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Title:   Author  item
                                            Miles, Jeremyitem
                                            Vallet, Jeff
  Submitted to: Midwestern Section of the American Society of Animal Science
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 11/27/2006
                                    Publication  Date: 7/20/2007
                                    Citation: Miles, J., Vallet, J. 2007. Characterization of mRNA expression for the endothelial differentiation G-protein-coupled (EDG) receptors in porcine endometrial tissue during the pre-implantation period of pregnancy [abstract]. Journal of Animal Science. 85 (Supplement 2):117-118. (Abstract #235)Interpretive Summary:
                                Technical Abstract: Establishment of pregnancy in the pig requires proper preparation of the uterine environment. Although the mechanisms by which the endometrium undergoes preparation for embryo implantation are not clear, recent evidence has suggested that lysophospholipids, such as sphingosine 1-phosphate (S1P) and lysophosphatidic acid (LPA) which function through the endothelial differentiation G-protein-coupled (EDG) receptors may play a role in coordinating uterine preparation for embryo implantation. The objective of the current study was to characterize mRNA expression for the EDG receptors in porcine endometrial tissue during the pre-implantation period of pregnancy. Total RNA was isolated from endometrial tissue at d 10, 12, 14 and 16 of gestation (n = 4 gilts per day of gestation) and mRNA expression for the EDG receptors was measured via real-time PCR. Data were analyzed for analysis of variance using GLM procedures. At all days of gestation examined, mRNA for four of the five high-affinity EDG receptors for S1P (EDG1, EDG3, EDG5 and EDG8) were detected by RT-PCR, while EDG6 expression was not detected. Expression of EDG1 mRNA was increased (P < 0.01) in endometrial tissue at d 14 and 16 of gestation compared with d 10 and 12. In contrast, the expression of EDG5 mRNA was increased (P < 0.01) in endometrial tissue at d 10 and 12 compared with d 14 and 16. Expression of mRNA for all three high-affinity EDG receptors for LPA (EDG2, EDG4 and EDG7) were detected by RT-PCR at all days of gestation examined. Expression of mRNA for EDG7 was increased (P < 0.01) in endometrial tissue at d 12 of gestation compared with endometrial tissue at d 10, 14 and 16 of gestation. These results demonstrate altered gene expression for the EDG receptors in porcine endometrial tissue throughout the pre-implantation period of pregnancy, suggesting that S1P and LPA may play a role in the preparation of the uterine environment for embryo implantation in the pig.


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