U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Vegetable Research: Charleston, SC
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Cucurbit Genetics CooperativeSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Southeast Area Charleston, South Carolina Vegetable Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Wechter, William

                                                         - *Pat*item
                                            Kluepfel, Daniel
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 7/30/2005
                                    Publication  Date: 7/30/2005
                                    Citation: Wechter, W.P., Kluepfel, D.A. 2005. Global gene expression of Pseudomonas Putida KT2440 during growth in the rhizosphere of Arabidopsis Thaliana [abstract]. Phytopathology 95 (6):S110.Interpretive Summary:
                                Technical Abstract: Identification of the bacterial genes involved, and the functions they provide, in colonization of the rhizosphere is crucial to a comprehensive understanding of the interaction of plants with both beneficial and pathogenic microorganisms.  Here we report on the utilization of full-genome microarrays to perform gene expression studies of Pseudomonas putida KT2440 in the rhizosphere of Arabidopsis thaliana Columbia.  The Arabidopsis rhizosphere, which was supported on a minimal medium agar, was inoculated with KT2440 and then sampled at 48 hr and 120 hr post-inoculation.  RNA was extracted from bacteria growing in the rhizosphere as well as from bacteria growing in the absence of plant roots and used to hybridized to P. putida KT2440 microarrays.  At 48 hr post-inoculation we identified 26 bacterial genes that were induced and 149 genes that were repressed in the rhizosphere at a level of 2-fold or greater.  In addition, 195 bacterial genes were modulated as a function of growth in the rhizosphere 120 hr post-inoculation.  Thirty-one percent of the genes up-regulated after 48 hr returned to basel expression levels 72 hr later while 85 unique genes were up-regulated only at the 120 hr sample time.  The identity of these rhizosphere-modulated bacterial genes and their potential involvement in root colonization will be discussed.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov