U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Harry K. Dupree Stuttgart National Aquaculture Research Cntr: Stuttgart, AR
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Southeast Area Stuttgart, Arkansas Harry K. Dupree Stuttgart National Aquaculture Research Cntr Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications
- IACUC Summary

Title:   Author  item
                                            Farmer, Bradleyitem
                                            Straus, David

                                                         - *Dave*item
                                            Fuller, Adam
  Submitted to: Book of Abstracts Aquaculture America
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 9/23/2010
                                    Publication  Date: 3/2/2011
                                    Citation: Farmer, B.D., Straus, D.L., Fuller, S.A. 2011. Evaluation of a candidate control gene for Flavobacterium columnare expression analysis and expression profiles in four growth media [abstract]. Aquaculture America 2011: Aquaculture on Parade, February 28-March 3, 2011, New Orleans, Lousiana. p.147.Interpretive Summary:
                                Technical Abstract: Investigations into gene expression often yield important data that cannot be ascertained by other techniques.  Studies of this nature hinge strongly on the reference or control gene.  Primers and a fluorescent probe were designed to amplify the 16S ribosomal gene of Flavobacterium columnare.  The 16S primer/probe set was compared to the existing QPCR primer/probe set of the chondrotinase gene; both genes were amplified under identical conditions with identical components.  Results showed that both primer/probe sets had very similar efficiency (1.97 vs. 1.94) and cycle variation (CV).  Genes were expressed in media as well as in infected fish tissues.  Standard procedures for the RT PCR of the genes for basic relative expression ('' CT) has been tested using two isolates.  Gene expression was profiled after isolates were cultured in various media.  This data can be used to validate the 16S primer/probe set, stability of the control gene and optimal media.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov