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                            Library & Multimedia
 Routine Mitogenome MPS Analysis from 1 and 5 mm of Rootless Human Hair
NCJ Number
              306070
Journal

*Genes* Volume: 13 Issue: 11 Dated: 2022 Pages: 2144
Author(s)
          Lauren C. Canale;
                        Jennifer A. McElhoe;
                        Gloria Dimick;
                        Katherine M. DeHeer;
                        Jason Beckert;
                        Mitchell M. Holland
Date Published

2022
Annotation
              Using massively parallel sequencing (MPS) of the mitochondrial (mito) genome, the authors studied the impact of hair age (time since collection) and physical characteristics (hair diameter, medullary structure, and length of hair tested) on mtDNA recovery and massively parallel sequencing (MPS) data quality.
Abstract
              Although hair shafts are a common evidence type in forensic cases, they are often excluded from DNA analysis due to their limited DNA quantity and quality. Mitochondrial (mt) DNA sequencing is the method of choice when working with rootless hair shaft fragments due to the elevated copy number of mtDNA and the highly degraded nature of nuclear (n) DNA. In the current study, hair shaft cuttings of 1 and 5 mm from hairs less than five years to 46 years of age from 60 donors were characterized microscopically. Mitogenome sequences were generated using the Promega PowerSeqTM Whole Mito System prototype kit and the Illumina MiSeq instrument. Reportable mitogenome sequences were obtained from all hairs up to 27 years of age (37 donors), with at least 98% of the mitogenome reported for more than 94% of the 74 hair samples analyzed; the minimum reported sequence was 88%. Furthermore, data from the 1- and 5-mm replicates gave concordant haplotypes. As expected, mtDNA yield decreased, mtDNA degradation increased, and mitogenome MPS data quality declined as the age of the hair increased. Hair diameter and medullary structure had minimal impact on yield and data quality. The findings support that MPS is a robust and reliable method for routinely generating mitogenome sequences from 1- and 5-mm hair shaft samples up to 27 years of age, which is of interest to the forensic community, biological anthropologists, and medical geneticists. (Published abstract provided
      
  
          Additional Details
Grant Number(s)
        2019-DU-BX-0045


  
  
        
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        NIJ-Sponsored


  
  
        
  Publication Type
        Research (Applied/Empirical)


  
  
        
  Award
        mtGenome Sequencing of Human Hair Shafts Using an MPS Approach
    
  
  
Date Published: January 1, 2022
    
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      PatentsRelated Topics
        
                Research and Development in Forensic Science for Criminal Justice Purposes
        DNA (Deoxyribonucleic Acid)
        Forensic sciences
        Evidence analysis and processing
        Mitochondrial DNA



  
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