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ARS Home Northeast Area Geneva, New York Grape Genetics Research Unit (GGRU) Research Publications at this Location





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Title:   Author  item
                                            YANG, SHANSHAN - Cornell Universityitem
                                            FRESNEDO, JONATHAN - Cornell Universityitem
                                            WANG, MINGHUI - Cornell Universityitem
                                            COTE, LINDA - Cornell Universityitem
                                            SCHWEITZER, PETER - Cornell Universityitem
                                            BARBA, PAOLA - Cornell Universityitem
                                            TAKACS, ELIZABETH - Cornell Universityitem
                                            CLARK, MATT - University Of Minnesotaitem
                                            LUBY, JIM - University Of Minnesotaitem
                                            MANNS, DAVID - Cornell Universityitem
                                            SACKS, GAVIN - Cornell Universityitem
                                            MANSFIELD, ANNA KATHERINE - Cornell Universityitem
                                            Londo, Jasonitem
                                            FENNELL, ANNE - South Dakota State Universityitem
                                            GADOURY, DAVID - Cornell Universityitem
                                            REISCH, BRUCE - Cornell Universityitem
                                            Cadle-Davidson, Lanceitem
                                            SUN, QI - Cornell University
  Submitted to: Plant and Animal Genome Conference
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 10/31/2015
                                    Publication  Date: 1/1/2016
                                    Publication URL: https://pag.confex.com/pag/xxiv/meetingapp.cgi/Paper/18715Citation: Yang, S., Fresnedo, J., Wang, M., Cote, L., Schweitzer, P., Barba, P., Takacs, E., Clark, M., Luby, J., Manns, D., Sacks, G., Mansfield, A., Londo, J.P., Fennell, A., Gadoury, D., Reisch, B., Cadle Davidson, L.E., Sun, Q. 2016. A Next-Generation Marker Genotyping Platform (AmpSeq) in Heterozygous Crops: A Case Study for Marker Assisted Selection in Grapevine. Plant and Animal Genome Conference.Interpretive Summary:
                                Technical Abstract: Marker assisted selection (MAS) has become widely used in perennial crop breeding programs to accelerate and enhance cultivar development via selection during the juvenile phase and parental selection prior to crossing. Next generation sequencing (NGS) has been widely used for whole genome molecular marker discovery, but it also offers a potential opportunity for tailored molecular marker development with high-throughput and low per-sample cost. This study presents a novel and efficient MAS strategy for molecular marker development incorporating a semi-automated primer design pipeline based on amplicon sequencing (AmpSeq). The results document the development of an MAS package using three relevant traits (flower sex, disease resistance, and modified anthocyanins) in grapevine breeding. Here we discuss several strengths of the amplicon sequencing platform that make this approach of broad interest in diverse crop species: accuracy, flexibility, high throughput, low cost, easily automated analysis, and rapid results.


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