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                            Library & Multimedia
 Quadriplex real-time PCR (qPCR) assay for human-canine-feline species identification and nuclear DNA quantification
NCJ Number
              255424
Journal

*Forensic Science International: Genetics* Volume: 6 Dated: May 2012 Pages: e97-e98
Author(s)
          Sreetharan Kanthaswamy;
                        A. Premasuthan
Date Published

May 2012
Length
              0 pages
Annotation
              This letter is an update to a recent publication in the Forensic Science International Genetics journal, “Quantitative real-time PCR (qPCR) assay for human–dog–cat species identification and nuclear DNA quantification”: S. Kanthaswamy, A. Premasuthan, et al.
Abstract
              This triplex qPCR assay quantifies and identifies the presence of as little as 0.4 pg of human, feline and canine DNA in a mixed sample [ [1] ]. The assay did not include an Internal Positive Control (IPC) due to the three-dye limit in the Applied Biosystems 7300 machine. The assay is now modified to include an IPC using the 7500 Fast Real Time PCR system. 1 figure and 3 references (publisher abstract modified)
      
  
          Additional Details
Grant Number(s)
        2008-DN-BX-K288


  
  
        
  Agencies
        NIJ-Sponsored


  
  
        
  Award
        Development of a DNA-Based Multi-Species Identification and Quantification Assay
    
  
  
Date Published: May 1, 2012
    
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      PatentsRelated Topics
        
                DNA (Deoxyribonucleic Acid)
        Forensic sciences
        Canine (K9)



  
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