U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Cell Wall Biology and Utilization Research: Madison, WI
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area Madison, Wisconsin U.S. Dairy Forage Research Center Cell Wall Biology and Utilization Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Zeller, Wayneitem
                                            MUELLER-HARVEY, IRENE - University Of Readingitem
                                            DRAKE, CHRIS - University Of Readingitem
                                            Grabber, John
  Submitted to: American Chemical Society Abstracts
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 4/20/2014
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: Unambiguous investigation of condensed tannin (CT) structure-activity relationships in biological systems requires the use of highly enriched CT fractions of defined chemical purity. Purification of CTs from Sorghum bicolor, Trifolium repens, Theobroma cacao, Lespedeza cuneata, Lotus pedunculatus, and Lotus corniculatus was accomplished by Sephadex LH-20 chromatography using gradient elution schemes. We found that the degree of CT enrichment and need for further purification by chromatography could be rapidly assessed in minutes by subjecting fractions to 1H-NMR spectroscopy. These assessments were independently corroborated by a more time-consuming, but quantitative thiolysis procedure. Further examination of CT fractions by 1H-13C HSQC NMR spectroscopy provided a more detailed assessment of impurities, some of which (carbohydrate- and lipid-derived) avoid detection by UV absorption-based detectors commonly used for CT chromatography. Researchers can now rely on a rapid 1H-NMR analysis to assess the purity of CT fractions, even with low-field (360 MHz) NMR instruments.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov