U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Crop Production and Pest Control Research: West Lafayette, IN
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area West Lafayette, Indiana Crop Production and Pest Control Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications
- Small Grains Viral Disease Laboratory
- Corn and Sorghum Fungal Disease Laboratory
- Host Plant Resistance to Insects Laboratory
- Wheat/Hessian fly Interactions Laboratory
- Small Grains Fungal Disease Laboratory
- Soybean Quality Improvement Laboratory
- Uniform Soybean Tests, Northern Region

Title:   Author  item
                                            Scofield, Steven

                                                         - *Steve*item
                                            Brandt, Amanda
  Submitted to: Methods in Molecular Biology
                                    Publication Type: Book / Chapter
                                    Publication Acceptance Date: 4/10/2010
                                    Publication  Date: 6/11/2012
                                    Citation: Scofield, S.R., Brandt, A.S. 2012. Methods for Virus-Induced Gene Silencing in Hexaploid Wheat using barley stripe mosaic virus vectors. Methods in Molecular Biology. 894:93-112.Interpretive Summary: Virus-induced gene silencing (VIGS) is a useful functional genomics tool for rapidly creating gene knockout phenotypes that can be used to infer gene function.  Until recently, VIGS has only been possible in dicotyledonous plants.  However, the development of vectors based on barley stripe mosaic virus (BSMV) has now made VIGS possible in barley and wheat.  VIGS has particular advantages for functional genomics in wheat, where the organism’s hexaploidy and recalcitrance to transformation have greatly hindered strategies for functional identification of genes.  Here methods are presented for using the Barley stripe mosaic virus VIGS system (BSMV-VIGS) to silence genes in hexaploid wheat.
                                Technical Abstract: Virus-induced gene silencing (VIGS) is a useful functional genomics tool for rapidly creating gene knockout phenotypes that can be used to infer gene function.  Until recently, VIGS has only been possible in dicotyledonous plants.  However, the development of vectors based on barley stripe mosaic virus (BSMV) has now made VIGS possible in barley and wheat.  VIGS has particular advantages for functional genomics in wheat, where the organism’s hexaploidy and recalcitrance to transformation have greatly hindered strategies for functional identification of genes.  Here methods are presented for using the Barley stripe mosaic virus VIGS system (BSMV-VIGS) to silence genes in hexaploid wheat.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov