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                            Library & Multimedia
 STR Melt Curve Genotyping Assay for Forensic Analysis Employing an Intercalating Dye Probe FRET
NCJ Number
              233655
Journal

*Journal of Forensic Sciences* Volume: 56 Issue: 1 Dated: January 2011 Pages: 36-45
Author(s)
          Micah D. Halpern, M.S.;
                        Jack Ballantyne, Ph.D.
Date Published

January 2011
Length
              10 pages
Annotation
              This study focused on an advancement within the category of hybridization-based detection.
Abstract
              The most common markers used in forensic genetics are short tandem repeats (STRs), the alleles of which are separated and analyzed by length using capillary electrophoresis (CE). In this work, proof of concept of a unique STR genotyping approach has been demonstrated using asymmetric PCR and a fluorescence resonance energy transfer (FRET)-based hybridization analysis that combines fluorophore-labeled allele-specific probes and a DNA intercalating dye (dpFRET) in a melt match/mismatch analysis format. The system was successfully tested against both a simple (TPOX) and a complex (D3S1358) loci, demonstrated a preliminary detection limit of less than 10 genomic equivalents with no allelic dropout and mixture identification in both laboratory-generated and clinical samples. With additional development, this approach has the potential to contribute to advancing the use of STR loci for forensic applications and related fields. (Published Abstract) Table, figures, and references
      
  
          Additional Details
Grant Number(s)
        2008-DN-BX-K012


  
  
        
  Agencies
        NIJ-Sponsored


  
  
        
  Publication Type
        Test/Measurement
                                Report (Study/Research)


  
  
        
  Award
        Rapid STR Prescreening of Forensic Samples at the Crime Scene
    
  
  
Date Published: January 1, 2011
    
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