U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Crops Pathology and Genetics Research: Davis, CA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Outreach Events
                        UC Davis Picnic Day
                                    Kinders go to College
                                    STEM Girls
                                    Future Scientist Program
                                    Agriculture Future of America
                                    Wallace-Carver Fellowship
                                    Young Scholars Program
                                    Annual Awards Banquet
                    About Us
                        About Us
                                    Our Mission
                                    Principal InvestigatorsSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Pacific West Area Davis, California Crops Pathology and Genetics Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications
- Etiology and Biology of Tree Crop Diseases
- Rice Genetics
- Sustainable Floriculture
- Sustainable Viticulture

Title:   Author  item
                                            MEIR, SHIMON - Volcani Center (ARO)item
                                            Jiang, Cai-Zhongitem
                                            LERS, AMNON - Volcani Center (ARO)item
                                            PHILOSOPH-HADAS, SONIA - Volcani Center (ARO)item
                                            BURD, SHAUL - Volcani Center (ARO)item
                                            SUNDARESAN, SRIVIGNESH - Volcani Center (ARO)item
                                            VIJAY SELVARAJ, K.S. - Volcani Center (ARO)item
                                            KOCHANEK, BETTINA - Volcani Center (ARO)item
                                            MACNISH, ANDREW - University Of Californiaitem
                                            REID, MICHAEL - University Of California
  Submitted to: Symposium Proceedings
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 4/28/2009
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: Abscission, the separation of organs from the parent plant, results in postharvest quality loss in many ornamentals and other fresh produce. The process is initiated by changes in the auxin gradient across the abscission zone (AZ), is triggered by ethylene, and may be accelerated by postharvest stress.  Although changes in gene expression have been correlated with the ethylene-mediated execution of abscission, there is almost no information on the molecular and biochemical basis of the increase in sensitivity of the AZ to ethylene. We examined transcriptome changes in the tomato (Solanum lycopersicum Mill.) flower AZ during the rapid acquisition of ethylene sensitivity following flower removal, which depletes the AZ from auxin.  Microarray analysis using Affymetrix Tomato GeneChip revealed changes in expression, occurring prior to and during pedicel abscission, of many genes with possible regulatory functions.  They included a range of auxin- and ethylene-related transcription factors (TFs), other TFs that are transiently induced just after flower removal, and a set of novel AZ-specific genes.  To facilitate functional studies we implemented and efficient Virus-Induced Gene Silencing (VIGS) system in tomato using tobacco rattle virus (TRV) and an anthocyanin regulatory gene (Lc) as a silencing reporter.  A phenotype showing a significant delay in pedicel abscission in response to auxin depletion (obtained by flower removal) was observed when we silenced several novel AZ-specific genes. These results shed light on the mechanism of increased sensitivity of the AZ to ethylene, and further expand our knowledge of auxin-ethylene cross talk during the abscission process.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov