U.S. flag
      An official website of the United States government, Department of Justice.
        Here's how you know
      Here's how you know**Official websites use .gov**
            
            A **.gov** website belongs to an official government organization in the United States.
      **Secure .gov websites use HTTPS**
            
            A **lock** (
            A locked padlock
            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites. Menu

      
        close

            
            
                                              About NIJ
        
                    
  
          About NIJ
      
    
          The NIJ Director
          How NIJ Is Organized
          Mission and Guiding Principles
          Research and Development Process
          Strategic Framework
          Assessments of NIJ
          Annual Reports & Reports to Congress
          Staff Directory
          Funding & Awards
        
                    
  
          Funding & Awards
      
    
          Current Funding
          Forthcoming Funding
          Expired Funding
          Awards: Listing of Funded Projects
          Guidance for Applicants and Awardees
          Fellowship & Student Programs
          Travel Scholarships
          NIJ Challenge Program
          Library & Multimedia
        
                    
  
          Library & Multimedia
      
    
          NIJ Journal
          Podcasts
          Publications Listing
          Multimedia Listing
          Rated Practices on CrimeSolutions
          Rated Programs on CrimeSolutions
          Software & Databases
          Datasets
          Patents
          Topics
        
                    
  
          Topics
      
    
          Article Listing
          Corrections
          Courts
          Crime Prevention
          Crimes
          Drugs and Crime
          Equipment and Technology
          Forensic Sciences
          Youth Justice
          Justice System Reform
          Law Enforcement
          Tribal Crime and Justice
          Victims of Crime
          Events
        
                    
  
          Events
      
    
          NIJ 2024 Research Conference
          Scheduled Events
          On-Demand Events
          Research Meetings & Workshops
          NIJ-Hosted Online Training

                            
                                About
          Contact Us
          Subscribe
          Topics A-Z
                  Share
FacebookXLinkedInRedditPinterestEmail Home
                            Library & Multimedia
 Y-Short Tandem Repeat Specific DNA Enhancement Strategy To Aid the Analysis of Late Reported (>= 6 days) Sexual Assault Cases
NCJ Number
              249425
Journal

*Medicine Science and the Law* Volume: 54 Issue: 4 Dated: October 2014 Pages: 209-218
Author(s)
          E. K.  Hanson;
                        J.  Ballantyne
Date Published

October 2014
Length
              10 pages
Annotation
              This study's objective was to develop additional strategies for the recovery of male semen donor DNA profiles from samples obtained approximately 6 days post-coitus.
Abstract
              The ability to obtain an autosomal short tandem repeat (STR) profile of the semen donor from the reproductive tract of a living victim rapidly diminishes as the post-coital interval increases. This is of concern where victims of sexual assault provide vaginal samples several days after the incident. In order to overcome the technological impediments inherent in autosomal DNA typing with extended interval samples, the authors previously employed the use of Y chromosome STR profiling which, by targeting only male DNA, can eliminate masking of the male profile (by the victim's alleles) or critical polymerase chain reaction reagent titration (due to excessive female DNA). Thus, by using Y-STR profiling and additional enhancement strategies, the authors reported the ability to recover Y-STR profiles from samples collected 5 to 6 days after intercourse; however, the reproductive biology literature indicates that spermatozoa are found in the human cervix up to 7 to 10 days post-coitus. Thus, even with improved extraction and profiling techniques, the authors failed to routinely recover profiles from samples collected approximately 6 days after intercourse. The current study, however, used nested polymerase chain reaction and DNA concentration procedures that together maximized the recovery and targeting of male DNA. The authors demonstrated the ability to obtain semen donor Y-STR profiles in extended interval post-coital samples collected 6 to 9 days after intercourse. This approaches the recognized time limits of sperm residence in the cervico-vaginal canal as described in the clinical literature. (Publisher abstract modified)
      
  
          Additional Details
Grant Number(s)
        2007-DN-BX-K147
                                2007-DN-BX-K148


  
  
        
  Agencies
        NIJ-Sponsored


  
  
        
  Publication Type
        Research (Applied/Empirical)
                                Report (Study/Research)
                                Report (Grant Sponsored)


  
  
        
  Award
        DNA Profiling of the Semen Donor in Extended Interval Post-Coital Samples
                                Y Chromosome Whole Genome Analysis Strategies: Improved Detection of Male DNA
    
  
  
Date Published: October 1, 2014
    
          Downloads
        
                      HTML

Side navigation
  

        

  
  
              NIJ Journal
    
      
            
            
            
      Podcasts
    
      
            
            
            
      Publications Listing
    
      
            
            
            
      Multimedia Listing
    
      
            
            
            
      Rated Practices on CrimeSolutions
    
      
            
            
            
      Rated Programs on CrimeSolutions
    
      
            
            
            
      Software & Databases
    
      
            
            
            
      Datasets
    
      
            
            
            
      PatentsRelated Topics
        
                Body fluid analysis
        Crisis response
        DNA typing
        Investigations
        Investigative techniques



  
Similar Publications
        
                      Stability and Hydrolysis of Desomorphine-Glucuronide
                  Distance to Trauma Centers Among Gunshot Wound Victims: Identifying Trauma 'Deserts' and 'Oases' in Detroit
                  Differentiation of the Regioisomeric 2 3 and 4-Trifluoromethylphenylpiperazines (TFMPP) by GC-IRD and GC-MS
 Top

      National Institute of Justice

        
        
                        About NIJ
                    About NIJ
      
    
                  How NIJ Is Organized
      
    
                  NIJ Director
      
    
                  Research Disclaimer
      
    
                  Funding & Awards
                    Funding & Awards Home
      
    
                  Awards Listing
      
    
                  Current Funding
      
    
                  Guidance for Applicants and Awardees
      
    
                  Resources
                    Events
      
    
                  Podcasts
      
    
                  Publications
      
    
                  Topics
      
    
                  Multimedia
      
    
                  Reuse Policy
      
    
                  Contacts
                    Contact NIJ
      
    
                  Staff Directory
      
    
                  Submit a FOIA Request
      
    
                  File a Civil Rights ComplaintFacebook
          
                      X
          
                      YouTube
  

      810 7th St. NW, Washington, D.C. 20531
      
              Secondary Footer link menu
            Accessibility
              Plain Language
              Privacy Policy
              Legal Policies and Disclaimer
              No FEAR Act
              Freedom of Information Act
              USA.gov
              Justice.gov