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Title:   Author  item
                                            CHIANG, HSIN-I - TEXAS A&M UNIVERSITYitem
                                            Swaggerty, Christina

                                                         - *Christi*item
                                            Kogut, Michael

                                                         - *Mike*item
                                            ZHOU, HUAIJUN - TEXAS A&M UNIVERSITY
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 10/1/2008
                                    Publication  Date: 1/10/2009
                                    Citation: Chiang, H., Swaggerty, C.L., Kogut, M.H., Zhou, H. 2009. Gene expression profiling in Salmonella enterica serovar Typhimurium stimulated heterophils using a chicken 44k agilent microarray [abstract]. Plant & Animal Genome XVII Conference, January 10-14, 2009, San Diego, California. p. 513.Interpretive Summary:
                                Technical Abstract: Microarray technology is a powerful high-throughput tool for the analysis of host-pathogen interactions that permits ascribing the transcriptional status of thousands of genes simultaneously.  To determine the host response to Salmonella Typhimurium (ST) in heterophils, a whole chicken genome array was used to analyze RNA isolated from heterophils and then stimulated in vitro with ST (30 min, 1 h, 2 h, and 3 h) or medium control (NS).  A dual-color balanced design was used to provide a direct comparison between ST-stimulated and non-stimulated controls (30 min vs. NS (30M/NS), 1 h vs. NS (1HR/NS), 2 h vs. NS (2HR/NS) and 3 h vs. NS (3HR/NS)).  Each comparison includes four biological replicates.  There were 2791, 3744, 12947, and 12482 genes differentially expressed in the comparisons of 30M/NS, 1HR/NS, 2HR/NS and 3HR/NS, respectively (P less than 0.001), while 50, 82, 153, and 146 genes were related to immune function, respectively.  More than 80% of immune-related genes overlapped between the comparisons (30M/NS vs. 1HR/NS, 1HR/NS vs. 2HR/NS, and 2HR/NS vs. 3HR/NS).  These candidate genes include cytokines [e.g. Interleukin (IL) 1beta, -6, -10B and -12B], chemokines (e.g. IL-8, CCL4, K60, and K203), and cluster of differentiation (CD) markers (e.g. CD44, -47 and -83).  A relatively large increase in the number of differentially expressed immune-related genes was observed between 1 h and 2 h post-stimulation.  The results of continuous transcriptional change discovered in the present study provide important information to elucidate both the cellular and molecular mechanisms of ST infection in chickens.


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