U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Bioenergy Research: Peoria, IL
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    NCAUR Homepage
                    About BER
                        Mission Statement
                                    Strategic Goals
                                    Specialized Research ResourcesSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area Peoria, Illinois National Center for Agricultural Utilization Research Bioenergy Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            CHEN, MING-HSU - University Of Illinoisitem
                                            Bowman, Michaelitem
                                            Dien, Bruceitem
                                            RAUSCH, KENT - University Of Illinoisitem
                                            TUMBLESON, MICHAEL - University Of Illinoisitem
                                            SINGH, VIJAY - University Of Illinois
  Submitted to: Bioresource Technology
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 12/12/2013
                                    Publication  Date: 12/22/2013
                                    Citation: Chen, M.-H., Bowman, M.J., Dien, B.S., Rausch, K.D., Tumbleson, M.E., Singh, V. 2014. Autohydrolysis of Miscanthus x giganteus for the production of xylooligosaccharides (XOS): Kinetics, characterization and recovery. Bioresource Technology. 155:359-365.Interpretive Summary: Production of biofuels from cellulosic biomass promises to expand the supply of renewable transportation fuel in the near future.  A scarcity of coproducts is a commercial barrier to producing cellulosic ethanol; and a facility solely producing cellulosic ethanol will likely be unprofitable.  Potential candidates for coproduct production include the hemicellulose, which represents the second most abundant component of plant biomass after cellulose. Hemicelluloses can be converted to ethanol or into other products including short soluble carbohydrates called xylooligosaccharides (XOS).  There is growing interest in production of XOS based on their beneficial health effects when included in foods because of their functional properties as a soluble fiber and ability to lower cholesterol blood levels.  Miscanthus (Miscanthus x giganteus) is a warm season perennial grass that has gained attention as a potential bioenergy crop.  In the current research, optima conditions for producing and purifying of xylooligosaccharides (XOS) from Miscanthus were investigated.  Reaction conditions were optimized for production of XOS on the basis of yield and the XOS characterized for structural and chemical composition. XOS were recovered by adsorbing onto activated carbon and eluting with a stepwise gradient of aqueous ethanol. The results will be useful to farmers growing warm season bioenergy crops and commercial operators seeking to develop cellulosic biorefineries.
                                Technical Abstract: The optima conditions of production and purification of xylooligosaccharides (XOS) from Miscanthus x giganteus (MxG) were investigated. Using autohydrolysis, XOS were produced at 160, 180 and 200°C at 60, 20 and 5 min, respectively. XOS yield up to 13.5% (w/w) of initial biomass and 69.2% (w/w) of xylan were achieved. Results from HPAEC-PAD analysis revealed that X1 to X9 sugar oligomers were produced. Higher temperature and longer reaction time resulted in lower product molecular weight. The three optimum conditions had similar degrees of polymerization XOS. Using 10% activated carbon (w/v) with ethanol/water elution recovered 47.9% (w/w) of XOS from pretreated liquid phase. The XOS could be fractionated by degree of polymerization according to ethanol concentration in the ethanol/water elution. Most of the XOS were washed out in 30 and 50% ethanol/water (v/v) fractions. Recoveries of 91.8% xylobiose, 86.9% xylotriose, 66.3% xylotetrose, 56.2% xylopentose and 48.9% xylohexaose were observed in XOS.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov