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Title:   Author  item
                                            GRANSTROM  DAVID  - GLUCK EQUINE RES CTR, KYitem
                                            MACPHERSON  J M  - HLTH ANML LAB, CANADAitem
                                            GAJADHAR  ALVIN A  - HLTH ANML LAB, CANADAitem
                                            Dubey, Jitenderitem
                                            TRAMONTIN  ROBERT  - GLUCK EQUINE RES CTR, KYitem
                                            STAMPER  SHELBY  - GLUCK EQUINE RES CTR, KY
  Submitted to: American Association of Veterinary Parasitologists Proceedings
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 7/11/1994
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: The random amplified polymorphic DNA assay was used to compare four isolates of Sarcocystis neurona and eight species of coccidia from the genera Sarcocystis, Toxoplasma, or Eimeria.  A single, common 550 bp DNA fragment was amplified from the DNA of each S. neurona isolate using a 16-nucleotide primer.  Cross hybridization analyses among S. neurona  isolates showed that DNA fragments had at least partial sequence homology.  The primer generated several DNA fragments, including a 550 bp DNA fragment, from the related coccidia.  DNA hybridization analyses indicated no sequence homology between these fragments and the 550 bp DNA fragment generated from S. neurona.  The S. neurona 550 bp DNA fragment also did not hybridize with genomic blots of various related coccidia. These results suggest that the S. neurona DNA fragment may be useful as a species-specific probe.


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