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Title:   Author  item
                                            OKAMURA, M - US FOOD & DRUG ADMINitem
                                            Lillehoj, Hyunitem
                                            RAYBOURNE, RICHARD - US FOOD & DRUG ADMINitem
                                            BABU, UMA - US FOOD & DRUG ADMINitem
                                            HECKERT, ROBERT - UNIV OF MARYLAND
  Submitted to: American Association of Avian Pathologists
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 7/1/2003
                                    Publication  Date: 9/4/2003
                                    Citation: Okamura, M., Lillehoj, H.S., Raybourne, R., Babu, U., Heckert, R. 2003. Antigen-specific lymphocyte proliferation, interleukin-6 (il-6)and il-2 production in chickens immunized with outer membrane protein of salmonella enteritidis.[abstract] American Association of Avian Pathologists. Paper No. 163.Interpretive Summary:
                                Technical Abstract: The immunogenic effect of outer membrane protein (OMP) of Salmonella enteritidis (SE) in inducing cell-mediated immune response was studied. White leghorn inbred chickens were subcutaneously immunized with OMP or crude protein (CP) from SE, or a commercially SE killed vaccine twice at 6 and 9 wk of age. Proliferation responses of spleen lymphocytes to concanavalin A (Con A), OMP, CP, flagella, porin and lipopolysaccharide (LPS) antigens from SE were evaluated in vitro.  Kinetics of serum interleukin (IL)-6 and IL-2 production were evaluated by an indirect enzyme-linked immunosorbent assay (ELISA) at 1, 2, 3, 4, and 5 wk after primary immunization with SE antigens or a SE killed vaccine. OMP- and CP-immunized chickens as well as chickens immunized with a commercial killed vaccine showed a good proliferation response at 4 weeks post primary immunization compared to the control groups which were injected with saline or adjuvant alone.  SE flagella antigen was significantly more effective (P < 0.05) in stimulating lymphocyte proliferation in vitro than other antigens. Compared to the control groups, chickens which were immunized with OMP, CP, or killed vaccine produced a significantly higher levels of serum IL-6 and IL-2 (P < 0.05) after primary immunization.  These results indicate that a subcutaneous immunization with the killed vaccine, bacterial CP or OMP is capable of eliciting cell-mediated immunity which might be associated with protective immune response to SE.


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