UNITED
STATES

SECURITIES
AND EXCHANGE COMMISSION

Washington,
D.C. 20549










FORM 10-K/A

(Amendment
No. 2)








(Mark One)

| | x | ANNUAL
                                         REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934
| | - | -----------------------------------------------------------------------------------------------------------------------------

For
the fiscal year ended June 30, 2017



| |  ̈ | TRANSITION
                                         REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934
| | - | ---------------------------------------------------------------------------------------------------------------------------------



Commission
File Number 333-146542










AYTU
BIOSCIENCE, INC.

(Exact
Name of Registrant as Specified in Its Charter)










|Delaware                                                                               | 47-0883144                                                     
|-------------------------------------------------------------------------------------- | ---------------------------------------------------------------
|(State
        or other jurisdiction of
        incorporation
        or organization) | (I.R.S.
        Employer
        Identification
        Number)



|373
        Inverness Parkway
        Suite
        206
        Englewood,
        Colorado | 80112     
|------------------------------------------------------------------------------------------- | ----------
|(Address of principal executive
    offices)                                                | (Zip Code)



(720) 437-6580

(Registrant’s
telephone number, including area code)

Securities
registered pursuant to Section 12(b) of the Act: None

Securities
registered pursuant to Section 12(g) of the Act

Common
Stock, par value $.0001 per share










Indicate
by check mark if the Registrant is a well-known seasoned issuer, as defined in Rule 405 of the Securities Act.   Yes   ̈    No  x



Indicate by check mark if the
Registrant is not required to file reports pursuant to Section 13 or Section 15(d) of the Exchange Act.    Yes   ̈    No  x



Indicate by a check mark whether
the Registrant: (1) has filed all reports required to be filed by Section 13 or 15(d) of the Securities Exchange Act
of 1934 during the preceding 12 months (or for such shorter period that the Registrant was required to file such reports)
and (2) has been subject to such filing requirements for the past 90 days.    Yes  x    No   ̈



Indicate by check mark whether
the registrant has submitted electronically and posted on its corporate Web site, if any, every Interactive Data File required
to be submitted and posted pursuant to Rule 405 of Regulation S-T during the preceding 12 months (or for such shorter
period that the registrant was required to submit and post such files).    Yes  x    No   ̈



Indicate by check mark if disclosure
of delinquent filers pursuant to Item 405 of Regulation S-K is not contained herein, and will not be contained, to the
best of the Registrant’s knowledge, in definitive proxy or information statements incorporated by reference in Part III
of this Form 10-K or any amendment to this Form 10-K  ̈



Indicate by check mark whether
the Registrant is a large accelerated filer, an accelerated filer, a non-accelerated filer, or a smaller reporting company. See
definition of “large accelerated filer”, “accelerated filer” and “smaller reporting company”
in Rule 12b-2 of the Exchange Act. (check one):



|Large accelerated filer |  ̈                                                  | Accelerated filer         |  ̈
|----------------------- | -------------------------------------------------- | ------------------------- | -
|                        |                                                    |                           |  
|Non-accelerated filer   |  ̈(Do
    not check if a smaller reporting company) | Smaller reporting company | x
|                        |                                                    | Emerging growth company   |  ̈



If an emerging growth company,
indicate by check mark if the registrant has elected not to use the extended transition period for complying with any new or revised
financial accounting standards provided pursuant to Section 13a) of the Exchange Act.  ̈



Indicate by check mark whether
the Registrant is a shell company (as defined in Rule 12b-2 of the Exchange Act).     Yes   ̈    No  x



The aggregate
market value of common stock held by non-affiliates of the Registrant as of December 31, 2016 was $10.5 million based on
the closing price of $24.00 as of that date.



Indicate the number of shares
outstanding of each of the Registrant’s classes of common stock, as of the latest practicable date:



As of August
15, 2017, there are 4,021,822 shares of common stock outstanding and 2,250 shares of Series A preferred stock outstanding.


















EXPLANATORY NOTE





This Amendment No.
2 to Form 10-K (this “Amendment”) amends Part IV of the Annual Report on Form 10-K for the fiscal year ended June 30,
2017 of Aytu BioScience, Inc., as originally filed with the Securities and Exchange Commission (“SEC”) on August 31,
2017 and amended on October 12, 2017 (the “Original Form 10-K”). This Amendment attaches Exhibit 10.25 which is subject
to a request for confidential treatment. Certain portions of the attached Exhibit 10.25 that were previously redacted are being
disclosed in this Amendment.



Pursuant to Rule 12b-15
under the Securities Exchange Act of 1934, as amended, this Amendment also contains current dated certifications from the Principal
Executive Officer and the Principal Financial Officer.



Except as described
above, no other changes have been made to the Original Form 10-K. This Amendment does not otherwise update information in the
Original Form 10-K to reflect facts or events occurring subsequent to the filing date of the Original Form 10-K. This Amendment
should be read in conjunction with the Original Form 10-K and with any of our filings made with the SEC subsequent to filing of
the Original Form 10-K.















TABLE OF CONTENTS



|              |                                                | PAGE
|------------- | ---------------------------------------------- | ----
|              |                                                |     
|              | PART
    IV                                    |     
|              |                                                |     
|Item 15       | EXHIBITS AND FINANCIAL
    STATEMENT SCHEDULES | 4   
|              |                                                |     
|SIGNATURES    | 7                                             
|Exhibit 10.25 |                                                |     
|Exhibit 31.1  |                                                |     
|Exhibit 31.2  |                                                |     
|Exhibit 32.1  |                                                |     



This Annual
Report on Form 10-K refers to trademarks, such as Aytu, Natesto, ProstaScint, Primsol, MiOXSYS, RedoxSYS, and Fiera which are
protected under applicable intellectual property laws and are our property or the property of our subsidiaries. This Form 10-K
also contains trademarks, service marks, copyrights and trade names of other companies which are the property of their respective
owners. Solely for convenience, our trademarks and tradenames referred to in this Form 10-K may appear without the ®or TM symbols, but such references are not intended to indicate in any way that we will not assert, to the fullest
extent under applicable law, our rights to these trademarks and tradenames.



Unless
otherwise indicated or unless the context otherwise requires, references in this Form 10-K to the “Company,” “Aytu,”
“we,” “us,” or “our” are to Aytu BioScience, Inc.





3









PART IV



Item 
15.	Exhibits and Consolidated Financial Statement Schedules



| | (a)(1) | Financial Statements
| | ------ | --------------------



The following documents
are filed as part of this Form 10-K, as set forth on the Index to Financial Statements found on page F-1.



| | • | Report of Independent Registered
                                         Public Accounting Firm
| | - | ------------------------------------------------------------------------------------------------



| | • | Consolidated Balance Sheets
                                         as of June 30, 2017 and 2016
| | - | -------------------------------------------------------------------------------------------------



| | • | Consolidated Statements
                                         of Operations for the years ended June 30, 2017 and 2016
| | - | -------------------------------------------------------------------------------------------------------------------------



| | • | Consolidated Statements
                                         of Stockholders’ Equity (Deficit) for the years ended June 30, 2017 and 2016
| | - | ---------------------------------------------------------------------------------------------------------------------------------------------



| | • | Consolidated Statements
                                         of Cash Flows for the years ended June 30, 2017 and 2016
| | - | -------------------------------------------------------------------------------------------------------------------------



| | • | Consolidated Notes to the
                                         Financial Statements
| | - | ---------------------------------------------------------------------------------------



| | (a)(2) | Financial Statement
                                         Schedules
| | ------ | ----------------------------------------------------------------------



Not Applicable.



4







| | (a)(3) | Exhibits
| | ------ | --------





|Exhibit No. |  | Description                                                                                                                                                                                                                                                                                                                  |  | Registrant’sForm |  | DateFiled |  | ExhibitNumber |  | FiledHerewith
|----------- |  | ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- |  | ---------------- |  | --------- |  | ------------- |  | -------------
|            |  |                                                                                                                                                                                                                                                                                                                              |  |                  |  |           |  |               |  |              
|3.1         |  | Certificate
    of Incorporation                                                                                                                                                                                                                                                                                             |  | 8-K              |  | 6/09/15   |  | 3.1           |  |              
|            |  |                                                                                                                                                                                                                                                                                                                              |  |                  |  |           |  |               |  |              
|3.2         |  | Certificate
    of Amendment of Certificate of Incorporation effective June 1, 2016                                                                                                                                                                                                                                          |  | 8-K              |  | 6/02/16   |  | 3.1           |  |              
|            |  |                                                                                                                                                                                                                                                                                                                              |  |                  |  |           |  |               |  |              
|3.3         |  | Certificate
    of Amendment of Certificate of Incorporation, effective June 30, 2016                                                                                                                                                                                                                                        |  | 8-K              |  | 7/01/16   |  | 3.1           |  |              
|            |  |                                                                                                                                                                                                                                                                                                                              |  |                  |  |           |  |               |  |              
|3.4         |  | Certificate
    of Designation of Preferences, Rights and Limitations of Series A Convertible Preferred Stock, filed on August 11, 2017.                                                                                                                                                                                     |  | 8-K              |  | 8/16/17   |  | 3.1           |  |              
|            |  |                                                                                                                                                                                                                                                                                                                              |  |                  |  |           |  |               |  |              
|3.5         |  | Certificate
    of Amendment of Certificate of Incorporation, effective August 25, 2017                                                                                                                                                                                                                                      |  | 8-K              |  | 8/29/17   |  | 3.1           |  |              
|            |  |                                                                                                                                                                                                                                                                                                                              |  |                  |  |           |  |               |  |              
|3.6         |  | Bylaws                                                                                                                                                                                                                                                                                                                       |  | 8-K              |  | 6/09/15   |  | 3.2           |  |              
|            |  |                                                                                                                                                                                                                                                                                                                              |  |                  |  |           |  |               |  |              
|4.2         |  | Form
    of Placement Agent Warrant issued in 2015 Convertible Note Financing                                                                                                                                                                                                                                                |  | 8-K              |  | 7/24/15   |  | 4.2           |  |              
|            |  |                                                                                                                                                                                                                                                                                                                              |  |                  |  |           |  |               |  |              
|4.3         |  | Warrant
    Agent Agreement, dated May 6, 2016 by and between Aytu BioScience, Inc. and VStock Transfer, LLC.                                                                                                                                                                                                                |  | 8-K              |  | 5/6/16    |  | 4.1           |  |              
|            |  |                                                                                                                                                                                                                                                                                                                              |  |                  |  |           |  |               |  |              
|4.4         |  | First
    Amendment to May 6, 2016 Warrant Agent Agreement between Aytu BioScience, Inc. and VStock Transfer LLC.                                                                                                                                                                                                            |  | S-1              |  | 9/21/16   |  | 4.5           |  |              
|            |  |                                                                                                                                                                                                                                                                                                                              |  |                  |  |           |  |               |  |              
|4.5         |  | Warrant
    Agent Agreement, dated November 2, 2016 by and between Aytu BioScience, Inc. and VStock Transfer, LLC.                                                                                                                                                                                                           |  | 8-K              |  | 11/2/16   |  | 4.1           |  |              
|            |  |                                                                                                                                                                                                                                                                                                                              |  |                  |  |           |  |               |  |              
|4.6         |  | Form
    of Amended and Restated Underwriters’ Warrant (May 2016 Financing)                                                                                                                                                                                                                                                  |  | 8-K              |  | 3/1/17    |  | 4.1           |  |              
|            |  |                                                                                                                                                                                                                                                                                                                              |  |                  |  |           |  |               |  |              
|4.7         |  | Form
    of Amended and Restated Underwriters’ Warrant (October 2016 Financing)                                                                                                                                                                                                                                              |  | 8-K              |  | 3/1/17    |  | 4.2           |  |              
|            |  |                                                                                                                                                                                                                                                                                                                              |  |                  |  |           |  |               |  |              
|4.8         |  | Form
