U.S. flag
      An official website of the United States government, Department of Justice.
        Here's how you know
      Here's how you know**Official websites use .gov**
            
            A **.gov** website belongs to an official government organization in the United States.
      **Secure .gov websites use HTTPS**
            
            A **lock** (
            A locked padlock
            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites. Menu

      
        close

            
            
                                              About NIJ
        
                    
  
          About NIJ
      
    
          The NIJ Director
          How NIJ Is Organized
          Mission and Guiding Principles
          Research and Development Process
          Strategic Framework
          Assessments of NIJ
          Annual Reports & Reports to Congress
          Staff Directory
          Funding & Awards
        
                    
  
          Funding & Awards
      
    
          Current Funding
          Forthcoming Funding
          Expired Funding
          Awards: Listing of Funded Projects
          Guidance for Applicants and Awardees
          Fellowship & Student Programs
          Travel Scholarships
          NIJ Challenge Program
          Library & Multimedia
        
                    
  
          Library & Multimedia
      
    
          NIJ Journal
          Podcasts
          Publications Listing
          Multimedia Listing
          Rated Practices on CrimeSolutions
          Rated Programs on CrimeSolutions
          Software & Databases
          Datasets
          Patents
          Topics
        
                    
  
          Topics
      
    
          Article Listing
          Corrections
          Courts
          Crime Prevention
          Crimes
          Drugs and Crime
          Equipment and Technology
          Forensic Sciences
          Youth Justice
          Justice System Reform
          Law Enforcement
          Tribal Crime and Justice
          Victims of Crime
          Events
        
                    
  
          Events
      
    
          NIJ 2024 Research Conference
          Scheduled Events
          On-Demand Events
          Research Meetings & Workshops
          NIJ-Hosted Online Training

                            
                                About
          Contact Us
          Subscribe
          Topics A-Z
                  Share
FacebookXLinkedInRedditPinterestEmail Home
                            Library & Multimedia
 SNP-Based Microarray Technology for Use in Forensic Applications' Final Technical Report
NCJ Number
              223977

Author(s)
          Giulia C. Kennedy
Date Published

January 2008
Length
              43 pages
Annotation
              This project enabled the detection and analysis of forensically relevant single-nucleotide polymorphisms (SNPs), which will enhance and complement the genomic information currently being used for forensic DNA identification.
Abstract
              Using Affymetrix 500K nuclear SNP genotyping arrays, the project demonstrated that preamplification using whole genome amplification (WGA) on low template samples yields successful genotyping for 80-90 percent of the markers on the 500K arrays. These results represent approximately 5-10 percent lower call rates than the non-preamplifier standard assay. Degraded samples prepared by DNase I digestion provide genotype data on many thousands of SNPs in the 500K assay. Using the standard assay, researchers detected mixed samples composed of two, three, or four components. In mixtures with two components, researchers detected as low as a 5-percent contribution from the minor component. The project ran the 500K SNP assay on all samples and analyzed the data using the standard algorithm. The results show that all samples, except for hair shaft (no root) yielded useable genotype data; genders were correctly assigned in all cases; mixtures were detected, and the two components of the mixture were identified; semen and buccal samples yielded high call rates comparable to control samples; vaginal samples had 5-10 percent lower call rates than semen and buccal samples; WGA samples yielded 5-10 percent lower call rates than unamplified genomic DNA; and all ethnicities were correctly identified, including a sample with ancestry from a Taiwanese aborigine. The project focused on developing an accurate, affordable micro array-based forensic DNA analysis assay capable of rapid, simultaneous, SNP genotyping for human identification testing for three forensic sample types. These were characterized by low template single-donor samples; degraded samples; and mixtures that contained two or more DNA sources. 9 tables, 14 figures, and 39 references
      
  
          Additional Details
Grant Number(s)
        2005-DA-BX-K101


  
  
        
  Agencies
        NIJ-Sponsored


  
  
        
  Publication Type
        Report (Study/Research)


  
  
        
  Award
        A SNP-Based Microarray Technology For Use In Forensic Applications
    
  
  
Date Published: January 1, 2008
    
          Downloads
        
                      PDF

Side navigation
  

        

  
  
              NIJ Journal
    
      
            
            
            
      Podcasts
    
      
            
            
            
      Publications Listing
    
      
            
            
            
      Multimedia Listing
    
      
            
            
            
      Rated Practices on CrimeSolutions
    
      
            
            
            
      Rated Programs on CrimeSolutions
    
      
            
            
            
      Software & Databases
    
      
            
            
            
      Datasets
    
      
            
            
            
      PatentsRelated Topics
        
                Criminology
        DNA fingerprinting
        Forensic sciences
        Investigative techniques
        Research



  
Similar Publications
        
                      Aptamer-Based Fentanyl Detection in Biological Fluids
                  Enhancing Foundational Validity of Forensic Findings in Medico-Legal Strangulation Examinations
                  3D Morphology of Blood Stain Can Provide Critical Information Missed In Standard Bloodstain Analysis
 Top

      National Institute of Justice

        
        
                        About NIJ
                    About NIJ
      
    
                  How NIJ Is Organized
      
    
                  NIJ Director
      
    
                  Research Disclaimer
      
    
                  Funding & Awards
                    Funding & Awards Home
      
    
                  Awards Listing
      
    
                  Current Funding
      
    
                  Guidance for Applicants and Awardees
      
    
                  Resources
                    Events
      
    
                  Podcasts
      
    
                  Publications
      
    
                  Topics
      
    
                  Multimedia
      
    
                  Reuse Policy
      
    
                  Contacts
                    Contact NIJ
      
    
                  Staff Directory
      
    
                  Submit a FOIA Request
      
    
                  File a Civil Rights ComplaintFacebook
          
                      X
          
                      YouTube
  

      810 7th St. NW, Washington, D.C. 20531
      
              Secondary Footer link menu
            Accessibility
              Plain Language
              Privacy Policy
              Legal Policies and Disclaimer
              No FEAR Act
              Freedom of Information Act
              USA.gov
              Justice.gov