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                            Library & Multimedia
 Technique for Setting Analytical Thresholds in Massively Parallel Sequencing-based Forensic DNA Analysis
NCJ Number
              252462
Journal

*PLoS ONE* Volume: 12 Issue: 5 Dated: 2017
Author(s)
          Brian Young;
                        Jonathan L. King;
                        Bruce Budowle;
                        Luigi Armogida
Date Published

2017
Length
              5 pages
Annotation
              This article describes an approach for establishing objective analytical thresholds suitable for multiplexed amplicon (targeted) sequencing by massively parallel sequencing (PCR-MPS) methods, and a definition is proposed for PCR-MPS method background noise, along with a description of an analytical threshold based on background noise.
Abstract
              PCR-MPS may supplement or replace other instrumental analysis methods, such as capillary electrophoresis and Sanger sequencing, for STR and mitochondrial DNA typing, respectively. PCR-MPS also may enable the expansion of forensic DNA analysis methods to include new marker systems such as single nucleotide polymorphisms (SNPs) and insertion/deletions (indels) that currently are assayable using various instrumental analysis methods, including microarray and quantitative PCR. Acceptance of PCR-MPS as a forensic method will depend in part upon developing protocols and criteria that define the limitations of a method, including a defensible analytical threshold or method detection limit. (publisher abstract modified)
      
  
          Additional Details
Grant Number(s)
        2015-DN-BX-K067


  
  
        
  Agencies
        NIJ-Sponsored


  
  
        
  Publication Type
        Research (Applied/Empirical)
                                Report (Study/Research)
                                Report (Grant Sponsored)
                                Program/Project Description


  
  
        
  Award
        Enhancing Mixture Interpretation with Highly Informative STRs
    
  
  
Date Published: January 1, 2017
    
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