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                            Library & Multimedia
 Potency testing of up to sixteen cannabinoids in hemp-infused edibles using liquid chromatography diode array detector with optional confirmation of identity by electrospray ionization time-of-flight mass spectrometry
NCJ Number
              307430

Author(s)
          Liguo Song;
                        Grant Meyer;
                        Emmanuel Adejumo;
                        Emily Jovanovich;
                        Lindsey LeBlanc;
                        Jake Provis
Date Published

2023
Annotation
              The objective of this study was to first develop and validate an LC-DAD method for potency testing of a minimum of fourteen cannabinoids recommended by the AOAC international, and then examine the method capability in the analysis of a broad range of hemp-infused edibles in the marketplace; the authors report solving several related problems, studying the use of methanol as an alternative solvent, and developing corresponding extraction procedures for hemp-infused edibles.
Abstract
              The authors report on their development and validation of an LC-DAD method for potency testing of up to sixteen cannabinoids; they also discuss the method’s application for analysis of twenty hemp-infused edibles encompassing a broad range of complex matrices. The method was validated according to ISO 17025 guidelines and met requirements. The authors extracted samples or their uniform water-dispersions, by methanol, under homogenization through pulverization and/or ultrasonication. By spiking abnormal cannabidiol, a cannabinoid not naturally present in hemp, into each sample, extraction recovery was tracked in real time, obtaining 90-108 percent in triplicates. The linear calibration range was between 0.008 and 10 percent (w/w) for each cannabinoid using a 250 μg/mL solution of hemp-infused edibles, except for drinks (sparkling water and tea), where it was between 0.0008 and 1 percent (w/w) using a 2.5 mg/mL solution. ESI/TOFMS confirmed a good method specificity, i.e., without any false positive identification of individual cannabinoid. Publisher Abstract Provided
      
  
          Additional Details
Grant Number(s)
        2020-DQ-BX-0021


  
  
        
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  Award
        Validation of a LC-DAD-ESI/MS/MS method for the accurate measurement of THC and THCA-A among twenty cannabinoids in various products of Cannabis
    
  
  
Date Published: January 1, 2023
    
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