U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Livestock Bio-Systems: Clay Center, NE
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    USMARC Home
                    USMARC Research Units
                        Genetics and Animal Breeding
                                    Meat Safety & Meat Quality
                                    Nutrition, Growth and Physiology
                                    Animal Health GenomicsSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Plains Area Clay Center, Nebraska U.S. Meat Animal Research Center Livestock Bio-Systems Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications
- IACUC Summaries

Title:   Author  item
                                            Kim, Jongitem
                                            SONG, JIAN - FORMER ARS EMPLOYEEitem
                                            Vallet, Jeffitem
                                            Rohrer, Garyitem
                                            JOHNSON, GREG - UNIV IDAHO, MOSCOWitem
                                            JOYCE, MARGARET - UNIV IDAHO, MOSCOWitem
                                            Christenson, Ronald
  Submitted to: Biology of Reproduction
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 8/28/2002
                                    Publication  Date: 3/1/2003
                                    Citation: KIM, J.G., SONG, J.H., VALLET, J.L., ROHRER, G.A., JOHNSON, G.A., JOYCE, M.M., CHRISTENSON, R.K. MOLECULAR CHARACTERIZATION AND EXPRESSION OF PORCINE BONE MORPHOGENETIC PROTEIN RECEPTOR-IB IN THE UTERUS OF CYCLIC AND PREGNANT GILTS. BIOLOGY OF REPRODUCTION. 2003. v. 68(3). p. 735-743.Interpretive Summary: Recently, it was reported that single nucleotide mutation in the bone morphogenetic protein receptor-IB (BMPR-IB) gene was associated with increased ovulation rate in the Booroola Merino breed of sheep. Interestingly, comparison of the porcine genetic map with the more detailed human genetic map suggested that the porcine BMPR-IB gene may be located in a region on porcine chromosome 8 that is associated with increased uterine capacity and litter size in swine. We cloned the full coding region for BMPR-IB gene (3559 base pair). In cyclic White composite gilts, uterine BMPR-IB gene expression was increased on Day 13 and 15, as compared to Day 10. Expression of BMPR-IB gene was localized to the inner uterine lining, including both luminal and glandular epithelial cells on Day 15. However, BMPR-IB gene expression was less responsive in pregnant gilts. The location of the BMPR-IB gene was identified on swine chromosome 8. These findings show that BMPR-IB gene expression is temporally up-regulated during the estrous cycle. The importance of this pattern of gene expression for uterine function during estrous cycle and early pregnancy remains to be determined.
                                Technical Abstract: Previous gene mapping analyses revealed a quantitative trait locus for uterine capacity on chromosome 8. Comparison of porcine and human genetic maps suggests that the bone morphogenetic protein receptor IB (BMPR-IB) gene may be located near this region. The objectives of this study were to 1) clone the full coding region for BMPR-IB, 2) examine BMPR-IB gene expression by the endometrium and its cellular localization during the estrous cycle and early pregnancy and 3) map the BMPR-IB gene. By iterative screening of an expressed sequence tag library, we obtained a 3559 bp cDNA clone including the full coding region of BMPR-IB. Endometrial BMPR-IB mRNA expression of White composite gilts was determined by Northern blotting in Day 10, 13, and 15 cyclic, and Day 10, 13, 15, 20, 30, and 40 pregnant gilts. In cyclic gilts, endometrial BMPR-IB mRNA expression was elevated on Day 13 and 15 (P < 0.01), compared to Day 10. Expression of BMPR-IB mRNA was localized in both luminal and glandular epithelium on Day 15. However, in pregnant gilts, BMPR-IB mRNA expression was not significantly different in the endometrium from Day 10 to 20, and it was significantly decreased on Day 30 and 40 (P = 0.011). The BMPR-IB gene was mapped to 108 cM on chromosome 8. These findings show that BMPR-IB mRNA expression is temporally regulated during the estrous cycle and early pregnancy in gilts; this pattern of gene expression may be important for endometrial function during the estrous cycle and early pregnancy.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov