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ARS Home Midwest Area Peoria, Illinois National Center for Agricultural Utilization Research Functional Foods Research Research Publications at this Location





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Title:   Author  item
                                            Berhow, Markitem
                                            Bowman, Michael
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 10/4/2014
                                    Publication  Date: 10/4/2014
                                    Citation: Berhow, M.A., Bowman, M.J. 2014. Collision-induced fragmentation accurate mass spectrometric analysis methods to rapidly characterize plant extracts [abstract]. Illinois Heartland Local Section of the American Chemical Society.Interpretive Summary:
                                Technical Abstract: The rapid advances in analytical chromatography equipment have made the reliable and reproducible measurement of a wide range of plant chemical components possible. Full chemical characterization of a given plant material is possible with the new mass spectrometers currently available. For phytochemicals, we have used the latest accurate mass LC-MS analysis to quickly characterize the potential chemical formulas for a series of unknown compounds in a variety of plant extracts using a standardized CID/HCD mass fragmentation experiment in which interpretation of daughter ions produced allowed for the identification of a variety of phytochemical constituents. These results – coupled with DEPT NMR analysis as needed – resulted in the identification of a series of phytochemicals in camelina and sorghum pith extracts. Accurate LC-MSN screening and characterization analyses can be coupled to more rapid non-destructive spectrophotometric analytical methods such as pulsed NMR and near infrared (NIR) spectrometry, which will allow for the rapid and non-destructive analysis of thousands of samples for a wide range of chemical composition parameters.


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