U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 close



Research
                Research Home
                            National Programs
                            Research Projects
                            Scientific Manuscripts
                            International Engagement
                            Scientific Software/Models
                            Databases and Datasets
                            Office of Scientific Quality Review
            Media
                News & Features
                            Multimedia
                            Briefing Room
                            Down on the Farm
                            Press Room
                            Science in Your Shopping Cart
                            Scientific Discoveries
                            Tellus
                            Under the Microscope
            About ARS
                About ARS Home
                            Staff Directory
                            Labs and Research Centers (Map)
                            Headquarter Offices
                            Organizational Chart
                            Employee Services (REE Employees Only)
                            Office of Outreach, Diversity, and Equal Opportunity
            Work With Us
                Work With Us Home
                            ARS Vacancies at USAJOBS
                            Careers at ARS Information
                            Scientific Collaborations
                            Extramural Agreements
                            1890 Faculty Research Sabbatical ProgramSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Jenkins, Markitem
                                            Parker, Carolynitem
                                            TUO, WENBIN - USDA ARS APDL BELTSVILLEitem
                                            VINYARD, BRYAN - BIOMETRICS ARS USDA BELTSitem
                                            Dubey, Jitender
  Submitted to: Infection and Immunity
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 1/10/2004
                                    Publication  Date: 3/5/2004
                                    Citation: Jenkins, M.C., Parker, C.C., Tuo, W., Vinyard, B., Dubey, J.P. 2004. Inclusion of cpg adjuvant with plasmid dna coding for ncgra7 improves protection against congenital neosporosis. Infection and Immunity. 72:1817-1819.Interpretive Summary: The disease neosporosis is a major cause of abortion in dairy cattle worldwide.  Abortion occurs by congenital infection of the fetus by Neospora caninum tachyzoites during early-mid gestation.  Research has shown that immunization of cows with a whole protein extract of N. caninum tachyzoites can prevent neosporosis-associated abortion.  A desired vaccine would utilize specific antigens of the parasite that could be produced in large quantities in simple bacteria such as Escherichia coli.  The present study showed that immunization of mice with a single N. caninum antigen in the presence of an adjuvant elicited nearly complete protection against congenital infection.  While the level of protection was high with antigen alone, the addition of adjuvant increased the protective immunity by two-fold.  These results indicate that it is possible to prevent congenital neosporosis in pregnant mice by immunization with a single N. caninum antigen, but that an adjuvant may be needed to stimulate higher levels of immunity.  These findings provide a basis for vaccination trials in ruminants such as sheep and dairy cattle.
                                Technical Abstract: The present study showed that incorporating CpG adjuvant into plasmid DNA coding for NcGRA7 antigen resulted in a two-fold increase in the level of protection against congenital transfer of Neospora caninum.  The level of protection was considerably higher than that observed in pups born from dams immunized with non-recombinant plasmid.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov