U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Obesity and Metabolism Research: Davis, CA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Laboratories
                        Bennett Group
                                    Huang Group
                                    Keim Group
                                    Laugero Group
                                    Newman GroupSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Pacific West Area Davis, California Western Human Nutrition Research Center Obesity and Metabolism Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            LA MERRILL, MICHELLE - University Of Californiaitem
                                            KAREY, EMMA - University Of Californiaitem
                                            MOSHIER, ERIN - Mount Sinai School Of Medicineitem
                                            LINDTNER, CLAUDIA - Mount Sinai School Of Medicineitem
                                            LA FRANO, MICHAEL - University Of Californiaitem
                                            Newman, Johnitem
                                            BUETTNER, CHRISTOPH - Mount Sinai School Of Medicine
  Submitted to: PLOS ONE
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 6/26/2014
                                    Publication  Date: 7/30/2014
                                    Citation: La Merrill, M., Karey, E., Moshier, E., Lindtner, C., La Frano, M., Newman, J.W., Buettner, C. 2014. Perinatal exposure to the pesticide DDT impairs energy expenditure and metabolism in adult female mice. PLoS One. 9(7):e103337. doi: 10.1371/journal.pone.0103337.Interpretive Summary: The insecticide dichlorodiphenyltrichloroethane (DDT) had been used extensively to control malaria, typhus, body lice and bubonic plague worldwide, until countries began restricting its use in the 1970s.  Individuals exposed to elevated levels of DDT and its metabolites have an increased prevalence of diabetes and insulin resistance. We hypothesize that DDT exposure of mice during the preinatal period, i.e. the period of time before and after birth, would disrupt metabolic programming leading to impaired metabolism in adult offspring. To test this, we administered DDT to C57BL/6J mice from gestational day 11.5 (equivalent to a ~3rd week of human pregnancy) to postnatal day 5 (equivalent to a ~6 month human infant) and studied their metabolic phenotype at several ages up to nine months (equivalent to a 25 year old human). Perinatal DDT exposure reduced core body temperature, impaired cold tolerance, decreased energy expenditure, and produced a transient early-life increase in the body fat of females. When challenged with a high fat diet for 12 weeks in adulthood, female offspring with perinatal DDT exposure developed glucose intolerance, hyperinsulinemia, dyslipidemia, altered bile acid metabolism and further thermogeneic impairment. Perinatal DDT exposure impaired RNA associated with substrate utilization and thermogenesis in the brown adipose of 9 month old female mice. These observations suggest that perinatal DDT exposure impairs thermogenesis, carbohydrate-, and lipid- metabolism and may either cause metabolic syndrome in adult offspring or increase their susceptibility to this disorder.
                                Technical Abstract: Dichlorodiphenyltrichloroethane (DDT) has been used extensively to control malaria, typhus, body lice and bubonic plague worldwide, until countries began restricting its use in the 1970s. Individuals exposed to elevated levels of DDT and its metabolite dichlorodiphenyldichloroethylene have an increased prevalence of diabetes and insulin resistance. Here we hypothesize that perinatal exposure to DDT disrupts metabolic programming leading to impaired metabolism in adult offspring. To test this, we administered DDT to C57BL/6J mice from gestational day 11.5 to postnatal day 5 and studied their metabolic phenotype at several ages up to nine months. Perinatal DDT exposure reduced core body temperature, impaired cold tolerance, decreased energy expenditure, and produced a transient early-life increase in body fat in female offspring. When challenged with a high fat diet for 12 weeks in adulthood, female offspring with perinatal DDT exposure developed glucose intolerance, hyperinsulinemia, dyslipidemia, altered bile acid metabolism and further impaired thermogenesis. Perinatal DDT exposure impaired RNA associated with substrate utilization and thermogenesis in the brown adipose of 9 month old female mice. These observations suggest that perinatal DDT exposure impairs thermogenesis, carbohydrate-, and lipid- metabolism and may cause metabolic syndrome in adult offspring.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov