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ARS Home Pacific West Area Corvallis, Oregon National Clonal Germplasm Repository Research Publications at this Location





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Title:   Author  item
                                            Bushakra, Jillitem
                                            BRYANT, DOUG - Danforth Plant Science Centeritem
                                            DOSSETT, MICHAEL - Agriculture And Agri-Food Canadaitem
                                            Gilmore, Barbaraitem
                                            FILICHKIN, SERGEI - Oregon State Universityitem
                                            Weiland, Gerald

                                                         - *Jerry*item
                                            Peterson, Maryitem
                                            BRADISH, CHRISTINE - North Carolina State Universityitem
                                            FERNANDEZ, GINA - North Carolina State Universityitem
                                            Lewers, Kimberlyitem
                                            GRAHAM, JULIE - The James Hutton Instituteitem
                                            Lee, Jungminitem
                                            MOCKLER, TODD - Danforth Plant Science Centeritem
                                            Bassil, Nahlaitem
                                            Finn, Chad
  Submitted to: Plant Biology Annual Meeting
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 3/20/2014
                                    Publication  Date: 3/20/2014
                                    Citation: Bushakra, J., Bryant, D., Dossett, M., Gilmore, B.S., Filichkin, S., Weiland, G.E., Peterson, M.E., Bradish, C.M., Fernandez, G., Lewers, K.S., Graham, J., Lee, J., Mockler, T., Bassil, N.V., Finn, C.E. 2014. Black raspberry genetic and genomic resources development [abstract]. Annual Scientific Meeting of the American Society of Plant Biologists.Interpretive Summary: Since the early 1900s, the black raspberry industry in the United States has steadily declined due to lack of cultivars that are adapted to a range of environments and that have disease resistance. Interest in developing new cultivars has been fueled by by news regarding the potential health benefits of black raspberry compounds. We are developing methods to study the genetics of black raspberry that will benefit both black and red raspberry U.S. breeding programs. We used a new method to determine the genetic diversity of a black raspberry population. Using this new method, we detected differences that will aid in determining the inheritance of important horticultural traits such as resistance to feeding aphids and fungal diseases, and plant growth characteristics.
                                Technical Abstract: This study incorporates field and laboratory components to advance and streamline identification of a variety of traits of economic interest and to develop molecular markers for marker assisted breeding of black raspberry (Rubus occidentalis). A lack of adapted, disease resistant cultivars has led to a steady decline of the black raspberry (BR) industry in the US. Interest in production and breeding new cultivars has been fueled by news regarding the potential health benefits of black raspberry bioactive compounds. To accomplish our goals, we are using two related full-sibling populations that have been replicated and planted at five production sites (OR, WA, OH, NC, NY). We are taking detailed measurements on traits including primocane vigor, flowering and fruiting, disease and aphid tolerance, and plant architecture to assess the influence of environment on genotype (GxE). Initial analysis of (GxE) on primocane vigor indicated that individual genotypes showed significant variation among sites. We are also developing, and making available, genomic tools including molecular markers for construction of linkage and physical maps, and a draft genome assembly. Markers are being developed by sequencing and analyzing libraries generated by genotyping by sequencing (GBS). Initial analysis through a custom data pipeline identified over 23,000 single nucleotide polymorphic/insertion-deletion (SNP/indel) loci. Preliminary results indicate that GBS is appropriate for SNP detection in this highly-homozygous species. A densely populated genetic linkage map will be used to improve the draft genome assembly, for quantitative trait locus (QTL) mapping, and comparative genomic studies with other Rosaceae.


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