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Title:   Author  item
                                            Dunlap, Christopheritem
                                            Bowman, Michaelitem
                                            Schisler, David
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 1/11/2015
                                    Publication  Date: 1/13/2015
                                    Citation: Dunlap, C.A., Bowman, M.J., Schisler, D.A. 2015. Discovery of secondary metabolites from Bacillus spp. biocontrol strains using genome mining and mass spectroscopy. Meeting Abstract. 179.Interpretive Summary:
                                Technical Abstract: Genome sequencing, data mining and mass spectrometry were used to identify secondary metabolites produced by several Bacillus spp. biocontrol strains. These biocontrol strains have shown promise in managing Fusarium head blight in wheat. Draft genomes were produced and screened in silico using genome mining software. Putative synthesis clusters were confirmed to be functional and the metabolites were structurally characterized with MS/MS techniques. We identified several known antifungal metabolites as well as antibiotic compounds which have limited efficacy information available. In addition, we documented the presence of several different siderophores, which have been associated with biocontrol activity. This study highlights the advantages of combining these techniques to rapidly characterize new biocontrol strains.


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