U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 close



Research
                Research Home
                            National Programs
                            Research Projects
                            Scientific Manuscripts
                            International Engagement
                            Scientific Software/Models
                            Databases and Datasets
                            Office of Scientific Quality Review
            Media
                News & Features
                            Multimedia
                            Briefing Room
                            Down on the Farm
                            Press Room
                            Science in Your Shopping Cart
                            Scientific Discoveries
                            Tellus
                            Under the Microscope
            About ARS
                About ARS Home
                            Staff Directory
                            Labs and Research Centers (Map)
                            Headquarter Offices
                            Organizational Chart
                            Employee Services (REE Employees Only)
                            Office of Outreach, Diversity, and Equal Opportunity
            Work With Us
                Work With Us Home
                            ARS Vacancies at USAJOBS
                            Careers at ARS Information
                            Scientific Collaborations
                            Extramural Agreements
                            1890 Faculty Research Sabbatical ProgramSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Richards, Garyitem
                                            PARVEEN, SALINA - DELAWARE STATE UNIV
  Submitted to: Biochimica et Biophysica Acta
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 12/23/2004
                                    Publication  Date: 4/15/2005
                                    Citation: Richards, G.P., Parveen, S.A. 2005. A survey for phosphoglucose isomerase with lysyl aminopeptidase activity in vibrionaceae and non-vibrio pathogens. Biochimica et Biophysica Acta. 1748:128-133.Interpretive Summary: Previously, we identified a novel enzyme activity in Vibrio bacteria and determined a possible mechanism by which this enzyme may foster the spread of vibrios in infected individuals.  Since a variety of Vibrio species cause disease in humans and fish, a survey was conducted to determine the prevalence of this enzyme in other members of the Vibrio family and in non-Vibrio human pathogens.  This enzyme, known as phosphoglucose isomerase with a lysyl aminopeptidase activity (PGI-LysAP), was identified in chemically-purified cultures of all members from the Vibrio family.  No activity was detected in the non-vibrios including Salmonella, Shigella and E. coli.  Nutritionally rich culture media produced high levels of PGI-LysAP, suggesting that these bacteria, once in nutrient-rich tissues may produce more enzyme to enhance the invasiveness of vibrios in humans, fish, and other hosts.  Our findings may lead to the development of improved methods to identify vibrios in food, water, and clinical settings.
                                Technical Abstract: Phosphoglucose isomerase (PGI) with a novel lysyl aminopeptidase (LysAP) activity was recently purified and characterized from Vibrio vulnificus.  We showed that it cleaves the amino-terminal lysyl residue from des-Arg10-kallidin to produce des-Arg9-bradykinin, suggesting it plays a role in virulence.  A survey was conducted to determine the presence of this potential virulence-enhancing enzyme among twenty-three halotolerant human and fish pathogens from eleven species within the Vibrionaceae family, including V. vulnificus, V. parahaemolyticus, V. cholerae, Aeromonas hydrophila, and Plesiomonas shigelloides.  In addition, fourteen species of non-Vibrionaceae pathogens were screened for LysAP activity.  Cell lysates were partially purified by anion exchange chromatography and fractions were screened for LysAP and isomerase activities.  PGI-LysAP activity was detected in chromatographic fractions from all the Vibrionaceae, but was not detected in any of the non-Vibrionaceae pathogens.  Levels of isomerase and LysAP activity correlated (R2 = 0.92) for nine strains of V. vulnificus.  Since the Vibrionaceae represent an important family of human and fish pathogens, our identification of PGI-LysAP activity in a broad array of Vibrionaceae may lead to the development of improved analytical methods for their identification as well as interventions to reduce the high morbidity and mortality associated with some Vibrionaceae infections in clinical, veterinary, and aquaculture settings.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov