U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 close



Research
                Research Home
                            National Programs
                            Research Projects
                            Scientific Manuscripts
                            International Engagement
                            Scientific Software/Models
                            Databases and Datasets
                            Office of Scientific Quality Review
            Media
                News & Features
                            Multimedia
                            Briefing Room
                            Down on the Farm
                            Press Room
                            Science in Your Shopping Cart
                            Scientific Discoveries
                            Tellus
                            Under the Microscope
            About ARS
                About ARS Home
                            Staff Directory
                            Labs and Research Centers (Map)
                            Headquarter Offices
                            Organizational Chart
                            Employee Services (REE Employees Only)
                            Office of Outreach, Diversity, and Equal Opportunity
            Work With Us
                Work With Us Home
                            ARS Vacancies at USAJOBS
                            Careers at ARS Information
                            Scientific Collaborations
                            Extramural Agreements
                            1890 Faculty Research Sabbatical ProgramSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Obrien, Celiaitem
                                            Jenkins, Mark
  Submitted to: Journal of Parasitology
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 10/17/2006
                                    Publication  Date: 6/1/2007
                                    Citation: Obrien, C.N., Jenkins, M.C. 2007. A rapid method for production of high numbers of purified Cryptosporidium parvum oocysts. Journal of Parasitology. 93:434-436.Interpretive Summary: Cryptosporidiosis is an intestinal disease of humans and animals caused by the protozoan parasite Cryptosporidium parvum.  The disease is prevalent in the young because of an incompletely developed immune system.  Because of this lack of immunity, young calves are used to propagate Cryptosporidium oocysts.  Large numbers of purified oocysts are necessary to study the proteins of the parasite in order to understand enzymatic processes and develop diagnostic tools.  Purifying oocysts from fecal material can be a time consuming, expensive process resulting in low yields and purity.  This rapid procedure combines a sodium chloride flotation step followed by an ether extraction to recover a large number of Cryptosporidium oocysts devoid of contaminating fecal debris or bacteria.  The high yield of the oocyst preparation minimizes the number of propagations necessary and the high purity ensures the protein assays are devoid of microbe contamination.
                                Technical Abstract: Most procedures that have been described for purifying Cryptosporidium parvum oocysts are designed to either identify the parasites in clinical specimens or isolate oocysts from a small volume of feces from infected animals.  The present study describes a rapid method for purifying high numbers of C. parvum oocysts from feces of infected calves that are virtually free of contaminating bacteria and yeast.  This procedure regularly yields > 109 purified C. parvum oocysts within 1-2 days of feces collection.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov