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Title:   Author  item
                                            KANG, S - Pennsylvania State Universityitem
                                            MANSFIELD, M - Pennsylvania State Universityitem
                                            PARK, B - Pennsylvania State Universityitem
                                            GEISER, D - Pennsylvania State Universityitem
                                            IVORS, K - North Carolina State Universityitem
                                            COFFEY, M - University Of Californiaitem
                                            Grunwald, Niklaus

                                                         - *Nik*item
                                            Martin, Frankitem
                                            LEVESQUE, C - Agri Food - Canadaitem
                                            BLAIR, J - Franklin And Marshall College
  Submitted to: Phytopathology
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 4/10/2010
                                    Publication  Date: 8/2/2010
                                    Citation: Kang, S., Mansfield, M., Park, B., Geiser, D., Coffey, M., Grunwald, N.J. 2010. The promise and pitfalls of sequence-based identification of plant pathogenic fungi and oomycetes. Phytopathology. 100: 732-737.Interpretive Summary: Identification of plant pathogens remains a challenging task. Traditionally fungal microorganisms were identified using microscopic techniques. Contemporary analysis typically relies on analysis of selected genetic DNA sequences. Although DNA based approaches offer several advantages over traditional culture-based methods for pathogen diagnosis and identification, they have their own shortcomings. Here, we outline the potential benefits and drawbacks of using publicly accessible sequence databases for identification of plant pathogenic fungi and fungus-like organisms,using Phytophthora as a primary example.  We also discuss potential improvements for these pitfalls and the question of why coordinated community efforts are essential to making such remedies more efficient and robust.
                                Technical Abstract: Sequences of selected marker loci have been widely used for the identification of specific pathogens and the development of sequence-based diagnostic methods. Although such approaches offer several advantages over traditional culture-based methods for pathogen diagnosis and identification, they have their own shortcomings. Here, we outline the potential benefits and drawbacks of using publicly accessible sequence databases for identification and taxonomic deductions of plant pathogenic fungi and fungus-like organisms,using Phytophthora as a primary example; we also discuss potential remedies for these pitfalls and the question of why coordinated community efforts are essential to making such remedies more efficient and robust.


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