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                            Library & Multimedia
 Technical note: Survey of extracellular and cell-pellet-associated DNA from 'touch'/trace samples
NCJ Number
              304562
Journal

*Forensic Science International* Volume: 318 Dated: January 2021
Author(s)
          Mekhi Miller;
                        M. Katherine Philpott;
                        Anita Olsen;
                        Mary Tootham;
                        Vamsi K. Yadavalli;
                        Christopher J. Ehrhardt
Date Published

January 2021
Annotation
              The goal of this study was to characterize the reproducibility of extracellular and cell pellet associated DNA yields recovered from handled substrates.
Abstract
              Results showed that extracellular DNA yields were extremely variable between contributors—ranging between 0 and >10ng—and tended to dwarf cell pellet yields, which varied between 0 and ∼230 pg. DNA yields across multiple samples from the same contributor on different days showed similar levels of variability in both DNA fractions, indicating that extracellular DNA yield is largely influenced by extrinsic and/or environmental factors and is not a contributor-specific attribute. Microscopic surveys of cells from the pellet fraction as well as fingerprints from the same contributor samples were conducted following treatment with fluorescent DNA stain. Nearly all imaged cells exhibited diffuse fluorescence across the cell without discernable evidence of nuclei. This is consistent with the limited nature of DNA recovery from the pellet fraction and the prevalence of extracellular DNA in these samples. (Publisher Abstract)
      
  
          Additional Details
Grant Number(s)
        2017-DN-BX-0186


  
  
        
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  Publication Type
        Research (Applied/Empirical)


  
  
        
  Award
        Nanoscale Imaging and Chemical Analysis of Extracellular DNA in Trace Evidence Samples
    
  
  
Date Published: January 1, 2021
    
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