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Title:   Author  item
                                            KUSTER, HELGE - BIELEFELD, GERMANYitem
                                            BECKER, ANKE - BIELEFELD, GERMANYitem
                                            Samac, Deborah

                                                         - *Debby*item
                                            TESFAYE, MESFIN - UNIVERSITY OF MINNESOTA
  Submitted to: Book Chapter
                                    Publication Type: Book / Chapter
                                    Publication Acceptance Date: 7/1/2006
                                    Publication  Date: 12/8/2006
                                    Citation: Kuster, H., Becker, A., Samac, D.A., Tesfaye, M. 2006. Transcriptomics. In: The Medicago truncatula Handbook. The Samuel Roberts Noble Foundation, Ardmore, OK. Available: http://www.noble.org/MedicagoHandbook/.Interpretive Summary:
                                Technical Abstract: This chapter presents the current status of microarray and GeneChip tools in the model legume Medicago truncatula. After a short introduction into the major tools available, detailed protocols that can be used to obtain expression profiles from cDNA macroarrays, 70mer long oligonucleotide microarrays and Affymetrix Medicago GeneChips are listed. These protocols are successfully applied in the author's laboratories to obtain expression profiles from a range of Medicago truncatula tissues and growth conditions. Depending on the equipment of expression profiling facilities, modifications might be required to suit individual demands. Therefore, please use these protocols as a starting point and adjust them after consulting experienced personnel at your local facilities.


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