U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Crops Pathology and Genetics Research: Davis, CA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Outreach Events
                        UC Davis Picnic Day
                                    Kinders go to College
                                    STEM Girls
                                    Future Scientist Program
                                    Agriculture Future of America
                                    Wallace-Carver Fellowship
                                    Young Scholars Program
                                    Annual Awards Banquet
                    About Us
                        About Us
                                    Our Mission
                                    Principal InvestigatorsSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Pacific West Area Davis, California Crops Pathology and Genetics Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications
- Etiology and Biology of Tree Crop Diseases
- Rice Genetics
- Sustainable Floriculture
- Sustainable Viticulture

Title:   Author  item
                                            SUDARSHANA, PADMA - USDA, ARS, CPGRUitem
                                            McClean, Aliitem
                                            Kluepfel, Daniel
  Submitted to: American Phytopathological Society Abstracts
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 6/15/2006
                                    Publication  Date: 8/1/2006
                                    Citation: Sudarshana, P., Mcclean, A.E., Kluepfel, D.A. 2006. Effect of temperature and detergents on agrobacterium tumefaciens, the causal pathogen of crown gall disease of walnut. American Phytopathological Society Abstracts.Interpretive Summary:
                                Technical Abstract: Crown gall disease caused by the bacterium Agrobacterium tumefaciens causes significant economic losses in commercial walnut orchards and nursery operations in California.  In an effort to develop integrated control strategies to ensure pathogen and disease free plant material at nurseries, the effects of detergents and temperature on growth and survival of Agrobacterium tumefaciens were investigated.  At temperatures above 37 degrees C and below 15 degrees C in-vitro bacterial multiplication rates were significantly repressed.  A. tumefaciens did not survive above 45 degrees C.  Exposure to a range of concentrations of cationic and non-ionic detergents significantly reduced bacterial survival.  Exposure to the anionic detergent SDS, at 0.1% and 1% concentrations, showed limited effect on bacterial viability.  Among the detergents tested, cetyltrimethylammonium bromide (CTAB) was the most effective reducing bacterial populations below detection limits at concentrations above 0.01% (V/V).  Data on the synergistic effect of combining detergent exposure with 1 to 3 freeze-thaw cycles also will be presented.  The use of detergent washes combined with manipulation of pre-plant seedling storage temperatures may reduce the incidence of crown gall on walnut under orchard conditions.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov