U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Virus and Prion Research: Ames, IA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area Ames, Iowa National Animal Disease Center Virus and Prion Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Nicholson, Ericitem
                                            Greenlee, Justinitem
                                            Kunkle, Robertitem
                                            Hamir, Amirali
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 6/20/2009
                                    Publication  Date: 9/23/2009
                                    Citation: Nicholson, E.M., Greenlee, J.J., Kunkle, R.A., Hamir, A.N. 2009. Guanidinium Chloride Unfolding of PrP**Sc Exhibits Marked Differences Between Genotypes [abstract]. Prion 2009. p. 182.Interpretive Summary:
                                Technical Abstract: Background:  Various methods have been promoted as to their potential to differentiate TSE isolates (strains).  Traditionally a panel of inbred mice has been used to differentiate TSE isolates, and while this approach has proven rigorous it is slow.  Biochemical methods employed to date are rapid in comparison but for the most part are limited to proteinase K cleavage point differentiation as determined by the molecular weight of the protein on a Western blot.  This approach is used extensively with BSE and has been employed by various groups to reliably differentiate H-type, L-type, and classical BSE.  In the context of the protein only hypothesis, the differences must arise due to different folding parameters (structure, stability, or both).  It is not surprising then that denaturant unfolding of PrP**Sc monitored by circular dichroism has been used to differentiate various TSE isolates.  
    Methods:  Here we adapt denaturant unfolding of PrP**Sc to be monitored by a commercial, ELISA based diagnostic platform. 
    Results and Discussion:  Marked differences in denaturant unfolding parameters are observed between sheep of different genotypes inoculated with a single sheep scrapie isolate.  This result highlights the difficulty of adapting approaches developed for rodent models of TSEs to a natural host system as well as the potential for passage in a new host of different genotype to drive strain differentiation.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov