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                            Library & Multimedia
 A simple multiplex polymerase chain reaction to determine ABO blood types of rhesus macaques (Macaca mulatta)
NCJ Number
              308328
Journal

*Tissue Antigens* Volume: 77 Issue: 6 Dated: 2011 Pages: 584-588
Author(s)
          A. Premasuthan;
                        S. Kanthaswamy;
                        J. Satkoski;
                        D. G. Smith
Date Published

2011
Length
              5 pages
Annotation
              In this paper, the authors describe an easy, rapid multiplex polymerase chain reaction to determine the ABO blood group phenotypes of rhesus macaques that can be performed with only small amounts of DNA.
Abstract
              Rhesus macaques are the most common nonhuman primate model organism used in biomedical research. Their increasingly frequent use as subjects in studies involving transplantation requires that blood and other tissue antigens of donors and recipients be compatible. In this paper, the authors report an easy and rapid multiplex polymerase chain reaction (PCR) to determine the ABO blood group phenotypes of rhesus macaques that can be performed with only small amounts of DNA. They phenotyped 78 individuals and found this species to exhibit the A, B, and AB phenotypes in frequencies that vary by geographic region. The probability of randomly pairing rhesus macaque donors and recipients that exhibit major ABO phenotype incompatibility is approximately 0.35 and 0.45 for Indian and Chinese rhesus macaques, respectively. (Published Abstracts Provided)
      
  
          Additional Details
Grant Number(s)
        2008-DN-BX-K288


  
  
        
  Agencies
        NIJ-Sponsored


  
  
        
  Publication Type
        Research (Applied/Empirical)


  
  
        
  Award
        Development of a DNA-Based Multi-Species Identification and Quantification Assay
    
  
  
Date Published: January 1, 2011
    
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