U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Virus and Prion Research: Ames, IA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area Ames, Iowa National Animal Disease Center Virus and Prion Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Kitikoon, Pravinaitem
                                            Baker, Amy
  Submitted to: Methods in Molecular Biology
                                    Publication Type: Book / Chapter
                                    Publication Acceptance Date: 3/10/2014
                                    Publication  Date: 6/30/2014
                                    Citation: Kitikoon, P., Vincent, A.L. 2014. Microneutralization assay for swine influenza virus in swine serum. In: Spackman, E., editor. Methods in Molecular Biology, Animal Influenza Virus. 2nd edition. New York, NY: Springer. p. 325-335.Interpretive Summary:
                                Technical Abstract: The microneutralization (MN) assay is a modification of the serum virus neutralization assay and is a serological test to detect the presence of functional systemic antibodies that prevent infectivity of virus. When infectious virus is mixed with serum antibody, the virus infectivity can be "neutralized" if the antibodies bind to blocking epitopes on the virus. The neutralization effect can be demonstrated by inoculation of susceptible cells or organisms with the antibody-virus mixture, such as cells in culture, embryonated eggs or susceptible hosts. The MN assay described here has a read out based on cell culture in a microtiter plate format and a color change system detected by an automated plate reader. The test is performed with a constant amount of virus and serial dilutions of serum samples to an end point where virus neutralization is not detected. The neutralizing antibody titer is thus the reciprocal number of the last dilution of serum with neutralizing activity. The MN assay can be used to detect antibody from pigs with natural exposure or vaccination and can potentially be used to predict cross-protection between strains of IAV.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov