U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Crops Pathology and Genetics Research: Davis, CA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Outreach Events
                        UC Davis Picnic Day
                                    Kinders go to College
                                    STEM Girls
                                    Future Scientist Program
                                    Agriculture Future of America
                                    Wallace-Carver Fellowship
                                    Young Scholars Program
                                    Annual Awards Banquet
                    About Us
                        About Us
                                    Our Mission
                                    Principal InvestigatorsSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Pacific West Area Davis, California Crops Pathology and Genetics Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications
- Etiology and Biology of Tree Crop Diseases
- Rice Genetics
- Sustainable Floriculture
- Sustainable Viticulture

Title:   Author  item
                                            XU, XINJIA - DEPT PLANT SCI, UC DAVISitem
                                            Jiang, Cai-Zhongitem
                                            Donnelly, Lindaitem
                                            REID, MICHAEL - DEPT PLANT SCI, UC DAVIS
  Submitted to: Journal of Experimental Botany
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 7/30/2007
                                    Publication  Date: 10/1/2007
                                    Citation: Xu, X., Jiang, C., Donnelly, L.M., Reid, M.S. Functional analysis of a ring domain ankyrin repeat protein that is highly expressed during flower senescence. Journal of Experimental Botany.Interpretive Summary:
                                Technical Abstract: A gene encoding a RING zinc finger ankyrin repeat protein (MjXB3), a putative E3 ubiquitin ligase, is highly expressed in petals of senescing four o'clock (Mirabilis jalapa) flowers, increasing >40 000-fold during the onset of visible senescence.  The gene has homologues in many other species, and the Petunia homologue is strongly up-regulated in senescing Petunia corollas.  Silencing the expression of this gene in Petunia, using virus-induced gene silencing, resulted in a 2 d extension in flower life.  In Mirabilis, a 2 kb promoter region, 5' upstream of the MjXB3 gene, was isolated.  The promoter sequence included putative binding sites for many DNA-binding proteins, including the bZIP, Myb, homeodomain-leucine zipper (HD-Zip), MADS-box, and WRKY transcription factors.  The construct containing a 1 kb promoter region immediately upstream of the MjXB3 gene drove the strongest expression of the b-glucuronidase (GUS) reporter gene in a transient expression assay.  In Petunia, GUS expression under the control of this heterologous promoter fragment was specific to senescing flowers.  The Mirabilis promoter GUS construct was tested in other flower species; while GUS activity in carnation petals was high during senescence, no expression was detected in three monocotyledonous flowers-daylily (Hemerocallis 'Stella d'Oro'), daffodil (Narcissus pseudonarcissus 'King Alfred'), and orchid (Dendrobium 'Emma White').


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov