U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Mycotoxin Prevention and Applied Microbiology Research: Peoria, IL
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    NCAUR homepageSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area Peoria, Illinois National Center for Agricultural Utilization Research Mycotoxin Prevention and Applied Microbiology Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications
- MPM Home Page
- Mission Statement
- Strategic Goals
- Major Accomplishments
- Visiting Scientists

Title:   Author  item
                                            Appell, Michaelitem
                                            Jackson, Michael

                                                         - *Mike*item
                                            Wang, Lijuanitem
                                            BOSMA, WAYNE - Bradley University
  Submitted to: Journal of Liquid Chromatography and Related Technologies
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 10/25/2015
                                    Publication  Date: 12/7/2015
                                    Publication URL: https://handle.nal.usda.gov/10113/5695388Citation: Appell, M., Jackson, M.A., Wang, L.C., Bosma, W.B. 2015. Determination of citrinin using molecularly imprinted solid phase extraction purification, HPLC separation, and fluorescence detection. Journal of Liquid Chromatography and Related Technologies. 38(20):1815-1819.Interpretive Summary: A new synthetic material was developed to enable better detection methods for the toxin citrinin in corn. Citrinin is a toxin produced by fungi that occasionally contaminate agricultural commodities. Contamination reduces commodity value and poses health risks to consumers and animals. Computational methods and synthetic receptor technology aided in the development of a material to help monitor and prevent exposure to the adverse effects of this toxin. The material was successfully applied to clean-up corn samples and enable accurate determination of citrinin using modern analytical instrumentation with fluorescence detection. These research findings will be of interest to food safety scientists, analytical scientists, and chemists. The detection method provides the food industrywith a new approach to monitor the toxin citrinin in corn.
                                Technical Abstract: A liquid chromatography based method to detect citrinin in corn was developed using molecularly imprinted solid phase extraction (MISPE) sample clean-up. Molecularly imprinted polymers were synthesized using 1,4-dihydroxy-2-naphthoic acid as the template and an amine functional monomer. Density functional calculations suggest the mimic template interacts with the functional monomer in a similar manner as citrinin. Freundlich isotherm analysis indicated the template provided a significant imprinting effect for citrinin binding. A high performance liquid chromatography with fluorescence detection (HPLC-FD) method to detect citrinin in maize was developed utilizing the imprinted polymers for sample clean-up (excitation at 330 nm; emission at 500 nm). Recoveries of citrinin in spiked corn samples were between 82.3-91.5%. This study demonstrates that molecularly imprinted polymers are applicable in the solid phase extraction clean-up of corn samples for citrinin determination by HPLC-FD.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov