U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 close



Research
                Research Home
                            National Programs
                            Research Projects
                            Scientific Manuscripts
                            International Engagement
                            Scientific Software/Models
                            Databases and Datasets
                            Office of Scientific Quality Review
            Media
                News & Features
                            Multimedia
                            Briefing Room
                            Down on the Farm
                            Press Room
                            Science in Your Shopping Cart
                            Scientific Discoveries
                            Tellus
                            Under the Microscope
            About ARS
                About ARS Home
                            Staff Directory
                            Labs and Research Centers (Map)
                            Headquarter Offices
                            Organizational Chart
                            Employee Services (REE Employees Only)
                            Office of Outreach, Diversity, and Equal Opportunity
            Work With Us
                Work With Us Home
                            ARS Vacancies at USAJOBS
                            Careers at ARS Information
                            Scientific Collaborations
                            Extramural Agreements
                            1890 Faculty Research Sabbatical ProgramSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            DALLOUL, RAMI - USDA ARS BELTSVILLE MDitem
                                            Lillehoj, Hyunitem
                                            KLINMAN, DENNIS - FDA BETHESDA MDitem
                                            DING, XICHENG - USDA ARS BELTSVILLE MDitem
                                            MIN, WONGI - SUNCHON NAT U, KOREAitem
                                            Heckert, Robertitem
                                            LILLEHOJ, ERIK - U MD BALTIMORE MD
  Submitted to: Annual Conference on Vaccine Research
                                    Publication Type: Proceedings
                                    Publication Acceptance Date: 3/26/2005
                                    Publication  Date: 5/5/2005
                                    Citation: Dalloul, R.A., Lillehoj, H.S., Klinman, D.M., Ding, X., Min, W., Heckert, R.A., Lillehoj, E.P. 2005. Cpg oligodeoxynucleotides co-administered with the microneme protein mic2 protect against eimeria infections.. Annual Conference on Vaccine Research 64.Interpretive Summary: Coccidiosis is caused by several different Eimeria parasites which infect the intestine of susceptible host.  Avian coccidiosis causes serious economic losses to US poultry industry and there is a need for the development non-drug-dependant control strategy against this infection.  In this communication, ARS scientists, in collaboration with scientists at University of Maryland and US Food and Drug Administration have discovered new oligopeptide immunomodulators which enhance gut immunity in chickens.  These scientists have identified new short synthetic oligodeoxynucleotide sequences containing unmethylated CpG motifs (CpG ODNs) which stimulate chicken innate immunity and enhance disease resistance against avian coccidiosis.  The results of this study will provide basic groundwork for utilization of ODNs as immunomodulators for poultry disease control.
                                Technical Abstract: Short synthetic oligodeoxynucleotides containing unmethylated CpG motifs (CpG ODNs) were previously shown to exert a positive effect on weight loss and oocyst shedding associated with Eimeria infection when injected in vivo.  The present work investigated the effects of in ovo vaccination with CpG ODNs and an Eimeria recombinant microneme protein (MIC2), alone or in combination, on chicken susceptibility to coccidiosis.  In ovo injection of CpG ODNs alone enhanced resistance to experimental E. acervulina infection as best exemplified by reduced oocyst shedding.  Two CpG ODNs reduced the oocyst load, but did not affect weight gain.  When co-administered with the recombinant microneme protein, both ODNs reduced oocyst shedding; however, only ODN D19 plus MIC2 consistently improved weight gain.  Vaccinating with ODN 2006 or MIC2 protein curtailed oocyst shedding but did not enhance weight gain in E. tenella-infected birds.  Co-administration of CpG ODN and MIC2 did not have an additive effect in reducing the oocyst output; however, it resulted in the highest and lowest Ab response before and after E. tenella infection, respectively.  Collectively, CpG ODNs administered in ovo demonstrated immunoenhancing and adjuvant effects following Eimeria infections.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov