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Title:   Author  item
                                            Kitikoon, Pravinaitem
                                            Loving, Crystalitem
                                            Baker, Amy
  Submitted to: Methods in Molecular Biology
                                    Publication Type: Book / Chapter
                                    Publication Acceptance Date: 3/10/2014
                                    Publication  Date: 6/30/2014
                                    Citation: Kitikoon, P., Loving, C.L., Vincent, A.L. 2014. Antibody secreting cell assay for influenza A virus in swine. In: Spackman, E., editor. Methods in Molecular Biology, Animal Influenza Virus. 2nd edition. New York, NY: Springer. p. 347-353.Interpretive Summary:
                                Technical Abstract: An ELISPOT assay to enumerate B-cells producing antibodies specific to a given antigen, also known as an antibody secreting cell (ASC) assay, was adapted to detect B-cells specific for influenza A virus (IAV). The assay is performed ex vivo and enumerates ASC at a single cell level. A simple ASC detection method is based on a solid phase immunoenzymatic principle. The ELISPOT plate is coated with IAV prior to incubation with cell samples. The spot-forming cells are detected by enzyme labeled antibodies directed to the swine immunoglobulin (Ig) class of interest and visualized with the addition of substrate.


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