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Title:   Author  item
                                            MCKENNA, DUANE - University Of Memphisitem
                                            Scully, Erinitem
                                            PAUCHET, YANNICK - Max Planck Societyitem
                                            HOOVER, KELLI - Pennsylvania State Universityitem
                                            KIRSCH, ROY - Max Planck Societyitem
                                            Geib, Scottitem
                                            MITCHELL, ROBERT - University Of Arizonaitem
                                            WATERHOUSE, ROBERT - Massachusetts Institute Of Technologyitem
                                            AHN, SEUNG-JOON - Max Planck Societyitem
                                            ARSALA, DEANNA - University Of Illinoisitem
                                            Childers, Annaitem
                                            BENOIT, JOSHUA - University Of Cincinnatiitem
                                            BLACKMON, HEATH - University Of Texasitem
                                            BLEDSOE, TIFFANY - University Of Arizonaitem
                                            BOWSHER, JULIA - North Dakota State Universityitem
                                            BUSCH, ANDRE - Max Planck Societyitem
                                            Calla, Bernardaitem
                                            CHAO, HSU - Baylor College Of Medicineitem
                                            Childers, Christopheritem
                                            CLARK, DAVE - University Of Memphisitem
                                            COHEN, LORNA - University Of Illinoisitem
                                            DEMUTH, JEFFERY - University Of Texasitem
                                            DINH, HUYEN - Baylor College Of Medicineitem
                                            DODDAPANENI, HARSHAVARDHAN - Baylor College Of Medicineitem
                                            DOLAN, AMANDA - University Of Rochesteritem
                                            Duan, Jianitem
                                            DUGAN, SHANNON - Baylor College Of Medicineitem
                                            FRIEDRICH, MARKUS - Wayne State Universityitem
                                            GLASTAD, KARL - Georgia Institute Of Technologyitem
                                            GOODISMAN, MICHAEL A. - Georgia Institute Of Technologyitem
                                            HADDAD, STEPHANIE - University Of Memphisitem
                                            HAN, YI - Baylor College Of Medicineitem
                                            HUGHES, DANIEL S. - Baylor College Of Medicineitem
                                            IOANNIDIS, PANAGIOTIS - University Of Genevaitem
                                            VARGAS JENTZSCH, IRIS - University Of Cologneitem
                                            JOHNSTON, J. SPENCER - Texas A&M Universityitem
                                            JONES, JEFFERY - Wayne State Universityitem
                                            KUHN, LESLIE - Michigan State Universityitem
                                            LANCE, DAVID - Animal And Plant Health Inspection Service (APHIS)item
                                            LEE, CHIEN-YUEH - National Taiwan Universityitem
                                            LEE, SANDRA - Baylor College Of Medicineitem
                                            LIN, HAN - National Taiwan Universityitem
                                            LYNCH, JEREMY - University Of Illinoisitem
                                            MOCZEK, ARMIN - Indiana Universityitem
                                            MURALI, SHWETHA - Baylor College Of Medicineitem
                                            MUZNY, DONNA - Baylor College Of Medicineitem
                                            NELSON, DAVID - University Of Tennesseeitem
                                            PALLI, SUBBA - University Of Kentuckyitem
                                            PANFILIO, KRISTEN - University Of Cologneitem
                                            PERS, DAN - University Of Illinoisitem
                                            Poelchau, Monicaitem
                                            QUAN, HONGHU - University Of Illinoisitem
                                            QU, JIAXIN - Baylor College Of Medicineitem
                                            RAY, ANN - Xavier Universityitem
                                            Rinehart, Joeitem
                                            ROBERTSON, HUGH - University Of Illinoisitem
                                            Roehrdanz, Richarditem
                                            ROSENDALE, ANDREW - University Of Cincinnatiitem
                                            SHIN, SEUNGGWAN - University Of Memphisitem
                                            SILVA, CHRISTIAN - University Of Rochesteritem
                                            TORSON, ALEX - North Dakota State Universityitem
                                            WERREN, JOHN - University Of Rochesteritem
                                            WORLEY, KIM - Baylor College Of Medicineitem
                                            Yocum, Georgeitem
                                            ZDOBNOV, EVGENY - University Of Genevaitem
                                            GIBBS, RICHARD - Baylor College Of Medicineitem
                                            RICHARDS, STEPHEN - Baylor College Of Medicine
  Submitted to: Genome Biology
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 10/17/2016
                                    Publication  Date: 11/14/2016
                                    Publication URL: https://handle.nal.usda.gov/10113/5696757Citation: McKenna, D.D., Scully, E.D., Pauchet, Y., Hoover, K., Kirsch, R., Geib, S.M., Mitchell, R.F., Waterhouse, R.M., Ahn, S., Arsala, D., Childers, A.K., Benoit, J.B., Blackmon, H., Bledsoe, T., Bowsher, J., Busch, A., Calla Zalles, B., Chao, H., Childers, C., Clark, D.J., Cohen, L., Demuth, J.P., Dinh, H., Doddapaneni, H., Dolan, A., Duan, J.J., Dugan, S., Friedrich, M., Glastad, K.M., Goodisman, M.D., Haddad, S., Han, Y., Hughes, D.T., Ioannidis, P., Vargas Jentzsch, I.M., Johnston, J., Jones, J.W., Kuhn, L.A., Lance, D.R., Lee, C., Lee, S.L., Lin, H., Lynch, J.A., Moczek, A.P., Murali, S.C., Muzny, D.M., Nelson, D.R., Palli, S.R., Panfilio, K.A., Pers, D., Poelchau, M.F., Quan, H., Qu, J., Ray, A.M., Rinehart, J.P., Robertson, H.M., Roehrdanz, R.L., Rosendale, A.J., Shin, S., Silva, C., Torson, A., Werren, J.H., Worley, K.C., Yocum, G.D., Zdobnov, E.M., Gibbs, R.A., Richards, S. 2016. Genome of the Asian longhorned beetle, Anoplophora glabripennis), a globally significant invasive species, reveals key functional and evolutionary innovations at the beetle-plant interface. Genome Biology. 17:227. doi:10.1186/s13059-016-1088-8.Interpretive Summary: The Asian longhorned beetle (AGLAB) is a globally significant pest that has become locally established in North America. This insect can attack at least 100 different hardwood tree species, including economically important orchard, ornamental and forest tree species and, unlike many other wood-borers, it can colonize both healthy and stressed hosts. Genome sequencing of AGLAB led to the identification of several families of genes capable of degrading plant cell walls and detoxifying plant defensive chemicals. Overall, these features provide AGLAB with the metabolic plasticity needed to feed on a broad range of host trees with different defense chemistry profiles, and contribute to its invasiveness. Functional characterization led to the identification of genes with the abilities to degrade cellulose, xylan, pectin, and xyloglucan, the main components of plant cell walls. Comparative genomic analyses involving 14 additional insect species revealed that many of the genes involved in plant cell wall digestion were originally acquired from bacteria and/or fungi. Many of these genes duplicated several times after their original introduction and diverged functionally, a process that was fundamental to the addition, expansion and enhancement of metabolic pathways in AGLAB. Our results thus begin to establish a genomic basis for the apparent evolutionary success of beetles on plants and allow us to begin to identify genomic factors linked to host range determination and invasiveness, which can provide new tools for risk assessment. Additionally, the identification of genes linked to key digestive and detoxification processes will ultimately provide novel tools for pest management against AGLAB and other invasive pests.
                                Technical Abstract: The Asian longhorned beetle (Anoplophora glabripennis; AGLAB) is a globally significant invasive species capable of inflicting severe feeding damage on many important orchard, ornamental and forest trees. Genome sequencing, annotation, gene expression assays, and functional and comparative genomic studies reveal that the AGLAB genome encodes a uniquely diverse arsenal of enzymes – some encoded by genes originally obtained from bacteria and fungi via horizontal gene transfer (HGT) – that can degrade the main polysaccharide networks in plant cell walls, detoxify plant allelochemicals, and otherwise facilitate specialized feeding on woody plants. AGLAB has the metabolic plasticity needed to feed on plant species with different chemistries, permitting colonization of a diverse range of host plants, and contributing to its highly invasive nature. Its metabolic capacity is further expanded through affiliations with gut microbes. Large expansions of chemosensory genes involved in the reception of pheromones and plant kairomones are consistent with the complexity of chemical cues used by AGLAB to find host plants and mates. Complimentary comparative studies of genes encoding key enzymes involved in the digestion of woody plant tissues or the detoxification of plant allelochemicals were undertaken with the genomes of AGLAB and 14 additional insects, including two additional newly-sequenced beetle genomes (studied here for the first time). These analyses reveal that HGT from bacteria and fungi, followed by gene amplification and functional divergence were fundamental to the addition, expansion and enhancement of the metabolic repertoire of AGLAB and certain other beetles, and to a lesser degree in other phytophagous insects.


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