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                            Library & Multimedia
 Massively Parallel DNA Typing by Capillary Array Electrophoresis
NCJ Number
              173057

Author(s)
          G.  F. Sensabaugh
Date Published

January 1997
Length
              85 pages
Annotation
              The use of capillary array electrophoresis (CAE) as an approach to high-throughput DNA typing was examined in a research project that combined the rapid analysis times of capillary electrophoresis with the throughput multiplier of running many capillaries in parallel.
Abstract
              Results revealed the usefulness of CAE for the high-throughput typing of short tandem repeat polymorphisms, both singly and in multiplex sets. The research also demonstrated the usefulness of CAE for high-throughput DNA sequence analysis. Findings also indicated that the use of energy transfer fluorescent labels improves both the sensitivity and the quality of analytical output, both for short tandem repeat typing and for sequence analysis. The researchers also constructed a second-generation CAE unit with four-color detection potential for both short tandem repeat typing and for sequence analysis. Additional research focused on other aspects of short tandem repeat typing, the detection of sample mixtures, and the potential of another genetic typing approach. Figures, tables, appended copies of other reports and papers produced by the same research project, list of other publications, and 61 references
      
  
          Additional Details
Agencies
        NIJ-Sponsored
    
  
  
Date Published: January 1, 1997
    
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