U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 close



Research
                Research Home
                            National Programs
                            Research Projects
                            Scientific Manuscripts
                            International Engagement
                            Scientific Software/Models
                            Databases and Datasets
                            Office of Scientific Quality Review
            Media
                News & Features
                            Multimedia
                            Briefing Room
                            Down on the Farm
                            Press Room
                            Science in Your Shopping Cart
                            Scientific Discoveries
                            Tellus
                            Under the Microscope
            About ARS
                About ARS Home
                            Staff Directory
                            Labs and Research Centers (Map)
                            Headquarter Offices
                            Organizational Chart
                            Employee Services (REE Employees Only)
                            Office of Outreach, Diversity, and Equal Opportunity
            Work With Us
                Work With Us Home
                            ARS Vacancies at USAJOBS
                            Careers at ARS Information
                            Scientific Collaborations
                            Extramural Agreements
                            1890 Faculty Research Sabbatical ProgramSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Tuo, Wenbinitem
                                            Fetterer, Raymonditem
                                            Jenkins, Markitem
                                            Dubey, Jitender
  Submitted to: American Association of Veterinary Parasitologists
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 3/19/2004
                                    Publication  Date: 7/24/2004
                                    Citation: Tuo, W., Fetterer, R.H., Jenkins, M.C., Dubey, J.P. 2004. Functional characterization of hplc-fractionated neospora caninum proteins. American Association of Veterinary Parasitologists p. 123.Interpretive Summary:
                                Technical Abstract: Neospora caninum is an apicoplexan parasite that causes abortion in cattle.  The objective of this study was to identify immunodominant T helper cell antigens (Ag) that may be used as vaccine candidates.  N. caninum-specific T helper cell lines were established from peripheral blood lymphocytes of infected Holstein cows and used to define antigenicity of the N. caninum proteins fractionated by anion exchange chromatography using HPLC.  The results showed that proteins in HPLC Fractions l through 3 had low or undetectable T cell-stimulating activity, whereas proteins in Fractions 4 through 6 had high stimulator activity  Fraction 5 contained the highest antigenic activity detected by all T cell lines.  Western blod analysis showed that Fraction 5 contained 8-10 distinctive N. caninum protein bands recognized by the rabbit and bovine antisera against N caninum tachyzoites (NC-1 stain).  These results suggest that N. caninum proteins that highly stimulated T helper cell proliferatio and cytokine production may be potential candidates of a N. caninum vaccine.  Research in progress includes purification ad identification of the T cell stimulatng N. caninum proteins using proteomics technologies.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov