U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Food and Feed Safety Research: College Station, TX
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Plains Area College Station, Texas Southern Plains Agricultural Research Center Food and Feed Safety Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Anderson, Robinitem
                                            LEVENT, GIZEM - Istanbul Universityitem
                                            He, Louisitem
                                            Genovese, Kenneth

                                                         - *Ken*item
                                            CIFTCIOGLU, GURHAN - Istanbul Universityitem
                                            Beier, Rossitem
                                            Hume, Michaelitem
                                            Harvey, Rogeritem
                                            Nisbet, David
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 5/22/2015
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: The gut of food-producing animals is a reservoir for human foodborne pathogens.  Thymol is bactericidal against pathogens including Salmonella and E. coli, but its rapid absorption from the proximal gut reveals a need for protective technologies to deliver effective concentrations to the lower gut where the pathogens mainly colonize.  Thymol-ß-D-glucopyranoside is more resistant to absorption than free thymol in everted jejunal segments and because of its ß-glycosidic bond could function as a prebiotic, being undegradable in the proximal gut but hydrolysable by microbial ß-glycosidases in the distal gut.  This study was conducted to determine if oral administration of thymol-ß-D-glucopyranoside could reduce gut colonization of Salmonella and E. coli in pigs.  Eighteen weaned pigs (24±5 kg live body weight) were orally infected with 2x10^9 CFU of a novobiocin and naladixic acid resistant Salmonella Typhimurium upon arrival (11:00) to the rearing facility and randomly allocated to 6 pens (3 pigs/pen) and twice-treated via oral gavage (2 pens/treatment) that same day (16:00 and 21:00) with 0, 1X, or 3X thymol-ß-D-glucopyranoside (x=6 mg/kg body weight).  Pigs were euthanized 12 h after the last treatment, and cecal and rectal contents collected at necropsy were diluted and plated to MacConkey agar for enumeration of generic E. coli and to Brilliant Green agar supplemented with 25 and 20 μg novobiocin and naladixic acid/mL, respectively, for enumeration of the challenge Salmonella.  Counts were log transformed and analyzed for effects of treatment using a 2x3 factorial design.  Cecal, but not rectal, concentrations of the challenge Salmonella were reduced in a dose dependent fashion by treatment (P<0.05), averaging (±SD) 3.5±1.1, 3.2±1.2, and 2.4±1.1 log10 CFU/g in cecal contents and 3.6±1.6, 3.0±1.3, and 2.8±1.3 log10 CFU/g from rectal contents.  The lack of a significant effect of thymol-ß-D-glucopyranoside on rectal Salmonella suggests that hydrolysis of ß-glycosidic bond may have been too rapid to allow its escape from the cecum.  Neither cecal nor rectal concentrations of wildtype E. coli were affected by treatment (P>0.05), averaging 6.1±0.7 and 7.0±0.7 log10 CFU/g, respectively.  The lack of an effect of thymol-ß-D-glucopyranoside on cecal or rectal E. coli concentrations is consistent with observations from an earlier study where Campylobacter jejuni but not E. coli was significantly reduced in the crop of broilers orally-treated with thymol-ß-D-glucopyranoside.  Similarly, in vitro studies have indicated that E. coli may be less sensitive to thymol than some other bacteria.  These results provide evidence that thymol-ß-D-glucopyranoside may be an effective prebiotic to bypass absorption and degradation in proximal gut but further studies are needed to optimize dosages and administration procedures to yield a practical and economically viable feed additive to reduce carriage of important foodborne pathogens in food-producing animals.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov