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Title:   Author  item
                                            Anderson, Robinitem
                                            Callaway, Todditem
                                            Van Kessel, Jo Annitem
                                            Nisbet, David
  Submitted to: Western Section of Animal Science Proceedings
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 6/21/2001
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: Ruminal methane (CH4) production is an inefficient process that results in losses of 2-12% of gross energy intake. Presently, we tested the effect of three potential inhibitors of ruminal CH4 production in vitro. Ruminal fluid was incubated (39 deg C) for 24 h under 100% CO2 in closed tubes (10 ml/tube) supplemented with 72 mM formate and 12 mM 2-nitropropanol (2NPOH), ,nitroethane (NE) or nitroethanol (NeOH). Control cultures contained no added nitrocompound. Presented values are the mean +/- SE from n = 3 and tests for significance were accomplished using an analysis of variance. Methane production was reduced (P<0.05) from control incubations (27.6 +/- 2.1 umol/ml rumen fluid) by more than 10-fold by all nitrocompounds (2.6 +/- 0.4, 1.5 +/- 0.4 and 1.4 +/- 0.1 umol/ml for 2NPOH, NeOH and NE, respectively). Concentrations of acetate, propionate and butyrate produced by nitrocompound supplemented cultures were not different (P>0.05) from  those produced by control cultures (62.1 +/- 5.1, 15.8 +/- 1.1 and 11.0 +/ 1.91 umol/ml, respectively) indicating that reductant was not redirected toward the production of more reduced fermentation acids. However, H2 did accumulate in incubations supplemented with the nitrocompounds (2.8 +/- 0.2, 2.1 +/- 0.6 and 1.9 +/- 0.2 umol/ml for 2NPOH, NeOH and NE, respectively). Thus, energetic efficiencies associated with microbial interspecies H2 transfer were diminished which suggests that addition of other H2 oxidizing bacteria to the nitrocompound supplemented cultures may preserve an electron sink typically beneficial to the fermentation. Future research in this area may lead to the development of strategies that reduce ruminal CH4 production.


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