U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 close



Research
                Research Home
                            National Programs
                            Research Projects
                            Scientific Manuscripts
                            International Engagement
                            Scientific Software/Models
                            Databases and Datasets
                            Office of Scientific Quality Review
            Media
                News & Features
                            Multimedia
                            Briefing Room
                            Down on the Farm
                            Press Room
                            Science in Your Shopping Cart
                            Scientific Discoveries
                            Tellus
                            Under the Microscope
            About ARS
                About ARS Home
                            Staff Directory
                            Labs and Research Centers (Map)
                            Headquarter Offices
                            Organizational Chart
                            Employee Services (REE Employees Only)
                            Office of Outreach, Diversity, and Equal Opportunity
            Work With Us
                Work With Us Home
                            ARS Vacancies at USAJOBS
                            Careers at ARS Information
                            Scientific Collaborations
                            Extramural Agreements
                            1890 Faculty Research Sabbatical ProgramSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            CANADA, NUNO - UNIV. PORTOitem
                                            MEIRELES, CARLA - UNIV. PORTOitem
                                            ROCHA, ANTONIO - UNIV. PORTOitem
                                            SOUSA, SUSANA - CTR. PARASITE IMM. BIO.item
                                            THOMPSON, GERTRUDE - UNIV. PORTOitem
                                            Dubey, Jitenderitem
                                            ROMAND, S - INST. PUERICULTUREitem
                                            THULLIEZ, P - INST. PUERICULTUREitem
                                            CORREIA DA COSTA, J - UNIV. PORTO
  Submitted to: Veterinary Parasitology
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 11/30/2002
                                    Publication  Date: 12/20/2002
                                    Citation: Canada, N., Meireles, C., Rocha, A., Sousa, S., Thompson, G., Dubey, J.P., Romand, S., Thulliez, P., Correia Da Costa, J. 2002. First portugese isolate of neospora caninum from an aborted fetus from a dairy herd with endemic neosporosis. Veterinary Parasitology 110:11-15.Interpretive Summary: Neospora caninum is a single celled parasite of livestock and companion animals.  It causes abortion in livestock and paralysis in companion animals.  Abortion caused by N. caninum (neosporosis) have been reported worldwide.  Scientists at the Beltsville Agricultural Research Center and University of Porto, Portugal report for the first time isolation of N. caninum from an aborted dairy cow from Portugal.  These findings will be of interest to biologists, Veterinarians, and parasitologists.
                                Technical Abstract: Neospora caninum was isolated from the brain of an aborted 4 month-old fetus from a dairy cow herd with endemic neosporosis in Porto, Portugal.  The fetal brain homogenate was inoculated interperitoneally first into outbred Swiss Webster mice given dexamethasone and then the peritoneal exudates from these mice was co-inoculated with mouse sarcoma cells in the peritoneal cavity of mice given dexamethasone.  Neospora caninum tachyzoites were seen in peritoneal exudate of the second passage.  Tachyzoites from the peritoneal exudate reacted positively with anti-N. caninum antibodies and not with anti-Toxoplasma gondii antibodies and contained N. caninum specific DNA.  This Portuguese isolate of N. caninum has been successfully maintained in cell culture.  The dam of the aborted fetus had an antibody titer of 1:10240 in the Neospora agglutination test.  Antibodies to N. caninum were found in 76 of 106 cows from this herd in titers of 1:40 in 31, 1:80 in 22, ¿1:160 or more in 23 in the Neospora agglutination test.  This is the first isolation of a viable N. caninum-like parasite from any host in Portugal.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov