U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Immunity and Disease Prevention Research: Davis, CA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Laboratories
                        Stephensen Group
                                    Kable Group
                                    Lemay GroupSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Pacific West Area Davis, California Western Human Nutrition Research Center Immunity and Disease Prevention Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            YU, YAN - UC DAVIS, DEPT. OF NUTR.item
                                            Kirschke, Catherineitem
                                            Huang, Liping
  Submitted to: Journal of Histochemistry and Cytochemistry
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 10/27/2006
                                    Publication  Date: 10/27/2006
                                    Citation: Yu, Y.Y., Kirschke, C.P., Huang, L. 2006. IMMUNOHISTOCHEMICAL ANALYSIS OF ZNT1, 4, 5, 6, AND 7 IN THE MOUSE GASTROINTESTINAL TRACT. Journal of Histochemistry and Cytochemistry.  Vol. 55(3):223-234, 2006.Interpretive Summary: Zinc is an essential trace metal for mammals. Carries or transporters are needed for zinc to be absorbed from the gut, distributed throughout the body, and excreted from the body. We examined the presence of five zinc transporter proteins (ZnT1, 4, 5, 6, and 7) in the mouse digestive system. The small intestine is the major site for digestion of food and for absorption of the digested products. It can be divided into three segments (duodenum, jejunum, and ileum) based on their anatomic appearances. The duodenum is the part of the small intestine extending from the stomach. The jejunum is the middle part of the small intestine. The ileum is 1/3 part of small intestine adjacent to the large intestine. The large intestine is composed of the cecum, colon, rectum, and anal canal. Its principal functions are to receive the undigested food from the small intestine, reabsorption of electrolytes, metal, and water and elimination of undigested food. Our study indicated that the amount of each ZnT protein in the mouse gut were different. The localization of each ZnT protein was unique but with some overlapping. ZnT1 was detected a lot in the absorptive cells in the esophagus, duodenum of the small intestine, and cecum of the large intestine. ZnT4 was predominantly detected in the large intestine. ZnT5 was mainly detected in the parietal cell (the cell that secrets acid) of the stomach and in the absorptive cells in the duodenum and jejunum. ZnT6 was predominantly detected in the chief cell (the cell that secrets digestive enzymes) of the stomach, absorptive cells in the jejunum, cecum, colon, and rectum. Lastly, ZnT7 was observed in absorptive cells along the mouse digestive system. The high levels of ZnT proteins detected in the absorptive cells in the digestive system suggest that ZnT proteins may play important roles in zinc absorption and zinc secretion in the gut.
                                Technical Abstract: Expression of five zinc transporters (ZnT1, 4, 5, 6, and 7) of the Slc30 family in the mouse gastrointestinal tract was studied by immunohistochemical analysis. The results demonstrated unique expression patterns, levels, and cellular localization among ZnT proteins in the mouse gastrointestinal tract with some overlapping. ZnT1 was abundantly expressed in the epithelium of the esophagus, duodenum of the small intestine, and cecum of the large intestine. ZnT4 was predominantly detected in the large intestine. ZnT5 was mainly expressed in the parietal cell of the stomach and in the absorptive epithelium of the duodenum and jejunum. ZnT6 was predominantly detected in the chief cell of the stomach, columnar epithelial cells of the jejunum, cecum, colon, and rectum. Lastly, ZnT7 was observed in all epithelia of the mouse gastrointestinal tract with the highest expression in the small intestine. The abundant expression of ZnT proteins in the absorptive epithelial cell of the gastrointestinal tract suggests that ZnT proteins may play important roles in zinc absorption and endogenous zinc secretion.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov