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Title:   Author  item
                                            Lillehoj, Hyunitem
                                            KIM, JI EUN - US Department Of Agriculture (USDA)item
                                            LIM, YAESEUL - US Department Of Agriculture (USDA)item
                                            JUNG, MISUN - US Department Of Agriculture (USDA)item
                                            SULLIVAN, YVONNE - Kingfisher Biotech, Incitem
                                            LEE, SUNG HYUN - US Department Of Agriculture (USDA)
  Submitted to: American Veterinary Medical Association Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 4/5/2015
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: Interleukin-8 (IL-8) is a key mediator in neutrophil-mediated acute proinflammatory response and activates multiple signaling pathways downstream of two receptors (CXCR1 and CXCR2).  However, there have not been any monoclonal antibodies which specifically detect chicken IL8 (chIL8) and define its biochemical and immunological properties.  Therefore, our laboratory characterized and developed three mouse monoclonal antibodies (mAbs) which detect chIL8 by ELISA, Western blot,and Luminex assay.  Using Western blot analysis, mAb specific for chIL8 identified a 9.5kDa Pichia pastgoris-expressed chIL8 and a 32kDa E.coli-derived, poly-histidine-tagged fusion protein of chIL8.  The mAbs were used to measure serum IL8 levels in normal and necrotic enteritis (NE)-afflicted chickens by antigen capture ELISA and blocked the cytotoxic effect of chIL8 against lymphoblastoid chicken B tumor cells (LSCC-RP9).  These new mAbs that detect chIL8 will be important immune reagents for basic and applied research in poultry immunology.


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