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                            Library & Multimedia
 Rolling circle amplification: A (random) primer on the enrichment of an infinite linear DNA template
NCJ Number
              308372
Journal

*WIREs Forensic Science* Volume: 2 Dated: 2020 Pages: e1359
Author(s)
          Rachel E. Kieser;
                        Bruce Budowle
Date Published

2020
Annotation
              This paper explores the use of rolling circle amplification (RCA) in DNA typing systems.
Abstract
              Rolling circle amplification (RCA) is a robust enzymatic process in which a circular DNA molecule serves as a template for exponential amplification of molecular targets of interest. Random primers that have bound to DNA sequences across the circularized DNA template initiate copying via the action of a highly processive DNA polymerase. Following a cascade of strand displacement events, long tandem copies of the original template sequence are generated. Because the template molecule is in a circular format, it essentially serves as an infinite linear template. In conjunction with selective enrichment techniques, RCA may prove highly useful in the selectivity and sensitivity of current DNA typing systems. In addition, circularization potentially may allow for amplification of fragmented and low copy number DNA encountered in forensic casework samples that can be subsequently typed. (Published Abstract Provided)
      
  
          Additional Details
Grant Number(s)
        2017-IJ-CX-0010
                                2016-DN-BX-0154


  
  
        
  Agencies
        NIJ-Sponsored


  
  
        
  Publication Type
        Research (Applied/Empirical)


  
  
        
  Award
        Typing Highly Degraded DNA Using Circularized Molecules and Target Enrichment
                                Typing Highly Degraded DNA Using Circularized Molecules and Target Enrichment
    
  
  
Date Published: January 1, 2020
    
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        DNA (Deoxyribonucleic Acid)
        DNA typing
        Trace DNA



  
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