U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Corn Insects and Crop Genetics Research: Ames, IA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Database Projects
                        MaizeGDB
                                    SoyBase
                                    PeanutBase
                                    Legume Information System
                                    Maize Newsletter
                                    Maize Mutant Phenotypes
                                    Maize Genetics Conference
                                    Shiny Soybase CNV
                                    Shiny Soybase SNP
                                    Common Bean and Alfalfa - PvGEA
                                    Common Bean and Alfalfa - AGEDSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area Ames, Iowa Corn Insects and Crop Genetics Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            CALDO, RICO - IOWA STATE UNIVERSITYitem
                                            NETTLETON, DAN - IOWA STATE UNIVERSITYitem
                                            Wise, Roger
  Submitted to: Plant and Animal Genome Conference Proceedings
                                    Publication Type: Proceedings
                                    Publication Acceptance Date: 1/10/2004
                                    Publication  Date: 1/10/2004
                                    Citation: Caldo, R.A., Nettleton, D., Wise, R.P. 2004. Interplay of non-specific and specific modulation patterns of barley transcriptional responses during powdery mildew infection. Plant and Animal Genome Conference Proceedings. XII Conference. p. 793.Interpretive Summary:
                                Technical Abstract: Large-scale parallel expression analysis was conducted to elucidate Mla-specified responses to powdery mildew infection using 22K Barley1 GeneChip probe arrays. Our goal was to identify genes that are differentially expressed in incompatible (resistant) vs. compatible (susceptible) interactions. A split-split-plot design with 108 experimental units (3 replications x 2 isolates x 3 genotypes x 6 time points) was used to profile near-isogenic lines containing the Mla1, Mla6, and Mla13 resistance specificities in response to inoculation with the Blumeria graminis f. sp. hordei (Bgh) isolates 5874 (AvrMla1, AvrMla6) and K1 (AvrMla1, AvrMla13). F-tests associated with specific contrasts were used as part of a mixed linear model analysis to identify genes whose transcriptional pattern differed significantly over the course of powdery mildew infection. Twenty-two genes, of which half were unknown function, showed highly similar patterns at the very early time points of infection, but were markedly different at 16 to 32 hours after inoculation (hai). These transcriptional responses are correlated with general conidiospore germination and appressorial growth prior to 16 hai, in addition to the onset of Mla-specified resistance that occurs after attempted penetration of epidermal cells in incompatible interactions. Based on the microarray data, we propose a model for the interplay of non-specific and specific recognition of powdery mildew invasion in modulating barley transcriptional responses. Research supported by USDA-IFAFS grant no. 2001-52100-11346.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov