U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Virus and Prion Research: Ames, IA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area Ames, Iowa National Animal Disease Center Virus and Prion Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Nicholson, Eric
  Submitted to: Journal of Veterinary Diagnostic Investigation
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 1/27/2011
                                    Publication  Date: 7/1/2011
                                    Citation: Nicholson, E.M. 2011. Enrichment of PrPSc in formalin-fixed, paraffin-embedded tissues prior to analysis by Western blot. Journal of Veterinary Diagnostic Investigation. 23(4):790-792.Interpretive Summary: This work reports an advance to a method to detect the prion protein, the causitve agent of transmissible spongiform encephalopathies (TSEs) such as bovine spongiform encephalopathy (BSE), scrapie, and chronic wasting disease (CWD). With this advance, the sensitivity of the detection is up to 5 times more sensitive than the previously published method, greatly enhancing the ability to use formalin-fixed paraffin embedded tissues for Western blot detection of TSEs.
                                Technical Abstract: Diagnosis of prion disease is primarily through immunodetection of the infectious agent. Typically, 2 distinct procedures are recommended for a definitive diagnosis with immunohistochemistry and Western blot providing the most information as to the specific isolate in question. In the past these approaches required formalin-fixed paraffin embedded tissue and fresh/frozen tissue respectively. This work describes an advance in methodology for Western blot analysis of formalin-fixed paraffin embedded tissues for the detection of PrP**Sc. With this modified procedure, 5 times the previously reported sample size may be used for analysis greatly enhancing the sensitivity of this procedure.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov