U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 U.S. Dairy Forage Research Center: Madison, WI
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                                    Software
                People

                    Staff Listing
                                    Careers


        


                    Archives
                        Presentations
                                    World Dairy Expo
                                    Newsletters and Research Summaries
                    Resources
                        General Resources
                                    Books
                                    Teacher's Resources
                                    New Research Dairy Updates
                                    Integrated Research at USDFRCSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area Madison, Wisconsin U.S. Dairy Forage Research Center Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications
- IACUC Summaries - Milwaukee, WI Aquaculture
- IACUC Summaries - US Dairy Forage Research Center

Title:   Author  item
                                            Hall, Mary Bethitem
                                            LARSON, COLLEEN - UNIVERSITY OF FLORIDA
  Submitted to: Journal of Dairy Science
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 2/5/2005
                                    Publication  Date: 7/26/2005
                                    Citation: Hall, M. B., Larson, C. C. 2004. Effects of nonfiber carbohydrate source and protein degradability on ruminal protein fractions and NDF disappearance [abstract]. Journal of Dairy Science 87 Suppl. (1):463.Interpretive Summary:
                                Technical Abstract: Effects of nonfiber carbohydrate source (NFC) and protein degradability (RUP) on concentrations (mM) of ammonia (NH3), amino acid (AA), and branch chain VFA (BCVFA) in ruminal fluid, and on in situ disappearance of NDF from sorghum silage were evaluated using 6 ruminally cannulated Holstein cows in a three period (21 d) partially balanced incomplete latin square design with a 3x2 factorial arrangement of treatments. Dietary treatments included three NFC sources (ground corn=starch=ST; molasses+sucrose=sugar=SU; and citrus pulp=soluble fiber+sugar=SF) and two concentrations of ruminally degradable protein (+or-RUP) achieved by addition or omission of expeller soybean meal. Total mixed rations were formulated to be isonitrogenous and offered ad libitum. Sorghum silage (2 mm Wiley mill grind) was incubated in situ in polyester bags (53 ± 10 μm pore size) inserted via the rumen cannula on days 16, 17, and 18 at intervals to allow incubation times of 0, 6, 12, 18, 24, 30, and 48 h; residual NDF was measured. Ruminal fluid samples were collected on day 20 just prior to feeding and hourly for 12 h. Protocols were applied in each period. Significance was declared at P#88040.05; values presented are least squares means. NH3 differed only for RUP x hr in hours 1 through 3 post-feeding, with -RUP greater than +RUP. AA differed by NFC in hr 1 through 3, with ST less than SU + SF, and for interactions of NFC x hr and RUP x hr for all hours. BCVFA differed by NFC source with ST greater than SU+SF, and SF tended to be greater than SU (P=0.07). BCVFA tended to differ by RUP x hr (P=0.10). In situ disappearance of NDF differed for NFC and NFC x RUP in hours 6, 18, 24, and 30, and tended to differ for NFC x RUP at 48 h (P=0.054). Differences did not appear to be solely due to ruminal pH. Differences in ruminal AA and BCVFA suggest that ruminal protein digestion or use differs by NFC source. Differences in in situ disappearance of NDF suggest that in situ results are not likely to be uniform across diets and may be best suited for relative evaluation of NDF digestibility.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov