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Title:   Author  item
                                            MUEHLBAUER, GARY - University Of Minnesotaitem
                                            BODDU, JAYANAND - University Of Minnesotaitem
                                            GARDINER, STEPHANIE - University Of Minnesotaitem
                                            SHIN, SANGHYUN - University Of Minnesotaitem
                                            JIA, HAIYAN - University Of Minnesotaitem
                                            CHO, SEUNGHO - University Of Minnesotaitem
                                            McCormick, Susanitem
                                            SCHWEIGER, WOLFGANG - University Of Natural Resources & Applied Life Sciences - Austriaitem
                                            LEMMONS, MARC - University Of Natural Resources & Applied Life Sciences - Austriaitem
                                            BERTHILLER, FRANZ - University Of Natural Resources & Applied Life Sciences - Austriaitem
                                            HAMETNER, CHRISTIAN - Vienna University Of Technologyitem
                                            KOVALSKY PARIS, PAULA - University Of Natural Resources & Applied Life Sciences - Austriaitem
                                            TORRES-ACOSTA, JUAN - University Of Natural Resources & Applied Life Sciences - Austriaitem
                                            ADAM, GERHARD - University Of Natural Resources & Applied Life Sciences - Austria
  Submitted to: American Phytopathological Society Abstracts
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 8/8/2012
                                    Publication  Date: 8/8/2012
                                    Citation: Muehlbauer, G.J., Boddu, J., Gardiner, S., Shin, S., Jia, H., Cho, S., Mccormick, S.P., Schweiger, W., Lemmons, M., Berthiller, F., Hametner, C., Kovalsky Paris, P.M., Torres-Acosta, J.A., Adam, G. 2012. The role of trichothecenes in the Triticeae-Fusarium graminearum interactions. American Phytopathological Society Abstracts.Interpretive Summary:
                                Technical Abstract: Fusarium Head Blight (FHB), caused by Fusarium graminearum, is a major disease problem for the small grain crops wheat and barley. During infection, F. graminearum produces trichothecene mycotoxins such as deoxynivalenol (DON) that increase the aggressiveness of the fungus and reduces grain quality. Thus, we are interested in identifying genes that protect wheat and barley from the toxic effects of trichothecenes. Previous work had identified an Arabidopsis DOGT1 gene, encoding an UDP-glucosyltransferase (UGT), as involved in trichothecene resistance via conjugation of DON to DON-3-glucoside (D3G). We examined transcript profiles in wheat and barley during Fusarium graminearum infection and inoculation with the trichothecene deoxynivalenol (DON). From these experiments, we identified a set of 10 barley UDP-glucosyltransferases (UGT) that were upregulated during F. graminearum infection or DON treatment. We screened this set of UGTs in yeast and identified a barley UGT (HvUGT13248) gene that exhibited resistance to DON via conjugation to D3G. Transgenic Arabidopsis overexpressing HvUGT13248 exhibited the ability to grow on media containing DON. DON feeding studies on the transgenic Arabidopsis showed that DON was conjugated to D3G. In contrast to prior work in Arabidopsis overexpressing DOGT1 exhibited a dwarf phenotype due to brassinosteroid conjugation, transgenic Arabidopsis overexpressing HvUGT13248 did not exhibit dramatic changes in morphology and did not conjugate the brassinosteroid castasterone. More recently, we developed transgenic wheat overexpressing HvUGT13248 and showed that these lines exhibited high type II FHB resistance.


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