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                            Library & Multimedia
 Validation of Half-Reaction Amplification Using Promega PowerPlex© 16
NCJ Number
              304246
Journal

*Journal of Forensic Sciences* Volume: 55 Issue: 4 Dated: 2010 Pages: 1044-1049
Author(s)
          Nicole H. Hoffman M.S.;
                        Terry Fenger Ph.D.
Date Published

2010
Length
              6 pages
Annotation
              Since DNA amplification is a fundamental, yet costly process used in DNA analysis, this study evaluated half-reaction amplification (12.5, 12, and 13 uL), using the Promega Powerplex® 16 Kit, hoping to reduce sample analysis costs by half.
Abstract
              A sensitivity study was completed, along with the testing of various blood-stain samples including those with low (<0.40 ng) and high DNA concentrations (>3.0 ng), peak height imbalances, and allelic drop-out. Also, 467 samples submitted to the MUFSC laboratory for testing were analyzed. Results indicate that half-reaction amplification produced higher quality profiles than full-reactions. Average peak heights increased by 85 percent, peak height imbalances improved, and drop-out was eliminated in 75.8 percent of samples. Only eight of 467 case samples required re-amplification, a success rate of 94 percent was observed, and the repeat rate decreased significantly. Finally, a DNA input of 0.25–1.0 ng is ideal for half-reaction amplification. (publisher abstract modified)
      
  
          Additional Details
Grant Number(s)
        2005-MU-BX-K020


  
  
        
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        NIJ-Sponsored


  
  
        
  Award
        MUFSC and FRN Initiatives to Improve Forensic Crime Labs
    
  
  
Date Published: January 1, 2010
    
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        DNA (Deoxyribonucleic Acid)



  
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