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Title:   Author  item
                                            Bolster, Carlitem
                                            Cook, Kimberly

                                                         - *Kim*item
                                            MARCUS, IAN - University Of Californiaitem
                                            HAZNEDAROGLU, BERAT - University Of Californiaitem
                                            WALKER, SHARON - University Of California
  Submitted to: ASA-CSSA-SSSA Annual Meeting Abstracts
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 4/28/2010
                                    Publication  Date: 11/1/2010
                                    Citation: Bolster, C.H., Cook, K.L., Marcus, I.M., Haznedaroglu, B.Z., Walker, S.L. 2010. Correlating attachment behavior with cell properties for eight Porcine Escherichia coli Isolates. ASA-CSSA-SSSA Annual Meeting Abstracts.Interpretive Summary:
                                Technical Abstract: In this study we investigate how growth stage and depositional environment affect variability of cell properties and transport behavior of eight porcine E. coli isolates. We compared the surface properties and transport behavior for cells harvested at two different growth stages (exponential and stationary) and depositional environments (favorable and unfavorable). We then investigated correlations between measured cell properties and fitted bacterial attachment efficiencies. For both growth stages we found that bacterial attachment efficiencies to negatively-charged quartz sand varied among the eight different isolates by over an order of magnitude whereas attachment efficiencies to the positively-charged Fe-coated sands varied by a factor of less than two. With the exception of one isolate, growth condition had minimal impact on attachment efficiencies to the uncoated sands. A strong and statistically significant inverse relationship was observed between bacterial attachment to uncoated quartz sand and log-transformed zeta potential whereas a mild yet statistically significant relationship between bacterial attachment to the Fe-coated soils and cell width was observed. For the experimental conditions used in our study, we found that variability in E. coli transport was more dependent on the depositional environment than on growth conditions.


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