U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Obesity and Metabolism Research: Davis, CA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Laboratories
                        Bennett Group
                                    Huang Group
                                    Keim Group
                                    Laugero Group
                                    Newman GroupSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Pacific West Area Davis, California Western Human Nutrition Research Center Obesity and Metabolism Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            SCHIE, IWAN - University Of Californiaitem
                                            Pedersen, Theresaitem
                                            SMITH, ZACHARY - University Of Californiaitem
                                            WU, JIAN - University Of Californiaitem
                                            Newman, Johnitem
                                            HUSER, THOMAS - University Of Californiaitem
                                            NOLTEL, LENA - Bielefeld Universityitem
                                            YAHIATENE, IDIR - University Of California
  Submitted to: Analyst
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 8/15/2013
                                    Publication  Date: 8/15/2013
                                    Citation: Schie, I.W., Pedersen, T.L., Smith, Z., Wu, J., Newman, J.W., Huser, T., Noltel, L., Yahiatene, I. 2013. Direct comparison of fatty acid ratios in single cellular lipid droplets as determined by Raman spectroscopy and gas chromatography. Analyst. doi: 10.1039/C3AN00970J.Interpretive Summary: Lipid droplets inside cells are the least studied and least understood cellular organelles in eukaryotic and prokaryotic cells. Despite broad research efforts to understand the structure and function of lipid droplets it has previously not been possible to determine the composition of individual cellular lipid droplets. In this paper we present a novel data analysis approach called non-negative least-squares fitting to analyze a type of infra red spectra from single cellular lipid droplets to determine their composition after cellular exposure to different amounts of oleic and palmitic acid. By comparing the results from the single lipid droplet analysis to those from the gas chromatography mass spectrometry analysis of millions of cells, we show that the proposed method can accurately predict the relative amount of a fatty acid in the lipid droplet, and that the composition in a single lipid droplet is on average the same as that of all lipid droplets found in the sample.
                                Technical Abstract: Cellular lipid droplets are the least studied and least understood cellular organelles in eukaryotic and prokaryotic cells. Despite a broad research trying to understand lipid droplets it has not been possible to determine the composition of individual cellular lipid droplets. In this paper we present a novel approach based non-negative least-squares fitting of Raman spectra from single cellular lipid droplets to determine the composition of individual lipid droplets in cells after a treatment with different amounts of oleic and palmitic acid. By comparing the results from the single lipid droplet analysis to the results from gas chromatography analysis of millions of cells, we can show that proposed method can accurately predict the relative amount of a fatty acid in the lipid droplet, and that the composition in a single lipid droplet is on average the same as that of all lipid droplets found in the sample.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov