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                            Library & Multimedia
 Performance Evaluation and Optimization of Multiplex PCRs for the Highly Discriminating OSU 10-Locus Set Y-STRs
NCJ Number
              239615
Journal

*Journal of Forensic Sciences* Volume: 57 Issue: 1 Dated: January 2012 Pages: 52-59
Author(s)
          Erin Hanson, Ph.D.;
                        Julie L. Maybruck, Ph.D.;
                        Jack Ballantyne, Ph.D.;
                        Paul A. Fuerst, Ph.D.
Date Published

January 2012
Length
              8 pages
Annotation
              This study evaluated the OSU 10-locus set to test the effectiveness of the markers on forensic samples.
Abstract
              In a previous study, a new set of Y-chromosome short tandem repeats, the OSU 10-locus set (MPM1 and MPM2), was shown to have a higher discrimination power when evaluated against the 10 SWGDAM loci on a common population panel. Here, we describe the optimization of the multiplex reactions using dye-labeled primers followed by performance evaluations. The loci exhibited high precision, human male specificity, reliability in different body fluids, high sensitivity, stability, and the ability to amplify nonprobative casework and mixture samples. Stutter for the all of the loci, with the exception of the highly polymorphic locus DYS688, was similar to that observed for autosomal loci. The results of the performance evaluations reinforced the utility of these loci. Published by arrangement with John Wiley & Sons.
      
  
          Additional Details
Grant Number(s)
        2005-MU-MU-K044


  
  
        
  Agencies
        NIJ-Sponsored


  
  
        
  Publication Type
        Report (Study/Research)


  
  
        
  Award
        National Center for Forensic Science
    
  
  
Date Published: January 1, 2012
    
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