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Title:   Author  item
                                            Kogut, Michael

                                                         - *Mike*item
                                            Crippen, Tawni

                                                         - *Tc*item
                                            LOWRY, VIRGINIA - TEXAS A&M UNIVERSITYitem
                                            FARNELL, MORGAN - TEXAS A&M UNIVERSITY
  Submitted to: Veterinary Immunology International Symposium
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 7/15/2001
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: Fc receptors of avian heterophils play a primary role in the elimination of bacterial pathogens in poultry.  The cross-linking of Fc receptors with bacteria-Ig complexes results in the secretion of toxic oxygen metabolites and anti-bacterial granules.  We have been investigating the upstream signaling events that precede degranulation by heterophils.  Using the  non-specific pharmacological inhibitors genistein, chelerythrine, and staurosporin, we found no significant inhibitory effects on Fc-mediated heterophil degranulation.  In these studies, we used more selective pharmacological inhibitors to investigate the roles of mitogen activated protein kinases, tyrosine kinases, phospholipases, phosphatidylinositol 3'-kinase, and intracellular calcium mobilization on Fc-mediated heterophil degranulation.  Neither PP2, a selective inhibitor of the Src family of protein tyrosine kinases, nor piceatannol, a selective inhibitor  of Syk tyrosine kinase, had inhibitory effects on degranulation.  However, Fc-mediated degranulation was significantly attenuated by SB 203580, an inhibitor of p38 MAPK, U73122, an inhibitor of phospholipase C, and LY294002, an inhibitor of PI3-kinase.  Lastly, chelation of extracellular calcium by EGTA did not affect degranulation whereas chelation of intracellular calcium by BAPTA attenuated this response.  These results suggest that p38 MAPK, phospholipase C, PI3-K, and calcium mobilization participate in the signaling pathways that regulate Fc-receptor-mediated degranulation of chicken heterophils.


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