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                            Library & Multimedia
 A rotationally-driven dynamic solid phase sodium bisulfite conversion disc for forensic epigenetic sample preparation
NCJ Number
              308392
Journal

*Lab on a Chip* Volume: 2024 Issue: 1 Dated: January 2024
Author(s)
          R. Turiello;
                        R. L. Nouwairi;
                        J. Keller;
                        L. L. Cunha;
                        L. M. Dignana;
                        J. P. Landers
Date Published

January 2024
Annotation
              This study introduces a rotationally-driven dynamic solid phase sodium bisulfite conversion disc for forensic epigenetic sample preparation.
Abstract
              Epigenetic analysis requires sodium bisulfite conversion (BSC), a sample preparation method that is time-consuming, labor-intensive, prone to contamination, and characterized by DNA loss and fragmentation. To provide an alternative method for BSC that is more amenable to integration with the forensic DNA workflow, the authors of this study describe a rotationally-driven, microfluidic method for dynamic solid phase-BSC (dSP-BSC) that streamlines the sample preparation process in an automated format, capable of preparing up to four samples in parallel. The method permitted decreased incubation intervals by ∼36% and was assessed for relative DNA recovery and conversion efficiency and compared to gold-standard and enzymatic approaches. (Published Abstract Provided)
      
  
          Additional Details
Grant Number(s)
        2020-R2-CX-0030


  
  
        
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  Publication Type
        Research (Applied/Empirical)


  
  
        
  Award
        Methyl-PrepDisc: A Centrifugal Microfluidic Device for Forensic DNA Methylation Sample Preparation
    
  
  
Date Published: January 1, 2024
    
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