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Title:   Author  item
                                            Hughes, Stephenitem
                                            Doll, Kenneth

                                                         - *Ken*item
                                            Moser, Bryanitem
                                            BANG, SOOKIE - South Dakota School Of Mines And Technologyitem
                                            Rich, Josephitem
                                            Bischoff, Kennethitem
                                            Liu, Siqingitem
                                            Qureshi, Nasibitem
                                            Hector, Ronald

                                                         - *Ron*item
                                            Dien, Bruceitem
                                            Cotta, Michaelitem
                                            JONES, MARGE - Illinois State University
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 1/27/2009
                                    Publication  Date: 1/27/2009
                                    Citation: Hughes, S.R., Doll, K., Moser, B., Bang, S., Rich, J., Qureshi, N., Hector, R., Dien, B., Cotta, M., Bischoff, K., Liu, S., Jones, M. 2009. Automated production GMAX-L strains of Saccharomyces cereviciae for profitable sustainable cellulosic ethanol production combined with valuable coproduct isolation in mixed biorefinery settings [abstract]. LabAutomation 2009. Abstract No. 109. p. 42.Interpretive Summary:
                                Technical Abstract: Second generation cellulosic ethanol production is beginning in a variety of formats and using various ethanologenic microbes.  The best possibility would be to produce cellulosic ethanol using a Saccharomyces cereviciae that has been engineered to produce ethanol from pentose as well as hexose sugars.  A GMAX-L cellulosic Saccharomyces cerevisiae strain is under development to use xylose and glucose to produce ethanol from cellulosic hydrolysate and then biodiesel fethyl esters from corn oil and ethanol at the same biorefinery.  This strain produces 10-15% more ethanol than a glucose-utilization strain.  S. cerevisiae engineered to use xylose and glucose can produce more ethanol than the next best fungal or bacterial ethanologens.  Additional work will be performed on this new S. cerevisiae cellulosic ethanologen to express genes that will allow arabinose utilization.  Profitability is a major problem with the initial cellulosic refineries since they require expensive and complex assortments of enzymes, both cellulases and hemicellulases, to break down the lignocellulose.  For these second generation crossover biorefineries to be sustainable profitable operations they need to overcome the problem of high-cost enzymes to break down lignocellulosic feedstocks.  Expressing these enzymes in the cellulosic S. cerevivsiae could dramatically reduce the cost of enzymes required and be used for all ethanol production settings with the benefit of having a secondary valuable biofuel produced.


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