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Title:   Author  item
                                            ELLWOOD, SIMON - MURDOCH UNIV., AUSTRALIAitem
                                            KAMPHUIS, LARS - MURDOCH UNIV., AUSTRALIAitem
                                            PFAFF, THEO - MURDOCH UNIV., AUSTRALIAitem
                                            OLIVER, RICHARD - MURDOCH UNIV., AUSTRALIAitem
                                            Samac, Deborah

                                                         - *Debby*item
                                            FOSTER-HARTNETT, DAWN - UNIVERSITY OF MINNESOTAitem
                                            TIVOLI, BERNARD - INRA/AGROCAMPUS, FRANCEitem
                                            ONFROY, CAROLINE - INRA/AGROCAMPUS, FRANCEitem
                                            MOUSSART, ANNE - INRA/AGROCAMPUS, FRANCE
  Submitted to: Book Chapter
                                    Publication Type: Book / Chapter
                                    Publication Acceptance Date: 7/1/2006
                                    Publication  Date: 12/8/2006
                                    Citation: Ellwood, S., Kamphuis, L., Pfaff, T., Oliver, R., Samac, D.A., Foster-Hartnett, D., Tivoli, B., Onfroy, C., Moussart, A. 2006. Inoculation and growth with foliar pathogenic fungi. In: The Medicago truncatula Handbook. The Samuel Roberts Noble Foundation, Ardmore, OK. Available: http://www.noble.org/MedicagoHandbook/.Interpretive Summary:
                                Technical Abstract: This chapter describes methods for evaluating responses of Medicago truncatula to foliar plant-pathogenic fungi. Growth of plants, preparation of inoculum, incubation conditions, and disease assessment are described for five necrotrophic pathogens (Ascochyta lentis, Ascochyta rabiei, Colletotrichum trifolii, Mycosphaerella pinodes, Phoma medicaginis) and one biotroph (Eysiphe pisi). For several diseases both whole plant and detached leaf methods have been developed. For each assay, check plants and their responses to the pathogen are listed. Methods for re-isolation of the pathogen from diseased tissue and storage of cultures are also detailed. For several diseases, methods for staining and visualization of the fungi in plant material are described.


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