U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Horticultural Crops Research Unit: Corvallis, OR
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Other
                        History
                                    Useful Links
                    HortHappenings News PageSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Pacific West Area Corvallis, Oregon Horticultural Crops Research Unit Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Bushakra, Jillitem
                                            Bassil, Nahlaitem
                                            BRYAN, D - Danforth Plant Science Centeritem
                                            MOCKLER, T - Danforth Plant Science Centeritem
                                            DOSSETT, M - Agriculture And Agri-Food Canadaitem
                                            Gilmore, Barbaraitem
                                            Peterson, Maryitem
                                            BRADISH, C - North Carolina State Universityitem
                                            FERNANDEX, G - North Carolina State Universityitem
                                            Lee, Jungminitem
                                            Finn, Chad
  Submitted to: Plant and Animal Genome Conference
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 5/20/2015
                                    Publication  Date: 5/20/2015
                                    Citation: Bushakra, J., Bassil, N.V., Bryan, D., Mockler, T., Dossett, M., Gilmore, B.S., Peterson, M.E., Bradish, C., Fernandex, G., Lee, J., Finn, C.E. 2015. Black raspberry genomic and genetic resource development to enable cultivar improvement[abstract]. Plant and Animal Genome XXIII Conference.Interpretive Summary:
                                Technical Abstract: This project incorporates use of phenotypic, genotypic and genomic data to advance and streamline identification of traits of economic interest and to develop molecular markers for marker assisted breeding of black raspberry (Rubus occidentalis L.). A lack of adapted, disease resistant cultivars has led to the steady decline of the black raspberry (BR) industry in the US. Interest in production and breeding new cultivars has been fueled by news regarding the potential health benefits of black raspberry bioactive compounds. To accomplish our goals, we are developing genomic tools including microsatellite and single nucleotide polymorphism (SNP) markers for construction of linkage and physical maps, and a draft genome assembly. Genotyping by sequencing (GBS) through a custom data pipeline identified over 35,000 SNP loci in population ORUS 4305 parents and 105 progeny. Up to 7,900 loci appear suitable for linkage mapping and have less than 10% missing data. The current genome assembly consists of 2,220 scaffolds. Two related full-sibling populations were replicated and planted at five production sites (OR, WA, OH, NC, and NY). We are taking detailed trait measurements including primocane vigor, flowering and fruiting, disease and aphid tolerance, and plant architecture to assess the influence of environment on genotype (GxE). Initial analysis of GxE on primocane vigor indicated that individual genotypes showed significant variation among sites. A densely populated genetic linkage map will be used to improve the draft genome assembly, for quantitative trait locus (QTL) mapping, and comparative genomic studies with other Rosaceae species.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov