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                            Library & Multimedia
 A plastic  disposable microfluidic flow cell for coupled on-chip PCR and microarray detection of infectious agents
NCJ Number
              255503
Journal

*Biomedical Microdevices* Volume: 14 Dated: 2012 Pages: 45-53
Author(s)
          Christopher G. Cooney;
                        David Sipes;
                        Nitu Thakore;
                        Rebecca Holmberg;
                        Phillip Belgrader
Date Published

2012
Length
              9 pages
Annotation
              Since clinical laboratories are recognizing the importance of implementing sensitive and specific molecular diagnostic tests that require simplified workflows without requiring expensive supporting instrumentation, this article describes a valveless flow cell for disposable use that supports PCR coupled with microarray hybridization in the same chamber.
Abstract
              The flow cell assembly consists simply of double-faced tape, a plastic microarray substrate, an absorbent, and a commercially available hydrophilic thin film. The simple construction lends itself to low-cost and ease of manufacturing, and several features reduce the complexity of the standard microarray workflow. First, there is no requirement for custom instrumentation. Second, the hydrophilic thin film enables uniform filling of a microfluidic chamber. Third, a geometric capillary stop design confines liquid to the microarray chamber during PCR, and thus eliminates the need for a valve or hydrophobic surface treatment. And fourth, imbibition drives the uniform removal of liquid reagents from the array chamber. Three hundred genomic copies of methicillin-resistant Staphylococcus aureus (MRSA) are detected in a flow cell with gel drop microarrays printed on an unmodified plastic substrate. This sensitivity is shown to be comparable to conventional methods (i.e., PCR in a tube, with separate hybridization in a microarray chamber, where amplicon is exposed to the workspace before and after hybridization); however, the flow cell combines these multiple steps into a simple, compact workflow without the need for complex valves or custom instrumentation and is less susceptible to contamination of the workspace than conventional methods because the amplicon is confined to the device. (publisher abstract modified)
      
  
          Additional Details
Grant Number(s)
        2009-DN-BX-K189
                                2010-DN-BX-K209
                                2007-DN-BX-K145


  
  
        
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  Award
        A Microfluidic Microarray Instrument to Type SNPs for Physical Appearance
                                A Low Cost Microfluidic Microarray Instrument for Typing Y-Chromosome SNPS
                                A Low Cost Microfluidic Instrument for Typing SNPs
    
  
  
Date Published: January 1, 2012
    
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        DNA (Deoxyribonucleic Acid)
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