U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Global Change and Photosynthesis Research: Urbana, IL
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area Urbana, Illinois Global Change and Photosynthesis Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            GILLESPIE, KELLY - UNIVERSITY OF ILLINOISitem
                                            Chae, Juneitem
                                            Ainsworth, Elizabeth

                                                         - *Lisa*
  Submitted to: Nature Protocols
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 2/9/2007
                                    Publication  Date: 4/12/2007
                                    Citation: Gillespie, K.M., Chae, J.M., Ainsworth, E.A. 2007. Rapid Measurement of Total Antioxidant Capacity in Plants. Nature Protocols.  2:867-870.Interpretive Summary: There is growing interest in measuring the antioxidant status of plant tissues, in particular in response to stress.  This article describes the oxygen radical absorbance capacity (ORAC) assay for measuring the total antioxidant capacity in plant tissue extracts.  The high-throughput assay is performed in a microplate and is assessed with a 96-well multi-detection plate reader.  This assay is presented along with rapid assays for total phenolic content and total ascorbate content.  Collectively, these assays provide scientists and researchers with general diagnostic tools of the antioxidant capacity of leaf tissue extracts.
                                Technical Abstract: There is growing interest in measuring the antioxidant status of plant tissues.  This article describes the oxygen radical absorbance capacity (ORAC) assay, which measures antioxidant inhibition of peroxyl radical-induced oxidations and is a measure of total antioxidant capacity.  The assay is performed in a microplate and is assessed with a 96-well multi-detection plate reader.  With the described protocol, total antioxidant capacity of 64 experimental samples can easily be analyzed in one day.  This assay is presented along with rapid assays for total phenolic content and total ascorbate content.  Collectively, these assays provide a general diagnostic tool of the antioxidant capacity of leaf tissue extracts.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov