U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Crop Production and Pest Control Research: West Lafayette, IN
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area West Lafayette, Indiana Crop Production and Pest Control Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications
- Small Grains Viral Disease Laboratory
- Corn and Sorghum Fungal Disease Laboratory
- Host Plant Resistance to Insects Laboratory
- Wheat/Hessian fly Interactions Laboratory
- Small Grains Fungal Disease Laboratory
- Soybean Quality Improvement Laboratory
- Uniform Soybean Tests, Northern Region

Title:   Author  item
                                            KEMA, GHJ - Plant Research International - Netherlandsitem
                                            Goodwin, Stephen

                                                         - *Steve*item
                                            VAN DER LEE, TAJ - Plant Research International - Netherlandsitem
                                            DHILLON, B - Purdue Universityitem
                                            ARANGO, R - Plant Research International - Netherlandsitem
                                            Crane, Charlesitem
                                            DIAZ, C - Coconut Research Institute Of Sri Lankaitem
                                            SOUZA, M - Embrapa-Labexitem
                                            CARLIER, J - Centro De Cooperation Internationale En Recherche Agronomique Pour Le Development (CIRAD)item
                                            SCHMUTZ, J - Hudson Control Groupitem
                                            GRIGORIEW, IV - Department Of Energy Joint Genome
  Submitted to: Meeting Proceedings
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 2/5/2010
                                    Publication  Date: 3/30/2010
                                    Citation: Kema, G., Goodwin, S.B., Van Der Lee, T., Dhillon, B., Arango, R., Crane, C.F., Diaz, C., Souza, M., Carlier, J., Schmutz, J., Grigoriew, I. 2010. The Draft Genome Sequence of Mycosphaerella fijiensis, the Black Sigatoka Pathogen of Banana. Meeting Proceedings.Interpretive Summary:
                                Technical Abstract: Mycosphaerella fijiensis is a fungal pathogen of banana and the causal agent of the devastating Black Sigatoka or black leaf streak disease. Its control requires weekly fungicide applications when bananas are grown under disease-conducive conditions, which mostly represent precarious tropical environments. We started a multidisciplinary research program on M. fijiensis that is aiming at pesticide reduction. The first goal was to collect genomic data and to develop tools for molecular analysis of this pathosystem. Analyses of electrophoretic karyotypes on DNA extracted from protoplasts of M. fijiensis showed that chromosome sizes range between 500 kb and ~12 Mb. A genetic linkage map comprising 19 linkage groups covering 1417 cM was calculated using high LOD scores. All markers were sequenced and aligned to the draft 7.8x whole genome shotgun Sanger sequence of M. fijiensis CIRAD86. In addition more than 30,000 ESTs from three in vitro libraries were sequenced. The genome has an estimated size of 74 Mb and in 56 scaffolds covering more than 99% of the genome. The largest scaffold is 11.8 Mb in length and 28 scaffolds (99.8. %) are larger than 50 Kb. The genome size of M. fijiensis is 80% larger than that of M. graminicola mostly due to additional repeated sequences. The current draft release, version 1.0, includes a total of 10,327 gene models predicted and functionally annotated using the JGI annotation pipeline. The availability of the M. fijiensis genome will greatly assist future studies aimed at the control of black leaf streak disease as well as genomic comparisons with many other agronomically important Dothideomycetes fungi that currently are being sequenced through the Fungal Genome Program at the U.S. Department of Energy’s Joint Genome Institute.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov