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Title:   Author  item
                                            GRANSTROM  DAVID  - GLUCK EQUINE RES CTR, KYitem
                                            MACPHERSON  J M  - HLTH ANML LAB, CANADAitem
                                            GAJADHAR  ALVIN A  - HLTH ANML LAB, CANADAitem
                                            Dubey, Jitenderitem
                                            TRAMONTIN  ROBERT  - GLUCK EQUINE RES CTR, KYitem
                                            STAMPER SHELBY  - GLUCK EQUINE RES CTR, KY
  Submitted to: Molecular and Cellular Probes
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 6/7/1994
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary: Equine protozoal myeloencephelits (EPM) is a fatal disease of horses.  The life cycle and source of infection for EPM are unknown.  EPM affects the young and old horses and the disease is confined to the Americas.  Most cases of EPM are diagnosed after death. The etiologic agent of EPM, Sarcocystis neurona, was recently grown in cell culture.  Scientists at  the Agricultural Research Center and the University of Kentucky have isolated a fragment (portion) of DNA from culture derived Sarcocystis neurona.  The results will be useful for the development of a DNA based test for the diagnosis of EPM in live horses.
                                Technical Abstract: Four isolates of Sarcocystis neurona, obtained from horses with equine protozoal myeloencephalitis and eight species of coccidia from the genera Sarcocystis, Toxoplasma, and Eimeria were differentiated using the random amplified polymorphic DNA assay.  A single, common 550 bp DNA fragment was amplified from the DNA of each S. neurona isolate using a 16-nucleotide  primer.  Cross hybridization analyses among S. neurona isolates showed that DNA fragments had at least partial sequence homology.  The primer generated several DNA fragments, including a 550 bp DNA fragments, from S. cruzi, Eimeria falciformis, E. neischulzi, E. ahsata and E. bovis.  DNA hybridization analyses indicated no sequence homology between these fragments and the 550 DNA fragment generated from S. neurona.  The S. neurona 550 bp DNA fragment also did not hybridize with genomic blots of various other coccidia. These results suggest that the S. neurona DNA  fragment may be exploited as a species-specific probe for this parasite.


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