U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 close



Research
                Research Home
                            National Programs
                            Research Projects
                            Scientific Manuscripts
                            International Engagement
                            Scientific Software/Models
                            Databases and Datasets
                            Office of Scientific Quality Review
            Media
                News & Features
                            Multimedia
                            Briefing Room
                            Down on the Farm
                            Press Room
                            Science in Your Shopping Cart
                            Scientific Discoveries
                            Tellus
                            Under the Microscope
            About ARS
                About ARS Home
                            Staff Directory
                            Labs and Research Centers (Map)
                            Headquarter Offices
                            Organizational Chart
                            Employee Services (REE Employees Only)
                            Office of Outreach, Diversity, and Equal Opportunity
            Work With Us
                Work With Us Home
                            ARS Vacancies at USAJOBS
                            Careers at ARS Information
                            Scientific Collaborations
                            Extramural Agreements
                            1890 Faculty Research Sabbatical ProgramSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Spackman, Ericaitem
                                            Suarez, Daviditem
                                            KILLIAN, MARY - Animal And Plant Health Inspection Service (APHIS)item
                                            KOSTER, LEO - Animal And Plant Health Inspection Service (APHIS)item
                                            SWENSON, SABRINA - Animal And Plant Health Inspection Service (APHIS)item
                                            PEDERSEN, JAN - Animal And Plant Health Inspection Service (APHIS)item
                                            Baker, Amyitem
                                            Lager, Kellyitem
                                            JENKINS-MOORE, MELINDA - Animal And Plant Health Inspection Service (APHIS)item
                                            SCHMITT, BEVERLY - Animal And Plant Health Inspection Service (APHIS)
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 4/16/2010
                                    Publication  Date: 4/18/2010
                                    Citation: Spackman, E., Suarez, D.L., Killian, M.L., Koster, L., Swenson, S., Pedersen, J., Vincent, A.L., Lager, K.M., Jenkins-Moore, M., Schmitt, B. 2010. Development and validation of a real-time RT-PCR assay for the 2009 pandemic H1N1 virus in veterinary specimens [abstract]. Swine Origin H1N1: The First Pandemic of the 21st Century meeting, April 18-20, 2010, Atlanta, Georgia. p. 30.Interpretive Summary:
                                Technical Abstract: Due to the transmissibility of the pH1N1 among different species including swine and turkeys, development of a differential diagnostic test for animal specimens was crucial for establishing surveillance and control programs.  The current diagnostic paradigm for veterinary specimens is to test for type A influenza, then to confirm positive samples with a lineage specific test.  To accomplish this the USDA type A influenza real-time reverse transcriptase-polymerase chain reaction (rRT-PCR) test, which targets the M gene, was updated to match the sequence of the pH1N1 M gene, and a new test which targets the neuraminidase (NA) gene was developed to specifically identify the 2009 pH1N1 lineage.  The modified M gene test achieved the highest sensitivity for the pH1N1 lineage although it can detect other type A influenza viruses.  The N1 test was successfully evaluated for specificity with a panel of 50 influenza viruses which included viruses of all 9 NA subtypes (18 isolates), 4 pH1N1 lineage viruses, 2 seasonal H1N1 lineage viruses, 14 North American H1N1 swine influenza virus (SIV) lineage viruses and 2 European Lineage H1N1 SIVs and 10 avian N1 subtype viruses.  The limit of detection was one 50% egg infectious doses per reaction.  Virus was detected in nasal swabs from experimentally infected pigs 1-5 days post exposure with the N1 test.  This assay has been implemented as an official test by the National Animal Health Lab Network.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov