U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Horticultural Crops Research Unit: Corvallis, OR
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Other
                        History
                                    Useful Links
                    HortHappenings News PageSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Pacific West Area Corvallis, Oregon Horticultural Crops Research Unit Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Lee, Jungminitem
                                            Rennaker, Christopher

                                                         - *Chris*
  Submitted to: Acta Horticulturae
                                    Publication Type: Proceedings
                                    Publication Acceptance Date: 6/23/2008
                                    Publication  Date: 3/2/2009
                                    Citation: Lee, J., Rennaker, C.D. 2009. Comparison of two methods for anthocyanin quantification. Acta Horticulturae. 810:831-834.Interpretive Summary: This study examined commonly used methods (HPLC and the pH differential methods) for determining the anthocyanin content of samples. Anthocyanins are the compounds that give small fruits and their processed products red, blue, and black colors. The two methods examined had a high correlation with one another. The pH differential method is a good alternative for labs that do not have access to an HPLC for quantifying anthocyanin content. Detailed findings from this study were presented at the conference.
                                Technical Abstract: The pH differential method (AOAC method 2005.02) by spectrophotometer, and high performance liquid chromatography (HPLC) are methods commonly used by researchers and the food industry for quantifying anthocyanins of samples or products. This study was carried out to establish a relationship between these two frequently utilized analytical methods. Seven juice samples, each containing an array of individual anthocyanins, were analyzed by pH differential and HPLC (two different conditions). Additional samples that had been processed in the lab were also included in the comparison (n > 180). This study demonstrated a high correlation (R > 0.925, p = 0.05) between the pH differential method and HPLC (both systems) when determining the amount of anthocyanins within samples. In both methods, total anthocyanins were greater when values were expressed as malvidin-3-glucoside than as cyanidin-3-glucoside. For laboratories that do not have the capability for HPLC analysis, the pH differential offers a straightforward and economical method to determine total anthocyanins. This study also demonstrated the importance of reporting the standard used to express the values. Methods and certified anthocyanin standards are critical for anthocyanin research and the food industry.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov