U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Corn Insects and Crop Genetics Research: Ames, IA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Database Projects
                        MaizeGDB
                                    SoyBase
                                    PeanutBase
                                    Legume Information System
                                    Maize Newsletter
                                    Maize Mutant Phenotypes
                                    Maize Genetics Conference
                                    Shiny Soybase CNV
                                    Shiny Soybase SNP
                                    Common Bean and Alfalfa - PvGEA
                                    Common Bean and Alfalfa - AGEDSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area Ames, Iowa Corn Insects and Crop Genetics Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            MOSCOU, M - IOWA STATE UNIVERSITYitem
                                            Lauter, Nicholasitem
                                            CALDO, R - IOWA STATE UNIVERSITYitem
                                            Wise, Roger
  Submitted to: Annual International Plant & Animal Genome Conference
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 12/7/2007
                                    Publication  Date: 12/7/2007
                                    Citation: Moscou, M.J., Lauter, N.C., Caldo, R.A., Wise, R.P. 2007. Gene-For-Gene-Mediated Transcriptome Reprogramming in Barley-Powdery Mildew Interactions [abstract]. Annual International Plant & Animal Genome Conference. Abstract Book. p. 88.Interpretive Summary:
                                Technical Abstract: Barley has a complex interaction with powdery mildew [Blumeria graminis f. sp. hordei (Bgh)] that begins with early recognition of microbe-associated molecular patterns (MAMPs) from the pathogen. During Bgh invasion of the epidermis, the fate of cells is decided by the presence of resistance (R) genes that mediate an immediate response, which halts the progress of the pathogen. To understand the regulatory role and response associated with R-gene-mediated defense, we surveyed the transcriptional response of barley upon pathogen inoculation using three Manchuria NILs carrying allelic variants at the Mla locus, null mutations mla1-m508 and mla6-m9472, as well as Sultan 5 (Mla12) and mutants derived there from, mla12-m66, rar-1-1, rar1-2, and rom1. Each experiment consisted of sampling at 0, 8, 16, 20, 24, and 32 hours after inoculation with three replications, varying only in the inclusion of non-inoculated material. We found that the resistance response manifests itself via dynamic reprogramming of the transcriptome, which includes over 5,000 genes and may likely exceed 10,000. In addition, the same genes that are coordinately expressed in resistant versus susceptible reactions are differentially expressed between inoculated and non-inoculated tissue. By contrast, the quantitative nature of the Mla control becomes apparent when we observe null mutations, where early signaling effects are compromised by this gene loss. Collectively, our results confirm the high-level regulatory control of Mla in gene-for-gene-mediated resistance and point to an essential early regulatory role as observed via massive transcriptome reprogramming.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov