U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 close



Research
                Research Home
                            National Programs
                            Research Projects
                            Scientific Manuscripts
                            International Engagement
                            Scientific Software/Models
                            Databases and Datasets
                            Office of Scientific Quality Review
            Media
                News & Features
                            Multimedia
                            Briefing Room
                            Down on the Farm
                            Press Room
                            Science in Your Shopping Cart
                            Scientific Discoveries
                            Tellus
                            Under the Microscope
            About ARS
                About ARS Home
                            Staff Directory
                            Labs and Research Centers (Map)
                            Headquarter Offices
                            Organizational Chart
                            Employee Services (REE Employees Only)
                            Office of Outreach, Diversity, and Equal Opportunity
            Work With Us
                Work With Us Home
                            ARS Vacancies at USAJOBS
                            Careers at ARS Information
                            Scientific Collaborations
                            Extramural Agreements
                            1890 Faculty Research Sabbatical ProgramSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Lee, Sungitem
                                            Lillehoj, Hyunitem
                                            PARK, MYEONG SEON - US Department Of Agriculture (USDA)item
                                            BALDWIN, CYNTHIA - University Of Massachusettsitem
                                            TOMPKINS, DANNIELLE - University Of Massachusettsitem
                                            WAGNER, BETTINA - Cornell Universityitem
                                            BABU, UMA - Food And Drug Administration(FDA)item
                                            DEL CACHO, EMILIO - University Of Zaragozaitem
                                            MIN, WONGI - Gyeongsang National University
  Submitted to: Comparative Immunology Microbiology and Infectious Diseases
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 6/20/2011
                                    Publication  Date: 6/23/2011
                                    Citation: Lee, S.H., Lillehoj, H.S., Park, M., Baldwin, C., Tompkins, D., Wagner, B., Babu, U., Del Cacho, E., Min, W. 2011. Development and characterization of mouse monoclonal antibodies reactive with chicken CD80. Comparative Immunology Microbiology and Infectious Diseases. 34(3):273-279.Interpretive Summary: Limited availability of immune reagents that can be used to assay for poultry immunity hinders the progress in disease research in poultry. In this paper, ARS scientists developed mouse monoclonal antibodies which detect chicken cytokine CD80. CD80 is an important cell surface antigen on antigen presenting cells which is necessary for T-cell activation.  Ability to assess the expression and function of chicken CD80 will enhance basic and applied research on poultry immunology.  These mouse antibodies detecting chicken CD80 will be commercially available through technology transfer to Kingfisher company.
                                Technical Abstract: CD80 is one of the ligands for CD28 and is an important co-stimulator molecule on antigen presenting cells necessary for T-cell activation.  Although CD80 is well characterized in human, swine, ovine, feline, and canine species, there is no information on its chicken counterpart.  This study was carried out in order to develop immunological reagents that can be used to characterize chicken CD80 (chCD80). A recombinant plasmid of chCD80/horse IgG4 was constructed and expressed in CHO cells to produce recombinant chCD80 protein. Chicken CD80 was purified from the chCD80/IgG4 fusion protein by enterokinase digestion, and used to immunize BALB/c mice, resulting in 158 hybridomas that produced monoclonal Abs (mAbs) against chCD80. Three hybridomas that produced mouse mAbs with high binding specificity for rchCD80-transfected CHO cells were selected by flow cytometric analysis for further characterization. All mAbs to chCD80 showed staining of bursa and spleen, and identified a 80 kDa protein on CD80/IgG4-transfected cells in Western blot. Immunohistochemistry of lymphoid tissues revealed that CD80-expressing cells were expressed exclusively in the bursal follicles at the outer portion of the cortex in the bursa, and throughout the red pulp and the outer boundary of the white pulp in the spleen.  Addition of chCD80 mAb to Con A-stimulated spleen cells inhibited the percentage of MHCII-expressing cells and suppressed IL-2 induced lymphocyte proliferation (P < 0.05), making these CD80 specific mAbs valuable immunological reagents for basic and applied poultry immunology research.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov