U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Cereal Crops Research: Fargo, ND
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    ETSARC Research Units
                        ETSARC Home page
                                    Office of the Center Director
                                    Location Support Staff
                                    Animal Metabolism-Agricultural Chemicals Research
                                    Cereal Crops Research
                                    Insect Genetics and Biochemistry Research
                                    Sugarbeet and Potato Research
                                    Sunflower and Plant Biology ResearchSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Plains Area Fargo, North Dakota Edward T. Schafer Agricultural Research Center Cereal Crops Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Xu, Stevenitem
                                            Niu, Zhixiaitem
                                            Klindworth, Darylitem
                                            ZHANG, QIJUN - North Dakota State Universityitem
                                            Chao, Shiaomanitem
                                            Friesen, Timothyitem
                                            Jin, Yueitem
                                            Rouse, Matthew

                                                         - *Matt*item
                                            Faris, Justinitem
                                            CAI, XIWEN - North Dakota State University
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 8/14/2013
                                    Publication  Date: 9/14/2013
                                    Citation: Xu, S.S., Niu, Z., Klindworth, D.L., Zhang, Q.J., Chao, S., Friesen, T.L., Jin, Y., Rouse, M.N., Faris, J.D., Cai, X. 2013. Chromosome engineering for alien gene introgression in wheat: Progress and prospective. Triticeae Cytogenetics: Past, Present and Future, September 15-16, 2013, Kyoto, Japan. p. 24-25.Interpretive Summary:
                                Technical Abstract: Chromosome engineering is a useful strategy for introgression of desirable genes from wild relatives into cultivated wheat.  However, it has been a challenge to transfer a small amount of alien chromatin containing the gene of interest from one genome to another non-homologous genome through classical chromosome engineering.  Only a small number of useful alien genes have been isolated from large alien chromosomal segments and successfully used in wheat breeding and production.  In an effort to utilize wild species-derived genes for controlling the newly-emerged Ug99 stem rust races, we initiated this research to reduce or eliminate linkage drag associated with four Ug99-resistance genes (Sr37 from Triticum timopheevii, Sr39 and Sr47 from Aegilops speltoides, and Sr43 from Thinopyrum ponticum) and to transfer novel Sr genes from several wheat-alien species (Ae. markgrafii, Th. intermedium, and Th. junceum) disomic addition lines into the wheat genome.  To minimize linkage drag, we utilized a new scheme of chromosome engineering to reduce alien chromatin surrounding the Sr genes.  In this procedure, we first developed a large homoeologous recombinant population using the ph1b mutant in hexaploid wheat and a Ph1-deficient cytogenetic stock in tetraploid wheat.  Homoeologous recombinants carrying the Sr genes on reduced alien chromosomal segments were identified using high-throughput phenotyping and genotyping, robust DNA markers, and genomic in situ hybridization.  By using this procedure, we developed wheat germplasm carrying the four Sr genes (Sr37, Sr39, Sr43, and Sr47) on small alien chromosomal segments.  To transfer novel Sr genes from the wheat-alien species addition lines, we used 'Chinese Spring' Ph1 inhibitor or monosomics to induce recombination between wheat chromosomes and their homoeologs carrying the Sr genes from alien species.  New wheat lines containing four novel Sr genes on translocated alien chromosome segments were developed from the crosses of the wheat-alien species addition lines with the Ph1 inhibitor or monosomics.  The new wheat lines developed in this study provide useful resources for the ongoing global breeding efforts to combat Ug99. We are currently applying these procedures to transfer other novel genes for resistance to stem rust, leaf rust, and Hessian fly from wild species into wheat.  

References

(1)	Klindworth DL, Niu Z, Chao S, Friesen TL, Jin Y, Faris JD, Cai X, Xu SS. Introgression and characterization of a goatgrass gene for a high level of resistance to Ug99 stem rust in tetraploid wheat, Genes, Genomes, Genetics 2:665-673 (2012).

(2)	Niu ZX, Klindworth DL, Friesen TL, Chao S, Jin Y, Cai X, Xu SS. Targeted introgression of a wheat stem rust resistance gene by DNA marker-assisted chromosome engineering. Genetics 187:1011–1021 (2011).

(3)	Xu SS, Jin Y, Klindworth DL, Wang RR-C, and Cai X. Evaluation and characterization of seedling resistance to stem rust Ug99 races in wheat-alien species derivatives. Crop Sci. 49:2167–2175 (2009).


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov