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ARS Home Midwest Area Ames, Iowa National Animal Disease Center Virus and Prion Research Research Publications at this Location





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Title:   Author  item
                                            SIEPKER, CHRIS - Kansas State Universityitem
                                            HALEY, NICHOLAS - Kansas State Universityitem
                                            WALTER, W. DAVID - Us Geological Survey (USGS)item
                                            HOON-HANKS, LAURA - Colorado State Universityitem
                                            MONELLO, RYAN - Us National Park Serviceitem
                                            POWERS, JENNY - Us National Park Serviceitem
                                            Greenlee, Justinitem
                                            THOMSEN, BRUCE - Animal And Plant Health Inspection Service (APHIS)item
                                            LEHMKUHL, AARON - Animal And Plant Health Inspection Service (APHIS)item
                                            MITCHELL, GORDON - Canadian Food Inspection Agencyitem
                                            NICHOLS, TRACY - Colorado State Universityitem
                                            HOOVER, EDWARD - Colorado State Universityitem
                                            RICHT, JUERGEN - Kansas State University
  Submitted to: Prion
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 3/5/2015
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: Chronic wasting disease (CWD) is an efficiently transmitted spongiform encephalopathy of cervids (e.g. deer, elk, and moose), and is the only known prion disease affecting both free-ranging wildlife and captive animals. The antemortem detection of CWD and other prion diseases has proven difficult, due in part to difficulties in identifying an appropriate peripheral tissue specimen and complications with conventional test sensitivity. At present, biopsies of the recto-anal mucosal-associated lymphoid tissues (RAMALT) have shown promising sensitivity and are practical to collect in live animals. Nasal brush collections have likewise proven both sensitive and practical for identification of prion infections in humans. In this study, we evaluated both RAMALT and nasal brush collections by real time quaking-induced conversion (RT-QuIC), and compared our findings to RAMALT immunohistochemistry as well as conventional postmortem evaluation of obex and retropharyngeal lymph node tissues from over 700 captive and free-ranging deer and elk in areas with endemic CWD. We correlated our results with various clinical findings, including pathological stage of infection as determined by obex scoring, PrP genotype, age, and sex. While the sensitivity of RAMALT RT-QuIC analyses exceeded that of RAMALT IHC (69-80% vs. >44%) and nasal brush collections (15-30%), the sensitivity of both biopsy and nasal brush analyses were dependent primarily on clinical stage of disease, although PrP genotype was also an important predictor of sample positivity. Our findings further demonstrate the potential and limitations of antemortem sample analyses by RT-QuIC in the identification and management of prion diseases.


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