U.S. flag
      An official website of the United States government, Department of Justice.
        Here's how you know
      Here's how you know**Official websites use .gov**
            
            A **.gov** website belongs to an official government organization in the United States.
      **Secure .gov websites use HTTPS**
            
            A **lock** (
            A locked padlock
            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites. Menu

      
        close

            
            
                                              About NIJ
        
                    
  
          About NIJ
      
    
          The NIJ Director
          How NIJ Is Organized
          Mission and Guiding Principles
          Research and Development Process
          Strategic Framework
          Assessments of NIJ
          Annual Reports & Reports to Congress
          Staff Directory
          Funding & Awards
        
                    
  
          Funding & Awards
      
    
          Current Funding
          Forthcoming Funding
          Expired Funding
          Awards: Listing of Funded Projects
          Guidance for Applicants and Awardees
          Fellowship & Student Programs
          Travel Scholarships
          NIJ Challenge Program
          Library & Multimedia
        
                    
  
          Library & Multimedia
      
    
          NIJ Journal
          Podcasts
          Publications Listing
          Multimedia Listing
          Rated Practices on CrimeSolutions
          Rated Programs on CrimeSolutions
          Software & Databases
          Datasets
          Patents
          Topics
        
                    
  
          Topics
      
    
          Article Listing
          Corrections
          Courts
          Crime Prevention
          Crimes
          Drugs and Crime
          Equipment and Technology
          Forensic Sciences
          Youth Justice
          Justice System Reform
          Law Enforcement
          Tribal Crime and Justice
          Victims of Crime
          Events
        
                    
  
          Events
      
    
          NIJ 2024 Research Conference
          Scheduled Events
          On-Demand Events
          Research Meetings & Workshops
          NIJ-Hosted Online Training

                            
                                About
          Contact Us
          Subscribe
          Topics A-Z
                  Share
FacebookXLinkedInRedditPinterestEmail Home
                            Library & Multimedia
 Species and Age Determination of Blow Fly Pupae Based Upon Headspace Analysis
NCJ Number
              252744

Author(s)
          John V. Goodpaster
Date Published

January 2017
Length
              45 pages
Annotation
              This project's goal was to develop a chemical method for determining the species and age of forensically relevant pupae, i.e.. blow fly (fly) pupae, as well as to determine the effects of biotic and abiotic factors on the resulting chemical profiles.
Abstract
              Chemical analysis of fly pupae was conducted, with attention to chemical markers of species and age. Also studied were the effect of genetic variation, temperature, humidity, light level, and diet. Chemo metric techniques capable of identifying patterns in the data and classifying unknown samples were used for the discrimination and classification of data. The six phases of research design and methods are described. The project succeeded in developing a new method for the analysis of pupal liquid extracts by total vaporization solid phase micro extraction (TV-SPME), with gas chromatography-mass spectrometry (GC-MS) serving as the instrumentation for analysis. The TV-SPME delivery technique renders approximately an order of magnitude improvement in sensitivity over traditional liquid injection methods, This may alleviate the need for rotary evaporation, reconstitution, collection of high performance liquid chromatography fractions, and many of the other pre-concentration steps that are common in the current literature. Attempts to analyze the volatile organic compounds (VOCs) of Phaormia regina pupae via HS-SPME were unsuccessful. For useful chemical information to be obtained, the analysis had to be preceded by an extraction to separate the fatty acids, hydrocarbons, sterols, and other compounds of interest from the matrix. This led to the development for the analysis of pupal liquid extracts by TV-SPME. 8 tables, 7 figures, and 67 references
      
  
          Additional Details
Grant Number(s)
        2013-DN-BX-K019


  
  
        
  Agencies
        NIJ-Sponsored


  
  
        
  Publication Type
        Research (Applied/Empirical)
                                Report (Study/Research)
                                Report (Grant Sponsored)
                                Program/Project Description


  
  
        
  Award
        Species and age determination of blow fly pupae based upon headspace analysis
    
  
  
Date Published: January 1, 2017
    
          Downloads
        
                      PDF

Side navigation
  

        

  
  
              NIJ Journal
    
      
            
            
            
      Podcasts
    
      
            
            
            
      Publications Listing
    
      
            
            
            
      Multimedia Listing
    
      
            
            
            
      Rated Practices on CrimeSolutions
    
      
            
            
            
      Rated Programs on CrimeSolutions
    
      
            
            
            
      Software & Databases
    
      
            
            
            
      Datasets
    
      
            
            
            
      PatentsRelated Topics
        
                Crime Scene
        Death investigations
        Environmental influences
        Forensic entomology
        Forensic laboratories



  
Similar Publications
        
                      A Diagnosis of the Primary Difference between EuroForMix and STRmixTM
                  What FSSP Leaders Should Know About Artificial Intelligence And Its Application To Forensic Science In-Brief
                  Attitudes of Reporting Officers Extracted From Incident Reports Can Affect Rape Case Outcomes
 Top

      National Institute of Justice

        
        
                        About NIJ
                    About NIJ
      
    
                  How NIJ Is Organized
      
    
                  NIJ Director
      
    
                  Research Disclaimer
      
    
                  Funding & Awards
                    Funding & Awards Home
      
    
                  Awards Listing
      
    
                  Current Funding
      
    
                  Guidance for Applicants and Awardees
      
    
                  Resources
                    Events
      
    
                  Podcasts
      
    
                  Publications
      
    
                  Topics
      
    
                  Multimedia
      
    
                  Reuse Policy
      
    
                  Contacts
                    Contact NIJ
      
    
                  Staff Directory
      
    
                  Submit a FOIA Request
      
    
                  File a Civil Rights ComplaintFacebook
          
                      X
          
                      YouTube
  

      810 7th St. NW, Washington, D.C. 20531
      
              Secondary Footer link menu
            Accessibility
              Plain Language
              Privacy Policy
              Legal Policies and Disclaimer
              No FEAR Act
              Freedom of Information Act
              USA.gov
              Justice.gov