U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Cell Wall Biology and Utilization Research: Madison, WI
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area Madison, Wisconsin U.S. Dairy Forage Research Center Cell Wall Biology and Utilization Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Hall, Mary Beth
  Submitted to: Journal Dairy Science Supplement
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 2/25/2011
                                    Publication  Date: 7/10/2011
                                    Citation: Hall, M. 2011. Evaluation of specificity of hydrolysis methods for separation of water-soluble carbohydrates. Journal of Dairy Science. 94(E-Supplement 1):688.Interpretive Summary:
                                Technical Abstract: Various hydrolysis methods have been recommended to convert oligo- or polymeric water-soluble carbohydrates to monomers for detection in reducing sugar assays, but responses and specificity for different carbohydrates have not been well characterized. The study objective was to evaluate reducing sugar responses of sucrose (SUC), maltose (MAL), lactose (LAC), raffinose (RAF), and inulin (INU) to hydrolysis (HYD) by invertase, 0.5 M HCl, or 0.037 M H2SO4 or a mixture of sucrase/maltase/pullulanase/ß-amylase (Sucrase Mix). The p-hydroxybenzoic acid hydrazide reducing sugar method was used for detection. Hydrolyzed and unhydrolyzed samples were analyzed in 2 runs for each method. The statistical model included carbohydrate (WSC), HYD method, and the interaction (INT). Values presented were least squares means for reducing sugars as a percentage of sample DM. Unhydrolyzed carbohydrates differed in reducing sugar values (P<0.01; SUC = 1.1%, MAL = 54%, LAC = 42%, RAF = 2.0%, and INU = 7.4%, SED = 1.5%). LAC and MAL values reflect reactivity of the reducing end hexose in both molecules, whereas RAF and SUC lack reactive reducing end hexoses. The value for INU may reflect presence of a small amount of damaged molecules. Hydrolyzed minus unhydrolyzed values describe the amount of reducing sugar released by hydrolysis. This difference differed by WSC (P<0.01), HYD (P<0.01), and INT (P<0.01)(SED = 3.5%). LAC was largely unaffected by HYD. Because of complete or partial hydrolysis of SUC, RAF, and INU by the same treatments, and responses of unhydrolyzed LAC and MAL, it is concluded that these hydrolyses do not allow separation of specific WSC.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov