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Title:   Author  item
                                            Wise, Rogeritem
                                            Gobelman Werner, Karinitem
                                            HOLMES, JENNIFER - IOWA STATE UNIVERSITYitem
                                            DEQING, PEI - IOWA STATE UNIVERSITY
  Submitted to: Plant Genome Conference Proceedings
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 1/16/2002
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: T-cytoplasm maize (cms-T) has been developed as a model to study the molecular mechanisms underlying mitochondrial transcript processing and restoration of fertility in cytoplasmic male-sterile systems.  Male sterility in cms-T results from the function of T-urf13, a T-cytoplasm specific, mitochondrial gene.  Nuclear-directed modification of T-urf13  transcripts is mediated by Rf1, Rf8, or Rf*, which in combination with Rf2 suppress the male-sterile phenotype.  Rf1 is positioned near the centromere on chromosome 3, whereas Rf8 and Rf* are clustered together on 2L.  This position on 2L also harbours Rf3, another nuclear gene that modifies mitochondrial transcripts, but its action is specific to S-cytoplasm maize. Computational analysis of a candidate Rf1 cDNA was used to provide clues as to its possible function.  Twenty-six residues of the predicted amino acid sequence revealed 96% similarity with mammalian small nuclear  ribonucleoprotein E (snRNP E).  The snRNP E protein is an 11-kD basic protein integral to RNA processing reactions and is one of four core proteins associated with the snRNAs of the U (uridine rich) family (U1, U2, U4, U5, and U6).  U1 snRNA is initially bound to the 5'-splice site and is released upon recruitment of U4/U5/U6, a process that is consistent with the molecular phenotype of Rf1.  Research funded by USDA-NRI OEP 1999-01334.


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