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ARS Home Pacific West Area Davis, California Western Human Nutrition Research Center Obesity and Metabolism Research Research





- Bennett, Brian
- Human Nutrition (107)

Research Project: **Location:
                            Obesity and Metabolism Research** Project Number: 2032-51530-025-011-T
                        
                        Project Type: Trust Fund Cooperative Agreement Start Date: Aug 1, 2018
                        
                        End Date: Jul 31, 2019 **Objective:**
                        
                        Consumption of a diet containing beef at a level consistent with the Dietary Guidelines recommendation for protein foods does not increase circulating plasma TMAO levels, a novel cardiovascular risk factor. These studies are motivated by our preliminary data below. Secondary measures will include classic measures of cardiometabolic disease (e.g. plasma lipids) and measures of endothelial function (e.g. endopat). Results from this study will shed light on whether consumption of a diet high in L-carnitine influences cardio-metabolic disease. **Approach:**
                        
                        Objective 1: Cross-sectional Phenotyping study- Dr. Bennett is Co-PI of the Western Human Nutrition Research Center Nutritional (WHNRC) Phenotyping Study, an observational study in healthy adults examining the effect of diet on intestinal microbiota composition, metabolic syndrome and systemic inflammation (ClinicalTrials.gov identifier NCT02367287). All data obtained is being be uploaded and stored on REDCap, an on-line cloud based clinical database repository hosted by the University of California Davis Medical Center. We are currently collecting stool samples from each study subject and performing 16S rRNA sequencing for microbiota analysis. The sequencing data has already been obtained for a total of 144 subjects. TMAO will be determined by liquid chromatography mass spectrometry (LC-MS).


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