U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Plant Stress and Germplasm Development Research: Lubbock, TX
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Projects
                        NP 301Search small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Plains Area Lubbock, Texas Cropping Systems Research Laboratory Plant Stress and Germplasm Development Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Xin, Zhanguoitem
                                            Chen, Junpingitem
                                            Burow, Gloriaitem
                                            Burke, John
  Submitted to: Book Chapter
                                    Publication Type: Book / Chapter
                                    Publication Acceptance Date: 5/1/2014
                                    Publication  Date: 7/8/2014
                                    Citation: Xin, Z., Chen, J., Burow, G.B., Burke, J.J. 2014. Phenotypic mutant library:  potential for gene discovery. In: Wang, Y-H, Upadhyaya, H.D., Kole, C., editors. Genetics, Genomics and Breeding of Sorghum. Boca Raton, FL: CRC Press. p. 284-302.Interpretive Summary:
                                Technical Abstract: The rapid development of high throughput and affordable Next-
Generation Sequencing (NGS) techniques has renewed interest in
gene discovery using forward genetics. The conventional forward
genetic approach starts with isolation of mutants with a phenotype
of interest, mapping the mutation within a small chromosomal region
through linkage analysis, and identifi cation of the gene that carries the expected mutation. Depending on the availability of polymorphic markers in the region around the mutation, it can take years to identify the causal mutation even in model plants, like Arabidopsis and rice.  New strategies based on NGS combine linkage analysis of hundred of thousand markers with the identifi cation of the causal gene into one step, greatly increase the effi ciency of gene discovery through forward genetics. Once the mapping populations segregating for the mutant phenotype are established, the causal gene can be identifi ed in a few weeks. With continuous increase in throughput and reduction in sequencing cost, mapping strategies based on NGS will play a major role in gene discovery from mutants with desired phenotypes. Thus, identification of relevant mutants and development of the populations segregating for mutant phenotype will become the limiting factor. We have developed a sorghum (Sorghum bicolor L. Moench) pedigreed mutant library in the inbred line BTx623, which is used for generating
the sorghum reference genome sequence. This mutant library displays 
a wide range of visible phenotype and can serve as a critical resource for rapid discovery of genes critical for yield, adaptation, nutritional quality and biofuel traits.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov