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Title:   Author  item
                                            CHEN, MING-HSU - University Of Illinoisitem
                                            RAJAN, KALAVATHY - University Of Arkansasitem
                                            Bowman, Michaelitem
                                            CARRIER, DANIELLE - University Of Arkansasitem
                                            Dien, Bruceitem
                                            RAUSCH, KENT - University Of Illinoisitem
                                            TUMBLESON, M - University Of Illinoisitem
                                            SINGH, VIJAY - University Of Illinois
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 9/29/2013
                                    Publication  Date: 9/29/2013
                                    Citation: Chen, M., Rajan, K., Bowman, M.J., Carrier, D.J., Dien, B.S., Rausch, K.D., Tumbleson, M.E., Singh, V. 2013. Purification and characterization of xylooligosaccharides (XOS) from Miscanthus x giganteus [abstract].Interpretive Summary:
                                Technical Abstract: Our previous investigation showed that xylooligosaccharides (XOS) could be produced effectively from Miscanthus x giganteus (MxG). Using autohydrolysis, an XOS yield of to 13.5% (w/w) of initial biomass and xylan yield of 69.2% (w/w) was observed. In this study, we investigated the purification of XOS from hydrolyzed liquid. An activated carbon adsorption procedure was set up for recovering XOS and was followed by elution at five different ethanol solution. Most of the XOS eluted out with 30%, 50% ethanol/water solution; highest recovery of 47.9% (w/w) of the initial XOS could be achieved. XOS with lower degree of polymer (DP) resulted in better recovery. Fractions from activated carbon elution had purity up to 75% (w/w). By using centrifugal partition chromatogram (CPC), we can isolate xylobiose (X2) and xylotriose (X3) at the purity of 64% and 65%, respectively. Further ESI-MS spectrums showed the major molecular weight of X2 are 305.1 (X2-Na) and 321.1(X2-K); major molecular weight of X3 are 437.1 (X3-Na) and 453.1 (X3-K).


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