U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Infectious Bacterial Diseases Research: Ames, IA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area Ames, Iowa National Animal Disease Center Infectious Bacterial Diseases Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Bannantine, Johnitem
                                            Waters, Wadeitem
                                            Palmer, Mitchellitem
                                            KAPUR, VIVEK - UNIVERSITY OF MNitem
                                            Stabel, Judithitem
                                            Paustian, Michael
  Submitted to: International Colloquium on Paratuberculosis
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 8/13/2005
                                    Publication  Date: 7/1/2006
                                    Citation: Bannantine, J.P., Waters, W.R., Palmer, M.V., Kapur, V., Stabel, J.R., Paustian, M. 2006. Development and use of a mycobacterium avium subsp. paratuberculosis partial protein array for discovery of novel antigens [abstract]. International Colloquium on Paratuberculosis. p. 146.Interpretive Summary:
                                Technical Abstract: With the genome sequence available for Mycobacterium avium subspecies paratuberculosis (MAP), the technology is now developed for protein arrays to detect the presence of antibodies directed against MAP in host serum.  The power of this approach is that it enables a direct comparison of MAP proteins to each other in relation to their immunostimulatory capabilities.  In this study, over 60 MAP coding sequences were heterologously expressed and purified for use in a partial protein array.  MAP proteins represented on this array include unknown hypothetical, cell surface, and MAP-specific proteins as well as previously characterized or known MAP antigens.  The array was exposed to sera from MAP immunized rabbits and mice to identify immunodominant antigens in those artificial conditions.  Furthermore, sera from non- and MAP-infected cattle were used to probe the array to identify antigens in the context of disease.    Distinct sets of antigens emerged when data were compared between Johne’s disease animals versus that of immunized mice and rabbits, indicating that immunodominant antigens in those artificial hosts may not represent antigens detected in the context of disease.  Ten of the 64 proteins bound significant levels of antibody from clinical cattle.  Of these ten proteins, three were previously identified as MAP antigens and the remaining seven represent novel antigens.  Sera from three experimentally infected cattle identified at least one putative surface antigen detected early in infection.  These data suggest this antigen may be useful in the early diagnosis of MAP infections.  This powerful combination of genomic information, molecular tools, and immunological assays has enabled the identification of previously unknown antigens of MAP.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov