U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Meat Safety and Quality: Clay Center, NE
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    USMARC Home
                    USMARC Research Units
                        Genetics and Animal Breeding
                                    Livestock Bio Systems
                                    Nutrition, Growth and Physiology
                                    Animal Health GenomicsSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Plains Area Clay Center, Nebraska U.S. Meat Animal Research Center Meat Safety and Quality Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications
- IACUC Summaries

Title:   Author  item
                                            Kemp, Carolineitem
                                            Oliver, Williamitem
                                            Wheeler, Tommyitem
                                            CHISHTI, A - Tufts Universityitem
                                            KOOHMARAIE, MOHAMMAD - Former ARS Employee
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 2/2/2011
                                    Publication  Date: 3/17/2011
                                    Citation: Kemp, C.M., Oliver, W.T., Wheeler, T.L., Chishti, A.H., Koohmaraie, M. 2011. Growth and development of skeletal muscle in mu-calpain knockout mice [abstract]. FASEB Journal. 25:707.6.Interpretive Summary: Not required.
                                Technical Abstract: The calpain system has been identified as a potential candidate in muscle growth and development due to its role in a variety of cellular processes such as cytoskeletal remodeling and myogenesis.  The objective of this study was to evaluate growth and development of skeletal muscle in mu-calpain knockout mice (KO) compared to wild type C57/BL6 mice (WT).  At 3, 5, 10, 20, and 30 weeks of age, WT and KO mice (n=6) were sacrificed and extensor digitorum longus (EDL), tibialis anterior (TA), quadriceps, gastrocnemius, and soleus muscles were quantitatively dissected.  Body composition was evaluated by proximal analysis on WT and KO mice (n = 6) for each age group.  Muscle weights and total RNA, DNA, and protein were unaffected by genotype.  At 30 weeks, there was an increased potential for protein synthesis and muscle hypertrophy in the KO mice as indicated by the increased protein:DNA (P = 0.0349) and RNA:DNA (P = 0.016).  This is reflected in the body composition analysis, which showed KO mice had increased protein content at the expense of lipid content at 30 weeks (P = 0.0102).  These data suggest the calpain system is involved in muscle growth and appears to be involved in muscle wasting at 30 weeks of age.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov