U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Food and Feed Safety Research: College Station, TX
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Plains Area College Station, Texas Southern Plains Agricultural Research Center Food and Feed Safety Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Anderson, Robinitem
                                            Harvey, Rogeritem
                                            GENOVESE, KENNETH - 62023010item
                                            Stanker, Larryitem
                                            DELOACH, JOHN - MILK SPECIALTIESitem
                                            Nisbet, David
  Submitted to: International Symposium on Epidemiology and Control of Salmonella in Pork
                                    Publication Type: Proceedings
                                    Publication Acceptance Date: 6/18/1999
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: Numerous bacteriological culture methods have been developed for the qualitative recovery of Salmonella from animal sources but none are considered infallible.  Presently, we report observations on the recovery of Salmonella Choleraesuis (SC) from rectal swabs cultured via schemes using pre-enrichment in tetrathionate broth (TET-scheme) or GN-Hajna broth  (GN-scheme); the latter presumed better for recovering host adapted serotypes.  Piglets (n=12) were challenged at 48 hrs of age with 10**7 colony forming units (CFU) of a novobiocin (NO) and nalidixic acid (NA) resistant strain of SC.  This NO-NA resistant mutant exhibited no apparent cultural differences from the wildtype parent strain when grown as pure cultures in or on a variety of different media, including those listed below.  For the next 21 days post challenge, two rectal swabs were collected daily from each piglet and each was pre-enriched in  tetrathionate or GN-Hajna broth and then further enriched in Rappaport- Vassiladias broth.  Selective differentiation was accomplished on Brilliant Green agar containing 25 micrograms NO/ml (BGANO) and on BGA supplemented with both 25 micrograms NO/ml and 20 micrograms NA/ml (BGANOV/NAL).  Each incubation step was carried out at 37oC for 24 hr. Our marked SC was readily recovered when plated on BGANOV/NAL, although more so (P < 0.10) from swabs cultured via the GN-scheme rather than the TET-scheme (75 and 62%, respectively, of 252 swabs cultured).


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov