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                            Library & Multimedia
 Time Radically Alters Ex Situ Evidentiary Soil 16S Bacterial Profiles Produced Via Next-Generation Sequencing
NCJ Number
              253127
Journal

*Journal of Forensic Sciences* Volume: 63 Issue: 5 Dated: February 2018 Pages: 1356-1365
Author(s)
          Allyssa J. Badgley;
                        Ellen M. Jesmok;
                        David R. Foran
Date Published

February 2018
Length
              10 pages
Annotation
              This study placed soils collected from multiple habitats on evidence items that were samples over time, and then bacterial profiles were generated via next generation sequencing of the 16S rRNA locus.
Abstract
              Previous research has revealed the potential of soil bacterial profiling for forensic purposes; however, investigators have not thoroughly examined fluctuations in microbial profiles from soil aged on evidence. In the current study, bacterial abundance charts and nonmetric multidimensional scaling plots provided visual representation of bacterial profiles temporally, while supervised classification was used to statistically associate evidence to a source. The ex situ evidence soils displayed specific, consistent taxonomic changes as they aged, resulting in their drift in multidimensional space, but never toward a different habitat. Ninety five percent of the 364 evidentiary profiles statistically classified to the correct habitat, with misclassification generally stemming from evidence type and increased age. Ultimately, understanding bacterial changes that occur temporally in ex situ soils should enhance their use in forensic investigations. (publisher abstract modified)
      
  
          Additional Details
Grant Number(s)
        2013-R2-CX-K010
                                2015-DN-BX-K031


  
  
        
  Agencies
        NIJ-Sponsored


  
  
        
  Publication Type
        Research (Applied/Empirical)
                                Report (Study/Research)
                                Report (Grant Sponsored)
                                Program/Project Description


  
  
        
  Award
        Developing Reliable Methods for Microbial Fingerprinting of Soils
                                Developing Reliable Methods for Microbial Fingerprinting of Soil Evidence: Collection, Contamination, Storage, and Analysis
    
  
  
Date Published: February 1, 2018
    
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