U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Western Human Nutrition Research Center: Davis, CA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Laboratories
                        Allen Group
                                    Bennett Group
                                    Stephensen Group
                                    Human Studies
                                    Metabolic Kitchen
                                    Huang Group
                                    Kable Group
                                    Keim Group
                                    Laugero Group
                                    Newman Group
                                    Lemay Group
                                    Support Staff
                    About Us
                        HistorySearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Pacific West Area Davis, California Western Human Nutrition Research Center Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications
- Charles Stephensen
- Charles Stephensen
- Brian Bennett
- Ellen Bonnel
- Yuriko Adkins
- Lindsay Allen
- Bess Caswell
- Liping Huang
- Mary Kable
- Nancy Keim
- Kevin Laugero
- Danielle Lemay
- John Newman
- Ryan Snodgrass
- Zeynep Alkan
- Allyson Deimeke
- Rashel (Shelly) DeCant
- Nicholas Esparza
- Steven Nguyen
- Erik Gertz
- Daniela Hampel
- Shurong Huang
- Xiaowen Jiang
- James Gomez
- Setti Shahab-Ferdows
- Debra Standridge
- Dave Storms

Title:   Author  item
                                            GERTOW, KARL - Karolinska Instituteitem
                                            NOBILI, ELENA - University Of Milanitem
                                            FOLKERSEN, LASSE - Karolinska Instituteitem
                                            Newman, Johnitem
                                            Pedersen, Theresaitem
                                            SWEDENBORG, JESPER - Karolinska Instituteitem
                                            PAULSSON-BERNE, GABRIELLE - Karolinska Instituteitem
                                            KUHN, HARTMUT - Charite' University Hospital Berlinitem
                                            HEDIN, ULF - Karolinska Instituteitem
                                            WHEELOCK, CRAIG - Karolinska Instituteitem
                                            HANSSON, GORAN - Karolinska Instituteitem
                                            HAEGGSTROM, JESPER - Karolinska Instituteitem
                                            GABRIELSEN, ANDERS - Karolinska Institute
  Submitted to: Atherosclerosis
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 1/11/2011
                                    Publication  Date: 1/21/2011
                                    Citation: Gertow, K., Nobili, E., Folkersen, L., Newman, J.W., Pedersen, T.L., Swedenborg, J., Paulsson-Berne, G., Kuhn, H., Hedin, U., Wheelock, C.E., Hansson, G.K., Haeggstrom, J.Z., Gabrielsen, A. 2011. 12- and 15-lipoxygenases in human carotid atherosclerotic lesions: Associations with cerebrovascular symptoms. Atherosclerosis. 215:411-416.Interpretive Summary: Lipoxygenase (ALOX) enzymes are implicated in both pro- and anti-atherogenic processes.  The aim of this study was to investigate five ALOX genes (ALOX12, ALOX12B, ALOX15, ALOX15B and ALOXE3) in human carotid artery atherosclerotic lesions (plaques), in relation to cerebrovascular symptoms and risk factors.  Specifically, levels of ALOX messenger RNAs (mRNA; the material facilitating the translation of genetic instructions into functional proteins) were investigated in plaque samples from the Biobank of Karolinska Endarterectomies (BiKE). BiKE consists of carotid plaque tissue specimens from patients undergoing surgical treatment of severe stenosis (narrowing) of the carotid artery and associated clinical data. Lesion mRNA levels were analyzed in a total of 132 BiKE samples. These analyses indicated that ALOX15B (also known as 15-LOX-2 or epidermis-type 15-LOX) is the only abundantly expressed mRNA in plaque tissue out of the five ALOX genes in question.  Correlations between ALOX15B mRNA levels and clinical parameters were investigated. Comparing plaques with or without attributable cerebrovascular symptoms (such as stroke or less severe, more transient symptoms), ALOX15B mRNA levels were found to be higher in plaques from symptomatic than asymptomatic subjects (1.31 [1.11-1.56], n=102; and 0.79 [0.55-1.15], n=30, respectively; p=0.008; mean [95%CI], arbitrary units). Protein and metabolite measurements in a limited number of lesions confirmed the presence of ALOX15B protein and products in plaque tissue.  In summary, we observed associations between high ALOX15B expression in carotid lesions and cerebrovascular symptoms. Although not allowing conclusions on causal relationships, these findings suggest a link between ALOX15B and atherothrombotic events that merits further investigation.
                                Technical Abstract: Lipoxygenase (ALOX) enzymes are implicated in both pro- and anti-atherogenic processes. The aim of this study was to investigate mRNA expression of 12- and 15-lipoxygenases (ALOX12, ALOX12B, ALOX15, ALOX15B) and the atypical ALOXE3 in human carotid atherosclerotic lesions, in relation to cerebrovascular symptoms and risk factors.

The Biobank of Karolinska Endarterectomies (BiKE) collection of human carotid plaque tissue and associated clinical data was utilized (n=132). Lesion mRNA levels were analyzed by TaqMan qPCR (n=132) and microarray hybridization (n=77).

Of the investigated mRNAs, only ALOX15B (also known as 15-LOX-2 or epidermis-type 15-LOX) was robustly detected in all plaque samples by qPCR, and thus suitable for quantitative statistical evaluation. Other ALOX mRNAs were detected with lower frequency and at lower levels, or virtually undetected. Microarray analysis confirmed ALOX15B as the most abundant 12- or 15-lipoxygenase mRNA in carotid lesions. Comparing plaques with or without attributable cerebrovascular symptoms (amaurosis fugax, TIA, or stroke), ALOX15B mRNA levels were higher in symptomatic than asymptomatic plaques (1.31 [1.11-1.56], n=102; and 0.79 [0.55-1.15], n=30, respectively; p=0.008; mean [95% CI], arbitrary units). Multivariate analysis confirmed symptomatic/asymptomatic status as a significant determinant of ALOX15B mRNA levels, independently of potentially confounding factors. Immunohistochemical and lipidomic analyses of a limited number of lesions confirmed the presence of ALOX15B protein and products in plaque tissue.

In summary, we observed associations between high ALOX15B expression in carotid lesions and cerebrovascular symptoms. Although not allowing conclusions on causal relationships, these findings suggest a link between ALOX15B and atherothrombotic events that merits further investigation.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov