U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 close



Research
                Research Home
                            National Programs
                            Research Projects
                            Scientific Manuscripts
                            International Engagement
                            Scientific Software/Models
                            Databases and Datasets
                            Office of Scientific Quality Review
            Media
                News & Features
                            Multimedia
                            Briefing Room
                            Down on the Farm
                            Press Room
                            Science in Your Shopping Cart
                            Scientific Discoveries
                            Tellus
                            Under the Microscope
            About ARS
                About ARS Home
                            Staff Directory
                            Labs and Research Centers (Map)
                            Headquarter Offices
                            Organizational Chart
                            Employee Services (REE Employees Only)
                            Office of Outreach, Diversity, and Equal Opportunity
            Work With Us
                Work With Us Home
                            ARS Vacancies at USAJOBS
                            Careers at ARS Information
                            Scientific Collaborations
                            Extramural Agreements
                            1890 Faculty Research Sabbatical ProgramSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            HARRIS, LINDA - AGRI-FOOD CANADA, OTTAWAitem
                                            Alexander, Nancyitem
                                            SAPARNO, AUDREY - AGRI-FOOD CANADA, OTTAWAitem
                                            BLACKWELL, BARBARA - AGRI-FOOD CANADA, OTTAWAitem
                                            McCormick, Susanitem
                                            Desjardins, Anneitem
                                            LAURIAN, ROBERT - AGRI-FOOD CANADA, OTTAWAitem
                                            TINKER, NICK - AGRI-FOOD CANADA, OTTAWAitem
                                            OUELLET, THERESE - AGRI-FOOD CANADA, OTTAWA
  Submitted to: International Fusarium Workshop
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 12/15/2004
                                    Publication  Date: 12/15/2004
                                    Citation: Harris, L., Alexander, N.J., Saparno, A., Blackwell, B., Mcalpin, C.E., Desjardins, A.E., Robert, L., Tinker, N., Ouellet, T. 2004. Functional genomics of fusarium graminearum [abstract]. Proceedings of the 2nd International Symposium on Fusarium Head Blight. p. 567.Interpretive Summary:
                                Technical Abstract: We are conducting gene profiling experiments using Fusarium graminearum, approximately 4.5K uniEST cDNA microarrays and targeted gene disruptions to identify and functionally test candidate genes suspected to be involved in F. graminearum (Fg) pathogenicity.  Fourteen F. graminearum cDNA libraries have been constructed from fungal cultures grown under a variety of conditions to generate a collection of approximately 10,000 expressed sequence tags (ESTs) which group into approximately 5,500 contigs/singletons.  A 4.5K unigene F. graminearum cDNA microarray has been printed in-house and array hybridization experiments are underway to explore metabolite biosynthesis and genes induced upon plant contact.  Initial array hybridizations have compared expression profiles of Fg grown in liquid culture under conditions of trichothecene production over a 12-day period versus Fg under the same conditions at time 0. Metabolite analysis (HPLC/NMR) of ethyl acetate extracts of these liquid cultures has indicated the production of near equimolar amounts of 15A-DON and butenolide.  Functional analysis of candidate genes through gene disruption or modification is the focus of a collaborative project between the USDA and Agriculture & Agri-Food Canada.  For example, an analysis of Fg ESTs revealed a hotspot of gene expression from a putative biosynthetic gene cluster on Fg genome contig 1.324.  Eight consecutive predicted genes, FG08077-FG08084, are represented by a total of 51 Fg ESTs isolated from trichothecene-producing culture conditions.  In addition, five of the genes are represented by six ESTs originating from Fg-infected wheat or barley libraries, suggesting these genes are expressed in planta.  These genes include an oxidoreductase, a cytochrome P450, an oxygenase, an acetyltransferase, and a transporter.  Northern analysis conducted on seven of the eight genes showed coordinated induction of gene expression, beginning at 4 days post induction and peaking at approximately 10 days post induction in liquid culture.  Expression analysis of the eighth gene is in progress.  Four of these genes have been initially targeted for gene disruption.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov