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Title:   Author  item
                                            STEUERNAGEL, BURKHARD - John Innes Centeritem
                                            PERIYANNAN, SAMBASIVAM - Commonwealth Scientific And Industrial Research Organisation (CSIRO)item
                                            HERNANDEZ-PINZON, INMACULADA - Sainsbury Laboratoryitem
                                            WITEK, KAMIL - Sainsbury Laboratoryitem
                                            Rouse, Matthew

                                                         - *Matt*item
                                            YU, GUOTAI - John Innes Centeritem
                                            HATTA, ASYRAF - John Innes Centeritem
                                            AYLIFFE, MICK - Commonwealth Scientific And Industrial Research Organisation (CSIRO)item
                                            BARIANA, HARBANS - University Of Sydneyitem
                                            JONES, JONATHAN - Sainsbury Laboratoryitem
                                            LAGUDAH, EVANS - Commonwealth Scientific And Industrial Research Organisation (CSIRO)item
                                            WULFF, BRANDE - John Innes Center
  Submitted to: Nature Biotechnology
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 3/16/2016
                                    Publication  Date: 4/25/2016
                                    Citation: Steuernagel, B., Periyannan, S., Hernandez-Pinzon, I., Witek, K., Rouse, M.N., Yu, G., Hatta, A., Ayliffe, M., Bariana, H., Jones, J., Lagudah, E., Wulff, B. 2016. Rapid cloning of disease-resistance genes in plants using mutagenesis and sequence capture. Nature Biotechnology. 34:652-655. doi:10.1038/nbt.3543.Interpretive Summary: Emerging virulent races of the wheat stem rust pathogen threaten food security. Genetic solutions to protect wheat from stem rust are needed. Wild crop relatives carry disease resistance (R) genes. However, recruiting R genes for crop improvement typically involves long breeding timelines and R genes are often overcome by pathogen adaptation within a few seasons when deployed one at a time. With cloned R genes, we could speed up disease resistance breeding and simultaneously introduce multiple R genes, which should delay resistance-breaking pathogen race evolution and provide more durable resistance. We describe a three-step method (MutRenSeq) for rapid R gene cloning based on (i) chemical mutagenesis and screening for susceptible mutants, (ii) exome capture and sequencing of R gene family members (iii) sequence comparison of wild type and mutants. We use MutRenSeq to clone the important stem rust resistance genes Sr22 and Sr45 from hexaploid bread wheat. Cloning multiple stem rust resistance genes will enable breeding of United States wheat cultivars with durable resistance to stem rust.
                                Technical Abstract: Genetic solutions to protect crops against pests and pathogens are preferable to agrichemicals 1. Wild crop relatives carry immense diversity of disease resistance (R) genes that could enable more sustainable disease control. However, recruiting R genes for crop improvement typically involves long breeding timelines to break linkage to deleterious alleles of other genes (“linkage drag”), and R genes are often overcome within a few seasons when deployed one at a time2. With cloned R genes, we could avoid linkage drag and simultaneously introduce multiple R genes3, which should delay resistance-breaking pathogen race evolution and provide more durable resistance2. We describe a three-step method (MutRenSeq) for rapid R gene cloning based on (i) chemical mutagenesis and screening for susceptible mutants, (ii) exome capture and sequencing of R gene family members (iii) sequence comparison of wild type and mutants. We used MutRenSeq to clone the important stem rust resistance genes Sr22 and Sr45 from hexaploid bread wheat.


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