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Title:   Author  item
                                            Adhikari, Tikaitem
                                            BALAJI, BOOVARAGHAN - PURDUE UNIVERSITYitem
                                            Breeden, Jillitem
                                            Crane, Charlesitem
                                            Anderson, Josephitem
                                            Goodwin, Stephen

                                                         - *Steve*
  Submitted to: Phytopathology
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 7/31/2004
                                    Publication  Date: 7/31/2004
                                    Citation: Adhikari, T., Balaji, B., Breeden, J.D., Crane, C.F., Anderson, J.M., Goodwin, S.B. 2004. Real-time PCR analysis of genes expressed during wheat-mycosphaerella graminicola interactions. Phytopathology. 94:S2-S3.Interpretive Summary:
                                Technical Abstract: We are using genomics tools to identify genes involved in disease resistance. As a first step, we utilized results from a large-scale cDNA-AFLP profiling experiment in wheat to determine global gene-expression responses against two fungi, two viruses and Hessian fly. This analysis identified many genes with differential expression specific to each interaction. We used the interaction between wheat and the fungus Mycosphaerella graminicola to further test the hypothesis that many of the host genes showed altered expression in response to infection. Thirty up-regulated genes, such as phenylalanine ammonia lyase, heat-stress genes, transcription factors, peroxidases, etc., were selected for the study. Expression analysis of these genes by quantitative real-time PCR confirmed differential expression in the resistant and susceptible wheat cultivars at several time points after infection. A summary of the findings will be discussed.


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