U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Meat Safety and Quality: Clay Center, NE
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    USMARC Home
                    USMARC Research Units
                        Genetics and Animal Breeding
                                    Livestock Bio Systems
                                    Nutrition, Growth and Physiology
                                    Animal Health GenomicsSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Plains Area Clay Center, Nebraska U.S. Meat Animal Research Center Meat Safety and Quality Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications
- IACUC Summaries

Title:   Author  item
                                            Gallagher, Genevieveitem
                                            Berry, Elaineitem
                                            Rivera Betancourt, Mildreditem
                                            Arthur, Terranceitem
                                            Nou, Xiangwuitem
                                            Koohmaraie, Mohammad
  Submitted to: Journal of Food Protection
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 6/4/2002
                                    Publication  Date: 10/20/2002
                                    Citation: Barkocy-Gallagher, G.A., Berry, E.D., Rivera Betancourt, M., Arthur, T.M., Nou, X., Koohmaraie, M. 2002. Development of methods for the recovery of Escherichia coli O157:H7 and Salmonella from beef carcass sponge samples and bovine fecal and hide samples. Journal of Food Protection. 65(10):1527-1534Interpretive Summary: E. coli O157:H7 and Salmonella are foodborne pathogens that can be associated with raw or undercooked beef. Therefore, it is important to measure how often these bacteria are present in cattle feces and on hides and carcasses. Methods were developed to recover both types of bacteria from the same samples, because such methods were not available. The new methods, called the MRU and MRU-TT methods, rely on steps to increase the numbers of the bacteria and specifically recover them. These methods appear to be at least as good as other methods used to recover E. coli O157:H7 and Salmonella from separate samples. Significantly, the new methods also can be used to determine how often these bacteria are present in samples at the same time.
                                Technical Abstract: Culture methods were developed for concurrent recovery of Escherichia coli O157:H7 and Salmonella from bovine carcass, fecal, and hide samples. Several enrichment conditions were tested based on overall growth of pure cultures; tryptic soy broth for 2 h at
25 deg C, then for 6 h at 42 deg C, was selected for use. Immunomagnetic separation (IMS) was incorporated for sensitivity and selectivity, with a post-IMS enrichment for recovery of Salmonella as recommended by the manufacturer. Selective agars for plating after IMS were identified based on ease of target colony identification. Sorbitol MacConkey agar supplemented with cefixime and potassium tellurite and Rainbow agar supplemented with novobiocin and potassium tellurite were chosen for recovery of E. coli O157:H7. Brilliant green agar with sulfadiazine and Hektoen enteric agar supplemented with novobiocin were selected for recovery of Salmonella. The resulting methods were evaluated along with standard or previously used methods for recovery of E. coli O157:H7 and Salmonella from bovine feces, hide samples, and carcass sponge samples. The "MRU" methods performed at least as well as the established methods, except that a secondary enrichment in tetrathionate (TT) broth prior to IMS was required for optimal recovery of Salmonella from feces. Thus, the MRU and MRU-TT methods are effective for recovery of both E. coli O157:H7 and Salmonella from the same bovine carcass, hide, or fecal samples.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov