U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Virus and Prion Research: Ames, IA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area Ames, Iowa National Animal Disease Center Virus and Prion Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Nicholson, Ericitem
                                            YOUNG, ALAN - SOUTH DAKOTA STATE UNIVERitem
                                            Richt, Juergen
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 9/29/2004
                                    Publication  Date: 10/14/2004
                                    Citation: Nicholson, E.M., Young, A.J., Richt, J. 2004. Establishment of a real time-PCR based assay for the determination of ovine PrP mRNA [abstract]. Animal Prion Diseases and the Americas. p. 90.Interpretive Summary:
                                Technical Abstract: Transmissible spongiform encephalopathies (TSEs) comprise a group of fatal neurodegenerative diseases that are characterized by accumulation of an abnormal form of the prion protein (PrP), termed PrPSc, an isoform of the normal host protein PrPC.  Scrapie in sheep has been recognized for over 200 years, and the disease is present world-wide with the exception of New Zealand and Australia. Despite the presence of known susceptible and resistant sheep PrP genotypes, the disease process remains poorly understood. Knowledge of the expression levels of PrP could contribute to a better understanding of the mechanisms involved in susceptibility and resistance to scrapie.  Here we describe a sensitive real time-PCR based assay utilizing SYBR Green technology for the determination of the ovine PrP mRNA.  This assay is currently being utilized to study tissue specific expression of PrP in sheep with resistant and susceptible genotypes.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov