U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Genetics and Sustainable Agriculture Research: Mississippi State, MS
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                                    Software
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Southeast Area Mississippi State, Mississippi Crop Science Research Laboratory Genetics and Sustainable Agriculture Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Saha, Sukumaritem
                                            RASKA, D - TEXAS A&M UNIVERSITYitem
                                            Jenkins, Johnieitem
                                            McCarty, Jackitem
                                            STELLY, D - TEXAS A&M UNIVERSITY
  Submitted to: International Cotton Genome Initiative Workshop
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 9/18/2006
                                    Publication  Date: 9/18/2006
                                    Citation: Saha, S., Raska, D.A., Jenkins, J.N., McCarty Jr., J.C., Stelly, D.M. 2006. Interspecific chromosome substitution lines in upland cotton improvement. International Cotton Genome Initiative Workshop. p. 11.Interpretive Summary:
                                Technical Abstract: Despite the economic importance of Upland cotton, the genetic base of US cultivars and elite germplasm is very narrow.  Two of the primary impediments to  genetic improvement of cotton are:  1) insufficient information about genes that control important traits and 2) under-utilization of diverse germplasm. Wide-cross introgression typically requires considerable time and effort to circumvent and overcome biological, genetic and cytogenetic obstacles, and the choice of breeding strategies to introgress germplasm can greatly influence success.  Conventional methods of interspecific introgression into cotton typically entail inbreeding immediately after hybridization or after a few backcrosses.  A complementary approach is alien chromosome substitution. The overall goal of this project is to develop chromosome substitution lines from the other tetraploid species into Gossypium hirsutum.   Development of each alien species chromosome substitution line involves three stages:  [1] development of the respective TM-1-like hypoaneuploid stock, [2] use of the cytogenetic stock as recurrent seed parent in a recurrent backcrossing program to  create a monosomic or monotelodisomic substitution stock, followed by [3] inbreeding to recover  a euploid disomic substitution line. TM-1, the highly inbred Upland cotton (G. hirsutum) genetic standard, serves as the recurrent parent.  The backcrossed chromosome substitution lines are expectedly quasi-isogenic to TM-1 and to each other, except that each line differs by the replacement of a specific homologous pair of chromosomes or chromosome segments from the donor alien species.  When possible, the cytogenetic and genetic constitution of the disomic lines is confirmed by cytological analysis and chromosome-specific SSR markers. We released 17 backcrossed (BC5) G. barbadense chromosome or chromosome arm substitution lines (CS-B).  These CS-B lines are providing important information about the association of G. barbadense substituted chromosome association with important fiber and agronomic traits.  They have also been used to discover their merits in Upland cultivar improvement.  For high-resolution analysis of QTLs, we are using many of the CS-B lines in development of chromosome-specific recombinant inbred lines.  In addition to the disomic CS-B lines, we also have developed 13 monosomic and 32 monotelodisomic substitution stocks (BC0F1) for different chromosomes or chromosome arms of G. tomentosum and G. mustelinum. Research is also underway to use these for disomic chromosome substitution line development.  These new genomic resources will complement the conventional types of interspecific introgression in Upland cotton improvement.  All of these materials will provide additional tools in genomic analysis and genetic improvement of Upland cotton.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov