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ARS Home Northeast Area Beltsville, Maryland (BHNRC) Beltsville Human Nutrition Research Center Food Components and Health Laboratory Research Publications at this Location





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Title:   Author  item
                                            Novotny, Janetitem
                                            KURILICH, ANNE - DAIRY MANAGEMENTitem
                                            Britz, Stevenitem
                                            Clevidence, Beverly
  Submitted to: Carotenoid Gordon Conference
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 1/15/2004
                                    Publication  Date: 1/15/2004
                                    Citation: Novotny Dura, J., Kurilich, A.C., Britz, S.J., Clevidence, B.A. 2004. Labeled beta-carotene and lutein in plasma after consumption of isotopically-labeled kale. Carotenoid Gordon Conference.Interpretive Summary: none
                                Technical Abstract: To investigate the bioavailability of carotenoids from a green, leafy vegetable, isotopically-labeled kale was produced and used in a human feeding trial.  Kale was chosen because of its high content of both beta-carotene and lutein.   The kale was grown in a sealed chamber in the presence of 13C-carbon dioxide (99% enrichment) to produce fully labeled plants.  After harvest, the blanched kale leaves (400 grams as a single dose) were ingested by volunteers with 30 grams of safflower oil.  The kale dose supplied approximately 20 mg of both beta-carotene and lutein, both of which contained greater than 98% 13C.  Serial plasma samples were collected for 4 weeks following the dose.  Plasma 13C-beta-carotene, 13C-lutein, and 13C-retinol were determined by LC-MS.  The concentration of 13C-lutein in plasma of subjects was substantially greater than that of 13C-beta-carotene.  In addition, the 13C-lutein observed in subjects' plasma represented a greater proportion of the endogenous plasma pool that did the 13C-beta-carotene. The isotopic labeling of kale combined with its use in a human feeding trial has provided a new way to examine the comparative bioavailability of beta-carotene and lutein from green, leafy vegetables.


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