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Title:   Author  item
                                            MAGGIOLI, M - Iowa State Universityitem
                                            Palmer, Mitchellitem
                                            VORDERMEIER, H - Veterinary Laboratories Agency (VLA)item
                                            WHELAN, A - Veterinary Laboratories Agency (VLA)item
                                            Waters, Wade
  Submitted to: Meeting Proceedings
                                    Publication Type: Proceedings
                                    Publication Acceptance Date: 2/17/2014
                                    Publication  Date: 5/2/2014
                                    Citation: Maggioli, M.F., Palmer, M.V., Vordermeier, H.M., Whelan, A.O., Waters, W.R. 2014. Bovine central memory T cells are highly proliferative in response to bovine tuberculosis infection. Proceedings of American Association of Immunologists Annual Meeting. p. 22.Interpretive Summary:
                                Technical Abstract: Long-term (i.e., 14 days) cultured IFN-gamma ELISPOT assays measure central memory T cell (Tcm) responses in both humans and cattle. With bovine tuberculosis, vaccine-elicited long-term IFN-gamma ELISPOT responses correlate with protection. In other species, Tcm’s pose low activation threshold and are highly proliferative. It has been shown that Tcm’s accounts for 75% of long-term cultured cells. The objective of this study was to access the proliferative capability of Tcm cells compared to 6 day cultured cells (n = 6) in response to aerosol Mycobacterium bovis infection. Long-term cultured PBMCs from infected cattle were stimulated with M. bovis purified protein derivative (PPDb), rESAT-6:CFP10 (E:C), rTB10.4 and rAg85A for 13 days. Next, cells were stained with CellTrace dye and re-stimulated with E:C in the presence of fresh autologous adherent cells for an additional six days (20 days). In response to E:C, long-term cultured were mainly CD4+ cells and were more proliferative (~ 48%) than 6d cultured cells (27%; p=0.001). Long-term cultured cells were: ~30% Tcm (CCR7+, CD45RO+), ~48% Tem (CCR7-, CD45RO+), ~8% effector (CCR7-, CD45RO-) and <1% of naïve cells (CCR7+, CD45RO-). Six day cultured cells were: ~16% Tcm (p=0.01), ~47% Tem, ~42% effector (p=0.001) and < 1% naïve cells. These findings demonstrate that bovine Tcm cells present high proliferative capability as compared to short-term cultured cells.


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