U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Food and Feed Safety Research: College Station, TX
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Plains Area College Station, Texas Southern Plains Agricultural Research Center Food and Feed Safety Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Ramlachan, Nicoleitem
                                            Poole, Toniitem
                                            Edrington, Thomasitem
                                            Anderson, Robinitem
                                            Nisbet, David
  Submitted to: American Society for Microbiology
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 7/5/2006
                                    Publication  Date: 9/9/2006
                                    Citation: Ramlachan, N., Poole, T.L., Edrington, T.S., Anderson, R.C., Nisbet, D.J. 2006. Genetic characterization and correlation to multidrug resistance in isolates of Salmonella enterica serovar Newport derived from beef and dairy cattle [abstract]. American Society for Microbiology. p. 88-89.Interpretive Summary:
                                Technical Abstract: High-throughput genotyping of Salmonella isolates obtained from food production animals is an essential component of food safety to evaluate pathogenic and antimicrobial resistance profiles.  A total of 99 strains of Salmonella enterica serovar Newport were studied by pulsed-field gel electrophoresis (PFGE) to elucidate genetic relationships among strains isolated from beef and dairy cattle slaughterhouse hide and carcass samples.  Antibiotic susceptibilities were determined and interpreted according to MIC interpretive standards for antibiotics including: amoxicillin/clavulanate, ampicillin, cefoxitin, ceftiofur, cephalothin, chloramphenicol, kanamycin, streptomycin, sulfamethoxazole, tetracycline and ceftriaxone.  Some isolates were found to be resistant to 10 or more antibiotics. PFGE of XbaI-digested chromosomal DNA and plasmid DNA fingerprinting profiles were used to assign Salmonella isolates into clonal groups and to determine plasmid content on the basis of size.  Cluster analysis based on the unweighted pair group method using an arithmetic averages algorithm identified three main clusters independent of plasmid content, indicating that the divergence of the PFGE subtypes was probably derived from genomic mutations.  The majority of pan-susceptible isolates grouped in the same cluster had similar XbaI profiles.  The data confirms that strains isolated from beef or dairy cattle were of a unique clonal origin and that a particular PFGE combination profile was predominant and specific for pan-susceptible isolates from beef and dairy cattle.  Correlations between antimicrobial resistance phenotype and genotype, as well as predicted pathogenicity and presence of predisposing conjugative elements and competitive fitness for each isolate tested revealed further differentiation within beef or dairy groups.  Combined phenotypic and genotypic profiles are necessary for epidemiological studies and public health investigations on S. Newport.  The use of such methodologies for genetically determining multidrug resistance status, pathogenicity and ability to transfer and/or uptake such genes in a Salmonella spp. of public health concern, will allow us to elucidate the mechanisms by which S. Newport increases in pathogenicity causing fatal disease in cattle as well as serious cases of human food poisoning.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov