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Title:   Author  item
                                            TECHEN, NATASCHA - UNIV. OF MISSISSIPPIitem
                                            Arias De Ares, Reneeitem
                                            Glynn, Neilitem
                                            Pan, Zhiqiang

                                                         - *Peter*item
                                            KHAN, IKHLAS - UNIV. OF MISSISSIPPIitem
                                            Scheffler, Brian
  Submitted to: Molecular Ecology Resources
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 10/23/2009
                                    Publication  Date: 5/15/2010
                                    Citation: Techen, N., Arias De Ares, R.S., Glynn, N.C., Pan, Z., Khan, I.A., Scheffler, B.E. 2010. Optimized Construction of SSR-enriched Libraries. Molecular Ecology Resources. 10(3)508-515Interpretive Summary: Certain regions of the DNA in organisms are variable between individuals and these regions can be used as DNA markers to characterize variability within species or closely related species.  There are techniques to extract and identify those regions, though these methods are not very effective and are difficult to use.  We have designed a highly effective method to pull those variable regions of DNA for analysis, and we tested it on various types of DNA from different organisms, and showed its effectiveness on DNA samples of animals, plants and fungi.
                                Technical Abstract: We have developed a more efficient method to construct simple sequence repeat (SSR) libraries.  We have designed and tested several new adapter.  We changed the typical blunt-end ligation of adapters for the more effective sticky-end ligation.  We optimized temperature, enzymes and length of each step, and tested the system for making libraries of a nematode, a tree, a crop and a fungus to show that the system is effective using DNA of various origins.  We provide an easy to follow protocol for the users, and emphasize critical point to be successful in the constructions. The result was libraries containing SSRs on 74% or higher percentage of the contigs assembled.


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