U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Food and Feed Safety Research: College Station, TX
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Plains Area College Station, Texas Southern Plains Agricultural Research Center Food and Feed Safety Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Genovese, Kenneth

                                                         - *Ken*item
                                            He, Louisitem
                                            LOWRY, VIRGINIA - TX A&M UNIVERSITYitem
                                            Swaggerty, Christina

                                                         - *Christi*item
                                            Nisbet, Daviditem
                                            Kogut, Michael

                                                         - *Mike*
  Submitted to: Congress International Society Develop Comparative Immunology
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 2/27/2006
                                    Publication  Date: 7/1/2006
                                    Citation: Genovese, K.J., He, H., Lowry, V.K., Swaggerty, C.L., Nisbet, D.J., Kogut, M.H. 2006. Cell signaling and host innate immune defenses in wild-type and commercial turkeys [abstract]. 10th International Congress of Developmental and Comparative Immunology. p. 110.Interpretive Summary:
                                Technical Abstract: The purpose of the present study was to measure any functional differences in heterophils isolated from a commercial line (A) to wild-type Rio Grande turkeys and to observe differences in intracellular signaling.  Mitogen-activated protein kinases (MAPK) p38 and ERK 1/2 and total protein tyrosine kinase (PTK) activities were measured.  In signaling assays, after stimulation with Salmonella (SE) or opsonized SE (OPSE), cells were lysed and lysates were tested for MAPK and PTK activity using commercial ELISA kits.  On both day 4 and 7 post-hatch, Rio Grande heterophils had significantly higher levels of ERK 1/2 and p38 MAPK kinase activity upon stimulation with SE and OPSE.  PTK values on day 4 and 7 post-hatch in Rio Grande heterophils was significantly higher upon stimulation with SE than with OPSE and was significantly higher than the PTK levels in Line A upon SE and OPSE stimulation.  Heterophil phagocytosis of SE, oxidative burst (OXB) and degranulation (DGR) were assayed.  On days 4 and 7 post-hatch, Rio Grande heterophils responded to phorbol A-myristate 13 acetate (PMA) with significantly greater OXB activity than Line A. The DGR assay also revealed a significantly greater level of activity in Rio Grande heterophils compared to heterophils from Line A. No differences in the phagocytosis of SE were observed between lines.  These results demonstrate that wild-type Rio Grande turkeys possess greater innate immune functions than their commercial counterparts and suggest that improvements in the innate system of commercial birds early in life could be beneficial in the prevention of disease.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov