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Title:   Author  item
                                            Grunwald, Niklaus

                                                         - *Nik*item
                                            PROSPERO, SIMONE - INRAitem
                                            HANSEN, EVERETTE - OREGON STATE UNIVERSITYitem
                                            TYLER, BRETT - VIRGINIA TECHitem
                                            LAMOUR, KURT - UNIVERSITY OF TENNESSEE
  Submitted to: Mycological Society of America
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 4/6/2006
                                    Publication  Date: 8/1/2006
                                    Citation: Grunwald, N.J., Prospero, S., Hansen, E., Tyler, B., Lamour, K. 2006. Population divergence in Phytophthora ramorum. Mycological Society of America Abstracts. p.197.Interpretive Summary:
                                Technical Abstract: Phytophthora ramorum, causal agent of Sudden Oak Death on oaks and Ramorum blight on ornamentals such as rhododendron, is an emerging pathogen with significant impact on both natural oak forest ecosystems and the nursery industry. The pathogen was simultaneously discovered in Germany and California. Recent studies based on AFLP established that the US population was clonal. We screened the genome of P. ramorum for simple sequence repeat and single nucleotide polymorphisms loci. While trinucloeotide loci only distinguished between the EU and U.S. clones, tetranucleotide repeats were found to be highly informative for locally evolving populations. We also developed an Affymetrix microarray with 880 SNPs. These assays were applied to the U.S. and EU populations as well as recently discovered novel clones. SNP and SSR discovery is ongoing. All these markers indicate that both the European and North American populations are reproductively isolated and have gone through a bottleneck. There currently is no evidence for sexual recombination. Novel genotypes detected in the U.S. likely originated through migration.


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