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Title:   Author  item
                                            Min, Wongiitem
                                            Lillehoj, Hyunitem
                                            KIM, SUNGWON - USDA-ARS,PBESLitem
                                            Zhu, Jamesitem
                                            Beard, Hunteritem
                                            ALKHAROUF, NADIM - USDA-ARS,PSIitem
                                            MATTHEWS, BENJAMIN - USDA-ARS,PSI
  Submitted to: American Association of Avian Pathologists
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 6/1/2003
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract:       Avian coccidiosis is caused by several Eimeria strains which infect different regions of the intestine inducing a strain-specific immunity. To investigate host immune response against two major Eimeria spp., we infected SC White-Leghorn chickens with E. acervulina and E. maxima and compared their global transcriptional changes elicited in the intestinal intraepithelial lymphocytes (IELs) using nylon and glass cDNA microarrays.  Initially, nylon microarray containing 2600 EST cDNAs selected from the activated T-lymphocyte and macrophage cDNA libraries (http://www.chickest.del.edu) were  hybridized with mRNAs extracted from the IELs obtained from E. acervulina- or E. maxima-infected chickens.  On the basis of significant changes associated with infection, 419 clones showing at least 1.5-fold changes over the uninfected controls, were selected for cDNA microarray on a glass slide and local host response to Eimeria infection was analyzed at four different time points after primary and secondary infections. In general, E. acervulina infection resulted in greater changes in the local gene expression than E. maxima infection. Both E. acervulina and E. maxima infections induced 37 and 10 genes in the primary and  secondary infections, respectively, while repressed 43 and 10 genes in the primary and secondary infections, indicating that the primary infections resulted in a greater change than the secondary infection. The detailed analysis of the local changes in host gene expression following Eimeria infection will enhance our understanding of host-parasite immunobiology leading to protective immunity.


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