U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Crop Production and Pest Control Research: West Lafayette, IN
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area West Lafayette, Indiana Crop Production and Pest Control Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications
- Small Grains Viral Disease Laboratory
- Corn and Sorghum Fungal Disease Laboratory
- Host Plant Resistance to Insects Laboratory
- Wheat/Hessian fly Interactions Laboratory
- Small Grains Fungal Disease Laboratory
- Soybean Quality Improvement Laboratory
- Uniform Soybean Tests, Northern Region

Title:   Author  item
                                            Williams, Christieitem
                                            Goodwin, Stephen

                                                         - *Steve*item
                                            OHM, HERBERT - PURDUE UNIVERSITYitem
                                            Anderson, Josephitem
                                            Crane, Charlesitem
                                            Scofield, Steven

                                                         - *Steve*item
                                            LOHRET, T - CURAGEN CORPitem
                                            CRASTA, O - CURAGEN CORP
  Submitted to: Entomological Society of America Regional Meetings
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 11/15/2002
                                    Publication  Date: 3/23/2003
                                    Citation: Williams, C.E., Goodwin, S.B., Ohm, H.W., Anderson, J.M., Crane, C.F., Scofield, S.R., Lohret, T.A., Crasta, O.R. 2003. Transcription profiling of wheat defense responses:  hessian fly versus microbial pathogens. In: Proceedings of the Entomological Society of America Regional Meetings. Entomological Society of American-North Central Branch, March 23-26, 2003, Madison, Wisconsin. Available: http://esa.ent.iastate.edu/confreg/?gridaction=viewonepresentation&year=2003 &presnum=083Interpretive Summary:
                                Technical Abstract: Many genes conferring resistance to diverse pests and pathogens have been identified in wheat. However, the molecular mechanisms of plant defense are not well enough defined to determine whether resistance occurs by shared or by pathogen-specific pathways.  To identify wheat genes responding to biotic stress, plants were treated with pathogens and analyzed by GeneCalling®, an open-architecture transcript profiling technology that leads to the quantification of differentially expressed sequences (www.curagen.com).  Wheat plants were challenged with Mayetiola destructor (Hessian fly), Mycosphaerella graminicola (cause of septoria tritici leaf blotch), Fusarium graminearum (cause of head scab), and Barley Yellow Dwarf Virus.  The GeneCalling experiments profiled >12,000 cDNA fragments per sample and detected a large number that were differentially modulated by at least 1.5 times in each treatment.  For example, in the Hessian fly-infested tissue, both compatible and incompatible interactions were monitored over the first 72 hr following egg hatch, yielding 115 cDNA fragments that were differentially modulated in at least one time-point.  Sequencing and BLAST analysis of 685 fragments, taken from all treatments, revealed many genes known to be activated during defense responses in dicots, including phenylalanine ammonia lyase, glucanases, chitinases, peroxidases, and thaumatin-like proteins.  Numerous additional genes appeared to be involved in cell signaling, including kinases and phosphatases.  Approximately 10% of the sequences had no matches in the six databases searched and may represent previously unknown mechanisms of resistance.  These analyses revealed rapid resistance responses in wheat that utilize common pathways as well as unique genes that vary according to the insect or pathogen encountered.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov