U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Virus and Prion Research: Ames, IA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area Ames, Iowa National Animal Disease Center Virus and Prion Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Nicholson, Tracyitem
                                            Brockmeier, Susanitem
                                            Loving, Crystal
  Submitted to: American Society for Microbiology Meeting
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 2/22/2008
                                    Publication  Date: 6/1/2008
                                    Citation: Nicholson, T.L., Brockmeier, S., Loving, C.L. 2008. Contribution of Bordetella bronchiseptica Filamentous Hemagglutinin and Pertactin to Respiratory Disease in Swine [abstract]. American Society for Microbiology Meeting, June 2-4, 2008, Boston, Massachusetts. 2008 CDROM.Interpretive Summary:
                                Technical Abstract: Respiratory disease in pigs is the most important health concern for swine producers today. Bordetella bronchiseptica is widely prevalent in swine populations and has multiple roles in respiratory disease. It is the primary etiologic agent of atrophic rhinitis, bronchopneumonia in young pigs, and has been demonstrated to increase the frequency and severity of respiratory disease due to other viral and bacterial pathogens. Most studies addressing virulence factors of B. bronchiseptica are based on isolates derived from hosts other than pigs. Swine isolates of B. bronchiseptica possess unique genetic and phenotypic traits as compared to isolates from other host species. Two well-studied virulence factors implicated in the adhesion process are filamentous hemagglutinin (FHA) and pertactin (PRN). We hypothesized that both FHA and PRN would serve critical roles in the adhesion process and be necessary for colonization of the swine respiratory tract. To investigate the role of FHA and PRN in Bordetella pathogenesis in swine, we constructed mutants containing an in-frame deletion of the FHA or the PRN structural gene in KM22, a virulent B. bronchiseptica swine isolate. We compared both of these mutants to KM22, the wild-type swine isolate, for their ability to colonize and cause disease. Except for day one post-infection, colonization of the PRN mutant was indistinguishable from wild-type and caused similar disease severity. In contrast, colonization of the FHA mutant was lower than wild-type at all respiratory tract sites and time points examined and caused limited to no disease. Our results indicate that FHA, but not PRN, is required for optimal colonization throughout the respiratory tract, including the nasal cavity, in swine.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov