U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Livestock Bio-Systems: Clay Center, NE
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    USMARC Home
                    USMARC Research Units
                        Genetics and Animal Breeding
                                    Meat Safety & Meat Quality
                                    Nutrition, Growth and Physiology
                                    Animal Health GenomicsSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Plains Area Clay Center, Nebraska U.S. Meat Animal Research Center Livestock Bio-Systems Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications
- IACUC Summaries

Title:   Author  item
                                            Vallet, Jeffitem
                                            Fahrenkrug, Scott
  Submitted to: Biology of Reproduction Abstracts
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 4/15/2000
                                    Publication  Date: 12/20/2000
                                    Citation: Vallet, J.L., Fahrenkrug, S.C. 2000. Structure of the gene for uteroferrin [abstract]. Biology of Reproduction. 62(Supplement 1):288. (Abstract No. 462)Interpretive Summary:
                                Technical Abstract: To confirm the structure of the UF gene, a bacterial artificial chromosome (BAC) genomic library was screened using a uteroferrin specific cDNA probe. UF BAC clone DNA was digested with EcoRI resulting in a 14.5 kb fragment containing the gene for UF, which was subcloned into PBS II SK. Approximately 8 kb of DNA,  including the entire gene for UF, was sequenced. The gene for UF spanned 2.5 kb and is comprised of 5 exons, a structure similar to the related tartrate resistant acid phosphatase genes of humans and rats, and different from the previously published UF gene. Comparison of the sequence of the region upstream of the UF gene transcription start site with a previously reported sequence (Gonzalez et al., 1994; DNA Cell Biol. 13: 365) revealed substantial differences. PCR analysis of genomic DNA was used to  determine whether multiple UF genes were present in the pig genome.  Primers from within the gene only amplified products consistent with two newly discovered introns. Primers specific to the 5' region of the current UF gene sequence gave the appropriate product, while primers specific to the previously reported sequence did not. Genomic southern blotting with BamHI, StuI, MscI, SacI, DraI, BamHI + StuI, and BamHI + KpnI using a cDNA (bases 328-567, Simmen et al., 1989) as probe revealed single bands consistent with the current gene sequence. These results necessitate a reexamination of the UF promoter region for potential transcription controlling elements.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov