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Title:   Author  item
                                            HARRIS, LINDA - AGRICULTURE AGRI-FOOD CANitem
                                            SAPARNO, AUDREY - AGRICULTURE AGRI-FOOD CANitem
                                            Alexander, Nancyitem
                                            BLACKWELL, BARBARA - AGRICULTURE AGRI-FOOD CANitem
                                            McCormick, Susanitem
                                            SCHNEIDERMAN, DANIELLE - AGRICULTURE AGRI-FOOD CANitem
                                            Desjardins, Anneitem
                                            TINKER, NICK - AGRICULTURE AGRI-FOOD CANitem
                                            HATTORI, JIRO - AGRICULTURE AGRI-FOOD CANitem
                                            SEIFERT, KEITH - AGRICULTURE AGRI-FOOD CANitem
                                            OUELLET, THERESE - AGRICULTURE AGRI-FOOD CA
  Submitted to: Fungal Genetics Conference Proceedings
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 1/16/2007
                                    Publication  Date: 3/20/2007
                                    Citation: Harris, L., Saparno, A., Alexander, N.J., Blackwell, B., Mc Cormick, S.P., Schneiderman, D., Desjardins, A.E., Tinker, N., Hattori, J., Seifert, K., Ouellet, T. 2007. A novel gene cluster in Fusarium graminearum expressed under mycotoxin induction conditions [abstract]. Fungal Genetics Conference. p. 104.Interpretive Summary:
                                Technical Abstract: We have identified a cluster of eight genes (gene loci fg08077 - fg08084) in Fusarium graminearum that is concomitantly up-regulated (Northern and qPCR analysis) under growth conditions that promote mycotoxin production.  Proteomics experiments (iTRAQ analysis) have confirmed the up-regulation of proteins encoded by two of these genes under toxin induction conditions.  Gene disruption and add-back experiments followed by metabolite analysis of the transformants indicated that one of the genes, fg08079, is directly involved in the synthesis of butenolide, a secondary metabolite derived from glutamic acid.  As expression of these genes can be detected very early during plant infection, butenolide may play a role in plant infection.  However, greenhouse testing for FHB (Fusarium Head Blight) using disruption mutants of fg08079 showed that this gene did not contribute significantly to virulence in wheat heads.  The mycotoxin butenolide is produced by several Fusarium species and has been suggested to be associated with tall fescue toxicoses in grazing cattle.  We are exploring interspecies conservation of this gene cluster by PCR screening and sequencing in other Fusaria genomes.


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