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                            Library & Multimedia
 Variability and additivity of read counts for aSTRs in NGS DNA profiles
NCJ Number
              301563
Journal

*Forensic Science International-Genetics* Volume: 48 Dated: September 2020
Author(s)
          Kevin Cheng;
                        Jessica Skillman;
                        Stephanie Hickey;
                        Rebecca Just;
                        Lilliana Moreno;
                        Jo-Anne Bright;
                        Hannah Kelly;
                        Meng-Han Lin;
                        James M. Curran;
                        John Buckleton
Date Published

September 2020
Annotation
              This study examined next-generation sequencing (NGS) analyte signal variation, specifically heterozygous balance and stutter variability from profiles generated using the ForenSeqTM DNA Signature Prep Kit, DNA Primer Mix B.
Abstract
              There has been an increase in the number of laboratories and researchers adopting new sequencing technologies, known as next-generation sequencing (NGS). An understanding of the behavior of NGS DNA profiles is needed to enable the development of probabilistic genotyping methods for the interpretation of such profiles. The current study also investigated additivity of analyte signals in NGS profiles for overlapping allelic and stutter signals originating from the same or different contributors. Models are described that can be used to inform a continuous method for the interpretation of DNA profiling data. (publisher abstract modified)
      
  
          Additional Details
Grant Number(s)
        2017-DN-BX-0136


  
  
        
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        NIJ-Sponsored


  
  
        
  Publication Type
        Research (Applied/Empirical)


  
  
        
  Award
        Population Genetic Issues for Forensic DNA Profiles
    
  
  
Date Published: September 1, 2020
    
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