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Title:   Author  item
                                            MALMIERCA, M - University Of Leonitem
                                            BARUA, J - University Of Cadizitem
                                            McCormick, Susanitem
                                            CARDOZA, R - University Of Leonitem
                                            ALEXANDER, N - Retired ARS Employeeitem
                                            IZQUIERDO-BUENO, I - University Of Cadizitem
                                            BARREIRO, C - Inbiotecitem
                                            COLLADO, I - University Of Cadizitem
                                            MONTE, E - University Of Salamanca
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 6/11/2015
                                    Publication  Date: 6/11/2015
                                    Citation: Malmierca, M.G., Barua, J., McCormick, S.P., Cardoza, R.E., Alexander, N.J., Izquierdo-Bueno, I., Barreiro, C., Collado, I.G., Monte, E. 2015. Regulation of Botrytis cinerea virulence genes in interaction with Trichoderma arundinaceum is mediated by the sesquiterpene harzianum A. Meeting Abstract. 0710.Interpretive Summary:
                                Technical Abstract: Trichoderma includes a great diversity of species, some of them with the ability to control the growth of fungal phytopathogens. Many of these strains produce secondary metabolites that are able to inhibit the growth of their fungal preys. However, pathogens can also produce metabolites that in some cases belong to the same family of those produced by Trichoderma. In the present work we study the interaction between Harzianum A (HA) produced by T. arundinaceum (Ta37) and botrydial (BOT) produced by Botrytis cinerea. HA exhibits antifungal activity, and BOT is a strong virulence factor in plant disease. A procedure of confrontation between these two strains was implemented, followed by the analysis of gene expression by real-time PCR using mycelia isolated from the confrontation areas. To better characterize the regulatory responses produced by HA, the TaDTri5 mutant, isolated by disruption of tri5 gene and blocked in the synthesis of HA, was also used. It was observed that HA induced expression of genes involved in BOT biosynthesis when HA was added to B. cinerea growing alone. However, in confrontation experiments against TaDTri5 the expression of these genes was higher than in confrontation with Ta37, indicating that the mutant must produce some metabolite/s, responsible for such induction. A different metabolite production pattern was described for Ta37 and TaDTri5, which is in agreement with the proteomic characterization of these two strains, suggesting that genetic disruption of tri5 had a systemic effect of the fungal physiology not only restricted to the terpene pathway.


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