U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 close



Research
                Research Home
                            National Programs
                            Research Projects
                            Scientific Manuscripts
                            International Engagement
                            Scientific Software/Models
                            Databases and Datasets
                            Office of Scientific Quality Review
            Media
                News & Features
                            Multimedia
                            Briefing Room
                            Down on the Farm
                            Press Room
                            Science in Your Shopping Cart
                            Scientific Discoveries
                            Tellus
                            Under the Microscope
            About ARS
                About ARS Home
                            Staff Directory
                            Labs and Research Centers (Map)
                            Headquarter Offices
                            Organizational Chart
                            Employee Services (REE Employees Only)
                            Office of Outreach, Diversity, and Equal Opportunity
            Work With Us
                Work With Us Home
                            ARS Vacancies at USAJOBS
                            Careers at ARS Information
                            Scientific Collaborations
                            Extramural Agreements
                            1890 Faculty Research Sabbatical ProgramSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Jenkins, Markitem
                                            Miska, Kateitem
                                            KLOPP, S - TOWNSENDS POULTRY
  Submitted to: Avian Diseases
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 10/13/2005
                                    Publication  Date: 3/1/2006
                                    Citation: Jenkins, M.C., Miska, K.B., Klopp, S. 2006. Application of polymerase chain reaction based on its1 rdna to speciate eimeria. Avian Diseases. 50:110-114.Interpretive Summary: Avian coccidiosis remains a significant disease problem for the U.S. and worldwide poultry industries.  The use of live oocyst vaccines to prevent coccidiosis is gaining greater acceptance by the poultry industry, especially with increasing drug resistance in Eimeria and greater consumer pressure to discontinue use of drugs in animal feed. Effective application of live oocyst vaccines requires some knowledge of the species compostion of Eimeria in a poultry facility so that vaccines containing these species are used for immunization.  The present study provides a rapid technique for directly assessing the species composition of Eimeria in poultry litter.  This technique is based on the use of polymerase chain reaction to amplify species specific regions of DNA.  The technique should help poultry producers and vaccine manufacturers prevent outbreaks of avian coccidiosis.
                                Technical Abstract: A method was developed to recover Eimeria spp. oocysts directly from poultry litter and determine the species composition of Eimeria present using polymerase chain reaction (PCR) based on the ITS1 rDNA sequence.  The species composition of Eimeria oocysts was also compared before and after propagation in susceptible chickens to determine if the relative proportion of each species changed after expansion.  In samples from two broiler operations, ITS1-PCR was able to detect Eimeria spp. oocysts recovered from litter samples, with E. acervulina, E. maxima, and E. praecox being the predominant species present therein.  Although E. tenella was found in one sample, the other species- E. brunetti, E. necatrix, and E. mitis were not detected.  The species composition as determined by ITS1-PCR did not appear to appreciably alter after expansion in susceptible chickens.   The described method represents a rapid means for determining the major Eimeria species in a poultry operation, and may be helpful in choosing a particular live oocyst vaccine formulation to protect chickens against coccidiosis.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov