U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Cereal Crops Improvement Research: Fargo, ND
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Plains Area Fargo, North Dakota Edward T. Schafer Agricultural Research Center Cereal Crops Improvement Research Research





- Friesen, Timothy
- 2023
- 2022
- Publications
- Plant Diseases (303)

Research Project: Host-Pathogen Interactions Affecting Wheat and Barley **Location:
                            Cereal Crops Improvement Research** 2022 Annual Report **Objectives**
                                

Objective 1. Functionally characterize the pathogen components of the Parastagonospora nodorum-wheat interaction. 
Sub-objective 1.A: Functionally characterize the role of SnTox5 in necrosis induction and colonization of the wheat leaf.
Sub-objective 1.B: Characterize the role of SnTox267 in virulence using laser confocal microscopy.

Objective 2. Characterize the infection strategies of Pyrenophora teres, the net blotch pathogen of barley. 

Objective 3. Identify, validate, and functionally characterize the Pyrenophora teres genes/proteins important in virulence on barley. **Approach**
                                

Fungal diseases pose an economic threat to plant crops throughout the world resulting in billions of dollars in losses annually. A significant amount of work has been done to understand biotrophic host-pathogen interactions. However, less progress has been made to understand the relationship between plants and their necrotrophic pathogens. Here we focus on understanding how the necrotrophic pathogens Pyrenophora teres, causal agent of net blotch of barley, and Parastagonospora nodorum, causal agent of septoria nodorum blotch of wheat, manipulate the host defense to allow pathogen colonization that results in disease. In previous work, we functionally validated several genes/proteins in P. nodorum that contributed to the pathogen’s infection strategy. Here, we will functionally characterize these effectors using modern tools. CRISPR-Cas9-based gene editing will be used to characterize regions of the proteins involved in virulence function, laser confocal microscopy will be used to visualize the role and mode of action of these effector proteins in planta, and comparative transcriptome sequencing of in planta effector infiltrations and inoculations will be used to characterize the host response to these effectors.  To characterize the P. teres f. teres-barley interaction, we will identify and validate candidate effector genes conferring virulence on susceptible barley. Candidate genes will be identified and validated using both P. teres biparental and globally collected natural populations that differ in virulence. QTL analysis and genome wide association study (GWAS) analysis will be used to locate candidate genomic regions harboring effector genes. Once effector genes have been validated using CRISPR-based gene disruption, strains with and without the effector genes will be used in inoculation experiments to characterize the mode of action of each effector, ultimately resulting in the understanding of the infection strategy of the pathogen. This work will provide critical knowledge to breeders and other researchers targeting the control of these important diseases. **Progress Report**
                                

See Annual report for research project 3060-22000-050-00D. **Accomplishments**


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov