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                            Library & Multimedia
 Quantification of fentanyl analogs in oral fluid using LC-QTOF-MS
NCJ Number
              304286
Journal

*Journal of Forensic Sciences* Volume: 66 Issue: 5 Dated: September 2021 Pages: 1871-1878
Author(s)
          K. B. Palmquist;
                        M. J. Swortwood
Date Published

July 2021
Annotation
              The purpose of this study was to develop and validate a quantitative method for fentanyl analogs in oral fluid (collected via QuantisalTM) using liquid chromatography-quadrupole-time-of-flight-mass spectrometry (LC-QTOF-MS).
Abstract
              Oral fluid is a valuable alternative matrix for forensic toxicologists due to ease of observed collection, limited biohazardous exposure, and indications of recent drug use. Limited information is available for fentanyl analog prevalence, interpretation, or analysis in oral fluid. With increasing numbers of fentanyl-related case of driving under the influence of a drug (DUID) cases appearing in the United States, the development of detection methods is critical. In the current study, validation resulted in limits of detection and quantification ranging from 0.5 to 1 ng/mL. Established linear range was 1–100 ng/mL for all analytes, except acetyl fentanyl at 0.5–100 ng/mL (R2 > 0.994). Within- and between-run precision and bias were considered acceptable with maximum values of ±15.2 percent CV and ±14.1 percent, respectively. Matrix effects exhibited ionization enhancement for all analytes with intensified enhancement at a low concentration (9.3–47.4 percent). No interferences or carryover was observed. Fentanyl analogs were stable in processed extracts stored in the autosampler (40C) for 48 hours. The validated method was used to quantify fentanyl analogs in authentic oral fluid samples (n=17) from probationers/parolees. Fentanyl and 4-ANPP concentrations were 1.0–104.5 ng/mL and 1.2–5.7 ng/mL, respectively. (publisher abstract modified)
      
  
          Additional Details
Grant Number(s)
        2017-R2-CX-0019


  
  
        
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        NIJ-Sponsored


  
  
        
  Publication Type
        Research (Applied/Empirical)


  
  
        
  Award
        Prevalence of Novel Psychoactive Substances in Oral Fluid
    
  
  
Date Published: July 1, 2021
    
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