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Title:   Author  item
                                            LEAKEY, ANDREW D - UNIVERSITY OF ILLINOISitem
                                            XU, FANGXIU - UNIVERSITY OF ILLINOISitem
                                            GILLESPIE, KELLY - UNIVERSITY OF ILLINOISitem
                                            HEADY, LINDSEY - BROOKHAVEN NATIONAL LABitem
                                            QUI, QUANSHENG - PHOTOSYNTHESIS RES UNITitem
                                            RUPASSARA, S. INDU - UNIVERSITY OF ILLINOISitem
                                            Ainsworth, Elizabeth

                                                         - *Lisa*item
                                            BOHNERT, HANS - UNIVERSITY OF ILLINOISitem
                                            Huber, Stevenitem
                                            LONG, STEPHEN - UNIVERISTY OF ILLINOISitem
                                            ROGERS, ALISTAIR - BROOKHAVEN NATIONAL LABitem
                                            Ort, Donald
  Submitted to: American Society of Plant Biologists
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 3/1/2006
                                    Publication  Date: 6/1/2006
                                    Citation: Leakey, A.D.B., Xu, F., Gillespie, K.M., Heady, L., Qiu, Q., Rupassara, S., Ainsworth, E.A., Bohnert, H.J., Huber, S.C., Long, S.P., Rogers, A., Ort, D.R. 2006. How will the gene expression profile, biochemistry and physiology of soybean leaves respond to growth at elevated [CO2] under open-air field conditions? American Society of Plant Biologists Abstracts.  Available: http://abstracts.aspb.org/pb2006/public/P01/P01013.html.Interpretive Summary:
                                Technical Abstract: The atmospheric concentration of carbon dioxide [CO2] is projected to increase from 380 ppm today to 550 ppm in 2050, with significant consequences for plant function in both natural and managed ecosystems. Many studies have investigated the physiological responses of plants to growth at elevated [CO2]. Despite this large body of knowledge, we cannot explain how the effects of elevated [CO2] vary with other aspects of the abiotic environment, such as temperature, water stress and ozone concentration. Data are particularly lacking for plants grown under field conditions where these interactions play an important role. To gain further understanding of plant responses to elevated [CO2] we are combining genomic, metabolomic, biochemical and physiological investigation of soybean grown in the field at the SOYbean Free-Air Concentration Enrichment (SOYFACE) facility at the University of Illinois. Soybean was grown in four plots at ambient [CO2] (~380 ppm) and four plots at elevated [CO2] (~550 ppm), from sowing until harvest, in 2005. This provides a model system, where low genetic and environmental variability between experimental units increases the ability of the experiment to detect subtle treatment effects. On 6 dates during reproductive development, photosynthetic gas exchange was measured at midday on the youngest, fully expanded leaf. Immediately afterwards, leaf tissue was frozen and stored in liquid N. Microarray analysis of the gene expression profile was conducted using 72 Affymetrix Soybean Gene Chips. These data are presented alongside measures of leaf metabolomics, enzyme activity, water, carbohydrate, nitrogen, protein, amino acid, anti-oxidants and protein oxidation. This will provide a novel, integrated analysis of responses to growth at elevated [CO2].


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