U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Infectious Bacterial Diseases Research: Ames, IA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area Ames, Iowa National Animal Disease Center Infectious Bacterial Diseases Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Waters, Wadeitem
                                            NELSON, JEFF - Animal And Plant Health Inspection Service (APHIS)item
                                            Thacker, Tyleritem
                                            MAGGIOLI, MAYARA - Iowa State Universityitem
                                            STAFNE, MOLLY - Iowa State Universityitem
                                            BASS, KRISTIN - Bethyl Laboratories, Incitem
                                            LINSCOTT, RICK - Idexx Laboratoriesitem
                                            LAWRENCE, JOHN - Idexx Laboratoriesitem
                                            Palmer, Mitchell
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 11/6/2014
                                    Publication  Date: 11/6/2014
                                    Citation: Waters, W.R., Nelson, J., Thacker, T.C., Maggioli, M., Stafne, M., Bass, K., Linscott, R., Lawrence, J., Palmer, M.V. 2014. Effect of skin test on serum antibody responses to Mycobacterium bovis infection in cattle [abstract]. United States Animal Health Association. Abstract No. 22.Interpretive Summary:
                                Technical Abstract: Recently, several serologic tests designed to detect immunodominant antibodies to M. bovis antigens (e.g., MPB83, MPB70, ESAT-6, and CFP10) have emerged for potential use with samples from cattle. Of these, a commercial ELISA to MPB83/MPB70 (M. bovis antibody ELISA) has gained approval for use in cattle for bovine tuberculosis control programs by the Office International des Epizooties and United States Department of Agriculture. In the present study, the effect of injection of purified protein derivatives (PPD) for caudal fold (CFT) and comparative cervical (CCT) skin tests on serum antibody responses were evaluated with samples from cattle experimentally-infected with M. bovis (n = 8, aerosol challenge). Injection of M. bovis PPD for CFT (89 days after aerosol challenge) elicited serum antibody responses detectable within 1 week by the IDEXX M. bovis antibody ELISA. Positive responses were detectable in all animals up to 74 days after PPD administration. Injection of M. avium and M. bovis PPDs for CCT (105 days after CFT) resulted in a dramatic increase in antibody responses in all animals. Antibody avidity, as measured by an ammonium thiocyanate assay, also increased upon injection of PPDs for CCT. These findings demonstrate that the anamnestic response elicited by injection of PPD(s) for skin test results in both qualitative and quantitative increases in serum antibody responses in M. bovis-infected cattle, of diagnostic relevance.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov