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ARS Home Midwest Area Ames, Iowa National Animal Disease Center Infectious Bacterial Diseases Research Research Publications at this Location





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Title:   Author  item
                                            WATERS, W - ISU, VMRI, AMES, IAitem
                                            WANNEMUEHLER, M - ISU, VMRI, AMES, IA.item
                                            Pesch, Bruceitem
                                            Olsen, Stevenitem
                                            Whipple, Dianaitem
                                            Palmer, Mitchell
  Submitted to: International Workshop on Tuberculosis in Animals
                                    Publication Type: Abstract Only
                                    Publication Acceptance Date: 9/15/1999
                                    Publication  Date: N/A
                                    Citation: N/AInterpretive Summary:
                                Technical Abstract: Whitetail deer are significant wildlife reservoirs of Mycobacterium bovis infection of cattle. Recall CD4+ and CD8+ T cell responses are elicited by M. bovis infection of cattle with both CD4+ and CD8+ cells implicated in protective immunity. Few studies, however, have examined lymphocyte subset responses to experimental M. bovis infection of deer. In this study, a  flow cytometric proliferation assay was used to examine the relative contribution of individual peripheral blood mononuclear cell (PBMC) subsets to the recall response to M. bovis infection of deer. Naive deer were challenged with M. bovis by cohabitation with known infected deer. These deer developed significant in vivo (delayed type hypersensitivity) and in vitro (proliferative responses) to M. bovis purified protein derivative (PPD). Selected deer were necropsied and typical tuberculous lesions containing M. bovis were detected within lungs and lung-associated  lymph nodes of challenged deer. The predominant subset of lymphocytes proliferating in response to in vitro stimulation with PPD were CD4+ cells. Proliferative responses were also detected from CD8+,  ë TCR+, and IgM+ cells with the magnitude of this response correlating with the percentage of each subset within the PBMC culture. Additional studies utilizing monoclonal antibodies to block subset proliferation or antigen presentation via MHC I, MHC II, and/or CD1 are underway to further characterize this response.


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