    of Common Stock Purchase Warrant issued on August 15, 2017.                                                                                                                                                                                                                                                         |  | 8-K              |  | 8/16/17   |  | 4.1           |  |              
|            |  |                                                                                                                                                                                                                                                                                                                              |  |                  |  |           |  |               |  |              
|10.1†       |  | Form
    of Indemnification Agreement, to be entered into between the Registrant and its directors and officers                                                                                                                                                                                                              |  | 8-K              |  | 4/22/15   |  | 10.1          |  |              
|            |  |                                                                                                                                                                                                                                                                                                                              |  |                  |  |           |  |               |  |              
|10.2#       |  | Asset
    Purchase Agreement between the Registrant (as assigned to it by Ampio/Vyrix) and Valeant International (Barbados) SRL, effective
    as of December 2, 2011                                                                                                                                                        |  | 8-K/A            |  | 6/08/15   |  | 10.4          |  |              
|            |  |                                                                                                                                                                                                                                                                                                                              |  |                  |  |           |  |               |  |              
|10.3#       |  | Manufacturing
    and Supply Agreement between the Registrant (as assigned to it by Ampio/Vyrix) and Ethypharm S.A., dated September 10, 2012                                                                                                                                                                                |  | 8-K/A            |  | 6/08/15   |  | 10.5          |  |              
|            |  |                                                                                                                                                                                                                                                                                                                              |  |                  |  |           |  |               |  |              
|10.4        |  | License,
    Development and Commercialization Agreement between the Registrant (as assigned to it by Ampio/Vyrix) and Daewoong Pharmaceuticals
    Co., Ltd., effective as of August 23, 2011 (incorporated by reference to Exhibit 10.1 of Ampio Pharmaceutical’s Form
    8-K/A filed October 5, 2011;File No. 001-25182) |  |                  |  |           |  |               |  |              
|            |  |                                                                                                                                                                                                                                                                                                                              |  |                  |  |           |  |               |  |              
|10.5#       |  | Distribution
    Agreement between the Registrant (as assigned to it by Ampio/Vyrix) and FBM Industria Farmaceutica, Ltda., dated as ofMarch 1, 2012                                                                                                                                                                         |  | 8-K/A            |  | 6/08/15   |  | 10.7          |  |              
|            |  |                                                                                                                                                                                                                                                                                                                              |  |                  |  |           |  |               |  |              
|10.6#       |  | Distribution
    and License Agreement between the Registrant (as assigned to it by Ampio/Vyrix) and Endo Ventures Limited, dated April 9,
    2014                                                                                                                                                                          |  | 8-K/A            |  | 6/08/15   |  | 10.8          |  |              
|            |  |                                                                                                                                                                                                                                                                                                                              |  |                  |  |           |  |               |  |              
|10.7#       |  | Sponsored
    Research Agreement between the Registrant (as assigned to it by Ampio/Luoxis) and Trauma Research LLC, dated September 1,
    2009                                                                                                                                                                             |  | 8-K/A            |  | 6/08/15   |  | 10.9          |  |              
|            |  |                                                                                                                                                                                                                                                                                                                              |  |                  |  |           |  |               |  |              
|10.8#       |  | Addendum
    No. 4 to Sponsored Research Agreement between the Registrant (as assigned to it by Ampio/Luoxis) and Trauma Research LLC,
    dated March 17, 2014                                                                                                                                                              |  | 8-K              |  | 5/27/15   |  | 10.14         |  |              
|            |  |                                                                                                                                                                                                                                                                                                                              |  |                  |  |           |  |               |  |              
|10.9        |  | Promissory
    Note issued by Ampio to the Registrant on April 16, 2015                                                                                                                                                                                                                                                      |  | 8-K              |  | 4/22/15   |  | 10.11         |  |              
|            |  |                                                                                                                                                                                                                                                                                                                              |  |                  |  |           |  |               |  |              
|10.10       |  | Subscription
    Agreement between the Registrant and Ampio, dated April 16, 2015                                                                                                                                                                                                                                            |  | 8-K              |  | 4/22/15   |  | 10.12         |  |              
|            |  |                                                                                                                                                                                                                                                                                                                              |  |                  |  |           |  |               |  |              
|10.11       |  | Voting
    Agreement between the Registrant and Ampio, dated April 21, 2015 (incorporated by reference to Exhibit 10.1 to Ampio’s
    Form 8-K filed April 22, 2015; File No. 001-35182)                                                                                                                                     |  |                  |  |           |  |               |  |              
|            |  |                                                                                                                                                                                                                                                                                                                              |  |                  |  |           |  |               |  |              
|10.12       |  | Asset
    Purchase Agreement between Jazz Pharmaceuticals, Inc. and Rosewind Corporation, dated May 20, 2015                                                                                                                                                                                                                 |  | 8-K              |  | 5/27/15   |  | 10.14         |  |              
|            |  |                                                                                                                                                                                                                                                                                                                              |  |                  |  |           |  |               |  |              
|10.13       |  | Form
    of Note Purchase Agreement for 2015 Convertible Note Financing                                                                                                                                                                                                                                                      |  | 8-K              |  | 7/24/15   |  | 10.1          |  |              





5









|Exhibit No. |  | Description                                                                                                                                                                                                                                                                                                                                                                                         |  | Registrant’sForm |  | DateFiled |  | ExhibitNumber |  | FiledHerewith
|----------- |  | --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- |  | ---------------- |  | --------- |  | ------------- |  | -------------
|            |  |                                                                                                                                                                                                                                                                                                                                                                                                     |  |                  |  |           |  |               |  |              
|10.14       |  | Asset
    Purchase Agreement, dated October 5, 2015, between Aytu BioScience, Inc. and FSC Laboratories, Inc.                                                                                                                                                                                                                                                                                       |  | 8-K              |  | 10/07/15  |  | 10.18         |  |              
|            |  |                                                                                                                                                                                                                                                                                                                                                                                                     |  |                  |  |           |  |               |  |              
|10.15       |  | Master
    Services Agreement between Biovest International, Inc. and Aytu BioScience, Inc., entered into on October 8, 2015, and effective
    October 5, 2015                                                                                                                                                                                                                                     |  | 8-K              |  | 10/13/15  |  | 10.19         |  |              
|            |  |                                                                                                                                                                                                                                                                                                                                                                                                     |  |                  |  |           |  |               |  |              
|10.16       |  | Form
    of Subscription Agreement for January 2016 common stock purchases                                                                                                                                                                                                                                                                                                                          |  | 8-K              |  | 1/20/16   |  | 10.1          |  |              
|            |  |                                                                                                                                                                                                                                                                                                                                                                                                     |  |                  |  |           |  |               |  |              
|10.17       |  | License
    and Supply Agreement between the Registrant and Acerus Pharmaceuticals Corporation, dated April 22, 2016                                                                                                                                                                                                                                                                                |  | 8-K              |  | 4/25/16   |  | 10.1          |  |              
|            |  |                                                                                                                                                                                                                                                                                                                                                                                                     |  |                  |  |           |  |               |  |              
|10.18       |  | Subscription
    Agreement between the Registrant and Acerus Pharmaceuticals Corporation, dated April 22, 2016                                                                                                                                                                                                                                                                                      |  | 8-K              |  | 4/25/16   |  | 10.2          |  |              
|            |  |                                                                                                                                                                                                                                                                                                                                                                                                     |  |                  |  |           |  |               |  |              
|10.19       |  | First
    Amendment, dated May 15, 2016, to Employment Agreement dated September 16, 2015 between Aytu BioScience, Inc. and Jonathan
    McGrael                                                                                                                                                                                                                                                    |  | 8-K              |  | 5/16/16   |  | 10.1          |  |              
|            |  |                                                                                                                                                                                                                                                                                                                                                                                                     |  |                  |  |           |  |               |  |              
|10.20       |  | Purchase
    Agreement, dated July 27, 2016, by and between Aytu BioScience, Inc. and Lincoln Park Capital Fund, LLC.                                                                                                                                                                                                                                                                               |  | 8-K              |  | 7/28/16   |  | 10.1          |  |              
|            |  |                                                                                                                                                                                                                                                                                                                                                                                                     |  |                  |  |           |  |               |  |              
|10.21       |  | Registration
    Rights Agreement dated July 27, 2016, by and between Aytu BioScience, Inc. and Lincoln Park Capital Fund, LLC.                                                                                                                                                                                                                                                                     |  | 8-K              |  | 7/28/16   |  | 10.2          |  |              
|            |  |                                                                                                                                                                                                                                                                                                                                                                                                     |  |                  |  |           |  |               |  |              
|10.22†      |  | Employment
    Agreement, effective as of April 16, 2017, between Aytu BioScience, Inc. and Joshua R. Disbrow.                                                                                                                                                                                                                                                                                      |  | 8-K              |  | 4/18/17   |  | 10.1          |  |              
|            |  |                                                                                                                                                                                                                                                                                                                                                                                                     |  |                  |  |           |  |               |  |              
|10.23†      |  | Employment
    Agreement, effective as of April 16, 2017, between Aytu BioScience, Inc. and Jarrett T. Disbrow.                                                                                                                                                                                                                                                                                     |  | 8-K              |  | 4/18/17   |  | 10.2          |  |              
|            |  |                                                                                                                                                                                                                                                                                                                                                                                                     |  |                  |  |           |  |               |  |              
|10.24       |  | Asset
    Purchase Agreement, dated March 31, 2017, between Allegis Holdings, LLC and Aytu BioScience, Inc.                                                                                                                                                                                                                                                                                         |  | 10-Q             |  | 5/11/17   |  | 10.1          |  |              
|            |  |                                                                                                                                                                                                                                                                                                                                                                                                     |  |                  |  |           |  |               |  |              
|10.25^      |  | Merger Agreement, dated May 3, 2017, between Nuelle, Inc. and Aytu BioScience, Inc.                                                                                                                                                                                                                                                                                                                 |  |                  |  |           |  |               |  | X            
|            |  |                                                                                                                                                                                                                                                                                                                                                                                                     |  |                  |  |           |  |               |  |              
|10.26†      |  | Employment
    Agreement, effective as of June, 2017, between Aytu BioScience, Inc. and Gregory A. Gould.                                                                                                                                                                                                                                                                                           |  | 8-K              |  | 6/19/17   |  | 10.1          |  |              
|            |  |                                                                                                                                                                                                                                                                                                                                                                                                     |  |                  |  |           |  |               |  |              
|10.27†      |  | 2015
    Stock Option and Incentive Plan, as amended on July 26, 2017.                                                                                                                                                                                                                                                                                                                              |  | 8-K              |  | 7/27/17   |  | 10.1          |  |              
|            |  |                                                                                                                                                                                                                                                                                                                                                                                                     |  |                  |  |           |  |               |  |              
|10.28       |  | Securities
    Purchase Agreement, dated August 11, 2017, between Aytu BioScience, Inc. and the investors named therein.                                                                                                                                                                                                                                                                            |  | 8-K              |  | 8/16/17   |  | 10.1          |  |              
|            |  |                                                                                                                                                                                                                                                                                                                                                                                                     |  |                  |  |           |  |               |  |              
|10.29       |  | Registration
    Rights Agreement, dated August 11, 2017, between Aytu BioScience, Inc. and the investors named therein.                                                                                                                                                                                                                                                                            |  | 8-K              |  | 8/16/17   |  | 10.2          |  |              
|            |  |                                                                                                                                                                                                                                                                                                                                                                                                     |  |                  |  |           |  |               |  |              
|23.1        |  | Consent
    of EKS&H LLLP, Independent Registered Public Accounting Firm.                                                                                                                                                                                                                                                                                                                           |  | 10-K             |  | 8/31/17   |  | 23.1          |  |              
|            |  |                                                                                                                                                                                                                                                                                                                                                                                                     |  |                  |  |           |  |               |  |              
|31.1        |  | Certificate
    of the Chief Executive Officer of Aytu BioScience, Inc. pursuant to Section 302 of the Sarbanes-Oxley Act of 2002.                                                                                                                                                                                                                                                                  |  |                  |  |           |  |               |  | X            
|            |  |                                                                                                                                                                                                                                                                                                                                                                                                     |  |                  |  |           |  |               |  |              
|31.2        |  | Certificate
    of the Chief Financial Officer of Aytu BioScience, Inc. pursuant to Section 302 of the Sarbanes-Oxley Act of 2002.                                                                                                                                                                                                                                                                  |  |                  |  |           |  |               |  | X            
|            |  |                                                                                                                                                                                                                                                                                                                                                                                                     |  |                  |  |           |  |               |  |              
|32.1        |  | Certificate
    of the Chief Executive Officer and the Chief Financial Officer of Aytu BioScience, Inc. pursuant to Section 906 of the
    Sarbanes-Oxley Act of 2002.                                                                                                                                                                                                                              |  |                  |  |           |  |               |  | X            
|            |  |                                                                                                                                                                                                                                                                                                                                                                                                     |  |                  |  |           |  |               |  |              
|101         |  | XBRL (extensible Business Reporting Language). The following materials from
    Aytu BioScience, Inc.’s Annual Report on Form 10-K for the year ended June 30, 2017 formatted in XBRL: (i) the Balance
    Sheets, (ii) the Statements of Operations, (iii) the Statements of Stockholders’ Equity (Deficit), (iv) the Statements
    of Cash Flows, and (v) the Notes to the Financial Statements. |  | 10-K             |  | 8/31/17   |  | 101           |  |              












|† |  | Indicates is a management contract
    or compensatory plan or arrangement.                                                                                                                                                                   
|- |  | ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
|# |  | The company has received confidential treatment
    of certain portions of this agreement. These portions have been omitted and filed separately with the Securities and Exchange
    Commission pursuant to a confidential treatment request.
|^ |  | Confidential treatment has been requested with
    respect to certain portions of this exhibit. Omitted portions have been filed separately with the SEC.                                                                                     





6







SIGNATURES



Pursuant to the requirements of
Section 13 or 15(d) of the Securities and Exchange Act of 1934, the Registrant has duly caused this report to be signed on
its behalf by the undersigned, thereunto duly authorized.



|                       |     |                                     
|---------------------- | --- | ------------------------------------
|                       |     | AYTU BIOSCIENCE, INC.               
|                       |     |                                     
|Date: October 19, 2017 | By: | /s/ Joshua R. Disbrow               
|                       |     | Joshua R. Disbrow                   
|                       |     | Chairman and Chief Executive Officer
|                       |     | (Principal Executive Officer)       



Pursuant to the requirements of
the Securities Exchange Act of 1934, this report has been signed below by the following persons on behalf of the Registrant in
the capacities indicated, on October 19, 2017.





|Signature             |  | Title                                       
|--------------------- |  | --------------------------------------------
|                      |  |                                             
|/s/ Joshua R. Disbrow |  | Chairman and Chief Executive Officer        
|Joshua R. Disbrow     |  | (Principal Executive Officer)               
|                      |  |                                             
|/s/ Gregory A. Gould  |  | Chief Financial Officer                     
|Gregory A. Gould      |  | (Principal Financial and Accounting Officer)
|                      |  |                                             
|/s/ Michael Macaluso  |  |                                             
|Michael Macaluso      |  | Director                                    
|                      |  |                                             
|/s/ Carl Dockery      |  |                                             
|Carl Dockery          |  | Director                                    
|                      |  |                                             
|/s/ John Donofrio     |  | Director                                    
|John Donofrio         |  |                                             
|                      |  |                                             
|/s/ Gary Cantrell     |  | Director                                    
|Gary Cantrell         |  |                                             





7