U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 close



Research
                Research Home
                            National Programs
                            Research Projects
                            Scientific Manuscripts
                            International Engagement
                            Scientific Software/Models
                            Databases and Datasets
                            Office of Scientific Quality Review
            Media
                News & Features
                            Multimedia
                            Briefing Room
                            Down on the Farm
                            Press Room
                            Science in Your Shopping Cart
                            Scientific Discoveries
                            Tellus
                            Under the Microscope
            About ARS
                About ARS Home
                            Staff Directory
                            Labs and Research Centers (Map)
                            Headquarter Offices
                            Organizational Chart
                            Employee Services (REE Employees Only)
                            Office of Outreach, Diversity, and Equal Opportunity
            Work With Us
                Work With Us Home
                            ARS Vacancies at USAJOBS
                            Careers at ARS Information
                            Scientific Collaborations
                            Extramural Agreements
                            1890 Faculty Research Sabbatical ProgramSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            HUFF, K - PURDUE UNIVERSITYitem
                                            BANADA, P - PURDUE UNIVERSITYitem
                                            BAE, E - PURDUE UNIVERSITYitem
                                            BAYRAKTAR, B - PURDUE UNIVERSITYitem
                                            RAJWA, B - PURDUE UNIVERSITYitem
                                            ROBINSON, J - PURDUE UNIVERSITYitem
                                            HIRLEMAN, E - PURDUE UNIVERSITYitem
                                            Richards, Garyitem
                                            BHUNIA, A - PURDUE UNIVERSITY
  Submitted to: American Society for Microbiology
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 1/31/2006
                                    Publication  Date: 5/21/2006
                                    Citation: Huff, K.R., Banada, P.P., Bae, E., Bayraktar, B., Rajwa, B., Robinson, J.P., Hirleman, E.D., Richards, G.P., Bhunia, A.K. 2006. Detection and identification of foodborne pathogens to genus and species levels using a noninvasive modified light scatterometer - bardot. American Society for Microbiology. P-075.Interpretive Summary:
                                Technical Abstract: Specific and rapid detection of pathogens in clinical, food, water or air samples, has proven to be a very difficult task but is essential for developing counter measures against food safety and biosecurity. Optical light scattering has been widely used for characterizing microorganisms in suspensions, but success has been limited. In this study, an improved light scatterometer - Bacteria Rapid Detection using Optical Scattering Technology (BARDOT), as a non-invasive optical light sensor has been developed for bacterial colony identification on a nutrient agar plate.  Bacterial colonies of Listeria, Salmonella, Vibrio, Enterobacter, Enterococcus, Escherichia, and Bacillus were tested using BARDOT system. Purity of each bacterial culture was tested and identified using Gram staining, CAMP test (for Listeria), PCR and ribotyping. The cultures were grown in BHI broth, serially diluted and spread plated to obtain ~20-30 colonies/plate. The colonies were allowed to grow to ~2 mm (12-36 h at 37 C). Images of one hundred colonies from each culture were captured using the BARDOT system and were analyzed by extracting features using Zernike moments and grouped using principle component analysis (PCA) and K-means clustering.  All the cultures mentioned above gave unique light scattering patterns. Each image had a characteristic circular ring pattern or spike like structures or a fuzzy uneven appearance differing for each bacterial species. The image patterns for each genus and species within the genus were very distinct and even could be easily identified by untrained human eyes.  BARDOT has proven to be an elegant powerful tool in rapid detection and identification of bacterial colonies grown on solid agar surfaces in 5 min.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov