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Title:   Author  item
                                            Berhow, Markitem
                                            Johnson, Ericitem
                                            Dowd, Patrickitem
                                            Duval, Sandra
  Submitted to: Phytochemical Society of North America Meeting and Newsletter
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 7/12/2006
                                    Publication  Date: 7/12/2006
                                    Citation: Berhow, M.A., Johnson, E.T., Dowd, P.F., Duval, S.M. 2006. Extraction and characterization of anthocyanins from pigmented flowers and black seed hulls [abstract]. Phytochemical Society of North America Meeting and Newsletter. p. 22Interpretive Summary:
                                Technical Abstract: New methodology has been developed for the preparative scale extraction and purification of anthocyanins from "black" seed coats and hulls.  A combination of physical fractionation, extraction, flash chromatography and preparative chromatography has resulted in the isolation of gram quantities of purified anthocyanins from petunia and gentium flowers, and black soybeans, black beans and cuphea seed hulls.  The anthocyanin structures were determined by LC-MS.  The methodology developed can be used to quantitate the levels of the anthocyanins in various cultivars and growing conditions.  The quantities being isolated allow for the evaluation of their insecticidal, fungicidal and herbicidal activities in a variety of bioassay systems.


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