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 Temperate Tree Fruit and Vegetable Research: Wapato, WA
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Title:   Author  item
                                            BARCENAS, NINA - WA ST UNIV,DEPT ENTOMOLOGitem
                                            Unruh, Thomasitem
                                            Neven, Lisa
  Submitted to: Western Orchard Pest and Disease Management Conference
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 12/10/2003
                                    Publication  Date: 1/14/2004
                                    Citation: Barcenas, N., Unruh, T.R., Neven, L.G. 2004. DNA-based diagnostics for Lepidopteran pests of pome fruits. Western Orchard Pest and Disease Management Conference. p.35.Interpretive Summary: Abstract only; please refer to technical abstract section
                                Technical Abstract: CO-1 mitochondrial DNA from the 4 tortricids which feed internally in apples and pears in the USA was sequenced and compared.  Primer sets were designed that selectively amplify each species and protocols were developed for a real-time PCR instrument and for traditional PCR followed by agarose electrophoresis.  Cydia pomonella and the 3 Grapholita species (G. molesta, G. packardi, G. prunivora) are readily separated. The technique is robust for geographically disparate populations of each species. Mexico is now using the method to confirm identifications of larvae found in intercepted fruit.  A possible new species of Grapholita, near packardi was seen attacking rose hips in Washington.


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