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ARS Home Midwest Area Ames, Iowa National Animal Disease Center Virus and Prion Research Research Publications at this Location





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Title:   Author  item
                                            Hamir, Amiraliitem
                                            CUTLIP, RANDALL - ARS RETIREDitem
                                            MILLER, JANICE - ARS RETIREDitem
                                            Kunkle, Robertitem
                                            Greenlee, Justinitem
                                            Richt, Juergen
  Submitted to: American Association of Veterinary Laboratory Diagnosticians
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 6/3/2005
                                    Publication  Date: 11/2/2005
                                    Citation: Hamir, A.N., Cutlip, R.C., Miller, J.M., Kunkle, R.A., Greenlee, J.J., Richt, J.A. 2005. Experimental transmission of transmissible spongiform encephalopathies (TSE) at the National Animal Disease Center, Ames, Iowa: an update [abstract]. American Association of Veterinary Laboratory Diagnosticians. p. 169.Interpretive Summary:
                                Technical Abstract: Experimental transmission of TSE agents provides valuable information for identification of potential host ranges, and generates much needed prion-infected tissues for research. Such investigations have been conducted at NADC since 1990 and up-to-now have been confined to scrapie, chronic wasting disease (CWD), and transmissible mink encephalopathy (TME). Most of these cross-species transmission studies require long incubation periods and need BL-2 conditions for conducting the experiments. Initially our studies were restricted to farm livestock (cattle and sheep). However, as a result of increased demand from our stakeholders, we now also conduct research on wildlife (herbivores and carnivores). Following are some of the significant findings of past experiments at NADC: 
-Intracerebral inoculation of sheep scrapie into cattle resulted in a neurologic disease in that was distinct from bovine spongiform encephalopathy (BSE). 

-Oral inoculation of cattle with scrapie infected brain did not result in clinical disease during eight years of observation.

-Sheep scrapie was transmissible to elk by intracerebral route and the resulting pathology could not be distinguished from CWD in elk.

-Intracerebral inoculation of CWD was transmissible to 38% of cattle and 12.5% sheep inoculated with CWD from mule deer.

-TME agent was transmissible to cattle by the intracerebral route and the resulting pathology could not be distinguished from published lesions of BSE. 

-TME and scrapie agents were transmissible to raccoons via intracerebral route, whereas CWD from mule deer was not. 

-Genetically susceptible sheep inoculated with scrapie by intracerebral and oral routes developed clinical disease within 19 and 32 months, respectively. Resistant sheep have not developed scrapie 30 months post inoculation. 

-Immunohistochemistry method utilized at this laboratory did not reveal prion in muscle tissues of animals (cattle, sheep, elk, raccoons) infected with TME, scrapie or CWD.


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