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Title:   Author  item
                                            Lillehoj, Hyunitem
                                            MIN, W - 1265-40-00item
                                            ZHU, J - 1265-40-00item
                                            ASHWELL, C - USDA, ARSitem
                                            VAN TASSELL, CURT - USDA, ARSitem
                                            Sonstegard, Taditem
                                            BURNSIDE, J - U DELAWARE, NEWARKitem
                                            MATTHEW, B - USDA, ARS
  Submitted to: Poultry Science Meeting
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 4/1/2001
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: Coccidiosis is an intestinal infection caused by intracellular protozoan parasites Eimeria. Understanding the interaction between host and parasites in the intestine is crucial in designing new approaches against this disease.  To better characterize host-parasite interactions, high-throughput gene  profiling DNA arrays were developed to identify chicken genes with altered expression following Eimeria infection. Over 1,000 expressed sequence tag (EST) genes from an activated T cell library were arrayed on DNA chips and hybridized with mRNAs from normal and E. acervulina infected intestinal cells. Approximately 200 genes showed significant changes. The success of this DNA microarray approach prompted us to construct a normalized cDNA library from intestinal epithelial cells and lymphocytes after  infection Eimeria to identify specific chicken genes regulating host protective immunity to coccidiosis. This library contains 1.87 x 107 transformants with an average insert size of 1.56 kb. Presently, over 6,000 clones have been EST sequenced of which 80% contain high quality inserts and 50% are unique based on a BLAST search. DNA microarray analysis of this library will allow better characterization of chicken genes involved in local host-parasite interactions in avian coccidiosis (Supported by Fund for Rural America, Grant No 9704985 and partially by ARS CRIS).


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