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Title:   Author  item
                                            RONNING, CATHERINE - INST. GENOMIC RESEARCHitem
                                            HAYMES, KENNETH - D2 BIOTECHNOLOGIES, INC.item
                                            SANFORD, LIND - RETIRED VEGETABLE LABitem
                                            Stommel, John
  Submitted to: American Journal of Potato Research
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 9/23/2003
                                    Publication  Date: 12/23/2003
                                    Citation: Ronning, C.M., Haymes, K.M., Sanford, L.L., Stommel, J.R. 2003. An AFLP marker-based linkage map of Solanum chacoense Bitter chromosome 1. Tomato Genetics Cooperative Report 53:31-35.Interpretive Summary: Solanum chacoense Bitter is a wild potato species related to the cultivated potato species S. tuberosum L.  The species is of interest to plant breeders because some individuals produce foliar-specific leptine glycoalkaloids which are believed to be factors in the resistance of S. chacoense to the Colorado  potato beetle.  Five segregating plant populations were developed from crosses of S. chacoense individuals that varied for leptine content (high vs. low leptine content).  These populations were screened with several classes of molecular markers.  Utilizing software for the calculation of genetic linkage maps, the data obtained was utilized to construct linkage maps for the populations developed.  Genetic linkage maps offer opportunities for plant breeders to develop  marker-assisted selection strategies for plant improvement and map-based cloning in molecular genetic research.  The results presented are intended for further use by programs active in marker-based research in Solanaceous species and  provide additional marker frameworks for integration into existing marker-based maps.
                                Technical Abstract: Solanum chacoense Bitter is a wild species related to S. tuberosum L., the cultivated potato.  The species is of interest to plant breeders because some individuals produce foliar-specific leptine glycoalkaloids which are believed to be factors in the resistance of S. chacoense to the Colorado  potato beetle.  Five related segregating F1 families of S. chacoense, developed from crosses between high x low and high x high leptine-producing selections, were utilized to construct molecular marker-based linkage maps using primarily AFLP, as well as a number of RAPD, SSR and DS-PCR markers.  A framework of ordered markers was established for each family. Linkage groups from two sets of families generated from reciprocal crosses of high x low leptine producing parents  were subsequently merged, resulting in two maps from high x low families and a single map from a high x high leptine cross.  The results will be of interest for marker-based research in S. chacoense and related Solanaceous species and provide additional marker frameworks for integration into existing genetic maps.


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