U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Virus and Prion Research: Ames, IA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area Ames, Iowa National Animal Disease Center Virus and Prion Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            LEKCHAROENSUK, PORNTIPPA - KASETSART UNIV, BANGKOKitem
                                            Baker, Amyitem
                                            Lager, Kellyitem
                                            SOLORZANO, ALICIA - MOUNT SINAI SCHOOL OF MEDitem
                                            GARCIA-SASTRE, ADOLFO - MOUNT SINAI SCHOOL OF MEDitem
                                            Richt, Juergen
  Submitted to: International Union of Microbiological Societies Proceedings/Abstracts
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 6/1/2005
                                    Publication  Date: 7/23/2005
                                    Citation: Lekcharoensuk, P., Vincent, A.L., Lager, K.M., Solorzano, A., Garcia-Sastre, A., Richt, J.A. 2005. Establishment of a pig model for the 1930 H1N1 swine influenza virus and application of reverse genetics [abstract]. International Congress of Virology. p. 42.Interpretive Summary:
                                Technical Abstract: The aims of this study were to establish a pig model for the 1930 H1N1 swine influenza virus and its rescue by reverse genetics. Influenza A/swine/IA/15/30 H1N1 (ATCC VR333) is the first influenza virus isolated from swine and genetically related to the 1918 Spanish Flu virus.  We inoculated thirty 4-week-old pigs with the H1N1 SIV.  Pigs were euthanized at 3, 5 and 7 dpi.  Average macroscopic lung lesion scores were 9%, 13% and 11% on 3, 5, and 7 dpi, respectively.  Virus titers in the bronchioalveolar lavage were 10**4, 10**3.8 and 10**1.1 TCID50/ml at 3, 5 and 7 dpi, respectively.  Virus shedding in nasal secretions at dpi 3 and 5 were significantly higher than those at 7 dpi.  In vitro interferon beta bioassay showed that the 1930 SIV was able to inhibit type I interferon production, similar to other pathogenic influenza viruses. The sequence from the pig-passaged virus was compared to the ATCC VR333 GenBank reference sequence: HA, NA and NS genes were identical, PB2 had 99.9% homology with 2 nucleotide mutations, PA had 99.9% homology with one mutation resulting in a serine to cysteine substitution; one silent mutation was found in the NP and M genes.  No PB1 reference sequence was found for comparison.  We are now constructing an infectious clone using reverse genetics. In summary, we have established a pig model for studies on the pathogenicity of the 1930 H1N1 SIV and we are now constructing an infectious clone using reverse genetics. Since pigs are susceptible to swine, avian and human influenza viruses, a pig model in combination with the reverse genetics system could be a valuable tool for studying the molecular basis of virulence and genetic reassortment.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov