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Title:   Author  item
                                            CANADA, N - ICBAS, U PORTO PORTUGALitem
                                            MEIRELES, C - CECA U PORTO PORTUGALitem
                                            MEZO, M - LA CORUNA SPAINitem
                                            WARLETA, M - LA CORUNA SPAINitem
                                            CORREIA DA COSTA, J - CPIB PORTO PORTUGALitem
                                            SREEKUMAR, C - ANRI APDL BELTSVILLE MDitem
                                            Hill, Doloresitem
                                            Miska, Kateitem
                                            Dubey, Jitender
  Submitted to: Journal of Parasitology
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 2/26/2004
                                    Publication  Date: 3/24/2004
                                    Citation: Canada, N., Meireles, C.S., Mezo, M., Warleta, M., Correia Da Costa, J.M., Sreekumar, C., Hill, D.E., Miska, K.B., Dubey, J.P. 2004. First isolation of neospora caninum from an aborted bovine fetus in spain. Journal of Parasitology 90:863-864.Interpretive Summary: Neospora caninium is a single celled parasite that causes abortion in cattle worldwide.  The dog is he main reservoir host.  Scientists at the Beltsville Agricultural Resarch Center and the University of Porto Portugal have isolated N. caninum from Spain on aborted bovine fetus for the first time.  These results will be of interest to biologists, parasitologists and veterinarians.
                                Technical Abstract: Neospora caninum was isolated from the brain of a 6-mo- old aborted bovine fetus from Galicia, Spain. The fetal brain homogenate was inoculated intraperitoneally into cortisonized mice. The peritoneal exudate from the infected mice, along with mouse sarcoma cells (Tg180) was inoculated into a second group of mice and parasites were harvested from the peritoneal exudate. The parasites were  adapted to in vitro growth in Vero monolayers. The tachyzoites from the peritoneal exudate reacted positively with anti-N. caninum antibodies and not with anti- Toxoplasma gondii antibodies on indirect fluorescent antibody test. The tachyzoites were lethal to interferon gamma  gene knock out (KO) mice and could be identified  immunohistochemically in the tissues.  The identity of the parasite was also confirmed by PCR amplification of  N. caninum specific fragments.  The sequences of the amplified gene 5 fragment (GenBank accession No. AY494944) were found to be identical to that of an Austrian isolate of N. caninum, but not with that of NC-1.This is the first isolation of  viable N. caninum  from Spain.


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