U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 close



Research
                Research Home
                            National Programs
                            Research Projects
                            Scientific Manuscripts
                            International Engagement
                            Scientific Software/Models
                            Databases and Datasets
                            Office of Scientific Quality Review
            Media
                News & Features
                            Multimedia
                            Briefing Room
                            Down on the Farm
                            Press Room
                            Science in Your Shopping Cart
                            Scientific Discoveries
                            Tellus
                            Under the Microscope
            About ARS
                About ARS Home
                            Staff Directory
                            Labs and Research Centers (Map)
                            Headquarter Offices
                            Organizational Chart
                            Employee Services (REE Employees Only)
                            Office of Outreach, Diversity, and Equal Opportunity
            Work With Us
                Work With Us Home
                            ARS Vacancies at USAJOBS
                            Careers at ARS Information
                            Scientific Collaborations
                            Extramural Agreements
                            1890 Faculty Research Sabbatical ProgramSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Alvarez, Reneitem
                                            LWAMBA, HUMPHREY - UNIV OF MN-ST PAUL, MNitem
                                            Kapczynski, Darrellitem
                                            NJENGA, M KARIUKI - UNIV OF MN-ST PAUL, MNitem
                                            Seal, Bruce
  Submitted to: American Society for Virology Meeting
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 5/1/2003
                                    Publication  Date: 7/10/2003
                                    Citation: Alvarez, R., Lwamba, H.M., Kapczynski, D.R., Njenga, M., Seal, B.S. 2003. Nucleotide and predicted amino acid sequence analysis of the avian metapneumovirus type c cell attachment glycoprotein (g) gene.  phylogenetic analysis among the pneumovirinae and molecular epidemiology of u.s. viruses. American Society for Virology Meeting.Interpretive Summary:
                                Technical Abstract: A serologically distinct avian metapneumovirus (aMPV) was isolated in the U.S. following an outbreak of turkey rhinotracheitis (TRT) during February of 1997.  The newly recognized U.S. virus was subsequently demonstrated to be genetically distinct from European subtypes and was designated aMPV/C.  We have determined gene nucleotide and predicted amino acid sequence of the cell attachment (G) glycoprotein of aMPV type C (aMPV/C; Colorado and three Minnesota isolates) by sequencing cloned cDNAs synthesized from intracellular RNA of aMPV/C infected cells.  The nucleotide sequence comprised 1321 nucleotides with only one predicted open reading frame encoding a protein of 435 amino acids, with a predicted Mr 48,840.  Structurally, the predicted G protein of aMPV/C maintained similar characteristics to the human respiratory syncytial virus (hRSV) attachment G protein, including two mucin-like regions (heparin-binding domains) flanking both sides of a CX3C chemokine motif present in a conserved hydrophobic pocket.  Comparison of the deduced G protein sequence of aMPV/C with that of aMPV types A, B, and D, as well as hRSV revealed overall predicted amino acid sequence identities ranging from 4 to 16.5%, suggesting a distant relationship.  However, G protein sequence identities ranged from 72% to 97% when aMPV/C was compared to other members within the aMPV/C subtype or 21% for the recently identified human metapneumovirus (hMPV) G protein.  Ratios of non-synonymous to synonymous nucleotide changes were greater than one in the G gene when comparing the more recent Minnesota isolates to the original Colorado isolate.  Epidemiologically this suggests some form of positive selection among U.S. isolates since the first outbreak of TRT in the U.S.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov