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Title:   Author  item
                                            Goodwin, Stephen

                                                         - *Steve*item
                                            URMEEV, FLEORA - PURDUE UNIVERSITY
  Submitted to: Phytopathology
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 6/1/1999
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: Differential display was used to compare gene expression in resistant and susceptible wheat lines following inoculation with the septoria pathogen, Mycosphaerella graminicola.  Treatments were Yecora Rojo (susceptible) and Tadinia (resistant) uninoculated, and the same two cultivars inoculated with M. graminicola.  Tadinia possesses the Stb4 gene for resistance.  Leaf ftissue was collected at 0, 3, 6, 12, 24, 48 and 96 hours after inoculation with a final sampling after 4 weeks.  Total RNA was extracted and subjected to differential display using four specific and eight random primers. Following electrophoresis on denaturing sequencing gels and autoradiography, differentially expressed genes were excised, cloned and sequenced.  Most sequences had no matches in GenBank.  However, three clones had high similarity to a pathogenesis-related protein from Phaseolus vulgaris.  Most genes were expressed in both the susceptible and the  resistant host.  Experiments are underway to confirm the timing of differential expression using both traditional Northern and reverse-Northern analyses.


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