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Title:   Author  item
                                            SCHILLER, IRENE - Prionics Agitem
                                            SCHRODER, B - Prionics Agitem
                                            VORDERMEIER, H - Veterinary Laboratories Agency (VLA)item
                                            Waters, Wadeitem
                                            Palmer, Mitchellitem
                                            Thacker, Tyleritem
                                            WHELAN, ADAM - Veterinary Laboratories Agency (VLA)item
                                            HARDEGGER, ROLAND - Prionics Agitem
                                            MARG-HAUFE, BEATRICE - Prionics Agitem
                                            RAEBER, ALEX - Prionics Agitem
                                            OESCH, BRUNO - Prionics Ag
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 8/25/2009
                                    Publication  Date: 8/25/2009
                                    Citation: Schiller, I., Schroder, B., Vordermeier, H.M., Waters, W.R., Palmer, M.V., Thacker, T.C., Whelan, A., Hardegger, R., Marg-Haufe, B., Raeber, A., Oesch, B. 2009. Assessment of OmpATb as a Novel Antigen for the Diagnosis of Bovine Tuberculosis [abstract].Interpretive Summary:
                                Technical Abstract: In search for better tools to control bovine tuberculosis, the development of diagnostic tests with improved specificity and sensitivity has a high priority. We chose to search for novel immunodiagnostic reagents. In this study, Rv0899 (Outer membrane protein A of Mycobacterium tuberculosis, OmpATb) was evaluated as a stimulation antigen in a IFN-gamma release assay to diagnose bovine tuberculosis. OmpATb induced IFN-gamma responses in cattle experimentally infected with M. bovis as early and as persistently as ESAT-6 and CFP-10, the current lead diagnostic antigens. In naturally infected cattle, OmpATb stimulated IFN-gamma production in 22 of 26 animals (85%). Importantly, OmpATb detected a portion of M. bovis-infected cattle which did not respond to ESAT-6 and CFP-10 (5 of 6 cattle). The combined diagnostic sensitivity of OmpATb, ESAT-6 and CFP-10 in a pre-selected group consisting of naturally TB-infected cattle with an overrepresentation of ESAT-6/CFP-10 non-responsers was 96% (25 of 26 animals). Specificity of OmpATb in uninfected cattle was 100% (27 cattle tested, 12 of them reacted false positive with tuberculins). In summary, our results indicate that OmpATb has the potential to enhance the sensitivity of previously described diagnostic tests based on ESAT-6 and CFP-10, and that the combined use of OmpATb, ESAT-6, CFP-10 and other proteins may achieve at least equal sensitivity than PPDs yet, at higher specificity. Further studies evaluating the diagnostic performance of OmpATb in combination with other proteins are ongoing.


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