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                            Library & Multimedia
 A Novel, Integrated Forensic Microdevice on a Rotation-Driven Platform - Buccal Swab to STR Product in Less than Two Hours
NCJ Number
              252087
Journal

*Electrophoresis* Volume: 37 Issue: 23-24 Dated: 2016 Pages: 3046-3058
Author(s)
          Jordan O. Cox;
                        Teresa S. DeCarmen;
                        Yiwen Ouyang;
                        Briony Strachan;
                        Hillary Sloane;
                        Cathey Connon;
                        Kemper Gibson;
                        Kimberly Jackson;
                        James P. Landers;
                        Tracey Dawson Cruz
Date Published

2016
Length
              13 pages
Annotation
              This article describes the development of a novel microdevice for forensic DNA processing of reference swabs.
Abstract
              This microdevice incorporates an enzyme-based assay for DNA preparation, which allows for faster processing times and reduced sample handling. Infrared-mediated PCR (IR-PCR) is used for STR amplification using a custom reaction mixture, allowing for amplification of STR loci in 45 min while circumventing the limitations of traditional block thermocyclers. Uniquely positioned valves coupled with a simple rotational platform are used to exert fluidic control, eliminating the need for bulky external equipment. All microdevices were fabricated using laser ablation and thermal bonding of PMMA layers. Using this microdevice, the enzyme-mediated DNA liberation module produced DNA yields similar to or higher than those produced using the traditional (tube-based) protocol. Initial microdevice IR-PCR experiments to test the amplification module and reaction (using Phusion Flash/SpeedSTAR) generated near-full profiles that suffered from interlocus peak imbalance and poor adenylation (significant -A); however, subsequent attempts using KAPA 2G and Pfu Ultra polymerases generated full STR profiles with improved interlocus balance and the expected adenylated product. A fully integrated run designed to test microfluidic control successfully generated CE-ready STR amplicons in less than 2 h (<1 h of hands-on time). Using this approach, high-quality STR profiles were developed that were consistent with those produced using conventional DNA purification and STR amplification methods. This method is a smaller, more elegant solution than current microdevice methods and offers a cheaper, hands-free, closed-system alternative to traditional forensic methods. (Publisher abstract modified)
      
  
          Additional Details
Grant Number(s)
        2016-NE-BX-0002


  
  
        
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        NIJ-Sponsored


  
  
        
  Publication Type
        Research (Applied/Empirical)
                                Report (Study/Research)
                                Report (Grant Sponsored)
                                Program/Project Description


  
  
        
  Award
        A rotational platform-driven microdevice for differential separation, purification, & amplification of sexual assault forensic samples
    
  
  
Date Published: January 1, 2016
    
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