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Title:   Author  item
                                            KIM, WOO - US Department Of Agriculture (USDA)item
                                            Lillehoj, Hyunitem
                                            JEONG, MISUN - US Department Of Agriculture (USDA)item
                                            SULIVAN, YVONNE - Kingfisher Biotech, Incitem
                                            KAKACH, LAURA - Kingfisher Biotech, Incitem
                                            LABRESH, JOANNA - Kingfisher Biotech, Inc
  Submitted to: Poultry Science Association Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 12/3/2015
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: Limited information on chicken cytokines and chemokines hinders progress in understaidng  the role of cell-mediated immunity in infections. Interleukin-8/CXCL8 (IL-8) is a CXC-family chemokine produced by fibroblasts and other cell types, including epithelial cells, endothelial cells, neutrophils, and macrophages.  IL-8 attracts lymphocytes to sites of tissue damage, and plays a role in the inflammatory response and wound healing.  The chicken chemotactic and angiogenic factor, encoded by the 9E3/CEF4 gene, was identified and has been studied as a human IL-8 homologue for over more than 20 years.  However, the detecting and quantifying of the bioactive avian specific IL-8 chemokine/cytokine is limited by the lack of specific antibodies and reliable bioassays although the use of monoclonal antibodies (mAbs) has become routine in the mammalian research and diagnosis.  Thus, we developed and characterized mouse mAbs against chIL-8 to further define its immunological and biological properties.  Two mouse mAbs against IL-8 were generated using recombinant chicken IL-8 protein produced in Escherichia coli.  These mAbs were confirmed to show specific binding with not only recombinant IL-8, but also the native IL-8 protein by Western blot analysis, enzyme-linked immunosorbent assay, and immunocytochemistry.  Inhibition of IL-8-induced chemotaxis of peripheral blood lymphocytes, proliferation of chicken macrophage cells and expression of alpha smooth-muscle actin in chicken embryonic fibroblast cells by both antibodies indicate that both antibodies are capable of blocking chIL-8 bioactivity.  These results show that two mAbs have been generated that detect native IL-8 for basic immunology studies in poultry.


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