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Title:   Author  item
                                            Lillehoj, Hyunitem
                                            JANG, SEUNG - Research Institute Of Health And Environmentitem
                                            Lee, Sungitem
                                            LEE, KYUNG - Animal, Plant And Fisheries Quarantine And Inspection Agency (QIA)item
                                            LILLEHOJ, ERIC - University Of Marylanditem
                                            HONG, YEONG - Chung-Ang Universityitem
                                            AN, DONG - Animal, Plant And Fisheries Quarantine And Inspection Agency (QIA)item
                                            JEONG, WOO - Animal, Plant And Fisheries Quarantine And Inspection Agency (QIA)item
                                            CHUN, JI - Animal, Plant And Fisheries Quarantine And Inspection Agency (QIA)item
                                            BERTRAND, FRANCOIS - Seppic, Incitem
                                            DUPUIS, LAURENT - Seppic, Incitem
                                            DEVILLE, SEBASTIEN - Seppic, Incitem
                                            BEN AROUS, JULIETTE - Seppic, Inc
  Submitted to: Vaccine
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 9/20/2012
                                    Publication  Date: 12/16/2012
                                    Citation: Lillehoj, H.S., Jang, S.I., Lee, S.H., Lee, K.W., Lillehoj, E.P., Hong, Y.H., An, D.J., Jeong, W.S., Chun, J.E., Bertrand, F., Dupuis, L., Deville, S., Ben Arous, J. 2012. Vaccination with Clostridium perfringens recombinant proteins in combination with MontanideTM ISA 71 VG adjuvant increases protection against experimental necrotic enteritis in commercial broiler chickens. Vaccine. 30(36):5401-5406.Interpretive Summary: Ability to boost immune response to simple proteins of enteric bacteria is needed to develop an effective recombinant vaccination strategy against some pathogens which are difficult to control using conventional live vaccines.  ARS scientists collaborated with scientists from Korea and France to show that a commercially available mineral oil-in-water adjuvant elicited host immune response to protein antigens when protein antigens were emulsified in this adjuvant.  The commercial MontanideTM ISA 71 VG adjuvants elicited serum antibodies against clostridium proteins that were used as immunogens and induced partial protective immune response when vaccinated birds were challenged with live Clostridium perfringens.  Based on immunological parameters that were evaluated in this study, this commercial ISA 71 VG mineral oil-in-water adjuvant showed immune enhancing effect in broiler chickens.  These results suggest that vaccination with certain clostridial proteins in combination with ISA 71 VG mineral oil adjuvant enhances mucosal immunity against experimental necrotic enteritis in broiler chickens.
                                Technical Abstract: This study was performed to compare four Clostridium perfringens recombinant proteins as vaccine candidates using the MontanideTM ISA 71 VG adjuvant in an experimental model of necrotic enteritis.  Broiler chickens were immunized with clostridial recombinant proteins with ISA 71 VG, and intestinal lesion scores, body weight gains, antibody levels, and proinflammatory cytokines were measured after oral co-infection with C. perfringens and Eimeria maxima.  Birds immunized with all recombinant proteins plus ISA 71 VG showed significantly reduced gut lesions compared with the ISA 71 VG-only group.  Birds immunized with NetB toxin or PFO plus ISA 71 VG exhibited significantly increased body weight gains compared with the ISA 71 VG alone group.  Greater NetB toxin antibody titers were observed in the NetB/ISA 71 VG group, and greater PFO antibody titers were evident in the PFO/ISA 71 VG group, each were compared with the other three vaccine/adjuvant groups.  Finally, decreased levels of gene transcripts encoding interleukin-8, tumor necrosis factor superfamily 15, and LPS-induced TNF-a factor were observed in the intestinal lymphocytes of chickens immunized with NetB toxin, PFO, a-toxin, and/or EF-Tu in the presence of ISA 71 VG compared with ISA 71 VG alone.  All parameters evaluated were equal in co-infected chickens given ISA 71 VG alone compared with infected/adjuvant-free birds, indicating that the adjuvant itself did not have a disease protective effect.  These results suggest that vaccination with clostridial recombinant proteins, particularly NetB toxin or PFO, in combination with ISA 71 VG enhances protective immunity against experimental necrotic enteritis in broiler chickens.


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