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Title:   Author  item
                                            HEAD, KATIE - University Of Texas Southwestern Medical Centeritem
                                            GALOS, TIM - Purdue Universityitem
                                            FANG, YUANJIN - Purdue Universityitem
                                            Hudson, Karen
  Submitted to: Molecular Breeding
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 1/18/2012
                                    Publication  Date: 2/21/2012
                                    Citation: Head, K., Galos, T., Fang, Y., Hudson, K.A. 2012. Mutations in soybean KASIIa gene are correlated with high levels of seed palmitic acid. Molecular Breeding. doi:1007/s11032-012-9707-x.Interpretive Summary: Soybean is an important oilseed crop. Soybean oil is composed of several fatty acids, including palmitic acid, which is a 16-carbon saturated fatty acid. For some baking fats, it is desirable to have an oil with a high saturated fat content. For health reasons, it is desirable to reduce the amounts of saturated fats in soybean oil. To achieve a stable modification of the fatty acid content it is important to identify plants with genetic variation in palmitic acid content, and understand the function of enzymes involved in the process of fatty acid biosnythesis. The mutations described in this paper contribute to our understanding of the function of the enzyme required to convert palmitic acid to stearic acid, and can be used by breeders to modify the palmitic acid content of soybean seed.
                                Technical Abstract: A complete understanding of the biosynthetic pathways involved in the formation of soybean seed oils is required to develop lines with useful oil profiles. In particular, modification of the content of saturated fatty acids using genetics has been a target for soybean breeders for many years. One step in the fatty acid biosynthesis pathway in soybean embryos requires keto-acyl-ACP synthase IIa (KASIIa) for the conversion of palmitic acid to stearic acid. Progeny seeds from approximately 5000 NMU-mutagenized lines from the soybean TILLInG population were screened for fatty acid composition. In this screen a number of mutant soybean lines with a heritable increase in seed palmitic acid levels were identified. In a total of five mutagenized lines, candidate gene sequencing revealed potentially damaging single nucleotide changes in the KASIIa gene. In two of these lines, the defect is likely to affect mRNA splicing.  Missense mutations (A487T, D406N) were identified in two lines, and one line contained an early termination codon. These mutations represent new mutant alleles of KASIIa available in soybean germplasm. These new alleles may be useful to breeders in order to enhance seed oil content.


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