U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Animal Genomics and Improvement Laboratory: Beltsville, MD
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                                    Software
                People

                    Staff Listing
                                    Careers


        


                    AIP
                        Home
                                    About
                                    Publications
                                    Software
                                    People
                                    Collaborations
                                    Careers
                                    Centennial
                                    Contact
                                    Dairy industry links
                    DirectionsSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Northeast Area Beltsville, Maryland (BARC) Beltsville Agricultural Research Center Animal Genomics and Improvement Laboratory Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications
- IACUC
- Home
- About
- Publications
- Software
- People
- Collaborations
- Careers
- Centennial
- Contact
- Dairy industry links
- Home
- Queries login
- News
- Animal Evaluation lists
- Genotype counts

Title:   Author  item
                                            ZHAO, CHUNPING - Northwest Agriculture And Forestry Universityitem
                                            ZAN, LINSEN - Northwest Agriculture And Forestry Universityitem
                                            WANG, YAN - University Of Marylanditem
                                            UPDIKE, M. SCOTT - University Of Marylanditem
                                            Liu, Ge

                                                         - *George*item
                                            BEQUETTE, BRIAN - University Of Marylanditem
                                            Baldwin, Ransom

                                                         - *Randy*item
                                            SONG, JIUZHOU - University Of Maryland
  Submitted to: Livestock Science
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 11/30/2013
                                    Publication  Date: 12/16/2013
                                    Citation: Zhao, C., Zan, L., Wang, Y., Updike, M., Liu, G., Bequette, B.J., Baldwin, R.L., Song, J. 2013. Functional proteomic and interactome analysis of proteins associated with beef tenderness in angus cattle. Livestock Science. 161:201-209.Interpretive Summary: Beef is a source of high-quality protein for the human population, and beef tenderness has a significant influence on beef palatability, consumer expectation, and industry profitability. To further characterize factors affecting beef tenderness, functional proteomics and bioinformatics interactome analysis were performed on longissimus dorsi (LD) muscle of Angus cattle. Eight proteins were identified from this study which had meaningful interactions with the level of tenderness of the beef.  These proteins are involved with carbohydrate metabolism, as well as protein breakdown and response to stress. Because tenderness is such an important part of beef sales, the results of this study provide important information for the development of new methods to ensure beef tenderness in production systems.
                                Technical Abstract: Beef is a source of high quality protein for the human population, and beef tenderness has significant influence on beef palatability, consumer expectation and industry profitability. To further elucidate the factors affecting beef tenderness, functional proteomics and bioinformatics interactome analysis were performed on longissimus dorsi (LD) tissue from Angus cattle.  Proteins were analyzed by SDS-PAGE and linearly regressed against Warner-Bratzler shear forces (WBSF). Seventeen electrophoretic bands were revealed to be correlated with beef tenderness.  Two of the bands with the most significant correlations were selected and  submitted to LCMS/MS for analysis. Eight proteins, beta-enolase (ENOB), L-lactate dehydrogenase A chain (LDHA), glyceraldehyde-3-phosphate dehydrogenase (G3P), carbonic anhydrase 3 (CAH3), phosphoglycerate mutase 2 (PGAM2), peroxiredoxin-6 (PRDX6), myosin heavy chain 1 (MYH1) and myosin heavy chain 7 (MYH7), were identified from these two bands. Interactions of these 8 protein markers were constructed using Cytoscape and further functional annotation analysis showed these proteins of the interactome were enriched in metabolic pathways, such as carbohydrate metabolic and catabolic processes, apoptosis, peptidase activity and response to stress. The results of this study provide a novel insight into the interaction of proteins functionally correlated with beef tenderness.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov