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Title:   Author  item
                                            Johnson, Ericitem
                                            Berhow, Markitem
                                            Dowd, Patrick
  Submitted to: American Chemical Society Abstracts
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 3/29/2007
                                    Publication  Date: 3/25/2007
                                    Citation: Johnson, E.T., Berhow, M.A., Dowd, P.F. 2006. Transgenic phenolic production in corn silks moderately enhances insect resistance [abstract]. American Chemical Society Abstracts. Paper No. AGFD 155.Interpretive Summary:
                                Technical Abstract: Some phenolic compounds produced in corn silks, such as maysin, can promote resistance to caterpillar pests.  We evaluated transgenic maize engineered to express a maize cDNA controlled by a putative silk specific promoter for secondary metabolite production and corn earworm resistance.  Transgene expression did not enhance silk color, but 56% of newly emerged silks and 57% of mature silks displayed browning when cut, which indicated the presence of P1-produced secondary metabolites that are substrates of silk peroxidases.  Maysin levels were highest in newly emerged browning silks and declined as the tissue matured.  Insect resistance of transgenic silks, regardless of a visible browning reaction, was highest at emergence and declined with maturity.  In addition, mean survivor weights of corn earworm larvae fed mature browning silks were significantly lower than weights of those fed mature non-browning silks.  Some transgenic kernels browned upon drying, suggesting the promoter may not be silk specific.


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