U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Temperate Tree Fruit and Vegetable Research: Wapato, WA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Facilities
                        USDA Laboratory in Wapato, WA
                                    USDA Prosser Worksite
                                    USDA Moxee Farm
                    About UsSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Pacific West Area Wapato, Washington Temperate Tree Fruit and Vegetable Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Swisher Grimm, Kylieitem
                                            Crosslin, James
  Submitted to: Southwestern Entomologist
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 11/25/2013
                                    Publication  Date: 3/1/2014
                                    Citation: Swisher, K.D., Crosslin, J. 2014. Restriction digestion method for haplotyping the potato psyllid, Bactericera cockerelli. Southwestern Entomologist. 39:49-56.Interpretive Summary:   The potato psyllid is linked to economically-important diseases of solanaceous crops in North and Central America and New Zealand.  Previous research of this insect pest has identified 4 psyllid populations that correlate to different geographical regions.  The past research has predominantly used the high resolution melting technique to identify psyllid populations; a technique that is efficient, but requires costly equipment.   This current study identified a second technique, restriction enzyme digestion, for identifying psyllid populations.  This new method can be done at low cost by any laboratory with basic molecular biology equipment.  Additionally, this study identified a second target for determining psyllid populations, and supports the previous finding of four different psyllid populations.
                                Technical Abstract: A restriction digestion method has been developed for haplotyping the potato psyllid, Bactericera cockerelli Sulc., an economically important pest of solanaceous crops.  This method differentiates the four known potato psyllid haplotypes by utilizing restriction enzyme digestion of a portion of the mitochondrial large subunit ribosomal RNA, tRNA-Val, and a portion of the small subunit ribosomal RNA, providing a second target within the mitochondrial DNA for haplotyping studies.  This technique also provides a good alternative to the current method of potato psyllid haplotyping which uses high resolution melting analysis, a technique that requires access to a real-time PCR machine with high resolution melting capabilities.  Potato psyllid haplotyping by restriction digestion is done using basic laboratory equipment that is readily available for smaller laboratories with budgetary limitations, thereby providing an excellent tool for laboratories of all sizes to identify psyllid populations.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov