yl)methoxy)propanoic acid




In a manner similar to that previously described, compound 29F was sequentially treated with LiOH (2 eq., THF—H2O, 50° C., 2 h), coupled with 4-phenylpiperidine (HATU, DIPEA, DCM, RT overnight), and then deprotected with TFA to afford the title compound 29. LCMS (M+H)=530.
Example 30




Step 1
methyl 2-(4-chlorophenyl)-3-((3-cyanocyclobutyl)methyl)quinoxaline-6-carboxylate




A stirred solution of 3-methylenecyclobutanecarbonitrile (0.218 g, 2.34 mmol, 1.5 equiv) in THF (1 mL) under nitrogen was cooled to 0° C. 9-BBN (0.5 N in THF, 5.62 mL, 2.81 mmol, 1.8 equiv) was added and the solution was heated to 60° C. for 3 h. After this time, the reaction mixture was cooled to RT and methyl 3-chloro-2-(4-chlorophenyl)quinoxaline-6-carboxylate 16B (520 mg, 1.56 mmol), Pd(dppf)2Cl2.CH2Cl2 (255 mg, 0.312 mmol, 20 mol %) and K3PO4 (993 mg, 4.68 mmol, 3.0 equiv) were added. The reaction mixture was degassed and was heated at 60° C. under nitrogen for 12 h. After this time, the reaction was cooled to room temperature and diluted with water and CH2Cl2. The aqueous layer was separated and extracted with CH2Cl2 (2×15 mL), dried over Na2SO4, filtered and concentrated. The residue was purified by flash chromatography (10-15% EtOAc/hexanes) to yield 256 mg of the desired product 30A.
Steps 2-3
3-((3-(4-chlorophenyl)-7-(4-phenylpiperidine-1-carbonyl)quinoxalin-2-yl)methyl)cyclobutanecarbonitrile




In a manner similar to that previously described, 30A was hydrolyzed with LiOH (2 eq, THF—H2O, 3 h, RT) and then coupled with 4-phenylpiperidine (1 eq., 2 eq. HATU, 2 eq. DIPEA, DCM, overnight, RT) to provide 30C.
Steps 4-5
3-(((3-(4-chlorophenyl)-7-(4-phenylpiperidine-1-carbonyl)quinoxalin-2-yl)methyl)cyclobutanecarboxylic acid




To a solution of 30C (150 mg, 0.29 mmol) in 5 mL MeOH was passed HCl (g) at 0° C. After 3 min of passing, the reaction mixture was allowed to stir at room temperature for 1 h. The excess solvent and HCl was concentrated, diluted with H2O and extracted with CH2Cl2 (2×10 mL), dried over Na2SO4, filtered and concentrated. The crude product was purified by flash chromatography (20-30% EtOAc/hexanes) to yield 50 mg of the desired product 30D.
Step 6
3-((3-(4-chlorophenyl)-7-(4-phenylpiperidine-1-carbonyl)quinoxalin-2-yl)methyl)cyclobutanecarboxylic acid




To a mixture of 30D (50 mg, 0.09 mmol) in 2 mL THF was added LiOH.H2O (15 mg, 0.36 mmol, 4.0 equiv) in 1 mL water. The reaction mixture was heated to 50° C. for 2 h. Upon completion, the reaction mixture was neutralized with 2 N HCl, extracted with CH2Cl2 (2×5 mL), dried over Na2SO4, filtered and concentrated to dryness. The crude product was purified by preparative TLC (60% CH2Cl2/30% hexanes/9% MeOH/1% AcOH) to yield 44 mg of the desired product 30. LCMS (M+H)=540.
Compound 30E was prepared analogous manner using (R)-1-(4-fluorophenyl)ethanamine in Step 3. LCMS (M+H)=518.




Example 31




Step 1
2-(azetidin-3-yl)-1H-benzo[d]imidazole




To a solution of compound 1,2-phenylenediamine (1Mg, 9.25 mmol) and 1-Boc-3-azetidinecarboxaldehyde (2.1 g, 11.10 mmol) in 60 mL of IPA was added Pd/C (10%, 0.8 g). After heating at 80° C. for 2 h, the mixture was cooled down to RT, filtered through the CELITE, concentrated and purified (2.1 g). The product was treated with 4N HCl (8 mL) in 1,4-dioxane (20 mL) to give 2 g of compound 31A (Yield=83%).
Step 2
5-(7-(3-(1H-benzo[d]imidazol-2-yl)azetidine-1-carbonyl)-3-(4-fluorophenyl)quinoxalin-2-yl)pentanoic acid




To a solution of compound 31A (0.11 g, 0.46 mmol) and 5D (0.13 g, 0.31 mmol) in 5 mL of DCM was added DEC (0.088 g, 0.46=1101), HOBT (0.062 g, 0.046 mmol), and DIEA (0.16 mL, 0.93 mmol). After stirring at RT for 20 h, the mixture was extracted with DCM and saturated NaHCO3 (aq), dried over Na2SO4, concentrated and purified (0.063 g). The product 31B was deprotected with 0.5 mL of TFA in 5 mL of DCM to give the title compound 31 (0.051 g). MS (M+H)=524.
Compound 31C was prepared analogous manner using 2-aminophenol in Step 1. MS (M+H)=507.




Example 32




Step 1
tert-Butyl 2-(3-allylphenyl)acetate




To a solution of 19A (2.0 g, 7.38 mmol) in toluene (20 mL) was added allyltri-n-butyltin (2.382 mL, 9.59 mmol), and Pd(Ph3P)4 (0.852 g, 0.738 mmol). The reaction mixture was degassed and heated at 90° C. overnight under a nitrogen atmosphere. The mixture was diluted with EtOAc and washed with H2O. Potassium fluoride (10 g, 172 mmol) and 200 mL of H2O was added to the organic mixture and the mixture was stirred for 1.5 h. The layers were split and filtered the solids through CELITE, dried over MgSO4, filtered and concentrated. The concentrate was purified by flash chromatography (over 120 g of silica gel) in 0-10% Et2O in hexanes to yield 1.24 g (73% yield) of 19B.
Step 2-5
2-(3-(3-(3-(4-chlorophenyl)-7-(4-phenylpiperidine-1-carbonyl)quinoxalin-2-yl)propyl)phenyl)acetic acid




In a manner similar to that previously described, 19B was coupled with methyl 3-chloro-2-(4-chlorophenyl)quinoxaline-6-carboxylate (16B) and hydrolyzed with LiOH. The resulting acid 32D was coupled with 4-phenylpiperidine and deprotected with TFA to provide 32. MS (M+H)=604.
Example 33




Step 1-2
(R)-2-(4-chlorophenyl)-3-methyl-N-(1,2,3,4-tetrahydronaphthalen-1-yl)quinoxaline-6-carboxamide
In a manner similar to that previously described, 33A was hydrolyzed with LiOH and coupled with (R)-tetrahydronaphthalen-1-amine to provide 33C.
Step 3
(R)-2-(4-chlorophenyl)-3-formyl-N-(1,2,3,4-tetrahydronaphthalen-1-yl)quinoxaline-6-carboxamide
To 33C (50 mg, 0.117 mmol) in dioxane (1 mL) was added selenium dioxide (19.45 mg, 0.175 mmol) in a sealed tube. The mixture was heated at 100° C. for 12 h. After cooling, the excess solvent was concentrated and the reaction mixture was purified by preparative TLC eluting with 3:1 Hex/EtOAc, then 2:1 Hex/EtOAc to yield 49 mg of 33D.
Step 4
methyl 5-(3-(4-chlorophenyl)-7-(((R)-1,2,3,4-tetrahydronaphthalen-1-yl)carbamoyl)quinoxalin-2-yl)-5-hydroxypentanoate
To 33D (246 mg, 0.557 mmol) and CuCN (4.99 mg, 0.056 mmol) in DCM (5 mL) was added 4-ethoxy-4-oxobutylzinc bromide in THF (3.34 mL, 1.670 mmol). The reaction was stirred at 22° C. overnight, after which it was quenched with water and 1 N hydrochloric acid (1 mL) and extracted with ethyl acetate and with dichloromethane. The combined organics was dried over MgSO4, filtered and concentrated. Purification by flash chromatography (over 80 g of silica gel) in 0-25-50% EtOAc in hexanes afforded 59 mg (yield=19%) of 33E.
Step 5
5-(3-(4-chlorophenyl)-7-(((R)-1,2,3,4-tetrahydronaphthalen-1-yl) carbamoyl)quinoxalin-2-yl)-5-hydroxypentanoic acid
To 33E (10.1 mg, 0.018 mmol) in THF (0.1 mL), Water (0.100 mL), and MeOH (0.100 mL) was added LiOH (3.04 mg, 0.072 mmol). The reaction was stirred at 22° C. for 3 h. The mixture was quenched with water, acidified with 1 N HCl to pH<1 and extracted with dichloromethane. The combined organics was dried over MgSO4, filtered and concentrated. The mixture was purified by preparative TLC in 10:1 DCM/MeOH to obtain 3.0 mg (yield=29%) of desired product 2. MS (M+H)=530.
Example 34




Step 1
(R)-methyl 5-(3-(4-chlorophenyl)-7-((1,2,3,4-tetrahydronaphthalen-1-yl)carbamoyl)quinoxalin-2-yl)-5-oxopentanoate
To 33E (50 mg, 0.090 mmol) in DCM (1 mL) was added Dess-Martin periodinane (76 mg, 0.179 mmol). The reaction mixture was stirred at 22° C. overnight. After MS indicated the reaction was complete, the mixture was loaded on a 1 mm preparative TLC plate and eluted with 2:1 Hex/EtOAc twice to yield 13 mg (26% yield) of 34A.
Step 2
(R)-5-(3-(4-chlorophenyl)-7-((1,2,3,4-tetrahydronaphthalen-1-yl)carbamoyl)quinoxalin-2-yl)-5-oxopentanoic acid
To 34A (13.0 mg, 0.023 mmol) in THF (0.3 mL), MeOH (0.300 mL), and water (0.300 mL) was added LiOH (3.92 mg, 0.094 mmol). The reaction was stirred at 22° C. and purified by preparative TLC in 10:1 DCM/MeOH to obtain 5.0 mg (41% yield) of product 34. MS (M+H)=528.
Example 35




Step 1
methyl 5-(3-(4-chlorophenyl)-7-(((R)-1,2,3,4-tetrahydronaphthalen-1-yl)carbamoyl)quinoxalin-2-yl)-5-fluoropentanoate
To 33E (40 mg, 0.072 mmol) in dichloroethane (1 mL) was added DAST (0.024 mL, 0.179 mmol). The reaction mixture was stirred at 22° C. overnight. The reaction was quenched with aqueous ammonium chloride and extracted with dichloromethane. The combined organics was dried over MgSO4, filtered and concentrated. The mixture was purified on a 1 mm preparative TLC plate in 2:1 Hex/EtOAc to yield 38 mg of 35A.
Step 2
5-(3-(4-chlorophenyl)-7-(((R)-1,2,3,4-tetrahydronaphthalen-1-yl)carbamoyl)quinoxalin-2-yl)-5-fluoropentanoic acid
To 35A (38.7 mg, 0.069 mmol) in THF (0.3 mL), MeOH (0.300 mL) and water (0.300 mL) was added LiOH (11.60 mg, 0.276 mmol). The mixture was stirred at 22° C. for 2 h. The reaction was quenched with water and hydrochloric acid and extracted with dichloromethane. The combined organics were dried over MgSO4, filtered and concentrated. The mixture was purified by preparative TLC in 10:1 DCM/MeOH to yield 2.5 mg of 35. MS (M+H)=532.
Example 36




Steps 1-4
(R)-4-(7-((1-(4-fluorophenyl)ethyl)carbamoyl)-3-phenylquinoxalin-2-yl)butanoic acid
In a manner similar to that previously described, 36A was coupled with methyl 3-chloro-2-phenylquinoxaline-6-carboxylate (6B) and hydrolyzed with LiOH. The resulting acid 36C was coupled with (R)-1-(4-fluorophenyl)ethanamine to provide 36D which was deprotected with TFA to provide 36.
Example 37




Step 1
2,2-difluoro-5-(7-(methoxycarbonyl)-3-phenylquinoxalin-2-yl)pentanoic acid
9-Borabicyclo[3.3.1]nonane (32.6 mL, 16.32 mmol) was added to a stirred, cooled 0° C. mixture of 2,2-difluoropent-4-enoic acid (1.110 g, 8.16 mmol) and the mixture was stirred at 0° C. for 30 min. before it was warmed to room temperature and stirred for overnight. Reactant 6B (1.4 g, 4.08 mmol), butyl di-1-adamantylphosphine (0.117 g, 0.326 mmol), potassium phosphate tribasic (1.732 g, 8.16 mmol) and tris(dibenzylideneacetone)dipalladium(0) chloroform adduct (0.084 g, 0.082 mmol) were dissolved in H2O (13.60 mL) and THF (27.2 mL). The solution was added to the 9-BBN adduct and stirred at 80° C. under nitrogen for 1 h. The mixture was cooled, diluted with ethyl acetate (30 mL), washed with hydrochloric acid (1 M, 1×20 mL), dried (Na2SO4), filtered and the solvent was evaporated under reduced pressure. The residue was purified by column chromatography on silica gel (50 g silica gel), eluting with EtOAc/Hexanes (10% to 80%) to give 37A as a yellow foam. MS (M+H)=508.
Step 2
(R)-2,2-difluoro-5-(7-((1-(4-fluorophenyl)ethyl)carbamoyl)-3-phenylquinoxalin-2-yl)pentanoic acid
Isopropylmagnesium chloride (281 μl, 0.562 mmol) was added to a stirred, cooled 0° C. mixture of (R)-1-(4-fluorophenyl)ethylamine (31.3 mg, 0.225 mmol) and 6A (45 mg, 0.112 mmol) in THF (1124 μl) and the mixture was stirred at room temperature for overnight. The reaction was quenched by NH4Cl and worked up. The residue was purified by preparative HPLC Reverse phase (C-18), eluting with Acetonitrile/Water+0.1% TFA, to give 20 mg (34% yield) of 37 as an orange solid.
Example 38




In a manner similar to that described previously (Step 3), ethyl 2-fluoropent-4-enoate (prepared from allyl iodide of ethyl fluoroacetate, NaH, and allyl iodide in HMPA and benzene, 80° C., 12 h as described in J. Am. Chem. Soc. 2003, 125, 15521, S-21) was sequentially reacted with 9-BBN and 6B to provide the desired ethyl ester, which was subsequently treated with LiOH to afford 2-fluoro-5-(3-phenyl-7-(((R)-1,2,3,4-tetrahydronaphthalen-1-yl)carbamoyl)quinoxalin-2-yl)pentanoic acid (38, LCMS, M+H=498).
The enantiomers of 38 were separated by supercritical fluid chromatography (AD column, injected in MeOH, 55% MeOH co-solvent and 45% CO2) to provide 38A (LCMS, M+H=498) and 38B. MS (M+H)=498.
Example 39




Step 1
(R)-tert-butyl 4-(4-fluorophenyl)-2-methylpiperazine-1-carboxylate
A mixture of (R)-tert-butyl 2-methylpiperazine-1-carboxylate (1.7 g, 8.49 mmol), 1-bromo-4-fluorobenzene (1.63 g, 9.34 mmol, 1.1 eq), Pd2(dba)3 (0.78 g, 0.85 mmol, 0.1 eq), P(tBu)3 (0.343 g, 1.70 mmol, 0.2 eq) and t-BuOK (0.952 g, 8.49 mmol, 1.0 eq.) in toluene (8.0 mL) under nitrogen atmosphere was heated to 110° C. for 1 h in a microwave. After the reaction was complete, the mixture was diluted with water (100 mL) and extracted with CH2Cl2, dried over Na2SO4, filtered and evaporated to dryness. The crude reaction mixture was purified by column chromatography eluting with 10-20% EtOAc/hexanes to yield 1.9 g of 39A.
Step 2
(R)-1-(4-fluorophenyl)-3-methylpiperazine
To a solution of 39A (1.9 g, 6.45 mmol) in CH2Cl2 (10 mL) was added TFA (3.0 mL, 38.7 mmol, 6.0 eq) and the reaction was stirred at room temperature for 2 h. After reaction was complete, the excess TFA was concentrated to yield product 39B which was used as such without any further purification.
Step 3
(R)-tert-butyl 5-(7-(4-(4-fluorophenyl)-2-methylpiperazine-1-carbonyl)-3-phenylquinoxalin-2-yl)pentanoate
To a flask containing the carboxylic acid 6D (2.2 g, 5.41 mmol) in CH2Cl2 (40 mL) was added (R)-1-(4-fluorophenyl)-3-methylpiperazine (1.16 g, 5.95 mmol, 1.1 eq), Hunig's base (3.75 mL, 21.65 mmol, 4.0 eq) and HATU (4.12 g, 10.82 mmol, 2.0 eq). The mixture was stirred at ambient temperature overnight after which the mixture was concentrated and purified by flash chromatography (10-30% EtOAc/hexanes) to yield 2.3 g of 39C (69% yield).
Step 4
(R)-5-(7-(4-(4-fluorophenyl)-2-methylpiperazine-1-carbonyl)-3-phenylquinoxalin-2-yl)pentanoic acid
To a solution of 39C (2.3 g, 3.95 mmol) in CH2Cl2 (10 mL) was added TFA (1.83 mL, 23.68 mmol, 6.0 eq), and the reaction was stirred at room temperature for 2 h. After the reaction was complete, the excess TEA was concentrated to yield product 39 which was purified by column chromatography eluting with 2-4% MeOH/CH2Cl2 to yield 1.9 g of desired product 39. 1H NMR (DMSO, 500 MHz) δ 8.16 (d, 1H, J=8.5 Hz), 8.08 (s, 1H), 7.82 (dd, 1H, J=1.5 Hz, J=8.5 Hz), 7.71-7.69 (m, 2H), 7.60-7.57 (m, 3H), 7.08 (t, 2H, J=9 Hz), 7.00-6.97 (m, 2H), 3.61-3.36 (m, 3H), 3.03 (1, 2 H, J=7.5 Hz), 2.91 (d, 1H, J=9.5 Hz), 2.72 (dt, 1H, J=2.5 Hz, J=11.5 Hz, J=14.5 Hz), 2.47 (m, 2H), 2.16 (t, 2H, J=7 Hz), 1.74-1.70 (m, 2H), 1.51-1.46 (m, 2H), 1.42-1.32 (m, 3H). MS (M+H)=527.
Example 40
(R)-5-(7-((6-fluoro-1,2,3,4-tetrahydronaphthalen-1-yl)carbamoyl)-3-phenylquinoxalin-2-yl)pentanoic acid




In a manner similar to that previously described, 6C was hydrolyzed with LiOH. The resulting acid 6D was coupled with (R)-6-fluoro-1,2,3,4-tetrahydronaphthalen-1-amine and deprotected with TFA to provide 40. 1H NMR (DMSO, 500 MHz) δ 11.97 (s, 1H), 9.17 (d, 1H, J=8.5 Hz), 8.65 (d, 1H, J=2 Hz), 8.28 (dd, 1H, J=1.5 Hz, J=8.5 Hz), 8.13 (d, 1H, J=9 Hz), 7.71-7.69 (m, 2H), 7.58-7.56 (m, 2H), 7.30 (t, 1H, J=7 Hz), 7.02-6.98 (m, 2H), 5.28 (q, 1H, J=7 Hz, J=12 Hz), 3.02 (t, 2H, J=7.5 Hz), 2.86-2.75 (m, 2H), 2.15 (t, 2H, J=7 Hz), 2.03-1.97 (m, 2H), 1.94-1.87 (m, 1H), 1.80-1.71 (m, 3H), 1.51-1.45 (m, 2H). MS (M+H)=498.
Example 41
(R)-5-(7-(2-methyl-4-phenylpiperazine-1-carbonyl)-3-phenylquinoxalin-2-yl)pentanoic acid




In a manner similar to that previously described, 6C was hydrolyzed with LiOH. The resulting acid 6D was coupled with (R)-3-methyl-1-phenylpiperazine and deprotected with TFA to provide 41. 1H NMR (DMSO, 500 MHz) δ 8.16 (d, 1H, J=8.5 Hz), 8.08 (d, 1H, J=1.5 Hz), 7.82 (dd, 1H, J=2 Hz, J=8.5 Hz), 7.71-7.69 (m, 2H), 7.59-7.57 (m, 3H), 7.26-7.22 (m, 2H), 6.96 (d, 2H, J=8 Hz), 6.82 (t, 1H, J=7 Hz), 3.71-3.49 (m, 3H), 3.03 (t, 2H, J=7.5 Hz), 2.98-2.92 (m, 1H), 2.76 (dt, 1H, J=3.5 Hz, J=12 Hz, J=15.5 Hz), 2.44 (m, 2H), 2.16 (t, 2H, J=7.5 Hz), 1.76-1.70 (m, 2H), 1.51-1.45 (m, 2H), 1.43-1.30 (m, 3H). MS (M+H)=509.
Example 42
2-(3-(2-(3-(4-chlorophenyl)-7-(4-phenylpiperidine-1-carbonyl)quinoxalin-2-yl)ethyl)-5-fluorophenyl)acetic acid




In a manner similar to that previously described for compound 19, 3-bromo-5-fluorophenylacetic acid was reacted with TFAA and t-BuOH to provide tert-butyl 2-(3-bromo-5-fluorophenyl)acetate 42A and coupled with potassium vinyltrifluoroborate to afford 42B. The resulting vinyl compound 42B was coupled with methyl 3-chloro-2-(4-chlorophenyl)quinoxaline-6-carboxylate (16B) and hydrolyzed with LiOH. The resulting acid 42D was coupled with 4-phenylpiperidine and deprotected with TFA to provide 42 (LCMS, M+H=608).
Example 43
Parallel Preparation of Compounds Bearing a 4-Chlorophenyl R2 Substituent from Compound 16C




Into twenty-four 2 dram vials were added the amines (R′)(R)NH (0.044 mmol), followed by a DCM (1.0 mL) solution of 3-(5-tert-butoxy-5-oxopentyl)-2-(4-chlorophenyl)quinoxaline-6-carboxylic acid 16C (15.0 mg, 0.034 mmol) and DIBA (24 μL, 0.137 mmol). The mixtures shaken at room temperature for 5 min., then 1-propanephosphonic acid cyclic anhydride (50% w/w in EtOAc) (30.0 μL, 0.050 mmol) was added, and then the vials were capped and shaken at room temperature overnight.
Trifluoroacetic acid (50%) in DCM (1.0 mL, 6.49 mmol) was then added to each of the vials. The vials were then recapped and shaken at room temperature for 2 hrs. The solvent was then concentrated in vacuo from each vial.
DMSO (1.0 mL) was then added to each of the vials. The vials were sonicated until all were in solution, and the DMSO solutions passed through a filter plate under vacuum into a deep-well plate and purified by reverse-phase chromatography.
Example 44
Step 1




To the mixture of compound 6B (8.6 g, 28.8 mmol) in N-methyl-2-pyrrolidinone (50 mL), H-gamma-ABU-OtBu HCl salt (7.04 g, 36.0 mmol), and Cs2CO3 (23.45 g, 72.0 mmol) was stirred and heated to 100° C. for 16 hours. The reaction mixture was worked up with ethyl acetate (200 mL) and brine (150 mL×3) to give a brown paste. The paste was separated by flash chromatography (220 g silica gel) eluting with 10 ̃20% EtOAc/Hexanes to give compound 44A (10.5 g, 82%).
Step 2




The mixture of 44A (10.3 g, 24.4 mmol) in THF/MeOH/H2O (50 mL, 2:2:1) and LiOH—H2O (2.05 g, 48.9 mmol) was stirred at RT for 2 hours. The reaction mixture was neutralized with 1 N HCl, extracted with CH2Cl2 (100 mL×2). The organic solution was dried (Na2SO4) and concentrated to give compound 44B (9.4 g, 76%).
Step 3




A mixture of 39A (0.95 g, 3.23 mmol) in dichloromethane (2 mL) and 4 N HCl in 1,4-dioxane (4.03 mL. 16.14 mmol) was stirred at room temperature for 2 hours. The excess HCl and solvents were removed in vacuo to give 0.74 g compound 39B.HCl (0.74 g, 99%).
Step 4




The mixture of compound 44B (2.0 g, 4.91 mmol) in dichloromethane (20 mL), compound 39B.HCl (1.13 g, 4.91 mmol), Hunig's base (1.59 g, 12.27 mmol) and HATU (2.80 g, 7.36 mmol) was stirred at room temperature for 16 h. The reaction mixture was worked up with dichloromethane (100 mL)/water (40 mL), and purified by flash chromatography (120 g silica gel) eluting with 20 ̃30% EtOAc/Hexanes to give compound 44C (1.6 g, 51%).
Step 5
(R)-4-((7-(4-(4-fluorophenyl)-2-methylpiperazine-1-carbonyl)-3-phenylquinoxalin-2-yl)amino)butanoic acid




A mixture of compound 44C (1.4 g, 2.40 mmol) in dichloromethane (2 mL) and TFA (2.73 g, 23.99 mmol) was stirred at room temperature for 2 h. The excess TFA and the solvent were removed to give a brown gum. The gum was neutralized with 2 N NaOH and purified by flash column chromatography (40 g silica gel) eluting with 40 ̃60% EtOAc/hexanes to give compound 44 (1.15 g, 86%). MS (M+H)=528.
Compound 44H, shown below, was prepared in a similar manner. 1H NMR (DMSO, 500 MHz) δ 7.84 (d, 1H, J=8.5 Hz), 7.74-7.72 (m, 2H), 7.61 (d, 1H, J=1.5 Hz), 7.60-7.57 (m, 2H), 7.36 (dd, 1H, J=1.5 Hz, J=8 Hz), 7.10-7.05 (m, 2H), 7.02-6.99 (m, 2H), 6.95-6.88 (m, 1H), 3.88-3.74 (m, 2H), 3.63-3.5 (m, 2H), 3.46 (q, 2H, J=6.5 Hz, J=12.5 Hz), 3.38-3.05 (m, 4H), 2.29 (t, 2H, J=7 Hz), 1.86 (t, 2H, J=6.5 Hz).
The following compounds were prepared in a similar manner to that described above in Example 44:













No.
Structure
Name
M + H







44D




(R)-4-((7-((1-(4- fluorophenyl)ethyl) carbamoyl)-3- phenylquinoxalin-2- yl)amino)butanoic acid
473


 


44E




(R)-4-((7-((6-fluoro-1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)-3- phenylquinoxalin-2- yl)amino)butanoic acid
499


 


44F




4-((3-phenyl-7-(4- phenylpiperazine-1- carbonyl)quinoxalin-2- yl)amino)butanoic acid
496


 


44G




(R)-4-(((7-((1-(4- cyanophenyl)ethyl) carbamoyl)-3- phenylquinoxalin-2- yl)amino)butanoic acid
480


 


44H




4-((7-(4-(4- fluorophenyl)piperazine-1- carbonyl)-3- phenylquinoxalin-2- yl)amino)butanoic acid
514


 


44i




(R)-4-((3-phenyl-7- ((1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)quinoxalin-2- yl)amino)butanoic acid
481


 


44J




4-((7-(4-(4- fluorophenyl)piperidine-1- carbonyl)-3- phenylquinoxalin-2- yl)amino)butanoic acid
513


 


44K




4-((7-(4-(4- chlorophenyl)piperidine-1- carbonyl)-3- phenylquinoxalin-2- yl)amino)butanoic acid
513


 


44L




4-((7-(4-(4- chlorophenyl)piperazine-1- carbonyl)-3- phenylquinoxalin-2- yl)amino)butanoic acid
529


 


44M




(R)-4-((3-phenyl-7-(3- phenylpyrrolidine-1- carbonyl)quinoxalin-2- yl)amino)butanoic acid
481


 


44N




(R)-4-((7-(2-methyl-4- phenylpiperazine-1- carbonyl)-3- phenylquinoxalin-2- yl)amino)butanoic acid
510









The following compounds were prepared following procedures similar to those exemplified in the examples above.
















M +


No.
Structure
Name
H







100




7-[[[(4- chlorophenyl)methyl] carbonyl]-3-(4-fluorophenyl)- 2-quinoxalinepentanoic acid
492


 


101




7-[[[(3- chlorophenyl)methyl]amino] carbonyl]-3-(4-fluorophenyl)- 2-quinoxalinepentanoic acid
492


 


102




3-(4-fluorophenyl)-7-[[(2,2,2- trifluoro-1(S)- phenylethyl)amino]carbonyl]- 2-quinoxalinepentanoic acid
526


 


103




7-[[(2,3-dihydro-1H-inden-2- yl)amino]carbonyl]-3-(4- fluorophenyl)-2- quinoxalinepentanoic acid
484


 


104




7-[[[(4- cyanophenyl)methyl]amino] carbonyl]-3-(4-fluorophenyl)- 2-quinoxalinepentanoic acid
483


 


105




3-(4-fluorophenyl)-7-[(4- phenyl-1- piperidinyl)carbonyl]-2- quinoxalinepentanoic acid
512


 


106




3-(4-fluorophenyl)-7-[(3- phenyl-1- pyrrolidinyl)carbonyl]-2- quinoxalinepentanoic acid



 


107




3-(4-fluorophenyl)-7-[[[[4- (trifluoromethoxy)phenyl] methyl]amino]carbonyl]-2- quinoxalinepentanoic acid
542


 


108




7-[[[(3,5- dichlorophenyl)methyl]amino] carbonyl]-3-(4- fluorophenyl)-2- quinoxalinepentanoic acid
526


 


109




3-(4-fluorophenyl)-7-[[[(4- fluorophenyl)methyl]amino] carbonyl]-2- quinoxalinepentanoic acid
476


 


110




3-(4-fluorophenyl)-7-[[[1(R)- (4-fluorophenyl)ethyl] amino]carbonyl]-2- quinoxalinepentanoic acid
490


 


111




3-(4-fluorophenyl)-7-[[[1(S)- (4-fluorophenyl)ethyl] amino]carbonyl]-2- quinoxalinepentanoic acid
490


 


112




3-(4-fluorophenyl)-7-[[[[4- (trifluoromethyl)phenyl]methyl] yl]amino]carbonyl]-2- quinoxalinepentanoic acid
526


 


113




7-[[4-[2,3-dihydro-3-(2- methoxyethyl)-2-oxo-1H- benzimidazol-1-yl]-1 - piperidinyl]carbonyl]-3- phenyl-2- quinoxalinepentanoic acid
608


 


114




7-[[4-[(E)-(methoxyimino)-2- pyridinylmethyl]-1- piperidinyl]carbonyl]-3- phenyl-2- quinoxalinepentanoic acid
552


 


115




3-phenyl-7-[[4-[2-(2- pyridinyl)-1H-benzimidazol- 1-yl]-1-piperidinyl]carbonyl]- 2-quinoxalinepentanoic acid
612


 


116




7-(4-phenyl-1,2,3,6- tetrahydropyridine-1- carbonyl)-3-phenyl-2- quinoxalinepentanoic acid
492


 


117




7- [[4-(2,3-dihydro-2-oxo-1H- benzimidazol-1-yl)-1- piperidinyl]carbonyl]-3- phenyl-2- quinoxalinepentanoic acid
550


 


118




7-[[1,2-dihydro-1- (methylsulfonyl)spiro[3H- indole-3,4′-piperidin]-1′- yl]carbonyl]-3-phenyl-2- quinoxalinepentanoic acid
599


 


119




7-[(1,2-dihydro-l-methyl-2- oxospiro 3H-indole-3,4′- piperidin]-1′-yl)carbonyl]-3- phenyl-2- quinoxalinepentanoic acid
549


 


120




3-phenyl-7-(spiro[benzofuran- 3(2H),4′-piperidin]-1′- ylcarbonyl)-2- quinoxalinepentanoic acid
522


 


121




7- [(2,3-dihydrospiro [1H- indene-1,4′-piperidin]-1′- yl)carbonyl]-3-phenyl-2- quinoxalinepentanoic acid
520


 


122




7-[(1,2-dihydro-1- methylspiro[3H-indole-3,4′- piperidin]-1′-yl)carbonyl]-3- phenyl-2- quinoxalinepentanoic acid
535


 


123




7-[(1-acetyl-1,2- dihydrospiro[3H-indole-3,4′- piperidin]-1′-yl)carbonyl]-3- phenyl-2- quinoxalinepentanoic acid
563


 


124




7-[(4-cyano-4-phenyl-1- piperidinyl)carbonyl]-3- phenyl-2- quinolaxinepentanoic acid
519


 


125




7- [(4-oxo-l-phenyl-1,3,8- triazaspiro[4.5]dec-8- yl)carbonyl]-3-phenyl-2- quinoxalinepentanoic acid
564


 


126




3-(4-fluorophenyl)-7-[(4-oxo- 1-phenyl-1,3,8- triazaspiro[4.5]dec-8- yl)carbonyl]-2- quinoxalinepentanoic acid
582


 


127




5-(3-(4-fluorophenyl)-7-(4- phenyl-1,2,3,6- tetrahydropyridine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
510


 


128




7-[(4-cyano-4-phenyl-1- piperidinyecarbonyl]-3-(4- fluorophenyl)-2- quinoxalinepentanoic acid
537


 


129




3-(4-fluorophenyl)-7- [[(phenylmethyl)amino] carbonyl]-2- quinoxalinepentanoic acid
458


 


130




7-[[[1(R)-(4- chlorophenyl)ethyl]amino] carbonyl]-3-(4-fluorophenyl)-2- quinoxalinepentanoic acid
506


 


131




7-[[[1(S)-(4- chlorophenyl)ethyl]amino] carbonyl]-3-(4-fluorophenyl)-2- quinoxalinepentanoic acid
506


 


132




3-(4-fluorophenyl)-7-[[(1(R)- phenylethyl)amino]carbonyl]- 2-quinoxalinepentanoic acid
472


 


133




3-(4-fluorophenyl)-7-[[(1(S)- phenylethyl)amino]carbonyl]- 2-quinoxalinepentanoic acid
472


 


134




7-[[4-(4-chlorophenyl)-4- hydroxy-1- piperidinyl]carbonyl]-3 -(4- fluorophenyl)-2- quinoxalinepentanoic acid
562


 


135




7-[[4-(4-chlorophenyl)-1- piperidinyl carbonyl]-3-(4- fluorophenyl)-2- quinoxalinepentanoic acid
546


 


136




7-[[4-(4-chlorophenyl)-3,6- dihydro-1(2H)- pyridinyl]carbonyl]-3-(4- fluorophenyl)-2- quinoxalinepentanoic acid
544


 


137




3-(4-fluorophenyl)-7-[[[2,2,2-2- trifluoro-1(R)- phenylethyl)amino]carbonyl]- 2-quinoxalinepentanoic acid
526


 


138




3-(4-fluorophenyl)-7-[[[2,2,2- trifluoro-1(S)-(4- fluorophenyl)ethyl]amino] carbonyl]-2- quinoxalinepentanoic acid
544


 


139




3-(4-fluorophenyl)-7-[[[(1R)- 2,2,2-trifluoro-1-(4- fluorophenyl)ethyl]amino] carbonyl]-2- quinoxalinepentanoic acid
544


 


140




3-phenyl-7-[(3(S)-phenyl-1- pyrrolidinyl)carbonyl]-2- quinoxalinepentanoic acid
480


 


141




3-phenyl-7-[(3(R)-phenyl-1- pyyrolidinyl)carbonyl]-2- quinoxalinepentanoic acid
480


 


142




3-phenyl-7-[(3(S)-phenyl-1- piperidinyl)carbonyl]-2- quinoxalinepentanoic acid
494


 


143




3-phenyl-7-[(3(R)-phenyl-1- piperidinyl)carbonyl]-2- quinooxalinepentanoic acid
494


 


144




5-(7-carbamoyl-3- phenylquinoxalin-2- yl)pentanoic acid
350


 


145




5-(7-(4-(1H-imidazol-1- yl)piperidine-l-carbonyl)-3- phenylquinoxalin-2- yl)pentanoic acid
484


 


146




7-[[[1(S)-(2- pyridinyl)ethyl]amino] carbonyl]-3-[4- (trifluoromethyl)phenyl]-2- quinoxalinepentanoic acid
523


 


147




7-[[[1(R)-(2- pyridinyl)ethyl]amino] carbonyl]-3-[4- (trifluoromethyl)phenyl]-2- quinoxalinepentanoic acid
523


 


148




7-[(4-phenyl-1- piperidinyl)carbonyl]-3-[4- (trifluoromethyl)phenyl]-2- quinoxalinepentanoic acid
562


 


149




3-[4-(trifluoromethyl)phenyl]- 7-[[(2,2,2-trifluoro-1(S)- phenylethyl)amino]carbonyl]- 2-quinoxalinepentanoic acid
576


 


150




7-[[(2,3-dihydro-1H-inden-2- yl)amino]carbonyl]-3-[4- (trifluoromethyl)phenyl]-2- quinoxalinepentanoic acid
534


 


151




7-[[[(3- chlorophenyl)methyl]amino] carbonly]-3-[4- (trifluoromethyl)phenyl]-2- quinoxalinepentanoic acid
542


 


152




7-[(3R)-phenyl-1- pyrrolidinyl)carbonyl]-3-  [4-(trifluoromethyl)phenyl]-2- quinoxalinepentanoic acid
548


 


153




7-[(3(S)-phenyl-1- pyrrolidinyl]carbonyl]-3-[4- (trifluoromethyl)phenyl]-2- quinoxalinepentanoic acid
548


 


154




7-[(3-phenyl-1- azetidinyl)carbonyl]-3-[4- (trifluoromethyl)phenyl]-2- quinoxalinepentanoic acid
534


 


155




7-[[[(4- chlorophenyl)methyl]amino] carbonyl]-3-[4- (trifluoromethyl)phenyl]-2- quinoxalinepentanoic acid
542


 


156




7-[[[[4- (trifluoromethoxy)phenyl] methyl]amino]carbonyl]-3-[4- (trifluoromethyl)phenyl]-2- quinoxalinepentanoic acid
592


 


157




7-[[[(4- cyanophenyl)methyl]amino] carbonyl]-3-[4- (trifluoromethyl)phenyl]-2- quinoxalinepentanoic acid
533


 


158




4-[2-[3-(4-chlorophenyl)-7- [(4-phenyl-1- piperidinyl)carbonyl]-2- quinoxalinyl]ethyl] benzeneacetic acid
590


 


159




4-[2-[3-(4-chlorophenyl)-7- [[[(3- chlorophenyl)methyl]amino] carbonyl]-2- quinoxalinyl]ethyl] benzeneacetic acid
570


 


160




3-(4-chlorophenyl)-7-[[[(3- fluorophenyl)methyl]amino] carbonyl]-alpha-methyl-2- quinoxalinepentanoic acid
506


 


161




3-(4-chlorophenyl)-7-[[[(4- fluorophenyl)methyl]amino] carbonyl]-alpha-methyl-2- quinoxalinepentanoic acid
506


 


162




3-(4-chlorophenyl)-alpha- methyl-7-[(4-phenyl-1- piperidinyl)carbonyl]-2- quinoxalinepentanoic acid
542


 


163




3-(4-chlorophenyl)-alpha- methyl-7-[[(2,2,2-trifluoro- 1(S)- phenylethyl)amino] carbonyl]-2- 2-quinoxalinepentanoic acid
556


 


164




3-(4-chlorophenyl)-7-[[[(4- cyanophenyl)methyl]amino] carbonyl]-alpha-methyl-2- quinoxalinepentanoic acid
513


 


165




3-(4-chlorophenyl)-alpha- methyl-7-[(3-phenyl-1- azetidinyl)carbonyl]-2- quinoxalinepentanoic acid
514


 


166




3(4-chlorophenyl)-7-[[[1(R)- (4-fluorophenyl)ethyl] amino]carbonyl]-alpha- methyl-2- quinoxalinepentanoic acid
520


 


167




3-(4-chlorophenyl)-7-[[[1(S)- (4-fluorophenyl)ethyl] amino]carbonyl]-alpha- methyl-2- quinoxalinepentanoic acid
520


 


168




3-(4-chlorophenyl)-7-[[(2,3- dihydro-1H-inden-2- yl)amino]carbonyl]-alpha- methyl-2- quinoxalinepentanoic acid
514


 


169




3-(4-chlorophenyl)-alpha- methyl-7-[(4-phenyl-1- piperazinyl)carbonyl]-2- quinoxalinepentanoic acid
543


 


170*




7-[(3(R)-methyl-3-phenyl-1- piperidinyl)carbonyl]-3- phenyl-2- quinoxalinepentanoic acid
508


 


171*




7-[(3(S)-methyl-3-phenyl-1- piperidinyl)carbonyl]-3- phenyl-2- quinoxalinepentanoic acid
508


 


172




3-phenyl-7-[(3-phenyl-1- azetidinyl)carbonyl]-2- quinoxalinepentanoic acid
466


 


173




3-(4-fluorophenyl)--7-[(3- phenyl-1- azetidinyl)carbonyl]-2- quinoxalinepentanoic acid
484


 


174




7-[[3-(4-cyanophenyl)-1- azetidinyl]carbonyl]-3- phenyl-2- quinoxalinepentanoic acid
491


 


175




7-[[3-(3-cyanophenyl)-1- azetidinyl]carbonyl]-3-phenyl-2- quinoxalinepentanoic acid
491


 


176




3-(4-chlorophenyl)-7-[(3- phenyl-1- piperidinyl)carbonyl]-2- quinoxalinepentanoic acid
528


 


177




3-(4-chlorophenyl)-7-(4- morpholinylcarbonyl)-2- quinoxalinepentanoic acid
454


 


178




3-(4-chlorophenyl)-7-[[[1(R)- (3-fluorophenyl)ethyl] amino]carbonyl]-2- quinoxalinepentanoic acid
506


 


179




3-(4-chlorophenyl)-7-[[[1(S)- (3-fluorophenyl)ethyl] amino]carbony!]-2- quinoxalinepentanoic acid
506


 


180




3-(4-chlorophenyl)-7-[[[1(R)- (3-chlorophenyl)ethyl] amino]carbonyl]-2- quinoxalinepentanoic acid
522


 


181




3-(4-chlorophenyl)-7-[[[1(S)- (3-chlorophenyl)ethyl] amino]carbonyl]-2- quinoxalinepentanoic acid
522


 


182




3-(4-chlorophenyl)-7-[[[1(R)- (3-pyridinyl)ethyl] amino]carbonyl]- 2-quinoxalinepentanoic acid
489


 


183




3-(4-chlorophenyl)-7-[[[1(S)- pyridinyl)ethyl] amino]carbonyl]-2- quinoxalinepentanoic acid
489


 


184




3-(4-chlorophenyl)-7-[[(6- fluoro-1,2,3,4-tetrahydro- 1(R)-naphthalenyl)amino] carbonyl]-2- quinoxalinepentanoic acid
532


 


185




3-(4-chlorophenyl)-7-[[(6- fluoro-1,2,3,4-tetrahydro- 1(S)-naphthalenyl)amino] carbonyl]2- quinoxalinepentanoic acid
532


 


186




3-(4-chlorophenyl)-7-[[(6- chloro-1,2,3,4-tetrahydro- 1(R)-naphthalenyl)amino] carbonyl]-2- quinoxalinepentanoic acid
548


 


187




3-(4-chlorophenyl)-7-[[(6- chloro-1,2,3,4-tetrahydro- 1(S)-naphthalenyl)amino] carbonyl]-2- quinoxalinepentanoic acid
548


 


188




3-(4-chlorophenyl)-7- [[[1(R)-(4-fluorophenyl)ethyl] methylamino] carbonyl]-2- quinoxalinepentanoic acid
520


 


189




3-(4-chlorophenyl)-7-[[4-(4- fluorophenyl)-1- piperazinyl]carbonyl]-2- quinoxalinepentanoic acid
547


 


190




3-(4-chlorophenyl)-7-[[4-(2- fluorophenyl)-1- piperazinyl]carbonyl]-2- quinoxalinepentanoic acid
547


 


191




3-(4-chlorophenyl)-7-[[4-(4- chlorophenyl)-1- piperazinyl]carbonyl]-2- quinoxalinepentanoic acid
563


 


192




3-[2-[3-(4-fluorophenyl)-7- [[[(3-fluorophenyl)methyl] amino]carbonyl]-2- quinoxalinyl] ethyl]benzencacetic acid
538


 


193




3-[2-[3-(4-fluorophenyl)-7- [[[(4-fluorophenyl)methyl] amino]carbonyl]-2- quinoxalinyl]ethyl] benzeneacetic acid
538


 


194




3-[2-[7-[[[(4- cyanophenyl]methyl]amino] carbonyl]-3-(4-fluorophenyl- 2-quinoxalinyl]ethyl] benzeneacetic acid
545


 


195




3-[2-[3-(4-fluorophenyl)-7- [[[1(R)-(4-fluorophenyl)ethyl] amino]carbonyl]-2- quinoxalinyl]ethyl] benzeneacetic acid
552


 


196




3-[2-[3-(4-fluorophenyl)-7- [[[1(S)-(4-fluorophenyl)ethyl] amino]carbonyl]-2- quinoxalinyl] ethylbenzeneacetic acid
552


 


197




3-[2-[3-(4-fluorophenyl)-7- [[(3-hydroxy-1(S)- phenylpropyl)amino] carbonyl]-2- quinoxalinyl]ethyl] benzeneacetic acid
564


 


198 




3-[2-[3-(4-fluorophenyl)-7- [(4-phenyl-1- piperidinyl)carbonyl]-2- quinoxalinyl]ethyl] benzeneacetic acid
574


 


199




3-[2-[3-(4-fluorophenyl)-7- [(4-phenyl-1- piperazinyl)carbonyl]-2- quinoxalinyl]ethyl] benzeneacetic acid
575


 


200




3-[2-[3-(4-fluorophenyl)-7- [(3-phenyl-1- azetidinyl)carbonyl]-2- quinoxalinyl]ethyl] benzeneacetic acid
546


 


201




3-[2-[3-(3-fluorophenyl)-7- [[[4-fluorophenyl)methyl] methylamino]carbonyl]- 2-quinoxalinyl]ethyl] benzeneacetic acid
552


 


202




3-[2-[3-(4-fluorophenyl)-7- [[methyl(phenylmethyl)amino] carbonyl]-2- quinoxalinyl]ethyl] benzeneacetic acid
534


 


203




3-(4-fluorophenyl)-7-[[[(3- fluorophenyl)methyl] amino]carbonyl]- alpha-methyl-2- quinoxalinepentanoic acid
490


 


204




3-(4-fluorophenyl)-7-[[[(4- fluorophenyl)methyl] amino]carbonyl]- alpha-methyl-2- quinoxalinepentanoic acid
490


 


205




3-(4-fluorophenyl)-alpha- methyl-7-[(4-phenyl-1- piperidinyl)carbonyl]-2- quinoxalinepentanoic acid
526


 


206




3-(4-fluorophenyl)-alpha- methyl-7-[(4-phenyl-1- piperazinyl)carbonyl]-2- quinoxalinepentanoic acid
527


 


207




7-[[[(4-cyanophenyl)methyl] amino]carbonyl]-3- (4-fluorophenyl)- alpha-methyl-2- quinoxalinepentanoic acid
497


 


208




3-(4-fluorophenyl)-alpha- methyl-7-[(3-phenyl-1- azetidinyl)carbonyl]-2- quinoxalinepentanoic acid
498


 


209




3-(4-fluorophenyl)-7-[[[1(R)- (4-fluorophenyl)ethyl] amino]carbonyl]- alpha-methyl-2- quinoxalinepentanoic acid
504


 


210




3-(4-fluorophenyl)-7-[[[1(S)- (4-fluorophenyl)ethyl] amino]carbonyl]-alpha- methyl-2- quinoxalinepentanoic acid
504


 


211




3-(4-fluorophenyl)-7-[[[(3- fluorophenyl)methyl] methylamino] carbonyl]-alpha-methyl- 2-quinoxalinepentanoic acid
504


 


212




3-(4-fluorophenyl)-alpha- methyl-7- [[methyl(phenylmethyl)amino] carbonyl]-2- quinoxalinepentanoic acid
486


 


213




3-(4-fluorophenyl)-7-[[(3- hydroxy-1(S)- phenylpropyl)amino] carbonyl]-alpha-methyl-2- quinoxalinepentanoic acid
516


 


214




7-[[[1(R)-(4- fluorophenyl)ethyl]amino] carbonyl]-3-(4-methoxyphenyl)- 2-quinoxalinepentanoic acid
502


 


215




3-(4-cyanophenyl)-7-[[[1(R)- (4-fluorophenyl)ethyl]amino] carbonyl]-2- quinoxalinepentanoic acid
497


 


216




3-[4-(1-methyl- ethoxy)phenyl]-7-[(4- phenyl-1- piperidinyl)carbonyl]-2- quinoxalinepentanoic acid
552


 


217




3-(4-fluorophenyl)-7- [[(1,2,3,4-tetrahydro-1(R)- naphthalenyl)amino] carbonyl]- 2-quinoxalinepentanoic acid
498


 


218




3-(4-fluorophenyl)-7- [[(1,2,3,4-tetrahydro-1(S)- naphthalenyl)amino] carbonyl]-2- quinoxalinepentanoic acid
498


 


219




3-(4-fluorophenyl)-7-[[(6- fluoro-1,2,3,4-tetrahydro- 1(R)-naphthalenyl)amino] carbonyl]-2- quinoxalinepentanoic acid
516


 


220




3-(4-fluorophenyl)-7-[[(6- fluoro-1,2,3,4-tetrahydro- 1(S)-naphthalenyl)amino] carbonyl]-2- quinoxalinepentanoic acid
516


 


221




7-[[(6-chloro-1,2,3,4- tetrahydro-1(R)- naphthalenyl)amino] carbonyl]-3-(4-fluorophenyl)-2- quinoxalinepentanoic acid
532


 


222




7-[[(6-chloro-1,2,3,4- tetrahydro-1(S)- naphthalenyl)amino]carbonyl]- 3-(4-fluorophenyl)-2- quinoxalinepentanoic acid
532


 


223




7-[[(5-fluoro-2,3-dihydro-1H- inden-1(R)- yl)amino]carbonyl]-3-(4- fluorophenyl)-2- quinoxalinepentanoic acid
502


 


224




7-[[(5-fluoro-2,3-dihydro-1H- inden-1(5)- yl)amino]carbonyl]-3-(4- fluorophenyl)-2- quinoxalinepentanoic acid
502


 


225




7-[[(5-chloro-2,3-dihydro-1H- inden-1(R)- yl)amino]carbonyl]-3-(4- fluorophenyl)-2- quinoxalinepentanoic acid
518


 


226




7- [[(5-chloro-2,3-dihydro-1H- inden-1(S)- yl)amino]carbonyl]-3-(4- fluorophenyl)-2- quinoxalinepentanic acid
518


 


227




7-[[[1(S)-(4-chlorophenyl)- 2,2,2-trifluoroethyl] amino]carbonyl]-3- (4-fluorophenyl)-2- quinoxalinepentanoic acid
560


 


228




7-[[[1(R)-(4-chlorophenyl)- 2,2,2-trifluoroethyl] amino]carbonyl]-3- (4-fluorophenyl)-2- quinoxalinepentanoic acid
560


 


229




7-[[(2,3-dihydro-1H-inden- 1(R)-yl)amino]carbonyl]-3 - (4-fluorophenyl)-2- quinoxalinepentanoic acid
484


 


230




7- [[(2,3-dihydro-1H-inden- 1(S)-yl)amino]carbonyl]-3-(4- fluorophenyl)-2- quinoxalinepentanoic acid
484


 


231




3-(4-fluorophenyl)-7-[(4- phenyl-1- piperazinyl)carbonyl]-2- quinoxalinepentanoic acid
513


 


232




trans-2-[2-[7-[[[1(R)-(4- chlorophenyl)ethyl] amino]carbonyl]-3-phenyl-2- quinoxalinyl]ethyl] cyclopropanecarboxylic acid
500


 


233




trans-2-[2-[7-[[[1(R)-(4- chlorophenyl)ethyl]amino] carbonyl]-3- phenyl-2-quinexalinyl] ethyl]cyclopropanecarboxylic acid
500


 


234




7-[[[1(R)-(4- fluorophenyl)ethyl] amino]carbonyl]-3-phenyl-2- quinoxalinepentanoic acid
472


 


235




7-[[[1(S)-(4- fluorophenyl)ethyl]amino] carbonyl]-3-phenyl-2- quinoxalinepentanoic acid
472


 


236




7-[[[1(R)-(4- fluorophenyl)ethyl]methylamino] carbonyl]-3-phenyl-2- quinoxalinepentanoic acid
486


 


237




7-[[[1(S)-(4- fluorophenyl)ethyl]methylamino] carbonyl]-3-phenyl-2- quinoxalinepentanoic acid
486


 


238




7-[[[1(R)-(4- chlorophenyl)ethyl]methylamino] carbonyl]-3-phenyl-2- quinoxalinepentanoic acid
502


 


239




7-[[[1(S)-(4- chlorophenyl)ethyl]methylamino] carbonyl]-3-phenyl-2- quinoxalinepentanoic acid
502


 


240




3-(4-fluorophenyl)-7-[[[1(R)- (4-fluorophenyl)ethyl] methylamino] carbonyl]-2- quinoxalinepentanoic acid
504


 


241




3-(4-fluorophenyl)-7-[[[1(S)- (4-fluorophenyl)ethyl] methylamino] carbonyl]-2- quinoxalinepentanoic acid
504


 


242




7-[[[1(R)-(4- chlorophenyl)ethyl] methylamino]carbonyl]-3-(4- fluorophenyl)-2- quinoxalinepentanoic acid
520


 


243




7-[[[1(S)-(4- chlorophenyl)ethyl] methylamino]carbonyl]-3-(4- fluorophenyl)-2- quinoxalinepentanoic acid
520


 


244




3-(4-fluorophenyl)-7-[[4-(2- pyrimidinyl)-1- piperidinyl]carbonyl]-2- quinoxalinepentanoic acid
514


 


245




3-phenyl-7-[[4-(2- pyrimidinyl)-1- piperidinyl]carbonyl]-2- quinoxalinepentanoic acid
496


 


246




7- [[3-(4-cyanophenyl)-1- azetidinyl]carbonyl]-3-(4- fluorophenyl)-2- quinoxalinepentanoic acid
509


 


247




7-[[3-(1H-benzimidazol-2-yl)- 1-azetidinyl]carbonyl]-3- phenyl-2- quinoxalinepentanoic acid
506


 


248




7-[[3-(2-benzoxazolyl)-1- azetidinyl]carbonyl]-3-(4- fluorophenyl)-2- quinoxalinepentanoic acid
525


 


249




7-[[3-(3,4-difluorophenyl)-1- azetidinyl]carbonyl]-3- phenyl-2- quinoxalinepentanoic acid
502


 


250




7-[[3-(4-fluorophenyl)-1- azetidinyl]carbonyl]-3- phenyl-2- quinoxalinepentanoic acid
484


 


251




7-[[3-(3-fluorophenyl)-1- azetidinyl]carbonyl]-3- phenyl-2- quinoxalinepentanoic acid
484


 


252




7-[[3-(4-chlorophenyl)-1- azetidinyl]carbonyl]-3- phenyl-2- quinoxalinepentanoic acid
500


 


253




7-[[3-(3-chlorophenyl)-1- azetidinyl]carbonyl]-3- phenyl-2- quinoxalinepentanoic acid
500


 


254




7-[[(6-fluoro-1,2,3,4- tetrahydro-1(R)- naphthalenyl)amino]carbonyl]-3- phenyl-2- quinoxalinepentanoic acid
498


 


255




7-[[(6-chloro-1,2,3,4- tetrahydro-1(R)- naphthalenyl)amino)carbonyl]- 3-phenyl-2- quinoxalinepentanoic acid
514


 


256




(R)-5-(3-phenyl-7-((1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
480


 


257




(S)-5-(3-(4-ch1orophenyl)-7- ((1-(4-cyanophenyl)ethyl) carbamoyl)quinoxalin-2- pentanoic acid
513


 


258




(S)-5-(3-(4-fluorophenyl)-7- (((R)-1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)quinoxalin-2-yl)- 3-methylpentanoic acid
512


 


259




2-(3-(3-(4-chlorophenyl)-7-(4- phenylpiperazine-1- carbonyl)quinoxalin-2- yl)phenyl)acetic acid
563


 


260




(R)-2,2-difluoro-5-(7-((6- fluoro-1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)-3-(4- fluorophenyl)quinoxalin-2- yl)pentanoic acid
552


 


261




(1S,2S)-2-(2-(7-(((R)-6-fluoro- 1,2,3,4-tetrahydronaphthalen- 1-yl)carbamoyl)-3-(4- fluorophenyl)quinoxalin-2- yl)ethyl)cyclopropane- carboxylic acid
528


 


262




(1S,2S)-2-(2-(3-(4- fluorophenyl)-7-(((R)-1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)quinoxalin-2- yl)ethyl)cyclopropanecarboxylic acid
510


 


263




(1R,2R)-2-(2-(7-(((R)-6- fluoro-1,2,3,4- tetrahydronaphthalen-l- yl)carbamoyl)-3-(4- fluorophenyl)quinoxalin-2- yl)ethyl)cyclopropanecarboxylic acid
528


 


264




2-(2-(7-(((R)-6-fluoro-1,2,3,4- tetrahydronaphthalen-l- yl)carbamoyl)-3-(4- fluorophenyl)quinoxalin-2- yl)ethyl)cyclopropanecarboxylic acid
528


 


265




(R)-5-(7-(4-(4-fluorophenyl)- 2-methylpiperazine-1- carbonyl)-3-phenylquinoxalin- 2-yl)pentanoic acid
527


 


266




5-(7-((2-oxo-1,2,3,4- tetrahydroquinolin-4- yl)carbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
495


 


267




5-(3-(4-chlorophenyl)-7-(1- oxo-2,3-dihydro-1H- spiro[isoquinoline-4,4′- piperidin]-1′- ylcarbonyl)quinoxalin-2- yl)pentanoic acid
583.3


 


268




5-(3-(4-chlorophenyl)-7-((2- oxo-2,3,4,5-tetrahydro-1H- benzo[b]azepin-5- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
543.3


 


269




(R)-2-(3-(3-(3-(4- fluorophenyl)-7-((1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)quinoxalin-2- yl)propyl)phenyl)acetic acid
574.4


 


270




(S)-2-(3-(3-(3-(4- fluorophenyl)-7-((1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)quinoxalin-2- yl)propyl)phenyl)acetic acid
574.4


 


271




(R)-2-(3-(3-(3-(4- fluorophenyl)-7-((1-(4- fluorophenyl)ethyl)carbamoyl) quinoxalin-2- yl)propyl)phenyeacetic acid
566.3


 


272




2-(3-(3-(3-(4-fluorophenyl)-7- (4-phenylpiperazine-1- carbonyl)quinoxalin-2- yl)propyl)phenyl)acetic acid
589.4


 


273




2-(3-(3-(3-(4-fluorophenyl)-7- (3-(3-fluorophenyl)azetidine-1- carbonyl)quinoxalin-2- yl)propyl)phenyl)acetic acid
578.4


 


274




(S)-2-(3-(3-(3-(4- fluorophenyl)-7-(3- phenylpyrrolidine-1- carbonyl)quinoxalin-2- yl)propyl)phenyl)acetic acid
574.4


 


275




2-(3-(3-(7-((4- fluorobenzyl)(methyl)carbamoyl)- 3-(4-fluorophenyl)quinoxalin-2- yl)propyl)phenyl)acetic acid
566.4


 


276




(R)-3-methyl-5-(3-phenyl-7- (((R)-1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
494.4


 


277




(R)-3-methyl-5-(3-phenyl-7- (((S)-1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
494.4


 


278




(R)-5-(7-(((R)-1-(4- fluorophenyl)ethyl)carbamoyl)- 3-phenylquinoxalin-2-yl)-3- methylpentanoic acid
486.4


 


279




(R)-5-(7-(((S)-1-(4- fluorophenyl)ethyl)carbamoyl)- 3-phenylquinoxalin-2-yl)-3- methylpentanoic acid
486.4


 


280




(R)-3-methyl-5-(3-phenyl-7- (4-phenylpiperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
509.4


 


281




(R)-5-(7-(3-(3- fluorophenyl)azetidine-1- carbonyl)-3-phenylquinoxalin- 2-yl)-3-methylpentanoic acid
498.4


 


282




(R)-3-methyl-5-(3-phenyl-7- ((S)-3-phenylpyrrolidine-1- carbonyl)quinoxalin-2- yl)pentannic acid
494.4


 


283




(R)-5-(7-((4- fluorobenzyl)(methyl) carbamoyl)-3- phenylquinoxalin-2-yl)- 3-methylpentanoic acid
486.4


 


284




5-(7-((6-fluoro-2-oxo-1,2,3,4- tetrahydroquinolin-4- yl)carbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
513


 


285




5-(3-(4-chlorophenyl)-7- ((1R,5S)-1-(p-tolyl)-3 - azabicyclo[3.1.0]hexane-3- carbonyl)quinoxalin-2- yl)pentanoic acid
540.4


 


286




(S)-3-methyl-5-(3-phenyl-7- (((S)-1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
494.4


 


287




(S)-5-(7-(((R)-1-(4- fluorophenyl)ethyl) carbamoyl)-3- phenylquinoxalin-2-yl)-3- methylpentanoic acid
486.3


 


288




(S)-5-(7-(((S)-1-(4- fluorophenyl)ethyl)carbamoyl)-3- 3-phenylquinoxalin-2-yl)-3- methylpentanoic acid
486.3


 


289




(S)-3-methyl-5-(3-phenyl-7-(4- phenylpiperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
509.4


 


290




(S)-5-(7-(3-(3- fluorophenyl)azetidine-1- carbonyl)-3-phenylquinoxalin- 2-yl)-3-methylpentanoic acid
498.4


 


291




(S)-3-methyl-5-(3-phenyl-7- ((S)-3-phenylpyrrolidine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
494.4


 


292




(S)-5-(7-((4- fluorobenzyl(methyl)carbamoyl)-3- phenylquinoxalin-2-yl)- 3-methylpentanoic acid
486.3


 


293




(S)-3-methyl-S-(3-phenyl-7- (((R)-1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
494.4


 


294




5-(3-phenyl-7-((2,3,4,5- tetrahydrobenzo[b]oxepin-5- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
496.4


 


295




5-(7((2,2-dimethylchroman-4- yl)carbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
510.4


 


296




5-(7-((4,4-dimethyl-1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
508.4


 


297




5-(7-(3,4-dihydro-2H- spiro[naphthalene-1,3′- pyrrolidin]-1′-ylcarbonyl)-3- phenylquinoxalin-2- yl)pentanoic acid
520.4


 


298




5-(7-(i-oxo-2,3-dihydro-1H- spiro[isoquinoline-4,4′- piperidin]-1′-ylcarbonyl)-3- phenylquinoxalin-2- yl)pentanoic acid
549.4


 


299




5-(7-(3-oxo-3,4-dihydro-2H- spiro[isoquinoline-1,4′- piperidin]-1′-ylcarbonyl)-3- phenylquinoxalin-2- yl)pentanoic acid
549.4


 


300




5-(7-(1-oxo-2,4-dihydro-1H- spiro[isoquinoline-3,4′- piperidin]-1′-ylcarbonyl)-3- phenylquinoxalin-2- yl)pentanoic acid
549.4


 


301




(R)-5-(3-phenyl-7- (thiochroman-4- ylcarbamoyl)quinoxalin-2- yl)pentanoic acid
498.3


 


302




5-(7-(4-benzylpiperazine-1- carbonyl)-3-phenylquinoxalin- 2-yl)pentanoic acid
509.4


 


303




5-(7-(4-(3-cyanopyridin-2- yl)piperazine-1-carbonyl)-3- phenylquinoxalin-2- yl)pentanoic acid
521.4


 


304




5-(7-(4-(3,5-dichloropyridin-4- yl)piperazine-1-carbonyl)-3- phenylquinoxalin-2- yl)pentanoic acid
564.3


 


305




5-(3-phenyl-7-(4-(2- (trifluoromethyl)phenyl) piperazine- 1-carbonyl)quinoxalin-2- yl)pentanoic acid
563.3


 


306




5-(3-phenyl-7-(4-(thiazol-2- yl)piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
502.3


 


307




5-(7-(isoindoline-2-carbonyl)- 3-phenylquinoxalin-2- yl)pentanoic acid
452.3


 


308




5-(7-(4-(2- chlorophenyl)piperazine-1- carbonyl)-3-phenylquinoxalin- 2-yl)pentanoic acid
529.3


 


309




5-(3-phenyl-7-(4-(3- (trifluoromethyl)phenyl) piperazine-l-carbonyl) quinoxalin-2- yl)pentanoic acid
563.4


 


310




(S)-5-(7((7-fluorochroman-4- yl)carbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
500.3


 


311




5-(7-((6-(benzyloxy)-1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)-3-(4- chlorophenyl)quinoxalin-2- yl)pentanoic acid
620.6


 


312




5-(3-(4-chlorophenyl)-7((6- methoxy-1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
544.5


 


313




(R)-5-(3-(4-chlorophenyl)-7- ((6-fluoro-2,3- dihydrobenzofuran-3- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
520.4


 


314




(S)-5-(3-(4-chlorophenyl)-7- ((6-fluoro-2,3- dihydrobenzofuran-3- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
520.4


 


315




5-(3-(4-chlorophenyl)-7-((1- methyl-2-oxo-1,2,3,4- tetrahydroquinolin-4- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
543.3


 


316




5-(3-(4-chlorophenyl)-7-(4-(1- oxoisoindolin-2-yl)piperidine- 1-carbonyl)quinoxalin-2- yl)pentanoic acid
583.5


 


317




5-(3-(4-chlorophenyl)-7-(((1- (4- (trifluoromethoxy) phenyl)cyclopropyl) methyl)carbamoyl) quinoxalin-2-yl)pentanoic acid
598.5


 


318




5-(3-(4-chlorophenyl)-7-(((1- (4-fluorophenyl) cyclopropyl)methyl)carbamoyl) quinoxalin-2- yl)pentanoic acid
532.5


 


319




5-(3-(4-chlorophenyl)-7((3- oxoisoindolin-1- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
515.4


 


320




(R)-5-(3-(4-chlorophenyl)-7- ((1,1-dioxidothiochroman-4- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
564.4


 


321




(S)-5-(3-(4-chlorophenyl)-7- ((1-(isoquinolin-4- yl)ethyl)carbamoyl)quinoxalin- 2-yl)pentanoic acid
539.5


 


322




(S)-5-(3-(4-chlorophenyl)-7- ((1-(quinolin-5- yl)ethyl)carbamoyl)quinoxalin- 2-yl)pentanoic acid
539.5


 


323




5-(7-(3-(benzyloxy)azetidine- 1-carbonyl)-3-(4- chlorophenyl)quinoxalin-2- yl)pentanoic acid
530.5


 


324




5-(7-(3-(1H-pyrazol-1- yl)azetidine-1-carbonyl)-3 -(4- chlorophenyl)quinoxalin-2- yl)pentanoic acid
490.4


 


325




5-(7-(3-(1H-imidazol-1- yl)azetidine-1-carbonyl)-3-(4- chlorophenyl)quinoxalin-2- yl)pentanoic acid
490.4


 


326




5-(3-(4-chlorophenyl)-7-(3-(3- (pyridin-4-yl)-1,2,4-oxadiazol- 5-yl)azetidine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
569.5


 


327




5-(7-(3-(4H-1,2,4-triazol-4- yl)azetidine-l-carbonyl)-3-(4- chlorophenyl)quinoxalin-2- yl)pentanoic acid
491.4


 


328




5-(3-(4-chlorophenyl)-7-(3- fluoro-3-phenylazetidine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
518.4


 


329




5-(3-(4-chlorophenyl)-7-(3- (pyridin-2-yloxy)azetidine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
517.5


 


330




(R)-5-(3-(3- (dimethylamino)phenyl)-7- ((1,2,3,4-tetrahydronaphthalen- 1-yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
523.4


 


331




5-(3-(4-chlorophenyl)-7-((2,3- dihydrobenzo[b]thiophen-3- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
518.2


 


332




(R)-5-(3-(4-chlorophenyl)-7- ((7-cyanochroman-4- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
541.2


 


333




(S)-5-(3-(4-chlorophenyl)-7- ((7-cyanochroman-4- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid.
541.2


 


334




(R)-5-(7-((7- chlorochroman-4- yl)carbamoyl)-3-(4- chlorophenyl)quinoxalin-2- yl)pentanoic acid
550.2


 


335




(S)-5-(7-((7-chlorochroman-4- yl)carbamoyl)-3-(4- chlorophenyl)quinoxalin-2- yl)pentanoic acid
550.2


 


336




5-(3-(4-chlorophenyl)-7- (methyl(3-oxoisoindolin-1- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
529.2


 


337




5-(7-([1,4′-bipiperidine]-1′- carbonyl)-3 (4- chlorophenyl)quinoxalin-2- yl)pentanoic acid
535.3


 


338




5-(7-(4-(1H-indol-1- yl)piperidine-l-carbonyl)-3-(4- chlorophenyl)quinoxalin-2- yl)pentanoic acid
567.3


 


339




5-(3-(4-chlorophenyl)-7-(4- (pyrrolidin-1-yl)piperidine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
521.3


 


340




5-(3-(4-chlorophenyl)-7-(2- oxa-8-azaspiro[4.5]decane-8- carbonyl)quinoxalin-2- yl)pentanoic acid
508.3


 


341




5-(7-(4-(1H-1,2,4-triazol- yl)piperidine-1-carbonyl)-3-(4- chlorophenyl)quinoxalin-2- yl)pentanoic acid
519.3


 


342




5-(7-(4-(1H-pyrazol-1- yl)piperidine-1-carbonyl)-3-(4- chlorophenyl)quinoxalin-2- yl)pentanoic acid
518.3


 


343




5-(3--(4-chlorophenyl)-7-(2H- spiro[benzofuran-3,4′- piperidin]-1′- ylcarbonyl)quinoxalin-2- yl)pentanoic acid
556.3


 


344




5-(3-(4-chlorophenyl)-7-(4- oxo-3,4- dihydrospiro[benzo[e][1,3] oxazine-2,4′-piperidin]-1′- ylcarbonyl)quinoxalin-2- yl)pentanoic acid
585.3


 


345




5-(3-(4-chlorophenyl)-7- ((1R,5S,9r)-9-phenyl-3- azabicyclo[3.3.1]nonane-3- carbonyl)quinoxa1in-2- yl)pentanoic acid
568.3


 


346




5-(3-(4-chlorophenyl)-7-(4-(1- methyl-1H-imidazol-4- yl)piperidine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
532.3


 


347




5-(3-(4-chlorophenyl)-7-(2- cyclopropyl-4,5,6,7- tetrahydrooxazolo[4,5- c]pyridine-5- carbonyl)quinoxalin-2- yl)pentanoic acid
531.2


 


348




5-(7-(4-(1H-indazol-1- yl)piperidine-1-carbonyl)-3-(4- chlorophenyl)quinoxalin-2- yl)pentanoic acid
568.3


 


349




5-(3-(4-chlorophenyl)-7-(2- methyl-3-oxo-2,8- diazaspiro[4.5]decane-8- carbonyl)quinoxalin-2- yl)pentanoic acid
535.3


 


350




5-(7-(3-oxo-4- phenylpiperazine-1-carbonyl)- 3-phenylquinoxalin-2- yl)pentanoic acid
509.3


 


351




5-(7-(4-(4- fluorophenyl)piperazine-1- carbonyl)-3-phenylquinoxalin- 2-yl)pentanoic acid
513.3


 


352




5-(3-(4-fluorophenyl)-7-((2- oxo-1,2,3,4- tetrahydroquinolin-4- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
513.3


 


353




5-(3-(4-fluorophenyl)-7-(1- oxo-2,3-dihydro-1H- spiro[isoquinoline-4,4′- piperidin]-1′- ylcarbonyl)quinoxalin-2- yl)pentanoic acid
567.3


 


354




(R)-5-(3-(4-fluorophenyl)-7- ((5,6,7,8- tetrahydroisoquinolin-8- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
499.3


 


355




(R)-5-(3-(4-fluorophenyl)-7- (thiochroman-4- ylcarbamoyl)quinoxalin-2- yl)pentanoic acid
516.3


 


356




5-(3-(4-fluorophenyl)-7-(4-(2- oxo-2,3-dihydro-1H- benzo[d]imidazol-1- yl)piperidine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
568.3


 


357




5-(3-(4-fluorophenyl)-7-(4- (pyridin-2-yl)piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
514.3


 


358




5-(7-(4-(3-cyanopyridin-2- yl)piperazine-l-carbonyl)-3-(4- fluorophenyl)quinoxalin-2- yl)pentanoic acid
539.3


 


359




5-(7-(4-(2- cyanophenyl)piperazine-1- carbonyl)-3-(4- fluorophenyl)quinoxalin-2- yl)pentanoic acid
538.3


 


360




5-(3-(4-fluorophenyl) 7-(4- (pyrazin-2-yl)piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
515.3


 


361




5-(3-(4-fluorophenyl)-7-(4-(2- methoxyphenyl)piperidine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
542.3


 


362




5-(3-(4-fluorophenyl)-7-(3H- spiro[isobenzofuran-1,4′- piperidin]-1′-ylcarbonyl) quinoxalin-2- yl)pentanoic acid
540.3


 


363




5-(3-(4-fluorophenyl)-7-(4-(2- (trifluoromethyl) phenyl)piperazine- 1-carbonyl)quinoxalin-2- yl)pentanoic acid
581.3


 


364




5-(7-(4-(2- chlorophenyl)piperazine-1- carbonyl)-3-(4- fluorophenyl)quinoxalin-2- yl)pentanoic acid
547.3


 


365




5-(3-(4-fluorophenyl)-7-(4-(o- tolyl)piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
527.3


 


366




5-(3-(4-fluorophenyl)-7-(4-(m- tolyl)piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
527.3


 


367




5-(3-(4-fluorophenyl)-7-(4-(4- methoxyphenyl)piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
543.3


 


368




5-(3-(4-fluorophenyl)-7-(4-(3- methylpyridin-4-yl)piperazine- 1-carbonyl)quinoxalin-2- yl)pentanoic acid
528.3


 


369




5-(3-(4-fluorophenyl)-7-(4-(p- tolyl)piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
527.3


 


370




(R)-8-(3-(4-carboxybutyl)-2- phenylquinoxaline-6- carboxamido)-5,6,7,8- tetrahydroquinolin-1-ium 2,2,2-trifluoroacetic acid
481


 


371




(S)-8-(3-(4-carboxybutyl)-2- phenylquinoxaline-6- carboxamido)-5,6,7,8- tetrahydroquinolin-1-ium 2,2,2-trifluoroacetic acid
481


 


372




5-(3-(4-fluorophenyl)-7-(3-(4- methoxyphenyl)azetidine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
514


 


373




5-(3-(4-fluorophenyl)-7-(3-(3- methoxyphenyl)azetidine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
514


 


374




(R)-5-(3-(4-chlorophenyl)-7- ((1-(3,4- difluorophenyl)ethyl)carbamoyl) quinoxalin-2-yl)pentanoic acid
524


 


375




(S)-5-(3-(4-chlorophenyl)-7- ((1-(3,4- difluorophenyl)ethyl)carbamoyl) quinoxalin-2-yl)pentanoic acid
524


 


376




(R)-5-(3-(4-chlorophenyl)-7- ((1-(4- fluorophenyl)propyl)carbamoyl) quinoxalin-2-yl)pentanoic acid
522


 


377




(S)-5-(3-(4-chlorophenyl)-7- ((1-4- fluorophenyl)propyl)carbamoyl) quinoxalin-2-yl)pentanoic acid
522


 


378




(R)-5-(7-(methyl(1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
494


 


379




(R)-5-(7-((6-fluoro-1,2,3,4- tetrahydronaphthalen-1- yl)(methyl)carbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
512


 


380




(R)-5-(7-((6-chloro-1,2,3,4- tetrahydronaphthalen-1- yl)(methyl)carbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
528


 


381




(R)-5-(3-(4-chlorophenyl)-7- ((5-fluoro-2,3-dihydro-1H- inden-1-yl)carbamoyl) quinoxalin-2- yl)pentanoic acid
518


 


382




(S)-5-(3-(4-chlorophenyl)-7- ((5-fluoro-2,3-dihydro-1H- inden-1- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
518


 


383




(R)-5-(7-((5-chloro-2,3- dihydro-1H-inden-1- yl)carbamoyl)-3-(4- chlorophenyl)quinoxalin-2- yl)pentanoic acid
534


 


384




(S)-5-(7-((5-chloro-2,3- dihydro-1H-inden-1- yl)carbamoyl)-3-(4- chlorophenyl)quinoxalin-2- yl)pentanoic acid
534


 


385




(S)-5-(7-((6-fluoro-1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
498


 


386




(R)-5-(7-((6-chloro-1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
514


 


387




(S)-5-(7-((6-chloro-1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
514


 


388




(S)-5-(3-phenyl-7-((1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
480


 


389




5-(7-(3-(4- methoxyphenyl)azetidine-1- carbonyl)-3-phenylquinoxalin- 2-yl)pentanoic acid
496


 


390




5-(7-(3-(3- methoxyphenyl)azetidine-1- carbonyl)-3-phenylquinoxalin- 2-yl)pentanoic acid
496


 


391




5-(3-(4-chlorophenyl)- 7-(((R)- 1-(4-chlorophenyl) ethyl)carbamoyl) quinoxalin-2-yl)-2- methylpentanoic acid
536


 


392




5-(3-(4-chlorophenyl)-7-(((S)- 1-(4-chlorophenyl) ethyl)carbamoyl) quinoxalin-2-yl)-2-  methylpentanoic acid
536


 


393




3-((3-(4-chlorophenyl)-7-((4- methylbenzyl)carbamoyl) quinoxalin-2- yl)methyl)cyclopentane- carboxylic acid



 


394




3-((3-(4-chlorophenyl)-7-(((R)- 1-phenylethyl)carbamoyl) quinoxalin-2- yl)methyl)cyclopentane- carboxylic acid
514


 


395




3-((3-(4-chlorophenyl)-7-(((R)- 2,2,2-trifluoro-1- phenylethyl)carbamoyl) quinoxalin-2- yl)methyl)cyclopentane- carboxylic acid
568


 


396




3-((3-(4-chlorophenyl)-7-(3-(4- fluorophenyl)azetidine-1- carbonyl)quinoxalin-2- yl)methyl)cyclopentanecarboxylic acid
544


 


397




3-((3-(4-chlorophenyl)-7-(3-(3- fluorophenyl)azetidine-1- carbonyl)quinoxalin-2- yl)methyl)cyclopentane- carboxylic acid
544


 


398




3-((3-(4-chlorophenyl)-7-(3- (3,4-difluorophenyl)azetidine- 1-carbonyl)quinoxalin-2- yl)methyl)cyclopentane- carboxylic acid
562


 


399




(R)-5-(3-(4-chlorophenyl)-7- ((1-(4-fluorophenyl)-2- methylpropyl)carbamoyl) quinoxalin-2-yl) pentanoic acid
534


 


400




3-((3-(4-chlorophenyl)-7-(3-(4- cyanophenyl)azetidine-1- carbonyl)quinoxalin-2- yl)methyl)cyclopentane- carboxylic acid



 


401




3-((3-(4-chlorophenyl)-7-(4R)- 1-(4-chlorophenyl)-2,2,2- trifluoroethyl)carbamoyl) quinoxalin-2- yl)methyl)cyclopentane- carboxylic acid
603


 


402




(S)-5-(3-(4-chlorophenyl)-7- ((1-(4-fluorophenyl)-2- methylpropyl)carbamoyl) quinoxalin-2-yl) pentanoic acid
534


 


403




(R)-5-(3-(4-chlorophenyl)-7- ((1-(4- cyanophenyl)ethyl)carbamoyl) quinoxalin-2-yl)pentanoic acid
513


 


404




3-((3-(4-chlorophenyl)-7-(((S)- 1-phenylethyl)carbamoyl) quinoxalin-2- yl)methyl)cyclopentane- carboxylic acid
514


 


405




3-((3-(4-chlorophenyl)-7-(((S)- 2,2,2-trifluoro-1- phenylethyl)carbamoyl) quinoxalin-2- yl)methyl)cyclopentane- carboxylic acid
568


 


406




3-((3-(4-chlorophenyl)-7-(((S)- 1-(4-chlorophenyl)-2,2,2- trifluoroethyl)carbamoyl) quinoxalin-2- yl)methyl)cyclopentane- carboxylic acid
603


 


407




3-((3-(4-chlorophenyl)-7-(((S)- 1-(4-fluorophenyl)ethyl) carbamoyl)quinoxalin-2- yl)methyl)cyclopentane- carboxylic acid
532


 


408




5-(3-(4-chlorophenyl)- 7-(((R)-1-(4- fluorophenyl)ethyl)carbamoyl) quinoxalin-2-yl)-2- methylpentanoic acid
520


 


409




5-(3-(4-chlorophenyl)-7-(((R)- 1-(4-fluorophenyl)ethyl) carbamoyl)quinoxalin-2-yl)-2- methylpentanoic acid
520


 


410




(R)-5-(3-(3-cyanophenyl)-7- ((1 -(4- fluorophenyl)ethyl)carbamoyl) quinoxalin-2-yl)pentanoic acid
497


 


411




(R)-5-(3-(3,4-difluarophenyl)- 7-(((1-(4-fluorophenyl) ethyl)carbamoyl) quinoxalin-2-yl)pentanoic acid
508


 


412




(R)-5-(3-(4-chlorophenyl)-7- ((1-(3,5-difluorophenyl) ethyl)carbamoyl) quinoxalin-2-yl) pentanoic acid
524


 


413




(S)-5-(3-(4-chlorophenyl)-7- ((1-(3,5-difluorophenyl)ethyl) carbamoyl)quinoxalin-2-yl) pentanoic acid
524


 


414




5-(3-(4-chlorophenyl)-7-(4-(4- fluorophenyl)piperidine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
546


 


415




5-(3-(4-chlorophenyl)-7-(4-(4- chlorophenyl)piperidine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
562


 


416




(R)-5-(3-(2,4-dichlorophenyl)- 7-((1-(4-fluorophenyl)ethyl) carbamoyl)quinoxalin-2- yl)pentanoic acid
540


 


417




(R)-5-(3-(2-chloro-4- (trifluoromethyl)phenyl)- 7-((1-(4-fluorophenyl)ethyl) carbamoyl)quinoxalin-2-yl) pentanoic acid
574


 


418




(R)-3-(3-(4-chlorophenyl)-7- ((1-(4-fluorophenyl)ethyl) carbamoyl)quinoxalin-2-yl) propanoic acid
478


 


419




5-(3-(4-chlorophenyl)-7-(3-(3- fluorophenyl)azetidine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
518


 


420




5-(3-(4-chlorophenyl)-7-(3- (3,4-difluorophenyl)azetidine- 1-carbonyl)quinoxalin-2- yl)pentanoic acid
536


 


421




5-(3-(4-chlorophenyl)-7-(3-(4- cyanophenyl)azetidine-l- carbonyl)quinoxalin-2- yl)pentanoic acid
525


 


422




(R)-3-((7-((1-(4-fluorophenyl) ethyl)carbamoyl)-3- phenylquinoxalin-2- yl)methyl)cyclobutanecarboxyl acid
484


 


423




2-methyl-5-(7-(4- phenylpperazine-1-carbonyl)- 3-(4-(trifluoromethyl) phenyl)quinoxalin-2-yl) pentanoic acid
577


 


424




3-methyl-5-(7-(4- phenylpiperazine-1-carbonyl)- 3-(4-(trifluoromethyl) phenyl)quinoxalin-2-yl) pentanoic acid
577


 


425




5-(3-(4-chlorophenyl)-7-(3-(3- chlorophenyl)azetidine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
534


 


426




5-(3-(4-chlorophenyl)-7-(3-(4- chlorophenyl)azetidine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
534


 


427




5-(7-(((R)-1-(4- chlorophenyl)ethyl)carbamoyl)-3- (4-fluorophenyl)quinoxalin- 2-yl)-2-methylpentanoic acid
520


 


428




5-(7-(((S)-1-(4- chlorophenyl)ethyl)carbamoyl)-3- 3-(4-fluorophenyl)quinoxalin- 2-yl)-2-methylpentanoic acid
520


 


429




5-(3-(4-chlorophenyl)-7-(((R)- 1-(4-fluorophenyl)ethyl) carbamoyl)quinoxalin-2-yl)-3- methylpentanoic acid
520


 


430




5-(3-(4-fluorophenyl)-7-(((S)- 1-(4- fluorophenyl)ethyl)carbamoyl) quinoxalin-2-yl)-3- methylpentanoic acid
504


 


431




5-(3-(4-fluorophenyl)-7-(((R)- 1,2,3,4-tetrahydronaphthalen- 1-yl)carbamoyl)quinoxalin-2- yl)-3-methylpentanoic acid
512


 


432




5-(3-(4-fluorophenyl)-7-(((R)- 1-(4-fluorophenyl)ethyl) carbamoyl)quinoxalin-2-yl)-3- methylpentanoic acid
504


 


433




5-(3-(4-fluorophenyl)-7-(((S)- 1,2,3,4-tetrahydronaphthalen- 1-yl)carbamoyl)quinoxalin-2- yl)-3-methylpentanoic acid
512


 


434




(R)-5-(3-(4-chlorophenyl)-7- ((1-(5-fluoropyridin-2- yl)ethyl)carbamoyl)quinoxalin- 2-yl)pentanoic acid
507


 


435




(S)-5-(3-(4-chlorophenyl)-7- ((1-(5-fluoropyridin-2- yl)ethyl)carbamoyl)quinoxalin- 2-yl)pentanoic acid
507


 


436




5-(3-(4-chlorophenyl)-7-(3- (trifluoromethyl)-5,6,7,8- tetrahydro-[1,2,4]triazolo[4,3- a]pyrazine-7- carbonyl)quinoxalin-2- yl)pentanoic acid
559


 


437




(R)-5-(3-(4-fluorophenyl)-7- (((R)-1-(4-fluorophenyl)ethyl) carbamoyl)quinoxalin-2-yl)-3- methylpentanoic acid
504


 


438




(S)-5-(3-(4-fluorophenyl)-7- (((R)-1-(4- fluorophenyl)ethyl) carbamoyl)quinoxalin-2-yl)-3- methylpentanoic acid
504


 


439




(R)-5-(3-(4-fluorophenyl)-7- (((R)-1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)quinoxalin-2-yl)- 3-methylpentanoic acid
512


 


440




(S)-5-(3-(4-fluorophenyl)-7- (((R)-1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)quinoxalin-2-yl)- 3-methylpentanoic acid
512


 


441




(R)-5-(3-(4-chlorophenyl)-7- (((R)-1-(4- fluorophenyl)ethyl)carbamoyl) quinoxalin-2-yl)-3- methylpentanoic acid
520


 


442




(S)-5-(3-(4-chlorophenyl)-7- fluorophenyl)ethyl)carbamoyl) quinoxalin-2-yl)-3- methylpentanoic acid
520


 


443




5-(7-(benzylcarbamoyl)-3-(4- fluorophenyl)quinoxalin-2-yl)- 2,2-difluoropentanoic acid
494


 


444




2,2-difluoro-5-(7-(4-((4- methylbenzyl)carbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
490


 


445




2-(3-(4-chlorophenyl)-7- (4-phenylpiperazine-1- carbonyl)quinoxalin-2- yl)ethyl)-5-fluorophenyl) acetic acid
610


 


446




2-(3-(2-(7-((4- chlorobenzypcarbamoyl)-3-(4- chlorophenyl)quinoxalin-2- yl)ethyl)-5-fluorophenyl) acetic acid
589


 


447




2-(3-(2-(3-(4-chlorophenyl)-7- ((4-fluorobenzyl)(methyl) carbamoyl)quinoxalin-2- yl)ethyl)-5- fluorophenyl)acetic acid
586


 


448




2-(3-(2-(7- (benzyl(methyl)carbamoyl)-3- (4-chlorophenyl)quinoxalin-2- yl)ethyl)-5-fluorophenyl) acetic acid
568


 


449




(R)-2-(3-2-(3-(4- chlorophenyl)-7-(1-(4- fluorophenyl)ethyl)carbamoyl) quinoxalin-2-yl)ethyl)-5- fluorophenyl)acetic acid
586


 


450




(S)-2-(3-(2-(3-(4- chlorophenyl)-7-((1-(4- fluorophenyl)ethyl)carbamoyl) quinoxalin-2-yl)ethyl)-5- fluorophenyl)acetic acid
586


 


451




2-(3-(2-(3-(4-chlorophenyl)-7- ((4-cyanobenzyl)carbamoyl) quinoxalin-2-yl)ethyl)-5- fluorophenyl)acetio acid
579


 


452




2-(3-(2-(3-(4-chlorophenyl)-7- (3-(3,4- difluorophenyl)azetidine-l- carbonyl)quinoxalin-2- yl)ethyl)-5-fluorophenyl) aceticacid
616


 


453




2-methyl-5-(3-phenyl-7-(((R)- 1-yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
494


 


454




5-(3-(4-fluorophenyl)-7-(((R)- 1,2,3 ,4-tetrahydronaphthalen- 1-yl)carbamoyl)quinoxalin-2- yl)-2-methy)pentanoic acid
512


 


455




5-(7-(((R)-6-chloro-1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)-3-(4- fluorophenyl)quinoxalin-2-yl)- 2-methylpentanoic acid
546


 


456




5-(7-(((R)-5-chloro-2,3- dihydro-1H-inden-1- yl)carbamoyl)-3-(4- fluorophenyl)quinoxalin-2-yl)- 2-methylpentanoic acid
532


 


457




5-(7-(((R)-5-fluoro-2,3- dihydro-1H-inden-1- yl)carbamoyl)-3-(4- fluorophenyl)quinoxalin-2-yl)- 2-methylpentanoic acid
516


 


458




5-(7-(((R)-6-chloro-1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)-3- phenylquinoxalin-2-yl)-2- methylpentanoic acid
528


 


459




5-(7-(((R)-6-fluoro-1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)-3- phenylquinoxalin-2-yl)-2- methylpentanoic acid
512


 


460




5-(7-(((R)-1-(4- chlorophenyl)ethyl) carbamoyl)-3- 3-phenylquinoxalin- 2-yl)-2- methylpentanoic acid
502


 


461




5-(7-(((R)-1-(4- fluorophenyl)ethyl) carbamoyl)-3- phenylquinoxalin-2-yl)-2- methylpentanoic acid
486


 


462




2-methyl-5-(3-phenyl-7-(4- phenylpiperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
509


 


463




2-methyl-5-(3-phenyl-7- (((1R,2S)-2- phenylcyclopropyl) carbamoyl) quinoxalin-2-yl) pentanoic acid
480


 


464




(R)-5-(7-((1-(4- chlorophenyl)ethyl) carbamoyl)-3- phenylquinoxalin- 2-yl)-2,2- difluoropentanoic acid
524


 


465




(R)-2,2-difluoro-5-(7-((6- fluoro-1,2,3,4- tetrabydronaphthalen-1- yl)carbamoyl)-3-(4- fluorophenyl)quinoxalin-2- yl)pentanoic acid
552


 


466




(R)-2,2-difluoro-5- (3-phenyl- 7-((1,2,3,4- tetahydronaphthalen-1- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
516


 


467




(R)-5-(7-((6-chloro-1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)-3- phenylquinoxalin-2-yl)-2,2- difluoropentanoic acid
550


 


468




(R)-2,2-difluoro-5-(7-((5- fluoro-2,3-dihydro-1H-inden- 1-yl)carbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
520


 


469




(R)-2,2-difluoro-5-(3-phenyl- 7-((2,2,2-trifluoro-1-(4- fluorophenyl)ethyl)carbamoyl) quinoxalin-2-yl)pentanoic acid
562


 


470




2,2-difluoro-5-(3- phenyl-7-(4- phenylpiperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
531


 


471




(R)-5-(3-(4-fluorophenyl)-7- ((1-(5-fluoropyridin-2- yl)ethyl)carbamoyl) quinoxalin- 2-yl)pentanoic acid
491


 


472




(S)-5-(3-(4-fluorophenyl)-7- ((1-(5-fluoropyridin-2- yl)ethyl)carbamoyl) quinoxalin- 2-yl)pentanoic acid
491


 


473




(R)-5-(7-((1-(5-fluoropyridin- 2-yl)ethyl)carbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
473


 


474




(S)-5-(7-((1-(5-fluoropyridin- 2-yl)ethyl)carbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
473


 


475




(R)-5-(7-((6-chloro-1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)-3-(4- fluorophenyl)quinoxalin-2-yl)- 2,2-difluoropentanoic acid
568


 


476




(R)-2,2-dilluoro-5-(7-((5- fluoro-2,3-dihydro-1H-inden- 1-yl)carbamoyl)-3-(4- fluorophenyl)quinoxalin-2- yl)pentanoic acid
538


 


477




(R)-5-(3-(4-fluorophenyl)-7- (((R)-1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)quinoxalin-2-yl)- 2-methylpentanoic acid
512


 


478




(R)-5-(3-(4-fluorophenyl)-7- (((S)-1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)quinoxalin-2-yl)- 2-methylpentanoic acid
512


 


479




(R)-5-(3-(4-fluorophenyl)-7- fluorophenyl)ethyl) carbamoyl) quinoxalin-2-yl)-2- methylpentanoic acid
504


 


480




(R)-2,2-difluoro-5-(7-((6- fluoro-1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
534


 


481




(R)-5-(3-(4-fluorophenyl)-7- (((S)-1-(4- fluorophenyl)ethyl) carbamoyl) quinoxalin-2-yl)-2- methylpentanoic acid
504


 


482




(S)-5-(3-(4-fluorophenyl)-7- (((R)-1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)quinoxalin-2-yl)- 2-methylpentanoic acid
512


 


483




(S)-5-(3-(4-fluorophenyl)-7- (((S)-1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl) quinoxalin-2-yl)- 2-methylpentanoic acid
512


 


484




(S)-5-(3-(4-fluorophenyl)-7- (((R)-1-(4- fluorophenyl)ethyl) carbamoyl) quinoxalin-2-yl)-2- methylpentanoic acid
504


 


485




(S)-5-(3-(4-fluorophenyl)-7- (((S)-1-(4- fluorophenyl)ethyl) carbamoyl) quinoxalin-2-yl)-2- methylpentanoic acid
504


 


486




(R)-2,2-difluoro-5-(3-(4- fluorophenyl)-7-((1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl) quinoxalin-2- yl)pentanoic acid
534


 


487




(R)-2,2-difluoro-5-(3-(4- fluorophenyl)-7-((1-(4- fluorophenyl)ethyl) carbamoyl) quinoxalin-2-yl) pentanoic acid
526


 


488




(R)-5-(3-(4-chlorophenyl)-7- (4-(4-fluorophenyl)-2- methylpiperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
561


 


489




(R)-5-(3-(4-fluorophenyl)-7- (4-(4-fluorophenyl)-2- methylpiperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
545


 


490




(S)-5-(3-(4-chlorophenyl)-7- (4-(4-fluorophenyl)-2- methylpiperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
561


 


491




(R)-5-(7-(chroman-4- ylcarbamoyl)-3-(4- fluorophenyl)quinoxalin-2- yl)pentanoic acid
500


 


492




(S)-5-(7-(chroman-4- ylcarbamoyl)-3-(4- fluorophenyl)quinoxalin-2- yl)pentanoic acid
500


 


493




(R)-5-(3-(4-chlorophenyl)-7- ((1-(naphthalen-1- yl)ethyl)carbamoyl) quinoxalin- 2-yl)pentanoic acid
539


 


494




(S)-5-(3-(4-chlorophenyl)-7- ((1-(naphthalen-1- yl)ethyl)carbamoyl) quinoxalin- 2-yl)pentanoic acid
539


 


495




(R)-5-(3-(4-chlorophenyl)-7- ((1-(naphthalen-2- yl)ethyl)carbamoyl) quinoxalin- 2-yl)pentanoic acid
539


 


496




(S)-5-(3-(4-chlorophenyl)-7- ((1-(naphthalen-2- yl)ethyl)carbamoyl) quinoxalin- 2-yl)pentanoic acid
539


 


497




(S)-5-(3 -(4-fluoro- phenyl)-7-(4- (4-fluorophenyl)-2- methylpiperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
545


 


498




(R)-5-(3-(4-chlorophenyl)-7- (2-methyl-4-phenyl- piperazine- 1-carbonyl)quinoxalin-2- yl)pentanoic acid
543


 


499




(R)-5-(3-(4-fluorophenyl)-7- (2-methyl-4-phenyl- piperazine- 1-carbonyl)quinoxalin-2- yl)pentanoic acid
527


 


500




(1R,2R)-2-(2-(3-(4- fluorophenyl)-7- (((R)-1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)quinoxalin-2- yl)ethyl)cyclopropane- carboxylic acid
510


 


501




2-(2-(3-(4-fluorophenyl)-7- (((R)-1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)quinoxalin-2- yl)ethyl)cyclopropane- carboxylic acid
510


 


502




5-(3-phenyl-7-((3- (trifluoromethyl)-5,6,7,8- tetrahydroquinolin-8- yl)carbamoyl) quinoxalin-2- yl)pentanoic acid
549


 


503




(R)-5-(3-phenyl-7-((5,6,7,8- tetrahydroquinolin-5- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
481


 


504




(S)-5-(3-phenyl-7-((5,6,7,8- tetrahydroquinolin-5- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
481


 


505




5-(3-phenyl-7-((5,6,7,8- tetrahydroisoquinolin-5- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
481


 


506




(R)-5-(3-(4-chlorophenyl)-7- (chroman-4- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
516


 


507




(S)-5-(3-(4-chlorophenyl)-7- (chroman-4- ylcarbamoyl)quinoxalin-2- yl)pentanoic acid
516.3


 


508




5-(3-(4-fluorophenyl)-7- (((1S,2R)-2-hydroxy-2,3- dihydro-1H-inden-1- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
500


 


509




5-(3-(4-fluorophenyl)-7- (((1R,2S)-2-hydroxy-2,3- dihydro-1H-inden-1- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
500


 


510




5-(3-(4-fluorophenyl)-7- (((1R,2R)-2-hydroxy-2,3- dihydro-1H-inden-1- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
500


 


511




5-(3-(4-fluorophenyl)-7- (((1S,2S)-2-hydroxy-2,3- dihydro-1H-inden-1- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
500


 


512




(R)-5-(7-(chroman-4- ylcarbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
482


 


513




(S)-5-(7-(chroman-4- ylcarbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
482


 


514




(S)-5-(3-(4-chlorophenyl)-7- (2-methyl-4-phenyl- piperazine- 1-carbonyl)quinoxalin-2- yl)pentanoic acid
543


 


515




(S)-5-(3-(4-fluorophenyl)-7-(2- methyl-4-phenylpiperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
527


 


516




(S)-5-(7-(4-(4-fluorophenyl)-2- methylpiperazine-1-carbonyl)- 3-phenylquinoxalin-2- yl)pentanoic acid
527


 


517




(S)-5-(7-(((R)-1-(4- chlorophenyl)ethyl) carbamoyl)- 3-(4-fluorophenyl) quinoxalin- 2-yl)-2-methyl- pentanoic acid
520


 


518




(S)-5-(7-(((R)-6-fluoro- 1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)-3-(4- fluorophenyl)quinoxalin- 2-yl)- 2-methylpentanoic acid
530


 


519




(S)-5-(7-(((R)-6-chloro- 1,2,3,4-tetrahydronaphthalen- 1-yl)carbamoyl)-3-(4- fluorophenyl) quinoxalin-2-yl)- 2-methylpentanoic acid
546


 


520




(S)-5-(7-((R)-chroman-4- ylcarbamoyl)-3-(4- fluorophenyl)quinoxalin-2-yl)- 2-methylpentanoic acid
514


 


521




(S)-5-(3-(4-fluorophenyl)-7- (((R)-5,6,7,8- tetrahydroquinolin-5- yl)carbamoyl)quinoxalin-2-yl)- 2-methylpentanoic acid
513


 


522




(S)-5-(7-(((R)-5-chloro-2,3- dihydro-1H-inden-1- yl)carbamoyl)-3-(4- fluorophenyl)quinoxalin-2-yl)- 2-methylpentanoic acid
532


 


523




(S)-5-(7-(((R)-5-fluoro-2,3- dihydro-1H-inden-1- yl)carbamoyl)-3-(4- fluorophenyl)quinoxalin-2-yl)- 2-methylpentanoic acid
516


 


524




(S)-5-(3-(4-fluorophenyl)-7-(4- phenylpiperazine-1- carbonyl)quinoxalin-2-yl)-2- methylpentanoic acid
527


 


525




5-(3-(4-fluorophenyl)-7-(((5- methyl-1,3,4-oxadiazol-2- yl)methyl)carbamoyl) quinoxalin-2-yl) pentanoic acid
464


 


526




(R)-5-(6-methyl-3-phenyl-7- ((1,2,3,4-tetrahydronaphthalen- 1-yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
494


 


527




(R)-5-(7-((1-(4- chlorophenyl)ethyl)carbamoyl)- 3-(4-fluorophenyl)quinoxalin- 2-yl)-2,2-difluoropentanoic acid
542


 


528




(R)-2,2-difluoro-5-(3-(4- fluorophenyl)-7-((2,2,2- trifluoro-1-(4-fluorophenyl) ethyl)carbamoyl) quinoxalin-2-yl)pentanoic acid
580


 


529




2,2-difluoro-5-(7-((4- fluorobenzyl)(methyl) carbamoyl)- 3-(4-fluorophenyl) quinoxalin-2- yl)pentanoic acid
526


 


530




2,2-difluoro-5-(3-(4- fluorophenyl)-7-(4- phenylpiperazine-1- yl)pentanoic acid
549


 


531




2,2-difluoro-5-(3-(4- fluorophenyl)-7-(4- phenylpiperidine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
548


 


532




(R)-5-(3-(4-chlorophenyl)-7- ((7-fluorochroman-4- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
534


 


533




(R)-5-(3-(4-chlorophenyl)-7- ((6,7-difluorochroman-4- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
552


 


534




(S)-5-(3-(4-chlorophenyl)-7- ((6-fluorochroman-4- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
534


 


535




(S)-5-(3-(4-fluorophenyl)-7- ((1-(naphthalen-2- yl)ethyl)carbamoyl)quinoxalin- 2-yl)pentanoic acid
522


 


536




(S)-5-(3-(4-fluorophenyl)-7- ((1-(naphthalen-1- yl)ethyl)carbamoyl)quinoxalin- 2-yl)pentanoic acid
522


 


537




(R)-5-(7((6-fluorochroman-4- yl)carbamoyl)-3-(4- fluorophenyl)quinoxalin-2- yl)pentanoic acid
518


 


538




(R)-5-(7-((6,7- difluorochroman-4- yl)carbamoyl)-3-(4- fluorophenyl)quinoxalin-2- yl)pentanoic acid
536


 


539




(R)-5-(3-(4-chlorophenyl)-7- (4-(4-chlorophenyl)-2- methylpiperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
577


 


540




(R)-5-(7-(4-(4-chlorophenyl)- 2-methylpiperazine-1- carbonyl)-3-(4- fluorophenyl)quinoxalin-2- yl)pentanoic acid
561


 


541




(S)-5-(3-(4-chlorophenyl)-7- (4-(4-chlorophenyl)-2- methylpiperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
577


 


542




(S)-5-(7-(4-(4-chlorophenyl)- 2-methylpiperazine-1- carbonyl)-3-(4- fluorophenyl)quinoxalin-2- yl)pentanoic acid
561


 


543




2-(3-(3-(3-(4-chlorophenyl)-7- (4-phenylpiperazine-1- carbonyl)quinoxalin-2- yl)propyl)phenyl)acetic acid
601


 


544




2-(3-(3-(3-(4-chlorophenyl)-7- ((4-fluorobenzyl) (methyl)carbamoyl) quinoxalin-2- yl)propyl)phenyl)acetic acid
582


 


545




(R)-2-(3-(3-(3-(4- chlorophenyl)-7-((1-(4- fluorophenyl)ethyl) carbamoyl) quinoxalin-2- yl)propyl)phenyl)acetic acid
582


 


546




2-(3-(3-(7-((4- chlorobenzyl) carbamoyl)-3-(4- chlorophenyl) quinoxalin-2- yl)propyl)phenyl) acetic acid
586


 


547




(R)-5-(7-chroman-4- ylcarbamoyl)-3-(4- fluorophenyl) quinoxalin-2-yl)- 2,2-difluoro- pentanoic acid
536


 


548




(S)-5-(7-(chroman-4- ylcarbamoyl)-3-(4- fluorophenyl) quinoxalin-2-yl)- 2,2-difluoro- pentanoic acid
536


 


549




(S)-5-(7-(2-methyl-4- phenylpiperazine-l- carbonyl)- 3-phenylquinoxalin-2- yl)pentanoic acid
509


 


550




(R)-5-(7-(4-(4-chlorophenyl)- 2-methylpiperazine-1- carbonyl)-3-phenyl- quinoxa1in- 2-yl)pentanoic acid
543


 


551




(S)-5-(7-(4-(4-chlorophenyl)- 2-methylpiperazine-1- carbonyl)-3-phenyl- quinoxalin- 2-yl)pentanoic acid
543


 


552




5-(3-(4-chlorophenyl)-7-(4- (pyrimidin-2-yl)piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
531.3


 


553




5-(3-(4-chlorophenyl)-7-(3- oxo-4-phenylpiperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
543.3


 


554




5-(3-(4-chlorophenyl)-7-(4- (pyridin-2-yl)piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
530.3


 


555




5-(7-(4-benzylpiperazine-1- carbonyl)-3-(4- chlorophenyl)quinoxalin-2- yl)pentanoic acid
543.3


 


556




5-(3-(4-chlorophenyl)-7-(4,4- dimethyl-1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
542.3


 


557




5-(3-(4-chlorophenyl)-7-(6- fluoro-1,2,3,4- tetrahydroisoquinoline-2- carbonyl)quinoxalin-2- yl)pentanoic acid
518.2


 


558




5-(3-(4-chlorophenyl)-7-(5- fluoro-1,1- dimethylisoindoline-2- carbonyl)quinoxalin-2- yl)pentanoic acid
532.3


 


559




(R)-5-(3-(4-chlorophenyl)-7- (3-(4-fluorophenyl) pyrrolidine- 1-carbonyl)quinoxalin-2- yl)pentanoic acid
532.3


 


560




(S)-5-(3-(4-chlorophenyl)-7- (3-(4-fluorophenyl) pyrrolidine- 1-carbonyl)quinoxalin-2- yl)pentanoic acid
532.3


 


561




5-(3-(4-chlorophenyl)-7- ((2,3,4,5- tetrahydrobenzo[b]oxepin-5- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
530.3


 


562




(R)-5-(3-(4-chlorophenyl)-7- ((2,3-dihydrobenzofuran-3- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
502.2


 


563




(S)-5-(3-(4-chlorophenyl)-7- ((2,3-dihydrobenzofuran-3- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
502.2


 


564




5-(3-(4-chlorophenyl)-7-((2,2- dimethylchroman-4- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
544.3


 


565




5-(3-(4-chlorophenyl)-7-(3,4- dihydro-2H-spiro [naphthalene- 1,3′-pyrrolidin]-1′- ylcarbonyl)quinoxalin-2- yl)pentanoic acid
554.3


 


566




5-(3-(4-chlorophenyl)-7-((2- oxoindolin-3- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
515.2


 


567




5-(3-(4-chlorophenyl)-7-(3- oxo-3,4-dihydro-2H- spiro[isoquinoline-1,4′- piperidin+9-1′- ylcarbonyl)quinoxalin-2- yl)pentanoic acid
583.3


 


568




5 -(3-(4-chlorophenyl)-7-( 1- oxo-2,4-dihydro-1H- spiro[isoquinoline-3,4′- piperidin]-1′- ylcarbonyl)quinoxalin-2- yl)pentanoic acid
583.3


 


569




(R)-5-(3-(4-chlorophenyl)-7- (thiochroman-4- ylcarbamoyl)quinoxalin-2- yl)pentanoic acid
532.3


 


570




(R)-5-(3-(4-chlorophenyl)-7- ((6,7,8,9-tetrahydro-5H- benzo[7]annulen-5- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
528.3


 


571




(S)-5-(7-((1-(4- fluoronaphthalen-1- yl)ethyl)carbamoyl)-3-(4- fluorophenyl)quinoxalin-2- yl)pentanoic acid
540


 


572




(R)-5-(7-((1-(4- fluoronaphthalen-1- yl)ethyl)carbamoyl)-3-(4- fluorophenyl)quinoxalin-2- yl)pentanoic acid
540


 


573




(S)-5-(7-((2,3- dihydrobenzofuran-3- yl)carbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
468


 


574




(R)-5-(7-((2,3- dihydrobenzofuran-3- yl)carbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
468


 


575




5-(7-((1-(methylsulfonyl)- 1,2,3,4-tetrahydroquinolin-4- yl)carbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
559


 


576




5-(7-((2-oxo-1,2,3,4- tetrahydroquinolin-4- yl)carbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
495


 


577




(R)-5-(3-(4-chlorophenyl)-7- (3-(4-fluorophenyl) pyrrolidine- 1-carbonyl)quinoxalin-2- yl)pentanoic acid
532.3


 


578




(S)-5-(3-(4-chlorophenyl)-7- (3-(4-fluorophenyl) pyrrolidine- 1-carbonyl)quinoxalin-2- yl)pentanoic acid
532.3


 


579




5-(3-(4-chlorophenyl)-7- ((2,3,4,5- tetralaydrobenzo[b]oxepin-5- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
530.3


 


580




(R)-5-(3-(4-chlorophenyl)-7- ((2,3-dihydrobenzofuran-3- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
502.2


 


581




(S)-5-(3-(4-chlorophenyl)-7- ((2,3-dihydrobenzofuran-3- yl)carbatnoyl)quinoxalin-2- yl)pentanoic acid
502.2


 


582




5-(3-(4-chlorophenyl)-7-((2,2- dimethylchroman-4- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
544.3


 


583




5-(3-(4-chlorophenyl)-7-(3,4- dihydro-2H-spiro [naphthalene- 1,3′-pyrrolidin]-1′- ylcarbonyl)quinoxalin-2- yl)pentanoic acid
554.3


 


584




5-(3-(4-chlorophenyl)-7-((2- oxoindolin-3- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
515.2


 


585




5-(3-(4-chlorophenyl)-7-(3- oxo-3,4-dihydro-2H- spiro[isoquinoline-1,4′- piperidin]-1′- ylcarbonyl)quinoxalin-2- yl)pentanoic acid
583.3


 


586




5-(3-(4-chlorophenyl)-7-(1- oxo-2,4-dihydro-1H- spiro[isoquinotine-3,4′- piperidin]-1′- ylcarbonyl)quinoxalin-2- yl)pentanoic acid
583.3


 


587




(R)-5-(3-(4-chlorophenyl)-7- (thiochroman-4- ylcarbamoyl)quinoxalin-2- yl)pentanoic acid
532.3


 


588




(R)-5-(3-(4-chlorophenyl)-7- ((6,7,8,9-tetrahydro-5H- benzo[7]annulen-5- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid.
528.3


 


589




(R)-2,2-difluoro-5-(3-(4- fluorophenyl)-7-((6- methoxy- 1,2,3,4-tetrahydro- naphthalen- 1-yl)carbamoyl) quinoxalin-2- yl)pentanoic acid
564


 


590




5-(7-(4- ((benzyloxy)carbonyl) piperazine-1- carbonyl)-3-(4- chlorophenyl) quinoxalin-2- yl)pentanoic acid
587.4


 


591




5-(7-(4-benzyl-1,4- diazepane- 1-carbonyl)-3-(4- chlorophenyl) quinoxalin-2- yl)pentanoic acid
557.3


 


592




5-(3-(4-chlorophenyl)-7-(4- (furan-2-carbonyl) piperazine- 1-carbonyl) quinoxalin-2- yl)pentanoic acid
547.3


 


593




5-(7-(benzyl(methyl) carbamoyl)-3- (4-chlorophenyl) quinoxalin-2- yl)pentanoic acid
488.2


 


594




5-(3-(4-chlorophenyl)-7-(4- (pyridin-4-yl)piperazine-l- carbonyl)quinoxalin-2- yl)pentanoic acid
530.3


 


595




5-(3-(4-chlorophenyl)- 7-(4-(3- cyanopyridin-2-yl) piperazine- 1-carbonyl) quinoxalin-2- yl)pentanoic acid
555.3


 


596




5-(3-(4-chlorophenyl)-7-(4- (3,5-dichloropyridin-4- yl)piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
600.2


 


597




5-(3-(4-chlorophenyl)-7-(4- (pyrazin-2-yl)piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
531.3


 


598




5-(3-(4-chlorophenyl)-7- (octahydropyrrolo[1,2- a]pyrazine-2- carbonyl)quinoxalin-2- yl)pentanoic acid
493.3


 


599




5-(3-(4-chlorophenyl)- 7-(4-(5- yl)piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
599.3


 


600




5-(3-(4-chlorophenyl)-7-(4-(2- (trifluoromethyl)phenyl) piperazine-1-carbonyl) quinoxalin-2- yl)pentanoic acid
597.3


 


601




5-(3-(4-chlorophenyl)-7-(4- (thiazol-2-yl)piperazine-1- carbonyl) quinoxalin-2- yl)pentanoic acid
536.2


 


602




5-(7-(4-(benzo[d]thiazol-2- yl)piperazine-1- carbonyl)-3-(4- chlorophenyl)quinoxalin-2- yl)pentanoic acid
586.3


 


603




5-(3-(4-chlorophenyl)-7- (1,2,3,4- tetrahydroisoquinoline-2- carbonyl)quinoxalin-2- yl)pentanoic acid
500.3


 


604




5-(3-(4--chlorophenyl)-7-(3- cyano-3-phenyl- pyrrolidine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
539.3


 


605




5-(3-(4-chlorophenyl)-7-((1- (methylsulfonyl)-1,2,3,4- tetrahydroquinolin-4- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
539.3


 


606




5-(3-(4-chlorophenyl)-7-(3-(4- fluorophenyl)azetidine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
518.3


 


607




5-(3-(4-chlorophenyl)-7- (isoindoline-2- carbonyl)quinoxalin-2- yl)pentanoic acid
486.3


 


608




5-(3-(4-chlorophenyl)-7- (2,3,4,9-tetrahydro-1H- pyrido[3,4-b]indole-2- carbonyl)quinoxalin-2- yl)pentanoic acid
539.3


 


609




5-(3-(4-chlorophenyl)-7-(4-(2- chlorophenyl)piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
563.3


 


610




5-(3-(4-chlorophenyl)-7-(4-(3- (trifluoromethyl)phenyl) piperazine-1-carbonyl) quinoxalin-2- yl)pentanoic acid
597.7


 


611




5-(7-(butyl(propyl)carbamoyl)- 3-(4-chlorophenyl) quinoxalin- 2-yl)pentanoic acid
492.3


 


612




(S)-5-(3-(4-chlorophenyl)-7- ((7-fluorochroman-4- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
534.3


 


613




(S)-5-(3-(4-chlorophenyl)-7- (2-(4-fluorophenyl) morpholine-4- carbonyl)quinoxalin-2- yl)pentanoic acid
548


 


614




(R)-5-(3-(4-chlorophenyl)-7- (2-(4-fluorophenyl) morpholine-4- carbonyl)quinoxalin-2- yl)pentanoic acid
548


 


615




(S)-5-(3-(4-chlorophenyl)-7- (2-(4-(trifluoromethyl)phenyl) morpholine-4-carbonyl) quinoxalin-2- yl)pentanoic acid
598


 


616




(R)-5-(3-(4-chlorophenyl)-7- (2-(4-(trifluoromethyl)phenyl) morpholine-4-carbonyl) quinoxalin-2- yl)pentanoic acid
598


 


617




(R)-5-(3-(4-chlorophenyl)-7- (5,6,7,8- tetrahydroisoquinolin-8- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
515.4


 


618




(S)-5-(3-(4-chlorophenyl)-7- ((5,6,7,8- tetrahydroisoquinolin-8- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
515.4


 


619




5-(3-(4-chlorophenyl)- 7-(4-(o- tolyl)piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
543.3


 


620




5-(3-(4-chlorophenyl)- 7-(4-(m- tolyl)piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
543.3


 


621




5-(3-(4-chlorophenyl)- 7-(4- (3,4-dimethylphenyl) piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
557.4


 


622




5-(3-(4-chlorophenyl)- 7-(4-(4- methoxyphenyl) piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
559.4


 


623




5-(3-(4-chlorophenyl)- 7-(4-(4- cyanophenyl)piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
554.4


 


624




5-(3-(4-chlorophenyl)-7-(4- (3,4-dichlorophenyl) piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
597.3


 


625




5-(3-(4-chlorophenyl)-7- (methyl(pyridin-3- ylmethyl)carbamoyl) quinoxalin-2- yl)pentanoic acid
489.3


 


626




5-(3-(4-chlorophenyl)- 7-(4-(2- oxo-2,3-dihydro-1H- benzo[d]imidazo l-1- yl)piperidine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
584.4


 


627




5-(3-(4-chlorophenyl)- 7-(4- (2,3-dimethylphenyl) piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
557.4


 


628




5-(3-(4-chlorophenyl)- 7-(4- (2,6-dimethylphenyl) piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
557.4


 


629




5-(3-(4-chlorophenyl)- 7-(4- (2,5- dimethylphenyl) piperazine-l- carbonyl)quinoxalin-2- yl)pentanoic acid
557.4


 


630




5-(7-(1S,4S)-5-benzyl-2,5- diazabicyclo[2.2.1] heptane-2- carbonyl)-3-(4- chlorophenyl)quinoxalin-2- yl)pentanoic acid
555.4


 


631




5-(3-(4-chlorophenyl)- 7-(4-(2- cyanophenyl) piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
554.4


 


632




5-(3-(4-chlorophenyl)- 7-(4-(2- methoxyphenyl) piperidine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
558.4


 


633




5-(3-(4-chlorophenyl)- 7-(3H- spiro[isobenzofuran-1,4′- piperidin]-1′- ylcarbonyl)quinoxalin-2- yl)pentanoic acid
556.4


 


634




5-(3-(4-chlorophenyl)-7-(1- oxo-2-phenyl-2,8- diazaspiro[4.5]decane-8- carbonyl)quinoxalin-2- yl)pentanoic acid
597.5


 


635




5-(3-(4-chlorophenyl)-7-(4- (2,3-dichlorophenyl) piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
597.3


 


636




5-(3-(4-chlorophenyl)-7-(4- (2,4-dimethylphenyl) piperazine-1- carbonyl)quinoxa1in-2- yl)pentanoic acid
557.4


 


637




(S)-5-(7-((1-(isoquinolin-4- yl)ethyl)carbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
505


 


638




(R)-5-(7-((1-(isoquinolin-4- yl)ethyl)carbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
505


 


639




(R)-5-(3-phenyl-7-((1- (quinolin-5- yl)ethyl)carbamoyl) quinoxalin- 2-yl)pentanoic acid
505


 


640




(S)-5-(3-phenyl-7-((1- (quinolin-5- yl)ethyl)carbamoyl) quinoxalin- 2-yl)pentanoic acid
505


 


641




(R)-5-(7-((6-fluoro- chroman-4- yl)carbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
500


 


642




(R)-5-(7-((6,7- difluorochroman-4- yl)carbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
518


 


643




(R)-5-(7-((7-fluoro- chroman-4- yl)carbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
500


 


644




(S)-5-(3-(4-chloro- phenyl)-7- (methyl(1- phenylethyl)carbamoyl) quinoxalin-2-yl) pentanoic acid
502.3


 


645




5-(3-(4-chlorophenyl)-7-(2- phenylpiperidine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
528.3


 


646




5-(3-(4-chlorophenyl)-7-(2- phenylpyrrolidine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
514.3


 


647




5-(3-(4-chlorophenyl)-7-(2- (pyridin-2-yl)piperidine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
529.3


 


648




5-(3-(4-chlorophenyl)-7-(2- phenylazetidine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
500.3


 


649




5-(3-(4-chlorophenyl)- 7-((1-(furan-3- yl)ethyl)(methyl) carbamoyl) quinoxalin-2-yl) pentanoic acid
492.3


 


650




5-(3-(4-chlorophenyl)-7-(2- (pyridin-4-yl)pyrrolidine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
515.3


 


651




5-(3-(4-chlorophenyl)- 7-(2-(1- methyl-1H-imidazol-2- yl)piperidine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
532.3


 


652




5-(3-(4-chlorophenyl)- 7-(2-(4- methyl-1,2,5- oxad iazol-3- yl)pyrrolidine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
520.3


 


653




5-(3-(4-chlorophenyl)-7-(2- (pyridin-3-yl)azepane-1- carbonyl)quinoxalin-2- yl)pentanoic acid
543.3


 


654




5-(3-(4-chlorophenyl)-7-(2- (thiazol-2-yl)piperidine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
535.3


 


655




5-(3-(4-chlorophenyl)-7- (methyl(1-(4- methylthiazol-2- yl)ethyl)carbamoyl) quinoxalin- 2-yl)pentanoic acid
523.3


 


656




5-(3-(4-chlorophenyl)-7-(5- fluoro-2,3- dihydrospiro [indene- 1,2′- pyrrolidin]-1′- ylcarbonyl)quinoxalin-2- yl)pentanoic acid
558.3


 


657




5-(3-(4-chlorophenyl)-7-((1- (isoxazol-3- yl)ethyl)(methyl)carbamoyl) quinoxalin-2-yl) pentanoic acid
493.3


 


658




5-(3-(4-chloro- phenyl)-7-(5,6- dihydrospiro [cyclopenta[c] pyridine-7,2′- pyrrolidin]-1′- ylcarbonyl) quinoxalin-2- yl)pentanoic acid
541.3


 


659




5-(3-(4-chlorophenyl)-7-(2- (thiazol-2-yl)pyrrolidine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
521.2


 


660




5-(3-(4-chlorophenyl)- 7-(2-(3- methyl-1,2,4-oxadiazol-5- yl)piperidine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
534.3


 


661




(R)-5-(3-(4-chlorophenyl)-7- (3-phenylmorphohne-4- carbonyl)quinoxalin-2- yl)pentanoic acid
530.3


 


662




5-(3-(4-chlorophenyl)-7-(2- (pyridin-2-yl)pyrrolidine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
515.3


 


663




5-(3-(4-chlorophenyl)-7-(2- methyl-2,3,4,5-tetrahydro-1H- benzo[d]azepine-3- carbonyl)quinoxalin-2- yl)pentanoic acid
528.3


 


664




5-(3-(4-chlorophenyl)-7-(4-(4- methylbenzyl)piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
557.4


 


665




5-(3-(4-chlorophenyl)-7-(4-(p- tolyl)piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
543.3


 


666




5-(3-(4-chlorophenyl)-7-(4- methyl-2-phenylpiperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
543.3


 


667




5-(3-(4-chlorophenyl)-7-(4-(3- fluorobenzyl)piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
561.3


 


668




5-(3-(4-chlorophertyl)-7-(2- (trifluoromethyl)-5,6,7,8- tetrahydroimidazo[1,2- a]pyrazine-7- carbonyl)quinoxalin-2- yl)pentanoic acid
558.3


 


669




5-(3-(4-chlorophenyl)-7-(4-(5- chloropyridin-2-yl)piperazine- 1-carbonyl)quinoxalin-2- yl)pentanoic acid
564.3


 


670




5-(3-(4-chlorophenyl)-7-(4-((2- methylthiazol-4- yl)methyl)piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
564.3


 


671




5-(3-(4-chlorophenyl)-7-(4-(1- methyl-1H-pyrazol-4- yl)piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
533.3


 


672




5-(3-(4-chlorophenyl)-7- ((2S,5R)-2,5-dimethyl-4- phenylpiperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
557.4


 


673




5-(3-(4-chlorophenyl)-7-(3- ethyl-5,6,7,8-tetrahydro- [1,2,4]triazolo[4,3-a]pyrazine- 7-carbonyl)quinoxalin-2- yl)pentanoic acid
519.3


 


674




5-(3-(4-chlorophenyl)-7-(4- methyl-3-phenylpiperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
543.3


 


675




5-(3-(4-chlorophenyl)-7-(4-(4- methyl-1,2,5-oxadiazol-3- yl)piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
535.3


 


676




5-(3-(4-chlorophenyl)-7-(4-(3- chloropyridin-2-yl)piperazine- 1-carbonyl)quinoxalin-2- yl)pentanoic acid
564.3


 


677




5-(3-(4-chlorophenyl)-7- ((1S,4S)-5-(3-fluorophenyl)- 2,5-diazabicyclo[2.2.1]heptane-2- carbonyl)quinoxalin-2- yl)pentanoic acid
559.3


 


678




5-(3-(4-chlorophenyl)-7-(4- cyclohexyl-3-oxo- piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
549.4


 


679




5-(3-(4-chlorophenyl)-7-(4- (pyridin-3-y)piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
530.3


 


680




5-(3-(4-chlorophenyl)-7- ((1S,4S)-5-(4-fluorophenyl)- 2,5-diazabicyclo[2.2.1]heptane-2- carbonyl)quinoxalin-2- yl)pentanoic acid
559.3


 


681




5-(3-(4-chlorophenyl)-7-(4- (3,6-dimethylpyrazin-2- yl)piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
559.4


 


682




5-(3-(4-chlorophenyl)-7-(4- (pyridin-4- ylmethyl)piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
544.3


 


683




5-(3-(4-chlorophenyl)-7-(4-(3- methylpyridin-4-yl)piperazine- 1-carbonyl)quinoxalin-2- yl)pentanoic acid
544.3


 


684




5-(3-(4-chlorophenyl)-7-(4-(3- methylisothiazol-5- yl)piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
550.3


 


685




5-(3-(4-chlorophenyl)- 7-(4-(5- cyclopropyl-1,3,4- oxadiazo1-2- yl)piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
561.4


 


686




5-(7-((1S,4S)-5-benzyl-2,5- diazabicyclo[2.2.1]heptane-2- carbonyl)-3-(4- chlorophenyl)quinoxalin-2- yl)pentanoic acid
555.4


 


687




(S)-5-(3-(4-chlorophenyl)-7- ((1-(4-fluorophenyl)-2- hydroxyethyl)carbamoyl) quinoxalin-2-yl) pentanoic acid
522


 


688




(S)-5-(7-((1-(4-fluorophenyl)- 2-hydroxyethyl)carbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
488


 


689




5-(3-(4-chlorophenyl)-7-(3- hydroxy-3-phenylpyrrolidine- 1-carbonyl)quinoxalin-2- yl)pentanoic acid
530.4


 


690




5-(3-(4-chlorophenyl)-7-(1- phenyl-3- azabicyclo[3.1.0]hexane-3- carbonyl)quinoxalin-2- yl)pentanoic acid
526.4


 


691




5-(3-(4-chlorophenyl)-7-((2- oxo-1,2,3,4- tetrahydroquinolin-4- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
529.4


 


692




(S)-5-(3-(4-chlorophenyl)-7- ((7-(trifluoromethyl)chroman- 4-yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
584.4


 


693




5-(3-(4-chlorophenyl)-7-((2-(4- fluorophenyl)propan-2- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
520.4


 


694




5-(3-(4-chlorophenyl)-7-((6,8- difluorochroman-4- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
552.4


 


695




5-(3-(4-chlorophenyl)-7-(2- methyl-3-oxospiro[isoindoline- 1,4′-piperidin]-1′- ylcarbonyl)quinoxalin-2- yl)pentanoic acid
583.5


 


696




5-(3-(4-chlorophenyl)-7-((3- hydroxy-2,2-dimethylchroman- 4-yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
560.4


 


697




5-(3-(4-chlorophenyl)-7-(4-(2- (methylsulfonyl)phenyl) piperazine-1-carbonyl) quinoxalin-2- yl)pentanoic acid
607.5


 


698




5-(7-((1R,4R)-5-benzyl-2,5- diazabicyc1o[2.2.2]octane-2- carbonyl)-3-(4- chlorophenyl)quinoxalin-2- yl)pentanoic acid
569.5


 


699




5-(7-((1R,4R)-5- ((benzyloxy)carbonyl)-2,5- diazabicyclo[2.2.1] heptane-2- carbonyl)-3-(4- chlorophenyl)quinoxalin-2- yl)pentanoic acid
599.5


 


700




5-(3-(4-chlorophenyl)-7-(4- (1,2-dimethyl-1H- imidazol-4- yl)piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
547.5


 


701




5-(7-(4-(benzo[d]oxazol-2- yl)piperazine-1- carbonyl)-3-(4- chlorophenyl)quinoxalin-2- yl)pentanoic acid
570.5


 


702




5-(7-(1R,5S)-3-benzyl-3,8- diazabicyclo[3.2.1]octane-8- carbonyl)-3-(4- chloropheayl)quinoxalin-2- yl)pentanoic acid
569.5


 


703




5-(3-(4-chlorophenyl)-7-(4- (2,6-dimethylpyridin-4- yl)piperazine-1- carbonyl)quinoxalin-2- yl)pentanoic acid
558.5


 


704




5-(7-((1R,5S)-8-benzyl-3,8- diazabicyclo[3.2.1]octane-3- carbonyl)-3-(4- chlorophenyl)quinoxalin-2- yl)pentanoic acid
569.5


 


705




5-(3-(4-chlorophenyl)-7- (5,6,7,8- tetrahydroimidazo[1,2- a]pyrazine-7- carbonyl)quinoxalin-2- yl)pentanoic acid
490.3


 


706




2-hydroxy-5-(3-phenyl-7- (((R)-1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
496


 


707




5-(7-((4,4-dimethyl-1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)-3- phenylquinoxalin-2- yl)pentatioic acid
508


 


708




(R)-5-(7-((1,1- dioxidothiochroman-4- yl)carbamoyl)-3-(4- fluorophenyl)quinoxalin-2- yl)pentanoic acid
548


 


709




(S)-5-(7-((1,1- dioxidothiochroman-4- yl)carbamoyl)-3-(4- fluorophenyl)quinoxalin-2- yl)pentanoic acid
548


 


710




(S)-5-(7-((2-oxo-1,2,3,4- tetrahydroquinolin-4- yl)carbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
495


 


711




(R)-5-(7-((2-oxo-1,2,3,4- tetrahydroquinolin-4- yl)carbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
495


 


712




(S)-5-(7-((6-fluoro-2-oxo- 1,2,3,4-tetrahydroquinolin-4- yl)carbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
513


 


713




(R)-5-(7-((6-fluoro-2-oxo- 1,2,3,4-tetrahydroquinolin-4- yl)carbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
513


 


714




(S)-5-(7-((1,1- dioxidothiochroman-4- yl)carbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
530


 


715




(R)-5-(7-((1,1- dioxidothiochroman-4- yl)carbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
530


 


716




(R)-5-(6-fluoro-3-phenyl-7- ((1,2,3,4-tetrahydro- naphthalen- 1-yl)carbamoyl) quinoxalin-2- yl)pentanoic acid
498


 


717




(R)-5-(6-fluoro-3-(4- fluorophenyl)-7-((1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
516


 


718




(R)-5-(3-(4- chlorophenyl)-6- fluoro-7-((1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
532


 


719




5-(3-phenyl-7-(3-phenyl-8- azabicyclo[3.2.1]oct-2-ene-8- carbonyl)quinoxalin-2- yl)pentanoic acid
518.4


 


720




(R)-5-(3-(4-chlorophenyl)-7- ((7-(trifluoromethyl)chroman- 4-yl)carbamoyl)quinoxalin-2- yl)pentanoic acid
584.5


 


721




(S)-5-(7-((4,4-dimethyl- 1,2,3,4-tetrahydronaphthalen- 1-yl)carbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
508


 


722




(R)-5-(7-((4,4-dimethyl- 1,2,3,4-tetrahydronaphthalen- 1-yl)carbamoyl)-3- phenylquinoxalin-2- yl)pentanoic acid
508


 


723




(R)-4-(7-((6-fluoro-1,2,3,4- tetrahydronaphthalen-1- yl)carbamoyl)-3- phenylquinoxalin-2- yl)butanoic acid
484


 


724




(R)-4-(3-phenyl-7-((1,2,3,4- tetrahydronaphthalen-l- yl)carbamoyl)quinoxalin-2- yl)butanoic acid
466


 


725




(R)-4-(7-((7-fluorochroman-4- yl)carbamoyl)-3- phenylquinoxalin-2- yl)butanoic acid
486


 


726




4-(7-(4-(4- fluorophenyl)piperazine-1- carbonyl)-3-phenylquinoxalin- 2-yl)butanoic acid
500


 


727




(R)-5-(6-fluoro-7-((1-(4- fluorophenyl)ethyl) carbamoyl)- 3-phenylquinoxalin-2- yl)pentanoic acid
490


 


728




(R)-5-(7-(chroman-4- ylcarbamoyl)-6-fluoro-3- phenylquinoxalin-2- yl)pentanoic acid
500





*The left side 3-methyl-3-phenylpiperidine used to synthesize compounds 170 and 171 was prepared according to J. Org. Chem. 2007, 72, 4431






Biological Assays

Radioligand Binding Assay.

Radioligand binding assays were performed at room temperature in 50 mM Tris-HCl pH 7.4, 1 mM EDTA containing 2 mM MnCl2 and 3.0 nM [3H]PGD2 (New England Nuclear, Boston, Mass.) (171 Ci mmol−1), in a final volume of 0.2 mL. Competing ligands were diluted in dimethylsulfoxide (Me2SO) that was kept constant at 1% (v/v) of the final incubation volume. The reaction was initiated by the addition of 8-20 μg of membrane protein prepared from a human embryonic kidney (HEK)-hCRTH2 cell line. Total and non-specific binding were determined in the absence and the presence of 10 μM PGD2, respectively. Under these conditions, specific binding (total minus non-specific) of the radioligand to the receptor reached equilibrium within 50 min and was stable up to 180 min. The reaction was routinely conducted for 60 min at room temperature and terminated by rapid filtration through prewetted (0.3% polyethyleneimine) 96-well printed FiltermateTM (Wallac) using a Tomtec® harvester (Hamden, Conn.). After washing with cold buffer, the filter was dried for 2 minutes in microwave, and Meltilex Scintillator sheet (Wallac) was melted on for 2 min. The radioactivity was measured with Betaplate model 1205 (Wallac). Tables A and B below list representative compounds of the invention with binding data whereby the Ki values are rated “A”, “B,” “C,” “D,” or “E.” The Ki values are rated “A” for Ki values in the range of 0.1 to 2.0 nM, “B” for Ki values in the range from 2.1-20 nM, “C” for Ki values in the range from 20.1-200 nM, “D” for Ki values in the range from 201-700 nM, and “E” for Ki values in the range from 701-2300 nM. The designation “NT” indicates that the compound in the entry was not tested in this binding assay.










TABLE A







No.
Ki (nM)









3
B



3T
C



3U
C



3V
D



3W
B



3X
B



3Y
A



3Z
A



3AA
B



3AB
B



3AC
B



3AD
B



3AE
B



3AF
B



3AG
B



4
B



4D
C



4E
A



4F
B



4G
B



5
A



5G
A



5N
C



5O
C



5P
D



5Q
C



5R
B



5S
B



6
A



6F
NT



6G
A



6H
B



7
B



7C
A



8
C



8G
C



9
B



10
B



10C
A



10D
B



10E
A



10F
B



11
C



11D
D



11E
D



11F
D



12
D



13
C



14
B



14C
C



14D
B



14E
C



14F
C



14G
A



14H
C



15A
B



15B
B



15C
A



15D
A



15E
A



15F
B



15G
B



15H
C



15i
D



15J
B



15K
B



15L
C



15M
C



15N
B



15o
B



15P
A



15Q
A



15R
A



15S
A



15T
A



15U
B



15V
A



15X
A



15Y
A



15Z
A



15AA
C



15AB
A



15AC
A



15AD
B



15AE
B



15AF
B



15AG
B



15AH
C



15Ai
A



15AJ
B



15AK
B



15AL
A



15AM
B



15AN
B



15Ao
A



15AP
B



15AQ
B



15AR
A



15AS
B



15AT
A



15AU
B



15AV
A



15AW
A



15AX
B



15AY
B



15AZ
B



15BA
A



15BB
B



15BC
B



15BD
B



15BE
B



15BF
B



15BG
B



15BH
B



15Bi
A



15BJ
B



15BK
A



15BL
C



15BM
B



15BN
B



15Bo
C



15BQ
B



15BR
A



15BS
B



15BT
A



15BU
A



16
A



16F
A



16G
A



16H
A



16i
A



16J
B



16K
A



16L
B



16M
A



16N
A



16o
A



16P
A



16Q
A



16R
A



16S
A



16T
A



16U
A



16V
A



16W
A



16X
B



16Y
A



16Z
B



16AA
A



16AB
A



16AC
A



16AD
A



16AE
A



16AF
A



16AG
A



16AH
B



16Ai
A



16AJ
A



16AK
A



16AL
A



16AM
A



16AN
A



16Ao
A



16AP
A



16AQ
A



16AR
B



16AS
A



17
B



17D
A



17E
B



17F
A



17G
B



17H
B



17i
B



17J
B



17K
A



17L
B



17M
B



17N
B



17o
A



17P
B



17Q
B



17R
A



17S
B



17T
A



17U
B



17V
A



17W
A



17X
B



17Y
B



17Z
B



18
B



18D
B



18E
A



18F
B



18G
A



18H
B



19
B



19F
B



19G
B



19H
B



19i
B



19J
C



19K
B



20
C



20F
B



20G
B



20H
B



21
A



21C
B



22
C



22D
C



23
C



24
B



24H
B



24i
B



25
D



26
A



26E
B



26F
B



26G
B



26H
B



26i
B



26J
A



26K
B



26L
B



26M
B



26N
A



26o
A



26P
B



26Q
C



26R
C



26S
A



26T
B



26U
B



26V
A



26W
A



26X
C



26Y
A



26Z
A



26AA
A



26AB
A



28
B



29
B



30
B



30E
B



31
B



31C
B



32
B



33
C



34
D



35
C



36
E



37
C



38
C



38A
C



38B
C



39
B



40
B



41
B



42
B



44
B



44D
B



44E
B



44F
B



44G
C



44H
B



44i
A



44J
B



44K
A



44L
B



44M
B



44N
B



100
A



101
B



102
B



103
A



104
B



105
B



106
A



107
B



108
B



109
B



110
B



111
B



112
B



113
B



114
B



115
C



116
B



117
B



118
B



119
B



120
B



121
B



122
B



123
B



124
B



125
B



126
B



127
B



128
B



129
A



130
A



131
B



132
B



133
B



134
A



135
B



136
B



137
C



138
B



139
B



140
A



141
B



142
B



143
B



144
C



145
B



146
C



147
B



148
A



149
B



150
A



151
A



152
B



153
A



154
A



155
B



156
B



157
B



158
D



159
D



160
B



161
B



162
A



163
B



164
B



165
B



166
B



167
B



168
B



169
B



170
B



171
B



172
A



173
A



174
A



175
B



176
B



177
B



178
A



179
A



180
A



181
A



182
B



183
B



184
A



185
B



186
A



187
B



188
A



189
A



190
A



191
A



192
B



193
B



194
C



195
C



196
C



197
D



198
B



199
B



200
B



201
A



202
A



203
B



204
B



205
B



206
B



207
B



208
B



209
B



210
B



211
B



212
B



213
C



214
A



215
B



216
C



217
A



218
A



219
A



220
A



221
A



222
B



223
A



224
A



225
A



226
A



227
A



228
A



229
A



230
A



231
B



232
B



233
B



234
B



235
B



236
B



237
B



238
B



239
B



240
B



241
B



242
B



243
B



244
B



245
B



246
A



247
B



248
B



249
A



250
A



251
A



252
A



253
A



254
A



255
A


















TABLE B





No.
Ki (nM)







256
A


257
B


258
B


259
D


260
B


261
B


262
B


263
B


264
B


265
B


266
B


267
B


268
B


269
D


270
D


271
D


272
C


273
C


274
B


275
C


276
B


277
B


278
C


279
C


280
E


281
C


282
D


283
D


284
B


285
B


286
C


287
C


288
C


289
C


290
B


291
B


292
C


293
B


294
B


295
B


296
B


297
B


298
B


299
B


300
B


301
B


302
B


303
B


304
B


305
B


306
C


307
C


308
B


309
B


310
B


311
B


312
B


313
B


314
B


315
B


316
B


317
B


318
B


319
B


320
B


321
B


322
B


323
B


324
B


325
C


326
B


327
C


328
B


329
B


330
B


331
B


332
B


333
B


334
B


335
B


336
C


337
C


338
B


339
C


340
B


341
B


342
B


343
B


344
B


345
B


346
B


347
C


348
B


349
C


350
C


351
B


352
B


353
B


354
C


355
B


356
C


357
B


358
B


359
B


360
C


361
B


362
B


363
B


364
B


365
B


366
B


367
B


368
C


369
B


370
D


371
B


372
B


373
B


374
A


375
A


376
B


377
B


378
C


379
B


380
B


381
A


382
A


383
A


384
A


385
B


386
A


387
B


388
B


389
A


390
A


391
B


392
B


393
B


394
B


395
B


396
B


397
B


398
B


399
B


400
B


401
C


402
B


403
A


404
B


405
B


406
B


407
B


408
A


409
C


410
C


411
C


412
A


413
A


414
A


415
A


416
B


417
B


418
C


419
A


420
A


421
A


422
B


423
B


424
B


425
A


426
A


427
B


428
C


429
A


430
B


431
B


432
A


433
B


434
A


435
A


436
C


437
C


438
B


439
B


440
A


441
B


442
A


443
B


444
C


445
B


446
B


447
A


448
A


449
B


450
B


451
B


452
B


453
B


454
B


455
B


456
B


457
A


458
B


459
B


460
B


461
B


462
B


463
B


464
C


465
B


466
B


467
B


468
B


469
D


470
C


471
B


472
C


473
B


474
B


475
B


476
C


477
B


478
C


479
C


480
B


481
C


482
B


483
B


484
B


485
B


486
B


487
C


488
B


489
B


490
B


491
A


492
B


493
B


494
B


495
B


496
B


497
B


498
B


499
B


500
B


501
C


502
C


503
C


504
B


505
B


506
A


507
A


508
B


509
C


510
C


511
B


512
A


513
A


514
B


515
B


516
B


517
B


518
B


519
B


520
B


521
C


522
B


523
B


524
B


525
C


526
D


527
B


528
C


529
C


530
C


531
B


532
B


533
B


534
B


535
B


536
B


537
B


538
B


539
B


540
B


541
B


542
B


543
C


544
C


545
D


546
C


547
C


548
D


549
B


550
B


551
B


552
B


553
B


554
B


555
B


556
B


557
B


558
B


559
A


560
A


561
A


562
A


563
A


564
B


565
A


566
B


567
A


568
B


569
A


570
B


571
B


572
C


573
B


574
B


575
A


576
B


577
A


578
A


579
B


580
A


581
A


582
A


583
A


584
B


585
A


586
B


587
A


588
B


589
C


590
B


591
B


592
B


593
A


594
D


595
A


596
A


597
B


598
B


599
A


600
A


601
B


602
A


603
B


604
A


605
A


606
B


607
A


608
B


609
A


610
A


611
B


612
A


613
C


614
B


615
B


616
C


617
A


618
A


619
A


620
A


621
A


622
B


623
B


624
A


625
B


626
B


627
A


628
A


629
A


630
B


631
A


632
A


633
A


634
B


635
A


636
A


637
B


638
D


639
C


640
B


641
B


642
B


643
A


644
B


645
B


646
B


647
C


648
B


649
C


650
C


651
C


652
B


653
D


654
C


655
B


656
B


657
C


658
C


659
C


660
C


661
B


662
C


663
B


664
B


665
B


666
B


667
B


668
C


669
B


670
B


671
C


672
C


673
C


674
B


675
B


676
B


677
B


678
B


679
B


680
B


681
B


682
C


683
B


684
B


685
C


686
C


687
B


688
C


689
B


690
B


691
B


692
B


693
B


694
B


695
B


696
B


697
B


698
B


699
B


700
C


701
B


702
B


703
D


704
B


705
D


706
D


707
B


708
D


709
B


710
C


711
B


712
C


713
B


714
C


715
B


716
B


717
B


718
B


719
B


720
B


721
B


722
B


723
C


724
D


725
D


726
D


727
B


728
B









Representative compounds of the invention had the Ki values specified in parentheses immediately following the compound number in the above-described assay: 3 (3.2 nM), 5P (265.6 nM), 6G (0.5 nM), 11F (222.9 nM), 15H (152.4 nM), 15i (282.9 nM), 15o (16.7 nM), 15Ai (1.9 nM), 15Ao (1.0 nM), 15AR (1.6 nM), 15AX (3.0 nM), 15BL (69.9 nM), 15BR (0.1 nM), 16S (0.7 nM), 16V (2.7 nM), 16W (0.4 nM), 16AG (1.2 nM), 16Ao (0.4 nM), 26o (1.4 nM), 26Q (96.7 nM), 36 (716.1) 39 (8.0 nM), 40 (5.7 nM), 41 (8.8 nM), 44H (3.1 nM) 144 (117.9 nM), 146 (132.0 nM), 152 (2.9 nM), 159 (564.4 nM), 173 (1.6 nM), 180 (0.6 nM), 193 (14.6 nM), 197 (464.1 nM), 217 (1.2 nM), 231 (4.0 nM), 246 (1.9 nM), 251 (1.0 nM), 254 (1.2 nM), 255 (1.5 nM), 264 (15.4 nM), 292 (39.2 nM), 368 (21.4 nM), 438 (4.2 nM), 507 (1.3 nM), 526 (259.3 nM), 637 (4.2 nM), 657 (37.4 nM), and 709 (18.4 nM).
i[cAMP] Measurements.
The ability of the compounds to antagonize the formation of cAMP can be assayed using the ELISA-based assay described in this example. HEK-hCRTH2 cells are grown to 80-90% confluency. On the day of the assay, the cells are washed with phosphate buffered saline (PBS), incubated for 2 min in cell dissociation buffer, harvested by centrifugation at 300 g for 7 min at room temperature and resuspended at 1.25e106 cells mL−1 in Hanks' balanced salt solution containing 20 mM HEPES pH 7.4 and 0.75 mM IBMX (HBSS/HEPES/IBMX). The assay is performed in 384-plate format with 0.01 mL HBSS/HEPES/IBMX per well containing 12 500 cells and 70 to 75 n1 of the test compound and DK-PGD2 at various concentrations. Following a 0 to 10 to min pre-incubation of the cells with the test compound at 37° C., 0.005 mL of 30 μM Forskolin dilute in HBSS 20 mM HEPES, is added at a final concentration of 10 uM to initiate the reaction. After 10 to 60 min incubation at room temperature or 37° C., the cAMP content was quantified using the cAMP XS+ HitHunter chemiluminescence assay (GE Healthcare 90-0075). Percent inhibition is calculated using the Forskolin and EC85 DK-PGD2 controls.
β-Arrestin Assay:
CHO-K1 cells obtained from DiscoverX are stably transfected with human CRTH2 (propagation medium: F-12, 10% FBS, 300 ug/mL hygB and 800 ug/mL G418). Cells are grown in T175 cm2 flask. While in log phase, cells are collected via 0.05% trypsin treatment. Triturated cells are filtered and 40 uL (10K cells) are plated per well in a 384-well white clear bottom plate and incubated O/N. Cell plate is emptied via inversion and blotted dry. Each well is filled with 35 uL of HBSS (with Ca++ and Mg++) and incubated for 5 min. Compounds are added in volumes of 1 μL and the plate is gently shaken for 2 min., followed by incubation at 37° C. for 20 min. All compounds and controls are diluted in HBSS assay buffer (with Ca++ and Mg++) with a final concentration range of 10−5M to 3×10−11 M, 11 point Dose response curves at appropriate half-log increments. Final DMSO % is ≦0.3%. Agonist Assay: 1 μl/well of compound is added into cell plate and left to incubate at 37° C. for 90 min. Antagonist Assay: 1 μl/well of compounds are added into a cell plate. Incubate 30 minutes at 37° C. Stimulate cells with 1 ul/well of PGD2 [100 nM] final. Incubate plate for 60 minutes at 37° C. Resulting luminescent signal is detected via Discoverx PathHunter Detection Kit per manufacturer's instructions. A total of 12 μl/well is added to each well. The plate is covered and incubated for 60 min. with gentle shaking. Chemiluminescent detection is done by a SpectraMax plate reader.
Eosinophil Shape Change Assay in Human Whole Blood:
Blood is collected in vacutainers containing EDTA. The antagonist is added to blood and incubated for 10 min at room temperature. DK-PGD2 (13,14-dihydro-15-keto prostaglandin D2) are then added to blood for 4 min at 37° C. in a running water bath. Blood cells are then fixed in presence of cold 0.25% (v/v) paraformaldehyde prepared in 75% (v/v) DPBS without Ca++ and Mg++ for 1 min on ice. 175 μL of fixed blood is transferred into 870 μL of cold 155 mM NH4Cl lysis solution and incubated at 4° C. for at least 40 min. The solution is then centrifuged at 430 g for 5 min and the supernatant is discarded. Centrifuged cells are resuspended in residual supernatant and sodium azide is added (1% final concentration). Samples are analyzed with a FACs Calibur flow cytometer (Becton Dickinson). Flow cytometry raw data is analyzed with Diva software by isolating the eosinophils from the neutrophils based on their high autofluorescence and determining the percent of total eosinophils with increased forward light scatter. Maximum (100%) and minimum (0%) shape change is determined in the presence of 10 μM DK-PGD2 and DPBS, respectively. A dose response curve with DK-PGD2 is performed with every assay to determine the EC50 for each blood donor. Compounds are tested in 11-dose titration curves in the presence of 50 nM DK-PGD2 to determine an antagonist IC50.
Compounds of the present invention are selective for the CRTH2 receptor over the DP receptor. Assays on the DP, as well as other prostanoid, receptors are described in WO2003/06220.
While the present invention has been described in conjunction with the specific embodiments set forth above, many alternatives, modifications and other variations thereof will be apparent to those of ordinary skill in the art. All such alternatives, modifications and variations are intended to fall within the spirit and scope of the present invention.

## Claims

1. A compound of the formula (I)



or a pharmaceutically acceptable salt thereof, wherein
J1 and J2 are independently C(H), C(R1), C(R22), or N wherein the following provisos apply
(i) no more than one of J1 and J2 is N,
(ii) no more than one of J1 and J2 is C(R22); and
(iii) only one R1 is substituted on the illustrated ring containing J1 and J2;

R1 is selected from the group consisting of
(i) —C(O)—N(R6a)(R6b),
(ii) —S(O)2—N(R6a)(R6b),
(iii) —C(O)—C(R7a)(R7b)(R7c),
(iv) —N(H)—C(O)—C(R7a)(R7b)(R7c),
(v) —C(O)—O—C(R7a)(R7b)(Rc), and
(vi) —N(H)—S(O)2—C(R7a)(R7b)(R7c);

R6a and R6b are independently
a. H,
b. C1-C6 alkyl,
c. C3-C6 alkenyl,
d. C3-C6 alkynyl,
e. —O—(C1-C3 alkyl),
f. -Q-RAH, wherein RAH is phenyl or 5- to 6-membered heteroaryl containing 1 to 2 heteroatoms independently selected from the group consisting of N, O, and S,
and wherein RAH is unsubstituted or substituted with 1 to 5 R8 moieties independently selected from the group consisting of halo, C1-C3 alkyl, C1-C3 alkoxy, C1-C3 fluoroalkyl, —O—(C1-C3 fluoroalkyl), hydroxyl, phenyl, and —CN;

Q is selected from the group consisting of
(a) a bond;
(b) C1-C6 alkylene, wherein said C1-C6 alkylene is unsubstituted or substituted by 1 to 2 fluoro, C1-C3 alkyl, C1-C3 hydroxyalkyl, or C1-C3 fluoroalkyl; and
(c)








 wherein t is 1, 2, 3, or 4;

g. -Q-RHc, wherein RHC is
(i) 5- to 7-membered heterocyclyl containing 1 heteroatom selected from the group consisting of N, O, S, S(O), and S(O)2, wherein said heterocyclyl of RHC is optionally fused to a benzene, pyridyl ring; or
(ii) C3-C7 cycloalkyl, wherein said cycloalkyl of RHC is optionally fused to a benzene or pyridyl ring;
and wherein RHC is unsubstituted or substituted with 1 to 5 R12 moieties independently selected from the group consisting of halo, C1-C3 alkyl, C1-C3 alkoxy, C1-C3 fluoroalkyl,
—O—(C1-C3 fluoroalkyl), hydroxyl, —CN, and —S(O)2—(C1-C3 alkyl), or wherein when two R12 moieties are geminally substituted on the same carbon atom, the two geminally substituted R12 moieties, together with the carbon atom on which they are attached form —C(O)—;


Q is selected from the group consisting of
(a) a bond;
(b) C1-C6 alkylene, wherein said C1-C6 alkylene is unsubstituted or substituted by 1 to 2 fluoro, C1-C3 alkyl, C1-C3 hydroxyalkyl, or C1-C3 fluoroalkyl; and








 wherein t is 1, 2, 3, or 4;

h. or R6a and R6b together with the N atom to which they are attached form R6H, wherein R6H is independently selected from the group consisting of
(i) a 4- to 9-membered heterocyclyl, optionally containing one additional nitrogen atom, wherein said heterocyclyl of R6H is optionally fused to phenyl, C3-C6 cycloalkyl, or a 5-membered heteroaryl containing 1 to 3 N atoms;
(ii) a 4- to 7-membered heterocyclenyl, optionally containing one additional nitrogen atom, wherein said heterocyclenyl of R6H is optionally fused to phenyl; and
(iii) a 6- to 8-membered aza- or a diazabicycloheterocycloalkyl ring;
wherein R6H is unsubstituted or substituted by 1 to 5 R9 moieties wherein each R9 moiety is independently C1-C6 alkyl, C1-C6 alkoxy, C1-C3 fluoroalkyl, fluoro, hydroxyl, —CN,
—(C1-C3 alkylene)-(C1-C3 alkoxy), or
R9 is —Z—RCY wherein
 Z is
 (i) a bond,
 (ii) —C(O)—,
(iii) —C(═N—OH)—,
 (iv) —S(O)2—,
 (v) C1-C3 alkylene, wherein said C1-C3 alkylene of Z is optionally substituted by 1 to 2 fluoro or C1-C3 alkyl;
 (vi) —O—;
 (vii) —O—(C1-C3 alkylene)-; or
 (viii) —C(O)—O—CH2—
 RCY is selected from the group consisting of
 (i) phenyl
 (ii) 5- to 10-membered mono or bicyclic heteroaryl containing 1 to 3 heteroatoms independently selected from the group consisting of N, O, and S; or
 (iii) 5- to 6-membered heterocyclyl containing 1 to 2 N atoms or 1 O atom, wherein said heterocyclyl of RCY is optionally fused to phenyl;
 wherein RCY is unsubstituted or substituted by 1 to 4 RH) moieties;
 each R10 moiety is independently C1-C3 alkyl, halo, hydroxyl, C1-C3 alkoxy, C1-C3 fluoroalkyl, —(C1-C3 alkylene)-(C1-C3 alkoxy), —S(O)2—(C1-C3 alkyl), —C(O)—(C1-C3 alkyl), —CN, pyridyl, or cyclopropyl or, wherein when two R10 moieties are geminally substituted on a common carbon atom, together with the carbon atom on which they are substituted, form —C(O)—;
or, optionally, where two R9 moieties are geminally substituted on a common ring carbon of R6H, the two R9 moieties, together with the ring carbon on which they are substituted, form RYC, wherein RYC is
 (i) a 4- to 7-membered cycloalkyl, wherein said cycloalkyl of RYC is optionally fused to phenyl or pyridyl; or
 (ii) a 4- to 7-membered heterocyclyl containing 1 to 2 N atoms or 1 O atom, wherein said heterocyclyl of RYC is optionally fused to phenyl;
 wherein RYC is unsubstituted or substituted by 1 to 4 R11 moieties;
 each R11 moiety is independently C1-C3 alkyl, halo, hydroxyl, C1-C3 alkoxy, —(C1-C3 alkylene)-(C1-C3 alkoxy), —S(O)2—(C1-C3 alkyl), —C(O)—(C1-C3 alkyl), phenyl, or pyridyl, or, wherein when two R11 moieties are geminally substituted on a common carbon atom, together with the carbon atom on which they are substituted, form —C(O)—;


R7a and R7b are independently
a) H,
b) C1-C6 alkyl,
c) R7a and R7b together with the carbon atom on which they are substituted, form RPC, wherein RPC is
(i) C3-C7 cycloalkyl, or
(ii) phenyl, wherein said phenyl of RPC is unsubstituted or substituted by 1 to 5 moieties independently selected from the group consisting of halo, trifluoromethyl, and trifluoromethoxy;


R7c is
a) H, or
b) absent, when R7a and R7b together with the carbon atom on which they are substituted form phenyl;


R22 is halo, C1-C3 alkyl, or C1-C3 fluoroalkyl;
b is 0 or 1;
X is a bond, —O—, —S—, —S(O)—, —S(O)2—, or N(H);
Y is selected from the group consisting of
(i) a bond,






 (iv) —O—, (v) —S—, (vi) —S(O)—, and
(vii) —S(O)2—;
wherein
a is 0, 1, 2, or 3;
r is 0, 1, or 2;
s is 0, 1, or 2;

each occurrence of R4 is independently halo, C1-C6 alkyl, or C1-C6 fluoroalkyl;

Ra, Rb, Rc, and Rd are independently H, fluoro, hydroxyl, C1-C6 alkyl, C1-C6 fluoroalkyl, or C1-C6 alkoxy;
R2 is selected from the group consisting of
(i) phenyl; and
(ii) 5- to 6-membered heteroaryl containing from 1 to 3 heteroatoms selected from the group consisting of N, O, and S;
wherein R2 is unsubstituted or substituted by 1 to 5 R5 groups independently selected from the group consisting of halo, C1-C3 fluoroalkyl, C1-C3 alkyl, C1-C3 alkoxy, —CN, —OCF3, —C(O)—(C1-C3 alkyl), and —S(O)2—(C1-C3 alkyl);

R3 is —C(O)OH,




 or —N(H)—SO2—Re,
wherein Re is C1-C6 alkyl, C1-C6 fluoroalkyl, C1-C6 alkoxy, and phenyl;

n is 1, 2, 3, 4, or 5; and
q is 0, 1, or 2.

2. The compound of claim 1 or a pharmaceutically acceptable salt thereof, wherein R1 is selected from the group consisting of:
(i) —C(O)—N(R6a)(R6b),
(ii) —S(O)2—N(R6a)(R6b),
(iii) —C(O)—C(R7a)(R7b)(R7c),
(iv) —N(H)—C(O)—C(R7a)(R7b)(R7c),
(v) —C(O)—O—C(R7a)(R7b)(R7c), and
(vi) —N(H)—S(O)2—C(R7a)(R7b)(R7c);
R6a and R6b are independently:
a. H,
b. C1-C6 alkyl,
c. C3-C6 alkenyl,
d. C3-C6 alkynyl,
e. —O—(C1-C3 alkyl),
f. -Q-RAH, wherein RAH is phenyl or 5- to 6-membered heteroaryl containing 1 to 2 heteroatoms independently selected from the group consisting of N, O, and S,
and wherein RAH is unsubstituted or substituted with 1 to 5 R8 moieties independently selected from the group consisting of halo, C1-C3 alkyl, C1-C3 alkoxy, C1-C3 fluoroalkyl, —O—(C1-C3 fluoroalkyl), hydroxyl, phenyl, and —CN;

Q is selected from the group consisting of
(a) a bond;
(b) C1-C6 alkylene, wherein said C1-C6 alkylene is unsubstituted or substituted by 1 to 2 fluoro, C1-C3 alkyl, C1-C3 hydroxyalkyl, or C1-C3 fluoroalkyl; and
(c)








 wherein t is 1, 2, 3, or 4;

g. -Q-RHC, wherein RHC is
(i) 5- to 6-membered heterocyclyl containing 1 heteroatom selected from the group consisting of N and O, wherein said heterocyclyl of RHC is optionally fused to a benzene ring; or

(ii) C5-C7 cycloalkyl, wherein said cycloalkyl of RHC is optionally fused to a benzene ring;
and wherein RHC is unsubstituted or substituted with 1 to 5 R12 moieties independently selected from the group consisting of halo, C1-C3 alkyl, C1-C3 alkoxy, C1-C3 fluoroalkyl,
—O—(C1-C3 fluoroalkyl), hydroxyl, and —CN, or wherein when two R12 moieties are geminally substituted on the same carbon atom, the two geminally substituted R12 moieties, together with the carbon atom on which they are attached form —C(O)—;

Q is selected from the group consisting of
(a) a bond;
(b) C1-C6 alkylene, wherein said C1-C6 alkylene is unsubstituted or substituted by 1 to 2 fluoro, C1-C3 alkyl, C1-C3 hydroxyalkyl, or C1-C3 fluoroalkyl; and
(c)








 wherein t is 1, 2, 3, or 4;

h. or R6a and R6b together with the N atom to which they are attached form R6H, wherein R6H is independently selected from the group consisting of
(i) a 4- to 7-membered heterocyclyl, optionally containing one additional nitrogen atom, wherein said heterocyclyl of R6H is optionally fused to phenyl; and
(ii) a 4- to 7-membered heterocyclenyl, optionally containing one additional nitrogen atom, wherein said heterocyclenyl of R6H is optionally fused to phenyl;
wherein R6H is unsubstituted or substituted by 1 to 5 R9 moieties wherein each R9 moiety is independently C1-C6 alkyl, C1-C6 alkoxy, fluoro, hydroxyl, —CN, —(C1-C3 alkylene)-(C1-C3 alkoxy), or
R9 is —Z—RCY wherein
 Z is
 (i) a bond,
 (ii) —C(O)—,
 (iii) —C(═N—OH)—,
 (iv) —S(O)2—, or
 (v) C1-C3 alkylene, wherein said C1-C3 alkylene of Z is optionally substituted by 1 to 2 fluoro or C1-C3 alkyl;
 RCY is selected from the group consisting of
 (i) phenyl
 (ii) 5- to 10-membered mono or bicyclic heteroaryl containing 1 to 3 heteroatoms independently selected from the group consisting of N, O, and S; or
 (iii) 5- to 6-membered heterocyclyl containing 1 to 2 N atoms or 1 O atom, wherein said heterocyclyl of RCY is optionally fused to phenyl;
 wherein RCY is unsubstituted or substituted by 1 to 4 R10 moieties;
 each R10 moiety is independently C1-C3 alkyl, halo, hydroxyl, C1-C3 alkoxy, —(C1-C3 alkylene)-(C1-C3 alkoxy), —S(O)2—(C1-C3 alkyl), —C(O)—(C1-C3 alkyl), —CN, or pyridyl, or, wherein when two R10 moieties are geminally substituted on a common carbon atom, together with the carbon atom on which they are substituted, form —C(O)—;
or, optionally, where two R9 moieties are geminally substituted on a common ring carbon of R6H, the two R9 moieties, together with the ring carbon on which they are substituted, form RYC, wherein RYC is
 (i) a 4- to 7-membered cycloalkyl, wherein said cycloalkyl of RYC is optionally fused to phenyl; or
 (ii) a 4- to 7-membered heterocyclyl containing 1 to 2 N atoms or 1 O atom, wherein said heterocyclyl of RYC is optionally fused to phenyl;
 wherein RYC is unsubstituted or substituted by 1 to 4 R11 moieties;
 each R11 moiety is independently C1-C3 alkyl, halo, hydroxyl, C1-C3 alkoxy, —(C1-C3 alkylene)-(C1-C3 alkoxy), —S(O)2—(C1-C3 alkyl), —C(O)—(C1-C3 alkyl), phenyl, or pyridyl, or, wherein when two R11 moieties are geminally substituted on a common carbon atom, together with the carbon atom on which they are substituted, form —C(O)—;


R7a and R7b independently
a) H,
b) C1-C6 alkyl,
c) R7a and R7b together with the carbon atom on which they are substituted, form RPC, wherein RPC is
(i) C3-C7 cycloalkyl, or
(ii) phenyl, wherein said phenyl of RPC is unsubstituted or substituted by 1 to 5 moieties independently selected from the group consisting of halo, trifluoromethyl, and trifluoromethoxy;


R7c is
a) H, or
b) absent, when R7a and R7b together with the carbon atom on which they are substituted form phenyl; and


X is a bond, —O—, —S—, —S(O)—, or —S(O)2—;
J1, J2, Y, Ra, Rb, Rc, Rd, R2, R3, R22, b, n, and q are as specified in claim 1.

3. The compound of claim 1 or a pharmaceutically acceptable salt thereof, wherein R1 is substituted at the 6 or 7 position of the illustrated bicyclic ring of Formula (I).

4. The compound of claim 3 or a pharmaceutically acceptable salt thereof, wherein the compound has the formula (IA)



wherein J1 and J2 are independently C(H) or C(R22), or N wherein the following provisos apply
(i) no more than one of J1 and J2 is N, and
(ii) no more than one of J1 and J2 is C(R22).

5. The compound of claim 1, or a pharmaceutically acceptable salt thereof, wherein R1 is selected from the group consisting of:
(i) —C(O)—N(R6a)(R6b), and
(ii) —S(O)2—N(R6a)(R6b).

6. The compound of claim 5, or a pharmaceutically acceptable salt thereof, wherein
(i) R6a is H and
R6b is -Q-RAH or -Q-RHC; or

(ii) R6a and R6b together with the N atom to which they are attached form R6H.

7. The compound of claim 5, or a pharmaceutically acceptable salt thereof, wherein R1 is —C(O)—N(R6a)(R6b).

8. The compound of claim 7, or a pharmaceutically acceptable salt thereof, wherein
R6a is H and
R6b is -Q-RAH or -Q-RHC.

9. The compound of claim 7, or a pharmaceutically acceptable salt thereof, wherein R6a and R6b together with the N atom to which they are attached form R6H.

10. The compound of claim 1, or a pharmaceutically acceptable salt thereof, wherein Y is selected from the group consisting of
(i) a bond,

11. The compound of claim 1, or a pharmaceutically acceptable salt thereof, wherein the group



is selected from the group consisting of

12. The compound of claim 1 or a pharmaceutically acceptable salt thereof, wherein the compound of the Formula (I) has the Formula (IB)



wherein
J1 is C(H) or N;
R1 is —C(O)—N(R6a)(R6b);
I) R6a is H and R6b is:
a) -Q-RAH, wherein RAH is phenyl or pyridyl,
and wherein RAH is unsubstituted or substituted with 1 to 2 R8 moieties independently selected from the group consisting of halo, C1-C3 alkyl, C1-C3 alkoxy, trifluoromethyl, trifluoromethoxy, and —CN;
Q is selected from the group consisting of
(i) a bond;
(ii)










 wherein Re and Rf are independently H, C1-C3 alkyl, or trifluoromethyl;
(iii) C2-C4 alkylene, wherein said C2-C6 alkylene is unsubstituted;

b) -Q-RHC, wherein RHC is
(i) 5- to 6-membered heterocyclyl containing 1 heteroatom selected from the group consisting of N and O; or
(ii) C5-C7 cycloalkyl, wherein said C5-C7 cycloalkyl is optionally fused to a benzene ring;
and wherein RHC is unsubstituted or substituted with 1 to 2 R12 moieties independently selected from the group consisting of C1-C3 alkyl, halo, and hydroxyl, or wherein when two R12 moieties are geminally substituted on the same carbon atom, the two geminally substituted R12 moieties, together with the carbon atom on which they are attached form —C(O)—;


II) or R6a and R6b together with the N atom to which they are attached form R6H, wherein R6H is independently selected from the group consisting of
a) a 4- to 6-membered heterocyclyl, optionally containing one additional nitrogen atom, wherein said 4- to 6-membered heterocyclyl is optionally fused to phenyl; and
b) a 5- to 6-membered heterocyclenyl, optionally containing one additional nitrogen atom, wherein said 4- to 6-membered heterocyclyl is optionally fused to phenyl;
wherein R6H is unsubstituted or substituted by 1 to 2 R9 moieties wherein each R9 moiety is independently C1-C3 alkyl, F, Cl,
 —CN, or
 R9 is —Z—RCY, wherein
 Z is a bond or —CH2—;
 RCY is selected from the group consisting of
 (i) phenyl;
 (ii) 5- to 6-membered heteroaryl containing 1 to 3 N atoms; or
 (iii) 5- to 6-membered heterocyclyl containing 2 N atoms, wherein said 5- to 6-membered heterocyclyl of RCY is fused to phenyl;
 wherein RCY is unsubstituted or substituted by 1 to 2 R10 moieties;
 each R10 moiety is independently C1-C3 alkyl, halo, C1-C3 alkoxy, —(C1-C3 alkylene)-(C1-C3 alkoxy), or —CN, or, wherein two R10 moieties are geminally substituted on a common carbon atom, together with the carbon atom on which they are substituted, form —C(O)—;
 or, optionally, where two R9 moieties are geminally substituted on a common ring carbon of R6H, the two R9 moieties, together with the ring carbon on which they are substituted, form RYC, wherein RYC is
 (i) a 5- to 6-membered cycloalkyl, wherein said or 5- to 6-membered cycloalkyl is fused to phenyl; or
 (ii) a 4- to 6-membered heterocyclyl containing 1 to 2 N atoms or 1 O atom, wherein said or 4- to 6-membered heterocyclyl is optionally fused to phenyl;
 wherein RYC is unsubstituted or substituted by 1 to 3 RH moieties;
 each R11 moiety is independently C1-C3 alkyl, —C(O)—(C1-C3 alkyl), or phenyl, or, wherein two R11 moieties are geminally substituted on a common carbon atom, together with the carbon atom on which they are substituted, form —C(O)—;



Y is selected from the group consisting of
(i) a bond,





Rc is H or methyl;
Rd is H;
R2 is phenyl, pyridyl, or thienyl;
wherein R2 is unsubstituted or substituted by 1 to 2 R5 groups independently selected from the group consisting of fluoro, chloro, trifluoromethyl, C1-C3 alkoxy, —CN, and —OCF3; and

q is 0, 1, or 2.

13. The compound of claim 1 or a pharmaceutically acceptable salt thereof, wherein the compound of the Formula (I) has the Formula (IC)



wherein
R1 is —C(O)—N(R6a)(R6b);
I) R6a is H and R6b is:
a) -Q-RAH, wherein RAH is phenyl,
and wherein RAH is unsubstituted or substituted with one R8 moiety selected from the group consisting of halo and —CN;
Q is selected from the group consisting of
(i) a bond;
(ii)










 wherein Re is H, and Rf is H or methyl;

b) -Q-RHC, wherein RHC is C5-C6 cycloalkyl, wherein said C5-C6 cycloalkyl is fused to a benzene ring;
and wherein RHC is unsubstituted or substituted with 1 to 2 R12 moieties independently selected from the group consisting of halo and —CN;


II) or R6a and R6b together with the N atom to which they are attached form R6H, wherein R6H is azetidinyl, pyrrolidinyl, piperidinyl, or piperazinyl;
wherein R6H is substituted with —Z—RCY, wherein
Z is a bond; and
RCY is unsubstituted phenyl or phenyl substituted by 1 to 2 R10 moieties selected from the group consisting of halo and —CN;

R6H is optionally substituted by 1 to 2 R9 moieties, wherein each R9 moiety is independently C1-C3 alkyl, halo or —CN, and


R2 is unsubstituted or substituted by 1 to 2 R5 groups independently selected from the group consisting of fluoro, chloro, C1-C3 alkyl, trifluoromethyl, C1-C3 alkoxy, —CN, and —OCF3.

14. The compound of claim 1 or a pharmaceutically acceptable salt thereof, wherein the compound is selected from the group consisting of
5-(3-phenyl-7-{[4-(trifluoromethyl)benzyl]carbamoyl}pyrido[2,3-b]pyrazin-2-yl)pentanoic acid,
5-{7-[(4-chlorobenzyl)carbamoyl]-3-phenylpyrido[2,3-b]pyrazin-2-yl}pentanoic acid,
5-{3-phenyl-7-[(4-phenylpiperazin-1-yl)carbonyl]pyrido[2,3-b]pyrazin-2-yl}pentanoic acid,
5-(3-phenyl-7-{[(1R)-1-phenylethyl]carbamoyl}quinoxalin-2-yl)pentanoic acid,
5-(3-phenyl-7-{[(1S)-1-phenylethyl]carbamoyl}quinoxalin-2-yl)pentanoic acid,
5-(3-phenyl-7-{[4-(trifluoromethyl)benzyl]carbamoyl}quinoxalin-2-yl)pentanoic acid,
5-(7-{[(1R)-1-(4-fluorophenyl)ethyl]carbamoyl}-3-phenylquinoxalin-2-yl)pentanoic acid,
5-(7-{[(1S)-1-(4-fluorophenyl)ethyl]carbamoyl}-3-phenylquinoxalin-2-yl)pentanoic acid,
5-{7-[(3-benzylpiperazin-1-yl)carbonyl]-3-phenylquinoxalin-2-yl}pentanoic acid,
5-(7-{[3-(4-methoxyphenyl)pyrrolidin-1-yl]carbonyl}-3-phenylquinoxalin-2-yl)pentanoic acid,
5-(7-{[3-(3-chlorophenyl)pyrrolidin-1-yl]carbonyl}-3-phenylquinoxalin-2-yl)pentanoic acid,
5-(3-phenyl-7-{[2,2,2-trifluoro-1-(4-fluorophenyl)ethyl]carbamoyl}quinoxalin-2-yl)pentanoic acid,
5-{7-[(3-benzylpyrrolidin-1-yl)carbonyl]-3-phenylquinoxalin-2-yl}pentanoic acid,
5-(7-{[3-(4-fluorophenyl)pyrrolidin-1-yl]carbonyl}-3-phenylquinoxalin-2-yl)pentanoic acid,
5-{7-[(4-chlorobenzyl)carbamoyl]-3-phenylquinoxalin-2-yl}pentanoic acid,
5-{7-[(1-methyl-1-phenylethyl)carbamoyl]-3-phenylquinoxalin-2-yl}pentanoic acid,
5-[7-(benzylcarbamoyl)-3-phenylquinoxalin-2-yl]pentanoic acid,
5-{7-[(2-fluorobenzyl)carbamoyl]-3-phenylquinoxalin-2-yl}pentanoic acid,
5-{7-[(3-fluorobenzyl)carbamoyl]-3-phenylquinoxalin-2-yl}pentanoic acid,
5-{7-[(4-fluorobenzyl)carbamoyl]-3-phenylquinoxalin-2-yl}pentanoic acid,
5-{3-phenyl-7-[(pyridin-2-ylmethyl)carbamoyl]quinoxalin-2-yl}pentanoic acid,
5-{3-phenyl-7-[(4-phenylpiperidin-1-yl)carbonyl]quinoxalin-2-yl}pentanoic acid,
5-{3-phenyl-7-[(2-phenylpiperidin-1-yl)carbonyl]quinoxalin-2-yl}pentanoic acid,
5-{3-phenyl-7-[(3-phenylpyrrolidin-1-yl)carbonyl]quinoxalin-2-yl}pentanoic acid,
5-{3-phenyl-7-[(2-phenylpyrrolidin-1-yl)carbonyl]quinoxalin-2-yl}pentanoic acid,
5-{3-phenyl-7-[(4-phenylpiperazin-1-yl)carbonyl]quinoxalin-2-yl}pentanoic acid,
5-{7-[benzyl(methyl)carbamoyl]-3-phenylquinoxalin-2-yl}pentanoic acid,
5-[7-(3,4-dihydroisoquinolin-2(1H)-ylcarbonyl)-3-phenylquinoxalin-2-yl]pentanoic acid,
5-[3-phenyl-7-(1,2,3,4-tetrahydronaphthalen-1-ylcarbamoyl)quinoxalin-2-yl]pentanoic acid,
5-(7-{[(1-(4-methoxyphenyl)ethyl]carbamoyl}-3-phenylquinoxalin-2-yl)pentanoic acid,
5-(3-phenyl-7-{[4-(trifluoromethoxy)benzyl]carbamoyl}quinoxalin-2-yl)pentanoic acid,
5-{7-[(4-methylbenzyl)carbamoyl]-3-phenylquinoxalin-2-yl}pentanoic acid,
5-(7-{[(1R)-1-(4-chlorophenyl)ethyl]carbamoyl}-3-phenylquinoxalin-2-yl)pentanoic acid,
5-(7-{[(1R)-1-(4-methylphenyl)ethyl]carbamoyl}-3-phenylquinoxalin-2-yl)pentanoic acid,
5-{7-[(4-ethylbenzyl)carbamoyl]-3-phenylquinoxalin-2-yl}pentanoic acid,
5-{7-[(4-methoxybenzyl)carbamoyl]-3-phenylquinoxalin-2-yl}pentanoic acid,
5-{7-[(4-cyanobenzyl)carbamoyl]-3-phenylquinoxalin-2-yl}pentanoic acid,
5-(7-{[(1S)-1-(4-methylphenyl)ethyl]carbamoyl}-3-phenylquinoxalin-2-yl)pentanoic acid,
5-[3-phenyl-7-({1-[4-(trifluoromethoxy)phenyl]ethyl}carbamoyl)quinoxalin-2-yl]pentanoic acid,
5-[3-phenyl-7-(piperidin-1-ylcarbonyl)quinoxalin-2-yl]pentanoic acid,
5-(7-{[1-(2-fluorophenyl)ethyl]carbamoyl}-3-phenylquinoxalin-2-yl)pentanoic acid,
5-[7-(2,3-dihydro-1H-inden-2-ylcarbamoyl)-3-phenylquinoxalin-2-yl]pentanoic acid,
5-(7-{[(1S)-1-(4-chlorophenyl)ethyl]carbamoyl}-3-phenylquinoxalin-2-yl)pentanoic acid,
5-(7-{[3-(2-oxopyrrolidin-1-yl)propyl]carbamoyl}-3-phenylquinoxalin-2-yl)pentanoic acid,
5-(7-{[4-(4-methyl-1H-1,2,3-triazol-1-yl)piperidin-1-yl]carbonyl}-3-phenylquinoxalin-2-yl)pentanoic acid,
5-(7-{[4-(5-methyl-1H-1,2,3-triazol-1-yl)piperidin-1-yl]carbonyl}-3-phenylquinoxalin-2-yl)pentanoic acid,
5-{3-phenyl-7-[(4-pyrazin-2-ylpiperidin-1-yl)carbonyl]quinoxalin-2-yl}pentanoic acid,
5-(7-{[(4-chloropyridin-2-yl)methyl]carbamoyl}-3-phenylquinoxalin-2-yl)pentanoic acid,
5-(7-{[(6-chloropyridin-2-yl)methyl]carbamoyl}-3-phenylquinoxalin-2-yl)pentanoic acid,
3-(4-chlorophenyl)-7-[[[(4-chlorophenyl)methyl]amino]carbonyl]-2-quinoxalinepentanoic acid,
3-(4-chlorophenyl)-7-[[[(3-chlorophenyl)methyl]amino]carbonyl]-2-quinoxalinepentanoic acid,
3-(4-chlorophenyl)-7-[(3-phenyl-1-pyrrolidinyl)carbonyl]-2-quinoxalinepentanoic acid,
3-(4-chlorophenyl)-7-[[(2,2,2-trifluoro-1(S)-phenylethyl)amino]carbonyl]-2-quinoxalinepentanoic acid,
3-(4-chlorophenyl)-7-[(4-phenyl-1-piperidinyl)carbonyl]-2-quinoxalinepentanoic acid,
3-(4-chlorophenyl)-7-[[[(4-cyanophenyl)methyl]amino]carbonyl]-2-quinoxalinepentanoic acid,
3-(4-chlorophenyl)-7-[(3 (R)-phenyl-1-pyrrolidinyl)carbonyl]-2-quinoxalinepentanoic acid,
3-(4-chlorophenyl)-7-[(3 (R)-phenyl-1-piperidinyl)carbonyl]-2-quinoxalinepentanoic acid,
3-(4-chlorophenyl)-7-[[[[4-(trifluoromethoxy)phenyl]methyl]amino]carbonyl]-2-quinoxalinepentanoic acid,
3-(4-chlorophenyl)-7-[[[(4-fluorophenyl)methyl]amino]carbonyl]-2-quinoxalinepentanoic acid,
3-(4-chlorophenyl)-7-[[[1(R)-(4-fluorophenyl)ethyl]amino]carbonyl]-2-quinoxalinepentanoic acid,
3-(4-chlorophenyl)-7-[[[1(S)-(4-fluorophenyl)ethyl]amino]carbonyl]-2-quinoxalinepentanoic acid,
3-(4-chlorophenyl)-7-[[[[4-(trifluoromethyl)phenyl]methyl]amino]carbonyl]-2-quinoxalinepentanoic acid,
3-(4-chlorophenyl)-7-[[[1(R)-(4-chlorophenyl)-2,2,2-trifluoroethyl]amino]carbonyl]-2-quinoxalinepentanoic acid,
3-(4-chlorophenyl)-7-[[[2,2,2-trifluoro-1(S)-(4-fluorophenyl)ethyl]amino]carbonyl]-2-quinoxalinepentanoic acid,
3-(4-chlorophenyl)-7-[[[[3-(trifluoromethyl)phenyl]methyl]amino]carbonyl]-2-quinoxalinepentanoic acid,
3-(4-chlorophenyl)-7-[[[[3-(trifluoromethoxy)phenyl]methyl]-amino]carbonyl]-2-quinoxalinepentanoic acid,
3-(4-chlorophenyl)-7-[[[1(S)-(4-chlorophenyl)ethyl]amino]carbonyl]-2-quinoxalinepentanoic acid,
3-(4-chlorophenyl)-7-[(4-phenyl-1-piperazinyl)carbonyl]-2-quinoxalinepentanoic acid,
3-(4-chlorophenyl)-7-[[(2,2,2-trifluoro-1(R)-phenylethyl)amino]carbonyl]-2-quinoxalinepentanoic acid,
3-(4-chlorophenyl)-7-[(4-cyano-4-phenyl-1-piperidinyl)carbonyl]-2-quinoxalinepentanoic acid,
3-(4-chlorophenyl)-7-[[[(4-methylphenyl)methyl]amino]carbonyl]-2-quinoxalinepentanoic acid,
3-(4-chlorophenyl)-beta-methyl-7-[(4-phenyl-1-piperidinyl)carbonyl]-2-quinoxalinepentanoic acid,
3-(4-chlorophenyl)-7-[[[1(S)-(4-chlorophenyl)ethyl]amino]carbonyl]-beta-methyl-2-quinoxalinepentanoic acid,
3-(4-chlorophenyl)-beta-methyl-7-[[[2,2,2-trifluoro-1(S)-phenylethyl]amino]carbonyl]-2-quinoxalinepentanoic acid,
beta-methyl-3-[4-(trifluoromethyl)phenyl]-7-[[(2,2,2-trifluoro-1(S)-phenylethyl)amino]carbonyl]-2-quinoxalinepentanoic acid,
alpha-methyl-7-[(4-phenyl-1-piperidinyl)carbonyl]-3-[4-(trifluoromethyl)phenyl]-2-quinoxalinepentanoic acid,
3-[2-[3-(4-chlorophenyl)-7-[(4-phenyl-1-piperidinyl)carbonyl]-2-quinoxalinyl]ethyl]benzeneacetic acid,
3-[2-[3-(4-chlorophenyl)-7-[[[1(R)-(4-fluorophenyl)ethyl]amino]carbonyl]-2-quinoxalinyl]ethyl]benzeneacetic acid,
2-[2-[3-phenyl-7-[(4-phenyl-1-piperidinyl)carbonyl]-2-quinoxalinyl]ethyl]cyclopropanecarboxylic acid,
6-fluoro-3-phenyl-7-[(3(S)-phenyl-1-pyrrolidinyl)carbonyl]-2-quinoxalinepentanoic acid,
3-(3-fluorophenyl)-7-[(3-phenyl-1-pyrrolidinyl)carbonyl]-2-quinoxalinepentanoic acid,
3-(3-chlorophenyl)-7-[(4-phenyl-1-piperidinyl)carbonyl]-2-quinoxalinepentanoic acid,
7-[(4-phenyl-1-piperidinyl)carbonyl]-3-[6-(trifluoromethyl)-3-pyridinyl]-2-quinoxalinepentanoic acid,
3-(2-chlorophenyl)-7-[(4-phenyl-1-piperidinyl)carbonyl]-2-quinoxalinepentanoic acid,
7-[(4-phenyl-1-piperidinyl)carbonyl]-3-[4-(trifluoromethoxy)phenyl]-2-quinoxalinepentanoic acid,
3-(4-methoxyphenyl)-7-[(4-phenyl-1-piperidinyl)carbonyl]-2-quinoxalinepentanoic acid,
3-(4-cyanophenyl)-7-[(4-phenyl-1-piperidinyl)carbonyl]-2-quinoxalinepentanoic acid,
7-[(4-phenyl-1-piperidinyl)carbonyl]-3-(3-thienyl)-2-quinoxalinepentanoic acid,
3-(2,4-dichlorophenyl)-7-[(4-phenyl-1-piperidinyl)carbonyl]-2-quinoxalinepentanoic acid,
(R)-5-(7-(4-(4-fluorophenyl)-2-methylpiperazine-1-carbonyl)-3-phenylquinoxalin-2-yl)pentanoic acid,
(R)-5-(7-((6-fluoro-1,2,3,4-tetrahydronaphthalen-1-yl)carbamoyl)-3-phenylquinoxalin-2-yl)pentanoic acid,
(R)-5-(7-(2-methyl-4-phenylpiperazine-1-carbonyl)-3-phenylquinoxalin-2-yl)pentanoic acid,
4-((7-(4-(4-fluorophenyl)piperazine-1-carbonyl)-3-phenylquinoxalin-2-yl)amino)butanoic acid,
(R)-4-((3-phenyl-7-((1,2,3,4-tetrahydronaphthalen-1-yl)carbamoyl)quinoxalin-2-yl)amino)butanoic acid,
7-[[[(4-chlorophenyl)methyl]amino]carbonyl]-3-(4-fluorophenyl)-2-quinoxalinepentanoic acid,
3-(4-fluorophenyl)-7-[[(2,2,2-trifluoro-1(S)-phenylethyl)amino]carbonyl]-2-quinoxalinepentanoic acid,
7-[[(2,3-dihydro-1H-inden-2-yl)amino]carbonyl]-3-(4-fluorophenyl)-2-quinoxalinepentanoic acid,
7-[[[(4-cyanophenyl)methyl]amino]carbonyl]-3-(4-fluorophenyl)-2-quinoxalinepentanoic acid,
3-(4-fluorophenyl)-7-[(4-phenyl-1-piperidinyl)carbonyl]-2-quinoxalinepentanoic acid,
3-(4-fluorophenyl)-7-[(3-phenyl-1-pyrrolidinyl)carbonyl]-2-quinoxalinepentanoic acid,
3-(4-fluorophenyl)-7-[[[[4-(trifluoromethoxy)phenyl]methyl]amino]carbonyl]-2-quinoxalinepentanoic acid,
3-(4-fluorophenyl)-7-[[[(4-fluorophenyl)methyl]amino]carbonyl]-2-quinoxalinepentanoic acid,
3-(4-fluorophenyl)-7-[[[1(R)-(4-fluorophenyl)ethyl]amino]carbonyl]-2-quinoxalinepentanoic acid,
3-(4-fluorophenyl)-7-[[[1(S)-(4-fluorophenyl)ethyl]amino]carbonyl]-2-quinoxalinepentanoic acid,
3-(4-fluorophenyl)-7-[[[[4-(trifluoromethyl)phenyl]methyl]amino]carbonyl]-2-quinoxalinepentanoic acid,
7-[[4-[2,3-dihydro-3-(2-methoxyethyl)-2-oxo-1H-benzimidazol-1-yl]-1-piperidinyl]carbonyl]-3-phenyl-2-quinoxalinepentanoic acid,
7-[[4-(2,3-dihydro-2-oxo-1H-benzimidazol-1-yl)-1-piperidinyl]carbonyl]-3-phenyl-2-quinoxalinepentanoic acid,
7-[(1,2-dihydro-1-methyl-2-oxospiro[3H-indole-3,4′-piperidin]-1′-yl)carbonyl]-3-phenyl-2-quinoxalinepentanoic acid,
3-phenyl-7-(spiro[benzofuran-3(2H),4′-piperidin]-1′-ylcarbonyl)-2-quinoxalinepentanoic acid,
7-[(2,3-dihydrospiro[1H-indene-1,4′-piperidin]-1′-yl)carbonyl]-3-phenyl-2-quinoxalinepentanoic acid,
7-[(1,2-dihydro-1-methylspiro[3H-indole-3,4′-piperidin]-1′-yl)carbonyl]-3-phenyl-2-quinoxalinepentanoic acid,
7-[(1-acetyl-1,2-dihydrospiro[3H-indole-3,4′-piperidin]-1′-yl)carbonyl]-3-phenyl-2-quinoxalinepentanoic acid,
7-[(4-cyano-4-phenyl-1-piperidinyl)carbonyl]-3-phenyl-2-quinoxalinepentanoic acid,
7-[(4-oxo-1-phenyl-1,3,8-triazaspiro[4.5]dec-8-yl)carbonyl]-3-phenyl-2-quinoxalinepentanoic acid,
3-(4-fluorophenyl)-7-[(4-oxo-1-phenyl-1,3,8-triazaspiro[4.5]dec-8-yl)carbonyl]-2-quinoxalinepentanoic acid,
5-(3-(4-fluorophenyl)-7-(4-phenyl-1,2,3,6-tetrahydropyridine-1-carbonyl)quinoxalin-2-yl)pentanoic acid,
7-[(4-cyano-4-phenyl-1-piperidinyl)carbonyl]-3-(4-fluorophenyl)-2-quinoxalinepentanoic acid,
3-(4-fluorophenyl)-7-[[(phenylmethyl)amino]carbonyl]-2-quinoxalinepentanoic acid,
7-[[[1(R)-(4-chlorophenyl)ethyl]amino]carbonyl]-3-(4-fluorophenyl)-2-quinoxalinepentanoic acid,
7-[[[1(S)-(4-chlorophenyl)ethyl]amino]carbonyl]-3-(4-fluorophenyl)-2-quinoxalinepentanoic acid,
3-(4-fluorophenyl)-7-[[(1(R)-phenylethyl)amino]carbonyl]-2-quinoxalinepentanoic acid,
7-[[4-(4-chlorophenyl)-1-piperidinyl]carbonyl]-3-(4-fluorophenyl)-2-quinoxalinepentanoic acid,
7-[[4-(4-chlorophenyl)-3,6-dihydro-1(2H)-pyridinyl]carbonyl]-3-(4-fluorophenyl)-2-quinoxalinepentanoic acid,
3-phenyl-7-[(3(S)-phenyl-1-piperidinyl)carbonyl]-2-quinoxalinepentanoic acid,
3-phenyl-7-[(3(R)-phenyl-1-piperidinyl)carbonyl]-2-quinoxalinepentanoic acid,
5-(7-(4-(1H-imidazol-1-yl)piperidine-1-carbonyl)-3-phenylquinoxalin-2-yl)pentanoic acid,
7-[[[1(R)-(2-pyridinyl)ethyl]amino]carbonyl]-3-[4-(trifluoromethyl)phenyl]-2-quinoxalinepentanoic acid,
7-[(4-phenyl-1-piperidinyl)carbonyl]-3-[4-(trifluoromethyl)phenyl]-2-quinoxalinepentanoic acid,
3-(4-chlorophenyl)-alpha-methyl-7-[(4-phenyl-1-piperidinyl)carbonyl]-2-quinoxalinepentanoic acid,
3-(4-chlorophenyl)-alpha-methyl-7-[[(2,2,2-trifluoro-1(S)-phenylethyl)amino]carbonyl]-2-quinoxalinepentanoic acid,
3-(4-chlorophenyl)-7-[[[(4-cyanophenyl)methyl]amino]carbonyl]-alpha-methyl-2-quinoxalinepentanoic acid,
3-(4-chlorophenyl)-7-[[[1(R)-(4-fluorophenyl)ethyl]amino]carbonyl]-alpha-methyl-2-quinoxalinepentanoic acid,
3-(4-chlorophenyl)-7-[[[1(S)-(4-fluorophenyl)ethyl]amino]carbonyl]-alpha-methyl-2-quinoxalinepentanoic acid,
3-phenyl-7-[(3-phenyl-1-azetidinyl)carbonyl]-2-quinoxalinepentanoic acid,
7-[[3-(4-cyanophenyl)-1-azetidinyl]carbonyl]-3-phenyl-2-quinoxalinepentanoic acid,
3-(4-fluorophenyl)-7-[[(1,2,3,4-tetrahydro-1(R)-naphthalenyl)amino]carbonyl]-2-quinoxalinepentanoic acid,
3-(4-fluorophenyl)-7-[(4-phenyl-1-piperazinyl)carbonyl]-2-quinoxalinepentanoic acid,
7-[[3-(3-fluorophenyl)-1-azetidinyl]carbonyl]-3-phenyl-2-quinoxalinepentanoic acid,
7-[[(6-fluoro-1,2,3,4-tetrahydro-1(R)-naphthalenyl)amino]carbonyl]-3-phenyl-2-quinoxalinepentanoic acid,
(R)-5-(3-phenyl-7-((1,2,3,4-tetrahydronaphthalen-1-yl)carbamoyl)quinoxalin-2-yl)pentanoic acid,
(S)-5-(3-(4-chlorophenyl)-7-((1-(3,4-difluorophenyl)ethyl)carbamoyl)quinoxalin-2-yl)pentanoic acid,
(R)-5-(3-(4-chlorophenyl)-7-((1-(3,5-difluorophenyl)ethyl)carbamoyl)quinoxalin-2-yl)pentanoic acid,
5-(3-(4-chlorophenyl)-7-(3-(4-chlorophenyl)azetidine-1-carbonyl)quinoxalin-2-yl)pentanoic acid,
5-(7-(((R)-5-fluoro-2,3-dihydro-1H-inden-1-yl)carbamoyl)-3-(4-fluorophenyl)quinoxalin-2-yl)-2-methylpentanoic acid,
(R)-5-(7-(chroman-4-ylcarbamoyl)-3-(4-fluorophenyl)quinoxalin-2-yl)pentanoic acid,
(S)-5-(3-(4-chlorophenyl)-7-(chroman-4-ylcarbamoyl)quinoxalin-2-yl)pentanoic acid,
(R)-5-(7-(chroman-4-ylcarbamoyl)-3-phenylquinoxalin-2-yl)pentanoic acid,
5-(3-(4-chlorophenyl)-7-(3-oxo-3,4-dihydro-2H-spiro[isoquinoline-1,4′-piperidin]-1′-ylcarbonyl)quinoxalin-2-yl)pentanoic acid, and
5-(3-(4-chlorophenyl)-7-(4-(2,6-dimethylphenyl)piperazine-1-carbonyl)quinoxalin-2-yl)pentanoic acid.

15. A pharmaceutical composition comprising an effective amount of the compound of claim 1, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier.

16. A method for treating asthma, congestion, allergic rhinitis or COPD comprising administering to a patient in need of such treatment of an effective amount of a compound of claim 1 or a pharmaceutically acceptable salt thereof.

17. The compound of claim 1 or a pharmaceutically acceptable salt thereof, wherein R2 is phenyl, pyridyl, or thienyl; wherein R2 is unsubstituted or substituted by 1 to 2 R5 groups independently selected from the group consisting of fluoro, chloro, trifluoromethyl, C1-C3 alkoxy, —CN and —OCF3.

18. The compound of claim 13 or a pharmaceutically acceptable salt thereof, wherein
R1 is —C(O)—N(R6a)(R6b)
I) R6a is H and R6b is
a) -Q-RAH, wherein RAH is phenyl,
and wherein RAH is unsubstituted or substituted with 1 R8 moiety selected from the group consisting of fluoro and —CN;

b) -Q-RHC, wherein RHC is C5-C6 cycloalkyl, wherein said C5-C6 cycloalkyl is fused to a benzene ring;
and wherein RHC is unsubstituted or substituted with 1 to 2 R12 moieties independently selected from the group consisting of halo and —CN;


II) or R6a and R6b together with the N atom to which they are attached form R6H, wherein R6H is pyrrolidinyl, piperidinyl, or piperazinyl;
wherein R6H is substituted with —Z—RCY wherein
RCY is unsubstituted phenyl or phenyl substituted by 1 to 2 R10 moieties selected from the group consisting of halo and —CN;

R6H is optionally substituted by 1 to 2 R9 moieties wherein each R9 moiety is independently C1-C3 alkyl, halo or —CN,


R2 is unsubstituted phenyl; and
Q and Z are as set forth in claim 13.

19. The compound of claim 18 or a pharmaceutically acceptable salt thereof, wherein R6a and R6b together with the N atom to which they are attached form R6H, wherein R6H is piperazinyl.

20. The compound of claim 13 or a pharmaceutically acceptable salt thereof, wherein the compound is selected from the group consisting of
(R)-4-((7-(4-(4-fluorophenyl)-2-methylpiperazine-1-carbonyl)-3-phenylquinoxalin-2-yl)amino)butanoic acid,
(R)-4-((7-((1-(4-fluorophenyl)ethyl)carbamoyl)-3-phenylquinoxalin-2-yl)amino)butanoic acid,
(R)-4-((7-((6-fluoro-1,2,3,4-tetrahydronaphthalen-1-yl)carbamoyl)-3-phenylquinoxalin-2-yl)amino)butanoic acid,
4-((3-phenyl-7-(4-phenylpiperazine-1-carbonyl)quinoxalin-2-yl)amino)butanoic acid,
(R)-4-((7-((1-(4-cyanophenyl)ethyl)carbamoyl)-3-phenylquinoxalin-2-yl)amino)butanoic acid,
4-((7-(4-(4-fluorophenyl)piperazine-1-carbonyl)-3-phenylquinoxalin-2-yl)amino)butanoic acid,
(R)-4-((3-phenyl-7-((1,2,3,4-tetrahydronaphthalen-1-yl)carbamoyl)quinoxalin-2-yl)amino)butanoic acid,
4-((7-(4-(4-fluorophenyl)piperidine-1-carbonyl)-3-phenylquinoxalin-2-yl)amino)butanoic acid,
4-((7-(4-(4-chlorophenyl)piperidine-1-carbonyl)-3-phenylquinoxalin-2-yl)amino)butanoic acid,
4-((7-(4-(4-chlorophenyl)piperazine-1-carbonyl)-3-phenylquinoxalin-2-yl)amino)butanoic acid,
(R)-4-((3-phenyl-7-(3-phenylpyrrolidine-1-carbonyl)quinoxalin-2-yl)amino)butanoic acid, and
(R)-4-((7-(2-methyl-4-phenylpiperazine-1-carbonyl)-3-phenylquinoxalin-2-yl)amino)butanoic acid.

21. The compound of claim 13 or a pharmaceutically acceptable salt thereof, wherein the compound is (R)-4-((7-((6-fluoro-1,2,3,4-tetrahydronaphthalen-1-yl)carbamoyl)-3-phenylquinoxalin-2-yl)amino)butanoic acid.

22. The compound of claim 13 or a pharmaceutically acceptable salt thereof, wherein the compound is 4-((3-phenyl-7-(4-phenylpiperazine-1-carbonyl)quinoxalin-2-yl)amino)butanoic acid.

23. The compound of claim 13 or a pharmaceutically acceptable salt thereof, wherein the compound is (R)-4-((7-(4-(4-fluorophenyl)-2-methylpiperazine-1-carbonyl)-3-phenylquinoxalin-2-yl)amino)butanoic acid.

24. The compound of claim 13 or a pharmaceutically acceptable salt thereof, wherein the compound is (R)-4-((7-((1-(4-cyanophenyl)ethyl)carbamoyl)-3-phenylquinoxalin-2-yl)amino)butanoic acid.

25. The compound of claim 13 or a pharmaceutically acceptable salt thereof, wherein the compound is 4-((7-(4-(4-fluorophenyl)piperazine-1-carbonyl)-3-phenylquinoxalin-2-yl)amino)butanoic acid.

26. The compound of claim 13 or a pharmaceutically acceptable salt thereof, wherein the compound is (R)-4-((3-phenyl-7-((1,2,3,4-tetrahydronaphthalen-1-yl)carbamoyl)quinoxalin-2-yl)amino)butanoic acid.

27. The method of claim 16, wherein the method is for treating asthma in a patient in need of such treatment.