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Title:   Author  item
                                            Straus, David

                                                         - *Dave*item
                                            Farmer, Bradleyitem
                                            Beck, Benjamin
  Submitted to: Annual Eastern Fish Health Workshop
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 3/14/2011
                                    Publication  Date: 3/29/2011
                                    Citation: Straus, D.L., Farmer, B.D., Beck, B.H. 2011. Treating a natural outbreak of columnaris in channel catfish with copper sulfate and potassium permanganate [abstract]. 36th Annual Eastern Fish Health Workshop, March 28 – April 1, 2011, Mt. Pleasant , South Carolina. p.20.Interpretive Summary:
                                Technical Abstract: An F. Columnare-exclusive epizootic occurred in fingerling channel catfish (Ictalurus punctatus) during normal tank culture practices at SNARC.  Fish were transferred to the ultra low-flow system and 2.1 mg/L copper sulfate or 3 mg/L potassium permanganate was administered; an untreated control was also included, and there were 4 replications of each treatment.  The therapeutants were administered at 24 h intervals for 3 consecutive days.  The duration of the experiment was 7 d, and the survival on day 7 was 38.5%, 72.7% and 52.3% for the control, copper sulfate and potassium permanganate treatments, respectively.  Only copper sulfate was significantly different from the control (P < or = 0.05).  Twenty-four h after the last treatment, a fish from each replicate was sacrificed for gill histology and quantitative PCR analysis of the gill, caudal fin, and liver.  The gill, caudal fin, and liver of dead/moribund fish were cultured on selective Ordal’s agar to characterize the disease.  Further results will be presented for therapeutic dose-confirmation and quantitative PCR analysis of the water following each treatment.  This study suggests that copper sulfate was a more effective treatment than potassium permanganate.


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