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Title:   Author  item
                                            SELLERS, HOLLY - UNIV OF GA - ATHENS, GAitem
                                            LINNEMANN, ERICH - UNIV OF GA - ATHENS, GAitem
                                            Kapczynski, Darrell
  Submitted to: American Association of Avian Pathologists
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 2/8/2005
                                    Publication  Date: 7/16/2005
                                    Citation: Sellers, H.S., Linnemann, E.G., Kapczynski, D.R. 2005. Use of a multiplex RT-PCR to differentiate lentogenic NDV from END [abstract]. American Association of Avian Pathologists. p. 32.Interpretive Summary:
                                Technical Abstract: A multiplex RT-PCR has been designed to differentiate lentogenic Newcastle disease viruses from exotic Newcastle disease (END).  Using computer sequence analysis and published sequences (GenBank), we have constructed lentogenic (B1/LaSota) and END (CA02)-specific primer sets.  The primers amplify regions within the phosphoprotein and fusion protein genes of NDV.   The primer sets have been tested in uniplex RT-PCR assays and amplify the predicted target templates.  Our preliminary evaluation of the primers in a multiplex RT-PCR format suggests the primers will be effective in discriminating lentogenic and velogenic NDVs.  The multiplex RT-PCR parameters have been optimized. RNA from archived NDV field isolates and END experimentally infected and uninfected chickens will be tested.  The sensitivity and specificity is currently being determined


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