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Title:   Author  item
                                            MUEHLBAUER, GARY - University Of Minnesotaitem
                                            LI, XIN - University Of Minnesotaitem
                                            SHIN, SANGHYUN - University Of Minnesotaitem
                                            DILL-MACKY, RUTH - University Of Minnesotaitem
                                            BERTHILLER, FRANZ - University Of Natural Resources & Applied Life Sciences - Austriaitem
                                            CLEMENTE, THOMAS - University Of Nebraskaitem
                                            McCormick, Susanitem
                                            KUMLEHN, JOCHEN - Leibniz Institute Of Plant Genetics And Crop Plant Researchitem
                                            ADAM, GERHARD - University Of Natural Resources & Applied Life Sciences - Austria
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 7/2/2014
                                    Publication  Date: 7/2/2014
                                    Citation: Muehlbauer, G., Li, X., Shin, S., Dill-Macky, R., Berthiller, F., Clemente, T., McCormick, S.P., Kumlehn, J., Adam, G. 2014. Using barley genomics to develop Fusarium head blight resistant wheat and barley [abstract]. American Barley Researchers Workshop.Interpretive Summary:
                                Technical Abstract: Fusarium head blight, caused by Fusarium graminearum, is a major problem for wheat and barley growers. During infection, F. graminearum produces trichothecene mycotoxins (e.g., deoxynivalenol or DON) that increases fungal virulence and reduces grain quality and yield. Previous work in Arabidopsis showed that an UDP-glucosyltransferases (DOGT1) inactivates DON by conjugating it with a glucoside group to DON-3-O-glucoside (D3G). Using genomics approaches we identified a barley UDP-glucosyltransferase (HvUGT13248) that exhibited DON resistance in yeast and Arabidopsis via conjugation of DON to D3G. Transgenic wheat in the Bobwhite and CBO37 backgrounds overexpressing HvUGT13248 exhibited high levels of type II resistance in the greenhouse and Type I resistance in the field compared to the nontransgenic controls. In addition, field grown transgenic plants compared to the nontransgenic controls exhibited a lower DON concentration. The mechanism of resistance in these transgenic plants appears to be the rapid conversion of DON to D3G.  Noteworthy, these plants also exhibited resistance to F. graminearum strains that produce the trichothecene nivalenol, indicating that HvUGT13248 has a broad spectrum of activity. Transgenic barley in the Golden Promise background overexpressing HvUGT13248 have been developed and we will present the screening results. To facilitate screening in the field, development of backcross lines in the Rasmusson background is in process.


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