U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Vegetable Research: Charleston, SC
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Cucurbit Genetics CooperativeSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Southeast Area Charleston, South Carolina Vegetable Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Branham, Sandraitem
                                            Couillard, Daviditem
                                            Stansell, Zacharyitem
                                            Farnham, Mark
  Submitted to: Plant and Animal Genome Conference
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 12/15/2015
                                    Publication  Date: 1/11/2016
                                    Citation: Branham, S., Couillard, D.M., Stansell, Z.J., Farnham, M.W. 2016. Quantitative trait loci mapping of heat tolerance in a doubled haploid population of broccoli using genotyping-by-sequencing. Plant and Animal Genome Conference. P0950.Interpretive Summary: N/A
                                Technical Abstract: Broccoli is a cool weather vegetable crop with a vernalization requirement to initiate and maintain floral development. Breeding for heat tolerance in broccoli has the potential to both expand viable production areas and extend the growing season.  A doubled haploid (DH) population of broccoli (Brassica oleracea var. italica L.) consisting of 159 individuals segregating for heat tolerance was produced by a cross between heat-tolerant and heat-sensitive breeding lines. The population was evaluated for resistance to heat stress (using a 9 point scale) in 3 years of field trials in Charleston, SC. Population tolerance scores displayed a continuous distribution with a mean of 3.4 points and a standard deviation of 1.5 points. Genotyping-by-sequencing followed by imputation with FSFHap yielded 2,335 non-redundant informative single nucleotide polymorphisms (SNPs) for genetic map construction. Composite interval mapping was performed using the quantitiative trait loci (QTL) package in R. Identified QTL and their implications for marker-assisted selection in future breeding efforts will be discussed.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov