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Title:   Author  item
                                            Hughes, Stephenitem
                                            Moser, Bryanitem
                                            HARMSEN, AMANDA - Illinois State Universityitem
                                            ROBINSON, SAMANTHA - Illinois State Universityitem
                                            Bischoff, Kennethitem
                                            JONES, MARJORIE - Illinois State Universityitem
                                            PINKELMAN, REBECCA - South Dakota School Of Mines And Technologyitem
                                            BANG, SOOKIE - South Dakota School Of Mines And Technologyitem
                                            TASAKI, KEN - Mitsubishi Chemical Usa, Incitem
                                            Doll, Kenneth

                                                         - *Ken*item
                                            Qureshi, Nasibitem
                                            Liu, Siqingitem
                                            Saha, Badalitem
                                            Jackson Jr, Johnitem
                                            Cotta, Michaelitem
                                            Rich, Josephitem
                                            CAIMI, PAOLO - Mitsubishi Chemical Usa, Inc
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 4/28/2010
                                    Publication  Date: 4/28/2010
                                    Citation: Hughes, S.R., Moser, B.R., Harmsen, A.J., Robinson, S., Bischoff, K.M., Jones, M.A., Pinkelman, R., Bang, S.B., Tasaki, K., Doll, K.M., Qureshi, N., Liu, S., Saha, B.C., Jackson Jr, J.S., Cotta, M.A., Rich, J.O., Caimi, P. 2010. Production of truncated Candida antarctica Lipase B gene using automated PCR gene assembly protocol and expression in yeast for use in ethanol and biodiesel production [abstract]. Advanced Biofuels Leadership Conference. Talk 3. p. 5.Interpretive Summary:
                                Technical Abstract: An improved column-based process for production of biodiesel was developed using a column containing a strongly basic anion-exchange resin in sequence with a column containing a resin to which a lipase biocatalyst is bound.  Currently most biodiesel is produced by transesterification of triglycerides, such as corn oil, with short-chain alcohols, such as methanol or ethanol, with a strong base as a catalyst.  The transesterification reaction to produce biodiesel can also be accomplished using resin-bound lipases as biocatalysts.  In this study, two columns were prepared, one containing the strongly basic anion-exchange resin DIAION® and the other containing Candida antartica lipase B enzyme bound to Sepabead® EC-EP resin.  These columns individually both catalyzed the transesterification of a mixture of ethanol and corn oil, producing comparable yields of ethyl esters (biodiesel).  When run in sequence, it was demonstrated that the yield of ethyl esters increased by 20%.


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