U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Wheat, Sorghum and Forage Research: Lincoln, NE
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Plains Area Lincoln, Nebraska Wheat, Sorghum and Forage Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Funnell-Harris, Deannaitem
                                            Sattler, Scottitem
                                            Graybosch, Robert
  Submitted to: National Fusarium Head Blight Forum
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 12/7/2014
                                    Publication  Date: 12/7/2014
                                    Citation: Funnell-Harris, D.L., Sattler, S.E., Graybosch, R.A. 2014. Presence of Fusarium graminearum in air associated with sorghum fields. National Fusarium Head Blight Forum Proceedings. p.63.Interpretive Summary:
                                Technical Abstract: Sorghum can be included in crop rotations with wheat. However, there are no known reports on the effects of sorghum grown in rotation with wheat on the epidemiology of head scab caused by Fusarium graminearum. Conidia in air samples within two sorghum fields were collected by passive spore trapping for two years at four plant stages (vegetative, anthesis, grain development or maturity) during the sorghum growing season. Spores were collected and germinated on a general fungal medium (potato dextrose agar; PDA) and a medium semi-selective for Fusarium spp. (pentachloronitrobenzene-containing agar; PCNB). Colonies cm-2 hr-1 on PCNB ranged from 31.0 to 85.7 percent of colonies cm-2 hr-1 on PDA, depending on environment and growth stage. A subsample of Fusarium isolates from PCNB traps were identified molecularly by comparing sequences from a portion of the translation elongation factor (TEF) 1a gene with those in the FUSARIUM-ID database (http://isolate.fusariumdb.org/index.php). Surprisingly, 26.8% were F. graminearum, the most numerous Fusarium species or genotype. Phylogenetic analyses of these isolates, as well as F. graminearum from sorghum leaf tissue and grain, using TEF, the rRNA internal transcribed spacer region and a portion of the histone-3 gene (H3), revealed that these isolates were highly similar to one another and to previously characterized F. graminearum isolates. Further research to determine whether isolates associated with sorghum production produce tricothecenes or zearalenone and are pathogenic to wheat, will need to be conducted to determine whether F. graminearum associated with sorghum production can affect head scab levels in wheat.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov