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                            Library & Multimedia
 In Vitro Isolation of Class-specific Oligonucleotide-based Small-Molecule Receptors
NCJ Number
              254114
Journal

*Nucleic Acids Research* Volume: 47 Issue: 12 Dated: July 2019 Pages: e71
Author(s)
          Weijuan Yang;
                        Haixiang Yu;
                        Obtin Alkhamis;
                        Yingzhu Liu;
                        Juan Canoura;
                        Fengfu Fu;
                        Yi Xiao
Date Published

July 2019
Length
              1 page
Annotation
              Since class-specific bioreceptors are highly desirable for recognizing structurally similar small molecules, but the generation of such affinity elements has proven challenging, the current research developed a novel 'parallel-and-serial' selection strategy for isolating class-specific oligonucleotide-based receptors (aptamers) in vitro.
Abstract
              This strategy first involves parallel selection to selectively enrich cross-reactive binding sequences, followed by serial selection that enriches aptamers binding to a designated target family. As a demonstration, a class-specific DNA aptamer was isolated against a family of designer drugs known as synthetic cathinones. The aptamer binds to 12 diverse synthetic cathinones with nanomolar affinity and does not respond to 11 structurally similar non-target compounds, some of which differ from the cathinone targets by a single atom. This is the first account of an aptamer exhibiting a combination of broad target cross-reactivity, high affinity, and remarkable. specificity. Leveraging the qualities of this aptamer, instantaneous colorimetric detection of synthetic cathinones at nanomolar concentrations in biological samples is achieved. Project findings significantly expand the binding capabilities of aptamers as class-specific bioreceptors and further demonstrate the power of rationally designed selection strategies for isolating customized aptamers with desired binding profiles. The researchers believe that this aptamer isolation approach can be broadly applied to isolate class-specific aptamers for various small molecule families. (publisher abstract modified)
      
  
          Additional Details
Grant Number(s)
        2016-DN-BX-0167
                                2015-R2-CX-0034


  
  
        
  Agencies
        NIJ-Sponsored


  
  
        
  Publication Type
        Research (Applied/Empirical)
                                Report (Study/Research)
                                Report (Grant Sponsored)
                                Program/Project Description


  
  
        
  Award
        Electrochemical and Colorimetric Aptamer-Based Sensors for Rapid On-site Screening of Synthetic Cannabinoids in Seized Substances and Biological samples.
                                Rapid and Accurate On-Site Screening for a Broad Range of Synthetic Cathinones by Electronic Drug Analyzer Based on a Cross-Reactive Aptamer
    
  
  
Date Published: July 1, 2019
    
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