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Title:   Author  item
                                            LIM, YEASEUL - US Department Of Agriculture (USDA)item
                                            CHING, SAN - Amlan Internationalitem
                                            JOHNSTON, LEANN - Amlan Internationalitem
                                            CHI, FANG - Amlan Internationalitem
                                            Lillehoj, Hyun
  Submitted to: International Poultry Scientific Forum
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 12/2/2015
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: Enteric disease agents, such as Clostridium perfringens and Escherichia coli, produce detrimental biotoxins that cause significant economic loss annually in the poultry industry.  The objective of this study was to determine the in vitro biotoxin adsorption capability of Varium.  An enzyme-linked immunosorbent assay (ELISA) was developed to evaluate several biotoxins including heat labile toxin (LTB), shiga-like toxin (Shiga), and lipopolysaccharide (LPS) from E. coli; and a-toxin and NetB toxin from C. perfringens.  To do this Varium and the toxins were incubated at several concentrations.  They were then centrifuged to remove the Varium-bound toxin and the supernatant was collected and analyzed to determine the optimum toxin concentration.  Binding percentage was calculated as: 100% x (total concentration - supernatant concentration)/total concentration.  For Varium binding capacity to C. perfringens produced toxins, the binding percentage increased significantly (P < 0.01) from 46 to 96% when the Varium:a-toxin ratio increased from 25:1 to 100:1.  The R-Square of the quadratic response was 0.98 with the equation: Binding % = 34.66 + 0.716 x Ratio - 0.0058 x (Ratio-58.33)2. Varium binding capacity for NetB toxin was 100% as the ratio increased from 25:1 to 100:1.  This indicates that Varium has strong binding capacities for these two toxins that cause necrotic enteritis in poultry.  For E. coli produced toxins, the binding percentage increased from 19 to 83% as the Varium:LPS ratio increased from 50:1 to 400:1.  Varium can bind with LTB from 67 to 100% as the ratio went from 4:1 to 32:1.  Furthermore, Varium bound Shiga from 58 to 100% with ratios from 8:1 to 32:1.  These results indicate that Varium has strong binding capacities with each E. coli toxins, although more so with the exotoxins LTB and Shiga toxins than the endotoxin LPS.  In conclusion, these binding tests indicate that Varium can bind with toxins from C. perfringens and E. coli.


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