U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Food and Feed Safety Research: College Station, TX
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Plains Area College Station, Texas Southern Plains Agricultural Research Center Food and Feed Safety Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Sheffield, Cynthiaitem
                                            Hume, Michaelitem
                                            Droleskey, Robert

                                                         - *Bob*item
                                            Harvey, Rogeritem
                                            Bischoff, Kenneth
  Submitted to: Online Journal of Veterinary Research
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 4/2/2003
                                    Publication  Date: 5/22/2003
                                    Citation: SHEFFIELD, C.L., HUME, M.E., DROLESKEY, R.E., HARVEY, R.B., BISCHOFF, K.M. RIBOTYPE CHARACTERIZATION AND ANTIMICROBIAL SUSCEPTIBILITY PROFILES OF CAMPYLOBACTER COLI ISOLATES FROM SWINE. Online Journal of Veterinary Research. 2003. v. 7. p. 52-58.Interpretive Summary: Campylobacter, a bacteria commonly found in pigs, is the cause of an estimated 2.5 million cases of human foodborne illness each year.  In this study, a small number of Campylobacter was collected from the feces of adult, female pigs and 21 day old piglets, which were housed in the same commercial production barn.  Some of the piglets were the offspring of the adult female pigs.  The bacterial samples were analyzed for their individual genetic makeup by an automated system called a RiboPrinter **R.  The resulting ribotype analysis was used to determine if the bacteria were related genetically.  Sixty-two percent of the pigs were found to have unrelated Campylobacter in their feces, and the adult female pigs did not share any Campylobacter of an identical genetic makeup with their offspring.  However, genetically identical Campylobacter were found in the offspring in two sets of littermates. The collected bacteria were also tested to determine if specific chemical (antibacterial) agents could be used to kill the bacteria.  All of the Campylobacter collected could be killed by the agent, Erythromycin, but none of the bacteria could be killed by the agents, Lincomycin and Tobramycin.  This study shows that many genetically unrelated Campylobacter can be found at a single pig barn.  Since Campylobacter represents a serious threat to the health of all consumers, it would be worth while to gain a more complete picture of the Campylobacter present in commercial production barns by collection and analysis of a larger number of samples.
                                Technical Abstract: Objective:  To evaluate the genetic relatedness and antibiotic sensitivity profiles of Campylobacter coli isolates from sows and piglets housed in an integrated swine production facility.
Sample Population:  Ninety-nine isolates of Campylobacter coli were collected from 3 sows (Yorkshire-Landrace) and 18 piglets (Yorkshire-Landrace X Duroc or Hampshire) housed in a common farrowing barn.
Procedure:  When piglets were weaned (21 day of age) fecal samples were collected for the sows and rectal samples were collected from the piglets.  Isolation of Campylobacter coli was performed using an enrichment broth and restrictive media under microaerophilic conditions.
Results:  The Campylobacter coli isolates segregated into 20 ribogroups and exhibited 32 antibiotic susceptibility profiles.   The Ribogroup (224-373-S-5) contained 35 isolates from eleven animals.  Thirty-eight percent of the animals exhibited a single ribogroup, while 10% of the animals exhibited four ribogroups.  No discernible pattern of ribogroup relatedness was observed among the sows and piglets or among littermates.
Conclusion:  The data suggests a high level of diversity in both ribotypic patterns and antibiotic sensitivity profiles among the Campylobacter coli isolated from related pigs housed in a single facility.  Further, no evidence was found for a direct transfer of specific Campylobacter coli ribotypes from a sow to her piglets.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov