U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Dairy and Functional Foods Research: Wyndmoor, PA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    About ERRC
                        Greetings from the Director
                                    Directions/Lodging
                                    History and Accomplishments
                    Our Work
                        Biomass Pyrolysis Research
                                    IPMP 2013
                                    IPMP Dynamic Prediction
                                    IPMP Global Fit
                                    Pathogen Modeling Program (PMP)
                                    Predictive Microbiology Information Portal (PMIP)
                                    PoultryFARMSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Northeast Area Wyndmoor, Pennsylvania Eastern Regional Research Center Dairy and Functional Foods Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            BRITO, MARIA - Embrapaitem
                                            Somkuti, Georgeitem
                                            Renye, John
  Submitted to: Journal of Dairy Science
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 11/27/2010
                                    Publication  Date: 1/1/2011
                                    Citation: Brito, M.A., Somkuti, G.A., Renye Jr, J.A. 2011. Production of antilisterial bacteriocins by staphylococci isolated from bovine milk. Journal of Dairy Science. 94:1194-1200.Interpretive Summary: The control of staphylococci in dairy herds is important because these bacteria may cause infections and also outbreaks of food borne disease. However, some strains of staphylococci recovered from cattle produce natural antimicrobial products with high activity against bacteria causing milk-borne diseases such as Listeria and other staphylococci. Our survey of over 100 strains of staphylococci isolated from dairy herds in Brazil showed that close to 50 percent of cultures produced known or possibly novel antimicrobials that inhibited the growth of Listeria and staphyloccous cultures that cause udder infections in cows. The association of such staphylococci with dairy herds may be considered beneficial as they protect the health of animals by competitively inhibiting the growth of other infection causing staphylococci.  In some cases it may be possible to transfer this protective property to food grade bacteria that in turn may serve as natural biopreservatives of food systems controlling bacterial infections and leading to improved margins of food safety.
                                Technical Abstract: A collection of 111 staphylococcal isolates recovered from healthy cows in 41 dairy herds in Brazil was surveyed for the production of bacteriocins.  The group included 94 coagulase positive and 17 coagulase negative strains of staphylococci. All cultures were grown in tryptic soy broth for 18 h at 37 deg C and cell-free supernatants were tested for antimicrobial activity against several target organisms by the agar diffusion method. Filtrates of 57 staphylococci showed strong activity against Listeria monocytogenes Scott A and 52 isolates also inhibited the growth of Stapylococcus aureus Newbould 305, a major causative agent of bovine mastitis in the United States. The plasmid profiles of staphylococci invariably included an 8 kb plasmid. Staphylococcal isolates were tested for the production of aureocins A70 and A53, two bacteriocins of coagulase positive staphylococci known to be associated with 8 kb and 10.2 kb plasmids, respectively.  The presence of the A70 or A53 bacteriocin gene was checked by PCR techniques using primers based on nucleotide sequences flanking the structural gene of each bacteriocin. Agarose gel analysis of amplified PCR products of plasmid templates from all 58 isolates showed only a 525 bp fragment corresponding to the structural gene of the bacteriocin aureocin A70. The results indicated that the apparently widespread association of A70-producing staphylococci with healthy cows in Brazil may be beneficial in controlling undesirable bacteria in dairy herds.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov