U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 U.S. Dairy Forage Research Center: Madison, WI
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                                    Software
                People

                    Staff Listing
                                    Careers


        


                    Archives
                        Presentations
                                    World Dairy Expo
                                    Newsletters and Research Summaries
                    Resources
                        General Resources
                                    Books
                                    Teacher's Resources
                                    New Research Dairy Updates
                                    Integrated Research at USDFRCSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area Madison, Wisconsin U.S. Dairy Forage Research Center Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications
- IACUC Summaries - Milwaukee, WI Aquaculture
- IACUC Summaries - US Dairy Forage Research Center

Title:   Author  item
                                            Hall, Mary Bethitem
                                            Weimer, Paul
  Submitted to: Journal of Dairy Science
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 3/15/2006
                                    Publication  Date: 7/12/2006
                                    Citation: Hall, M., Weimer, P.J. 2006. Implications of a carbon balance study: organic acid and protein supplies change with fermentable carbohydrate:protein ratio [abstract]. Journal of Dairy Science. 89:Suppl 1:404.Interpretive Summary:
                                Technical Abstract: Three concentrations of sucrose (Suc, trt) with one of isolated neutral detergent fiber (isolNDF) from bermudagrass were fermented together in vitro with rumen inoculum to evaluate the effects of Suc concentration on partition of carbon (C) in Suc into fermentation products. Nitrogen sources were inoculum, isolNDF and casein hydrolysate and NH4HCO3 in the medium. Yield of C in fermentation products [organic acids (OA:acetate, propionate, butyrate, lactate), microbial crude protein (MCP), CO2, CH4, glycogen] from C from fermented Suc was evaluated at peak MCP production from Suc (detected at 4 to 8 h of fermentation; Suc almost entirely fermented at this point). Yield of total products, MCP and OA decreased or tended to decrease linearly with increasing Suc. This may be a function of decreased catabolic efficiency of the microbes with increasing Suc as evidenced by increasing yields of lactate, but increased energy spilling is also a possibility. Values > 1.0 indicate incorporation of C from the medium, likely from the inoculum and casein hydrolysate. When corrected for estimates of microbial cell C not in MCP, product C from Suc for the greatest Suc trt was 0.96. Even with C from MCP excluded, yield of C in other products per unit of Suc utilized still differed among trt (1.03, 0.77, and 0.61 from lowest to greatest Suc inclusion; linear effect of Suc, P = 0.017; SED = 0.054). In this study, the ratio of available protein to fermentable Suc decreased with increasing Suc. Published studies report increased yield of MCP in vitro and increased ruminal acid concentrations in vivo as the ratio of degradable protein:fermented carbohydrate was increased. Results of this C balance support the premise that the ratio of available N to Suc and direct effects of Suc concentration altered partitioning of C into products. This has implications for prediction of ruminal pH and supply of nutrients from ruminal fermentation.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov