U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Crops Pathology and Genetics Research: Davis, CA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Outreach Events
                        UC Davis Picnic Day
                                    Kinders go to College
                                    STEM Girls
                                    Future Scientist Program
                                    Agriculture Future of America
                                    Wallace-Carver Fellowship
                                    Young Scholars Program
                                    Annual Awards Banquet
                    About Us
                        About Us
                                    Our Mission
                                    Principal InvestigatorsSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Pacific West Area Davis, California Crops Pathology and Genetics Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications
- Etiology and Biology of Tree Crop Diseases
- Rice Genetics
- Sustainable Floriculture
- Sustainable Viticulture

Title:   Author  item
                                            HENRY, ISABELLE - University Of Californiaitem
                                            NAGALAKSHMI, UGRAPPA - University Of Californiaitem
                                            LIEBERMAN, MERIC - University Of Californiaitem
                                            NGO, KATHIE - University Of Californiaitem
                                            KRASILEVA, KSENIA - University Of Californiaitem
                                            VASQUEZ-GROSS, HANS - University Of Californiaitem
                                            AKHUNOVA, ALINA - Kansas State Universityitem
                                            AKHUNOVA, EDUARD - Kansas State Universityitem
                                            DUBCOVSKY, JORGE - University Of Californiaitem
                                            Tai, Thomasitem
                                            COMAI, LUCA - University Of California
  Submitted to: The Plant Cell
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 3/19/2014
                                    Publication  Date: 4/11/2014
                                    Publication URL: http://www.plantcell.org/content/26/4/1382.abstract?sid=0256eb0a-81a1-458f-94ce-a9c358174ee2Citation: Henry, I.M., Nagalakshmi, U., Lieberman, M.C., Ngo, K.J., Krasileva, K.V., Vasquez-Gross, H., Akhunova, A., Akhunova, E., Dubcovsky, J., Tai, T., Comai, L. 2014. Efficient genome-wide detection and cataloging of EMS-induced mutations using exome capture and next-generation sequencing. The Plant Cell. 26:1-17.Interpretive Summary:
                                Technical Abstract: Chemical mutagenesis efficiently generates phenotypic variation in otherwise homogeneous genetic backgrounds, enabling functional analysis of genes. Advances in mutation detection have brought the utility of induced mutant populations on par with those produced by insertional mutagenesis, but systematic cataloguing of mutations would further increase their utility. We examined the suitability of multiplexed global exome capture and sequencing coupled with custom-developed bioinformatics tools to identify mutations in well-characterized mutant populations of rice (Oryza sativa) and wheat (Triticum aestivum). In rice, we identified 
~18,000 induced mutations from 72 independent M2 individuals. Functional evaluation indicated the recovery of potentially deleterious mutations for >2600 genes. We further observed that specific sequence and cytosine methylation patterns surrounding the targeted guanine residues strongly affect their probability to be alkylated by ethyl methanesulfonate. Application of these methods to six independent M2 lines of tetraploid wheat demonstrated that our bioinformatics pipeline is applicable to polyploids. In conclusion, we provide a method for developing large-scale induced mutation resources with relatively small investments that is applicable to resource-poor organisms. Furthermore, our results demonstrate that large libraries of sequenced mutations can be readily generated, providing enhanced opportunities to study gene function and assess the effect of sequence and chromatin context on mutations.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov