U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Food and Feed Safety Research: College Station, TX
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Plains Area College Station, Texas Southern Plains Agricultural Research Center Food and Feed Safety Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Swaggerty, Christina

                                                         - *Christi*item
                                            FARNELL, MORGAN - TEXAS A&M UNIVitem
                                            Kogut, Michael

                                                         - *Mike*
  Submitted to: Society for Leukocyte Biology  Meetings   Proceedings
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 11/9/2001
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: The innate immune response protects against extracellular pathogens and utilizes pattern recognition receptors (PPRs), which in turn recognize molecularly conserved components known as pathogen-associated molecular patterns (PAMPs).  Heterophils are essential in establishing an innate immune response in neonatal chickens; however, the functional  activity and the mechanism(s) of action involved are not fully understood. The ability of either Salmonella enteritidis (SE) and lipopolysacharide (LPS) or Staphylococcus aureus (SA) and lipoteichoic acid (LTA) to promote degranulation and oxidative burst in heterophils isolated from neonatal chicks was evaluated.  Immune IgG opsonized SE was used as a positive control, which stimulates both activities through the Fc receptor. Treatment of heterophils with SE, LPS, SA, and LTA decreased degranulation activity by 76%, 78%, 90%, and 90%, respectively from the opsonized SE  control.  In addition, oxidative burst was evaluated using a quantitative luminol-dependent chemiluminescence assay.  SE, LPS, SA, and LTA all stimulated a significant oxidative burst in heterophils that was dramatically reduced by the addition of polyclonal antibody to Toll-like receptors 2 and 4.  Therefore, Toll-like receptors mediate oxidative burst but not degranulation, indicating at least two distinct pathways are involved in the function of heterophil killing.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov