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Title:   Author  item
                                            Madeira, Paulitem
                                            FACEY, JORDAN - St Thomas Universityitem
                                            Pratt, Paulitem
                                            MAUL, DORA PILAR - St Thomas Universityitem
                                            Wheeler, Gregory
  Submitted to: Biocontrol Science and Technology
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 11/25/2015
                                    Publication  Date: 1/11/2016
                                    Citation: Madeira, P.T., Facey, J., Pratt, P.D., Maul, D., Wheeler, G.S. 2016. Are three colonies of Neostromboceros albicomus, a candidate biological control agent for Lygodium microphyllum, the same host biotype? Biocontrol Science and Technology. 26(3):440-445.Interpretive Summary: Three colonies of the sawfly Neostromboceros albicomus, a candidate biological control agent of Lygodium microphyllum, were genetically fingerprinted to determine which of two biotypes they represented. The first colony was used for the initial host range testing in 2006. Colonies collected in 2012 and 2014 are currently being used to finalize this testing. All three colonies were collected in Trat Province, Thailand and all three populations show identical sequences to the N. albicomus biotype which prefers L. microphyllum as its host plant. The N. albicomus biotype that uses L. flexuosum as its host plant differs by a single nucleotide, a difference which may reflect cryptic speciation.
                                Technical Abstract: Three colonies of Neostromboceros albicomus, a candidate biological control agent of Lygodium microphyllum, were barcoded using the D2 expansion domain, to determine which of two biotypes they represented. The first colony, collected in 2005 & 2007, was used for the initial host range testing. Colonies collected in 2012 and 2014 are currently being used to finalize this testing. All three colonies were collected in Trat Province, Thailand and all three populations show identical sequences to the N. albicomus biotype which prefers L. microphyllum as its host plant. The N. albicomus biotype that uses L. flexuosum as its host plant differs by a single nucleotide, a difference which may reflect cryptic speciation.


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