U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Obesity and Metabolism Research: Davis, CA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Laboratories
                        Bennett Group
                                    Huang Group
                                    Keim Group
                                    Laugero Group
                                    Newman GroupSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Pacific West Area Davis, California Western Human Nutrition Research Center Obesity and Metabolism Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            TEPAAMORNDECH, SURAPUN - University Of Californiaitem
                                            Huang, Lipingitem
                                            Kirschke, Catherine
  Submitted to: Cancer Letters
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 4/14/2011
                                    Publication  Date: 5/28/2011
                                    Citation: Tepaamorndech, S., Huang, L., Kirschke, C.P. 2011. A null-mutation in the Znt7 gene accelerates prostate tumor formation in a transgenic adenocarcinoma mouse prostate model. Cancer Letters. 308 (2011) 33-42.Interpretive Summary: The prostate is a gland of the male reproductive system. The main function of the prostate is to store and secrete a clear fluid that helps prolong the lifespan of sperms. Decrease of zinc in the fluid secreting cells (epithelial cells) of human prostate has been implicated in the development of prostate cancer. To investigate the possibility that a decrease in zinc accumulation in the prostate epithelial cells has a role in prostate cancer development, we have generated and characterized a transgenic adenocarcinoma mouse prostate (TRAMP) model with a Znt7-null genetic background. Four-week-old male TRAMP mice (TRAMP/Znt7+/+ and TRAMP/Znt7-/-) were fed a semi-purified diet containing 15 mg Zn/kg diet for 2-24 weeks. Mice were euthanized at 6, 8, 16, and 24 weeks old. At 16 weeks of age, 25% TRAMP/Znt7-/- mice developed prostate tumors and two-third of mice with prostate tumors had tumor metastasized to the adjacent lymph nodes while no prostate tumor was detected in the control TRAMP mice. By 28 weeks, 67% TRAMP/Znt7-/- mice developed prostate tumors while only 22% control TRAMP mice developed prostate tumors. Furthermore, apoptosis (programmed cell death) was significantly reduced in the prostate of TRAMP/Znt7-/- mice. In conclusion, a null mutation of Znt7 gene promotes prostate cancer development in TRAMP mice.
                                Technical Abstract: Decrease of cellular zinc in the epithelium of the prostate has been implicated in the development of prostate cancer. To investigate whether ZnT7, a zinc transporter involved in intracellular zinc accumulation, plays a role in prostate cancer development, we have generated and characterized a transgenic adenocarcinoma mouse prostate (TRAMP) model with a Znt7-null genetic background. Four-week-old male TRAMP mice (TRAMP/Znt7+/+ and TRAMP/Znt7-/-) were fed a semi-purified diet containing 15 mg Zn/kg diet for 2-24 weeks. Mice were euthanized at 6, 8, 16, and 24 weeks for histopathological analysis of the prostates and for the presence of prostate tumors and lymph node metastasis. At 16 weeks of age, 25% TRAMP/Znt7-/- mice developed prostate tumors and two-third of mice with prostate tumors had tumor metastasized to the draining lymph nodes while no prostate tumor was detected in the control TRAMP mice. By 28 weeks, 67% TRAMP/Znt7-/- mice developed prostate tumors while only 22% control TRAMP mice developed prostate tumors. Furthermore, apoptosis was significantly reduced in the prostate of TRAMP/Znt7-/- mice. In conclusion, a null mutation of Znt7 gene promotes prostate cancer development in TRAMP mice.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov