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                            Library & Multimedia
 Massively Parallel Sequencing of the Mitogenome from Human Hair Shafts in Forensic Investigations
NCJ Number
              307438

Author(s)
          Jade T. Korber;
                        Lauren C. Canale;
                        Mitchell M. Holland
Date Published

2023
Annotation
              This article discusses protocols for performing DNA extraction, mitochondrial DNA quantification, multiplex PCR amplification, amplicon-based massively parallel sequencing, and data analysis of the mitochondrial genome (mitogenome) from human hair shafts.
Abstract
              This article highlights methods used to perform DNA extraction, mitochondrial DNA quantification, multiplex PCR amplification, amplicon-based massively parallel sequencing, and data analysis of the mitochondrial genome (mitogenome) from human hair shafts. The focus is on applications to forensic casework, but this set of protocols can be used for any purpose involving small cuttings (as small as 1 to 5 mm) of human hair shafts up to 40 years from the time of collection. The protocols include: Basic Protocol 1: Extraction of mitochondrial DNA from human hair shafts; Basic Protocol 2: Quantification of mitochondrial DNA (copies/μl); Basic Protocol 3: Multiplex amplification of the mitogenome; Basic Protocol 4: Library preparation and sequencing of mitogenome amplicons; Basic Protocol 5: Data analysis of mitogenome haplotypes. (Published Abstract Provided)
      
  
          Additional Details
Grant Number(s)
        2019-DU-BX-0045


  
  
        
  Agencies
        NIJ-Sponsored


  
  
        
  Publication Type
        Research (Applied/Empirical)


  
  
        
  Award
        mtGenome Sequencing of Human Hair Shafts Using an MPS Approach
    
  
  
Date Published: January 1, 2023
    
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        Forensic biology
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        Research and Development in Forensic Science for Criminal Justice Purposes
        DNA (Deoxyribonucleic Acid)



  
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