U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Food and Feed Safety Research: College Station, TX
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Plains Area College Station, Texas Southern Plains Agricultural Research Center Food and Feed Safety Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            He, Louisitem
                                            Genovese, Kenneth

                                                         - *Ken*item
                                            Kogut, Michael

                                                         - *Mike*
  Submitted to: Avian Immunology Research Group Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 6/20/2006
                                    Publication  Date: 10/21/2006
                                    Citation: He, H., Genovese, K.J., Kogut, M.H. 2006. Nitric oxide synthesis and cytokine gene expression in chicken monocytes stimulated with CpG-ODN and double-stranded RNA [abstract]. In: Proceedings of 09th Avian Immunology Research Group Meeting, October 21-24, 2006, Paris, France. p. 29.Interpretive Summary:
                                Technical Abstract: Toll-like receptors (TLRs) recognize microbial components and initiate innate immune responses that control microbial infections.  We have investigated the innate immune response of chicken monocytes to bacterial CpG-motif containing oligodeoxydinucleotide, CpG-ODN, and the analog of viral double-stranded RNA, poly I:C, by measuring the induction of NO synthesis and cytokine gene expression.  Our results show ligands poly I:C and CpG-ODN of the TLR3 and TLR9, respectively, synergized the induction of NO in chicken monocytes.  When stimulated separately, CpG-ODN induced NO production in the chicken monocytes; whereas, poly I:C stimulated very little NO production.  In combination, CpG-ODN and poly I:C synergize the effect on NO synthesis, which results in significantly higher level of NO production in chicken monocytes.  This synergistic effect of CpG ODN-ODN and poly I:C was also observed on the expression of IL-1beta and IL-12 mRNA.  In addition, IL-18 gene expression was differential induced by poly I:C stimulation of monocytes, but not by CpG-ODN stimulation.  Those results demonstrate differential roles for TLR3 and TLR9 in signaling immune responses to bacterial and viral infections in chicken monocytes as well as a synergistic interaction between the two signaling pathways.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov