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Title:   Author  item
                                            DYER, C - MONSANTOitem
                                            Carroll, Jeffery

                                                         - *Jeff Carroll*item
                                            TOUCHETTE, K - UNIVERSITY OF MISSOURIitem
                                            ALLEE, G - UNIVERSITY OF MISSOURIitem
                                            Matteri, Robert
  Submitted to: Journal of Animal Science Supplement
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 7/28/2000
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: The melanocortin type 4 receptor (MCR4) is a hypothalamic receptor signalling for appetite suppression when bound by its ligand, alpha-melanocyte stimulating hormone (alpha-MSH), a product of the pro-opiomelanocortin (POMC) transcript.  The agouti-related protein (AGRP) is another hypothalamic hormone which acts as an antagonist, binding to  MCR4 and thus blocking the appetite-suppressive effects of alpha-MSH. We have recently presented preliminary data on the regulation of AGRP expression in newly weaned pigs; however, concurrent data on the expressions of POMC and MCR4 are required to interpret the physiological significance of altered AGRP expression.  A 245 bp cDNA was generated from porcine hypothalamic RNA by reverse-transcription-polymerase chain reaction (RT-PCR).  The porcine MCR4 sequence was found to be 93% homologous to the corresponding human sequence, and was used to generate a cRNA probe for the efollowing experiment.  Fourteen-day-old nursing pigs were either crossfostered to another sow or weaned onto starter diets containing 0 or 7% spray-dried plasma (n = 8/group).  Piglets were further allocated by size into small (3.5-4.3 kg) and large (4.6-5.7 kg) groups.  Piglets were sacrificed 4 d later for tissue collection.  Hypothalamic MCR4 and POMC mRNA levels (relative to 28S rRNA) were evaluated by slot-blot hybridization.  Hypothalamic POMC mRNA levels did not differ between weaning strategies or size (P>.1).  MCR4 gene expression was higher in large pigs compared to small pigs (.482+/.036 vs .331+/.023, P=.0025), but expression did not differ among weaning groups (P>.3).  These data represent the first characterizations of MCR4 gene expression in a livestock species.


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