U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Crops Pathology and Genetics Research: Davis, CA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Outreach Events
                        UC Davis Picnic Day
                                    Kinders go to College
                                    STEM Girls
                                    Future Scientist Program
                                    Agriculture Future of America
                                    Wallace-Carver Fellowship
                                    Young Scholars Program
                                    Annual Awards Banquet
                    About Us
                        About Us
                                    Our Mission
                                    Principal InvestigatorsSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Pacific West Area Davis, California Crops Pathology and Genetics Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications
- Etiology and Biology of Tree Crop Diseases
- Rice Genetics
- Sustainable Floriculture
- Sustainable Viticulture

Title:   Author  item
                                            Kasuga, Takaoitem
                                            GLASS, NANCY - University Of California
  Submitted to: Eukaryotic Cell
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 7/21/2008
                                    Publication  Date: 9/1/2008
                                    Citation: Kasuga, T., Glass, N.L. 2008. DISSECTING COLONY DEVELOPMENT OF NEUROSPORA CRASSA USING mRNA PROFILING AND COMPARTATIVE GENOMICS APPROACHES. Eukaryotic Cell. Vol. 7, No. 9, pp1549-1564.Interpretive Summary: A colony of fungus is comprised of long, branching filamentous cells called hyphae.  Genetic mechanisms underlying the development of hyphae are poorly understood.  We sectioned hyphae of a model fungus, Neurospora crassa (pink bread mold), into six parts depending on the age of the cells; 1 hour, 3 hour, 9 hour, 15 hour, 21 hour and 27 hour old, respectively, and analyzed functions of genes active in each of the sections.  We found that genes involved in cell membrane biosynthesis, polar growth and cellular signaling were active in the 1 hour section.  Genes involved in protein synthesis and energy production were active in the 9 and 15 hours old sections.  In the older sections (21 and 27 hours old) genes involved in protein degradation were found to be active.
                                Technical Abstract: Colony development, which includes hyphal extension, branching, anastomosis and asexual sporulation are fundamental aspects of the lifecycle of filamentous fungi; genetic mechanisms underlying these phenomena are poorly understood.  We conducted transcriptional profiling during colony development of the model filamentous fungus, Neurospora crassa, using 70-mer oligonucleotide microarrays.  Relative mRNA expression levels were determined for six sections of defined age excised from a 27 hr old N. crassa colony.  Functional category analysis showed that the expression of genes involved in cell membrane biosynthesis, polar growth and cellular signaling were enriched at the periphery of the colony.  The relative expression level of genes involved in protein synthesis and energy production were enriched in the middle section of the colony, while sections of the colony undergoing asexual development (conidiogenesis) were enriched in expression level for genes involved in protein/peptide degradation and unclassified proteins.  A cross-examination of the N. crassa dataset with a published dataset of Aspergillus niger revealed shared patterns in the spatiotemporal regulation of gene orthologs during colony development.  At present less than 50% of genes in N. crassa have functional annotation, which imposes the chief limitation on data analysis.  Using an evolutionary approach, we observed that the expression of phylogenetically conserved groups of genes was enriched in the middle section of a N. crassa colony, whereas expression of genes unique to euascomycete species as well as N. crassa-orphan genes was enriched at the colony periphery and in the older, conidiating sections of a fungal colony.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov