U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Meat Safety and Quality: Clay Center, NE
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    USMARC Home
                    USMARC Research Units
                        Genetics and Animal Breeding
                                    Livestock Bio Systems
                                    Nutrition, Growth and Physiology
                                    Animal Health GenomicsSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Plains Area Clay Center, Nebraska U.S. Meat Animal Research Center Meat Safety and Quality Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications
- IACUC Summaries

Title:   Author  item
                                            Kim, Minitem
                                            KIM, JAEHYOUNG - University Of Nebraskaitem
                                            Kuehn, Larryitem
                                            Bono, James

                                                         - *Jim*item
                                            Berry, Elaineitem
                                            Kalchayanand, Norasak

                                                         - *Nor*item
                                            Freetly, Harveyitem
                                            BENSON, A - University Of Nebraskaitem
                                            Wells, James

                                                         - *Jim*
  Submitted to: Journal of Animal Science
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 11/19/2013
                                    Publication  Date: 2/12/2014
                                    Publication URL: http://handle.nal.usda.gov/10113/58466Citation: Kim, M.S., Kim, J., Kuehn, L.A., Bono, J.L., Berry, E.D., Kalchayanand, N., Freetly, H.C., Benson, A.K., Wells, J. 2014. Investigation of bacterial diversity in the feces of cattle fed different diets. Journal of Animal Science. 92:683-694.Interpretive Summary: Recent research has indicated that diet may greatly influence the shedding of E. coli O157:H7, however little is known about the influence of diet on other bacteria in the gastrointestinal tract.  Fecal samples were collected from 290 feedlot cattle fed finishing diets with high levels of corn or moderate levels of corn and from 136 cattle fed a forage-based diet.  Compositions of the bacteria in the feces were determined using modern DNA sequencing methods.  Within a dietary group, the compositions of the bacteria were similar across multiple animals.  Between the dietary groups, the composition of bacteria was different due to diet.  Forage had a greater influence on the differences than did the level of corn in the diet.  From this study, it appears that the bovine diet can greatly influence the composition of the bacteria in the gastrointestinal tract and feces.  Understanding the influence of indigenous microflora on the colonization and shedding of E. coli O157:H7 will provide a possible avenue for intervention in the preharvest beef production environment not yet exploited.
                                Technical Abstract: The objective of this study is to investigate individual animal variation of bovine fecal microbiota including as affected by diets. Fecal samples were collected from 426 cattle fed 1 of 3 diets typically fed to feedlot cattle: 1) 143 steers fed finishing diet (83% dryrolled corn, 13% corn silage, and 4% supplement), 2) 147 steers fed late growing diet (66% dry-rolled corn, 26% corn silage, and 8% supplement), and 3) 136 heifers fed early growing diet (70% corn silage and 30% alfalfa haylage). Bacterial 16S rRNA gene amplicons were determined from individual fecal samples using next-generation pyrosequencing technology. A total of 2,149,008 16S rRNA gene sequences from 333 cattle with at least 2,000 sequences were analyzed. Firmicutes and Bacteroidetes were dominant phyla in all fecal samples. At the genus level, Oscillibacter, Turicibacter, Roseburia, Fecalibacterium, Coprococcus, Clostridium, Prevotella, and Succinivibrio were represented by more than 1% of total sequences. However, numerous sequences could not be assigned to a known genus. Dominant unclassified groups were unclassified Ruminococcaceae and unclassified Lachnospiraceae that could be classified to a family but not to a genus. These dominant genera and unclassified groups differed (P < 0.001) with diets. A total of 176,692 operational taxonomic units (OTU) were identified in combination across all the 333 cattle. Only 2,359 OTU were shared across 3 diet groups. UniFrac analysis showed that bacterial communities in cattle feces were greatly affected by dietary differences. This study indicates that the community structure of fecal microbiota in cattle is greatly affected by diet, particularly between forage-and concentrate-based diets.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov