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Title:   Author  item
                                            Wise, Rogeritem
                                            SCHULZE-LEFERT, PAUL - SAINSBURY LABORATORYitem
                                            ZHOU, FASONG - SAINSBURY LABORATORYitem
                                            KURTH, JOACHIM - SAINSBURY LABORATORYitem
                                            Halterman, Dennisitem
                                            WEI, FUSHENG - IOWA STATE UNIVERSITYitem
                                            ELLIOT, CANDACE - SAINSBURY LABORATORY
  Submitted to: Patent Application
                                    Publication Type: Patent Application
                                    Publication Acceptance Date: 5/28/2002
                                    Publication  Date: 5/28/2002
                                    Citation: Wise, R.P., Schulze-Lefert, P., Zhou, F., Kurth, J., Halterman, D.A., Wei, F., Elliot, C. 2002. Resistance gene. Patent Application. U.S. Patent 10/148,351.Interpretive Summary:
                                Technical Abstract: The barley Mla locus confers multiple resistance specificities to the obligate fungal biotroph, Blumeria (=Erysiphe) graminis f. sp. hordei.  Interspersed within the 240-kb Mla complex are three families of resistance gene homologs (RGHs).  Probes from the Mla-RGH1 family were used to identify three classes of cDNAs.  The first class is predicted to encode a full-length CC-NBS-LRR protein and the other two classes contain alternatively spliced, truncated variants.  Utilizing a cosmid that contains a gene corresponding to the full-length candidate cDNA, a single-cell expression assay was used to demonstrate complementation of AvrMla6-dependent resistance to B. graminis in barley and wheat.  This assay was also used to substantiate previous genetic data that the Mla6 allele requires the signaling pathway component, Rar1, for function.  Computational analysis of Mla6 and the Rar1-independent, Mla1 protein reveals 91.2% sequence identity and shows that the LRR domain is subject to diversifying selection.  Our findings demonstrate that highly related CC-NBS-LRR proteins encoded by alleles of the Mla locus can dictate similar powdery mildew resistance phenotypes yet still require distinct downstream signaling components.


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