U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Infectious Bacterial Diseases Research: Ames, IA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area Ames, Iowa National Animal Disease Center Infectious Bacterial Diseases Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Waters, Wadeitem
                                            Palmer, Mitchellitem
                                            Thacker, Tyleritem
                                            PAYEUR, JANET - APHISitem
                                            MINION, F - IOWA STATE UNIVitem
                                            GREENWALD, R - CHEMBIO DIAG. SYSTEMSitem
                                            ESFANDIARI, J - CHEMBIO DIAG. SYSTEMSitem
                                            ANDERSEN, P - STATEN SERUM INST., DENMAitem
                                            MCNAIR, J - VET. SCI. DIV., BELFASTitem
                                            POLLOCK, J - VET. SCI. DIV., BELFAST
  Submitted to: International Conference on Mycobacterium bovis
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 8/22/2005
                                    Publication  Date: 8/22/2005
                                    Citation: Waters, W.R., Palmer, M.V., Thacker, T.C., Payeur, J.B., Minion, F.C., Greenwald, R., Esfandiari, J., Andersen, P., Mcnair, J., Pollock, J.M. 2005. Mycobacterium Kansasii Infection of Cattle Confounds Interpretation of Tuberculosis Diagnostic Tests [abstract]. International Conference on Mycobacterium bovis. p. 26.Interpretive Summary:
                                Technical Abstract: 

Cross-reactive responses elicited by exposure to non-tuberculous mycobacteria often confound interpretation of traditional ante-mortem bovine tuberculosis (TB) tests. Use of specific proteins such as ESAT-6, CFP-10, and MPB83 may improve the specificity of these tests in areas where exposure to environmental non-tuberculous mycobacteria is common. However, ESAT-6, CFP-10, and MPB83 are produced by M. kansasii. Instillation of M. kansasii (4 x 10(8) cfu) into the tonsilar crypts of calves elicited delayed type hypersensitivity as well as in vitro interferon-y and nitrite responses to M. avium and M. bovis purified protein derivative (PPD). While responses by M. kansasii-inoculated calves to M. avium and M. bovis PPD were roughly equivalent, responses by M. bovis-infected calves (4 x 10(4) cfu intratonsilarly) to M. bovis PPD exceeded (P < 0.05) respective responses to M. avium PPD. Interferon-y and nitrite responses to rESAT-6:CFP-10 by M. kansasii-inoculated calves exceeded (P < 0.05) corresponding responses by non-inoculated calves beginning 15 and 50 days after inoculation, respectively. Ranking of mean interferon-y and nitrite responses to rESAT-6:CFP-10 by each of the groups generally resulted in M. bovis- > M. kansasii- > non-inoculated cattle. Using a lipoarabinomannan-based enzyme-linked immunosorbent assay (LAM-ELISA), specific serum antibody was detected 50 days after challenge in M. kansasii-inoculated calves but not from animals receiving M. bovis. However, at 124 days after inoculation, antibody responses to LAM by M. bovis-inoculated calves exceeded (P < 0.05) respective responses by M. kansasii-inoculated calves. By multi-antigen print immunoassay, specific antibody responses to MPB83, but not ESAT-6 or CFP-10, were detected in sera from M. kansasii-inoculated calves; however, these responses were notably weak as compared to those elicited by M. bovis infection. These findings indicate that M. kansasii infection of calves elicits specific cellular and humoral responses that may confound interpretation of bovine TB tests using antigens generally considered M. tb complex specific.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov