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Title:   Author  item
                                            WAALWIJK, CEES - PLANT RES INTL, NLitem
                                            LEE, THEO - PLANT RES INTL, NLitem
                                            HOWLETT, BARBARA - UNIV OF MELBOURNE, AUitem
                                            ARTS, JOOP - PLANT RES INTL, NLitem
                                            VRIES, INEKE - PLANT RES INTL, NLitem
                                            MENDES, ODETTE - PLANT RES INTL, NLitem
                                            HESSELINK, THAMARA - PLANT RES INTL, NLitem
                                            VERSTAPPEN, ELS - PLANT RES INTL, NLitem
                                            Goodwin, Stephen

                                                         - *Steve*item
                                            KEMA, GERT - PLANT RES INTL, NL
  Submitted to: Fungal Genetics Conference Proceedings
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 1/17/2002
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: Both mating type loci of Mycosphaerella graminicola were recently cloned and sequenced (WAALWIJK ET AL., FUNG. GENET & BIOL., in press). Within the non-idiomorphic sequences homology was found with palI, encoding a membrane receptor from Aspergillus nidulans, with apc, a putative component of the anaphase  promoting complex from Schizosaccharomyces pombe and with a DNA -(apurinic or apyrimidinic) lyase from S. pombe. Tblastn searches against the genomic sequence of N. crassa revealed that all of these homologs as well as the mat locus reside on the same contig, #2.82. Mating type genes are among the most widely studied genes in filamentous fungi and sequences of these genes as well as their flanking regions are available from many species, particularly from ascomycetes. We therefore selected  the mating type locus to perform comparative genomics among filamentous fungi and demonstrated, using recently developed bioinformatic tools, significant synteny between Fusarium spp., Leptosphaeria maculans, M. graminicola and Septoria passerinii. This shows that the genome sequence of Neurospora crassa greatly facilitates comparative genomics among ascomycetes.


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