U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 close



Research
                Research Home
                            National Programs
                            Research Projects
                            Scientific Manuscripts
                            International Engagement
                            Scientific Software/Models
                            Databases and Datasets
                            Office of Scientific Quality Review
            Media
                News & Features
                            Multimedia
                            Briefing Room
                            Down on the Farm
                            Press Room
                            Science in Your Shopping Cart
                            Scientific Discoveries
                            Tellus
                            Under the Microscope
            About ARS
                About ARS Home
                            Staff Directory
                            Labs and Research Centers (Map)
                            Headquarter Offices
                            Organizational Chart
                            Employee Services (REE Employees Only)
                            Office of Outreach, Diversity, and Equal Opportunity
            Work With Us
                Work With Us Home
                            ARS Vacancies at USAJOBS
                            Careers at ARS Information
                            Scientific Collaborations
                            Extramural Agreements
                            1890 Faculty Research Sabbatical ProgramSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Yu, Qingzhongitem
                                            Estevez, Carlositem
                                            SONG, MINXUN - Shandong Academy Of Agricultural Sciencesitem
                                            Kapczynski, Darrellitem
                                            Zsak, Laszlo
  Submitted to: Virus Research
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 10/29/2009
                                    Publication  Date: 11/3/2009
                                    Citation: Yu, Q., Estevez, C., Song, M., Kapczynski, D.R., Zsak, L. 2010. Generation and biological assessment of recombinant avian metapneumovirus subgroup C (aMPV-C) viruses containing different length of the G gene. Virus Research. 147:182-188.Interpretive Summary: Avian metapneumovirus is an economically important pathogen of turkeys with a worldwide distribution. The glycoprotein (G) of the virus is believed to play an import role in initiating virus infection and stimulating host immune response. It has been shown that the length of the G gene varies among different avian metapneumovirus subtype C isolates. However, its biological significance in virus growth, virulence, and immunity is unknown. We developed a reverse genetics system for the virus and generated two Colorado (CO) strain-based recombinant viruses containing either the full-length G gene or a truncated G gene of the CO strain. The biological properties of these two recombinant G variants were assessed in Vero cells and in specific-pathogen-free turkeys. The results demonstrated that the large portion of the extracellular domain of the viral attachment protein is not essential for virus viability in cell cultures and in turkeys, but may play a role in enhancing virus attachment specificity and immunity in a natural host.
                                Technical Abstract: Genetic variation in length of the glycoprotein (G) gene among different avian metapneumovirus subgroup C (aMPV-C) isolates has been reported. However, its biological significance in virus replication, pathogenicity and immunity is unknown. In this study, we developed a reverse genetics system for aMPV-C and generated two Colorado (CO) strain-based recombinant viruses containing either the full-length G gene derived from a Canadian goose isolate or a C-terminally truncated G gene of the CO strain. The truncated short G (sG) gene encoded 252 amino acids (aa), which is 333 aa smaller than the full-length G (585 aa). The biological properties of these two recombinant G variants were assessed in Vero cells and in specific pathogen free (SPF) turkeys. In Vero cells, the short G variant displayed a similar level of growth dynamics and virus titers as the parental aMPV-CO strain, whereas the full-length G variant grew slower than the sG variant during the first 72 hours post-infection. Both of the G variants induced typical cytopathic effects (CPE) that were indistinguishable from those seen with the parental aMPV-CO infection.  In SPF turkeys, both of the G variants attenuated and caused little or no disease signs, but the full-length G variant appeared more readily grown in tracheal tissue than the sG variant during the first 5 days post-infection. Both G variants were immunogenic and induced a slightly different level of antibody responses. These results demonstrated that the large portion (333 aa) of the extracellular domain of the viral attachment protein is not essential for virus viability in vitro and in vivo, but may play a role in enhancing virus attachment specificity and immunity in a natural host.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov