U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Food Safety and Intervention Technologies Research: Wyndmoor, PA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    About ERRC
                        Greetings from the Director
                                    Directions/Lodging
                                    History and Accomplishments
                    Our Work
                        Biomass Pyrolysis Research
                                    IPMP 2013
                                    IPMP Dynamic Prediction
                                    IPMP Global Fit
                                    Pathogen Modeling Program (PMP)
                                    Predictive Microbiology Information Portal (PMIP)
                                    PoultryFARMSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Northeast Area Wyndmoor, Pennsylvania Eastern Regional Research Center Food Safety and Intervention Technologies Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            LOU, FANGFEI - The Ohio State Universityitem
                                            HUANG, PENGWEI - Children'S Hospital - Cincinnati, Ohioitem
                                            NEETOO, HUDAA - University Of Delawareitem
                                            Gurtler, Joshuaitem
                                            Niemira, Brendanitem
                                            CHEN, HAIQIANG - University Of Delawareitem
                                            JAING, XI - Children'S Hospital - Cincinnati, Ohioitem
                                            LI, JIANRONG - The Ohio State University
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 3/6/2012
                                    Publication  Date: 7/22/2012
                                    Citation: Lou, F., Huang, P., Neetoo, H., Gurtler, J., Niemira, B.A., Chen, H., Jaing, X., Li, J. 2012. High pressure inactivation of human norovirus-like particles: evidence that the capsid of human norovirus is highly pressure resistant. Meeting Abstract. IAFP Annual Meeting., Providence, Rhode Island, July 22-25, 2012., Volume 1, Page 1.Interpretive Summary:
                                Technical Abstract: High pressure processing (HPP) is a promising non-thermal technology to inactivate foodborne viruses. However, the effectiveness of HPP on inactivating human norovirus (HuNoV), the leading cause of acute gastroenteritis, is unknown because it cannot be propagated in cell culture. Therefore, developing a new model system to understand the survival of HuNoV is urgently needed. The objective of this study is to evaluate the stability of capsid of HuNoV to HPP using virus-like particles (VLPs) as a model.  HuNoV VLPs were treated at pressures ranging from 500 to 900 MPa at 4 deg C for various holding times (from 1 to 60 min). The effectiveness of HPP on disrupting HuNoV capsid was evaluated by analyzing the integrity of VLP structure, and the binding to its functional receptors, histo blood group antigens (HBGAs). We found that pressurization at 500-600 MPa, the pressure capable of completely inactivating HuNoV surrogates (such as murine norovirus, MNV; and feline calicivirus, FCV) in 2 min, was not sufficient to disrupt the structure and function of HuNoV VLPs even with a holding time of 60 min. The disrupting efficacy of HPP increased with the pressure level. The time required for the complete disruption of HuNoV VLPs at 700, 800, and 900 MPa was 30, 10, and 2 min, respectively. Moreover, HuNoV VLPs were more resistant to HPP in their ability to bind the type A than the types B and O HBGAs. Additionally, the 23-nm VLPs appeared to be much more stable than the 38-nm VLPs. While HuNoV VLP is highly resistant to HPP, a level of 800-900MPa is capable of effectively disrupting HuNoV capsid within a short holding time. The apparent high resistance of HuNoV capsid to HPP also indicates that the HuNoV VLPs represents a better model than MNV and FCV to study the survival of HuNoV under various conditions. Better models will facilitate the use of HPP to inactivate pathogenic viruses, thereby improving the safety of shellfish and other foods.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov