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                            Library & Multimedia
 One of the Key Characteristics of Ancient DNA  Low Copy Number  May be a Product of its Extraction
NCJ Number
              255408
Journal

*Journal of Archaeological Science* Volume: 46 Pages: 281-289
Author(s)
          J. L. Barta;
                        C. Monroe;
                        J. E. Teisberg;
                        M. Winters;
                        M. Winters;
                        et al
Date Published

February 2022
Length
              9 pages
Annotation
              Since DNA from ancient sources is generally believed to be of low copy number, despite minimal attention towards direct measurement of DNA loss accumulated through the extraction process, the current study developed synthesized “standards” to measure the efficiency of some common DNA extraction methods for degraded skeletal samples, and used quantitative PCR to estimate a known quantity of DNA subjected to a given extraction method (i.e. “copies in”) versus quantity of DNA retained (i.e. “copies out”).
Abstract
              The study found that all methods performed poorly in retaining short segments of DNA, giving low copy number results even when pre-extraction copy numbers exceeded those expected of ancient samples. These findings challenge low copy number expectations, suggesting that ancient specimens may contain far more preserved genetic material than previously recognized. Furthermore, they emphasize the importance of optimizing and/or developing specialized extraction methods for retrieving degraded DNA. (publisher abstract modified)
      
  
          Additional Details
Grant Number(s)
        2008-DN-BX-K008


  
  
        
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        NIJ Proposal to Enhance Methods for Studying Degraded DNA
    
  
  
Date Published: February 18, 2022
    
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