U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Corn Insects and Crop Genetics Research: Ames, IA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Database Projects
                        MaizeGDB
                                    SoyBase
                                    PeanutBase
                                    Legume Information System
                                    Maize Newsletter
                                    Maize Mutant Phenotypes
                                    Maize Genetics Conference
                                    Shiny Soybase CNV
                                    Shiny Soybase SNP
                                    Common Bean and Alfalfa - PvGEA
                                    Common Bean and Alfalfa - AGEDSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area Ames, Iowa Corn Insects and Crop Genetics Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Gobelman Werner, Karinitem
                                            Wise, Roger
  Submitted to: Journal of Agricultural Genomics
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 7/1/2000
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary: Obligate fungal pathogens (e.g., rusts and mildews) are perhaps the greatest deterrent to cereal production worldwide.  Powdery mildew of grasses (barley, wheat, rye, and oats) is caused by the obligate fungal pathogen, Blumeria graminis.  Powdery mildew is consistently an important disease of barley, resulting in reduced grain yield, kernel weight, and  grain protein.  The primary means of disease control is by incorporation o genetic resistance into elite varieties.  Genes that confer resistance to disease are usually identified only by their phenotype.  Thus, in large-genome cereal crops such as barley, a map-based approach is one of the only alternatives for identifying genes that confer disease resistance. In this report, we describe the identification and characterization of RAPD (Random Amplified Polymorphic DNA)-derived markers flanking the Mla cluster for use in map-based gene isolation.  These molecular markers were critical lfor the isolation of the Mla resistance gene, the first gene cloned that confers active disease defense in small grain cereal crops.  The results described in this manuscript will impact scientists who work in the area of molecular plant pathology and molecular breeding.
                                Technical Abstract: Over 30 variants of the Mla (powdery mildew) resistance cluster on chromosome 5 (1H) have been identified in barley.  In this report, we describe the use of RAPDs (Random Amplified Polymorphic DNAs) and bulked segregant analysis to identify DNA markers flanking the Mla cluster.  Seven hundred and thirty-nine RAPD primers were used to compare pools of  recombinant lines that contained the (Mla6 + Mla14) or the (Mla13 + Ml-Ru3 resistance specificities, respectively.  A 1500-bp DNA fragment amplified by primer OPA-10 mapped between Hor1 and XChs3.  Similarly, a 950-bp DNA fragment amplified by primer UBC-465 is located between XChs3 and Xmwg068. Finally, a 1626-bp DNA fragment amplified by primer UBC-165 mapped 0.28 cM proximal to the Mla cluster.  Twenty sequenced-tagged site (STS) primers were developed from the UBC-16521626 sequence.  Eight STS products amplified from UBC-16521626 primers mapped 0.28 cM distal to the Mla  resistance cluster.  One of these STS primer pairs, designated P0 and P1034RC, was used to identify tightly linked YAC clones from the cultivars Ingrid and Franka.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov