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ARS Home Midwest Area West Lafayette, Indiana Crop Production and Pest Control Research Research





- Cai, Guohong
- Plant Genetic Resources, Genomics and Genetic Improvement (301)
- Plant Diseases (303)

Research Project: **Location:
                            Crop Production and Pest Control Research** Project Number: 5020-21220-009-007-S
                        
                        Project Type: Non-Assistance Cooperative Agreement Start Date: Sep 1, 2023
                        
                        End Date: Aug 31, 2024 **Objective:**
                        
                        1 Construct a system for gene knock-out by CRISPR in Fusarium virguliforme and Cercospora sojina; 
2 Use the system to knock-out candidate pathogenicity genes and determine their role in disease development. **Approach:**
                        
                        1. Generate mutants of Fusarium virguliforme and Cercospora sojina expressing Cas9 gene; 
2. Generate mono-, double- and triple- mutants of F. virguliforme and Cercospora sojina in which one or more known or suspected pathogenicity genes are knocked-out, and 
3. Test the pathogenicity of mutants on soybeans, by using the mutant in which a gene believed to play a role in pathogen infection of its host is mutated to infect the plant host, thus validate or invalidate the hypothesis..


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