U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Plant Science Research: St. Paul, MN
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area St. Paul, Minnesota Plant Science Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            ENDRE, GABRIELLA - UNIVERSITY OF MINNESOTAitem
                                            PENUELA, SILVIA - UNIVERSITY OF MINNESOTAitem
                                            KRISHNAMURTHY, NANDINI - TEXAS A&M UNIVERSITYitem
                                            Samac, Deborah

                                                         - *Debby*item
                                            VANDENBOSCH, KATHRYN - UNIVERSITY OF MINNESOTA
  Submitted to: International Congress on Molecular Plant-Microbe Interactions
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 7/11/2001
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: Medicago truncatula has become a useful model plant for molecular studies of nitrogen-fixing symbiotic interactions. This species is also very suitable as a model to investigate plant responses to foliar and root pathogens. As a basis for functional genomic analysis, 16 cDNA libraries have been constructed from different developmental states. Partial  sequencing of clones from these libraries has contributed to the > 120,000 ESTs from M. truncatula that have been deposited in GenBank by April 2001. Our long-term aims are to establish a unigene set of cDNA clones and to investigate thoroughly the genome-wide patterns of gene expression using hybridization of probes to DNA microarrays. As a first step, we have assembled a small scale array of ~1,000 cDNA clones ('kiloclone set') that contains positive and negative controls and clones of known tissue-specific patterns. The kiloclone set is also rich in clones encoding proteins with  putative functions in signal transduction, transcriptional regulation, control of cell division and cell death, pathogen response, secondary metabolism, and a number of genes of unknown function. Hybridization experiments to date have assessed differences in gene expression during the early steps of the symbiotic process or during pathogenic interactions, compared to uninoculated plants. Information on optimization of microarray production and hybridization and the latest results of this work, identifying differentially expressed clones, will be presented. We gratefully acknowledge support from the NSF Plant Genome Project, award number 9872664.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov