U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Tropical Crop and Commodity Protection Research: Hilo, HI
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Scientist Pages
                        Acebes-Doria, Angelita
                                    Cha, Dong
                                    Follett, Peter
                                    Johnson, Melissa
                                    Liang, Peishih
                                    Manoukis, Nicholas
                                    Siderhurst, Matthew
                                    Sun, XiuxiuSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Pacific West Area Hilo, Hawaii Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center Tropical Crop and Commodity Protection Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            FRANCISCHETTI, IVO - National Instiute Of Allergy And Infectious Diseases (NIAID, NIH)item
                                            ANDERSON, JENNIFER - National Instiute Of Allergy And Infectious Diseases (NIAID, NIH)item
                                            Manoukis, Nicholasitem
                                            PHAM, VAN - National Instiute Of Allergy And Infectious Diseases (NIAID, NIH)item
                                            RIBEIRO, JOSE - National Instiute Of Allergy And Infectious Diseases (NIAID, NIH)
  Submitted to: Journal of Proteomics
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 7/1/2011
                                    Publication  Date: 11/18/2011
                                    Citation: Francischetti, I.M., Anderson, J.M., Manoukis, N., Pham, V.M., Ribeiro, J.M. 2011. An insight into the sialotranscriptome and proteome of the coarse bontlegged tick, Hyalomma marginatum rufipes. Journal of Proteomics. 2892-2908.Interpretive Summary: Ticks are mites specialized in acquiring blood from vertebrates as their sole source of food and are important disease vectors to humans and animals. Among the specializations required for this peculiar diet, ticks evolved a sophisticated salivary potion that can disarm their host's hemostasis, inflammation, and immune reactions. Previous transcriptome analysis of tick salivary proteins has revealed many new protein families indicative of fast evolution, possibly due to host immune pressure. The hard ticks (family Ixodidae) are further divided into two basal groups, of hich the Metastriata have 11 genera. While salivary transcriptomes have been described for some of these genera, no tick of the genes Hyalomma has been studied so far.
                                Technical Abstract: The analysis of 2,084 expressed sequemce tags (EST) from a salivary gland cDNA library lead to the assembly of 1,167 clusters of sequences, allowing identification of 255 contigs and singletons associated with putative salivary secreted componenets, from which 56 coding sequences (CDS) were extracted, annotated, and deposited into GenBank. Additionally, 41 CDS associated with housekeeping functions were also extracted. The data are organized into hyperlinked spreadsheets that can be obtained from the Additional files 1 and 2 available at http://exon.niaid.nih.gov/transcriptome/Hmrufipes/Hmr-S1.xls and http://exon.niaid.nih.gov/trancriptome/Hmrufipes/Hmr-S2.xls, respectively. Several protein families previously found in tick salivary transcriptomes were identified such as the Kunitz, basil tail, madanin, lipocalin, glycine-rich, mucins, immunity-related, and 8.9-kDa family, as well as protein families previously found only in Dermacentor ticks, such as the 9-kDa family. Most of these proteins have no known function. Many orphan proteins were found that have been no match to known proteins but have signal peptides indicative of secterion, suggesting these to be Hyalomma-specific proteins. This annotated data set can assist the discovery of new targets for anti-tick vaccines as well as help to identify pharmacologically active proteins.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov