U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Natural Products Utilization Research: Oxford, MS
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Location Research Units
                        National Sedimentation Laboratory
                                    Natural Products Utilization Research Unit
                                    Water Quality and Ecology Research Unit
                                    Watershed Physical Processes Research UnitSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Southeast Area Oxford, Mississippi Natural Products Utilization Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Cantrell, Charlesitem
                                            Schrader, Kevinitem
                                            MAMONOV, L - ALMATY, KAZAKHSTANitem
                                            SITPAEVA, G - ALMATY, KAZAKHSTANitem
                                            KUSTOVA, T - ALMATY, KAZAKHSTANitem
                                            DUNBAR, C - UNIVERSITY OF MISSISSIPPIitem
                                            Wedge, David
  Submitted to: Journal of Agricultural and Food Chemistry
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 8/12/2005
                                    Publication  Date: 9/13/2005
                                    Citation: Cantrell, C.L., Schrader, K., Mamonov, L.K., Sitpaeva, G.T., Kustova, T.S., Dunbar, C., Wedge, D.E. 2005. Isolation and identification of antifungal and antialgal alkaloids from haplophyllum sieversii. Journal of Agricultural and Food Chemistry.  53(20):7741-7748.Interpretive Summary: The desire for safe and more effective agrochemicals with reduced environmental and/or mammalian toxicity remains important.  Essential to these efforts is the identification of new promising compounds possessing high levels of desirable biological activities and reduced harm to the environment.  As part of our current research program, screening of plants native to the Republic of Kazakhstan for the presence of antifungal and antialgal constituents lead us to an investigation of Haplophyllum sieversii.  Antifungal and antialgal natural compounds have been isolated from H. sieversii and tested for their ability to inhibit these organisms.
                                Technical Abstract: Bioassay-guided fractionation of the hexane:ethyl acetate:water (H:EtOAc:H2O) crude extract of the aerial parts of Haplophyllum sieversii was performed due to preliminary screening data that indicated the presence of growth inhibitory components against Colletotrichum fragariae, Colletotrichum gloeosporioides, Colletotrichum acutatum.  Fractionation was directed using bioautographical methods resulting in the isolation of the bioactive alkaloids flindersine, anhydroevoxine, haplamine, and a lignan eudesmin.  These four compounds were evaluated for activity against C. fragariae, C. gloeosporioides, C. acutatum, Botrytis cinerea, Fusarium oxysporum, and Phomopsis obscurans in a dose-response growth-inhibitory bioassay at 50.0, 100.0, and 150.0 uM.  Of the four compounds tested, flindersine demonstrated the highest level of antifungal activity.  Additionally, flindersine, eudesmin, and haplamine were screened against the freshwater phytoplanktons Oscillatoria perornata, Oscillatoria agardhii, Selenastrum capricornutum, and Pseudanabaena sp. (strain LW397).  Haplamine demonstrated selective inhibition against the odor-producing cyanobacterium O. perornata compared to activity against the green alga S. capricornutum, with lowest-observed-effect concentration values of 1.0 uM and 10.0 uM, respectively.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov