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Title:   Author  item
                                            KEMA, GERT H - WAGENINGEN, NETHERLANDSitem
                                            Dunkle, Larryitem
                                            CHURCHILL, ALICE C - CORNELL UNIV.item
                                            CARLIER, JEAN - CIRAD, FRANCEitem
                                            JAMES, ANDY - CICY, MEXICOitem
                                            SOUZA JR., MANOEL - EMBRAPA, BRAZILitem
                                            CROUS, PEDRO - FUNGAL CENTRE, NETHERLANDitem
                                            ROUX, NICOLAS - INIBAP, FRANCEitem
                                            VAN DER LEE, THEO A - WAGENINGEN, NETHERLANDSitem
                                            WITTENBERG, ALEXANDER - WAGENINGEN, NETHERLANDSitem
                                            LINDQUIST, ERIKA - DOE, WALNUT CREEK, CAitem
                                            GRIGORIEV, IGOR - DOE, WALNUT CREEK, CAitem
                                            BRISTOW, JIM - DOE, WALNUT CREEK, CAitem
                                            Goodwin, Stephen

                                                         - *Steve*
  Submitted to: Plant and Animal Genome VX Conference Abstracts
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 12/1/2006
                                    Publication  Date: 1/13/2007
                                    Citation: Kema, G.J., Dunkle, L.D., Churchill, A.L., Carlier, J., James, A., Souza Jr., M.T., Crous, P., Roux, N., Van Der Lee, T.J., Wittenberg, A., Lindquist, E., Grigoriev, I., Bristow, J., Goodwin, K.R. Sequencing the Major Mycosphaerella Pathogens of Wheat and Banana [abstract]. Plant and Animal Genome VX Conference. Abstract No. W162.Interpretive Summary:
                                Technical Abstract: Mycosphaerella is one of the largest genera of plant pathogenic fungi with more than 1,000 named species, many of which are important pathogens causing leaf spotting diseases in a wide variety of crops including cereals, citrus, banana, eucalypts, soft fruits, and horticultural crops. A few species of Mycosphaerella cause disease in humans and other vertebrates. An international project was initiated to sequence the genomes of M. graminicola and M. fijiensis, two of the most economically important pathogens of wheat and banana, respectively, along with 40,000 ESTs from M. fijiensis and the related maize pathogen Cercospora zeae-maydis, through the Community Sequencing Program sponsored by the U.S. DOE-Joint Genome Institute. The 9' M. graminicola sequencing is complete and was made public November 1, 2006 following automated and manual annotation. Due to the very good assembly statistics as well as a >2000-marker DArT linkage map that was aligned to the genome, JGI decided to finish the M. graminicola genome at the Stanford Human Genome Center. The majority of chromosomes have been sequenced completely including both telomeres. These data indicate that M. graminicola has both the largest chromosome number and the smallest chromosome sizes recorded among filamentous ascomycetes. The M. fijiensis EST sequencing has resulted in more than 30,000 ESTs and the genome sequencing is currently at approximately 2.5', which enabled us to revise the genome size estimate of M. fijiensis to approximately 68 Mb, which is 70% larger than that for M. graminicola. The current status of both sequencing projects will be discussed.


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