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                            Library & Multimedia
 Modified DOP-PCR for Improved STR Typing of Degraded DNA From Human Skeletal Remains and Bloodstains
NCJ Number
              250028

Author(s)
          A. Ambers, M. Turnbough
Date Published

January 2016
Length
              6 pages
Annotation
              Several strategies have been proposed to address the challenges posed by degraded and low copy templates, including a PCR-based whole genome amplification method called degenerate oligonucleotide-primed PCR (DOP-PCR). This study assessed the efficacy of four modified versions of the original DOP-PCR primer that retain at least a portion of the 5Œ defined sequence and alter the number of bases on the 3Œ end.
Abstract
              The use of each of the four modified primers resulted in improved STR profiles from environmentally-damaged bloodstains, contemporary human skeletal remains, American Civil War era bone samples, and skeletal remains of WWII soldiers over those obtained by previously described DOP-PCR methods and routine STR typing. Additionally, the modified DOP-PCR procedure allows for a larger volume of DNA extract to be used, reducing the need to concentrate the sample and thus mitigating the effects of concurrent concentration of inhibitors.  (Publisher abstract modified)
      
  
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  Award
        Addressing Quality and Quantity; the Role of DNA Repair and Whole Genome Amplification in Forensically Relevant Samples
    
  
  
Date Published: January 1, 2016
    
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