U.S. flag
      An official website of the United States government, Department of Justice.
        Here's how you know
      Here's how you know**Official websites use .gov**
            
            A **.gov** website belongs to an official government organization in the United States.
      **Secure .gov websites use HTTPS**
            
            A **lock** (
            A locked padlock
            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites. Menu

      
        close

            
            
                                              About NIJ
        
                    
  
          About NIJ
      
    
          The NIJ Director
          How NIJ Is Organized
          Mission and Guiding Principles
          Research and Development Process
          Strategic Framework
          Assessments of NIJ
          Annual Reports & Reports to Congress
          Staff Directory
          Funding & Awards
        
                    
  
          Funding & Awards
      
    
          Current Funding
          Forthcoming Funding
          Expired Funding
          Awards: Listing of Funded Projects
          Guidance for Applicants and Awardees
          Fellowship & Student Programs
          Travel Scholarships
          NIJ Challenge Program
          Library & Multimedia
        
                    
  
          Library & Multimedia
      
    
          NIJ Journal
          Podcasts
          Publications Listing
          Multimedia Listing
          Rated Practices on CrimeSolutions
          Rated Programs on CrimeSolutions
          Software & Databases
          Datasets
          Patents
          Topics
        
                    
  
          Topics
      
    
          Article Listing
          Corrections
          Courts
          Crime Prevention
          Crimes
          Drugs and Crime
          Equipment and Technology
          Forensic Sciences
          Youth Justice
          Justice System Reform
          Law Enforcement
          Tribal Crime and Justice
          Victims of Crime
          Events
        
                    
  
          Events
      
    
          NIJ 2024 Research Conference
          Scheduled Events
          On-Demand Events
          Research Meetings & Workshops
          NIJ-Hosted Online Training

                            
                                About
          Contact Us
          Subscribe
          Topics A-Z
                  Share
FacebookXLinkedInRedditPinterestEmail Home
                            Library & Multimedia
 Proteomic and microRNA Markers of Fly Development: Stable Correlates of Insect Age To Improve Forensic Entomology Casework
NCJ Number
              255339

Author(s)
          Aaron M. Tarone
Date Published

April 2019
Length
              29 pages
Annotation
              This report presents the findings and methodology of a project whose goal was to catalog miRNAs and proteins that are differentially expressed during intrapuparial development of three blow fly species and a flesh fly species of forensic relevance.
Abstract
              Project researchers envision that these stable markers will be used in casework to increase the precision of age estimates of entomological evidence. A description of study methods addresses colony care, the sampling scheme, miRNA extractions and sequencing, sequence analysis, qPCR, protein extractions, DIGE, Western Blot, and the detection of miRNA when traditionally preserved. Data analysis is described. The project has identified just over 200 miRNAs present in the development of three species of flies that have forensic relevance, with flesh fly data forthcoming. Researchers found that expression of these miRNA is differential through intrapuparial development and that statistical analysis of these expression patterns can be used to differentiate the amount of time that has passed since pupariation began. Project findings suggest there are stable and conserved molecular markers for determining the age of pupae and differentiating feeding and post-feeding third instar larvae. In addition, researchers optimized a sex identification assay for three species of carrion flies. Also, significant differences were found in the development of B. plinthopyga on various substrates, which is a step toward increased precision for estimates of this species, which has been found in many indoor cases in Houston, TX.
      
  
          Additional Details
Grant Number(s)
        2015-DN-BX-K020


  
  
        
  Agencies
        NIJ-Sponsored


  
  
        
  Publication Type
        Research (Applied/Empirical)
                                Report (Study/Research)
                                Report (Grant Sponsored)
                                Program/Project Description


  
  
        
  Award
        Proteomic and microRNA markers of fly development: Stable correlates of insect age to improve forensic entomology casework
    
  
  
Date Published: April 1, 2019
    
          Downloads
        
                      PDF

Side navigation
  

        

  
  
              NIJ Journal
    
      
            
            
            
      Podcasts
    
      
            
            
            
      Publications Listing
    
      
            
            
            
      Multimedia Listing
    
      
            
            
            
      Rated Practices on CrimeSolutions
    
      
            
            
            
      Rated Programs on CrimeSolutions
    
      
            
            
            
      Software & Databases
    
      
            
            
            
      Datasets
    
      
            
            
            
      PatentsRelated Topics
        
                Crime Scene
        Death investigations
        Decomposition
        Forensic entomology
        Forensic laboratories



  
Similar Publications
        
                      A New Tool for Probabilistic Assessment of MPS Data Associated with mtDNA Mixtures
                  Effect of Muzzle Gases on Forward Spatter of Viscoelastic Blood in Close-range Shooting
                  ICPUTRD: Image Cloud Platform for Use in Tagging and Research on Decomposition
 Top

      National Institute of Justice

        
        
                        About NIJ
                    About NIJ
      
    
                  How NIJ Is Organized
      
    
                  NIJ Director
      
    
                  Research Disclaimer
      
    
                  Funding & Awards
                    Funding & Awards Home
      
    
                  Awards Listing
      
    
                  Current Funding
      
    
                  Guidance for Applicants and Awardees
      
    
                  Resources
                    Events
      
    
                  Podcasts
      
    
                  Publications
      
    
                  Topics
      
    
                  Multimedia
      
    
                  Reuse Policy
      
    
                  Contacts
                    Contact NIJ
      
    
                  Staff Directory
      
    
                  Submit a FOIA Request
      
    
                  File a Civil Rights ComplaintFacebook
          
                      X
          
                      YouTube
  

      810 7th St. NW, Washington, D.C. 20531
      
              Secondary Footer link menu
            Accessibility
              Plain Language
              Privacy Policy
              Legal Policies and Disclaimer
              No FEAR Act
              Freedom of Information Act
              USA.gov
              Justice.gov