U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Food and Feed Safety Research: College Station, TX
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Plains Area College Station, Texas Southern Plains Agricultural Research Center Food and Feed Safety Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications

Title:   Author  item
                                            Poole, Toniitem
                                            Genovese, Kenneth

                                                         - *Ken*item
                                            Anderson, Timothyitem
                                            Bischoff, Kennethitem
                                            Callaway, Todditem
                                            Nisbet, David
  Submitted to: Applied and Environmental Microbiology
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 9/20/2001
                                    Publication  Date: 12/31/2001
                                    Citation: Poole, T.L., Genovese, K.J., Anderson, T.J., Bischoff, K.M., Callaway, T.R., Nisbet, D.J. 2001. Inhibition of a vancomycin-resistant enterococcus by an anaerobic continuous-flow culture of chicken microflora. Microbial Ecology in Health and Disease. 13:246-253.Interpretive Summary: Microorganisms (particularly bacteria) that cause diseases in livestock and poultry are among the most serious problems our farmers face in keeping their animals healthy.  Drugs that kill these microorganisms are called antibiotics and they are very important in animal production both in this country and throughout the world.  One of the problems with  antibiotic use is that, over time, certain disease-causing microorganisms adapt to the antibiotics such that it takes more and more of the drug to work effectively.  This phenomenon is known as antibiotic resistance, and in some cases microorganisms can become so resistant that they can no longer be controlled effectively.  Our research is focused on ways to circumvent the antibiotic resistance problem.  We have discovered that certain harmless bacteria that grow normally in the gut of chickens are able to prevent the growth of, and even kill, a disease-causing bacteria  known as Enterococcus faecium.  This harmful bacteria is known to sometimes occur in meat products that we as consumers eat, and it can cause very serious cases of human food poisoning.  Our work is important because it identifies a possible way to make our food supply safer from harmful microorganisms, it could help in reducing the amount of chemical antibiotics that farmers have to use, and it would certainly contribute to lessening the chance of harmful microorganisms becoming resistant to the antibiotics that are used.
                                Technical Abstract: During experiments to study horizontal transfer of vancomycin resistance among enterococci present in an anaerobic continuous-flow fermentation culture of chicken gastrointestinal microorganisms (CCF), bactericidal activity against ATCC # 700221 vancomycin resistant Enterococcus faecium (VRE) was observed. The experiments presented in this report were  conducted to investigate bactericidal activity against VRE. When CCF was challenged with 10**7 CFU/ml VRE, the VRE population decreased to an undetectable level within 48 hours and could not be rescued by addition of vancomycin at 4ug/ml. In a reciprocal experiment, VRE at 10**8 CFU/ml, was challenged with 10**7 CFU/ml of CCF. CCF displaced VRE by 15 days in all replicate experiments. When CCF and VRE were simultaneously inoculated with 10**7 CFU/ml, VRE was undetectable in 7 days. Prophylactic treatment of day-old broiler chicks with CCF prevented VRE cecal colonization after  VRE challenge as compared to the untreated controls. In four replicate experiments, VRE colonization occurred in less than 5% of chicks that were inoculated with CCF prior to challenge with VRE. A bacteriocin agar overlay assay identified three E. faecalis isolates from CCF that exhibited inhibitory properties toward all E. faecium tested; this included three strains of E. faecium isolated from chickens, four isolated from swine, and five obtained from the ATCC. No inhibition of Enterococcus spp. other than E. faecium was observed. The results of this study suggest that CCF may be an effective inhibitor of vancomycin resistant E. faecium.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov