U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Temperate Tree Fruit and Vegetable Research: Wapato, WA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Facilities
                        USDA Laboratory in Wapato, WA
                                    USDA Prosser Worksite
                                    USDA Moxee Farm
                    About UsSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Pacific West Area Wapato, Washington Temperate Tree Fruit and Vegetable Research Research





- Swisher Grimm, Kylie
- Plant Genetic Resources, Genomics and Genetic Improvement (301)

Research Project: **Location:
                            Temperate Tree Fruit and Vegetable Research** Project Number: 2092-21220-003-020-T
                        
                        Project Type: Trust Fund Cooperative Agreement Start Date: May 1, 2022
                        
                        End Date: Jun 30, 2025 **Objective:**
                        
                        Develop segregating populations with combined resistances to Columbia root-knot nematode (CRKN) and Potato virus Y (PVY), and identify germplasm with combined resistance to CRKN and PVY using greenhouse and molecular marker screening. **Approach:**
                        
                        1. Development of segregating populations with combined resistances to CRKN and PVY 
Currently, all the CRKN germplasm that was developed by Tri-state breeding program are male sterile and can be used only as female parent in the hybridizations. Similarly, most of the germplasm and the varieties resistant to PVY are also male sterile except for PVY resistance from S. chacoense  and some germplasm from S. andigenum. In this objective we plan on making controlled crosses with resistance to CRKN as female parent (PA99N82-4, POR15NCKY022-1) and use male fertile PVY resistant clones (Saikai 35, DG91-31, POR00LB6-1, AOR13260-3adg) as male parents. We plan on generating 4,000 to 5000 seedlings from these crosses. These populations will segregate for resistances to both CRKN and PVY.
2. Identification of germplasm with combined resistance to CRKN and PVY using greenhouse and molecular marker screening
All the seedlings segregating for resistance to CRKN and PVY will be initially screened for presence of molecular markers linked to CRKN and PVY. We expect 50 % of the seedlings to be individually resistant to CRKN and PVY and at least 10 % of seedlings with resistance to both pathogens. The seedlings that have markers linked to both CRKN and PVY will be identified and will be confirmed for their resistance to both the pathogens using greenhouse screening. Screening will consist of inoculations of CRKN and mechanical inoculations with PVY. Confirmed clones with resistance to both the diseases will either be entered into the Oregon variety development program (those with agronomic merit) or will be used as parental clones for future breeding and variety development.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov