U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Emerging Pests and Pathogens Research: Ithaca, NY
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Research Units
                        Robert W. Holley Center for Agriculture & Health
                                    Plant, Soil and Nutrition ResearchSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Northeast Area Ithaca, New York Robert W. Holley Center for Agriculture & Health Emerging Pests and Pathogens Research Research





- Wang, Xiaohong
- Hansen, Zachariah
- 2023
- 2022
- Publications
- Plant Diseases (303)

Research Project: Biology and Management of Invasive Plant Pathogens Affecting Potato and Soybean **Location:
                            Emerging Pests and Pathogens Research** 2022 Annual Report **Objectives**
                                

Objective 1: Identify and characterize soybean cyst nematode (SCN) populations in newly detected regions in New York. (NP303, C1, PS1B)

Objective 2: Discover and characterize genes and proteins regulating the interactions between potato and its associated pathogens. (NP303, C2, PS2A)
   Sub-Objective 2.A: Characterize AIM-containing effector protein-encoding genes from PCN and their associated host proteins contributing to nematode parasitism or virulence. 
   Sub-Objective 2.B: Investigate a role of autophagy in potato diseases caused by other invasive potato pathogens.

Objective 3: Investigate novel or improved strategies to manage potato cyst nematodes and/or other invasive potato pathogens. (NP303, C3, PS3A)
   Sub-Objective 3.A:  Determine the resistance of breeding potato clones as well as wild potato clones to G. rostochiensis pathotypes.
   Sub-Objective 3.B: Clone the novel R gene present in the broad-spectrum resistant clone Y1-5.
    Sub-Objective 3.C: Determine the resistance of wild potato clones to G. pallida. **Approach**
                                

In general, nematodes and other invasive pathogens of potato and soybean crops cause severe yield loss, and effective control measures are often lacking. In addition, most nematicides are no longer available; thus alternative control strategies for emerging nematode species and pathotypes are critically needed. New plant biotechnologies will likely provide the basis for the development of novel methods of controlling nematode and other invasive pathogens, but the success of these methods will be dependent upon a complete understanding of the fundamental mechanisms of host-pathogen interactions. One approach is to identify and characterize soybean cyst nematode (SCN) populations in newly detected regions in New York. An extensive statewide SCN survey in NY will be condicted and soli samples will be analyzed to determine SCN population densities. A modified HG type test will be used to determine the virulence phenotypes of selected SCN pouplations. The data on SCN population density and virulence diversity will be used to develop recommendations on SCN management in New York. A second approach is to discover and characterize genes and proteins regulating the interactions between potato and its associated pathogens. AIM-containing effector protein-encoding genes from potato cyst nematodes (PCN) that potentially target the host autophagy machinery will be cloned and characterized by multiple technologies to better understand the function of these effectors in nematode parasitism and virulence. The third approach is to investigate novel or improved strategies to manage potato cyst nematodes and/or other invasive potato pathogens. Working with potato breeders we will continue to use bioassays to evaluate breeding clones and wild potato germplasm for nematode resistance. We will conduct nematode phenotyping of the Y1-5 F1 population and use a RenSeq approach coupled with long-read PacBio SMRT sequencing to identify the novel resistance gene(s) present in the wild potato clone Y1-5. Conceptually novel information on the population dynamics of nematode and other invasive pests of potato and soybean crops, and on host–pathogen interactions will aid in the development of new effective biologically-based disease control strategies. Novel genes and germplasm for disease resistance that are identified will accelerate resistance breeding in potatoes and disease resistant cultivars developed through conventional breeding and genetic engineering can be transferred readily to customers. **Progress Report**
                                

Activities during this reporting period have focused on concluding the old project 8062-22000-022-000D and laying the foundation for the new project 8062-22000-023-000D, which just started in February 2022. Since then, we have propagated soybean lines and established protocols for conducting greenhouse bioassay of SCN (soybean cyst nematode) populations. We have designed experiments for identifying host targets of the GrPIMP4 effector and are in the process of generating plant materials needed for functional characterization studies. In the meantime, we have started testing potato breeding clones and wild potato clones for resistance to Ro1 and Ro2 of the golden nematode or to the pale cyst nematode. We have also initiated the phenotyping of Y1-5 offspring for Ro2 resistance. We expect to collect results of these bioassay tests in the winter of 2022 or in the spring of 2023. **Accomplishments**


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov