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Title:   Author  item
                                            REISCH, BRUCE - Cornell Universityitem
                                            Cadle-Davidson, Lanceitem
                                            Londo, Jasonitem
                                            TAKACS, ELIZABETH - Cornell Universityitem
                                            HYMA, KATIE - Cornell Universityitem
                                            SUN, QI - Cornell Universityitem
                                            BARBA, PAOLA - Cornell Universityitem
                                            FENNELL, ANNE - South Dakota State University
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 1/17/2014
                                    Publication  Date: 4/1/2014
                                    Citation: Reisch, B., Cadle Davidson, L.E., Londo, J.P., Takacs, E., Hyma, K., Sun, Q., Barba, P., Fennell, A. 2014. VitisGen: A coordinated effort in grapevine genotyping, phenotyping, and marker assisted breeding. International Symposium of Grapevine Physiology and Biotechnology, Serena, Chile.Interpretive Summary:
                                Technical Abstract: To enable early selection of elite seedlings combining disease resistance, stress tolerance, and fruit quality, U.S. grape breeders and geneticists developed a coordinated strategy for marker discovery and application using centralized phenotyping and map development via genotyping-by-sequencing (GBS).  This 5-year project (“VitisGen”) began in 2011, and 7,000 breeding lines were genotyped in the first year to track alleles introgressed from twelve Vitis species.  Our current GBS pipeline results in the mapping of over 20,000 SNPs per population using algorithms suitable for double pseudo-testcross populations. These dense genetic maps combined with the centralized phenotyping approach will lead to the development of at least 30 marker sets for alleles controlling traits of current and future interest for public grape breeding programs.  Precision phenotyping is coordinated through separate centers focusing on powdery mildew resistance, low temperature response, and fruit quality.  Breeders are phenotyping additional traits in 19 mapping populations with the goal of using all project phenotyping data to locate QTLs associated with a range of viticulturally important traits.  The VitisGen project also provides breeders with annual access to marker-assisted selection procedures, initially focusing on previously published SSR markers for disease resistance, flower sex, and other traits.


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