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- Uniform Soybean Tests, Southern States

Title:   Author  item
                                            Ulloa, Mauricioitem
                                            Meredith Jr, William
  Submitted to: Crop Science Society Of America
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 11/5/2000
                                    Publication  Date: 11/5/2000
                                    Citation: ULLOA, M., MEREDITH JR, W.R. BULKED SEGREGANT ANALYSIS OF OKRA-LEAF AND NORMAL-LEAF COTTON NEAR-ISOLINES. CROP SCIENCE SOCIETY OF AMERICA. 2000. p. 182.Interpretive Summary:
                                Technical Abstract: Bulked segregant analysis with the use of near-isogenic lines identified unique segments of DNA among four near-isogenic morphological cotton leaf types (super-okra, sub-okra, okra, and normal).  The four genetically distinct leaf type isolines Gossypium hirsutum were assayed with approximately 530 molecular markers, SSRs and RAPDs.  A single 10-base  random oligonucleotide primer revealed polymorphic fragments among them. Each group of bulks had at least one different locus between leaf morphology types, and between groups.  Ten polymorphic fragments, which ranged from 0.5 to 2.0 Kb, will be isolated, cloned and sequenced.  The polymorphic fragments appear to be involved in cotton leaf morphology.


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