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Title:   Author  item
                                            Bushakra, Jillitem
                                            BRYANT, DOUGLAS - Danforth Plant Science Centeritem
                                            DOSSETT, MICHAEL - Agriculture And Agri-Food Canadaitem
                                            VINING, KELLY - Oregon State Universityitem
                                            VANBUREN, ROBERT - Danforth Plant Science Centeritem
                                            Gilmore, Barbaraitem
                                            Lee, Jungminitem
                                            MOCKLER, TODD - Danforth Plant Science Centeritem
                                            Finn, Chaditem
                                            Bassil, Nahla
  Submitted to: Theoretical and Applied Genetics
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 5/18/2015
                                    Publication  Date: 6/22/2015
                                    Publication URL: http://link.springer.com/content/pdf/10.1007%2Fs00122-015-2541-x.pdfCitation: Bushakra, J., Bryant, D.W., Dossett, M., Vining, K.J., Vanburen, R., Gilmore, B.S., Lee, J., Mockler, T.C., Finn, C.E., Bassil, N.V. 2015. A genetic linkage map of black raspberry (Rubus occidentalis) and the mapping of Ag4 conferring resistance to the aphid Amphorophora agathonica. Theoretical and Applied Genetics. 128:1631-1646.Interpretive Summary: Black raspberry is a high-value crop in the Pacific Northwest of North America with an international marketplace. Few genetic resources are readily available and little improvement has been achieved through breeding efforts to address production challenges involved in growing this crop. Contributing to its lack of improvement is low genetic diversity in elite cultivars and an untapped reservoir of genetic diversity from wild germplasm. In the Pacific Northwest, where most production is centered, the current standard commercial cultivar is highly susceptible to the aphid, which is a vector for the Raspberry mosaic virus complex. Infection with the virus complex leads to a rapid decline in plant health resulting in field replacement after only 3-4 growing seasons. Sources of aphid resistance have been identified in wild germplasm and used to develop mapping populations to study the inheritance of these valuable traits. We have identified a region of the black raspberry genome that is associated with resistance to aphids. This is the first example of aphid resistance identified in this crop.
                                Technical Abstract: Black raspberry (Rubus occidentalis L.) is a high-value crop in the Pacific Northwest of North America with an international marketplace. Few genetic resources are readily available and little improvement has been achieved through breeding efforts to address production challenges involved in growing this crop. Contributing to its lack of improvement is low genetic diversity in elite cultivars and an untapped reservoir of genetic diversity from wild germplasm. In the Pacific Northwest, where most production is centered, the current standard commercial cultivar is highly susceptible to the aphid Amphorophora agathonica Hottes, which is a vector for the Raspberry mosaic virus complex. Infection with the virus complex leads to a rapid decline in plant health resulting in field replacement after only 3-4 growing seasons. Sources of aphid resistance have been identified in wild germplasm and used to develop mapping populations to study the inheritance of these valuable traits. We have constructed a genetic linkage map using single nucleotide polymorphism (SNP) and transferable (primarily simple sequence repeat, SSR) markers for F1 population ORUS 4305 consisting of 115 progeny that segregate for aphid resistance. Our linkage map of seven linkage groups representing the seven haploid chromosomes of black raspberry consists of 274 markers on the maternal map and 292 markers on the paternal map including a morphological locus for aphid resistance. This is the first linkage map of black raspberry and will aid in developing markers for marker assisted breeding, comparative mapping with other Rubus species, and enhancing the black raspberry genome assembly.


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