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ARS Home Plains Area Clay Center, Nebraska U.S. Meat Animal Research Center Meat Safety and Quality Research Publications at this Location





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Title:   Author  item
                                            Wells, James

                                                         - *Jim*item
                                            Shackelford, Stevenitem
                                            Berry, Elaineitem
                                            Kalchayanand, Norasak

                                                         - *Nor*item
                                            Varel, Vincentitem
                                            Arthur, Terranceitem
                                            Bosilevac, Joseph

                                                         - *Mick*item
                                            Freetly, Harveyitem
                                            Wheeler, Tommyitem
                                            Ferrell, Calvinitem
                                            KOOHMARAIE, MOHAMMAD - FORMER ARS EMPLOYEE
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 2/18/2009
                                    Publication  Date: 4/22/2009
                                    Citation: Wells, J., Shackelford, S.D., Berry, E.D., Kalchayanand, N., Varel, V.H., Arthur, T.M., Bosilevac, J.M., Freetly, H.C., Wheeler, T.L., Ferrell, C.L., Koohmaraie, M. 2009.  Prevalence and Level of Escherichia coli O157:H7 in Feces and On Hides of Feedlot Steers Fed Diets With or Without Wet Distillers Grains With Solubles [abstract]. Microbial Ecology 57(3):583-584.Interpretive Summary: No interpretive summary is required.
                                Technical Abstract: Studies have indicated distillers grains in cattle diets may alter the shedding of Escherichia coli O157:H7 in feces, thereby increasing the E. coli O157:H7 load on the hide and ultimately on the carcass.  To determine if wet distillers grains with solubles (WDGS) in the diet affected E. coli O157:H7 in growing and finishing cattle, weaned steers (n = 603) were blocked by genetic lines and weaning weight, and randomly assigned to dietary treatments (with and without WDGS).  At approximately 53 days after weaning, steers were sorted into 8 pens (4 pens per treatment) with 75 to 77 steers per pen.  Growing diets with 0% or 13.9% WDGS (as %DM) were fed beginning on d 0 and finishing diets with 0% or 40% WDGS were fed beginning on d 78.  Hide and fecal samples were collected from each animal for enumeration and enrichment of E. coli O157:H7 from October through June.  In the growing phase, the fecal prevalence for E. coli O157:H7 was twice that in animals fed diets with 13.9% WDGS (P < 0.001), but neither the percentage nor the distribution of E. coli O157:H7 enumerable samples in feces was different for the two diets.  In the finishing phase, average fecal prevalence for cattle fed 40% WDGS was 14.9% compared to 1.5% for animals fed corn (P < 0.001), and hide prevalence was 32.8 and 9.2% (P < 0.001), respectively.  The average percentage of fecal E. coli O157:H7 enumerable samples during the finishing phase for cattle fed 40% WDGS was 2.7% compared to 0.1% for corn (P < 0.001).  The average percentage of E. coli O157:H7 enumerable hide samples was not different (1.9 vs 0.0%, P < 0.06), but the distributions of these counts were different (P < 0.05).  Animals fed 40% WDGS had higher levels of generic E. coli (P < 0.001), higher pH (P < 0.001), and lower concentrations of L-lactate (P < 0.001) in their feces.  Within cattle fed 40% WDGS, fecal shedding of E. coli O157:H7 was not associated with fecal pH.  However, fecal shedding of E. coli O157:H7 from the cattle fed 40% WDGS was associated with lower levels of fecal generic E. coli (6.94 vs 7.06 log10 CFU per g, P < 0.05) and higher fecal concentrations of L-lactate (1.87 vs 1.39 mM, P < 0.01).  These results indicate that feeding 40% WDGS (DM basis) may increase the level and prevalence of E. coli O157:H7 in feedlot cattle, although the magnitude of the difference in this study may have been affected by low prevalence in the control pens.


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