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Title:   Author  item
                                            Waters, Wadeitem
                                            Palmer, Mitchellitem
                                            Pesch, Bruceitem
                                            Olsen, Stevenitem
                                            WANNEMUEHLER, M - IOWA STATE UNIV., AMESitem
                                            Whipple, Diana
  Submitted to: International Conference on Mycobacterium bovis
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 8/14/2000
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: Despite highly successful eradication efforts in several countries, Mycobacterium bovis infection of cattle remains a significant health concern worldwide.  Immune mechanisms of resistance to and/or clearance of M. bovis infection of cattle, however, are unclear.  Recent studies have provided evidence supporting a role for CD4+, CD8+, and gamma/delta TCR+ T  cells in the response of cattle to M. bovis.  In the present study, we utilized a flow cytometric-based proliferation assay to determine the relative contribution of individual lymphocyte subsets in the response to M. bovis infection and/or sensitization with mycobacterial purified protein derivative (PPD).  Peripheral blood mononuclear cells (PBMC) from M. bovis-infected cattle proliferated in response to in vitro stimulation with PPD.  CD4+ T cells and gamma/delta TCR+ cells were the predominate subsets of lymphocytes responding.  Gamma/delta TCR+ cells also  proliferated in non-stimulated cultures; however, the gamma/delta TCR+ cell proliferative response of infected cattle was significantly (P less than 0.05) greater in PPD-stimulated cultures as compared to non- stimulated cultures.  Intradermal injection of PPD for comparative cervical testing (CCT) induced a boost in the CD4+ cell, but not gamma/delta TCR+ cell, proliferative response of infected cattle. Injection of PPD for CCT did not, however, elicit a proliferative response in non-infected cattle.  These data indicate that CD4+ and gamma/delta TCR+ cells of M. bovis-infected cattle proliferate in a recall response to PPD and that the CD4+ cell response is boosted by intradermal injection with PPD for CCT.


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