U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 close



Research
                Research Home
                            National Programs
                            Research Projects
                            Scientific Manuscripts
                            International Engagement
                            Scientific Software/Models
                            Databases and Datasets
                            Office of Scientific Quality Review
            Media
                News & Features
                            Multimedia
                            Briefing Room
                            Down on the Farm
                            Press Room
                            Science in Your Shopping Cart
                            Scientific Discoveries
                            Tellus
                            Under the Microscope
            About ARS
                About ARS Home
                            Staff Directory
                            Labs and Research Centers (Map)
                            Headquarter Offices
                            Organizational Chart
                            Employee Services (REE Employees Only)
                            Office of Outreach, Diversity, and Equal Opportunity
            Work With Us
                Work With Us Home
                            ARS Vacancies at USAJOBS
                            Careers at ARS Information
                            Scientific Collaborations
                            Extramural Agreements
                            1890 Faculty Research Sabbatical ProgramSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            HONG, YEONGitem
                                            Lillehoj, Hyunitem
                                            DALLOUL, RAMI - USDA ARS ANRI BELTSVILLEitem
                                            Miska, Kateitem
                                            LEE, SUNG - USDA ARS ANRI BELTSVILLE
  Submitted to: Coccidiosis International Conference Proceedings
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 8/5/2005
                                    Publication  Date: 9/15/2005
                                    Citation: Hong, Y.H., Lillehoj, H.S., Dalloul, R.A., Miska, K.B., Lee, S.H. 2005. Molecular cloning and expression profiles of chicken nk-lysin during eimeria infections.. Coccidiosis International Conference Proceedings, p. 153.Interpretive Summary:
                                Technical Abstract: NK-lysin is known to have antibacterial and antitumor activities and is expressed by NK cells as well as T lymphocytes.  In a previous report, the contig 171 (NK-lysin like sequence), composed of 87 ESTs, occurred with the highest prevalence in an Eimeria-induced intestinal cDNA library.  This study was conducted to clone, express, and characterize contig 171 as the chicken NK-lysin, which consists of an 868 bp DNA sequence and an open reading frame of 140 amino acids with a predicted molecular mass of 15.2 kDa.  Comparison of deduced amino acid sequences showed less than 20% identity to granulysin and other mammalian NK-lysins.  When infected with Eimeria maxima, chicken total IELs, CD4+, and CD8+ IELs showed higher expression levels of NK-lysin mRNA than other IEL subpopulations.  Upon infections with three Eimeria species, chicken NK-lysin RNA expression level to GAPDH showed differential and interesting patterns.  Chicken NK-lysin was expressed into COS-7 cells and its antitumor activity was demonstrated in killing LSCC-RP9 macrophage transformed cells.  Further, chicken NK-lysin has sequence similarity to other mammalian cDNA with cytotoxic activity to tumor cells.  In these studies, we have cloned and characterized chicken NK-lysin and studied its expression patterns in mRNA of Eimeria-infected birds.  As in other animals, chicken NK-lysin may play an important role in antimicrobial defenses against enteric pathogens such as Eimeria and salmonella, especially at the early stages of such infections.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov