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ARS Home Midwest Area Madison, Wisconsin U.S. Dairy Forage Research Center Cell Wall Biology and Utilization Research Research Publications at this Location





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Title:   Author  item
                                            Zeller, Wayneitem
                                            Sullivan, Michaelitem
                                            MUELLER-HARVEY, IRENE - University Of Readingitem
                                            Grabber, Johnitem
                                            RAMSAY, AINA - University Of Readingitem
                                            DRAKE, CHRIS - University Of Readingitem
                                            BROWN, RONALD - University Of Reading
  Submitted to: American Chemical Society Abstracts
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 12/19/2014
                                    Publication  Date: 3/23/2015
                                    Citation: Zeller, W.E., Sullivan, M.L., Mueller-Harvey, I., Grabber, J.H., Ramsay, A., Drake, C., Brown, R.H. 2015. Impact by condensed tannins with different mean degrees of polymerization on protein precipitation [abstract]. 249th American Chemical Society Meeting, March 22-26, 2015, Denver, Colorado. Abstract No. AGFD 51.Interpretive Summary:
                                Technical Abstract: Condensed tannins (CTs) isolated from white clover (Trifolium repens) flowers and big trefoil (Lotus pedunculatus) leaves were evaluated in precipitation studies with bovine serum albumin (BSA), lysozyme (LYS), and alfalfa leaf protein (ALF). The CTs were of similar compositions, but differed in their mean degree of polymerization (mDP); medium CTs with mDP ~9 and large CTs with mDP ~18. At pH 6.5, large CTs exhibited similar protein precipitation behaviors and were significantly more effective precipitants than medium CTs. All CTs precipitated ALF more effectively than BSA or LYS. Medium CTs exhibited similar capacities to precipitate ALF, but showed small but significant differences in their capacity to precipitate BSA and LYS. Aggregation of CT-protein complexes may have contributed to ALF and BSA, but not LYS precipitation. This study, one of the first to use CTs of confirmed high purity, demonstrated that the mDP of CTs influences protein precipitation efficacy.


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