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ARS Home Plains Area Lincoln, Nebraska Wheat, Sorghum and Forage Research Research Publications at this Location





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Title:   Author  item
                                            Sattler, Scottitem
                                            KIM, RYAN - National Center For Genome Resourcesitem
                                            MUDGE, JOANN - National Center For Genome Resourcesitem
                                            FARMER, ANDREW - National Center For Genome Resourcesitem
                                            Funnell-Harris, Deannaitem
                                            Pedersen, Jeffrey
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 11/2/2009
                                    Publication  Date: 1/9/2010
                                    Citation: Sattler, S.E., Kim, R.W., Mudge, J., Farmer, A.D., Funnell-Harris, D.L., Pedersen, J.F. 2010. Global Expression in Sorghum Brown Midrib Mutants to Improve Biomass for Biofuels. Meeting Abstract. Plant & Animal Genome Conference XVIII, San Diego, CA Jan. 9-14, 2010.Interpretive Summary:
                                Technical Abstract: Brown midrib (bmr) mutants are being investigated for their ability to increase the conversion efficiency of sorghum biomass for lignocellulosic bioenergy.  Brown midrib 6 and 12 (bmr6 and 12) are impaired the last two steps of monolignol biosynthesis resulting in reduced lignin content and altered lignin composition.  Bmr6 and Bmr12 encode a cinnamyl alcohol dehydrogenase (CAD) and a caffeic acid-O-methyltransferase (COMT), respectively.  To comprehensively characterize the effects of bmr6 and bmr12 have on sorghum gene expression, the Illumina platform was used to sequence nearly 200 million reads representing reverse-transcribed mRNAs from bmr and wild-type stalks.  Expression profiles were generated from wild-type and near-isogenic lines containing bmr6, bmr12 or both bmr mutants in three different sorghum varieties.  A database of SNPs in expressed transcripts between the three sorghum varieties profiled Atlas, R Tx430 and Wheatland compared to JGI sequenced reference genome R Tx623 was generated. Differential expressed genes were identified in bmr mutants relative to wild-type, which may connect specific gene products functions in cell wall formation, lignification or bioenergy availability.  Roughly triple the number of genes were differentially expressed in bmr6 stalks relative to wild-type as compared to bmr12 relative to wild-type.  Together these data demonstrate that bmr6 and bmr12 each had distinct impacts upon gene expression, which may have specific plant physiological implications.


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