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                            Library & Multimedia
 New Methods for Next-Generation Sequencing Analysis of Damaged and Mixed DNA Samples
NCJ Number
              254589

Date Published

April 2020
Length
              2 pages
Annotation
              This article details a technique to make fragmented DNA more readable and a method to help pull individual profiles from mixed samples.
Abstract
              Investigators who use next generation DNA sequences to identify individuals may encounter degraded or mixed samples. Using funding from the National Institute of Justice (NIJ), Cassandra Calloway, a scientist at Children's Hospital Oakland Research Institute (California), has developed a technique to make fragmented DNA more discernible. She also designed, tested, and implemented an analytic tool that generates individual DNA profiles from a variety of mixture proportions. Both of these methods can be used for DNA profiling of samples too aged or damaged, such as those from the 9/11 attacks or the recent California wildfires, to produce any identifying information using standard forensic short tandem repeats. This article describes Calloway's research.
      
  
          Additional Details
Grant Number(s)
        2013-MU-MU-K044


  
  
        
  Agencies
        NIJ


  
  
        
  Publication Type
        Report (Technical Assistance)
                                Report (Study/Research)
                                Program/Project Description
                                Grants and Funding
                                Factsheet


  
  
        
  Award
        Development of Probe Capture Next-Generation Sequencing Assays for Degraded DNA
    
  
  
Date Published: April 1, 2020
    
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      PatentsRelated Topics
        
                Degraded DNA samples
        DNA (Deoxyribonucleic Acid)
        DNA typing
        Forensic laboratories
        Forensic sciences



  
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