U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Infectious Bacterial Diseases Research: Ames, IA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area Ames, Iowa National Animal Disease Center Infectious Bacterial Diseases Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            LYASCHENKO, K - CHEMBIOitem
                                            GREEENWALD, R - CHEMBIOitem
                                            ESFANDIARI, J - CHEMBIOitem
                                            Pollock, Jessicaitem
                                            ANDERSEN, P - COPENHAGEN, DENMARKitem
                                            Palmer, Mitchellitem
                                            Waters, Wade
  Submitted to: Veterinary Immunology International Symposium
                                    Publication Type: Proceedings
                                    Publication Acceptance Date: 5/1/2004
                                    Publication  Date: 7/25/2004
                                    Citation: Lyaschenko, K., Greeenwald, R., Esfandiari, J., Pollock, J.J., Andersen, P., Palmer, M.V., Waters, W.R. 2004. Antigen recognition in white-tailed deer experimentally infected with mycobacterium bovis [abstract]. Veterinary Immunology International Symposium. Paper No. WK.11.6.7:369.Interpretive Summary:
                                Technical Abstract: White-tailed deer (Odocoileus virginianus) have recently emerged as wildlife reservoirs of Mycobacterium bovis infection of cattle in Northern America. To improve control of bovine tuberculosis new diagnostics for M. bovis infection in white-tailed deer are therefore needed. The goal of the present study was to characterize the humoral immune responses in deer experimentally infected with M. bovis. Animals were infected with various doses of M. bovis either by aerosol, or intratonsilarly, or by exposure to already infected deer. Serum samples were sequentially collected during up to 10 months post-infection. Antibodies were detected in serum samples by MAPIA (MultiAntigen Print ImmunoAssay) using a panel of 12 native and recombinant antigens of M. bovis. We found that 3 of 6 animals in the low-dose aerosol-infected group produced antibodies to one or more recombinant proteins. Positive correlation was observed between the humoral immune responses and pathology developed in the infected deer. In fact, animals with most advanced disease demonstrated the strongest MAPIA reactions involving the greatest number of antigens. Further, only 1 out of 3 seronegative deer in this group showed signs of disease.  In the high-dose intratonsilar infection group, 10 out of 11 animals developed specific IgG antibodies against one or more recombinant antigens (all 11 reacted with M. bovis culture filtrates). The humoral responses were boosted significantly one month after intradermal tuberculin skin testing. All 'in-contact' infected deer (8/8) produced antibody responses against multiple antigens. Importantly, the magnitude of these responses was comparable to that of animals inoculated with M. bovis. Patterns of antigen recognition varied from animal to animal in each group, although similar patterns could be found in deer infected by different routes. Protein MPB83 appeared to be the most immunodominat serological target in the experimental M. bovis infection. However, additional seroreactive antigens might be required for developing a sensitive serodiagnostic test for deer tuberculosis


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov