U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Crops Pathology and Genetics Research: Davis, CA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Outreach Events
                        UC Davis Picnic Day
                                    Kinders go to College
                                    STEM Girls
                                    Future Scientist Program
                                    Agriculture Future of America
                                    Wallace-Carver Fellowship
                                    Young Scholars Program
                                    Annual Awards Banquet
                    About Us
                        About Us
                                    Our Mission
                                    Principal InvestigatorsSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Pacific West Area Davis, California Crops Pathology and Genetics Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications
- Etiology and Biology of Tree Crop Diseases
- Rice Genetics
- Sustainable Floriculture
- Sustainable Viticulture

Title:   Author  item
                                            AL RWAHNIH, MAHER - University Of Californiaitem
                                            DAUBERT, STEVE - University Of Californiaitem
                                            Sudarshana, Mysoreitem
                                            ROWHANI, ADIB - University Of California
  Submitted to: Virus Genes
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 5/24/2013
                                    Publication  Date: 8/1/2013
                                    Publication URL: http://link.springer.com/article/10.1007/s11262-013-0921-3Citation: Al Rwahnih, M., Daubert, S.D., Sudarshana, M.R., Rowhani, A. 2013. Gene from a novel plant virus satellite from grapevine identifies a viral satellite lineage. Virus Genes. 47:114-118.Interpretive Summary: We have identified the genome of a novel viral satellite in deep sequence analysis of double-stranded RNA from grapevine.  The genome was 1,060 bases in length, and encoded two open reading frames.  Neither frame was related to any known plant virus gene.  But translation of the longer frame showed a protein sequence similar to those of other plant virus satellites.  Other than in commonalities they shared in this gene sequence, members of that group were extensively divergent.  The reading frame in this gene from the novel satellite could be translationally coupled to an adjacent reading frame in the -1 register, through overlapping start/stop codons.  These overlapping AUGA start/stop codons were adjacent to a sequence that could be folded into a pseudoknot structure.  Field surveys with PCR probes specific for the novel satellite revealed its presence in 3 % of the grapevines (n = 346) sampled.
                                Technical Abstract: We have identified the genome of a novel viral satellite in deep sequence analysis of double-stranded RNA from grapevine.  The genome was 1,060 bases in length, and encoded two open reading frames.  Neither frame was related to any known plant virus gene.  But translation of the longer frame showed a protein sequence similar to those of other plant virus satellites.  Other than in commonalities they shared in this gene sequence, members of that group were extensively divergent.  The reading frame in this gene from the novel satellite could be translationally coupled to an adjacent reading frame in the -1 register, through overlapping start/stop codons.  These overlapping AUGA start/stop codons were adjacent to a sequence that could be folded into a pseudoknot structure.  Field surveys with PCR probes specific for the novel satellite revealed its presence in 3 % of the grapevines (n = 346) sampled.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov