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                            Library & Multimedia
 Optimization of InnoXtract (TM) extraction and purification system for DNA extraction from skeletal samples
NCJ Number
              307463
Journal

*International Journal of Legal Medicine* Dated: 2023 Pages: 949-959
Author(s)
          Jennifer Snedeker;
                        Sheree Hughes;
                        Rachel Houston
Date Published

2023
Length
              11 pages
Annotation
              This article describes the InnoXtractTM extraction and purification system, a purification method designed for DNA extraction from low-template samples, specifically rootless hair shafts, which successfully purified sufficient amounts of quality DNA from a variety of skeletal samples to produce complete STR profiles.
Abstract
              The InnoXtractTM extraction and purification system, a purification method designed for DNA extraction from low-template samples, specifically rootless hair shafts, successfully purified sufficient amounts of quality DNA from a variety of skeletal samples to produce complete STR profiles. Successful STR typing from surface decomposition, burned, cremated, buried, and embalmed remains indicates the potential of this new method for challenging human identification and missing-person cases. Its ability to successfully capture highly fragmented DNA suggests its suitability for use with other challenging sample types, including skeletal remains. However, the lysis and digestion parameters required modifications to successfully optimize the method for this sample type. A two-part digestion was developed utilizing a homebrew digestion buffer (0.5 M EDTA, 0.05% Tween 20, and 100 mM NaCl) and a supplemental lysis with the Hair Digestion Buffer included in the InnoXtractTM kit. Additionally, the magnetic bead volume was modified to improve DNA recovery from these challenging samples. With the altered protocol, the quality and quantity of DNA recovered from InnoXtractTM extracts were comparable to another commercial skeletal extraction method (PrepFilerTM BTA). (Published Abstract Provided)
      
  
          Additional Details
Grant Number(s)
        DJO-NIJ-21-RO-0002
                                2014-DNR-4918


  
  
        
  Agencies
        NIJ-Sponsored


  
  
        
  Publication Type
        Research (Applied/Empirical)


  
  
        
  Award
        Interagency Collaboration to Support Forensic Science Research
                                NIJ Co-funding for the NSF Center for Advanced Research in Forensic Science (CARFS)
    
  
  
Date Published: January 1, 2023
    
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