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Title:   Author  item
                                            SCHNEIDERMAN, D - Agriculture And Agri-Food Canadaitem
                                            McCormick, Susanitem
                                            Alexander, Nancyitem
                                            JOHNSTON, A - Agriculture And Agri-Food Canadaitem
                                            HARRIS, L - Agriculture And Agri-Food Canada
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 11/4/2009
                                    Publication  Date: 11/4/2009
                                    Citation: Schneiderman, D., Mccormick, S.P., Alexander, N.J., Johnston, A., Harris, L.J. 2009. Disruption of Genes Involved in Butenolide and Culmorin Synthesis in Fusarium graminearum [abstract].Interpretive Summary:
                                Technical Abstract: Butenolide (4-acetamide-4-hydroxy-2-butenoic acid '-lactone) and culmorin (a tricyclic sesquiterpene diol) are two less-studied mycotoxins produced by several Fusarium species, including Fusarium graminearum.  A putative butenolide biosynthetic eight-gene cluster in F. graminearum includes fg08080 which encodes a zinc-finger protein and may function as a regulatory gene for the cluster.  Gene disruption of fg08080 resulted in loss of butenolide biosynthesis and the down-regulation of other genes within the cluster.  Fusarium infection in wheat revealed no difference in virulence between the fg08080-disrupted and wild-type strains.  fg08080 is required for butenolide biosynthesis in F. graminearum and may control butenolide biosynthesis through regulation of the gene cluster.

A terpene synthase gene, fg10397, was observed to be induced under trichothecene-inducing conditions and during plant infection (based on EST library representation, Northern and microarray analysis).  Transformed yeast cultures expressing FG10397 produced longiborneol, a terpene with the same tricyclic structure as culmorin, which was not produced by the progenitor yeast strain.  The fg10397 gene was disrupted in F. graminearum strain 9F1, a wild-type strain that produces a significant amount of culmorin in vitro.  No culmorin was produced by 9F1 strains with a disrupted fg10397 while wild-type and fg10397 add-back strains produced culmorin in liquid cultures.  fg10397 encodes a longiborneol synthase that is required for culmorin synthesis.


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