U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Livestock Bio-Systems: Clay Center, NE
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    USMARC Home
                    USMARC Research Units
                        Genetics and Animal Breeding
                                    Meat Safety & Meat Quality
                                    Nutrition, Growth and Physiology
                                    Animal Health GenomicsSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Plains Area Clay Center, Nebraska U.S. Meat Animal Research Center Livestock Bio-Systems Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications
- IACUC Summaries

Title:   Author  item
                                            Kim, Jongitem
                                            Nonneman, Danny

                                                         - *Dan*item
                                            Vallet, Jeffitem
                                            Rohrer, Garyitem
                                            Christenson, Ronald
  Submitted to: Animal Genetics
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 1/5/2005
                                    Publication  Date: 4/1/2005
                                    Citation: Kim, J.G., Nonneman, D., Vallet, J.L., Rohrer, G.A., Christenson, R.K. 2005. (Brief Note) Linkage mapping of the porcine chromogranin B (CHGB) gene to chromosome 17. Animal Genetics. 36(2):168.Interpretive Summary: Chromogranins are a group of proteins that mediate sorting and aggregation of hormones and neuropeptides into cellular secretory granules. One of the these proteins, Chromogranin B (CHGB; also called secretogranin I), is found in secretory granules throughout cells of the endocrine and nervous system. Because of its role in hormone secretion, CHGB is critically involved in reproductive function. Two cDNA clones, one containing the full coding region (pCHGB1; GenBank accession no. AY613916) and another containing a partial coding region (pCHGB2; GenBank accession no. AY623647) of the porcine CHGB were isolated from the Meat Animal Research Center (MARC) 2PIG cDNA library and sequenced. A single nucleotide polymorphism (SNP) in the coding region (nucleotide 1568, AY613916, and nucleotide 876, AY623647) was identified in the 2 clones. This SNP was evaluated in the eight parents (seven F1 sows and one White composite boar) of the MARC Swine Reference Population. The SNP was confirmed and its inheritance could be observed in the piglets from 5 of the 7 F1 sows and 1 boar in the MARC Swine Reference Population. Using these inheritance patterns, the CHGB gene was determined to be on swine chromosome 17. This is consistent with previous observations indicating that the human CHGB gene is located in an area of human chromosome 20 that is similar to the area of swine chromosome 17 containing the CHGB gene.
                                Technical Abstract: Chromogranins mediate sorting and aggregation of hormones and neuropeptides into cellular secretory granules. Because of its role in hormone secretion, CHGB is critically involved in reproductive function. Two cDNA clones, one containing the full coding region (pCHGB1; GenBank accession no. AY613916) and another containing a partial coding region (pCHGB2; GenBank accession no. AY623647) of the porcine CHGB, were isolated from the Meat Animal Research Center (MARC) 2PIG expressed sequence tag (EST) library. A silent single nucleotide polymorphism (SNP) in the coding region (nucleotide 1568, AY613916, and nucleotide 876, AY623647) was identified in the 2 clones. Primers were designed to amplify a 941 bp product in the coding region of the gene. The forward and reverse primers correspond to bases 1056-1075 and 1996-1975 of the porcine CHGB cDNA (GenBank accession no. AY613916). This SNP was evaluated in the eight parents (seven F1 sows and one White composite boar) of the MARC Swine Reference Population by sequencing PCR products amplified from genomic DNA. The A/G polymorphism at nucleotide 1568 of GenBank (accession no. AY613916) was heterozygous in 5 of the 7 F1 sows and 1 boar of the MARC Swine Reference Population. An assay was designed to genotype this polymorphism by primer extension with analyte detection on a MALDI-TOF mass spectrometer using a pair of internal primers. The internal forward and tailed reverse primers correspond to bases 1459-1480 and 1660-1639 of the porcine CHGB cDNA (GenBank accession no. AY613916). This marker generated 64 informative meioses in the MARC swine reference population. The CHGB gene was mapped using CRI-MAP to chromosome 17, relative position 30.2 cM, which is the same position as microsatellite marker SWR1133 on the current MARC swine chromosome 17 linkage map (http://www.marc.usda.gov/). The most significant two-point linkage of CHGB detected was with SWR1133 (LOD = 18.06) at 0.0 recombination.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov