U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 close



Research
                Research Home
                            National Programs
                            Research Projects
                            Scientific Manuscripts
                            International Engagement
                            Scientific Software/Models
                            Databases and Datasets
                            Office of Scientific Quality Review
            Media
                News & Features
                            Multimedia
                            Briefing Room
                            Down on the Farm
                            Press Room
                            Science in Your Shopping Cart
                            Scientific Discoveries
                            Tellus
                            Under the Microscope
            About ARS
                About ARS Home
                            Staff Directory
                            Labs and Research Centers (Map)
                            Headquarter Offices
                            Organizational Chart
                            Employee Services (REE Employees Only)
                            Office of Outreach, Diversity, and Equal Opportunity
            Work With Us
                Work With Us Home
                            ARS Vacancies at USAJOBS
                            Careers at ARS Information
                            Scientific Collaborations
                            Extramural Agreements
                            1890 Faculty Research Sabbatical ProgramSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Northeast Area Beltsville, Maryland (BHNRC) Beltsville Human Nutrition Research Center Food Components and Health Laboratory Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Novotny, Janetitem
                                            KURILICH, ANNE - DAIRY MANAGEMENTitem
                                            Britz, Stevenitem
                                            Baer, Daviditem
                                            Clevidence, Beverly
  Submitted to: Journal of Lipid Research
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 4/15/2004
                                    Publication  Date: 4/15/2004
                                    Citation: Novotny Dura, J., Kurilich, A.C., Britz, S.J., Baer, D.J., Clevidence, B.A. 2004. Appearance of labeled beta-carotene, lutein, retinol, and phylloquinone in plasma after consumption of isotopically-labeled kale. Journal of Lipid Research 46:1896-903Interpretive Summary: none
                                Technical Abstract: To investigate the bioavailability of nutrients from a green, leafy vegetable, isotopically-labeled kale was produced and used in a human feeding trial.  Kale was chosen because of its high content of both beta-carotene and lutein.   The kale was grown in a sealed chamber in the presence of carbon-13 dioxide (99% enrichment) to produce fully labeled plants.  After harvest, blanched kale (400 g single dose) was ingested by 6 volunteers with 30 g safflower oil.  The kale dose supplied approximately 20 mg of both beta-carotene and lutein and 70 micrograms of phylloquinone.  Serial plasma samples were analyzed for labeled and unlabeled analytes by LC-MS.  Peak concentrations (mean+/-SD) of labeled analytes were found to be as follows: 0.063+/-0.031 micromolar for beta-carotene, 0.077+/-0.029  micromolar for retinol, 0.39+/-0.08 micromolar for lutein, and 1.55+/-0.76 nanomolar for phylloquinone.  The inter-individual variability in area under the plasma analyte concentration-time curves were as follows: 41% for beta-carotene, 37% for lutein, 30% for retinol, 67% for phylloquinone. The peak analyte concentrations represented the following percentages of dose: 0.6% for beta-carotene, 3.6% for lutein, 0.7% of molar beta-carotene dose for retinol, and 0.4% of dose for phylloquinone. The isotopic labeling of kale combined with its use in a human feeding trial provides a unique way to examine the comparative bioavailability of nutrients.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov