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                            Library & Multimedia
 Recovery of Whole Mitochondrial Genome From Compromised Samples via Multiplex PCR and Massively Parallel Sequencing
NCJ Number
              254295
Journal

*Future Science Oa* Volume: 4 Issue: 9 Dated: 2018
Author(s)
          Maureen P. Hickman;
                        Kelly S. Grisdale;
                        Brittania J Bintz;
                        Erin S. Burnside;
                        Erin K. Henson;
                        Jack ballantyne;
                        Mark R. Wilson
Date Published

2018
Length
              12 pages
Annotation
              This article reports on a project that developed a multiplex PCR method that amplifies the complete mitochondrial genome in a range of forensically relevant samples, including single cells, cremated remains, bone, maggot, and hairs isolated from dust bunnies.
Abstract
              In forensic casework, compromised samples often have limited or degraded nuclear DNA, rendering mitochondrial DNA a more feasible option for forensic DNA analyses. The emergence of massively parallel sequencing (MPS) has enabled the recovery of extensive sequence information from low quantities of DNA. In the current project, MPS yields completed or nearly completed mitochondrial genome coverage for all samples. To confirm concordance between sample types and between sequencing platforms, the project compared sequencing results from hair and buccal swabs from two references. Low initial DNA input into the multiplex PCR allowed for conservation of precious DNA, while MPS maximized recovery of genetic information. (publisher abstract modified)
      
  
          Additional Details
Grant Number(s)
        2013-DN-BX-K014


  
  
        
  Agencies
        NIJ-Sponsored


  
  
        
  Publication Type
        Research (Applied/Empirical)
                                Report (Study/Research)
                                Report (Grant Sponsored)
                                Program/Project Description


  
  
        
  Award
        Assessing Sample Preparation Methods for Emerging DNA Sequencing Technologies in Human Forensic mtDNA Analysis Applications
    
  
  
Date Published: January 1, 2018
    
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                Degraded DNA samples
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