U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 close



Research
                Research Home
                            National Programs
                            Research Projects
                            Scientific Manuscripts
                            International Engagement
                            Scientific Software/Models
                            Databases and Datasets
                            Office of Scientific Quality Review
            Media
                News & Features
                            Multimedia
                            Briefing Room
                            Down on the Farm
                            Press Room
                            Science in Your Shopping Cart
                            Scientific Discoveries
                            Tellus
                            Under the Microscope
            About ARS
                About ARS Home
                            Staff Directory
                            Labs and Research Centers (Map)
                            Headquarter Offices
                            Organizational Chart
                            Employee Services (REE Employees Only)
                            Office of Outreach, Diversity, and Equal Opportunity
            Work With Us
                Work With Us Home
                            ARS Vacancies at USAJOBS
                            Careers at ARS Information
                            Scientific Collaborations
                            Extramural Agreements
                            1890 Faculty Research Sabbatical ProgramSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Hill, Doloresitem
                                            SCOTT, ALAN - JOHNS HOPKINS, BALTO, MDitem
                                            DYER, ROBERT - UNIV DELAWARE, NEWARKitem
                                            Dubey, Jitender
  Submitted to: Meeting Abstract
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 6/15/2001
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: Vertical transmission of Neospora caninum has been documented in cattle, though the fidelity of transmission from dam to offspring is variable.  The development of resistance to Neospora infection and transmission of N. caninum from dams to offspring may be affected by immunological recognition of specific N. caninum  proteins by infected cattle.  In this study, Neospora caninum tachyzoites were isolated from in vitro cell culture and proteins were extracted using a chaotropic detergent solution.  Two- dimensional electrophoresis was carried out on the solubilized proteins using immobilized pH gradient (3-10) strips in the first dimension and 4-12% gradient slab gels in the second dimension. XProteins identified in Western blots by antisera from infected cows were cut from identical blots run in parallel and were  subjected to N-terminal amino acid sequence analysis for identification.  Twenty  immunodominant proteins were identified using  sera from N. caninum infected cattle.  Four of the proteins were identified as surface associated proteins, 3 were identified as dense granule-associated proteins, 4 were identified as actin, myosin, or tubulin chains,  2 were identified as microneme associated proteins, and 3 were identified as cytoplasmic proteins.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov