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ARS Home Midwest Area Madison, Wisconsin U.S. Dairy Forage Research Center Cell Wall Biology and Utilization Research Research Publications at this Location





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Title:   Author  item
                                            Zeller, Wayneitem
                                            Powell, Josephitem
                                            HAGEN, TIMOTHY - Northern Illinois Universityitem
                                            HELGREN, TRAVIS - Northern Illinois Universityitem
                                            LINDQUIST, SARA - Northern Illinois University
  Submitted to: American Chemical Society Abstracts
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 12/20/2012
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: Inhibition of ammonia production in manure provides for a healthier environment for dairy farmers working in confined quarters. The resulting conservation of urea provides for an enhanced nitrogen concentration, and thus, fertilizing capacity, of the manure. The use of a mixture of tannins from quebracho (condensed) and chestnut (hydrolyzable) trees has been shown to reduce ammonia production in manure during simulated barn floor experiments. In an effort to determine the types of tannin structures responsible for this ammonia abatement activity, we examined a series of commercially available flavanoid monomers and hydrolyzable tannins for urease inhibition/ammonia abatement activity. In vitro IC50 determinations of these compounds against Jack bean urease showed that urease inhibitory activity follows epigallocatechin gallate > tannic acid > quercitin > pentagalloglucose > catechin. In ammonia emissions chambers, epigallocatechin gallate, at 0.28 and 0.56% by weight, afforded 50% and 76% reduction of ammonia emissions, respectively, from manure samples over a 48-h period.


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