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Title:   Author  item
                                            Hughes, Stephenitem
                                            Moser, Bryanitem
                                            HARMSEN, AMANDA - Illinois State Universityitem
                                            Bischoff, Kennethitem
                                            JONES, MARJORIE - Illinois State Universityitem
                                            PINKLEMAN, REBECCA - South Dakota School Of Mines And Technologyitem
                                            BANG, SOOKIE - South Dakota School Of Mines And Technologyitem
                                            Doll, Kenneth

                                                         - *Ken*item
                                            TASAKI, KEN - Mitsubishi Chemical Usa, Incitem
                                            Qureshi, Nasibitem
                                            Liu, Siqingitem
                                            Saha, Badalitem
                                            JACKSON, JR, JOHN - Former ARS Employeeitem
                                            Cotta, Michaelitem
                                            Rich, Josephitem
                                            CAIMI, PAOLO - Mitsubishi Chemical Usa, Inc
  Submitted to: Symposium Proceedings
                                    Publication Type: Proceedings
                                    Publication Acceptance Date: 6/8/2011
                                    Publication  Date: 6/8/2011
                                    Citation: Hughes, S.R., Moser, B.R., Harmsen, A.J., Bischoff, K.M., Jones, M.A., Pinkleman, R., Bang, S.S., Doll, K.M., Tasaki, K., Qureshi, N., Liu, S., Saha, B.C., Jackson, Jr, J.S., Cotta, M.A., Rich, J.O., Caimi, P. 2011. High-throughput automated molecular biology platform for production of fuel ethanol yeast capable of expressing high-value heterologous proteins. Proceedings of the Seventh International Starch Technology Conference. p. 21-29.Interpretive Summary:
                                Technical Abstract: The majority of fuel ethanol currently produced in the United States is made from corn starch.  Projections indicate that corn supplies will not be able to meet the increasing demand for biofuels.  Lignocellulosic biomass, an abundant and renewable carbon source, has the potential to supplement starch feedstocks for the production of fuel ethanol, but current technology is constrained by production costs.  The profitability of ethanol production from lignocellulosic biomass will be improved if high-value co-products are also generated.  Current processes for fuel ethanol production from starch yield substantial amounts of corn oil as a byproduct.  Corn oil triacylglycerides are converted to fatty acid ethyl esters (biodiesel) and glycerol by transesterification with ethanol.  One method of catalyzing this transesterification reaction is with lipase enzymes.  An integrated biorefinery combining starch ethanol and cellulosic ethanol facilities may become cost-effective if biodiesel is produced as a co-product using lipase-catalyzed single-step column transesterification with low-cost lipases expressed in large quantities in a recombinant yeast strain capable of cellulosic and corn ethanol production.  We have engineered such a recombinant ethanologenic yeast strain to express a lipase that could be used for biodiesel production from the corn oil byproduct of the starch ethanol process in an integrated biorefinery.


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