U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 close



Research
                Research Home
                            National Programs
                            Research Projects
                            Scientific Manuscripts
                            International Engagement
                            Scientific Software/Models
                            Databases and Datasets
                            Office of Scientific Quality Review
            Media
                News & Features
                            Multimedia
                            Briefing Room
                            Down on the Farm
                            Press Room
                            Science in Your Shopping Cart
                            Scientific Discoveries
                            Tellus
                            Under the Microscope
            About ARS
                About ARS Home
                            Staff Directory
                            Labs and Research Centers (Map)
                            Headquarter Offices
                            Organizational Chart
                            Employee Services (REE Employees Only)
                            Office of Outreach, Diversity, and Equal Opportunity
            Work With Us
                Work With Us Home
                            ARS Vacancies at USAJOBS
                            Careers at ARS Information
                            Scientific Collaborations
                            Extramural Agreements
                            1890 Faculty Research Sabbatical ProgramSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            KIM, J - CHANGWON NATIONAL UNIV.item
                                            Lillehoj, Hyunitem
                                            MIN, W - IDRL, ANRI, ARSitem
                                            HAN, J - SEOUL NATIONAL UNIVERISTYitem
                                            SASAI, K - OSAKA PREFECTURE UNIV.item
                                            SOHN, E - CHANGWON NATIONAL UNIV.item
                                            LILLEHOJ, E - UNIVERSITY OF MARYLAND
  Submitted to: Avian Immunology Research Group Abstract
                                    Publication Type: Proceedings
                                    Publication Acceptance Date: 4/1/2001
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary: Chicken coccidiosis is caused by Eimeria species which are intracellular parasites. These parasites infect the host intestine causing severe intestinal damage.  Chickens infected with Eimeria are unable to grow normally due to poor nutrient absorption by the damaged intestine. Currently, prophylactic medication is a major method to control  coccidiosis.  However, due to increasing incidence of drug-resistance of these parasites, new strategy to control coccidiosis is needed.  In this report, ARS scientists have genetically engineered recombinant chicken antibody molecules which are specific for Eimeria parasite proteins.  These recombinant chicken antibodies will be used to block parasite invasion and to reduce economic loss due to coccidiosis.
                                Technical Abstract: Chicken hybridomas secreting monoclonal antibodies against Eimeria acervulina sporozoites were produced to identify parasite proteins involved in host cell invasion.  While most antibodies recognized uncharacterized parasite surface proteins, two were identified with reactivity against apical complex proteins located at the anterior tip of sporozoites.  To  express high concentration of these antibodies for potential application a immunotherapeutic reagents, a single chain fragment variable (ScFv) region gene was constructed by PCR amplification of the variable heavy (VH) and light (VL) chain genes with a flexible linker.  By indirect immunofluorescence, recombinant ScFv antibody expressed in E. coli showed identical binding specificity as the original monoclonal antibody against E. acervulina and E. tenella sporozoites, staining the apical complex and thus demonstrating reactivity against native parasite protein.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov