U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Virus and Prion Research: Ames, IA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area Ames, Iowa National Animal Disease Center Virus and Prion Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            CIACCI ZANELLA, JANICE - Labex - Embrapaitem
                                            Lager, Kellyitem
                                            Baker, Amyitem
                                            ZANELLA, ERALDO - Universidad De Passo Fundoitem
                                            GAVA, DANIELLE - Federal University Of Rio Grande Do Sul
  Submitted to: International Pig Veterinary Society (IPVS)
                                    Publication Type: Proceedings
                                    Publication Acceptance Date: 3/30/2010
                                    Publication  Date: 7/18/2010
                                    Citation: Ciacci Zanella, J.R., Lager, K.M., Vincent, A.L., Zanella, E.L., Gava, D. 2010. Challenge of Pigs with Natural Immunity to H1 and H3 Swine Influenza Virus with Pandemic 2009 H1N1 Influenza Virus. In: Proceedings of the International Pig Veterinary Society Congress, July 18-21, 2010, Vancouver, Canada. p. 253.Interpretive Summary:
                                Technical Abstract: Introduction. The emergence of the pandemic 2009 human H1N1 influenza A virus raised many questions about the implications for this virus in swine (1). One such question is, does prior exposure to influenza virus confer any protection against the new virus? This report describes a study to evaluate if natural exposure to swine influenza viruses confers protection against challenge with 2009 H1N1 virus.
  Materials and methods. Conventionally raised pigs were purchased from a sow herd negative for swine influenza A virus. Due to purchasing and shipping logistics, the pigs had been weaned and transported to a nursery facility that was geographically separated from the sow farm. Within 7 days of arrival at NADC most of the pigs were seropositive by the Avian Influenza MultiS-Screen ELISA (2) and virus was detected in the nasal swabs from a few pigs. Multiplex RT-PCR assays using four specific primers for H1 and H3, and N1 and N2 were performed (3). Twenty group 1 (G1) pigs were housed for 5 additional weeks (until approximately 10 weeks of age) at which time they were challenged with 2 x 10**6 TCID50 A/California/04/2009 H1N1 (CA09) by the intranasal route. Naive-age-matched pigs (n=4 pigs) or group 2 (G2) were also inoculated with CA09. Nasal swabs were taken on 0, 3 and 5 days post infection (dpi) to evaluate nasal shedding. Postmortem samples including serum, bronchoalveolar lavage fluid (BALF), lung, trachea and nasal swabs were collected at necropsy at day 5 pi. Serologic assays included hemagglutination inhibition (HI) assay (4) and ELISA (2). HI assays were used to evaluate 0, 3 and 5 dpi serum samples against CA09 (H1N1), OH07 (H1N1), MN99 (H1N1), and TX98 (H3N2) viruses. Real-time PCR assays were performed for quantitation of nucleic acids in BALF of pandemic H1N1 influenza virus (5), and for PRRSV, PCV2 and Mycoplasma hyopneumoniae using in house assays (6).
  Results and discussion. All pigs were negative for PRRSV, PCV2 and M. hyopneumoniae nucleic acids in BALF at necropsy. Genetic analysis of viruses isolated from nasal swabs and BALF samples from G1 pigs revealed the presence of simultaneous H1 and H3 virus infections prior to the start of the study at 5 weeks of age. After challenge with CA09, all naive-challenged pigs (G2) had macroscopic lung lesions with an average of 2.7%. The multifocal lesions appeared typical with well demarcated margins, purple in color. Nine of the 20 G1 pigs had detectable macroscopic lung lesions, but decreased in severity compared to the non-immune controls, with an average of 0.9%. The character of the G1 lung lesions was different compared to G2. The macroscopic lesions were purple-grey in color; involving the tips of the apical and cardiac lobes with less distinct margins. G1 pigs had no detectable virus titers in the lung or nasal swabs (0, 3 and 5 dpi). However, G2 pigs had CA09 viral titers in the lungs and nasal swabs with average titers of 10**1.6 TCID50/ml (3 dpi) and 10**2.3 TCID50/ml (5 dpi) in nasal swab samples, and of 10**3.7 TCID50/ml for BALF samples at 5 dpi. Real-time PCR for H1N1 pandemic virus in BALF (5 dpi) showed that 14/20 naturally immune pigs (G1) and 4/4 naive-challenged (G2) were positive for viral RNA. Quantitatively G2 samples presented an average of 9 x 10**6 copies of viral RNA / microliter of template. In contrast, G1 had an average of 1.4 x 10**3 copies, indicating a low viral replication in the lungs or residual RNA from challenge, confirming the negative results of virus isolation from nasal swabs and BALF. HI titers using the challenge virus (CA09) were not significant for either group at 0 and 5 dpi. However, significant HI titers against the gamma-cluster SIV OH07 were detected in G1 pigs at 0 dpi. H3N2 HI antibodies against TX98 were not detected in either group of pigs. These results indicate that pigs with natural immunity against gamma-cluster S


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov