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Title:   Author  item
                                            HUMPHREY, BROOKE - UNIV OF CALIF DAVISitem
                                            Stephensen, Charlesitem
                                            CALVERT, CHRIS - UNIV OF CALIF DAVISitem
                                            KLASING, KIRK - UNIV OF CALIF DAVIS
  Submitted to: Comparative Biochemistry and Physiology
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 6/23/2004
                                    Publication  Date: 6/16/2004
                                    Citation: Humphrey, B.D., Stephensen, C.B., Calvert, C.C., Klasing, K.C. 2004. GLUCOSE AND CATIONIC AMINO ACID TRANSPORTER EXPRESSION IN GROWING CHICKENS (GALLUS GALLUS DOMESTICUS). Comparative Biochemistry and Physiology. 138:515-525.Interpretive Summary: Tissue glucose transporter (GLUT1-3) and cationic amino acid 
transporter (CAT1-3) mRNA expression was determined in growing broiler chicks posthatch.  In two experiments, tissues were either collected on d 1, 3 and 7 or d 1 and 14 posthatch.  Heart and liver were the only tissues expressing a GLUT isoform on d 1.  All tissues expressed a GLUT isoform on d 7 except for the thymus.  Most tissues expressing a CAT isoform on d 1 decreased mRNA levels through d 7 (P<0.05), except for bursa CAT-1 which tended to increase (P=0.05).  The thymus and spleen did not express any CAT isoform mRNA until d 7. The liver was the only tissues expressing GLUT-2 mRNA through d 14. On d 14, GLUT-1, CAT-1 and CAT-2 mRNA were differentially expressed across tissues (P<0.05).  High affinity GLUT and CAT mRNA expression was highest in the heart and bursa, respectively (P<0.05). Total CAT mRNA expression was greatest in the bursa P<0.05). The thymus had the lowest high affinity GLUT and total CAT mRNA expression on d 14 posthatch.  Therefore, T lymphocytes within the thymus may be most susceptible to glucose and cationic amino acid supply.
                                Technical Abstract: Tissue glucose transporter (GLUT1-3) and cationic amino acid 
transporter (CAT1-3) mRNA expression was determined in growing broiler chicks posthatch.  In two experiments, tissues were either collected on d 1, 3 and 7 or d 1 and 14 posthatch.  Heart and liver were the only tissues expressing a GLUT isoform on d 1.  All tissues expressed a GLUT isoform on d 7 except for the thymus.  Most tissues expressing a CAT isoform on d 1 decreased mRNA levels through d 7 (P<0.05), except for bursa CAT-1 which tended to increase (P=0.05).  The thymus and spleen did not express any CAT isoform mRNA until d 7.  The liver was the only tissues expressing GLUT-2 mRNA through d 14.  On d 14, GLUT-1, CAT-1 and CAT-2 mRNA were differentially expressed across tissues (P<0.05).  High affinity GLUT and CAT mRNA expression was highest in the heart and bursa, respectively P<0.05).  Total CAT mRNA expression was greatest in the bursa (P<0.05).  The thymus had the lowest high affinity GLUT and total CAT mRNA expression on d 14 posthatch.  Therefore, T lymphocytes within the thymus may be most susceptible to glucose and cationic amino acid supply.


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