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Title:   Author  item
                                            KIM, WOO - US Department Of Agriculture (USDA)item
                                            Lillehoj, Hyunitem
                                            JEONG, MISUN - US Department Of Agriculture (USDA)item
                                            MIN, WONGI - Gyeongsang National Universityitem
                                            SULIVAN, YVONNE - Kingfisher Biotech, Incitem
                                            KAKACH, LINDA - Kingfisher Biotech, Incitem
                                            LABRESH, JOANNA - Kingfisher Biotech, Inc
  Submitted to: American Association for Immunology
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 12/3/2015
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: Interleukin-8/CXCL8 (IL-8) is a CXC-family chemokine produced by fibroblasts and other cell types, including epithelial cells, endothelial cells, neutrophils, and macrophages.  Given that IL-8 attracts lymphocytes to the sites of tissue damage, IL-8 plays a role in the inflammatory response and wound healing.  Previously, chicken chemotactic and angiogenic factor which is encoded by the 9E3/CEF4 gene, was identified and has been studied as a human IL-8 homologue.  However, detecting and quantifying this bioactive chemokine/cytokine in avian species is limited by the lack of specific antibodies and reliable bioassays.  Thus, we developed and characterized mouse monoclonal antibodies (mAbs) against chicken IL-8 to further define its immunological and biological properties.  Two mouse mAbs against chicken IL-8 were generated using recombinant IL-8 protein produced in Escherichia coli.  The specificities of these antibodies were confirmed by their specific binding with not only recombinant IL-8, but also native IL-8 by Western blot analysis, enzyme-linked immunosorbent assay, and immunocytochemistry.  Inhibition of IL-8-induced chemotaxis of peripheral blood lymphocytes, proliferation of chicken macrophage cells and expression of alpha smooth-muscle actin in chicken embryonic fibroblast cells by these mAbs indicate that both antibodies are capable of blocking IL-8 bioactivity.   Therefore, this study demonstrates that chicken immune reagents, which detect IL-8, will be valuable for basic as well as applied studies in poultry.


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