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ARS Home Plains Area Lincoln, Nebraska Wheat, Sorghum and Forage Research Research Publications at this Location





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Title:   Author  item
                                            Vogel, Kennethitem
                                            Sarath, Gautamitem
                                            Mitchell, Robert

                                                         - *Rob*
  Submitted to: Crop Science
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 1/15/2014
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary: Switchgrass is a perennial grass that is being developed into a biomass energy crop.  It is necessary to make controlled crosses between switchgrass plants to make improvements by plant breeding. Pollination bags are necessary to restrict pollen to those plants being inter-mated.  An improved pollination bag was made using a polyester micro-mesh fabric with a mesh size of 41 microns which is smaller than the mean reported 43 micron diameter of switchgrass pollen.  When used in paired plant crosses between switchgrass plants, the average amount of seed produced per plant was 800 seeds which was significantly greater than the 10 to 250 seeds produced previously in crosses of switchgrass plants using paper pollination bags.  The four to tenfold increase in seed produced per cross enables the progeny of the crosses to be evaluated in larger numbers in genetic studies and in replicated evaluation trials.
                                Technical Abstract: Pollination bags for making controlled crosses between switchgrass plants were made from a polyester micro-mesh fabric with a mesh size of 41 μm which is smaller than the mean reported 43 μm diameter of switchgrass pollen.  When used in paired plant crosses between switchgrass plants, the mean amount of seed produced per plant was about 1 gm or approximately 800 seeds which was significantly greater than the 10 to 250 seeds produced previously in paired plant crosses of switchgrass using paper pollination bags.  The four to tenfold increase in seed produced per cross enables the progeny of the crosses to be evaluated in larger numbers in genetic studies and in replicated space-transplanted or seeded sward evaluation trials.  The micro-mesh pollination bags cost about $15 each because of fabric costs but can be reused indefinitely.


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