U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Tropical Plant Genetic Resources and Disease Research: Hilo, HI
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Pacific West Area Hilo, Hawaii Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center Tropical Plant Genetic Resources and Disease Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            SANG-NGERN, MAYURAMAS - University Of Hawaiiitem
                                            YOUN, YI - University Of Hawaiiitem
                                            PARK, EUN-JUNG - University Of Hawaiiitem
                                            KONDRATYU, TAMARA - University Of Hawaiiitem
                                            SIMMONS, CHARLES - University Of Hawaiiitem
                                            LORCH, SAM - Lani Ko Honua Berry Farmitem
                                            Wall, Marisaitem
                                            PEZZUTO, JOHN - University Of Hawaiiitem
                                            CHANG, LENG CHEE - University Of Hawaii
  Submitted to: Bioorganic and Medicinal Chemistry Letters
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 4/25/2016
                                    Publication  Date: 6/15/2016
                                    Citation: Sang-Ngern, M., Youn, Y., Park, E., Kondratyu, T.P., Simmons, C.J., Lorch, S.E., Wall, M.M., Pezzuto, J.M., Chang, L. 2016. Withanolides derived from Physalis peruviana (Poha) with potential anti-inflammatory activity. Bioorganic and Medicinal Chemistry Letters. 26(12):2755-2759.Interpretive Summary: Physalis peruviana (Cape gooseberry, goldenberry, or Hawaiian poha) is used in traditional folk medicine for the treatment of cancer and other diseases.  Withanolides occur as secondary metabolites in Physalis, and have reputed anti-inflammatory, antitumor, cytotoxic, hepatotoxic, and antimicrobial activities. Three new withanolides, together with seven known compounds, were isolated and identified from P. peruviana plants grown in Hawaii. Their chemical structures and biological activities were elucidated. The isolated compounds were evaluated in anti-inflammatory bioassays, and four of the compounds exhibited high or moderate levels of activity. This natural products research furthers our understanding of the medicinal properties of poha and expands our cache of biologically active constituents from tropical plants.
                                Technical Abstract: Three new withanolides, physaperuvin G (1), physaperuvin I (2), physaperuvin J (3), along with seven known derivatives (4-10), were isolated from the aerial parts of Physalis peruviana. The structures of 1-3 were determined by spectroscopic methods, including, 1D and 2D NMR, and mass spectrometry. The absolute configuration of compound 1 was confirmed using X-ray diffraction method. Compounds 1-10 were evaluated for their inhibitory effects on nitric oxide (NO) production in lipopolysaccharide (LPS)-activated murine macrophage RAW 264.7 cells, and the tumor necrosis factor alpha (TNF-a)-activated nuclear factor-kappa B (NF-kB) pathway with transfected human embryonic kidney cells 293. Compounds 4, 5, 6, and 7 inhibited TNF-a-induced NF-kB activity with the half maximal inhibitory concentration (IC50) values of 0.04, 5.6, 2.1, and 1.1 uM, respectively; compounds 4,  5, and 10 exhibited NO inhibitory activity with IC50 values of 0.32, 2.4, and 7.8 uM respectively.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov