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Title:   Author  item
                                            Zhu, Jamesitem
                                            Lillehoj, Hyun
  Submitted to: BARC Poster Day
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 12/1/1999
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: Two commercial broiler lines were used in an experiment to map quantitative trait loci (QTL) affecting disease resistance, growth, and reproduction traits.  Chickens from these lines were genotyped with 27 microsatellite markers to estimate heterozygosity and polymorphism information contest (PIC).  Heterozygosity estimated from allelic frequencies was 0.53 and 0.51 1for the two lines; however, heterozygosity calculated from actual counts o heterozygous loci  (0.37 and 0.35, respectively) were much lower than the estimated heterozygosity.  The PIC was less than 0.5 in both lines, and average allele number per marker locus was about 3.5.  Twenty-five males produced from a cross between these two lines were screened with the DNA marker to select birds with high heterozygosity at marker loci.  Mating simulation showed that non-informative matings can be reduced by about 5% if 12 pairs of the males and females with the highest heterozygosity at  marker loci were selected.  However, the percentage of non-informative matings was not reduced further if eight pairs were selected.  This experiment demonstrated that the heterozygosity and PIC in commercial broiler lines were low and selection on the birds with high heterozygosity at marker loci can increase informative content in QTL mapping, thus increasing detection power.


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