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Title:   Author  item
                                            Waters, Wadeitem
                                            Palmer, Mitchellitem
                                            Pesch, Bruceitem
                                            Olsen, Stevenitem
                                            WANNEMUEHLER, M - IOWA STATE UNIV., AMESitem
                                            Whipple, Diana
  Submitted to: Veterinary Immunology and Immunopathology
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 10/10/2000
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary: Despite highly successful eradication efforts in several countries, tuberculosis of cattle remains a serious health concern worldwide.  While diagnostic reagents are available, improved techniques are needed for earlier detection of infected cattle.  A better understanding of host responses to infection, however, is needed to develop improved methods of  diagnosis.  In this study, specific cell types responding to the bacterium within infected cattle were determined as well as those cells responding to diagnostic reagents administered to infected cattle.  Knowledge obtained from this study will assist in the development of new reagents for the early detection of tuberculosis infection of cattle.
                                Technical Abstract: Despite highly successful eradication efforts in several countries, Mycobacterium bovis infection of cattle remains a significant health concern worldwide.  Immune mechanisms of resistance to and/or clearance of M. bovis infection of cattle are unclear.  Recent studies have provided evidence supporting a role for CD4+, CD8+, and gamma/delta TCR+ T cells in  the response of cattle to M. bovis.  We utilized a flow cytometric-based proliferation assay to determine the relative contribution of individual lymphocyte subsets in the response to M. bovis infection and/or sensitization with mycobacterial purified protein derivative (PPD). Peripheral blood mononuclear cells (PBMC) from M. bovis-infected cattle proliferated in response to in vitro stimulation with M. bovis PPD.  CD4+ T and gamma/delta TCR+ cells were the predominate subsets of lymphocytes responding.  Gamma/delta TCR+ cells also proliferated in nonstimulated  cultures; however, the gamma/delta TCR+ cell proliferative response of infected cattle was significantly (P less than 0.05) greater in PPD- stimulated cultures as compared to nonstimulated cultures.  Intradermal injection of PPD for comparative cervical testing (CCT) induced a boost in the in vitro proliferative response of CD4+, but not gamma/delta TCR+ cells of infected cattle.  Administration of PPD for CCT also boosted interferon-gamma production by PBMC of infected cattle following in vitro stimulation with M. bovis PPD.  Injection of PPD for CCT did not elicit a proliferative or IFN-gamma response in noninfected cattle.  These data indicate that CD4+ and gamma/delta TCR+ cells of M. bovis-infected cattle proliferate in a recall response to M. bovis PPD and that the CD4+ cell response is boosted by intradermal injection with PPD for CCT.


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