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                            Library & Multimedia
 Probabilistic Characterisation of Baseline Noise in STR Profiles
NCJ Number
              250039

Author(s)
          U. L. Monich, K. Duffy
Date Published

November 2015
Length
              16 pages
Annotation
              There are three dominant contributing factors that distort short tandem repeat profile measurements, two of which, stutter and variations in the allelic peak heights, have been described extensively. This study characterizes the remaining component, baseline noise.
Abstract
              A probabilistic characterization of the non-allelic noise peaks is not only inherently useful for statistical inference but is also significant for establishing a detection threshold. This was done by analyzing the data from 643 single person profiles for the Identifiler Plus kit and 303 for the PowerPlex 16 HS kit. This investigation found that although the dye color is a significant factor, it is not sufficient to have a per-dye color description of the noise. Furthermore, this study shows that at a per-locus basis, out of the Gaussian, log-normal, and gamma distribution classes, baseline noise is best described by log-normal distributions and provide a methodology for setting an analytical threshold based on that deduction. In the PowerPlex 16 HS kit, there was evidence of significant stutter at two repeat units shorter than the allelic peak, which has implications for the definition of baseline noise and signal interpretation. In general, the DNA input mass has an influence on the noise distribution. Thus, it is advisable to study noise and, consequently, to infer quantities like the analytical threshold from data with a DNA input mass comparable to the DNA input mass of the samples to be analyzed.  (Publisher abstract modified)
      
  
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        Low-template DNA mixture interpretation: Determining the number of contributors
                                Establishing Exclusion Criteria and the Significance of Inclusion: Developing Tools for the Interpretation of Complex DNA Mixtures
                                Establishing Exclusion Criteria and the Significance of Inclusion for Complex Low-Template DNA Mixtures
    
  
  
Date Published: November 1, 2015
    
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