U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Plant Science Research: St. Paul, MN
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area St. Paul, Minnesota Plant Science Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            LIU, JUNQI - UNIVERSITY OF MINNESOTAitem
                                            Miller, Susanitem
                                            Bucciarelli, Brunaitem
                                            FEDOROVA, MARIA - UNIVERSITY OF MINNESOTAitem
                                            MATSUMOTO, PETER - UNIVERSITY OF MINNESOTAitem
                                            GRAHAM, MICHELLE - UNIVERSITY OF MINNESOTAitem
                                            SHERRIER, JANINE - UNIVERSITY OF DELAWAREitem
                                            Samac, Deborah

                                                         - *Debby*item
                                            GANTT, STEVE - UNIVERSITY OF MINNESOTAitem
                                            Vance, Carroll
  Submitted to: American Society of Plant Biologists Annual Meeting
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 7/25/2004
                                    Publication  Date: 7/25/2004
                                    Citation: Liu, J., Miller, S.S., Bucciarelli, B., Fedorova, M., Matsumoto, P., Graham, M., Sherrier, J., Samac, D.A., Gantt, S., Vance, C.P. 2004. Identification and characterization of novel calcium binding proteins in the symbiosome space of M. truncatula [abstract]. American Society of Plant Biologists Annual Meeting, July 24-28, 2004, Orlando, FL. Abstract No. 24004.Interpretive Summary:
                                Technical Abstract: Calcium is a key messenger and has been implicated in the mediation of a variety of signaling processes including the symbiotic legume-rhizobium interaction. Calmodulins (CaMs) are the prevalent calcium sensor and Ca 2+/CaM complex can regulate the function of diverse proteins, such as transcription factors, metabolic enzymes, and ion channels. The specificity and subcellular nature of calcium signaling remains largely unclear. Analysis of the transcriptome in Medicago truncatula root nodules revealed a group of nodule specific transcripts encoding calmodulin-like proteins, designated as NodCaMs (for nodule specific calmodulin-like proteins). Unlike typical calmodulins (CaM) with four EF hands, these NodCaMs only have two to three EF hands. All NodCaMs are predicted to possess an N-terminal signal peptide. We have proven that fusion NodCaMs bind calcium in vitro. We also found that two of the NodCaM genes are located 2.5 kb from each other in tandem orientation. A NodCaM promoter was fused to GUS and EGFP coding region and used in the transformation of Medicago sativa. Cellular expression pattern of NodCaMs correlates with the process of nodule development. Both in situ hybridization and GUS staining showed that NodCaM expression was strong in infected cells whereas little to no expression was observed in uninfected cells in mature nodules. In addition, antibody raised against NodCaM fusion protein recognized the native NodCaMs in the symbiosome space. The subcellular localization was further confirmed by the MS/MS analysis of symbiosome space protein fraction. Taken together the genomics, biochemical, histological, and molecular data, these NodCaMs seem to represent a novel class of calcium sensors located in the symbiosome space with functions specific to root nodules.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov