U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Healthy Processed Foods Research: Albany, CA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Pacific West Area Albany, California Western Regional Research Center Healthy Processed Foods Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications
- Rebecca Milczarek *(Scopus)*
- Yuzhu Zhang *(PubMed)*

Title:   Author  item
                                            Bartley, Glennitem
                                            Yokoyama, Wallace

                                                         - *Wally*item
                                            YOUNG, SCOTT - Dow Chemical Companyitem
                                            ANDERSON, KERR - Dow Chemical Companyitem
                                            HUNG, SHAO-CHING - Dow Chemical Companyitem
                                            ALBERS, DAVID - Dow Chemical Companyitem
                                            LANGHORST, MARSHA - Dow Chemical Companyitem
                                            TUROWSKI, MACIEJ - Dow Chemical Companyitem
                                            Kim, Hyunsook
  Submitted to: Journal of Nutrition
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 4/10/2010
                                    Publication  Date: 7/1/2010
                                    Citation: Bartley, G.E., Yokoyama, W.H., Young, S.A., Anderson, K., Hung, S., Albers, D.R., Langhorst, M.L., Turowski, M., Kim, H. 2010. Hypocholesterolemic effects of hydroxypropyl methylcellulose are mediated by altered gene expression in hepatic bile and cholesterol pathways of male hamsters. Journal of Nutrition. 140(7): 1255-1260.Interpretive Summary: Recently combination of lifestyle changes and appropriately controlled diets has received more attention to reduce cardiovascular disease risk. Hydroxypropyl methylcellulose (HPMC) is a well-characterized semi-synthetic cellulose derivative that has the structural and physiological properties of a soluble non-fermentable dietary fiber. In the present study, HPMC supplemented diets showed significant effects of cholesterol and body weight reduction in hamsters. Furthermore, these dramatic changes were associated with hepatic lipid metabolism-related gene expression. Our data suggested that increased bile acid and sterol excretion with HPMC supplemented diets could trigger dramatic changes in lipid and cholesterol metabolism in liver. This study further supports the potential dietary use of HPMC for the prevention of cardiovascular disease and possibly obesity
                                Technical Abstract: Hydroxypropyl methylcellulose (HPMC), a semi-synthetic non-fermentable soluble dietary fiber (SDF) modulates plasma lipoprotein profiles and hepatic lipid levels. HPMC is not absorbed by the body but its presence in the intestinal lumen increases fecal fat, sterol, and bile acid excretion and decreases intestinal cholesterol absorption which may result indirectly in changes in hepatic lipid metabolism. To elucidate the underlying mechanism, we measured hepatic expression of genes involved in cholesterol, bile acid, and fatty acid metabolism in hamsters fed 39% fat calorie diets containing either 5% HPMC or 5% insoluble dietary fiber (microcrystalline cellulose, MCC) for 4 weeks. Hamsters fed HPMC gained significantly less body weight without changes in food intake and had significantly lower plasma total-, VLDL-, and LDL-cholesterol and triglyceride concentrations and hepatic lipid levels than animals on MCC diet. Cholesterol-lowering effects of HPMC supplementation were significantly correlated with up-regulation of the expression of hepatic CYP7A1, CYP51, and HMG-CoA reductase genes, indicating both increased cholesterol and bile acid synthesis. Interestingly, regulation of CYP7A1 expression was not dependent of FXR' expression but dependent of HNF-4'. Up-regulation of hepatic LDLR expression was inversely correlated with plasma cholesterol levels. Reductions in plasma and hepatic triglyceride levels were associated with hepatic expression of genes related to fatty acid oxidation (up-regulation) and fatty acid synthesis (down-regulation). Our data suggest that altered hepatic expression of lipid metabolism-related genes, possibly due to modulation of fecal bile acid excretion and intestinal cholesterol absorption, contributes to lipid-lowering effects of HPMC.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov