U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Bio-oils Research: Peoria, IL
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    NCAUR Homepage
                    BOR-NCAUR pilot plant facilities
                        Seed pressing and refining equipment
                                    Oil synthesis equipment
                                    Other equipmentSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area Peoria, Illinois National Center for Agricultural Utilization Research Bio-oils Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Oblath, Emilyitem
                                            Isbell, Terryitem
                                            Berhow, Markitem
                                            Allen, Brettitem
                                            Archer, Daviditem
                                            BROWN, JACK - University Of Idahoitem
                                            Gesch, Russell

                                                         - *Russ*item
                                            Hatfield, Jerryitem
                                            Jabro, Jalal

                                                         - *Jay*item
                                            Kiniry, Jamesitem
                                            Long, Daniel
  Submitted to: Industrial Crops and Products
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 3/9/2016
                                    Publication  Date: 5/17/2016
                                    Publication URL: https://handle.nal.usda.gov/10113/62886Citation: Oblath, E.A., Isbell, T.A., Berhow, M.A., Allen, B., Archer, D., Brown, J., Gesch, R.W., Hatfield, J.L., Jabro, J.D., Kiniry, J.R., Long, D.S. 2016. Development of near-infrared spectroscopy calibrations to measure quality characteristics in intact Brassicaceae germplasm. Industrial Crops and Products. 89:52-58.Interpretive Summary: Rapeseed, along with other oilseeds from the mustard family, is a potential feedstock for biofuel production but minor seed components can cause poor oil quality and increased refining costs. A rapid screening method using near-infrared spectroscopy was developed for six oilseed species from the mustard family. Intact seeds can be quickly analyzed for characteristics of interest including moisture, oil, fatty acid profile, nitrogen, glucosinolate, and chlorophyll content. Traditional analysis methods for many of these characteristics can be time-consuming and require large amounts of seeds to be destroyed. This rapid screening method allows samples to be non-destructively scanned in less than two minutes each. This is particularly useful for situations when only a small amount of seed is available, such as screening large numbers of samples from genetic trials to choose the best varieties for future research.
                                Technical Abstract: Determining seed quality parameters is an integral part of cultivar improvement and germplasm screening. However, quality tests are often time cnsuming, seed destructive, and can require large seed samples. This study describes the development of near-infrared spectroscopy (NIRS) calibrations to measure moisture, oil, fatty acid profile, nitrogen, glucosinolate, and chlorophyll content in six species from the Brassicaceae family. Rapeseed and similar oilseeds are potential feedstocks for producing hydrotreated renewable jet fuel. Screening samples with NIRS would allow cultivars with desirable characteristics to be quickly identified. A total of 367 samples of six species (Brassica napus, Brassica carinata, Brassica juncea, Brassica rapa, Sinapis alba, and Camelina sativa) were scanned with NIRS. Global calibrations for all six species were developed using modified partial least squares regression with reference values obtained through wet chemistry techniques. Comparing predicted values to reference data, the coefficients of determination (r2) and ratios of performance to deviation (RPD) varied, with some calibrations performing better than others. The calibration equations for seed oil content (r2 = 0.98, RPD = 7.3) and nitrogen (r2 = 0.98, RPD = 5.3) performed very well while the equations for moisture (r2 = 0.98, RPD = 5.3) performed very well while the equations for seed moisture (r2 = 0.98, RPD = 3.8) and glucosinolate content (r2 = 0.92, RPD = 2.3) were more qualitative. Large variation was observed for chlorophyll content (0-390 mg/kg) so two calibration equations were developed, one for the higher and one for the lower range of values. When combined, these calibrations also showed very good performance (r2 = 0.99, RPD = 14). The performance of the calibrations for the fatty acids was more varied, with some performing very well, such as the calibration for C18:3 (r2 = 0.99, RPD = 9.9), and others, such as C22:0 (r2 = 0.69, RPD = 1.9), showing poor correlation.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov