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Title:   Author  item
                                            McCormick, Susanitem
                                            Appell, Michaelitem
                                            Alexander, Nancyitem
                                            Desjardins, Anne
  Submitted to: American Chemical Society National Meeting
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 12/20/2007
                                    Publication  Date: 4/10/2008
                                    Citation: Mc Cormick, S.P., Appell, M.D., Alexander, N.J., Desjardins, A.E. 2008. Phytotoxicity of trichothecenes using Arabidopsis detached leaf and Chlamydomonas assays [abstract]. American Chemical Society. Available: http://www.acs.org  Paper #AGFD #145.Interpretive Summary:
                                Technical Abstract: Many plant pathogenic species of the genus Fusarium produce trichothecenes, a large group of sesquiterpene epoxides that are inhibitors of eukaryotic protein synthesis.  Fusarium graminearum, F. sporotrichioides, and other trichothecene-producing Fusarium species are common pathogens of cereal grains, especially in temperate climates in Europe, Asia, and the Americas, where trichothecene contamination of wheat, barley, rye, and maize can be a serious problem.  Although some Fusarium trichothecenes are virulence factors in plant disease, the phytotoxicities of many trichothecenes have only recently been investigated.  We used two test systems to determine which structural features might influence phytotoxicity; namely, detached leaves of Arabidopsis thaliana plants and the unicellular plant Chlamydomonas reinhardtii.  Each was treated with solutions of natural and synthetically modified trichothecenes that differ in oxygenation and acetylation.  Results of these studies suggest that trichothecenes with a C-3 acetyl group are generally less phytotoxic that those with a C-3 hydroxyl group.  The results also indicated that other structural features may be important.


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