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Title:   Author  item
                                            Goodwin, Stephen

                                                         - *Steve*
  Submitted to: Phytopathology
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 6/15/2005
                                    Publication  Date: 7/31/2005
                                    Citation: Goodwin, S.B. 2005. Variability of microsatellite markers within and between populations of Mycosphaerella graminicola in the central United States. Phytopathology. 95:535-536.Interpretive Summary:
                                Technical Abstract: The population genetic structure of Mycosphaerella graminicola, the fungal pathogen responsible for worldwide epidemics of Septoria tritici blotch of wheat, is influenced by many environmental and genetic factors.  Of particular interest are the differing factors inherent in the hard, red spring wheat of North Dakota and Minnesota in relation to the soft, red winter wheat grown in Indiana and Ohio.  By testing newly available microsatellites in gene coding regions, it may be possible to ascertain if microsatellite analysis is adequate for determining genetic differentiation.  If so, subtle population differentiations previously unrecognized could be elucidated.  In preliminary tests of seven microsatellites, two to three alleles per locus were generally seen, most with little to no genetic variation detected between potential subpopulations.  However, allele frequencies at two microsatellite loci were sufficiently different statistically to warrant additional experimentation.  Further analyses with these microsatellites may help to test the effect of population structure on pathogenic differentiation, and to estimate the levels of migration between populations on hard spring versus soft winter wheat in the central United States.


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