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Title:   Author  item
                                            Lillehoj, Hyunitem
                                            JANG, SEUNG - US Department Of Agriculture (USDA)item
                                            LEE, SUNG-HYEN - US Department Of Agriculture (USDA)item
                                            LEE, KYUNG WOO - US Department Of Agriculture (USDA)item
                                            HONG, YEONG HO - US Department Of Agriculture (USDA)item
                                            DUPUIS, LAURENT - Seppic, Incitem
                                            BEN AROUS, JULIETTE - Seppic, Inc
  Submitted to: American Veterinary Medical Association Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 4/5/2015
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: The current study was conducted to investigate the effects of in ovo injection of recombinant clostridium NetB toxin plus Eimeria profilin proteins in combination with Montanide adjuvants in modulating the immune system in chickens infected for experimental necrotic enteritis (NE) disease.  Broiler eggs were injected with 100 μl of PBS, profilin, profilin plus NetB, profilin plus NetB/Montanide IMS OVO 1 adjuvant (OVO1), or profilin plus NetB/Montanide IMS OVO 2 adjuvant (OVO2).  Intestinal lesion scores, body weight gain, Net-B toxin antibody levels, and proinflammatory cytokine and chemokine levels were measured as outcomes of protection following oral co-infection with C. perfringens and Eimeria maxima.  Birds in ovo vaccinated with recombinant profilin plus NetB proteins/OVO1 and recombinant profilin plus NetB proteins/OVO2 showed significantly increased body weight gain and reduced gut lesions compared with the profilin-only group, respectively. Greater NetB toxin antibody titers were observed in the profilin plus NetB/OVO1, and profilin plus NetB/OVO2 groups compared with the other three vaccine groups.  Finally, decreased levels of gene transcripts encoding interleukin-8, tumor necrosis factor superfamily 15, and TNF-a factor were observed in the intestinal lymphocytes of chickens in ovo injected with profilin plus NetB toxin in combination with OVO1 or  profilin plus NetB toxin in combination with OVO2 compared with profilin protein.  These results suggest that in ovo injection with recombinant profilin plus NetB proteins in combination with Montanide IMS adjuvants enhances protective immunity against experimental NE disease.


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