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ARS Home Midwest Area Ames, Iowa National Animal Disease Center Infectious Bacterial Diseases Research Research Publications at this Location





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Title:   Author  item
                                            SCHRODER, B - Prionics Agitem
                                            HARDEGGER, R - Prionics Agitem
                                            MARG-HAUFE, B - Prionics Agitem
                                            SCHILLER, I - Prionics Agitem
                                            MOYEN, J - Department Of Agriculture Analysis Laboratoryitem
                                            Waters, Wadeitem
                                            Palmer, Mitchellitem
                                            Thacker, Tyleritem
                                            STAMM, C - Prionics Agitem
                                            Nonnecke, Brianitem
                                            KECK, N - Department Of Veterinary Laboratory Of L'Heraultitem
                                            EGNUNI, T - Veterinary Laboratories Agency (VLA)item
                                            WHELAN, A - Veterinary Laboratories Agency (VLA)item
                                            SIGAFOOSE, T - Animal And Plant Health Inspection Service (APHIS)item
                                            WELSH, M - Agri-Food And Biosciences Instituteitem
                                            CAGIOLA, M - Zooprofilattico Institute Of Umbria And Marchitem
                                            GORDON, S - University College - Irelanditem
                                            MEIJLIS, J - Lelystad Biologicalsitem
                                            CARTER, C - Asurequalityitem
                                            VORDERMEIER, H - Veterinary Laboratories Agency (VLA)item
                                            RAEBER, A - Prionics Ag
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 8/25/2009
                                    Publication  Date: 8/25/2009
                                    Citation: Schroder, B., Hardegger, R., Marg-Haufe, B., Schiller, I., Moyen, J.L., Waters, W.R., Palmer, M.V., Thacker, T.C., Stamm, C., Nonnecke, B.J., Keck, N., Egnuni, T., Whelan, A., Sigafoose, T., Welsh, M., Cagiola, M., Gordon, S., Meijlis, J., Carter, C., Vordermeier, H.M., Raeber, A.J. 2009. Improved Performance of the Interferon Gamma (IFN-gamma) Assay with Alternative Antigens for Stimulation of Whole Blood [abstract].Interpretive Summary:
                                Technical Abstract: 

Bovine tuberculosis caused by infection with Mycobacterium bovis (M. bovis) is a zoonotic disease that affects cattle worldwide and has a significant economic impact on the livestock industry. Although the disease has been effectively eliminated in many countries and regions, it is increasingly reappearing as a result of host reservoirs in wild animal populations. The success of bovine tuberculosis eradication programs in many countries have relied on ante mortem diagnostic tests measuring cell-mediated immune (CMI) responses such as the tuberculin skin test or the interferon gamma test. The BOVIGAM® interferon gamma (IFN-gamma) assay constitutes a laboratory-based tuberculosis test and is widely used complementary to the tuberculin skin test. The assay consists of a first step culturing whole blood with antigens and stimulating leucocytes to produce IFN-gamma which is quantified by ELISA in a second step. Diagnosis is based on a comparative measurement with avian and bovine tuberculins. Numerous field trials showed that the IFN-gamma assay was more sensitive than the intradermal skin test for diagnosis of tuberculosis. However, specificity of the IFN-gamma assay was found to be slightly lower than the skin test suggesting limited use of the IFN-gamma assay as a stand-alone screening tool in cattle herds that are either free or have a low prevalence of tuberculosis. In order to increase the specificity of the IFN-gamma test, defined antigens such as ESAT-6 or CFP-10 in combination with other highly specific antigens from the genome of M. bovis have been evaluated in field trials. Here we show, that a combination of antigens including ESAT-6 and CFP-10 showed a specificity of 99.5 % - 100 % in cattle from TB-free regions whereas sensitivities assessed in herds with skin test reactors in France and Ireland showed comparable sensitivities as with tuberculins. These results suggest that IFN-gamma testing comprising stimulation with well characterized antigen cocktails can be used as a stand alone test system for tuberculosis in cattle.


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