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Title:   Author  item
                                            Dubey, Jitenderitem
                                            LINDSAY, D - VIRGINIA TECHitem
                                            Hill, Doloresitem
                                            ROMAND, S - INST. DE PUERCULTUREitem
                                            THULLIEZ, P - INST. DE PUERCULTUREitem
                                            Kwok, Oliveritem
                                            SILVA, J - UNIV. SAO PAULOitem
                                            OLIVEIRA-CAMARGO, M - CENT. CONTR. ZOOitem
                                            GENNARI, S - UNIV. SAO PAULO
  Submitted to: Journal of Parasitology
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 7/10/2002
                                    Publication  Date: 8/10/2002
                                    Citation: Dubey, J.P., Lindsay, D.S., Hill, D.E., Romand, S., Thulliez, P., Kwok, O.C., Silva, J., Oliveira-Camargo, M.C., Gennari, S.M. 2002. Prevalence of antibodies to neospora caninum and sarcocystis neurona in sera of domestic cats from brazil. Journal of Parasitology 88:1251-1252.Interpretive Summary: Neospora caninum is a single-celled parasite of livestock and companion animals.  It causes abortion in livestock and paralysis in companion animals.  Dogs are the reservoirs of its infection because they are the only animals that can excrete environmentally-resistant oocysts.  Scientists at the Beltsville Agricultural Research Center and the University of Sao Paulo, Brazil report for the first time antibodies to N. caninum in 11.9% of 502 cats from the city of Sao Paulo.  These results will be of interest to veterinarians, biologists, and parasitologists.
                                Technical Abstract: Antibodies to Neospora caninum and Sarcocystis neurona were determined in serum samples of 502 domestic cats from Brazil using direct agglutination tests employing the respective antigens.  Antibodies to S. neurona were not found in 1:50 dilution of any serum in the S. neurona agglutination test, suggesting that domestic cats from São Paulo city were not exposed to S. neurona sporocysts from opossums.  Antibodies to N. caninum were found in 60 (11.9%) of 502 cats with titers of 1:40 in 36 cats, 1:80 in 18 cats, 1:160 in 5 cats and 1:800 in 1 cat using the Neospora agglutination test (NAT).  Antibodies to N. caninum were confirmed by Western blotting in the sera of 10 cats with 1:80 to 1:800 NAT titer; this finding suggests that at least 10 cats had N. caninum-specific antibodies confirmed by 2 tests.  This in the first documentation of natural exposure of cats to N. caninum.


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