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Title:   Author  item
                                            CORREA, ALEJANDRO - Universidad Autonoma De Chihuahuaitem
                                            TRACHSEL, JULIAN - Iowa State Universityitem
                                            Allen, Heatheritem
                                            CORRAL-LUNA, AGUSTIN - Universidad Autonoma De Chihuahuaitem
                                            Hume, Michaelitem
                                            Callaway, Todditem
                                            Anderson, Robinitem
                                            Nisbet, David
  Submitted to: Meeting Proceedings
                                    Publication Type: Proceedings
                                    Publication Acceptance Date: 9/27/2015
                                    Publication  Date: 10/28/2016
                                    Citation: Correa, A.C., Trachsel, J., Allen, H.K., Corral-Luna, A., Hume, M.E., Callaway, T.R., Anderson, R.C., Nisbet, D.J. 2016. Effect of sole or combined administration of methane inhibitors nitrate and 3-nitro-1-propionate on rumen in vitro fermentation. Meeting Proceedings. 1(2015):104-415.Interpretive Summary:
                                Technical Abstract: Nitrate and 3-nitro-1-propionate are nitro-compounds capable of inhibiting ruminal methanogenesis.  In order to assess sub-toxic concentrations of sole and combined nitrate and 3-nitro-1-propionate on methane production and rumen fermentation, an experiment was conducted using 4 μmol of 3-nitro-1-propionate, 8 μmol of nitrate, 16 μmol of nitrate or combinations of 4 μmol of 3-nitro-1-propionate with either 8 to 16 μmol of nitrate per mL of incubation fluid under a randomized block design.  All treatments reduced (P < 0.05) methane production (> 96%) compared to the control.  The hydrogen concentration was less than 50% compared to control.  All of the sole 3-nitro-1-propionate added was metabolized.  The 3-nitro-1-propionate and nitrate were catabolized in less than 17% and greater than 95%, respectively.  However, the concentration of nitrite in the treatments with added nitrate ranged from 3 to 12 μmol/mL, representing between 38% and 69% of the nitrate added, respectively.  Total concentration of volatile fatty acids decreased (P < 0.05) greater than 32% with sole treatment and greater than 46% for the combined treatments compared to the control.  The acetate:propionate ratio increased (P < 0.05) 1.3- to 3-fold in all treatments.  The hydrogen balance indicated that fermentative production of reductant was decreased in all treatments, although the effect was pronounced when added in combination.  The addition of 3-nitro-1-propionate and nitrate decreased methane production, but also decreased rumen fermentation.  These results will serve as a foundation for continued investigations regarding the inhibitory effects of nitrocompounds on rumen methanogenesis.


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