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Title:   Author  item
                                            Lillehoj, Hyunitem
                                            Min, Wongi
  Submitted to: Avian Immunology Group Meeting
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 4/30/2004
                                    Publication  Date: 5/1/2004
                                    Citation: Lillehoj, H.S., Min, W. 2004. High-throughput gene expression analysis using t lymphocyte cdna microarray to identify eimeria-induced host genes mediating cell-mediated immunity against coccidosis.. Avian Immunology Group Meeting, p. 22.Interpretive Summary:
                                Technical Abstract: Avian coccidiosis is caused by several Eimeria strains which infect different regions of the intestine inducing a strain-specific immunity. Coccidiosis usually stimulate a number of immunological defense mechanisms, namely both antibody- and cell-mediated. Recent technological advances in high-throughput molecular approaches to identify disease resistance genes and molecular/cell biological pathways associated with complex biological phenomenon now enable an alternative strategy to combat coccidiosis. Our recent studies on local host immunity to Eimeria clearly indicate that intricate and complex interactions of host local cell-mediated immunity and parasites determine the outcome of host response to coccidiosis. High-throughput gene expression profiling using 400 immunity genes and real-time PCR have been applied to analyze underlying immune mechanisms controlling disease resistance/susceptibility to coccidiosis. The results indicate role of various cytokines in controlling host intestinal innate immune response to Eimeria (This study was supported in part by a Cooperative State Research Service, NRI grant under agreement number 2003-35205-12838).


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