U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Cereal Crops Research: Fargo, ND
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    ETSARC Research Units
                        ETSARC Home page
                                    Office of the Center Director
                                    Location Support Staff
                                    Animal Metabolism-Agricultural Chemicals Research
                                    Cereal Crops Research
                                    Insect Genetics and Biochemistry Research
                                    Sugarbeet and Potato Research
                                    Sunflower and Plant Biology ResearchSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Plains Area Fargo, North Dakota Edward T. Schafer Agricultural Research Center Cereal Crops Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Dahleen, Lynnitem
                                            McCormick, Susan
  Submitted to: North American Barley Research Workshop Proceedings
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 6/20/1999
                                    Publication  Date: N/A
                                    Citation: Dahleen, L.S., Mc Cormick, S.P. 1999. Trichothecene toxin effects on barley callus and seedling growth. North American Barley Research Workshop Proceedings, p. 56.Interpretive Summary:
                                Technical Abstract: Fusarium head blight (FHB) has caused devastating losses to barley growers since 1993.  A major component of these losses has been the formation of trichothecene toxins such as deoxynivalenol (DON) by the Fusarium. Genetic transformation of barley with genes that chemically inactivate or transport the toxins out of cells has potential for reducing DON levels.  The ability to directly select for callus cells transformed with these genes would simplify production of transgenic plants and avoid the introduction of a second selectable gene.  The objective of this study was to determine the effects of DON and the related toxin diacetoxyscirpenol (DAS) on barley callus growth and seedling germination, root and shoot growth.  Morex calli were placed on media containing 0, 1, 5, or 10 mg/L DON or DAS, and fresh weights were measured after four and eight weeks. The effects of the toxins on callus growth were small.  Although  statistically significant differences were seen at the higher toxin levels after eight weeks, the differences were not large enough for selection between toxin resistant and toxin susceptible calli. Seed were placed on the same media and toxin levels as used for callus growth, and germination rates were measured. Root and shoot length of germinated seedlings were measured for up to nine days after germination.  Neither toxin had an effect on seed germination. DON at 5 and 10 mg/L significantly reduced root growth by day 7, and 10 mg/L significantly reduced shoot growth by day 4.  The effects of DAS were greater, with all toxin levels significantly reducing root growth by day 2 and shoot growth by day 4. The results indicate that DAS may be usable for screening progeny of transgenic plants for segregation of anti-toxin genes.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov