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                            Library & Multimedia
 Pyrosequencing: Current forensic methodology and future applications-a review
NCJ Number
              305457
Journal

*Electrophoresis* Volume: Online Dated: September 2022
Author(s)
          Mirna Ghemrawi;
                        Nicole Fernandez Tejero;
                        George Duncan;
                        Bruce McCord
Date Published

September 2022
Annotation
              This review explains the pyrosequencing workflow and illustrates the step-by-step chemistry, followed by a description of the assay design and factors to keep in mind for an exemplary assay.
Abstract
              The recent development of small, single-amplicon-based benchtop systems for pyrosequencing has opened up a host of novel procedures for applications in forensic science. Pyrosequencing is a sequencing by synthesis technique, based on chemiluminescent inorganic pyrophosphate detection. Existing and potential forensic applications are highlighted using this technology. Current applications include identifying species, identifying bodily fluids, and determining smoking status. We also review progress in potential applications for the future, including research on distinguishing monozygotic twins, detecting alcohol and drug abuse, and other phenotypic characteristics such as diet and body mass index. Overall, the versatility of the pyrosequencing technologies renders it a useful tool in forensic genomics. (Publisher abstract provided)
      
  
          Additional Details
Grant Number(s)
        2020-DQ-BX-0015


  
  
        
  Agencies
        NIJ-Sponsored


  
  
        
  Publication Type
        Research (Applied/Empirical)


  
  
        
  Award
        Comparative Assessment of Emerging Technologies for Body Fluid Identification
    
  
  
Date Published: September 1, 2022
    
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      PatentsRelated Topics
        
                Forensic sciences
        Pyrolysis
        Research and Development in Forensic Science for Criminal Justice Purposes
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        Alcohol abuse



  
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