U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 close



Research
                Research Home
                            National Programs
                            Research Projects
                            Scientific Manuscripts
                            International Engagement
                            Scientific Software/Models
                            Databases and Datasets
                            Office of Scientific Quality Review
            Media
                News & Features
                            Multimedia
                            Briefing Room
                            Down on the Farm
                            Press Room
                            Science in Your Shopping Cart
                            Scientific Discoveries
                            Tellus
                            Under the Microscope
            About ARS
                About ARS Home
                            Staff Directory
                            Labs and Research Centers (Map)
                            Headquarter Offices
                            Organizational Chart
                            Employee Services (REE Employees Only)
                            Office of Outreach, Diversity, and Equal Opportunity
            Work With Us
                Work With Us Home
                            ARS Vacancies at USAJOBS
                            Careers at ARS Information
                            Scientific Collaborations
                            Extramural Agreements
                            1890 Faculty Research Sabbatical ProgramSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Kim, Eui-Sooitem
                                            HONG, YEONG HO - Chung-Ang Universityitem
                                            Lillehoj, Hyun
  Submitted to: Poultry Science
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 8/15/2009
                                    Publication  Date: 2/1/2010
                                    Citation: Kim, E., Hong, Y., Lillehoj, H.S. 2010. Genetic effects analysis of MLF2 and TCR-B on resistance to coccidiosis in chickens. Poultry Science. 89:20-27.Interpretive Summary: Avian coccidiosis is caused by ubiquitous intestinal protozoan that significantly impair the growth and feed efficiency of infected chickens.  Although coccidiosis has been under control by coccidiostatic drugs, vaccination and genetic selection , emergence of coccidiosis resistant strains is decreasing the effectiveness of approaches.  Finding genes associated with coccidiosis resistance will be useful to efficient genetic selection for disease resistant lines and understanding mechanisms underlying disease resistance in chickens .  In this report, ARS scientists used QTL mapping studies to identify genes associated with disease resistance to avian coccidiosis, a parasitic disease caused by several distinct species of Eimeria. Resistance to coccidiosis can be considered as multigenic trait based on the variation of sickness after infection with Eimeria .  Using the methods of evaluating resistance or susceptibility to coccidiosis by multiple phenotypic measurements, the current study was conducted to identify SNPs that may play a role in the host response to coccidia infection defined by oocyst shedding. The current results showed that a statistically significant association between five SNPs in two genes (MLF2 and TCR-ß) and oocyst shedding which is major index for the resistance to avian coccidiosis. In addition to oocyst shedding, associations between SNPs in MLF2 and body weight support the relationship between MLF2 and coccidiosis resistance.  Single marker association analysis for 16 traits of coccidiosis resistance showed that a TCR-ß SNP and two MLF2 SNP were significantly associated with oocyst and body weights. Development of additional markers for candidate genes relevant to protective immunity will enhance feasibility of developing coccidiosis-resistance chickens.
                                Technical Abstract: Associations between parameters of resistance to coccidiosis and single nucleotide polymorphisms (SNPs) in three candidate genes located on chromosome 1 (TCR-ß, MLF2, and Lymphotactin) were determined.  SNPs were genotyped in 24 F1 generation and 290 F2 generation animals.  Four SNPs were identified in the Lymphotactin gene, 10 were located in the TCR-ß gene and 4 in the MLF2 gene.  At various times following experimental infection of the F2 generation with Eimeria maxima, body weights, fecal oocyst shedding, and biochemical parameters were measured as parameters of resistance.  Single marker association test was applied to determine the associations between the 20 SNPs and the parameters of coccidiosis resistance.  The maximum allele substitution effect on disease resistance of a SNP in MLF2 was explained by body weight (p = 0.0002).  The SNP in MLF2 significantly associated with body weight was also associated with both oocyst shedding (p = 0.001).  Four SNPs associated with oocyst shedding were found within coding region of TCR-ß.  While none of the Lymphotactin SNPs was associated with oocyst shedding, interferon-' level was associated with 2 SNPs in Lymphotactin.  These results suggest that MLF2 is a potential gene associated with resistance to experimental avian coccidiosis.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov