# Patent

## Title
Peptide-based tooth whitening reagents

## Abstract

Peptide-based reagents comprising at least one tooth surface-binding peptide for delivery of at least one polymer-coated white colorant to the surface of teeth are provided. The peptide-based reagent may comprise at least one tooth-binding peptide coupled to, either directly or through one or more spacers, a polymer-binding peptide capable of binding to the polymeric coating on a polymer-coated white colorant. Methods of delivering a polymer-coated white colorant to a tooth surface are also provided as well as oral care compositions comprising the present peptide-based reagents. The peptide-based reagents may be used in conjunction with at least one polymer-coated white colorant to improve the cosmetic appearance of teeth.

## Background
This application claims the benefit of U.S. Provisional Patent Application U.S. Provisional Patent Application No. 61/164,476, filed Mar. 30, 2009.


FIELD OF THE INVENTION
The invention relates to the use of peptide-based reagents to improve the cosmetic appearance of teeth. More specifically, peptide-based reagents that couple polymer-coated white colorants to teeth are provided. Oral care compositions comprising the present peptide-based reagents are also provided.
BACKGROUND OF THE INVENTION
The cosmetic appearance of teeth is of great importance to many individuals. These individuals typically desire a “bright” smile and white teeth. Unfortunately, the surface color of teeth generally dulls and discolors over time due to the absorbent nature of dental material.
Tooth coloration is influenced by a combination of intrinsic, such as age and genetics, and extrinsic factors, such as staining caused by various foods, beverages, medications, and tobacco use. Even with regular brushing and flossing, exposure to staining and discoloring substances over many years can cause noticeable discoloration. As such, there is a need for products and processes to quickly and safely whiten teeth.
One solution to the problem of tooth discoloration is the application of veneer facings made of porcelain, composites or ceramic. However, the application of veneer facings is expensive and requires the assistance of trained dental professional.
Bleaching agents may also be used to whiten teeth. The application of the bleaching agent may also require the assistance of a dental professional (i.e., application of concentrated oxidizing agents) and/or multiple applications and may not achieve the desired degree of whitening. Over-the-counter bleaching products typically use lower concentrations of the bleaching agent(s) and often require repeated application of the product. However, the use of bleaching agents has been associated with several undesirable side effects including chemical burns, irritation to the gums, and an increase in tooth sensitivity.
White colorants may also be used to whiten teeth. The non-toxic colorants are typically white pigments or a combination of white pigments with other non-white pigments to achieve a more “natural” white appearance. However, the use of pigments to improve the cosmetic appearance of teeth generally lacks the required durability to achieve the desired cosmetic effect.
There have been various attempts to enhance the binding durability of tooth whitening agents. U.S. Patent Application Publication No. 2005-0069501 to Ibrahim et al. describes the use of a siloxane adhesive and a whitening particulate (hydroxyapatite powder) as a tooth whitening composition. PCT publication WO2006/068011 by Shimako et al. discloses a tooth whitening composition comprising (A) one or more pigments, (B) pullulan, and (C) one or more members selected from lysozyme, cationized cellulose and poly lysine, wherein the components (B) and (C) are used to attach the metal oxide powder on the tooth surface. Neither reference discloses a tooth whitening system comprising a peptide-based reagent suitable for enhancing the durability of a polymer-coated white colorant.
Short peptides having strong affinity for various body surfaces have been identified using a biopanning technique, such as phage display (U.S. Pat. Nos. 7,220,405; 7,309,482; and 7,285,264; U.S. Patent Application Publication Nos. 2005-0226839; 2007-0196305; 2006-0199206; 2007-0065387; 2008-0107614; 2007-0110686; and 2006-0073111; and published PCT applications WO2008/054746; WO2004/048399, and WO2008/073368). However, peptide-based reagents comprising at least one portion having affinity for a tooth surface and at least one portion having affinity for a polymer-coated whitening agent are not described.
U.S. Patent Application Publication No. 2007/0065387 to Beck et al. describes the use of peptide-based reagent comprising at least one portion having strong affinity for hair or skin and at least one portion having affinity for a polymer-coated colorant. Oral care compositions comprising a peptide-based reagent and at least one polymer-coated white colorant are not provided. No tooth-binding peptides or cellulose acetate-binding peptides are described.
The problem to be solved is to provide compositions and methods for delivery of a polymer-coated white colorant to a tooth surface for whitening teeth.
SUMMARY OF THE INVENTION
Peptide-based reagents and methods of using the present reagents to whiten teeth are provided. The peptide-based reagents may be used in conjunction with at least one polymer-coated white colorant to whiten teeth.
Oral care compositions comprising at least one of the present peptide-based reagents are also provided. In a preferred embodiment, the oral care compositions further comprise at least one polymer-coated white colorant, such as a polymer-coated white pigment.
In one embodiment, a peptide-based reagent is provided selected from the group consisting of:

    
    
        a) a peptide-based reagent having the general structure:

[(TBP)m-(PBP)n]x; and

        b) a peptide-based reagent having the general structure:

[[(TBP)y-Sq]m-[(PBP)z-Sr]n]p;

        wherein
        
            i) TBP is a tooth-binding peptide;
            ii) PBP is a polymer-binding peptide;
            iii) S is a spacer;
            iv) m, n, x, y and z independently range from 1 to about 10;
            v) p is from 1 to 5; and
            vi) q an r are each independently 0 or 1, provided that both q and r may not be 0.
        
        
    
    


In one aspect, the present peptide-based reagents comprise polymer-binding peptides ranging from 7 amino acids to 50 amino acids in length and the tooth-binding peptides ranging from 7 to 50 amino acids in length. In a preferred embodiment, the tooth-binding peptide is from about 7 to about 25 amino acids in length and has a binding affinity for a tooth surface, measured as an MB50 value, equal to or less than 10−5 M.
In another aspect, the present peptide peptide-based reagents comprise at least one tooth-binding peptide having an amino acid sequence selected from the group consisting of SEQ ID NOs: 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, and 108.
In another aspect, the present peptide-based reagents comprise at least one tooth-binding peptide having an amino acid sequence selected from the group consisting of SEQ ID NOs: 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, and 191.
In another aspect, the present peptide-based reagent comprises a polymer-binding peptide the binds to a polymer selected from the group consisting of cellulose acetate, polyacrylates, polymethacrylates, polymethylmethacrylates, polycarbonates, polystyrene, polypropylene, polyethylene terephthalate, polyurethanes, polypeptides, lignin, polysaccharides, modified polysaccharides, polyamides, polyimides, polyaramides, polyethers, silicones, methicones, dimethicones and copolymers comprising at least one monomer from methacylates, acrylates or styrene.
In a preferred aspect, the polymer-binding peptide comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, and 118.
In another aspect, the polymer-coated white colorant may be coupled directly to at least one of the above tooth-binding peptides. As such, a peptide-based reagent having the following structure is also provided:

    
    
        a) (TBP)k-PCWC, wherein
        
            i) TBP is a tooth-binding peptide;
            ii) PCWC is a polymer-coated white colorant; and
            iii) k ranges from 1 to about 10,000; and
        
        
        b) [(TBP)v-L]k-PCWC, wherein
        
            i) TBP is a tooth-binding peptide;
            ii) PCWC is a polymer-coated white colorant;
            iii) L is a spacer;
            iv) v ranges from 1 to about 50; and
            v) k ranges from 1 to about 10,000.
        
        
    
    


In another embodiment, a method for whitening teeth is provided comprising:

    
    
        a) providing at least one polymer-coated white colorant;
        b) providing a composition comprising at least one of the present peptide-based reagents; and
        c) contacting a tooth surface with the at least one polymer-coated white colorant of (a) and the composition of (b) whereby said tooth surface is whitened.
    
    


In another embodiment, a method of delivering a polymer-coated white colorant to a tooth surface is provided comprising:

    
    
        a) providing at least one polymer-coated white colorant;
        b) providing a composition comprising at least one of the present peptide-based reagents; and
        c) applying said at least one polymer-coated pigment of (a) with the composition of (b) to a tooth surface for a period of time sufficient for the peptide-based tooth whitening reagent in composition of (b) to bind to the polymer-coated white colorant and the tooth surface.
    
    


In a further embodiment, the above methods may further comprise a step (d): applying a composition comprising a polymeric sealant; wherein the polymeric sealant is selected from the group consisting of poly(allylamine), acrylates, acrylate copolymers, polyurethanes, carbomers, methicones, amodimethicones, polyethylenene glycol, beeswax, siloxanes, copolymers of methyl vinyl ether and maleic anhydride, linear and cross linked polyvinylpyrrolidone homopolymers, and combinations thereof.
The present peptide-based reagents may be used in oral care compositions. As such, an oral care composition is also provided comprising at least one of the present peptide based-reagents and at least one polymer-coated white colorant. In a preferred aspect, the oral care composition further comprises at least one of the present peptide-based reagents in an orally-acceptable carrier medium.
In another aspect, a composition comprising at least one cellulose acetate coated pigment is provided. In a preferred aspect, an oral care composition comprising at least one cellulose acetate-coated pigment in an orally-acceptable carrier medium is also provided.
In another aspect, said cellulose acetate-coated pigment may comprise a core pigment particle coated with a first layer of silicon dioxide followed by a second layer applied over the said first layer of silicon dioxide wherein the second layer is cellulose acetate.
In another embodiment, the present peptide-based reagents comprise at least one cellulose acetate-binding peptide having an amino acid sequence selected from the group consisting of SEQ ID NOs: 60, 61, 62, 63, 64, 65, 66, 67, and 118.


BRIEF DESCRIPTION OF THE BIOLOGICAL SEQUENCES
The invention can be more fully understood from the following detailed description and the accompanying sequence descriptions, which form a part of this application.
The following sequences conform with 37 C.F.R. 1.821-1.825 (“Requirements for Patent Applications Containing Nucleotide Sequences and/or Amino Acid Sequence Disclosures—the Sequence Rules”) and consistent with World Intellectual Property Organization (WIPO) Standard ST.25 (1998) and the sequence listing requirements of the EPO and PCT (Rules 5.2 and 49.5(a-bis), and Section 208 and Annex C of the Administrative Instructions). The symbols and format used for nucleotide and amino acid sequence data comply with the rules set forth in 37 C.F.R. §1.822.
SEQ ID NOS: 1-67 and 118 are the amino acid sequence of polymer-binding peptides. SEQ ID NOs: 1-27 are sequences of peptides that bind to polymethylmethacrylate, SEQ ID NOs: 28-34 are sequences of peptides bind to polypropylene, SEQ ID NOs 35-43 are sequences of peptides that bind to polytetrafluoroethylene, SEQ ID NOs: 44-49 are sequences of peptides that bind to nylon, SEQ ID NOs: 50-56 are sequences of peptides that bind to polyethylene, SEQ ID NOs: 57-59 are sequences of peptides that bind to polystyrene, SEQ ID NOs: 60-67, and 118 are sequences of peptides that bind to cellulose acetate.
SEQ ID NO: 68 is the nucleic acid sequence of an oligonucleotide primer used to sequence phage DNA.
SEQ ID NOs: 69-108 and 119-149 are amino acid sequences of tooth-binding peptides. SEQ ID NOs: 69-88, 109-149, and 152-191 are sequences of peptides that bind to tooth pellicle. SEQ ID NOs: 69-108 are sequences of peptides that bind to tooth enamel.
SEQ ID NO: 109 is the amino acid sequence of the Caspase 3 cleavage site.
SEQ ID NOs: 110-115 are the amino acid sequences of peptide spacers.
SEQ ID NO: 116 is the amino acid sequence of a peptide-based reagent (DE020).
SEQ ID NO: 117 is the nucleic acid sequence of expression vector pKSI(C4)E-HC77643.
SEQ ID NO: 150 is the amino acid sequence of a peptide used as a control in Example 13.
SEQ ID NO: 151 is the amino acid sequence of an N-terminal tag described in Example 14.
DETAILED DESCRIPTION
Provided are peptide-based reagents, oral care compositions comprising one or more of the present reagents, and methods of their use. The peptide-based reagent may comprise a first portion comprising one or more tooth-binding peptides and a second portion comprising one or more polymer-binding peptides. The first and second portions may be optionally separated by one or more spacers. The individual tooth-binding and/or polymer-binding peptides may optionally be separated by one or more spacers.
In another embodiment, peptide-based whitening reagents comprising a polymer-coated white colorant coupled directly, or through an optional spacer, to a tooth-binding peptide are also provided.
The white colorant is coated with a polymer such that peptides having an affinity for the polymer, identified by combinatorial methods as described below, will bind to the polymer coating. The polymer coating may be formed from many different organic and biological polymers including, but not limited to cellulose acetate, polyacrylates, polymethacrylates, polymethylmethacrylates, polycarbonates, polystyrene, polypropylene, polyethylene terephthalate, polyurethanes, polypeptides, lignin, polysaccharides, modified polysaccharides, polyamides, polyimides, polyaramides, polyethers, silicones, methicones, dimethicones and copolymers (e.g., block and graft copolymers) comprising at least one monomer from methacylates, acrylates or styrene.
The following definitions are used herein and should be referred to for interpretation of the claims and the specification.
As used herein, the articles “a”, “an”, and “the” preceding an element or component of the invention are intended to be nonrestrictive regarding the number of instances (i.e., occurrences) of the element or component. Therefore “a”, “an” and “the” should be read to include one or at least one, and the singular word form of the element or component also includes the plural unless the number is obviously meant to be singular.
As used herein, the term “comprising” means the presence of the stated features, integers, steps, or components as referred to in the claims, but that it does not preclude the presence or addition of one or more other features, integers, steps, components or groups thereof. The term “comprising” is intended to include embodiments encompassed by the terms “consisting essentially of” and “consisting of”. Similarly, the term “consisting essentially of” is intended to include embodiments encompassed by the term “consisting of”.
As used herein, the term “about” refers to modifying the quantity of an ingredient or reactant of the invention or employed refers to variation in the numerical quantity that can occur, for example, through typical measuring and liquid handling procedures used for making concentrates or use solutions in the real world; through inadvertent error in these procedures; through differences in the manufacture, source, or purity of the ingredients employed to make the compositions or carry out the methods; and the like. The term “about” also encompasses amounts that differ due to different equilibrium conditions for a composition resulting from a particular initial mixture. Whether or not modified by the term “about”, the claims include equivalents to the quantities.
Where present, all ranges are inclusive and combinable. For example, when a range of “1 to 5” is recited, the recited range should be construed as including ranges “1 to 4”, “1 to 3”, “1-2”, “1-2 & 4-5”, “1-3 & 5”, and the like.
As used herein, the term “invention” or “present invention” is a non-limiting term and is not intended to refer to any single embodiment of the particular invention but encompasses all possible embodiments as described in the specification and the claims.
As used herein, the term “peptide” refers to two or more amino acids joined to each other by peptide bonds or modified peptide bonds.
As used herein, “TBP” means tooth-binding peptide. A tooth-binding peptide is a peptide that binds with high affinity to a tooth surface.
The term “tooth surface” will refer to a surface comprised of tooth enamel (typically exposed after professional cleaning or polishing) or tooth pellicle (a surface comprising salivary glycoproteins). Hydroxyapatite can be coated with salivary glycoproteins to mimic a natural tooth pellicle surface (tooth enamel is predominantly comprised of hydroxyapatite).
As used herein, the terms “pellicle” and “tooth pellicle” will refer to the thin film (typically ranging from about 20 nm to about 200 μm thick) derived from salivary glycoproteins which forms over the surface of the tooth crown. Daily tooth brushing tends to only remove a portion of the pellicle surface while abrasive tooth cleaning and/or polishing (typically by a dental professional) will expose more of the tooth enamel surface.
As used herein, the terms “enamel” and “tooth enamel” will refer to the highly mineralized tissue which forms the outer layer of the tooth. The enamel layer is composed primarily of crystalline calcium phosphate (i.e., hydroxyapatite) along with water and some organic material. In one embodiment, the tooth surface is selected from the group consisting of tooth enamel and tooth pellicle.
As used herein, “TSBP” means target surface-binding peptide(s). The target surface-binding peptides described herein may refer to tooth-binding peptides and polymer-binding peptides. The target-surface-binding peptides typically range in size from 7 to about 50 amino acids in length. Individual target surface-binding peptides may be referred to herein as a binding “fingers”. Linking together multiple “fingers” forms a binding domain or binding “hand” for the respective target surface. In one embodiment, each “finger” is a combinatorially-generated peptide isolated using biopanning, such as phage display or mRNA display
As used herein, the term “tooth-binding peptide” will refer to a peptide that binds to tooth enamel or tooth pellicle. In one embodiment, the tooth-binding peptides are from about 7 amino acids to about 50 amino acids in length, more preferably, from about 7 amino acids to about 25 amino acids in length, most preferably from about 7 to about 20 amino acids in length. In a preferred embodiment, the tooth-binding peptides are combinatorially-generated peptides. The tooth-binding peptides (“fingers”) may be linked together to form tooth-binding domains (“hands”).
As used herein, “PBP” means polymer-binding peptide. A polymer-binding peptide is a peptide that binds with high affinity to a specified polymer. The polymer is used to coat the white colorant. As such, the polymer coating provides a binding interface between the polymer-binding peptide and the white colorant. The polymer-binding peptide is selected based on the polymer used to coat the white colorant. A non-limiting list of polymer-binding peptides is provided in Table A. In one embodiment, the polymer-binding peptides are from about 7 amino acids to about 50 amino acids in length, more preferably, from about 7 amino acids to about 25 amino acids in length, most preferably from about 7 to about 20 amino acids in length. In one embodiment, the polymer-binding peptide is a combinatorially-generated peptide. The polymer-binding peptides (“fingers”) may be linked together to form polymer-binding domains (“hands”).
As used herein, “S” and “L” mean spacer. The spacer may be an organic spacer and/or a peptide spacer. In one embodiment, the spacer is selected from the group consisting of ethanolamine, ethylene glycol, polyethylene with a chain length of 6 carbon atoms, polyethylene glycol with 3 to 6 repeating units, phenoxyethanol, propanolamide, butylene glycol, butyleneglycolamide, propyl phenyl, ethyl alkyl chain, propyl alkyl chain, hexyl alkyl chain, steryl alkyl chains, cetyl alkyl chains, and palmitoyl alkyl chains.
In another embodiment, the spacer is a peptide spacer. In one embodiment, the spacer or linker is a peptide linker. In one aspect, the peptide linker comprises 1 to 50 amino acids in length, preferably 1 to 25 amino acids, and most preferably 1 to 10 amino acids in length. In one aspect, the peptide spacer comprises amino acids selected from the group consisting of proline, lysine, glycine, alanine, serine, threonine, and combinations thereof. In a further aspect, the peptide spacer may have an amino acid sequence selected from the group consisting of SEQ ID NOs: 109-115. Depending upon the location of the peptide spacer in the peptide-based reagent, the peptide spacer may also be referred to as a “peptide linker” or a “peptide bridge”. In one embodiment, the “spacer” may be a peptide linker. In another embodiment, the spacer may be a peptide bridge used to separate two or more binding domains.
As used herein, a “polymer” is a natural or synthetic compound of usually high molecular weight consisting of repeated linked units. In one embodiment, the natural polymer used to coat the white colorant is not comprised of polynucleotides, polypeptides or combinations thereof.
As used herein, the terms “polymer-coated white colorant” and “white colorant” are abbreviated as “PCWC” and mean a polymer-coated colorant particle capable of providing a whitening effect when coupled to a tooth surface.
As used herein, the terms “peptide-based reagent” and “peptide-based whitening reagent” will be used to refer to a single chain peptide comprising at least one portion having affinity for a tooth surface and at least one portion having affinity for a polymer comprising an effective amount of the corresponding polymer. The peptide-based reagent may range in size from about 14 to about 600 amino acids in length and is not comprised of an immunoglobulin fold and does not require scaffold-assisted binding (i.e., the present peptide-based reagent is not comprised of a naturally-occurring scaffold protein used in scaffold-assisted binding; See Binz, H. et al. (2005) Nature Biotechnology 23, 1257-1268 for a review of various scaffold proteins used in scaffold-assisted binding). In a preferred aspect, the peptide-based reagent is about 14 to about 200 amino acids in length. The peptide-based reagent is typically comprised of a first domain having affinity for a tooth surface and a second domain having affinity for a surface comprising the respective polymer. The peptide-based reagent may further comprise a “peptide bridge” separating the first domain from the second domain. The first and/or second domains may be comprised of a single peptide “finger” or may be comprised of a plurality of target surface-binding peptides, optionally separated with one or more peptide linkers.
As used herein, the term “whitening” refers to a change in visual appearance of a tooth, preferably such that the tooth has a brighter shade. An increase in white can be determined visually or may be measured by colorimetry as described below.
As used herein, the term “pigment” means an insoluble colorant. A wide variety of organic and inorganic pigments alone or in combination may be used in the present invention.
As used herein, the term “pigment lake” or “lake” refers to a pigment manufactured by precipitating a dye with an inert binder, usually a metallic salt.
The terms “coupling” and “coupled” as used herein refer to any chemical association and includes both covalent and non-covalent interactions. In one embodiment, non-covalent interactions are used to couple the polymer-coated white colorant to the tooth surface using the present peptide-based reagents.
The term “stringency” as it is applied to the selection of the target surface-binding peptides (e.g., tooth-binding peptides, polymer-binding peptides), refers to the concentration of the eluting agent (usually detergent) used to elute peptides from the target surface. Higher concentrations of the eluting agent provide more stringent conditions. Many of the present tooth-binding and polymer-binding peptides were selected under highly stringent conditions (i.e. those resistant to washing conditions that included 0.5 wt % TWEEN®).
The term “peptide-tooth complex” means structure comprising a peptide bound to a tooth surface via a binding site on the peptide.
As used herein, the term “peptide-polymer complex” means structure comprising a peptide bound to the surface of a specified polymer via a binding site on the peptide. The polymer may be coated on a solid support, such as a metal oxide pigment.
As used herein, the term “effective amount of polymer” or “effective amount of polymeric coating” refers to an amount of polymer that provides a surface capable of binding to one or more of the present polymer-binding peptides, preferably with strong affinity. One of skill in the art can determine an effective amount by measuring an increase in binding of the polymer-coated white colorant to a tooth surface when used in combination with one or more of the present peptide-based reagents.
The term “MB50” refers to the concentration of the binding peptide that gives a signal that is 50% of the maximum signal obtained in an ELISA-based binding assay (see U.S. Published Patent Application No. 2005-0226839; hereby incorporated by reference). The MB50 provides an indication of the strength of the binding interaction or affinity of the components of the complex. The lower the value of MB50, the stronger the interaction of the peptide with its corresponding substrate.
As used herein, the terms “binding affinity” and “affinity” refer to the strength of the interaction of a binding peptide (such as target surface-binding peptides, target surface-binding domains, and peptide reagents) with its respective substrate. The binding affinity may be reported in terms of the MB50 value as determined in an ELISA-based binding assay or as a KD (equilibrium dissociation constant) value, which may be deduced using surface plasmon resonance (SPR).
As used herein, the terms “strong affinity” and “high affinity” refer to a binding affinity, as measured as an MB50 value of KD value, of 10−4 M or less, preferably less than 10−5 M, more preferably less than 10−6 M, more preferably less than 10−7 M, even more preferably less than 10−8 M, and most preferably less than 10−9 M.
The term “amino acid” refers to the basic chemical structural unit of a protein or polypeptide. The following abbreviations are used herein to identify specific amino acids:













Three-Letter
One-Letter


Amino Acid
Abbreviation
Abbreviation







Alanine
Ala
A


Arginine
Arg
R


Asparagine
Asn
N


Aspartic acid
Asp
D


Cysteine
Cys
C


Glutamine
Gln
Q


Glutamic acid
Glu
E


Glycine
Gly
G


Histidine
His
H


Isoleucine
Ile
I


Leucine
Leu
L


Lysine
Lys
K


Methionine
Met
M


Phenylalanine
Phe
F


Proline
Pro
P


Serine
Ser
S


Threonine
Thr
T


Tryptophan
Trp
W


Tyrosine
Tyr
Y


Valine
Val
V


Any naturally-occurring amino acid
Xaa
X


(or as defined herein)









“Gene” or genetic construct” refers to a nucleic acid fragment that expresses a specific protein, optionally including regulatory sequences preceding (5′ non-coding sequences) and following (3′ non-coding sequences) the coding sequence. “Native gene” refers to a gene as found in nature with its own regulatory sequences “Chimeric gene” refers to any gene that is not a native gene, comprising regulatory and coding sequences that are not found together in nature. Accordingly, a chimeric gene may comprise regulatory sequences and coding sequences that are derived from different sources, or regulatory sequences and coding sequences derived from the same source, but arranged in a manner different than that found in nature. A “foreign” gene refers to a gene not normally found in the host organism, but that is introduced into the host organism by gene transfer. Foreign genes can comprise native genes inserted into a non-native organism, or chimeric genes.
“Promoter” refers to a DNA sequence capable of controlling the expression of a coding sequence or functional RNA. In general, a coding sequence is located 3′ to a promoter sequence. Promoters may be derived in their entirety from a native gene, or be composed of different elements derived from different promoters found in nature, or even comprise synthetic DNA segments. It is understood by those skilled in the art that different promoters may direct the expression of a gene in different tissues or cell types, or at different stages of development, or in response to different environmental or physiological conditions. Promoters which cause a gene to be expressed in most cell types at most times are commonly referred to as “constitutive promoters”. It is further recognized that since in most cases the exact boundaries of regulatory sequences have not been completely defined, DNA fragments of different lengths may have identical promoter activity.
The terms “plasmid”, “vector” and “cassette” refer to an extra chromosomal element often carrying genes which are not part of the central metabolism of the cell, and usually in the form of circular double-stranded DNA molecules. Such elements may be autonomously replicating sequences, genome integrating sequences, phage or nucleotide sequences, linear or circular, of a single- or double-stranded DNA or RNA, derived from any source, in which a number of nucleotide sequences have been joined or recombined into a unique construction which is capable of introducing a promoter fragment and DNA sequence for a selected gene product along with appropriate 3′ untranslated sequence into a cell. “Transformation cassette” refers to a specific vector containing a foreign gene and having elements in addition to the foreign gene that facilitate transformation of a particular host cell. “Expression cassette” refers to a specific vector containing a foreign gene and having elements in addition to the foreign gene that allow for enhanced expression of that gene in a foreign host.
The term “phage” or “bacteriophage” refers to a virus that infects bacteria. Altered forms may be used for the purpose of the present invention. The preferred bacteriophage is derived from the “wild” phage, called M13. The M13 system can grow inside a bacterium, so that it does not destroy the cell it infects but causes it to make new phages continuously. It is a single-stranded DNA phage.
The term “phage display” refers to the display of functional foreign peptides or small proteins on the surface of bacteriophage or phagemid particles. Genetically engineered phage may be used to present peptides as segments of their native surface proteins. Peptide libraries may be produced by populations of phage with different gene sequences.
As used herein, the term “genetic construct” refers to a series of contiguous nucleic acids useful for modulating the genotype or phenotype of an organism. Non-limiting examples of genetic constructs include but are not limited to a nucleic acid molecule, an open reading frame, a gene, a plasmid, and the like.
As used herein, the term “peptide-based” refers to an interfacial material comprised of a compound pertaining to or having the nature or the composition of the peptide class. Interfacial refers to the quality of the peptide-based material described herein as connecting one material to another.
Standard recombinant DNA and molecular cloning techniques used herein are well known in the art and are described by Sambrook, J. and Russell, D., Molecular Cloning: A Laboratory Manual, Third Edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (2001); and by Silhavy, T. J., Bennan, M. L. and Enquist, L. W., Experiments with Gene Fusions, Cold Spring Harbor Laboratory Cold Press Spring Harbor, N.Y. (1984); and by Ausubel, F. M. et. al., Short Protocols in Molecular Biology, 5th Ed. Current Protocols and John Wiley and Sons, Inc., N.Y., 2002.
Target Surface-Binding Peptides
The present peptide-based reagents are comprised of at least one tooth-binding peptide (TBP). In another embodiment, the peptide-based reagents are comprised of at least one tooth-binding peptide and at least one polymer-binding peptide (PBP). The peptide-based reagents optionally comprise one or more spacers.
Tooth-binding peptides as defined herein are peptide sequences that bind with high affinity to mammalian teeth, preferably human teeth. The tooth surface may be tooth enamel or tooth pellicle, the acquired glycoprotein coating on teeth.
Examples of tooth-binding peptides having been disclosed in co-pending and co-owned U.S. Patent Application Publication No. 2008-0280810 and U.S. Provisional Patent Application No. 61/164,476 and are provided in Table A and Tables 14, 16, and 18. In one embodiment, the tooth-binding peptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, and 191. In a preferred embodiment, the tooth-binding peptide comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, and 191.
Polymer-binding peptides have been previously reported. Peptides having a binding affinity to polymer and plastic surfaces have been identified using phage display. For example, Adey et al., (Gene 156:27-31 (1995)) describe peptides that bind to polystyrene and polyvinyl chloride surfaces. Cunningham et al. describes in several co-pending and co-owned U.S. patent applications peptides that bind to polymethylmethacrylate (“PMMA”; U.S. App. Pub. No. 2007/0265431), polypropylene (U.S. App. Pub. No. 2007/0264720), nylon (U.S. App. Pub. No. 2007/0141629), polytetrafluoroethylene (“PTFE”; U.S. App. No. 11/607,734), polyethylene (“PE”; U.S. App. Pub. No. 2007/0141628), and polystyrene (“PS”; U.S. App. Pub. No. 2007/0261775). Additionally, peptides that bind to polyurethane (Murray et al., U.S. App. Pub. No. 2002/0098524), polyethylene terephthalate (O'Brien et al., co-pending and commonly owned U.S. App. Pub. No. 2005/0054752), and polystyrene, polyurethane, polycarbonate, and nylon (Grinstaff et al., U.S. App. Pub. No. 2003/0185870) have also been reported.
In another embodiment, the polymer-binding peptide comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, and 118. In one aspect, the white colorant is coated with cellulose acetate. As such, and in another embodiment, the polymer-binding peptide is a cellulose acetate-binding peptide. In a further embodiment, the cellulose acetate-binding peptide comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 60, 61, 62, 63, 64, 65, 66, 67, and 118. In a preferred embodiment, the cellulose acetate-binding peptide comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 61, 62, 63, and 118. In a further embodiment, the cellulose acetate-binding peptide comprises the amino acid sequence SEQ ID NO: 118.
Peptides having an affinity for a selected target surface (i.e., “target surface-binding peptides”) may be obtained using combinatorial methods or may be empirically generated. The tooth surface-binding peptides may have a binding affinity for a tooth surface, as measured by MB50 values, of less than or equal to about 10−3M, preferably less than or equal to about 10−4 M, more preferably less than or equal to about 10−5 M, more preferably less than or equal to about 10−6 M, even more preferably less than or equal to about 10−7 M, and even more preferably less than or equal to about 10−8 M. In one embodiment, the term “high affinity” or “strong affinity” will be used to describe tooth surface-binding peptides or polymer-binding peptides having a binding affinity, as measured by MB50 value, of less than or equal to about 10−5M, preferably less than or equal to about 10−6 M, more preferably less than or equal to about 10−7 M, and even more preferably less than or equal to about 10−8.
The generation of random libraries of peptides is well known and may be accomplished by a variety of techniques including, but not limited to bacterial display (Kemp, D. J.; Proc. Natl. Acad. Sci. USA 78(7): 4520-4524 (1981); yeast display (Chien et al., Proc Natl Acad Sci USA 88(21): 9578-82 (1991)), combinatorial solid phase peptide synthesis (U.S. Pat. No. 5,449,754; U.S. Pat. No. 5,480,971; U.S. Pat. No. 5,585,275 and U.S. Pat. No. 5,639,603), phage display technology (U.S. Pat. No. 5,223,409; U.S. Pat. No. 5,403,484; U.S. Pat. No. 5,571,698; and U.S. Pat. No. 5,837,500), ribosome display (U.S. Pat. No. 5,643,768; U.S. Pat. No. 5,658,754; and U.S. Pat. No. 7,074,557), and mRNA display technology (PROFUSIONTM; U.S. Pat. No. 6,258,558; U.S. Pat. No. 6,518,018; U.S. Pat. No. 6,281,344; U.S. Pat. No. 6,214,553; U.S. Pat. No. 6,261,804; U.S. Pat. No. 6,207,446; U.S. Pat. No. 6,846,655; U.S. Pat. No. 6,312,927; U.S. Pat. No. 6,602,685; U.S. Pat. No. 6,416,950; U.S. Pat. No. 6,429,300; U.S. Pat. No. 7,078,197; and U.S. Pat. No. 6,436,665). Techniques to generate such biological peptide libraries are described in Dani, M., J. of Receptor & Signal Transduction Res., 21(4):447-468 (2001). Additionally, phage display libraries are available commercially from companies such as New England BioLabs (Beverly, Mass.).
A preferred method to randomly generate peptides is by phage display. Phage display is an in vitro selection technique in which a peptide or protein is genetically fused to a coat protein of a bacteriophage, resulting in display of the fused peptide on the exterior of the phage virion, while the DNA encoding the fusion resides within the virion. This physical linkage between the displayed peptide and the DNA encoding it allows screening of vast numbers of variants of peptides, each linked to a corresponding DNA sequence, by a simple in vitro selection procedure called “biopanning”. In its simplest form, biopanning is carried out by incubating the pool of phage-displayed variants with a target of interest that has been immobilized on a plate or bead, washing away unbound phage, and eluting specifically bound phage by disrupting the binding interactions between the phage and the target. The eluted phage is then amplified in vivo and the process is repeated, resulting in a stepwise enrichment of the phage pool in favor of the tightest binding sequences. After 3 or more rounds of selection/amplification, individual clones are characterized by DNA sequencing.
More specifically, after a suitable library of peptides has been generated or purchased, the library is then contacted with an appropriate amount of the test substrate. The library of peptides is dissolved in a suitable solution for contacting the sample. The sample (i.e. target material have a target surface) is typically suspended in solution or may be immobilized on a plate or bead. A preferred solution is a buffered aqueous saline solution containing a surfactant. A suitable solution is Tris-buffered saline (TBS) with 0.5% TWEEN® 20. The solution may additionally be agitated by any means in order to increase the mass transfer rate of the peptides to the target sample/surface, thereby shortening the time required to attain maximum binding.
Upon contact, a number of the randomly generated peptides will bind to the target surface to form a peptide-target surface complex, for example, a peptide-siliceous particle complex. Unbound peptide may be removed by washing. After all unbound material is removed, peptides having varying degrees of binding affinities for the test surface may be fractionated by selected washings in buffers having varying stringencies. Increasing the stringency of the buffer used increases the required strength of the bond between the peptide and target surface in the peptide-target surface complex.
A number of substances may be used to vary the stringency of the washing solution in the peptide selection process including, but not limited to acids (pH 1.5-3.0), bases (pH 10-12.5), salts of high concentrations such as MgCl2 (3-5 M) and LiCl (5-10 M), ethylene glycol (25-50%), dioxane (5-20%), thiocyanate (1-5 M), guanidine (2-5 M), urea (2-8 M), and surfactants of various concentrations such as SDS (sodium dodecyl sulfate), DOC (sodium deoxycholate), Nonidet P-40, Triton X-100, shampoo (useful when selecting peptides for use in personal care compositions, such as a commercial shampoo formulation), TWEEN® 20, wherein TWEEN® 20 is more typical. These substances may be prepared in buffer solutions including, but not limited to, Tris-HCl, Tris-buffered saline, Tris-borate, Tris-acetic acid, triethylamine, phosphate buffer, and glycine-HCl, wherein Tris-buffered saline solution is preferred.
It will be appreciated that peptides having increasing binding affinities for target surface substrates may be eluted by repeating the selection process using buffers with increasing stringencies. The eluted peptides can be identified and sequenced by any means known in the art.
As many of the peptide-based reagents may be used in personal care products comprising significant amounts of surfactants/detergents (for example, sodium lauryl sulfate (SLS) is commonly used in oral care products such as toothpaste), the stringency of the washing steps may be increased to select only those peptides having the highest binding affinity. In one embodiment, the washing conditions includes a surfactant/detergent, such as a commercially available shampoo. In another embodiment, the washing conditions will include at least 1 wt % surfactant/detergent (e.g. shampoo), preferably at least 5 wt %, even more preferably at least 10 wt %, even more preferably at least 20 wt %, and most preferably at least 30 wt % surfactant/detergent. In one embodiment, peptides that are resistant to washing conditions including a specified surfactant/detergent will be referred to herein as “surfactant/detergent resistant” (or “shampoo resistant” if shampoo formulation used).
Thus, the following method for generating the target surface-binding peptides may be used. A library of combinatorially generated phage-peptides is contacted with the target surface of interest (e.g., a tooth surface or a polymeric surface used to coat the white colorant), to form phage peptide-target surface complexes. The phage-peptide-target surface complex is separated from uncomplexed peptides and unbound substrate, and the bound phage-peptides from the phage-peptide-target surface complexes is eluted from the complex, preferably by acid treatment. Then, the eluted phage-peptides are identified and sequenced. To identify peptide sequences that bind to one substrate but not to another, for example peptides that bind to another surface (i.e., a “non-target” surface; for example, another inorganic material or a body surface such as hair, skin, nail, etc.), a subtractive panning step may be added. Specifically, the library of combinatorially generated phage-peptides is first contacted with the non-target to remove phage-peptides that bind to it. Then, the non-binding phage-peptides are contacted with the desired substrate and the above process is followed. Alternatively, the library of combinatorially generated phage-peptides may be contacted with the non-target and the desired substrate simultaneously. Then, the phage-peptide-target surface complexes are separated from the phage-peptide-non-target complexes and the method described above is followed for the desired phage-peptide-target surface complexes.
In one embodiment, a modified phage display screening method for isolating peptides with a higher affinity for the target surface is used. In the modified method, the phage-peptide-target surface complexes are formed as described above. Then, these complexes are treated with an elution buffer. Any of the elution buffers described above may be used. Preferably, the elution buffer is an acidic solution. Then, the remaining, elution-resistant phage-peptide-target surface complexes are used to directly infect a bacterial host cell, such as E. coli ER2738. The infected host cells are grown in an appropriate growth medium, such as LB (Luria-Bertani) medium, and this culture is spread onto agar, containing a suitable growth medium, such as LB medium with IPTG (isopropyl β-D-thiogalactopyranoside) and S-GALTM. After growth, the plaques are picked for DNA isolation and are sequenced to identify the peptide sequences with a high binding affinity for the target surface of interest.
In another embodiment, PCR may be used to identify the elution-resistant phage-peptides from the modified phage display screening method, described above, by directly carrying out PCR on the phage-peptide-target surface complexes using the appropriate primers, as described by Janssen et al. in U.S. App. Pub. No. 2003/0152976, which is incorporated herein by reference.
Examples of various target surface-binding peptides are provided in Table A.







TABLE A







Examples of Target Surface-Binding Peptides










Surface*
Amino Acid Sequence
SEQ ID NO:
Reference














PMMA
IPWWNIRAPLNA
1
US 2007-0265431



 


PMMA
TAVMNVVNNQLS
2
US 2007-0265431


 


PMMA
VPWWAPSKLSMQ
3
US 2007-0265431


 


PMMA
MVMAPHTPRARS
4
US 2007-0265431


 


PMMA
TYPNWAHLLSHY
5
US 2007-0265431


 


PMMA
TPWWRIT
6
US 2007-0265431


 


PMMA
DLTLPFH
7
US 2007-0265431


 


PMMA
GTSIPAM
8
US 2007-0265431


 


PMMA
HHKHVVA
9
US 2007-0265431


 


PMMA
HHHKHFM
10
US 2007-0265431


 


PMMA
HHHRHQG
11
US 2007-0265431


 


PMMA
HHWHAPR
12
US 2007-0265431


 


PMMA
APWHLSSQYSGT
13
US 2007-0065387


 


PMMA
GYCLRVDEPTVCSG
14
US 2007-0065387


 


PMMA
HIHPSDNFPHKNRTH
15
US 2007-0065387


 


PMMA
HTHHDTHKPWPTDDHRNSSV
16
US 2007-0065387


 


PMMA
PEDRPSRTNALHHNAHHHNA
17
US 2007-0065387


 


PMMA
TPHNHATTNHHAGKK
18
US 2007-0065387


 


PMMA
EMVKDSNQRNTRISS
19
US 2007-0065387


 


PMMA
HYSRYNPGPHPL
20
US 2007-0065387


 


PMMA
IDTFYMSTMSHS
21
US 2007-0065387


 


PMMA
PMKEATHPVPPHKHSETPTA
22
US 2007-0065387


 


PMMA
YQTSSPAKQSVG
23
US 2007-0065387


 


PMMA
HLPSYQITQTHAQYR
24
US 2007-0065387


 


PMMA
TTPKTTYHQSRAPVTAMSEV
25
US 2007-0065387


 


PMMA
DRIHHKSHHVTTNHF
26
US 2007-0065387


 


PMMA
WAPEKDYMQLMK
27
US 2007-0065387


 


PP
TSDIKSRSPHHR
28
US 2007-0264720


 


PP
HTQNMRMYEPWF
29
US 2007-0264720


 


PP
LPPGSLA
30
US 2007-0264720


 


PP
MPAVMSSAQVPR
31
US 2007-0264720


 


PP
NQSFLPLDFPFR
32
US 2007-0264720


 


PP
SILSTMSPHGAT
33
US 2007-0264720


 


PP
SMKYSHSTAPAL
34
US 2007-0264720


 


PTFE
ESSYSWSPARLS
35
US 11/607,734


 


PTFE
GPLKLLHAWWQP
36
US 11/607,734


 


PTFE
NALTRPV
37
US 11/607,734


 


PTFE
SAPSSKN
38
US 11/607,734


 


PTFE
SVSVGMKPSPRP
39
US 11/607,734


 


PTFE
SYYSLPPIFHIP
40
US 11/607,734


 


PTFE
TFTPYSITHALL
41
US 11/607,734


 


PTFE
TMGFTAPRFPHY
42
US 11/607,734


 


PTFE
TNPFPPPPSSPA
43
US 11/607,734


 


nylon
KTPPTRP
44
US 2007-0141629


 


nylon
VINPNLD
45
US 2007-0141629


 


nylon
KVWIVST
46
US 2007-0141629


 


nylon
AEPVAML
47
US 2007-0141629


 


nylon
AELVAML
48
US 2007-0141629


 


nylon
HSLRLDW
49
US 2007-0141629


 


PE
HNKSSPLTAALP
50
US 2007-0141628


 


PE
LPPWKHKTSGVA
51
US 2007-0141628


 


PE
LPWWLRDSYLLP
52
US 2007-0141628


 


PE
VPWWKHPPLPVP
53
US 2007-0141628


 


PE
HHKQWHNHPHHA
54
US 2007-0141628


 


PE
HIFSSWHQMWHR
55
US 2007-0141628


 


PE
WPAWKTHPILRM
56
US 2007-0141628


 


PS
TSTASPTMQSKIR
57
US 2007-0261775


 


PS
KRNHWQRMHLSA
58
US 2007-0261775


 


PS
SHATPPQGLGPQ
59
US 2007-0261775


 


CA
ATTPPSGKAAAHSAARQKGN
60
Disclosed herein


 


CA
DTIHPNKMKSPSSPL
61
Disclosed herein


 


CA
NGNNHTDIPNRSSYTGGSFA
62
Disclosed herein


 


CA
SDETGPQIPHRRPTW
63
Disclosed herein


 


CA
ATTPPSGKAAAHSAARQKGNK
64
Disclosed herein


 


CA
DTIHPNKMKSPSSPLK
65
Disclosed herein


 


CA
NGNNHTDIPNRSSYTGGSFAK
66
Disclosed herein


 


CA
SDETGPQIPHRRPTWK
67
Disclosed herein


 


CA
VRPNLHRKAKAKPDHKQSENR
118
US 60/972,307



KVPFYSHGSSG


 


Tooth
AHPESLGIKYALDGNSDPHA
69
US 11/877,692


(pellicle)


 


Tooth
ASVSNYPPIHHLATSNTTVN
70
US 11/877,692


(pellicle)


 


Tooth
DECMEPLNAAHCWR
71
US 11/877,692


(pellicle)


 


Tooth
DECMHGSDVEFCTS
72
US 11/877,692


(pellicle)


 


Tooth
DLCSMQMMNTGCHY
73
US 11/877,692


(pellicle)


 


Tooth
DLCSSPSTWGSCIR
74
US 11/877,692


(pellicle)


 


Tooth
DPNESNYENATTVSQPTRHL
75
US 11/877,692


(pellicle)


 


Tooth
EPTHPTMRAQMHQSLRSSSP
76
US 11/877,692


(pellicle)


 


Tooth
GNTDTTPPNAVMEPTVQHKW
77
US 11/877,692


(pellicle)


 


Tooth
NGPDMVQSVGKHKNS
78
US 11/877,692


(pellicle)


 


Tooth
NGPEVRQIPANFEKL
79
US 11/877,692


(pellicle)


 


Tooth
NNTSADNPPETDSKHHLSMS
80
US 11/877,692


(pellicle)


 


Tooth
NNTWPEGAGHTMPSTNIRQA
81
US 11/877,692


(pellicle)


 


Tooth
NPTATPHMKDPMHSNAHSSA
82
US 11/877,692


(pellicle)


 


Tooth
NPTDHIPANSTNSRVSKGNT
83
US 11/877,692


(pellicle)


 


Tooth
NPTDSTHMMHARNHE
84
US 11/877,692


(pellicle)


 


Tooth
QHCITERLHPPCTK
85
US 11/877,692


(pellicle)


 


Tooth
TPCAPASFNPHCSR
86
US 11/877,692


(pellicle)


 


Tooth
TPCATYPHFSGCRA
87
US 11/877,692


(pellicle)


 


Tooth
WCTDFCTRSTPTSTSRSTTS
88
US 11/877,692


(pellicle)


 


Tooth
APPLKTYMQERELTMSQNKD
89
US 11/877,692


(enamel)


 


Tooth
EPPTRTRVNNHTVTVQAQQH
90
US 11/877,692


(enamel)


 


Tooth
GYCLRGDEPAVCSG
91
US 11/877,692


(enamel)


 


Tooth
LSSKDFGVTNTDQRTYDYTT
92
US 11/877,692


(enamel)


 


Tooth
NFCETQLDLSVCTV
93
US 11/877,692


(enamel)


 


Tooth
NTCQPTKNATPCSA
94
US 11/877,692


(enamel)


 


Tooth
PSEPERRDRNIAANAGRFNT
95
US 11/877,692


(enamel)


 


Tooth
THNMSHFPPSGHPKRTAT
96
US 11/877,692


(enamel)


 


Tooth
TTCPTMGTYHVCWL
97
US 11/877,692


(enamel)


 


Tooth
YCADHTPDPANPNKICGYSH
98
US 11/877,692


(enamel)


 


Tooth
AANPHTEWDRDAFQLAMPPK
99
US 11/877,692


(enamel)


 


Tooth
DLHPMDPSNKRPDNPSDLHT
100
US 11/877,692


(enamel)


 


Tooth
ESCVSNALMNQCIY
101
US 11/877,692


(enamel)


 


Tooth
HNKADSWDPDLPPHAGMSLG
102
US 11/877,692


(enamel)


 


Tooth
LNDQRKPGPPTMPTHSPAVG
103
US 11/877,692


(enamel)


 


Tooth
NTCATSPNSYTCSN
104
US 11/877,692


(enamel)


 


Tooth
SDCTAGLVPPLCAT
105
US 11/877,692


(enamel)


 


Tooth
TIESSQHSRTHQQNYGSTKT
106
US 11/877,692


(enamel)


 


Tooth
VGTMKQHPTTTQPPRVSATN
107
US 11/877,692


(enamel)


 


Tooth
YSETPNDQKPNPHYKVSGTK
108
US 11/877,692


(enamel)





*PMMA means polymethylmethacrylate, PP means polypropylene, PTFE means polytetrafluoroethylene, PE means polyethylene, PS means polystyrene, CA means cellulose acetate.






Tooth Surfaces

The tooth surface is a natural surface on mammalian teeth that can serve as a substrate for a binding peptide. In one embodiment, the tooth surface is selected from tooth enamel and tooth pellicle.
Samples of tooth surfaces are available from a variety of sources. For example, extracted mammalian teeth, such as bovine and/or human teeth are commercially available. Extracted human teeth (with or without a pellicle coating) may also be obtained from dental offices. Additionally, hydroxyapatite, available in many forms, for example, from Berkeley Advanced Biomaterials, Inc. (San Leandro, Calif.), may be used (preferably coated with salivary glycoproteins to form an acquired pellicle surface) as a model for studying teeth-binding peptides (see co-owned and co-pending U.S. application Ser. No. 11/877,692).
White Colorants
The present invention is based on the use of a polymer-coated white colorant. Any number of orally-acceptable white colorants may be used. In one embodiment, the white colorant is a white pigment.
A wide variety of organic and inorganic pigments alone or in combination may be used in the present invention, so long as the colorant or combination of colorants result in the desired cosmetic effect (i.e. whiter teeth). Pigments for use in personal care products are well known in the art (see for example Green et al. (WO 0107009), CFTA International Color Handbook, 2nd ed., Micelle Press, England (1992) and Cosmetic Handbook, US Food and Drug Administration, FDA/IAS Booklet (1992)), and are available commercially from various sources (for example Bayer, Pittsburgh, Pa.; Ciba-Geigy, Tarrytown, N.Y.; ICI, Bridgewater, N.J.; Sandoz, Vienna, Austria; BASF, Mount Olive, N.J.; and Hoechst, Frankfurt, Germany).
The size (as defined the average equivalent diameter or “average diameter”) of the polymer-coated pigments may vary. In one embodiment the average diameter will range from about 10 nanometers (nm) to about 50 micrometers (μ), preferably about 10 nm to about 20 μm, more preferably 50 nm to about 20 μm, and most preferably about 100 nm to about 2 μm. The pigments can be partially or completely coated in the desired polymer and may be porous or non-porous. The polymer coating thickness on the white colorant may vary. Typically, the coating thickness ranges from about 2 nm to about 50 nm, preferably about 5 nm to about 20 nm.
In a preferred embodiment, the polymer-coated white colorant is a polymer-coated white pigment. However, to achieve a more natural appearance it may be necessary to also include one or more non-white pigments. As such, any of the following pigments or combinations thereof may also be used.
Exemplary pigments may include, but are not limited to, D&C Red No. 36, D&C Red No. 30, D&C Orange No. 17, Green 3 Lake, Ext. Yellow 7 Lake, Orange 4 Lake, and Red 28 Lake; the calcium lakes of D&C Red Nos. 7, 11, 31 and 34, the barium lake of D&C Red No. 12, the strontium lake D&C Red No. 13, the aluminum lakes of FD&C Yellow No. 5, of FD&C Yellow No. 6, of FD&C No. 40, of D&C Red Nos. 21, 22, 27, and 28, of FD&C Blue No. 1, of D&C Orange No. 5, of D&C Yellow No. 10, the zirconium lake of D&C Red No. 33; CROMOPHTAL® Yellow 131 AK (Ciba Specialty Chemicals), SUNFAST® Magenta 122 (Sun Chemical) and SUNFAST® Blue 15:3 (Sun Chemical), iron oxides, calcium carbonate, calcium phosphate, aluminum hydroxide, calcium sulfate, kaolin, ferric ammonium ferrocyanide, magnesium carbonate, carmine, barium sulfate, mica, bismuth oxychloride, zinc stearate, manganese violet, chromium oxide, titanium dioxide, silicon dioxide, black titanium dioxide, titanium dioxide nanoparticles, zinc oxide, aluminum oxide, tin oxide, calcium oxide, magnesium oxide, barium oxide, ultramarine blue, bismuth citrate, and white minerals such as hydroxyapatite, and Zircon (zirconium silicate), and carbon black particles.
In one embodiment, the white colorant of the polymer-coated white colorant is a white pigment selected from the group consisting of white mineral particles (e.g., calcium phosphate in various structural forms and hydroxyapatite), white metal oxides (e.g., titanium dioxide, titanium dioxide nanoparticles, and zinc oxide), silicon dioxide, zirconium silicate, to name a few. In some embodiments, white mineral particles can comprise a non-toxic mineral or salt that can impart a white color, for example, calcium phosphate in various structural forms, including tetracalcium phosphate, amorphous calcium phosphate, alpha-tricalcium phosphate, beta-tricalcium phosphate and hydroxyapatite. In another embodiment, white metal oxides include any metal oxide that provides a white color, such as titanium oxide, aluminum oxide, tin oxide, calcium oxide, magnesium oxide, barium oxide, zinc oxide or a combination thereof.
In a preferred embodiment, the white colorant of the polymer-coated white colorant is a white pigment selected from the group consisting of calcium phosphate, hydroxyapatite, titanium dioxide, titanium dioxide nanoparticles, zinc oxide, silicon dioxide, zirconium silicate, and a combination thereof. In a more preferred embodiment, the white colorant of the polymer-coated white colorant is a white pigment selected from the group consisting of titanium dioxide, titanium dioxide nanoparticles, zinc oxide, silicon dioxide, zirconium silicate, and a combination thereof. In a further preferred embodiment, the polymer-coated white colorant is a polymer-coated titanium dioxide
Pigments, by definition, are substantially insoluble colorants and therefore, are used in dispersed form. The pigment may be dispersed using a dispersant or a self-dispersing pigment may be used. When a dispersant is used to disperse the pigment, the dispersant may be any suitable dispersant known in the art, including, but not limited to, random or structured organic polymeric dispersants, as described below; protein dispersants, such as those described by Brueckmann et al. (U.S. Pat. No. 5,124,438); and peptide-based dispersants, such as those described by O'Brien et al (co-pending and commonly owned U.S. Patent Application Publication No. 2005-0054752). Preferred random organic polymeric dispersants include acrylic polymer and styrene-acrylic polymers. Most preferred are structured dispersants, which include AB, BAB and ABC block copolymers, branched polymers and graft polymers. Preferably the organic polymers comprise monomer units selected from the group consisting of acrylate, methacrylate, butyl methacrylate, 2-ethylhexyl methacrylate, benzyl methacrylate, phenoxyethyl acrylate, ethoxytriethyleneglycol methacrylate, polyethylene glycol methacrylate, polyethylene glycol acrylate, acrylic acid, methacrylic acid, methacrylamide, acrylamide, dimethylaminoethyl methacrylate, hydroxyethyl acrylate, and hydroxyethyl methacrylate, such as those described by Nigan (U.S. Patent Application Publication No. 2004/0232377). Some useful structured polymer dispersants are disclosed in U.S. Pat. No. 5,085,698; EP-A-0556649; and U.S. Pat. No. 5,231,131. Additionally, pigments may be dispersed using a surface active agent comprising lignin sulfonic acids and a polypeptide, as described by Cioca et al. in U.S. Pat. No. 4,494,994.
The pigment may optionally be surface-treated prior to coating with organic polymer. Common surface treatments include, but are not limited to alkyl silane, siloxane, methicone, and dimethicone. Surface treatment increases the range of polymers that can use to coat the pigment surface.
A self-dispersing pigment is a pigment that has been surface modified with chemically attached, dispersibility imparting groups to allow stable dispersion without a separate dispersant. For dispersion in an aqueous carrier medium, surface modification involves addition of hydrophilic groups and most typically ionizable hydrophilic groups. The self-dispersing pigment may be prepared by grafting a functional group or a molecule containing a functional group onto the surface of the pigment, by physical treatment (such as vacuum plasma), or by chemical treatment (for example, oxidation with ozone, hypochlorous acid or the like). A single type or a plurality of types of hydrophilic functional groups may be bonded to one pigment particle. Self-dispersing pigments are described, for example, in U.S. Pat. No. 5,571,311, U.S. Pat. No. 5,609,671, U.S. Pat. No. 5,968,243, U.S. Pat. No. 5,928,419, U.S. Pat. No. 6,323,257, U.S. Pat. No. 5,554,739, U.S. Pat. No. 5,672,198, U.S. Pat. No. 5,698,016, U.S. Pat. No. 5,718,746, U.S. Pat. No. 5,749,950, U.S. Pat. No. 5,803,959, U.S. Pat. No. 5,837,045, U.S. Pat. No. 5,846,307, U.S. Pat. No. 5,895,522, U.S. Pat. No. 5,922,118, U.S. Pat. No. 6,123,759, U.S. Pat. No. 6,221,142, U.S. Pat. No. 6,221,143, U.S. Pat. No. 6,281,267, U.S. Pat. No. 6,329,446, U.S. Pat. No. 6,332,919, U.S. Pat. No. 6,375,317, U.S. Pat. No. 6,287,374, U.S. Pat. No. 6,398,858, U.S. Pat. No. 6,402,825, U.S. Pat. No. 6,468,342, U.S. Pat. No. 6,503,311, U.S. Pat. No. 6,506,245, and U.S. Pat. No. 6,852,156.
Titanium dioxide nanoparticles are described in U.S. Pat. Nos. 5,451,390; 5,672,330; and 5,762,914. Titanium dioxide P25 is an example of a commercial product available from Degussa (Parsippany, N.J.). Other commercial suppliers of titanium dioxide nanoparticles include Kemira (Helsinki, Finland), Sachtleben (Duisburg, Germany) and Tayca (Osaka, Japan).
The titanium dioxide nanoparticles typically have an average particle size diameter of less than 100 nanometers (nm) as determined by dynamic light scattering which measures the particle size distribution of particles in liquid suspension. The nanoparticles are typically agglomerates which may range from about 3 nm to about 6000 nm. In one embodiment, the average particle size of the nanoparticle ranges from about 5 nm to about 100 nm.
Any process known in the art can be used to prepare such particles. The process may involve vapor phase oxidation of titanium halides or solution precipitation from soluble titanium complexes, provided that titanium dioxide nanoparticles are produced. A preferred process to prepare titanium dioxide nanoparticles is by injecting oxygen and titanium halide, preferably titanium tetrachloride, into a high-temperature reaction zone, typically ranging from 400 to 2000° C. Under the high temperature conditions present in the reaction zone, nanoparticles of titanium dioxide are formed having high surface area and a narrow size distribution. The energy source in the reactor may be any heating source such as a plasma torch.
Polymer-Coated White Colorants
The white colorant is coated with a polymeric coating such that peptides having an affinity for the polymer will bind to the polymeric coating. The polymeric coating may be formed from many different organic and biological polymers including, but not limited to cellulose acetate, polyacrylates, polymethacrylates, polymethylmethacrylates, polycarbonates, polystyrene, polypropylene, polyethylene terephthalate, polyurethanes, polypeptides, lignin, polysaccharides, modified polysaccharides, polyamides, polyimides, polyaramides, polyethers, silicones, methicones, dimethicones and copolymers, (e.g., block and graft copolymers) comprising at least one monomer from methacylates, acrylates or styrene. In a preferred embodiment, the polymeric coating is formed from a polymer selected from the group consisting of cellulose acetate, polysaccharides, modified polysaccharides, methacrylates and acrylates.
If a pigment dispersed with a polymeric dispersant, as described above, is used as the white colorant, the polymeric dispersant, may serve as the polymeric coating. The organic polymeric dispersants surrounding the pigment may optionally be cross linked by covalent or ionic bonds, generally after they have been applied to the pigment. Cross-linking increases the permanence and environmental-resistance of the polymeric coating. Any of the polymer dispersants described above may be used. For example, pigments dispersed with a polyacrylate-containing dispersant may be used in conjunction with a polyacrylate-binding peptide. Alternatively, the dispersed pigment may be coated with another polymer as described below.
For pigments and self-dispersing pigments and other particulate white colorants that are not typically used with a polymeric dispersant, the particles may be coated with a polymer using particle coating methods known in the art. Typically, methods used for coating particles are solution-based methods that rely on the application of a polymer solution onto the particle surface, followed by the removal of the solvent. For example, the particulate white colorant may be coated with a polymer by simply mixing the particles with a solution containing the polymer for a time sufficient to coat the particles and then removing the solvent. Additionally, the particulate white colorants may be coated with a polymer using spray coating techniques, such as those described by Guignon et al. (Drying Technol. 20:419-447 (2002)). Coatings may also be applied with a Wurster coater (see for example, Cardozo et al., U.S. Patent Application Publication No. 2006-0019860). The white colorants of the invention may also be coated with a polymer using an emulsification-solvent evaporation technique, as described by Rosca et al. (J. Control Release 99:271-280 (2004)). Additionally, white colorants may be coated with a polymer using the injector mixer method and apparatus described by Schurr (U.S. Pat. No. 4,430,001 and WO 97/007879). In the injector mixer method, small levels of additives are intensely mixed with powders by simultaneously atomizing the coating liquid and dispersing the particles in a gas injector. The method offers the advantages of low water use and very short contact time, which enables the coating of thermally sensitive materials at high temperatures.
Production of Binding Peptides
The binding peptides (tooth surface-binding peptides, polymer-binding peptides, and the peptide-based reagents) may be prepared using standard peptide synthesis methods, which are well known in the art (see for example Stewart et al., Solid Phase Peptide Synthesis, Pierce Chemical Co., Rockford, Ill., 1984; Bodanszky, Principles of Peptide Synthesis, Springer-Verlag, New York, 1984; and Pennington et al., Peptide Synthesis Protocols, Humana Press, Totowa, N.J., 1994). Additionally, many companies offer custom peptide synthesis services.
Alternatively, the peptides described herein may be prepared using recombinant DNA and molecular cloning techniques. Genes encoding the peptides may be produced in heterologous host cells, particularly in the cells of microbial hosts.
Preferred heterologous host cells for expression of the peptides of the present invention are microbial hosts that can be found broadly within the fungal or bacterial families and which grow over a wide range of temperature, pH values, and solvent tolerances. Because transcription, translation, and the protein biosynthetic apparatus are the same irrespective of the cellular feedstock, functional genes are expressed irrespective of carbon feedstock used to generate cellular biomass. Examples of host strains include, but are not limited to, fungal or yeast species such as Aspergillus, Trichoderma, Saccharomyces, Pichia, Candida, Yarrowia, Hansenula, or bacterial species such as Salmonella, Bacillus, Acinetobacter, Rhodococcus, Streptomyces, Escherichia, Pseudomonas, Methylomonas, Methylobacter, Alcaligenes, Synechocystis, Anabaena, Thiobacillus, Methanobacterium and Klebsiella. 
A variety of expression systems can be used to produce the peptides of the present invention. Such vectors include, but are not limited to chromosomal, episomal and virus-derived vectors, e.g., vectors derived from bacterial plasmids, from bacteriophage, from transposons, from insertion elements, from yeast episomes, from viruses such as baculoviruses, retroviruses and vectors derived from combinations thereof such as those derived from plasmid and bacteriophage genetic elements, such as cosmids and phagemids. The expression system constructs may contain regulatory regions that regulate as well as engender expression. In general, any system or vector suitable to maintain, propagate or express polynucleotide or polypeptide in a host cell may be used for expression in this regard. Microbial expression systems and expression vectors contain regulatory sequences that direct high level expression of foreign proteins relative to the growth of the host cell. Regulatory sequences are well known to those skilled in the art and examples include, but are not limited to, those which cause the expression of a gene to be turned on or off in response to a chemical or physical stimulus, including the presence of regulatory elements in the vector, for example, enhancer sequences. Any of these could be used to construct chimeric genes for production of the any of the binding peptides of the present invention. These chimeric genes could then be introduced into appropriate microorganisms via transformation to provide high level expression of the peptides.
Vectors or cassettes useful for the transformation of suitable host cells are well known in the art. Typically the vector or cassette contains sequences directing transcription and translation of the relevant gene, one or more selectable markers, and sequences allowing autonomous replication or chromosomal integration. Suitable vectors comprise a region 5′ of the gene, which harbors transcriptional initiation controls and a region 3′ of the DNA fragment which controls transcriptional termination. It is most preferred when both control regions are derived from genes homologous to the transformed host cell, although it is to be understood that such control regions need not be derived from the genes native to the specific species chosen as a production host. Selectable marker genes provide a phenotypic trait for selection of the transformed host cells such as tetracycline or ampicillin resistance in E. coli. 
Initiation control regions or promoters which are useful to drive expression of the chimeric gene in the desired host cell are numerous and familiar to those skilled in the art. Virtually any promoter capable of driving the gene is suitable for producing the binding peptides of the present invention including, but not limited to: CYC1, HIS3, GAL1, GAL10, ADH1, PGK, PHO5, GAPDH, ADC1, TRP1, URA3, LEU2, ENO, TPI (useful for expression in Saccharomyces); AOX1 (useful for expression in Pichia); and lac, pBAD, tet, trp, IPL, IPR, T7, tac, and trc (useful for expression in Escherichia coli) as well as the amy, apr, npr promoters and various phage promoters useful for expression in Bacillus. 
Termination control regions may also be derived from various genes native to the preferred hosts. Optionally, a termination site may be unnecessary, however, it is most preferred if included.
The vector containing the appropriate DNA sequence, as well as an appropriate promoter or control sequence, may be employed to transform an appropriate host to permit the host to express the peptide of the present invention. Cell-free translation systems can also be employed to produce such peptides using RNAs derived from the DNA constructs of the present invention. Optionally it may be desired to produce the instant gene product as a secretion product of the transformed host. Secretion of desired proteins into the growth media has the advantages of simplified and less costly purification procedures. It is well known in the art that secretion signal sequences are often useful in facilitating the active transport of expressible proteins across cell membranes. The creation of a transformed host capable of secretion may be accomplished by the incorporation of a DNA sequence that codes for a secretion signal which is functional in the production host. Methods for choosing appropriate signal sequences are well known in the art (see for example EP 546049 and WO 9324631). The secretion signal DNA or facilitator may be located between the expression-controlling DNA and the instant gene or gene fragment, and in the same reading frame with the latter.
Peptide-Based Reagents Comprising at Least One Tooth-Binding Peptide and at Least One Polymer-Binding Peptide
Peptide-based reagents are formed by coupling at least one tooth-binding peptide to at least one polymer-binding peptide, either directly or through one or more spacers. The part of the reagent comprising at least one tooth-binding peptide binds strongly to a tooth surface, while the part of the reagent comprising at least one polymer-binding peptide binds strongly to a polymer-coated white colorant, thereby attaching the polymer-coated white colorant to the tooth surface. It is understood that one of skill in the art will select polymer-binding peptides that binds with high affinity to the polymer used to coat the white colorant.
In one embodiment, the peptide-based reagent comprises a peptide having the general structure:

[(TBP)m-(PBP)n]x, wherein

    
    
        a) TBP is a tooth-binding peptide;
        b) PBP is a polymer-binding peptide; and
        c) m, n, and x independently range from 1 to about 10.
    
    


In another embodiment of the peptide described above, m and n are preferably from 1 to about 5, and x may be 1 to about 10.
In another embodiment, the peptide-based reagent comprises a peptide having the general structure:

[[(TBP)y-Sq]m-[(PBP)z-Sr]n]p, wherein

    
    
        a) TBP is a tooth-binding peptide;
        b) PBP is a polymer-binding peptide;
        c) S is a spacer;
        d) m, n, y and z independently range from 1 to about 10;
        e) p is from 1 to 5; and
        f) q and r are each independently 0 or 1, provided that both q and r may not be 0.
    
    


In a preferred embodiment, m and n independently range from 1 to about 3, and y and z independently range from 1 to about 5.
An example of a peptide-based reagent (peptide conjugate) is provided as SEQ ID NO: 116.
The coupling interaction may be a covalent bond or a non-covalent interaction, such as hydrogen bonding, electrostatic interaction, hydrophobic interaction, or Van der Waals interaction. In the case of a non-covalent interaction, the peptide-based reagents may be prepared by mixing at least one tooth-binding peptide and at least one polymer-binding peptide and the optional spacer (if used) and allowing sufficient time for the interaction to occur. The unbound materials may be separated from the resulting peptide-based reagents using methods known in the art, for example, gel permeation chromatography.
The peptide-based reagents may also be prepared by covalently attaching at least one tooth-binding peptide to at least one polymer-binding peptide, either directly or through a spacer. In this embodiment, the polymer-coated white colorant is delivered to the tooth surface using the non-covalent interact between the polymer-binding peptide and the polymer-coated white colorant.
Any known peptide or protein conjugation chemistry may be used to form the peptide-based reagents of the invention. Conjugation chemistries are well-known in the art (see for example, Hermanson, Bioconjugate Techniques, Academic Press, New York (1996)). Suitable coupling agents include, but are not limited to, carbodiimide coupling agents, diacid chlorides, diisocyanates and other difunctional coupling reagents that are reactive toward terminal amine and/or carboxylic acid groups on the peptides. The preferred coupling agents are carbodiimide coupling agents, such as 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and N,N′-dicyclohexyl-carbodiimide (DCC), which may be used to activate carboxylic acid groups. Additionally, it may be necessary to protect reactive amine or carboxylic acid groups on the peptides to produce the desired structure for the peptide-based reagent. The use of protecting groups for amino acids, such as t-butyloxycarbonyl (t-Boc), are well known in the art (see for example Stewart et al., supra; Bodanszky, supra; and Pennington et al., supra).
The peptide constructs described herein may be prepared using recombinant DNA and molecular cloning techniques described herein. In a preferred embodiment, the peptide-based reagents comprising at least one tooth-binding peptide and at least one polymer-binding peptide (optionally comprising one or more spacers) are prepared using recombinant DNA and molecular cloning techniques described herein.
It may also be desirable to couple the tooth-binding peptide to the polymer-binding peptide via a spacer to form the peptide-based reagent. The spacer serves to separate the binding peptide sequences to ensure that the binding affinity of the individual peptides is not adversely affected by the coupling. The spacer may also provide other desirable properties such as hydrophilicity, hydrophobicity, or a means for cleaving the peptide sequences to facilitate removal of the whitening agent.
The spacer, also referred to herein as the “organic spacer”, may be any of a variety of molecules, such as alkyl chains, phenyl compounds, ethylene glycol, amides, esters and the like. Preferred spacers are hydrophilic and have a chain length from 1 to about 100 atoms, more preferably, from 2 to about 30 atoms. Examples of spacers may include, but are not limited to, ethanolamine, ethylene glycol, polyethylene with a chain length of 6 carbon atoms, polyethylene glycol with 3 to 6 repeating units, phenoxyethanol, propanolamide, butylene glycol, butyleneglycolamide, propyl phenyl chains, ethyl alkyl chains, propyl alkyl chains, hexyl alkyl chains, steryl alkyl chains, cetyl alkyl chains, palmitoyl alkyl chains, and peptide spacers.
The spacer may be covalently attached to the tooth-binding and polymer-binding peptide sequences using any of the coupling chemistries described above. In order to facilitate incorporation of the spacer, a bifunctional cross-linking agent that contains a spacer and reactive groups at both ends for coupling to the peptides may be used.
Suitable bifunctional cross-linking agents are well known in the art and include, but are not limited to diamines, such a as 1,6-diaminohexane; dialdehydes, such as glutaraldehyde; bis N-hydroxysuccinimide esters, such as ethylene glycol-bis(succinic acid N-hydroxysuccinimide ester), disuccinimidyl glutarate, disuccinimidyl suberate, and ethylene glycol-bis(succinimidylsuccinate); diisocyanates, such as hexamethylenediisocyanate; bis oxiranes, such as 1,4 butanediyl diglycidyl ether; dicarboxylic acids, such as succinyldisalicylate; and the like. Heterobifunctional cross-linking agents, which contain a different reactive group at each end, may also be used.
Examples of heterobifunctional cross-linking agents include, but are not limited to compounds having the following structure:




where: R1 is H or a substituent group such as —SO3Na, —NO2, or —Br; and R2 is a spacer such as —CH2CH2 (ethyl), —(CH2)3 (propyl), or —(CH2)3C6H5 (propyl phenyl). An example of such a heterobifunctional cross-linking agent is 3-maleimidopropionic acid N-hydroxysuccinimide ester. The N-hydroxysuccinimide ester group of these reagents reacts with amine groups on one peptide, while the maleimide group reacts with thiol groups present on the other peptide. A thiol group may be incorporated into the peptide by adding at least one cysteine group to at least one end of the binding peptide sequence (i.e., the C-terminal end or N-terminal end). Several spacer amino acid residues, such as glycine, may be incorporated between the binding peptide sequence and the terminal cysteine to separate the reacting thiol group from the binding sequence. Moreover, at least one lysine residue may be added to at least one end of the binding peptide sequence, i.e., the C-terminal end or the N-terminal end, to provide an amine group for coupling.

Additionally, the spacer may be a peptide spacer. The preferred peptide spacers comprise the amino acids selected from the group consisting of proline, lysine, glycine, alanine, serine, and mixtures thereof. In addition, the peptide spacer may contain a specific enzyme cleavage site, such as the protease Caspase 3 site, given by SEQ ID NO: 109, which allows for the enzymatic removal of the polymer-coated white colorant from the tooth surface. Examples of peptides spacers include, but are not limited to the sequences given by SEQ ID NOs: 110-115. These peptide spacers may be linked to the binding peptide sequences by any method known in the art. For example, the entire triblock peptide-based reagent may be prepared using the standard peptide synthesis methods described, supra. In addition, the binding peptides and peptide spacer block may be combined using carbodiimide coupling agents, diacid chlorides, diisocyanates and other difunctional coupling reagents that are reactive to terminal amine and/or carboxylic acid groups on the peptides, as described above. Alternatively, the entire triblock peptide-based reagent may be prepared using the recombinant DNA and molecular cloning techniques described supra. The spacer may also be a combination of a peptide spacer and an organic spacer molecule, which may be prepared using the methods described above.
It may also be desirable to have multiple copies of the tooth-binding peptide and the polymer-binding peptide coupled together to enhance the interaction between the peptide-based reagent, the tooth surface, and the polymer-coated white colorant. Either multiple copies of the same tooth-binding peptide and polymer-binding peptide or a combination of different tooth-binding peptides and polymer-binding peptides may be used. The multi-copy peptide-based reagents may comprise various spacers as described above.
In another embodiment, the peptide-based reagent comprises a spacer (S) separating the tooth-binding peptide from the polymer-binding peptide, as described above. Multiple copies of the tooth-binding peptide and the polymer-binding peptide may also be used and the multiple copies of the tooth-binding peptide and the polymer-binding peptide may be separated from themselves and from each other by spacers. In this embodiment, the peptide-based reagent is a “triblock” composition comprising at least one tooth-binding peptide, at least one spacer, and at least one polymer-binding peptides, having the general structure [[(TBP)y-Sq]m-[(PBP)z-Sr]n]p, where n, m, y, and z independently range from 1 to about 10, p is from 1 to about 5, and where q and r are each independently 0 or 1, provided that both q and r are not 0. Preferably, m and n independently range from 1 to about 3, and y and z range from 1 to about 5.
It should be understood that as used herein “TBP” and “PBP” are generic designations and are not meant to refer to a single tooth-binding peptide or polymer-binding peptide, respectively. Where y or z as used above, is greater than 1, it is well within the scope of the invention to provide for the situation where a series of tooth-binding peptides of same or different sequences and polymer-binding peptides of same or different sequences may form a part of the composition. Additionally, “S” is a generic term and is not meant to refer to a single spacer. Where m and n, as used above for the triblock compositions, are greater than 1, it is well within the scope of the invention to provide for the situation where a series of same or different spacers may form a part of the composition. It should also be understood that these structures do not necessarily represent a covalent bond between the peptides and the optional spacer. As described above, the coupling interaction between the peptides and the optional spacer may be either covalent or non-covalent. In a preferred embodiment, the peptide-based reagent is a linear, recombinantly produced peptide comprising at least one tooth-binding peptide, at least one polymer-binding peptide, and optionally one or more peptide spacers.
Peptide-Based Reagents Comprising at Least One Tooth-Binding Peptide Coupled to a Polymer-Coated White Colorant
In another aspect, the peptide based reagent may comprise at least one tooth-binding peptide coupled directly to a polymer-coated white colorant. The tooth-binding peptide is used to couple the polymer-coated white colorant to teeth. The peptide-based reagent may optionally be comprised of one or more spacers. Suitable spacers and suitable coupling chemistry are described above.
In one embodiment, a peptide-based reagent comprising the following structure is also provided:

(TBP)k-PCWC, wherein

    
    
        a) TBP is a tooth-binding peptide;
        b) PCWC is a polymer-coated white colorant; and
        c) k ranges from 1 to about 10,000.
    
    


In another embodiment, the polymer-coated white colorant may be coupled to a tooth-binding peptide through one or more spacers. As such, a peptide-based reagent comprising the following structure is also provided:

[(TBP)v-L]k-PCWC, wherein

    
    
        a) TBP is a tooth-binding peptide;
        b) PCWC is a polymer-coated white colorant;
        c) L is a spacer;
        d) v ranges from 1 to about 50; and
        e) k ranges from 1 to about 10,000.
    
    


It may also be desirable to couple the tooth-binding peptide to the polymer-coated white colorant (PCWC) via a spacer L to form a tooth whitening reagent, provided that spacer L does not adversely affect the binding affinity of the tooth-binding peptide. The spacer L may also provide other desirable properties such as hydrophilicity, hydrophobicity, or a means for cleaving the peptide sequences to facilitate removal of the whitening agent. The spacer L can be an organic spacer or a peptide spacer, which are similarly defined previously for spacer S.
In another embodiment, a peptide-based reagent is also provided comprising a spacer L separating the tooth-binding peptide from the polymer-coated white colorant, as described above. Multiple copies of the tooth-binding peptides may also be used, and the multiple copies of the tooth-binding peptides and the polymer-coated white colorant may be separated from themselves and from each other by different spacers.
Oral Care Compositions
The present invention includes oral care compositions comprising an effective amount of one or more of the present peptide-based reagents and an effective amount of at least one polymer-coated white colorant. As used here, the term “effective amount” is that amount of at least one peptide-based reagent and the amount of at least one polymer-coated white colorant incorporated into the oral care composition to achieve the desired improvement of whiter-appearing teeth.
Methods to apply polymer coating to a particle, such as a white colorant, are well known in the art. Examples of white colorants may include, but are not limited to, white pigments such as titanium dioxide, titanium dioxide nanoparticles and zinc oxide, white minerals such as hydroxyapatite, Zircon (zirconium silicate), and mixtures thereof. However, it may be desirable to further include at least one non-white pigment in an oral care composition to achieve the desired coloration.
The oral care compositions of the invention may be in the form of powder, paste, gel, liquid, ointment, or tablet. Exemplary oral care compositions include, but are not limited to, toothpaste, dental cream, gel or tooth powder, mouth wash, breath freshener, and dental floss. The oral care compositions comprise an effective amount of a peptide-based reagent and a polymer-coated white colorant in an orally acceptable carrier medium. An effective amount of a peptide-based reagent for use in an oral care composition may vary depending on the type of product. Typically, the effective amount of the peptide-based reagent is a proportion from about 0.01% to about 90% by weight relative to the total weight of the composition. Additionally, a mixture of different peptide-based reagents having affinity for different polymer-coated white colorants may be used in the composition. The peptide-based reagents in the mixture need to be chosen so that there is no interaction between the peptides that mitigates the whitening effect. Suitable mixtures of peptide-based reagents may be determined by one skilled in the art using routine experimentation. If a mixture of peptide-based reagents is used in the composition, the total concentration of the reagents is about 0.001% to about 90% by weight relative to the total weight of the composition.
Components of an orally-acceptable carrier medium are described by White et al. in U.S. Pat. No. 6,740,311; Lawler et al. in U.S. Pat. No. 6,706,256; Fuglsang et al. in U.S. Pat. No. 6,264,925; and Ibrahim et al., U.S. App. Pub. No. 2005/0069501, all of which are incorporated herein by reference. For example, the oral care composition may comprise one or more of the following: abrasives, surfactants, antioxidants, chelating agents, fluoride sources, thickening agents, buffering agents, solvents, humectants, carriers, bulking agents, and oral benefit agents, such as enzymes, anti-plaque agents, anti-staining agents, anti-microbial agents, anti-caries agents, anti-inflammatory agents, desensitizing agents, sweetening agents, flavoring agents, breath-freshening agents, coolants, nutrients, and salivating agents.
In another embodiment, the peptide-based reagent is used to detect the presence of a particular surface on teeth (e.g., a diagnostic application). For example, the peptide-based reagent may be used to detect the presence of a pellicle coating on teeth immediately after an abrasive cleaning/polishing procedure (e.g., a dental office cleaning/polishing procedure).
Methods for Whitening a Tooth Surface
The peptide-based reagents may be used in conjunction with a polymer-coated white colorant to whiten teeth. The tooth-binding peptide block of the peptide-based reagent has an affinity for a tooth surface, while the polymer-binding peptide block has an affinity for a polymer-coated white colorant (such as a white pigment). The peptide-based reagent may be present in the same composition as the polymer-coated white colorant, or the peptide-based reagent and the polymer-coated white colorant may be present in two different compositions until use. In one embodiment, an oral care composition comprising at least one peptide-based reagent and at least one polymer-coated white colorant is applied to a tooth surface for a time sufficient for the peptide-based reagent, which is non-covalently coupled to the polymer-coated-white colorant via the polymer-binding peptide block, to bind to the tooth surface. In another embodiment, at least one polymer-coated white colorant is applied to a tooth surface prior to the application of a composition comprising at least one peptide-based reagent. In another embodiment, a composition comprising at least one peptide-based reagent is applied to the tooth surface prior to the application of the polymer-coated white colorant. In another embodiment, at least one polymer-coated white colorant and a composition comprising at least one peptide-based reagent are applied to the tooth surface concomitantly, that is together. Optionally, the composition comprising the peptide-based reagent may be reapplied to the tooth surface after the application of the polymer-coated white colorant and the initial application of the composition comprising the peptide-based reagent. Additionally, a composition comprising a separate polymeric sealant, such as the orally-acceptable GANTREZ® copolymer (copolymers of methyl vinyl ether and maleic anhydride used in commercially-available oral care compositions and available from International Specialty Products, Wayne, N.J.) may be applied to the tooth surface after the application of a composition comprising the polymer-coated white colorant and peptide-based reagent.
In one embodiment, at least one polymer-coated white colorant is applied to the surface of teeth for a time sufficient for the polymer-coated white colorant to bind to the teeth, typically between about 5 seconds to about 60 minutes. Optionally, the teeth may be rinsed to remove the polymer-coated white colorant that has not bound to the teeth. Then, a composition comprising a peptide-based reagent is applied for a time sufficient for the peptide-based reagent to bind to the teeth and the polymer-coated white colorant, typically between about 5 seconds to about 60 minutes. The composition comprising the peptide-based reagent may be rinsed from the teeth or left on the teeth.
In another embodiment, a composition comprising a peptide-based reagent is applied to the teeth for a time sufficient for the tooth-binding peptide block of the peptide-based reagent to bind to the teeth, typically between about 5 seconds to about 60 minutes. Optionally, the teeth may be rinsed to remove the composition that has not bound to the teeth. Then, at least one polymer coated white colorant may be applied to the teeth for a time sufficient for the at least one polymer coated white colorant to bind to the polymer-binding block of the peptide-based reagent, typically between about 5 seconds to about 60 minutes. The unbound polymer-coated white colorant may be rinsed from the teeth or left on the teeth.
In another embodiment, at least one polymer coated white colorant and a composition comprising at least one peptide-based reagent are applied to the teeth concomitantly (or together) for a time sufficient for the peptide-based reagent to bind to teeth and the polymer-coated white colorant, typically between about 5 seconds to about 60 minutes. Optionally, the teeth may be rinsed to remove the unbound polymer coated white colorant and the composition comprising the peptide-based reagent from the teeth.
In another embodiment, at least one polymer coated white colorant is provided as part of a composition comprising at least one peptide-based reagent, for example a tooth whitening composition. The composition comprising the polymer-coated white colorant and the peptide-based reagent is applied to the teeth for a time sufficient for the peptide-based reagent, which is coupled to the polymer-coated white colorant through the polymer-binding peptide block, to bind to the surface of teeth, typically between about 5 seconds to about 60 minutes. The composition comprising the polymer-coated white colorant and the peptide-based reagent may be rinsed from the teeth or left on the teeth.
In any of the methods described above, the composition comprising a peptide-based reagent may be optionally reapplied to the teeth after the application of the polymer coated white colorant and the initial application of the composition comprising the peptide-based reagent in order to further enhance the durability of the colorant.
Additionally, in any of the methods described above, a composition comprising an additional polymeric sealant may be optionally applied to the teeth after the application of the polymer-coated white colorant and the composition comprising a peptide-based reagent in order to further enhance the durability of the colorant. The composition comprising the additional polymeric sealant may be an aqueous solution or an oral care composition, such as a toothpaste or mouthwash, comprising the polymeric sealant (such as GANTREZ® copolymers). Typically, the polymeric sealant is present in the composition at a concentration of about 0.25% to about 10% by weight relative to the total weight of the composition. Additional polymeric sealants well known in the art of personal care products may be used and may include, but are not limited to, poly(allylamine), acrylates, acrylate copolymers, polyurethanes, carbomers, methicones, amodimethicones, polyethylenene glycol, beeswax, siloxanes, PLASDONE® and POLYPLASDONE® (linear and cross linked polyvinylpyrrolidone homopolymers, respectively; available for International Specialty Products), and combinations thereof. The choice of polymeric sealant may be selected based on the particular white colorant and the peptide-based reagent used. The optimum type and amount of additional polymeric sealant may be readily determined by one skilled in the art using routine experimentation.
EXAMPLES
The present invention is further defined in the following Examples. It should be understood that these Examples, while indicating preferred embodiments of the invention, are given by way of illustration only. From the above discussion and these Examples, one skilled in the art can ascertain the essential characteristics of this invention, and without departing from the spirit and scope thereof, can make various changes and modifications of the invention to adapt it to various uses and conditions.
The meaning of abbreviations is as follows: “min” means minute(s), “sec” means second(s), “h” means hour(s), “μL” means microliter(s), “mL” means milliliter(s), “L” means liter(s), “nm” means nanometer(s), “mm” means millimeter(s), “cm” means centimeter(s), “μm” means micrometer(s), “mM” means millimolar, “M” means molar, “mmol” means millimole(s), “μmole” means micromole(s), “g” means gram(s), “μg” means microgram(s), “ng” means nanograms, “mg” means milligram(s), “g” means the gravitation constant, “rpm” means revolution(s) per minute, “pfu” means plaque forming unit, “BSA” means bovine serum albumin, “ELISA” means enzyme linked immunosorbent assay, “IPTG” means isopropyl β-D-thiogalactopyranoside, “A” means absorbance, “A450” means the absorbance measured at a wavelength of 450 nm, “OD600” means the optical density measured at 600 nanometers, “TBS” means Tris-buffered saline, “TBST-X” means Tris-buffered saline containing TWEEN® 20 where “X” is the weight percent of TWEEN® 20, “Xgal” means 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside, “SEM” means standard error of the mean, “MW” means molecular weight, “Mw” means weight-average molecular weight, “vol %” means volume percent, “wt %” means weight percent, and “RCF” means relative centrifugal field.
General Methods
Standard recombinant DNA and molecular cloning techniques used herein are well known in the art and are described by Sambrook, J. and Russell, D., Molecular Cloning: A Laboratory Manual, Third Edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (2001); and by Silhavy, T. J., Bennan, M. L. and Enquist, L. W., Experiments with Gene Fusions, Cold Spring Harbor Laboratory Cold Press Spring Harbor, N.Y. (1984); and by Ausubel, F. M. et. al., Short Protocols in Molecular Biology, 5th Ed. Current Protocols and John Wiley and Sons, Inc., N.Y., 2002.
Materials and methods suitable for the maintenance and growth of bacterial cultures are also well known in the art. Techniques suitable for use in the following Examples may be found in Manual of Methods for General Bacteriology, Phillipp Gerhardt, R. G. E. Murray, Ralph N. Costilow, Eugene W. Nester, Willis A. Wood, Noel R. Krieg and G. Briggs Phillips, eds., American Society for Microbiology, Washington, D.C., 1994, or by Thomas D. Brock in Biotechnology: A Textbook of Industrial Microbiology, Second Edition, Sinauer Associates, Inc., Sunderland, Mass., 1989. All reagents, restriction enzymes and materials used for the growth and maintenance of bacterial cells were obtained from Aldrich Chemicals (Milwaukee, Wis.), BD Diagnostic Systems (Sparks, Md.), Life Technologies (Rockville, Md.), or Sigma-Aldrich Chemical Company (St. Louis, Mo.), unless otherwise specified.
Example 1
Selection of Tooth Pellicle-Binding Peptides Using Standard Biopanning
The purpose of this Example was to identify phage peptides that bind tooth pellicle using standard phage display biopanning.
The compressed HAP disks (Hydroxyapatite disk, 3 mm diameter) were used to form the pellicles by incubating the disks inside a human mouth for 1.5 hours followed by TBS rinse. The disks were then incubated in SUPERBLOCK® blocking buffer (Pierce Chemical Company, Rockford, Ill.; Prod. #37535) for 1 hour at room temperature, followed by 3 washes with TBST (TBS in 0.05% TWEEN® 20). Libraries of phage containing random peptide inserts (1011 pfu) from 7 to 20 amino acids were added to each tube. After 60 minutes of incubation at room temperature and shaking at 50 rpm, unbound phages were removed by aspirating the liquid out of each well followed by 6 washes with 1.0 mL TBS containing the detergent TWEEN® 20 (TBST) at a final concentration of 0.05%.
The sample disks were then transferred to clean tubes and 200 μL of elution buffer consisting of 1 mg/mL BSA in 0.2 M glycine-HCl, pH 2.2, was added to each well and incubated for 10 min to elute the bound phages. Then, 32 μL of neutralization buffer consisting of 1 M Tris-HCl, pH 9.2, was added to each well. The phage particles, which were in the elution buffer as well as on the sample disks, were amplified by incubating with diluted E. coli ER2738 cells, from an overnight culture diluted 1:100 in LB medium, at 37° C. for 4.5 h. After this time, the cell culture was centrifuged for 30 seconds and the upper 80% of the supernatant was transferred to a fresh tube, 1⁄6 volume of PEG/NaCl (20% polyethylene glycol-800, 2.5 M sodium chloride) was added, and the phage was allowed to precipitate overnight at 4° C. The precipitate was collected by centrifugation at 10,000×g at 4° C. and the resulting pellet was resuspended in 1 mL of TBS. This was the first round of amplified stock. The amplified first round phage stock was then tittered according to the standard protocol. For the 2nd, 3rd and 4th round of biopanning, more than 2×1011 pfu of phage stock from the previous round was used. The biopanning process was repeated for 2 additional rounds under the same conditions as described above. The same biopanning condition was used for the 4th round, except the washing solution was TBS with 0.5% TWEEN® 20 instead of 0.05% TWEEN® 20.
After the 4th round of biopanning, 95 random single phage plaque lysates were prepared following the manufacture's instructions (New England BioLabs) and the single stranded phage genomic DNA was purified using the QIAprep Spin M13 Kit (Qiagen, Valencia, Calif.) and sequenced at the DuPont Sequencing Facility using −96 gIII sequencing primer (5′-CCCTCATAGTTAGCGTAACG-3′), given as SEQ ID NO: 68. The displayed peptide is located immediately after the signal peptide of gene III. Based on the peptide sequences, 20 phage candidates were selected for further pellicle-binding analysis.
Example 2
Characterization of Tooth Pellicle-Binding Candidates on Pellicle Surface
A total of 20 selected phage candidates (Example 1) were used in a phage ELISA experiment. Purified phage lysates were used for binding to pellicle-coated HAP disks using an anti-M13 phage antibody conjugated to horseradish-peroxidase, followed by the addition of chromogenic agent TMB (3,3′,5,5′-tetramethylbenzidine), obtained from Pierce Biotechnology (Item #34021; Rockford, Ill.). The plates were read at A450 nm.
For each phage candidate to be tested, the pellicle-coated HAP disks (3 mm diameter) was incubated for 1 h at room temperature with 200 μL of blocking buffer, consisting of 2% non-fat dry milk (Schleicher & Schuell, Inc.) in TBS. The blocking buffer was removed by aspirating the liquid out of each tube. The tube was rinsed 6 times with wash buffer consisting of TBST-0.05%. The wells were filled with 200 μL of TBST-0.5% containing 1 mg/mL BSA (bovine serum albumin) and then 10 μL (over 1010 pfu) of purified phage stock was added. The samples were incubated at room temperature for 60 min with slow shaking. The non-binding phage were removed by washing 6 times with TBST-0.5%. Then, 100 μL of horseradish peroxidase/anti-M13 antibody conjugate (Amersham USA, Piscataway, N.J.), diluted 1:500 in the blocking buffer, was added and incubated for 1 hour at room temperature. The conjugate solution was removed and was washed 6 times with TBST-0.5%. TMB (3,3′,5,5′-tetramethylbenzidine) substrate (200 μL), obtained from Pierce Biotechnology (Rockford, Ill.) was added to each well and the color was allowed to develop for 5 to 30 min, typically for 10 min, at room temperature. Then, stop solution (200 μL of 2 M H2SO4) was added to each well and the solution was transferred to a 96-well plate and the A450 was measured using a microplate spectrophotometer (Molecular Devices, Sunnyvale, Calif.). The resulting absorbance values are given in Table 1.







TABLE 1







Amino Acid Sequences of


Pellicle-Binding Peptides












SEQ ID
O.D.


Phage ID
Amino Acids Sequence
NO.
at 450 nm













Control
No phage
—
0.218


 


Pell 1
AHPESLGIKYALDGNSDPHA
69
0.739


 


Pell 2
ASVSNYPPIHHLATSNTTVN
70
0.75


 


Pell 3
DECMEPLNAAHCWR
71
0.49


 


Pell 4
DECMHGSDVEFCTS
72
0.664


 


Pell 5
DLCSMQMMNTGCHY
73
0.83


 


Pell 6
DLCSSPSTWGSCIR
74
0.735


 


Pell 7
DPNESNYENATTVSQPTRHL
75
0.831


 


Pell 8
EPTHPTMRAQMHQSLRSSSP
76
0.712


 


Pell 9
GNTDTTPPNAVMEPTVQHKW
77
0.755


 


Pell 10
NGPDMVQSVGKHKNS
78
0.729


 


Pell 11
NGPEVRQIPANFEKL
79
0.607


 


Pell 12
NNTSADNPPETDSKHHLSMS
80
0.521


 


Pell 13
NNTWPEGAGHTMPSTNIRQA
81
0.598


 


Pell 14
NPTATPHMKDPMHSNAHSSA
82
0.7


 


Pell 15
NPTDHIPANSTNSRVSKGNT
83
0.567


 


Pell 16
NPTDSTHMMHARNHE
84
0.578


 


Pell 17
QHCITERLHPPCTK
85
0.614


 


Pell 18
TPCAPASFNPHCSR
86
0.416


 


Pell 19
TPCATYPHFSGCRA
87
0.731


 


Pell 20
WCTDFCTRSTPTSTSRSTTS
88
0.715









Example 3
Selection of Tooth Enamel-Binding Peptides Using Standard Biopanning
The purpose of this example was to identify phage peptides that bind tooth enamel using standard phage display biopanning.
The unpolished bovine enamel blocks from incisors (3 mm squares) and polished bovine enamel disks from the incisors (3 mm diameter disks) were embedded in wax to form a well with only the intended surfaces exposed. The enamel surfaces were then incubated in SUPERBLOCK® blocking buffer (Pierce Chemical Company, Rockford, Ill.; Prod. #37535) for 1 hour at room temperature, followed by 3 washes with TBST (TBS in 0.05% TWEEN® 20). Libraries of phage containing random peptide inserts (1011 pfu) from 7 to 20 amino acids were added to the enamel well for 10 minutes, pre-absorption, to titrate the wax surface, unbound phages were removed by aspirating the liquid out of each well. Then, 100 μL of the same phage library (1011 pfu) was added to the enamel well for 60 min incubation at room temperature with slow 50 rpm shaking, followed by 6 washes with 1.0 mL TBS containing the detergent TWEEN® 20 (TBST) at a final concentration of 0.05%.
The enamel blocks (or polished disks) were then cut out of the wax well and transferred to a clean tube and 200 μL of elution buffer consisting of 1 mg/mL BSA in 0.2 M glycine-HCl, pH 2.2, was added to each well and incubated for 10 min to elute the bound phages. Then, 32 μL of neutralization buffer consisting of 1 M Tris-HCl, pH 9.2, was added to each tube. The phage particles, which were in the elution buffer as well as on the enamel blocks, were amplified by incubating with diluted E. coli ER2738 cells, from an overnight culture diluted 1:100 in LB medium, at 37° C. for 4.5 h. After this time, the cell culture was centrifuged for 30 s and the upper 80% of the supernatant was transferred to a fresh tube, 1⁄6 volume of PEG/NaCl (20% polyethylene glycol-800, 2.5 M sodium chloride) was added, and the phage was allowed to precipitate overnight at 4° C. The precipitate was collected by centrifugation at 10,000×g at 4° C. and the resulting pellet was resuspended in 1 mL of TBS. This was the first round of amplified stock. The amplified first round phage stock was then tittered according to the standard protocol. For the 2nd round of biopanning, more than 2×1011 pfu of phage stock from the previous round was used. The biopanning process was repeated for 1 more rounds under the same conditions as described above. The same biopanning condition was used for the 3rd round, except the washing solution was TBS with 0.5% TWEEN® 20 instead of 0.05% TWEEN® 20.
After the 3rd round of biopanning, 95 random single phage plaque lysates were prepared following the manufacture's instructions (New England BioLabs) and the single stranded phage genomic DNA was purified using the QIAprep Spin M13 Kit (Qiagen, Valencia, Calif.) and sequenced at the DuPont Sequencing Facility using −96 gIII sequencing primer (5′-CCCTCATAGTTAGCGTAACG-3′), given as SEQ ID NO:68. The displayed peptide is located immediately after the signal peptide of gene III. Based on the peptide sequences, 20 phage candidates were selected for further pellicle binding analysis (Table 2). “BoEn” means bovine enamel and “BoEn P” means polished bovine enamel.







TABLE 2







Tooth Enamel-Binding Peptide Sequences











Phage ID
Amino Acid Sequence
SEQ ID NO:















BoEn P1
APPLKTYMQERELTMSQNKD
89



 



BoEn P2
EPPTRTRVNNHTVTVQAQQH
90



 



BoEn P3
GYCLRGDEPAVCSG
91



 



BoEn P4
LSSKDFGVTNTDQRTYDYTT
92



 



BoEn P5
NFCETQLDLSVCTV
93



 



BoEn P6
NTCQPTKNATPCSA
94



 



BoEn P7
PSEPERRDRNIAANAGRFNT
95



 



BoEn P8
THNMSHFPPSGHPKRTAT
96



 



BoEn P9
TTCPTMGTYHVCWL
97



 



BoEn P10
YCADHTPDPANPNKICGYSH
98



 



BoEn 1
AANPHTEWDRDAFQLAMPPK
99



 



BoEn 2
DLHPMDPSNKRPDNPSDLHT
100



 



BoEn 3
ESCVSNALMNQCIY
101



 



BoEn 4
HNKADSWDPDLPPHAGMSLG
102



 



BoEn 5
LNDQRKPGPPTMPTHSPAVG
103



 



BoEn 6
NTCATSPNSYTCSN
104



 



BoEn 7
SDCTAGLVPPLCAT
105



 



BoEn 8
TIESSQHSRTHQQNYGSTKT
106



 



BoEn 9
VGTMKQHPTTTQPPRVSATN
107



 



BoEn 10
YSETPNDQKPNPHYKVSGTK
108










Example 4
Characterization of Tooth Enamel-Binding Peptide Candidates on Enamel Surface
A total of 11 selected phage candidates (Example 3) were used in a phage ELISA experiment. Purified phage lysates were used for binding to the enamel blocks using an anti-M13 phage antibody conjugated to horseradish-peroxidase, followed by the addition of chromogenic agent TMB, obtained from Pierce Biotechnology (Rockford, Ill.). The plates were read at A450 nm.
For each phage candidate to be tested, the polished and unpolished enamel blocks were incubated for 1 h at room temperature with 200 μL of blocking buffer, consisting of 2% non-fat dry milk (Schleicher & Schuell, Inc.) in TBS. The blocking buffer was removed by aspirating the liquid out of each tube. The tube was rinsed 6 times with wash buffer consisting of TBST-0.05%. The wells were filled with 200 μL of TBST-0.5% containing 1 mg/mL BSA and then 10 μL (over 1010 pfu) of purified phage stock was added. The samples were incubated at room temperature for 60 min with slow shaking. The non-binding phage was removed by washing 6 times with TBST-0.5%. Then, 100 μL of horseradish peroxidase/anti-M13 antibody conjugate (Amersham USA, Piscataway, N.J.), diluted 1:500 in the blocking buffer, was added and incubated for 1 h at room temperature. The conjugate solution was removed and was washed 6 times with TBST-0.5%. TMB substrate (200 μL), obtained from Pierce Biotechnology (Rockford, Ill.) was added to each well and the color was allowed to develop for 5 to 30 min, typically for 10 min, at room temperature. Then, stop solution (200 μL of 2 M H2SO4) was added to each well and the solution was transferred to a 96-well plate and the A450 was measured using a microplate spectrophotometer (Molecular Devices, Sunnyvale, Calif.). The resulting absorbance values are given in Tables 3 and 4. “BoEn P” means bovine polished enamel and BoEn means bovine enamel.







TABLE 3







Phage ELISA Results on Polished Enamel-Binding


Assay of Selected Phage Candidates












SEQ ID
O.D.


Phage ID
Amino Acid Sequence
NO:
at 450 nm













Control
no phage
—
0.112


 


BoEn P2
EPPTRTRVNNHTVTVQAQQH
90
0.641


 


BoEn P3
GYCLRGDEPAVCSG
91
0.665


 


BoEn P5
NFCETQLDLSVCTV
93
0.797


 


BoEn P6
NTCQPTKNATPCSA
94
0.83


 


BoEn P8
THNMSHFPPSGHPKRTAT
96
2.02
















TABLE 4







Phage ELISA Results on Tooth Enamel-Binding


Assay of Selected Phage Candidates












SEQ ID
O.D.


Phage ID
Amino Acid Sequence
NO.
at 450 nm













Control
no phage
—
0.193


 


BoEn 1
AANPHTEWDRDAFQLAMPPK
99
1.402


 


BoEn 5
LNDQRKPGPPTMPTHSPAVG
103
0.944


 


BoEn 6
NTCATSPNSYTCSN
104
2.38


 


BoEn 7
SDCTAGLVPPLCAT
105
0.892


 


BoEn 9
VGTMKQHPTTTQPPRVSATN
107
0.568


 


BoEn 10
YSETPNDQKPNPHYKVSGTK
108
3.942









Example 5
Preparation of Cellulose Acetate-Coated Metal Oxide Particles
Approximately 20 g of iron oxide pigment (Unipure Red LC381, Sensient Technologies Corp, Milwaukee, Wis.), 10 g of a 10% solution of cellulose acetate in acetone (Eastman Chemical Company, Kingsport, Tenn.; catalog #CA398-10), and 90 g of acetone were mixed in a blender for 50 minutes. The mixture was then transferred to an Erlenmeyer flask with a magnetic stirrer and 50 mL of petroleum ether was slowly added with stirring. The mixture was filtered and the solid was washed with petroleum ether and dried in a vacuum oven at 60° C., yielding 20.2 g of a red solid with a particle size (average equivalent diameter; d50) of 2.5 microns.
Example 6
Selection of Cellulose Acetate-Binding Peptides Using Standard Biopanning
The purpose of this example was to identify phage peptides that bind cellulose acetate coated particles using standard phage display biopanning method.
Commercial iron oxide particles obtained from Sensient Technologies (Unipure Red LC381) were coated with cellulose acetate as described in Example 5. The cellulose acetate-coated iron oxide particles were incubated in SUPERBLOCK® blocking buffer (Pierce Chemical Company, Rockford, Ill.; Prod. #37535) for 1 hour at room temperature, followed by 3 washes with TBST (TBS in 0.5% TWEEN® 20). Libraries of phage containing random peptide inserts (1011 pfu) from 7 to 20 amino acids were added to each tube. After 60 minutes of incubation at room temperature and shaking at 50 rpm, unbound phages were removed by aspirating the liquid out of each well followed by 6 washes with 1.0 mL TBS containing the detergent TWEEN® 20 (TBST, T-0.5%) and 30% of Neutrogena shampoo (Neutrogena Clean Replenishing, Moisturizing Shampoo, Neutrogena Corp., Los Angeles, Calif. 90045).
The particle samples were then transferred to clean tube, and 200 μL of elution buffer consisting of 1 mg/mL BSA in 0.2 M glycine-HCl, pH 2.2, was added to each well and incubated for 10 min at room temperature to elute the bound phages. Then, 32 μL of neutralization buffer consisting of 1 M Tris-HCl, pH 9.2, was added to each tube. The phage particles, which were in the elution buffer as well as on the particles, were amplified by incubating with diluted E. coli ER2738 cells, from an overnight culture diluted 1:100 in LB medium, at 37° C. for 4.5 h. After this time, the cell culture was centrifuged for 30 sec and the upper 80% of the supernatant was transferred to a fresh tube, 1⁄6 volume of PEG/NaCl (20% polyethylene glyco-800, 2.5 M sodium chloride) was added, and the phage was allowed to precipitate overnight at 4° C. The precipitate was collected by centrifugation at 10,000×g at 4° C. and the resulting pellet was resuspended in 1 mL of TBS. This was the first round of amplified stock. The amplified first round phage stock was then tittered according to the standard protocol. For the 2nd, 3rd and 4th round of biopanning, more than 2×1011 pfu of phage stock from the previous round was used. The biopanning process was repeated under the same conditions as described above.
After the 4th round of biopanning, 95 random single phage plaque lysates were prepared following the manufacture's instructions (New England BioLabs) and the single stranded phage genomic DNA was purified using the QIAprep Spin M13 Kit (Qiagen, Valencia, Calif.) and sequenced at the DuPont Sequencing Facility using −96 gIII sequencing primer (5′-CCCTCATAGTTAGCGTAACG-3′), given as SEQ ID NO: 68. The displayed peptide is located immediately after the signal peptide of gene III. Based on the peptide sequences, 30 phage candidates showed significant enrichment were selected for further pellicle binding analysis. The amino acid sequences of selected phage candidates were listed in Table 5.







TABLE 5







Amino Acid Sequences of Cellulose


Acetate-Binding Candidates











Phage ID
Amino Acid Sequence
SEQ ID NO:







CA-1
ATTPPSGKAAAHSAARQKGN
60



 



CA-2
DTIHPNKMKSPSSPL
61



 



CA-3
NGNNHTDIPNRSSYTGGSFA
62



 



CA-4
SDETGPQIPHRRPTW
63










Example 7
Characterization of Selected Peptides for Cellulose Acetate-Binding Activities
Enzyme-linked immunosorbent assay (ELISA) was used to evaluate the cellulose acetate coated iron oxide particle-binding affinity of the biopanning selected peptide candidates (Example 6; biotinylated peptides phage ID: CA-1 to CA-4). The identified peptides were synthesized using a standard solid-phase synthesis method (see U.S. patent application Ser. No. 11/251/715). All peptides were modified to contain a biotinylated lysine residue at the C-terminus of the amino acid binding sequence for detection purposes (Table 6).
The cellulose acetate-coated iron oxide particles were dispersed in water at 2.5 mg per mL. The dispersion was made by vortex the mix for 1 min, which gave an average particle size of 0.5 μm in diameter. The particle dispersion (1 mL each) was then centrifuged for 2 min at 5000 rpm, the aqueous was removed by aspirating it out of each tube. The tubes were incubated in SUPERBLOCK® blocking buffer (Pierce Chemical Company, Rockford, Ill.; Prod. #37535) for 1 hour at room temperature ( ̃22° C.), followed by 3 washes with TBST (TBS in 0.05% TWEEN® 20). Then tubes were then rinsed 3 times with wash buffer consisting of TBST-0.05% using the same centrifugation and aspiration methods. Peptide binding buffer consisting of 20 μM biotinylated peptides in TBST and 1 mg/mL BSA was added to the particles and incubated for 1 hour at room temperature ( ̃22° C.), followed by 6 TBST washes. Then, the streptavidin-alkaline phosphatase (AP) conjugate (Pierce Chemical Co., Rockford, Ill.) was added to each well at standard concentration and incubated for 1 hour at room temperature, followed by 6 times of washes with TBST. All particles were then transferred to new tubes and then the color development and the absorbance measurements were performed following the manufacturer's standard protocols. The resulting absorbance values, reported as the mean of at least three replicates, and the standard error of the mean (SEM) are given in Table 6.








TABLE 6







Peptide ELISA Results on



Cellulose Acetate-Coated Pigment-Binding


Activities












Amino Acid Sequence
O.D.




Phage ID
(SEQ ID NO.)
at 405 nm
SEM














No peptide
none
0.066
0.003



 


CA-1
ATTPPSGKAAAHSAARQKGN-K-biotin-NH2
0.269
0.017



(SEQ ID NO: 64)


 


CA-2
DTIHPNKMKSPSSPL-K-biotin-NH2
0.207
0.013



(SEQ ID NO: 65)


 


CA-3
NGNNHTDIPNRSSYTGGSFA-K-biotin-NH2
0.279
0.03



(SEQ ID NO: 66)


 


CA-4
SDETGPQIPHRRPTW-K-biotin-NH2
0.141
0



(SEQ ID NO: 67)









Example 8
Biological Production of a Triblock Peptide-Based Reagent
The purpose of this Example was to prepare a triblock peptide-based reagent using recombinant DNA and molecular cloning techniques. The method for recombinantly producing the triblock peptide-based reagent is described in United States Patent Application Publication No. 2007-0065387; herein incorporated by reference.
The triblock peptide-based conjugate was comprised of multiple tooth-binding peptide blocks, peptide spacers, and multiple cellulose acetate-binding peptide blocks. The peptides were expressed in E. coli as inclusion bodies. Additional amino acid sequences (i.e., peptide tags) were fused to the triblock peptide-based reagent sequence in order to promote inclusion body formation. Acid-labile Asp-Pro (DP) sequences were placed between the peptide tag and the triblock peptide-based reagent sequence to facilitate isolation of the triblock peptide-based reagent from the inclusion body peptide tag.
Construction of Production Strains
The sequence of the triblock peptide-based reagent produced is illustrated in Table 7. DNA sequences were designed to encode the peptide-based reagent using favorable codons for E. coli expression and to avoid sequence repeats and mRNA secondary structure. The gene was synthesized by DNA 2.0, Inc. (Menlo Park, Calif.) using proprietary software (Gustafsson et al., Trends in Biotechnol. 22(7):346-355 (2004)). The DNA sequence encoding the amino acid sequence was followed by two termination codons and a recognition site for endonuclease AscI. The GS amino acid sequence at the N-terminus was encoded by a recognition site for endonuclease BamHI (GGA/TCC). The C-terminus was designed to include a His-tag.







TABLE 7







Peptide Sequence of Peptide-Based Reagent









Peptide

SEQ ID


Reagent
Peptide Sequence
NO:





DE020
PHMASMTGGQQMGS
116



(Spacer)-YSETPNDQKPNPHYKVSGTK



(tooth-binding peptide)-



GPEEAAKKEEAAKKPA



(spacer)-



YSETPNDQKPNPHYKVSGTK



(tooth-binding peptide)-



GSGGGGSGSGGGGS



(spacer)-



VRPNLHRKAKAKPDHKQSENRKVPFYSHG



SSG



(cellulose acetate-binding peptide)-



GGG (spacer)-



VRPNLHRKAKAKPDHKQSENRKVPFYSHG



SSG



(cellulose acetate-binding peptide)-



GGHHHHHH



(spacer/His-tag)









The genes were assembled from synthetic oligonucleotides and cloned into a standard plasmid cloning vector by DNA 2.0, Inc. The sequences were then verified by DNA sequencing by DNA 2.0, Inc.
The synthetic gene was excised from the cloning vector with the endonuclease restriction enzymes BamHI and AscI and ligated into an expression vector using standard recombinant DNA methods. The vector pKSI(C4)E-HC77643 (SEQ ID NO: 117) (U.S. provisional patent application No. 60/951,993) was used to express the gene encoding the peptide-based reagent.
The DNA sequence encoding the peptide-based reagent (Table 7) was inserted into pKSI(C4)E-HC77643 by substituting the sequence in the vector between the BamHI and AscI sites. Plasmid DNA containing the peptide encoding sequence and vector DNA was digested with restriction enzymes BamHI and AscI, then the peptide-encoding sequence and vector DNA were mixed and ligated by phage T4 DNA ligase using standard DNA cloning procedures, which are well known to those skilled in the art. The correct construct was identified by restriction analysis and was verified by DNA sequencing.
The coding sequence was operably-linked to the bacteriophage T7 gene 10 promoter and was expressed as a fusion protein, fused with the variant KSI solubility tag.
To test the expression of the peptide-based reagent, the expression plasmid was transformed into the BL21-AI E. coli strain (Invitrogen, catalog no. C6070-03). To produce the recombinant peptide, 50 mL of LB-ampicillin broth (10 g/L bacto-tryptone, 5 g/L bacto-yeast extract, 10 g/L NaCl, 100 mg/L ampicillin, pH 7.0) was inoculated with the transformed bacteria and the culture was shaken at 37° C. until the OD600 reached 0.6. The expression was induced by adding 0.5 mL of 20 wt % L-arabinose to the culture and shaking was continued for another 4 h.
Growth Conditions
The BL21-AI E. coli cells containing the expression plasmid encoding the peptide-based reagent was grown for 20 hours at 37° C. with agitation (200 rpm) in 2.8-L Fernbach flasks containing 1-L of modified ZYP-5052 auto-induction media (Studier, F. William, Protein Expression and Purification (2005) 41 L207-234). The media composition per liter was as follows: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L NaCl, 50 mM Na2HPO4, 50 mM KH2PO4, 25 mM (NH4)2SO4, 3 mM MgSO4, 0.75% glycerol, 0.075% glucose and 0.05% L-arabinose (inducer for BL21 AI T7 system). Under these conditions about 20 g/L wet weight of cells were obtained per liter.
Inclusion Body Isolation
The entire process was performed in one 500-mL bottle. Cells were separated from the growth media by centrifugation and washed with 200-mL (10 g cell paste/100-mL buffer) 20 mM Tris buffer and 10 mM EDTA at pH 8.0. The cell paste was resuspended in 200-mL of 20 mM Tris buffer and 10 mM EDTA at pH 8.0 with added lysozyme (5 mg/200 mL) and taken through at lease one freeze-thaw cycles to facilitate lysis. Lysis was completed by sonication and the inclusion body paste was recovered by centrifugation (9000 RCF 20 minutes at 4° C.). Each additional wash step included resuspension of the inclusion body paste, followed by sonication and centrifugation (9000 RCF 20 minutes at 4° C.). Wash steps included a high pH wash (50 mM Tris HCL pH 9.0) followed by additional washes with 20 mM Tris-HCl pH 8.0. Typically 5 g/L inclusion body paste was recovered.
Acid Cleavage
The recovered inclusion body paste was resuspended in 100 mL of pure water and the pH of the mixture adjusted to 2.2 using HCl. The acidified suspension was heated to 70° C. for 14 hours with agitation to complete cleavage of the DP site separating the fusion peptide from the product peptide.
Oxidative Cross-Linking to Separate the IBT from the Peptide of Interest
The product was cooled to  ̃5° C. then the pH neutralized to 5.3 using NaOH and cooled for an additional 1 hour at  ̃5° C. to facilitate precipitation of cysteine cross-linked KSI (C4)E tag. The mixture was then centrifuged at 10000 RCF for 30 minutes at 4° C. The pellet contained the inclusion body fusion partner KSI (C4)E.
Results after Oxidative Cross-Linking:
SDS-PAGE gel analysis of both the precipitate paste and the remaining soluble fraction showed the presence of KSI(C4)E in the insoluble paste with the desired peptide (the peptide-based reagent) remaining in the soluble fraction.
The supernatant contained the peptide of interest (SEQ NO: 116) as confirmed by HPLC analysis. Further analysis of the isolated peptide by LCMS confirmed the absence of contaminating KSI fragments seen with the KSI (C4) version which contains 5 potential internal acid cleavable “D” sequence and 1 preferred acid cleavage site (Asp-Pro).
Example 9
Prophetic
Production of a Peptide-Based Reagent in a 6-L Fermentation
The purpose of this Example is to describe how to produce a peptide-based reagent in a 6-L batch fermentation.
The recombinant E. coli strain comprising the expression vector encoding at least one of the present peptide-based reagents is grown in a 6-L fermentation, which is run in batch mode initially, and then in fed-batch mode. The composition of the fermentation medium is given in Table 8. The pH of the fermentation medium is maintained as 6.7. The fermentation medium is sterilized by autoclaving, after which the following sterilized components are added: thiamine hydrochloride (4.5 mg/L), glucose (22.1 g/L), trace elements, see Table 9 (10 mL/L), ampicillin (100 mg/L), and inoculum (seed) (125 mL). The pH is adjusted as needed using ammonium hydroxide (20 vol %) or phosphoric acid (20 vol %). The added components are sterilized either by autoclaving or filtration.







TABLE 8







Composition of Fermentation Medium










Component
Concentration















KH2PO4
9
g/L



(NH4)2HPO4
4
g/L



MgSO4•7H2O
1.2
g/L



Citric Acid
1.7
g/L



Yeast extract
5.0
g/L



Mazu DF 204 Antifoam
0.1
mL/L

















TABLE 9







Trace Elements










Component
Concentration, mg/L














EDTA
840



CoCl2•H2O
250



MnCl2•4H2O
1500



CuCl2•2H2O
150



H3BO3
300



Na2MoO4•2H2O
250



Zn(CH3COO)2•H2O
1300



Ferric citrate
10000










The operating conditions that can be used for the fermentations are summarized in Table 10. The initial concentration of glucose is around 22.1 g/L. When the initial residual glucose is depleted, a pre-scheduled, exponential glucose feed is initiated starting the fed-batch phase of the fermentation run. The glucose feed (see Tables 11 and 12) contains 500 g/L of glucose and is supplemented with 5 g/L of yeast extract. The components of the feed medium are sterilized either by autoclaving or filtration. The goal is to sustain a specific growth rate of 0.13 h−1, assuming a yield coefficient (biomass to glucose) of 0.25 g/g, and to maintain the acetic acid levels in the fermentation vessel at very low values (i.e., less than 0.2 g/L). The glucose feed is continued until the end of the run. Induction is initiated with a bolus of 2 g/L of L-arabinose at the selected time (i.e., 15 h of elapsed fermentation time). A bolus to deliver 5 g of yeast extract per liter of fermentation broth is added to the fermentation vessel at the following times: 1 h prior to induction, at induction time, and 1 h after induction time. The fermentation run is terminated after about 20 h of elapsed fermentation time, and about 5 h after the induction time.







TABLE 10







Fermentation Operating Conditions










Condition
Initial
Minimum
Maximum
















Stirring
220
rpm
220
rpm
1200
rpm


Air Flow
3
SLPM
3
SLPM
30
SLPM


Temperature
37°
C.
37°
C.
37°
C.










pH
6.7
6.7
6.7













Pressure
0.500
atm
0.500
atm
0.500
atm



(50.7
kPa)
(50.7
kPa)
(50.7
kPa)










Dissolved O2*
20%
20%
20%





*Cascade stirrer, then air flow.













TABLE 11







Composition of Feed Medium










Component
Concentration















MgSO4•7H2O
2.0
g/L



Glucose
500
g/L



Ampicillin
150
mg/L



(NH4)2HPO4
4
g/L



KH2PO4
9
g/L



Yeast extract
5.0
g/L



Trace Elements - Feed (Table 5)
10
mL/L

















TABLE 12







Trace Elements - Feed










Component
Concentration, mg/L














EDTA
1300



CoCl2•H2O
400



MnCl2•4H2O
2350



CuCl2•2H2O
250



H3BO3
500



Na2MoO4•2H2O
400



Zn(CH3COO)2•H2O
1600



Ferric citrate
4000










Isolation and Purification of Peptide:

After completion of the fermentation run, the entire fermentation broth is passed three times through an APV model 2000 Gaulin type homogenizer at 12,000 psi (82,700 kPa). The broth is cooled to below 5° C. prior to each homogenization. The homogenized broth is immediately processed through a Westfalia WHISPERFUGETM (Westfalia Separator Inc., Northvale, N.J.) stacked disc centrifuge at 700 mL/min and 12,000 RCF to separate inclusion bodies from suspended cell debris and dissolved impurities. The recovered paste is re-suspended at 15 g/L (dry basis) in water and the pH is adjusted to a value between 8.0 and 10.0 using Na2CO3/NaOH buffer. The pH is chosen to help remove cell debris from the inclusion bodies without dissolving the inclusion body proteins. The suspension is passed through the APV 2000 Gaulin type homogenizer at 12,000 psi (82,700 kPa) for a single pass to provide rigorous mixing. The homogenized high pH suspension is immediately processed in a Westfalia WHISPERFUGETM stacked disc centrifuge at 700 mL/min and 12,000 RCF to separate the washed inclusion bodies from suspended cell debris and dissolved impurities. The recovered paste is resuspended at 15 gm/L (dry basis) in pure water. The suspension is passed through the APV 2000 Gaulin type homogenizer at 12,000 psi (82,700 kPa) for a single pass to provide rigorous washing. The homogenized suspension is immediately processed in a Westfalia WHISPERFUGETM stacked disc centrifuge at 700 mL/min and 12,000 RCF to separate the washed inclusion bodies from residual suspended cell debris and NaOH.
The recovered paste is resuspended in pure water at 25 g/L (dry basis) and the pH of the mixture is adjusted to 2.2 using HCl. The acidified suspension is heated to 70° C. for 5 to 14 h to complete cleavage of the DP site separating the fusion peptide from the product peptide without damaging the target peptide. The product slurry is adjusted to pH 5.1 (note: the pH used here may vary depending on the solubility of the peptide being recovered) using NaOH and then is cooled to 5° C. and held for 12 h. The mixture is centrifuged at 9000 RCF for 30 min and the supernatant is decanted. The supernatant is then filtered with a 0.45 μm membrane. For some low solubility peptides, multiple washes of the pellet may be required to increase peptide recovery.
The filtered product is collected and concentrated by vacuum evaporation by a factor of 2:1 before lyophilization. Spectrophotometric detection at 220 and 278 nm is used to monitor and track elution of the product peptide.
Example 10
Preparation of Cellulose Acetate-Coated Whitening Agent
Rutile titanium dioxide (DuPont, Wilmington, Del.) was first coated with silica by a process described in Iler in U.S. Pat. No. 2,885,366 with a 3% silica loading. Approximately 20 g of the silica-coated pigment was combined with 10 g of cellulose acetate (Eastman Chemical Company, Kingsport, Tenn.; catalog #CA398-10) and 90 g acetone in a stainless steel blender and mixed for 50 minutes. The mixture was transferred to an Erlenmeyer flask and stirred with slow addition of 60 mL petroleum ether. The mixture was filtered and the solid was washed with petroleum ether and dried in a vacuum oven at 60° C., yielding 20.4 g of a white solid with a particle size (average equivalent diameter, d50) of 1.2 microns.
Approximately 10 g of the cellulose acetate-coated titanium dioxide was combined with 25 g 0.5 mm glass beads and 40 g water in a SPEEDMIXERTM DAC150 FVZ-K (FlackTek Inc., Landrum, S.C.). The mixture was processed for a total of 20 minutes. Following mixing, the solution was filtered to remove the glass beads. The resulting pigment dispersion was an opaque white solution with a particle size (average equivalent diameter, d50) of 435 nm.
Example 11
Tooth Whitening Using a Polymer-Coated White Colorant in Conjunction with a Peptide-Based Reagent
The purpose of this Example was to demonstrate a process for whitening teeth using a polymer-coated white colorant in conjunction with a peptide-based reagent and to compare the results to the whitening obtained with the polymer-coated white colorant alone. The peptide-based reagent comprising multiple tooth-binding peptides, spacers, and cellulose acetate-binding peptide sequences was prepared as described in Example 8.
The cellulose acetate-coated titanium dioxide was prepared as described in Example 10. The peptide-based reagent described in Example 8 (SEQ ID NO:116) was used. The peptide-based reagent was diluted with 25 mM Tris-HCl buffer including 150 mM NaCl at pH 7.5 to give a final concentration of 5 μM. The cellulose acetate-coated titanium dioxide dispersion described in Example 10 was further diluted from 20% solids to 0.25% solids in 25 mM Tris-HCl buffer at pH 7.5.
Tooth Substrates:
Bovine Teeth (SE Dental, Baton Rouge, La.) were used as model substrate to test with the engineered peptide-based reagent designed to bind to tooth enamel (SEQ ID NO:116). The teeth were prepared by cleaning in detergent and then disinfecting in 70% Ethanol prior to use. Each clean tooth was measured for color using an X-RITE® SP64 Spectrometer to collect a reflectance spectrum and CIELAB color coordinates, L*, a* and b*. A staining solution was made with 100 mL of boiling water exposed to 1 Lipton tea bag for 5 minutes. After the solution cooled to room temperature, each tooth was allowed to incubate in the staining solution for approximately 3 days. The teeth were removed and rinsed with water and dried. Color measurement was obtained for each tooth.
Tooth Whitening
The stained bovine enamel substrates were exposed to either the 5 μM solution of peptide or as a control, the buffer used to make the peptide solution. This exposure was for 30 minutes with gentle agitation in a vortex mixer. Following peptide binding, the teeth were removed and rinsed in buffer and placed in a 0.25% solution of cellulose acetate coated titanium dioxide for 30 minutes. The teeth were removed, rinsed in buffer, blotted dry and measured for color using the X-RITE® spectrometer. The teeth were placed back in the same pigment solutions for an additional 2.5 hr and removed, rinsed and measured again. Results are reported in Table 13.
Measurement of Color Intensity
The color intensity at each step mentioned above (e.g., to determine the change in tooth whitening) was measured using a X-RITE® SP64TM Sphere Spectrophotometer (X-Rite, Inc., Grandville, Mich.), by placing the front of the bovine tooth (treated or control) sample into a sample holder and calculating L*, a* and b* parameters representing the photometer response using a standard D65 illuminant. An initial baseline values of L*, a* and b* were measured prior to peptide treatment for the tea-stained bovine samples. All measurements are the average of three individual determinations. Delta E values are calculated using equation 1 below:

Delta E=((L*1−L*2)2+(a1−a2)2+(b1−b2)2)1/2  (1)

where L*=the lightness variable and a* and b* are the chromaticity coordinates of CIELAB colorspace as defined by the International Commission of Illumination (CIE) (Minolta, Precise Color Communication—Color Control From Feeling to Instrumentation, Minolta Camera Co., 1996). Larger Delta E value is indicative of an increase in whiteness. In one embodiment, a larger Delta E for the treatment group (peptide-based reagent+polymer coated white colorant) versus the Delta E of the peptide-free control is used to determine the relative increase in whiteness.

In another aspect, the measurement of the whiteness of teeth may also be reported as a whiteness index wherein an increase in the whiteness after treatment with the present compositions is indicative of increased whitening. The whiteness index (WI) is defined by the International Commission on Illumination (CIE) and described in ASTM method E313-05 and calculated for D65/10 incident light as:

WI=Y+800*(0.3138−x)+1700*(0.3310−y)

where Y, x, and y are the luminance factor and the chromaticity coordinates respectively of the enamel substrate.








TABLE 13







Whitening of Bovine Enamel using Engineered


Peptide and Cellulose Acetate-coated TiO2.










Control
Treated











Tea stain 3 days











L*
71.6364
67.0271



a*
2.93059
3.96396



b*
20.7223
22.7952



ΔE
0.00
0.00







Peptide/buffer (30 min) + CA-TiO2 (30 min)











L*
73.336
71.1068



a*
1.69143
1.63501



b*
18.2412
18.0418



ΔE
3.25
6.68







CA-TiO2 (2.5 hr)











L*
73.7424
73.9558



a*
1.62671
1.49585



b*
17.8343
18.0851



ΔE
3.80
8.73










Example 12
Selection of Tooth (Pellicle)-Binding Peptides Using Biopanning
The purpose of this Example was to identify phage peptides that bind to tooth pellicle formed in vivo on bovine enamel.
Bovine enamel incisors were obtained from SE Dental (Baton Rouge, La.). The teeth were cut to approximately 5 mm squares and polished to remove surface debris. Enamel blocks were sterilized and sewn into intra-oral retainers in order to expose the enamel surface to the human oral environment. A retainer with 2 to 4 enamel blocks was worn in the human mouth for 30 min to form a pellicle layer on the enamel. After incubation, the enamel blocks were removed from the retainer, rinsed with water and embedded in a well plate contained molding material so as to only expose the pellicle-coated enamel surface in the well. The plate was sterilized with UV light for 10 minutes.
The substrates were then incubated in blocking buffer for 1 hour at room temperature (1 mg/mL bovine serum albumin in phosphate buffered saline pH 7.2 (Pierce BUPHTM #28372) with 0.1% TWEEN® 20 (PBST), followed by 2 washes with PBST. Libraries of phage containing random peptide inserts (10′′ pfu) from 15 to 20 amino acids were added to each well. After 30 minutes of incubation at 37° C. and shaking at 50 rpm, unbound phage were removed by aspirating the liquid out of each well followed by 6 washes with 1.0 mL PBST.
The enamel blocks were then transferred to clean tubes and 1 mL of elution buffer consisting of 1 mg/mL BSA in 0.2 M glycine-HCl, pH 2.2, was added to each well and incubated for 10 min at room temperature to elute the bound phages. Then, 167 μL of neutralization buffer consisting of 1 M Tris-HCl, pH 9.1, was added to each well. The phage particles, which were in the elution buffer as well as on the enamel blocks, were amplified by incubating with 20 mL diluted E. coli ER2738 cells, from an overnight culture diluted 1:100 in LB medium, at 37° C. for 4.5 h. After this time, the cell culture was centrifuged for 2 min and the upper 15 mL of the supernatant was transferred to a fresh tube, 2.5 mL of PEG/NaCl (20% polyethylene glycol-800, 2.5 M sodium chloride) was added, and the phage were allowed to precipitate overnight at 4° C. The precipitate was collected by centrifugation at 10,000×g at 4° C. and the resulting pellet was resuspended in 1 mL of PBS. This was the first round of amplified stock. The amplified first round phage stock was then tittered according to the standard protocol. For subsequent rounds of biopanning, more than 2×1011 pfu of phage stock from the previous round was used. Each additional round after the first also included additional washes with 0.5% sodium lauryl sulfate in water (Spectrum), two washes with carbonate buffer pH 9.4 (Pierce BUPHTM Carbonate-Bicarbonate Buffer #28382) and 2 washes with 50 mM phosphate buffer, pH 2.5.
The biopanning process was repeated an additional 3 more rounds under the same conditions as described above with an additional exposure of the phage stock to oral soft tissue. The phage stock amplified from the 2rd round was exposed first to EPIORALTM and EPIGINGIVALTM soft tissues (MatTek Corp, Ashland, Mass.) by incubating 8 μL of the 2nd round phage stock+42 μL of blocking buffer (PBST+1 mg/mL BSA) for 60 min. The solution was removed from the tissue and an additional 50 μL of PBS was incubated with the tissue for 30 min. The solutions were combined and used in additional rounds of biopanning as described above.
After the 3rd round of biopanning and each subsequent round, 95 random single phage plaques were isolated and the single stranded phage genomic DNA was prepared using the Illustra TempliPhi 500 Amplification Kit (GE Healthcare, Piscataway, N.J.) and sequenced at the DuPont Sequencing Facility using −96 gIII sequencing primer (5′-CCCTCATAGTTAGCGTAACG-3′; SEQ ID NO: 68). The displayed peptide was located immediately after the signal peptide of gene III. Based on the peptide sequences, 31 phage candidates were identified for further pellicle binding analysis (Table 14).







TABLE 14







Tooth-binding Peptides Identified from


Biopanning on 30 min in vivo Pellicle











Peptide





ID
Amino Acid Sequence
SEQ ID NO







DenP 01
NGNNHTDIPNRSSYTGGSFA
119



 



DenP 02
TMTNHVYNSYTEKHSSTHRS
120



 



DenP 03
TTYHYKNIYQESYQQRNPAV
121



 



DenP 04
VEPATKNMREARSSTQMRRI
122



 



DenP 05
YLLPKDQTTAPQVTPIVQHK
123



 



DenP 06
ASNLDSTFTAINTPACCT
124



 



DenP 07
EFPYYNDNPPNPERHTLR
125



 



DenP 08
GMPTRYYHNTPPHLTPKF
126



 



DenP 09
HKNAIQPVNDATTLDTTM
127



 



DenP 10
AVVPADLNDHANHLS
128



 



DenP 11
DLGTFPNRTLKMAAH
129



 



DenP 12
FDGIGLGTATRHQNR
130



 



DenP 13
QAAQVHMMQHSRPTT
131



 



DenP 14
SEARARTFNDHTTPMPII
132



 



DenP 15
ELDHDSRHYMNGLQRKVT
133



 



DenP 16
GPQHVLMQDTHQGYAFDN
134



 



DenP 17
TTGSSSQADTSASMSIVPAH
135



 



DenP 18
KAPIANMLQPHSYQYSVA
136



 



DenP 19
TYQGVPSWPAVIDDAIRR
137



 



DenP 20
VNPNWVETQALHQPPGNT
138



 



DenP 21
DHNNRQHAVEVRENKTHTAR
139



 



DenP 22
IYPNESMSTSNVRGPYHP
140



 



DenP 23
HDPNHLTHQARTIYRNANHT
141



 



DenP 24
SNATMYNIQSHSHHQ
142



 



DenP 25
ANELSTYAQTNPGSG
143



 



DenP 26
DTIHPNKMKSPSSPL
144



 



DenP 28
APPTYQTASYPHNLPSKRKM
145



 



DenP 29
QVPDYLSPTHQKKAFLEIPT
146



 



DenP 30
TNDLHANPFTGTYIAPDPTS
147



 



DenP 32
HKNENIMQYNVNDRWHITPA
148



 



DenP 33
IDGPHHSPVHRYHTPSIT
149










Example 13
Characterization of Tooth (Pellicle)-Binding Candidates on Pellicle Surface
A total of 29 selected phage candidates from Table 14 were used in phage ELISA Experiment to determine binding affinity and coverage of each phage on pellicle substrates. Purified phage lysates were used for binding to pellicle coated bovine enamel using an anti-M13 phage antibody conjugated to horseradish-peroxidase, followed by the addition of chromogenic agent TMB, obtained from Pierce Biotechnology (Rockford, Ill.). The plates were read at A450nm.
Enamel substrates were cut to approximately 7 mm squares and mounted on wax mounting for incubation in the mouth for 30 min to form a pellicle-coated surface. The pellicle-coated enamel substrates were removed from the wax backing and placed in well plates with the pellicle surface exposed as in Example 12. Each pellicle-coated substrate was incubated for 1.5 h at room temperature with 1 mL of blocking buffer, consisting of 1 mg/mL BSA in PBST (Pierce BUPHTM #28372 with 0.1% TWEEN®20). The blocking buffer was removed by aspirating the liquid out of each well. The tube was rinsed 2 times with wash buffer consisting of PBST. The wells were filled with 1 mL of 1011 pfu purified phage stock which was prepared by diluting in blocking buffer. The samples were incubated for 30 min with slow shaking at 37° C. The non-binding phage was removed by washing 5 times with PBST. Then, 500 μL of horseradish peroxidase/anti-M13 antibody conjugate (Amersham USA, Piscataway, N.J.), diluted 1:500 in the blocking buffer, was added and incubated for 1 h at room temperature ( ̃22° C.). The conjugate solution was removed and was washed 3 times with PBST. Each enamel substrate was removed from the well and washed again in a 15-mL test tube with 5 mL of PBST. Each enamel substrate was then mounted in a clean well plate with only the enamel surface exposed. A 1:1 solution of TMB substrate and H2O2 (200 μL), obtained from Pierce Biotechnology (Rockford, Ill.) was added to each well and the color was allowed to develop for between 5 to 30 min, typically for 10 min, at room temperature (approximately 22° C.). Then, stop solution (100 μL of 2 M H2SO4) was added to each well and the solution was transferred to a 96-well plate and the A450 was measured using a microplate spectrophotometer (Molecular Devices, Sunnyvale, Calif.). The resulting absorbance values, are given in Table 15. The analysis of all 30 pellicle-binding candidates was completed over the course of two days and the results were normalized to an internal control.







TABLE 15







Phage ELISA Results for Pellicle-binding


Peptide Candidates Obtained from Biopanning










Peptide

SEQ ID
O.D. at 450 nm


ID
Amino Acid Sequences
NO:
(normalized)





Control
IPWWNIRAPLNAGGG
150
1.000


 


DenP 01
NGNNHTDIPNRSSYTGGSFA
119
1.002


 


DenP 02
TMTNHVYNSYTEKHSSTHRS
120
1.951


 


DenP 03
TTYHYKNIYQESYQQRNPAV
121
2.495


 


DenP 04
VEPATKNMREARSSTQMRRI
122
1.421


 


DenP 05
YLLPKDQTTAPQVTPIVQHK
123
1.087


 


DenP 07
EFPYYNDNPPNPERHTLR
125
1.500


 


DenP 08
GMPTRYYHNTPPHLTPKF
126
1.182


 


DenP 09
HKNAIQPVNDATTLDTTM
127
1.364


 


DenP 10
AVVPADLNDHANHLS
128
1.619


 


DenP 11
DLGTFPNRTLKMAAH
129
1.663


 


DenP 12
FDGIGLGTATRHQNR
130
2.079


 


DenP 13
QAAQVHMMQHSRPTT
131
0.845


 


DenP 14
SEARARTFNDHTTPMPII
132
2.498


 


DenP 15
ELDHDSRHYMNGLQRKVT
133
1.112


 


DenP 16
GPQHVLMQDTHQGYAFDN
134
2.190


 


DenP 17
TTGSSSQADTSASMSIVPAH
135
0.971


 


DenP 18
KAPIANMLQPHSYQYSVA
136
1.143


 


DenP 19
TYQGVPSWPAVIDDAIRR
137
1.052


 


DenP 20
VNPNWVETQALHQPPGNT
138
1.298


 


DenP 21
DHNNRQHAVEVRENKTHTAR
139
0.728


 


DenP 22
IYPNESMSTSNVRGPYHP
140
1.420


 


DenP 23
HDPNHLTHQARTIYRNANHT
141
1.236


 


DenP 24
SNATMYNIQSHSHHQ
142
0.979


 


DenP 25
ANELSTYAQTNPGSG
143
0.909


 


DenP 26
DTIHPNKMKSPSSPL
144
1.039


 


DenP 28
APPTYQTASYPHNLPSKRKM
145
1.203


 


DenP 29
QVPDYLSPTHQKKAFLEIPT
146
0.976


 


DenP 30
TNDLHANPFTGTYIAPDPTS
147
1.082


 


DenP 32
HKNENIMQYNVNDRWHITPA
148
1.441









Example 14
Characterization of Tooth Pellicle-Binding Candidates on Pellicle Surface
The purpose of this example was to confirm the binding of peptide compositions on pellicle surfaces using synthetically produced peptides.
A total of 20 synthetic peptides were manufactured using sequences obtained from Table 14. Peptides were obtained from Synbiosci (Livermore, Calif.) and included an additional SSRP sequence (SEQ ID NO: 151) at the N-terminus and biotin labeled lysine at the C-terminus.
Enamel substrates were cut to approx. 7 mm squares and mounted on wax mounting for incubation in the mouth for 30 min to form a pellicle coated surface. The pellicle-coated enamel substrates were removed from the wax backing and placed in well plates with the pellicle surface exposed as in Example 1. Each pellicle-coated substrate was incubated for 1 h at room temperature ( ̃22° C.) with 1 mL of blocking buffer, consisting of 1 mg/mL BSA in PBST (Pierce BupHTM #28372 with 0.1% TWEEN® 20). The blocking buffer was removed by aspirating the liquid out of each well. The tube was rinsed 2 times with wash buffer consisting of PBST. The wells were filled with 500 μL of 20 μM peptide solution which was prepared by diluting in blocking buffer. The samples were incubated for 30 min with slow shaking at 37° C. The non-binding peptide was removed by washing 6 times with PBST. Then, 500 μL of horseradish peroxidase/streptavidin conjugate (Pierce #22127), diluted 1:2000 in PBST, was added and incubated for 1 h at room temperature. The conjugate solution was removed and was washed 4 times with PBST.
Each enamel substrate was removed from the well and washed again in a 15-mL test tube with 10 mL of PBST. Each enamel substrate was then mounted in a clean well plate with only the enamel surface exposed. A 1:1 solution of TMB substrate and H2O2 (200 μL), obtained from Pierce Biotechnology (Rockford, Ill.) was added to each well and the color was allowed to develop for between 10 to 20 min, typically for 15 min, at room temperature ( ̃22° C.). Then, 100 μL of solution from each well was transferred to a 96-well reading plate containing stop solution (100 μL of 2 M H2SO4) in each well. The A450 was measured using a microplate spectrophotometer (Molecular Devices, Sunnyvale, Calif.). The resulting absorbance values,) are given in Table 16. The analysis of 20 pellicle-binding candidates was completed over the course of two days and the results were normalized to the best binding candidate from day 1 (DenP03). Each sequence was tested with three replicate enamel coated pellicle substrates.








TABLE 16







Synthetic Peptide ELISA Results for Pellicle-binding



Candidates Obtained from Biopanning.











Pellicle


SEQ



binding

O.D. at 450 nm
ID


peptide ID
Amino Acid Sequence
(normalized)
NO














No
—
−0.001
—



peptide


 


DenP1-A
SSRPNGNNHTDIPNRSSYTGGSFAK(biotin)
0.154
152


 


DenP2-A
SSRPTMTNHVYNSYTEKHSSTHRSK(biotin)
0.273
153


 


DenP3-A
SSRPTTYHYKNIYQESYQQRNPAVK(biotin)
1.000
154


 


DenP4-A
SSRPVEPATKNMREARSSTQMRRIK(biotin)
0.803
155


 


DenP5-A
SSRPYLLPKDQTTAPQVTPIVQHKK(biotin)
0.462
156


 


DenP7-A
SSRPEFPYYNDNPPNPERHTLRK(biotin)
0.356
157


 


DenP11-A
SSRPDLGTFPNRTLKMAAHK(biotin)
0.454
158


 


DenP12-A
SSRPFDGIGLGTATRHQNRK(biotin)
0.475
159


 


DenP13-A
SSRPQAAQVHMMQHSRPTTK(biotin)
0.699
160


 


DenP14-A
SSRPSEARARTFNDHTTPMPIIK(biotin)
0.269
161


 


DenP15-A
SSRPELDHDSRHYMNGLQRKVTK(biotin)
0.460
162


 


DenP16-A
SSRPGPQHVLMQDTHQGYAFDNK(biotin)
0.309
163


 


DenP17-A
SSRPTTGSSSQADTSASMSIVPAHK(biotin)
0.143
164


 


DenP19-A
SSRPTYQGVPSWPAVIDDAIRRK(biotin)
0.712
165


 


DenP20-A
SSRPVNPNWVETQALHQPPGNTK(biotin)
0.590
166


 


DenP22-A
SSRPIYPNESMSTSNVRGPYHPK(biotin)
0.354
167


 


DenP23-A
SSRPHDPNHLTHQARTIYRNANHTK(biotin)
0.850
168


 


DenP28-A
SSRPAPPTYQTASYPHNLPSKRKMK(biotin)
0.811
169


 


DenP29-A
SSRPQVPDYLSPTHQKKAFLEIPTK(biotin)
0.468
170


 


DenP32-A
SSRPHKNENIMQYNVNDRWHITPAK(biotin)
1.135
171









Example 15
Determination of the Peptide Binding Affinity on Pellicle Surface
The purpose of this Example was to determine the affinity and specificity of the pellicle-binding peptides and peptide compositions comprising the pellicle-binding peptides identified in Example 14, measured as MB50 values, using an ELISA assay.
Pellicle-binding peptides, DenP3-A and DenP32-A as described in Table 16, were synthesized using standard solid phage synthesis method and were biotinylated at the C-terminus lysine residue of binding sequence for detection purposes.
Enamel substrates were cut to approx. 4 mm squares and mounted on wax mounting for incubation in the mouth for 30 min to form a pellicle coated surface. The pellicle-coated enamel substrates were removed from the wax backing and placed in well plates with the pellicle surface exposed as in Example 1. Each pellicle-coated substrate was incubated for 1 h at room temperature (2° C.) with 1 mL of blocking buffer, consisting of 1 mg/mL BSA in PBST (Pierce BupHTM #28372 with 0.1% TWEEN® 20). The blocking buffer was removed by aspirating the liquid out of each well. The tube was rinsed 2 times with wash buffer consisting of PBST. The wells were filled with 500 μL of peptide solution which was prepared by diluting in blocking buffer across a range of concentrations. The samples were incubated for 2 h with slow shaking at 37° C. The non-binding peptide was removed by washing 6 times with PBST. Then, 500 μL of alkaline phosphatase/streptavidin conjugate (Pierce), diluted 1:2500 in PBST, was added and incubated for 1 h at room temperature. The conjugate solution was removed and was washed 4 times with PBST.
Each enamel substrate was removed from the well and washed again in a 15-mL test tube with 10 mL of PBST. Each enamel substrate was then mounted in a clean well plate with only the enamel surface exposed. 150 μL of Methyl umbelliferone 4-phosphate (4-MUP) substrate (Sigma) was added to each well and incubated at room temperature for 30 min protected from ambient light. Then, 100 uL of solution from each well was transferred to a 96-well reading plate. Fluorescence was read using a microplate spectrophotometer (Molecular Devices, Sunnyvale, Calif.). The results were plotted as relative fluorescence units versus the concentration of peptide using GraphPad Prism 4.0 (GraphPad Software, Inc., San Diego, Calif.). The MB50 values were calculated from Scatchard plots and are shown Table 17.








TABLE 17







Summar of MB50 Values for Pellicle-Binding



Peptides Against Pellicle Surface











Peptide
SEQ ID





ID
NO
Sequence
MB50 (M)





DenP3-A
154
SSRPTTYHYKNIYQESYQQRNPAVK(biotin)
2.8 × 10−5



 


DenP32-A
171
SSRPHKNENIMQYNVNDRWHITPAK(biotin)
2.5 × 10−5









Example 16
Selection of Additional Pellicle-Binding Peptides Using Standard Biopanning
The purpose of this Example was to identify phage peptides that bind tooth pellicle created with long term exposure in the mouth using standard phage display biopanning.
Bovine enamel incisors were obtained from SE Dental (Baton Rouge, La.). The teeth were cut to approx. 7 mm squares and polished to remove surface debris. Enamel blocks were sterilized and sewn into intra-oral retainers in order to expose the enamel surface to the human oral environment. A retainer with 4 enamel blocks was worn in a human mouth for approximately 8 hours. The retainer was removed from the subject and each enamel block was manually brushed with toothpaste and a soft bristle brush under water. The retainer was reinserted into the subject's mouth for an additional 1 min. The enamel blocks were removed from the retainer, rinsed with water and embedded in a well plate contained molding material so as to only expose the pellicle coated enamel surface in the well. The plate was sterilized with UV light for 10 minutes.
The substrates were then incubated in blocking buffer for 1 hour at room temperature (1 mg/mL bovine serum albumin in phosphate buffered saline pH 7.2 (Pierce BupHTM #28372) with 0.1% TWEEN® 20 (PBST)), followed by 2 washes with PBST (PBS in 0.1% TWEEN® 20). Libraries of phage containing random peptide inserts (1011 pfu) from 16 to 20 amino acids were added to each well. After 30 minutes of incubation at 37° C. and shaking at 50 rpm, unbound phage were removed by aspirating the liquid out of each well followed by 2 washes with 1.0 mL PBST.
The enamel blocks were then transferred to a clean tube and 1 mL of elution buffer consisting of 1 mg/mL BSA in 0.2 M glycine-HCl, pH 2.2, was added to each well and incubated for 10 min to elute the bound phages. Then, 167 μL of neutralization buffer consisting of 1 M Tris-HCl, pH 9.1, was added to each well. The phage particles, which were in the elution buffer as well as on the enamel blocks, were amplified by incubating with 20 mL diluted E. coli ER2738 cells, from an overnight culture diluted 1:100 in LB medium, at 37° C. for 4.5 h. After this time, the cell culture was centrifuged for 2 min and the upper 15 mL of the supernatant was transferred to a fresh tube, 2.5 mL of PEG/NaCl (20% polyethylene glyco-800, 2.5 M sodium chloride) was added, and the phage was allowed to precipitate overnight at 4° C. The precipitate was collected by centrifugation at 10,000×g at 4° C. and the resulting pellet was resuspended in 1 mL of PBS. This was the first round of amplified stock. The amplified first round phage stock was then titered according to the standard protocol. For the 2nd, 3rd 4th and 5th rounds of biopanning, more than 2×1011 pfu of phage stock from the previous round was used. In these subsequent rounds, additional washes processes were included after the initial incubation of the phage. These washes included a 0.5% sodium lauryl sulfate in water (Spectrum), two washes with carbonate buffer pH 9.4 (Pierce BupHTM Carbonate-Bicarbonate Buffer #28382) and 2 washes with 50 mM phosphate buffer, pH 2.5 followed by 5 washes with PBST.
After the 3rd round of biopanning and each subsequent round, 95 random single phage plaques were isolated and the single stranded phage genomic DNA was prepared using the Illustra Templiphi 500 Amplification Kit (GE Healthcare, Piscataway, N.J.) and sequenced at the DuPont Sequencing Facility using −96 gIII sequencing primer (5′-CCCTCATAGTTAGCGTAACG-3′; SEQ ID NO: 68). The displayed peptide was located immediately after the signal peptide of gene III. Based on the peptide sequences, 23 phage candidates were selected for further pellicle binding analysis. These candidates included 3 sequences previously discovered in panning in Example 12.







TABLE 18







Binding Sequences Identified from Biopanning on


Brushed, 8-hour in vivo Pellicle.











ID
Amino Acid Sequence
SEQ ID NO







DenP101
AIEYQHSATTPWTMRTRLPP
172



 



DenP102
EFYPFAEVPPEKSGIGRQVF
173



 



DenP103
GVHQYSRPTVPSYLWTSGQH
174



 



DenP104
GYQPHYVDHTIGWQPMIRPN
175



 



DenP105
QFNQTSHSFMHGTSGYVPGK
176



 



DenP106
SFSWHRGDWELGHQSKTMGM
177



 



DenP107
SMWHDITKRYRNPSEMVSAY
178



 



DenP108
THGNKHQSWTYPSEINHKNY
179



 



DenP109
WHEPHQFSGENTDYSSSMGT
180



 



DenP110
THGNKHQSWTYPSEINHKNY
181



 



DenP111
DGYKLQTSLDWQMWNP
182



 



DenP112
FPSKWYNHHRHITGHV
183



 



DenP113
GGMGALESYRQWNHLA
184



 



DenP114
GINKGQRPPWESWHEN
185



 



DenP115
GYGQYVSQQTWAHSNK
186



 



DenP116
HDHLSWWGQFDRQNLL
187



 



DenP117
MPGHQESIKVQNWNRV
188



 



DenP118
NLHSPWPSHAAHHWST
189



 



DenP119
NQQMKLVPQHWHRAQP
190



 



DenP120
SEKWFNPGPWPKLATQ
191



 



DenP11
DLGTFPNRTLKMAAH
129



 



DenP07
EFPYYNDNPPNPERHTLR
125



 



DenP08
GMPTRYYHNTPPHLTPKF
126










Example 17
Characterization of Tooth Pellicle-Binding Candidates on Pellicle Surface
A total of 18 selected phage candidates were used in a phage ELISA Experiment. Purified phage lysates were used for binding to pellicle-coated bovine enamel using an anti-M13 phage antibody conjugated to horseradish-peroxidase, followed by the addition of chromogenic agent TMB, obtained from Pierce Biotechnology (Rockford, Ill.). The plates were read at A450nm.
Enamel substrates were cut to approx. 4 mm squares, cleaned, sterilized and mounted on wax mounting for incubation in the mouth for 30 min to form a pellicle coated surface. The pellicle coated enamel substrates were removed from the wax backing and placed in well plates with the pellicle surface exposed as in Example 1. Each pellicle-coated substrate was incubated for 1 h at room temperature with 0.5 mL of blocking buffer, consisting of 1 mg/mL BSA in PBST pH 7.2 (Pierce BupHTM #28372 with 0.1% TWEEN® 20). The blocking buffer was removed by aspirating the liquid out of each well. The wells were rinsed 2 times with wash buffer consisting of PBST. The wells were filled with 1 mL of 10′′ pfu purified phage stock which was prepared by diluting in blocking buffer. The samples were incubated for 30 min with slow shaking at 37° C. The non-binding phage was removed by washing 5 times with PBST. Then, 500 μL of horseradish peroxidase/anti-M13 antibody conjugate (Amersham USA, Piscataway, N.J.), diluted 1:500 in the blocking buffer, was added and incubated for 45 min at room temperature. The conjugate solution was removed and was washed 5 times with PBST. Each enamel substrate was removed from the well and washed again in a 15-mL test tube with 10 mL of PBST. Each enamel substrate was then mounted in a clean well plate with only the enamel surface exposed. A 1:1 solution of TMB substrate and H2O2 (200 μL), obtained from Pierce Biotechnology (Rockford, Ill.) was added to each well and the color was allowed to develop for between 5 to 30 min, typically for 10 min, at room temperature. Then, stop solution (100 μL of 2 M H2SO4) was added to each well and the solution was transferred to a 96-well plate and the A450 was measured using a microplate spectrophotometer (Molecular Devices, Sunnyvale, Calif.). The resulting absorbance values, are given in Table 8. The analysis of all 18 pellicle binding candidates was completed over the course of two days and the results were normalized to the result of DenP3 which was measured on both days.







TABLE 19







Phage ELISA Results for Pellicle-binding


Peptide Candidates obtained from


Biopanning on Brushed 8 hr Pellicle,


Screened with 30 min in vivo Pellicle












SEQ ID
O.D. at 450 nm


ID
Amino Acid Sequences
NO
(normalized)





Control
No Phage
—
0.094


 


DenP3
TTYHYKNIYQESYQQRNPAV
121
1.000


 


DenP101
AIEYQHSATTPWTMRTRLPP
172
0.467


 


DenP102
EFYPFAEVPPEKSGIGRQVF
173
0.520


 


DenP103
GVHQYSRPTVPSYLWTSGQH
174
0.879


 


DenP104
GYQPHYVDHTIGWQPMIRPN
175
0.790


 


DenP105
QFNQTSHSFMHGTSGYVPGK
176
0.470


 


DenP106
SFSWHRGDWELGHQSKTMGM
177
1.524


 


DenP107
SMWHDITKRYRNPSEMVSAY
178
0.726


 


DenP108
THGNKHQSWTYPSEINHKNY
179
1.149


 


DenP109
WHEPHQFSGENTDYSSSMGT
180
0.716


 


DenP111
DGYKLQTSLDWQMWNP
182
1.051


 


DenP112
FPSKWYNHHRHITGHV
183
0.413


 


DenP113
GGMGALESYRQWNHLA
184
1.348


 


DenP114
GINKGQRPPWESWHEN
185
0.703


 


DenP115
GYGQYVSQQTWAHSNK
186
0.501


 


DenP116
HDHLSWWGQFDRQNLL
187
1.055


 


DenP117
MPGHQESIKVQNWNRV
188
0.433


 


DenP118
NLHSPWPSHAAHHWST
189
0.641


 


DenP119
NQQMKLVPQHWHRAQP
190
1.051

## Claims

1. A peptide-based reagent having the general structure:


[[(TBP)y-Sq]m-[(PBP)z-Sr]n]p;

wherein
i) TBP is a tooth-binding peptide comprising amino acid SEQ ID NO: 121;
ii) PBP is a polymer-binding peptide comprising amino acid SEQ ID NO: 118;
iii) S is a peptide spacer comprising 1 to about 50 amino acids;
iv) m, n, y and z independently range from 1 to about 10;
v) p is from 1 to 5; and
vi) q an r are each independently 0 or 1, provided that both q and r may not be 0.

2. The peptide-based reagent of claim 1 wherein the peptide spacer comprises amino acids selected from the group consisting of proline, lysine, glycine, alanine, serine, threonine, and combinations thereof.

3. A method for whitening teeth comprising:
(a) providing at least one polymer-coated white colorant;
(b) providing a composition comprising the peptide-based reagent according to claim 1; and
(c) contacting a tooth surface with the at least one polymer-coated white colorant of (a) and the composition of (b) whereby said tooth surface is whitened.

4. The method according to claim 3 further comprising the step (d): applying a composition comprising a polymeric sealant; wherein the polymeric sealant is selected from the group consisting of poly(allylamine), acrylates, acrylate copolymers, polyurethanes, carbomers, methicones, amodimethicones, polyethylenene glycol, beeswax, siloxanes, copolymers of methyl vinyl ether and maleic anhydride, linear and cross linked polyvinylpyrrolidone homopolymers, and combinations thereof.

5. An oral care composition comprising the peptide based-reagent of claim 1 and at least one polymer-coated white colorant.

6. A tooth-binding peptide comprising amino acid sequence SEQ ID NO: 121.

7. A peptide-based reagent comprising the tooth-binding peptide of claim 6.

8. An oral care composition comprising the tooth-binding peptide of claim 6 or the peptide-based reagent of claim 7.

9. The oral care composition of claim 8 further comprising at least one polymer coated white colorant.