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ARS Home Northeast Area Beltsville, Maryland (BHNRC) Beltsville Human Nutrition Research Center Food Composition and Methods Development Laboratory Research Publications at this Location





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Title:   Author  item
                                            Pawlosky, Robertitem
                                            Novotny, Janetitem
                                            Britz, Stevenitem
                                            Clevidence, Beverly
  Submitted to: Present Knowledge in Nutrition  Book Chapter
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 12/1/2000
                                    Publication  Date: 8/1/2001
                                    Citation: Pawlosky, R.J., Novotny Dura, J., Britz, S.J., Clevidence, B.A. 2001. Methods for determining the metabolism of alpha-linolenic acid in humans   from intrinsically-labled food. Present Knowledge in Nutrition.  Book Chapter.56:75-77.Interpretive Summary:   An analysis was made of the metabolism of alpha-linolenic   acid (18:3n-3) to the long-chain polyunsaturated fatty acids   (PUFAs), 20:5n-3, 22:5n-3, and 22:6n-3 that had been labeled   with carbon-13 from kale that had been grown in a carbon-13   atmosphere and fed to a human subject. Gas-chromatography    and mass spectrometry were used to measure the fatty acids.   The time-course profiles and rate constants were determined   using a software program (WinSAAM, Windows Simulation and   Modeling program, National Cancer Institute, NIH). This   model was useful in providing an understanding on how long-   chain polyunsaturated fatty acids are made in humans   beginning with linolenic acid that was derived from a   labeled-food.  The results were compared to another human    study that was carried out using a chemically synthesized   form of the compound.
                                Technical Abstract:   A physiologic multi-compartmental model for the metabolism   of alpha-linolenic acid (13C-18:3n-3) to the long-chain   polyunsaturated fatty acids (PUFAs), 13C-20:5n-3, 13C-22:5n-   3, and 13C-22:6n-3 was constructed from data obtained from a   human subject who had consumed 0.513 g of 13C-18:3n-3 from a    labeled-food (kale). The concentrations of the labeled-fatty   acids were determined by gas-chromatography-mass   spectrometry in the negative ion mode and GC-flame   ionization detection was used to determine the fatty acid   total amounts of the fatty acids. The kinetic profiles and   fractional transfer rate constants were determined by using   the WinSAAM (Windows Simulation and Modeling program) to   construct the appropriate time-curves, which were fitted to    the data. This compartmental model was useful in providing a   framework for quantifying long-chain PUFA production in the   metabolism of linolenic acid that was derived from a   labeled-food and the results were comparable to a study that   was carried out using a chemically synthesized form of   labeled-linoleate.


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