U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Infectious Bacterial Diseases Research: Ames, IA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area Ames, Iowa National Animal Disease Center Infectious Bacterial Diseases Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            LYASHCHENKO, K - CHEMBIO DIAGNOSTIC SYS.item
                                            GREENWALD, R - CHEMBIO DIAGNOSTIC SYS.item
                                            ESFANDIARI, J - CHEMBIO DIAGNOSTIC SYS.item
                                            CHAMBERS, M - VET. LAB. AGENCY, UKitem
                                            VICENTE, J - INSTI. DE INVEST. SPAINitem
                                            GORTAZAR, C - INSTI. DE INVEST. SPAINitem
                                            SANTOS, N - UNIV. OF MINHO, PORTUGALitem
                                            CORREIA-NEVES, M - UNIV. OF MINHO, PORTUGALitem
                                            BUDDLE, B - HOPKIRK RES. INST., NZitem
                                            JACKSON, R - MASSEY UNIV. NEW ZEALANDitem
                                            O'BRIEN, D - DEPT. OF NAT. RES., MIitem
                                            SCHMITT, S - DEPT. OF NAT. RES., MIitem
                                            Palmer, Mitchellitem
                                            DELAHAY, R - CENTRAL SC. LAB., UKitem
                                            Waters, Wade
  Submitted to: Veterinary Microbiology
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 5/26/2008
                                    Publication  Date: 12/10/2008
                                    Citation: Lyashchenko, K.P., Greenwald, R., Esfandiari, J., Chambers, M.A., Vicente, J., Gortazar, C., Santos, N., Correia-Neves, M., Buddle, B.M., Jackson, R., O'Brien, D.J., Schmitt, S., Palmer, M.V., Delahay, R.J., Waters, W.R. 2008. Animal-side Serologic Assay for Rapid Detection of Mycobacterium bovis Infection in Multiple Species of Free-ranging Wildlife. Veterinary Microbiology. 132(3-4):283-292.Interpretive Summary: White-tailed deer, brush-tailed possums, Eurasian badger, and feral swine are wildlife reservoirs of bovine tuberculosis. The presence of disease in these reservoir hosts seriously threatens ongoing efforts to eradicate tuberculosis from cattle. A reliable and easy blood test applicable to multiple species would be beneficial to tuberculosis eradication and control programs around the world. In the present study, it was determined that deer, possum, badger and feral swine naturally and/or experimentally infected with tuberculosis produce antibodies to the tuberculosis agent and that these antibodies are detectable by simple laboratory methods, including a test kit suitable for field use. These findings will be useful for the control of bovine tuberculosis in reservoir hosts, thus, benefiting the cattle industry and potentially decreasing the spread of tuberculosis from the reservoir to cattle.
                                Technical Abstract: Numerous species of wild mammals are susceptible to Mycobacterium bovis, a cause of bovine tuberculosis (TB). Eurasian badgers, white-tailed deer, brushtail possums, and wild boar are implicated in the maintenance of wildlife reservoirs of M. bovis infection in different countries, fueling bovine TB in domestic livestock. Rapid and accurate assays that can detect TB in a variety of species under field conditions are needed to improve wildlife surveillance efforts worldwide. Using two membrane-based serological assays, a lateral-flow rapid test (RT) and MultiAntigen Print ImmunoAssay (MAPIA), antibody responses during M. bovis infection in the four natural hosts of were characterized. Predominantly recognized antigens were identified and heterogeneity of antibody responses in each species was shown. The utility of RT as a single serologic assay for detection of bovine TB in badgers, deer, possums, and wild boar was demonstrated. The diagnostic accuracy of RT varied from 72% in possums to 97% in deer. Use of whole blood from white-tailed deer demonstrated the potential of this approach with RT for an expedited detection of M. bovis infection in wildlife populations under field conditions. A close association between antibody responses and the presence of gross TB lesions found in wild boar suggested that RT could predominantly identify animals with most advanced disease, which have the greatest potential of shedding M. bovis. Therefore, serologic assays may provide useful screening tools for controlling bovine TB in multi-species wildlife populations.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov