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                            Library & Multimedia
 Verification of Protein Biomarker Specificity for the Identification of Biological Stains by Quadrupole Time-of-Flight Mass Spectrometry
NCJ Number
              252304
Journal

*ELECTROPHORESIS:* Volume: 38 Issue: 6 Dated: 2017
Author(s)
          Kevin M. Legg;
                        Roger  Powell;
                        Nichole  Reisdorph;
                        Rick  Reisdorph;
                        Philip B. Danielson
Date Published

March 2017
Length
              5 pages
Annotation
              This article reports on a project that developed a multiplex quadrupole time‐of‐flight mass spectrometry assay to verify the tissue/body fluid specificity the 23 protein biomarkers that comprise these panels and the consistency with which they can be detected across a sample population of 50 humans.
Abstract
              Advances in proteomics technology over the past decade offer forensic serologists a greatly improved opportunity to accurately characterize the tissue source from which a DNA profile has been developed. Such information can provide critical context to evidence and can help to prioritize downstream DNA analyses. Previous proteome studies compiled panels of "candidate biomarkers" specific to each of five body fluids (i.e., peripheral blood, vaginal/menstrual fluid, seminal fluid, urine, and saliva). In the current study, single‐source samples of these human body fluids were accurately identified by the detection of one or more high‐specificity biomarkers. Recovery of body fluid samples from a variety of substrates did not impede accurate characterization and, of the potential inhibitors assayed, only chewing tobacco juice appeared to preclude the identification of a target body fluid. Using a series of 2‐component mixtures of human body fluids, the multiplex assay accurately identified both components in a single‐pass. Only in the case of saliva and peripheral blood did matrix effects appear to impede the detection of salivary proteins. (Publisher abstract modified)
      
  
          Additional Details
Grant Number(s)
        2006-DN-BX-K001
                                2009-DN-BX-K165


  
  
        
  Agencies
        NIJ-Sponsored


  
  
        
  Publication Type
        Research (Applied/Empirical)
                                Report (Study/Research)
                                Report (Grant Sponsored)
                                Program/Project Description


  
  
        
  Award
        Isolation of Highly-Specific Protein Markers for the Identification of Biological Stains: Adapting Comparative Proteomics to Forensics
                                Validation of Highly-Specific Protein Markers for the Identification of Biological Stains
    
  
  
Date Published: March 1, 2017
    
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                Blood stains
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