U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Dairy and Functional Foods Research: Wyndmoor, PA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    About ERRC
                        Greetings from the Director
                                    Directions/Lodging
                                    History and Accomplishments
                    Our Work
                        Biomass Pyrolysis Research
                                    IPMP 2013
                                    IPMP Dynamic Prediction
                                    IPMP Global Fit
                                    Pathogen Modeling Program (PMP)
                                    Predictive Microbiology Information Portal (PMIP)
                                    PoultryFARMSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Northeast Area Wyndmoor, Pennsylvania Eastern Regional Research Center Dairy and Functional Foods Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Paul, Moushumiitem
                                            Phillips, Johnitem
                                            Renye, John
  Submitted to: Journal of Dairy Science
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 11/20/2015
                                    Publication  Date: 5/1/2016
                                    Publication URL: http://handle.nal.usda.gov/10113/62621Citation: Paul, M., Phillips, J.G., Renye Jr, J.A. 2016. Short communication: Measuring the angiotensin-converting enzyme inhibitory activity of an 8-amino acid (8mer) fragment of the C12 antihypertensive peptide. Journal of Dairy Science. 99(5):3263-3266.  doi: 10.3168/jds.2015-10437.Interpretive Summary: Being able to increase the nutritional and health-promoting properties of food using milk-derived ingredients is a challenge that has attracted a great deal of attention recently. Many bioactive compounds derived from milk proteins exhibit potential as functional food ingredients aimed at combating various conditions that can be controlled through diet, such as cardiovascular disease, type II diabetes and obesity.  Previous research explored the possibility of incorporating a small peptide (C12 peptide) that helps reduce blood pressure into milk products such as yogurt.  It was found that it is broken down by the starter cultures used for fermentation into a smaller 8 amino acid (8mer) peptide that is stable under yogurt-making conditions.  This research addresses the question of whether the 8mer peptide retains the same biological activity exhibited by the parent C12 peptide. We have synthesized and purified the 8mer peptide in house and assessed its antihypertensive activity. Our results indicate that it does indeed retain biological activity and that it remains stable in the presence of starter cultures.  The 8mer peptide now has the potential to be used as a health-promoting functional ingredient in many fermented dairy foods.
                                Technical Abstract: An eight amino acid fragment (PFPEVFGK) of a known milk protein-derived antihypertensive peptide was synthesized by microwave-assisted solid phase peptide synthesis and purified by reverse phase HPLC.  Its ability to inhibit the angiotensin-converting enzyme was assessed and compared to that of the parent 12mer peptide (FFVAPFPEVFGK) to determine the effect of truncating the sequence on the overall hypotensive activity.  The activity of the truncated 8mer peptide was found to be almost 1.5 times less active than the 12mer, with IC50 values of 108 and 69 uM, respectively.  The overall activities of both the 12mer and the 8mer, however, are in the range of being physiologically important and potentially active following oral administration, and therefore may have potential use as a blood pressure lowering agent in humans in the future.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov