U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Chemistry Research: Gainesville, FL
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    CMAVESearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Southeast Area Gainesville, Florida Center for Medical, Agricultural and Veterinary Entomology Chemistry Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            CABRERA, ANA - University Of Floridaitem
                                            Shirk, Paulitem
                                            Evans, Jayitem
                                            Teal, Peter
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 8/18/2013
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: Biosynthesis of 20-hydroxyecdysone (20E) in insects involves the action of five cytochrome P450s collectively known as Halloween genes. Transcripts from 3 Halloween genes [spook (Vdspo), disembodied (Vddib) and shade (Vdshd)] from the varroa mite were identified, sequenced and mapped to their genomic sequences. Predicted proteins from the coding regions of Vdspo, Vddib and Vdsh shared 33.3, 32.1 and 29.6% identity with their orthologs from Drosophila melanogaster, respectively. The Vdspo transcript was observed in gut preparations, while Vddib was present in the ovary/lyrate organ and Vdshd was present in ovary/lyrate organ, Malpighian tubules and gut samples. Transcript levels between phoretic mites and early reproductive mites, from pre-capping brood cells, were not significantly different for Vdspo. However, Vddib and Vdshd were significantly up-regulated by 87 and 105%, respectively. A cell invasion assay showed transcript levels from Vdspo, Vddib and Vdshd were not significantly different between mites that entered a brood cell within 4 hr compared to mites that remained on adult bees. These results suggest that the expression of Vddib and Vdshd does not direct the varroa mite cell invasion behavior, but is associated with the physiological shift from phoretic to reproductive state.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov