U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Crop Production and Pest Control Research: West Lafayette, IN
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area West Lafayette, Indiana Crop Production and Pest Control Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications
- Small Grains Viral Disease Laboratory
- Corn and Sorghum Fungal Disease Laboratory
- Host Plant Resistance to Insects Laboratory
- Wheat/Hessian fly Interactions Laboratory
- Small Grains Fungal Disease Laboratory
- Soybean Quality Improvement Laboratory
- Uniform Soybean Tests, Northern Region

Title:   Author  item
                                            GUNUPURU, LOKANADHA - University College Dublinitem
                                            ALI, SHAHIN - University College Dublinitem
                                            DOOHAN, FIONA - University College Dublinitem
                                            Scofield, Steven

                                                         - *Steve*
  Submitted to: Bio-protocol
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 6/20/2015
                                    Publication  Date: 6/20/2015
                                    Citation: Gunupuru, L.R., Ali, S.S., Doohan, F.M., Scofield, S.R. 2015. Virus-induced gene silencing (VIGS) in barley seedling leaves. Bio-protocol. 5(12): 1506-8.Interpretive Summary: Virus-induced gene silencing (VIGS) is an important experimental technique for establishing the function of plant genes.  Scientists can test the function of selected genes by assembling engineered plant viruses that cause the chosen genes to be inactivated when the VIGS virus infects plants.  The function of the gene can then be inferred from changes in the behavior of VIGS plants in comparison to control plants.  This paper reports procedures that can be used to silence genes in barley plants using VIGS constructs based on Barley stripe mosaic virus.  This procedure is of immediate interest to scientists investigating a wide range of biological questions in barley and other small grain cereal crops.  But, its wider impact will come in the improved crops that will result from the research this innovative technique enables.
                                Technical Abstract: Virus-induced gene silencing (VIGS) is one of the most potent reverse genetics technologies for gene functional characterization. This method exploits a dsRNA-mediated antiviral defense mechanism in plants. Using this method allows researchers to generate rapid phenotypic data in a relatively rapid time frame as compared to the generation of stable transformants. Here we describe a simple method for silencing a target gene in barley seedling leaves using vectors based on the Barley Stripe Mosaic Virus (BSMV).


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov