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ARS Home Northeast Area Beltsville, Maryland (BARC) Beltsville Agricultural Research Center Bee Research Laboratory Research Publications at this Location





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Title:   Author  item
                                            DE GRAAF, DIRK - Ghent Universityitem
                                            ALIPPI, ADRIANNA - National University Of Laplataitem
                                            ANTUNEZ, KARINA - University Of Montevideoitem
                                            Aronstein, Katherineitem
                                            BUDGE, GILES - Central Science Laboratoryitem
                                            DEKOKER, DIETER - Ghent Universityitem
                                            DE SMET, LINA - Ghent Universityitem
                                            DINGMAN, DOUGLAS - University Of Connecticutitem
                                            Evans, Jayitem
                                            FOSTER, LEONARD - University Of British Columbiaitem
                                            FUNFHAUS, ANNE - Institute For Bee Researchitem
                                            GARCIA-GONZALEZ, EVA - Institute For Bee Researchitem
                                            GREGORC, ALES - Agricultural Institute Of Sloveniaitem
                                            HUMAN, HANNELIE - University Of Pretoriaitem
                                            Murray, Keithitem
                                            NGUYEN, BACH KIM - University Of Liegeitem
                                            POPPINGA, LENA - Institute For Bee Researchitem
                                            SPIVAK, MARLA - University Of Minnesotaitem
                                            VANENGELSDORP, DENNIS - University Of Marylanditem
                                            WILKINS, SELWYN - Central Science Laboratoryitem
                                            GENERSCH, ELKE - Institute For Bee Research
  Submitted to: Journal of Apicultural Research
                                    Publication Type: Review Article
                                    Publication Acceptance Date: 12/1/2012
                                    Publication  Date: 4/1/2013
                                    Citation: De Graaf, D.C., Alippi, A.M., Antunez, K., Aronstein, K.A., Budge, G., Dekoker, D., De Smet, L., Dingman, D.W., Evans, J.D., Foster, L.J., Funfhaus, A., Garcia-Gonzalez, E., Gregorc, A., Human, H., Murray, K.D., Nguyen, B., Poppinga, L., Spivak, M., Vanengelsdorp, D., Wilkins, S., Genersch, E. 2013. Selected techniques and protocols in American foulbrood research. Journal of Apicultural Research. 52(1). doi: 10.3896/IBRA.1.52.1.11.Interpretive Summary: American foulbrood (AFB) is a tightly regulated bacterial disease of honey bees that affects trade, the availability of colonies for pollination, and the economic survival of beekeeper operations. This article provides current methods for identifying, studying, and eliminating the bacterium causing AFB. These methods are standardized so that work is comparable across labs, and so that all researchers and regulators can use the most up-to-date methods for controlling this disease.
                                Technical Abstract: American foulbrood is one of the most devastating diseases of the honey bee caused by the spore-forming, Gram-positive rod-shaped bacterium Paenibacillus larvae.  The recent updated genome assembly and annotation of this pathogen now permits in depth molecular studies.  In the present paper, selected techniques and protocols for American foulbrood research are provided, often in a bullet-style format that permits easy implementation in the laboratory.  Topics covered include: ‘working with Paenibacillus larvae’, ‘basic microbiological techniques’, ‘experimental infection’ and ‘omics and other sophisticated techniques’.  Further, this paper covers other technical information including ‘biosafety measurements’ to guarantee the safe handling of this pathogen and the ‘expression of heterologous proteins in P. larvae’.


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