U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Crop Production and Pest Control Research: West Lafayette, IN
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area West Lafayette, Indiana Crop Production and Pest Control Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications
- Small Grains Viral Disease Laboratory
- Corn and Sorghum Fungal Disease Laboratory
- Host Plant Resistance to Insects Laboratory
- Wheat/Hessian fly Interactions Laboratory
- Small Grains Fungal Disease Laboratory
- Soybean Quality Improvement Laboratory
- Uniform Soybean Tests, Northern Region

Title:   Author  item
                                            FERREIRA, C - PLANT RES. INT. NETHEitem
                                            VAN DER LEE, T.A.J. - PLANT RES. INT. NETHERitem
                                            ZAPATER, M - PLANT RES. INT. NETHERitem
                                            CARLIER, J - PLANT RES. INT. NETHERitem
                                            Goodwin, Stephen

                                                         - *Steve*item
                                            SOUZA, JR., M - PLANT RES. INT. NETHERitem
                                            KEMA, G.H.J. - PLANT RES. INT. NETHER
  Submitted to: Symposium Proceedings
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 7/26/2008
                                    Publication  Date: 8/18/2008
                                    Citation: Ferreira, C.F., Van Der Lee, T., Zapater, M.F., Carlier, J., Goodwin, S.B., Souza, Jr., M.T., Kema, G. 2008. Development of a genetic linkage map of Mycosphaerella fijiensis, the causal agent of black leaf streak disease in bananas (Musa spp.) using SSR and DArT markers. Symposium Proceedings. Available: http://www.path.ethz.ch/news/conferences/Mycosphaerella_Ascona_2007/008_abs.pdfInterpretive Summary: Mycosphaerella fijiensis is the causal agent of black leaf streak (BLS) disease in bananas. This pathogen threatens global banana production as the main export cultivars are highly susceptible. As a consequence, commercial banana plantations must be protected chemically with fungicides; up to 40 applications are per year representing >40% of total production costs. Ascospores are the major drivers of BLS epidemics and M. fijiensis is currently still colonizing new banana-growing areas, which has a huge economic impact on banana producers. The frequent fungicide applications exert a strong selection pressure on M. fijiensis populations resulting in abrupt resistance development to strobilurins and a gradual increase of resistance to azole fungicides. To provide the community with necessary genetic tools we developed a genetic linkage map of M. fijiensis. One-hundred and thirty-six F1 individuals from the CIRAD086 (Cameroon, Mat1-1) x CIRAD 139A (Colombia, Mat1-2) cross were evaluated. Eighty-seven Simple-Sequence Repeat (SSR) markers, 3 Variable Number of Tandem Repeat (VNTR) markers, the mating type locus and 235 Diversity Arrays Technology (DArT) markers were positioned in 19 linkage groups covering 1417 cM of the genome. The arrays containing individual fragments of the M. fijiensis genome generated DArT markers with a 90% genotype call rate and 98.8% reliability score. In total, 87% of the markers could be positioned reliably with LOD scores <10. The map is the first high-quality genetic linkage map of M. fijiensis with SSR and Dart markers.  Due to its excellent genome coverage we decided to sequence the DArT markers to align this genetic map with the genome sequence of CIRAD086, which will considerably assist the genome assembly of this important fungus.
                                Technical Abstract: Mycosphaerella fijiensis is the causal agent of black leaf streak (BLS) disease in bananas. This pathogen threatens global banana production as the main export cultivars are highly susceptible. As a consequence, commercial banana plantations must be protected chemically with fungicides; up to 40 applications are per year representing >40% of total production costs. Ascospores are the major drivers of BLS epidemics and M. fijiensis is currently still colonizing new banana-growing areas, which has a huge economic impact on banana producers. The frequent fungicide applications exert a strong selection pressure on M. fijiensis populations resulting in abrupt resistance development to strobilurins and a gradual increase of resistance to azole fungicides. To provide the community with necessary genetic tools we developed a genetic linkage map of M. fijiensis. One-hundred and thirty-six F1 individuals from the CIRAD086 (Cameroon, Mat1-1) x CIRAD 139A (Colombia, Mat1-2) cross were evaluated. Eighty-seven Simple-Sequence Repeat (SSR) markers, 3 Variable Number of Tandem Repeat (VNTR) markers, the mating type locus and 235 Diversity Arrays Technology (DArT) markers were positioned in 19 linkage groups covering 1417 cM of the genome. The arrays containing individual fragments of the M. fijiensis genome generated DArT markers with a 90% genotype call rate and 98.8% reliability score. In total, 87% of the markers could be positioned reliably with LOD scores <10. The map is the first high-quality genetic linkage map of M. fijiensis with SSR and Dart markers.  Due to its excellent genome coverage we decided to sequence the DArT markers to align this genetic map with the genome sequence of CIRAD086, which will considerably assist the genome assembly of this important fungus.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov