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Title:   Author  item
                                            MUZYCHKINA, L.K. - INST. PLT BIO & BIOTECHitem
                                            KORULKIN, D.J. - INST. PLT BIO & BIOTECHitem
                                            ZAVADSKIY, V.A. - INST. PLT BIO & BIOTECHitem
                                            Cantrell, Charles
  Submitted to: Book Chapter
                                    Publication Type: Book / Chapter
                                    Publication Acceptance Date: 4/15/2008
                                    Publication  Date: 4/15/2008
                                    Citation: Muzychkina, L., Korulkin, D., Zavadskiy, V., Cantrell, C.L. 2008. Extraction, Separation, Identification and Quantity Analysis of Plant Substances. Book Chapter. Vassilyev, J., Gemedjiyeva, N., Sitpaeva, G., Cantrell, C.L., Mamonov, L. 2008. Preparation of Plant Samples for Phytochemical Research and the Study of Their Biological Activities. Book Chapter. In: Introduction to Phytochemical Investigations and Discovering Biological Activities of Phytogenous Substances. Eds. B.M. Butin and B.A. Sarsenbaev. Institute of Plant Biology and Biotechnology, Almaty, Kazakhstan. pp. 87-126.Interpretive Summary: Phytochemical investigations often begin with a series of crude extractions consisting of a series of solvents progressing from non-polar to polar.  The crude extracts are further separated using liquid/liquid chromatography techniques to enable broad separations based on liquid solubility.  Finally a series of chromatographic column purifications are chosen based on the origin fraction solubilities and thin layer chromatography results. This is usually a combination of both normal and reverse phase silica gel chromatography; however, a number of different solid phases are available.  Identification of isolated bioactive constituents typically involves a combination of high resolution mass spectrometry (HRMS) and nuclear magnetic resonance (NMR) spectroscopic techniques.  For novel compounds, NMR techniques almost always involve both one- and two-dimensional NMR techniques.
                                Technical Abstract: Phytochemical investigations often begin with a series of crude extractions consisting of a series of solvents progressing from non-polar to polar.  The crude extracts are further separated using liquid/liquid chromatography techniques to enable broad separations based on liquid solubility.  Finally a series of chromatographic column purifications are chosen based on the origin fraction solubilities and thin layer chromatography results. This is usually a combination of both normal and reverse phase silica gel chromatography; however, a number of different solid phases are available.  Identification of isolated bioactive constituents typically involves a combination of high resolution mass spectrometry (HRMS) and nuclear magnetic resonance (NMR) spectroscopic techniques.  For novel compounds, NMR techniques almost always involve both one- and two-dimensional NMR techniques.


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