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Title:   Author  item
                                            MEENAKSHI, R - INDIAitem
                                            SANDHU, K - INDIAitem
                                            SINGH, J - INDIAitem
                                            SHARMA, S - INDIAitem
                                            SIDHU, P - INDIAitem
                                            SREEKUMAR, C - USDA ARS ANRI APDLitem
                                            Dubey, Jitender
  Submitted to: Journal of Parasitology
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 8/1/2007
                                    Publication  Date: 12/5/2007
                                    Citation: Meenakshi, R.H., Sandhu, K.S., Singh, J., Sharma, S., Sidhu, P.K., Sreekumar, C., Dubey, J.P. 2007. Neospora caninum infections in cattle in india seroprevalence of neospora caninum antibodies in cattle and water buffaoes in india  Journal of Parasitology. 93:1374-1377.Interpretive Summary: Neospora caninum is a single-celled parasite. It causes abortion in cattle and paralysis in companion animals. It is the most important cause of abortion in dairy cattle. Dogs and coyotes are its definitive hosts and main reservoirs of infection. This parasite is transmitted efficiently from the cow to the calf transplacentally.

Scientists at the Beltsville Agricultural Research Center and an university in India report  seroprevalence of  N. caninum in cattle and buffaloes in India for the first time..The results will be of interest to biologists, parasitologists, and veterinarians
                                Technical Abstract: Neospora caninum is now recognized as a major cause of abortion in cattle worldwide, but there is no report of N. caninum infection in cattle in India. Serum samples from 427 dairy cattle and 32 dairy water buffaloes from 7 organized dairy farms located in Punjab, India, were tested for N. caninum antibodies using a commercial monoclonal antibody based competitive ELISA. Antibodies to N. caninum were found in 35 of 427 cattle from 6 of the 7 farms; 9.6% of cows, 5.1% of heifers, and 5% of calves were seropositive, suggesting postnatal transmission of N. caninum on the farm. Antibodies to N. caninum were found in 16 of 32 buffaloes tested from 2 dairy farms. A total of 64 cattle and 16 buffalo sera already tested by ELISA was also evaluated by an indirect fluorescent antibody test (IFAT) to verify ELISA results. Of the 64 cattle samples, 29 sera were negative by both tests and of the 35 ELISA-positive sera, 12 had IFAT titers of 1:100 or higher (1 had IFAT titer of 100, 2 had IFAT titer of 200, and 9 had IFAT titers of 400 or higher). Of the 16 buffalo sera positive by ELISA 1 had an IFAT titer of 1:400. Thus, antibodies to N. caninum were demonstrated in cattle sera by 2 serologic methods. To our knowledge this is the first report of N. caninum infection in cattle and buffaloes in India


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