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Title:   Author  item
                                            TRAINOR, ELIZABETH - Food And Drug Administration(FDA)item
                                            WARFEL, JASON - Food And Drug Administration(FDA)item
                                            Nicholson, Tracyitem
                                            KOESTLER, BENJAMIN - Michigan State Universityitem
                                            ZIMMERMAN, LINDSEY - Food And Drug Administration(FDA)item
                                            KELLY, VANESSA - Food And Drug Administration(FDA)item
                                            WATERS, CHRISTOPHER - Michigan State Universityitem
                                            MERKEL, TOD - Food And Drug Administration(FDA)
  Submitted to: American Society for Microbiology General Meeting
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 8/1/2014
                                    Publication  Date: 8/1/2014
                                    Citation: Trainor, E.A., Warfel, J.M., Nicholson, T.L., Koestler, B.J., Zimmerman, L.I., Kelly, V.K., Waters, C.M., Merkel, T.J. 2014. Expression of bvg-repressed genes in Bordetella pertussis is controlled by RisA through a novel c-di-GMP signaling pathway. 114th General Meeting of the American Society for Microbiology. Paper No. 728. p. 127.Interpretive Summary:
                                Technical Abstract: The BvgAS two component system of Bordetella pertussis controls virulence factor expression. In addition, BvgAS controls expression of the bvg-repressed genes through the action of the repressor, BvgR. The transcription factor RisA is inhibited by BvgR, and when BvgR is not expressed RisA induces the transcription of the bvg-repressed genes. RisA is an orphan response regulator in B. pertussis, and the mechanism by which RisA activity is regulated is unknown. We report here that RisA activity is controlled by a previously unidentified c-di-GMP signaling pathway. We identified a gene, designated risX, as an active diguanylate cyclase that is required for RisA activation. In addition, BvgR was demonstrated to be a putative phosphodiesterase suggesting BvgR modulates c-di-GMP. We also show that RisA is able to bind c-di-GMP directly and identified a novel set of risA-activated genes. Together, our results support a model whereby the B. pertussis orphan response regulator RisA activates transcription of the bvg-repressed genes through a novel c-di-GMP signaling pathway.


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