U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Animal Parasitic Diseases Laboratory: Beltsville, MD
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Resources
                        Our ScientistsSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Northeast Area Beltsville, Maryland (BARC) Beltsville Agricultural Research Center Animal Parasitic Diseases Laboratory Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Jenkins, Markitem
                                            Fetterer, Raymonditem
                                            Miska, Kate
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 6/9/2013
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: A hallmark of Eimeria infection in avians is the establishment of immunity against clinical signs of coccidiosis.  Resistant birds experience improved weight gain and feed conversion efficiency and lack intestinal lesions.  Oocysts excretion is reduced, but not eliminated, in such immune chickens.  The purpose of this study was to determine whether the immune status of chickens influenced the expression of an immunodominant refractile body antigen in sporozoites of Eimeria acervulina.  To do so, sporozoite DNA and RNA was isolated after primary and 3 secondary challenges and subjected to PCR and RT-PCR amplification of a sequence coding for an immunodominant refractile body (RB) protein.  DNA sequencing of clones from these amplifications revealed polymorphism (3 isoforms) in the repeat region of the RB protein, such that a stop codon was introduced into the sequence causing a premature termination of RNA transcription (100 bp upstream).  All 3 isoforms were expressed in Escherichia coli, and antisera were prepared against full-length and truncated proteins.  Immunofluorescence staining of E. acervulina sporozoites with these sera revealed strong recognition of the mature, full length RB isoform, but no staining of the truncated isoforms.  While preliminary data suggest differential expression of these isoforms in immune vs. naïve chickens, more research is necessary to confirm this finding, and to determine if, within populations of E. acervulina sporozoites, different isoforms generally coexist. It also remains to be determined whether immune pressure is necessary and sufficient to engender differential expression of these isoforms.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov