U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Vegetable Crops Research: Madison, WI
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    ScientistsSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area Madison, Wisconsin Vegetable Crops Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Zalapa, Juanitem
                                            Steffan, Shawnitem
                                            Simon, Philippitem
                                            Senalik, Douglasitem
                                            Polashock, Jamesitem
                                            VORSA, NICHOLI - Rutgers Universityitem
                                            DIEGO, FAJARDO - University Of Wisconsinitem
                                            HARBUT, REBECCA - University Of Wisconsinitem
                                            ZHU, HUAYU - University Of Wisconsin
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 10/22/2011
                                    Publication  Date: 10/18/2011
                                    Citation: Zalapa, J.E., Steffan, S.A., Simon, P.W., Senalik, D.A., Polashock, J.J., Vorsa, N., Diego, F., Harbut, R., Zhu, H. 2011. Progress towards the complete American cranberry (Vaccinium macrocarpon Ait.) plastid genome [abstract]. Plant Sciences Symposium.Interpretive Summary:
                                Technical Abstract: Next generation sequencing (NGS) technology has been rapidly adopted for sequencing complete plant genomes due to its high-throughput and cost effective benefits. Advances towards the complete plastid genome sequence of the cranberry cultivar “HyRed” are presented using in silico procedures. We used previously generated “HyRed” 454 shotgun sequence data to isolate plastid data via homology comparisons with complete sequences from several species available at the National Center for Biotechnology (NCBI) database. Initially, forty cranberry plastid contigs, spanning a total length of 137,475 were discovered. After further analysis, 11 cranberry plastids scaffolds were constructed based on similarities with other plastid sequences at NCBI. From the cranberry plastid scaffolds, we reconstructed and annotation a putative cranberry plastid genome with an approximate length of 176kb. The development of cranberry plastid sequence using NGS technologies will allow the accumulation of critical genomic data useful for breeding and a suite of other genetic studies.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov