U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Animal Biosciences & Biotechnology Laboratory: Beltsville, MD
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Announcements
                        ABBL Journal Club
                    USDA Civil Rights Policy StatementSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Northeast Area Beltsville, Maryland (BARC) Beltsville Agricultural Research Center Animal Biosciences & Biotechnology Laboratory Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            LEE, SUNG - Korean Rural Development Administrationitem
                                            Lillehoj, Hyunitem
                                            JANG, SEUNG - US Department Of Agriculture (USDA)item
                                            LEE, KYUNG WOO - Konkuk Universityitem
                                            KIM, DUK - Konkuk Universityitem
                                            HONG, YEONG - Chung-Ang Universityitem
                                            KIM, JUNG - Rural Development Administration - Koreaitem
                                            CHOE, JEONG-SOOK - Rural Development Administration - Koreaitem
                                            BRAVO, DAVID - Pancosma Sa
  Submitted to: World's Poultry Science Journal
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 5/23/2016
                                    Publication  Date: 6/7/2016
                                    Citation: Lee, S.H., Lillehoj, H.S., Jang, S.I., Lee, K., Kim, D.K., Hong, Y.H., Kim, J.B., Choe, J., Bravo, D.M. 2016. Comparing immune and anti-oxidant effects of selenium sources by in ovo treatment on post-hatch experimental avian necrotic enteritis. World's Poultry Science Journal. 4(7): 420-427.Interpretive Summary: There is increasing efforts to reduce the use of antibiotics in poultry production. Necrotic enteritis (NE) is a major gut illness which affects poultry industry worldwide.  In this study, ARS scientists studies how dietary nutrition such as selenium (Se) can be used to reduce the negative effects of NE. In this study, three different forms of Se were compared with respect to their efficacy on enhancing host immune response in NE-infected young broiler chickens.  The results showed that a certain Se supplementation has a protective effect against NE disease.  Further studies to clarify the role of Se in enhancing protective immunity to NE infection will lead to nutritional modulation of immune response against Clostridium infection.
                                Technical Abstract: This study was conducted to investigate the effects of in ovo injection of selenium (Se) from 3 different sources on modulating the immune and antioxidant responses in broiler chickens in experimental necrotic enteritis (NE). SE contents of Sodium selenite (Na2SeO3 [SS]), Se-enriched yeast (YS), and hydrolyzed soybean protein (B-Taxim [BS]) were 46, 0.2 and 1.1%. respectively.  Broiler eggs were injected at 18 days of embryo age with either 100 μl of PBS alone (CS), SS, YS, or BS in PBS, which contained 20 μg Se source/egg.  PBS-treated, uninfected chickens were designated as the uninfected control group while the remaining chickens (infected CS, SS, YS, or BS) were orally infected with 1.0 × 104 sporulated oocysts of Eimeria maxima at day 14 post-hatch and then 1.0 × 109 colony forming units of Clostridium perfringens at day 18 post-hatch per our established avian NE model.  The infected control CS group showed significantly decreased body weight compared with the uninfected control.  However, the BS group showed significantly increased body weight and significantly lower oocyst output compared with the CS group.  Se-treated groups showed less serum a-toxin and NetB-toxin levels, with BS group showing significantly higher serum antibody levels against these toxins when compared with the CS group.  Finally, serum malondialdehyde (MDA) and two antioxidant-related enzyme activities were significantly increased in the NE-infected CS compared with the uninfected control CS group.  The MDA level was decreased in both SS and BS groups compared with the CS group.  Catalase (CAT) and superoxide dismutase (SOD) activities were decreased in the all Se-treated groups compared with the CS group.  These results suggest that the injection of Se into the amniotic cavity of developing eggs may enhance their immune and antioxidant potential post hatch. BS showed, in general, higher protective effects against NE than other Se sources.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov