U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 close



Research
                Research Home
                            National Programs
                            Research Projects
                            Scientific Manuscripts
                            International Engagement
                            Scientific Software/Models
                            Databases and Datasets
                            Office of Scientific Quality Review
            Media
                News & Features
                            Multimedia
                            Briefing Room
                            Down on the Farm
                            Press Room
                            Science in Your Shopping Cart
                            Scientific Discoveries
                            Tellus
                            Under the Microscope
            About ARS
                About ARS Home
                            Staff Directory
                            Labs and Research Centers (Map)
                            Headquarter Offices
                            Organizational Chart
                            Employee Services (REE Employees Only)
                            Office of Outreach, Diversity, and Equal Opportunity
            Work With Us
                Work With Us Home
                            ARS Vacancies at USAJOBS
                            Careers at ARS Information
                            Scientific Collaborations
                            Extramural Agreements
                            1890 Faculty Research Sabbatical ProgramSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Lillehoj, Hyunitem
                                            LI, SHUHUI - Mississippi State Universityitem
                                            ZHANG, ZHENYU - Mississippi State Universityitem
                                            YAN, LIFANG - Mississippi State Universityitem
                                            PACE, LANNY - Mississippi State Universityitem
                                            ZHANG, SHUPING - Mississippi State University
  Submitted to: Avian Diseases
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 4/19/2010
                                    Publication  Date: 6/15/2010
                                    Citation: Lillehoj, H.S., Li, S., Zhang, Z., Yan, L., Pace, L., Zhang, S. 2010. Induction of CXC chemokine mRNA expression in chicken oviduct epithelial cells by Salmonella enterica serovar Enteritidis via the type three secretion system-1. Avian Diseases. 53(3):396-404.Interpretive Summary: Salmonella enterica serovar Enteritidis (SE) is an important pathogen for food safety and  has a broad range of hosts including mammals and poultry. Intestinal carrier-state is a significant source of horizontal transmission of Salmonella whereas reproductive tract colonization contributes to SE contamination of eggs and hatcheries. To better understand the protective immunity against Salmonella, ARS scientists and scientists at Mississippi State University collaborated  on the gene expression analysis of immune response genes following SE. One aspect of this study was on  the investigation of the role of  two virulence-associated type three secretion systems (T3SS-1 and T3SS-2) encoded by the Salmonella Pathogenicity Island-1 (SPI-1) and SPI-2, respectively since recent studies have demonstrated that T3SS-1 and T3SS-2 are required by Salmonella enterica to colonize chicken intestine and/or reproductive tissues. In this study, the messenger-RNA (mRNA) expression of cytokines in primary chicken oviduct epithelial cells (COEC) was determined using wild  type or type three secretion system (T3SS) mutant Salmonella enteritidis (SE) strains. All SE strains examined in this study elicited the expression of pro-inflammatory immune mediators including inducible nitric oxide synthase (iNOS), CXCLi1 (K60), CXCLi2 (IL-8), CCLi3 (K203), and CCLi4 (MIP-1ß). SE also triggered the expression of an anti-inflammatory cytokine, IL-10, but repressed TGF-ß3 transcription. T3SS-1 (sipA and sipB) mutants were significantly impaired in their ability to induce the expression of CXCLi1 and CXCLi2. T3SS-2 mutants displayed a wild type phenotype in terms of modulating the expression of chemokines and cytokines in COEC.  Genetic complementation of the sipA mutation restored a wild type phenotype indicating that SE-induction of CXCLi1 and CXCLi2 was sipA-dependent. These results will enhance our understanding of host-pathogen interaction in salmonellosis and will help to guide the future development of novel vaccines against salmonellosis.
                                Technical Abstract: The messenger-RNA (mRNA) expression of selected cytokines and chemokines in primary chicken oviduct epithelial cells (COEC) was determined following in vitro infection with wild type or type three secretion system (T3SS) mutant Salmonella enteritidis (SE) strains. All SE strains examined in this study elicited the expression of pro-inflammatory immune mediators including inducible nitric oxide synthase (iNOS), CXCLi1 (K60), CXCLi2 (IL-8), CCLi3 (K203), and CCLi4 (MIP-1ß). SE also triggered the expression of an anti-inflammatory cytokine, IL-10, but repressed TGF-ß3 transcription. Both T3SS-1 (sipA and sipB) and T3SS-2 (pipB and ssaV) mutants showed reduced capacity, compared to the wild type SE, to stimulate iNOS mRNA expression in COEC. T3SS-1 (sipA and sipB) mutants were significantly impaired in their ability to induce the expression of CXCLi1 and CXCLi2. T3SS-2 mutants displayed a wild type phenotype in terms of modulating the expression of chemokines and cytokines in COEC.  The expression of iNOS, but not CXC chemokines, correlated with the number of intracellular bacteria in COEC. Genetic complementation of the sipA mutation restored a wild type phenotype. Thus, SE-induction of CXCLi1 and CXCLi2 was sipA-dependent.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov