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Title:   Author  item
                                            Alexander, Nancyitem
                                            Ziegenhorn, Suzanneitem
                                            MUEHLBAUER, G - U OF MN, ST PAUL, MNitem
                                            McCormick, Susanitem
                                            KURTZ, B - BRADLEY UNIV, PEORIA, IL
  Submitted to: National Fusarium Head Blight Forum
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 12/7/1999
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: Wheat head blight is a plant disease that is caused by the fungus Fusarium graminearum.  This fungus produces a trichothecene toxin called deoxynivalenol (DON) that acts as a protein synthesis inhibitor and is thought to be correlated with the severity of the disease.  It is reasoned that if plants could detoxify DON, then they  may exhibit increased resistance to fungal invasion.  The wheat cultivar Frontana exhibits limited resistance to infection by Fusarium graminearum, and it is possible that this resistance is due to the expression of toxin resistant genes.  Several cDNA libraries were made from both infected and non-infected wheat heads at differing time periods post-infection, as well as tissue cultures grown with or without DON for varying time periods.  For screening, these cDNA libraries were put into a yeast vector and transformed  into a yeast strain that is sensitive to the trichothecene toxins. Selection for resistant colonies was then done on toxin media.  The resulting inserts were sequenced and compared by BLAST for homology with other sequences in GenBank.  Only the fungal gene TRI101 was isolated from the cDNA libraries made from Fusarium-infected wheat heads.  This gene codes for an acetyltransferase which has been shown to detoxify a number of trichothecene toxins, including DON. Analyses are continuing on the remaining libraries.


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