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ARS Home Northeast Area Beltsville, Maryland (BHNRC) Beltsville Human Nutrition Research Center Food Components and Health Laboratory Research Publications at this Location





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Title:   Author  item
                                            Novotny, Janetitem
                                            Britz, Stevenitem
                                            Pawlosky, Robertitem
                                            Harrison, Dawnitem
                                            Kelm, Markitem
                                            Flanagan, Vincentitem
                                            BROWN, ELLEN - JOHNS HOPKINSitem
                                            Beecher, Garyitem
                                            Clevidence, Beverly
  Submitted to: Experimental Biology
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 4/15/2001
                                    Publication  Date: 4/15/2001
                                    Citation: Novotny Dura, J., Britz, S.J., Pawlosky, R.J., Harrison, D.J., Kelm, M.A., Flanagan, V.P., Brown, E., Beecher, G.R., Clevidence, B.A. 2001. Absorption of beta-carotene from carbon-13 labeled kale. Experimental Biology.Interpretive Summary:
                                Technical Abstract: Bioavailability of nutrients from fruits and vegetables is key to determining how well they provide health benefits. The objective of this study was to quantitate the absorption of beta-carotene from a green, leafy vegetable.  Kale was grown in an environmental chamber to be entirely labeled with carbon-13.  The growth chamber was sealed, and the  only source of carbon available to kale plants during the growth cycle was carbon-13.  Nutrients were found to be labeled at >97%.  Upon harvest, the labeled kale was fed to a healthy male volunteer as a single 400 gram dose with 30 grams of oil.  Serial blood samples were collected several times on the dose day, and several times per week for 6 weeks following the dose.  Plasma was analyzed for labeled beta-carotene and retinol by LC-MS and GC-MS, respectively. Compartmental modeling was used to analyze the plasma data.  The plasma showed a significant rise in C13-beta-carotene  and C13-retinol following ingestion of the labeled kale, representing substantial absorption of the beta-carotene from the plant matrix.  The labeled beta-carotene was detectable for 6 weeks following the dose, and the labeled retinol was detectable for over 3 weeks following the dose. In conclusion, labeling of green, leafy vegetables with carbon-13 is a feasible way to study bioavailabiltiy of nutrients from plant foods.


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