U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Corn Insects and Crop Genetics Research: Ames, IA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Database Projects
                        MaizeGDB
                                    SoyBase
                                    PeanutBase
                                    Legume Information System
                                    Maize Newsletter
                                    Maize Mutant Phenotypes
                                    Maize Genetics Conference
                                    Shiny Soybase CNV
                                    Shiny Soybase SNP
                                    Common Bean and Alfalfa - PvGEA
                                    Common Bean and Alfalfa - AGEDSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area Ames, Iowa Corn Insects and Crop Genetics Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Halterman, Dennisitem
                                            Wise, Roger
  Submitted to: Plant and Animal Genome Conference Proceedings
                                    Publication Type: Proceedings
                                    Publication Acceptance Date: 1/10/2004
                                    Publication  Date: 1/10/2004
                                    Citation: Halterman, D.A., Wise, R.P. 2004. A single amino acid substitution in the sixth leucine-rich repeat of barley Mla6 and Mla13 alleviates dependence on Rar1 for disease resistance signaling. Plant and Animal Genome Conference Proceedings. XII Conference. p. 858.Interpretive Summary:
                                Technical Abstract: Interactions between barley and the powdery mildew pathogen, Blumeria graminis f. sp. hordei, are determined by unique combinations of host resistance genes, designated Ml, and cognate pathogen avirulence genes.  These interactions occur both dependent and independent of the downstream signaling genes Rar1 and Sgt1, which are differentially required for diverse plant disease-resistance pathways.  We have isolated two new functional Mla alleles, Rar1-independent Mla7 and Rar1-dependent Mla10, as well as the Mla paralogs, Mla6-2 and Mla13-2. Utilizing the inherent diversity amongst Mla encoded proteins, we identified the only two amino acids exclusively conserved in RAR1-dependent Mla6, Mla10, Mla12, and Mla13 that differ at the corresponding position in RAR1-independent MLA1 and MLA7.  Two- and three-dimensional modeling places these residues on a predicted surface of the 6th LRR domain at positions distinct from those within the beta-sheets hypothesized to determine resistance specificity.  Site-directed mutagenesis of these residues indicates that RAR1 independence requires the presence of an aspartate at position 721, since mutation of this residue to a structurally similar, but uncharged, asparagine did not alter RAR1 signaling.  These results demonstrate that a single amino acid substitution in the 6th MLA LRR can alter host signaling but not resistance-specificity to Blumeria graminis.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov