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                            Library & Multimedia
 Quantification of hordenine in a complex plant matrix by direct analysis in real time-high-resolution mass spectrometry: Application to the "plant of concern" Sceletium tortuosum
NCJ Number
              307150
Journal

*Drug Testing and Analysis* Volume: 14 Issue: 4 Dated: 2022 Pages: 604-612
Author(s)
          Meghan Grace Appley;
                        Megan Isabella Chambers;
                        Rabi Ann Musah
Date Published

2022
Length
              9 pages
Annotation
              This paper discusses the possibility of using direct analysis in real time of high-resolution mass spectrometry to identify Sceletium Tortuosum, and products derived from it, as well as to quantify the amount of hordenine present.
Abstract
              Recently, there has been an increase in the recreational abuse of several psychoactive plants, resulting in the United Nations Office on Drugs and Crime creating a list of “plants of concern.” One such material is Sceletium tortuosum and products derived from it. Regulation of these materials is challenging because of their innocuous appearance, the cumbersome sample preparation steps required to render the material into a form amenable to analysis by conventional techniques, the requirement for nuanced sample analysis protocols, and lengthy analysis times. It is demonstrated here that direct analysis in real-time; high-resolution mass spectrometry (DART-HRMS) can be used to not only identify S. tortuosum material based on the detection of characteristic biomarkers including hordenine and several mesembrine alkaloids, but it can also be used to quantify the amount of hordenine present. Using hordenine-d6 as an internal standard, a protocol, validated according to US Food and Drug Administration (FDA) Guidelines for the Development and Validation of Bioanalytical Methods, was devised for the quantification of the psychoactive component hordenine. The method was then applied to the quantification of hordenine in six commercially available products derived from the foliage and stems of S. tortuosum. By this method, the lower limit of quantification (LLOQ) was found to be one μg/ml. The developed technique provides an effective and quick means for the detection and quantification of hordenine in S. tortuosum, which can be extended to analysis of other hordenine-containing products. Publisher Abstract Provided
      
  
          Additional Details
Grant Number(s)
        2018-R2-CX-0012
                                2019-DU-BX-0026


  
  
        
  Agencies
        NIJ-Sponsored


  
  
        
  Publication Type
        Research (Applied/Empirical)


  
  
        
  Award
        Detection and Identification of Psychoactive Material Based On SPME-facilitated DART-MS-derived Headspace Chemical Signatures and Chemometrics
                                Research to Develop Validated Methods for THC Quantification in Complex Matrices by High-resolution DART-MSFocus on Edibles and Plant Materials
    
  
  
Date Published: January 1, 2022
    
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