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Title:   Author  item
                                            XU, LINGYANG - University Of Marylanditem
                                            HE, YANGHUA - University Of Marylanditem
                                            DING, YI - University Of Marylanditem
                                            SUN, GUIRONG - University Of Marylanditem
                                            CARRILLO, JOSE ADRIAN - University Of Marylanditem
                                            LI, YAOKUN - University Of Marylanditem
                                            GHALY, MONA - University Of Marylanditem
                                            MA, LI - University Of Marylanditem
                                            Zhang, Huanminitem
                                            Liu, Ge

                                                         - *George*item
                                            SONG, JIUZHOU - University Of Maryland
  Submitted to: International Journal of Molecular Sciences
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 5/4/2017
                                    Publication  Date: 5/9/2017
                                    Publication URL: https://handle.nal.usda.gov/10113/5700719Citation: Xu, L., He, Y., Ding, Y., Sun, G., Carrillo, J., Li, Y., Ghaly, M.M., Ma, L., Zhang, H., Liu, G., Song, J. 2017. Characterization of copy number variation's potential Role in Marek’s disease. International Journal of Molecular Sciences. Available:http://wwww.mdpi.com/1422-0067/18/5/1020/pdf.Interpretive Summary: Marek's disease (MD) is a highly contagious disease of chickens, which costs the world poultry industry about 2 billion dollars each year. Industrial control of the disease is considered basically successful by routine vaccination and enhanced management measures. To improve the current control measures, genetic resistance to MD has been actively investigated to advance the understanding on genetics and epigenetics knowledge in relation to MD. This study was designed to identify the so-called copy number variation, also known as CNV, which refers to number of copies of a particular gene varying from one chicken to the next, in association with MD. Using the unique genetic resources of inbred chicken lines developed and being maintained at the USDA-ARS research lab at East Lansing, Michigan, a total of 393 CNVs were identified. Two particular CNVs are believed to confer MD based on the molecular genetics analyses. This finding may facilitate subsequent research projects to gain implementable measures for better control of the disease in chickens.
                                Technical Abstract: Marek's Disease (MD) is a highly contagious pathogenic and oncogenic disease primarily
affecting chickens. Chicken Lines 63 and 72 as well as their recombinant congenic strains (RCS) with varied susceptibility to MD are ideal models to study the complex mechanisms of genetic resistance to MD. In this study, we investigated copy number variations (CNV) in these inbred chicken lines using the 600 K Affymetrix Axiom HD SNP genotyping array. We detected 393 CNV segments across all ten chicken lines, of which 12 CNVs were specifically identified in Line 72. We then assessed genetic structure based on CNV and observed markedly different patterns. Finally, we validated two deletion events in Line 72 and correlated them with genes expression using qPCR and RNA-seq, respectively. Our combined results indicated that these two CNV deletions were likely to contribute to MD resistance/susceptibility.


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