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 Animation that explains more about how a profile is derived from an electropherogram

August 5, 2020
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0:00 Shown above is a single-source electropherogram

0:03 that is the electropherogram of a sample that  is known to represent only one person's DNA.

0:12 In order to help the analyst more easily derive  useful information from the electropherogram,

0:17 profiling software determines the  number of STRs represented by each peak

0:22 and labels them all accordingly. Notice that  the red peaks are disregarded during profiling

0:27 as they merely represent the size  standard used during electrophoresis

0:32 the resulting chart of labeled peaks  is the sample contributors STR profile.

0:40 When a sample or samples are  provided to an analyzing lab,

0:44 the lab will deliver a report on the profile of  each contributor found in the samples. This report

0:50 will list the alleles in a table labeled according  to the number of STRs each has and categorized

0:56 according to contributor and the dye with which  they were identified, which indicates the locus.

1:02 Any one sample may require more than one series  of tests in order to identify all of its alleles

1:09 Notice that the table is already partially  filled in from a previous series of tests

1:13 that were performed on this sample. Click  next to view all three charts compare the

1:20 electropherogram that is produced by the initial analysis to the corresponding profile to the

1:26 final results table that presents the profile  data from the testing of all 13 core loci.

1:35 Move the mouse cursor over the profile labels to

1:37 see where those alleles are  listed on the results table.
    
  
Disclaimer:
              Opinions or points of view expressed in these recordings represent those of the speakers and do not necessarily represent the official position or policies of the U.S. Department of Justice. Any commercial products and manufacturers discussed in these recordings are presented for informational purposes only and do not constitute product approval or endorsement by the U.S. Department of Justice.
  
Date Published: August 5, 2020
    
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