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                            Library & Multimedia
 Quadruplex Real-Time qPCR Assay for the Simultaneous Assessment of Total Human DNA, Human Male DNA, DNA Degradation and the Presence of PCR Inhibitors in Forensic Samples: A Diagnostic Tool for STR Typing
NCJ Number
              222071
Journal

*Forensic Science International: Genetics* Volume: 2 Issue: 2 Dated: March 2008 Pages: 108-125
Author(s)
          William R. Hudlow;
                        Mavis Date Chong;
                        Katie L. Swango;
                        Mark D. Timken;
                        Martin R. Buoncristiani
Date Published

March 2008
Length
              18 pages
Annotation
              This paper reports on the developmental validation of a quadruplex real-time qPCR assay (analysis) for the concurrent assessment of the total human DNA, human male DNA, DNA degradation and the presence of PCR inhibitors in forensic samples.
Abstract
              The ability to accurately determine the quantity of DNA present in a forensic sample and simultaneously determine the most appropriate typing system for each sample may result in substantial increase in laboratory efficiency and a more prudent use of limited samples. The nuTH01-nuSRY-nuCSF-IPC quadruplex qPCR assay has been shown to provide suitable quantifications for both total human and total human male DNA while providing qualitative information about the level of degradation and the presence of PCR inhibitors. The results from the nuTH01-nuSRY-nuSRY-nuCSF-IPC quadruplex qPCR assay can also be used to confirm the results of short tandem repeat (STR) typing, which may present unnecessary reanalysis of degraded or inhibited samples that have already yielded the maximum amount of information available. The increasing number of DNA typing system and decreasing amount of DNA per evidence item submitted to forensic laboratories has driven the development of several real-time quantitative PCR (qPCR) analyses capable of simultaneously estimating total human DNA and male DNA or of estimating total human DNA and extent of DNA degradation. This paper describes the quadruplex real-time qPCR assay developed to simultaneously assess total human DNA, human male DNA, DNA degradation, and PCR inhibitors in forensic samples. Tables, figures, references
      
  
          Additional Details
Grant Number(s)
        2002-IJ-CX-K008


  
  
        
  Agencies
        NIJ-Sponsored


  
  
        
  Publication Type
        Research (Applied/Empirical)
    
  
  
Date Published: March 1, 2008
    
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