U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 close



Research
                Research Home
                            National Programs
                            Research Projects
                            Scientific Manuscripts
                            International Engagement
                            Scientific Software/Models
                            Databases and Datasets
                            Office of Scientific Quality Review
            Media
                News & Features
                            Multimedia
                            Briefing Room
                            Down on the Farm
                            Press Room
                            Science in Your Shopping Cart
                            Scientific Discoveries
                            Tellus
                            Under the Microscope
            About ARS
                About ARS Home
                            Staff Directory
                            Labs and Research Centers (Map)
                            Headquarter Offices
                            Organizational Chart
                            Employee Services (REE Employees Only)
                            Office of Outreach, Diversity, and Equal Opportunity
            Work With Us
                Work With Us Home
                            ARS Vacancies at USAJOBS
                            Careers at ARS Information
                            Scientific Collaborations
                            Extramural Agreements
                            1890 Faculty Research Sabbatical ProgramSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Liu, Ge

                                                         - *George*item
                                            Van Tassell, Curtis

                                                         - *Curt*item
                                            Sonstegard, Taditem
                                            Alexander, Leesonitem
                                            Keele, Johnitem
                                            MATUKUMALLI, L - GEORGE MASON UNIVERSITYitem
                                            Smith, Timothy

                                                         - *Tim*item
                                            Gasbarre, Louis
  Submitted to: International Symposium on Animal Genomics for Animal Health
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 9/20/2007
                                    Publication  Date: 10/23/2007
                                    Citation: Liu, G., Van Tassell, C.P., Sonstegard, T.S., Alexander, L.J., Keele, J.W., Matukumalli, L.K., Smith, T.P., Gasbarre, L.C. 2007. Bovine copy number variation and its implication in animal health. [ abstract]. International Symposium on Animal Genomics for Animal Health. pp. 32.Interpretive Summary:
                                Technical Abstract: Recently it has become apparent that previously unappreciated genomic structural variation, including copy number variations (CNV), contributes significantly to individual health and disease in humans and rodents. As a complement to the bovine HapMap project, we initiated a systematic study of the CNV within the same cattle population using array comparative genomic hybridization (array CGH). Oligonucleotide CGH arrays were designed and fabricated to cover all chromosomes with an average interval of 6 kb using the version 3 of bovine genome assembly. In the initial screening, three Holstein bulls were selected to represent major branches of the Holstein breed with some maternal linkages between branches. Dual-label hybridizations were performed using either L1 Dominette 01449 or L1 Domino 99375 as reference. The CNV were represented by gains and losses of normalized fluorescence intensities relative to the reference. Our data, for the first time, demonstrated that significant amounts of CNV exist in the cattle; many CNV are common among diverse cattle individuals; and array CGH is an effective way to detect bovine CNV even within a breed. Selected CNV are being confirmed by independent methods using Q-PCR and/or FISH. We are also investigating the frequency, pattern and impact of such CNV in cattle to investigate their utility in improving selection for health, well-being and productive efficiency of cattle. Our strategy based on genome higher-order architecture variation is a powerful approach to generate resources for the identification of novel genomic variation and candidate genes for important economic traits.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov