# Patent

## Title
Prostaglandin derivatives

## Abstract

Prostaglandin nitroderivatives having improved pharmacological activity and enhanced tolerability are described. They can be employed for the treatment of glaucoma and ocular hypertension.

## Background
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a Continuation Application from U.S. Ser. No. 11/841,628, filed Aug. 20, 2007 (allowed), which is a Continuation Application from U.S. Ser. No. 11/029,698, filed Jan. 5, 2005 (U.S. Pat. No. 7,273,946), which claims the benefit of priority of European Patent Application no. 04100001.9, filed Jan. 5, 2004. The disclosure of each of these applications is incorporated herein.


BACKGROUND OF THE INVENTION
The present invention relates to new prostaglandin derivatives. More particularly, the present invention relates to prostaglandin nitrooxyderivatives, pharmaceutical compositions containing them and their use as drugs for treating glaucoma and ocular hypertension.
Glaucoma is optic nerve damage, often associated with increased intraocular pressure (IOP), that leads to progressive, irreversible loss of vision.
Almost 3 million people in the United States and 14 million people worldwide have glaucoma; this is the third leading cause of blindness worldwide.
Glaucoma occurs when an imbalance in production and drainage of fluid in the eye (aqueous humor) increases eye pressure to unhealthy levels.
It is known that elevated IOP can be at least partially controlled by administering drugs which either reduce the production of aqueous humor within the eye or increase the fluid drainage, such as beta-blockers, α-agonists, cholinergic agents, carbonic anhydrase inhibitors, or prostaglandin analogs.
Several side effects are associated with the drugs conventionally used to treat glaucoma.
Topical beta-blockers show serious pulmonary side effects, depression, fatigue, confusion, impotence, hair loss, heart failure and bradycardia.
Topical α-agonists have a fairly high incidence of allergic or toxic reactions; topical cholinergic agents (miotics) can cause visual side effects.
The side effects associated with oral carbonic anhydrase inhibitors include fatigue, anorexia, depression, paresthesias and serum electrolyte abnormalities (The Merck Manual of Diagnosis and Therapy, Seventeenth Edition, M. H. Beers and R. Berkow Editors, Sec. 8, Ch. 100).
Finally, the topical prostaglandin analogs (bimatoprost, latanoprost, travoprost and unoprostone) used in the treatment of glaucoma, can produce ocular side effects, such as increased pigmentation of the iris, ocular irritation, conjunctival hyperaemia, iritis, uveitis and macular oedema (Martindale, Thirty-third edition, p. 1445).
U.S. Pat. No. 3,922,293 describes monocarboxyacylates of prostaglandins F-type and their 15β isomers, at the C-9 position, and processes for preparing them; U.S. Pat. No. 6,417,228 discloses 13-aza prostaglandins having functional PGF2α receptor agonist activity and their use in treating glaucoma and ocular hypertension.
WO 90/02553 discloses the use of prostaglandins derivatives of PGA, PGB, PGE and PGF, in which the omega chain contains a ring structure, for the treatment of glaucoma or ocular hypertension.
WO 00/51978 describes novel nitrosated and/or nitrosylated prostaglandins, in particular novel derivatives of PGE1, novel compositions and their use for treating sexual dysfunctions.
U.S. Pat. No. 5,625,083 discloses dinitroglycerol esters of prostaglandins which may be used as vasodilators, antihypertensive cardiovascular agents or bronchodilators.
U.S. Pat. No. 6,211,233 discloses compounds of the general formula A-X1—NO2, wherein A contains a prostaglandin residue, in particular PGE1, and X1 is a bivalent connecting bridge, and their use for treating impotence.
SUMMARY OF THE INVENTION
It is an object of the present invention to provide new derivatives of prostaglandins able not only to eliminate or at least reduce the side effects associated with these compounds, but also to possess an improved pharmacological activity. It has been surprisingly found that prostaglandin nitroderivatives have a significantly improved overall profile as compared to native prostaglandins both in terms of wider pharmacological activity and enhanced tolerability. In particular, it has been recognized that the prostaglandin nitroderivatives of the present invention can be employed for treating glaucoma and ocular hypertension. The compounds of the present invention are indicated for the reduction of intraocular pressure in patients with open-angle glaucoma or with chronic angle-closure glaucoma who underwent peripheral iridotomy or laser iridoplasty.


DETAILED DESCRIPTION OF THE INVENTION
An object of the present invention is, therefore, prostaglandin nitroderivatives of general formula (I) and pharmaceutically acceptable salts or stereoisomers thereof

R—X—Y—ONO2  (I)

wherein R is the prostaglandin residue of formula (II):





wherein

    the symbol  represents a single bond or a double bond;
    L is selected from the following groups:






X is —O—, —S— or —NH—;

    Y is a bivalent radical having the following meaning:
    a)
    
        straight or branched C1-C20 alkylene, preferably C1-C10, being optionally substituted with one or more of the substituents selected from the group consisting of: halogen atoms, hydroxy, —ONO2 or T, wherein T is
        —OC(O)(C1-C10 alkyl)-ONO2 or —O(C1-C10 alkyl)-ONO2;
        cycloalkylene with 5 to 7 carbon atoms into cycloalkylene ring, the ring being optionally substituted with side chains T1, wherein T1 is straight or branched C1-C10 alkyl, preferably CH3;
    
    






wherein n is an integer from 0 to 20, and n1 is an integer from 1 to 20;





wherein

    X1=—OCO— or —COO— and R2 is H or CH3;
    Z is —(CH)n1— or the bivalent radical defined above under b) n1 is as defined above and n2 is an integer from 0 to 2;






wherein:

    Y1 is —CH2—CH2—(CH2)n2—; or —CH═CH—(CH2)n2—;
    Z is —(CH)n1— or the bivalent radical defined above under b)
    n1, n2, R2 and X1 are as defined above;






wherein:

    n1 and R2 are as defined above, R3 is H or —COCH3;
    with the proviso that when Y is selected from the bivalent radicals mentioned under b)-f), the terminal —ONO2 group is bound to —(CH2)n1;






wherein X2 is —O— or —S—, n3 is an integer from 1 to 6, preferably from 1 to 4, R2 is as defined above;





wherein:

    n4 is an integer from 0 to 10;
    n5 is an integer from 1 to 10;
    R4, R5, R6, R7 are the same or different, and are H or straight or branched C1-C4 alkyl, preferably R4, R5, R6, R7 are H;
    wherein the —ONO2 group is linked to






wherein n5 is as defined above;

    Y2 is an heterocyclic saturated, unsaturated or aromatic 5 or 6 members ring, containing one or more heteroatoms selected from nitrogen, oxygen, sulfur,
    and is selected from









The term “C1-C20 alkylene” as used herein refers to branched or straight chain C1-C20 hydrocarbon, preferably having from 1 to 10 carbon atoms such as methylene, ethylene, propylene, isopropylene, n-butylene, pentylene, n-hexylene and the like.
The term “C1-C10 alkyl” as used herein refers to branched or straight chain alkyl groups comprising one to ten carbon atoms, including methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, t-butyl, pentyl, hexyl, octyl and the like.
The term “cycloalkylene” as used herein refers to ring having from 5 to 7 carbon atoms including, but not limited to, cyclopentylene, cyclohexylene optionally substituted with side chains such as straight or branched (C1-C10)-alkyl, preferably CH3.
The term “heterocyclic” as used herein refers to saturated, unsaturated or aromatic 5 or 6 members ring, containing one or more heteroatoms selected from nitrogen, oxygen, sulphur, such as for example pyridine, pyrazine, pyrimidine, pyrrolidine, morpholine, imidazole and the like.
As stated above, the invention includes also the pharmaceutically acceptable salts of the compounds of formula (I) and stereoisomers thereof.
Examples of pharmaceutically acceptable salts are either those with inorganic bases, such as sodium, potassium, calcium and aluminium hydroxides, or with organic bases, such as lysine, arginine, triethylamine, dibenzylamine, piperidine and other acceptable organic amines.
The compounds according to the present invention, when they contain in the molecule one salifiable nitrogen atom, can be transformed into the corresponding salts by reaction in an organic solvent such as acetonitrile, tetrahydrofuran with the corresponding organic or inorganic acids.
Examples of organic acids are: oxalic, tartaric, maleic, succinic, citric acids. Examples of inorganic acids are: nitric, hydrochloric, sulphuric, phosphoric acids. Salts with nitric acid are preferred.
The compounds of the invention which have one or more asymmetric carbon atoms can exist as optically pure enantiomers, pure diastereomers, enantiomers mixtures, diastereomers mixtures, enantiomer racemic mixtures, racemates or racemate mixtures.
Within the scope of the invention are also all the possible isomers, stereoisomers and their mixtures of the compounds of formula (I), including mixtures enriched in a particular isomer.
Preferred compounds of formula (I) are those wherein R, L, X are as defined in claim 1 and Y is a bivalent radical having the following meaning:

    
    
        a)
        straight or branched C1-C20 alkylene, being optionally substituted with one or more of the substituents selected from the group consisting of: halogen atoms, hydroxy, —ONO2 or T, wherein T is —OC(O)(C1-C10 alkyl)-ONO2 or —O(C1-C10 alkyl)-ONO2;
        cycloalkylene with 5 to 7 carbon atoms into cycloalkylene ring, the ring being optionally substituted with side chains T1, wherein T1 is straight or branched C1-C10 alkyl;
    
    






wherein n is an integer from 0 to 20, and n1 is an integer from 1 to 20;





wherein:

    n1 is as defined above and n2 is an integer from 0 to 2;
    X1=—OCO— or —COO— and R2 is H or CH3;






wherein:

    n1, n2, R2 and X1 are as defined above;
    Y1 is —CH2—CH2— or —CH═CH—(CH2)n2—;






wherein:

    n1 and R2 are as defined above, R3 is H or —COCH3;
    with the proviso that when Y is selected from the bivalent radicals mentioned under b)-f), the —ONO2 group is bound to —(CH2)n1;






wherein X2 is —O— or —S—, n3 is an integer from 1 to 6 and R2 is as defined above;





wherein:

    n4 is an integer from 0 to 10;
    n5 is an integer from 1 to 10;
    R4, R5, R6, R7 are the same or different, and are H or straight or branched C1-C4 alkyl;

wherein the —ONO2 group is linked to







wherein n5 is as defined above;

    Y2 is an heterocyclic saturated, unsaturated or aromatic 5 or 6 members ring, containing one or more heteroatoms selected from nitrogen, oxygen, sulfur,
    and is selected from









Preferred compounds of formula (I) are those wherein

    the prostaglandin residue R is selected from the group consisting of latanoprost, travoprost, unoprostone and cloprostenol, preferably R is latanoprost.
    X is preferably —O— or —S—;


A preferred group of compounds of general formula (I) are those wherein Y is a bivalent radical having the following meaning:

    
    
        a)
        straight or branched C2-C6 alkylene, being optionally substituted with —ONO2 or T, wherein T is as above defined;
    
    






wherein n is an integer from 0 to 5, and n1 is an integer from 1 to 5;





wherein X2 is —O— or —S—, n3 is 1, R2 is as defined above.

Most preferred meanings of Y are:

    a) branched C2-C6 alkylene or straight or branched C2-C6 alkylene being optionally substituted with —ONO2 or T, wherein T is as defined in claim 1;






wherein n is 0, and n1 is 1.





wherein X2 is —O— or —S—, n3 is 1, R2 is hydrogen;

Another preferred group of compounds of general formula (I) are those wherein Y is a bivalent radical having the following meaning:




wherein

    X1=—OCO— or —COO— and R2 is H or CH3;
    Z is —(CH)n1— or the bivalent radical defined above under b) wherein n is an integer from 0 to 5;
    n1 is an integer from 1 to 5 and n2 is an integer from 0 to 2;






wherein:

    Y1 is —CH2—CH2—(CH2)n2—; or —CH═CH—(CH2)2—;
    Z is —(CH)n1— or the bivalent radical defined above under b) n1, n2, R2 and X1 are as defined above;






wherein:

    n1 and R2 are as defined above, R3 is H or COCH3;
    with the proviso that when Y is selected from the bivalent radicals mentioned under b)-f), the —ONO2 group is bound to —(CH2)n1;






wherein:

    n4 is an integer from 0 to 3;
    n5 is an integer from 1 to 3;
    R4, R5, R6, R7 are the same and are H;
    and wherein the —ONO2 group is linked to






    Y2 is a 6 member saturated, unsaturated or aromatic heterocyclic ring, containing one or two atoms of nitrogen and selected for example from






The following are preferred compounds according to the present invention:














































As mentioned above, objects of the present invention are also pharmaceutical compositions containing at least a compound of the present invention of formula (I) together with non toxic adjuvants and/or carriers usually employed in the pharmaceutical field.
The preferred route of administration is topical.
The compounds of the present invention can be administered as solutions, suspensions or emulsions (dispersions) in an ophthalmically acceptable vehicle. The term “ophthalmically acceptable vehicle” as used herein refers to any substance or combination of substances which are non-reactive with the compounds and suitable for administration to patient.
Preferred are aqueous vehicles suitable for topical application to the patient's eyes.
Other ingredients which may be desirable to use in the ophthalmic compositions of the present invention include antimicrobials, preservatives, co-solvents, surfactants and viscosity building agents.
The invention also relates to a method for treating glaucoma or ocular hypertension, said method consisting in contacting an effective intraocular pressure reducing amount of a composition with the eye in order to reduce eye pressure and to maintain said pressure on a reduced level.
The doses of prostaglandin nitroderivatives can be determined by standard clinical techniques and are in the same range or less than those described for the corresponding underivatized, commercially available prostaglandin compounds as reported in the: Physician's Desk Reference, Medical Economics Company, Inc., Oradell, N.J., 58th Ed., 2004; The pharmacological basis of therapeutics, Goodman and Gilman, J. G. Hardman, L. e. Limbird, Tenth Ed.
The compositions contain 0.1-0.30 μg, especially 1-10 μg, per application of the active compound.
The treatment may be advantageously carried out in that one drop of the composition, corresponding to about 30 μl, is administered about 1 to 2 times per day to the patient's eye.
It is further contemplated that the compounds of the present invention can be used with other medicaments known to be useful in the treatment of glaucoma or ocular hypertension, either separately or in combination. For example the compounds of the present invention can be combined with (i) beta-blockers, such as timolol, betaxolol, levobunolol and the like (see U.S. Pat. No. 4,952,581); (ii) carbonic anhydrase inhibitors, such as brinzolamide; (iii) adrenergic agonists including clonidine derivatives, such as apraclonidine or brimonidine (see U.S. Pat. No. 5,811,443. Also contemplated is the combination with nitrooxy derivatives of the above reported compounds, for example nitrooxy derivatives of beta-blockers such as those described in U.S. Pat. No. 6,242,432.
The compounds of the present invention can be synthesized as follows.
Synthesis Procedure
The compounds of general formula (I) as above defined, can be obtained:
i) by reacting a compound of formula (III)




wherein

    L is as above defined; P is H or a hydroxylic protecting group such as silyl ethers, such as trimethylsilyl, tert-butyl-dimethylsilyl or acetyl and those described in T. W. Greene “Protective groups in organic synthesis”, Harvard University Press, 1980, 2nd edition, p. 14-118; W is —OH, Cl, or —OC(O)R1 wherein R1 is a linear or branched C1-C5 alkyl; with a compound of formula (IV) Z-Y-Q wherein Y is as above defined, Z is HX or Z1, being X as above defined and Z1 selected from the group consisting of: chlorine, bromine, iodine, mesyl, tosyl; Q is —ONO2 or Z1 and


ii) when Q is Z1, by converting the compound obtained in the step i) into nitro derivative by reaction with a nitrate source such as silver nitrate, lithium nitrate, sodium nitrate, potassium nitrate, magnesium nitrate, calcium nitrate, iron nitrate, zinc nitrate or tetraalkylammonium nitrate (wherein alkyl is C1-C10 alkyl) in a suitable organic solvent such as acetonitrile, tetrahydrofurane, methyl ethyl ketone, ethyl acetate, DMF, the reaction is carried out, in the dark, at a temperature from room temperature to the boiling temperature of the solvent. Preferred nitrate source is silver nitrate and
iii) optionally deprotecting the compounds obtained in step i) or ii) as described in T. W. Greene “Protective groups in organic synthesis”, Harvard University Press, 1980, 2nd edition, p. 68-86. Fluoride ion is the preferred method for removing silyl ether protecting group.

    
    
        The reaction of a compound of formula (III) wherein W=—OH, P and X1 are as above defined, with a compound of formula (IV) wherein Y and Q are as above defined, Z is HX may be carried out in presence of a dehydrating agent as dicyclohexylcarbodiimide (DCC) or N′-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDAC) and a catalyst, such as N,N-dimethylamino pyridine (DMAP). The reaction is carried out in an inert organic solvent dry such as N,N′-dimethylformamide, tetrahydrofuran, benzene, toluene, dioxane, a polyhalogenated aliphatic hydrocarbon at a temperature from −20° C. and 40° C. The reaction is completed within a time range from 30 minutes to 36 hours.
        The compounds of formula (III) wherein W=—OH and P=H are commercially available;
        The compounds of formula (III) wherein W=—OH and P is a hydroxylic protecting group may be prepared from the corresponding compounds wherein P=H as well known in the art, for example as described in T. W. Greene “Protective groups in organic synthesis”, Harvard University Press, 1980, 2nd edition, p. 14-118.
        The reaction of a compound of formula (III) wherein W=—OC(O)R1 wherein R1 is as above defined and P=H or a hydroxylic protecting group, with a compound of formula (IV) wherein Y is as above defined, Z is —OH and Q is —ONO2 may be carried out in presence of a catalyst, such as N,N-dimethylamino pyridine (DMAP). The reaction is carried out in an inert organic solvent such as N,N′-dimethylformamide, tetrahydrofuran, benzene, toluene, dioxane, a polyhalogenated aliphatic hydrocarbon at a temperature from −20° C. and 40° C. The reaction is completed within a time range from 30 minutes to 36 hours.
        The compounds of formula (III) wherein W=—OC(O)R1 and P=H may be obtained from the corresponding acids wherein W=—OH by reaction with a chloroformate such as isobutylchloroformate, ethylchloroformate in presence of a non-nucleophilic base such as triethylamine in an inert organic solvent such as N,N′-dimethylformamide, tetrahydrofuran, a polyhalogenated aliphatic hydrocarbon at a temperature from −20° C. and 40° C. The reaction is completed within a time range from 1 to 8 hours.
        The reaction of a compound of formula (III) wherein W=—OH and P=H, with a compound of formula (IV) wherein Y is as above defined, Z is Z1 and Q is —ONO2 may be carried out in presence of a organic base such as 1,8-diazabiciclo[5.4.0]undec-7-ene (DBU), N,N-diisopropylethylamine, diisopropylamine or inorganic base such as alkaline-earth metal carbonate or hydroxide, potassium carbonate, cesium carbonate, in an inert organic solvent such as N,N′-dimethylformamide, tetrahydrofuran, acetone, methyl ethyl ketone, acetonitrile, a polyhalogenated aliphatic hydrocarbon at a temperature from −20° C. and 40° C., preferably from 5° C. to 25° C. The reaction is completed within a time range from 1 to 8 hours. When Z1 is chosen among chlorine or bromine the reaction is carried out in presence an iodine compound such as KI.
        The reaction of a compound of formula (III) wherein W=Cl and P is as above defined, with a compound of formula (IV) wherein Y is as above defined, Z is —OH and Q is —ONO2 may be carried out in presence of a of a organic base such as N,N-dimethylamino pyridine (DMAP), triethylamine, pyridine. The reaction is carried out in an inert organic solvent such as N,N′-dimethylformamide, tetrahydrofuran, benzene, toluene, dioxane, a polyhalogenated aliphatic hydrocarbon at a temperature from −20° C. and 40° C. The reaction is completed within a time range from 30 minutes to 36 hours.
        The compounds of formula (III) wherein W=Cl may be obtained from the corresponding acids wherein W=—OH by reaction with a thionyl or oxalyl chloride, halides of PIII or PV in solvents inert such as toluene, chloroform, DMF.
        The compounds of formula HO—Y—ONO2, wherein Y is as above defined can be obtained as follows. The corresponding diol derivative, commercially available, or synthesized by well known reactions, is converted in HO—Y-Z1, wherein Z1 is as above defined, by well known reactions, for example by reaction with thionyl or oxalyl chloride, halides of PIII or PV, mesyl chloride, tosyl chloride in solvents inert such as toluene, chloroform, DMF, etc. The conversion to the nitro derivative is carried out as above described. Alternatively the diol derivative can be nitrated by reaction with nitric acid and acetic anhydride in a temperature range from −50° C. to 0° C. according to methods well known in the literature.
        The compounds of formula Z1-Y—ONO2, wherein Y and Z1 are as above defined can be obtained from the halogen derivative Z1-Y-Hal, commercially available or synthesized according to methods well known in the literature, by conversion to the nitro derivative as above described.
        The compounds of formula H—X—Y-Z1, wherein X, Y and Z1 are as above defined can be obtained from the hydroxyl derivative H—X—Y—OH, commercially available or synthesized according to methods well known in the literature, by well known reactions, for example by reaction with thionyl or oxalyl chloride, halides of PIII or PV, mesyl chloride, tosyl chloride in solvents inert such as toluene, chloroform, DMF, etc.
    
    


The following examples are to further illustrate the invention without limiting it.
EXAMPLE 1
Synthesis of [1R-[1α(Z),2α(R*),3α,5α]]-7-[3,5-dihydroxy-2-(3-hydroxy-5-phenylpentyl)cyclopentyl]-5-heptenoic acid 4-(nitrooxy)butyl ester (compound 1)




II EXPERIMENTAL
II.1 Preparation of 4-bromobutanol
Tetrahydrofuran (12.5 g-173 mmol) was charged under nitrogen in a reactor cooled to 5-10° C. Hydrogen bromide (7.0 g-86.5 mmol) was then added slowly and the reaction medium was stirred over a period of 4.5 hours at 5-10° C. The mixture was diluted with 22.5 g of cold water and the pH of this solution was adjusted to pH=5-7 by adding 27.65% sodium hydroxide (2.0 g) keeping the temperature at 5-10° C. The solution was then extracted twice with dichloromethane (13.25 g). The combined organic phases were washed with 25% brine (7.5 g), adjusted to pH=6-7 with 27.65% sodium hydroxide and dried over magnesium sulfate. Dichloromethane was distilled off and crude 4-bromobutanol (10.3 g-66.9 mmol) was obtained in a yield of about 77%.
II.2 Preparation of 4-bromobutyl nitrate
In reactor cooled to −5 to 5° C., nitric acid fuming (8.5 g-135 mmol) was slowly added to a solution of 98% sulfuric acid (13.0 g-130 mmol) in dichloromethane (18.0 g-212 mmol). 4-bromobutanol (10.2 g-66.6 mmol) was then added to this mixture and the reaction medium was stirred at −5 to 5° C. over a period of 2-5 hours. The mixture was poured into cold water (110 g) keeping the temperature between −5° C. and 3° C. After decantation, the upper aqueous phase was extracted with dichloromethane and the combined organic phases were washed with water, adjusted to pH=6-7 by addition of 27.65% sodium hydroxide, washed with brine and dried over magnesium sulfate. Dichloromethane was distilled off under vacuum and crude 4-bromobutyl nitrate (12.7 g-64.1 mmol) was recovered in a yield of about 96%.
II.3 Preparation of [1R-[1α(Z),2β(R*),3α,5α]]-7-[3,5-dihydroxy-2-(3-hydroxy-5-phenylpentyl)cyclopentyl]-5-heptenoic acid 4-(nitrooxy)butyl ester
Latanoprost acid (97.7%, S-isomer <1%) (213 mg, 0.54 mmol) was dissolved in 5.0 g anhydrous DMF. K2CO3 (206 mg, 1.49 mmol), KI (77 mg, 0.46 mmol) and 4-bromobutylnitrate (805 mg, 25% w/w in methylene chloride, 1.02 mmol) were added. The reaction mixture was heated and stirred on a rotary evaporator at 45-50° C.
After 1.5 hour, TLC (Si, CH2Cl2—MeOH, 5%) showed no starting acid.
The reaction mixture was diluted with 100 ml ethyl acetate, washed with brine (3×50 ml), dried over MgSO4 and evaporated to give yellowish oil (420 mg).
1H NMR/13C NMR showed target molecule as a major product together with some starting 4-bromobutylnitrate and DMF.
HPLC showed no starting acid. Residual solvent, 4-bromobutylnitrate and target ester were the main peaks. Butylnitrate ester showed similar UV spectrum as latanoprost and relative retention time was as expected.
Instrument: Bruker 300 MHz
Solvent: CDCl3 
1H-NMR (CDCl3) δ: 7.29-7.19 (5H, m, Ar); 5.45 (1H, m, CH═CH); 5.38 (1H, m, CH═CH); 4.48 (2H, t, CH2—ONO2); 4.18 (1H, m, CH—OH); 4.10 (2H, t, COOCH2); 3.95 (1H, m, CH—OH); 3.68 (1H, m, CH—OH); 2.87-2.60 (2H, m); 2.35 (2H, t); 2.25 (2H, m); 2.13 (2H, m); 1.90-1.35 (16H, m).
13C-NMR (CDCl3) ppm: 173.94 (C═O); 142.14; 129.55 (C5); 129.50 (C6); 128.50; 125.93 78.80 (C11); 74.50 (C9); 72.70 (C—ONO2); 71.39 (C15); 63.57; 52.99 (C12); 51.99 (C8); 41.30 (C10); 39.16 (C16); 33.66; 32.21; 29.73; 27.04; 26.70; 25.04; 24.91; 23.72; 15.37.
EXAMPLE 2
Synthesis of [1R-[1α(Z),2β(R*),3α,5α]]-7-[3,5-dihydroxy-2-(3-hydroxy-5-phenylpentyl)cyclopentyl]-5-heptenoicacid[2-methoxy-4-[2-propenoyloxy(4-nitrooxybutyl)]]phenyl ester (compound 11)
A) Preparation of Ferulic acid 4-(bromo)butyl ester
To a solution of ferulic acid (1 g, 5.15 mmol) in tetrahydrofurane (40 ml), triphenylphosphine (2.7 g, 10.3 mmol) and tetrabromomethane (3.41 g, 10.3 mmol) were added. The mixture was stirred at room temperature for 4 hours. The mixture was filtered and the solvent was evaporated under vacuum. The crude residue was purified by silica gel chromatography, eluent n-hexane/ethyl acetate 7/3. The product (0.77 g) was obtained as a yellow solid. (Yield 46%)
M.p.=83-88° C.
B) Preparation of Ferulic acid 4-(nitrooxy)butyl ester
A solution of compound A (0.8 g, 2.43 mmol) and silver nitrate (1.2 g, 7.29 mmol) in acetonitrile (50 ml) was stirred at 40° C., in the dark, for 16 hours. The precipitate (silver salts) was filtered off and the solvent was evaporated under vacuum. The residue was purified by flash chromatography, eluent n-hexane/ethyl acetate 75/25. The product (0.4 g) was obtained as white powder (yield 53%)
M.p.=63-64° C.
C) Preparation of[1R-[1α(Z),2β(R*),3α,5α]]-7-[3,5-dihydroxy-2-(3-hydroxy-5-phenylpentyl)cyclopentyl]-5-heptenoic acid [2-methoxy-4-[2-propenoyloxy(4-nitrooxybutyl)]]phenyl ester
To a solution of latanoprost acid (0.2 g, 0.51 mmol) in dry tetrahydrofuran (10 ml), in atmosphere inert, ferulic acid 4-(nitrooxy)butyl ester (0.32 g, 1.02 mmol) and DMAP (cat. amount) were added. The reaction was cooled at 0° C. and EDAC (0.14 g, 0.76 mmol) was added. The reaction was stirred at room temperature for 24 hours. The solution was treated with water and chloroform, the organic layers were anidrified with sodium sulfate and concentrated under reduced pressure. The residue was purified by flash chromatography, eluent n-hexane/ethyl acetate 3/7. The product (0,2g) was obtained.
1H-NMR (CDCl3) δ: 7.55 (1H, d, CH═CHCO); 7.30-7.03 (8H, m, Ar); 6.35 (1H, d, CH═CHCO); 5.48 (2H, m, CH═CH); 4.52 (2H, t, CH2—ONO2); 4.25 (2H, t, COO—CH2); 4.17 (1H, m, CH—OH); 3.95 (1H, m, CH—OH); 3.85 (3H, s, OCH3); 3.65 (1H, m, CH—OH); 2.75 (2H, m); 2.61 (2H, t); 2.48-2.20 (5H, m); 1.9-1.20 (19H, m).
13C-NMR (CDCl3): ppm: 171.62 (C═O); 166.69 (C═O); 151.40; 144.36; 142.04; 141.55; 133.21; 129.62; 129.41; 128.40; 125.85, 123.27; 121.27; 117.96; 111.32; 78.81; 74.84; 72.64 (C—ONO2); 71.32; 63.61; 55.94; 52.99; 51.91; 42.54; 39.08; 35.79; 33.37; 32.12; 29.68; 27.03; 26.53; 25.09; 24.90; 23.73.
EXAMPLE 3
Synthesis of [1R-[1α(Z),2β(R*),3α,5α]]-7-[3,5-dihydroxy-2-(3-hydroxy-5-phenylpentyl)cyclopentyl]-5-heptenoic acid 3-(nitrooxymethyl)phenyl ester (compound 4)
1. Preparation of 3-[(Bromo)methyl]phenol
3-[(Hydroxy)methyl]phenol was dissolved in acetonitrile (300 ml) and dichloromethane (900 ml) and the resulting mixture was poured in the flask kept under argon; magnetic stirring was set on. The solution was then cooled with an ice bath and carbon tetrabromide and triphenilphosphine were added. The latter was added in small portions in order to maintain the temperature at ca. 2-3° C.
The solution was stirred for 1 hour at 2-3° C. and then for an additional hour at room temperature.
After this period the reaction conversion (checked by TLC, using EtOAc/Petroleum ether 3/7as the eluent) was complete. The obtained mixture was evaporated under reduce pressure and 500 ml of petroleum ether and 500 ml of EtOAc were added to the resulting yellow thick oil in a 2 l round flask. A pitchy solid was formed. The mixture was kept under stirring at room temperature overnight and subsequently filtered and concentrated under reduce pressure, furnishing ca. 50 g of an oily residue. The oil was purified by flash chromatography over 600 g of silica gel, using EtOAc/Petroleum ether 2/8as the eluent. Further purification was achieved by crystallising the resulting bromide from petroleum ether. A white solid was obtained (24 g, 64%).
Analysis
TLC: (EtOAc/Petroleum ether 3/7) Rf=0.4
HPLC purity: >98%
FT-IR (KBr, cm−1): 3252, 1589, 1479, 1392, 1270, 1208, 1155, 952, 880, 791, 741, 686.
2. Preparation of 3-[(Nitrooxy)methyl]phenol
3-[(Bromo)methyl]phenol was dissolved in 30 ml of acetonitrile and poured in the flask, kept far from light sources at 0-5° C. under argon; magnetic stirring was set on. Silver nitrate was then added under these conditions, maintaining the temperature under 5° C. The reaction course was followed by TLC (EtOAc/Petroleum ether 3/7as the eluent). After 4 hours and 30 minutes the conversion was complete. The reaction mixture was then filtered, the precipitated solid was washed with Et2O and the filtrate was separated in two batches. The first batch (15 ml) was kept under argon and in acetonitrile solution at −20° C. The second batch (15 ml) was worked-up as follows. The acetonitrile solution was concentrated under reduce pressure and the resulting oil was dissolved in dichloromethane (15 ml) and washed with brine (15 ml). The organic phase was separated and the aqueous phase was extracted twice with dichloromethane (2×25 ml). The combined organic phases were then dried over MgSO4, filtered and evaporated. The residue was purified by flash chromatography over 40 g of silica gel using EtOAc/Petroleum ether 2/8 as the eluent. The nitrate was obtained as an oil (0.6 g, 67%).
Analysis
TLC: (EtOAc/Petroleum ether 3/7) Rf=0.35
HPLC purity: >98%
MS (ESI−): 168 (M+−1)
FT-IR (neat oil, cm−1): 3365, 1632, 1599, 1459, 1282, 1160, 923, 867, 793, 757.
1H NMR (CDCl3, 300 MHz) δ 5.31 (2H, s), 5.45 (1H, br s), 6.78-6.84 (2H, m), 6.87-6.92 (1H, m), 7.17-7.24 (1H, m).
3. Preparation of [1R-[1α(Z),2β(R*),3α,5α]]-7-[3,5-dihydroxy-2-(3-hydroxy-5-phenylpentyl)cyclopentyl]-5-heptenoic acid 3-(nitrooxymethyl)phenyl ester
To a solution of latanoprost acid (0.11 g, 0.28 mmol) in chloroform (20 ml), in atmosphere inert, 3-(nitrooxymethyl)phenol (0.01 g, 0.56 mmol) and DMAP (cat. amount) were added. The reaction was cooled at 0° C. and EDAC (0.08 g, 0.42 mmol) was added. The reaction was stirred at room temperature for 24 hours. The solution was treated with water, the organic layers were anidrified with sodium sulfate and concentrated under reduced pressure. The residue was purified by flash chromatography, eluent n-hexane/ethyl acetate 3/7. The product (0,1 g) was obtained.
1H-NMR (CDCl3) δ: 7.41 (1H, t, Ar); 7.31-7.10 (8H, m, Ar); 5.48 (2H, m, CH═CH); 5.43 (2H, s, CH2—ONO2); 4.16 (1H, m, CH—OH); 3.95 (1H, m, CH—OH); 3.65 (1H, m, CH—OH); 2.75 (2H, m); 2.61 (2H, t); 2.48-2.20 (5H, m); 1.9-1.20 (11H, m).
EXAMPLE 4
Synthesis of [1R-[1α(Z),2β(R*),3α,5α]]-7-[3,5-dihydroxy-2-(3-hydroxy-5-phenylpentyl)cyclopentyl]-5-heptenoic acid 4-(nitrooxymethyl)benzyl ester (compound 9)


    A) [1R-[1α(Z),2β(R*),3α,5α]]-7-[3,5-dihydroxy-2-(3-hydroxy-5-phenylpentyl)cyclopentyl]-5-heptenoic acid 4-(bromomethyl)benzyl ester


To a solution of latanoprost acid (0.5 g, 1.2 mmol) in chloroform (50 ml), in inert atmosphere, 4-(bromomethyl)benzyl alcohol (0.4 g, 1.92 mmol) and DMAP (cat. amount) were added. The reaction was cooled at 0° C. and EDAC (0.37 g, 1.92 mmol) was added. The reaction was stirred at room temperature for 5 hours. The solution was treated with water, the organic layers were anidrified with sodium sulfate and concentrated under reduced pressure. The residue was purified by flash chromatography, eluent n-hexane/ethyl acetate 3/7. The product (0.47 g) was obtained.

    B) [1R-[1α(Z),2β(R*),3α,5α]]-7-[3,5-dihydroxy-2-(3-hydroxy-5-phenylpentyl)cyclopentyl]-5-heptenoic acid 4-(nitrooxymethyl)benzyl ester


A solution of compound A (0.4 g, 0.7 mmol) and silver nitrate (0.23 g, 1.4 mmol) in acetonitrile (50 ml) was stirred at 40° C., in the dark, for 4 hours. The precipitated (silver salts) was filtered off and the solvent was evaporated under vacuum. The residue was purified by flash chromatography, eluent n-hexane/ethyl acetate 7/3. The product (0.15 g) was obtained as oil.
1H-NMR δ: 7.39 (4H, s, Ar); 7.31-7.17 (5H, m, Ar); 5.44 (2H, m, CH═CH); 5.42 (2H, s, CH2—ONO2); 5.30 (2H, s, O—CH9—Ar); 4.15 (1H, m, CH—OH); 3.95 (1H, m, CH—OH); 3.67 (1H, m, CH—OH); 2.75 (2H, m); 2.41 (2H, t); 2.48-1.20 (16H, m).
EXAMPLE 5
Synthesis of [1R-[1α(Z),2β(R*),3α,5α]]-7-[3,5-dihydroxy-2-(3-hydroxy-5-phenylpentyl)cyclopentyl]-5-heptenoic acid 3-(nitrooxy)propyl ester (compound 78)
The compound is synthesized using the procedure described in EXAMPLE 4 starting from latanoprost acid and 3-bromopropanol.
EXAMPLE 6
Synthesis of [1R-[1α(Z),2β(R*),3α,5α]]-7-[3,5-dihydroxy-2-(3-hydroxy-5-phenylpentyl)cyclopentyl]-5-heptenoic acid 2-(nitrooxy)ethyl ester (compound 77)
The compound is synthesized using the procedure described in EXAMPLE 4 starting from latanoprost acid and 2-bromoethanol.
EXAMPLE 7
Synthesis of [1R-[1α(Z),2β(R*),3α,5α]]-7-[3,5-dihydroxy-2-(3-hydroxy-5-phenylpentyl)cyclopentyl]-5-heptenoic acid 6-(nitrooxy)hexyl ester (compound 79)
The compound is synthesized using the procedure described in EXAMPLE 4 starting from latanoprost acid and 6-bromohexanol.
EXAMPLE 8
Synthesis of [1R-[1α(Z),2β(R*),3α,5]]-7-[3,5-dihydroxy-2-(3-hydroxy-5-phenylpentyl)cyclopentyl]-5-heptenoic acid 2-(nitrooxy)-1-methylethyl ester (compound 80)
The compound is synthesized using the procedure described in EXAMPLE 4 starting from latanoprost acid and 1-bromo-2-propanol.
EXAMPLE 9
Synthesis of [1R-[1α(Z),2β(R*),3α,5α]]-7-[3,5-dihydroxy-2-(3-hydroxy-5-phenylpentyl)cyclopentyl]-5-heptenoic acid 2-(nitrooxy)propyl ester (compound 81)
The compound is synthesized using the procedure described in EXAMPLE 4 starting from latanoprost acid and 2-chloro-1-propanol.
EXAMPLE 10
Synthesis of [1R-[1α(Z),2β(R*),3α,5α]]-7-[3,5-dihydroxy-2-(3-hydroxy-5-phenylpentyl)cyclopentyl]-5-heptenoic acid 2-(nitrooxy)-1-(nitrooxymethyl)ethyl ester (compound 82)
The compound is synthesized using the procedure described in EXAMPLE 4 starting from latanoprost acid and 1,3-dibromo-2-propanol.
EXAMPLE 11
Synthesis of [1R-[1α(Z),2β(R*),3α,5α]]-7-[3,5-dihydroxy-2-(3-hydroxy-5-phenylpentyl)cyclopentyl]-5-heptenoic acid [2-methoxy-4-[2-propenoyloxy(2-nitrooxyethyl)]]phenyl ester (compound 83)
The compound is synthesized using the procedure described in EXAMPLE 2 starting from latanoprost acid and ferulic acid 2-(nitrooxy)ethyl ester.
EXAMPLE 12
Synthesis of [1R-[1α(Z),2β(R*),3α,5α]]-7-[3,5-dihydroxy-2-(3-hydroxy-5-phenylpentyl)cyclopentyl]-5-heptenoic acid 2-methoxy-4-[2-propenoyloxy(3-nitrooxmethylphenyl)]]phenyl ester (compound 84)
The compound is synthesized using the procedure described in EXAMPLE 2 starting from latanoprost acid and ferulic acid 3-(nitrooxymethyl)phenyl ester.
EXAMPLE 13
Synthesis of [1R-[1α(Z),2β(R*),3α,5α]]-7-[3,5-dihydroxy-2-(3-hydroxy-5-phenylpentyl)cyclopentyl]-5-heptenoic acid 2-methoxy-4-[2-propenoyloxy(4-nitrooxmethylbenzyl)]]phenyl ester (compound 85)
The compound is synthesized using the procedure described in EXAMPLE 2 starting from latanoprost acid and ferulic acid 4-(nitrooxymethyl)benzyl ester.
EXAMPLE 14
Synthesis of [1R-[1α(Z),2β(R*),3α,5α]]-7-[3,5-dihydroxy-2-(3-hydroxy-5-phenylpentyl)cyclopentyl]-5-heptenoic acid (4-nitrooxmethyl)phenyl ester (compound 6)
The compound is synthesized using the procedure described in EXAMPLE 4 starting from latanoprost acid 4-(chloromethyl)phenyl ester.
EXAMPLE 15
Synthesis of [1R-[1α(Z),2β(R*),3α,5α]]-7-[3,5-dihydroxy-2-(3-hydroxy-5-phenylpentyl)cyclopentyl]-5-heptenoic acid (3-nitrooxmethyl)benzyl ester (compound 8)
The compound is synthesized using the procedure described in EXAMPLE 4 starting from latanoprost acid 4-(bromomethyl)benzyl ester.
EXAMPLE 16
Preparation of an Ophthalmic Composition Using [1R-[(1α(Z),2α(R*),3α,5α]]-7-[3,5-dihydroxy-2-(3-hydroxy-5-phenylpentyl)cyclopentyl]-5-heptenoic acid 4-(nitrooxy)butyl ester (compound 1)














Ingredient
Amount (mg/ml)



















Compound 1
0.1



Tween 80
5



Benzalkonium chloride
0.2



Buffer
q.s.







Buffer:



NaCl 4.1 mg/ml



NaH2PO4 (anh.) 4.74 mg/ml



NaH2PO4 (monohyd.) 4.6 mg/ml



water for injection qs.






EXAMPLE 17
Evaluation of Nitric Oxide-Mediated Activity
The formation of cyclic guanosine-3′,5′ monophosphate (cGMP) in cells in the eye is involved in the regulation of aqueous humor flow. Thus, elevation of cGMP levels leads to decreased aqueous humor production and reduction of intraocular pressure.
We measured the effects of test drugs on cGMP formation in a well established cell assay.
Undifferentiated pheochromocytoma cells (PC12) were used. The monolayer cells were incubated for 45 min in Hank's Balanced Salt Solution enriched with 10 mM Hepes, 5 mM MgCl2 and 0.05% ascorbic acid at the final pH of 7.4 and containing 100 μM of the phosphodiesterase inhibitor, isomethyl-butyl-xanthine (IBMX), 30 μM of the guanylyl cyclase inhibitor, YC-1, and the test drugs at the appropriate concentration. The reaction was terminated by the removal of the incubating buffer followed by the addition of 50 L of 100% ice-cold ethanol. The plate was then dried under hot air steam and the residue dissolved, extracted and analysed using commercially available cyclic cGMP enzyme immunoassay kit.
The results are reported in Table 1. The concomitant application of different concentrations of the various Latanoprost nitroderivatives (1-50 μM) elicited cGMP accumulation in a concentration-dependent fashion.
These effects were not shared by the parent drug Latanoprost suggesting that such effects are dependent on the release of exogenous NO.







TABLE 1







Potency and Efficacy of Latanoprost and respective nitroderivatives


on cGMP accumulation in rat pheochromocytoma cells.












EC50
Emax



Drugs
(μM)
(% over vehicle)







Latanoprost
Not effective
Not effective



Compound 1 (ex. 1)
2.4
290



Compound 4 (ex. 3)
4.4
450



Compound 11 (ex. 2)
1.5
480







EC50 = effective concentration producing half maximal response



Emax = maximum effect






EXAMPLE 18
Evaluation of the Efficacy of Latanoprost Nitroderivative on Intraocular Pressure
Male NZW rabbits ranging from 3-5 kgs of body weight were used in this study. Briefly, the ability of Latanoprost nitroderivative (compound 4, EXAMPLE 3) at reducing intraocular pressure (IOP) was tested in animals previously treated with intracameral injection of 0.25% carbomer solution installation until after a stable increase of the intraocular pressure was reached. In this particular study, test drugs were administered to one eye with the dosage schedule of 1 drop/eye/day for 5 days a week with a physiologic solution containing 0.005% of control or test compounds. The IOP was monitored 3 h after drug application, two-three times weekly for a total of 4 weeks. This concentration was chosen as it reflects that of latanoprost isopropyl ester currently used in clinic to treat the increase of IOP observed in glaucoma patients. Furthermore, at each visit, about 200 μl of aqueous humor was collected using a 30 gauge needle from both eyes under lidocaine anesthesia for further biochemical evaluation of cGMP, camp and nitrite/nitrate contents.
The installation of 0.25% carbomer solution into the eye resulted in a profound increase of the IOP to about 40 mmHg that remained stable thereafter. However, the administration of the compound 4 (EX. 3) with the dose schedule outline in the method session, decreased the intraocular pressure of these animals of about 50% within 7 days of repeated treatments and over 65% by the end of the study (See Table 2). In contrast, neither Latanoprost acid (data not shown) nor its isopropyl derivative elicited any appreciable change (see Table 2). Given the literature available documenting that Latanoprost is virtually not effective in rabbits, the observed effects are likely to be attributed to the presence of the nitric oxide (NO) moiety onto Latanoprost nitroderivative rather than the parent compound.
Biochemical measurements of cGMP, cAMP and NOx in the intraocular aqueous humor further supported the role of NO at decreasing the IOP of these animals. In fact, as shown in Table 3, the extent of cGMP and NOx increased following the application of the compound 4 (EX. 3) over the 4-week treatment. These effects turn out to be highly specific as the amount of intraocular cAMP remained unaltered in these animals. Latanoprost isopropyl ester did not significantly affect the levels of either cGMP, cAMP or nitrites when given at equimolar doses to that of the respective nitroderivative (see Table 3).







TABLE 2







Reversal of stimuli carbomer-evoked increase in IOP before


(pre-treatment) and after eye-installation of equimolar Latanoprost


isopropyl ester or the respective nitroderivative















IOP
Pre-









mmHg
treatment*
Day 2
Day 7
Day 10
Day 15
Day 17
Day 23
Day 25





Latanoprost
37 ± 2
34 ± 2
33 ± 3
30 ± 1
31 ± 2
30 ± 2
32 ± 2
30 ± 2


isopropyl ester


Compound 4
42 ± 2
31 ± 1
26 ± 1
20 ± 1
18 ± 2
16 ± 1
15 ± 1
14 ± 1


(ex. 3)





*Pre-treatment values correspond to baseline IOP evoked following the intracameral installation of 0.25% carbomer solution.













TABLE 3







Effects of Latanoprost isopropyl ester and the respective nitroderivative


on cGMP, cAMP and NOx content in carbomer-treated rabbits.












IOP
Pre-
I
II
III
IV


mmHg
treatment*
Week
Week
Week
Week





cGMP (fmol/mg prot)







Latanoprost isopropyl
87 ± 6
88 ± 6
98 ± 6
99 ± 6
100 ± 6 


ester Compound 4 (ex. 3)
88 ± 5
102 ± 5 
125 ± 5 
140 ± 5 
160 ± 5 


cAMP (fmol/mg prot)


Latanoprost isopropyl
510 ± 18
550 ± 22
600 ± 30
620 ± 31
625 ± 31


ester Compound 4 (ex. 3)
520 ± 20
600 ± 25
650 ± 31
680 ± 28
660 ± 22


NOx (nmol/mg prot)


Latanoprost isopropyl
16 ± 1
18 ± 2
18 ± 1
19 ± 2
19 ± 2


ester Compound 4 (ex. 3)
17 ± 1
22 ± 2
25 ± 3
26 ± 3
28 ± 3





*Pre-treatment values correspond to baseline IOP evoked following the intracameral installation of 0.25% carbomer solution.

## Claims

1. A pharmaceutical composition comprising a compound of the formula




or a pharmaceutically acceptable salt or stereoisomer thereof, and a pharmaceutically acceptable carrier.

2. A pharmaceutical composition according to claim 1 in a suitable form for topical administration.

3. A pharmaceutical composition according to claim 1, wherein said compound is administered as a solution, suspension or emulsion in an ophthalmically acceptable vehicle.

4. A method for treating glaucoma or ocular hypertension, said method comprising contacting an effective intraocular pressure reducing amount of a pharmaceutical composition according to claim 2 with the eye in order to reduce eye pressure and to maintain said pressure on a reduced level.

5. A method for treating glaucoma or ocular hypertension, said method comprising contacting an effective intraocular pressure reducing amount of a pharmaceutical composition according to claim 3 with the eye in order to reduce eye pressure and to maintain said pressure on a reduced level.