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Title:   Author  item
                                            PAULA, EDUARDO - University Of Nevada School Of Medicineitem
                                            DANES, MARINA - Universidade Federal De Lavrasitem
                                            LOBOS, NELSON - Kemin Industries, Incitem
                                            DRAGO, FERNANDO - Universidad De Sao Pauloitem
                                            Zanton, Geoffreyitem
                                            BRODERICK, GLEN - Retired ARS Employeeitem
                                            FACIOLA, ANTONIO - University Of Nevada School Of Medicine
  Submitted to: American Dairy Science Association Abstracts
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 4/21/2016
                                    Publication  Date: 7/8/2016
                                    Citation: Paula, E.M., Danes, M.A., Lobos, N.E., Drago, F.L., Zanton, G.I., Broderick, G., Faciola, A.P. 2016. Effects of replacing soybean meal with canola meal or treated canola meal on ruminal digestion, and omasal nutrient flow in lactating dairy cows [abstract]. Annual Meeting of American Dairy Science Association, July 19-23, 2016, Salt Lake City, Utah.Interpretive Summary:
                                Technical Abstract: Treated canola meal (TCM) was produced as an attempt to increase the rumen undegradable protein (RUP) fraction of canola meal (CM) with the goal of enhancing amino acid (AA) availability for absorption in the small intestine of dairy cows. The objective of this study was to measure nutrient and microbial omasal flow when CM and TCM replaced soybean meal (SBM) in the diet of dairy cows. Six cows fitted with ruminal cannulae were blocked into 2 blocks of 3 cows and randomly assigned within blocks to 3 dietary treatments in a 3x3 replicated Latin square design with 21 days of adaptation and 7 days of sampling. Treatments differed only in crude protein (CP) source, those sources being SBM, CM, and TCM. The TCM was treated by extrusion, with molasses added to promote the browning reaction. All diets contained (dry matter basis) 30% alfalfa silage, 30% corn silage, 4% soy hulls, 2.4% mineral-vitamin premix and 16% CP. The SBM diet contained 25% high-moisture corn (HMC) and 8.6% SBM; the canola diets contained 22% HMC and 11.4% CM or TCM. Omasal sampling was performed during the last week of each period. Data were analyzed using the MIXED procedure of SAS. Orthogonal contrasts were used to compare effects of different protein sources (SBM vs. CM + TCM) and (CM vs. TCM). Compared to SBM, CM and TCM increased N intake (P = 0.04). There was a trend (P =0.10) to increase rumen degradable protein (RDP) supply (kg/d), and N truly digested in the rumen (kg/d). There were no differences in dry matter intake, ruminal digestibility, efficiency of ruminal microbial synthesis, and flows of: RUP, dietary nutrients, non-microbial-non-ammonia-N, and total microbial-non-ammonia-N among diets. Results indicated that both canola diets may increase N intake and RDP supply. Treating CM by extrusion did not affect microbial N flow at the omasal canal. Under the conditions of the present study, treating CM by extrusion was not effective in increasing RUP flow in dairy cows.


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