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Title:   Author  item
                                            LI, XIN - University Of Minnesotaitem
                                            SHIN, SANGHYUN - University Of Minnesotaitem
                                            HEINEN, SHANE - University Of Minnesotaitem
                                            DILL-MACKY, RUTH - University Of Minnesotaitem
                                            BERTHILLER, FRANZ - University Of Natural Resources & Applied Life Sciences - Austriaitem
                                            CLEMENTE, THOMAS - University Of Nebraskaitem
                                            McCormick, Susanitem
                                            Chao, Shiaomanitem
                                            MUEHLBAUER, GARY - University Of Minnesota
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 12/9/2014
                                    Publication  Date: 12/9/2014
                                    Citation: Li, X., Shin, S., Heinen, S., Dill-Macky, R., Berthiller, F., Clemente, T., McCormick, S.P., Chao, S., Muehlbauer, G.J. 2014. Transgenic wheat and barley carrying a barley UDP-glucosyltransferase exhibit high levels of Fusarium head blight resistance [abstract].Interpretive Summary:
                                Technical Abstract: Fusarium head blight (FHB) is an old yet unsolved problem of cereal crops, mainly caused by the fungal pathogen Fusarium graminearum. During infection, trichothecenes produced by Fusarium increase fungal virulence and decrease grain quality. Previous work identified a barley UDP-glucosyltransferase gene (HvUGT13248) that detoxifies deoxynivalenol (DON) by the conversion to DON-3-O-glucoside (D3G) in transgenic yeast and Arabidopsis. Here we report successful development of transgenic wheat and barley overexpressing HvUGT13248 gene. The transgenic wheat show high levels of FHB type II resistance in the greenhouse point inoculation tests. The FHB severity of the transgenic lines were reduced by up to 91% compared to untransformed lines. We also performed spray inoculation tests on these transgenic wheat in the field across three consecutive years, and they also show high levels of FHB resistance. Moreover, transgenic wheat carrying HvUGT13248 converted DON to D3G more rapidly than untransformed plants, and there was also reduced DON accumulation in the grains of the transgenic wheat harvested from the field tests. We developed a fast and convenient method to screen wheat and barley resistance to trichothecenes, by monitoring root growth of seedlings on trichothecene-containing growth media. We used this root assay to show that transgenic barley overexpressing HvUGT13248 exhibit resistance to DON. We also introduced the HvUGT13248 transgene into the elite wheat cultivar Rollag, and the backcross-derived lines exhibited high levels of FHB resistance in the greenhouse and field tests, however, the FHB severity levels were not significantly different from Rollag. This result suggests that the FHB resistance mechanisms provided by the Fhb1 QTL and the HvUGT13248 transgene may overlap.


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