U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Crop Production and Pest Control Research: West Lafayette, IN
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area West Lafayette, Indiana Crop Production and Pest Control Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications
- Small Grains Viral Disease Laboratory
- Corn and Sorghum Fungal Disease Laboratory
- Host Plant Resistance to Insects Laboratory
- Wheat/Hessian fly Interactions Laboratory
- Small Grains Fungal Disease Laboratory
- Soybean Quality Improvement Laboratory
- Uniform Soybean Tests, Northern Region

Title:   Author  item
                                            Goodwin, Stephen

                                                         - *Steve*item
                                            Cavaletto, Jessica
  Submitted to: Septoria International Workshop Proceedings
                                    Publication Type: Proceedings
                                    Publication Acceptance Date: 9/21/1999
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary: Septoria tritici leaf blotch, caused by the fungus Mycosphaerella graminicola, is one of the most serious diseases of wheat worldwide.  A better understanding of the population biology of this fungus could guide the development of improved disease management strategies.  Much of what is known about the population genetics of this organism has come from  analyses using the DNA fingerprint probe pSTL70.  Although this probe has been used for many years, little is known about the nature of the probe or why it gives a DNA fingerprint.  The most likely hypothesis is that it codes for some sort of movable genetic element or transposon.  To test the transposable element hypothesis, several additional clones of DNA that are related to pSTL70 were identified from M. graminicola. Comparison of the DNA sequences of three clones plus that of the original pSTL70 probe revealed that part of the pSTL70 clone codes for a gene that  in Saccharomyces (bread yeast) is involved in sensing and responding to changes in the concentration of solutes outside a cell.  The function of this gene in M. graminicola remains unknown.  The remaining portion of the pSTL70 clone contains a long DNA sequence that is repeated 3.5 times, and a partial coding sequence for an enzyme that causes DNA to move  These are characteristics of transposons which strongly supports the transposable element hypothesis.  This information will be extremely useful to scientists to understand and explain the DNA fingerprint pattern produced by pSTL70.  It may also make it possible to identify specific DNA fingerprint loci, which could be used by plant pathologists for a quick, easy method for analysis of the population genetics of this important plant pathogen.
                                Technical Abstract: Clones hybridizing to the Mycosphaerella graminicola DNA fingerprint probe pSTL70 were identified from subgenomic libraries and sequenced.  Analyses of the DNA sequences of these clones plus the original pSTL70 clone revealed that pSTL70 contains part of the open reading frame for a probable homologue of an osmosensing histidine kinase gene from yeast.  The  remaining portion of the clone contained a partial reverse transcriptase gene sequence and a 29 base pair direct repeat, which could mean that the clone is a transposable element.  Methods for converting transposable elements into improved DNA fingerprinting techniques are discussed.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov