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Title:   Author  item
                                            MIN, WONGI - CHANGWON UNIV, KOREAitem
                                            KIM, JIN-KYOO - CHANGWON UNIV, KOREAitem
                                            Lillehoj, Hyunitem
                                            SOHN, EUN - USDA:ARS:BA:ANRI:PBESLitem
                                            HAN, JAE - SEOUL UNIV, KOREAitem
                                            SONG, KI - SEOUL UNIV, KOREAitem
                                            LILLEHOJ, ERIK - UNIV MD, COLLEGE PARK
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 8/1/2001
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: The chicken monoclonal antibody, 6D-12-G10, reacts with an apical complex protein at the anterior tip of E. acervulina sporozoites that inhibits parasite invasion in vitro.  Because this mAb was produced at low amount from the original hybridoma cells, an scFv antibody was constructed by amplification of the corresponding VH  and VL genes and expressed in E. coli.  The scFv antibody was produced at a minimum of 7 mg/l and exhibited virtually identical antigen reactivity as the original mAb.


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