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Title:   Author  item
                                            Johnson, Ericitem
                                            Dowd, Patrick
  Submitted to: American Society of Plant Biologists Annual Meeting
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 7/11/2007
                                    Publication  Date: 7/7/2007
                                    Citation: Johnson, E.T., Dowd, P.F. 2007. Excision of the piggyBac transposable element in maize cells is a precise event [abstract]. American Society of Plant Biologists Annual Meeting. Paper No. P36032Interpretive Summary:
                                Technical Abstract: The piggyBac transposable element (TE) from the moth Trichoplusia ni encodes a ‘cut and paste’ DNA transposase that has been used to transform a number of insects, as well as planaria, mammalian cells, and mice.  The wild type and a mutated piggyBac TE excised from a DNA vector in transient assays using maize cells when the piggyBac transposase was simultaneously expressed by a maize ubiquitin promoter.  The mutated piggyBac TE also excised in the presence of the piggyBac transposase in stably transformed maize cells, leaving behind a ‘TTAA’ DNA sequence in its place, indicating a precise molecular excision characteristic of piggyBac transposase in other eukaryotic cells.  These data indicate that piggyBac transposase may be able to carry genes into maize cells or serve as a mutagen for genetic studies.


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