U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Cereal Disease Lab: St. Paul, MN
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Cereal Rust Bulletins
                    PublicationsSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area St. Paul, Minnesota Cereal Disease Lab Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            PUJOL, VINCENT - Commonwealth Scientific And Industrial Research Organisation (CSIRO)item
                                            FORREST, KERRIE - Department Of Environment And Primary Industriesitem
                                            ZHANG, PENG - University Of Sydneyitem
                                            Rouse, Matthew

                                                         - *Matt*item
                                            HAYDEN, MATTHEW - Department Of Environment And Primary Industriesitem
                                            JONES, DAVID - Australian National Universityitem
                                            TABE, LINDA - Commonwealth Scientific And Industrial Research Organisation (CSIRO)item
                                            LAGUDAH, EVANS - Commonwealth Scientific And Industrial Research Organisation (CSIRO)
  Submitted to: Theoretical and Applied Genetics
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 4/3/2015
                                    Publication  Date: 4/16/2015
                                    Citation: Pujol, V., Forrest, K., Zhang, P., Rouse, M.N., Hayden, M.J., Jones, D., Tabe, L., Lagudah, E. 2015. Mapping of a stem rust resistance locus effective against Ug99 on wheat chromosome 7AL using a RAD-Seq approach. Theoretical and Applied Genetics. 128:1397-1405.Interpretive Summary: Wheat stem rust is a fungal disease of wheat that decreases yield. A strain of the wheat stem rust fungus known as Ug99 emerged in Uganda in 1999 and threatens global wheat production because it is able to infect nearly all wheat varieties. United States spring wheat possesses resistance to stem rust derived from cultivar 'Thatcher'. We identified a stem rust resistance gene in cultivar 'Canthatch' which is related to 'Thatcher'. The resistance gene was identified using new genotyping methods. The identification this gene on wheat chromosome arm 7AL will facilitate the improvement of resistance of United States wheat cultivars to the dangerous Ug99. Ug99 resistant wheat cultivars will protect United States wheat production from yield loss if a Ug99 epidemic were to occur in the United States.
                                Technical Abstract: Wheat stem rust is responsible for major production losses around the world. The development of resistant cultivars is an effective way to manage the disease, but outbreaks can occur when new pathogen races overcome the existing resistances. Ug99 (race TTKSK), and related newly-emerged, highly virulent stem rust races, present a potential threat to global wheat production. The hexaploid wheat cultivar Canthatch has long been known for carrying a suppressor of stem rust resistance on chromosome arm 7DL. Multiple “non-suppressor” mutants of Canthatch were reported to have gained resistance to stem rust races that were recently found to include Ug99 (TTKSK) and related races TTKST and TTTSK. In order to genetically map the suppressor, a mapping population was developed from a cross between the susceptible cultivar Columbus, thought to possess the suppressor, and the resistant Columbus-NS766, a near-isogenic line believed to contain a mutant, non-suppressor allele introgressed from Canthatch. Molecular markers used in mapping were based on two recently developed techniques: i) the 9k iSelect SNP genotyping assay and ii) restriction-site associated DNA sequencing (RAD-Seq) on bulked segregants. These methods led to the rapid identification of markers putatively linked to the stem rust resistance. Surprisingly, genomic sequence information revealed the markers to be located on 7AL, not 7DL, indicating that the phenotype was due to a new resistance locus, rather than the inacti-vated suppressor. We suggest that the 7AL locus is most likely the resistance locus repressed by the 7DL suppressor.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov