U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Animal Biosciences & Biotechnology Laboratory: Beltsville, MD
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Announcements
                        ABBL Journal Club
                    USDA Civil Rights Policy StatementSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Northeast Area Beltsville, Maryland (BARC) Beltsville Agricultural Research Center Animal Biosciences & Biotechnology Laboratory Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Lee, Sungitem
                                            Lillehoj, Hyunitem
                                            JEONG, MI SUN - US Department Of Agriculture (USDA)item
                                            DEL CACHO, EMILIO - US Department Of Agriculture (USDA)item
                                            MIN, WONGI MIN - Gyeongsang National Universityitem
                                            JEOUNG, HYEYOUNG - Animal, Plant And Fisheries Quarantine And Inspection Agency (QIA)item
                                            AN, DONGJJUN - Animal, Plant And Fisheries Quarantine And Inspection Agency (QIA)
  Submitted to: Veterinary Immunology and Immunopathology
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 6/1/2014
                                    Publication  Date: 7/18/2014
                                    Citation: Lee, S.H., Lillehoj, H.S., Jeong, M., Del Cacho, E., Min, W., Jeoung, H., An, D. 2014. Development and characterization of mouse monoclonal antibodies reactive with chicken IL1 Beta. Veterinary Immunology and Immunopathology. 93(9):2193-2198.Interpretive Summary: Limited availability of diagnostic reagents to assess immune responses in poultry and the lack of information on key molecules that regulate the immune response of poultry hinder rapid progress in poultry immunology and vaccine development.  In this study, ARS scientists collaborated with other scientists to produce an immune factor that identifies a chicken protein important for the initiation of the immune response to infectious agents in poultry.  Furthermore, this immune factor was used to develop a diagnostic reagent that can be used to assess the level and production of the immune response protein in chicken tissues and serum during various infections in poultry.  The results showed that the new diagnostic reagent not only detects the immune response protein but also can be used to examine the immune response, both which will be important to further study the chicken’s immunity.  The availability of this valuable immune reagent for poultry will enhance basic and applied immunology research for scientists worldwide.
                                Technical Abstract: Two mouse monoclonal antibodies (mAbs) specific for chicken interleukin-1 Beta (chIL-1 Beta) were produced and characterized. Both mAbs identified a 66.0 kDa recombinant protein expressed in Escherichia coli by Western blot analysis that corresponded to the expected molecular weight of a recombinant fusion protein containing the full-length 23.0 kDa chIL-1 Beta protein and a 43.0 kDa maltose binding protein tag. Immunohistochemical analysis identified cells producing endogenous chIL-1Beta in the caecal tonsils, bursa of Fabricius, and spleen. Purified recombinant chIL-1 Beta dose-dependently stimulated the proliferation and nitric oxide production by thymocytes and both activities were inhibited by co-incubation with the two chIL-1 Beta mAbs. These mAbs will be important immune reagents for future basic and applied poultry research.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov