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Title:   Author  item
                                            Thelen, Tonyaitem
                                            Taylor, Joshua

                                                         - *Bret*item
                                            LOEST, CLINT - NEW MEXICO STATE UNIVitem
                                            Wang, Shiquanitem
                                            Lewis, Gregory
  Submitted to: Journal of Animal Science Supplement
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 2/10/2005
                                    Publication  Date: 3/1/2005
                                    Citation: Thelen, T.M., Taylor, J.B., Loest, C.A., Wang, S., Lewis, G.S. 2005. Splanchnic metabolism of nutrients in response to methionine supplementation in ewes [abstract]. Journal of Animal Science. 83:(Suppl. 1)Paper No.396.Interpretive Summary: Supplemental rumen-protected methionine increased hepatic plasma flow and decreased ammonia flux across the portal-drained viscera.
                                Technical Abstract: Nulliparous yearling Rambouillet cross ewes (61.9 ± 2.6 kg BW) fitted with chronic indwelling hepatic (H), portal (P), and mesenteric vein and mesenteric artery (A) catheters were used in a randomized design to determine whether dietary methionine affected splanchnic metabolism of nutrients. Treatments were no added methionine (CON; n = 5) or 1.2 g of rumen-protected methionine (MET; Mepron M85, Degussa; n = 6) bolused twice daily at feeding (1.3 kg/d sugar beet pulp pellets, DM basis).  Arterial, P, and H blood samples were simultaneously collected before treatment (h 0) and then every 2 h for 12 h.  Continuous para-aminohippurate (3% wt/vol; 0.57 mL/min; mesenteric vein) infusion was used to estimate vessel plasma flows.  The treatment × time interaction was significant (P = 0.04) for P-A lactate concentration differences.  The lactate P-A differences were greater (P = 0.04) for MET at h 12 compared to CON (h 12 = 0.06 vs. -0.07 mM, respectively, SE = 0.04).    Methionine P-A and H-P ammonia concentration differences were lower (P < 0.05) than CON (P-A = 0.13 vs.0.22 mM and H-P = -0.11 vs. -0.20 mM, respectively, SE = 0.03).  Hepatic plasma flow was greater (P = 0.02) for MET than for CON (140.9 vs. 104.2 L/h, respectively, SE = 7.29); however, treatment did not affect portal and arterial plasma flows.  The treatment × time interaction was significant (P = 0.03) for ammonia portal-drained viscera flux, which was lower (P = 0.01) at h 4 for MET compared to CON (h 4 = 9.40 vs. 26.30 mM/h, respectively, SE = 4.60).  Methionine treatment did not affect glucose venous-arterial concentration differences or flux.  In conclusion, supplemental rumen-protected methionine increased hepatic plasma flow and decreased ammonia flux across the portal-drained viscera.


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