U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Hard Winter Wheat Genetics Research: Manhattan, KS
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Central Small Grain Genotyping Laboratory
                    Units
                        Center for Grain and Animal Health Research: Manhattan, KS
                                    Arthropod-Borne Animal Diseases Research Unit
                                    Grain Quality and Structure Research Unit
                                    Stored Product Insect & Engineering Research UnitSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Plains Area Manhattan, Kansas Center for Grain and Animal Health Research Hard Winter Wheat Genetics Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications
- Hessian Fly Research Lab
- Sequencing Facility
- USDA Central Small Grain Genotyping Lab

Title:   Author  item
                                            AGGARWAL, RAJAT - PURDUE UNIVERSITYitem
                                            BENATTI, THIAGO - PURDUE UNIVERSITYitem
                                            GILL, NAVDEEP - PURDUE UNIVERSITYitem
                                            Chen, Ming-Shunitem
                                            Schemerhorn, Brandiitem
                                            Fellers, Johnitem
                                            STUART, JEFF - PURDUE UNIVERSITY
  Submitted to: BMC Genomics
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 6/23/2009
                                    Publication  Date: 7/5/2009
                                    Citation: Aggarwal, R., Benatti, T., Gill, N., Chen, M., Schemerhorn, B.J., Fellers, J.P., Stuart, J.J. 2009. A BAC-based physical map of the Hessian fly (Mayetiola destructor) genome anchored to polytene chromosomes. Biomed Central (BMC) Genomics.  10:293.Interpretive Summary: The Hessian fly (Mayetiola destructor) is one of the most destructive insects of wheat world-wide. Resistant wheat is the best strategy to control the damage caused by this pest.  The challenge for the use of resistant wheat is the ability of the insect to develop new populations that can overcome resistance once a resistant wheat is deployed to the field.  To find how the insect can overcome wheat resistance, the genes that are responsible for the Hessian fly to attack wheat need to be identified.  This work is to provide a detailed map of the Hessian fly genome with specific markers.  The work provides a foundation for identification of specific Hessian fly genes and for whole genome sequencing.
                                Technical Abstract: The Hessian fly (Mayetiola destructor) is an important insect pest of wheat and an experimental organism for studies of plant-insect interactions.  It has tractable genetics, polytene chromosomes, a relatively small genome (158 Mb), and shares a gene-for-gene relationship with wheat.  To improve its capacity as a model plant-gall forming insect, an FPC-based genome wide physical map of the Hessian fly was constructed and anchored to the polytene chromosomes of the insect.  Bacterial artificial chromosome (BAC) clones corresponding to 10.5-fold coverage of the Hessian fly genome were fingerprinted, using high-resolution capillary polyacrylamide gel electrophoresis, and end sequenced.  The reliability of the FPC assembly was tested using fluorescence in situ hybridization (FISH) to co-localize two BAC clones from each of the 196 longest contigs on the polytene chromosomes.  To assess contig distribution, 70 additional contigs were FISH mapped.  The FISH mapped contigs were evenly distributed and covered 60% of the genome (95,668 kb).  Only 3.63% of the BAC-end sequence was composed of transposable elements, helicases, ribosomal repeats, simple sequence repeats, and sequences of low complexity.  However, 14.51% of the BES was comprised of multi-copy gene sequences.  This physical map provides the foundation for high-resolution genetic mapping, map-based cloning, and assembly of complete genome sequencing data.  The Hessian fly BAC clone assembly, and the names and positions of the BAC clones used in the FISH experiments are freely available at http://genome.purdue.edu/WebAGCoL/Hfly/WebFPC/.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov