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                            Library & Multimedia
 Optimization of Total Vaporization Solid-Phase Microextraction (TV-SPME) for the Determination of lipid profiles of Phormia Regina, a Forensically Important Blow Fly Species
NCJ Number
              253294

Author(s)
          William Kranz;
                        Clinton Carroll;
                        Darren Dixon;
                        Christine Picard;
                        John Goodpaster
Date Published

2017
Length
              9 pages
Annotation
              This article describes a new method that has been developed for the determination of fatty acids, sterols, and other lipids that naturally occur within pupae of the blow fly Phormia regina.
Abstract
              The method relies upon liquid extraction in non-polar solvent, followed by derivatization using N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) w/ 1 percent trimethylchlorsilane (TMCS) carried out inside the sample vial. The analysis is facilitated by total vaporization solid-phase microextraction (TV-SPME), with gas chromatography-mass spectrometry (GC-MS) serving as the instrumentation for analysis. The TV-SPME delivery technique is approximately a factor of five more sensitive than traditional liquid injection, which may alleviate the need for rotary evaporation, reconstitution, collection of high performance liquid chromatography fractions, and many of the other pre-concentration steps that are commonplace in the current literature. Furthermore, the ability to derivatize the liquid extract in a single easy step while increasing sensitivity represents an improvement over current derivatization methods. The most common lipids identified in fly pupae were various saturated and unsaturated fatty acids ranging from lauric acid (12:0) to arachinoic acid (20:4), as well as cholesterol. The concentrations of myristic acid (14:0), palmitelaidic acid (16:2), and palmitoleic acid (16:1) were the most reliable indicators of the age of the pupae. (publisher abstract modified)
      
  
          Additional Details
Grant Number(s)
        2013-DN-BX-K019


  
  
        
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        NIJ-Sponsored


  
  
        
  Publication Type
        Research (Applied/Empirical)
                                Report (Study/Research)
                                Report (Grant Sponsored)
                                Program/Project Description


  
  
        
  Award
        Species and age determination of blow fly pupae based upon headspace analysis
    
  
  
Date Published: January 1, 2017
    
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