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Title:   Author  item
                                            HILDEBRAND, AMANDA - University Of Californiaitem
                                            SZEWCZYK, EDYTA - University Of Californiaitem
                                            LIN, HUI - University Of Californiaitem
                                            Kasuga, Takaoitem
                                            FAN, ZHILIANG - University Of California
  Submitted to: Applied and Environmental Microbiology
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 11/7/2014
                                    Publication  Date: 1/15/2015
                                    Publication URL: http://aem.asm.org/content/81/2/597.full.pdf+html?sid=6333baa2-48ad-4db4-b5d6-cb2a45eb0Citation: Hildebrand, A., Szewczyk, E., Lin, H., Kasuga, T., Fan, Z. 2015. Engineering Neurospora crassa for improved cellobiose and cellobionate production. Applied and Environmental Microbiology. 81:597-603.Interpretive Summary:
                                Technical Abstract: We report engineering Neurospora crassa to improve the yield of cellobiose and cellobionate from cellulose. A previously engineered strain of N. crassa (F5) with six out of seven bgl genes
knocked out was shown to produce cellobiose and cellobionate directly from cellulose without the addition of exogenous cellulases. In this study, the F5 strain was further modified to improve the yield of cellobiose and cellobionate from cellulose by increasing cellulase production and decreasing product consumption. The effects of two catabolite repression genes cre-1 and ace-1 on cellulase production were investigated. The F5'ace-1 mutant showed no improvement over the wild type. The F5'cre-1 and F5'ace-1'cre-1 strains showed improved cellobiose dehydrogenase and exoglucanase expression. However, this improvement in cellulase expression did not lead to an improvement in cellobiose or cellobionate production. The cellobionate phosphorylase gene (ndvB) was deleted from the genome of F5'ace-1'cre-1 to prevent theconsumption of cellobiose and cellobionate. Despite a slightly reduced hydrolysis rate, the F5'ace-1'cre-1'ndvB strain converted 79% of the cellulose consumed to the desired products,
cellobiose and cellobionate, as compared to 19% by the strain F5'ace-1'cre-1.


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