U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Infectious Bacterial Diseases Research: Ames, IA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area Ames, Iowa National Animal Disease Center Infectious Bacterial Diseases Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            GREEN, LAWRENCE - PRITEST INC.item
                                            JONES, CYNTHIA - PRITEST INC.item
                                            SHERWOOD, ANNE - PRITEST INC.item
                                            GARKAVI, INNA - PRITEST INC.item
                                            CANGELOSI, GERARD - PRITEST INC.item
                                            Thacker, Tyleritem
                                            Palmer, Mitchellitem
                                            Waters, Wadeitem
                                            RATHE, CHRIS - PRITEST INC.
  Submitted to: Clinical and Vaccine Immunology
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 7/6/2009
                                    Publication  Date: 9/1/2009
                                    Citation: Green, L.R., Jones, C.C., Sherwood, A., Garkavi, I., Cangelosi, G.A., Thacker, T.C., Palmer, M.V., Waters, W.R., Rathe, C. 2009. Single-Antigen Serological Testing for Bovine Tuberculosis. Clinical and Vaccine Immunology. 16(9):1309-1313.Interpretive Summary: Despite highly successful eradication efforts in several countries, tuberculosis of cattle remains a serious health concern worldwide. Recent outbreaks of tuberculosis in Michigan, Minnesota, California, Texas, and New Mexico demonstrate that the disease is far from eliminated from the United States. Improved techniques are needed for detection of infected cattle. In this study, a new test was developed and tested for the specific diagnosis of tuberculosis infection of cattle. Responses by cattle infected with the tubercle bacillus were distinguishable from responses by cattle infected with closely related bacteria. Knowledge obtained from this study will enable more accurate detection of cattle with tuberculosis, thereby, enhancing the tuberculosis eradication program.
                                Technical Abstract: Antibody responses are useful indicators of Mycobacterium bovis infection of cattle. Tests for serological responses often use panels of multiple M. bovis antigens as detection probes. This is recommended because responses to single antigens may be too variable for consistent diagnosis. However, the use of multiple antigens increases costs and the risk of cross reactivity with antibodies directed against orthologous antigens expressed by other species. An alternative approach is to use a single antigen in a detection system with high analytical sensitivity. This approach is used in the SeraLyte-TBTM system, a sensitive chemiluminescent test for antibodies that bind to ferrite particles coated with the MPB83 antigen. SeraLyte testing was conducted in blinded fashion on frozen sera (N = 90 samples) collected from experimentally infected and control cattle (N = 71 animals). The sample included sera from animals infected with M. bovis (N = 27), M. kansasii (N = 4), M. avium subspecies paratuberculosis (N = 11), M. avium subspecies avium (N = 12) and uninfected animals (N = 17).  As with all serological tests that utilize MPB83, reactivity was expected in M. bovis and M.
kansasii-infected animals, but not in M-avium-infected and uninfected animals. Upon unblinding, sensitivity relative to known infection was 90% among the 71 individual animals, and 92% among the 90 individual samples tested. Specificity was 100%. The results indicate that SeraLyte-TB(TM) has sufficient sensitivity for detecting infection based on serological response to a single antigen.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov