U.S. flag
      An official website of the United States government, Department of Justice.
        Here's how you know
      Here's how you know**Official websites use .gov**
            
            A **.gov** website belongs to an official government organization in the United States.
      **Secure .gov websites use HTTPS**
            
            A **lock** (
            A locked padlock
            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites. Menu

      
        close

            
            
                                              About NIJ
        
                    
  
          About NIJ
      
    
          The NIJ Director
          How NIJ Is Organized
          Mission and Guiding Principles
          Research and Development Process
          Strategic Framework
          Assessments of NIJ
          Annual Reports & Reports to Congress
          Staff Directory
          Funding & Awards
        
                    
  
          Funding & Awards
      
    
          Current Funding
          Forthcoming Funding
          Expired Funding
          Awards: Listing of Funded Projects
          Guidance for Applicants and Awardees
          Fellowship & Student Programs
          Travel Scholarships
          NIJ Challenge Program
          Library & Multimedia
        
                    
  
          Library & Multimedia
      
    
          NIJ Journal
          Podcasts
          Publications Listing
          Multimedia Listing
          Rated Practices on CrimeSolutions
          Rated Programs on CrimeSolutions
          Software & Databases
          Datasets
          Patents
          Topics
        
                    
  
          Topics
      
    
          Article Listing
          Corrections
          Courts
          Crime Prevention
          Crimes
          Drugs and Crime
          Equipment and Technology
          Forensic Sciences
          Youth Justice
          Justice System Reform
          Law Enforcement
          Tribal Crime and Justice
          Victims of Crime
          Events
        
                    
  
          Events
      
    
          NIJ 2024 Research Conference
          Scheduled Events
          On-Demand Events
          Research Meetings & Workshops
          NIJ-Hosted Online Training

                            
                                About
          Contact Us
          Subscribe
          Topics A-Z
                  Share
FacebookXLinkedInRedditPinterestEmail Home
                            Library & Multimedia
 Twenty-Six plex Autosomal STR Assay To Aid Human Identity Testing
NCJ Number
              228503
Journal

*Journal of Forensic Sciences* Volume: 54 Issue: 5 Dated: September 2009 Pages: 1008-1015
Author(s)
          Carolyn R. Hill, M.S.;
                        John M. Butler, Ph.D.;
                        Peter M. Vallone, Ph.D.
Date Published

September 2009
Length
              8 pages
Annotation
              This report describes how to construct a large short tandem repeat (STR) multiplex so that others can understand the technology as well as how to resolve difficulties encountered throughout the process.
Abstract
              Researchers developed a single amplification 5-dye multiplex that contains 25 unlinked autosomal loci plus the sex-typing locus AMEL. The primary purpose of developing this multiplex is for use with reference samples in a variety of situations, such as paternity testing/kinship analysis, immigration testing, and for use in missing persons/mass disaster cases. Primers for a majority of the 25 autosomal STR loci were redesigned from the miniSTR loci in previous reports; they have already been determined to be unlinked from the 13 CODIS (Combined DNA Index System), as well as to be diverse with moderate-to-high heterozygosity values. They were comparable with the CODIS loci regarding heterozygosities with slightly lower mutation rates. This suggests that this STR multiplex will be a useful complement to the currently available commercial STR typing kits for the forensic analysis of DNA samples. Multiple strategies were used to overcome various challenges that arose while building this multiplex, such as incomplete adenylation, nonspecific products, artifacts, and simple primer incompatibility. To date, this multiplex represents the most STR loci typed together in a single PCR amplification assay. The development of the design process and optimization of the 26plex led to the identification of useful strategies for developing future large multiplexes. The descriptions of materials and methods address the multiplex design, multiplex design software, PCR amplification, analysis on the ABI 3130xl Genetic Analyzer, and sensitivity study. 2 tables, 5 figures, and 24 references
      
  
          Additional Details
Grant Number(s)
        2003-IJ-R-029


  
  
        
  Agencies
        NIJ-Sponsored


  
  
        
  Publication Type
        Report (Study/Research)
    
  
  
Date Published: September 1, 2009
    
          Downloads
        
                      HTML

Side navigation
  

        

  
  
              NIJ Journal
    
      
            
            
            
      Podcasts
    
      
            
            
            
      Publications Listing
    
      
            
            
            
      Multimedia Listing
    
      
            
            
            
      Rated Practices on CrimeSolutions
    
      
            
            
            
      Rated Programs on CrimeSolutions
    
      
            
            
            
      Software & Databases
    
      
            
            
            
      Datasets
    
      
            
            
            
      PatentsRelated Topics
        
                Criminology
        DNA fingerprinting
        Forensic sciences
        Investigative techniques
        NIJ Grant-related Documents



  
Similar Publications
        
                      Interactive Map: Medicolegal Death Investigation Data Flow
                  Understanding Work-Related Stress among Medicolegal Death Investigators: A National Survey and Mixed-Methods Impact Study
                  Fast On-site Screening of Seized Drugs by Electrochemical and Spectroscopic Tools: Identification of Fentanyl and Novel Psychoactive Substances
 Top

      National Institute of Justice

        
        
                        About NIJ
                    About NIJ
      
    
                  How NIJ Is Organized
      
    
                  NIJ Director
      
    
                  Research Disclaimer
      
    
                  Funding & Awards
                    Funding & Awards Home
      
    
                  Awards Listing
      
    
                  Current Funding
      
    
                  Guidance for Applicants and Awardees
      
    
                  Resources
                    Events
      
    
                  Podcasts
      
    
                  Publications
      
    
                  Topics
      
    
                  Multimedia
      
    
                  Reuse Policy
      
    
                  Contacts
                    Contact NIJ
      
    
                  Staff Directory
      
    
                  Submit a FOIA Request
      
    
                  File a Civil Rights ComplaintFacebook
          
                      X
          
                      YouTube
  

      810 7th St. NW, Washington, D.C. 20531
      
              Secondary Footer link menu
            Accessibility
              Plain Language
              Privacy Policy
              Legal Policies and Disclaimer
              No FEAR Act
              Freedom of Information Act
              USA.gov
              Justice.gov