U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Avian Disease and Oncology Research: Athens, GA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Richard B Russell Center
                                    Southeast Poultry Research Center
                                    Return to USNPRC main page
                    Quality and Safety Assessment Research
                                    Poultry Microbiological Safety and Processing Research
                                    Toxicology and Mycotoxin Research
                                    Egg and Poultry Production Safety Research Unit
                                    Exotic and Emerging Avian Viral Diseases
                                    Endemic Poultry Viral Diseases Research
                                    Avian Disease and Oncology Research
                                    Return to main USNPRC pageSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Southeast Area Athens, Georgia U.S. National Poultry Research Center Avian Disease and Oncology Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            LEE, MI OK - Texas A&M Universityitem
                                            YANG, ENCE - Texas A&M Universityitem
                                            MORRISON, MIREILLE - Institut National De La Recherche Agronomique (INRA)item
                                            VIGNAL, ALAIN - Institut National De La Recherche Agronomique (INRA)item
                                            HUANG, YONG-ZHEN - Northwest Agricultural & Forestry Universityitem
                                            Cheng, Hansitem
                                            MUIR, WILLIAM - Purdue Universityitem
                                            LAMONT, SUSAN - Iowa State Universityitem
                                            Lillehoj, Hyunitem
                                            Lee, Sungitem
                                            WOMACK, JAMES - Texas A&M University
  Submitted to: Genetic Selection Evolution
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 5/13/2014
                                    Publication  Date: 7/7/2014
                                    Publication URL: https://handle.nal.usda.gov/10113/59540Citation: Lee, M., Yang, E., Morrison, M., Vignal, A., Huang, Y., Cheng, H.H., Muir, W.M., Lamont, S.J., Lillehoj, H.S., Lee, S.H., Womack, J.E. 2014. Mapping and genotypic analysis of NK-lysin gene in chicken. Genetic Selection Evolution. 46:43. Available: http://www.gsejournal.org/content/46/1/43.Interpretive Summary: Molecular genetic characterization of biological relevant genes is become more common with genome assemblies, such those in livestock species including chicken. NK lysin is known to encode an important anti-microbial peptide with known variants that influence this activity. In this study, the variant was examined in a large number of chicken breeds in an effort to characterize the frequency in populations and to potentially correlate with anti-microbial activity. This information will help in genetic selection of disease resistance in a variety of chicken strains.
                                Technical Abstract: Background: Antimicrobial peptides (AMP) are important elements of the first line of defence against pathogens in animals. NK-lysin is a cationic AMP that plays a critical role in innate immunity. The chicken NK-lysin gene has been cloned and its antimicrobial and anticancer activity has been described but its location in the chicken genome remains unknown. Here, we mapped the NK-lysin gene and examined the distribution of a functionally significant single nucleotide polymorphism (SNP) among different chicken inbred lines and heritage breeds.

Results: A 6000 rad radiation hybrid panel (ChickRH6) was used to map the NK-lysin gene to the distal end of chromosome 22. Two additional genes, the adipocyte enhancer-binding protein 1-like gene (AEBP1) and the DNA polymerase delta subunit 2-like (POLD2) gene, are located in the same NW_003779909 contig as NK-lysin, and were thus indirectly mapped to chromosome 22 as well. Previously, we reported a functionally significant SNP at position 271 of the NK-lysin coding sequence in two different chicken breeds. Here, we examined this SNP and found that the A allele appears to be more common than the G allele in these heritage breeds and inbred lines.

Conclusions: The chicken NK-lysin gene mapped to the distal end of chromosome 22. Two additional genes, AEBP1 and POLD2, were indirectly mapped to chromosome 22 also. SNP analyses revealed that the A allele, which encodes a peptide with a higher antimicrobial activity, is more common than the G allele in our tested inbred lines and heritage breeds.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov