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Title:   Author  item
                                            Grabber, Johnitem
                                            Hatfield, Ronalditem
                                            Ralph, John
  Submitted to: American Society of Agronomy Meetings
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 11/9/2000
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: Nonlignified (NL) walls from cell suspensions of Zea mays (L.) were treated with pectin methylesterase (PME) and then synthetically lignified (SL) with coniferyl alcohol to assess whether uronate methyl esters and benzyl-uronate cross-links restrict pectin and cell wall degradation by fungal enzymes. PME treatment of walls reduced uronic acid concentrations  from 110 to 90 g/kg and methyl esterification of uronic acids from 51 to 20%. Klason lignin concentrations in SL walls were, however, increased from 99 to 116 g/kg by PME treatment. The release of uronic acids after 4- and 72-h of enzymatic hydrolysis were 58 and 76% for NL walls, 68 and 94% for PME-NL walls, 44 and 79% for SL walls and 23 and 86% for PME-SL walls, respectively.  These results indicate that methyl esters restrict both the rate and extent of pectin degradation. Demethylation of uronic acids, however, enhanced the formation of benzyl-uronate cross-links in  SL walls and these cross-links limited the rate but not the extent of pectin degradation. Lignification reduced the release of total sugars from 468 to 144 g/kg after 4 h and from 771 to 510 g/kg after 72 h of enzymatic hydrolysis. Pectin methylation and benzyl-uronate cross-linking did not influence the release of total sugars from NL or SL walls during enzymatic hydrolysis.


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