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                            Library & Multimedia
 Sample Collection System for DNA Analysis of Forensic Evidence: Towards Practical, Fully-Integrated STR Analysis
NCJ Number
              236826

Author(s)
          Eugene  Tan
Date Published

January 2010
Length
              45 pages
Annotation
              This research developed a sample collection system for law enforcement agents to use in collecting, protecting, and documenting biological evidence; and it performed initial processing steps in a format compatible with rapid sample-in to results-out microfluidic DNA analysis.
Abstract
              The sample collection system developed consists of an evidence-collection device (a collecting swab and accompanying tube for swab insertion) and a sample processing cartridge. This "Smart Cartridge" lyses cells, makes soluble and concentrates DNA, and transfers the DNA solution to a microfluidic biochip. The microfluidic biochip will process the DNA solution to generate purified DNA compatible with the requirements for STR analysis. The evidence-collection device is designed to accept blood, buccal cells, saliva, and cellular samples. Cotton, modified cellulose, foam, nylon, polyester, and rayon-tipped swabs were evaluated for reproducibility of cell collection, ease of collection, tolerance to collection, protocol, liquid absorption, and use in forensic applications. The Bode SecurSwab was selected for the sample collection system. Operation of the system requires the user to collect a swab sample, insert the swab into the collection tube, place the tube into the Smart Cartridge, and press a start button; no further intervention is required. The DNA generated by the sample collection system will then be transferred automatically to NetBio's fully integrated microfluidic biochip for STR typing, which will generate an STR profile in 45 minutes from sample introduction, with minimal operating requirements. Several approaches to the sample processing steps were evaluated, and those selected were integrated to develop an optimized Smart Cartridge. 25 figures and 13 references
      
  
          Additional Details
Grant Number(s)
        2008-DN-BX-K010


  
  
        
  Agencies
        NIJ-Sponsored


  
  
        
  Publication Type
        Report (Study/Research)


  
  
        
  Award
        NIJ Forensic DNA R&DNational Institute of Justice (NIJ''s) Forensic DNA Reserach and DevelopmentSample Collection Systems for DNA Analysis of Forensic Evidence Samples1/1/09 - 12/31/09
    
  
  
Date Published: January 1, 2010
    
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