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Title:   Author  item
                                            LI, GUANGXING - 1265-40-00item
                                            Lillehoj, Hyunitem
                                            MIN, WONGI - 1265-40-00
  Submitted to: Poultry Science Association Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 5/1/2002
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: We recently cloned the genes of the chicken IL-15 and IL-15 receptor (R) a proteins.  In this study, twelve monoclonal antibodies against recombinant chicken IL-15Ra were produced and characterized.  By enzyme-linked immunosorbent assay, all mAbs showed binding specificity for IL-15Ra but not IL-2 or interferon-g and identified a 25.0 kDa protein by immunoblot analysis.  Flow cytometric analysis revealed negligible expression of IL-15Ra on nonactivated lymphocytes from the spleen, thymus or bursa, low but detectable expression on macrophages and high expression on concanavalin A-activated spleen lymphoblasts.  Established chicken T cell (RP13) and macrophage (HD11) cell lines expressed substantially higher levels of IL-15Ra compared with a B cell line (RP9).  Two mAbs inhibited IL-15 dependent proliferation of T cells suggesting that the tertiary structure of the protein domain of native IL-15Ra that binds to IL-15 is preserved in the recombinant receptor molecule.  These mAbs will be useful reagents for further in vitro and in vivo studies of the biological functions of chicken IL-15 and its receptor.


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