U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 close



Research
                Research Home
                            National Programs
                            Research Projects
                            Scientific Manuscripts
                            International Engagement
                            Scientific Software/Models
                            Databases and Datasets
                            Office of Scientific Quality Review
            Media
                News & Features
                            Multimedia
                            Briefing Room
                            Down on the Farm
                            Press Room
                            Science in Your Shopping Cart
                            Scientific Discoveries
                            Tellus
                            Under the Microscope
            About ARS
                About ARS Home
                            Staff Directory
                            Labs and Research Centers (Map)
                            Headquarter Offices
                            Organizational Chart
                            Employee Services (REE Employees Only)
                            Office of Outreach, Diversity, and Equal Opportunity
            Work With Us
                Work With Us Home
                            ARS Vacancies at USAJOBS
                            Careers at ARS Information
                            Scientific Collaborations
                            Extramural Agreements
                            1890 Faculty Research Sabbatical ProgramSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Higgins, Jamesitem
                                            Trout, Jamesitem
                                            Fayer, Ronalditem
                                            Shelton, Danielitem
                                            Jenkins, Mark
  Submitted to: Water Research
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 5/1/2003
                                    Publication  Date: 9/1/2003
                                    Citation: Higgins, J.A., Trout, J.M., Fayer, R., Shelton, D.R., Jenkins, M.C. 2003. Recovery and detection of cryptosporidium parvum oocysts from water samples using continuous flow centrifugation. Water Research. (37):3551-3560.Interpretive Summary: Cryptosporidium parvum oocysts were recovered from 10L volumes of water, both source and finished, using continuous flow centrifugation and immunomagnetic separation. To determine recovery, bioassay and nested PCR were used for viability. Fluorescence in situ hybridization probes were used to detect the oocysts. The best oocyst recoveries occurred when using  source water. These methods appear to be both more rapid and economical when compared to traditional methods of oocyst recovery from water samples.
                                Technical Abstract: Continuous flow centrifugation (CFC) was used in conjunction with immunomagnetic separation (IMS) to recover oocysts of Cryptosporidium parvum from 10 L volumes of source and finished water samples. Using a spiking dose of 1 x 105 oocysts, 25% - 106% of spiked oocysts were recovered, with the best recoveries  from source water samples. Recovered oocysts were viable, as determined by bioassay using HCT-8 cells and nested PCR, and were detectable using fluorescence in situ hybridization (FISH) probes directed against the 18S rRNA gene. Using a spiking dose of 1 x 102 oocysts in 10 L of source water, oocysts were recovered in 9 of 10 trials as determined by IFA microscopy, with a mean oocyst recovery of 4.4%. Another 9 of 10 trials were positive by nested PCR for the C. parvum Cp41 and TRAP C-1 genes. No oocysts were detected in unspiked  controls using IFA microscopy or nested PCR. CFC and IMS may provide a more rapid and economical alternative to traditional, capsule filtration-based methods for isolation of C. parvum oocysts from water samples.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov