U.S. flag
      An official website of the United States government, Department of Justice.
        Here's how you know
      Here's how you know**Official websites use .gov**
            
            A **.gov** website belongs to an official government organization in the United States.
      **Secure .gov websites use HTTPS**
            
            A **lock** (
            A locked padlock
            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites. Menu

      
        close

            
            
                                              About NIJ
        
                    
  
          About NIJ
      
    
          The NIJ Director
          How NIJ Is Organized
          Mission and Guiding Principles
          Research and Development Process
          Strategic Framework
          Assessments of NIJ
          Annual Reports & Reports to Congress
          Staff Directory
          Funding & Awards
        
                    
  
          Funding & Awards
      
    
          Current Funding
          Forthcoming Funding
          Expired Funding
          Awards: Listing of Funded Projects
          Guidance for Applicants and Awardees
          Fellowship & Student Programs
          Travel Scholarships
          NIJ Challenge Program
          Library & Multimedia
        
                    
  
          Library & Multimedia
      
    
          NIJ Journal
          Podcasts
          Publications Listing
          Multimedia Listing
          Rated Practices on CrimeSolutions
          Rated Programs on CrimeSolutions
          Software & Databases
          Datasets
          Patents
          Topics
        
                    
  
          Topics
      
    
          Article Listing
          Corrections
          Courts
          Crime Prevention
          Crimes
          Drugs and Crime
          Equipment and Technology
          Forensic Sciences
          Youth Justice
          Justice System Reform
          Law Enforcement
          Tribal Crime and Justice
          Victims of Crime
          Events
        
                    
  
          Events
      
    
          NIJ 2024 Research Conference
          Scheduled Events
          On-Demand Events
          Research Meetings & Workshops
          NIJ-Hosted Online Training

                            
                                About
          Contact Us
          Subscribe
          Topics A-Z
                  Share
FacebookXLinkedInRedditPinterestEmail Home
                            Library & Multimedia
 Systematic analysis of novel psychoactive substances. II. Development of a screening/confirmatory LC-QqQ-MS/MS method for 800
NCJ Number
              302573
Journal

*Forensic Chemistry* Volume: 16 Dated: 2019
Author(s)
          A. N. Kimble;
                        et al
Date Published

2019
Annotation
              This report describes the development of a dilute-and-shoot LC-QqQ-MS/MS screening method for urine using dynamic multiple reaction monitoring (dMRM) mode capable of detecting 826 NPS and other licit and illicit analytes.
Abstract
              The presence of novel psychoactive substances (NPS) in forensic casework world-wide has steadily increased over the past decades. NPS pose difficulties for detection, since there are many generations and structural variations circulating in communities and the illicit market at any given time. There are currently no validated screening or quantitation methods available that encompass more than a limited number of NPS. The use of a liquid chromatography triple quadrupole tandem mass spectrometry (LC-QqQ-MS/MS) targeted method allows for the detection of a large number of NPS, mainly due to the high selectivity and sensitivity of this approach; however, a major challenge with developing such a comprehensive method is that full validation can be extremely tedious and time consuming. The work presented in the current article employs a series of non-coeluting NPS standard mixtures to facilitate method validation. The method was fully validated according to accepted guidelines for quantitative analysis of 80 NPS using three non-coeluting standard NPS mixtures. Limits of detection and quantification were in the 0.01–0.36 ng/g range for all analytes. In addition, a qualitative screen capable of identifying all 826 analytes was developed. The quantitative method was successfully applied to a set of blind spiked urine specimens, while the qualitative screen was effective in identifying NPS and other relevant analytes in a series of authentic urine specimens. (publisher abstract modified)
      
  
          Additional Details
Grant Number(s)
        2014-R2-CX-K006
                                2017-IJ-CX-0004


  
  
        
  Agencies
        NIJ-Sponsored


  
  
        
  Award
        Forensic Toxicological Screening/Confirmation of 500+ Designer Drugs by LC-QTOF-MS and LC-QqQ-MS Analysis
                                Development of Improved Extraction/Purification Methods for LC-QqQ-MS Analysis of Novel Psychoactive Substances
    
  
  
Date Published: January 1, 2019
    
          Downloads
        
                      HTML

Side navigation
  

        

  
  
              NIJ Journal
    
      
            
            
            
      Podcasts
    
      
            
            
            
      Publications Listing
    
      
            
            
            
      Multimedia Listing
    
      
            
            
            
      Rated Practices on CrimeSolutions
    
      
            
            
            
      Rated Programs on CrimeSolutions
    
      
            
            
            
      Software & Databases
    
      
            
            
            
      Datasets
    
      
            
            
            
      PatentsRelated Topics
        
                Forensic sciences
        Chromatography
        Mass spectrometry
        Novel psychoactive substances
        Crime laboratories



  
Similar Publications
        
                      White Paper – Information Sharing Between Medicolegal Death Investigation Offices And Organ And Tissue Recovery Organizations
                  White Paper – Information Sharing Between Medicolegal Death Investigators And Toxicology Testing Services
                  Transfer and Persistence Studies of Inorganic and Organic Gunshot Residues using Synthetic Skin Membranes
 Top

      National Institute of Justice

        
        
                        About NIJ
                    About NIJ
      
    
                  How NIJ Is Organized
      
    
                  NIJ Director
      
    
                  Research Disclaimer
      
    
                  Funding & Awards
                    Funding & Awards Home
      
    
                  Awards Listing
      
    
                  Current Funding
      
    
                  Guidance for Applicants and Awardees
      
    
                  Resources
                    Events
      
    
                  Podcasts
      
    
                  Publications
      
    
                  Topics
      
    
                  Multimedia
      
    
                  Reuse Policy
      
    
                  Contacts
                    Contact NIJ
      
    
                  Staff Directory
      
    
                  Submit a FOIA Request
      
    
                  File a Civil Rights ComplaintFacebook
          
                      X
          
                      YouTube
  

      810 7th St. NW, Washington, D.C. 20531
      
              Secondary Footer link menu
            Accessibility
              Plain Language
              Privacy Policy
              Legal Policies and Disclaimer
              No FEAR Act
              Freedom of Information Act
              USA.gov
              Justice.gov