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Title:   Author  item
                                            MA, WENJUN - IOWA STATE UNIVERSITYitem
                                            Baker, Amyitem
                                            GRAMER, MARIE - UNIVERSITY OF MINNESOTAitem
                                            BROCKWELL, CHRISTY - UNIVERSITY OF TENNESSEEitem
                                            JANKE, BRUCE - IOWA STATE UNIVERSITYitem
                                            Lager, Kellyitem
                                            Gauger, Phillipitem
                                            WEBBY, RICHARD - ST JUDE CHILDRENS RESEARCitem
                                            Richt, Juergen
  Submitted to: American Association of Veterinary Laboratory Diagnosticians
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 7/15/2007
                                    Publication  Date: 10/18/2007
                                    Citation: Ma, W., Vincent, A., Gramer, M., Brockwell, C., Janke, B., Lager, K., Gauger, P., Webby, R., Richt, J. 2007. Characterization of the novel H2N3 influenza virus subtype isolated from U.S. pigs [abstract]. American Association of Veterinary Laboratory Diagnosticians 50th Annual Conference. p. 123.Interpretive Summary:
                                Technical Abstract: Pigs have been suggested to be the mixing vessel for avian and human influenza viruses because the porcine trachea contains binding receptors with preferences for human and avian influenza viruses. In the pig, genetic reassortment to create novel influenza subtypes by mixing avian, human and swine influenza viruses is possible. Here, we report the characterization of two novel reassortant H2N3 viruses isolated from pigs with respiratory disease.  Molecular and phylogenetic analyses of these viruses revealed that the HA segment is similar to an avian influenza virus (AIV) H2N3 isolated from mallards and the NA sequence is similar to an AIV H4N3 isolated from blue-winged teal. The HA protein revealed a Q226L mutation when compared with the putative parental avian HA protein, indicating a preferential binding to SA'2,6Gal, the mammalian influenza receptor.  All internal genes except PA were similar to influenza virus gene segments found in contemporary triple reassortant (human, swine, avian) SIVs in the United States.  The PA segment has high homology to the PA of an AIV H6N5 isolated from mallards.  Experimental infection of swine showed that the H2N3 virus is virulent for pigs, replicating in the lung and causing macroscopic and microscopic lung lesions.  All experimentally inoculated pigs seroconverted to H2N3 and seroconversion was demonstrated in sentinel contact pigs as well.  In addition, the H2N3 virus infected mice, inducing lung lesions, clinical disease and death.  The H2N3 virus also infected ferrets and transmitted efficiently to contact sentinel ferrets.  These data indicate that the novel reassortant H2N3 subtype virus has the ability to infect various mammalian hosts.


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