U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Western Human Nutrition Research Center: Davis, CA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Laboratories
                        Allen Group
                                    Bennett Group
                                    Stephensen Group
                                    Human Studies
                                    Metabolic Kitchen
                                    Huang Group
                                    Kable Group
                                    Keim Group
                                    Laugero Group
                                    Newman Group
                                    Lemay Group
                                    Support Staff
                    About Us
                        HistorySearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Pacific West Area Davis, California Western Human Nutrition Research Center Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications
- Charles Stephensen
- Charles Stephensen
- Brian Bennett
- Ellen Bonnel
- Yuriko Adkins
- Lindsay Allen
- Bess Caswell
- Liping Huang
- Mary Kable
- Nancy Keim
- Kevin Laugero
- Danielle Lemay
- John Newman
- Ryan Snodgrass
- Zeynep Alkan
- Allyson Deimeke
- Rashel (Shelly) DeCant
- Nicholas Esparza
- Steven Nguyen
- Erik Gertz
- Daniela Hampel
- Shurong Huang
- Xiaowen Jiang
- James Gomez
- Setti Shahab-Ferdows
- Debra Standridge
- Dave Storms

Title:   Author  item
                                            Stephensen, Charlesitem
                                            JIANG, XIAOWEN - UNIV OF CALIF DAVISitem
                                            FREYTAG, TAMMY - UNIV OF CALIF DAVIS
  Submitted to: Journal of Nutrition
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 7/1/2004
                                    Publication  Date: 10/1/2004
                                    Citation: Stephensen, C.B., Jiang, X., Freytag, T. 2004. Vitamin a deficiency increases the in vivo development of il-10-positive th2 and decreases development of th1 cells in mice. Journal of Nutrition. 134:2660-2666.Interpretive Summary:
                                Technical Abstract: Vitamin A deficiency impairs both Th1- and Th2-mediated immune responses. Multiple mechanisms are involved but the hypothesis that deficiency affects development of Th1/Th2 memory cell phenotype, has not been directly tested in vivo. To do so, lymphocytes from DO11.10 TCR-transgenic mice were transferred to vitamin A-deficient or control BALB/c recipients. Recipients were then immunized with the cognate peptide antigen for the TCR-transgenic DO11.10 T-cells (OVA323-339). Two to five weeks later the transferred OVA323-339-specific T-cells were identified from draining lymph nodes with the CRclonotypic antibody KJ1-26 and their Th1/Th2 phenotype was characterized by intracellular cytokine staining following in vitro stimulation with phorbol myristate acetate and ionomycin. The percentage of CD4+KJ1-26+ cells positive for IL-10 was two-fold greater in vitamin A-deficient mice (3.49 +/-0.41%; mean standard error) than in control mice (1.74 +/-0.37%). No difference was seen for IL-4. In addition, the percentages of CD4+KJ1-26+ cells from vitamin A-deficient mice that were positive for IFN-10 (8.8 +/-0.73%) and IL-2 (39.5 +/-3.1%) were both lower than the percentages seen in control mice (11.4 +/-0.67% and 47.0 +/-2.8%, respectively). Thus vitamin A deficiency at the time of initial antigen exposure enhances the development of IL-10-producing Th2 or Treg cells and diminishes the development of Th1 memory cells. This work was supported by USDA NRI grant # 97-35200-4229, USDA CRIS Project # 5306-51530-006-00D and NIH grant # 1 R01 AI 0863.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov