U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Emerging Pests and Pathogens Research: Ithaca, NY
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Research Units
                        Robert W. Holley Center for Agriculture & Health
                                    Plant, Soil and Nutrition ResearchSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Northeast Area Ithaca, New York Robert W. Holley Center for Agriculture & Health Emerging Pests and Pathogens Research Research





- Filiatrault, Melanie
- Stodghill, Paul
- Swingle, Bryan
- 2023
- 2022
- Publications
- Plant Diseases (303)

Research Project: **Location:
                            Emerging Pests and Pathogens Research** Project Number: 8062-21000-048-000-D
                        
                        Project Type: In-House Appropriated Start Date: Feb 26, 2022
                        
                        End Date: Feb 25, 2027 **Objective:**
                        
                        Objective 1:  Identify genomic resources for development of diagnostics and detection tools for emerging and re-emerging bacterial plant pathogens.
   Sub-objective 1.A: Perform comparative genomics of bacterial pathogens.
   Sub-objective 1.B: Investigate diversity of soft rot Pectobacteriaceae (SRP).

Objective 2: Characterize biology and virulence factors of bacterial plant pathogens and identify their targets in host plants. 
   Sub-objective. 2.A:  Discover and characterize genes that contribute to disease and/or host adaptation of bacterial soft rot pathogens.
   Sub-objective 2.B:  Discover and characterize genes involved with interactions between bacterial species.
   Sub-objective 2.C: Determine the mechanistic basis of socially affected behaviors in bacteria.
   Sub-objective 2.D: Determine the contribution of AlgU to virulence and factors affecting AlgU activity.
   Sub-objective 2.E: Characterize the signaling pathways that impact expression of HiVir gene cluster in Pantoea.

Objective 3: Investigate sustainable strategies for control of bacterial plant diseases.
   Sub-objective. 3.A: Investigate the role of antimicrobials in tolerance to bacterial soft rot pathogens.
   Sub-objective 3.B: Identify genetic markers of soft rot disease tolerance in US Potato Genebank germplasm. **Approach:**
                        
                        Soft rot bacteria, such as Dickeya, Pectobacterium, and Pantoae are among the most important pathogens of vegetables, fruits, and ornamentals. Bacterial diseases of potato and onion alone cause more than $60M in losses annually in the U.S. Despite the extensive amount of research available on bacterial plant pathogens, there is a lack of understanding about how bacterial plant pathogens enter and move within crop production systems and to what degree these diseases are caused by endemic populations. Furthermore, some bacterial species are endemic and, in some environments, a single bacterial species can be represented by a number of different strains, some of which are pathogens, and some of which are non-pathogens with beneficial biocontrol activities. Therefore, determining which bacteria are responsible for disease and furthermore how certain bacterial strains become pathogenic is an area of research that warrants further study. To address this, we will use genome sequencing methods to characterize populations bacteria present in diseased crops. The patterns that emerge at the intersection of pathogen diversity and geographical location will provide key insights on disease emergence as well as identify diagnostic markers able to distinguish pathogens from non-pathogens. For some plant diseases, such as bacterial soft rot (potato: Dickeya spp. and Pectobacterium spp.; onion: Pantoea spp.) many bacterial species may be involved, with the pathogens being members of a broader community of plant-associated microbes. Little is known about the relationships and interactions of plant pathogens with the host, the microbial community, and the environment and the impact on disease outcome. We will investigate bacterial communication mechanisms involved in pathogen fitness and formation of complex communities in plants to identify factors critical for disease. For many bacterial soft rot diseases there are no effective management options. For example, there are no commercially available potato or onion cultivars with soft rot resistance, thus management options for these pathogens are very limited.  Additionally, there is little known about the specific mechanisms involved in host tolerance or susceptibility. To address this, we will first identify and characterize factors that bacteria use to cause disease and then use that information to guide discovery of bacterial control strategies.  Second, we will identify and characterize sources of natural resistance in wild crop relatives to provide information for breeders as well as a source germplasm for breeding resistant varieties. All together this research will lead to improved fundamental understanding of bacterial soft rot disease dynamics and reveal vulnerabilities that can be exploited for control of bacterial plant diseases, helping us work towards the goal of sustainable plant disease management.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov