U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Food and Feed Safety Research: College Station, TX
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Plains Area College Station, Texas Southern Plains Agricultural Research Center Food and Feed Safety Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Poole, Toniitem
                                            Genovese, Kenneth

                                                         - *Ken*item
                                            Callaway, Todditem
                                            Bischoff, Kennethitem
                                            Nisbet, David
  Submitted to: Society of Microbial Ecology and Disease (SOMED) Proceedings
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 10/3/2001
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: Experiments were conducted to investigate the bactericidal activity against a vancomycin resistant enterococci (VRE) 7000221, and other enterococci, in an anaerobic continuous-flow fermentation culture of chicken gastrointestinal microorganisms (CCF). When CCF was challenged with 10**7 CFU/ml VRE, the VRE population decreased to an undetectable level after 48  hours and could not be rescued by addition of vancomycin at 4ug/ml. In vitro experiments with vancomycin resistant E. faecium A256, tylosin and virginiamycin resistant E. faecium 3167 and vancomycin resistant E. faecalis 7000802 demonstrated clearance from the CCF in approximately 4 days. Prophylactic treatment of day-old broiler chicks with CCF protected against VRE cecal colonization after VRE challenge as compared to non CCF treated controls. In four replicate experiments, VRE colonization occurred in less than 5% of chicks that were inoculated with CCF prior to challenge  with VRE. An additional study was designed to determine the effect of subtherapeutic concentrations of vancomycin on the ability of CCF to clear VRE 7000221. Three experimental groups of CCF were inoculated with 10**7 CFU/ml VRE. Two of these groups were treated with subtherapeutic concentrations of vancomycin, 0.1ug/ml or 0.01ug/ml, respectively, while the third group was not treated with vancomycin. Vancomycin treatment increased the length of time VRE remained in both treated groups from 5 days to 9 days. The results of this study suggest that subtherapeutic doses of 0.1ug/ml or 0.01ug/ml vancomycin were not sufficient to provide a selective advantage to the VRE in the microbial microflora typically inhabiting the chicken ceca.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov