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                            Library & Multimedia
 Raman spectroscopic signature of semen and its potential application to forensic body fluid identification
NCJ Number
              307001
Journal

*Forensic Science International* Volume: 193 Issue: 1-3 Dated: 2009 Pages: 56-62
Author(s)
          Kelly Virkler;
                        Igor K. Lednev
Date Published

December 2009
Length
              7 pages
Annotation
              This article presents research into the role of heterogeneity, as well as among multiple donors, for body fluid samples found at a crime scene.
Abstract
              A great potential of Raman spectroscopy for non-destructive, confirmatory identification of body fluids at the crime scene has been reported recently (Virkler and Lednev, Forensic Sci. Int. 2008 [5]). However, that analysis was carried out on only one sample of each body fluid and did not take into account any variations that might occur between different donors of the same fluid. This paper reports on the role of heterogeneity within a sample as well as among multiple donors for human semen. Near-infrared (NIR) Raman spectroscopy was used to measure spectra of pure dried human semen samples from multiple donors in a controlled laboratory environment. The major chemical components that contributed to the Raman spectrum of semen were determined and used to tentatively identify the principal spectral components. The issue of potential spectral variations that could arise between different donors of semen was also addressed. Advanced statistical analysis of spectra obtained from multiple spots on dry samples showed that dry semen is heterogeneous and its Raman spectra could be presented as a linear combination of a fluorescent background and three spectral components. The relative contribution of each of the three components varies with donor, so no single spectrum could effectively represent an experimental Raman spectrum of dry semen in a quantitative way. The combination of the three spectral components could be considered to be a spectroscopic signature for semen. This proof-of-concept approach shows the potential for Raman spectroscopy to identify an unknown substance to be semen during forensic analysis.(Published Abstract Provided)
      
  
          Additional Details
Grant Number(s)
        2009-DN-BX-K186


  
  
        
  Agencies
        NIJ-Sponsored


  
  
        
  Publication Type
        Research (Applied/Empirical)


  
  
        
  Award
        Application of Raman Spectroscopy for an Easy-to-Use, on-Field, Rapid, Nondestructive, Confirmatory Identification of Body Fluids
    
  
  
Date Published: December 1, 2009
    
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