U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Meat Safety and Quality: Clay Center, NE
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    USMARC Home
                    USMARC Research Units
                        Genetics and Animal Breeding
                                    Livestock Bio Systems
                                    Nutrition, Growth and Physiology
                                    Animal Health GenomicsSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Plains Area Clay Center, Nebraska U.S. Meat Animal Research Center Meat Safety and Quality Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications
- IACUC Summaries

Title:   Author  item
                                            Berry, Elaineitem
                                            Wells, James

                                                         - *Jim*item
                                            Durso, Lisaitem
                                            Bono, James

                                                         - *Jim*item
                                            Friesen, Kristinaitem
                                            Woodbury, Bryanitem
                                            SUSLOW, TREVOR - University Of Californiaitem
                                            LOPEZ-VELASCO, GABRIELA - University Of Californiaitem
                                            Millner, Patricia
  Submitted to: International Association for Food Protection Proceedings
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 3/5/2013
                                    Publication  Date: 7/28/2013
                                    Citation: Berry, E.D., Wells, J., Durso, L.M., Bono, J.L., Friesen, K.M., Woodbury, B.L., Suslow, T.V., Lopez-Velasco, G., Millner, P.D. 2013. Effect of proximity to a cattle feedlot on the occurrence of Escherichia coli 0157:H7-positive pest flies in a leafy green crop. International Association for Food Protection Proceedings. [abstract]P3-132 Page No 65.Interpretive Summary:
                                Technical Abstract: Introduction: Escherichia coli O157:H7 outbreaks linked to produce have focused attention on cattle as contamination sources.  Cattle pest flies can harbor this pathogen, and may disseminate it to produce.  The Leafy Greens Marketing Agreement proposes an interim guidance distance of 400 feet between concentrated animal feeding operations and leafy green crops to reduce contamination risk.  

Purpose: The objective was to determine the occurrence of E. coli O157:H7-positive pest flies in leafy greens planted at different distances from a cattle feedlot.

Methods: Spinach was planted to nine plots sited at 200, 400, and 600 feet from a 6,000-head feedlot (3 plots each distance).  Sticky traps were used to capture flies at the spinach plots and the feedlot in June through September.  Traps were removed to the lab after 48 h, and flies were identified, counted, and pooled by species (up to ten flies per pool). Presence of E. coli O157:H7 was determined by immunomagnetic separation and plating in up to ten fly pools per site.  

Results: Prevalence of E. coli O157:H7 in house flies (Musca domestica) and face flies (Musca autumnalis) was 17.1 and 15.8%, respectively, and did not differ (P > 0.05).  Prevalence in house flies tended to be higher (P = 0.09) than in flesh flies (Sarcophagidae; 10.7%) and was higher (P < 0.05) than in blow flies (Calliphoridae; 7.6%) and stable flies (Stomoxys calcitrans; 0.63%).  Overall, the percentage of O157:H7-positive fly pools was highest (P < 0.05) at the edge of the feedlot (18.5%), although the pathogen was found in 10.4, 8.5, and 9.5% of fly pools at 200, 400, and 600 feet from the feedlot.

Significance: Current leafy green field distance guidelines may not be adequate to limit the occurrence of pest flies in crops planted near cattle feedlots.  Further work is needed to clarify the risk for transmission of E. coli O157:H7 to leafy greens by flies.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov