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Title:   Author  item
                                            Zalapa, Juanitem
                                            BOUGIE, T - University Of Wisconsinitem
                                            BOUGIE, T - University Of Wisconsinitem
                                            SCHLAUTMAN, B - University Of Wisconsinitem
                                            Wiesman, Ericitem
                                            GUZMAN, A - University Of Wisconsinitem
                                            FAJARDO, D - University Of Wisconsinitem
                                            Steffan, Shawnitem
                                            SMITH, T - Agriculture And Agri-Food Canada
  Submitted to: Annals of Applied Biology
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 9/15/2014
                                    Publication  Date: 11/6/2014
                                    Citation: Zalapa, J.E., Bougie, T.C., Bougie, T.A., Schlautman, B.J., Wiesman, E., Guzman, A., Fajardo, D.A., Steffan, S., Smith, T. 2014. Clonal diversity and genetic differentiation revealed by SSR markers in wild Vaccinium macrocarpon and Vaccinium oxycoccos. Annals of Applied Biology. 166(2):196-207.Interpretive Summary: This study genetically characterized natural populations of American cranberry (Vaccinium macrocarpon) and small cranberry (V. oxycoccos) using DNA markers. The data collected from this study offers insight into natural cranberry diversity and differentiation among two closely related species. This data will be useful for future studies about natural cranberry diversity and natural population characteristics as well as other cranberry breeding and genetics studies. Unique cranberry types can be generated by the hybridization of V. macrocarpon and V. oxycoccos populations. Therefore, a genetic fingerprint allowing the differentiation each species will aid further studies of genetic diversity and pedigree analysis of natural and breeding populations.
                                Technical Abstract: American cranberry (Vaccinium macrocarpon Ait.) is a perennial, woody plant species, native to North American bogs and wetlands. Cranberries represent one of the few agriculturally important native plants in which wild gene pools are still readily available within the undeveloped wetlands the northern U.S. and Canada. Past studies have reported low genetic variation in V. macrocarpon at the species and population level. However, in this study, we characterized 229 samples of wild V. macrocarpon and V. oxycoccos (small cranberry) and 22 control accessions using microsatellite markers and observed substantial genetic variation and differentiation within and among populations and species. While V. macrocarpon was analyzed using 108 alleles from eleven microsatellite loci revealing 42 unique genotypes, V. oxycoccos was analyzed using 156 alleles from eight loci revealing 28 unique genotypes. There were a total of 182 alleles found in both species combined with 156 of those alleles present in V. oxycoccos and 84 alleles found in V. macrocarpon. All eight loci possessed species-specific alleles with V. oxycoccos possessing 98 private alleles versus 26 private alleles found V. macrocarpon, and 58 alleles were found in common between both species. Our data will be valuable not only for future wild cranberry diversity and population genetics research, but for other cranberry breeding and genetics studies.


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