U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Cereal Disease Lab: St. Paul, MN
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Cereal Rust Bulletins
                    PublicationsSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area St. Paul, Minnesota Cereal Disease Lab Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            LIU, W - Kansas State Universityitem
                                            Jin, Yueitem
                                            Rouse, Matthew

                                                         - *Matt*item
                                            FRIEBE, B - Kansas State Universityitem
                                            GILL, B - Kansas State Universityitem
                                            PUMPHREY, M - Washington State University
  Submitted to: Theoretical and Applied Genetics
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 2/5/2011
                                    Publication  Date: 2/24/2011
                                    Citation: Liu, W., Pumphrey, M., Jin, Y., Rouse, M.N., Friebe, B., Gill, B. 2011. Development and characterization of wheat-Ae. searsii Robertsonian translocations and a recombinant chromosome conferring resistance to stem rust. Theoretical and Applied Genetics. 122:1537-1545.Interpretive Summary: Stem rust, caused by Puccinia graminis tritici, is one of the most damaging diseases of the wheat crop. The emergence of a new highly virulent race of stem rust, Ug99 and related variants, can overcome resistance of widely deployed genes. This stem rust race has spread towards important wheat-growing areas. Exploiting novel genes effective against Ug99 from wild relatives of wheat is one of the most promising strategies for the protection of the wheat crop. A new source of resistance to Ug99 was identified in a wild wheat, Aegilops searsii. For transferring this resistance gene into common wheat, we produced three double-monosomic chromosome populations and then applied integrated stem rust screening, molecular marker analysis, and cytogenetic analysis to identify resistant wheat-Ae. searsii Robertsonian translocation. Three Robertsonian translocations and one recombinant with stem rust resistance were identified and confirmed to be genetically compensating on the basis of genomic in situ hybridization, analysis of DNA markers, and C-banding. In addition, nine DNA markers were developed for marker-assisted selection of the resistant gene. Efforts to reduce potential linkage drag associated with chromosome of Ae. searsii are currently under way.
                                Technical Abstract: Stem rust, caused by Puccinia graminis tritici, is one of the most damaging diseases of the wheat crop. The emergence of a new highly virulent race of stem rust, Ug99, rapid evolution of new Ug99 derivative races overcoming resistance of widely deployed genes, and spread towards important wheat-growing areas now potentially threaten world food security. Exploiting novel genes effective against Ug99 from wild relatives of wheat is one of the most promising strategies for the protection of the wheat crop. A new source of resistance to Ug99 was identified in the short arm of the Aegilops searsii chromosome 3Ss by screening wheat-Ae. searsii introgression libraries available as individual chromosome and chromosome arm additions to the wheat genome. For transferring this resistance gene into common wheat, we produced three double-monosomic chromosome populations (3A/3Ss, 3B/3Ss, and 3D/3Ss) and then applied integrated stem rust screening, molecular marker analysis, and cytogenetic analysis to identify resistant wheat-Ae. searsii Robertsonian translocation. Three Robertsonian translocations (T3AL/3SsS, T3BL/3SsS, and T3DL/3SsS) and one recombinant (T3DS-3SsS/3SsL) with stem rust resistance were identified and confirmed to be genetically compensating on the basis of genomic in situ hybridization, analysis of 3A, 3B, 3D, and 3SsS-specific SSR/STS-PCR markers, and C-banding. In addition, nine SSR/STS-PCR markers of 3SsS-specific were developed for marker-assisted selection of the resistant gene. Efforts to reduce potential linkage drag associated with 3SsS of Ae. searsii are currently under way.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov