U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Virus and Prion Research: Ames, IA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area Ames, Iowa National Animal Disease Center Virus and Prion Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            SHAO, HONGXIA - University Of Marylanditem
                                            YE, JIANQIANG - University Of Marylanditem
                                            Baker, Amyitem
                                            SONG, HAICHEN - Synbiotics Corporationitem
                                            HICKMAN, DANIELLE - University Of Marylanditem
                                            QI, AIJIAN - Yangzhou Universityitem
                                            LAMICHHANE, CHINTA - Yangzhou Universityitem
                                            PEREZ, DANIEL - University Of Maryland
  Submitted to: Influenza and Other Respiratory Viruses
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 9/3/2010
                                    Publication  Date: 5/1/2011
                                    Citation: Shao, H., Ye, J., Vincent, A.L., Song, H., Hickman, D., Qi, A., Lamichhane, C., Perez, D.R. 2011. A monoclonal antibody-based ELISA for differential diagnosis of 2009 pandemic H1N1. Influenza and Other Respiratory Viruses. 5(Suppl.1):138-142.Interpretive Summary: Influenza A virus causes a respiratory disease in swine similar to that in humans. In addition, it is a component of the multiple pathogen porcine respiratory disease complex in young pigs. Inactivated vaccines work well when pigs are exposed to influenza viruses represented in the vaccine. However, as with the situation in humans, influenza viruses continuously evolve and eventually vaccine efficacy is reduced when new strains no longer cross-react with the immunity induced by the vaccine. This requires continuous monitoring of new viruses and updating of strains used in vaccines. In this report, we developed a monoclonal antibody against the hemagglutinin of the 2009 A/H1N1 pandemic strain and found that it possessed high cross-reactivity and neutralizing activity against multiple H1N1 pandemic strains, but also against classical swine and other H1 strains that belong to clusters circulating among US swine. To our knowledge, this is the first report demonstrating a monoclonal antibody with broad cross-reaction against multiple swine H1 influenza lineages. Based on the high neutralization titer of this antibody and its ability to protect mice against lethal 2009 A/H1N1 pandemic and prototypic swine H1 virus challenge after intranasal administration either before or after virus challenge, this antibody has potential use as an immune therapy against swine-lineage H1 influenza viruses.
                                Technical Abstract: The swine-origin 2009 pandemic H1N1 virus (pdmH1N1) is genetically related to North American swine H1 influenza viruses and unrelated to human seasonal H1 viruses. Currently, specific diagnosis of pdmH1N1 relies on RT-PCR. In order to develop an assay that does not rely in amplification of the viral genome, a conventional sandwich ELISA for detection of the pdmH1N1 was developed. The sandwich ELISA was based on three monoclonal antibodies (3B2, 5H7, and 12F3) against pdmH1N1. 5H7 and 12F3 were selected as capture antibodies and biotin-conjugated 3B2 was subsequently selected as the detection antibody in the ELISA. The results showed the ELISA had high specificity for pdmH1N1 strains and no reaction with other swine H1 viruses, human seasonal H1N1 or H3N2 viruses, or avian influenza viruses. The limit of detection of the ELISA ranged from 3.2 x 103 to 1.5 x 104 TCID50 /ml. When the ELISA was used to detect viruses in nasal wash samples from infected ferrets, it showed 90.1% sensitivity and 100% specificity compared to the "gold standard" - virus isolation. Our studies highlight a convenient assay for specific diagnosis of the 2009 pandemic H1N1-like viruses.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov