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                            Library & Multimedia
 Surface enhanced Raman scattering specificity for detection and identification of dried bloodstains
NCJ Number
              303549
Journal

*Forensic Science International* Volume: 328 Dated: November 2021
Author(s)
          T. Reese;
                        C. Suarez;
                        W.R. Premasiri;
                        M.L. Shaine;
                        H. Ingraham;
                        A.N. Brodeur;
                        L.D. Ziegler
Date Published

November 2021
Annotation
              This article reports on a project that used surface enhanced Raman scattering specificity for the detection and identification of dried bloodstains.
Abstract
              Surface enhanced Raman spectroscopy (SERS) provides highly specific vibrational signatures that identify dried blood for a variety of forensic applications. SERS spectra on Au nanoparticle substrates excited at 785 nm were found to identify dried stains of human and non-human blood from seven animals and distinguish stains due to menstrual and peripheral blood. In addition, the unique SERS bloodstain spectrum was distinct from the SERS spectra of 30 red-brown stains of potential household substances that could be visually mistaken for bloodstains and from food stains that have been shown to give positive results with presumptive colorimetric blood tests. Finally, a SERS swab procedure has been developed and demonstrates that the substrates that a blood sample dried on does not offer any Raman or fluorescence interference for the SERS identification of dried blood. Such bloodstains on porous and nonporous materials are all identical and exclusively due to the heme moiety of hemoglobin. Optimized selection of the extraction solvent is found to control the chemical composition of molecular components appearing in the SERS spectrum of complex, multicomponent biological mixtures, such as body fluids. (publisher abstract modified)
      
  
          Additional Details
Grant Number(s)
        2018-MU-MU-0013


  
  
        
  Agencies
        NIJ-Sponsored


  
  
        
  Publication Type
        Research (Applied/Empirical)


  
  
        
  Award
        Body fluid analysis detection and identification by surface enhanced Raman spectroscopy for forensic scientists
    
  
  
Date Published: November 1, 2021
    
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