U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Virus and Prion Research: Ames, IA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area Ames, Iowa National Animal Disease Center Virus and Prion Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            REDFERN, CRYSTAL - Wake Forest School Of Medicineitem
                                            GANGULY, TRIDIB - Wake Forest School Of Medicineitem
                                            CONOVER, MATT - Wake Forest School Of Medicineitem
                                            Nicholson, Tracyitem
                                            DEORA, RAJENDAR - Wake Forest School Of Medicine
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 8/15/2013
                                    Publication  Date: 9/8/2013
                                    Citation: Redfern, C., Ganguly, T., Conover, M.S., Nicholson, T.L., Deora, R. 2013. BpsR functions as a dual activator and repressor of Bordetella gene expression. 10th International Symposium on Bordetella. p. 46.Interpretive Summary:
                                Technical Abstract: Bordetella bacteria are Gram-negative respiratory pathogens of animals, birds, and humans. A hallmark feature of some Bordetella species is their ability to efficiently survive in the respiratory tract even after vaccination. Our overall hypothesis is that establishment of colonization and persistence is enhanced by formation of biofilms in the respiratory tract. Bordetella bronchiseptica and Bordetella pertussis form biofilms on abiotic surfaces and in the mouse respiratory tract. The Bps exopolysaccharide is one of the critical determinants for biofilm formation and the survival of Bordetella in the murine respiratory tract. Regulatory pathways controlling biofilm development and polysaccharide gene expression are poorly understood in Bordetella. We previously identified, BpsR as a regulator of biofilm formation. By repressing the expression of Bps, BpsR inhibits biofilm development. By carrying out DNAse I footprinting assays, we have identified regions protected by BpsR on bpsA-D. The protected region harbors a highly unusual complex array of symmetry elements in the form of inverted, complementary inverted, mirror-like direct repeats and nine repeats of a loosely conserved hexameric sequence with a consensus G/C(A/T)4G/C. Single round in vitro transcription assays showed that BpsR is sufficient to repress transcription from the bpsA-D promoter. Transcriptome profiling revealed that BpsR functions as global regulator and can both activate and repress gene expression. The results from this study provide insight into regulation of large gene networks in which transcription factors must play both positive and negative roles.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov