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Title:   Author  item
                                            Freking, Bradley

                                                         - *Brad*item
                                            Miles, Jeremyitem
                                            BISCHOFF, STEVEN - NORTH CAROLINA STATE UNIVitem
                                            XIA, YUANNAN - UNIVERSITY OF NEBRASKAitem
                                            Nonneman, Danny

                                                         - *Dan*item
                                            Vallet, Jeffitem
                                            PIEDRAHITA, JORGE - NORTH CAROLINA STATE UNIV
  Submitted to: Midwestern Section of the American Society of Animal Science
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 11/16/2007
                                    Publication  Date: 9/22/2008
                                    Citation: Freking, B.A., Miles, J.R., Bischoff, S.R., Xia, Y., Nonneman, D.J., Vallet, J.L., Piedrahita, J.A. 2008. Placental transcriptome profile differences associated with selection for uterine capacity [abstract]. Journal of Animal Science. 86(E-Suppl. 3):49. Abstract #36.Interpretive Summary:
                                Technical Abstract: Selection for 11 generations for uterine capacity (UC) resulted in 1.6 more live pigs born with no change in birth and placental weights.  It was determined that the critical time period for the difference in litter size was established between d 25 and 45 of gestation.  Our objective was to gain insight into placental transcriptional changes after selection for uterine capacity.  Thirty gilts from the UC and control (CO) lines were subjected to unilateral hysterectomy-ovariectomy at approximately 160 d of age and mated within line at approximately 280 d.  Gilts were slaughtered at d 25, 30, or 40 of gestation.  Fetal and placental tissues were obtained from each live embryo.  Two male and two female embryos closest to the litter mean for placental weight were chosen to represent each litter (n = 3 litters per line and time point combination).  Placental tissues were pooled within litter and total RNA was extracted.  Samples were labeled and hybridized to Affymetrix porcine array chips (n = 18) using manufacturers suggested protocols.  Signal intensities were normalized using GC content Robust Multi-array Average (GCRMA) on the probe level data.  Two-way ANOVA (two lines and three stages) was performed.  Threshold values were set at a minimum of 1.5 fold difference and the false discovery rate was set to P < 0.05 (Benjamini and Hochberg algorithm).  A total of 4171 targets on the array exceeded P value and threshold limits for the main effect of stage (2230 up-regulated and 1839 down-regulated from d 25 to d 40).  Two targets, LIM domain and actin-binding protein 1 (LIMA1) and dedicator of cytokinesis 4 (DOCK4) approached significance for line (P < 0.08).  Both genes appear to play roles in cell migration, suggesting participation in placental folded bilayer formation.


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