U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 close



Research
                Research Home
                            National Programs
                            Research Projects
                            Scientific Manuscripts
                            International Engagement
                            Scientific Software/Models
                            Databases and Datasets
                            Office of Scientific Quality Review
            Media
                News & Features
                            Multimedia
                            Briefing Room
                            Down on the Farm
                            Press Room
                            Science in Your Shopping Cart
                            Scientific Discoveries
                            Tellus
                            Under the Microscope
            About ARS
                About ARS Home
                            Staff Directory
                            Labs and Research Centers (Map)
                            Headquarter Offices
                            Organizational Chart
                            Employee Services (REE Employees Only)
                            Office of Outreach, Diversity, and Equal Opportunity
            Work With Us
                Work With Us Home
                            ARS Vacancies at USAJOBS
                            Careers at ARS Information
                            Scientific Collaborations
                            Extramural Agreements
                            1890 Faculty Research Sabbatical ProgramSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            PARK, KYUNGJE - CHANGWON, KOREAitem
                                            PARK, DONGWOON - USDA,ARS, APDLitem
                                            KIM, CHUNHEE - CHANGWON, KOREAitem
                                            HAN, BEOM - AVICORE BIO INST. KOREA Ritem
                                            PARK, TAE - SEOUL NAT U, KOREAitem
                                            HAN, JAE - SEOUL NAT U, KOREAitem
                                            Lillehoj, Hyunitem
                                            KIM, JIN - CHANGWON, KOREA
  Submitted to: Biotechnology Letters
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 2/9/2005
                                    Publication  Date: 3/1/2005
                                    Citation: Park, K., Park, D., Kim, C., Han, B.K., Park, T.S., Han, J.Y., Lillehoj, H.S., Kim, J.K. 2005. Development and characterization of a recombinant chicken single-chain fv antibody detecting eimeria acervulina sporozoite antigen.Biotechnology Letters. 27:289-295.Interpretive Summary: Avian coccidiosis, caused by intestinal parasites belonging to the genus Eimeria, results in a significant economic loss to the poultry industry worldwide.  Although anti-coccidial drugs have been effective for the control of this disease, high costs and the increasing emergence of drug resistant parasites limit their use in the field. As an alternative strategy, vaccination using whole parasites has shown to induce strong protective host immunity against coccidiosis.  However, due to the complexity of the parasite life cycle and the existence of multiple species of Eimeria, host protective immunity induced by live parasite vaccines is limited and do not protect against field variants of Eimeria.  In this paper, ARS scientists in collaboration with scientists at Seoul National University and Changwon University developed a new immunological strategy which involves using   antibodies that actively block the invasion of parasites into host cells.  Using recombinant chicken antibodies designed to block parasite invasion of cells, these scientists propose a new intervention strategy to reduce coccidian contamination in the field. This is the first demonstration that show feasibility of using recombinant chicken antibodies to reduce coccidia infection.   This information will help poultry industry to develop a new method to control avian coccidiosis.
                                Technical Abstract: Chicken monoclonal antibody (mAb), 8C3, which is reactive with a sporozoite antigen of Eimeria acervulina, is a potential therapeutic agent against avian coccidiosis caused by Eimeria spp. However, production of it in cell culture requires improvement. Accordingly, recombinant single chain variable fragment (ScFv) antibody was constructed by amplification of the VH and VL genes from chicken hybridoma, 8C3 and when expressed in E. coli gave 5 mg l-1 antibody . The expressed protein had antigen binding activity equivalent to that of the parental mAb.  In addition, nucleotide sequence comparison to the germline chicken VL suggested that the gene conversion with V lambda pseudogenes might contribute to the diversification of VL genes in chickens.  Recombinant 8C3 antibody represents a valuable resource for avian immunology and parasitology.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov