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                            Library & Multimedia
 Simultaneous Detection of Human Mitochondrial DNA and Nuclear Inserted Mitochondrial-Origin Sequences (NumtS) Using Forensic mtDNA Amplification Strategies and Pyrosequencing Technology
NCJ Number
              255545
Journal

*Journal of Forensic Sciences* Dated: 2012
Author(s)
          B. Bintz;
                        G. Dixon;
                        M. R. Wilson
Date Published

2012
Length
              0 pages
Annotation
              Since next-generation sequencing technologies enable the identification of minor mitochondrial DNA variants with higher sensitivity than Sanger methods, enabling improved identification of minor variants, the current study subjected mixtures of human mtDNA control region amplicons to pyrosequencing in determining the detection threshold of the Roche GS Junior® instrument (Roche Applied Science, Indianapolis, IN).
Abstract
              In addition to expected variants, a set of reproducible variants was consistently found in reads from one particular amplicon. A BLASTn search of the variant sequence revealed identity to a segment of a 611-bp nuclear insertion of the mitochondrial control region (NumtS) spanning the primer-binding sites of this amplicon (Nature 1995;378:489). Primers (Hum Genet 2012;131:757; Hum Biol 1996;68:847) flanking the insertion were used to confirm the presence or absence of the NumtS in buccal DNA extracts from twenty donors. These results further our understanding of human mtDNA variation and are expected to have a positive impact on the interpretation of mtDNA profiles using deep-sequencing methods in casework. (publisher abstract modified)
      
  
          Additional Details
Grant Number(s)
        2010-DN-BX-K171


  
  
        
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        Assessing Deep Sequencing Technology for Human Forensic mitochondrial DNA
    
  
  
Date Published: January 1, 2012
    
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        DNA (Deoxyribonucleic Acid)
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        Mitochondrial DNA



  
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