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ARS Home Northeast Area Geneva, New York Grape Genetics Research Unit (GGRU) Research Publications at this Location





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Title:   Author  item
                                            YANG, SHANSHAN - Cornell Universityitem
                                            FRESNEDO, JONATHAN - Cornell Universityitem
                                            TAKACS, ELIZABETH - Cornell Universityitem
                                            BARBA, PAOLA - Cornell Universityitem
                                            HYMA, KATIE - Cornell Universityitem
                                            Lillis, Jacquelynitem
                                            ACHARYA, CHARLOTTE - Cornell Universityitem
                                            FISHER, ANN - Cornell Universityitem
                                            COTE, LINDA - Cornell Universityitem
                                            MANNS, DAVID - Cornell Universityitem
                                            RYONA, IMELDA - Cornell Universityitem
                                            GADOURY, DAVID - Cornell Universityitem
                                            SEEM, BOB - Cornell Universityitem
                                            SACKS, GAVIN - Cornell Universityitem
                                            MANSFIELD, ANNA KATHERINE - Cornell Universityitem
                                            Ledbetter, Craigitem
                                            LUBY, JIM - University Of Minnesotaitem
                                            HEMSTAD, PETER - University Of Minnesotaitem
                                            FENNELL, ANNE - South Dakota State Universityitem
                                            HWANG, CHIN-FENG - Missouri State Universityitem
                                            WALKER, ANDY - University Of Californiaitem
                                            RIAZ, SUMMAIRA - University Of Californiaitem
                                            COUSINS, PETER - E & J Gallo Wineryitem
                                            Londo, Jasonitem
                                            Buckler, Edward

                                                         - *Ed*item
                                            MITCHELL, SHARON - Cornell Universityitem
                                            SCHWEITZER, PETER - Cornell Universityitem
                                            SUN, QI - Cornell Universityitem
                                            Cadle-Davidson, Lanceitem
                                            REISCH, BRUCE - Cornell University
  Submitted to: Annual International Plant & Animal Genome Conference
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 11/16/2014
                                    Publication  Date: 1/1/2015
                                    Publication URL: https://pag.confex.com/pag/xxiii/webprogram/Paper15597.htmlCitation: Yang, S., Fresnedo, J., Takacs, E., Barba, P., Hyma, K., Lillis, J.A., Acharya, C., Fisher, A., Cote, L., Manns, D., Ryona, I., Gadoury, D., Seem, B., Sacks, G., Mansfield, A., Ledbetter, C.A., Luby, J., Hemstad, P., Fennell, A., Hwang, C., Walker, A., Riaz, S., Cousins, P., Londo, J.P., Buckler Iv, E.S., Mitchell, S., Schweitzer, P., Sun, Q., Cadle Davidson, L.E., Reisch, B. 2015. VitisGen on the road: mapping the way to the next generation of grapes. Annual International Plant & Animal Genome Conference. P0873: 15597.Interpretive Summary:
                                Technical Abstract: The USDA-NIFA VitisGen project (Award No. 2011-51181-30635) aims to optimize grape cultivar improvement through the integration of grape breeding, cutting edge genomics technology, precision phenotyping, socioeconomic research, and extension. VitisGen is a five-year project involving 25 co-PIs at 11 institutions, focused on identifying the genes associated with economically important traits and on developing tools for marker-assisted selection (MAS). To date, 23,328 vines were genotyped for marker development or application. Seventeen F1 families were prioritized as core sets to develop new markers. Genotyping-by-sequencing (GBS) technology was applied to obtain single nucleotide polymorphism (SNP) markers, which were used for linkage map construction and subsequent quantitative trait locus (QTL) mapping. Three trait evaluation centers provided quantitative phenotypic data for analysis of powdery mildew resistance, low temperature response, and fruit quality. The average number of raw SNP markers per population was 150,000, of which 10% with high quality were chosen on average for linkage mapping. Twenty-two parental maps were generated from eleven crosses and utilized for QTL mapping. At least one major QTL was identified for each trait. Further analyses such as candidate gene scans and PCR marker development are in process. In the meantime, the project developed a high-throughput protocol for SSR processing and analysis, which allowed breeders to utilize MAS to increase the efficiency of seedling trials. The results so far support the VitisGen vision to pursue a better understanding of grape genomics and to implement molecular procedures to accelerate the breeding process, which will ultimately benefit researchers, breeders, growers, and consumers.


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