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Title:   Author  item
                                            Lillehoj, Hyunitem
                                            CHOI, K - IDRL, ANRI, ARSitem
                                            WIN, W - IDRL, ANRI, ARSitem
                                            BABU, U - FDAitem
                                            BURNSIDE, J - UNIV. OF DELAWAREitem
                                            MIYAMOTO, T - IDRL, ANRI, ARS
  Submitted to: Avian Immunology Research Group Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 5/28/2001
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: Full-length cDNA encoding chicken IL-15 designated as pc3.1/5101, was isolated from chicken liver cDNA library and additional sequences were obtained using 5' rapid amplification of cDNA ends. The full-length chicken IL-15 cDNA contains an open reading frame of 187 amino acids with a single potential N-linked glycosylation site.  The predicted protein sequence  shows similarities of 55%, 53% and 52% with mouse, human and bovine IL-15 sequences, respectively.  The pc3.1/5101 has total 699 bp including the 561 bp open reading frame with a predicted m.w. of  21, 964 kDa.  Chicken IL-15 contains all 4 highly conserved cysteine residues present in mammalian IL- 15 sequences, including 2 upstream AUGs of the 5' UTR. Recombinant chicken IL-15 (rchIL-15) was expressed in prokaryotic and eukaryotic expression systems and their tissue distribution, molecular, biochemical and functional characterizations were compared to the chicken IL-2.  Northern  blotting analysis demonstrated that the chicken IL-15 gene is expressed in many tissues including liver, cecal tonsil, muscle, heart, intestine and spleen whereas IL-2 was only expressed in the concanavalin-A (Con A)- stimulated spleen cells.  Collectively, these results indicate that the pc3.1/5101 gene represents an avian homologue of mammalian IL-15.


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