U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Virus and Prion Research: Ames, IA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area Ames, Iowa National Animal Disease Center Virus and Prion Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Brockmeier, Susanitem
                                            Nicholson, Tracyitem
                                            Loving, Crystalitem
                                            Register, Karen
  Submitted to: American Society for Microbiology General Meeting
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 2/28/2011
                                    Publication  Date: 5/21/2011
                                    Citation: Brockmeier, S., Nicholson, T.L., Loving, C.L., Register, K.B. 2011. Contribution of Bordetella bronchiseptica type III secretion system to respiratory disease in swine [abstract]. American Society for Microbiology 2011 Annual Meeting. Abstract No. 1494.Interpretive Summary:
                                Technical Abstract: Background: The type III secretion system (TTSS) of gram negative bacteria allows injection of effector proteins directly into the cytosol of eukaryotic cells. Previous studies have demonstrated that the B. bronchiseptica TTSS plays a role in the persistent bacterial colonization of the trachea of mice and rats by modulating host immune responses. We hypothesized that the TTSS would be necessary for B. bronchiseptica persistence in the swine respiratory tract. Methods: To investigate the role of the TTSS in Bordetella pathogenesis in swine, we constructed mutants containing an in-frame deletion of the bscN gene in KM22, a virulent B. bronchiseptica swine isolate. This gene encodes an essential component for TTSS secretion across bacterial membranes. We compared this mutant to KM22 for its ability to colonize and cause disease. Sixteen pigs each were inoculated intranasally with 10**6 CFU of either wild-type KM22, the TTSS mutant of KM22, or PBS as a control. Nasal colonization was determined by performing nasal washes on days 1, 3, 5, 7, and weekly thereafter through day 56 after inoculation. Tracheal and lung colonization was determined by euthanizing 4 pigs from each group on days 7, 14, 28 and 56 after inoculation. Results: Colonization of the TTSS mutant was similar to wild-type KM22 in the nasal cavity throughout the experiment. Colonization of the TTSS mutant was similar to wild-type KM22 in the trachea and the lung on day 7, but was significantly lower after that. In addition, fewer pigs (6/16) inoculated with the TTSS mutant developed pneumonic lesions than pigs inoculated with the wild-type KM22 (14/16). Conclusion: Our results indicate that the TTSS is required for optimal colonization in the lower respiratory tract and disease severity in swine.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov