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ARS Home Midwest Area Ames, Iowa National Animal Disease Center Food Safety and Enteric Pathogens Research Research





- Loving, Crystal
- Food Safety, (Animal and Plant Products) (108)

Research Project: **Location:
                            Food Safety and Enteric Pathogens Research** Project Number: 5030-32000-225-021-I
                        
                        Project Type: Interagency Reimbursable Agreement Start Date: Jan 1, 2021
                        
                        End Date: Dec 31, 2024 **Objective:**
                        
                        We plan to develop and evaluate a custom set of location specific bacteriophages, selected through machine learning, and tested in Shiga toxin-producing Escherichia coli (STEC) inoculated cattle. **Approach:**
                        
                        1.	Develop a phage library and develop a plate-based activity assays to characterize the efficacy of various phages against a panel of E. coli O157 strains, including key E. coli O157 clades known to be persistent at a specific non-commercial research feed yard.
2.	Predict and select phages with distinct activity profiles and test these in combination on feedlot E. coli O157 isolates in liquid culture assays looking for those that prevent bacterial outgrowth. Test activity of optimum phage combinations with E. coli O157 attached to bovine epithelial cells/2-D organoids as well as when cultured in bovine rectal mucus.
3.	Test the efficacy of the phage cocktail in calves inoculated with E. coli strains known to persist in a specific non-commercial research feed yard.


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