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Title:   Author  item
                                            Nicholson, Tracyitem
                                            Shore, Sarahitem
                                            Register, Karenitem
                                            Bayles, Darrellitem
                                            KINGSLEY, ROBERT - Wellcome Trust Sanger Instituteitem
                                            Brunelle, Brian
  Submitted to: Veterinary Microbiology
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 10/27/2015
                                    Publication  Date: 1/15/2016
                                    Citation: Nicholson, T.L., Shore, S., Register, K.B., Bayles, D.O., Kingsley, R.A., Brunelle, B.W. 2016. Comparative genomic analysis of the swine pathogen Bordetella bronchiseptica strain KM22. Veterinary Microbiology. 182:87-94.Interpretive Summary: Bordetella bronchiseptica swine isolate KM22 has been used in experimental infections of swine as a model of clinical B. bronchiseptica infections within swine herds and to study host-to-host transmission. In this report we use the genome sequence of KM22 for the purpose of performing comparative genomics analyses to identify genomic sequences unique to KM22 and to compare KM22 to the other six previously sequenced B. bronchiseptica strains. This information can be directly used to design improved vaccines and therapeutic interventions to eradicate B. bronchiseptica from swine herds that do not rely on antimicrobial use.
                                Technical Abstract: B. bronchiseptica is pervasive in swine and plays multiple roles in respiratory disease as well as enhancing colonization by other bacterial pathogens and increasing the severity of disease associated with both viral and bacterial pathogens. The goal of this study was to use the genome sequence of KM22 for the purpose of performing comparative genomics analyses to identify genomic sequences unique to KM22 and to compare KM22 to the other six previously sequenced B. bronchiseptica strains. Overall, the KM22 genome sequence is more similar to the genome sequences of the strains isolated from animals (RB50, 1289, and 253) than the strains isolated from humans (D445, Bbr77, and MO149). The most highly conserved Bvg-regulated genes present in all seven strains were bopN, fimB, bvgA, and fimC. In contrast, the Bvg-regulated genes present in all seven strains with the highest sequence divergence were fimN, fim2, fhaL, and fhaS. A total of eight major fimbrial subunit genes (fimA, fim2, fim3, fimN, fimX, fim2-like KM22_03370, KM22_03368, and KM22_03128) were identified in KM22. The fimNX locus is the chromosomal region containing fimbrial genes where the greatest sequence divergence was observed among the B. bronchiseptica strains analyzed. With the exception of KM22_03128, quantitative real-time PCR data demonstrated that all of the major fimbrial subunit genes identified in KM22 are expressed and regulated by BvgAS.


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