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                            Library & Multimedia
 microRNA Detection in Blood, Urine, Semen, and Saliva Stains After Compromising Treatments
NCJ Number
              302631
Journal

*Journal of Forensic Sciences* Volume: 64 Issue: 6 Dated: 2019 Pages: 1831-1837
Author(s)
          T. R Layne;
                        et al
Date Published

2019
Length
              7 pages
Annotation
              This article presents the findings and methodology of a study that involved the use of microRNA to detect blood, urine, semen, and saliva stains after compromising treatments.
Abstract
              Evaluation of microRNA (miRNA) expression as a potential method for forensic body fluid identification has been the subject of investigation over the past several years. Because of their size and encapsulation within proteins and lipids, miRNAs are inherently less susceptible to degradation than other RNAs. In this work, blood, urine, semen, and saliva were exposed to environmental and chemical conditions mimicking sample compromise at the crime scene. For many treated samples, including 100% of blood samples, miRNAs remained detectable, comparable to the untreated control. Sample degradation varied by body fluid and treatment, with blood remarkably resistant, while semen and saliva are more susceptible to environmental insult. Body fluid identification using relative miRNA expression of blood and semen of the exposed samples was 100 percent and 94 percent, respectively. Given the overall robust results herein, the case is strengthened for the use of miRNAs as a molecular method for body fluid identification. (publisher abstract modified)
      
  
          Additional Details
Grant Number(s)
        2012-DN-BX-K017
                                2016-DN-BX-0163


  
  
        
  Agencies
        NIJ-Sponsored


  
  
        
  Award
        Deep Sequencing for Identification and Characterization of MicroRNAs in Forensically Important Biological Fluids
                                Developmental validation of a miRNA panel for forensic body fluid identification
    
  
  
Date Published: January 1, 2019
    
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