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Title:   Author  item
                                            SCHILLER, IRENE - PRIONICS AG, SWITZERLANDitem
                                            VORDERMEIER, MARTIN - VET. LAB. AGENCY, GBitem
                                            Waters, Wadeitem
                                            MARG-HAUFE, BEATRICE - PRIONICS AG, SWITZERLANDitem
                                            Palmer, Mitchellitem
                                            Thacker, Tyleritem
                                            HARDEGGER, ROLAND - PRIONICS AG, SWITZERLANDitem
                                            KYBURZ, ANNIKA - PRIONICS AG, SWITZERLANDitem
                                            RAEBER, ALEX - PRIONICS AG, SWITZERLANDitem
                                            OESCH, BRUNO - PRIONICS AG, SWITZERLAND
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 7/27/2007
                                    Publication  Date: 7/27/2007
                                    Citation: Schiller, I., Vordermeier, M., Waters, W.R., Marg-Haufe, B., Palmer, M.V., Thacker, T.C., Hardegger, R., Kyburz, A., Raeber, A., Oesch, B. 2007. Bovine Tuberculosis:  Analyzing the Parameters of the Interferon Gamma Assay and Improved Diagnosis with New Antigens [abstract].Interpretive Summary:
                                Technical Abstract: 
Bovine tuberculosis (TB), a zoonotic disease with a major economic impact, continues to be a significant problem with a global perspective. The BOVIGAM® interferon  gamma (IFN-gamma) assay constitutes a laboratory-based tuberculosis test and is widely used complementary to the tuberculin skin test. The assay consists of a first step culturing whole blood with antigens and stimulating leucocytes to produce IFN-gamma which is quantified by ELISA in a second step. The first step measures the cell-mediated immune response (CMI) and critically depends on the sample quality, stimulation reagents and culture conditions.
The CMI is known to be impacted negatively by stress. We have stimulated fresh blood from animals with or without stress with mitogens resulting in significantly lower IFN-gamma production in stressed animals and therefore potentially leading to false negative results. These results furthermore emphasize the utility of a positive control for stimulation. 
Tuberculosis-specific stimulation is currently done with tuberculins. We have analyzed tuberculins at different concentrations from different sources and will report on the optimal standardized tuberculin activity. As an alternative, a cocktail of recombinant antigens for stimulation resulted in improved diagnostic sensitivity and specificity. These recent developments in measuring the CMI therefore represent excellent tools for control and eradication of bovine tuberculosis.


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