U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 close



Research
                Research Home
                            National Programs
                            Research Projects
                            Scientific Manuscripts
                            International Engagement
                            Scientific Software/Models
                            Databases and Datasets
                            Office of Scientific Quality Review
            Media
                News & Features
                            Multimedia
                            Briefing Room
                            Down on the Farm
                            Press Room
                            Science in Your Shopping Cart
                            Scientific Discoveries
                            Tellus
                            Under the Microscope
            About ARS
                About ARS Home
                            Staff Directory
                            Labs and Research Centers (Map)
                            Headquarter Offices
                            Organizational Chart
                            Employee Services (REE Employees Only)
                            Office of Outreach, Diversity, and Equal Opportunity
            Work With Us
                Work With Us Home
                            ARS Vacancies at USAJOBS
                            Careers at ARS Information
                            Scientific Collaborations
                            Extramural Agreements
                            1890 Faculty Research Sabbatical ProgramSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Sarmento, Lucianaitem
                                            Pantin Jackwood, Maryitem
                                            Kapczynski, Darrellitem
                                            Swayne, Daviditem
                                            Afonso, Claudio
  Submitted to: Biologicals
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 12/20/2007
                                    Publication  Date: 3/20/2008
                                    Citation: Sarmento, L., Pantin Jackwood, M.J., Kapczynski, D.R., Swayne, D.E., Afonso, C.L. 2008. Immediate early responses of avian tracheal epithelial cells to infection with highly pathogenic avian invluenza virus. Biologicals. 132:175-183.Interpretive Summary: Avian influenza is a major viral disease of poultry that occurs worldwide and leads to significant financial losses.  Losses are caused by the death of the birds infected with the virus, culling of infected and likely infected birds to control the spread of the virus, and from restrictions on export of poultry and poultry products from the region of the outbreak.  Currently live attenuated vaccines are not used because of significant concerns over stability and safety.  In order to improve our ability to protect naïve birds from infection and to create live attenuated vaccines basic knowledge on the early host responses and the mechanism of innate immunity are needed.  Here we describe the establishment of chicken and duck tracheal epithelial cell culture system and the study of early responses to infection using microarrays as new tools to identify molecular changes that occur at mucosal sites of early AIV infection.
                                Technical Abstract: Highly pathogenic (HP) avian influenza viruses (AIV) present an ongoing threat to the world poultry industry.  In order to develop new AIV control strategies it is necessary to understand the underlying mechanism of viral infection at mucosal respiratory sites.  Chicken and duck tracheal epithelial cells systems (TEC) were developed to study early host responses to AIV infection on TEC.  Infection of 2 week-chickens and ducks with the highly pathogenic AIV H5N1 Ck/Hong Kong/220/97 and Egret/Hong Kong/757.2/02 viruses together with TEC early responses to infection suggest the induction of differential innate immune responses.  Growth curves indicated that although chicken and ducks TEC supported viral replication and re-infection, the capacity of the two viruses to replicate was not equal.  A 42K gene chicken microarray system used to characterize differences in gene expression between chicken tracheal epithelial cells infected with these two highly pathogenic AIV identified expression of virus-specific molecular markers.  The existence of dissimilar patterns of host gene expression as early as six hours post infection suggest that the differential growth characteristics of the two highly pathogenic AIV in tracheal epithelial cells is preceded by distinct host responses.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov