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Title:   Author  item
                                            Hughes, Stephenitem
                                            TAKAKI, KEN - Mitsubishi Chemical Usa, Incitem
                                            Moser, Bryanitem
                                            Doll, Kenneth

                                                         - *Ken*item
                                            BUTT, TAUSEEF - Lifesensors, Incitem
                                            STERNER, DAVID - Lifesensors, Incitem
                                            Bischoff, Kennethitem
                                            Hector, Ronald

                                                         - *Ron*item
                                            JONES, MARGE - Illinois State Universityitem
                                            BANG, SOOKIE - South Dakota School Of Mines And Technology
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 4/21/2009
                                    Publication  Date: 4/21/2009
                                    Citation: Hughes, S.R., Tasaki, K., Moser, B., Doll, K., Butt, T., Sterner, D., Bischoff, K., Hector, R., Jones, M., and Bang, S. 2009. GMAX yeast background strain made from industrial tolerant Saccharomyces cerevisiae engineered to convert pretreated Lignocellulosic starch and cellulosic sugars universally to ethanol anaerobically [abstract]. Cambridge Healthtech Institute, Advanced Biofuels Development Summit. Abstract No. 2. p. 3.Interpretive Summary:
                                Technical Abstract: Tailored GMAX yeast background strain technology for universal ethanol production industrially: Production of the stable baseline glucose, mannose, arabinose, xylose-utilizing (GMAX) yeast will be evaluated by taking the genes identified in high-throughput screening for a plasmid-based yeast to utilize xylose and glucose anaerobically for ethanol production.  The use of the xylose isomerase gene from Piromyces in combination with a modified xylulokinase gene from bacteria plus the anaerobic growth genes from S. cerevisiae allowed anaerobic growth on xylose and glucose simultaneously for cellulosic ethanol production and to be the background strain for other co-product gene expressions such as a lipase or an insecticidal genes.  The resulting stable transformed plasmids into any industrial yeast strains of Saccharomyces cervisiae that are already tolerant to environments in the production biorefinery are being developed for universal ethanol production from any feedstock provided.  Initially a cellulosic strain will be produced with XI, XKS, and one or more of the anerobic xylose utilization genes for use on acid or base hydrolysates.


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