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Title:   Author  item
                                            Kim, Jongitem
                                            Smith, Timothy

                                                         - *Tim*item
                                            Snelling, Warrenitem
                                            Vallet, Jeffitem
                                            Christenson, Ronald
  Submitted to: Animal Genetics
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 4/24/2003
                                    Publication  Date: 8/1/2003
                                    Citation: KIM, J.G., SMITH, T.P., SNELLING, W.M., VALLET, J.L., CHRISTENSON, R.K. BRIEF NOTE. LINKAGE MAPPING OF THE BOVINE BONE MORPHOGENETIC PROTEIN RECEPTOR-1B (BMPR1B) TO CHROMOSOME 6. ANIMAL GENETICS. 2003. v. 34(4). p. 311.Interpretive Summary: Genes encoding bone morphogenetic proteins (BMPs) and their receptors are involved in the function of reproductive organs including ovary and uterus, and in early fetal development of various species. A mutation in the ovine bone morphogenetic protein receptor IB (BMPR-IB) gene is associated with the increased ovulation rate and subsequent increased litter size phenotype in Booroola Merino sheep. Therefore, BMPR-IB is considered a candidate gene for ovulation rate in cattle. Recently, we have obtained cDNA for porcine BMPR-IB and mapped the gene in the porcine genome. Comparison of the ovine and porcine BMPR-IB cDNA sequences indicated that they are highly conserved and their amino acid sequences are 98.6% identical. Therefore, a pair of primers derived from the porcine BMPR-IB cDNA were used to amplify the bovine genomic DNA. An A/G single nucleotide polymorphism was detected in intron 8 (GenBank accession no. AY242067). This polymorphism was heterozygous in two of the four bulls from the MARC bovine reference population. The BMPR-IB gene was mapped to chromosome 6 position 42.4 cM. This gene may have implication on ovulation rate in cattle.
                                Technical Abstract: A mutation in the ovine bone morphogenetic protein receptor IB (BMPR-IB) gene is associated with an increased ovulation rate phenotype in Booroola Merino sheep. We have obtained cDNA for porcine BMPR-IB and mapped the gene in the porcine genome. Comparison of the ovine and porcine BMPR-IB cDNA sequences indicated they are highly conserved. Therefore, a pair of primers derived from the porcine BMPR-IB cDNA, which amplified across intron 8 in the pig, were used to amplify the bovine genomic DNA. Agarose gel electrophoresis and sequencing of the PCR amplicons of bovine genomic DNA indicated that the size of this product was 1253 bp (GenBank accession no. AY242067). Both strands of the amplified genomic DNAs were sequenced and evaluated for polymorphisms in 4 bulls from the Meat Animal Research Center (MARC) Bovine Reference Population. An A/G single nucleotide polymorphism was detected in intron 8 (GenBank accession no. AY242067), position 658 from the exon/intron boundary. This polymorphism was heterozygous in 2 of the 4 bulls from the MARC Bovine Reference Population. An assay was designed to genotype this polymorphism using primer extension with analyte detection on a MALDI-TOF mass spectrometer. This marker generated 50 informative meioses (40 phase known) in the MARC Bovine Reference Population. The BMPR-IB gene was mapped to chromosome 6 position 42.4 cM on the current MARC bovine chromosome 6 linkage map (http://www.marc.usda.gov/) using CRI-MAP near the QTL for milk production. The most significant two-point linkage detected was with BMS2508 (LOD=11.40) at 0.04 recombination. The BMPR-IB gene in human is located on chromosome 4q22.3, which shares homology with bovine chromosome 6.


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