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 Corn Insects and Crop Genetics Research: Ames, IA
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Title:   Author  item
                                            Wise, Rogeritem
                                            Halterman, Dennisitem
                                            WEI, FUSHENG - IOWA STATE UNIVERSITYitem
                                            WING, ROD - CLEMSON UNIVERSITYitem
                                            ZHOU, FASONG - SAINSBURY LABORATORYitem
                                            SCHULZE-LEFERT, PAUL - SAINSBURY LABORATORY
  Submitted to: Keystone Symposia
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 1/30/2001
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: The barley Mla locus confers multiple resistance specificities to the powdery mildew fungus, Blumeria (=Erysiphe) graminis f. sp. hordei. Interspersed within the 240-kb Mla complex are three families of resistance gene homologs (RGHs).  Probes from the Mla-RGH1 family were used to identify three classes of cDNAs.  The first is predicted to encode a full-  length CC-NBS-LRR protein and the other two are truncated variants. Utilizing a cosmid that contains a gene corresponding to the full-length candidate cDNA, a single-cell expression assay was used to demonstrate complementation of AvrMla6-dependent resistance to B. graminis in barley and wheat.  This assay was also used to substantiate previous genetic data that the Mla6 allele requires the signaling pathway component, Rar1, for function.  Comparison of Mla6 and the Rar1-independent Mla1 protein reveals 91.2% sequence identity, demonstrating that highly related CC-NBS-LRR proteins encoded by alleles at the Mla locus can dictate different powdery mildew recognition specificities and require distinct signaling components. Research supported by USDA-NRI/CGP grant 98-35300-6170.


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