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Title:   Author  item
                                            Funnell-Harris, Deannaitem
                                            Sattler, Scottitem
                                            O`Neill, Patrickitem
                                            ESKRIDGE, KENT - University Of Nebraska
  Submitted to: American Phytopathological Society Annual Meeting
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 5/7/2015
                                    Publication  Date: 8/1/2015
                                    Publication URL: http://www.apsnet.org/meetings/Documents/2015_meeting_abstracts/aps2015abO186.htmCitation: Funnell-Harris, D.L., Sattler, S.E., Oneill, P.M., Eskridge, K.M. 2015. Effect of waxy (low amylose) on fungal infection of sorghum grain. American Phytopathological Society Annual Meeting. 76-0.Interpretive Summary:
                                Technical Abstract: Loss of function mutations in the Waxy (Wx) gene, encoding Granule Bound Starch Synthase (GBSS) that synthesizes amylose, results in starch granules containing mostly amylopectin. Grain with this trait has increased usability for feed, food and grain-based ethanol, due to altered starch properties. In sorghum, two classes of waxy (wx) alleles had been previously characterized for absence or presence of GBSS: wxa (GBSS-) and wxb (GBSS+, with reduced activity). Field-grown grain of wild-type; waxy, GBSS-; and waxy, GBSS+ Plant Introduction accessions, were screened for fungal infection. Overall, results showed that waxy grains were not more susceptible than wild-type. GBSS- and wild-type grain had similar infection levels. However, height was a factor with waxy, GBSS+ lines: wxb accessions, which were short, were more susceptible than tall waxy accessions, with an undescribed allele. In greenhouse experiments, grain from accessions and near-isogenic wxa, wxb and wild-type lines, were inoculated with Alternaria sp., Fusarium thapsinum and Curvularia sorghina, to analyze germination and seedling fitness. As a group, waxy lines were not more susceptible to grain pathogens than wild-type, supporting field evaluations. Amongst most waxy and wild-type lines, reduced emergence, survival and seedling weights were observed after C. sorghina and F. thapsinum inoculations. These results are valuable for developing waxy hybrids with resistance to grain-infecting fungi.


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