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                            Library & Multimedia
 Methods for Obtaining STR-Quality Touch DNA from Archived Fingerprints
NCJ Number
              252769

Author(s)
          Tracey  Dawson Cruz;
                        Susan E. Robb
Date Published

March 2017
Length
              23 pages
Annotation
              This is the final summary report on a project that evaluated analytical procedures for the processing of archived, paper-backed, latent fingerprint lifts, so as to make recommendations to crime scene, criminal justice, and forensic DNA practitioners on processing archived prints in the laboratory.
Abstract
              The project provides evidence that should encourage forensic laboratories and investigators to consider doing DNA analysis from archived latent fingerprints, particularly when they are the only potential source of physical or biological evidence available. Viable DNA was found to be available in some archived latent fingerprint samples, as well as in the surface area left behind after swabbing, and it can be retrieved for DNA profiling. The findings indicate that when processing archived latent fingerprints for DNA analysis, disassembly of the fingerprint "sandwich" and making direct cuttings of the latent print area results in more template DNA available for downstream amplification and improved STR profiles compared with commonly used swabbing methods; however, the research also indicates that the double-swabbing method can be a reliable method and may perform best when the initial swab is pre-wet with a diluent that includes a lysis buffer and protein-degrading enzyme. In addition, the use of a silica-based column extraction method that has been optimized for use with forensic/challenged samples, followed by a gel-column concentration method, may increase the chances of obtaining informative STR profiles when used instead of other common methods/combinations. On the other hand, the research indicated that the addition of a post-amplification purification step did not improve the STR profiles obtained from these samples. The increased sensitivity is more likely to increase the presence of artifacts that complicate data interpretation. Materials and methods are described. 50 references
      
  
          Additional Details
Grant Number(s)
        2014-DN-BX-K013


  
  
        
  Agencies
        NIJ-Sponsored


  
  
        
  Publication Type
        Research (Applied/Empirical)
                                Report (Study/Research)
                                Report (Grant Sponsored)
                                Program/Project Description


  
  
        
  Award
        Methods for obtaining STR???quality touch DNA from archived fingerprints
    
  
  
Date Published: March 1, 2017
    
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