U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 close



Research
                Research Home
                            National Programs
                            Research Projects
                            Scientific Manuscripts
                            International Engagement
                            Scientific Software/Models
                            Databases and Datasets
                            Office of Scientific Quality Review
            Media
                News & Features
                            Multimedia
                            Briefing Room
                            Down on the Farm
                            Press Room
                            Science in Your Shopping Cart
                            Scientific Discoveries
                            Tellus
                            Under the Microscope
            About ARS
                About ARS Home
                            Staff Directory
                            Labs and Research Centers (Map)
                            Headquarter Offices
                            Organizational Chart
                            Employee Services (REE Employees Only)
                            Office of Outreach, Diversity, and Equal Opportunity
            Work With Us
                Work With Us Home
                            ARS Vacancies at USAJOBS
                            Careers at ARS Information
                            Scientific Collaborations
                            Extramural Agreements
                            1890 Faculty Research Sabbatical ProgramSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Northeast Area Beltsville, Maryland (BHNRC) Beltsville Human Nutrition Research Center Food Components and Health Laboratory Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Carkeet, Colleenitem
                                            Clevidence, Beverlyitem
                                            Novotny, Janet
  Submitted to: Federation of American Societies for Experimental Biology Conference
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 1/10/2006
                                    Publication  Date: 4/1/2006
                                    Citation: Carkeet, C., Clevidence, B.A., Novotny Dura, J. 2006. Stability of urinary metabolites of strawberry anthocyanins. Federation of American Societies for Experimental Biology Conference.Interpretive Summary: n/a
                                Technical Abstract: The stability of urinary metabolites of strawberry anthocyanins was investigated.  Healthy adult volunteers consumed 200g of pureed strawberries as a bolus dose and urine was collected and pooled.  Urine was acidified with 1/5 the volume of 0.44M TFA, and malvidin-3-galactose was added as an internal standard.  Urine was separated into two fractions, one for immediate prep and analysis and one frozen at -80 degrees C for 48 hrs before work-up.  Sample prep consisted of solid phase (C18) extraction to isolate and concentrate the anthocyanins.  Anthocyanin metabolites were then separated and detected on an Agilent 1100 HPLC equipped with a diode array detector and a reverse-phase column.  The impact of freezing on anthocyanin metabolite profiles was evaluated and results indicated that freezing had no effect on the peak areas of the four anthocyanin metabolites detected.  An additional experiment followed the change in these metabolite profiles at hourly intervals as the samples remained on a cooled, 10 degrees C autosampler for 18 hours.  These results suggest that three metabolites degrade over time while one peak area increased over time, indicating it may be a degradation product of the other metabolites.  A final experiment investigated the effect of autosampler temperature (10, 20, and 30 degrees C) on the metabolite profiles and revealed a positive correlation between degradation rate and autosampler temperature.  Identification of the four metabolites is underway.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov