U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Livestock Issues Research: Lubbock, TX
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    NewsletterSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Plains Area Lubbock, Texas Cropping Systems Research Laboratory Livestock Issues Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            BURDICK, NICOLE - Texas Agrilife Researchitem
                                            AGADO, BRIAN - Texas Agrilife Researchitem
                                            RANDEL, RON - Texas Agrilife Researchitem
                                            NEUENDORFF, DON - Texas Agrilife Researchitem
                                            Carroll, Jeffery

                                                         - *Jeff Carroll*item
                                            VANN, RHONDA - Mississippi State Universityitem
                                            Chitko-Mckown, Carolitem
                                            LAWHON, SARA - Texas A&M Universityitem
                                            WELSH JR, TOM - Texas Agrilife Research
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 1/7/2010
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: Cortisol suppresses many aspects of immune function. However, recent publications suggest acute cortisol exposure may actually enhance immune function (Dhabhar. 2009. Neuroimmunomod. 16:300). The objective of this study was to determine the influence of acute increases in endogenous cortisol on expression of cytokines and the glucocorticoid receptor (GR) in isolated peripheral blood mononuclear cells (PBMCs). Blood samples were collected from Brahman heifers via jugular catheters at -3, 0, 1, 2, and 4 hours relative to a challenge with 0.1 IU/kg BW ACTH. Plasma cortisol was determined by RIA. The PBMCs were isolated and frozen until RNA isolation. Extracted RNA was amplified by RT-qPCR to determine expression of GR, tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), interleukin-4 (IL-4), and IL-10. Cortisol concentrations tended to decrease between cannulation (-3 hr) and initiation of the ACTH challenge (time 0 hr; P=0.07). Expression of IFN-gamma increased 16-fold relative to expression at cannulation. In response to ACTH, cortisol concentrations peaked at 1 hour (P less than or equal to 0.001) before decreasing to pre-challenge values. Expression of all cytokines and GR increased in response to ACTH, yet showed different expression patterns (P=0.01-0.06). This suggests that stimuli that increase endogenous cortisol concentrations may influence the expression of cytokines, and therefore modulate the immune system.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov