U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Food and Feed Safety Research: College Station, TX
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Plains Area College Station, Texas Southern Plains Agricultural Research Center Food and Feed Safety Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            LI, XIANYAO - TEXAS A&M UNIVERSITYitem
                                            Swaggerty, Christina

                                                         - *Christi*item
                                            Kogut, Michael

                                                         - *Mike*item
                                            CHIANG, HSIN-I - TEXAS A&M UNIVERSITYitem
                                            YING, WANG - TEXAS A&M UNIVERSITYitem
                                            Genovese, Kenneth

                                                         - *Ken*item
                                            He, Louisitem
                                            PEVZNER, IGAL - COBB-VANTRESS, INC.item
                                            ZHOU, HUAIJUN - TEXAS A&M UNIVERSITY
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 10/1/2008
                                    Publication  Date: 1/10/2009
                                    Citation: Li, X., Swaggerty, C.L., Kogut, M.H., Chiang, H., Ying, W., Genovese, K.J., He, H., Pevzner, I., Zhou, H. 2009. Genome-wide gene expression profiling of chicken spleen responding to Campylobacter jejuni infection in broilers [abstract]. Plant & Animal Genome XVII Conference, January 10-14, 2009, San Diego, California. p. 511.Interpretive Summary:
                                Technical Abstract: Campylobacter jejuni (C. jejuni) is a zoonotic pathogen that causes human diarrhea worldwide.  Chickens are a natural reservoir of C. jejuni.  Understanding the host response to C. jejuni infection at the molecular level will lay the foundation to control human campylobacterosis by reducing food contamination.  Two distinct genetic lines, resistant (line A) and susceptible (line B) to C. jejuni colonization, were utilized to profile the host response to C. jejuni infection using an Agilent chicken 44K microarray.  Day-old chickens were challenged orally with C. jejuni and spleens collected for total RNA 7 days post-challenge.  Twenty infected samples with highest (a) or lowest bacterial number (b) in cecal content and twenty non-infected (c) in each line were randomly pooled into four biological replicates.  The pair comparisons among these three groups within each line were analyzed.  The signal intensity of each gene was normalized using LOWESS method and a mixed model was used to identify differentially expressed genes by SAS (P less than 0.001).  This was opposite to previous cecal tonsil microarray result.  There were 468, 743, and 939 genes differentially expressed between groups a and c, groups a and b, and groups b and c in line A, respectively, and 201, 37, 126 genes in line B, respectively.  More differentially expressed genes in spleen in line A than in line B were found.  The results indicated that significantly different response to C. jejuni infection occurred between resistant and susceptible chicken lines, and the effects of interaction between genetics and tissue should be considered.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov