U.S. flagAn official website of the United States government
                    Here's how you know **Official websites use .gov**
                            
                            A **.gov** website belongs to an official government organization in the United States.
 **Secure .gov websites use HTTPS**
                            
                            A **lock** (                            
                              A locked padlock                                                      
                            
                            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Menu Agricultural Research Service
						
                    U.S. DEPARTMENT OF AGRICULTURE
 Crops Pathology and Genetics Research: Davis, CA
 close



    Research

                    Research Projects
                                    Publications
                                    Collaborations
                                    News
                People

                    Staff Listing
                                    Careers


        


                    Outreach Events
                        UC Davis Picnic Day
                                    Kinders go to College
                                    STEM Girls
                                    Future Scientist Program
                                    Agriculture Future of America
                                    Wallace-Carver Fellowship
                                    Young Scholars Program
                                    Annual Awards Banquet
                    About Us
                        About Us
                                    Our Mission
                                    Principal InvestigatorsSearch small
                    Search
            
            ARS Home
                                About ARS
                                Contact Us




ARS Home Pacific West Area Davis, California Crops Pathology and Genetics Research Research





- Sudarshana, Mysore
- Plant Diseases (303)

Research Project: **Location:
                            Crops Pathology and Genetics Research** Project Number: 2032-22000-017-005-R
                        
                        Project Type: Reimbursable Cooperative Agreement Start Date: Oct 1, 2019
                        
                        End Date: Aug 31, 2024 **Objective:**
                        
                        1)Identify alternative hosts and reservoirs of grapevine red blotch virus (GRBV).
2)Determine presence and biology of potential treehopper vectors in symptomatic vineyards and associated crop and non-crop landscapes.
3)Improve knowledge of GRBV acquisition and transmission by its vector(s).
4) Develop cost-effective GRBV diagnostic tools. **Approach:**
                        
                        Insect host plants will be tested for GRBV presence by PCR or qPCR. For any non-Vitis host that test positive for GRBV, additional tests will be done to confirm virus replication in the host by total nucleic acid extraction, digestion with DNAse, then RT-PCR to confirm the presence of virus specific RNA in the plant.
Conduct in-depth insect vector-related studies with focus on treehoppers (Membracidae) associated with vineyards. Vineyards where virus spread is suspected representing both CA and OR will be visually surveyed for treehoppers and other phloem-feeding Hemiptera using sweep net, beat sheet, sticky trap, or vacuum sampling as appropriate.
Characterize GRBV transmission by determining acquisition and inoculation access periods, latent period, virus retention, and persistence of vector infectivity to increase knowledge of transmission.
Develop a field deployable method that can be used to detect GRBV by testing laboratories and vineyard personnel. Two methods (1) LAMP methods (Loop-amplification technology) and thermostable helicase dependent amplification (tHDA), currently under development in Sudarshana lab, will be compared for simplicity, sensitivity and cost of deployment. We have already designed a pair of primers for tHDA-mediated method for use in combination with EvaGreen, dsDNA binding dye for real-time monitoring of the amplification process. This will be compared with latest pH indicator-based monitoring of DNA amplification during LAMP. Amplified DNA will also be examined using a field deployable portable blue LED lamp.


Return to top


Connect with ARS Sign up


ARS Home
                            USDA.gov
                            Plain Writing
                            Policies & Links
                            Civil Rights Statements
                            FOIA
                            Accessibility Statement
                            Privacy Policy
                            Non-Discrimination Statement
                            Quality of Information
                            USA.gov
                            WhiteHouse.gov