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                            Library & Multimedia
 Rapid DNA analysis for automated processing and interpretation of low DNA content samples
NCJ Number
              255529
Journal

*Investigative Genetics* Volume: 7 Issue: 2 Dated: 2016
Author(s)
          R. S. Turingan
Date Published

2016
Length
              0 pages
Annotation
              This article reports on a rapid DNA analysis system designed specifically to generate STR profiles from low DNA content (LDC) samples in field-forward settings by non-technical operators.
Abstract
              By performing STR in the field close to the site of collection, rapid DNA analysis has the potential to increase throughput and to provide actionable information in real time. Short tandem repeat (STR) analysis of casework samples with low DNA content include those resulting from the transfer of epithelial cells from the skin to an object (e.g., cells on a water bottle, or brim of a cap), blood spatter stains, and small bone and tissue fragments. Low DNA content (LDC) samples are important in a wide range of settings, including disaster response teams to assist in victim identification and family reunification, military operations to identify friend or foe, criminal forensics to identify suspects and exonerate the innocent, and medical examiner and coroner offices to identify missing persons. Processing LDC samples requires experienced laboratory personnel, isolated workstations, sophisticated equipment, transport time, and complex procedures. In the current project, A Low DNA Content BioChipSet (LDC BCS) was developed and manufactured by injection molding. It was designed to function in the fully integrated Accelerated Nuclear DNA Equipment (ANDE) instrument previously designed for analysis of buccal swab and other high DNA content samples (Investigative Genet. 4(1):1–15, 2013). The LDC BCS performs efficient DNA purification followed by microfluidic ultrafiltration of the purified DNA, maximizing the quantity of DNA available for subsequent amplification and electrophoretic separation and detection of amplified fragments. The system demonstrates accuracy, precision, resolution, signal strength, and peak height ratios appropriate for casework analysis. The project team concluded that The LDC rapid DNA analysis system is effective for the generation of STR profiles from a wide range of sample types. The technology broadens the range of sample types that can be processed and minimizes the time between sample collection, sample processing and analysis, and generation of actionable intelligence. The fully integrated Expert System can interpret a wide range of sample types and input DNA quantities, enabling samples to be processed and interpreted without a technical operator. (publisher abstract modified)
      
  
          Additional Details
Grant Number(s)
        2009-DN-BX-K256


  
  
        
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        NIJ-Sponsored


  
  
        
  Award
        DNA Analysis of LCN Samples: Towards Fully Integrated STR Profiling
    
  
  
Date Published: January 1, 2016
    
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                DNA (Deoxyribonucleic Acid)
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