U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 close



Research
                Research Home
                            National Programs
                            Research Projects
                            Scientific Manuscripts
                            International Engagement
                            Scientific Software/Models
                            Databases and Datasets
                            Office of Scientific Quality Review
            Media
                News & Features
                            Multimedia
                            Briefing Room
                            Down on the Farm
                            Press Room
                            Science in Your Shopping Cart
                            Scientific Discoveries
                            Tellus
                            Under the Microscope
            About ARS
                About ARS Home
                            Staff Directory
                            Labs and Research Centers (Map)
                            Headquarter Offices
                            Organizational Chart
                            Employee Services (REE Employees Only)
                            Office of Outreach, Diversity, and Equal Opportunity
            Work With Us
                Work With Us Home
                            ARS Vacancies at USAJOBS
                            Careers at ARS Information
                            Scientific Collaborations
                            Extramural Agreements
                            1890 Faculty Research Sabbatical ProgramSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            KORTE, SCOTT - UNIVERSITY OF MISSOURIitem
                                            Carroll, Jeffery

                                                         - *Jeff Carroll*item
                                            CARTER, D. BART - UNIVERSITY OF MISSOURIitem
                                            PRATHER, RANDALL - UNIVERSITY OF MISSOURI
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 4/18/2004
                                    Publication  Date: 10/18/2004
                                    Citation: Korte, S.W., Carroll, J.A., Carter, D., Prather, R.S. 2004. Comparison of the innate immune response in cloned and non-cloned pigs following lipopolysaccharide challenge [abstract]. American Association for Laboratory Animal Science.Interpretive Summary:
                                Technical Abstract: The objective of this study was to compare the innate immune response of cloned pigs to normal pigs following a lipopolysaccharide (LPS) challenge using cortisol, tumor necrosis factor-alpha (TNF-alpha), and interleukin 6 (IL-6) as indicators of the induction of an innate immune response.  Nine 28-day-old clones and eleven 28-day-old normal piglets (controls) from the same genetic background were used.  All animals were weaned at 21 days and individually housed in the same experimental environment until the beginning of the study.  The day prior to the LPS challenge, the animals were anesthetized by inhalation anesthesia and a jugular catheter was non-surgically placed.  On day 28, each animal was challenged with 25 ug/kg body weight LPS intravenously via the jugular catheter.  Blood samples were collected every 30 minutes from 2 hours before the LPS challenge, through 4 hours following the challenge. Serum samples from each time point were evaluated for cortisol, TNF-alpha, and IL-6 concentrations.  Results from 2 hours prior to LPS challenge demonstrated that the cloned animals had lower basal serum cortisol (P<0.002) and TNF-alpha (P<0.0001) concentrations compared to control pigs.  The basal concentration of IL-6 was below the detection limit of the kit in both the cloned pigs and the control pigs.  A time-by-group interaction (P<0.0001) was noted following the LPS challenge for serum cortisol, TNF- alpha, and IL-6, with serum concentrations lower in the cloned animals when compared to their non-cloned counterparts.  The results of this study suggest that cloned piglets have a diminished innate immune response, which may account for the apparent increased susceptibility of cloned pigs to perinatal bacterial infection.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov