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Title:   Author  item
                                            Dubey, Jitenderitem
                                            LIDDELL, S - PBELitem
                                            MATTISON, D - OREGON STATE UNIVERSITYitem
                                            SPEER, C - DEPT OF VET MOLE BIOitem
                                            HOWE, D - UNIVERSTIY OF KENTUCKYitem
                                            Jenkins, Mark
  Submitted to: Systematic Parasitology
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 10/10/2000
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary: Neospora hughesi is a recently discovered single-celled parasite.  It can cause a neurological disease in horses.  Little is known of the biology of this organism.  Scientists at the Beltsville Agricultural Research Center and the Oregon State University report characteristics of an isolate of Neospora hughesi recovered from the spinal cord of a horse with neurologic  signs.  Results will be of interest to biologists, parasitologist and pathologists.
                                Technical Abstract: Neospora organisms were isolate in cell cultures inoculated with homogenate of spinal cord from a horse in Oregon.  Tachyzoites of this Oregon isolate of Neospora were maintained continuously by cell culture passage and tachyzoites were infective to immunosuppressed mice.  Gamma interferon gene knockout (KO) mice injected with tachyzoites developed fatal myocarditis  and numerous tachyzoites were seen in lesions.  Gerbils (Meriones unguiculatus) inoculated with tachyzoites developed antibodies (>1:500) as indicated by the N. caninum aggultination test, but did not develop clinical signs and Neospora was not demonstrable in their tissues.  Tissue cysts were not found in gerbils, nude mice, KO mice, cortisonized outbred Swiss Webster mice, or BALB/c mice injected with the Oregon equine isolate of Neospora.  Ultrastructurally, tachyzoites of the Oregon isolate from the myocardium of infected KO mice and from cell culture were similar to N.  caninum tachyzoites.  Western blot analysis using SAG1 and SRS2 polyclonal and monoclonal antibodies, and characterization of the internal transcribed spacer 1(ITS1) sequences from the equine isolates and different isolates of Neospora from dogs and cattle, indicated that the equine isolates of Neospora, including the Oregon isolate, are distinct from N. caninum isolates from cattle and dogs.


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