U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Meat Safety and Quality: Clay Center, NE
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    USMARC Home
                    USMARC Research Units
                        Genetics and Animal Breeding
                                    Livestock Bio Systems
                                    Nutrition, Growth and Physiology
                                    Animal Health GenomicsSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Plains Area Clay Center, Nebraska U.S. Meat Animal Research Center Meat Safety and Quality Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications
- IACUC Summaries

Title:   Author  item
                                            KIM, M - Former ARS Employeeitem
                                            Kuehn, Larryitem
                                            Bono, James

                                                         - *Jim*item
                                            Berry, Elaineitem
                                            Kalchayanand, Norasak

                                                         - *Nor*item
                                            Freetly, Harveyitem
                                            BENSON, A - University Of Nebraskaitem
                                            Wells, James

                                                         - *Jim*
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 3/10/2015
                                    Publication  Date: 4/13/2015
                                    Citation: Kim, M., Kuehn, L.A., Bono, J.L., Berry, E.D., Kalchayanand, N., Freetly, H.C., Benson, A.K., Wells, J. 2015. The effect of bovine fecal microbiome on Escherichia coli O157:H7 prevalence [abstract]. 2015 Congress on Gastrointestinal Function, 2015 April 13-15, Chicago, IL. p. 34.Interpretive Summary:
                                Technical Abstract: The objective of this study was to determine if fecal microbiome would have an association on E. coli O157:H7 prevalence. Pyrosequencing analysis of fecal microbiome was performed from feedlot cattle fed one of three diets: i) 94 heifers fed low concentrate (LC) diet, ii) 142 steers fed moderate concentrate (MC) diet, and iii) 132 steers fed high concentrate (HC) diet. A total of 2,411,122 high-quality sequences were obtained from 368 samples. In the LC diet group, 402,080 of the total sequences were recovered from 94 fecal samples and classified to 29 phyla. Firmicutes, candidate division TM7, Bacteroidetes, Tenericutes, Proteobacteria, Verrucomicrobia and Actinobacteria were core measurable phyla detected across almost all the 94 fecal samples. In the MC diet group, 1,035,186 of the total sequences were recovered from 142 fecal samples and classified to 22 phyla. Firmicutes, Bacteroidetes, Proteobacteria, Tenericutes, TM7, Cyanobacteria and Actinobacteria were core measurable phyla detected across almost all the 142 samples. In the HC group, 973,856 of the total sequences were recovered from 132 fecal samples and assigned to 26 phyla. Firmicutes, Bacteroidetes, Tenericutes, Proteobacteria, Actinobacteria, Cyanobacteria and TM7 were core measurable phyla detected across almost all the 132 samples. The 2,411,122 sequences were clustered into 322,585 OTUs at 97% sequence similarity. Stepwise regression with backward elimination indicated that 6, 15 and 10 OTUs brought significant information to each model (P < 0.0001) and explained 29, 51 and 33% of E. coli O157:H7 prevalence for each diet group, respectively. Research efforts would need to be made to isolate and characterize species corresponding to these OTUs correlated with E. coli O157:H7 prevalence.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov