# Patent

## Title
Vaccines against tularemia

## Abstract

The disclosure relates generally to an antigenic composition useful for immunization against tularemia. The disclosure is a method for producing a vaccine for preventing tularemia in humans and animals, a new vaccine against tularemia in humans and animals, and a new approach to producing vaccines against tularemia.

## Background
This application is a U.S. National Stage Application filed under 35 U.S.C. 371 and claims priority to International Application No. PCT/US10/33352, filed May 3, 2010, which application claims priority from U.S. Provisional Application Ser. No. 61/175,436, filed May 4, 2009, which are incorporated herein by reference.


STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH
This invention was made with Government support of Grant No. DAMD17-03-1-0052, awarded by the United States Army. The Government has certain rights in this invention.


TECHNICAL FIELD
The invention relates generally to an antigenic composition useful for immunization against tularemia. The invention is a method for producing a vaccine for preventing tularemia in humans and animals, a new vaccine against tularemia in humans and animals, and a new approach to producing vaccines against tularemia.
BACKGROUND
The Gram-negative bacterium Francisella tularensis is the causative agent of the zoonotic disease tularemia. Humans acquire tularemia from contact with infected tissues or materials, insect bites, consumption of contaminated food or water, or inhalation of aerosols. F. tularensis consists of 3 subspecies—tularensis, holarctica and mediasiatica—which differ in their geographic distributions and in their virulence in humans. F. tularensis subspecies tularensis, found almost exclusively in North America is highly virulent for humans. As few as 10 organisms subcutaneously or 25 organisms by inhalation can lead to a severe infection. F. tularensis subspecies holarctica (found in North America and in Europe) and subspecies mediasiatica (found in Asia) are of lower virulence. Because of its high infectivity and capacity to cause severe morbidity and mortality, F. tularensis subspecies tularensis is considered a potential agent of bioterrorism.
A vaccine against F. tularensis was developed a half-century ago, but it has not been approved for general use. This vaccine, called Live Vaccine Strain, or LVS, is an attenuated form of Francisella tularensis subspecies holarctica, a much less virulent subspecies of F. tularensis than the highly virulent subspecies of concern as a bioterrorist agent, F. tularensis subspecies tularensis. The LVS vaccine is poorly characterized, unstable in that different colonial morphology types emerge on culture, and somewhat toxic to humans vaccinated with it. Moreover, it may not protect against the high doses of F. tularensis subspecies tularensis that might be released in an airborne bioterrorism attack.
SUMMARY
The invention provides methods and compositions useful for preventing infection caused by Francisella tularensis, the agent of tularemia, and a potential agent of bioterrorism.
The disclosure describes a novel vaccine and vaccine vector comprised of an unmarked attenuated deletional mutant of the LVS vaccine. The deletion comprises a knockout or disruption in a gene called capB or a polynucleotide encoding capB (SEQ ID NO:19). The LVSΔcapB mutant, like the previously evaluated LVSΔwbtDEF mutant, is highly attenuated compared with LVS. Surprisingly, however, the LVSΔcapB mutant induces protective immunity against F. tularensis intranasal challenge comparable to LVS.
In addition, the disclosure describes recombinant versions of LVSΔcapB expressing F. tularensis immunoprotective proteins. In addition, the disclosure envisions immunization utilizing a homologous or heterologous prime-boost vaccination strategy. The heterologous strategy may include priming with one vector, e.g. LVSΔcapB expressing one or more F. tularensis proteins, and boosting with another vector, e.g. L. monocytogenes expressing one or more F. tularensis proteins. Boosting may also include immunizing with the F. tularensis protein or proteins in adjuvant.
The disclosure provides a live vaccine comprising an F. tularensis lacking a polynucleotide encoding a functional CapB (LVSΔcapB). In one embodiment, the F. tularensis expresses or over-expresses an F. tularensis antigen. In a further embodiment, the LVSΔcapB expresses or overexpresses at least one polypeptide selected from the group consisting of AcpA, Bfr, DnaK, FabD, GroEL, IglA, IglB, IglC, VgrG, KatG, Pld, SodB and any combination thereof. In one embodiment, the LVSΔcapB expresses an immunogenic IglC. In another embodiment, the LVSΔcapB expresses an immunogenic IglA. In yet another embodiment, the LVSΔcapB expresses an immunogenic IglA and IglC. In yet another embodiment, the LVSΔcapB expresses an immunogenic IglA, IglC and VgrG.
The disclosure also provides an immunoprotective composition comprising an attenuated F. tularensis lacking a polynucleotide encoding a functional CapB and expressing or overexpressing an antigen useful for inducing an immunoprotective response against Francisella tularensis, said antigen comprising an extracellular or immunogenic polypeptide of F. tularensis or immunogenic fragment thereof linked to transcriptional promoter and termination signals. In one embodiment, the the F. tularensis polypeptide or fragment thereof is selected from the group consisting of AcpA, Bfr, DnaK, FabD, GroEL, IglA, IglB, IglC, VgrG, KatG, Pld, Tul4, SodB, and any combination thereof. In one embodiment, the polypeptide or fragment is IglA and/or IglC antigen of F. tularensis. In yet another embodiment, the composition further comprising a pharmaceutical diluent.
The disclosure also provides a prime-boost immunization method comprising a first immunoprotective composition comprising a LVSΔcapB and a second immunoprotective composition comprising an attenuated vector expressing AcpA, Bfr, DnaK, FabD, GroEL, IglA, IglB, IglC, VgrG, KatG, Pld, Tul4, SodB polypeptide, immunogenic fragement, and any combination thereof. In one embodiment, the second immunoprotective composition comprises an attenuated vector comprises an adenoviral vector or an attenuated L. monocytogenes. 
The disclosure also provides a method of inducing protective immunity in a subject comprising administering a composition of the disclosure to the subject, the composition comprising a LVSΔcapB and may further comprise a LVSΔcapB expressing an F. tularensis antigen.
The disclosure also provides a method of protecting a susceptible host against an infection of Francisella tularensis (F. tularensis) comprising administering to said host an amount of the immunoprotective composition of the disclosure sufficient to invoke an immunoprotective response in the host.
The disclosure also provides a recombinant attenuated F. tularensis lacking a polynucleotide encoding CapB and comprising a polynucleotide encoding at least one extracellular or immunogenic protein, or fragment thereof, of F. tularensis that induces a protective immunity against F. tularensis. In one embodiment, the extracellular or immunogenic protein or fragment F. tularensis is selected from the group consisting of AcpA, Bfr, DnaK, FabD, GroEL, IglA, IglB, IglC, VgrG, KatG, Pld, Tul4, and SodB and any combination thereof.
The disclosure also provides a method of immunization comprising administering a prime-boost combination to a subject, wherein the prime vaccine comprises an F. tularensis lacking a polynuclotide encoding CapB (LVSΔcapB), and wherein the boost vaccine comprises at least one attenuated vector comprising at least one F. tularensis antigen selected from the group consisting of AcpA, Bfr, DnaK, FabD, GroEL, IglA, IglB, IglC, VgrG, KatG, Pld, Tul4 SodB, an immunogenic fragment of any of the foregoing and a combination thereof, or an immunogenic polypeptide selected from the group consisting of AcpA, Bfr, DnaK, FabD, GroEL, IglA, IglB, IglC, VgrG, KatG, Pld, Tul4, SodB, an immunogenic fragment of any of the foregoing and any combination thereof. In one embodiment, the the prime vaccine comprises a recombinant attenuated F. tularensis lacking a ploynucleotide encoding CapB and comprising a polynucleotide encoding at least one extracellular or immunogenic protein, or fragment thereof, of F. tularensis that induces a protective immunity against F. tularensis. In another embodiment, the prime vaccine comprises an antigen selected from the group consisting of IglA, IglC and a combination thereof and the boost vaccine comprises an antigen selected from the group consisting of IglA, IglC and a combination thereof. In yet another embodiment, the boost vaccine comprises L. monocytogenes or an adenovirus.



BRIEF DESCRIPTION OF THE FIGURES
FIG. 1 shows immunization with LVSΔLPS induces modest protective immunity against F. tularensis i.n. challenge. Groups of eight BALB/c mice were immunized i.d. twice, 4 weeks apart, with 1×108 CFU LVSΔwbtDEF or LVSΔwzy. Mice immunized with saline (Sham) or immunized with LVS served as controls. Six weeks later, the mice were challenged i.n. with 4000 CFU LVS. At 5 days post-challenge, the spleen, liver and lung were removed and assayed for bacterial burden. Symbols represent each animal in a group. CFUs were compared by one-way ANOVA (Prism 5) with bonferroni's post-test. Dashed line, limit of detection. ***, P<0.001 vs. sham-immunized mice. ns, not significant.
FIG. 2 shows that LVSΔcapB expresses the O-antigen. The bacteria lysate of LVS or four different clones of LVSΔcapB were anlalyzed by Western blotting (A) using anti-Ft LPS monoclonal antibody FB11 or by Coomassie blue staining for assessment of protein (B). Panel A shows the characteristic ladder pattern of LPS expressed by both strains. Panel B shows the protein pattern of each sample and that comparable amounts of each sample were loaded.
FIG. 3 shows that the LVSΔcapB mutant is not sensitive to serum killing. Each bacterial strain was incubated with either fresh AB serum (ABS) or heat-inactivated AB serum (HI-ABS) for 10 min and assayed for CFU on chocolate agar. Values represent means±standard errors.
FIG. 4 shows that the LVSΔcapB mutant is attenuated for growth in human macrophages. THP-1 cells were co-infected with either LVSΔcapB carrying a hygromycin-resistance gene (ΔcapB-hyg) and LVS carrying a kanamycin-resistance gene (LVS-kan) (solid circle, left vertical axes) or vise versa i.e. with the same two strains carrying the opposite antibiotic resistance marker (solid square, right vertical axes). At the indicated hours post infection, the cell monolayer was lysed and serial dilutions of the lysate were plated on chocolate agar supplemented with either kan or hyg. CFU were counted and the ratio between the number of LVSΔcapB and LVS was calculated at each time point. The decrease in ratio over time indicates that LVS out-competed the LVSΔcapB strain.
FIG. 5 shows that immunization with LVSΔcapB induces potent protective immunity against F. tularensis i.n. challenge. BALB/c mice (4/group) were immunized i.n. (left panel) or i.d. (right panel) with LVSΔcapB with doses shown at the bottom of the figure. Mice immunized with saline (Sham, 8/group) or immunized with LVS (4/group) served as controls. Four weeks later, the mice were challenged i.n. with 4000 CFU LVS. At 5 days post-challenge, the spleen (upper panels), liver (middle panels) and lung (lower panels) were removed and assayed for bacterial burden. Symbols represent each animal in a group. CFUs were compared by one-way ANOVA (Prism 5) with bonferroni's post test. Dashed line, limit of detection. ***, P<0.001 vs. sham-immunized mice.
FIG. 6 shows construction of LVSΔcapB. LVSΔcapB was constructed by allelic exchange between LVS (top panel) and a plasmid carrying an exchange cassette containing flanking sequences to capB (FTL—1416/FTT0805), including the coding sequences for the C-terminal portion of capA and the full length capC (capA'C) on one side and the non-coding sequence between capB and FTL—1417 and the coding sequence for FTL—1417 (middle panel) on the other side. The resultant LVSΔcapB strain (bottom panel) retains 6 amino acids from the N-terminus and 4 amino acids from the C-terminus of CapB and contains no antibiotic resistance marker (unmarked).
FIG. 7A-B shows LVSΔcapB is more attenuated than parental LVS in mice. Groups of 4 BALB/c mice were immunized i.n. (A) or i.d. (B) with LVS or LVSΔcapB at the indicated doses (CFU) and monitored for survival, weight change, local lesions (after i.d. immunization) and other signs of illness for 3 weeks. Relative weight at each time point was calculated as the percentage of the weight at day 2 post-immunization. Values are mean±SE. (A) the relative weight of surviving mice only is shown; (B) weight graphed only through day 6 post immunization at which time only 1 mouse survived; (C) all the mice in this group died by day 6 post immunization.
FIG. 8A-B shows dissemination and clearance of LVSΔcapB in mice. Groups of 4 BALB/c mice were immunized i.n. with 112 CFU LVS or 1×105 CFU LVSΔcapB or i.d. with 1×105 LVS or 1×106 LVSΔcapB. At the indicated times post-immunization, mice were euthanized and CFU in the spleen, liver, lung and skin at the site of injection (after i.d. immunization) assayed. LVSΔcapB is cleared faster than LVS in spleen, liver, and lung after i.n. immunization and at the site of injection in the skin after i.d. immunization. *, P<0.05; **, P<0.01; ***, P<0.001 by ANOVA.
FIG. 9A-D shows immunization and challenge protocols. Groups of BALB/c mice were immunized i.n. or i.d. with LVSΔcapB or LVS. Mice immunized with PBS (Sham) or LVS served as controls. At the indicated times post immunization, mice were either euthanized for assaying lymphocyte proliferation (LPA) or serum antibody level(A) or challenged with approximately 4000 CFU LVS (>5×LD50) by the i.n. route (B and C) or challenged with 10×LD50 of the Type A F. tularensis SchuS4 strain by the aerosol route (D). For assessment of organ bacterial burden, 4 or 8 mice per group challenged with LVS i.n. were euthanized at 5 days post-challenge and the organs were homogenized and assayed for CFU of LVS (B). For assessment of survival, 8 mice per group challenged with LVS i.n. or SchuS4 by aerosol were monitored for signs of illness and death for three weeks after challenge (C and D).
FIG. 10 shows immunization with LVSΔcapB induces cell-mediated immune responses comparable to those induced by LVS. Groups of 4 mice were immunized with LVS or LVSΔcapB by the i.n. or i.d. route. At 4 weeks post immunization, mice were euthanized and splenic lymphocyte proliferation assayed after incubation of splenocytes with heat-inactivated (HI) LVS for 48 hours. *p<0.05; **p<0.01; and ***p<0.001 by 2-way ANOVA.
FIG. 11A-E shows immunization with LVSΔcapB induces potent antibody responses. The antibody level was calculated as Log10 of the reciprocal of the endpoint dilution of the test serum. Data represent mean±SE. *, p<0.05; ***, p<0.001 by 2-way ANOVA.
FIG. 12A-D shows immunization with LVSΔcapB induces protective immunity against i.n. LVS challenge comparable to that of the parental LVS. Groups of 12 mice were sham-immunized, immunized i.d. with 1×105 CFU LVS or 1×106 CFU LVSΔcapB, or immunized i.n. with 200 CFU LVS or 1×105 CFU LVSΔcapB. Four weeks (A and C) or eight weeks (B and D) later, mice were challenged i.n. with 4000 CFU LVS. A and B. At 5 days post-challenge, 4 mice per group were euthanized and the spleen, liver and lung assayed for bacterial burden. CFU values are shown as mean±SE. Dashed line, limit of detection. **, P<0.01 and ***, P<0.001 vs. sham-immunized mice by one-way ANOVA with bonferroni's post test (Prism 5). C and D. The remaining mice in each group were monitored for survival and signs of illness for 3 weeks. The difference in survival between the mice in the vaccinated groups and mice in the sham-vaccinated group were evaluated using a log-rank (Mantel-Cox) test (Prism 5.01). ***, P<0.0001 vs. Sham by log-rank (Mantel-Cox) test. a, 3 of 12 mice (25%) died after i.n. immunization with LVS; of the remaining 9 mice, 4 were studied in Panel A and 5 in Panel C.
FIG. 13A-B shows immunizations with LVSΔcapB rLVSΔcapB/IglA and rLVSΔcapB/IglC induce protective immunity against F. tularensis SchuS4 aerosol challenge. Groups of 8 mice were sham-immunized, immunized i.d. (A) with 1×105 CFU LVS or 1×106 CFU LVSΔcapB, rLVSΔcapB/IglA, or rLVSΔcapB/IglC or immunized i.n. (B and D) with 200 CFU LVS or 1×105 CFU LVSΔcapB, rLVSΔcapB/IglA or rLVSΔcapB/IglC. Six weeks later, all mice were challenged by the aerosol route with 10×LD50 of the F. tularensis SchuS4 strain. Mice were monitored for survival for 3 weeks. Mean survival time was calculated by dividing the sum of the survival times of all mice in a group by the total number of mice challenged, with animals surviving until the end of the experiment given a survival time of 21 days, when the experiment was terminated. The difference in survival between the mice in the vaccinated groups and mice in the sham-vaccinated group were evaluated using a log-rank (Mantel-Cox) test (Prism 5).
FIG. 14A-B shows protective immunity against i.n. challenge with F. tularensis 4 weeks after immunization with parental LVSΔcapB or rLVSΔcapB expressing F. tularensis proteins. BALB/c mice (4/group) were sham-immunized, or immunized i.n. (A) or i.d. (B) with LVS (200 CFU i.n. or 1×105 CFU i.d.), or with LVSΔcapB or LVSΔcapB expressing F. tularensis proteins (1×105 CFU i.n. or 1×106 CFU i.d.). Four weeks later, the mice were challenged i.n. with 4000 CFU LVS. At 5 days post-challenge, the lung, spleen and liver were removed and assayed for bacterial burden. Values for each group are the mean±SE.
FIG. 15A-B shows protective immunity against i.n. challenge with F. tularensis 4 weeks after immunization with parental LVSΔcapB or rLVSΔcapB expressing F. tularensis IglA or IglC. BALB/c mice (4/group) were sham-immunized, or immunized i.n. (A) or i.d. (B) with LVS (200 CFU i.n. or 1×105 CFU i.d.), or with LVSΔcapB, LVSΔcapB/Pgro-IglA, or LVSΔcapB/Pgro-IglC (1×105 CFU i.n. or 1×106 CFU i.d.). Four weeks later, the mice were challenged i.n. with 4000 CFU LVS. At 5 days post-challenge, the lung, spleen and liver were removed and assayed for bacterial burden. Values for each group are the mean±SE.
FIG. 16A-B shows protective immunity against i.n. challenge with F. tularensis 8 weeks after immunization with parental LVSΔcapB or rLVSΔcapB expressing F. tularensis IglA or IglC, as assessed by assay of weight loss after challenge. BALB/c mice (4/group) were sham-immunized, or immunized i.n. (A) or i.d. (B) with LVS (200 CFU i.n. or 1×105 CFU i.d.), or with LVSΔcapB, LVSΔcapB/Pgro-IglA, or LVSΔcapB/Pgro-IglC (1×105 CFU i.n. or 1×106 CFU i.d.). Four weeks later, the mice were challenged i.n. with 4000 CFU LVS and monitored for relative weight change. Values for each group are the mean±SE of the weight at each time point as a percent of the weight at day 0 post challenge.
FIG. 17A-B shows protective immunity against i.n. challenge with F. tularensis 8 weeks after immunization with parental LVSΔcapB or rLVSΔcapB expressing F. tularensis IglA or IglC, as assessed by assay of organ burden after challenge. BALB/c mice (4/group) were sham-immunized, or immunized i.n. (A) or i.d. (B) with LVS (200 CFU i.n. or 1×105 CFU i.d.), or with LVSΔcapB, LVSΔcapB/Pgro-IglA, or LVSΔcapB/Pgro-IglC (1×105 CFU i.n. or 1×106 CFU i.d.). Eight weeks later, the mice were challenged i.n. with 4000 CFU LVS. At 5 days post-challenge, the lung, spleen, and liver were removed and assayed for bacterial burden. Values in each group are the mean±SE.
FIG. 18A-B shows safety of LVSΔcapB or rLVSΔcapB expressing F. tularensis IglA or IglC as assessed by weight loss after immunization. BALB/c mice were sham-immunized, or immunized i.d. (A, 24 mice/group) or i.n. (B, 8 mice/group) with LVS, LVSΔcapB, LVSΔcapB/Pgro-IglA, or LVSΔcapB/Pgro-IglC at doses (CFU) shown in parentheses to the right of the name of each vaccine. The immunized mice were monitored for signs of illness and weighed at the indicated times after immunization. Values in each group are the mean±SE. In Panel A, *, P<0.05, LVS versus the following: Sham, LVSΔcapB, LVSΔcapB/Pgro-IglA or LVSΔcapB/Pgro-IglC, by one-tailed t-test. In Panel B, ***, P<0.001, LVS versus each of the following at days 6, 8, and 10: Sham, LVSΔcapB, LVSΔcapB/Pgro-IglA or LVSΔcapB/Pgro-IglC by two-way ANOVA (GraphPad Prism 5) with Bonferroni post-tests. #, 1 mouse immunized i.n. with LVS died at the indicated time points; thus the result for LVS at day 10 (Panel B) is for the remaining 6 animals in the group.
FIG. 19A-C shows that a heterologous prime-boost vaccination strategy using intradermally administered LVSΔcapB or rLVSΔcapB/Pgro-IglC as the prime and a recombinant attenuated Listeria monocytogenes vaccine expressing IglC as the boost induces strong protective immunity against aerosol challenge with the highly virulent F. tularensis SchuS4 strain. (A, B) Survival. Percent survival was computed at each time point. The difference in survival between the mice in each of the vaccinated groups and mice in the sham-vaccinated group was evaluated using a log-rank (Mantel-Cox) test (Prism 5). Similarly, the difference in survival between the mice in the LVS-vaccinated group and mice in each of the other groups was evaluated using a log-rank (Mantel-Cox) test (Prism 5). ns, not statistically significant. (C) Weight change. Values represent mean±SE from groups of 8 mice.



DETAILED DESCRIPTION
As used herein and in the appended claims, the singular forms “a,” “and,” and “the” include plural referents unless the context clearly dictates otherwise. Thus, for example, reference to “a polynucleotide” includes a plurality of such polynucleotides and reference to “the peptide” includes reference to one or more peptides, and so forth.
Also, the use of “or” means “and/or” unless stated otherwise. Similarly, “comprise,” “comprises,” “comprising” “include,” “includes,” and “including” are interchangeable and not intended to be limiting.
It is to be further understood that where descriptions of various embodiments use the term “comprising,” those skilled in the art would understand that in some specific instances, an embodiment can be alternatively described using language “consisting essentially of” or “consisting of.”
By “about” is meant a quantity, level, value, number, frequency, percentage, dimension, size, amount, weight or length that varies by as much as 30, 25, 20, 25, 10, 9, 8, 7, 6, 5, 4, 3, 2 or 1% to a reference quantity, level, value, number, frequency, percentage, dimension, size, amount, weight or length.
With respect to ranges of values, the invention encompasses each intervening value between the upper and lower limits of the range to at least a tenth of the lower limit's unit, unless the context clearly indicates otherwise. Further, the invention encompasses any other stated intervening values. Moreover, the invention also encompasses ranges excluding either or both of the upper and lower limits of the range, unless specifically excluded from the stated range.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood to one of ordinary skill in the art to which this disclosure belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice of the disclosed methods and compositions, the exemplary methods, devices and materials are described herein.
Any publications discussed above and throughout the text are provided solely for their disclosure prior to the filing date of the present application. Nothing herein is to be construed as an admission that the inventors are not entitled to antedate such disclosure by virtue of prior disclosure.
F. tularensis is a Category A bioterrorism agent that has previously been stock-piled as a germ-warfare agent and may have been used as such in World War II. Especially when spread by the air-borne route, F. tularensis can cause a highly fatal pneumonia.
Francisella tularensis is a nonmotile, nonsporulating, gram-negative coccobacillus that causes zoonotic disease in small animals such as rodents, rabbits, and beavers. Humans acquire tularemia by handling infected animals, by consumption of contaminated food or water, or by the bite of blood-sucking insects. F. tularensis consists of three main subspecies—tularensis, holarctica, and mediasiatica—which differ in their geographic distributions and in their virulence in humans. F. tularensis subspecies tularensis, found almost exclusively in North America, is highly virulent for humans. As few as 10 organisms delivered subcutaneously or 25 organisms delivered by inhalation can lead to a severe, potentially lethal, infection in humans. F. tularensis subspecies holarctica (found in North America and in Europe) and subspecies mediasiatica (found in Asia) are of lower virulence. Because of its high infectivity and capacity to cause severe morbidity and mortality, F. tularensis subspecies tularensis is classified as a category A potential agent of bioterrorism.
Although F. tularensis can be grown in the laboratory on enriched culture media, F. tularensis bacteria invade and grow productively in macrophages. It is thought that, in natural infections, the bacterium replicates intracellularly within host mononuclear phagocytes. After entry of the organism into the macrophages, F. tularensis initially resides in a phagosome. However, the bacterium arrests the maturation of its phagosome, which acquires some markers of early and late endosomes, but not cathepsin D, and it inhibits the acidification of its phagosome. A unique feature of the phagosome is that it is often surrounded by a dense fibrillar coat. With more time after infection, the phagosomal membrane is disrupted and the bacterium replicates freely in the cytoplasm of the macrophage. While these aspects of intracellular life after entry have been reported, the ultrastructure and mechanisms that mediate uptake of this highly infectious bacterium have not previously been reported.
The genomic sequences of certain strains of F. tularensis are known as set forth below (the sequences associated with these accession numbers are incorporated herein by reference): NC—010677, Francisella tularensis subsp. mediasiatica FSC147, complete genome DNA, circular, Length: 1,893,886 nt, Replicon Type: chromosome; NC—009749, Francisella tularensis subsp. holarctica FTNF002-00, complete genome, DNA, circular, Length: 1,890,909 nt, Replicon Type: chromosome; NC—008601, Francisella tularensis subsp. novicida U112, complete genome, DNA, circular, Length: 1,910,031 nt, Replicon Type: chromosome; NC—009257, Francisella tularensis subsp. tularensis WY96-3418, complete genome, DNA, circular, Length: 1,898,476 nt, Replicon Type: chromosome; NC—007880, Francisella tularensis subsp. holarctica LVS, complete genome, DNA, circular, Length: 1,895,994 nt, Replicon Type: chromosome. NC—006570, Francisella tularensis subsp. tularensis Schu 4, complete genome, DNA, circular, length: 1,892,819 nt, Replication Type: chromosome. NC—008245, Francisella tularensis subsp. tularensis FSC 198, complete genome, DNA, circular, length: 1,892,616 nt, Replication Type: chromosome.
The disclosure provides an attenuated recombinant F. tularensis useful as a vaccine or for the delivery of antigens for vaccine purposes.
A brief description of the immune system will assist in understanding the disclosure. There are two arms to the immune response: a humoral (antibody) response and a cell-mediated response. Protein antigens derived from pathogens that replicate intracellularly (viruses and some bacteria) are processed within the infected host cell releasing short peptides which are subsequently displayed on the infected cell surface in association with class I major histocompatability (MHC I) molecules. When this associated complex of MHC I and peptide is contacted by antigen-specific CD8+ T-cells the T-cell is activated, acquiring cytotoxic activity. These cytotoxic T-cells (CTLs) can lyse infected host cells, so limiting the replication and spread of the infecting pathogen. Another important arm of the immune response is controlled by CD4+ T-cells. When antigen derived from pathogens is released into the extracellular milieu they may be taken up by specialised antigen-presenting cells (APCs) and displayed upon the surface of these cells in association with MHC II molecules. Recognition of antigen in this complex stimulates CD4+ T-cells to secrete soluble factors (cytokines) which regulate the effector mechanisms of other T-cells. Antibody is produced by B-cells. Binding of antigen to secreted antibody may neutralise the infectivity of a pathogen and binding of antigen to membrane-bound antibody on the surface of B-cells stimulates division of the B-cell so amplifying the B-cell response. In general, good antibody responses are required to control bacterial infections and both antibody and cell-mediated immune responses (CD8+ and CD4+) are required to control infections by viruses.
“CD8+ T cells” represent a class of T lymphocytes characterized by the possession of the CD8 cell surface marker. CD8+ T cells are MHC Class I-restricted “CTLs” or “suppressor T cells.”
“CD4+ T cells” represent a class of T lymphocytes characterized by the possession of the CD4 cell surface marker. CD4+ T cells are MHC Class 11-restricted T lymphocytes. There are two types of CD4+ T cells referred to as type 1 or type 2 “helper T cells.”
The driving force behind the development of these two types of immune responses is cytokines, a number of identified protein messengers which serve to help the cells of the immune system and steer the eventual immune response to either a Th1 or Th2 response. Thus, high levels of Th1-type cytokines tend to favor the induction of cell mediated immune responses to the given antigen, while high levels of Th2-type cytokines tend to favor the induction of humoral immune responses to the antigen. It is important to remember that the distinction of Th1 and Th2-type immune responses is not absolute. In reality, an individual will support an immune response which is described as being predominantly Th1 or predominantly Th2. Traditionally, Th1-type responses are associated with the production of the INF-γ and IL-2 cytokines by T-lymphocytes. Other cytokines often directly associated with the induction of Th1-type immune responses are not produced by T-cells, such as IL-12. In contrast, Th2-type responses are associated with the secretion of IL-4, IL-5, IL-6, IL-10 and tumour necrosis factor-β (TNF-β).
The disclosure comprises several types of vaccines. One vaccine is LVSΔcapB. Other vaccines comprise LVSΔcapB expressing one or more F. tularensis antigens, e.g., IglC, IglA, IglB, and/or VgrG (PigB) as described below.
A vaccine of the disclosure can be administered intranasally, intradermally or by another route, e.g. subcutaneously, intramuscularly, inhaled, or even orally to a mammalian host. The vaccine can be administered as part of a homologous or heterologous prime-boost strategy.
The vaccine induces a strong cell-mediated immune response to pathogen antigens in the vaccine. Most importantly, the vaccine protects the mammalian hosts against infection with F. tularensis. 
In one embodiment, the vaccine is administered to humans or animals by inhalation including intranasally, injection intradermally or by another route, e.g. subcutaneously, intramuscularly, or orally.
As demonstrated herein, the vaccines of the disclosure are safer than prior vaccines. Remarkably, despite their attenuation, their efficacy is comparable and exceeds LVS vaccines.
The disclosure provides a live recombinant F. tularensis (e.g., an attenuated F. tularensis) lacking a polynucleotide encoding a CapB polypeptide (SEQ ID NO:20) or homologs or variants thereof (e.g., having at least 90-99% identity to SEQ ID NO:20), referred to herein as LVSΔcapB. The LVSΔcapB is recombinantly produced by knocking out or disrupting a polynucleotide encoding the CapB polypeptide.
The term “attenuated,” when used with respect to a bacterium, means that the bacterium has lost some or all of its ability to proliferate and/or cause disease or other adverse effect when the bacterium infects an organism. For example, an “attenuated” bacterium can be unable to replicate at all, or be limited to one or a few rounds of replication, when present in an organism in which a wild-type or other pathogenic version of the attenuated bacteria can replicate. Alternatively or additionally, an “attenuated” bacterium might have one or more mutations in a gene or genes (e.g., encoding a functional O-antigen) that are involved in pathogenicity of the bacteria. Many genes, loci, or operons are known, mutations in which will result in an attenuated bacterium.
A “polynucleotide” generally refers to any polyribonucleotide (RNA) or polydeoxribonucleotide (DNA), which may be unmodified or modified RNA or DNA. Polynucleotides include, without limitation, single-stranded and double-stranded DNA, DNA that is a mixture of single-stranded and double-stranded regions, single-stranded and double-stranded RNA, and RNA that is a mixture of single-stranded and double-stranded regions. Polynucleotides also include hybrid molecules comprising DNA and RNA that may be single-stranded or, more typically, double-stranded or a mixture of single-stranded and double-stranded regions. In addition, “polynucleotide” refers to triple-stranded regions comprising RNA or DNA or both RNA and DNA. Polynucleotides also include DNAs or RNAs containing one or more modified bases and DNAs or RNAs with backbones modified for stability or for other reasons. “Modified” bases include, for example, tritylated bases and unusual bases such as inosine. A variety of modifications may be made to DNA and RNA; thus, “polynucleotide” embraces chemically, enzymatically or metabolically modified forms of polynucleotides as typically found in nature, as well as the chemical forms of DNA and RNA characteristic of viruses and cells. Oligonucleotides are relatively short polynucleotides. Examples of polynucleotides encoding F. tularensis antigens are set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, 17, 21, and 23 (or fragments thereof encoding antigenic epitopes).
A “polypeptide” refers to any polypeptide comprising two or more amino acids joined to each other by peptide bonds or modified peptide bonds. “Polypeptide” refers to both short chains, commonly referred to as peptides, oligopeptides or oligomers, and to longer chains, generally referred to as proteins. Polypeptides may contain amino acids other than those normally encoded by a codon.
Polypeptides include amino acid sequences modified either by natural processes, such as post-translational processing, or by chemical modification techniques that are well known in the art. Such modifications are well described in the literature and are known in the art. Modifications may occur anywhere in a polypeptide, including the peptide backbone, the amino acid side-chains and the amino or carboxyl termini. Such modifications may be present to the same or varying degrees at several sites in a given polypeptide. Also, a given polypeptide may contain many types of modifications. Polypeptides may be branched as a result of ubiquitination, and they may be cyclic, with or without branching. Cyclic, branched and branched cyclic polypeptides may result from post-translation natural processes or may be made by synthetic methods. Modifications include acetylation, acylation, ADP-ribosylation, amidation, biotinylation, covalent attachment of flavin, covalent attachment of a heme moiety, covalent attachment of a nucleotide or nucleotide derivative, covalent attachment of a lipid or lipid derivative, covalent attachment of phosphotidylinositol, cross-linking, cyclization, disulfide bond formation, demethylation, formation of covalent cross-links, formation of cystine, formation of pyroglutamate, formylation, gamma-carboxylation, glycosylation, GPI anchor formation, hydroxylation, iodination, methylation, myristoylation, oxidation, proteolytic processing, phosphorylation, prenylation, racemization, selenoylation, sulfation, transfer-RNA mediated addition of amino acids to proteins such as arginylation, and ubiquitination. Examples of polypeptides useful in the methods and compositions of the invention comprise the F. tularensis polypeptide comprising CapB and antigens set forth in SEQ ID Nos: 2, 4, 6, 8, 10, 12, 14, 16, and 18 and antigenic fragments thereof.
A “vaccine” as used herein refers to a composition of matter comprising an attenuated cell or microorganism that induces an immune response, a molecule that, when administered to a subject, induces an immune response, or a combination of an attenuated or live vector that express an antigen that induces an immune response. Vaccines can comprise polynucleotide molecules, polypeptide molecules, and carbohydrate molecules, as well as derivatives and combinations of each, such as glycoproteins, lipoproteins, carbohydrate-protein conjugates, fusions between two or more polypeptides or polynucleotides, and the like. A vaccine may further comprise a diluent, an adjuvant, a carrier, or combinations thereof, as would be readily understood by those in the art.
A vaccine may be comprised of separate components. As used herein, “separate components” refers to a situation wherein the term vaccine actually comprises two discrete vaccines to be administered separately to a subject. In that sense, a vaccine comprised of separate components may be viewed as a kit or a package comprising separate vaccine components. For example, in the context of the disclosure, a package may comprise a first immunogenic composition comprising an attenuated bacterial vector and a second antigenic composition comprising an attenuated viral vector comprising the same or different F. tularensis antigens (e.g., AcpA, Bfr, DnaK, FabD, GroEL, IglA, IglB, IglC, KatG, Pld and SodB).
A vaccine “induces” an immune response when the microorganism, antigen or antigens present in the vaccine cause the vaccinated subject to mount an immune response to that antigen or antigens. The vaccinated subject will generate an immune response, as evidenced by activation of the immune system, which includes the production of vaccine antigen-specific T cells, vaccine antigen-specific B cells, vaccine antigen-specific antibodies, and cytokines. The resulting immune response may be measured by several methods including ELISPOT, ELISA, chromium release assays, intracellular cytokine staining, FACS analysis, and MHC tetramer staining (to identify peptide-specific cells). A skilled artisan may also use these methods to measure a primary immune response or a secondary immune response.
An “antigen” is a substance capable of generating an immune response in a subject exposed to the antigen. Antigens are usually polypeptides and are the focus of the host's immune response. An “epitope” or “antigenic determinant” is that part of an antigen to which T cells and antibodies specifically bind. An antigen may contain multiple epitopes. Antigens of the disclosure comprise F. tularensis extracellular polypeptides. In specific aspect, the F. tularensis polypeptides comprise AcpA, Bfr, DnaK, FabD, GroEL, IglC, KatG, Pld, SodB, IglA and IglB (SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14, 16, 18, 22 and 24, respectively).
A priming vaccine used in the method of the disclosure comprises an F. tularensis antigen (e.g., IglC as the prime vaccine). The priming vaccine may be an antigenic epitope of an F. tularensis antigen, the full length antigen, a vector comprising a polynucleotide encoding the antigen (e.g., a LVSΔcapB/Pgro-IglC) and the like. In one embodiment, the priming vaccine comprises a polynucleotide encoding an antigen under control of a foreign promoter within a bacterium or virus. The polynucleotide of the priming vaccine is present in a suitable delivery vector such as a plasmid or other vector such as a bacterial or viral vector. The polynucleotide may be under the control of a suitable promoter such as a promoter derived from the HCMV IE gene. In specific aspects, the rLVSΔcapB vaccines comprise polynucleotides encoding F. tularensis antigens IglC, IglA, VgrG, and/or IglB under the control of the F. tularensis LVS groE operon promoter. The recombinant Listeria monocytogenes vaccines comprise polynucleotides encoding F. tularensis antigens IglC, IglA, VgrG, and/or IglB under the control of the L. monocytogenes hly or actA promoter. The priming vaccine is administered in an amount effective for priming an immune response to the F. tularensis antigen. As used herein, “priming” of an immune response occurs when an antigen is presented to T cells or B cells. As a result, primed cells can respond to the same antigen again as memory cells in a second, subsequent immune response. Thus, priming generates both the primary immune response and establishes immunological memory. One skilled in this art appreciates that a primary immune response represents the adaptive immune response upon initial exposure to an antigen in a particular context such as in the pathogen or in a vaccine. However, it will also be appreciated that the disclosure is not limited to use of the priming vaccine in the context of immunologically naive individuals. Rather, priming may also occur in individuals who have been exposed to the antigen but who have not received the priming vaccine. In a specific embodiment, the priming vaccine comprises an attenuated F. tularensis vector.
The priming immunogenic (vaccine) composition may be administered once before administration of the boosting immunogenic (vaccine) composition. In another embodiment, the priming vaccine may be administered several times.
The boosting vaccine used in the method of the disclosure may comprise at least one F. tularensis antigen polypeptide. The boosting vaccine may comprise the same or a different antigen. The boosting vaccine may comprise the same or a different vector. In one embodiment, the boosting vaccine comprises an additional F. tularensis polypeptide antigen to enhance the immunogenicity of of the subject to F. tularensis. For example in one embodiment, the boosting vaccine comprises an F. tularensis antigen expressed in a viral vector. The F. tularensis antigen can be selected from the group consisting of AcpA, Bfr, DnaK, FabD, GroEL, IglC, KatG, Pld, SodB, IglA, IglB, and VgrG. In a specific embodiment, the boosting vaccine comprises IglC and/or KatG expressed in an adenovirus vector. In a specific embodiment, a vaccine combination of the disclosure comprises a prime vaccine of LVSΔcapB F. tularensis expressing an antigen from F. tularensis and a boosting vaccine of adenovirus expressing the same or a different F. tularensis antigen. In another specific embodiment, a vaccine combination of the disclosure comprises a prime vaccine of LVSΔcapB F. tularensis and a boosting vaccine of attenuated L. monocytogenes expressing an F. tularensis antigen. In another specific embodiment, a vaccine combination of the disclosure comprises a prime vaccine of LVSΔcapB F. tularensis expressing an antigen from F. tularensis and a boosting vaccine of attenuated L. monocytogenes expressing the same or a different F. tularensis antigen.
The boosting vaccine is administered in an amount effective for “boosting” a primed immune response to the F. tularensis antigen. As used herein, “boosting” an immune response means to induce a secondary immune response in a subject that has been primed (i.e., already exposed) by an initial exposure to an antigen. A secondary immune response is characterized by the activation and expansion of specific memory T cells and B cells. Thus, boosting a specific immune response augments the primed immune response by inducing immune cells to proliferate and differentiate upon subsequent exposure to that antigen. The boosting vaccine may achieve one or more of the following effects: induces CD4+ T cells, induces anti-F. tularensis antibodies, boosts the activity of the CD8+ T cells primed by the priming vaccine, and induces additional CD8+ T cells not originally identified in the initially primed immune response. The boosting vaccine may also induce CD4+ T cells and induce anti-F. tularensis antibodies.
Certain vaccine adjuvants are particularly suited to the stimulation of either Th1 or Th2-type cytokine responses. Traditionally, the best indicators of the Th1:Th2 balance of the immune response after a vaccination or infection includes direct measurement of the production of Th1 or Th2 cytokines by T lymphocytes in vitro after restimulation with antigen, and/or the measurement of the IgG1:IgG2a ratio of antigen specific antibody responses. Thus, a Th1-type adjuvant is one which stimulates isolated T-cell populations to produce high levels of Th1-type cytokines when re-stimulated with antigen in vitro, and induces antigen specific immunoglobulin responses associated with Th1-type isotype.
As noted herein, the vaccines of the disclosure comprising an attenuated F. tularensis (e.g., a LVSΔcapB) are safer, more stable, and more efficacious than the previously developed but never approved LVS vaccine.
Attenuated vaccines can be administered directly to the mammal. The immunogenic compositions and vaccines obtained using the methods of the disclosure can be formulated as pharmaceutical compositions for administration in any suitable manner. One route of administration is oral. Other routes of administration include rectal, intrathecal, buccal (e.g., sublingual) inhalation, intranasal, and transdermal and the like (see e.g. U.S. Pat. No. 6,126,938). Although more than one route can be used to administer a particular composition, a particular route can often provide a more immediate and more effective reaction than another route.
The disclosure provides an immunogenic composition and vaccine that utilize a live attenuated recombinant F. tularensis vector to deliver F. tularensis immunogenic antigens. The rationale for using live attenuated F. tularensis subspecies holarctica (LVS or LVSΔcapB) as a vector was that this bacterium would deliver antigens in host cells in a way that mimicked a wild-type F. tularensis. Like F. tularensis subspecies tularensis, LVS is an intracellular bacterium that resides in host mononuclear phagocytes. F. tularensis LVS escapes the phagosome and resides in the cytoplasm of the host cell. Thus, F. tularensis LVS releases potentially immunoprotective antigens into the host cell cytoplasm, after which they are processed and presented to the immune system. Such antigens are presented to the immune system via MHC class I molecules, resulting in the priming of CD8 T-cells. When F. tularensis is utilized as a vector for the release of F. tularensis immunogenic antigens, the antigens are processed and presented in a way that mimics their processing and presentation by natural infection and thereby stimulate a highly potent immunoprotective response.
In addition, the disclosure envisions immunization utilizing a homologous or heterologous prime-boost vaccination strategy. The heterologous strategy may include priming with one vector, e.g., an attenutated F. tularensis expressing one or more proteins, and boosting with another vector, e.g., adenovirus or attenuated L. monocytogenes expressing the same protein or proteins, or vice versa. Boosting may also include immunizing with an F. tularensis protein or proteins or fragments thereof in an adjuvant. The specific examples provided herein demonstrate the delivery of the antigens to an animal host utilizing various vaccination strategies and the resulting immunoprotection against F. tularensis challenge.
The disclosure comprises several types of vaccines. One group of vaccines consists of attenuated F. tularensis expressing one or more F. tularensis antigens. Each vaccine is administered intradermally or by another route, e.g. subcutaneously, intramuscularly, intranasally, inhaled, or even orally to a mammalian host. The vaccine can be administered as part of a homologous or heterologous prime-boost strategy. The vaccine induces a strong cell-mediated immune response to pathogen antigens in the vaccine. Most importantly, the vaccine protects the mammalian hosts against infection with F. tularensis. 
The antigens can be delivered via an attenuated vector comprising a polynucleotide encoding an antigenic polypeptide of F. tularensis that results in presentation of the encoded polypeptide via MHC class I or MHC class II. Examples of attenuated vectors useful in the disclosure for combination prime-boost therapies include L. monocytogenes, F. tularensis lacking a functional O-Antigen, F. tularensis Live Vaccine Strain (LVS), or another attenuated bacterial vector such as Mycobacterium bovis BCG, Shigela flexneri and Escherichia coli. Examples of attenuated bacteria used as live vaccines include S. typhi carrying a mutation in its galE or htrA gene, and V. cholerae carrying mutations in its ctxA gene. For example, the recombinant Listeria monocytogenes strains expressing F. tularensis IglC and KatG (rLM/IglC or rLM/KatG) were demonstrated to protect against aerosol challenge with the highly virulent Type A F. tularensis subspecies tularensis SchuS4 Strain.
Microorganisms which are used to express an F. tularensis antigen for use in immunoprotective compositions include, without limitation, Campylobacter sp., Yersinia sp., Helicobacter sp., Gastrospirillum sp., Bacteroides sp., Klebsiella sp., Lactobacillis sp., Streptococcus gordonii, Enterobacter sp., Salmonella sp., Shigella sp., Aeromonas sp., Vibrio sp., Clostridium sp., Enterococcus sp. and Escherichia coli (see e.g. U.S. Pat. Nos. 5,858,352, and 6,051,416, and Levine et al., in “New Generation Vaccines Second Edition” ed. Levine et al., Marcel Dekker, Inc. pp 351-361 (1997), Levine et al., in “New Generation Vaccines Second Edition” ed. Levine et al., Marcel Dekker, Inc. pp 437-446 (1997), Butterton et al., in “New Generation Vaccines Second Edition” ed. Levine et al., Marcel Dekker, Inc. pp 379-385 (1997) and Fennelly et al., in “New Generation Vaccines Second Edition” ed. Levine et al., Marcel Dekker, Inc. pp 363-377 (1997)). For example, Campylobacter jejuni, Campylobacter coli, Listeria monocytogenes, Yersinia enterocolitica, Yersinia pestis, Yersinia pseudotuberculosis, Escherichia coli, Shigella flexneri, Shigella sonnei, Shigella dysenteriae, Shigella boydii, Helicobacter pylori, Helicobacter felis, Gastrospirillum hominus, Vibrio cholerae, Vibrio parahaemolyticus, Vibrio vulnificus, Bacteroides fragilis, Clostridium difficile, Salmonella typhimurium, Salmonella typhi, Salmonella gallinarum, Salmonella pullorum, Salmonella choleraesuis, Salmonella enteritidis, Klebsiella pneumoniae, Enterobacter cloacae, and Enterococcus faecalis. Escherichia coli include but are not limited to entero-toxic, entero-hemorrhagic, entero-invasive, entero-pathogenic or other strains can be used in the disclosure.
The disclosure also provides an immunogenic composition and vaccine that uses an antigen delivery method that deliveries F. tularensis immunogenic antigens in a manner that is similar to delivery in a natural infection. F. tularensis antigens are delivered in one or more vectors capable of inducing presentation via Major Histocompatability Complex (MHC) I.
In one aspect, the disclosure provides an immunogenic composition and vaccine that utilize a live attenuated recombinant Listeria monocytogenes vector to deliver F. tularensis immunogenic antigens. A rationale for using live attenuated L. monocytogenes as a vector is the similarity in the infective process between L. monocytogenes and F. tularensis (other attenuated bacterial vectors having a similar infective process can be used). Like F. tularensis, L. monocytogenes is an intracellular bacterium that resides in host mononuclear phagocytes. Importantly, L. monocytogenes escapes the phagosome in which it initially resides and subsequently inhabits the cytoplasm of the host cell. F. tularensis also escapes the phagosome and resides in the cytoplasm of the host cell. Thus, F. tularensis releases potentially immunoprotective antigens into the host cell cytoplasm, after which they are processed and presented to the immune system. Such antigens are presented to the immune system via MHC class I molecules, resulting in the priming of CD8 T-cells. When L. monocytogenes is utilized as a vector for the release of F. tularensis immunogenic antigens, the antigens are processed and presented in a way that mimics their processing and presentation by F. tularensis and thereby stimulate an immunoprotective response (see, e.g., PCT/US07/22418, filed Oct. 22, 2007, incorporated herein by reference for all purposes). In another aspect, an attenuated F. tularensis lacking a wild-type O-antigen is used. In another aspect, the attenuated F. tularensis LVS strain is used.
Alternatively, or in addition to, a non-bacterial attenuated vector such as a replication-deficient viral vectors may be used in the methods and compositions of the disclosure. Such viral vectors useful in the methods and compositions of the invention include, but are not limited to, Vaccinia, Avipox, Adenovirus, AAV, Vaccinia virus NYVAC, Modified vaccinia strain Ankara (MVA), Semliki Forest virus, Venezuelan equine encephalitis virus, and herpes viruses. Naked DNA vectors can also be used in addition to antigenic proteins alone or in combination with an adjuvant.
Examples of suitable viral vectors include herpes simplex viral vectors, vaccinia or alpha-virus vectors and retroviruses, including lentiviruses, adenoviruses and adeno-associated viruses. In one embodiment, these vectors are replication defective virus vectors. Gene transfer techniques using these viruses are known to those skilled in the art. Retrovirus vectors, for example, may be used to stably integrate the polynucleotide of the invention into the host genome, although such recombination may not be advisable. Replication-defective adenovirus vectors by contrast remain episomal and therefore allow transient expression.
In a specific embodiment, the adenovirus used as a live vector is a replication defective human or simian adenovirus. Typically these viruses contain an E1 deletion and may be grown on cell lines that are transformed with an E1 gene. Suitable Simian adenoviruses are, for example, viruses isolated from Chimpanzee. Examples of viruses suitable for use in the present invention include C68 (also known as Pan 9) (U.S. Pat. No. 6,083,716, incorporated herein by reference) and Pan 5, 6 and Pan 7 (WO 03/046124 incorporated herein by reference). Thus, these vectors can be manipulated to insert a heterologous polynucleotide coding for an antigen such that the product is expressed. The use formulation and manufacture of such recombinat adenoviral vectors is set forth in detail in WO 03/046142, which is incorporated by reference.
The disclosure provides an immunogenic composition and vaccine that uses an antigen delivery method that deliveries F. tularensis immunogenic antigens in a manner that is similar to delivery in a natural infection. F. tularensis antigens are delivered in one or more vectors capable of inducing presentation via Major Histocampatability Complex (MHC) I.
Exemplary prime-boost therapies are set forth in Table 1 (rLM=recombinant L. monocytogenes; rAdv=recombinant adenovirus):








TABLE 1





Prime
Boost







LVSΔcapB
rLM/IglC and/or rLM/KatG and/or rLM/Tul4



and/or rLM/AcpA and/or rLM/Bfr and/or



rLM/GroEL and/or rLM/Pld and/or rLM/DnaK



and/or rLM/IglA and or rLM/IglB


LVSΔcapB
rAdv/IglC and/or rAdv/KatG or rAdv/IglC-



KatG or rAdv/KatG-IglC


LVSΔcapB/IglC
rLM/IglC


LVSΔcapB/IglA
rLM/IglA


LVSΔcapB/IglB
rLM/IglB


LVSΔcapB/AcpA
rLM/AcpA


LVSΔcapB/Bfr
rLM/Bfr


LVSΔcapB/DnaK
rLM/DnaK


LVSΔcapB/GroEL
rLM/GroEL


LVSΔcapB/IglC
rAdv/IglC


LVSΔcapB/IglC
The purified protein(s) expressed by the


and/or
prime vaccine in a suitable carrier with


LVSΔcapB/IglB
a suitable adjuvant


and or


LVSΔcapB/KatG


and/or


LVSΔcapB/Tul4


and/or


LVSΔcapB/AcpA


and/or LVSΔcapB/Bfr


and/or


LVSΔcapB/GroEL


and/or LVSΔcapB/Pld


and/or


LVSΔcapB/DnaK


LVSΔcapB/IglC
IglC in a suitable carrier with a



suitable adjuvant


LVSΔcapB/IglA
IglA in a suitable carrier with a



suitable adjuvant


LVSΔcapB/IglB
IglB in a suitable carrier with a



suitable adjuvant


LVSΔcapB/AcpA
AcpA and/or Bfr and/or DnaK and/or GroEL


and/or LVSΔcapB/Bfr
a suitable carrier with a suitable


and/or
adjuvant


LVSΔcapB/DnaK


and/or


LVSΔcapB/GroEL









The immunoprotective compositions to be administered are provided in a pharmaceutically acceptable solution such as an aqueous solution, often a saline or buffered solution, or they can be provided in powder form. There is a wide variety of suitable formulations of pharmaceutical compositions of the invention. See, e.g., Lieberman, Pharmaceutical Dosage Forms, Marcel Dekker, Vols. 1-3 (1998); Remington's Pharmaceutical Science, 17th ed., Mack Publishing Company, Easton, Pa. (1985) and similar publications. The compositions may also include an adjuvant. Examples of known suitable adjuvants include alum, aluminum phosphate, aluminum hydroxide, and MF59 (4.3% w/v squalene, 0.5% w/v Tween 80, 0.5% w/v Span 85)—these are the only ones currently licensed for use in humans. For experimental animals, one can use Freund's, N-acetyl-muramyl-L-threonyl-D-isoglutamine (thr-MDP), N-acetyl-nor-muramyl-L-alanyl-D-isoglutamine (CGP 11637, referred to as nor-MDP), N-acetylmuramyl-L-alanyl-D-isoglutaminyl-L-alanine-2-(1′-2′-dip-almitoyl-sn-glycero-3-hydroxyphosphoryloxy)-ethylamine (CGP 19835A, referred to as MTP-PE), and RIBI, which contains three components extracted from bacteria, monophosphoryl lipid A, trehalose dimycolate and cell wall skeleton (MPL+TDM+CWS) in a 2% squalene/Tween 80 emulsion, or Bacille Calmette-Guerin (BCG). The effectiveness of an adjuvant may be determined by measuring the amount of antibodies directed against the immunogenic antigen.
The number of LVSΔcapB microorganisms (with or without an expressed antigenic peptide or polypeptide) and the concentration of immunogenic antigens of the disclosure in the pharmaceutical formulations can vary widely, e.g., from less than about 0.1%, usually at or at least about 2% to as much as 20% to 50% or more by weight, and will be selected primarily by fluid volumes, viscosities, and the like, in accordance with the particular mode of administration selected.
Formulations suitable for oral administration can comprise (a) liquid solutions, such as an effective amount of the recombinant bacteria suspended in diluents, such as buffered water, saline or PEG 400; (b) capsules, sachets or tablets, each containing a predetermined amount of the active ingredient, as lyophilized powder, liquids, solids, granules or gelatin; (c) suspensions in an appropriate liquid; and (d) suitable emulsions. Tablet forms can include one or more of lactose, sucrose, mannitol, sorbitol, calcium phosphates, corn starch, potato starch, tragacanth, microcrystalline cellulose, acacia, gelatin, colloidal silicon dioxide, croscarmellose sodium, talc, magnesium stearate, stearic acid, and other excipients, colorants, fillers, binders, diluents, buffering agents, moistening agents, preservatives, flavoring agents, dyes, disintegrating agents, and pharmaceutically compatible carriers. Lozenge forms can comprise the active ingredient in a flavor, usually sucrose and acacia or tragacanth, as well as pastilles comprising the active ingredient in an inert base, such as gelatin and glycerin or sucrose and acacia emulsions, gels, and the like containing, in addition to the active ingredient, carriers known in the art. It is recognized that the attenuated vaccines, when administered orally, must be protected from digestion. This is typically accomplished either by complexing the vaccines with a composition to render it resistant to acidic and enzymatic hydrolysis or by packaging the vaccines in an appropriately resistant carrier such as a liposome or enteric coated capsules. Means of protecting the attenuated bacteria from digestion are well known in the art. The pharmaceutical compositions can be encapsulated, e.g., in liposomes, or in a formulation that provides for slow release of the active ingredient.
The attenuated vaccines, alone or in combination with other suitable components, can be made into aerosol formulations (e.g., they can be “nebulized”) to be administered via inhalation. Aerosol formulations can be placed into pressurized acceptable propellants, such as dichlorodifluoromethane, propane, nitrogen, and the like.
The dose administered to a subject, in the context of the invention should be sufficient to effect a beneficial therapeutic and/or prophylactic response in the subject over time. The dose will be determined by the efficacy of the particular attenuated vaccine employed and the condition of the subject, as well as the body weight or vascular surface area of the subject to be treated. The size of the dose also will be determined by the existence, nature, and extent of any adverse side-effects that accompany the administration of a particular vaccine in a particular subject.
In determining the effective amount of the vaccine to be administered in the treatment or prophylaxis of an infection or other condition, the physician evaluates vaccine toxicities, progression of the disease, and the production of anti-vaccine vector antibodies, if any.
The compositions are administered to an animal that is at risk from acquiring an infection caused by F. tularensis or to prevent or at least partially arrest the development of the infection and its complications. An amount adequate to accomplish this is defined as a “therapeutically effective dose.” Amounts effective for therapeutic use will depend on, e.g., the antigen composition, the manner of administration, the weight and general state of health of the subject, and the judgment of the prescribing physician. Single or multiple doses of the antigen compositions may be administered depending on the dosage and frequency required and tolerated by the subject, and route of administration. In addition, a booster may be administered in the same of different formulation. For example, the method contemplates administration of a first composition comprising an F. tularensis antigen in an attenuated bacterial vector and a second composition comprising an F. tularensis antigen in an attenuated non-bacterial vector. The second composition may be administered simultaneously or subsequent to administration of the first immunogenic composition.
In particular embodiments, a therapeutically effective dose of the immunoprotective composition is administered to a subject. Amounts of live attenutated bacteria or non-bacteria expressing the F. tularensis or other antigens present in the initial immunization generally range from about 1×102 to 1×1011 organisms per subject, and more commonly from about 1×103 to 1×109 organisms per subject.
The existence of an immune response to the first dose of the immunoprotective composition may be determined by known methods (e.g. by obtaining serum from the individual before and after the initial immunization, and demonstrating a change in the individual's immune status, for example an immunoprecipitation assay, or an ELISA, or a bactericidal assay, or a Western blot, or flow cytometric assay, or the like) prior to administering a subsequent dose. The existence of an immune response to the first dose may also be assumed by waiting for a period of time after the first immunization that, based on previous experience, is a sufficient time for an immune response and/or priming to have taken place. Boosting dosages of an immunoprotective composition can be administered as needed.
The immunoprotective compositions are typically administered to an individual that is immunologically naive with respect to F. tularensis. Usually, 2-4 doses of an immunological composition of the invention may be sufficient, however additional doses may be required to achieve a high level of immunity. Additional booster doses may be given every 1-5 years, as necessary, to maintain a high level of immunity.
In general, administration to any individual should begin prior to the first sign of disease, or possibly at the first sign of possible or actual exposure to F. tularensis. 
The toxicity and therapeutic efficacy of the attenuated vaccines provided by the invention are determined using standard pharmaceutical procedures in cell cultures or experimental animals. One can determine the ED50 (the dose therapeutically effective in 50% of the population) using procedures presented herein and those otherwise known to those of skill in the art.
The attenuated vaccines of the invention can be packaged in packs, dispenser devices, and kits for administering genetic vaccines to a mammal. For example, packs or dispenser devices that contain one or more unit dosage forms are provided. Typically, instructions for administration of the compounds will be provided with the packaging, along with a suitable indication on the label that the compound is suitable for treatment of an indicated condition. For example, the label may state that the active compound within the packaging is useful for treating a particular infectious disease, enteric disorder, or for preventing or treating other diseases or conditions that are mediated by, or potentially susceptible to, a mammalian immune response.
The phrase “pharmaceutically acceptable” refers to molecular entities and compositions that are physiologically tolerable and do not typically produce an allergic or similar undesirable reaction, such as gastric upset, dizziness, fever and the like, when administrated to a human. Preferably, as used herein, the term “pharmaceutically acceptable” means fulfilling the guidelines and approval criteria of a European Community country's Drug Registration Agency concerning products to be used as a drug, or means that the pharmaceutically acceptable compound, composition, method or use, is listed in the European Community country's Pharmacopoeia or other generally recognised pharmacopoeia for use in animals, and more particularly in humans.
The term “pharmaceutical carrier” refers to a diluent, adjuvant, excipient, or vehicle with which the compound is administered. Such pharmaceutical carriers include but are not limited to sterile liquids, such as water and oils, including those of petroleum, oil of animal-, vegetable-, or synthetic origin, such as whale oil, sesame oil, soybean oil, mineral oil and the like. Water or aqueous solutions, saline solutions, and aqueous dextrose and glycerol solutions are preferably employed as carriers, particularly for injectable solutions, droplet-dispensed solutions and aerosols.
The term “adjuvant” refers to a compound or mixture that enhances the immune response to an antigen. An adjuvant can serve as a tissue depot that slowly releases the antigen and also as a lymphoid system activator that non-specifically enhances the immune response (Hood et al., Immunology, Second Ed., 1984, Benjamin/Cummings: Menlo Park, Calif., p. 384). Often, a primary challenge with an antigen alone, in the absence of an adjuvant, will fail to elicit a humoral or cellular immune response. Adjuvants include, but are not limited to, complete Freund's adjuvant, incomplete Freund's adjuvant, saponin, mineral gels such as aluminum hydroxide, surface active substances such as lysolecithin, pluronic polyols, polyanions, peptides, oil or hydrocarbon emulsions, keyhole limpet hemocyanins, dinitrophenol, and potentially useful human adjuvants such as BCG (bacille Calmette-Guerin) and Corynebacterium parvum. Preferably, the adjuvant is pharmaceutically acceptable.
The primary purpose of an adjuvant is to enhance the immune response to a particular antigen of interest. In the context of antibody production for research purposes, adjuvants stimulate the rapid and sustained production of high titers of antibodies with high avidity. This permits ready recovery of antibody for further research in vitro. Adjuvants have the capability of influencing titer, response duration, isotype, avidity and some properties of cell-mediated immunity.
Adjuvants may act through three basic mechanisms. The first is to enhance long term release of the antigen by functioning as a depot. Long term exposure to the antigen should increase the length of time the immune system is presented with the antigen for processing as well as the duration of the antibody response. The second is the interaction the adjuvant has with immune cells. Adjuvants may act as non-specific mediators of immune cell function by stimulating or modulating immune cells. Adjuvants may also enhance macrophage phagocytosis after binding the antigen as a particulate (a carrier/vehicle function).
Selection of an adjuvant is based upon antigen characteristics (size, net charge and the presence or absence of polar groups). Adjuvant choice is also dependent upon selection of the species to be immunized. Adjuvant selection remains largely empirical. Antigens that are easily purified or available in large quantities may be good choices for starting with the least inflammatory adjuvants for immunization. Should antibody response not be suitable, a gradual increase in the inflammatory level of the adjuvant would then be warranted. Antigens which are difficult to come by (e.g., very small quantities are available) may be better choices for complexing with the more inflammatory adjuvants such as CFA. In addition, small molecular weight compounds and others known to be weakly immunogenetic, may need to be complexed with CFA to obtain good antibody titers. Exemplary adjuvants include:
Complete Freund's Adjuvant (CFA) is a mineral oil adjuvant that uses a water-in-oil emulsion which is primarily oil. It generally contains paraffin oil, killed mycobacteria and mannide monoosleate. The paraffin oil is generally not metabolized; it is either expressed through the skin (via a granuloma or abscess) or phagocytized by macrophages.
Incomplete Freund's Adjuvant (IFA) is a mineral oil adjuvant with a composition similar to CFA but lacking killed mycobacteria.
Montanide ISA (incomplete seppic adjuvant) is mineral oil adjuvant that uses mannide oleate as the major surfactant component.
Ribi Adjuvant System (RAS) is an oil-in-water emulsion that contains detoxified endotoxin and mycobacterial cell wall components in 2% squalene.
TiterMax is a water-in-oil emulsion that combines a synthetic adjuvant and microparticulate silica with the metabolizable oil squalene. The copolymer is the immunomodulator component of the adjuvant. Antigen is bound to the copolymer and presented to the immune cells in a highly concentrated form.
Syntex Adjuvant Formulation (SAF) is a preformed oil-in-water emulsion that uses a block copolymer for a surfactant. A muramyl dipeptide derivative is the immunostimulatory component. The components are subsequently included in in squalene, a metabolizable oil.
Aluminum Salt Adjuvants are most frequently used as adjuvants for vaccine antigen delivery and are generally weaker adjuvants than emulsion adjuvants.
Nitrocellulose-adsorbed antigen provides the slow degradation of nitrocellulose paper and prolonged release of antigen.
Encapsulated or entrapped antigens permit prolonged release of antigen over time and may also include immunostimulators in preparation for prolonged release.
Immune-stimulating complexes (ISCOMs) are antigen modified saponin/cholesterol micelles. They generally form stable structures that rapidly migrate to draining lymph nodes. Both cell-mediated and humoral immune responses are achieved. Quil A and QS-21 are examples of ISCOMS.
GerbuR is an aqueous phase adjuvant and uses immunostimulators in combination with zinc proline.
The following examples are provided to further illustrate the invention and are not intended to limit the invention. Those of skill in the art will recognize that specific materials used in the methods and compositions may be substituted with equivalent materials.
EXAMPLES
Example 1
Cell lines, bacteria, and mice. Human macrophage-like THP-1 cells (ATCC TIB-202) were cultured in RPMI 1640 containing penicillin (100 μg/ml) and streptomycin (100 U/ml) and supplemented with 10% FBS. F. tularensis LVS and SchuS4 were obtained from the Centers for Disease Control and Prevention (Atlanta, Ga.). The bacteria were passaged once on monolayers of THP-1 cells, followed by amplification on chocolate II agar (BD BBL, Sparks, Md.) for 3 days. The bacteria were then scraped from colonies on plates, suspended in sterile saline in the presence of 20% glycerol, and stored at −80° C. Before each use in animals, one vial of LVS or SchuS4 was thawed immediately at 37° C., diluted in sterile saline and kept on ice until use.
Six to eight week old specific-pathogen-free female BALB/c mice were purchased from Charles River Laboratory (Wilmington, Mass.).
Intranasal Challenge.
Two LPS mutants, LVSΔwbtDEF and LVSΔwzy, were constructed as vaccine vectors. These vectors were highly attenuated in mice. They protected mice against challenge with intranasal LVS; however, the level of protection was orders of magnitude less than that of LVS, as assessed by assaying the burden of Ft LVS in the lung, spleen, and liver after challenge (FIG. 1). Thus, another deletional mutant was designed. A putative capsule-deficient mutant of LVS was used.
Construction of a Live Attenuated Recombinant LVS Putatively Deficient in Capsule Production (LVSΔcapB).
The LVSΔcapB mutant was obtained by allelic exchange. Briefly, 1-kb upstream and downstream genomic DNA fragments flanking the capB gene of LVS were amplified and ligated via the EcoRI site to form an in-frame capB gene deletion cassette. The deletion cassette was cloned into the suicide plasmid pMP590, bearing a kanamycin resistance gene and a sacB gene and delivered into LVS by electroporation. LVS transformants were subjected to sequential selection on chocolate agar containing kanamycin and 5% sucrose. The resulting LVSΔcapB mutant does not carry any antibiotic-resistance gene. The capB deletion was confirmed by chromosomal sequencing.
Surprisingly, in view of its marked attenuation, LVSΔcapB induces protective immunity comparable to LVS against lethal i.n. challenge with F. tularensis. To examine the capacity of LVSΔcapB to induce protective immunity against lethal challenge with F. tularensis, mice were immunized with well-tolerated doses of LVSΔcapB i.n. or i.d., and 4 weeks later challenged with 4000 CFU of LVS i.n., a route chosen to mimic respiratory exposure. Sham-immunized mice and mice immunized with moderately well-tolerated doses of LVS (150 CFU i.n. and 1×105 or 1×106 CFU i.d.) served as controls. Five days after challenge, the peak time of bacterial growth in the host, mice were euthanized; the spleen, liver and lung removed and homogenized in sterile saline; the homogenate serially diluted and plated on CHA-HB plates; the plates incubated at 37° C. for 3 days; and CFU enumerated. As shown in FIG. 5, in mice immunized with LVSΔcapB i.n. or i.d., the bacterial burden in the spleen and liver was nearly 4 logs lower and the bacterial burden in the lung was 3-4 logs lower than in sham-vaccinated animals and comparable to the bacterial burden in mice immunized with LVS. This indicated that both local replication in the lung and systemic dissemination of F. tularensis was strongly inhibited by LVSΔcapB immunization.
This result was in marked contrast to the results obtained with the LVSΔwbtDEF mutant (FIG. 1). In animals immunized with LVS or the LVSΔwbtDEF mutant and then challenged with intranasal LVS, the bacterial burden in the lungs, liver, and spleen was markedly reduced in LVS-immunized animals, but not in LVSΔwbtDEF immunized animals (FIG. 1). Whereas LVS-immunized animals showed a reduction in CFU in the lung, liver, and spleen of 3-5 logs compared with sham-immunized animals, LVSΔwbtDEF—immunized animals showed a reduction of only  ̃1 log in the lung and 2-3 logs in the spleen and liver (FIG. 1). In marked contrast, the reduction in organ bacterial burden in animals immunized with LVSΔcapB was comparable to that in animals immunized with LVS (FIG. 5).
Construction of Recombinant LVSΔcapB Expressing F. tularensis Proteins.
Using LVSΔcapB as a parental strain, the following LVSΔcapB mutants over-expressing the virulent F. tularensis antigens including IglC were generated. LVSΔcapB/Pgro-IglC: To construct plasmids that can replicate in both E. coli and F. tularensis strains, the pFNLTP6/gro-gfp plasmid was used as a delivery vector, which contains a kanamycin resistant gene and is engineered to grow in F. tularensis LVS, F. novicida 112, and E. coli. The coding sequence for F. tularensis SchuS4 IglC was amplified by PCR from the genomic DNA of an F. tularensis subspecies tularensis RCI (recent clinical isolate) and cloned into pZErO. The inserted sequence was confirmed by nucleotide sequencing before it was subcloned into the NdeI-BamHI sites of the pFNLTP6/gro-gfp vector. The resultant plasmid with the insertion of an expression cassette for virulent F. tularensis IglC, pFNLTP6/gro-iglC, was then electroporated into the LVSΔcapB mutant. The bacteria mixture was selected on chocolate agar containing kanamycin (10 μg/ml). The parental capB-deficient LVS mutant is sensitive to kanamycin and therefore cannot grow in the presence of kanamycin. Under the selective pressure of kanamycin, only LVS mutants carrying the transfer vector pFNLTP6/gro-IglC were able to grow on the chocolate agar containing kanamycin (10 μg/ml). After 3 days incubation, the colonies were screened by PCR amplification using two pairs of primers. The first pair was designed to anneal to the 3′end of the groE promoter and the 5′end of IglC coding sequence, which would amplify a PCR product of approximately 670 bp from the LVS mutant carrying the transfer vector. As expected, a 670-bp PCR product was amplified from the selected LVS mutants, but was missing from the parental LVS strain. The second pair of primers was designed to anneal specifically to the 5′ and 3′ ends of the groE promoter, which would generate a 421-bp PCR product from the parental transfer vector pFNLTP6/gro-gfp and 1035 bp PCR product from the transfer vector with the IglC expression cassette. As expected, the selected LVS strains generated a PCR product of 1035 bp. These results indicated that the selected capB deficient LVS strain contained the transfer vector pFNLTP6/Pgro-IglC. The selected strain was named Ft LVSΔcapB/Pgro-IglC. The confirmed LVSΔcapB mutants were then amplified on chocolate agar plates containing kanamycin (10 μg/ml).
The LVSΔcapB/Pgro-IglC strain amplified from chocolate agar was washed once with sterile saline, resuspended in 20% glycerol/saline solution, and then stored at −80° C. until use. Upon each use, the stock was thawed immediately at 37° C. and diluted in sterile saline.
LVSΔcapB/Pgro-AcpASS-IglC:
An F. tularensis protein fused to a signal peptide was designed to be expressed and secreted into the cytoplasm, such that the F. tularensis protein would be presented to MHC class I molecules and induce immune responses specific to the F. tularensis antigen. To construct such a fusion protein, IglC was fused with the signal sequence of F. tularensis AcpA. The coding sequence for IglC was fused with that for the N-terminal 28 amino acids (signal sequence plus 5 extra peptides) of AcpA by PCR, cloned the DNA fragment into the NdeI-BamHI sites of the pFNLTP6/gro-gfp plasmid downstream of the groE promoter, and engineered into the LVSΔcapB mutant as described above for the LVSΔcapB/Pgro-IglC strain. The resultant strain, LVSΔcapB/Pgro-AcpASS-IglC, was confirmed by colony PCR and amplified on chocolate agar plates containing kanamycin (10 μg/ml).
LVSΔcapB/Pgro-IglA:
The LVSΔcapB/Pgro-IglA strain was constructed as described above for LVSΔcapB/Pgro-IglC.
LVSΔcapB/PiglA-IglA:
Although it has no predicted signal sequence in the N-terminus, IglA is a cytoplasmic protein of F. tularensis under in vitro growth conditions. To examine whether the endogenous promoter of IglA would enhance the expression and secretion of IglA, the promoter and coding sequence for IglA was amplified by PCR and cloned into pZErO. The inserted sequence was confirmed by nucleotide sequencing and subcloned into the KpnI-BamHI sites of pFNLTP6/gro-gfp plasmid in place of the DNA sequence for the groE promoter and gfp. The resultant plasmid, pFNLTP6/PiglA-iglA, was introduced into LVSΔcapB to generate LVSΔcapB/PiglA-IglA as described above for LVSΔcapB/Pgro-IglC.
LVSΔcapB/Pgro-VgrG: The LVSΔcapB/Pgro-VgrG strain was constructed as described above for LVSΔcapB/Pgro-IglC.
LVSΔcapB/Pgro-IglB: The LVSΔcapB/Pgro-IglB strain is being constructed as described above for LVSΔcapB/Pgro-IglC.
Results:
(i) LVSΔcapB, which retains the O-Antigen, was serum resistant. (ii) In a competition experiment, LVSΔcapB was outgrown by parental LVS in THP-1 cells. (iii) LVSΔcapB was significantly attenuated in mice and caused no weight loss, obvious signs of illness, or deaths at any dose tested; hence the LD50 i.n. was >107 CFU vs. 1.8×103 CFU for LVS and the LD50 i.d. was >108 CFU vs. 3.2×107 CFU for LVS. (iv) Mice immunized with LVSΔcapB i.n. or i.d. and then challenged 4 weeks later with a lethal dose of LVS i.n. were 100% protected from illness and death. In mice immunized with LVSΔcapB, the bacterial burden in the lung was 3-5 logs lower than in sham-immunized animals, and the bacterial burden in the spleen and liver was 3-4 logs lower than in sham-immunized animals, comparable to that in mice immunized with LVS. This indicated that both local replication in the lung and systemic dissemination of F. tularensis was strongly inhibited by immunization with LVSΔcapB.
Example 2
Competition Assay for Growth in Human Macrophages.
To compare the growth of LVSΔcapB with its parental LVS strain in human macrophages, a pair of LVS strains carrying a kanamycin-resistance gene (LVS-kan) or a hygromycin-resistance gene (LVS-hyg) were constructed and a comparable pair of LVSΔcapB strains each carrying one of the same antibiotic resistance genes (LVSΔcapB-kan and LVSΔcapB-hyg). THP-1 cells were co-infected with LVSΔcapB-hyg and LVS-kan or co-infected with the same two strains carrying the opposite resistance markers (LVSΔcapB-kan and LVS-hyg). At various times post infection, the cell monolayer was lysed, serially diluted, and plated on chocolate agar supplemented with either kanamycin or hygromycin. Colony Forming Units (CFU) were enumerated and the ratio between the number of LVSΔcapB and LVS was calculated at each time point.
Analysis of Bacterial Lipopolysaccharide (LPS) and Protein Expression by Coomassie Blue Staining and Western Blotting.
LPS expression by the LVSΔcapB mutant was analyzed by Western blotting using the monoclonal antibody FB11 (Abcam Inc. MA), at a dilution of 1:5,000 and subsequently horseradish peroxidase-conjugated goat anti-mouse immunoglobulin G (Bio-Rad) at a dilution of 1:25,000. The blots were incubated with chemiluminescent substrate (Pierce) and the proteins were detected by exposing film to the blot. Protein expression by LVSΔcapB mutant was analyzed by Coomassie blue staining.
Vaccination and Challenge of Mice.
Mice were vaccinated by the i.d. or intranasal (i.n.) route. For i.d. vaccination, mice were shaved, decontaminated with 70% ethanol at the base of the tail and injected using 27G 1⁄2 cc Tuberculin syringes (Becton Dickson, N.J.) with 50 μL sterile phosphate buffered saline (PBS, Negative control), or with various doses of LVS (Positive Control) or LVSΔcapB diluted in 50 μl PBS. For i.n. vaccination, mice were anesthetized by intraperitoneal (i.p.) injection with Ketamine (80 mg/kg) and Xylazine (10 mg/kg), and vaccines were administered into the nostrils in a total volume of 20 μl sterile saline. At various times after vaccination, mice were either euthanized for immunology studies or challenged by the i.n. route at UCLA with 4000 CFU LVS (>5×LD50), administered in the same way as i.n. vaccination, or by the aerosol route with 10×LD50 of the Type A F. tularnesis SchuS4 strain. The aerosol challenge was conducted in a chamber of 5 cubic feet with the mice conscious and active, using a Glas-Col Inhalation Exposure System (Glas-Col, LLC, Terre Haute, Ind.). Challenged mice were weighed and monitored for illness and death for 3 weeks. Mice that met pre-determined humane endpoints for euthanasia were euthanized and counted as a death. Mean Survival Time (MST) was calculated by dividing the sum of the surviving days of all mice by the total number of mice examined, with animals surviving until the end of the experiment given a survival time of 21 days, when the experiment was terminated.
To quantitate bacterial burden in host tissues, mice were euthanized at 5 days post-infection. Liver, spleen, lung, and in the case of i.d. immunization, a 1-cm2 area of local skin at the injection site were removed aseptically and immediately weighed, immersed in 2 ml sterile PBS and kept on ice until homogenization. The homogenization was performed using a Pro200 Homogenizer (PRO Scientific Inc. Oxford, Conn.). Serial 10-fold dilutions of the homogenates were plated onto Chocolate agar plates containing sulfamethoxazole (40 μg/ml), trimethoprim (8 μg/ml) and erythromycin (50 μg/ml), and incubated in a 5% CO2 atmosphere at 37° C. for three days before colonies were enumerated.
Assay for Lymphocyte Proliferation.
Groups of four BALB/c mice were sham-immunized or immunized i.d. with 1×105 CFU LVS or 1×106 CFU LVSΔcapB or immunized i.n. with 200 CFU LVS or 1×105 CFU LVSΔcapB. At four weeks post immunization, mice were anesthetized by i.p. injection of Ketamine and Xylazine, bled and then euthanized. Sera were collected for serum antibody measurement as described below. Spleens were removed and a single cell suspension of splenocytes was prepared. Lymphocyte proliferation in response to heat-inactivated LVS antigen was assayed. Splenocytes from each animal were cultured in triplicate and stimulated with heat-inactivated LVS for 48 hours. Two hours before harvesting, the stimulated splenocytes were pulsed with methyl-[3H]thymidine and harvested with a cell harvester (Skatron). The amount of incorporated [3H]thymidine was determined by counting in a liquid scintillation counter.
Heat-inactivated LVS antigen was prepared for this assay by growing LVS on Chocolate II agar for 2 days, scraping the bacteria into PBS, and incubating at 80° C. for 1 hour. Loss of viability was confirmed by plating the antigen onto Chocolate II agar plates and incubating at 37° C. for 2 days. No colonies were detected on the plates, indicating that no viable bacteria survived heat-inactivation.
Serum Antibody Detection by Enzyme-Linked Immunosorbent Assay (ELISA).
Sera collected from sham-immunized mice or mice immunized with LVS or LVSΔcapB were analyzed for levels of IgM, IgA, IgG, IgG1 and IgG2a antibodies specific for LVS. The heat-inactivated LVS antigen was diluted in carbonate/bicarbonate buffer (50 mM NaHCO3, 50 mM Na2CO3) to an optical density of 0.025 at 540 nm (equivalent to 5×106 CFU/0.1 ml) and 0.1 ml was used to coat 96-well high-binding capacity plates (Corning, N.Y.). Excess antigen was removed by washing three times with PBS. Sera at a starting dilution of 1:32 were diluted further through a two-fold series with PBS containing 1% bovine serum albumin. The diluted sera were incubated with the heat-inactivated LVS coated on 96-well plates at ambient temperature for 3 hours. The plates were subsequently incubated for 90 min at ambient temperature with alkaline phosphatase-conjugated goat anti-mouse IgA (Sigma, St. Louis, Mo.), IgM, IgG1 or IgG2a (Invitrogen, Camarillo, Calif.) at a dilution of 1:1000. The plates then were washed three times with PBS and 0.05% Tween-20. One hundred μl of p-nitrophenylphosphate substrate in diethanolamine buffer (Phosphatase Substrate kit, BioRad, Hercules, Calif.) was added to each well. The yellow color that developed was read at 414 nm for absorbance using a multiscan microplate reader (TiterTek, Huntsville, Ala.). The endpoint antibody titer was calculated as the reciprocal of the highest serum dilution that was a minimum of 0.05 optical density units above the sham-immunized control serum.
Statistical Analysis.
Two-way ANOVA with Bonferroni's post test was performed using GraphPad Prism version 5.01 (San Diego, Calif.) to determine significance in comparisons of mean SIs for lymphocyte proliferative responses, mean antibody titers, and mean Log CFU organ counts among mice in vaccinated and control groups. A log-rank analysis (Mantel-Cox test) using GraphPad Prism version 5.01 was used to determine significance of survival curves among mice in immunized and in sham-immunized control groups.
Construction and In Vitro Characterization of LVSΔcapB, an LVS Mutant Deficient in a Putative Capsule Synthesis Gene.
The resultant LVSΔcapB mutant is free of the antibiotic resistance marker and the truncated capB contains 30 nucleotides encoding 6 amino acids from the N-terminus and 4 amino acids from the C-terminus of CapB (FIG. 6).
The LVSΔcapB Mutant Retains the O-Antigen.
To determine whether LVSΔcapB produces LPS, bacterial lysates of LVS and LVSΔcapB were analyzed by Coomassie blue staining and Western blotting. Similar profiles of protein expression (FIG. 2B) and similar ladder-like patterns of LPS immunoreactivity (FIG. 2A) were observed for LVS and LVSΔcapB, confirming that the deletion of the capB gene did not affect retention of the LPS molecule.
the LVSΔcapB Mutant is not Sensitive to Serum-Killing.
To determine if the LVSΔcapB mutant is sensitive to serum, the strain was incubated with fresh serum and survival assessed by plating on agar (FIG. 3). Unlike the LVSΔwbtDEF mutant, but similar to the parental LVS, the LVSΔcapB mutant was resistant to serum killing.
the LVSΔcapB Mutant is Attenuated for Growth in Macrophages.
To evaluate the attenuation of LVSΔcapB in vitro, the intramacrophage growth of LVSΔcapB was compared with that of LVS in human macrophages in a growth competition assay. The LVSΔcapB mutant carrying either a hygromycin- or kanamycin-resistance marker grew slower than the parental LVS strain carrying the opposite antibiotic resistance marker (kanamycin or hygromycin, respectively) (FIG. 4). This result indicated that the LVSΔcapB mutant is more attenuated than the parental LVS strain in human macrophages.
LVSΔcapB is More Attenuated than Parental LVS in Mice.
As described above, and consistent with the data above, to compare the virulence of LVSΔcapB with the parental LVS in vivo, BALB/c mice were immunized with a) LVSΔcapB at doses ranging from 1×103 to 1×107 CFU i.n. and 1×108 to 1×108 CFU i.d. or b) LVS at doses ranging from 1.5×102 to 4.1×104 CFU i.n. and 1×105 to 1×107 CFU i.d. Control mice were uninfected. The mice were observed for signs of illness, weight loss, death, and, in mice immunized i.d., the extent of a local lesion for three weeks. As shown in FIG. 7A, in mice administered LVS i.n., deaths occurred and at relatively low doses such that the LD50 i.n. was 700 CFU (vs. >107 for LVSΔcapB). All mice immunized i.n. with LVS at each dose tested showed signs of illness (ruffled fur and hunched back) and they had significant weight loss between days 4 and 9 after immunization (FIG. 7A). In contrast, in mice immunized i.n. with LVSΔcapB, 100% of mice survived after immunization at all tested doses, and only transient weight loss occurred at around day 4 post-immunization, at a dose of 1×106 CFU (FIG. 7A) and in a separate experiment at a dose of 1×107 CFU/mouse.
In mice administered LVS i.d., 100% of mice survived after immunization at the doses tested in this experiment (FIG. 7B). However, in a separate experiment, 100% of mice died after i.d. immunization with 1×108 CFU LVS. In addition, mice immunized i.d. with 1×107 LVS developed severe local lesions. In contrast, in mice administered LVSΔcapB i.d., 100% of mice survived 106-108 CFU doses (vs. 0% survival at a dose of 108 CFU for LVS). Although mice immunized with LVSΔcapB i.d. at doses of 107 (FIG. 7B) or 108 CFU had transient weight loss, these mice had no other signs of illness and showed only mild local inflammation at the injection site.
In subsequent experiments evaluating the immunogenicity and efficacy of the i.d. route of immunization, doses of 106 CFU of LVSΔcapB and 105 CFU of LVS were generally used. At these doses, LVSΔcapB was non-toxic in mice and caused no apparent inflammation at the site of injection. In contrast, LVS retained significant toxicity, e.g. causing ruffled fur. In subsequent experiments evaluating the i.n. route of immunization, doses of 105 CFU LVSΔcapB and 200 CFU LVS were generally used. Again, at these doses, LVSΔcapB was completely non-toxic, whereas LVS retained significant toxicity resulting in ruffled fur and hunched backs in immunized animals; moreover, in two experiments even this low dose of LVS proved lethal to 25% of immunized animals.
These results demonstrated that LVSΔcapB is at least 10,000-fold more attenuated than LVS after i.n. immunization and substantially more attenuated after i.d. immunization.
LVSΔcapB is Cleared Faster than LVS after Intranasal Infection.
To assess dissemination and clearance of LVSΔcapB, groups of 4 BALB/c mice were immunized i.n. with 112 CFU LVS or 1×105 CFU LVSΔcapB or i.d. with 1×105 CFU LVS or 1×106 CFU LVSΔcapB. At various times post-immunization, mice were euthanized and spleen, liver, lung and skin at the injection site (in the case of i.d. immunization) were assayed for bacterial burden. As shown in FIG. 8A, following i.n. immunization, LVS replication peaked at day 4 in the lung and at day 7 in the spleen and liver; the LVS was cleared by day 22 post-immunization in all three organs examined. In contrast, although the LVSΔcapB mutant was administered at a much higher dose i.n. (105 CFU vs. 112 CFU for LVS), LVSΔcapB replicated at levels significantly lower than LVS at the peak of infection, and it was cleared faster than LVS in all three organs. Following i.d. immunization (FIG. 8B), the LVSΔcapB strain replicated less efficiently in the local injection area than the parental LVS strain. However, LVSΔcapB spread systemically to the liver and spleen, where it was cleared at a similar or greater rate than parental LVS strain. The replication of both LVSΔcapB and its parental LVS in the lung was limited (FIG. 8B).
These results demonstrated that LVSΔcapB is cleared much faster than LVS in mice after i.n. inoculation, and at least as fast as LVS after i.d. inoculation.
Immunization with LVSΔcapB Induces Strong Cell-Mediated and Humoral Immune Responses.
To examine whether immunization with LVSΔcapB induces cell-mediated and humoral immune responses, groups of 4 mice were immunized with LVSΔcapB at doses of 1×105 CFU by the i.n. route or 1×106 CFU by the i.d. route, doses that were non-toxic (FIG. 7) and that provided excellent protection against LVS i.n. challenge (see below). At 4 weeks post immunization, mice were anesthetized, bled and euthanized (FIG. 9A) and cell mediated and humoral immune responses assayed. With respect to cell-mediated immunity, immunization with LVSΔcapB i.n. or i.d. induced LVS-specific splenic lymphocyte proliferative responses that were significantly higher than those in sham-immunized mice and comparable to those in LVS-immunized mice (FIG. 10). With respect to humoral immune responses, following i.n. immunization, LVSΔcapB induced significantly higher levels of IgG antibody than LVS, predominantly IgG2a, within 7 days post immunization. IgG1 and IgG2a antibodies were detected in all mice immunized i.n. or i.d. with LVS or LVSΔcapB 14 days after immunization (FIG. 11A, B, C). Following i.n. or i.d. immunization, there were no significant differences in IgA and IgM antibody levels between mice immunized with LVS and those immunized with LVSΔcapB except that mice immunized i.n. with LVS had a transiently elevated IgA antibody level at 14 days post-immunization (FIGS. 11D and E).
Thus, LVSΔcapB, while highly attenuated, induces cellular and humoral immune responses comparable to those induced by LVS.
Immunization with LVSΔcapB Protects Mice from Lethal Intranasal Challenge with LVS.
To examine the capacity of LVSΔcapB to induce protective immunity against lethal challenge with F. tularensis, mice were immunized i.n. or i.d with LVSΔcapB at various doses (FIGS. 9B, 9C, and 5). Sham-immunized mice and mice immunized with moderately well-tolerated doses of LVS (150 CFU i.n. and 1×105 or 1×106 CFU i.d.) served as controls. At 4 weeks post-immunization, mice were challenged i.n. with 4000 CFU LVS (>5×LD50). Five days after challenge, approximately the peak time of bacterial growth in the host with an LVS i.n. challenge dose of 4000 CFU, mice were euthanized and the liver, spleen and lung were harvested and assayed for bacterial burden (FIG. 9B). As shown in FIG. 5, in mice immunized with LVSΔcapB i.n. or i.d., the bacterial burden in the spleen and liver was nearly 4 logs lower and the bacterial burden in the lung was 3-4 logs lower than in sham-immunized animals and comparable to the bacterial burden in mice immunized with LVS. The protection induced by immunization with LVSΔcapB was dose-dependent. Among all the doses of LVSΔcapB tested, 1×105 CFU i.n. and 1×106 CFU i.d. were both well-tolerated and induced protection in mice against F. tularensis challenge comparable to LVS. Therefore, in subsequent challenge experiments and the experiments described above in which immune responses were evaluated, these doses were used to immunize mice.
The above results indicated that both local replication in the lung and systemic dissemination of F. tularensis is strongly inhibited by immunization with LVSΔcapB.
To further determine if immunization with LVSΔcapB can provide protection in mice against lethal LVS challenge, groups of 12 mice were immunized once i.n. with 200 CFU LVS (the maximum dose mice can usually survive) or 1×105 CFU LVSΔcapB, or once i.d. with 1×105 CFU LVS or 1×106 CFU LVSΔcapB. Notably, 25% ( 3/12) of mice immunized i.n. with just 200 CFU LVS died within 10 days of immunization, further underscoring the toxicity of LVS. In contrast, mice immunized with LVSΔcapB i.n. or i.d. did not shown any signs of illness. At 4 weeks post immunization, surviving mice were challenged i.n. with 4000 CFU LVS (>5×LD50). Four of 12 mice in each group were euthanized at 5 days post challenge and lung, liver and spleen were removed for assay of bacterial burden. The remaining 8 mice in each group (except the LVS i.n. group, where only 5 mice remained) were monitored for signs of illness, weight loss and death for 3 weeks (FIG. 9C). Mice immunized i.n. or i.d. with LVSΔcapB and challenged 4 weeks later had lung bacterial burdens >4 logs lower after i.n. immunization and 2 logs lower after i.d. immunization than sham-immunized mice; CFU in the spleen was below the limit of detection after i.n. immunization and was 4 logs lower than sham-immunized mice, similar to mice immunized with LVS; and CFU in the liver were below the limit of detection after both i.d. and i.n. immunization, similar to mice immunized with LVS (FIG. 12A). Importantly, the bacterial burden in mice immunized with LVSΔcapB was 3 logs lower than in mice immunized with the LPS-deletion mutant LVSΔwbtDEF. Although mice immunized with LVSΔcapB had a somewhat higher bacterial burden in the lung than mice immunized with LVS, these mice showed no signs of illness after challenge. In the survival part of the study, 100% of mice immunized i.n. or i.d. with LVSΔcapB and challenged with LVS i.n. at 4 weeks post-immunization survived, the same as for mice immunized i.n. or i.d. with LVS (FIG. 12C).
To evaluate the protection provided by immunization with LVSΔcapB after a longer immunization-challenge interval, groups of 12 mice were immunized i.n. or i.d. with LVS or LVSΔcapB as described above and challenged i.n. with 4000 CFU LVS at 8 weeks post immunization. Again, 25% ( 3/12) of mice immunized i.n. with LVS died within 10 days of immunization, consistent with the results described above. At 5 days post challenge, mice immunized with LVSΔcapB i.n. or i.d. had significantly lower bacterial burdens than sham-immunized mice in all three organs examined (FIG. 12B). In animals immunized i.n., LVS-immunized animals had significantly fewer CFU in the spleen and lung than LVSΔcapB-immunized animals. In the liver, there was no significant difference in CFU between animals immunized with LVS and LVSΔcapB. In animals immunized i.d., there was no significant difference in CFU between LVSΔcapB- and LVS-immunized animals in any of the three organs assayed.
In the survival part of the study (FIG. 12D), all 8 sham-immunized animals died whereas all animals immunized with LVSΔcapB or LVS i.n. or i.d. survived (8 per group except the group immunized i.n. with LVS, where only 5 animals survived immunization).
Thus, LVSΔcapB, while much less virulent than LVS, induces potent protective immunity against lethal LVS i.n. challenge, comparable to that induced by LVS.
Immunization with LVSΔcapB protects mice from aerosol challenge with the virulent F. tularensis SchuS4 strain. To further define the protective immunity induced by immunization with LVSΔcapB, the capacity of the vaccine to protect against the highly virulent F. tularensis SchuS4 strain was analyzed. Groups of 8 mice were immunized once i.n. with 1×105 CFU LVSΔcapB or i.d. with 1×106 CFU LVSΔcapB, challenged the mice 6 weeks later by aerosol with 10×LD50 F. tularensis SchuS4, and monitored the animals for weight change, signs of illness, and death for 3 weeks (FIG. 9D). Mice sham-immunized with PBS or immunized with LVS (200 CFU i.n. or 1×105 CFU i.d.) served as controls. Mice immunized with LVSΔcapB developed strong protective immunity to F. tularensis SchuS4 challenge (FIG. 13). In mice immunized i.d. (FIG. 13A), protection was incomplete. All LVSΔcapB and sham-immunized mice succumbed to challenge; however, LVSΔcapB-immunized mice survived twice as long (10 days) as sham-immunized mice (5 days, P<0.0001). LVS-immunized mice were better protected than LVSΔcapB-immunized mice (62.5% vs. 0% survival); however, all these mice showed substantial weight loss after challenge.
In mice immunized with LVSΔcapB i.n. (FIG. 13B), 100% of the mice survived challenge vs. 0% for sham-immunized animals (p<0.0001). Similarly, 100% of LVS-immunized animals survived. Notably, the LVSΔcapB and LVS-immunized animals showed no signs of illness after challenge, e.g. no weight loss.
Thus LVSΔcapB induces potent protective immunity to F. tularensis SchuS4 aerosol challenge, after both i.d. and i.n. immunization. Protection after i.n. immunization is stronger than after i.d. immunization and comparable to that induced by LVS.
Example 3
To examine the capacity of the attenuated rLVSΔcapB vaccines expressing F. tularensis proteins (IglA, IglC, VgrG, or Tul4) to induce protective immunity against lethal challenge with F. tularensis, mice were immunized with well-tolerated doses of LVSΔcapB, LVSΔcapB/Pgro-IglA, LVSΔcapB/PiglA-IglA, LVSΔcapB/Pgro-IgC, LVSΔcapB/Pgro-AcpASS-IgC, LVSΔcapB/Pgro-VgrG, or LVSΔcapB/Pgro-Tul4 intranasally (i.n.) or intradermally (i.d.). Sham-immunized mice and mice immunized with LVS (200 CFU i.n. and 1×105 CFU i.d.) served as controls. Four weeks later, the mice were challenged with 4000 CFU of LVS i.n. (>5×LD50). Five days after challenge, the peak time of bacterial growth in the host, mice were euthanized; the spleen, liver and lung removed and homogenized in sterile saline; the homogenate serially diluted and plated on CHA-HB plates; the plates incubated at 37° C. for 3 days; and CFU enumerated. As shown in FIG. 14, in mice immunized with LVSΔcapB i.n., the bacterial burdens in the spleen and liver were >4 logs lower and the bacterial burden in the lung was >3 logs lower than in sham-vaccinated animals. These results were consistent with the previous data, further demonstrating that both local replication in the lung and systemic dissemination of F. tularensis was strongly inhibited by immunization with the attenuated LVSΔcapB strain. Importantly, mice immunized i.n. with LVSΔcapB/Pgro-IgC, LVSΔcapB/Pgro-IglA, or LVSΔcapB/PiglA-IglA had lower bacterial burdens in the lung than mice immunized with the parental LVSΔcapB strain, indicating that rLVSΔcapB vaccines expressing F. tularensis proteins induce greater protective immunity than the parental LVSΔcapB against lethal challenge with F. tularensis (FIG. 15A). Similarly, mice immunized i.d. with rLVSΔcapB vaccines, including LVSΔcapB/Pgro-IgC, LVSΔcapB/PiglA-IglA, LVSΔcapB/Pgro-VgrG, LVSΔcapB/Pgro-Tul4, had lower bacterial burdens in the lung than mice immunized with the LVSΔcapB parental strain (FIG. 14B).
Example 4
To confirm further the protective immunity induced by the rLVSΔcapB expressing IglA or IglC, the LVSΔcapB/Pgro-IgC and LVSΔcapB/Pgro-IglA vectors that induced greater protective immunity than the parental LVS LVSΔcapB strain were further analyzed. Groups of 4 mice were immunized i.n. or i.d. as described above in Example 3. 2 out 4 mice immunized i.n. with LVS died 7 days after immunization, confirming the toxicity of the LVS strain, even at the low dose used. However, mice immunized with LVSΔcapB, LVSΔcapB/Pgro-IgC or LVSΔcapB/Pgro-IglA did not show any signs of illness, confirming the safety of these vaccines. Four weeks after immunization, mice were challenged with 4000 CFU of LVS i.n. (>5×LD50) and assayed for organ bacterial burden at 5 days post challenge. As shown in FIG. 15, in mice immunized i.n. with LVSΔcapB/Pgro-IgC or LVSΔcapB/Pgro-IglA, the bacterial burden in the lung and spleen was lower than in mice immunized with the parental LVSΔcapB strain. More importantly, in mice immunized with LVSΔcapB/Pgro-IgC or LVSΔcapB/Pgro-IglA via the i.d. route, a route that is safer than the i.n. route but also one by which it is more difficult to induce protective immunity, the bacterial burdens in the lung, spleen and liver were also lower than in mice immunized with the parental LVSΔcapB strain.
Example 5
To evaluate whether rLVSΔcapB vaccines expressing F. tularensis proteins induce longer-term immunity against high dose F. tularensis challenge, groups of 4 mice were immunized i.n. or i.d. with LVS, LVSΔcapB, LVSΔcapB/Pgro-IgC or LVSΔcapB/Pgro-IglA as described above in Example 3 and 4. Again, 2 out of 4 mice died 7 days after i.n. immunization with LVS. Eight weeks later, mice were challenged with 6.3×104 CFU LVS i.n. (≈90×LD50) and monitored for weight loss and other signs of illness daily. At 5 days post challenge, mice were euthanized and the bacterial burdens in lung, spleen, and liver were determined. As shown in FIGS. 17A and 17B, the sham-immunized mice had significant weight loss after challenge. In contrast, mice immunized i.n. with LVSΔcapB, LVSΔcapB/Pgro-IgC or LVSΔcapB/Pgro-IglA did not show signs of illness after challenge—nor did the two mice that survived i.n. immunization with LVS (FIG. 16A). Notably, mice immunized i.d. with the parental LVSΔcapB showed substantial weight loss after challenge, whereas mice immunized i.d. with LVSΔcapB/Pgro-IgC or LVSΔcapB/Pgro-IglA did not show weight loss or other signs of illness, comparable to mice immunized with LVS (FIG. 16B). Importantly, in mice immunized i.n. or i.d. with LVSΔcapB/Pgro-IgC or LVSΔcapB/Pgro-IglA, the bacterial burdens in the lung, spleen, and liver were consistently lower than in mice immunized with the parental LVSΔcapB strain, and comparable to the levels in mice immunized with LVS (FIG. 17). Thus, overexpressing F. tularensis proteins, especially IglA and IglC, in the parental LVSΔcapB vector yields vaccines that are of greater potency than the parental LVSΔcapB vector. These results further confirmed demonstrate that an attenuated homologous vector overexpressing immunogenic proteins of a specific intracellular pathogen induces greater protective immunity than the parental vector alone.
Example 6
To further evaluate rLVSΔcapB vaccines expressing F. tularensis proteins, groups of 24 BALB/c mice were sham-immunized or immunized by the i.d. route with LVS (targeted dose 1×105 CFU/mouse), or with LVSΔcapB, LVSΔcapB/Pgro-IgA or LVSΔcapB/Pgro-IglC (1×105 CFU/mouse). Groups of 8 BALB/c mice were also immunized by the i.n. route with LVS (targeted dose 200 CFU/mouse), or LVSΔcapB, LVSΔcapB/Pgro-IgC or LVSΔcapB/Pgro-IglA (targeted dose 1×105 CFU/mouse). The immunization dose actually delivered for each vaccine was determined by plating serial dilutions of the inocula onto Chocolate agar. After immunization, mice were monitored for weight change and signs of illness. As shown in FIG. 18A, mice immunized with 2×105 CFU LVS had significant weight loss at day 3 post-immunization. In contrast, mice immunized with 2-4×106 CFU of LVSΔcapB, LVSΔcapB/Pgro-IgA or LVSΔcapB/Pgro-IglC did not show signs of illness, including weight loss, after immunization. As shown in FIG. 18B, mice immunized with 236 CFU LVS i.n. had significant weight loss between days 4 and 10 post-immunization and 2 out of 8 mice in this group died after immunization. Mice immunized with LVSΔcapB/Pgro-IglC had transient weight loss around day 4 post-immunization. Mice immunized with LVSΔcapB or LVSΔcapB/Pgro-IglA did not show significant weight loss after immunization. Although the rLVSΔcapB vaccines were administered i.d. at doses 20-40 times higher than LVS and administered i.n. at doses  ̃800-1600-fold higher than LVS, the rLVSΔcapB vaccines were significantly less toxic than LVS.
Example 7
Groups of 8 mice were sham-immunized, immunized i.d. (A) with 1×105 CFU LVS or 1×106 CFU LVSΔcapB, rLVSΔcapB/IglA, or rLVSΔcapB/IglC or immunized i.n. (B) with 200 CFU LVS or 1×105 CFU LVSΔcapB, rLVSΔcapB/IglA or rLVSΔcapB/IglC. Six weeks later, all mice were challenged by the aerosol route with 10×LD50 of the F. tularensis SchuS4 strain. Mice were monitored for survival for 3 weeks. Mean survival time was calculated by dividing the sum of the survival times of all mice in a group by the total number of mice challenged, with animals surviving until the end of the experiment given a survival time of 21 days, when the experiment was terminated. The difference in survival between the mice in the vaccinated groups and mice in the sham-vaccinated group were evaluated using a log-rank (Mantel-Cox) test (Prism 5). As shown in FIG. 13A, mice immunized i.d. with rLVSΔcapB/IglA or rLVSΔcapB/IglC had a greater survival rate and survived longer (MST) than mice immunized with the parental LVSΔcapB (P=0.01 and 0.09, respectively); the survival rate for mice immunized with both vaccines was not significantly different from that of mice immunized with LVS. In mice immunized i.n. with rLVSΔcapB/IglC or rLVSΔcapB/IglA, 100% of mice survived, the same as for mice immunized i.n. with LVS and LVSΔcapB (FIG. 13B). These results show that LVSΔcapB overexpressing a single Ft protein (i.e. IglC or IglA—especially IglA) induces significantly enhanced protective immunity compared with parental LVSΔcapB and protective immunity is comparable to LVS by both the i.n. and more demanding i.d. route.
Example 8
To evaluate a prime-boost vaccination strategy, mice were immunized i.d. with LVSΔcapB or rLVSΔcapB/IglC, boosted them i.d. with rLm/iglC 3 weeks later, and challenged them 6 weeks later with aerosolized Ft SchuS4. Mice sham-immunized or primed i.d. with LVS or LVSΔcapB and not boosted served as controls (FIG. 19). Mice primed i.d. with rLVSΔcapB or rLVSΔcapB/IglC and boosted with rLm/iglC had greater survival and survived longer (MST) than mice immunized i.d. with only parental LVSΔcapB (FIG. 19A, B). None of the surviving mice showed weight loss (FIG. 19C), indicating high level protection; importantly, the survival rate and MST for these mice were greater than for mice immunized i.d. with LVS, although the difference did not reach statistical significance (FIG. 19B). Moreover, in contrast to mice immunized with the prime-boost vaccine, mice immunized with LVS suffered significant weight loss (P<0.05 on Day 7 after challenge; FIG. 19C). These results indicate that heterologous boosting with rLm/iglC significantly enhances the protective immunity induced by i.d. immunization with the parental LVSΔcapB strain, and that the heterologous prime-boost vaccination strategy yields a vaccine that is both safer and more potent than LVS.
The disclosure demonstrates that (i) LVSΔcapB is serum resistant but significantly attenuated in both human macrophages and mice, providing a safer vaccine candidate than LVS. (ii) Immunization with LVSΔcapB by the i.d. or i.n. route induces strong protective immunity, comparable to that induced by LVS at maximum tolerated doses, against lethal i.n. challenge with F. tularensis LVS. (iii) Immunization with LVSΔcapB by the i.d. or i.n. route induces strong protective immunity against aerosol challenge with F. tularensis subspecies tularensis. By the i.n. route, LVSΔcapB induces protection comparable to LVS (100% protection). (iv) Immunization with rLVSΔcapB expressing F. tularensis antigens IglA or IglC by the i.d. or i.n. route induces strong protective immunity against aerosol challenge with F. tularensis subspecies tularensis, comparable to that induced by LVS at maximum tolerated doses. (v) Immunization intradermally with a heterologous prime-boost vaccine utilizing as a prime either LVSΔcapB or rLVSΔcapB/IglC and as a boost rLm/IglC induces strong protective immunity against aerosol challenge with F. tularensis subspecies tularensis, and protection is greater than that induced by LVS at maximum tolerated doses.










Sequence Listing
















acpA
(SEQ ID NO: 1 and 2)


 


bfr
(SEQ ID NO: 3 and 4)


 


dnaK 
(SEQ ID NO: 5 and 6)


(70-kDa heat shock protein)



 


fabD
(SEQ ID NO: 7 and 8)


 


groEL
(SEQ ID NO: 9 and 10)


 


iglC
(SEQ ID NO: 11 and 12)


 


katG
(SEQ ID NO: 13 and 14)


 


pld
(SEQ ID NO: 15 and 16)


 


 


sodB
(SEQ ID NO: 17 and 18)


 


capB
(SEQ ID NO: 19 and 20)


 


 


iglA
(SEQ ID NO: 21 and 22)


 


iglB
(SEQ ID NO: 23 and 24)

## Claims

1. A live attenuated vaccine comprising an F. tularensis lacking a polynucleotide encoding CapB, (LVSΔcapB), and wherein the F. tularensis over-expresses an F. tularensis antigen selected from the group consisting of IglA, IglC and a combination thereof.

2. The live attenuated vaccine of claim 1, wherein the LVSΔcapB overexpresses an immunogenic IglC.

3. The live attenuated vaccine of claim 1, wherein the LVSΔcapB overexpresses an immunogenic IglA.

4. The live attenuated vaccine of claim 1, wherein the LVSΔcapB overexpresses an immunogenic IglA and IglC.

5. An immunogenic composition comprising a live attenuated vaccine of claim 1 useful for inducing an immune response against Francisella tularensis, said antigen comprising an extracellular or immunogenic polypeptide of F. tularensis IglA, IglC or a combination thereof linked to transcriptional promoter and termination signals.

6. The immunogenic composition of claim 5, further comprising a pharmaceutical diluent.

7. The immunogenic of claim 5, wherein the antigen comprises a sequence selected from the group consisting of SEQ ID NO: 12 and 22 and any combination of the foregoing.

8. A recombinant attenuated F. tularensis lacking a polynucleotide encoding CapB, LVSΔcapB and comprising a polynucleotide encoding at least one extracellular or immunogenic protein of F. tularensis selected from the group consisting of IglA, IglC and a combination thereof, wherein the polynucleotide encoding IglA, IglC and a combination thereof is overexpressed and that induces a immunity against F. tularensis.

9. A method of inducing immunity in a subject comprising administering the composition of claim 5 to the subject.

10. A method of immunizing a susceptible host against an infection of Francisella tularensis comprising administering to said host an amount of the immunogenic composition of claim 5 sufficient to invoke an immune response in the host.

11. The method of claim 10, wherein the composition is in a pharmaceutically acceptable carrier.

12. A method of inducing immunity in a subject comprising administering the live attenuated vaccine of claim 1.