U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Food and Feed Safety Research: College Station, TX
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Plains Area College Station, Texas Southern Plains Agricultural Research Center Food and Feed Safety Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Genovese, Kenneth

                                                         - *Ken*item
                                            He, Louisitem
                                            LOWRY, VIRGINIA - TX A&M UNIVERSITYitem
                                            Nisbet, Daviditem
                                            Kogut, Michael

                                                         - *Mike*
  Submitted to: FEMS Immunology and Medical Microbiology
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 5/17/2007
                                    Publication  Date: 7/19/2007
                                    Citation: Genovese, K.J., He, H., Lowry, V.K., Nisbet, D.J., Kogut, M.H. 2007. Dynamics of the avian inflammatory response to Salmonella following administration of the toll-like receptor 5 agonist flagellin. FEMS Immunology and Medical Microbiology. 51(1):112-117.Interpretive Summary: This study describes the use of a bacterial-derived protein, flagellin, as an immune-stimulatory agent in chickens.  Chickens were given the flagellin as an injection into their abdomen.  One hour later, chickens were then given an abdominal injection of Salmonella bacteria.  Following the injection of Salmonella, blood was collected at different time points and the number and types of white blood cells were counted.  Cells moving into the abdomen were also counted and typed.  In addition, mortality was compared between flagellin-treated and untreated chickens after an abdominal injection of Salmonella bacteria.  The results of these studies indicate that flagellin caused an increase in white blood cells in the circulating blood supply and in the abdomen.  The primary cell accounting for the increase in white blood cells both in the abdomen and in the circulating blood was the heterophil.  Flagellin injection also reduced mortality associated with Salmonella injection by 50%.  In conclusion, flagellin stimulates the chicken's immune system and in doing so, protects chickens from Salmonella-associated mortality.
                                Technical Abstract: Flagellin is a highly evolutionarily conserved bacterial component that is recognized by the innate immune system through toll-like receptor (TLR) 5.  Previous work has shown that flagellin is a potent stimulator in vitro of phagocytic cell functions of chickens.  The purpose of the present study was to define the effects of flagellin administration on the avian inflammatory response to Salmonella in chickens.  Chicks administered an intra-abdominal (IA) injection of flagellin had significantly (P less than or equal to 0.05) increased peripheral white blood cells (PWBC) compared to saline-injected control birds and Salmonella-injected birds at 4, 8, and 24hr post-injection (P less than or equal to 0.05).  In addition, IA white blood cell infiltrates (IWBC) were significantly higher in the flagellin-injected birds compared to saline- and Salmonella-injected birds (P less than or equal to 0.05).  In both the peripheral blood and in the abdominal cavity, the predominant leukocyte accounting for the increases observed in cell numbers was the heterophil (P less than or equal to 0.05).  IA injection of flagellin was also found to reduce Salmonella-associated mortality by 50% compared to mortality observed in the Salmonella-injected control groups (P less than or equal to 0.05). The data suggest that the TLR 5 agonist, flagellin, is also a potent stimulator of a heterophil-mediated innate immune response in vivo, with subsequent protective induction of an inflammatory response to bacterial infections in the chicken.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov