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ARS Home Plains Area Fargo, North Dakota Edward T. Schafer Agricultural Research Center Cereal Crops Research Research Publications at this Location





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Title:   Author  item
                                            MANOHARAN, METHUSAMY - PLNT SCI, NDSU, FARGO, NDitem
                                            Dahleen, Lynnitem
                                            HOHN, T. - SYNGENTA, TRI. PARK, NCitem
                                            McCormick, Susanitem
                                            Alexander, Nancyitem
                                            SHWARZ, PAUL - CER SCI, NDSU, FARGO, NDitem
                                            HORSLEY, RICH - PLNT SCI, NDSU, FARGO, ND
  Submitted to: National Fusarium Head Blight Forum
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 11/30/2001
                                    Publication  Date: 12/1/2001
                                    Citation: Manoharan, M., Dahleen, L.S., Hohn, T., Mc Cormick, S.P., Alexander, N.J., Shwarz, P., Horsley, R.D. 2001. Transformation of a commercial barley cultivar with genes for resistance to fusarium head blight. National Fusarium Head Blight Forum Proceedings, p. 21.Interpretive Summary:
                                Technical Abstract: Fusarium head blight, incited primarily by Fusarium graminearum, has caused devastating losses to barley since the 1990's Production of the mycotoxin deoxynivalenol (DON) by F. graminearum is harmful to humans and livestock. Expressing certain anti-toxin genes such as TR/101 and PDR5 could improve resistance to fungal infection and reduce DON levels. TR/101  encodes a 3-OH trichothecene acetyltransferase that converts DON to a less toxic acetylated form. PDR5, an ATP-binding cassette, acts as an efflux transporter, shunting DON across the plasma membrane from the interior of the cell. We have transformed the commercial malting barley cultivar Conlon with these genes to reduce DON levels in infected grain. Ten-day old calli derived from immature embryos were co-bombarded with the herbicide-resistance gene bar as the selectable marker. Putative transgenic plants were confirmed by Southern analysis. A total of seven  independent events with TR/101 and six with PDR5 were recovered. Northern analysis indicated the expression of PDR5. Expression of TR/101 was confirmed by detecting acetyltransferase activity in seeds of the transgenic plants. T2 lines of three events with TR/101 and two events with PDR5 were field tested for disease and toxin level. Both genes appeared to reduce FHB infection and PDR5 also may reduce DON accumulation.


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