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Title:   Author  item
                                            Yu, Qingzhongitem
                                            Estevez, Carlositem
                                            Kapczynski, Darrell
  Submitted to: American Association of Avian Pathologists
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 1/17/2006
                                    Publication  Date: 7/18/2006
                                    Citation: Yu, Q., Estevez, C., Kapczynski, D.R. 2006. Production and characterization of monoclonal antibodies produced against Avian Metapneumovirus Subtype C which react against the nucleocapsid protein [abstract].  In: Proceedings of the 2006 Annual Meeting of American Association of Avian Pathologists, July 16-19, 2006, Honolulu, HI. p. 164.Interpretive Summary:
                                Technical Abstract: Monoclonal antibodies (MAbs) were prepared against avian metapneumovirus (aMPV) subtype C virus (aMPV/Minnesota/turkey/1a/97). Six MAbs were selected based on ELISA activities and characterized by isotyping, neutralization test, Western blot analysis, and immunohistochemistry assay. The results showed all six MAbs reacted with the nucleocapsid (N) protein of aMPV, but did not neutralize aMPV infectivity at a detectable level. Three MAbs (3E, 9D and 12C) belonged to IgG1 subclass, whereas the other three (5D, 8E and 16E) were related to the IgG2a subclass. These MAbs provide new tools and methods for investigating aMPV infection, pathogenesis, and diagnosis of aMPV disease.


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