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                            Library & Multimedia
 Utility of the Ion S5 (TM) and MiSeq FGx (TM) sequencing platforms to characterize challenging human remains
NCJ Number
              301737
Journal

*Legal Medicine* Volume: 41 Dated: 2019
Author(s)
          K. Elwick;
                        et al
Date Published

2019
Annotation
              In the study reported in this article, bone and tooth samples exposed to a variety of DNA insults (cremation, embalming, decomposition, thermal degradation, and fire) were assessed and sequenced using the Precision ID chemistry and a custom AmpliSeqTM STR and iiSNP panel on the Ion S5TM System, and the ForenSeq DNA Signature Prep Kit on the MiSeq FGxTM system, as well as the GlobalFilerTM PCR Amplification Kit on the 3500TM Genetic Analyzer.
Abstract
              Often in missing persons’ and mass disaster cases, the samples remaining for analysis are hard tissues such as bones, teeth, nails, and hair. These remains may have been exposed to harsh environmental conditions, which pose challenges for downstream genotyping. Short tandem repeat analysis (STR) via capillary electrophoresis (CE) is still the gold standard for DNA typing; however, a newer technology known as massively parallel sequencing (MPS) could improve current techniques by typing different and more markers in a single analysis, consequently improving the power of discrimination. The results of the current study demonstrated that using traditional CE-based genotyping performed as expected, producing a partial or full DNA profile for all samples, and that both sequencing chemistries and platforms were able to recover sufficient STR and SNP information from a majority of the same challenging samples. Run metrics, including profile completeness and mean read depth, produced good results with each system, considering the degree of damage of some samples. Most sample insults (except decomposed) produced similar numbers of alleles for both MPS systems. Comparable markers produced full concordance between the two platforms. (publisher abstract modified)
      
  
          Additional Details
Grant Number(s)
        2015-DN-BX-K066


  
  
        
  Agencies
        NIJ-Sponsored


  
  
        
  Award
        Research and Development in Forensic Science for Criminal Justice Purposes (DVI)
    
  
  
Date Published: January 1, 2019
    
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