U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 close



Research
                Research Home
                            National Programs
                            Research Projects
                            Scientific Manuscripts
                            International Engagement
                            Scientific Software/Models
                            Databases and Datasets
                            Office of Scientific Quality Review
            Media
                News & Features
                            Multimedia
                            Briefing Room
                            Down on the Farm
                            Press Room
                            Science in Your Shopping Cart
                            Scientific Discoveries
                            Tellus
                            Under the Microscope
            About ARS
                About ARS Home
                            Staff Directory
                            Labs and Research Centers (Map)
                            Headquarter Offices
                            Organizational Chart
                            Employee Services (REE Employees Only)
                            Office of Outreach, Diversity, and Equal Opportunity
            Work With Us
                Work With Us Home
                            ARS Vacancies at USAJOBS
                            Careers at ARS Information
                            Scientific Collaborations
                            Extramural Agreements
                            1890 Faculty Research Sabbatical ProgramSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            PARK, YOUNG-HOON - UNIVERSITY OF CA. - DAVISitem
                                            Ulloa, Mauricioitem
                                            SICKER, BRAD - UNIVERSITY OF CA. - DAVISitem
                                            WILKINS, THEA - UNIVERSITY OF CA. - DAVIS
  Submitted to: National Cotton Council Beltwide Cotton Conference
                                    Publication Type: Proceedings
                                    Publication Acceptance Date: 1/6/2004
                                    Publication  Date: 1/6/2004
                                    Citation: PARK, Y.H., ULLOA, M., SICKER, B.A., WILKINS, T. 2004. DEVELOPMENT AND CHARACTERIZATION OF FIBER EST-DERIVED MICROSATELLITES IN COTTON (GOSSYPIUM SPP). NATIONAL COTTON COUNCIL BELTWIDE COTTON CONFERENCE. p. 1187-1191.Interpretive Summary:
                                Technical Abstract: A new set of microsatellite or simple sequence repeat (SSR) markers were developed from the sequence information of a fiber EST database from the diploid species Gossypium arboreum.  Four hundred EST-derived SSR primer sets were designed and tested for PCR amplification. Polymorphic loci among six species of cotton were observed from 233 (73.7 %) SSR markers and 83 were monomorphic. Between G. hirsutum and G. barbadense, 103 markers were polymorphic, while the polymorphism within these two tetraploid species was low 4.7 % and 3.8 %, respectively. One hundred twenty-three SSR markers that were polymorphic among the species showed sequence similarity to the genes with known function including fiber elongation-related genes. Based on PCR amplification, the transferability of EST-derived SSR marker from the diploid species G. arboreum was high (89.9 %) across the six Gossypium species. Most of these SSR markers provide information about functional gene, demonstrated portability and strength, and may be useful for diverse genome analysis and for the improvement of the cotton crop through marker-assisted selection.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov