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ARS Home Midwest Area Ames, Iowa National Animal Disease Center Infectious Bacterial Diseases Research Research Publications at this Location





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Title:   Author  item
                                            SCHILLER, IRENE - Prionics Agitem
                                            SCHRODER, B - Prionics Agitem
                                            VORDERMEIER, H - Veterinary Laboratories Agency (VLA)item
                                            Waters, Wadeitem
                                            Palmer, Mitchellitem
                                            Thacker, Tyleritem
                                            WHELAN, ADAM - Veterinary Laboratories Agency (VLA)item
                                            HARDEGGER, ROLAND - Prionics Agitem
                                            MARG-HAUFE, BEATRICE - Prionics Agitem
                                            RAEBER, ALEX - Prionics Agitem
                                            OESCH, BRUNO - Prionics Ag
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 8/25/2009
                                    Publication  Date: 8/25/2009
                                    Citation: Schiller, I., Schroder, B., Vordermeier, H.M., Waters, W.R., Palmer, M.V., Thacker, T.C., Whelan, A., Hardegger, R., Marg-Haufe, B., Raeber, A., Oesch, B. 2009. Comparison of Tuberculin Activity in the Interferon-gamma Assay for the Diagnosis of Bovine Tuberculosis [abstract].Interpretive Summary:
                                Technical Abstract: Cattle infected with bovine tuberculosis still represent a serious regulatory and health concern in a variety of countries. Early diagnosis using the in vitro interferon gamma (IFN-gamma) assay has been applied for more than a decade. Briefly, IFN-gamma responses in whole blood cultures stimulated with purified protein derivatives (PPDs) from Mycobacterium bovis (PPD-B) or M. avium (PPD-A) are quantitated. The production of a higher amount of IFN-gamma in cultures stimulated with PPD-B in comparison to PPD-A is indicative of an M. bovis infection. In the present study, different sources and concentrations of PPDs were compared with regard to diagnostic reliability in experimentally- and naturally-infected animals. Significant differences (P < 0.05) between sources and concentrations of PPD for stimulation were detected, indicating the need for standardization of PPDs used in the IFN-gamma assay. Additionally, we propose a tool named Relative Potency 30 (RP30) that allows rapid comparison of batches and sources of PPDs.


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