U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Food and Feed Safety Research: College Station, TX
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Plains Area College Station, Texas Southern Plains Agricultural Research Center Food and Feed Safety Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            DIMITRIJEVIC, MIRJANA - UNIV OF BELGRADEitem
                                            Anderson, Robinitem
                                            Callaway, Todditem
                                            JUNG, YONG - 6202-40-30item
                                            Harvey, Rogeritem
                                            RICKE, STEVEN - TX A&M UNIVERSITYitem
                                            Nisbet, David
  Submitted to: Journal of Food Protection
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 12/22/2005
                                    Publication  Date: 5/1/2006
                                    Citation: Dimitrijevic, M., Anderson, R.C., Callaway, T.R., Jung, Y.S., Harvey, R.B., Ricke, S.C., Nisbet, D.J. 2006. Inhibitory effects of select nitrocompounds on growth and survivability of Listeria monocytogenes in vitro. Journal of Food Protection. 69:1061-1065.Interpretive Summary: Listeria monocytogenes is an important pathogen capable of causing invasive infections in animals and humans.  It is estimated that L. monocytogenes causes approximately 2500 serious human illnesses and as many as 500 deaths per year in the United States at substantial cost.  We report here results from experiments testing the effects of the potential chemical inhibitors 2-nitro-1-propanol (2NPOH), 2-nitroethanol (2NEOH) and nitroethane (NE), on growth and survivability of L. monocytogenes.  In all cases, inhibition was greatest with 2NPOH and least with NE.  For instance, the growth of L. monocytogenes was significantly reduced by as much as 78% when tested with 2NPOH.  Inhibition of L. monocytogenes growth by 2NPOH, 2NEOH and NE was observed whether tested at two typical incubation temperatures, 30 and 37 deg C, or whether in the presence of oxygen, as would be expected in feed or food, or in the absence of oxygen, as would be expected in gut environments.  The survivability of L. monocytogenes was also reduced during storage at refrigeration temperatures.  The ability of L. monocytogenes to survive and grow at cold temperatures is one of the characteristics that make it such a dangerous pathogen.  Results from the present study show that 2NEOH, NE and in particular, 2NPOH, inhibited L. monocytogenes under a variety of conditions.  This research provides the basis for further studies to develop new disinfectants and feed ingredients to control this important foodborne pathogen in animals and foods.  Ultimately, this research will help farmers and food manufacturers produce safer food products for the American consumer.
                                Technical Abstract: We report the effects of 2-nitro-1-propanol (2NPOH), 2-nitroethanol (2NEOH) and nitroethane (NE), on growth and survivability of L. monocytogenes.  In all cases, inhibition was greatest with 2NPOH and least with NE.  For instance, specific growth rates of L. monocytogenes strain 18 were reduced (P < 0.05) 76, 60 and 29% from controls incubated without added nitrocompound (0.62 ± 0.02 h**-1) during aerobic culture at 37 deg C in brain heart infusion (BHI) broth containing 10 mM 2NPOH, 2NEOH or NE, respectively.  Specific growth rates of L. monocytogenes strain Scott A were reduced (P < 0.05) 67, 45 and 11%, respectively, from controls (0.67 ± 0.02 h**-1) when cultured similarly.  Specific growth rates for L. monocytogenes strain 18 incubated similarly except at 30 deg C were reduced (P < 0.05) 76, 60 and 30%, respectively, and were reduced (P < 0.05) 78, 23 and 23% during anaerobic culture at 30 deg C in BHI containing 15 mM 2NPOH, 2NEOH or NE (control rates ranged from 0.37 ± 0.07 to 0.74 ± 0.05 h**-1).  Survivability of L. monocytogenes strain 18 was reduced (P < 0.05) during aerobic storage (4 mo at 4 deg C) in BHI containing 2NPOH or 2NEOH (by 7.8 and 1.9 log units, respectively) but not NE.  The inhibitory effect of 2NPOH was approximately 20% greater during growth at pH 7.0 than at pH 5.6 or 8.0. These results demonstrate the differential inhibitory activity of 2NPOH, 2NEOH and NE against L. monocytogenes in vitro.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov