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Title:   Author  item
                                            Hughes, Stephenitem
                                            Dowd, Patrickitem
                                            Johnson, Eric
  Submitted to: Journal of Pharmaceutical Sciences
                                    Publication Type: Other
                                    Publication Acceptance Date: 9/21/2012
                                    Publication  Date: 9/28/2012
                                    Citation: Hughes, S.R., Dowd, P.F., Johnson, E.T. 2012. Cell-penetrating recombinant peptides for potential use in agricultural pest control applications. Journal of Pharmaceutical Sciences. 5:1054-1063.Interpretive Summary:
                                Technical Abstract: Several important areas of interest intersect in a class of peptides characterized by their highly cationic and partly hydrophobic structure.  These molecules have been called cell-penetrating peptides (CPPs) because they possess the ability to translocate across cell membranes.  This ability makes these peptides attractive candidates for delivery of therapeutic compounds, especially to the interior of cells.  Compounds with characteristics similar to CPPs and that, in addition, have antimicrobial properties are being investigated as antibiotics with a reduced risk of developing resistance.  These membrane-acting antimicrobial peptides (MAMPs) are a-helical amphipathic peptides that interact with and perturb cell membranes to produce their antimicrobial effects.  One source of MAMPs is spider venom.  Because these compounds are toxic to insects, they also show promise for development as biological agents for control of insecticide-resistant agricultural pests.

Spider venom is a potential source of novel insect-specific peptide toxins.  One example is the small amphipathic a-helical peptide lycotoxin-1 (Lyt-1 or LCTX) from the wolf spider (Lycosa carolinensis).  One side of the a-helix has mostly hydrophilic and the other mainly hydrophobic amino acid residues.  The positive charge of the hydrophilic side interacts with negatively charged prokaryotic membranes and the hydrophobic side associates with the membrane lipid bilayer to permeabilize it.  Because the surface of the exoskeleton, or cuticle, of an insect is highly hydrophobic, to repel water and dirt, it would be expected that amphipathic compounds could permeabilize it.  Mutagenized lycotoxin 1 peptides were produced and expressed in yeast cultures that were fed to armyworm larvae to identify the most lethal mutants.  Because these modified lycotoxin-1 peptides were found to kill a high proportion of armyworms, putative tobacco transformants expressing one of these variants were generated and screened for resistance to insect damage.  Expression of the variant was found to provide resistance to corn earworms and cigarette beetles.


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