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Title:   Author  item
                                            Dowd, Patrickitem
                                            Johnson, Ericitem
                                            Pinkerton, Terrence
  Submitted to: National Entomological Society of America Annual Meeting
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 12/12/2006
                                    Publication  Date: 12/10/2006
                                    Citation: Dowd, P.F., Johnson, E.T., Pinkerton, T.S. 2006. Evidence for hexosaminidase as an insect resistance factor of plants [abstract]. National Entomological Society of America Annual Meeting. Paper No. 1063.Interpretive Summary:
                                Technical Abstract: Chitin is a potential target for insect resistance mechanisms in plants, and hexosaminidase is one enzyme that can degrade chitin.  Commercial preparations of a hexosaminidase caused significant mortality of neonate fall armyworm larvae at 75 ppm compared to buffer controls.  When an Arabidopsis gene potentially coding for hexosaminidase was introduced into stable corn callus and two different lines of corn that were regenerated, resistance to fall armyworms was significantly enhanced compared to nonexpressing controls.  Significantly higher mortality and/or reductions in growth rates occurred in several examples.  Enhanced resistance in the transgenic lines appeared associated with the presence of hexosaminidase detected enzymatically with a model substrate.


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