U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 close



Research
                Research Home
                            National Programs
                            Research Projects
                            Scientific Manuscripts
                            International Engagement
                            Scientific Software/Models
                            Databases and Datasets
                            Office of Scientific Quality Review
            Media
                News & Features
                            Multimedia
                            Briefing Room
                            Down on the Farm
                            Press Room
                            Science in Your Shopping Cart
                            Scientific Discoveries
                            Tellus
                            Under the Microscope
            About ARS
                About ARS Home
                            Staff Directory
                            Labs and Research Centers (Map)
                            Headquarter Offices
                            Organizational Chart
                            Employee Services (REE Employees Only)
                            Office of Outreach, Diversity, and Equal Opportunity
            Work With Us
                Work With Us Home
                            ARS Vacancies at USAJOBS
                            Careers at ARS Information
                            Scientific Collaborations
                            Extramural Agreements
                            1890 Faculty Research Sabbatical ProgramSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Emche, Sarahitem
                                            Bailey, Bryan
  Submitted to: Mid Atlantic Plant and Molecular Biology Proceedings
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 7/10/2001
                                    Publication  Date: 7/14/2001
                                    Citation: Keates, S.E., Bailey, B.A. 2001. Differential gene expression in weed species treated with a necrosis-inducing peptide [abstract]. Mid Atlantic Plant and Molecular Biology Proceedings.Interpretive Summary:
                                Technical Abstract: Determining how fungal peptides induce plant responses may further our understanding of the disease resistance mechanisms protecting agricultural crops, and the factors influencing the susceptibility of weed species to potential bioherbicidal agents occurring naturally in the environment. A 24-kDa peptide isolated from culture filtrates of Fusarium oxysporum, Nep1, ,elicits physiological responses in a broad range of plant species. Nep1 induces necrosis in a variety of weed species within 48 hours of foliar spray treatment. Dandelion (Taraxacum officionale Weber) and spotted knapweed (Centaurea maculosa Lam.) show extensive necrosis following treatment, and are being used to assess weed responses to the peptide. We are isolating RNA from treated and control plants and using differential display (DDRT-PCR) to identify genes that are differentially expressed in response to Nep1 treatment. Initial screening will focus on plant genes tha aare up regulated or down regulated within four hours of treatment with the necrosis-inducing peptide. More than 200 bands of interest have been isolated from differential display gels and will be confirmed based on replicate display profiles and Northern blots prior to cloning and sequencing. Parallel studies with Arabidopsis thaliana L., which also develops extensive necrosis following Nep1 application, may facilitate tentative identification of genes of interest based on sequence homology.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov