U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 close



Research
                Research Home
                            National Programs
                            Research Projects
                            Scientific Manuscripts
                            International Engagement
                            Scientific Software/Models
                            Databases and Datasets
                            Office of Scientific Quality Review
            Media
                News & Features
                            Multimedia
                            Briefing Room
                            Down on the Farm
                            Press Room
                            Science in Your Shopping Cart
                            Scientific Discoveries
                            Tellus
                            Under the Microscope
            About ARS
                About ARS Home
                            Staff Directory
                            Labs and Research Centers (Map)
                            Headquarter Offices
                            Organizational Chart
                            Employee Services (REE Employees Only)
                            Office of Outreach, Diversity, and Equal Opportunity
            Work With Us
                Work With Us Home
                            ARS Vacancies at USAJOBS
                            Careers at ARS Information
                            Scientific Collaborations
                            Extramural Agreements
                            1890 Faculty Research Sabbatical ProgramSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Northeast Area Wyndmoor, Pennsylvania Eastern Regional Research Center Food Safety and Intervention Technologies Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Gurtler, Joshuaitem
                                            Douds, Daviditem
                                            DIRKS, BRIAN - Drexel Universityitem
                                            QUINLAN, JENNIFER - Drexel Universityitem
                                            Niemira, Brendan
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 3/2/2011
                                    Publication  Date: 8/2/2011
                                    Citation: Gurtler, J., Douds, D.D., Dirks, B.P., Quinlan, J.J., Niemira, B.A. 2011. Influence of mycorrhizal fungi on survival of salmonella and E.coli O157:H7 in soil and translocation into allium porrum roots and stem. International Association for Food Protection Annual Meeting, Milwaukee, Wisconsin, July 31 - August 3, 2011., Volume 1, P. 1.Interpretive Summary:
                                Technical Abstract: Modern agriculture disrupts the natural symbiotic relationship arbuscular mycorrhizal (AM) fungi have with most vegetable plants, which may affect translocation of human pathogens into the plant.

Five-month-old Allium porrum (leek) plants (with or without AMF [Glomus intraradices]) were used as a model.  Plants were inoculated (8 ml per plant) with composite suspensions of either nalidixic acid resistant Salmonella or EHEC at a low level (5 log CFU/ml) or a high level (8 log).  Samples were taken one day following inoculation as well as weekly up to 1 and 3 weeks for low and high inocula, respectively.  Samples were plated on XLT4PN and tcSMACPN, respectively.  Pre-enrichments were made in TSBPN, while secondary enrichments were in TTPN for Salmonella and a novel TSBselect broth for EHEC.   

Both pathogens at the high inoculum level were detected at higher levels in AMF+ roots and shoots versus non-AMF roots through the three week sampling.  Pathogens were ca. 0.5 log CFU/g higher in the AMF-free soil versus AMF+ soil at week 3, suggesting possible AMF-mediated suppression.  Pathogens at the low inoculum level were detected by direct plating on day one, but only by enrichment for non-AMF Salmonella-inoculated roots at one week.  Both pathogens in the soil at the low inoculum level were ca. 4.4 log CFU/g on day one; however, at the one week sampling time, Salmonella levels were 3 log CFU/g, while EHEC levels were 2.2 log CFU/g, with or without AMF.  All negative controls tested negative for pathogens.

These results indicate that Allium porrum can be colonized in the root and shoot when soil is inoculated with either Salmonella or E. coli O157:H7.  Symbiotic AMF may reduce survival of human pathogens in the soil.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov