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Title:   Author  item
                                            Shahab-Ferdows, Settiitem
                                            ANAYA-LOYOLA, MIRIAM - Autonomous University Of Querétaroitem
                                            VERGARA-CASTANEDA, HAYDE - Autonomous University Of Querétaroitem
                                            ROSADO, JORGE L - Autonomous University Of Querétaroitem
                                            Keyes, Williamitem
                                            Newman, Johnitem
                                            MILLER, JOSHUA - University Of Californiaitem
                                            Allen, Lindsay

                                                         - *A*
  Submitted to: Journal of Nutrition
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 6/19/2012
                                    Publication  Date: 6/12/2012
                                    Citation: Shahab-Ferdows, S., Anaya-Loyola, M.A., Vergara-Castaneda, H., Rosado, J., Keyes, W.R., Newman, J.W., Miller, J., Allen, L.H. 5012. Vitamin B-12 supplementation of rural Mexican women changes biochemical B-12 status indicators but does not affect hematology or a bone turnover marker. Journal of Nutrition. vol. 142, no. 10, 1881-1887.Interpretive Summary: Based on the high prevalence of low serum vitamin B-12 concentrations and low dietary intake of the vitamin in Latin American studies including research in Mexico, it appears that vitamin B-12 deficiency is common. Whether this is associated with adverse effects on human function is unknown. To evaluate this question, a randomized controlled trial was conducted in rural Queretaro, Mexico. Women age 20-59 y (n=132) were assigned at baseline to deficient, marginal and adequate status groups based on their serum vitamin B-12 (75-148, 149-220, and >220 pmol/L, respectively). Those with serum B-12 <75 pmol/L (6%) were all treated with B-12 and not studied further. Women in each group were then randomized to receive vitamin B-12 (a 1 mg intramuscular dose followed by 500 mg supplements for 3 mo, n=70) or a placebo (n=62). Blood samples were collected at baseline and after the 3 mo intervention. At baseline 11% of the women were deficient and 22% had marginal status. Other biomarkers of B-12 deficiency at baseline were holoTC (low in 23%), methylmalonic acid (elevated in 21%) and homocysteine (elevated in 31%). All three biomarkers were correlated with serum B-12. Supplementation with vitamin B-12 brought all biomarker values to normal except for homocysteine which remained elevated in 21%, possibly due to other B vitamin deficiencies. Supplementation did not improve hemoglobin or any other red blood cell parameters, or affect bone turnover as measured by bone specific alkaline phosphatase. We conclude that vitamin B-12 status is poor in these women and that supplementation improved their enzyme function as reflected in normalization of biomarkers, but assessing their hematological status and a bone turnover marker did not reveal other functional abnormalities.
                                Technical Abstract: Based on the high prevalence of low serum vitamin B-12 concentrations and low dietary intake of the vitamin in Latin American studies including research in Mexico, it appears that vitamin B-12 deficiency is common. Whether this is associated with adverse effects on human function is unknown. To evaluate this question, a randomized controlled trial was conducted in rural Queretaro, Mexico. Women age 20-59 y (n=132) were assigned at baseline to deficient, marginal and adequate status groups based on their serum vitamin B-12 (75-148, 149-220, and >220 pmol/L, respectively). Those with serum B-12 <75 pmol/L (6%) were all treated with B-12 and not studied further. Women in each group were then randomized to receive vitamin B-12 (a 1 mg intramuscular dose followed by 500 mg supplements for 3 mo, n=70) or a placebo (n=62). Blood samples were collected at baseline and after the 3 mo intervention. At baseline 11% of the women were deficient and 22% had marginal status. Other biomarkers of B-12 deficiency at baseline were holoTC (low in 23%), methylmalonic acid (elevated in 21%) and homocysteine (elevated in 31%). All three biomarkers were correlated with serum B-12. Supplementation with vitamin B-12 brought all biomarker values to normal except for homocysteine which remained elevated in 21%, possibly due to other B vitamin deficiencies. Supplementation did not improve hemoglobin or any other red blood cell parameters, or affect bone turnover as measured by bone specific alkaline phosphatase. We conclude that vitamin B-12 status is poor in these women and that supplementation improved their enzyme function as reflected in normalization of biomarkers, but assessing their hematological status and a bone turnover marker did not reveal other functional abnormalities.


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