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                            Library & Multimedia
 Quantitative Real-Time PCR (qPCR) Assay for Human-Dog-Cat Species Identification and Nuclear DNA Quantification
NCJ Number
              240637
Journal

*Forensic Science International: Genetics* Volume: 6 Issue: 2 Dated: March 2012 Pages: 290-295
Author(s)
          S. Kanthaswamy;
                        A. Premasuthan;
                        J. Ng;
                        J. Satkoski;
                        V. Goyal
Date Published

March 2012
Length
              6 pages
Annotation
              This paper reports on the development and evaluation of a species-specific (human, dog and cat) nuclear DNA identification assay based on the TaqMan() quantitative real-time PCR (qPCR) technology that can simultaneously detect and measure minute quantities of DNA specific to either humans, dogs and/or cats.
Abstract
              In the United States, human forensic evidence collected from crime scenes is usually comingled with biomaterial of canine and feline origins. Knowledge of the concentration of nuclear DNA extracted from a crime scene biological sample and the species from which the sample originated is essential for DNA profiling. The ability to accurately detect and quantify target DNA in mixed-species samples is crucial when target DNA may be overwhelmed by non-target DNA. The authors have designed and evaluated a species-specific (human, dog and cat) nuclear DNA identification assay based on the TaqMan() quantitative real-time PCR (qPCR) technology that can simultaneously detect and measure minute quantities of DNA specific to either humans, dogs and/or cats. The fluorogenic triplex assay employs primers and hydrolysis probes that target the human TH01 locus as well as the dog and cat Melanocortin 1 Receptor (MC1R) sequences in a species-specific manner. The authors also demonstrate that the assay is a highly sensitive, reliable and robust method for identifying and quantifying mixed-species templates of human-dog-cat origin with as little as 0.4 pg of human and cat nuclear DNA, respectively, and 4.0 pg of dog nuclear DNA. (Published Abstract)
      
  
          Additional Details
Grant Number(s)
        2008-DN-BX-K288


  
  
        
  Agencies
        NIJ-Sponsored


  
  
        
  Publication Type
        Report (Study/Research)


  
  
        
  Award
        Development of a DNA-Based Multi-Species Identification and Quantification Assay
    
  
  
Date Published: March 1, 2012
    
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