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Title:   Author  item
                                            TEH, SOON LI - University Of Minnesotaitem
                                            CLARK, MATT - University Of Minnesotaitem
                                            FRESNEDO, JONATHAN - Cornell Universityitem
                                            SUN, QI - Cornell Universityitem
                                            Cadle-Davidson, Lanceitem
                                            LUBY, JIM - University Of Minnesota
  Submitted to: Plant and Animal Genome Conference
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 10/31/2015
                                    Publication  Date: 1/1/2016
                                    Publication URL: https://pag.confex.com/pag/xxiv/meetingapp.cgi/Paper/19849Citation: Teh, S., Clark, M., Fresnedo, J., Sun, Q., Cadle Davidson, L.E., Luby, J. 2016. Genetic dissection of powdery mildew resistance in interspecific half-sib grapevine families using SNP-based maps. Plant and Animal Genome Conference. 1.Interpretive Summary:
                                Technical Abstract: Quantitative trait locus (QTL) identification in perennial fruit crops is impeded largely by their lengthy generation time, resulting in costly and labor-intensive maintenance of breeding programs. In a grapevine (genus Vitis) breeding program, although experimental families are typically unreplicated, the genetic backgrounds may contain similar progenitors previously selected due to their contribution of favorable alleles. In this study, we investigated the utility of joint QTL identification provided by analyzing half-sib families. The genetic control of powdery mildew was studied using two half-sib F1 families, namely GE0711/1009 (MN1264 × MN1214; N = 147) and GE1025 (MN1264 × MN1246; N = 125) with multiple species in their ancestry. Maternal genetic maps consisting of 1,077 and 1,641 single nucleotide polymorphism (SNP) markers respectively were constructed using a pseudo-testcross strategy. Ratings of field resistance to powdery mildew were obtained based on whole-plant evaluation of disease severity. This two-year analysis uncovered two QTLs that were validated on a consensus map in these half-sib families with improved precision relative to the parental maps. Examination of haplotype combinations based on the two QTL regions identified strong association of haplotypes inherited from Seyval blanc, through MN 1264, with powdery mildew resistance. This investigation also encompassed the use of SSR markers to establish a correlation between 206-bp (UDV-015b) and 357-bp (VViv67) fragment sizes with resistance-carrying haplotypes. Our work is one of the first reports in grapevine demonstrating the use of SNP-based maps and haplotypes for QTL identification and tagging of powdery mildew resistance in half-sib families.


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