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                            Library & Multimedia
 Simplified COI barcoding of blow, flesh, and scuttle flies encountered in medicolegal investigations
NCJ Number
              307949
Journal

*Forensic Science Medicine and Pathology* Volume: Online Dated: 2023
Author(s)
          Sam C. Kwiatkowski;
                        Michelle R. Sanford;
                        Michael Donley;
                        Katherine Welch;
                        Roger Kahn
Date Published

2023
Annotation
              In this article, the authors demonstrate the correct identification of larvae, pupae, and pupal exuviae from the medicolegal casework for members of the blow fly, the flesh fly, and the scuttle fly genera.
Abstract
              Accurate insect identification is critical to the estimation of time of colonization (TOC) and post-mortem interval (PMI) in medicolegal death investigations. DNA testing is advantageous because it enables the identification of immature specimens that may not be identified based on morphology alone. In this paper, the authors describe a simplified DNA barcoding method for identifying relevant species that may be implemented by forensic genetics laboratories. A cytochrome oxidase (COI) fragment is analyzed after PCR amplification with a single primer set. The method is effective for many species commonly encountered in death investigations in the USA: members of blow fly genera*Calliphora, Chrysomya, Cochliomyia, Lucilia*, and*Phormia*; members of the flesh fly genera*Blaesoxipha, Oxysarcodexia, Ravinia*, and*Sarcophaga*; and the scuttle fly*Megaselia scalaris*. The authors tested the method on specimens with verified identifications and used it to build a collection of reference sequences from specimens collected in Harris County, Texas. They show here the correct identification of larvae, pupae, and pupal exuviae from the medicolegal casework. Publisher Abstract Provided
      
  
          Additional Details
Grant Number(s)
        2019-DU-BX-0022


  
  
        
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        NIJ-Sponsored


  
  
        
  Publication Type
        Research (Applied/Empirical)


  
  
        
  Award
        A DNA Barcoding Strategy for Blow and Flesh Flies Encountered During Medicolegal Casework
    
  
  
Date Published: January 1, 2023
    
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