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Title:   Author  item
                                            Reeves  Iii, Jamesitem
                                            McCarty, Gregoryitem
                                            REEVES, V - CVM, FDA
  Submitted to: Journal of Agricultural and Food Chemistry
                                    Publication Type: Peer Reviewed Journal
                                    Publication Acceptance Date: 12/4/2000
                                    Publication  Date: 2/25/2001
                                    Citation: N/AInterpretive Summary: The interaction of light with various materials (spectroscopy) has come to be widely used to determine the composition of a variety of materials ranging from animal feeds to foods. Infrared light is beyond the range of human sight and is what we commonly perceive as heat.  Soil samples were analyzed  conventionally and by mid-infrared spectroscopy for total carbon, total nitrogen, pH and measures of biological activity.  Ground, but otherwise untreated samples (n = 180), from experimental farm plots (two locations, three replicate plots, under plow and no- till practices, three rates of nitrogen fertilizer and from five depths), were scanned from using a custom made linear sample transport (50 by 2 mm sample area scanned).  Results showed that accurate determination of a number of compositional parameters:  total carbon, total nitrogen, pH and many measures of biological activity could be achieved.  In general, the results achieved using mid-infrared spectra were at least as accurate as those found previously using near-infrared spectra (another type of light), and were sometimes significantly better, i.e., pH.
                                Technical Abstract: Soil samples were analyzed conventionally and by mid-infrared diffuse reflectance spectroscopy for total C, total N, pH and measures of biological activity.  Ground, non-KBr diluted, samples (n = 180) from experimental plots (two locations, three replicate plots, under plow and no-till practices, three rates of  N fertilizer and from five depths), were scanned from 4000 to 400 cm-1 ( 4-cm-1 resolution, 64 co-added scans) on a DigiLab FTS-60 Fourier transform spectrometer using a custom made linear sample transport (50 by 2 mm sample area scanned).  Results using partial least squares regression showed that accurate calibrations can be developed for the determination of a number of compositional parameters: total C, total N, pH and many measures of biological activity.  In general, the results  achieved using mid-infrared spectra were at least as accurate as those found previously using near-infrared spectra, and were sometimes significantly better, i.e., pH.


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