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                            Library & Multimedia
 A method to generate capture baits for targeted sequencing
NCJ Number
              307120
Journal

*Nucleic Acids Research* Dated: 2013
Author(s)
          Balaji Sundararaman;
                        Alisa O. Vershinina;
                        Samantha Hershauer;
                        Joshua D. Kapp;
                        Shelby Dunn;
                        Beth Shapiro;
                        Richard E. Green
Date Published

2023
Length
              11 pages
Annotation
              In this paper, the authors demonstrate the use of the CNER method for targeted genotyping by producing a set of CNER probes to capture 23771 SNPs in the horse genome, using ten ancient horse DNA libraries of varying endogenous DNA content and DNA degradation levels, and show that the CNERs effectively perform target enrichment even in highly degraded ancient samples comparably.
Abstract
              Hybridization capture approaches allow targeted high-throughput sequencing analysis at reduced costs compared to shotgun sequencing. Hybridization capture is particularly useful in analyses of genomic data from ancient, environmental, and forensic samples, where target content is low, DNA is fragmented and multiplex PCR or other targeted approaches often fail. Here, the authors describe a DNA bait synthesis approach for hybridization capture that they call *C*ircular *N*ucleic acid *E*nrichment *R*eagent, or CNER (pronounced ‘*snare’*). The CNER method uses rolling-circle amplification followed by restriction digestion to discretize microgram quantities of hybridization probes. The authors demonstrate the utility of the CNER method by generating probes for a panel of 23 771 known sites of single nucleotide polymorphism in the horse genome. Using these probes, they capture and sequence from a panel of ten ancient horse DNA libraries, comparing CNER capture efficiency to a commercially available approach. With about one million read pairs per sample, CNERs captured more targets (90.5% versus 66.5%) at greater mean depth than an alternative commercial approach. (Publisher Abstract Provided)
      
  
          Additional Details
Grant Number(s)
        2020-R2-CX-0029
                                2020-DQ-BX-0014


  
  
        
  Agencies
        NIJ-Sponsored


  
  
        
  Publication Type
        Research (Applied/Empirical)


  
  
        
  Award
        Develop non-PCR-based target enrichment reagents and methods for MPS analysis of forensic DNA
                                Evaluation of nuclear DNA from rootless hair for forensic purposes
    
  
  
Date Published: January 1, 2023
    
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      PatentsRelated Topics
        
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        DNA (Deoxyribonucleic Acid)



  
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