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                            Library & Multimedia
 Rapid Differentiation of Epithelial Cell Types in Aged Biological Samples Using Autofluorescence and Morphological Signatures
NCJ Number
              253142
Journal

*PLoS ONE* Volume: 13 Issue: 5 Dated: 2018
Author(s)
          Emily R. Brocato;
                        Katherine  Philppott;
                        Catherine C. Connon;
                        Christopher J. Ehrhardt
Date Published

2018
Length
              8 pages
Annotation
              Since establishing the tissue source of epithelial cells within a biological sample is an important capability for forensic laboratories, this study used Imaging Flow Cytometry (IFC) to analyze individual cells recovered from buccal, epidermal, and vaginal samples that had been dried between 24 hours and just over 8 weeks.
Abstract
              Measurements that captured the size, shape, and fluorescent properties of cells were collected in an automated manner and then used to build a multivariate statistical framework for differentiating cells based on tissue type. Results showed that epidermal cells could be distinguished from vaginal and buccal cells using a discriminant function analysis of IFC measurements with an average classification accuracy of ~94 percent. Ultimately, cellular measurements such as these, which can be obtained non-destructively, may provide probative information for many types of biological samples and complement results from standard genetic profiling techniques. 4 table, 3 figures, and 21 references (publisher abstract modified)
      
  
          Additional Details
Grant Number(s)
        2013-DN-BX-K033
                                2015-DN-BX-K024


  
  
        
  Agencies
        NIJ-Sponsored


  
  
        
  Publication Type
        Research (Applied/Empirical)
                                Report (Study/Research)
                                Report (Grant Sponsored)
                                Program/Project Description


  
  
        
  Award
        DNA Profiling of Complex Biological Mixtures using HLA-Antibody Probes and Fluorescence Activated Cell Sorting
                                Front End Separation of Compromised Blood Mixtures for Single Source DNA Profiling
    
  
  
Date Published: January 1, 2018
    
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