U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 close



Research
                Research Home
                            National Programs
                            Research Projects
                            Scientific Manuscripts
                            International Engagement
                            Scientific Software/Models
                            Databases and Datasets
                            Office of Scientific Quality Review
            Media
                News & Features
                            Multimedia
                            Briefing Room
                            Down on the Farm
                            Press Room
                            Science in Your Shopping Cart
                            Scientific Discoveries
                            Tellus
                            Under the Microscope
            About ARS
                About ARS Home
                            Staff Directory
                            Labs and Research Centers (Map)
                            Headquarter Offices
                            Organizational Chart
                            Employee Services (REE Employees Only)
                            Office of Outreach, Diversity, and Equal Opportunity
            Work With Us
                Work With Us Home
                            ARS Vacancies at USAJOBS
                            Careers at ARS Information
                            Scientific Collaborations
                            Extramural Agreements
                            1890 Faculty Research Sabbatical ProgramSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            SHINI, J - AVICORE BIOTECH KOREAitem
                                            KIM, H - SEOUL NTL UNIV KOREAitem
                                            LIM, D - SEOUL NTL UNIV KOREAitem
                                            JEON, M - SEOUL NTL UNIV KOREAitem
                                            HANI, B - AVICORE BIOTECH KOREAitem
                                            PARK, T - SEOUL NTL UNIV KOREAitem
                                            KIM, J - CHANGWON NTL UNIV KOREAitem
                                            Lillehoj, Hyunitem
                                            CHO, B - MIRYANG UNIV KOREAitem
                                            HAN, J - SEOUL NTL UNIV KOREA
  Submitted to: Animal Genetics
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 9/2/2006
                                    Publication  Date: 10/2/2006
                                    Citation: Shini, J.H., Kim, H., Lim, D., Jeon, M., Hani, B.K., Park, T.S., Kim, J.K., Lillehoj, H.S., Cho, B.W., Han, J.Y. 2006. Analysis of chicken embryonic gonad ests. Animal Genetics 37:85-86.Interpretive Summary: In this paper, ARS scientists collaborated with scientists at Seoul National University in South Korea to develop a geneomic resource from chicken embryonic gonads.  Total 11,842 cDNA clones from the embryonic gonad cDNA library were sequenced and their gene sequences submitted to NCBI dbEST public database. This genetic resource will provide important sequence information and genes for scientists who study embryo gonad development in poultry.
                                Technical Abstract: We have sequenced 11,842 cDNA clones from the embryonic gonad cDNA library to generate 10,294 sequences. The EST data described in this paper have been submitted to the NCBI dbEST under accession numbers CV852525 CV862818. The unique sequences of the EST data resulted in a total of 4,384 sequences with 934 contigs and 3,450 singlets. The average number of ESTs per contig was about 4.3. The number of putative novel transcripts obtained by comparison with GgGI (Release 10.0) totaled 1,215 sequences with 131 contigs and 1,084 singlets. Among the putative novel transcripts, about 10% of sequences were blast best hits in chicken. Functional prediction and gene ontology distribution on the basis of sequence similarity searches against the nonredundant protein database of the NCBI. Interestingly, Pearson=s chi square test of independence of 2nd level GO terms between gonad and novel transcripts indicates varieties of extracellular proteins may be involved in the gene expression of early embryonic gonads. In addition, transporter activity, obsolete molecular functions, and behavior terms were higher in the novel transcripts than in the gonad set.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov