U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Plant Science Research: St. Paul, MN
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area St. Paul, Minnesota Plant Science Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            ENDRE, G - UNIVERSITY OF MINNESOTAitem
                                            PENUELA, S - UNIVERSITY OF MINNESOTAitem
                                            FEDOROVA, M - UNIVERSITY OF MINNESOTAitem
                                            LIU, J - NOBLE FOUNDATIONitem
                                            TOWN, C - INST FOR GENOMIC RESEARCHitem
                                            GANTT, S - UNIVERSITY OF MINNESOTAitem
                                            HAHN, M - COMPLEX CARBOHYDR RES CTRitem
                                            HARRISON, M - NOBLE FOUNDATIONitem
                                            Samac, Deborah

                                                         - *Debby*item
                                            Vance, Carroll
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 7/7/2001
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: Medicago truncatula has become a useful model plant for molecular studies of nitrogen-fixing symbiotic interactions. This species is also suitable to investigate plant responses to different pathogens. Our aim is to characterize legume genes involved in responses to microbes. As of April 2001, >50,000 5' sequences have been obtained from cDNA libraries spanning  various stages of vegetative development. To identify genes induced in roots, ESTs were obtained from libraries generated after inoculation with the nitrogen-fixing bacterium Sinorhizobium, the mycorrhizal fungus Glomus, or the root rot-inducing oomycete Phytophthora or its glucan elicitor. All ESTs are in GenBank and have been used (together with other public data) to construct a gene index (MtGI) that represents a minimally redundant set of sequences. MtGI groups ESTs into tentative gene clusters (TCs) according to sequence overlap (http://www.tigr.org//tdb/mtgi). MtGI also allows  assignment of tentative function to expressed genes, based on BLAST results, and indicates expression patterns of highly expressed genes, based on tissue of origin of the ESTs. Analysis reveals many examples of tissue- specific patterns of expression. We will establish a unigene set of cDNA clones to investigate the genome-wide patterns of gene expression using hybridization of probes to DNA microarrays. As a first step, we have assembled a small scale array of ~1,000 cDNA clones ('kiloclone set'), that contains positive and negative controls and clones of known tissue-specific patterns, as well as clones with putatively interesting function. Hybridization experiments to date have assessed differences in gene expression during the early steps of the symbiotic process or during pathogenic interactions, compared to uninoculated plants.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov