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Title:   Author  item
                                            Choi, Yoon-Eitem
                                            Goodwin, Stephen

                                                         - *Steve*
  Submitted to: Phytopathology
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 4/15/2010
                                    Publication  Date: 8/7/2010
                                    Citation: Choi, Y., Goodwin, S.B. 2010. Nutrient Acquisition and Generation of Reactive Oxygen Species Via CREA, AREA, and NOXa are Important in Pathogenicity in Mycosphaerella Graminicola. Phytopathology.Interpretive Summary:
                                Technical Abstract: Mycosphaerella graminicola is an important wheat pathogen causing significant economic loss. M. graminicola is a hemibiotroph, indicating that a biotrophic stage with nutrient uptake and a necrotrophic stage associated with a possible toxin or reactive oxygen species (ROS) are important to pathogenicity. To better understand the pathogenic mechanisms of M. graminicola, we employed over-expression strategies; selected target genes for over-expression were CREA, AREA, and NOXa, which might function as regulators in nutrient acquisition and ROS generation. Increased expressions of CREA, AREA, and NOXa were confirmed via q-RT PCR and subsequently used for pathogenicity testing. Among them, the NOXa over-expression strain, NO2, resulted in significantly increased pathogenicity. Moreover, instead of the usual filamentous growth, we observed a significant predominance of yeast-like growth in NO2, which is correlated with ROS production. Our data indicate that ROS generation via NOXa is important to pathogenicity as well as development in M. graminicola.


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