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Title:   Author  item
                                            CIASCA, B. - National Research Council - Italyitem
                                            LATTANZIO, V. M. - National Research Council - Italyitem
                                            TERZI, V. - Agricultural Research Council (CRA)item
                                            GHIZZONI, R. - Agricultural Research Council (CRA)item
                                            McCormick, Susanitem
                                            PASCALE, M. - National Research Council - Italy
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 5/14/2015
                                    Publication  Date: 5/14/2015
                                    Citation: Ciasca, B., Lattanzio, V.T., Terzi, V., Ghizzoni, R., McCormick, S.P., Pascale, M. 2015. Study of natural occurrence of T-2 and HT-2 toxins and their glucosyl derivatives from field barley to malt by high resolution orbitrap mass spectrometry [abstract].Interpretive Summary:
                                Technical Abstract: A recent European Union Commission Recommendation (2013/165/EU), asked for collection of more data on the occurrence of T-2 and HT-2 toxins in cereals and cereal products and emphasized that if the method of analysis enables it, it would be appropriate to collect data of the occurrence of masked mycotoxins of T-2 and HT-2 toxin”. Addressing these issues, the aim of this work was to obtain more comprehensive information on the co-occurrence of T-2 and HT-2 toxins with their mono-glucosylated derivatives in naturally contaminated cereal samples. For these purposes barley samples originating from a Northern Italian area, were analyzed by LC-HRMS for the presence of T-2, HT-2, and related glucosyl derivatives. Quantitative analysis of T-2 and HT-2 glucosides was performed using a recently made available standard of T2 glucoside. A widespread co-occurrence of the glucosyl derivative of HT-2 with the unconjugated toxin was observed in unprocessed barley grains, whereas the monoglucosyl derivative of T2 toxin was detected only in few samples and low μg/kg levels. The ratio between glucosylated toxins (sum of T-2 and HT-2 toxin glucosides) and native toxins (sum of T-2 and HT-2 toxins) ranged from 2 to 283%. Moreover, using retrospective analysis of full scan HRMS chromatograms, samples were also screened for the presence of other type-A trichothecenes, namely neosolaniol, diacetoxyscirpenol and their mono-glucosyl derivatives, which were detected at trace levels. A subset of nine different samples was subjected to micro-maltation in order to carry out a preliminary investigation on the co-occurrence of T-2, HT-2 toxins and their glucosides along the malting process. Mycotoxin reduction from cleaned barley to malt was observed at rates ranging from 4% to 87%.


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