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ARS Home Midwest Area West Lafayette, Indiana Crop Production and Pest Control Research Research Publications at this Location





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Title:   Author  item
                                            Adhikari, Tikaitem
                                            Goodwin, Stephen

                                                         - *Steve*item
                                            DUBCOVSKY, JORGE - UNIV. OF CALIF, DAVISitem
                                            GIECO, JORGE - UNIV. OF CALIF, DAVIS
  Submitted to: Plant Animal and Microbe Genomes Conference
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 1/14/2002
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: Septoria tritici leaf blotch, caused by the ascomycete fungus Mycosphaerella graminicola (anamporph Septoria tritici), is one of the most important diseases of wheat worldwide.  Deployment of resistant varieties is the major approach to controlling the disease, yet only one resistance gene has been mapped in wheat so far.  A set of 70 F10  recombinant-inbred lines (RILs) derived from a cross between the highly resistant cv. Tadinia (which has the Stb4 resistance gene) and the susceptible parent (UC554 x Yecora Rojo) was evaluated for disease reaction and molecular mapping.  The segregation of resistant:susceptible lines following inoculation with an Indiana isolate of M. graminicola conformed to the 1:1 ratio expected for a single major gene.  A 1:1 segregation also was observed in a field test in California.  Bulked segregant analysis identified a number of  amplified fragment length polymorphism (AFLP) markers that were polymorphic between the resistant and susceptible parents and bulks. One AFLP marker amplified with primer pair Eco RI-ACT/Mse I-CAA was 10.6 cM from the resistance gene, and two additional markers flanked the gene at a distance of approximately 31 cM on each side.  Searches for other potentially linked AFLP and microsatellite markers and conversion of the most closely linked AFLP into a sequence characterized amplified region marker are in progress.


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