U.S. flag
      An official website of the United States government, Department of Justice.
        Here's how you know
      Here's how you know**Official websites use .gov**
            
            A **.gov** website belongs to an official government organization in the United States.
      **Secure .gov websites use HTTPS**
            
            A **lock** (
            A locked padlock
            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites. Menu

      
        close

            
            
                                              About NIJ
        
                    
  
          About NIJ
      
    
          The NIJ Director
          How NIJ Is Organized
          Mission and Guiding Principles
          Research and Development Process
          Strategic Framework
          Assessments of NIJ
          Annual Reports & Reports to Congress
          Staff Directory
          Funding & Awards
        
                    
  
          Funding & Awards
      
    
          Current Funding
          Forthcoming Funding
          Expired Funding
          Awards: Listing of Funded Projects
          Guidance for Applicants and Awardees
          Fellowship & Student Programs
          Travel Scholarships
          NIJ Challenge Program
          Library & Multimedia
        
                    
  
          Library & Multimedia
      
    
          NIJ Journal
          Podcasts
          Publications Listing
          Multimedia Listing
          Rated Practices on CrimeSolutions
          Rated Programs on CrimeSolutions
          Software & Databases
          Datasets
          Patents
          Topics
        
                    
  
          Topics
      
    
          Article Listing
          Corrections
          Courts
          Crime Prevention
          Crimes
          Drugs and Crime
          Equipment and Technology
          Forensic Sciences
          Youth Justice
          Justice System Reform
          Law Enforcement
          Tribal Crime and Justice
          Victims of Crime
          Events
        
                    
  
          Events
      
    
          NIJ 2024 Research Conference
          Scheduled Events
          On-Demand Events
          Research Meetings & Workshops
          NIJ-Hosted Online Training

                            
                                About
          Contact Us
          Subscribe
          Topics A-Z
                  Share
FacebookXLinkedInRedditPinterestEmail Home
                            Library & Multimedia
 West Virginia State Police Enhances STR Capabilities, Final Report
NCJ Number
              174807

Date Published

1998
Length
              13 pages
Annotation
              A grant was awarded by the National Institute of Justice to expand PCR-based capabilities and move the operation of the West Virginia State Police DNA laboratory closer to automation.
Abstract
              In order to maximize the information obtained from all routine types of evidence, additional methods of PCR-based analysis needed to be developed. The grant award was designed to validate additional short tandem repeat systems using the ABI Prism 377 Gene Sequencer. The necessary equipment was purchased, including the gene sequencer and a profiler PCR amplification kit. The validity of the technology selected was evaluated, and acceptable protocols for its use were developed. The following protocols related to amplification and run conditions for the profiler kit on the gene sequencer were developed: amplification and gel preparation, gel pouring, instrument set-up, sample and allelic ladder preparation, and sample loading and electrophoresis. Population samples selected from past validation studies were used to create a local database for the profiler system. Problems encountered during the course of expanding PCR- based capabilities and moving the DNA laboratory closer to automation are noted. Tables
      
  
          Additional Details
Grant Number(s)
        96-IJ-CX-0055


  
  
        
  Agencies
        NIJ-Sponsored
    
  
  
Date Published: January 1, 1998
    
          Downloads
        
                      *No download available*

Side navigation
  

        

  
  
              NIJ Journal
    
      
            
            
            
      Podcasts
    
      
            
            
            
      Publications Listing
    
      
            
            
            
      Multimedia Listing
    
      
            
            
            
      Rated Practices on CrimeSolutions
    
      
            
            
            
      Rated Programs on CrimeSolutions
    
      
            
            
            
      Software & Databases
    
      
            
            
            
      Datasets
    
      
            
            
            
      PatentsRelated Topics
        
                Computer aided operations
        Crime laboratories
        Criminal investigation
        Criminalistics
        DNA fingerprinting



  
Similar Publications
        
                      Technical Note – Direct Analysis In Real Time Mass Spectrometry (DART-MS)
                  dcDegenerate Oligonucleotide Primed-PCR for Multilocus, Genome-wide Analysis From Limited Quantities of DNA
                  A Feasibility Study of Direct Analysis in Real Time-Mass Spectrometry for Screening Organic Gunshot Residues from various Substrates
 Top

      National Institute of Justice

        
        
                        About NIJ
                    About NIJ
      
    
                  How NIJ Is Organized
      
    
                  NIJ Director
      
    
                  Research Disclaimer
      
    
                  Funding & Awards
                    Funding & Awards Home
      
    
                  Awards Listing
      
    
                  Current Funding
      
    
                  Guidance for Applicants and Awardees
      
    
                  Resources
                    Events
      
    
                  Podcasts
      
    
                  Publications
      
    
                  Topics
      
    
                  Multimedia
      
    
                  Reuse Policy
      
    
                  Contacts
                    Contact NIJ
      
    
                  Staff Directory
      
    
                  Submit a FOIA Request
      
    
                  File a Civil Rights ComplaintFacebook
          
                      X
          
                      YouTube
  

      810 7th St. NW, Washington, D.C. 20531
      
              Secondary Footer link menu
            Accessibility
              Plain Language
              Privacy Policy
              Legal Policies and Disclaimer
              No FEAR Act
              Freedom of Information Act
              USA.gov
              Justice.gov