U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Global Change and Photosynthesis Research: Urbana, IL
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area Urbana, Illinois Global Change and Photosynthesis Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Ainsworth, Elizabeth

                                                         - *Lisa*item
                                            GILLESPIE, KELLY - UNIVERSITY OF ILLINOIS
  Submitted to: Nature Protocols
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 2/9/2007
                                    Publication  Date: 4/12/2007
                                    Citation: Ainsworth, E.A., Gillespie, K.M. 2007. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent. Nature Protocols.  2:875-877.Interpretive Summary: Non-structural phenolic compounds provide a variety of functions to plants, including acting as antioxidants. We describe a microplate-adapted colorimetric total phenolics assay, which utilizes Folin-Ciocalteu (F-C) reagent. This assay is described with two other assays for measuring the antioxidant capacity of leaf tissue extracts.  These assays provide scientists with high-throughput tools for screening the response of plants to environmental stress.
                                Technical Abstract: Non-structural phenolic compounds provide a variety of functions to plants, including acting as antioxidants. We describe a microplate-adapted colorimetric total phenolics assay, which utilizes Folin-Ciocalteu (F-C) reagent. The F-C assay relies on the transfer of electrons in alkaline medium from phenolic compounds to phosphomolybdic/phosphotungstic acid complexes, which are determined spectroscopically at 765 nm. While the electron transfer reaction is not specific for phenolic compounds, the described extraction procedure eliminates ~85% of ascorbic acid and other potentially interfering compounds. The assay is performed in microcentrifuge tubes and assessed in a 96-well plate reader. At least 64 samples can be processed in one day.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov