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                            Library & Multimedia
 Pre-Extraction Separation of Mixed Source DNA Samples Through Micromanipulation and Direct PCR Short Tandem Repeat Typing of Cells
NCJ Number
              251802

Author(s)
          Elisa  Wurmbach
Date Published

April 2016
Length
              79 pages
Annotation
              This study addressed the challenge of interpreting admixed STR profiles from multiple donors, particularly when dealing with initially small or trace amounts of cellular items, such as touched objects.
Abstract
              This was done by finding a method to collect single cells and the cellular material of fingerprints, by using instruments appropriate for micromanipulation, by separating samples and processing them independently, and by the examination of protocols to extract DNA and amplify STR loci to generate database-eligible profiles. In a discussion of future research on this issue, the study notes that although this study analyzed an enormous number of fingerprints, other laboratories could confirm the outcomes generated by the current study. This would assist in developing a more standardized protocol for handling fingerprints in forensic casework. Validating the Axiozoom for forensic casework could also be beneficial, since this instrument can be used on a variety of criminal cases in which mixed samples of two or more contributors are identified. 19 figures, 2 tables, and 61 references
      
  
          Additional Details
Grant Number(s)
        2012-DN-BX-K043


  
  
        
  Agencies
        NIJ-Sponsored


  
  
        
  Publication Type
        Research (Applied/Empirical)
                                Report (Study/Research)
                                Report (Grant Sponsored)
                                Program/Project Description


  
  
        
  Award
        Pre-Extraction Separation of Mixed Source DNA Samples through Micromanipulation and Direct PCR Short Tandem Repeat Typing of Cells
    
  
  
Date Published: April 1, 2016
    
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                DNA (Deoxyribonucleic Acid)
        DNA extraction
        DNA fingerprinting
        Forensic sciences
        Investigative techniques



  
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