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ARS Home Northeast Area Beltsville, Maryland (BHNRC) Beltsville Human Nutrition Research Center Food Composition and Methods Development Laboratory Research Publications at this Location





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Title:   Author  item
                                            Kelm, Markitem
                                            Flanagan, Vincentitem
                                            Pawlosky, Robertitem
                                            Novotny, Janetitem
                                            Britz, Steven
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 5/1/2000
                                    Publication  Date: 5/1/2000
                                    Citation: Kelm, M.A., Flanagan, V.P., Pawlosky, R.J., Novotny Dura, J., Britz, S.J. 2000. An lc/pb method for the analysis of 13 c labeled lutein and b-carotene in  kale and human plasma. (BARC Poster Day, 2000). Meeting Abstract.Interpretive Summary:
                                Technical Abstract: To determine the bioavailability of lutein and b-carotene from kale Brassica oleracea L., a LC-MS method was employed.  Extracts of kale grown under an enriched atmosphere of 13CO2 and plasma extracts from human subjects that ingested the 13C-labeled kale were subjected to LC-MS.  HPLC separations of plasma and kale  extracts were carried out under reverse phase conditions using a C18 column.  Gradient elution was used for simultaneous separation of xanthophylls and carotenoids, namely lutein and b- carotene.  The HPLC effluent was nebulized using helium and the solvent was removed under vacuum within the dual stage particle- beam interface.  In the negative chemicals ionization mode, methane was used as the reagent gas to afford xanthophyll and carotenoid ions that were detected using SIM.  Quantification was  based on the isotopic abundance ratios of uniformly labeled b- carotene (m/z 576) to the internal standard 2H8-b-carotene (m/z 544) and the ratio of uniformly labeled lutein (m/z 608) to the internal standard, b-apo-8'-carotenal (m/z 416).  This study constitutes a major initiative investigating bioavailability of nutrients from foods using the unique resources of several collaborating facilities within BARC.


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