U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 close



Research
                Research Home
                            National Programs
                            Research Projects
                            Scientific Manuscripts
                            International Engagement
                            Scientific Software/Models
                            Databases and Datasets
                            Office of Scientific Quality Review
            Media
                News & Features
                            Multimedia
                            Briefing Room
                            Down on the Farm
                            Press Room
                            Science in Your Shopping Cart
                            Scientific Discoveries
                            Tellus
                            Under the Microscope
            About ARS
                About ARS Home
                            Staff Directory
                            Labs and Research Centers (Map)
                            Headquarter Offices
                            Organizational Chart
                            Employee Services (REE Employees Only)
                            Office of Outreach, Diversity, and Equal Opportunity
            Work With Us
                Work With Us Home
                            ARS Vacancies at USAJOBS
                            Careers at ARS Information
                            Scientific Collaborations
                            Extramural Agreements
                            1890 Faculty Research Sabbatical ProgramSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            SHIN, JOO-HEON - Johns Hopkins Universityitem
                                            Li, Robertitem
                                            GOA, YUAN - Johns Hopkins Universityitem
                                            Bickhart, Derekitem
                                            Liu, Ge

                                                         - *George*item
                                            LI, WEIZHIONG - University Of Californiaitem
                                            WU, SITAO - University Of Californiaitem
                                            Li, Congjun

                                                         - *Cj*
  Submitted to: PLoS Genetics
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 4/20/2012
                                    Publication  Date: 1/22/2013
                                    Citation: Shin, J., Li, R.W., Goa, Y., Bickhart, D.M., Liu, G., Li, W., Wu, S., Li, C. 2013. Butyrate induced IGF2 activation correlated with distinct chromatin landscapes due to histone modification. PLoS Genetics. DOI:10.4137/GRSB.S11243.Interpretive Summary: Insulin-like growth factor 2 (IGF2) is an important gene for animal growth. Its function depends on specific regulation to activate the gene.  The regulation mechanisms are unclear. We used state of the art molecular biology technologies to investigate the regulation mechanisms of this gene. Our results provide insights into the mechanisms of butyrate-induced activation of IGF2 and regulation of gene expression by histone modification.
                                Technical Abstract: Histone modification has emerged as a very important mechanism regulating the transcriptional status of the genome. Insulin-like growth factor 2 (IGF2) is a peptide hormone controlling various cellular processes such as proliferation and apoptosis.  IGF2 and H19 are reciprocally regulated imprinted genes.  Our previous studies demonstrate that butyrate regulates the expression of IGF2 as well as genes encoding IGF Binding proteins.  To obtain further understanding of histone modification and its regulatory potentials in controlling IGF2/H19 gene expression, we investigated the histone modification status of some key histone associated with the expression of IGF2/H19 genes using RNA-seq in combination with Chip-seq technology. A high-resolution map of the major chromatin modification at the IGF2/H19 locus induced by butyrate was constructed to illustrate the fundamental association of chromatin modification landscape that may play a role in the activation of IGF2 gene.  High definition epigenomic landscape mapping revealed that IGF2 and H19 have distinct chromatin modification patterns at their coding and promoter regions, such as TSSs and TTSs. Moreover, the correlation between the differentially methylated regions (DMRs) of IGF2/H19 locus and histone modification (acetylation and methylation) indicated that epigenetic signatures/markers of DNA methylation, histone methylation, and histone acetylation were differentially distributed on the expressed IGF2 and silenced H19 genes. Our evidence also suggests that butyrate induced regional changes of histone acetylation status in the up-stream regulation domain of H19 may be related to the reduced expression of H19 and strong activation of IGF2.  Our results provided insights into the mechanism of butyrate induced loss of imprinting (LOI) of IGF2 and regulation of gene expression by histone modification.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov