U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Crop Bioprotection Research: Peoria, IL
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    NCAUR HomepageSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area Peoria, Illinois National Center for Agricultural Utilization Research Crop Bioprotection Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Dowd, Patrickitem
                                            Johnson, Eric
  Submitted to: Journal of Agricultural and Food Chemistry
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 4/1/2005
                                    Publication  Date: 4/30/2005
                                    Citation: Dowd, P.F., Johnson, E.T. 2005. Association of a specific cationic peroxidase isozyme with maize stress and disease resistance responses, genetic identification and identification of a cDNA coding for the isozyme. Journal of Agricultural and Food Chemistry 53(11):4464-4470.Interpretive Summary: Fungal toxins (mycotoxins) in corn cause hundreds of millions of dollars in losses each year.  Both insects and fungi contribute to the problem.  A protein was identified that is associated with fungal resistance in several corn lines tested, and that also occurred in insect resistant material.  A cDNA clone that produced the protein was also identified.  Incorporation of the gene that produces the protein into corn through breeding or genetic engineering may result in more resistant material that accumulates lower levels of mycotoxins, thereby providing safer food for animals and people.
                                Technical Abstract: The presence of a pI 9.0 cationic peroxidase isozyme from milk stage pericarp of six susceptible and five resistant inbreds was correlated significantly with previously reported field data on percentage infection by Aspergillus flavus in the inbreds and their hybrids.  The isozyme was constitutively expressed in some additional maize tissues and lines examined, and frequently induced by mechanical damage, heat shock, Fusarium proliferatum, and/or Bacillus subtilis in other lines tested.  Native/IEF 2D electrophoresis identified the isozyme as the previously genetically identified px5.  A cDNA clone expressed in black Mexican sweet (BMS) maize cell cultures produced the pI 9.0 isozyme.  In addition to potential use in marker assisted breeding, enhanced expression of this cationic peroxidase through breeding or genetic engineering may lead to enhanced disease or insect resistance.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov