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                            Library & Multimedia
 TLC-SERS study of Syrian rue (Peganum harmala) and its main alkaloid constituents
NCJ Number
              304169
Journal

*Journal of Raman Spectroscopy* Volume: 44 Issue: 1 Dated: 2012 Pages: 102-107
Author(s)
          Federica Pozzi;
                        et al
Date Published

2012
Length
              6 pages
Annotation
              This article reports on a project that examined the coupling of thin layer chromatography (TLC) and SERS as a tool for the separation and identification of four alkaloids, namely harmalol, harmaline, harmane and harmine, from the seed extract of Syrian rue (Peganum harmala).
Abstract
              Surface-enhanced Raman spectroscopy (SERS) has found increasing acceptance in art conservation and forensic science for its potential in detecting trace amounts of material; however, SERS is not a separation technique; therefore, it is not always suitable for distinguishing different components in a mixture. The alkaloids contained in the plant examined in the current project have historically been used as a dye and for medicinal purposes; they have recently drawn attention due to their antitumor activity. The use of TLC over high-performance liquid chromatography (HPLC) is a convenient way to reduce the amount of material, equipment, and time needed for the analysis; and coupling of TLC with SERS provides vibrational information on each compound in the mixture. HPLC analyses with diode array detection were also conducted as a test of the technique used to ascertain the composition of Syrian rue extract and validate the results obtained from TLC-SERS investigations. In addition, Syrian rue extract and its commercial alkaloid components were characterized by SERS and normal Raman spectroscopy for the first time, in order to provide valuable reference data to be used for identification purposes. (publisher abstract modified)
      
  
          Additional Details
Grant Number(s)
        2006-DN-BX-K034


  
  
        
  Agencies
        NIJ-Sponsored


  
  
        
  Publication Type
        Research (Applied/Empirical)


  
  
        
  Award
        Development of Advanced Raman Spectroscopy Methods and Databases for the Evaluation of Trace Evidence and the Examination of Questioned Documents
    
  
  
Date Published: January 1, 2012
    
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