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Title:   Author  item
                                            Swaggerty, Christina

                                                         - *Christi*item
                                            CHIANG, H - TX A&M UNIVERSITYitem
                                            ZHOU, H - TX A&M UNIVERSITYitem
                                            LI, X - TX A&M UNIVERSITYitem
                                            PEVZNER, IGAL - COBB-VANTRESS, INCitem
                                            Kogut, Michael

                                                         - *Mike*
  Submitted to: Society for Leukocyte Biology  Meetings   Proceedings
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 7/2/2007
                                    Publication  Date: 10/11/2007
                                    Citation: Swaggerty, C.L., Chiang, H.I., Zhou, H., Li, X., Pevzner, I.Y., Kogut, M.H. 2007. Gene expression profiling of heterophils from Salmonella-resistant and-susceptible chickens using a 44K agilent microarray [abstract]. In: Proceedings of the Society for Leukocyte Biology, October 11-13, 2007, Cambridge, Massachusetts. p. 20.Interpretive Summary:
                                Technical Abstract: We previously examined in vitro heterophil function and resistance/susceptibility to in vivo challenge with Salmonella enteritidis (SE) in broilers.  All evaluations have shown a dichotomy of innate immune responsiveness with line A more responsive with increased ability to phagocytize, degranulate, produce an oxidative burst response, and mRNA expression of pro-inflammatory cytokines compared to line B.  The in vitro studies translated to increased resistance (A) and susceptibility (B) in in vivo challenges with SE.  Microarrays allow us to perform large-scale expression profiling to ascribe biological function and interactions between genes with available genomic sequences.  Heterophils were isolated from line A and B chickens, stimulated with SE, and RNA isolated and analyzed with a chicken 44K Agilent microarray.  A dual-color balanced design provided a direct comparison between SE-treated and control heterophils (A-SE vs. A-Con; B-SE vs. B-Con) and between lines (A-Con vs. B-Con; A-SE vs. B-SE).  There were more (P<0.05) genes expressed in A-Con (4377) compared to B-Con (4333) heterophils. Of those, 71 and 69, respectively, were immune-related.  Following exposure to SE, fewer (P<0.05) genes were expressed in A (3096) compared to B (3312).  Even though fewer total genes were expressed in SE-treated line A heterophils, 67 were immune-related compared to 56 for line B.  These results provide data to dissect the genes associated with resistance against SE in chickens.


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