U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Crop Production and Pest Control Research: West Lafayette, IN
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    CareersSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Midwest Area West Lafayette, Indiana Crop Production and Pest Control Research Research Publications at this Location





- ARS National Programs
- Search for a scientific manuscript
- Search for a research project
- ARS Software
- Programs and Projects
- Collaborations
- Subjects of Investigation
- Publications
- Small Grains Viral Disease Laboratory
- Corn and Sorghum Fungal Disease Laboratory
- Host Plant Resistance to Insects Laboratory
- Wheat/Hessian fly Interactions Laboratory
- Small Grains Fungal Disease Laboratory
- Soybean Quality Improvement Laboratory
- Uniform Soybean Tests, Northern Region

Title:   Author  item
                                            Goodwin, Stephen

                                                         - *Steve*item
                                            URMEEV, FLEORA - PURDUE UNIVERSITY
  Submitted to: Plant and Animal Genome Conference Proceedings
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 1/8/2000
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: Differential display was used to compare gene expression in resistant and susceptible wheat lines following inoculation with the septoria tritici blotch pathogen, Mycosphaerella graminicola, as part of a project to analyze the functional genomics of pest resistance in cereal crops.  Treatments consisted of the susceptible  cultivar Yecora Rojo and the resistant cultivar Tadinia uninoculated, and the same two cultivars inoculated with an Indiana isolate of M. graminicola.  Tadinia possesses Stb4, a single, dominant gene for resistance.  Leaf tissue was collected at 0, 3, 6, 12, 24, 48 and 96 hours after inoculation, with a final sampling after 4 weeks.  Total RNA was extracted and subjected to differential display using three specific and eight random primers. Following electrophoresis on denaturing sequencing gels and  autoradiography, differentially expressed genes were excised from the gels, cloned and sequenced.  Most sequences had no matches in GenBank.  However, three clones had high similarity to a pathogenesis-related protein from Phaseolus vulgaris, one was a protein disulfide isomerase and two were probably cutinase. Surprisingly, most genes were expressed following inoculation in both the susceptible and the resistant host.  Northern analysis showed that the gene for protein disulfide isomerase was expressed in both the resistant and susceptible host at 3 and 6 hours following inoculation, but that expression was much stronger in the resistant than the susceptible host.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov