U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Infectious Bacterial Diseases Research: Ames, IA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area Ames, Iowa National Animal Disease Center Infectious Bacterial Diseases Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            LYASHCHENKO, KONSTANTIN - CHEMBIOitem
                                            GREENWALD, RENA - CHEMBIOitem
                                            ESFANDIARI, JAVAN - CHEMBIOitem
                                            Waters, Wadeitem
                                            Palmer, Mitchellitem
                                            Whipple, Dianaitem
                                            OLSEN, JOHN - BUSCH GARDENS, FLitem
                                            BALL, RAY - BUSCH GARDENS, FLitem
                                            POLLOCK, JOHN - BELFAST, UKitem
                                            ANDERSEN, PETER - COPENHAGEN, DENMARK
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 9/7/2004
                                    Publication  Date: 9/7/2004
                                    Citation: Lyashchenko, K., Greenwald, R., Esfandiari, J., Waters, W.R., Palmer, M.V., Whipple, D.L., Olsen, J., Ball, R., Pollock, J., Andersen, P. 2004. Rapid test for Serological Detection of Tuberculosis in Multiple Animal Species [abstract]. 6th European Conference of the WDA, Uppsala, Sweden 9/8-12/04. p. 25.Interpretive Summary:
                                Technical Abstract: Tuberculosis remains a serious re-emerging disease in wildlife and zoo animals, as various species are susceptible to Mycobacterium bovis and/or M. tuberculosis. To improve tuberculosis control, new diagnostic tools that would be rapid, accurate, and host species-independent are needed. We propose novel serological methods, MAPIA (MultiAntigen Print ImmunoAssay) and the lateral-flow technology, for specific antibody detection in tuberculosis. The humoral immune responses against multiple proteins during either experimental or natural infection were characterized by MAPIA in several animal species including white-tailed deer, elephant, and gazelle. The results demonstrated the remarkable heterogeneity of antigen recognition during disease in various species. Positive associations between antibody responses and the degree of pathological change due to tuberculosis infection were detected. Serological markers of diagnostic importance were identified for each host. Elephant antibody kinetics data suggested that the MAPIA could be used for monitoring treatment in these animals. Using selected antigens, a lateral-flow test was developed for rapid antibody detection in multiple species. The test can use serum, plasma, or whole blood and provides results within 15 minutes. Advantages of the proposed serological approach for tuberculosis detection in multiple animal species will be presented.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov