U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Vegetable Crops Research: Madison, WI
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    ScientistsSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area Madison, Wisconsin Vegetable Crops Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            SCHLAUTMAN, BRANDON - University Of Wisconsinitem
                                            COVARRUBIAS-PAZARAN, GIOVANNY - University Of Wisconsinitem
                                            FAJARDO, DIEGO - National Center For Genome Resourcesitem
                                            Steffan, Shawnitem
                                            Zalapa, Juan
  Submitted to: Genetic Resources and Crop Evolution
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 1/18/2016
                                    Publication  Date: 2/2/2016
                                    Citation: Schlautman, B., Covarrubias-Pazaran, G., Fajardo, D., Steffan, S., Zalapa, J. 2016. Discriminating power of microsatellites in cranberry organelles for taxonomic studies in Vaccinium and Ericaceae. Genetic Resources and Crop Evolution. 64(3):451-466. doi: 10.1007/s10722-016-0371-6.Interpretive Summary: This study mined, tested, and validated 24 mitochondrial and and 30 plastid microsatellite loci (i.e., molecular markers) from cranberry. The inherent variability of the markers and ability to transfer across multiple species, in combination with DNA genotyping highthrouhput approaches, suggests that they could be useful for quickly studying the molecular relationships for the Vaccinium genus and the entire Ericaceae family, which include several commercial important species such as cranberry, blueberry, lingonberry, etc. Increased understanding of genetic relationships in the Ericaceae family could shed light on the evolution of unique adaptations in the family (e.g., non-green vs. green, parasitic vs. mycotrophic, insectivorous vs. non-insectivorous, heavy metal tolerant vs. hyper-accumulators, different types of specialized and common mycorrhizal associations, etc.), and help implement species to species hybridization strategies for improving economically important Vaccinium berry crops such as blueberry and cranberry.
                                Technical Abstract: Simple sequence repeats (SSRs) in chloroplast and mitochondrial DNA, which have not been previously developed or explored in the Ericaceae family or Vaccinium genus, can be powerful tools for determining evolutionary relationships between taxa. In this study, 30 chloroplast and 23 mitochondria, and 1 mitochondria-like SSRs were identified in cranberry, and primer-pairs were developed and tested for each locus. Although no polymorphisms were detected for any of the 54 SSR loci in nine diverse cranberry (V. macrocarpon) genotypes, all primers were cross-transferable to some extent in a panel of 12 additional Vaccinium taxa and four non-Vaccinium Ericaceae species. A Neighbor-Joining tree of the estimated average square distances (D1) resolved the species by genera and by section within Vaccinium. Similar topologies with increased branch support were observed in Bayesian inference trees constructed using the DNA sequences of six plastid and two mitochondria SSR loci. Two multiplexing/poolplexing panels of M13 fluorescently labeled primers, which amplify 24 of the 54 markers, were developed and will serve as an efficient and cost-effective means for characterizing the basic molecular phylogeny currently lacking in Vaccinium. Increased understanding of the evolutionary relationships among Vaccinium species should facilitate interspecific hybridization and introgression efforts to improve economically important traits of commercial berry crops.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov