U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Livestock Issues Research: Lubbock, TX
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    NewsletterSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Plains Area Lubbock, Texas Cropping Systems Research Laboratory Livestock Issues Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Sanchez, Nicoleitem
                                            Carroll, Jeffery

                                                         - *Jeff Carroll*item
                                            SHARON, KATE - Texas Tech Universityitem
                                            ARTHINGTON, JOHN - University Of Floridaitem
                                            LANCASTER, PHILLIP - University Of Florida
  Submitted to: Journal of Animal Science Supplement
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 12/31/2014
                                    Publication  Date: 2/8/2015
                                    Citation: Sanchez, N.C., Carroll, J.A., Sharon, K.P., Arthington, J.D., Lancaster, P.A. 2015. In utero exposure to lipopolysaccharide alters the postnatal metabolic response in heifers.. Journal of Animal Science Supplement. 93(E-Supplement 1):29.Interpretive Summary:
                                Technical Abstract: This study was designed to determine the effect of prenatal lipopolysaccharide (LPS) exposure on the postnatal metabolic response to LPS challenge in heifer calves. Pregnant crossbred cows (n=50) were separated into prenatal stress (PNS; n=25; administered 0.1 microgram per kilogram body weight LPS subcutaneously 233 +/- 15 days of gestation) and saline groups (Cont; n=25).  From these treatments, heifer calves (n= 12 PNS and 11 Cont) were identified at weaning (238 +/- 15 days of age) to subsequently receive an LPS challenge.  On day 0 heifers were fitted with indwelling jugular catheters and were moved into individual pens.  On day 1, heifers were challenged intraveneously with LPS (0.5 microgram per kilogram body weight) at 0 hour (1000h).  Whole blood samples were collected at 30-min intervals from -2 to 8 hours and again at 24 hours relative to the LPS challenge. Serum was isolated and stored at -80C until analyzed for cortisol, glucose, non-esterified fatty acids (NEFA), and blood urea nitrogen (BUN) concentrations. Data were analyzed using the MIXED procedure of SAS specific for repeated measures. There was a treatment x time interaction (P<0.001) for cortisol such that PNS heifers had greater cortisol from 4 to 6.5 hours post-challenge (P<0.001).  There was a treatment x time interaction (P=0.04) for serum glucose such that glucose was greater (P=0.01) in PNS than Cont heifers at 0.5 hours but was greater in Cont than PNS heifers at 2, 4.5, and 7 hours post-challenge.  This resulted in overall time (P<0.001) and treatment (P=0.001) effects such that Cont heifers had greater (68.6 +/- 1.1 miligrams per deciliter) glucose concentrations than PNS heifers (63.4 +/- 1.0 miligrams per deciliter). There was a tendency (P=0.10) for a treatment x time interaction for serum NEFA such that NEFA was greater in Cont than PNS heifers at -2, -1.5, and 7 hours relative to the challenge (P=0.02). Also, there were time (P<0.001) and treatment effects (P=0.002) for NEFA with Cont heifers having greater NEFA (0.30 +/- 0.01 milimolar) than PNS heifers (0.20 +/- 0.01 milimolar). Serum BUN was affected by a treatment x time interaction (P=0.002).  Concentrations of BUN were greater in PNS heifers from -1.5 to -1 hour, 1 to 2 hours, at 4 hours, and from 5 to 24 hours relative to the challenge. Additionally, serum BUN concentrations were affected by time (P<0.001) and treatment (P<0.001) such that PNS heifers had greater BUN (12.1 +/- 0.1 miligrams per deciliter) than Cont heifers (9.5 +/- 0.2 miligrams per deciliter). Results from this study demonstrate that the postnatal metabolic response can be significantly altered with a single exposure to LPS in utero.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov