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Title:   Author  item
                                            Lewers, Kimberlyitem
                                            NILMALGODA, S - AGRI-FOOD MANITOBA CANADAitem
                                            WARNER, A - CLEMSON UNIV. CLEMSON, SCitem
                                            KNAP, H - CLEMSON UNIV. CLEMSON, SCitem
                                            Matthews, Benjamin

                                                         - *Ben*
  Submitted to: Genome Research
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 7/14/2001
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:   The soybean cyst nematode (SCN) is the major pest of soybean and causes   an estimated $1 billion loss in soybean in the USA annually.  The most   practical management strategy has been the use of resistant cultivars.   One soybean gene conferring partial resistance to SCN is the Rhg4   resistance allele on Linkage Group A2 of the soybean genetic map.  We    cloned a large piece of DNA near this gene from the soybean cultivar   Williams 82 which is susceptible to attack by the SCN.  We identified   landmark pieces of DNA and used those   landmarks to identify DNA from the same region of a soybean accession   which is resistant to all known races of SCN.  Our studies   indicate that the Rhg4 gene may be located in a piece of this cloned DNA   that is present in resistant genome, but that is absent in the   Williams 82 genome.  This information is useful to soybean    researchers interested in cloning SCN resistance genes, because it   provides DNA markers for the region around an important gene conferring   resistance to the SCN, which could lead to cloning of the gene.
                                Technical Abstract:   The soybean cyst nematode (SCN), Heterodera glycines Ichinohe, is the   foremost pest of soybean (Glycine max L.Merr.) and is responsible for   the loss of more than $1 billion annually. The most practical management   strategy has been the use of resistant cultivars.  The rhg1 resistance   allele on Linkage Group G and the Rhg4 resistance allele on Linkage    Group A2 of the soybean genetic map are especially important. Markers   closely linked to the Rhg4 locus were used to screen a bacterial   artificial chromosome (BAC) library of susceptible 'Williams 82' and   identified a 150kb BAC. End-sequenced Eco R I subclones located to an   Apa I restriction map provided landmarks for use in identifying the same   region in a BAC library of the soybean accession PI 437.654, resistant   to all known races of SCN. Eighty-seven PI 437.654 BACs were assigned to   contigs at stringency levels ranging from 1e-05 to 1e-12. Two contigs    represented the PI 437.654 counterpart of the Williams 82 BAC. Primers   derived from end-sequences of BACs connected the two contigs. The two PI   437.654 contigs are of greater physical distance than the 150 kb   Williams 82 BAC, and some primers amplify bands from the mid-portion of   the PI 437.654 BAC contig that are not amplified from the Williams 82   BAC. These observations suggest that the Rhg4 gene may be located in an   insertion in the PI 437.654 genome relative to the Williams 82 genome.   This information is important to researchers interested in cloning   soybean cyst nematode resistance genes, because it provides molecular   markers for the region around an important gene conferring resistance to   the soybean cyst nematode, which could lead to cloning of the gene.


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