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Title:   Author  item
                                            Fravel, Deborahitem
                                            Bailey, Bryanitem
                                            BAO, J. - FORMER ARS
  Submitted to: Proceedings of Methyl Bromide Alternatives Conference
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 11/6/2002
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary: N/A
                                Technical Abstract: Differences in gene expression in Fusarium oxysporum were studied using differential display analysis.  RNA was extracted from two isolates identified as pathogens and two isolates with high biocontrol ability.  First strand cDNA was amplified from total RNA by reverse transcription.  The cDNA was amplified again using anchored and random primers and the resulting products were separated by gel electrophoresis.  The fragments were visualized using a flourescent scanner.  Twenty-two cDNA bands with potential differential expression between the pathogenic and biocontrol strains were identified.  These bands were excised and the cDNAs sequenced.  Bands showing high homology to known sequences included an ATP synthase, an ABC transporter, and a lysine permease. ABC transporters can function in detoxification of toxic compounds and have not been previously reported in Fusarium. Studies are currently underway with additional pathogenic, biocontrol and saprophytic strains to determine whether these genes are present in the different phenotyes (but expressed differently), as well as studies on the regulation of the ABC transporter.


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