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Title:   Author  item
                                            XIN, LI - University Of Minnesotaitem
                                            SANGHYUN, SHIN - University Of Minnesotaitem
                                            HEINEN, SHANE - University Of Minnesotaitem
                                            DILL-MACKY, RUTH - University Of Minnesotaitem
                                            BERTHILLER, FRANZ - University Of Natural Resources & Applied Life Sciences - Austriaitem
                                            CLEMENTE, THOMAS - University Of Nebraskaitem
                                            McCormick, Susanitem
                                            MUEHLBAUER, GARY - University Of Minnesota
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 12/5/2013
                                    Publication  Date: 12/5/2013
                                    Citation: Xin, L., Sanghyun, S., Heinen, S., Dill-Macky, R., Berthiller, F., Clemente, T., McCormick, S.P., Muehlbauer, G. 2013. Transgenic wheat carrying a barley UDP-glucosyltransferase exhibit high levels of Fusarium head blight resistance by detoxifying trichothecenes [abstract]. U.S. Wheat Barley Scab Forum.Interpretive Summary:
                                Technical Abstract: Fusarium head blight (FHB) is a worldwide disease of wheat and barley, mainly caused by Fusarium graminearum. During infection, the fungal pathogen produces trichothecene mycotoxins, such as deoxynivalenol (DON) and nivalenol (NIV) that increase fungal virulence. Moreover, grains contaminated with trichothecenes threaten the health of humans and animals that consume them. Previous work had identified a barley UDP-glucosyltransferase (HvUGT13248) gene that exhibited resistance to DON via the conversion to DON-3-O-glucoside (D3G) in transgenic yeast and Arabidopsis. We developed transgenic wheat lines constitutively overexpressing the HvUGT13248 gene in the background of cultivar Bobwhite and CB037. We performed point-inoculation tests in the greenhouse for three seasons (2011 spring, 2011 fall and 2012 spring) and found that transgenic wheat exhibited significantly higher type II resistance compared with the untransformed parental lines. Moreover, in two field tests (2012 and 2013 summer), HvUGT13248-overexpressing wheat lines also showed significantly less disease symptoms compared to the untransformed controls. To assess the mechanism of resistance, we inoculated plants with DON and examined the concentration of DON and D3G from 1-21 days after inoculation. HvUGT13248-overexpressing wheat plants converted DON to D3G more rapidly to a higher extent than untransformed plants. We also extended our exploration to the function of HvUGT13248 gene toward NIV, and found that HvUGT13248-overexpressing wheat lines exhibits high level of type II resistance to a NIV-producing Fusarium graminearum strain.


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