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ARS Home Northeast Area Geneva, New York Plant Genetic Resources Unit (PGRU) Research Publications at this Location





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Title:   Author  item
                                            WAN, YIZHEN - Northwest Agricultural & Forestry Universityitem
                                            Fazio, Gennaro
  Submitted to: Acta Horticulturae
                                    Publication Type: Proceedings
                                    Publication Acceptance Date: 12/15/2010
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: The Geneva® apple rootstock breeding program has made extensive use of Malus robusta cv. Robusta 5 as a source of resistance to fire blight.  Robusta 5 has also been used as the source of powdery mildew resistance by other breeding programs and a single locus Pl1 has been associated with this resistance and recently mapped on LG12 of the European Apple Framework Map [Plant Breeding 126:476-481, 2007] using a qualitative approach.  We collected quantitative data on field powdery mildew resistance in 2006 and 2007 on a segregating population made up of 186 individuals from a cross between Ottawa 3 (a susceptible apple rootstock) and Robusta 5; the correlation between the two years was 0.84 (p=0.000).  This quantitative data were used in combination with a molecular marker linkage map derived from the same cross to scan the genome using interval and MQM mapping in MapQTL 5 software.  The result was the identification of a large QTL on the distal end of LG12 with a peak near the SSR marker CH01b12z.  No other regions in the apple genome had any significant effects on resistance in this population when using Interval Mapping procedure.  MQM mapping revealed a .  Future plans include scanning of a BAC library containing Pl1 and utilizing existing markers for marker assisted breeding.


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