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Title:   Author  item
                                            THORNTON, C - INTEGRATED RES TECHNOLOGYitem
                                            Whipple, Dianaitem
                                            Palmer, Mitchellitem
                                            CAROTHERS, C - INTEGRATED RES TECHNOLOGYitem
                                            MACLELLAN, K - INTEGRATED RES TECHNOLOGYitem
                                            PASSEN, S - INTEGRATED RES TECHNOLOGY
  Submitted to: International Workshop on Tuberculosis in Animals
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 9/15/1999
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: The specimen processing method based on the use of Zephiran was compared with the C18-carboxypropylbetaine (CB-18) method (JCM 36:3558-3566, 1998) in an attempt to develop an improved antemortem swab test for detecting Mycobacterium bovis infections in livestock. An initial in vitro comparison simply diluted swab samples that had  been spiked with bacilli 1:1 with either 2 mM CB-18 or 0.2% Zephiran. Samples were then subjected to centrifugation and analyzed by culture.  In this simple assay, CB-18 processing increased recovery of M. bovis 1315, which was originally isolated from a white-tailed deer, by three- to four-fold when analyzed by quantitative culture. However, after having been processed with CB-18, M. bovis 1315 was unable to grow in BACTEC 12B cultures that had been supplemented with PANTA.  Further analysis of the components of PANTA indicated that  nalidixic acid at 2.5-20 ug/ml in combination with CB-18 at 10-15 ug/ml was capable of killing this isolate.  Modification of the CB-18 processing protocol was required to enable recovery of the M. bovis 1315 isolate.  These modifications included first incubating the specimen in 2 mM CB-18 (final) and then diluting CB-18 to approximately 0.2 mM prior to centrifugation.


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