U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 close



Research
                Research Home
                            National Programs
                            Research Projects
                            Scientific Manuscripts
                            International Engagement
                            Scientific Software/Models
                            Databases and Datasets
                            Office of Scientific Quality Review
            Media
                News & Features
                            Multimedia
                            Briefing Room
                            Down on the Farm
                            Press Room
                            Science in Your Shopping Cart
                            Scientific Discoveries
                            Tellus
                            Under the Microscope
            About ARS
                About ARS Home
                            Staff Directory
                            Labs and Research Centers (Map)
                            Headquarter Offices
                            Organizational Chart
                            Employee Services (REE Employees Only)
                            Office of Outreach, Diversity, and Equal Opportunity
            Work With Us
                Work With Us Home
                            ARS Vacancies at USAJOBS
                            Careers at ARS Information
                            Scientific Collaborations
                            Extramural Agreements
                            1890 Faculty Research Sabbatical ProgramSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Jenkins, Markitem
                                            Obrien, Celiaitem
                                            Macarisin, Dumitruitem
                                            Karns, Jeffreyitem
                                            Santin-Duran, Monicaitem
                                            Fayer, Ronald
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 6/29/2010
                                    Publication  Date: 7/31/2010
                                    Citation: Jenkins, M.C., Obrien, C.N., Macarisin, D., Karns, J.S., Santin, M., Fayer, R. 2010. Characterization of giardin protein expression during encystation of Giardia duodenalis. Meeting Abstract.Interpretive Summary:
                                Technical Abstract: Giardia duodenalis trophozoites attach to the gut surface by means of a ventral disk that contains various giardin proteins that appear to be important to VD structural integrity. One approach to preventing giardiasis is to stimulate giardin-specific antibodies and thereby block trophozoite attachment to the gut epithelium.  Understanding giardin expression during encystation (cyst formation) or excystation (trophozoite release) might provide clues to the role of giardins in the Giardia life-cycle.  In the present study expression of major giardin proteins during in vitro conversion of trophozoites to cysts was characterized.  Quantitative RT-PCR using giardin-specific primers provided data on the expression of each respective gene sequence relative to house-keeping genes over time.  Immunoblotting using giardin-specific antisera revealed the presence of beta- and delta-giardin at all timepoints during encystation.  Moreover, immunofluorescence assay showed that both giardins were localized to a well-defined ventral disk early in encystation, and then was increasingly localized to an amorphous structure inside the cysts as early as 48 hr during encystation. Dual color labeling of 
G. duodenalis trophozoites with antisera to delta and beta giardin followed by confocal epifluorscence microscopy revealed the spatial organization of the two major giardin proteins in the trophozoite stage.  These findings confirm earlier observations that the VD is disassembled during encystation, and provide interesting insight on the unique metabolic activities of G. duodenalis during encystation.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov