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                            Library & Multimedia
 Novel cellular signatures for determining time since deposition for trace DNA evidence
NCJ Number
              307010
Journal

*Forensic Science International: Genetics Supplement Series* Volume: 8 Dated: December 2022 Pages: 268-270
Author(s)
          Sarah Ingram;
                        M. Katherine Philpott;
                        Christopher J. Ehrhardt
Date Published

December 2022
Length
              3 pages
Annotation
              This article presents a novel method to determine time since deposition of trace DNA samples presented in forensic cases to answer questions about how and when the DNA was deposited.
Abstract
              With the increase in sensitivity of DNA profiling, questions about how and when the DNA was deposited have become a driving issue in forensic cases. To address this, the authors propose a novel method to determine time since deposition of trace DNA samples based on morphological and autofluorescence properties of individual epithelial cells which can change as the sample ages. To develop this signature, a series of trace DNA samples were generated by contact/handling a substrate and then allowed to age anywhere between one day and more than one year prior to collection. Imaging flow cytometry (IFC) was then used to characterize the morphology and autofluorescence profiles of individual cells within each sample followed by multivariate modelling and predictive classification. (Published Abstract Provided)
      
  
          Additional Details
Grant Number(s)
        2019-DU-BX-0041


  
  
        
  Agencies
        NIJ-Sponsored


  
  
        
  Publication Type
        Research (Applied/Empirical)


  
  
        
  Award
        Determining Time Since Deposition of Epithelial Cell Samples Using Intrinsic Fluorescence Signatures
    
  
  
Date Published: December 1, 2022
    
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        Forensic biology



  
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