U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 close



Research
                Research Home
                            National Programs
                            Research Projects
                            Scientific Manuscripts
                            International Engagement
                            Scientific Software/Models
                            Databases and Datasets
                            Office of Scientific Quality Review
            Media
                News & Features
                            Multimedia
                            Briefing Room
                            Down on the Farm
                            Press Room
                            Science in Your Shopping Cart
                            Scientific Discoveries
                            Tellus
                            Under the Microscope
            About ARS
                About ARS Home
                            Staff Directory
                            Labs and Research Centers (Map)
                            Headquarter Offices
                            Organizational Chart
                            Employee Services (REE Employees Only)
                            Office of Outreach, Diversity, and Equal Opportunity
            Work With Us
                Work With Us Home
                            ARS Vacancies at USAJOBS
                            Careers at ARS Information
                            Scientific Collaborations
                            Extramural Agreements
                            1890 Faculty Research Sabbatical ProgramSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Northeast Area Geneva, New York Plant Genetic Resources Unit (PGRU) Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            GARDNER, KYLE - Dalhousie Universityitem
                                            Schwaninger, Heidiitem
                                            DANN, SCOTT - Acadia Universityitem
                                            Baldo, Angelaitem
                                            Chao, Chihchengitem
                                            Fazio, Gennaroitem
                                            Volk, Gayleitem
                                            Richards, Christopheritem
                                            Zhong, Gan-Yuanitem
                                            MYLES, SEAN - Dalhousie University
  Submitted to: Annual International Plant & Animal Genome Conference
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 12/15/2012
                                    Publication  Date: 1/13/2013
                                    Citation: Gardner, K., Schwaninger, H.R., Dann, S., Baldo, A.M., Chao, C.T., Fazio, G., Volk, G.M., Richards, C.M., Zhong, G., Myles, S. 2013. Genome-wide survey of genetic diversity of apple using genotyping-by-sequencing [abstract]. Annual International Plant & Animal Genome Conference. W248.Interpretive Summary:
                                Technical Abstract: With the advent of next-generation sequencing technologies it is now possible to generate large numbers of genetic markers without the need to rely on costly microarray platforms. Genotyping-by-Sequencing (GBS) enables the simultaneous identification and genotyping of single nucleotide polymorphisms (SNPs) in a highly multiplexed sample of multiple individuals. To investigate the domestication and breeding history of apples and to provide a foundation for marker-assisted breeding, we have used GBS to genotype about 2000 wild and cultivated apples from the USDA apple germplasm collection. From about 700 Gbp of sequence data generated from 42 lanes of the Illumina Hi-Seq platform, we have identified over 1,000,000 SNPs in the apple genome. Using these data, we demonstrate that patterns of population structure are in agreement with known relationships among apple species and cultivars. In addition, we present results of a preliminary genome scan for selection to identify the loci targeted during apple domestication and breeding.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov