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ARS Home Northeast Area Beltsville, Maryland (BHNRC) Beltsville Human Nutrition Research Center Food Composition and Methods Development Laboratory Research Publications at this Location





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Title:   Author  item
                                            KELM, MARK - 1235 15 00item
                                            Flanagan, Vincentitem
                                            Pawlosky, Robertitem
                                            Novotny, Janetitem
                                            Clevidence, Beverlyitem
                                            Britz, Steven
  Submitted to: Lipids
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 10/11/2001
                                    Publication  Date: 12/1/2001
                                    Citation: Kelm, M., Flanagan, V.P., Pawlosky, R.J., Novotny Dura, J., Clevidence, B.A., Britz, S.J. 2001. Determination of 13c-labeled and endogenous beta-carotene, lutein, and vitamin a in human plasma using quantitative mass spectrometry. Lipids.36:1277-1282.Interpretive Summary:   In addition to the nutritional value of betacarotene as a   pro-vitamin A compound, carotenoids and related xanthophylls   may possess other beneficial nutritional effects, such as   those related to their antioxidant properties.  In support   of potential human nutrition investigations, three    definitive methods utilizing (NCI) mass spectrometry were   advanced for the determination of endogenous (12C-compounds)   and 13C-lableled betacarotene, -lutein and -retinol (derived   from the labeled betacarotene) in plasma from a subject who   was fed a typical serving of 13C-labeled kale. The   determination of the retinol isotopomers from plasma was   carried out using labeled internal standards by GC-MS with   NCI detection.  Information coming from this study is of    importance to nutritionists and policy-makers who are in a   position to make decisions regarding the adequacy of certain   nutrients present in foods to meet Americans dietary   requirements.
                                Technical Abstract:   Quantitative procedures employing liquid-chromatography /   particle beam-mass spectrometry (LC/PB-MS) and gas   chromatography-mass spectrometry (GC-MS) were developed for   the determination of the endogenous and 13C-labeled   betacarotene, -lutein and -retinol in plasma of a subject    who consumed kale (Brassica oleracea), which had been grown   in a 13CO2 enriched atmosphere. Betacarotene and lutein were   analyzed using LC/PB-MS applying HPLC separation procedures   to resolve the analytes.  The concentrations of the   betacarotene in the plasma over a several week period were   determined using 2H8-betacarotene as an internal standard.   The total plasma concentrations of all trans-lutein was   quantified by HPLC analysis with UV detection using beta-    apo-8'-carotenal as an internal standard and the ratio of   the 13C:12C isotopomers of lutein were determined by PB-MS.   The retinol isotopomers were collected from HPLC fractions   of the plasma extract and then analyzed as the   trimethylsilyl ethers by GC-MS. The retinol isotopomers were   quantified using 2H4-retinol. These methods demonstrate the   application of highly sensitive MS procedures for the   determination of carotenoids and vitamin A.


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