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                            Library & Multimedia
 Production of high-fidelity electropherograms results in improved and consistent DNA interpretation: Standardizing the forensic validation process
NCJ Number
              304145
Journal

*Forensic Science International: Genetics* Volume: 31 Dated: 2017 Pages: 160-170
Author(s)
          Kelsey C. Peters;
                        et al
Date Published

2017
Length
              11 pages
Annotation
              This project sought to develop a validation scheme to aid in mitigating the effects of evidence samples in which the loss of genetic information on contributors is associated with sampling and detection effects.
Abstract
              Researchers established a scheme designed to simultaneously improve signal resolution and detection rates without costly large-scale experimental validation studies by applying a combined simulation and experimental approach. Specifically, the project parameterized an in-silico DNA pipeline with experimental data acquired from the laboratory and used this to evaluate multifarious scenarios in a cost-effective manner. Metrics, such as signal1copy-to-noise resolution, false positive, and false negative signal detection rates were used to select tenable laboratory parameters that resulted in high-fidelity signal in the single-copy regime. The project demonstrated that the metrics acquired from simulation were consistent with experimental data obtained from two capillary electrophoresis platforms and various injection parameters. Once good resolution is obtained, analytical thresholds can be determined using detection error tradeoff analysis, if necessary. Decreasing the limit of detection of the forensic process to one copy of DNA is a powerful mechanism by which to increase the information content on minor components of a mixture, which is particularly important for probabilistic system inference. If the forensic pipeline is engineered such that high-fidelity electropherogram signal is obtained, then the likelihood ratio (LR) of a true contributor increases and the probability that the LR of a randomly chosen person is greater than one decreases. This is, potentially, the first step toward standardization of the analytical pipeline across operational laboratories. (publisher abstract modified)
      
  
          Additional Details
Grant Number(s)
        2014-DN-BX-0001
                                2014-DN-BX-K026


  
  
        
  Agencies
        NIJ-Sponsored


  
  
        
  Award
        Establishing Exclusion Criteria and the Significance of Inclusion: Developing Tools for the Interpretation of Complex DNA Mixtures
                                Computational methods for the interpretation of forensic DNA samples
    
  
  
Date Published: January 1, 2017
    
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      PatentsRelated Topics
        
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        Degraded DNA samples
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