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ARS Home Midwest Area Ames, Iowa National Animal Disease Center Infectious Bacterial Diseases Research Research Publications at this Location





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Title:   Author  item
                                            NOL, P - Animal And Plant Health Inspection Services (APHIS), National Wildlife Centeritem
                                            ELLIS, C - Animal And Plant Health Inspection Services (APHIS), National Wildlife Centeritem
                                            STAHL, R - Animal And Plant Health Inspection Services (APHIS), National Wildlife Centeritem
                                            HAICK, H - Israel Institute Of Technologyitem
                                            RHYAN, J - Animal And Plant Health Inspection Services (APHIS), National Wildlife Centeritem
                                            VERCAUTEREN, K - Animal And Plant Health Inspection Services (APHIS), National Wildlife Centeritem
                                            MCCOLLUM, M - Animal And Plant Health Inspection Service (APHIS)item
                                            Waters, Wadeitem
                                            Palmer, Mitchellitem
                                            SALMAN, M - Colorado State University
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 5/1/2014
                                    Publication  Date: 6/16/2014
                                    Citation: Nol, P., Ellis, C.K., Stahl, R.S., Haick, H., Rhyan, J.C., Vercauteren, K.C., Mccollum, M.P., Waters, W.R., Palmer, M.V., Salman, M.D. 2014. Analysis of breath volatile organic compounds as a screening tool for detection of Tuberculosis in cattle [abstract]. Abstract No. 93.Interpretive Summary:
                                Technical Abstract: •	Keywords:  bovine tuberculosis; Mycobacterium bovis; breath analysis; volatile organic compound; gas chromatography; mass spectrometry; NaNose
•	Introduction:  This presentation describes two studies exploring the use of breath VOCs to identify Mycobacterium bovis infection in cattle. 
•	Methods:  
Study 1- breath samples were collected onto sorbent cartridges from ten M. bovis-positive cattle and four negative cattle from a naturally infected dairy in southern Colorado, USA.  Additionally, breath was collected from 13 negative cattle at a dairy in northern Colorado.  Samples were analyzed using gas chromatography/mass spectrometry (GC/MS).  A nanotechnology-based array of sensors (NaNose) was tailored based on the GC/MS results for detection of breath from M. bovis-infected cattle.  Sensor responses were used as inputs for a discriminant factor analysis pattern recognition algorithm.  
Study 2- Samples were analyzed using GC/MS.  Breath samples from 23 male dairy calves (7 non-infected and 16 M. bovis-infected) were collected 90 days post experimental inoculation with 104 cfu M. bovis.  Chromatographic data were analyzed using standard analytical chemical and metabolomic analyses, principle components analysis, and a linear discriminant algorithm.  
•	Results:  
Study 1-GC/MS analysis identified two VOCs associated with infection and two with apparently non-infected animals.  The NaNose identified all infected cattle with 21% false positives in the controls. 
Study 2- Linear discriminant analysis models based on ions identified as significantly different across treatment groups allowed for classification of M. bovis-infected cattle vs. controls.  
•	Conclusion:  The findings provide proof of concept that breath-derived volatile organic compound analysis can be used to differentiate between healthy and M. bovis-infected cattle.


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