U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 close



Research
                Research Home
                            National Programs
                            Research Projects
                            Scientific Manuscripts
                            International Engagement
                            Scientific Software/Models
                            Databases and Datasets
                            Office of Scientific Quality Review
            Media
                News & Features
                            Multimedia
                            Briefing Room
                            Down on the Farm
                            Press Room
                            Science in Your Shopping Cart
                            Scientific Discoveries
                            Tellus
                            Under the Microscope
            About ARS
                About ARS Home
                            Staff Directory
                            Labs and Research Centers (Map)
                            Headquarter Offices
                            Organizational Chart
                            Employee Services (REE Employees Only)
                            Office of Outreach, Diversity, and Equal Opportunity
            Work With Us
                Work With Us Home
                            ARS Vacancies at USAJOBS
                            Careers at ARS Information
                            Scientific Collaborations
                            Extramural Agreements
                            1890 Faculty Research Sabbatical ProgramSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Lillehoj, Hyunitem
                                            PARK, DONG WOON - VIS SCI, APDL, ARS, USDAitem
                                            LEE, SUNG HYEN - POST-DOC, APDL, ARS, USDAitem
                                            HAN, JAE YONG - SEOUL NAT UNIV, KOREAitem
                                            SHIN, JI HYE - ABII, GYEONGGI-DO, KOREAitem
                                            PARK, MYEUNG SUN - CHANGWON NAT UNIV, KOREAitem
                                            KIM, JIN-KYOO - CHANGWON NAT UNIV, KOREA
  Submitted to: Veterinary Immunology and Immunopathology
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 8/1/2008
                                    Publication  Date: 9/2/2008
                                    Citation: Hong, Y.H., Lillehoj, H.S., Park, D., Lee, S., Han, J., Shin, J., Park, M., Kim, J. 2008. Cloning and functional characterization of chicken interleukin-17D. Veterinary Immunology and Immunopathology.  126(1-2):1-8.Interpretive Summary: With the completion of human genome sequences and the availability of public genomic databases, a number of homologous proteins comprising an important member of immune genes have been described in poultry.  IL-17 family genes including IL-17A , -17B, -17C, -17D, -17E and -17F, as well as their cognate receptors, have no sequence similarity to any other known cytokines or receptors and thus appears to represent a distinct ligand-receptor signaling system and not much is known about these proteins in chickens. Among IL-17d families, IL-17D gene appeared to be most homologous to IL-17B and was preferentially expressed in resting CD4+ T cells, skeletal muscle, brain, pancreas, heart, lung and adipose tissue.  IL-17 family members have been linked to many disease processes such as rheumatoid arthritis, pulmonary disease, psoriasis, and allograft rejection. ARS scientists previously cloned IL-17A and showed this cytokine is important in coccidiosis. In this study, ARS scientists collaborated with scientists in Korea and cloned chicken IL-17d from a chicken testis cDNA library. The proinflammatory cytokine IL-17D was cloned from a testis cDNA library prepared from the Korean native chicken.  Comparison of the deduced amino acid sequence of chIL-17D with homologous proteins from human, mouse and opossum revealed 64%, 53% and 76%  and chIL-17D gene transcript was expressed in a wide range of tissues, and highest levels were in pancreas, thymus and lung.  Following Eimeria maxima infection, levels of the chIL-17D mRNA were up-regulated in the intestinal jejunum, bursa, lung, and spleen but decreased in the thymus, indicating their role in gut immunity. These results   demonstrate for the first time that chicken IL-17D protein has structural and functional similarities to mammalian IL-17Ds and that this cytokine plays an important role in local gut innate immune responses during experimental coccidiosis.
                                Technical Abstract: The proinflammatory cytokine IL-17D was cloned from a testis cDNA library prepared from the Korean native chicken.  The full-length chicken IL-17D (chIL-17D) cDNA consisted of a 348 nucleotide sequence encoding an open reading frame of 116 amino acids with a predicted molecular mass of 17.0 kDa.  Comparison of the deduced amino acid sequence of chIL-17D with homologous proteins from human, mouse and opossum revealed 64%, 53% and 76% identity respectively, including six conserved cysteine residues present in the mammalian polypeptides.  The chIL-17D gene transcript was expressed in a wide range of tissues, and highest levels were in pancreas, thymus and lung.  Following Eimeria maxima infection, levels of the chIL-17D mRNA were up-regulated in the intestinal jejunum, bursa, lung, and spleen but decreased in the thymus.  Infected chickens also expressed greater levels of chIL-17D mRNA in CD4+, CD8+ and TCR1+ intestinal intraepithelial lymphocytes while decreased expression was seen in TCR2+ cells.  Treatment of CHCC-OU2 fibroblasts with chIL-17D recombinant protein induced the expression of IL-6 and IL-8.  Collectively, these results suggest that chL-17D has structural and functional similarities to mammalian IL-17Ds and that it plays an important role in local gut innate immune responses during experimental coccidiosis.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov