U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Livestock Issues Research: Lubbock, TX
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    NewsletterSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Plains Area Lubbock, Texas Cropping Systems Research Laboratory Livestock Issues Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            BURDICK, NICOLE - Texas Agrilife Researchitem
                                            AGADO, BRIAN - Texas Agrilife Researchitem
                                            RANDEL, RON - Texas Agrilife Researchitem
                                            NEUENDORFF, DON - Texas Agrilife Researchitem
                                            Carroll, Jeffery

                                                         - *Jeff Carroll*item
                                            VANN, RHONDA - Mississippi State Universityitem
                                            Chitko-Mckown, Carolitem
                                            LAWHON, SARA - Texas A&M Universityitem
                                            WELSH, JR., TOM - Texas Agrilife Research
  Submitted to: Journal of Animal Science Supplement
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 11/5/2009
                                    Publication  Date: 8/25/2010
                                    Citation: Burdick, N., Agado, B., Randel, R., Neuendorff, D., Carroll, J.A., Vann, R., Chitko Mckown, C.G., Lawhon, S., Welsh, Jr., T. 2010. Endogenous Cortisol: Acute Modulation of Cytokine Gene Expression in Bovine PBMCs [abstract]. Journal of Animal Science. 88:20(E-Suppl 3). Abstract #61.Interpretive Summary:
                                Technical Abstract: Cortisol suppresses many aspects of immune function. However, recent publications suggest acute cortisol exposure may actually enhance immune function (Dhabhar, Neuroimmunomod 2009;16:300). The objective of this study was to determine the influence of acute increases in endogenous cortisol on expression of cytokines and the glucocorticoid receptor (GR) in isolated peripheral blood mononuclear cells (PBMCs). Brahman heifers (n=12; 334±12 kg BW) had jugular catheters inserted prior to a challenge with 0.1 IU/kg BW ACTH. Blood samples were collected into EDTA vacutainers at -3, 0, 1, 2, and 4 hr relative to the challenge. Plasma cortisol was determined by RIA. PBMCs were isolated via density gradient centrifugation and frozen at -80oC until RNA isolation. Extracted RNA was amplified by real-time RT-PCR to determine expression of GR, tumor necrosis factor-alpha (TNF), interferon-gamma (IFN), interleukin-4 (IL-4), and IL-10. Cytokine expression data are expressed as the fold change in gene expression relative to samples collected at cannulation or time 0. All data were analyzed using the Mixed procedure of SAS, with time and animal as class and random variables, respectively. There was a tendency for cortisol concentrations to decrease between cannulation (-3 hr) and initiation of the ACTH challenge (time 0 hr; 26.2±4.4 and 16.1±4.4 ng/mL, respectively; P=0.07). Expression of most genes only tended to increase (P=0.06-0.14), with the exception of IFN (P=0.05), which increased 16-fold relative to expression at cannulation. In response to ACTH, cortisol concentrations peaked at 1 hr (52.4±2.77 ng/mL; P<0.01) before decreasing to pre-challenge values. Expression of IL-10 followed a similar pattern, with the greatest fold increase in expression at 1 hr (3.9±0.7 fold; P=0.02). Expression of GR, IL-4, and TNF increased through 4 hr post-challenge (9-92 fold; P=0.01-0.04). Expression of IFN tended to increase in response to ACTH challenge (28-fold; P=0.06). This suggests that stimuli that increase endogenous cortisol concentrations may influence the expression of cytokines, and therefore modulate the immune system.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov