# Patent

## Title
Bacteria mediated gene silencing

## Abstract

Methods are described for the delivery of one or more small interfering RNAs (siRNAs) to a eukaryotic cell using a bacterium or BTP. Methods are also described for using this bacterium to regulate gene expression in eukaryotic cells using RNA interference, and methods for treating viral diseases and disorders. The bacterium or BTP includes one or more siRNAs or one or more DNA molecules encoding one or more siRNAs. Vectors are also described for use with the bacteria of the invention for causing RNA interference in eukaryotic cells.

## Background
RELATED APPLICATIONS
This application is a continuation of U.S. application Ser. No. 12/157,969, which claims priority to, and the benefit of, U.S. Provisional Patent Application No. 60/934,751, filed Jun. 15, 2007 and U.S. Provisional Patent Application No. 61/010,028, filed Jan. 4, 2008. The contents of each of these applications are herein incorporated by reference in their entireties.


SEQUENCE LISTING
This application includes a sequence listing submitted electronically as an ASCII file created on Oct. 30, 2016, named MAR363US_SL.txt, which is 183,172 bytes in size, and is hereby incorporated by reference in its entirety.
BACKGROUND
Gene silencing through RNAi (RNA-interference) by use of short interfering RNA (siRNA) has emerged as a powerful tool for molecular biology and holds the potential to be used for therapeutic gene silencing. Short hairpin RNA (shRNA) transcribed from small DNA plasmids within the target cell has also been shown to mediate stable gene silencing and achieve gene knockdown at levels comparable to those obtained by transfection with chemically synthesized siRNA (T. R. Brummelkamp, R. Bernards, R. Agami, Science 296, 550 (2002), P. J. Paddison, A. A. Caudiy, G. J. Hannon, PNAS 99, 1443 (2002)).
Possible applications of RNAi for therapeutic purposes are extensive and include silencing and knockdown of disease genes such as oncogenes or viral genes. One major obstacle for the therapeutic use of RNAi is the delivery of siRNA to the target cell (Zamore P D, Aronin N. Nature Medicine 9,(3):266-8 (2003)). In fact, delivery has been described as the major hurdle now for RNAi (Phillip Sharp, cited by Nature news feature, Vol 425, 2003, 10-12).
Therefore, new methods are needed for the safe and predictable administration of interfering RNAs to mammals.
SUMMARY OF THE INVENTION
The present invention provides at least one invasive bacterium, or at least one bacterial therapeutic particle (BTP), including one or more siRNAs or one or more DNA molecules encoding one or more siRNAs. The present invention also provides at least one prokaryotic vector including at least one DNA molecule encoding one or more siRNAs and at least one RNA-polymerase III compatible promoter or at least one prokaryotic promoter, wherein the expressed siRNAs interfere with at least one mRNA of a gene of interest.
The present invention also provides methods of using the various bacterium, BTP and vectors provided in the invention. For example, the present invention provides methods of delivering one or more siRNAs to mammalian cells. The methods include introducing at least one invasive bacterium, or at least one bacterial therapeutic particle (BTP), containing one or more siRNAs or one or more DNA molecules encoding one or more siRNAs to the mammalian cells.
The present invention also provides methods of regulating gene expression in mammalian cells. The method includes introducing at least one invasive bacterium, or at least one bacterial therapeutic particle (BTP), containing one or more siRNAs or one or more DNA molecules encoding one or more siRNAs to the mammalian cells, where the expressed siRNAs interfere with at least one mRNA of a gene of interest thereby regulating gene expression.
The present invention also provides methods of treating or preventing a viral disease or disorder in a mammal. The methods include regulating the expression of at least one gene in a cell known to cause a viral disease or disorder (e.g., known to increase proliferation, growth or dysplasia) by introducing to the cells of the mammal at least one invasive bacterium, or at least one bacterial therapeutic particle (BTP), containing one or more siRNAs or one or more DNA molecules encoding one or more siRNAs, where the expressed siRNAs interfere with the mRNA of the gene known to cause a viral disease or disorder.
Preferably, the viral disease or disorder can be, but is not limited to, infection, epithelial dysplasia and cancer caused by HPV infection
The present invention also provides a composition containing at least one invasive bacterium or BTP and a pharmaceutically acceptable carrier. The present invention also provides a eukaryotic host cell containing at least one invasive bacterium or BTP and a pharmaceutically acceptable carrier.
The invasive bacterium or BTPs of the present invention can be non-pathogenic, non-virulent bacterium or therapeutic bacterium
The mammalian cells can be ex vivo, in vivo or in vitro. The mammalian cells can be, but are not limited to, human, bovine, ovine, porcine, feline, buffalo, canine, goat, equine, donkey, deer, avian, bird, chicken, and primate cells. Preferably, the mammalian cells are human cells. In some preferred embodiments, the mammalian cells can be, but are not limited to, gastrointestinal epithelial cells, macrophages, cervical epithelial cells, rectal epithelial cells and a pharyngeal epithelial cells.
The mammalian cells can be infected with about 103 to 1011 viable invasive bacterium or BTPs (or any integer within said ranges). Preferably, the mammalian cells can be infected with about 105 to 109 viable invasive bacterium or BTPs (or any integer within said ranges). The mammalian cells can be infected at a multiplicity of infection ranging from about 0.1 to 106 (or any integer within said ranges). Preferably, the mammalian cells can be infected at a multiplicity of infection ranging from about 102 to 104 (or any integer within said ranges).
The mammal can be, but is not limited to, human, bovine, ovine, porcine, feline, buffalo, canine, goat, equine, donkey, deer, avian, bird, chicken, and primate. Preferably, the mammal is a human.
The one or more DNA molecules encoding the one or more siRNAs can be transcribed within the animal cell or transcribed within the bacterium. Preferably, the one or more siRNAs are transcribed within the animal cell as shRNAs.
The one or more DNA molecules encoding the one or more siRNAs can include one or more promoter sequences, enhancer sequences, terminator sequences, invasion factor sequences or lysis regulation sequences. The promoter can be a prokaryotic promoter. Preferably, the prokaryotic promoter is a T7 promoter, a PgapA promoter, a ParaBAD promoter, a Ptac promoter, a PlacUV5 promoter, or a recA promoter.
The expressed siRNAs can direct the multienzyme complex RNA-induced silencing complex of the cell to interact with the mRNA of one or more genes of interest. Preferably, the siRNAs interact with the mRNA of one or more HPV oncogenes. Preferably, the complex can degrade the mRNA. Preferably, the expression of one or more genes of interest is decreased or inhibited. The expression is decreased or inhibited as compared to the expression of the gene prior to administration or treatment with an invasive bacterium or BTP containing one or more siRNA or a DNA encoding for one or more siRNAs. Preferably, the expression of one or more HPV oncogenes is decreased or inhibited.
Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In the specification, the singular forms also include the plural unless the context clearly dictates otherwise. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, suitable methods and materials are described below. All publications, patent applications, patents, and other references mentioned herein are incorporated by reference. In the case of conflict, the present specification, including definitions, will control. In addition, the materials, methods, and examples are illustrative only and are not intended to be limiting.
Other features and advantages of the invention will be apparent from the following detailed description and claims.



BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 is a schematic showing the Transkingdom RNA Interference Plasmid (TRIP) with multiple hairpin express cassettes.
FIG. 2 is a schematic showing the TRIP system (bacteria and plasmid) modified with the Operator Repressor Titration (ORT) system.
FIG. 3 is a photograph showing cellular staining of the intestinal epithelial layer demonstrating efficient invasion and plasmid delivery by S. typhimurium. 
FIG. 4 is a photograph showing that bacteria-mediated RNA interference reduces target gene expression in the gastrointestinal epithelium.
FIG. 5 is a schematic showing pNJSZ plasmid construct.
FIG. 6 is a schematic showing the use of lamba S and R genes to kill intact bacteria.
FIG. 7 is a bar graph showing a reduction in HPV oncogene expression with bacterial delivered siRNA.
FIG. 8 is a bar graph showing a reduction in HPV oncogene expression with bacterial delivered siRNA.
FIG. 9 is a bar graph showing a reduction in HPV oncogene expression with bacterial delivered siRNA.
FIG. 10, Panels A-C, are a series of bar graphs showing real time PCR results following invasion of Hela cells with various siRNAs.
FIG. 11 is a photograph of an immunoblot showing the effects of downregulation of HPV E6 and E7 genes on tumor suppressor pathways and other downstream targets.
FIG. 12 is a photograph of a colony forming assay showing infection at different multiplicities of infection (MOI).
FIG. 13 is a line graph of a MTT assay showing infection at different multiplicities of infection (MOI).
FIG. 14 is a bar graph showing real time PCR results following invasion of Hela cells with various siRNAs
FIG. 15, Panels A-C, are a series of bar graphs showing real time PCR results following invasion of Hela cells with various siRNAs.
FIG. 16 is a photograph of an immunoblot showing the effects of downregulation of HPV E6 and E7 genes on tumor suppressor pathways and other downstream targets.
FIG. 17 is a bar graph showing real time PCR results following invasion assay of Hela cells with a frozen aliquot of negative sHRNA control and HPV sHRNA in BL21.
FIG. 18 is a photograph showing the plating efficiency of frozen aliquots of negative sHRNA control and HPV sHRNA in BL21.
FIG. 19 is a photograph of an immunoblot showing the knockdown of HPV E6 gene assessed by western blotting with HPV 18 E7 antibody.
FIG. 20 is a bar graph showing the knockdown of CCL20 expression with the various siRNA sequences in CMT93 cells.
FIG. 21 is a bar graph showing the knockdown of Claudin-2 expression with the various siRNA sequences in CMT93 cells post 24 hr transfection.
FIG. 22 is a bar graph showing the knockdown of IL6-RA expression with the various siRNA sequences in CMT93 cells post 24 hr transfection.
FIG. 23 is a bar graph showing the knockdown of IL13-RA1 expression with the various siRNA sequences in CMT93 cells post 24 hr transfection.
FIG. 24 is a bar graph showing the knockdown of IL18 expression with the various siRNA sequences in CMT93 cells post 24 hr transfection.
FIG. 25 is a bar graph showing the knockdown of IL-7 expression with the various siRNA sequences in CMT93 cells post 24 hr transfection.
FIG. 26 is a bar graph showing the knockdown of CH13L1 expression with the various siRNA sequences in CMT93 cells post 24 hr transfection.
FIG. 27 is a schematic of the pMBV40 or pMBV43 or pMBV44 plasmids.
FIG. 28 is a schematic of the pNJSZ ΔBamH1 plasmid.
FIG. 29 is a schematic of the pNJSZ pNJSZc plasmid.
FIG. 30 is a schematic showing a lysis regulation system in combination with strain-specific nutritional attenuation.
FIG. 31 is a schematic showing three cassettes in the combination lysis regulation system/nutritional attenuation system.
FIG. 32 is a schematic showing a lysis regulation system in combination with a Tet-on expression system.



DETAILED DESCRIPTION OF THE INVENTION
The invention pertains to compositions and methods of delivering small interfering RNAs (siRNAs) to eukaryotic cells using non-pathogenic or therapeutic strains of bacteria or bacterial therapeutic particles (BTPs). The bacteria or BTPs deliver DNA encoding siRNA, or siRNA itself, to effect RNA interference (RNAi)) by invading into the eukaryotic host cells. Generally, to trigger RNA interference in a target cell, it is required to introduce siRNA into the cell. The siRNA is either introduced into the target cell directly or by transfection or can be transcribed within the target cell as hairpin-structured dsRNA (shRNA) from specific plasmids with RNA-polymerase III compatible promoters (e.g., U6, H1) (P. J. Paddison, A. A. Caudiy, G. J. Hannon, PNAS 99, 1443 (2002), T. R. Brummelkamp, R. Bernards, R. Agami, Science 296, 550 (2002)).
The interfering RNA of the invention regulates gene expression in eukaryotic cells. It silences or knocks down genes of interest inside target cells (e.g., decreases gene activity). The interfering RNA directs the cell-owned multienzyme-complex RISC (RNA-induced silencing complex) to the mRNA of the gene to be silenced. Interaction of RISC and mRNA results in degradation or sequestration of the mRNA. This leads to effective post-transcriptional silencing of the gene of interest. This method is referred to as Bacteria Mediated Gene Silencing (BMGS).
In the case of BMGS through delivery of siRNA expressing DNA plasmids, shRNA or siRNA are produced within the target cell after liberation of the eukaryotic transcription plasmid and trigger the highly specific process of mRNA degradation, which results in silencing of the targeted gene. Additionally, one or more cell-specific eukaryotic promoters may be used that limit the expression of siRNA or shRNA to specific target cells or tissues that are in particular metabolic states. In one embodiment of this method, the cell-specific promoter is albumin and the target cell or tissue is the liver. In another embodiment of this method, the cell-specific promoter is keratin and the specific target cell or tissue is the skin.
The non-virulent bacteria and BTPs of the invention have invasive properties (or are modified to have invasive properties) and may enter a mammalian host cell through various mechanisms. In contrast to uptake of bacteria or BTPs by professional phagocytes, which normally results in the destruction of the bacterium or BTP within a specialized lysosome, invasive bacteria or BTP strains have the ability to invade non-phagocytic host cells. Naturally occurring examples of such bacteria or BTPs are intracellular pathogens such as Yersinia, Rickettsia, Legionella, Brucella, Mycobacterium, Helicobacter, Coxiella, Chlamydia, Neisseria, Burkolderia, Bordetella, Borrelia, Listeria, Shigella, Salmonella, Staphylococcus, Streptococcus, Porphyromonas, Treponema, and Vibrio, but this property can also be transferred to other bacteria or BTPs such as E. coli, Lactobacillus or Bifidobacteriae, including probiotics through transfer of invasion-related genes (P. Courvalin, S. Goussard, C. Grillot-Courvalin, C.R. Acad. Sci. Paris 318,1207 (1995)). In other embodiments of the invention, bacteria or BTPs used to deliver interfering RNAs to host cells include Shigella flexneri (D. R. Sizemore, A. A. Branstrom, J. C. Sadoff, Science 270, 299 (1995)), invasive E. coli (P. Courvalin, S. Goussard, C. Grillot-Courvalin, C.R. Acad. Sci. Paris 318,1207 (1995), C. Grillot-Courvalin, S. Goussard, F. Huetz, D. M. Ojcius, P. Courvalin, Nat Biotechnol 16, 862 (1998)), Yersinia enterocolitica (A. Al-Mariri A, A. Tibor, P. Lestrate, P. Mertens, X. De Bolle, J. J. Letesson Infect Immun 70, 1915 (2002)) and Listeria monocytogenes (M. Hense, E. Domann, S. Krusch, P. Wachholz, K. E. Dittmar, M. Rohde, J. Wehland, T. Chakraborty, S. Weiss, Cell Microbiol 3, 599 (2001), S. Pilgrim, J. Stritzker, C. Schoen, A. Kolb-Mäurer, G. Geginat, M. J. Loessner, I. Gentschev, W. Goebel, Gene Therapy 10, 2036 (2003)). Any invasive bacterium or BTP is useful for DNA transfer into eukaryotic cells (S. Weiss, T. Chakraborty, Curr Opinion Biotechnol 12, 467 (2001)).
BMGS is performed using the naturally invasive pathogen Salmonella typhimurium. In one aspect of this embodiment, the strains of Salmonella typhimurium include SL 7207 and VNP20009 (S. K. Hoiseth, B. A. D. Stocker, Nature 291, 238 (1981); Pawelek J M, Low K B, Bermudes D. Cancer Res. 57(20): 4537-44 (Oct. 15, 1997)). In another embodiment of the invention, BMGS is performed using attenuated E. coli. In another aspect of this embodiment, the CEQ201strain is engineered to possess cell-invading properties through an invasion plasmid. In one aspect of the invention, this plasmid is a TRIP (Transkingdom RNA interference plasmid) plasmid or pNJSZ.
A double “trojan horse” technique is also used with an invasive and auxotrophic bacterium or BTP carrying a eukaryotic transcription plasmid. This plasmid is, in turn, transcribed by the target cell to form one or more hairpin RNA structures that triggers the intracellular process of RNAi. This method of the invention induces significant gene silencing of a variety of genes. In certain aspects of this embodiment, the genes include a transgene (GFP), a mutated oncogene (k-Ras) and a cancer related gene (β-catenin) in vitro.
Another aspect of BMGS according to this invention is termed Transkingdom RNAi (tkRNAi). In this aspect of the invention, siRNA is directly produced by the invasive bacteria, or accumulated in the BTPs after production in the bacteria, as opposed to the target cell. A transcription plasmid controlled by a prokaryotic promoter (e.g., T7) is inserted into the carrier bacteria through standard transformation protocols. siRNA is produced within the bacteria and is liberated within the mammalian target cell after bacterial lysis triggered either by auxotrophy or by timed addition of antibiotics.
The RNAi methods of the invention, including BMGS and tkRNAi are used to create transient “knockdown” genetic animal models as opposed to genetically engineered knockout models to discover gene functions. The methods are also used as in vitro transfection tool for research and drug development
These methods use bacteria with desirable properties (invasiveness, attenuation, steerability) to perform BMGS and tkRNAi. Invasiveness as well as eukaryotic or prokaryotic transcription of one or several shRNA is conferred to a bacterium or BTP using plasmids (e.g., TRIP) and vectors as described in greater detail herein.
1. Bacterium and/or Bacterial Therapeutic Particles (BTPs)
The present invention provides at least one invasive bacterium, or at least one bacterial therapeutic particle (BTP), including one or more siRNAs or one or more DNA molecules encoding one or more siRNAs.
According to the invention, any microorganism that is capable of delivering a molecule, e.g., an RNA molecule or an RNA-encoding DNA molecule, into the cytoplasm of a target cell, such as by traversing the membrane and entering the cytoplasm of a cell, can be used to deliver RNA to such cells. In a preferred embodiment, the microorganism is a prokaryote. In an even more preferred embodiment, the prokaryote is a bacterium or BTP. Also within the scope of the invention are microorganisms other than bacteria that can be used for delivering RNA to a cell. For example, the microorganism can be a fungus, e.g., Cryptococcus neoformans, protozoan, e.g., Trypanosoma cruzi, Toxoplasma gondii, Leishmania donovani, and plasmodia. 
In a preferred embodiment, the microorganism is a bacterium or BTP. A preferred invasive bacterium or BTP is capable of delivering at least one molecule, e.g., an RNA or RNA-encoding DNA molecule, to a target cells, such as by entering the cytoplasm of a eukaryotic cell. Preferably, the RNA is siRNA or shRNA and the RNA-encoding DNA molecule encodes for siRNA or shRNA.
BTPs are fragments of bacteria used for therapeutic or preventive purposes. BTPs may include particles known in the art as minicells. Minicells are small cells produced by cell division that is faulty near the pole. They are devoid of nucleoid and, therefore, unable to grow and form colonies (Alder et al., (1967) Proc. Nat. Acad. Sci. U.S.A. 57, 321-326; for reviews see Sullivan and Maddock, (2000) Curr. Biol. 10:R249-R252; Margolin, (2001) Curr. Biol. 11, R395-R398; Howard and Kruse, (2005) J. Cell Biol. 168, 533-536). Minicell formation results due to mutations causing a defect in selection of the site for the septum formation for cell division. Such mutations include null alleles of minC, minD (Davie et al, (1984) J. Bacteriol. 158, 1202-1203; de Boer et al., 1988) J. Bacteriol. 170, 2106-2112) and certain alleles of ftsZ (Bi and Lutkenhaus, (1992) J. Bacteriol. 174, 5414-5423). Overexpression of FtsZ or MinC-MinD proteins has also been reported to cause the formation of minicells (Ward and Lutkenhaus, 1985; de Boer et al., 1988). Although minicells are devoid of nucleoid, they are capable of transcription and translation (Roozen et al., (1971) J. Bacteriol. 107, 21-33; Shepherd et al., (2001) J. Bacteriol. 183, 2527-34).
BTPs are distinct from bacteria in that they lack the bacterial genome and, therefore, provide a decreased risk of bacterial proliferation in patients. This is of particular value for immune-compromised patients. Furthermore, the inability of BTPs to proliferate allows for their use in sensitive tissues, e.g., the brain, and other areas of the body traditionally considered inaccessible to traditional siRNA. For example, the intraperitoneal delivery of bacteria can include the risk of adhesions and peritonitis, which is eliminated by utilizing BTPs. However, like the bacteria of this invention, BTPs contain the bacterial cell wall, some bacterial plasma contents and subcellular particles, one or more therapeutic components, e.g., one or more siRNAs, one or more invasion factors, one or more phagosome degradation factors, and one or more factors for targeting specific tissues. The BTPs are produced from bacteria that have produced and accumulated siRNAs inside the bacteria, and then segregate the bacterial fragment (BTP) during cell division. In one embodiment of this invention, BTPs are obtained by fermenting the bacteria, during which the BTPs form abundantly, followed by isolation of the BTPs from live bacteria using differential size filtration, which will retain the bacteria but allow passage and collection of BTPs. In another embodiment of this invention, BTPs are separated from bacteria by centrifugation. In another embodiment of this invention, live bacterial cells are lysed through activation of a death signal. Once isolated, the BTPs can be lyophilized and formulated for use.
As used herein, the term “invasive” when referring to a microorganism, e.g., a bacterium or BTP, refers to a microorganism that is capable of delivering at least one molecule, e.g., an RNA or RNA-encoding DNA molecule, to a target cell. An invasive microorganism can be a microorganism that is capable of traversing a cell membrane, thereby entering the cytoplasm of said cell, and delivering at least some of its content, e.g., RNA or RNA-encoding DNA, into the target cell. The process of delivery of the at least one molecule into the target cell preferably does not significantly modify the invasion apparatus.
Invasive microorganisms include microorganisms that are naturally capable of delivering at least one molecule to a target cell, such as by traversing the cell membrane, e.g., a eukaryotic cell membrane, and entering the cytoplasm, as well as microorganisms which are not naturally invasive and which have been modified, e.g., genetically modified, to be invasive. In another preferred embodiment, a microorganism that is not naturally invasive can be modified to become invasive by linking the bacterium or BTP to an “invasion factor”, also termed “entry factor” or “cytoplasm-targeting factor”. As used herein, an “invasion factor” is a factor, e.g., a protein or a group of proteins which, when expressed by a non-invasive bacterium or BTP, render the bacterium or BTP invasive. As used herein, an “invasion factor” is encoded by a “cytoplasm-targeting gene”.
In one embodiment of this invention, the microorganism is a naturally invasive bacterium or BTP selected from the group that includes, but is not limited to, Yersinia, Rickettsia, Legionella, Brucella, Mycobacterium, Helicobacter, Coxiella, Chlamydia, Neisseria, Burkolderia, Bordetella, Borrelia, Listeria, Shigella, Salmonella, Staphylococcus, Streptococcus, Porphyromonas, Treponema, Vibrio, E. coli, and Bifidobacteriae. Optionally, the naturally invasive bacterium or BTP is Yersinia expressing an invasion factor selected from the group including, but not limited to, invasin and YadA (Yersinia enterocolitica plasmid adhesion factor). Optionally, the naturally invasive bacterium or BTP is Rickettsia expressing the invasion factor RickA (actin polymerization protein). Optionally, the naturally invasive bacterium or BTP is Legionella expressing the invasion factor RaIF (guanine exchange factor). Optionally, the naturally invasive bacterium or BTP is Neisseria expressing an invasion factor selected from the group including, but not limited to, NadA (Neisseria adhesion/invasion factor), OpA and OpC (opacity-associated adhesions). Optionally, the naturally invasive bacterium or BTP is Listeria expressing an invasion factor selected from the group including, but not limited to, In1A (internalin factor), In1B (internalin factor), Hpt (hexose phosphate transporter), and ActA (actin polymerization protein). Optionally, the naturally invasive bacterium or BTP is Shigella expressing an invasion factor selected from the group including, but not limited to, the Shigella secreting factors IpaA (invasion plasmid antigen), IpaB, IpaC, IpgD, IpaB-IpaC complex, VirA, and IcsA. Optionally, the naturally invasive bacterium or BTP is Salmonella expressing an invasion factor selected from the group including, but not limited to, Salmonella secreting/exchange factors SipA, SipC, SpiC, SigD, SopB, SopE, SopE2, and SptP. Optionally, the naturally invasive bacterium or BTP is Staphylococcus expressing an invasion factor selected from the group including, but not limited to, the fibronectin binding proteins FnBPA and FnBPB. Optionally, the naturally invasive bacterium or BTP is Streptococcus expressing an invasion factor selected from the group including, but not limited to, the fibronectin binding proteins ACP, Fba, F2, Sfb1, Sfb2, SOF, and PFBP. Optionally, the naturally invasive bacterium or BTP is Porphyromonas gingivalis expressing the invasion factor FimB (integrin binding protein fibriae).
In another embodiment of this invention, the microorganism is a bacterium or BTP that is not naturally invasive but has been modified, e.g., genetically modified, to be invasive. Optionally, the bacterium or BTP that is not naturally invasive has been genetically modified to be invasive by expressing an invasion factor selected from the group including, but not limited to, invasin, YadA, RickA, RaIF, NadA, OpA, OpC, In1A, In1B, Hpt, ActA, IpaA, IpaB, IpaC, IpgD, IpaB-IpaC complex, VirA, IcsA, SipA, SipC, SpiC, SigD, SopB, SopE, SopE2, SptP, FnBPA, FnBPB, ACP, Fba, F2, Sfb1, Sfb2, SOF, PFBP, and FimB.
In another embodiment of this invention, the microorganism is a bacterium or BTP that may be naturally invasive but has been modified, e.g., genetically modified, to express one or more additional invasion factors. Optionally, the invasion factor is selected from the group that includes, but is not limited to, invasin, YadA, RickA, RaIF, NadA, OpA, OpC, In1A, In1B, Hpt, ActA, IpaA, IpaB, IpaC, IpgD, IpaB-IpaC complex, VirA, IcsA, SipA, SipC, SpiC, SigD, SopB, SopE, SopE2, SptP, FnBPA, FnBPB, ACP, Fba, F2, Sfb1, Sfb2, SOF, PFBP, and FimB
Naturally invasive microorganisms, e.g., bacteria or BTPs, may have a certain tropism, i.e., preferred target cells. Alternatively, microorganisms, e.g., bacteria or BTPs can be modified, e.g., genetically, to mimic the tropism of a second microorganism. Optionally, the bacterium or BTP is Streptococcus and the preferred target cells are selected from the group including, but not limited to, pharyngeal epithelial cells, buccal epithelial cells of the tongue, and mucosal epithelial cells. Optionally, the bacterium or BTP is Porphyromonas and the preferred target cells are selected from the group including, but not limited to, oral epithelial cells. Optionally, the bacterium or BTP is Staphylococcus and the preferred target cells are mucosal epithelial cells. Optionally, the bacterium or BTP is Neisseria and the preferred target cells are selected from the group including, but not limited to, urethral epithelial cells and cervical epithelial cells. Optionally, the bacterium or BTP is E. coli and the preferred target cells are selected from the group, including but not limited to, intestinal epithelial cells, urethral epithelial cells, and the cells of the upper urinary tract. Optionally, the bacterium or BTP is Bordetella and the preferred target cells are respiratory epithelial cells. Optionally, the bacterium or BTP is Vibrio and the preferred target cells are intestinal epithelial cells. Optionally, the bacterium or BTP is Treponema and the preferred target cells are mucosal epithelial cells. Optionally, the bacterium or BTP is Mycoplasma and the preferred target cells are respiratory epithelial cells. Optionally, the bacterium or BTP is Helicobacter and the preferred target cells are the endothelial cells of the stomach. Optionally, the bacterium or BTP is Chlamydia and the preferred target cells are selected from the group including, but not limited to, conjunctival cells and urethral epithelial cells.
In another embodiment of this invention, the microorganism is a bacterium or BTP that has been modified, e.g., genetically modified, to have a certain tropism. Optionally, the preferred target cells are selected from the group including, but not limited to, pharyngeal epithelial cells, buccal epithelial cells of the tongue, mucosal epithelial cells, oral epithelial cells, epithelial cells of the urethra, cervical epithelial cells, intestinal epithelial cells, respiratory epithelial cells, cells of the upper urinary tract, epithelial cells of the stomach, and conjunctival cells. Optionally, the preferred target cells are dysplastic or cancerous epithelial cells. Optionally, the preferred target cells are activated or resting immune cells.
Delivery of at least one molecule into a target cell can be determined according to methods known in the art. For example, the presence of the molecule, by the decrease in expression of an RNA or protein silenced thereby, can be detected by hybridization or PCR methods, or by immunological methods that may include the use of an antibody.
Determining whether a microorganism is sufficiently invasive for use in the invention may include determining whether sufficient siRNA was delivered to host cells, relative to the number of microorganisms contacted with the host cells. If the amount of siRNA is low relative to the number of microorganisms used, it may be desirable to further modify the microorganism to increase its invasive potential.
Bacterial or BTP entry into cells can be measured by various methods. Intracellular bacteria or BTPs survive treatment by aminoglycoside antibiotics, whereas extracellular bacteria are rapidly killed. A quantitative estimate of bacterial or BTP uptake can be achieved by treating cell monolayers with the antibiotic gentamicin to inactivate extracellular bacteria or BTPs, then by removing said antibiotic before liberating the surviving intracellular organisms with gentle detergent and determining viable counts on standard bacteriological medium. Furthermore, bacterial or BTP entry into cells can be directly observed, e.g., by thin-section-transmission electron microscopy of cell layers or by immunofluorescent techniques (Falkow et al. (1992) Annual Rev. Cell Biol. 8:333). Thus, various techniques can be used to determine whether a specific bacterium or BTP is capable of invading a specific type of cell or to confirm bacterial invasion following modification of the bacteria or BTP, such modification of the tropism of the bacteria to mimic that of a second bacterium.
Bacteria or BTPs that can be used for delivering RNA according to the method of the invention are preferably non-pathogenic. However, pathogenic bacteria or BTP s can also be used, so long as their pathogenicity has been attenuated, to thereby render the bacteria non-harmful to a subject to which it is administered. As used herein, the term “attenuated bacterium or BTP” refers to a bacterium or BTP that has been modified to significantly reduce or eliminate its harmfulness to a subject. A pathogenic bacterium or BTP can be attenuated by various methods, set forth below.
Without wanting to be limited to a specific mechanism of action, the bacterium or BTP delivering the RNA into the eukaryotic cell can enter various compartments of the cell, depending on the type of bacterium or BTP. For example, the bacterium or BTP can be in a vesicle, e.g., a phagocytic vesicle. Once inside the cell, the bacterium or BTP can be destroyed or lysed and its contents delivered to the eukaryotic cell. A bacterium or BTP can also be engineered to express a phagosome degrading protein to allow leakage of RNA from the phagosome. In one embodiment of this invention, the bacterium or BTP expresses, either naturally or through modification, e.g., genetic modification, a protein that contributes to pore-formation, breakage or degradation of the phagosome. Optionally, the protein is a cholesterol-dependent cytolysin. Optionally, the protein is selected from the group consisting of listeriolysin, ivanolysin, streptolysin, sphingomyelinase, perfringolysin, botulinolysin, leukocidin, anthrax toxin, phospholipase, IpaB (invasion plasmid antigen), IpaH, IcsB (intercellular spread), DOT/Icm (defect in organelle trafficking/intracellular multiplication defective), DOTU (stabilization factor for the DOT/Icm complex), IcmF, and PmrA (multidrug resistance efflux pump).
In some embodiments, the bacterium can stay alive for various times in the eukaryotic cell and may continue to produce RNA. The RNA or RNA-encoding DNA can then be released from the bacterium into the cell by, e.g., leakage. In certain embodiments of the invention, the bacterium can also replicate in the eukaryotic cell. In a preferred embodiment, bacterial replication does not kill the host cell. The invention is not limited to delivery of RNA or RNA-encoding DNA by a specific mechanism and is intended to encompass methods and compositions permitting delivery of RNA or RNA-encoding DNA by a bacterium independently of the mechanism of delivery. In one embodiment, the bacterium or BTP for use in the present invention is non-pathogenic or non-virulent. In another aspect of this embodiment, the bacterium or BTP is therapeutic. In another aspect of this embodiment, the bacterium or BTP is an attenuated strain or derivative thereof selected from, but not limited to, Yersinia, Rickettsia, Legionella, Brucella, Mycobacterium, Helicobacter, Haemophilus, Coxiella, Chlamydia, Neisseria, Burkolderia, Bordetella, Borrelia, Listeria, Shigella, Salmonella, Staphylococcus, Streptococcus, Porphyromonas, Treponema, Vibrio, E. coli, and Bifidobacteriae. Optionally, the Yersinia strain is an attenuated strain of the Yersinia pseudotuberculosis species. Optionally, the Yersinia strain is an attenuated strain of the Yersinia enterocolitica species. Optionally, the Rickettsia strain is an attenuated strain of the Rickettsia coronii species. Optionally, the Legionella strain is an attenuated strain of the Legionella pneumophilia species. Optionally, the Mycobacterium strain is an attenuated strain of the Mycobacterium tuberculosis species. Optionally, the Mycobacterium strain is an attenuated strain of the Mycobacterium bovis BCG species. Optionally, the Helicobacter strain is an attenuated strain of the Helicobacter pylori species. Optionally, the Coxiella strain is an attenuated strain of Coxiella burnetti. Optionally, the Haemophilus strain is an attenuated strain of the Haemophilus influenza species. Optionally, the Chlamydia strain is an attenuated strain of the Chlamydia trachomatis species. Optionally, the Chlamydia strain is an attenuated strain of the Chlamydia pneumoniae species. Optionally, the Neisseria strain is an attenuated strain of the Neisseria gonorrheae species. Optionally, the Neisseria strain is an attenuated strain of the Neisseria meningitidis species. Optionally, the Burkolderia strain is an attenuated strain of the Burkolderia cepacia species. Optionally, the Bordetella strain is an attenuated strain of the Bordetella pertussis species. Optionally, the Borrelia strain is an attenuated strain of the Borrelia hermisii species. Optionally, the Listeria strain is an attenuated strain of the Listeria monocytogenes species. Optionally, the Listeria strain is an attenuated strain of the Listeria ivanovii species. Optionally, the Salmonella strain is an attenuated strain of the Salmonella enterica species. Optionally, the Salmonella strain is an attenuated strain of the Salmonella typhimurium species. Optionally, the Salmonella typhimurium strain is SL 7207 or VNP20009. Optionally, the Staphylococcus strain is an attenuated strain of the Staphylococcus aureus species. Optionally, the Streptococcus strain is an attenuated strain of the Streptococcus pyogenes species. Optionally, the Streptococcus strain is an attenuated strain of the Streptococcus mutans species. Optionally, the Streptococcus strain is an attenuated strain of the Streptococcus salivarius species. Optionally, the Streptococcus strain is an attenuated strain of the Streptococcus pneumonia species. Optionally, the Porphyromonas strain is an attenuated strain of the Porphyromonas gingivalis species. Optionally, the Pseudomonas strain is an attenuated strain of the Pseudomonas aeruginosa species. Optionally, the Treponema strain is an attenuated strain of the Treponema pallidum species. Optionally, the Vibrio strain is an attenuated strain of the Vibrio cholerae species. Optionally, the E. coli strain is MM294.
Set forth below are examples of bacteria that have been described in the literature as being naturally invasive (section 1.1), as well as bacteria which have been described in the literature as being naturally non-invasive bacteria (section 1.2), as well as bacteria which are naturally non-pathogenic or which are attenuated. Although some bacteria have been described as being non-invasive (section 1.2), these may still be sufficiently invasive for use according to the invention. Whether traditionally described as naturally invasive or non-invasive, any bacterial strain can be modified to modulate, in particular to increase, its invasive characteristics (e.g., as described in section 1.3).
1.1 Naturally Invasive Bacteria
The particular naturally invasive bacteria employed in the present invention are not critical thereto. Examples of such naturally occurring invasive bacteria include, but are not limited to, Shigella spp., Salmonella spp., Listeria spp., Rickettsia spp., and enteroinvasive Escherichia coli. 
The particular Shigella strain employed is not critical to the present invention. Examples of Shigella strains that can be employed in the present invention include Shigella flexneri 2a (ATCC No. 29903), Shigella sonnei (ATCC No. 29930), and Shigella disenteriae (ATCC No. 13313). An attenuated Shigella strain, such as Shigella flexneri 2a 2457T aroA virG mutant CVD 1203 (Noriega et al. supra), Shigella flexneri M90T icsA mutant (Goldberg et al. Infect. Immun., 62:5664-5668 (1994)), Shigella flexneri Y SFL114 aroD mutant (Karnell et al. Vacc., 10:167-174 (1992)), and Shigella flexneri aroA aroD mutant (Verma et al. Vacc., 9:6-9 (1991)) are preferably employed in the present invention. Alternatively, new attenuated Shigella spp. strains can be constructed by introducing an attenuating mutation either singularly or in conjunction with one or more additional attenuating mutations.
At least one advantage to Shigella bacteria as delivery vectors is their tropism for lymphoid tissue in the colonic mucosal surface. In addition, the primary site of Shigella replication is believed to be within dendritic cells and macrophages, which are commonly found at the basal lateral surface of M cells in mucosal lymphoid tissues (reviewed by McGhee, J. R. et al. (1994) Reproduction, Fertility, & Development 6:369; Pascual, D. W. et al. (1994) Immunomethods 5:56). As such, Shigella vectors may provide a means to target RNA interference or deliver therapeutic molecules to these professional antigen-presenting cells. Another advantage of Shigella vectors is that attenuated Shigella strains deliver nucleic acid reporter genes in vitro and in vivo (Sizemore, D. R. et al. (1995) Science 270:299; Courvalin, P. et al. (1995) Comptes Rendus de l Academie des Sciences Serie III-Sciences de la Vie-Life Sciences 318:1207; Powell, R. J. et al. (1996) In: Molecular approaches to the control of infectious diseases. F. Brown, E. Norrby, D. Burton and J. Mekalanos, eds. Cold Spring Harbor Laboratory Press, New York. 183; Anderson, R. J. et al. (1997) Abstracts for the 97th General Meeting of the American Society for Microbiology: E.). On the practical side, the tightly restricted host specificity of Shigella stands to prevent the spread of Shigella vectors into the food chain via intermediate hosts. Furthermore, attenuated strains that are highly attenuated in rodents, primates and volunteers have been developed (Anderson et al. (1997) supra; Li, A. et al. (1992) Vaccine 10:395; Li, A. et al. (1993) Vaccine 11:180; Karnell, A. et al. (1995) Vaccine 13:88; Sansonetti, P. J. and J. Arondel (1989) Vaccine 7:443; Fontaine, A. et al. (1990) Research in Microbiology 141:907; Sansonetti, P. J. et al. (1991) Vaccine 9:416; Noriega, F. R. et al. (1994) Infection & Immunity 62:5168; Noriega, F. R. et al. (1996) Infection & Immunity 64:3055; Noriega, F. R. et al. (1996) Infection & Immunity 64:23; Noriega, F. R. et al. (1996) Infection & Immunity 64:3055; Kotloff, K. L. et al. (1996) Infection & Immunity 64:4542). This latter knowledge will allow the development of well-tolerated Shigella vectors for use in humans.
Attenuating mutations can be introduced into bacterial pathogens using non-specific mutagenesis either chemically, using agents such as N-methyl-N′-nitro-N-nitrosoguanidine, or using recombinant DNA techniques; classic genetic techniques, such as Tn10 mutagenesis, P22-mediated transduction, λ, phage mediated crossover, and conjugational transfer; or site-directed mutagenesis using recombinant DNA techniques. Recombinant DNA techniques are preferable since strains constructed by recombinant DNA techniques are far more defined. Examples of such attenuating mutations include, but are not limited to:
(i) auxotrophic mutations, such as aro (Hoiseth et al. Nature, 291:238-239 (1981)), gua (McFarland et al. Microbiol. Path., 3:129-141 (1987)), nad (Park et al. J. Bact., 170:3725-3730 (1988), thy (Nnalue et al. Infect. Immun., 55:955-962 (1987)), and asd (Curtiss, supra) mutations;
(ii) mutations that inactivate global regulatory functions, such as cya (Curtiss et al. Infect. Immun., 55:3035-3043 (1987)), crp (Curtiss et al (1987), supra), phoP/phoQ (Groisman et al. Proc. Natl. Acad. Sci., USA, 86:7077-7081 (1989); and Miller et al. Proc. Natl. Acad. Sci., USA, 86:5054-5058 (1989)), phopc (Miller et al. J. Bact., 172:2485-2490 (1990)) or ompR (Dorman et al. Infect. Immun., 57:2136-2140 (1989)) mutations;
(iii) mutations that modify the stress response, such as recA (Buchmeier et al. Mol. Micro., 7:933-936 (1993)), htrA (Johnson et al. Mol. Micro., 5:401-407 (1991)), htpR (Neidhardt et al. Biochem. Biophys. Res. Com., 100:894-900 (1981)), hsp (Neidhardt et al. Ann. Rev. Genet., 18:295-329 (1984)) and groEL (Buchmeier et al. Sci., 248:730-732 (1990)) mutations;
(iv) mutations in specific virulence factors, such as IsyA (Libby et al. Proc. Natl. Acad. Sci., USA, 91:489-493 (1994)), pag or prg (Miller et al (1990), supra; and Miller et al (1989), supra), iscA or virG (d'Hauteville et al. Mol. Micro., 6:833-841 (1992)), plcA (Mengaud et al. Mol. Microbiol., 5:367-72 (1991); Camilli et al. J. Exp. Med, 173:751-754 (1991)), and act (Brundage et al. Proc. Natl. Acad. Sci., USA, 90:11890-11894 (1993)) mutations;
(v) mutations that affect DNA topology, such as topA (Galan et al. Infect. Immun., 58:1879-1885 (1990));
(vi) mutations that disrupt or modify the cell cycle, such as min (de Boer et al. Cell, 56:641-649 (1989)).
(vii) introduction of a gene encoding a suicide system, such as sacB (Recorbet et al. App. Environ. Micro., 59:1361-1366 (1993); Quandt et al. Gene, 127:15-21 (1993)), nuc (Ahrenholtz et al. App. Environ. Micro., 60:3746-3751 (1994)), hok, gef, kil, or phlA (Molin et al. Ann. Rev. Microbiol., 47:139-166 (1993));
(viii) mutations that alter the biogenesis of lipopolysaccharide and/or lipid A, such as rFb (Raetz in Escherichia coli and Salmonella typhimurium, Neidhardt et al., Ed., ASM Press, Washington, D.C. pp 1035-1063 (1996)), galE (Hone et al. J. Infect. Dis., 156:164-167 (1987)) and htrB (Raetz, supra), msbB (Reatz, supra)
(ix) introduction of a bacteriophage lysis system, such as lysogens encoded by P22 (Rennell et al. Virol, 143:280-289 (1985)), λ murein transglycosylase (Bienkowska-Szewczyk et al. Mol. Gen. Genet., 184:111-114 (1981)) or S-gene (Reader et al. Virol, 43:623-628 (1971)); and
The attenuating mutations can be either constitutively expressed or under the control of inducible promoters, such as the temperature sensitive heat shock family of promoters (Neidhardt et al. supra), or the anaerobically induced nirB promoter (Harborne et al. Mol. Micro., 6:2805-2813 (1992)) or repressible promoters, such as uapA (Gorfinkiel et al. J. Biol. Chem., 268:23376-23381 (1993)) or gcv (Stauffer et al. J. Bact., 176:6159-6164 (1994)).
The particular Listeria strain employed is not critical to the present invention. Examples of Listeria strains that can be employed in the present invention include Listeria monocytogenes (ATCC No. 15313). Attenuated Listeria strains, such as L. monocytogenes actA mutant (Brundage et al. supra) or L. monocytogenes plcA (Camilli et al. J. Exp. Med., 173:751-754 (1991)) are preferably used in the present invention. Alternatively, new attenuated Listeria strains can be constructed by introducing one or more attenuating mutations in groups (i) to (vii) as described for Shigella spp. above.
The particular Salmonella strain employed is not critical to the present invention. Examples of Salmonella strains that can be employed in the present invention include Salmonella typhi (ATCC No. 7251) and S. typhimurium (ATCC No. 13311). Attenuated Salmonella strains are preferably used in the present invention and include S. typhi-aroC-aroD (Hone et al. Vacc. 9:810 (1991) and S. typhimurium-aroA mutant (Mastroeni et al. Micro. Pathol. 13:477 (1992)). Alternatively, new attenuated Salmonella strains can be constructed by introducing one or more attenuating mutations as described for Shigella spp. above.
The particular Rickettsia strain employed is not critical to the present invention. Examples of Rickettsia strains which can be employed in the present invention include Rickettsia Rickettsiae (ATCC Nos. VR149 and VR891), Rickettsia prowaseckii (ATCC No. VR233), Rickettsia tsutsugamuchi (ATCC Nos. VR312, VR150 and VR609), Rickettsia mooseri (ATCC No. VR144), Rickettsia sibirica (ATCC No. VR151), and Rochalimaea quitana (ATCC No. VR358). Attenuated Rickettsia strains are preferably used in the present invention and can be constructed by introducing one or more attenuating mutations in groups (i) to (vii) as described for Shigella spp. above. The particular enteroinvasive Escherichia strain employed is not critical to the present invention. Examples of enteroinvasive Escherichia strains which can be employed in the present invention include Escherichia coli strains 4608-58, 1184-68, 53638-C-17, 13-80, and 6-81 (Sansonetti et al. Ann. Microbiol. (Inst. Pasteur), 132A:351-355 (1982)). Attenuated enteroinvasive Escherichia strains are preferably used in the present invention and can be constructed by introducing one or more attenuating mutations in groups (i) to (vii) as described for Shigella spp. above.
Furthermore, since certain microorganisms other than bacteria can also interact with integrin molecules (which are receptors for certain invasion factors) for cellular uptake, such microorganisms can also be used for introducing RNA into target cells. For example, viruses, e.g., foot-and-mouth disease virus, echovirus, and adenovirus, and eukaryotic pathogens, e.g., Histoplasma capsulatum and Leishmania major interact with integrin molecules.
1.2 Less Invasive Bacteria
Examples of bacteria which can be used in the invention and which have been described in the literature as being non-invasive or at least less invasive than the bacteria listed in the previous section (1.1) include, but are not limited to, Yersinia spp., Escherichia spp., Klebsiella spp., Bordetella spp., Neisseria spp., Aeromonas spp., Francisella spp., Corynebacterium spp., Citrobacter spp., Chlamydia spp., Haemophilus spp., Brucella spp., Mycobacterium spp., Legionella spp., Rhodococcus spp., Pseudomonas spp., Helicobacter spp., Vibrio spp., Bacillus spp., and Erysipelothrix spp. It may be necessary to modify these bacteria to increase their invasive potential.
The particular Yersinia strain employed is not critical to the present invention. Examples of Yersinia strains that can be employed in the present invention include Y. enterocolitica (ATCC No. 9610) or Y. pestis (ATCC No. 19428). Attenuated Yersinia strains, such as Y. enterocolitica Ye03-R2 (al-Hendy et al. Infect. Immun., 60:870-875 (1992)) or Y. enterocolitica aroA (O'Gaora et al. Micro. Path., 9:105-116 (1990)) are preferably used in the present invention. Alternatively, new attenuated Yersinia strains can be constructed by introducing one or more attenuating mutations in groups (i) to (vii) as described for Shigella spp. above.
The particular Escherichia strain employed is not critical to the present invention. Examples of Escherichia strains that can be employed in the present invention include E. coli Nissle 1917, MM294, H10407 (Elinghorst et al. Infect. Immun., 60:2409-2417 (1992)), and E. coli EFC4, CFT325 and CPZ005 (Donnenberg et al. J. Infect. Dis., 169:831-838 (1994)). Attenuated Escherichia strains, such as the attenuated turkey pathogen E. coli 02 carAB mutant (Kwaga et al. Infect. Immun., 62:3766-3772 (1994)) or CEQ201 are preferably used in the present invention. Alternatively, new attenuated Escherichia strains can be constructed by introducing one or more attenuating mutations in groups (i) to (vii) as described for Shigella spp. above.
The particular Klebsiella strain employed is not critical to the present invention. Examples of Klebsiella strains that can be employed in the present invention include K. pneumoniae (ATCC No. 13884). Attenuated Klebsiella strains are preferably used in the present invention, and can be constructed by introducing one or more attenuating mutations in groups (i) to (vii) as described for Shigella spp. above.
The particular Bordetella strain employed is not critical to the present invention. Examples of Bordetella strains that can be employed in the present invention include B. bronchiseptica (ATCC No. 19395). Attenuated Bordetella strains are preferably used in the present invention, and can be constructed by introducing one or more attenuating mutations in groups (i) to (vii) as described for Shigella spp. above.
The particular Neisseria strain employed is not critical to the present invention. Examples of Neisseria strains that can be employed in the present invention include N. meningitidis (ATCC No. 13077) and N. gonorrhoeae (ATCC No. 19424). Attenuated Neisseria strains, such as N. gonorrhoeae MS11 aro mutant (Chamberlain et al. Micro. Path., 15:51-63 (1993)) are preferably used in the present invention. Alternatively, new attenuated Neisseria strains can be constructed by introducing one or more attenuating mutations in groups (i) to (vii) as described for Shigella spp. above. The particular Aeromonas strain employed is not critical to the present invention. Examples of Aeromonas strains that can be employed in the present invention include A. eucrenophila (ATCC No. 23309). Alternatively, new attenuated Aeromonas strains can be constructed by introducing one or more attenuating mutations in groups (i) to (vii) as described for Shigella spp. above.
The particular Francisella strain employed is not critical to the present invention. Examples of Francisella strains that can be employed in the present invention include F. tularensis (ATCC No. 15482). Attenuated Francisella strains are preferably used in the present invention, and can be constructed by introducing one or more attenuating mutations in groups (i) to (vii) as described for Shigella spp. above.
The particular Corynebacterium strain employed is not critical to the present invention. Examples of Corynebacterium strains that can be employed in the present invention include C. pseudotuberculosis (ATCC No. 19410). Attenuated Corynebacterium strains are preferably used in the present invention, and can be constructed by introducing one or more attenuating mutations in groups (i) to (vii) as described for Shigella spp. above.
The particular Citrobacter strain employed is not critical to the present invention. Examples of Citrobacter strains that can be employed in the present invention include C. freundii (ATCC No. 8090). Attenuated Citrobacter strains are preferably used in the present invention, and can be constructed by introducing one or more attenuating mutations in groups (i) to (vii) as described for Shigella spp. above.
The particular Chlamydia strain employed is not critical to the present invention. Examples of Chlamydia strains that can be employed in the present invention include C. pneumoniae (ATCC No. VR1310). Attenuated Chlamydia strains are preferably used in the present invention, and can be constructed by introducing one or more attenuating mutations in groups (i) to (vii) as described for Shigella spp. above.
The particular Haemophilus strain employed is not critical to the present invention. Examples of Haemophilus strains that can be employed in the present invention include H. sornnus (ATCC No. 43625). Attenuated Haemophilus strains are preferably used in the present invention, and can be constructed by introducing one or more attenuating mutations in groups (i) to (vii) as described for Shigella spp. above.
The particular Brucella strain employed is not critical to the present invention. Examples of Brucella strains that can be employed in the present invention include B. abortus (ATCC No. 23448). Attenuated Brucella strains are preferably used in the present invention, and can be constructed by introducing one or more attenuating mutations in groups (i) to (vii) as described for Shigella spp. above.
The particular Mycobacterium strain employed is not critical to the present invention. Examples of Mycobacterium strains that can be employed in the present invention include M. intracellulare (ATCC No. 13950) and M. tuberculosis (ATCC No. 27294). Attenuated Mycobacterium strains are preferably used in the present invention, and can be constructed by introducing one or more attenuating mutations in groups (i) to (vii) as described for Shigella spp. above.
The particular Legionella strain employed is not critical to the present invention. Examples of Legionella strains that can be employed in the present invention include L. pneumophila (ATCC No. 33156). Attenuated Legionella strains, such as a L. pneumophila mip mutant (Ott, FEMS Micro. Rev., 14:161-176 (1994)) are preferably used in the present invention. Alternatively, new attenuated Legionella strains can be constructed by introducing one or more attenuating mutations in groups (i) to (vii) as described for Shigella spp. above.
The particular Rhodococcus strain employed is not critical to the present invention. Examples of Rhodococcus strains that can be employed in the present invention include R. equi (ATCC No. 6939). Attenuated Rhodococcus strains are preferably used in the present invention, and can be constructed by introducing one or more attenuating mutations in groups (i) to (vii) as described for Shigella spp. above.
The particular Pseudomonas strain employed is not critical to the present invention. Examples of Pseudomonas strains that can be employed in the present invention include P. aeruginosa (ATCC No. 23267). Attenuated Pseudomonas strains are preferably used in the present invention, and can be constructed by introducing one or more attenuating mutations in groups (i) to (vii) as described for Shigella spp. above.
The particular Helicobacter strain employed is not critical to the present invention. Examples of Helicobacter strains that can be employed in the present invention include H. mustelae (ATCC No. 43772). Attenuated Helicobacter strains are preferably used in the present invention, and can be constructed by introducing one or more attenuating mutations in groups (i) to (vii) as described for Shigella spp. above.
The particular Salmonella strain employed is not critical to the present invention. Examples of Salmonella strains that can be employed in the present invention include Salmonella typhi (ATCC No. 7251) and S. typhimurium (ATCC No. 13311). Attenuated Salmonella strains are preferably used in the present invention and include S. typhi aroC aroD (Hone et al. Vacc., 9:810-816 (1991)) and S. typhimurium aroA mutant (Mastroeni et al. Micro. Pathol, 13:477-491 (1992))). Alternatively, new attenuated Salmonella strains can be constructed by introducing one or more attenuating mutations in groups (i) to (vii) as described for Shigella spp. above.
The particular Vibrio strain employed is not critical to the present invention. Examples of Vibrio strains that can be employed in the present invention include Vibrio cholerae (ATCC No. 14035) and Vibrio cincinnatiensis (ATCC No. 35912). Attenuated Vibrio strains are preferably used in the present invention and include V. cholerae RSI virulence mutant (Taylor et al. J. Infect. Dis., 170:1518-1523 (1994)) and V. cholerae ctxA, ace, zot, cep mutant (Waldor et al. J. Infect. Dis., 170:278-283 (1994)). Alternatively, new attenuated Vibrio strains can be constructed by introducing one or more attenuating mutations in groups (i) to (vii) as described for Shigella spp. above.
The particular Bacillus strain employed is not critical to the present invention. Examples of Bacillus strains that can be employed in the present invention include Bacillus subtilis (ATCC No. 6051). Attenuated Bacillus strains are preferably used in the present invention and include B. anthracis mutant pX01 (Welkos et al. Micro. Pathol, 14:381-388 (1993)) and attenuated BCG strains (Stover et al. Nat., 351:456-460 (1991)). Alternatively, new attenuated Bacillus strains can be constructed by introducing one or more attenuating mutations in groups (i) to (vii) as described for Shigella spp. above.
The particular Erysipelothrix strain employed is not critical to the present invention. Examples of Erysipelothrix strains that can be employed in the present invention include Erysipelothrix rhusiopathiae (ATCC No. 19414) and Erysipelothrix tonsillarum (ATCC No. 43339). Attenuated Erysipelothrix strains are preferably used in the present invention and include E. rhusiopathiae Kg-1a and Kg-2 (Watarai et al. J. Vet. Med. Sci., 55:595-600 (1993)) and E. rhusiopathiae ORVAC mutant (Markowska-Daniel et al. Int. J. Med. Microb. Virol. Parisit. Infect. Dis., 277:547-553 (1992)). Alternatively, new attenuated Erysipelothrix strains can be constructed by introducing one or more attenuating mutations in groups (i) to (vii) as described for Shigella spp. above.
1.3. Methods for Increasing the Invasive Properties of a Bacterial Strain
Whether organisms have been traditionally described as invasive or non-invasive, these organisms can be engineered to increase their invasive properties, e.g., by mimicking the invasive properties of Shigella spp., Listeria spp., Rickettsia spp., or enteroinvasive E. coli spp. For example, one or more genes that enable the microorganism to access the cytoplasm of a cell, e.g., a cell in the natural host of said non-invasive bacteria, can be introduced into the microorganism.
Examples of such genes referred to herein as “cytoplasm-targeting genes” include genes encoding the proteins that enable invasion by Shigella or the analogous invasion genes of entero-invasive Escherichia, or listeriolysin O of Listeria, as such techniques are known to result in rendering a wide array of invasive bacteria capable of invading and entering the cytoplasm of animal cells (Formal et al. Infect. Immun., 46:465 (1984); Bielecke et al. Nature, 345:175-176 (1990); Small et al. In: Microbiology-1986, pages 121-124, Levine et al. Eds., American Society for Microbiology, Washington, D.C. (1986); Zychlinsky et al. Molec. Micro., 11:619-627 (1994); Gentschev et al. (1995) Infection & Immunity 63:4202; Isberg, R. R. and S. Falkow (1985) Nature 317:262; and Isberg, R. R. et al. (1987) Cell 50:769). Methods for transferring the above cytoplasm-targeting genes into a bacterial strain are well known in the art. Another preferred gene that can be introduced into bacteria to increase their invasive character encodes the invasin protein from Yersinia pseudotuberculosis, (Leong et al. EMBO J., 9:1979 (1990)). Invasin can also be introduced in combination with listeriolysin, thereby further increasing the invasive character of the bacteria relative to the introduction of either of these genes. The above genes have been described for illustrative purposes; however, it will be obvious to those skilled in the art that any gene or combination of genes, from one or more sources, that participates in the delivery of a molecule, in particular an RNA or RNA-encoding DNA molecule, from a microorganism into the cytoplasm of a cell, e.g., an animal cell, will suffice. Thus, such genes are not limited to bacterial genes, and include viral genes, such as influenza virus hemagglutinin HA-2 that promotes endosmolysis (Plank et al. J. Biol. Chem., 269:12918-12924 (1994)).
The above cytoplasm-targeting genes can be obtained by, e.g., PCR amplification from DNA isolated from an invasive bacterium carrying the desired cytoplasm-targeting gene. Primers for PCR can be designed from the nucleotide sequences available in the art, e.g., in the above-listed references and/or in GenBank, which is publicly available on the internet (www.ncbi.nlm.nih.gov/). The PCR primers can be designed to amplify a cytoplasm-targeting gene, a cytoplasm-targeting operon, a cluster of cytoplasm-targeting genes, or a regulon of cytoplasm-targeting genes. The PCR strategy employed will depend on the genetic organization of the cytoplasm-targeting gene or genes in the target invasive bacteria. The PCR primers are designed to contain a sequence that is homologous to DNA sequences at the beginning and end of the target DNA sequence. The cytoplasm-targeting genes can then be introduced into the target bacterial strain, e.g., by using Hfr transfer or plasmid mobilization (Miller, A Short Course in Bacterial Genetics, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1992); Bothwell et al. supra; and Ausubel et al. supra), bacteriophage-mediated transduction (de Boer, supra; Miller, supra; and Ausubel et al. supra), chemical transformation (Bothwell et al. supra; Ausubel et al. supra), electroporation (Bothwel et al. supra; Ausubel et al. supra; and Sambrook, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.) and physical transformation techniques (Johnston et al. supra; and Bothwell, supra). The cytoplasm-targeting genes can be incorporated into lysogenic bacteriophage (de Boer et al. Cell, 56:641-649 (1989)), plasmids vectors (Curtiss et al. supra) or spliced into the chromosome (Hone et al. supra) of the target strain.
In addition to genetically engineering bacteria and BTPs to increase their invasive properties, as set forth above, bacteria and can also be modified by linking an invasion factor to the bacteria. Accordingly, in one embodiment, a bacterium is rendered more invasive by coating the bacterium, either covalently or non-covalently, with an invasion factor, e.g., the protein invasin, invasin derivatives, or a fragment thereof sufficient for invasiveness. In fact, it has been shown that non-invasive bacterial cells coated with purified invasin from Yersinia pseudotuberculosis or the carboxyl-terminal 192 amino acids of invasin are able to enter mammalian cells (Leong et al. (1990) EMBO J. 9:1979). Furthermore, latex beads coated with the carboxyl terminal region of invasin are efficiently internalized by mammalian cells, as are strains of Staphylococcus aureus coated with antibody-immobilized invasin (reviewed in Isberg and Tran van Nhieu (1994) Ann. Rev. Genet. 27:395). Alternatively, a bacterium can also be coated with an antibody, variant thereof, or fragment thereof, which binds specifically to a surface molecule recognized by a bacterial entry factor. For example, it has been shown that bacteria are internalized if they are coated with a monoclonal antibody directed against an integrin molecule, e.g., α5β1, known to be the surface molecule with which the bacterial invasin protein interacts (Isberg and Tran van Nhieu, supra). Such antibodies can be prepared according to methods known in the art. The antibodies can be tested for efficacy in mediating bacterial invasiveness by, e.g., coating bacteria with the antibody, contacting the bacteria with eukaryotic cells having a surface receptor recognized by the antibody, and monitoring the presence of intracellular bacteria, according to the methods described above. Methods for linking an invasion factor to the surface of a bacterium are known in the art and include cross-linking.
3. Plasmids and Vectors
The present invention also provides at least one vector or plasmid including at least one DNA molecule encoding one or more siRNAs and at least one promoter, wherein the expressed siRNAs interfere with at least one mRNA of a gene of interest. In one preferred embodiment, the present invention provides at least one prokaryotic vector including at least one DNA molecule encoding one or more siRNAs and at least one RNA-polymerase III compatible promoter or at least one prokaryotic promoter, wherein the expressed siRNAs interfere with at least one mRNA of a gene of interest.
The TRIP (transkingdom RNA interference plasmid) vectors and plasmids of the present invention include a multiple cloning site, a promoter sequence and a terminator sequence. The TRIP vectors and plasmids also include one or more sequences encoding for an invasion factor to permit the non-invasive bacterium or BTP to enter mammalian cells (e.g., the Inv locus that encodes invasion that permits the bacterium or BTP to enter β1-integrin-positive mammalian cells) (Young et al., J. Cell Biol. 116, 197-207 (1992)) and one or more sequences to permit the genetic material to escape from the entry vesicles (e.g., Hly A gene that encodes listeriolysin O) (Mathew et al., Gene Ther. 10, 1105-1115 (2003) and Grillot-Courvalin et al., Nat. Biotechnol. 16, 862-866 (1998)). TRIP is further described (including a vector/plasmid schematic) in PCT Publication No. WO 06/066048. In preferred embodiments, the TRIP vectors and plasmids will incorporate a hairpin RNA expression cassette encoding short hairpin RNA under the control of an appropriate promoter sequence and terminator sequence.
In the design of these constructs, an algorithm was utilized to take into account some known difficulties with the development of siRNA, namely: (1) Exclusion of disqualifying properties (SNPs, interferon motifs); (2) Exclusion of the sequence if there was homology in ref seq (19/21, >17 contiguous to any other genes) and (3) Exclusion of the sequence if there were significant miRNA seed type matches.
As described herein, the one or more DNA molecules encoding the one or more siRNAs are transcribed within the eukaryotic target cell or transcribed within the bacterium or BTP.
In embodiments where the DNA is transcribed within the eukaryotic cell, the one or more siRNAs are transcribed within the eukaryotic cells as shRNAs. The eukaryotic cell can be in vivo, in vitro or ex vivo. In one aspect of this embodiment, the one or more DNA molecules encoding the one or more siRNAs contain a eukaryotic promoter. Optionally, the eukaryotic promoter is a RNA polymerase III promoter. Optionally, the RNA polymerase III promoter is a U6 promoter or an H1 promoter.
In embodiments where the DNA is transcribed within the bacterium or BTP, the one or more DNA molecules contain a prokaryotic promoter. Optionally, the prokaryotic promoter is an E. coli promoter. Preferably, the E. coli promoter can be a T7 promoter, lacUV5 promoter, RNA polymerase promoter, gapA promoter, pA1 promoter, lac regulated promoter, araC+ParaBAD promoter, T5 promoter, Ptac promoter (Estrem et al, 1998, Proc. Natl. Acad. Sci. USA 95, 9761-9766; Meng et al., 2001, Nucleic Acids Res. 29, 4166-417; De Boer et al., 1983, Proc. NatL Acad. Sci. USA 80, 21-25) or recA promoter.
Preferable, promoter sequences are recited in Table 1.









TABLE 1





Promoter
Sequence
SEQ ID NO:

















T7 promoter
TAATACGACTCACTATAG
1


 


lacUV5 promoter
TAACCAGGCTTTACACTTTATG
2



CTTCCGGCTCGTATAATGTGTG




GAAGGATCC



 


RNA polymerase
TAACCAGGCTTTACACTTTATG
3


promoter
CTTCCGGCTCGTATAATGTGTG




GAA



 


RNA polymerase
TAAAATTCAAAAATTTATTTGC
4


promoter
TTTCAGGAAAATTTTTCTGTAT




AATAGATTC



 


RNA polymerase
TAATTGATACTTTATGCTTTTT
5


promoter
TCTGTATAAT



 


gapA promoter
AAGCTTTCAGTCGCGTAATGCT
6



TAGGCACAGGATTGATTTGTCG




CAATGATTGACACGATTCCGCT




TGACACTGCGTAAGTTTTGTGT




TATAATGGATCC



 


pA1 promoter
AAGCTTAAGGAGAGACAACTTA
7



AAGAGACTTAAAAGATTAATTT




AAAATTTATCAAAAAGAGTATT




GACTTAAAGTCTAACCTATAGG




ATACTTGGATCC



 


lac regulated
AAGCTTTGTGTGGAATTGTGAG
8


promoter
CGGATAACAATTCCACACATTG




ACACTTTATGCTTCCGGCTCGT




ATAATGGATCC



 


lac regulated
AAGCTTGGAAAATTTTTTTTAA
9


promoter
AAAAGTCATGTGTGGAATTGTG




AGCGGATAACAATTCCACATAT




AATGGATCC



 


araC+ ParaBAD
GACTTCATATACCCAAGCTTTA
10


promoter
AAAAAAAAATCCTTAGCTTTCG




CTAAGGATCTCCGTCAAGCCGT




CAATTGTCTGATTCGTTACCAA




TTATGACAACTTGACGGCTACA




TCATTCACTTTTTCTTCACAAC




CGGCACGAAACTCGCTCGGGCT




GGCCCCGGTGCATTTTTTAAAT




ACTCGCGAGAAATAGAGTTGAT




CGTCAAAACCAACATTGCGACC




GACGGTGGCGATAGGCATCCGG




GTAGTGCTCAAAAGCAGCTTCG




CCTGACTAATGCGTTGGTCCTC




GCGCCAGCTTAAGACGCTAATC




CCTAACTGCTGGCGGAAAAGAT




GTGACAGACGCGACGGCGACAA




GCAAACATGCTGTGCGACGCTG




GCGATATCAAAATTGCTGTCTG




CCAGGTGATCGCTGATGTACTG




ACAAGCCTCGCGTACCCGATTA




TCCATCGGTGGATGGAGCGACT




CGTTAATCGCTTCCATGCGCCG




CAGTAACAATTGCTCAAGCAGA




TTTATCGCCAGCAGCTCCGAAT




AGCGCCCTTCCCCTTGCCCGGC




GTTAATGATTTGCCCAAACAGG




TCGCTGAAATGCGGCTGGTGCG




CTTCATCCGGGCGAAAGAAACC




CGTATTGGCAAATATTGACGGC




CAGTTAAGCCATTCATGCCAGT




AGGCGCGCGGACGAAAGTAAAC




CCACTGGTGATACCATTCGCGA




GCCTCCGGATGACGACCGTAGT




GATGAATCTCTCCTGGCGGGAA




CAGCAAAATATCACCCGGTCGG




CAGACAAATTCTCGTCCCTGAT




TTTTCACCACCCCCTGACCGCG




AATGGTGAGATTGAGAATATAA




CCTTTCATTCCCAGCGGTCGGT




CGATAAAAAAATCGAGATAACC




GTTGGCCTCAATCGGCGTTAAA




CCCGCCACCAGATGGGCGTTAA




ACGAGTATCCCGGCAGCAGGGG




ATCATTTTGCGCTTCAGCCATA




CTTTTCATACTCCCACCATTCA




GAGAAGAAACCAATTGTCCATA




TTGCATCAGACATTGCCGTCAC




TGCGTCTTTTACTGGCTCTTCT




CGCTAACCCAACCGGTAACCCC




GCTTATTAAAAGCATTCTGTAA




CAAAGCGGGACCAAAGCCATGA




CAAAAACGCGTAACAAAAGTGT




CTATAATCACGGCAGAAAAGTC




CACATTGATTATTTGCACGGCG




TCACACTTTGCTATGCCATAGC




ATTTTTATCCATAAGATTAGCG




GATCCTACCTGACGCTTTTTAT




CGCAACTCTCTACTGTAGATCT




ATCTGCGAT



 


T5 promoter
TAAAATTCAAAAATTTATTTGC
11



TTTCAGGAAAATTTTTCTGTAT




AATAGATTCGGATCC



 


recA promoter
TAATTGATACTTTATGCTTTTT
12



TCTGTATAATGGATCC



 


Ptac promoter
GACTTCATATACCCAAGCTTGG
378



AAAATTTTTTTTAAAAAAGTCT




TGACACTTTATGCTTCCGGCTC




GTATAATGGATCC



 


Patac promoter
GGAAAATTTTTTTTAAAAAAGT
379



C









In embodiments where the DNA is transcribed within the bacterium or BTP, the E. coli promoter is associated with a terminator. Preferably, the E. coli terminator can be a T7 terminator, lacUV5 terminator, Rho-independent terminator, Rho-dependent terminator, or RNA polymerase terminator.
Preferable, terminator sequences are recited in Table 2.









TABLE 2





Terminator
Sequence
SEQ ID NO:







T7 terminator
TAGCATAACCCCTTGGGGCCTC
13



TAAACGGGTCTTGAGGGGTTTT




TTG



 


lacUV5
TTGTCACGTGAGCGGATAACAA
14


terminator
TTTCACACAGGAAACAGAATTC




TTAAT



 


Rho-independent
TTGTCACAAACCCCGCCACCGG
15


terminator
CGGGGTTTTTTTCTGCTTAAT



 


Rho-dependent
TTGTCACAATTCTATGGTGTAT
16


terminator
GCATTTATTTGCATACATTCAA




TCAATTGGATCCTGCATTAAT



 


RNA polymerase
GTGAGCGGATAACAATTTCACA
17


terminator
CAGGAAACAGAATTCTTAAT



 


RNA polymerase
AAACCCCGCCACCGGCGGGGTT
18


terminator
TTTTTCTGCTTAAT



 


RNA polymerase
AATTCTATGGTGTATGCATTTA
19


terminator
TTTGCATACATTCAATCAATTG




GATCCTGCATTAAT









In additional embodiments, the vectors and plasmids of the present invention further include one or more enhancer sequences, selection markers, or lysis regulation system sequences.
In one aspect of the invention, the one or more DNA molecules contain a prokaryotic enhancer. Optionally, the prokaryotic enhancer is a T7 enhancer. Optionally, the T7 enhancer has the sequence GAGACAGG (SEQ ID NO: 563). In another aspect of this embodiment, the one or more DNA molecules contain a prokaryotic terminator.
In another aspect of the, the one or more DNA molecules are associated with one or more selection markers. In one aspect of this embodiment, the selection marker is an amber suppressor containing one or more mutations or an diamino pimelic acid (DAP) containing one or more mutations. Optionally, the dap gene is selected from, but not limited to, dapA and dapE.
Preferable, selection marker sequences are recited in Table 3.









TABLE 3





Selection Marker
Sequence
SEQ ID NO:







amber suppressor
AATTCGGGGCTATAGCTCAGCT
20


gene sequence
GGGAGAGCGCTTGCATCTAATG




CAAGAGGTCAGCGGTTCGATCC




CGCTTAGCTCCACCACTGCA



 


amber suppressor
AATTCGCCCGGATAGCTCAGTC
21


sequence
GGTAGAGCAGGGGATTCTAAAT




CCCCGTGTCCTTGGTTCGATTC




CGAGTCCGGGCACTGCA



 


Rho- lgt with
ATGACCAGTAGCTATCTGCATT
22


double
AGCCGGAGTAGGATCCGGTCAT



amber mutation
TTTCTCAATAGGACCCGTGGCG



(lgt am-am
CTTCACTGGTACGGCCTGATGT



allele
ATCTGGTGGGTTTCATTTTTGC



of lgt gene)
AATGTGGCTGGCAACACGACGG



sequence
GCGAATCGTCCGGGCAGCGGCT




GGACCAAAAATGAAGTTGAAAA




CTTACTCTATGCGGGCTTCCTC




GGCGTCTTCCTCGGGGGACGTA




TTGGTTATGTTCTGTTCTACAA




TTTCCCGCAGTTTATGGCCGAT




CCGCTGTATCTGTTCCGTGTCT




GGGACGGCGGCATGTCTTTCCA




CGGCGGCCTGATTGGCGTTATC




GTGGTGATGATTATCTTCGCCC




GCCGTACTAAACGTTCCTTCTT




CCAGGTCTCTGATTTTATCGCA




CCACTCATTCCGTTTGGTCTTG




GTGCCGGGCGTCTGGGCAACTT




TATTAACGGTGAATTGTGGGGC




CGCGTTGACCCGAACTTCCCGT




TTGCCATGCTGTTCCCTGGCTC




CCGTACAGAAGATATTTTGCTG




CTGCAAACCAACCCGCAGTGGC




AATCCATTTTCGACACTTACGG




TGTGCTGCCGCGCCACCCATCA




CAGCTTTACGAGCTGCTGCTGG




AAGGTGTGGTGCTGTTTATTAT




CCTCAACCTGTATATTCGTAAA




CCACGCCCAATGGGAGCTGTCT




CAGGTTTGTTCCTGATTGGTTA




CGGCGCGTTTCGCATCATTGTT




GAGTTTTTCCGCCAGCCCGACG




CGCAGTTTACCGGTGCCTGGGT




GCAGTACATCAGCATGGGGCAA




ATTCTTTCCATCCCGATGATTG




TCGCGGGTGTGATCATGATGGT




CTGGGCATATCGTCGCAGCCCA




CAGCAACACGTTTCCTGA



 


murA with double
ATGGATAAATTTCGTGTTCAGG
23


amber
GGCCAACGAAGCTCCAGGGCGA



mutation (murA
AGTCACAATTTCCGGCGCTAAA



am-am allele
AATTAGTAGCTGCCTATCCTTT



of murA gene)
TTGCCGCACTACTGGCGGAAGA



sequence
ACCGGTAGAGATCCAGAACGTC




CCGAAACTGAAAGACGTCGATA




CATCAATGAAGCTGCTAAGCCA




GCTGGGTGCGAAAGTAGAACGT




AATGGTTCTGTGCATATTGATG




CCCGCGACGTTAATGTATTCTG




CGCACCTTACGATCTGGTTAAA




ACCATGCGTGCTTCTATCTGGG




CGCTGGGGCCGCTGGTAGCGCG




CTTTGGTCAGGGGCAAGTTTCA




CTACCTGGCGGTTGTACGATCG




GTGCGCGTCCGGTTGATCTACA




CATTTCTGGCCTCGAACAATTA




GGCGCGACCATCAAACTGGAAG




AAGGTTACGTTAAAGCTTCCGT




CGATGGTCGTTTGAAAGGTGCA




CATATCGTGATGGATAAAGTCA




GCGTTGGCGCAACGGTGACCAT




CATGTGTGCTGCAACCCTGGCG




GAAGGCACCACGATTATTGAAA




ACGCAGCGCGTGAACCGGAAAT




CGTCGATACCGCGAACTTCCTG




ATTACGCTGGGTGCGAAAATTA




GCGGTCAGGGCACCGATCGTAT




CGTCATCGAAGGTGTGGAACGT




TTAGGCGGCGGTGTCTATCGCG




TTCTGCCGGATCGTATCGAAAC




CGGTACTTTCCTGGTGGCGGCG




GCGATTTCTCGCGGCAAAATTA




TCTGCCGTAACGCGCAGCCAGA




TACTCTCGACGCCGTGCTGGCG




AAACTGCGTGACGCTGGAGCGG




ACATCGAAGTCGGCGAAGACTG




GATTAGCCTGGATATGCATGGC




AAACGTCCGAAGGCTGTTAACG




TACGTACCGCGCCGCATCCGGC




ATTCCCGACCGATATGCAGGCC




CAGTTCACGCTGTTGAACCTGG




TGGCAGAAGGGACCGGGTTTAT




CACCGAAACGGTCTTTGAAAAC




CGCTTTATGCATGTGCCAGAGC




TGAGCCGTATGGGCGCGCACGC




CGAAATCGAAAGCAATACCGTT




ATTTGTCACGGTGTTGAAAAAC




TTTCTGGCGCACAGGTTATGGC




AACCGATCTGCGTGCATCAGCA




AGCCTGGTGCTGGCTGGCTGTA




TTGCGGAAGGGACGACGGTGGT




TGATCGTATTTATCACATCGAT




CGTGGCTACGAACGCATTGAAG




ACAAACTGCGCGCTTTAGGTGC




AAATATTGAGCGTGTGAAAGGC




GAATAA



 


dapA sequence
GCCAGGCGACTGTCTTCAATAT
24



TACAGCCGCAACTACTGACATG




ACGGGTGATOGTGTTCACAATT




CCAGGGCGATCGGCACCCAACG




CAGTGATCACCAGATAATGTTG




CGATGACAGTGTCAAACTGGTT




ATTCCTTTAAGGGGTGAGTTGT




TCTTAAGGAAAGCATAAAAAAA




ACATGCATACAACAATCAGAAC




GGTTCTGTCTGCTTGCTTTTAA




TGCCATACCAAACGTACCATTG




AGACACTTGTTTGCACAGAGGA




TGGCCCATGTTCACGGGAAGTA




TTGTCGCGATTGTTACTCCGAT




GGATGAAAAAGGTAATGTCTGT




CGGGGTAGCTTGAAAAAACTGA




TTGATTATCATGTCGCCAGCGG




TACTTCGGCGATCGTTTCTGTT




GGCACCACTGGCGAGTCCGCTA




CCTTAAATCATGACGAACATGC




TGATGTGGTGATGATGACGCTG




GATGTGGCTGATGGGCGCATTC




CGGTAATTGCCGGGACCGGCGC




TAACGCTACTGCGGAAGCCATT




AGCCTGACGCAGCGCTTCAATG




ACAGTGGTATCGTCGGCTGCCT




GACGGTAACCCCTTACTACAAT




CGTCCGTCGCAAGAAGGTTTGT




ATCAGCATTTCAAAGCCATCGC




TGAGCATACTGACCTGCCGCAA




ATTCTGTATAATGTGCCGTCCC




GTACTGGCTGCGATCTGCTCCC




GGAAACGGTGGGCCGTCTGGCG




AAAGTAAAAAATATTATCGGAA




TCAAAGAGGCAACAGGGAACTT




AACGCGTGTAAACCAGATCAAA




GAGCTGGTTTCAGATGATTTTG




TTCTGCTGAGCGGCGATGATGC




GAGCGCGCTGGACTTCATGCAA




TTGGGCGGTCATGGGGTTATTT




CCGTTACGGCTAACGTCGCAGC




GCGTGATATGGGCCAGATGTGC




AAACTGGCAGCAGAAGGGCATT




TTGCCGAGGCACGCGTTATTAA




TCAGCGTCTGATGCCATTACAC




AACAAACTATTTGTCGAACCCA




ATCCAATCCCGGTGAAATGGGC




ATGTAAGGAACTGGGTCTTGTG




GCGACCGATACGCTGCGCCTGC




CAATGACACCAATCACCGACAG




TGGTCGTGAGACGGTCAGAGCG




GCGCTTAAGCATGCCGGTTTGC




TGTAAAGTTTAGGGAGATTTGA




TGGCTTACTCTGTTCAAAAGTC




GCGCCTGGCAAAGGTTGCGGGT




GTTTCGCTTGTTTTATTACTCG




CTGCCTGTAGTTCTGACTCACG




CTATAAGCGTCAGGTCAGTGGT




GATGAAGCCTACCTGGAAGCG









Optionally, the amber suppressor is associated with a promoter or a terminator. Optionally, the promoter is a lipoprotein promoter. Preferable, promoter sequences are recited in Table 4.









TABLE 4





Amber Suppressor

SEQ ID


Promoter Sequence
Sequence
NO:







lipoprotein promoter
CATGGCGCCGCTTCTTTGAGCG
25



AACGATCAAAAATAAGTGGCGC




CCCATCAAAAAAATATTCTCAA




CATAAAAAACTTTGTGTAATAC




TTGTAACGCTG



 


lipoprotein promoter
CATGGCGCCCCATCAAAAAAAT
26



ATTCTCAACATAAAAAACTTTG




TGTAATACTTGTAACGCTG









Optionally, the terminator is an rrnC terminator. Preferable, terminator sequences are recited in Table 5.









TABLE 5





Amber Suppressor

SEQ ID


Terminator Sequence
Sequence
NO:







rrnC terminator
GATCCTTAGCGAAAGCTAAGGA
27



TTTTTTTTAC



 


rrnC terminator
GATCCTTAGCGAAAGCTAAGGA
28



TTTTTTTTTT









Bacterial and BTP delivery is more attractive than viral delivery because they are more accessible to genetic manipulation, which allows the production of vector strains specifically tailored to certain applications. In one embodiment of the invention, the methods of the invention are used to create bacteria and BTPs that cause RNAi in a tissue specific manner.
Liberation of the siRNA encoding plasmid or the one or more siRNAs from the intracellular bacteria or BTPs occurs through active mechanisms. One mechanism involves the type III export system in S. typhimurium, a specialized multiprotein complex spanning the bacterial or BTP cell membrane whose functions include secretion of virulence factors to the outside of the cell to allow signaling towards the target cell, but which can also be used to deliver antigens into target cells (Rüssmann H. Int J Med Microbiol, 293:107-12 (2003)), or through bacterial lysis and liberation of bacterial or BTP contents into the cytoplasm. The lysis of intracellular bacteria or BTPs is triggered through various mechanisms, including addition of an intracellularly active antibiotic (tetracycline), naturally through bacterial metabolic attenuation (auxotrophy), or through a lysis regulation system or bacterial suicide system comprising a bacterial regulator, promoter and sensor that is sensitive to the environment, e.g., the pH, magnesium concentration, phosphate concentration, ferric ion concentration, osmolarity, anaerobic conditions, nutritional deficiency and general stress of the target cell or the host phagosome. When the bacteria or BTP lysis regulation system senses one or more of the above environmental conditions, bacterial or BTP lysis is triggered by one or more mechanisms including but not limited to antimicrobial proteins, bacteriophage lysins and autolysins expressed by the bacteria or BTP, either naturally or through modification, or through pore-forming proteins expressed by the bacteria or BTPs, either naturally or through modification, e.g., genetic modification, which break the phagosomes containing the bacteria or BTPs and liberate the siRNA-encoding plasmid or the one or more siRNAs.
The regulator of the lysis regulation system may be selected from the group that includes but is not limited to OmpR, ArcA, PhoP, PhoB, Fur, RstA, EvgA and RpoS. Preferable, lysis regulator sequences are recited in Table 6.









TABLE 6





Lysis Regulation




System

SEQ ID


Regulator Sequence
Sequence
NO:







OmpR regulator
ATGCAAGAGAACTACAAGATTC
29



TGGTGGTCGATGACGACATGCG




CCTGCGTGCGCTGCTGGAACGT




TATCTCACCGAACAAGGCTTCC




AGGTTCGAAGCGTCGCTAATGC




AGAACAGATGGATCGCCTGCTG




ACTCGTGAATCTTTCCATCTTA




TGGTACTGGATTTAATGTTACC




TGGTGAAGATGGCTTGTCGATT




TGCCGACGTCTTCGTAGTCAGA




GCAACCCGATGCCGATCATTAT




GGTGACGGCGAAAGGGGAAGAA




GTGGACCGTATCGTAGGCCTGG




AGATTGGCGCTGACGACTACAT




TCCAAAACCGTTTAACCCGCGT




GAACTGCTGGCCCGTATCCGTG




CGGTGCTGCGTCGTCAGGCGAA




CGAACTGCCAGGCGCACCGTCA




CAGGAAGAGGCGGTAATTGCTT




TCGGTAAGTTCAAACTTAACCT




CGGTACGCGCGAAATGTTCCGC




GAAGACGAGCCGATGCCGCTCA




CCAGCGGTGAGTTTGCGGTACT




GAAGGCACTGGTCAGCCATCCG




CGTGAGCCGCTCTCCCGCGATA




AGCTGATGAACCTTGCCCGTGG




TCGTGAATATTCCGCAATGGAA




CGCTCCATCGACGTGCAGATTT




CGCGTCTGCGCCGCATGGTGGA




AGAAGATCCAGCGCATCCGCGT




TACATTCAGACCGTCTGGGGTC




TGGGCTACGTCTTTGTACCGGA




CGGCTCTAAAGCATGA



 


PhoP regulator
ATGCGCGTACTGGTTGTTGAAG
30



ACAATGCGTTGTTACGTCACCA




CCTTAAAGTTCAGATTCAGGAT




GCTGGTCATCAGGTCGATGACG




CAGAAGATGCCAAAGAAGCCGA




TTATTATCTCAATGAACATATA




CCGGATATTGCGATTGTCGATC




TCGGATTGCCAGACGAGGACGG




TCTGTCACTGATTCGCCGCTGG




CGTAGCAACGATGTTTCACTGC




CGATTCTGGTATTAACCGCCCG




TGAAAGCTGGCAGGACAAAGTC




GAAGTATTAAGTGCCGGTGCTG




ATGATTATGTGACTAAACCGTT




TCATATTGAAGAGGTGATGGCG




CGAATGCAGGCATTAATGCGGC




GTAATAGCGGTCTGGCTTCACA




GGTCATTTCGCTCCCCCCGTTT




CAGGTTGATCTCTCTCGCCGTG




AATTATCTATTAATGACGAAGT




GATCAAACTGACCGCGTTCGAA




TACACTATTATGGAAACGTTGA




TACGCAATAATGGCAAAGTGGT




CAGCAAAGATTCGTTAATGCTC




CAACTCTATCCGGATGCGGAGC




TGCGGGAAAGCCATACCATTGA




TGTACTGATGGGACGTCTGCGC




AAAAAAATTCAGGCACAATATC




CCCAAGAAGTGATTACCACCGT




TCGCGGCCAGGGCTATCTGTTC




GAATTGCGCTGA









The promoter of the lysis regulation system may be selected from the group that includes but is not limited to ompF, ompC, fadB, phoPQ, mgtA, mgrB, psiB, phnD, Ptrp, sodA, sodB, sltA, sltB, asr, csgD, emrKY, yhiUV, acrAB, mdfA and tolC. Preferable, lysis regulation system promoter sequences are recited in Table 7.









TABLE 7





Lysis Regulation




System

SEQ ID 


Promoter Sequence
Sequence
NO:







ompF promoter
GATCATCCTGTTACGGAATATT
31



ACATTGCAACATTTACGCGCAA




AAACTAATCCGCATTCTTATTG




CGGATTAGTTTTTTCTTAGCTA




ATAGCACAATTTTCATACTATT




TTTTGGCATTCTGGATGTCTGA




AAGAAGATTTTGTGCCAGGTCG




ATAAAGTTTCCATCAGAAACAA




AATTTCCGTTTAGTTAATTTAA




ATATAAGGAAATCATATAAATA




GATTAAAATTGCTGTAAATATC




ATCACGTCTCTATGGAAATATG




ACGGTGTTCACAAAGTTCCTTA




AATTTTACTTTTGGTTACATAT




TTTTTCTTTTTGAAACCAAATC




TTTATCTTTGTAGCACTTTCAC




GGTAGCGAAACGTTAGTTTGAA




TGGAAAGATGCCTGCA



 


ompC promoter
TTTAAAAAAGTTCCGTAAAATT
32



CATATTTTGAAACATCTATGTA




GATAACTGTAACATCTTAAAAG




TTTTAGTATCATATTCGTGTTG




GATTATTCTGTATTTTTGCGGA




GAATGGACTTGCCGACTGGTTA




ATGAGGGTTAACCAGTAAGCAG




TGGCATAAAAAAGCAATAAAGG




CATATAACAGAGGGTTAATAAC



 


fadB promoter
AGTGATTCCATTTTTTACCCTT
33



CTGTTTTTTTGACCTTAAGTCT




CCGCATCTTAGCACATCGTTCA




TCCAGAGCGTGATTTCTGCCGA




GCGTGATCAGATCGGCATTTCT




TTAATCTTTTGTTTGCATATTT




TTAACACAAAATACACACTTCG




ACTCATCTGGTACGACCAGATC




ACCTTGCGGATTCAGGAGACTG




AC



 


phoPQ promoter
GAGCTATCACGATGGTTGATGA
34



GCTGAAATAAACCTCGTATCAG




TGCCGGATGGCGATGCTGTCCG




GCCTGCTTATTAAGATTATCCG




CTTTTTATTTTTTCACTTTACC




TCCCCTCCCCGCTGGTTTATTT




AATGTTTACCCCCATAACCACA




TAATCGCGTTACACTATTTTAA




TAATTAAGACAGGGAGAAATAA




AA



 


mgtA promoter
GCTTCAACACGCTCGCGGGTGA
35



GCTGGCTCACGCCGCTTTCGTT




ATTCAGCACCCGGGAAACTGTA




GATTTCCCCACGCCGCTTAAGC




GCGCGATATCTTTGATGGTCAG




CCGATTTTGCATCCTGTTGTCC




TGTAACGTGTTGTTTAATTATT




TGAGCCTAACGTTACCCGTGCA




TTCAGCAATGGGTAAAGTCTGG




TTTATCGTTGGTTTAGTTGTCA




GCAGGTATTATATCGCCA



 


Ptrp promoter
GAGCTGTTGACAATTAATCATC
36



GAACTAGTTAACTAGTACGCAA




GTTCACGTAAAAAGGGTATCTA




GAATTCT









The sensor of the lysis regulation system may be selected from the group that includes but is not limited to EnvZ, ArcB, PhoQ, PhoR, RstB and EvgS. Preferable, lysis regulation system sensor sequences are recited in Table 8.









TABLE 8





Lysis Regulation




System

SEQ ID


Sensor Sequence
Sequence
NO:







EnvZ sensor
ATGAGGCGATTGCGCTTCTCGC
37



CACGAAGTTCATTTGCCCGTAC




GTTATTGCTCATCGTCACCTTG




CTGTTCGCCAGCCTGGTGACGA




CTTATCTGGTGGTGCTGAACTT




CGCGATTTTGCCGAGCCTCCAG




CAGTTTAATAAAGTCCTCGCGT




ACGAAGTGCGTATGTTGATGAC




CGACAAACTGCAACTGGAGGAC




GGCACGCAGTTGGTTGTGCCTC




CCGCTTTCCGTCGGGAGATCTA




CCGTGAGCTGGGGATCTCTCTC




TACTCCAACGAGGCTGCCGAAG




AGGCAGGTCTGCGTTGGGCGCA




ACACTATGAATTCTTAAGCCAT




CAGATGGCGCAGCAACTGGGCG




GCCCGACGGAAGTGCGCGTTGA




GGTCAACAAAAGTTCGCCTGTC




GTCTGGCTGAAAACCTGGCTGT




CGCCCAATATCTGGGTACGCGT




GCCGCTGACCGAAATTCATCAG




GGCGATTTCTCTCCGCTGTTCC




GCTATACGCTGGCGATTATGCT




ATTGGCGATAGGCGGGGCGTGG




CTGTTTATTCGTATCCAGAACC




GACCGTTGGTCGATCTCGAACA




CGCAGCCTTGCAGGTTGGTAAA




GGGATTATTCCGCCGCCGCTGC




GTGAGTATGGCGCTTCGGAGGT




GCGTTCCGTTACCCGTGCCTTT




AACCATATGGCGGCTGGTGTTA




AGCAACTGGCGGATGACCGCAC




GCTGCTGATGGCGGGGGTAAGT




CACGACTTGCGCACGCCGCTGA




CGCGTATTCGCCTGGCGACTGA




GATGATGAGCGAGCAGGATGGC




TATCTGGCAGAATCGATCAATA




AAGATATCGAAGAGTGCAACGC




CATCATTGAGCAGTTTATCGAC




TACCTGCGCACCGGGCAGGAGA




TGCCGATGGAAATGGCGGATCT




TAATGCAGTACTCGGTGAGGTG




ATTGCTGCCGAAAGTGGCTATG




AGCGGGAAATTGAAACCGCGCT




TTACCCCGGCAGCATTGAAGTG




AAAATGCACCCGCTGTCGATCA




AACGCGCGGTGGCGAATATGGT




GGTCAACGCCGCCCGTTATGGC




AATGGCTGGATCAAAGTCAGCA




GCGGAACGGAGCCGAATCGCGC




CTGGTTCCAGGTGGAAGATGAC




GGTCCGGGAATTGCGCCGGAAC




AACGTAAGCACCTGTTCCAGCC




GTTTGTCCGCGGCGACAGTGCG




CGCACCATTAGCGGCACGGGAT




TAGGGCTGGCAATTGTGCAGCG




TATCGTGGATAACCATAACGGG




ATGCTGGAGCTTGGCACCAGCG




AGCGGGGCGGGCTTTCCATTCG




CGCCTGGCTGCCAGTGCCGGTA




ACGCGGGCGCAGGGCACGACAA




AAGAAGGGTAA



 


PhoQ sensor
ATGAAAAAATTACTGCGTCTTT
38



TTTTCCCGCTCTCGCTGCGGGT




ACGTTTTCTGTTGGCAACGGCA




GCGGTAGTACTGGTGCTTTCGC




TTGCCTACGGAATGGTCGCGCT




GATCGGTTATAGCGTCAGTTTC




GATAAAACTACGTTTCGGCTGT




TACGTGGCGAGAGCAATCTGTT




CTATACCCTTGCGAAGTGGGAA




AACAATAAGTTGCATGTCGAGT




TACCCGAAAATATCGACAAGCA




AAGCCCCACCATGACGCTAATT




TATGATGAGAACGGGCAGCTTT




TATGGGCGCAACGTGACGTGCC




CTGGCTGATGAAGATGATCCAG




CCTGACTGGCTGAAATCGAATG




GTTTTCATGAAATTGAAGCGGA




TGTTAACGATACCAGCCTCTTG




CTGAGTGGAGATCATTCGATAC




AGCAACAGTTGCAGGAAGTGCG




GGAAGATGATGACGAOGCGGAG




ATGACCCACTCGGTGGCAGTAA




ACGTCTACCCGGCAACATCGCG




GATGCCAAAATTAACCATTGTG




GTGGTGGATACCATTCCGGTGG




AGCTAAAAAGTTCCTATATGGT




CTGGAGCTGGTTTATCTATGTG




CTCTCAGCCAATCTGCTGTTAG




TGATCCCGCTGCTGTGGGTCGC




CGCCTGGTGGAGTTTACGCCCC




ATCGAAGCCCTGGCAAAAGAAG




TCCGCGAACTGGAAGAACATAA




CCGCGAATTGCTCAATCCAGCC




ACAACGCGAGAACTGACCAGTC




TGGTACGAAACCTGAACCGATT




GTTAAAAAGTGAACGCGAACGT




TACGACAAATACCGTACGACGC




TCACCGACCTGACCCATAGTCT




GAAAACGCCACTGGCGGTGCTG




CAAAGTACGCTGCGTTCTCTGC




GTAGTGAAAAGATGAGCGTCAG




TGATGCTGAGCCGGTAATGCTG




GAGCAAATCAGCCGCATTTCAC




AGCAAATTGGCTACTACCTGCA




TCGTGCCAGTATGCGCGGCGGG




ACATTGCTCAGCCGCGAGCTGC




ATCCGGTCGCCCCACTGCTGGA




CAATCTCACCTCAGCGCTGAAC




AAAGTGTATCAACGCAAAGGGG




TCAATATCTCTCTCGATATTTC




GCCAGAGATCAGCTTTGTCGGT




GAGCAGAACGATTTTGTCGAGG




TGATGGGCAACGTGCTGGATAA




TGCCTGTAAATATTGCCTCGAG




TTTGTCGAAATTTCTGCAAGGC




AAACCGACGAGCATCTCTATAT




TGTGGTCGAGGATGATGGCCCC




GGTATTCCATTAAGCAAGCGAG




AGGTCATTTTCGACCGTGGTCA




ACGGGTTGATACTTTACGCCCT




GGGCAAGGTGTAGGGCTGGCGG




TAGCCCGCGAAATCACCGAGCA




ATATGAGGGTAAAATCGTCGCC




GGAGAGAGCATGCTGGGCGGTG




CGCGGATGGAGGTGATTTTTGG




TCGCCAGCATTCTGCGCCGAAA




GATGAATAA









The lysis regulation system may comprise any combination of one or more of the above regulators, promoters and sensors.
In one example of this embodiment, the lysis regulation system comprises OmpR as the regulator, ompF as the promoter and EnvZ as the sensor and the stimulus is reduced osmolarity. In another example of this embodiment, the lysis regulation system comprises OmpR as the regulator, ompC as the promoter and EnvZ as the sensor and the stimulus is reduced osmolarity.
In another example of this embodiment, the lysis regulation system comprises the ArcA as the regulator, fad as the promoter and Arc B as the sensor and the stimulus is anaerobic conditions.
In another example of this embodiment, the lysis regulation system comprises PhoP as the regulator, phoPQ as the promoter and PhoQ as the sensor and the stimulus is reduced magnesium concentration. In another example of this embodiment, the lysis regulation system comprises PhoP as the regulator, mgtA as the promoter and PhoQ as the sensor and the stimulus is reduced magnesium concentration. In another example of this embodiment, the lysis regulation system comprises PhoP as the regulator, mgrB as the promoter and PhoQ as the sensor and the stimulus is reduced magnesium concentration.
In another example of this embodiment, the lysis regulation system comprises PhoB as the regulator, psiB as the promoter and PhoR as the sensor and the stimulus is reduced phosphate concentration. In another example of this embodiment, the lysis regulation system comprises PhoB as the regulator, phnD as the promoter and PhoR as the sensor and the stimulus is reduced phosphate concentration. In another example of this embodiment, the lysis regulation system comprises RstA as the regulator, asr as the promoter and RstB as the sensor. In another example of this embodiment, the lysis regulation system comprises RstA as the regulator, csgD as the promoter and RstB as the sensor.
In another example of this embodiment, the lysis regulation system comprises EvgA as the regulator, emrKY as the promoter and EvgS as the sensor. In another example of this embodiment, the lysis regulation system comprises EvgA as the regulator, yhiUV as the promoter and EvgS as the sensor. In another example of this embodiment, the lysis regulation system comprises EvgA as the regulator, acrAB as the promoter and EvgS as the sensor. In another example of this embodiment, the lysis regulation system comprises EvgA as the regulator, mdfA as the promoter and EvgS as the sensor. In another example of this embodiment, the lysis regulation system comprises EvgA as the regulator, tolC as the promoter and EvgS as the sensor.
In another example of this embodiment, the lysis regulation system comprises Fur as the regulator in combination with a promoter selected from the group comprising sodA, sodB, sltA or sltB.
The antimicrobial protein may be selected from the group that includes but is not limited to α-and β-defensins, protegrins, cathelicidins (e.g., indolicidin and bactenecins), granulysin, lysozyme, lactoferrin, azurocidin, elastase, bactericidal permeability inducing peptide (BPI), adrenomedullin, brevinin, histatins and hepcidin. Additional antimicrobial proteins are disclosed in the following, each of which is incorporated herein by reference in its entirety: Devine, D. A. et al., Current Pharmaceutical Design, 8, 703-714 (2002); Jack R. W., et al., Microbiological Reviews, 59 (2), 171-200 (June 1995).
Optionally, the antimicrobial protein is an α-defensin, β-defensin, or protegrin. Preferable, antimicrobial protein sequences are recited in Table 9.









TABLE 9





Antimicrobial Protein

SEQ ID


Sequence
Sequence
NO:







α-defensin-1 protein
CTATAGAAGACCTGGGACAGAG
39



GACTGCTGTCTGCCCTCTCTGG




TCACCCTGCCTAGCTAGAGGAT




CTGTGACCCCAGCCATGAGGAC




CCTCGCCATCCTTGCTGCCATT




CTCCTGGTGGCCCTGCAGGCCC




AGGCTGAGCCACTCCAGGCAAG




AGCTGATGAGGTTGCTGCAGCC




CCGGAGCAGATTGCAGCGGACA




TCCCAGAAGTGGTTGTTTCCCT




TGCATGGGACGAAAGCTTGGCT




CCAAAGCATCCAGGCTCAAGGA




AAAACATGGCCTGCTATTGCAG




AATACCAGCGTGCATTGCAGGA




GAACGTCGCTATGGAACCTGCA




TCTACCAGGGAAGACTCTGGGC




ATTCTGCTGCTGAGCTTGCAGA




AAAAGAAAAATGAGCTCAAAAT




TTGCTTTGAGAGCTACAGGGAA




TTGCTATTACTCCTGTACCTTC




TGCTCAATTTCCTTTCCTCATC




CCAAATAAATGCCTTGGTACAA




GAAAAG



 


α-defensin-3 protein
CCTTGCTATAGAAGACCTGGGA
40



CAGAGGACTGCTGTCTGCCCTC




TCTGGTCACCCTGCCTAGCTAG




AGGATCTGTGACCCCAGCCATG




AGGACCCTCGCCATCCTTGCTG




CCATTCTCCTGGTGGCCCTGCA




GGCCCAGGCTGAGCCACTCCAG




GCAAGAGCTGATGAGGTTGCTG




CAGCCCCGGAGCAGATTGCAGC




GGACATCCCAGAAGTGGTTGTT




TCCCTTGCATGGGACGAAAGCT




TGGCTCCAAAGCATCCAGGCTC




AAGGAAAAACATGGACTGCTAT




TGCAGAATACCAGCGTGCATTG




CAGGAGAACGTCGCTATGGAAC




CTGCATCTACCAGGGAAGACTC




TGGGCATTCTGCTGCTGAGCTT




GCAGAAAAAGAAAAATGAGCTC




AAAATTTGCTTTGAGAGCTACA




GGGAATTGCTATTACTCCTGTA




CCTTCTGCTCAATTTCCTTTCC




TCATCTCAAATAAATGCCTTGT




TAC



 


α-defensin-4 protein
GTCTGCCCTCTCTGCTCGCCCT
41



GCCTAGCTTGAGGATCTGTCAC




CCCAGCCATGAGGATTATCGCC




CTCCTCGCTGCTATTCTCTTGG




TAGCCCTCCAGGTCCGGGCAGG




CCCACTCCAGGCAAGAGGTGAT




GAGGCTCCAGGCCAGGAGCAGC




GTGGGCCAGAAGACCAGGACAT




ATCTATTTCCTTTGCATGGGAT




AAAAGCTCTGCTCTTCAGGTTT




CAGGCTCAACAAGGGGCATGGT




CTGCTCTTGCAGATTAGTATTC




TGCCGGCGAACAGAACTTCGTG




TTGGGAACTGCCTCATTGGTGG




TGTGAGTTTCACATACTGCTGC




ACGCGTGTCGATTAACGTTCTG




CTGTCCAAGAGAATGTCATGCT




GGGAACGCCATCATCGGTGGTG




TTAGCTTCACATGCTTCTGCAG




CTGAGCTTGCAGAATAGAGAAA




AATGAGCTCATAATTTGCTTTG




AGAGCTACAGGAAATGGTTGTT




TCTCCTATACTTTGTCCTTAAC




ATCTTTCTTGATCCTAAATATA




TATCTCGTAACAAG



 


α-defensin-5 protein
ATATCCACTCCTGCTCTCCCTC
42



CTGCAGGTGACCCCAGCCATGA




GGACCATCGCCATCCTTGCTGC




CATTCTCCTGGTGGCCCTGCAG




GCCCAGGCTGAGTCACTCCAGG




AAAGAGCTGATGAGGCTACAAC




CCAGAAGCAGTCTGGGGAAGAC




AACCAGGACCTTGCTATCTCCT




TTGCAGGAAATGGACTCTCTGC




TCTTAGAACCTCAGGTTCTCAG




GCAAGAGCCACCTGCTATTGCC




GAACCGGCCGTTGTGCTACCCG




TGAGTCCCTCTCCGGGGTGTGT




GAAATCAGTGGCCGCCTCTACA




GACTCTGCTGTCGCTGAGCTTC




CTAGATAGAAACCAAAGCAGTG




CAAGATTCAGTTCAAGGTCCTG




AAAAAAGAAAAACATTTTACTC




TGTGTACCTTGTGTCTTTCTAA




ATTTCTCTCTCCAAAATAAAGT




TCAAGCATT



 


α-defensin-6 protein
ACACATCTGCTCCTGCTCTCTC
43



TCCTCCAGCGACCCTAGCCATG




AGAACCCTCACCATCCTCACTG




CTGTTCTCCTCGTGGCCCTCCA




GGCCAAGGCTGAGCCACTCCAA




GCTGAGGATGATCCACTGCAGG




CAAAAGCTTATGAGGCTGATGC




CCAGGAGCAGCGTGGGGCAAAT




GACCAGGACTTTGCCGTCTCCT




TTGCAGAGGATGCAAGCTCAAG




TCTTAGAGCTTTGGGCTCAACA




AGGGCTTTCACTTGCCATTGCA




GAAGGTCCTGTTATTCAACAGA




ATATTCCTATGGGACCTGCACT




GTCATGGGTATTAACCACAGAT




TCTGCTGCCTCTGAGGGATGAG




AACAGAGAGAAATATATTCATA




ATTTACTTTATGACCTAGAAGG




AAACTGTCGTGTGTCCCATACA




TTGCCATCAACTTTGTTTCCTC




ATCTCAAATAAAGTCCTTTCAG




CAAAAAAAAAAAA



 


β-defensin-1 protein
TCCCTTCAGTTCCGTCGACGAG
44



GTTGTGCAATCCACCAGTCTTA




TAAATACAGTGACGCTCCAGCC




TCTGGAAGCCTCTGTCAGCTCA




GCCTCCAAAGGAGCCAGCGTCT




CCCCAGTTCCTGAAATCCTGGG




TGTTGCCTGCCAGTCGCCATGA




GAACTTCCTACCTTCTGCTGTT




TACTCTCTGCTTACTTTTGTCT




GAGATGGCCTCAGGTGGTAACT




TTCTCACAGGCCTTGGCCACAG




ATCTGATCATTACAATTGCGTC




AGCAGTGGAGGGCAATGTCTCT




ATTCTGCCTGCCCGATCTTTAC




CAAAATTCAAGGCACCTGTTAC




AGAGGGAAGGCCAAGTGCTGCA




AGTGAGCTGGGAGTGACCAGAA




GAAATGACGCAGAAGTGAAATG




AACTTTTTATAAGCATTCTTTT




AATAAAGGAAAATTGCTTTTGA




AGTATACCTCCTTTGGGCCAAA




AAAAAAAAAAAAAAAAAAAAAA



 


β-defensin-3 protein
TGAGTCTCAGCGTGGGGTGAAG
45



CCTAGCAGCTATGAGGATCCAT




TATCTTCTGTTTGCTTTGCTCT




TCCTGTTTTTGGTGCCTGTCCC




AGGTCATGGAGGAATCATAAAC




ACATTACAGAAATATTATTGCA




GAGTCAGAGGCGGCCGGTGTGC




TGTGCTCAGCTGCCTTCCAAAG




GAGGAACAGATCGGCAAGTGCT




CGACGCGTGGCCGAAAATGCTG




CCGAAGAAAGAAATAAAAACCC




TGAAACATGACGAGAGTGTTGT




AAAGTGTGGAAATGCCTTCTTA




AAGTTTATAAAAGTAAAATCAA




ATTACATTTTTTTTTCAAAAAA




AAAAAAA



 


β-defensin-4 protein
AGACTCAGCTCCTGGTGAAGCT
46



CCCAGCCATCAGCCATGAGGGT




CTTGTATCTCCTCTTCTCGTTC




CTCTTCATATTCCTGATGCCTC




TTCCAGGTGTTTTTGGTGGTAT




AGGCGATCCTGTTACCTGCCTT




AAGAGTGGAGCCATATGTCATC




CAGTCTTTTGCCCTAGAAGGTA




TAAACAAATTGGCACCTGTGGT




CTCCCTGGAACAAAATGCTGCA




AAAAGCCATGAGGAGGCCAAGA




AGCTGCTGTGGCTGATGCGGAT




TCAGAAAGGGCTCCCTCATCAG




AGACGTGCGACATGTAAACCAA




ATTAAACTATGGTGTCCAAAGA




TACGCA



 


protegrin-1 protein
ATGGAGACCCAGAGAGCCAGCC
47



TGTGCCTGGGGCGCTGGTCACT




GTGGCTTCTGCTGCTGGCACTC




GTGGTGCCCTCGGCCAGCGCCC




AGGCCCTCAGCTACAGGGAGGC




CGTGCTTCGTGCTGTGGATCGC




CTCAACGAGCAGTCCTCGGAAG




CTAATCTCTACCGCCTCCTGGA




GCTGGACCAGCCGCCCAAGGCC




GACGAGGACCCGGGCACCCCGA




AACCTGTGAGCTTCACGGTGAA




GGAGACTGTGTGTCCCAGGCCG




ACCCGGCAGCCCCCGGAGCTGT




GTGACTTCAAGGAGAACGGGCG




GGTGAAACAGTGTGTGGGGACA




GTCACCCTGGATCAGATCAAGG




ACCCGCTCGACATCACCTGCAA




TGAGGTTCAAGGTGTCAGGGGA




GGTCGCCTGTGCTATTGTAGGC




GTAGGTTCTGCGTCTGTGTCGG




ACGAGGATGACGGTTGCGACGG




CAGGCTTTCCCTCCCCCAATTT




TCCCGGGGCCAGGTTTCCGTCC




CCCAATTTTTCCGCCTCCACCT




TTCCGGCCCGCACCATTCGGTC




CACCAAGGTTCCCTGGTAGACG




GTGAAGGATTTGCAGGCAACTC




ACCCAGAAGGCCTTTCGGTACA




TTAAAATCCCAGCAAGGAGACC




TAAGCATCTGCTTTGCCCAGGC




CCGCATCTGTCAAATAAATTCT




TGTGAAACC



 


protegrin-3 protein
ATGGAGACCCAGAGAGCCAGCC
48



TGTGCCTGGGGCGCTGGTCACT




GTGGCTTCTGCTGCTGGCACTC




GTGGTGCCCTCGGCCAGCGCCC




AGGCCCTCAGCTACAGGGAGGC




CGTGCTTCGTGCTGTGGATCGC




CTCAACGAGCAGTCCTCGGAAG




CTAATCTCTACCGCCTCCTGGA




GCTGGACCAGCCGCCCAAGGCC




GACGAGGACCCGGGCACCCCGA




AACCTGTGAGCTTCACGGTGAA




GGAGACTGTGTGTCCCAGGCCG




ACCCGGCAGCCCCCGGAGCTGT




GTGACTTCAAGGAGAACGGGCG




GGTGAAACAGTGTGTGGGGACA




GTCACCCTGGATCAGATCAAGG




ACCCGCTCGACATCACCTGCAA




TGAGGTTCAAGGTGTCAGGGGA




GGTGGCCTGTGCTATTGTAGGC




GTAGGTTCTGCGTCTGTGTCGG




ACGAGGATGACGGTTGCGACGG




CAGGCTTTCCCTCCCCCAATTT




TCCCGGGGCCAGGTTTCCGTCC




CCCAATTTTTCCGCCTCCACCT




TTCCGGCCCGCACCATTCGGTC




CACCAAGGTTCCCTGGTAGACG




GTGAAGGATTTGCAGGCAACTC




ACCCAGAAGGCCTTTCGGTACA




TTAAAATCCCAGCAAGGAGACC




TAAGCATCTGCTTTGCCCAGGC




CCGCATCTGTCAAATAAATTCT




TGTGAAACC



 


protegrin-4 protein
ATGGAGACCCAGAGAGCCAGCC
49



TGTGCCTGGGGCGCTGGTCACT




GTGGCTTCTGCTGCTGGCACTC




GTGGTGCCCTCGGCCAGCGCCC




AGGCCCTCAGCTACAGGGAGGC




CGTGCTTCGTGCTGTGGATCGC




CTCAACGAGCAGTCCTCGGAAG




CTAATCTCTACCGCCTCCTGGA




GCTGGACCAGCCGCCCAAGGCC




GACGAGGACCCGGGCACCCCGA




AACCTGTGAGCTTCACGGTGAA




GGAGACTGTGTGTCCCAGGCCG




ACCCGGCAGCCCCCGGAGCTGT




GTGACTTCAAGGAGAACGGGCG




GGTGAAACAGTGTGTGGGGACA




GTCACCCTGGATCAGATCAAGG




ACCCGCTCGACATCACCTGCAA




TGAGGTTCAAGGTGTCAGGGGA




GGTCGCCTGTGCTATTGTAGGG




GTTGGATCTGCTTCTGTGTCGG




ACGAGGATGACGGTTGCGACGG




CAGGCTTTCCCTCCCCCAATTT




TCCCGGGGCCAGGTTTCCGTCC




CCCAATTTTTCCGCCTCCACCT




TTCCGGCCCGCACCATTCGGTC




CACCAAGGTTCCCTGGTAGACG




GTGAAGGATTTGCAGGCAACTC




ACCCAGAAGGCCTTTCGGCACA




TTAAAATCCCAGCAAGGAGACC




TAAGCATCTGCTTTGCCCAGGC




CCGCATCTGTCAAATAAATTCT




TGTGAAACC









The bacteriophase lysin may be selected from the group that includes but is not limited to holins and endolysins or lysins (e.g., lysozyme, amidase and transglycoslate). Additional lysins are disclosed in the following, each of which is incorporated herein by reference in its entirety: Kloos D.-U., et al., Journal of Bacteriology, 176 (23), 7352-7361 (December 1994); Jain V., et al., Infection and Immunity, 68 (2), 986-989 (February 2000); Srividhya K. V., et al., J. Biosci., 32, 979-990 (2007); Young R. V., Microbiological Reviews, 56 (3), 430-481 (September 1992).
The autolysin may be selected from the group that includes but is not limited to peptidoglycan hydrolases, amidases (e.g., N-acetylmuramyl-L-alanine amidases), transglycosylases, endopeptidases and glucosaminidases. Additional autolysins are disclosed in the following, each of which is incorporated herein by reference in its entirety: Heidrich C., et al., Molecular Microbiology, 41 (1), 167-178 (2001); Kitano K., et al., Journal of Bacteriology, 167 (3), 759-765 (September 1986); Lommatzsch J., et al., Journal of Bacteriology, 179 (17), 5465-5470 (September 1997); Oshida T., et al., PNAS, 92, 285-289 (January 1995); Lenz L. L., et al., PNAS, 100 (21), 12432-12437 (Oct. 14, 2003); Ramadurai L., et al., Journal of Bacteriology, 179 (11), 3625-3631 (June 1997); Kraft A. R., et al., Journal of Bacteriology, 180 (12), 3441-3447 (July 1998); Dijkstra A. J., et al., FEBS Letters, 366, 115-118 (1995); Huard C., et al., Microbiology, 149, 695-705 (2003).
In one aspect of the invention, the control exerted by the lysis regulation system may further be enhanced by bacterial or BTP strain-specific regulation. In one aspect of this embodiment, the strain-specific regulation is attenuation caused by deletion of a nutritional gene. The nutritional gene may be selected from the group that includes but is not limited to dapA, aroA and guaBA. In one example of this embodiment, dapA attenuation results in deficiency in the biosynthesis of lysine and peptidoglycan. In this particular embodiment, transcription of genes including but not limited to lysC may be activated by mechanisms such as transcriptional induction, antitermination and riboswitch. In another example of this embodiment, aroA attenuation results in deficiency in aromatic amino acids and derepression of one or more genes including but not limited to aroF, aroG and aroH by regulators such as TrpR and TyrR. In another example of this embodiment, guaBA attenuation results in derepression of one or more genes that are repressed by PurR.
In addition to the lysis regulation system and strain-specific regulation, the bacteria or BTP may further contain an inducible system that includes but is not limited to a Tet-on expression system to facilitate bacterial or BTP lysis at a time desired by the clinician. Upon administration of tetracycline, which activates the Tet-on promoter, the bacteria or BTP express a protein that triggers lysis of the bacteria or BTP. In one example of this embodiment, the protein expressed under the Tet-on expression system is selected from the group that includes but is not limited to defensins and protegrins.
The present invention also provides a lysis regulation system in combination with strain-specific attenuation (e.g., nutritional attenuation). As shown in FIG. 30, a global regulator can sense an extracellular condition and regulate transcription, starvation for specific nutrient such as an amino acid in vivo, in contrast to laboratory growth in the presence of excess of the nutrient and a positive or negative regulator in response to starvation. In the schematic shown in FIG. 31 there can be three cassettes, any of which may be place on either the bacterial chromosome or on a plasmid.
As described, the present invention provides a plasmid containing a lysis regulation system comprising OmpR as the regulator, ompF or ompC as the promoter and protegrin or β-defensin as the antimicrobial protein, in combination with a Tet-on expression system, which provides two levels of control of bacterial lysis. This embodiment is illustrated in FIG. 32.
In another aspect of the invention, the DNA insert comprises one or more of the following constructs, each of which contains an HPV target sequence, a hairpin sequence and BamHI and SaII restriction sites to facilitate incorporation into the hairpin RNA expression cassette of the TRIP plasmid as shown in Table 10.







TABLE 10







HPV Target Sequence Construct











BamHI
sense (19 bp)
loop
antisense (21 bp)
SaII





5′-GATCC
TAGGTATTTGAATTTGCAT
TTCAAGAGA
ATGCAAATTCAAATACCTTTT
G-3′ (SEQ ID NO: 50)


 


    3′-G
ATCCATAAACTTAAACGTA
AAGTTCTCT
TACGTTTAAGTTTATGGAAAA
CAGCT-5′ (SEQ ID NO: 51)









4. Cell and Gene Targets

The present invention also provides methods of using the various bacterium, BTP and vectors provided in the invention. For example, the present invention provides methods of delivering one or more siRNAs to mammalian cells. The methods include introducing at least one invasive bacterium, or at least one bacterial therapeutic particle (BTP), containing one or more siRNAs or one or more DNA molecules encoding one or more siRNAs to the mammalian cells.
The present invention also provides methods of regulating gene expression in mammalian cells. The method includes introducing at least one invasive bacterium, or at least one bacterial therapeutic particle (BTP), containing one or more siRNAs or one or more DNA molecules encoding one or more siRNAs to the mammalian cells, where the expressed siRNAs interfere with at least one mRNA of a gene of interest thereby regulating gene expression.
The invention provides a method for delivering RNA to any type of target cell. As used herein, the term “target cell” refers to a cell that can be invaded by a bacterium, i.e., a cell that has the necessary surface receptor for recognition by the bacterium.
Preferred target cells are eukaryotic cells. Even more preferred target cells are animal cells. “Animal cells” are defined as nucleated, non-chloroplast containing cells derived from or present in multicellular organisms whose taxanomic position lies within the kingdom animalia. The cells may be present in the intact animal, a primary cell culture, explant culture or a transformed cell line. The particular tissue source of the cells is not critical to the present invention.
The recipient animal cells employed in the present invention are not critical thereto and include cells present in or derived from all organisms within the kingdom animalia, such as those of the families mammalia, pisces, avian, reptilia.
Preferred animal cells are mammalian cells, such as humans, bovine, ovine, porcine, feline, canine, goat, equine, and primate cells. The most preferred mammalian cells are human cells. The cells can be in vivo, in vitro or ex vivo.
In some embodiments of the invention, the cell is a cervical epithelial cell, a rectal epithelial cell or a pharyngeal epithelial cell, macrophage, gastrointestinal epithelial cell, skin cell, melanocyte, keratinocyte, hair follicle, colon cancer cell, an ovarian cancer cell, a bladder cancer cell, a pharyngeal cancer cell, a rectal cancer cell, a prostate cancer cell, a breast cancer cell, a lung cancer cell, a renal cancer cell, a pancreatic cancer cell, or a hematologic cancer cell such as a lymphoma or leukemia cell. In one aspect of this embodiment, the colon cancer cell is an SW 480 cell. In another aspect of this embodiment, the pancreatic cancer cell is a CAPAN-1 cell.
In a preferred embodiment, the target cell is in a mucosal surface. Certain enteric pathogens, e.g., E. coli, Shigella, Listeria, and Salmonella, are naturally adapted for this application, as these organisms possess the ability to attach to and invade host mucosal surfaces (Kreig et al. supra). Therefore, in the present invention, such bacteria can deliver RNA molecules or RNA-encoding DNA to cells in the host mucosal compartment.
Although certain types of bacteria may have a certain tropism, i.e., preferred target cells, delivery of RNA or RNA-encoding DNA to a certain type of cell can be achieved by choosing a bacterium which has a tropism for the desired cell type or which is modified such as to be able to invade the desired cell type. Thus, e.g., a bacterium could be genetically engineered to mimic mucosal tissue tropism and invasive properties, as discussed above, to thereby allow said bacteria to invade mucosal tissue, and deliver RNA or RNA-encoding DNA to cells in those sites.
Bacteria can also be targeted to other types of cells. For example, bacteria can be targeted to erythrocytes of humans and primates by modifying bacteria to express on their surface either, or both of, the Plasmodium vivax reticulocyte binding proteins-1 and -2, which bind specifically to erythrocytes in humans and primates (Galinski et al. Cell, 69:1213-1226 (1992)). In another embodiment, bacteria are modified to have on their surface asialoorosomucoid, which is a ligand for the asilogycoprotein receptor on hepatocytes (Wu et al. J. Biol. Chem., 263:14621-14624 (1988)). In yet another embodiment, bacteria are coated with insulin-poly-L-lysine, which has been shown to target plasmid uptake to cells with an insulin receptor (Rosenkranz et al. Expt. Cell Res., 199:323-329 (1992)). Also within the scope of the invention are bacteria modified to have on their surface p60 of Listeria monocytogenes, which allows for tropism for hepatocytes (Hess et al. Infect. Immun., 63:2047-2053 (1995)), or a 60 kD surface protein from Trypanosoma cruzi which causes specific binding to the mammalian extra-cellular matrix by binding to heparin, heparin sulfate and collagen (Ortega-Barria et al. Cell, 67:411-421 (1991)).
Yet in another embodiment, a cell can be modified to become a target cell of a bacterium for delivery of RNA. Accordingly, a cell can be modified to express a surface antigen that is recognized by a bacterium for its entry into the cell, i.e., a receptor of an invasion factor. The cell can be modified either by introducing into the cell a nucleic acid encoding a receptor of an invasion factor, such that the surface antigen is expressed in the desired conditions. Alternatively, the cell can be coated with a receptor of an invasion factor. Receptors of invasion factors include proteins belonging to the integrin receptor superfamily. A list of the type of integrin receptors recognized by various bacteria and other microorganisms can be found, e.g., in Isberg and Tran Van Nhieu (1994) Ann. Rev. Genet. 27:395. Nucleotide sequences for the integrin subunits can be found, e.g., in GenBank, publicly available on the internet.
As set forth above, yet other target cells include fish, avian, and reptilian cells. Examples of bacteria that are naturally invasive for fish, avian, and reptilian cells are set forth below.
Examples of bacteria that can naturally access the cytoplasm of fish cells include, but are not limited to, Aeromonas salminocida (ATCC No. 33658) and Aeromonas schuberii (ATCC No. 43700). Attenuated bacteria are preferably used in the invention, and include A. salmonicidia vapA (Gustafson et al. J. Mol. Biol., 237:452-463 (1994)) or A. salmonicidia aromatic-dependent mutant (Vaughan et al. Infect. Immun., 61:2172-2181 (1993)).
Examples of bacteria that can naturally access the cytoplasm of avian cells include, but are not restricted to, Salmonella galinarum (ATCC No. 9184), Salmonella enteriditis (ATCC No. 4931) and Salmonella typhimurium (ATCC No. 6994). Attenuated bacteria are preferred to the invention and include attenuated Salmonella strains such as S. galinarum cya crp mutant (Curtiss et al. (1987) supra) or S. enteritidis aroA aromatic-dependent mutant CVL30 (Cooper et al. Infect. Immun., 62:4739-4746 (1994)).
Examples of bacteria that can naturally access the cytoplasm of reptilian cells include, but are not restricted to, Salmonella typhimurium (ATCC No. 6994). Attenuated bacteria are preferable to the invention and include, attenuated strains such as S. typhimuirum aromatic-dependent mutant (Hormaeche et al. supra).
The invention also provides for delivery of RNA to other eukaryotic cells, e.g., plant cells, so long as there are microorganisms that are capable of invading such cells, either naturally or after having been modified to become invasive. Examples of microorganisms which can invade plant cells include Agrobacterium tumerfacium, which uses a pilus-like structure which binds to the plant cell via specific receptors, and then through a process that resembles bacterial conjugation, delivers at least some of its content to the plant cell.
Set forth below are examples of cell lines to which RNA can be delivered according to the method of this invention.
Examples of human cell lines include but are not limited to ATCC Nos. CCL 62, CCL 159, HTB 151, HTB 22, CCL 2, CRL 1634, CRL 8155, HTB 61, and HTB104.
Examples of bovine cell lines include ATCC Nos. CRL 6021, CRL 1733, CRL 6033, CRL 6023, CCL 44 and CRL 1390.
Examples of ovine cells lines include ATCC Nos. CRL 6540, CRL 6538, CRL 6548 and CRL 6546.
Examples of porcine cell lines include ATCC Nos. CL 184, CRL 6492, and CRL 1746.
Examples of feline cell lines include CRL 6077, CRL 6113, CRL 6140, CRL 6164, CCL 94, CCL 150, CRL 6075 and CRL 6123.
Examples of buffalo cell lines include CCL 40 and CRL 6072.
Examples of canine cells include ATCC Nos. CRL 6213, CCL 34, CRL 6202, CRL 6225, CRL 6215, CRL 6203 and CRL 6575.
Examples of goat derived cell lines include ATCC No. CCL 73 and ATCC No. CRL 6270.
Examples of horse derived cell lines include ATCC Nos. CCL 57 and CRL 6583.
Examples of deer cell lines include ATCC Nos. CRL 6193-6196.
Examples of primate derived cell lines include those from chimpanzee's such as ATCC Nos. CRL 6312, CRL 6304, and CRL 1868; monkey cell lines such as ATCC Nos. CRL 1576, CCL 26, and CCL 161; orangautan cell line ATCC No. CRL 1850; and gorilla cell line ATCC No. CRL 1854.
The invention also provides methods of regulating the expression of one or more genes. Preferably, regulating the expression of one or more genes means decreasing or lessening the expression of the gene and/or decreasing or lessening the activity of the gene and its corresponding gene product.
In one embodiment, the expressed siRNAs direct the multienzyme complex RISC (RNA-induced silencing complex) of the cell to interact with the mRNAs to be regulated. This complex degrades or sequesters the mRNA. This causes the expression of the gene to be decreased or inhibited.
In some embodiments, the gene is an animal gene. Preferred animal genes are mammalian genes, such as humans, bovine, ovine, porcine, feline, canine, goat, equine, and primate genes. The most preferred mammalian genes are human cells.
The gene to be regulated can be a viral gene, anti-inflammatory gene, obesity gene or automimmune disease or disorder gene. In some embodiments, more than one gene can be regulated from a single plasmid or vector.
In preferred embodiments, the gene can be, but is not limited to, ras, β-catenin, one or more HPV oncogenes, APC, HER-2, MDR-1, MRP-2, FATP4, SGLUT-1, GLUT-2, GLUT-5, apobec-1, MTP, IL-6, IL-6R, IL-7, IL-12, IL-13, IL-13 Ra-1, IL-18, p38/JNK MAP kinase, p65/NF-κB, CCL20 (MIP-3α), Claudin-2, Chitinase 3-like 1, apoA-IV, MHC class I and MHC class II. In one aspect of this embodiment, the ras is k-Ras. In another aspect of this embodiment, the HPV oncogene is E6 or E7.
Preferable β-catenin target gene sequences are recited in Table 11. The sequences in Table 11 are cross-species target sequences as they are capable of silencing the beta-catenin gene (CTNNB1) in human, mouse, rat, dog and monkey.










TABLE 11







β-catenin target gene sequences
SEQ ID NO:









AGCCAATGGCTTGGAATGAGA
52



 



ATCAGCTGGCCTGGTTTGATA
53



 



CTGTGAACTTGCTCAGGACAA
54



 



AGCAATCAGCTGGCCTGGTTT
55



 



CCTCTGTGAACTTGCTCAGGA
56



 



TTCCGAATGTCTGAGGACAAG
57



 



CCAATGGCTTGGAATGAGACT
58



 



GGTGCTGACTATCCAGTTGAT
59



 



CAATCAGCTGGCCTGGTTTGA
60



 



CACCCTGGTGCTGACTATCCA
61



 



CACCACCCTGGTGCTGACTAT
62



 



TGCTTTATTCTCCCATTGAAA
63



 



CTGGTGCTGACTATCCAGTTG
64



 



TCTGTGCTCTTCGTCATCTGA
65



 



TGCCATCTGTGCTCTTCGTCA
66



 



TGGTGCTGACTATCCAGTTGA
67



 



CCTGGTGCTGACTATCCAGTT
68



 



ACCCTGGTGCTGACTATCCAG
69



 



GAGCCTGCCATCTGTGCTCTT
70



 



CTGGTTTGATACTGACCTGTA
71



 



TGGTTTGATACTGACCTGTAA
72



 



TCGAGGAGTAACAATACAAAT
73



 



ACCATGCAGAATACAAATGAT
74



 



AGGAGTAACAATACAAATGGA
75



 



GTCGAGGAGTAACAATACAAA
76



 



TTGTTGTAACCTGCTGTGATA
77



 



GAGTAATGGTGTAGAACACTA
78



 



AGTAATGGTGTAGAACACTAA
79



 



CACACTAACCAAGCTGAGTTT
80



 



TTTGGTCGAGGAGTAACAATA
81



 



TACCATTCCATTGTTTGTGCA
82



 



TAGGGTAAATCAGTAAGAGGT
83



 



CTAACCAAGCTGAGTTTCCTA
84



 



TGGTCGAGGAGTAACAATACA
85



 



CTGGCCTGGTTTGATACTGAC
86



 



TAACCTCACTTGCAATAATTA
87



 



ATCCCACTGGCCTCTGATAAA
88



 



GACCACAAGCAGAGTGCTGAA
89



 



CACAAGCAGAGTGCTGAAGGT
90



 



CTAACCTCACTTGCAATAATT
91



 



AGCTGATATTGATGGACAG
92










Preferable HPV target gene sequences are recited in Table 12. The sequences in Table 12 are target sequences as they are capable of silencing the HPV E6 oncogene.










TABLE 12







HPV target gene sequences
SEQ ID NO:



















CGGTGCCAGAAACCGTTGAATCC
93



 



CACTGCAAGACATAGAAATAACC
94



 



AGGTGCCTGCGGTGCCAGAAACC
95



 



GCGGTGCCAGAAACCGTTGAATC
96



 



TCACTGCAAGACATAGAAATAAC
97



 



CCCATGCTGCATGCCATAAATGT
98



 



ATGCTGCATGCCATAAATGTATA
99



 



GTGGTGTATAGAGACAGTATACC
100



 



GCGCGCTTTGAGGATCCAACACG
101



 



CTGCGGTGCCAGAAACCGTTGAA
102



 



CCCCATGCTGCATGCCATAAATG
103



 



ACCCCATGCTGCATGCCATAAAT
104



 



AACACTGGGTTATACAATTTATT
105



 



ACGACGCAGAGAAACACAAGTAT
106



 



AAGGTGCCTGCGGTGCCAGAAAC
107



 



GGTGCCTGCGGTGCCAGAAACCG
108



 



CATGCTGCATGCCATAAATGTAT
109



 



GACGCAGAGAAACACAAGTATAA
110



 



TTCACTGCAAGACATAGAAATAA
111



 



GGTGCCAGAAACCGTTGAATCCA
112



 



TGGCGCGCTTTGAGGATCCAACA
113



 



TGTGGTGTATAGAGACAGTATAC
114



 



GTGCCTGCGGTGCCAGAAACCGT
115



 



CTGCATGCCATAAATGTATAGAT
116



 



GACTCCAACGACGCAGAGAAACA
117



 



CTGGGCACTATAGAGGCCAGTGC
118



 



TGCTGCATGCCATAAATGTATAG
119



 



GTGCCAGAAACCGTTGAATCCAG
120



 



TTACAGAGGTATTTGAATTTGCA
121



 



GAGGCCAGTGCCATTCGTGCTGC
122










Additional preferable HPV target gene sequences are recited in Table 13. The sequences in Table 13 are target sequences as they are capable of silencing the HPV E7 oncogene.










TABLE 13







HPV target gene sequences
SEQ ID NO:









ATTCCGGTTGACCTTCTATGTCA
123



 



GATGGAGTTAATCATCAACATTT
124



 



AAGCCAGAATTGAGCTAGTAGTA
125



 



CATGGACCTAAGGCAACATTGCA
126



 



AACCACAACGTCACACAATGTTG
127



 



ATGGACCTAAGGCAACATTGCAA
128



 



TAAGCGACTCAGAGGAAGAAAAC
129



 



GAAGCCAGAATTGAGCTAGTAGT
130



 



GAGCCGAACCACAACGTCACACA
131



 



ACGTCACACAATGTTGTGTATGT
132



 



GAACCACAACGTCACACAATGTT
133



 



AGGCAACATTGCAAGACATTGTA
134



 



AAGACATTGTATTGCATTTAGAG
135



 



TAAGGCAACATTGCAAGACATTG
136



 



CCAGCCCGACGAGCCGAACCACA
137



 



AAGCTCAGCAGACGACCTTCGAG
138



 



GCCCGACGAGCCGAACCACAACG
139



 



TTCCGGTTGACCTTCTATGTCAC
140



 



TGCATGGACCTAAGGCAACATTG
141



 



TTCCAGCAGCTGTTTCTGAACAC
142



 



AACACCCTGTCCTTTGTGTGTCC
143



 



CTTCTATGTCACGAGCAATTAAG
144



 



ACGAGCCGAACCACAACGTCACA
145



 



TTGAGCTAGTAGTAGAAAGCTCA
146



 



CAGCAGACGACCTTCGAGCATTC
147



 



AGCCAGAATTGAGCTAGTAGTAG
148



 



GTCACACAATGTTGTGTATGTGT
149



 



CCGACGAGCCGAACCACAACGTC
150



 



AATTCCGGTTGACCTTCTATGTC
151



 



ATTCCAGCAGCTGTTTCTGAACA
152










Additional preferable HPV target gene sequences are recited in Table 14. The sequences in Table 14 are target sequences shared by both HPV E6 and E7.










TABLE 14







HPV target gene sequences
SEQ ID NO:









TAGGTATTTGAATTTGCAT
153



 



GAGGTATTTGAATTTGCAT
154










A preferable MDR-1 target gene sequence is recited in Table 15. The sequence in Table 15 is capable of silencing the MDR-1 gene in human.










TABLE 15







MDR-1 target gene sequences
SEQ ID NO:









ATGTTGTCTGGACAAGCACT
155










A preferable k-Ras target gene sequence is recited in Table 16. The sequence in Table 16 is capable of silencing the k-Ras gene in human.










TABLE 16







k-Ras target gene sequences
SEQ ID NO:









GTTGGAGCTGTTGGCGTAG
156










Preferable IL-6R target gene sequence are recited in Table 17. The sequences in Table 17 are capable of silencing IL-6R in human.










TABLE 17







IL-6R target gene sequences
SEQ ID NO:









CTCCTGGAACTCATCTTTCTA
157



 



GCTCTCCTGCTTCCGGAAGAG
158



 



CTCCACGACTCTGGAAACTAT
159



 



CAGAAGTTCTCCTGCCAGTTA
160



 



CCGGAAGACAATGCCACTGTT
161



 



CTGAACGGTCAAAGACATTCA
162



 



CACAACATGGATGGTCAAGGA
163



 



ATGCAGGCACTTACTACTAAT
164



 



ATCGGGCTGAACGGTCAAAGA
165



 



AGCTCTCCTGCTTCCGGAAGA
166



 



CAGCTCTCCTGCTTCCGGAAG
167



 



CAGGCACTTACTACTAATAAA
168



 



CACTTGCTGGTGGATGTTCCC
169



 



AACGGTCAAAGACATTCACAA
170



 



TGCACAAGCTGCACCCTCAGG
171










Additional referable IL-6R target gene sequences are recited in Table 18. The sequences in Table 18 are capable of silencing the IL-6R gene in mouse.










TABLE 18







IL-6R target gene sequences
SEQ ID NO:









ATCCTGGAGGGTGACAAAGTA
172



 



TGGGTCTGACAATACCGTAAA
173



 



AACGAAGCGTTTCACAGCTTA
174



 



CCGCTGTTTCCTATAACAGAA
175



 



ACGAAGCGTTTCACAGCTTAA
176



 



CTGCTGTGAAAGGGAAATTTA
177



 



AACCTTGTGGTATCAGCCATA
178



 



CACAGTGTGGTGCTTAGATTA
179



 



CAGCTTCGATACCGACCTGTA
180



 



CAGTGTGGTGCTTAGATTAAA
181



 



CCCGGCAGGAATCCTCTGGAA
182



 



CCCGCTGTTTCCTATAACAGA
183



 



AACCACGAGGATCAGTACGAA
184



 



ACCTGCCGTCTTACTGAACTA
185



 



ACCACGAGGATCAGTACGAAA
186



 



ACAGCTTGTGATGACTGAATA
187



 



AGGATCAGTACGAAAGTTCTA
188



 



AACCCGCTGTTTCCTATAACA
189



 



CAGTACGAAAGTTCTACAGAA
190



 



TACGCGAGTGACAATTTCTCA
191



 



ACGAAAGTTCTACAGAAGCAA
192



 



CAGGCACTTACTACTAATAAA
193



 



CACTTGCTGGTGGATGTTCCC
194



 



AACGGTCAAAGACATTCACAA
195



 



TGCACAAGCTGCACCCTCAGG
196










Preferable IL-7 target gene sequences are recited in Table 19. The sequences in Table 19 are capable of silencing the IL-7 gene in human.










TABLE 19







IL-7 target gene sequences
SEQ ID NO:









TAAGAGAGTCATAAACCTTAA
197



 



AACAAGGTCCAAGATACCTAA
198



 



AAGATTGAACCTGCAGACCAA
199



 



AAGAGATTTCAAGAGATTTAA
200



 



AAGCGCAAAGTAGAAACTGAA
201



 



TAGCATCATCTGATTGTGATA
202



 



TAAGATAATAATATATGTTTA
203



 



ATGGTCAGCATCGATCAATTA
204



 



TTGCCTGAATAATGAATTTAA
205



 



ATCTGTGATGCTAATAAGGAA
206



 



AACAAACTATTTCTTATATAT
207



 



AACATTTATCAATCAGTATAA
208



 



ATCAATCAGTATAATTCTGTA
209



 



AAGGTATCAGTTGCAATAATA
210










Additional preferable IL-7 target gene sequences are recited in Table 20. The sequences in Table 20 are capable of silencing the IL-7 gene in mouse.










TABLE 20







IL-7 target gene sequences
SEQ ID NO:









CGGATCCTACGGAAGTTATGG
211



 



GACCATGTTCCATGTTTCTTT
212



 



AACCTAAATGACCTTTATTAA
213



 



CAGGAGACTAGGACCCTATAA
214



 



TAGGGTCTTATTCGTATCTAA
215



 



ATGAGCCAATATGCTTAATTA
216



 



GCCAATATGCTTAATTAGAAA
217



 



CAGCATCGATGAATTGGACAA
218



 



TTGCCTGAATAATGAATTTAA
219



 



CTGATAGTAATTGCCCGAATA
220



 



AAGGGTTTGCTTGTACTGAAT
221



 



AACATGTATGTGATGATACAA
222



 



TTGCAACATGTAATAATTTAA
223



 



AAGAGACTACTGAGAGAAATA
224



 



AAGAATCTACTGGTTCATATA
225



 



TGCCGTCAGCATATACATATA
226



 



AGGGCTCACGGTGATGGATAA
227










Additional preferable IL-7 target gene sequences are recited in Table 21. The sequences in Table 21 are cross species sequences as they are capable of silencing the IL-7 gene in human and mouse.










TABLE 21







IL-7 target gene sequences
SEQ ID NO:









CGCCTCCCGCAGACCATGTTC
228



 



TCCGTGCTGCTCGCAAGTTGA
229



 



GCCTCCCGCAGACCATGTTCC
230



 



CCTCCCGCAGACCATGTTCCA
231



 



CTCCCGCAGACCATGTTCCAT
232



 



TCCCGCAGACCATGTTCCATG
233



 



CCCGCAGACCATGTTCCATGT
234



 



CCGCAGACCATGTTCCATGTT
235



 



CGCAGACCATGTTCCATGTTT
236



 



GCAGACCATGTTCCATGTTTC
237



 



CAGACCATGTTCCATGTTTCT
238



 



AGACCATGTTCCATGTTTCTT
239










Preferable IL-13Ra-1 target gene sequences are recited in Table 22. The sequences in Table 22 are capable of silencing the IL-13Ra-1 gene in human.










TABLE 22







IL-13Ra-1 target gene sequences
SEQ ID NO:









AACCTGATCCTCCACATATTA
240



 



CCTGATCCTCCACATATTAAA
241



 



AGAAATGTTTGGAGACCAGAA
242



 



CAAATAATGGTCAAGGATAAT
243



 



TTCCTGATCCTGGCAAGATTT
244



 



TAAAGAAATGTTTGGAGACCA
245



 



ATGTTTGGAGACCAGAATGAT
246



 



CTCCAATTCCTGATCCTGGCA
247










Additional preferable IL-13Ra-1 target gene sequences are recited in Table 23. The sequences in Table 23 are capable of silencing the IL-13Ra-1 gene in mouse.










TABLE 23







IL-13Ra-1 target gene sequences
SEQ ID NO:









CAAGAAGACTCTAATGATGTA
248



 



CACAGTCAGAGTAAGAGTCAA
249



 



ACCCAGGGTATCATAGTTCTA
250



 



CTGCTTTGAAATTTCCAGAAA
251



 



ATCATAGTTCTAAGAATGAAA
252



 



AAGGCTTAAGATCATTATATT
253



 



AACTACTTATAAGAAAGTAAA
254



 



CACAGAACATCTAGCAAACAA
255



 



CTCGTTCTTGTTCAATCCTAA
256



 



AACTTGTAGGTTCACATATTA
257



 



AACCATTTCTGCAAATTTAAA
258



 



CTCAGTGTAGTGCCAATGAAA
259



 



CAGGCCTTAGGGACTCATAAA
260



 



AAGTATGACATCTATGAGAAA
261



 



GTGGAGGTCAATAATACTCAA
262



 



CAGAGTATAGGTAAGGAGCAA
263










A preferable IL-18 target gene sequence is recited in Table 24. The sequence in Table 24 is capable of silencing the IL-18 gene in human.










TABLE 24







IL-18 target gene sequences
SEQ ID NO:









TTGAATGACCAAGTTCTCTTC
264










Additional preferable IL-18 target gene sequences are recited in Table 25. The sequences in Table 25 are capable of silencing the IL-18 gene in mouse.










TABLE 25







IL-18 target gene sequences
SEQ ID NO:









CTCTCTGTGAAGGATAGTAAA
265



 



CCGCAGTAATACGGAATATAA
266



 



CAAGGAAATGATGTTTATTGA
267



 



CAGACTGATAATATACATGTA
268



 



TTGGCCGACTTCACTGTACAA
269



 



CCAGACCAGACTGATAATATA
270



 



AAGATGGAGTTTGAATCTTCA
271



 



ACGCTTTACTTTATACCTGAA
272



 



TACAACCGCAGTAATACGGAA
273



 



CTGCATGATTTATAGAGTAAA
274



 



CCCGAGGCTGCATGATTTATA
275



 



CACGCTTTACTTTATACCTGA
276



 



CGCCTGTATTTCCATAACAGA
277



 



CGCAGTAATACGGAATATAAA
278



 



TACATGTACAAAGACAGTGAA
279



 



CAGGCCTGACATCTTCTGCAA
280



 



TTCGAGGATATGACTGATATT
281



 



CTGTATTTCCATAACAGAATA
282



 



GAGGATATGACTGATATTGAT
283



 



CAAGTTCTCTTCGTTGACAAA
284



 



CACTAACTTACATCAAAGTTA
285



 



ACCGCAGTAATACGGAATATA
286



 



CTCTCACTAACTTACATCAAA
287










Preferable CCL20 target gene sequences are recited in Table 26. The sequences in Table 26 are capable of silencing the CCL20 gene in human.










TABLE 26







CCL20 target gene sequences
SEQ ID NO:









ATCATCTTTCACACAAAGAAA
288



 



AACAGACTTGGGTGAAATATA
289



 



ATGGAATTGGACATAGCCCAA
290



 



GAGGGTTTAGTGCTTATCTAA
291



 



CTCACTGGACTTGTCCAATTA
292



 



ATCATAGTTTGCTTTGTTTAA
293



 



TTGTTTAAGCATCACATTAAA
294



 



AAGCATCACATTAAAGTTAAA
295



 



CCCAAAGAACTGGGTACTCAA
296



 



CACATTAAAGTTAAACTGTAT
297



 



CAGATCTGTTCTTTGAGCTAA
298



 



TTGGTTTAGTGCAAAGTATAA
299



 



CAGACCGTATTCTTCATCCTA
300



 



AACATTAATAAGACAAATATT
301



 



GACCGTATTCTTCATCCTAAA
302










Additional referable CCL20 target gene sequences are recited in Table 27. The sequences in Table 27 are capable of silencing the CCL20 gene in mouse.










TABLE 27







CCL20 target gene sequences
SEQ ID NO:









AAGCTTGTGACATTAATGCTA
303



 



CAATAAGCTATTGTAAAGATA
304



 



ATCATCTTTCACACGAAGAAA
305



 



AGCTATTGTAAAGATATTTAA
306



 



CAGCCTAAGAGTCAAGAAGAT
307



 



CCCAGTGGACTTGTCAATGGA
308



 



ATGAAGTTGATTCATATTGCA
309



 



AAGTTGATTCATATTGCATCA
310



 



TCACATTAGAGTTAAGTTGTA
311



 



CACATTAGAGTTAAGTTGTAT
312



 



TATGTTATTTATAGATCTGAA
313



 



ATGTTTAGCTATTTAATGTTA
314



 



TTAGTGGAAGGATTAATATTA
315



 



ACCCAGCACTGAGTACATCAA
316



 



TATGTTTAAGGGAATAGTTTA
317










Additional preferable CCL20 target gene sequences are recited in Table 28. The sequences in Table 28 are cross-species target sequences as they are capable of silencing the CCL20 gene in human and mouse.










TABLE 28







CCL20 target gene sequences
SEQ ID NO:









ATGAAGTTGATTCATATTGCA
318



 



TGAAGTTGATTCATATTGCAT
319



 



GAAGTTGATTCATATTGCATC
320



 



AAGTTGATTCATATTGCATCA
321



 



AGTTGATTCATATTGCATCAT
322



 



GTTGATTCATATTGCATCATA
323



 



TTGATTCATATTGCATCATAG
324



 



TGATTCATATTGCATCATAGT
325



 



TCAATGCTATCATCTTTCACA
326



 



CAATGCTATCATCTTTCACAC
327



 



TAATGAAGTTGATTCATATTG
328



 



AATGAAGTTGATTCATATTGC
329










Preferable CCL20 target gene sequences are recited in Table 29. The sequences in Table 29 are capable of silencing the CCL20 gene in human.










TABLE 29







Claudin-2 target gene sequences
SEQ ID NO:









AGCATGAAATTTGAGATTGGA
330



 



TACAGAGCCTCTGAAAGACCA
331



 



CACTACAGAGCCTCTGAAAGA
332



 



CTGACAGCATGAAATTTGAGA
333



 



ATCTCTGTGGTGGGCATGAGA
334



 



CATGAAATTTGAGATTGGAGA
335



 



TCTGGCTGAGGTTGGCTCTTA
336



 



GTGGGCTACATCCTAGGCCTT
337










Additional preferable CCL20 target gene sequences are recited in Table 30. The sequences in Table 30 are capable of silencing the CCL20 gene in mouse.










TABLE 30







Claudin-2 target gene sequences
SEQ ID NO:









CAGCTTCCTGCTAAACCACAA
338



 



CAAGAGTGAGTTCAACTCATA
339



 



CTGGTTCCTGACAGCATGAAA
340



 



TGGCTGGGACTATATATATAA
341



 



GAGGGCAATTGCTATATCTTA
342



 



CAGCAGCCAAACGACAAGCAA
343



 



CAAGGGTTTCCTTAAGGACAA
344



 



CAGATACTTGTAAGGAGGAAA
345



 



AAGAAATGGATTAGTCAGTAA
346



 



AAGGAAAGCACAAGAAGCCAA
347



 



CTGGCTGAGGTTGGCTCTTAA
348



 



AACCTGGGATCTAAAGAAACA
349



 



AAGGGCTTGGGTATCAAAGAA
350



 



CAGGCTCCGAAGATACTTCTA
351



 



CCCAATATATAAATTGCCTAA
352



 



CTGACCCAGCTTCCTGCTAAA
353










Preferable Chitinase-3 target gene sequences are recited in Table 31. The sequences in Table 31 are capable of silencing the Chitinase-3 gene in human.








TABLE 31





Chitinase-3 target gene sequences
SEQ ID NO:







ACCCACATCATCTACAGCTTT
354


 


CATCATCTACAGCTTTGCCAA
355


 


CAGCTGGTCCCAGTACCGGGA
356


 


CACCAAGGAGGCAGGGACCCT
357


 


CCGGTTCACCAAGGAGGCAGG
358


 


AGCTGGTCCCAGTACCGGGAA
359


 


CAGGCCGGTTCACCAAGGAGG
360


 


GGCCGGTTCACCAAGGAGGCA
361









Additional preferable Chitinase-3 target gene sequences are recited in Table 32. The sequences in Table 32 are capable of silencing the Chitinase-3 gene in mouse.








TABLE 32





Chitinase-3 target gene sequences
SEQ ID NO:







TAGGTTTGACAGATACAGCAA
362


 


AACCCTGTTAAGGAATGCAAA
363


 


ATCAAGTAGGCAAATATCTTA
364


 


CGCAGCTTTGTCAGCAGGAAA
365


 


TTGGATCAAGTAGGCAAATAT
366


 


TTGAGGGACCATACTAATTAT
367


 


GAGGACAAGGAGAGTGTCAAA
368


 


TGCGTACAAGCTGGTCTGCTA
369


 


CAGGAGTTTAATCTCTTGCAA
370


 


ATCAAGGAACTGAATGCGGAA
371


 


CACCCTGATCAAGGAACTGAA
372


 


CACTTGGATCAAGTAGGCAAA
373


 


CAGGATTGAGGGACCATACTA
374


 


AACTATGACAAGCTGAATAAA
375


 


ATGCAAATTCTCAGACTCTAA
376


 


ATCCTTCCCTTAGGAACTTAA
377









5. Treatment of Diseases and Disorders

The present invention also provides methods of treating or preventing a disease or disorder in a mammal. The methods include regulating the expression of at least one gene in a cell known to cause a disease or disorder by introducing to the cells of the mammal at least one invasive bacterium, or at least one bacterial therapeutic particle (BTP), containing one or more siRNAs or one or more DNA molecules encoding one or more siRNAs, where the expressed siRNAs interfere with the mRNA of the gene known to cause the disease or disorder of interest.
The RNAi methods of the invention, including BMGS and tkRNAi are used to treat any disease or disorder for which gene expression regulation would be beneficial. This method is effected by silencing or knocking down (decreasing) genes involved with one or more diseases and disorders.
The gene to be regulated to treat or prevent a disease or disorder of interest, can be, but is not limited to, ras, β-catenin, one or more HPV oncogenes, APC, HER-2, MDR-1, MRP-2, FATP4, SGLUT-1, GLUT-2, GLUT-5, apobec-1, MTP, IL-6, IL-6R, IL-7, IL-12, IL-13, IL-13 Ra-1, IL-18, p38/JNK MAP kinase, p65/NF-κB, CCL20 (MIP-3α), Claudin-2, Chitinase 3-like 1, apoA-IV, MHC class I and MHC class II. In one aspect of this embodiment, the ras is k-Ras. In another aspect of this embodiment, the HPV oncogene is E6 or E7.
Preferably, the present invention provides methods of treating or preventing cancer or a cell proliferation disorder, viral disease, an inflammatory disease or disorder, a metabolic disease or disorder, an autoimmune disease or disorder, or a disease, disorder or cosmetic concern in the skin or hair in a mammal by regulating the expression of a gene or several genes known to be associated with the onset, propagation or prolongation of the disease or disorder by introducing a bacterium or BTP to the cell. The bacterium or BTP contain one or more siRNAs or one or more DNA molecules encoding one or more siRNAs, where the expressed siRNAs interfere with the mRNA of the gene known to cause, propagate or prolong the disease or disorder of interest.
In some preferred embodiments, the viral disease can be, but is not limited to, hepatitis B, hepatitis C, Human Papilloma Virus (HPV) infection or epithelial dysplasia or cancer caused by HPV infection or HPV induced transformation, including cervical cancer, rectal cancer and pharyngeal cancer.
In some preferred embodiments, the inflammatory disease or disorder can be, but is not limited to, inflammatory bowel disease, Crohn's disease, ulcerative colitis, an allergy, rheumatoid arthritis.
In some preferred embodiments, the automimmune disease or disorder can be, but is not limited to, celiac disease.
In some preferred embodiments, the disease, disorder or cosmetic concern can be, but is not limited to, psoriasis, eczema, albinism, balding or gray hair.
The mammal can be any mammal including, but not limited to, human, bovine, ovine, porcine, feline, canine, goat, equine, or primate. Preferably, the mammal is a human.
The terms “treating” and “treatment” as used herein refer to the administration of an agent or formulation (e.g., bacterium and/or BTP containing an siRNA or a DNA encoding for an siRNA) to a clinically symptomatic individual afflicted with an adverse condition, disorder, or disease, so as to effect a reduction in severity and/or frequency of symptoms, eliminate the symptoms and/or their underlying cause, and/or facilitate improvement or remediation of damage.
The terms “preventing” and “prevention” refer to the administration of an agent or composition to a clinically asymptomatic individual who is susceptible to a particular adverse condition, disorder, or disease, and thus relates to the prevention of the occurrence of symptoms and/or their underlying cause.
6. Pharmaceutical Compositions and Modes of Administration
In a preferred embodiment of the invention, the invasive bacteria or BTPs containing the RNA molecules, and/or DNA encoding such, are introduced into an animal by intravenous, intramuscular, intradermal, intraperitoneally, peroral, intranasal, intraocular, intrarectal, intravaginal, intraosseous, oral, immersion and intraurethral inoculation routes.
The amount of the invasive bacteria or BTPs of the present invention to be administered to a subject will vary depending on the species of the subject, as well as the disease or condition that is being treated. Generally, the dosage employed will be about 103 to 1011 viable organisms, preferably about 105 to 109 viable organisms per subject.
The invasive bacteria or BTPs of the present invention are generally administered along with a pharmaceutically acceptable carrier and/or diluent. The particular pharmaceutically acceptable carrier an/or diluent employed is not critical to the present invention. Examples of diluents include a phosphate buffered saline, buffer for buffering against gastric acid in the stomach, such as citrate buffer (pH 7.0) containing sucrose, bicarbonate buffer (pH 7.0) alone (Levine et al. J. Clin. Invest., 79:888-902 (1987); and Black et al J. Infect. Dis., 155:1260-1265 (1987)), or bicarbonate buffer (pH 7.0) containing ascorbic acid, lactose, and optionally aspartame (Levine et al. Lancet, II:467-470 (1988)). Examples of carriers include proteins, e.g., as found in skim milk, sugars, e.g., sucrose, or polyvinylpyrrolidone. Typically these carriers would be used at a concentration of about 0.1-30% (w/v) but preferably at a range of 1-10% (w/v).
Set forth below are other pharmaceutically acceptable carriers or diluents which may be used for delivery specific routes. Any such carrier or diluent can be used for administration of the bacteria of the invention, so long as the bacteria or BTPs are still capable of invading a target cell. In vitro or in vivo tests for invasiveness can be performed to determine appropriate diluents and carriers. The compositions of the invention can be formulated for a variety of types of administration, including systemic and topical or localized administration. Lyophilized forms are also included, so long as the bacteria are invasive upon contact with a target cell or upon administration to the subject. Techniques and formulations generally may be found in Remmington's Pharmaceutical Sciences, Meade Publishing Co., Easton, Pa. For systemic administration, injection is preferred, including intramuscular, intravenous, intraperitoneal, and subcutaneous. For injection, the composition, e.g., bacteria or BTPs, of the invention can be formulated in liquid solutions, preferably in physiologically compatible buffers such as Hank's solution or Ringer's solution.
For oral administration, the pharmaceutical compositions may take the form of, for example, tablets or capsules prepared by conventional means with pharmaceutically acceptable excipients such as binding agents (e.g., pregelatinised maize starch, polyvinylpyrrolidone or hydroxypropyl methylcellulose); fillers (e.g., lactose, microcrystalline cellulose or calcium hydrogen phosphate); lubricants (e.g., magnesium stearate, talc or silica); disintegrants (e.g., potato starch or sodium starch glycolate); or wetting agents (e.g., sodium lauryl sulphate). The tablets may be coated by methods well known in the art. Liquid preparations for oral administration may take the form of, for example, solutions, syrups or suspensions, or they may be presented as a dry product for constitution with water or other suitable vehicle before use. Such liquid preparations may be prepared by conventional means with pharmaceutically acceptable additives such as suspending agents (e.g., sorbitol syrup, cellulose derivatives or hydrogenated edible fats); emulsifying agents (e.g., lecithin or acacia); non-aqueous vehicles (e.g., almond oil, oily esters, ethyl alcohol or fractionated vegetable oils); and preservatives (e.g., methyl or propyl-p-hydroxybenzoates or sorbic acid). The preparations may also contain buffer salts, flavoring, coloring and sweetening agents as appropriate.
Preparations for oral administration may be suitably formulated to give controlled release of the active compound. For buccal administration the compositions may take the form of tablets or lozenges formulated in conventional manner.
For administration by inhalation, the pharmaceutical compositions for use according to the present invention are conveniently delivered in the form of an aerosol spray presentation from pressurized packs or a nebuliser, with the use of a suitable propellant, e.g., dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gas. In the case of a pressurized aerosol the dosage unit may be determined by providing a valve to deliver a metered amount. Capsules and cartridges of e.g. gelatin for use in an inhaler or insufflator may be formulated containing a powder mix of the composition, e.g., bacteria, and a suitable powder base such as lactose or starch.
The pharmaceutical compositions may be formulated for parenteral administration by injection, e.g., by bolus injection or continuous infusion. Formulations for injection may be presented in unit dosage form, e.g., in ampoules or in multi-dose containers, with an added preservative. The compositions may take such forms as suspensions, solutions or emulsions in oily or aqueous vehicles, and may contain formulatory agents such as suspending, stabilizing and/or dispersing agents. Alternatively, the active ingredient may be in powder form for constitution with a suitable vehicle, e.g., sterile pyrogen-free water, before use.
The pharmaceutical compositions may also be formulated in rectal, intravaginal or intraurethral compositions such as suppositories or retention enemas, e.g., containing conventional suppository bases such as cocoa butter or other glycerides.
Systemic administration can also be by transmucosal or transdermal means. For transmucosal or transdermal administration, penetrants appropriate to the barrier to be permeated are used in the formulation. Such penetrants are generally known in the art, and include, for example, for transmucosal administration bile salts and fusidic acid derivatives. In addition, detergents may be used to facilitate permeation. Transmucosal administration may be through nasal sprays or using suppositories. For topical administration, the bacteria of the invention are formulated into ointments, salves, gels, or creams as generally known in the art, so long as the bacteria are still invasive upon contact with a target cell.
The compositions may, if desired, be presented in a pack or dispenser device and/or a kit that may contain one or more unit dosage forms containing the active ingredient. The pack may for example comprise metal or plastic foil, such as a blister pack. The pack or dispenser device may be accompanied by instructions for administration.
The invasive bacteria or BTPs containing the RNA or RNA-encoding DNA to be introduced can be used to infect animal cells that are cultured in vitro, such as cells obtained from a subject. These in vitro-infected cells can then be introduced into animals, e.g., the subject from which the cells were obtained initially, intravenously, intramuscularly, intradermally, or intraperitoneally, or by any inoculation route that allows the cells to enter the host tissue. When delivering RNA to individual cells, the dosage of viable organisms administered will be at a multiplicity of infection ranging from about 0.1 to 106, preferably about 102 to 104 bacteria per cell.
In yet another embodiment of the present invention, bacteria can also deliver RNA molecules encoding proteins to cells, e.g., animal cells, from which the proteins can later be harvested or purified. For example, a protein can be produced in a tissue culture cell.
While the invention has been described in conjunction with the detailed description thereof, the foregoing description is intended to illustrate and not limit the scope of the invention, which is defined by the scope of the appended claims. Other aspects, advantages, and modifications are within the scope of the following claims.
The present invention is further illustrated by the following examples that should not be construed as limiting in any way. The contents of all cited references including literature references, issued patents, published patent applications as cited throughout this application are hereby expressly incorporated by reference. The practice of the present invention will employ, unless otherwise indicated, conventional techniques of cell biology, cell culture, molecular biology, transgenic biology, microbiology, recombinant DNA, and immunology, which are within the skill of the art. Such techniques are explained fully in the literature. See, for example, Molecular Cloning A Laboratory Manual, 2nd Ed., ed. by Sambrook, Fritsch and Maniatis (Cold Spring Harbor Laboratory Press: 1989); DNA Cloning, Volumes I and II (D. N. Glover ed., 1985); Oligonucleotide Synthesis (M. J. Gait ed., 1984); Mullis et al. U.S. Pat. No. 4,683,195; Nucleic Acid Hybridization (B. D. Hames & S. J. Higgins eds. 1984); Transcription And Translation (B. D. Hames & S. J. Higgins eds. 1984); Culture Of Animal Cells (R. I. Freshney, Alan R. Liss, Inc., 1987); Immobilized Cells And Enzymes (IRL Press, 1986); B. Perbal, A Practical Guide To Molecular Cloning (1984); the treatise, Methods In Enzymology (Academic Press, Inc., N.Y.); Gene Transfer Vectors For Mammalian Cells (J. H. Miller and M. P. Calos eds., 1987, Cold Spring Harbor Laboratory); Methods In Enzymology, Vols. 154 and 155 (Wu et al. eds), Immunochemical Methods In Cell And Molecular Biology (Mayer and Walker, eds., Academic Press, London, 1987); Handbook Of Experimental Immunology, Volumes I-IV (D. M. Weir and C. C. Blackwell, eds., 1986); Manipulating the Mouse Embryo, (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1986).
The following non-limiting examples are merely illustrative of the preferred embodiments of the present invention, and are not to be construed as limiting the invention.
EXAMPLES
Example 1
Knockdown of β-Catenin and k-Ras
Previous studies have demonstrated the powerful nature of the siRNA knockdown technology disclosed herein. For example, in vitro and in vivo knockdown of beta catenin and k-ras utilizing bacterial delivery is described in PCT Publication No. WO 06/066048, which is incorporated herein by reference in its entirety.
Example 2
TRIP with Multiple shRNA Expression Cassettes
The TRIP described herein, and described in further detail in PCT Publication No. WO 06/066048, can be modified to produce a plasmid which allows targeting of multiple genes simultaneously or multiple sequences within one gene simultaneously. For example, TRIP with multiple hairpin expression cassettes to produce shRNA can target different sequences in a given gene, or target multiple genes through a simultaneous bacterial treatment.
The TRIP plasmid can incorporate multiple (up to ten) cloning sites to express different shRNA constructs (FIG. 1). The purpose of such a plasmid will be to allow silencing of various genes through a single therapeutic bacterium which will be empowered by the Multiple-expression cassette-TRIP (mec-TRIP) to synthesize short hairpin RNA against a variety of targets simultaneously.
These different hairpins can either be expressed competitively at high levels through the use of an identical high level promoter (such as T7 promoter or a different high level bacterial promoter), or they can be expressed at different levels through the use of promoters with different levels of activity, this will depend on the intended use of the plasmid and the desired relative silencing levels of the target gene.
This mec-TRIP could be useful to treat complex diseases as described herein (e.g. inflammatory diseases, or cancer), through the simultaneous silencing(targeting) of multiple targets as described herein (e.g. multiple oncogenes, such as k-ras and beta-catenin in the case of colon cancer, or HER-2 and MDR-1 in breast cancer, or other combinations).
Example 3
Operator Repressor Titration System
The TRIP system (bacteria and plasmid) have been modified to include the ORT (Operator Repressor Titration) system from Cobra Biomanufacturing (Keele, UK). This adaptation helps to maintain the plasmid in suitable strains in the absence of selective antibiotics. The bacterial carrier strain has been modified accordingly to allow for the ORT system to function (deletion of the DAP gene and replacement with an ORT-controlled DAP gene expression system). The plasmid has been modified to remove the antibiotic selection sequences to support the ORT system. Further changes have been introduced to the bacterial genome, including for example, (a) deletion of the aroA gene (in some CEQ strains) to make the bacteria more susceptible to nutrient shortage, particularly in the intracellular compartment where they will die due to lack of nutrients; (b) insertion of T7RNApolymerase gene into the chromosome and or (c) integration of a shRNA expression cassette under T7 promoter into the chromosome.
FIG. 2 shows development examples of bacterial strains. Further strains developed include, but are not limited to, CEQ922 (CEQ919 without aroA deletion), CEQ923 (CEQ920 without aroA deletion), CEQ924 (CEQ921 without aroA deletion).
Example 4
Intestinal Tract Gene Delivery
S. typhimurium was investigated to determine if it could be used as a vector for RNAi delivery into the epithelial cells lining the intestinal tract. Mice were treated orally with a single dose of 108 SL 7207 and sacrificed at various time points after administration. SL7207 were then stained using the Salmonella specific antibody. 2 h after treatment, numerous SL7207 could be seen invading the intestinal epithelial layer (Salmonella stained red), suggesting that oral administration of SL7207 may be a useful tool to deliver payloads to the intestinal and colonic mucosa. In a follow up experiment, mice were treated with SL7207 harboring a GFP expression plasmid (pEGFPC1, Invitrogen). At 24 h after a single treatment, a small percentage (approximately 1%) of cells was clearly found to express GFP.
FIG. 3 shows the efficient invasion and plasmid delivery into the intestinal mucosa by S. typhimurium. SL7207 were stained using red fluorescent antibody 6 h after oral administration. Intact SL7207 and fragments of SL7207 were seen in epithelial cells as well as underlying cells of the lamina propria (top left/right). SL7207 successfully deliver expressed DNA into the intestinal mucosa: intestinal mucosal cells expressing GFP after treatment with SL7207 carrying a eukaryotic expression plasmid for GFP (pEGFP-C1) (lower left). For fluorescence microscopy, SL7207 were stained with red fluorescent antibody and nuclei were counterstained with Hoechst 37111.
To test whether SL7207 could be used for the delivery of RNAi to target genes in the intestinal tract, GFP transgenic mice (4 per group) were treated with S. typhimurium harboring a shRNA expression plasmid directed against GFP (SL-siGFP) or a shRNA expression plasmid directed against k-RAS (SL-siRAS). 108 c.f.u. was given three times weekly for two weeks by oral gavage. Colonic tissues were subsequently reviewed with fluorescent microscopy (data not shown) and stained analyzed after immunhistochemistry staining for GFP expression using a specific antibody (Living Colors®, Invitrogen). There was a significant reduction in the overall GFP expression level and significant reduction in the number of GFP expressing crypts in the SL-siGFP treated animals compared with the SL-siRAS treated animals (33.9% vs 50%, p<0.05), suggesting that this method could be useful to deliver therapeutic RNAi into the colonic epithelium.
FIG. 4 shows that bacteria-mediated RNA interference reduces target gene expression in the gastrointestinal epithelium. After treatment with SL7207 carrying expression plasmids targeting GFP (SL-siGFP, right bottom panel), colon tissues showed lower levels of GFP expression, and fewer colonic crypts were stained positive for GFP compared with animals treated with SL-siRAS (left bottom panel). Slides were stained with GFP-specific antibody.
Example 5
Construction of CEQ503 Bacterial Strain
Derivation and Description of CEQ 503 (Strain CEQ201 (pNJSZ))
CEQ503 consists of a combination of an attenuated E. coli strain (CEQ201) with a specially engineered TRIP plasmid (pNJSZ). The plasmid confers the abilities required to induce tkRNAi (in this case: invasiveness, escape from the entry vesicle, expression of short hairpin RNA). Strain Description of CEQ503 (pNJSZ):

    1. Genotype: Escherichia coli CEQ201 [glnV44(AS), LAM−, rfbC1, endA1, spoT1, thi-1, hsdR17, (rk−mk+),creC510 ΔdapA, ΔrecA].
    2. Derivation of CEQ201






    3. Plasmid: pNJSZ, shown schematically in FIG. 5, is a 10.4 kb plasmid that confers kanamycin resistance to our bacterial strain (CEQ503). This plasmid contains two genes, hly and inv, and the H3 hairpin sequence: ggatccAGGAGTAACAATACAAATGGATTCAAGAGATCCATTTGTATTGTTACTCCTTTgt cgac (SEQ ID NO:380), which includes BamHI and SaII restriction sites. To verify the presence of this plasmid, PCRs are performed to verify chromosomal deletion of dapA, and minipreps and/or PCR are performed to confirm inv, hly and 341-H3 on the plasmid.
    4. Nutritional Requirements: Althea Media Broth or LB, Miller (Luria-Bertani) broth (Amresco; cat. no.: J106-2KG) and 50 μg/ml of DL-Δ;ε-Diaminopemilic acid (DAP) (SIGMA; cat. no.: D1377-10G).
    5. Growth Conditions: 37° C.


Example 6
BTP Production
BTPs or minicells containing a suitable plasmid such as TRIP have been engineered for delivery of tkRNAi. These cells will express invasin or Opa to enable entry into mammalian cells and listeriolysin will allow lysis of phagosome following minicell degradation/lysis. Additionally, a method for manufacturing minicells has been developed that utilizes a suicide construct for killing intact cells to aid in the purification of minicells. Such suicide plasmids have been described in the literature (Kloos et al., (1994) J. Bacteriol. 176, 7352-61; Jain and Mekalanos, (2000) Infect. Immun 68, 986-989). Summarily, the lambda S and R genes that code for holing and lysozyme are placed under regulation of an inducible promoter on the bacterial chromosome. When induced, they will lyse intact cells but not minicells since minicells lack chromosomes. A number of different types of regulators such as lad, araC, lambda cI857 and rhaS-rhaR can be used for development of an inducible suicide gene construct. Similarly, a number of different types of suicide genes, including E. coli autolysis genes and antimicrobial small peptides, can be used in a similar scheme. Purification is enhanced by treatments or mutations that induce filamentation (see, for example, Ward and Lutkenhaus, (1985) Cell 42, 941-949; Bi and Lutkenhaus, 1992). Initial purification involves low speed centrifugation to separate intact cells and retain minicells in the supernatant. This can be followed by density gradient purification or filtration (for example, Shull et al., (1971) J. Bacteriol. 106, 626-633).
Any cell death-triggering gene, also known as a suicide gene, including but not limited to genes encoding antimicrobial proteins, bacteriophage lysins or autolysins can be used in this method for obtaining BTPs from a mixture containing BTPs and bacteria. Suicide genes can kill live bacteria by mechanisms that include but are not limited to cell lysis, or by the destruction, degradation or poisoning of cellular components such as chromosomal DNA or filament components. Any inducible promoter may be used in conjunction with this system. In one embodiment of this invention, the suicide genes are integrated within the chromosome, thereby limiting their presence only in intact bacterial cells as BTPs or minicells will not incorporate these genes because they do not harbor chromosomal DNA.
As shown in FIG. 6, induction of suicide genes will lyse intact bacterial cells. The lambda S and R genes (suicide genes) are put under the control of PlacUV5 (inducible promoter). The leaky basal activity is repressed by a “super-repressor” coded by lacIq gene on a PgapA (strong promoter). This cassette is put at the minCD locus.
Example 7
siRNA Inhibition of Human Papillomavirus (HPV) Oncogenes
Experimental Procedures
Cell Culture: Hela cells were cultured in Minimum Essential Medium (MEM, ATCC No. 30-2003) with 10% FBS supplemented with antibiotics: 100 U/ml penicillin G, 10 μg/ml streptomycin (Sigma).
Bacterial Culture: Plasmids were transformed into BL21(DE3) strain (Invitrogen). Bacteria were grown at 37° C. in LB Broth containing 100 μg/ml ampicillin. Bacterial cell density (in CFU/ml) was calculated using OD600 measurement. For cell infection, overnight cultures were inoculated into fresh medium for another 2-3 h growth until the optical density at 600 nm [OD600] reached 0.6. Invasion Assay: For bacterial invasion, Hela cells were plated in 6-well dishes at 200,000 cells/well and allowed to incubate overnight in 2 ml complete growth medium. The bacterial cells were grown to mid-exponential phase with optical density at 600 nm [OD600] 0.6 in LB Broth with Ampicillin, and then centrifuged at 3,400 rpm for 10 minutes at 4° C. Bacterial pellets were resuspended in MEM without serum or the antibiotics and the bacteria were added to the cells at an MOI of 1:1000, 1:500, 1:250, 1:125, or 1:62.5 and allowed to invade the Hela cells for 2 hours at 37° C. in 5% CO2. The cells were washed 4 times with MEM containing 10% FBS and penicillin-streptomycin (100 IU of penicillin and 100 μg of streptomycin per ml). Cells were incubated in fresh complete medium for further 48 hours at 37° C. in 5% CO2 and total RNA was then isolated by the Qiagen RNeasy system with on-column DNAse digestion or by TRIZOL extraction method.
siRNA Transfection: One day before the transfection, cells were plated in complete growth medium without antibiotics so that the cells will be 30-50% confluent at the time of transfection. Diluted various concentrations of siRNA from a stock of 20 μM in 175 μl of Opti-MEM. Mixed 4 μl of Oligofectamine separately in 15 μl of Opti-MEM. Mixed gently and incubated for 5-10 min at room temperature. Combined the diluted siRNA with diluted oligofectamine and incubated for 15-20 min at room temperature. While the complexes were being formed, removed the growth medium from the cells and added 800 μl of medium without serum to each well containing cells. Added the 200 μl of siRNA/oligofectamine complexes to the cells and incubated at 37° C. for 4 h. Added 1 ml of growth medium containing 3× the normal concentration of serum without removing the transfection mixture. Gene silencing was assayed at 48 h.
RT-PCR: Quantitative real-time reverse transcription PCR (RT-PCR) was performed with the TaqMan RT-PCR master Mix Reagents Kit (Applied Biosystems) using the following primers and a probe set for detection of HPV18E6E7 transcripts:









Forward Primer:



(SEQ ID NO: 381)



5′-CTGATCTGTGCACGGAACTGA-3′ (148-168)



 



Reverse Primer:



(SEQ ID NO: 382)



5′-TGTCTAAGTTTTTCTGCTGGATTCA-3′(439-463)



 



Probe:



(SEQ ID NO: 383)



5′-TTGGAACTTACAGAGGTGCCTGCGC-3′ (219-233 and



 



416-425)






The probe was labeled at the 5′ end with a reporter fluorescent dye, FAM and at the 3′ end with fluorescent dye quencher TAMRA. GAPDH was used to detect human GAPDH transcripts for the normalization.








HPVsHRNA sequences:


H1 (working sequence)


(SEQ ID NO: 384)


5′- ggATCCTAGGTATTTGAATTTGCATTTCAAGAGAATGCAAATTCAA


 


ATACCTTTTgTCgAC


 


(SEQ ID NO: 385)


5′- GTCGACAAAAGGTATTTGAATTTGCATTCTCTTGAAATGCAAATTC


 


AAATACCTAGGATCC


 


H2 (ineffective sequence)


(SEQ ID NO: 386)


5′-ggATCCTCAGAAAAACTTAGACACCTTCAAGAGAGGTGTCTAAGTTT


 


TTCTGTTTgTCgAC


 


(SEQ ID NO: 387)


5′- GTCGACAAACAGAAAAACTTAGACACCTCTCTTGAAGGTGTCTAAG


 


TTTTTCTGAGGATCC






Western Blot: Hela cells were lysed using 1× Cell lysis Buffer (Cell Signaling Technology, Cat No. 9803). For electrophoresis, 50 μg of total protein in 2× loading buffer was loaded to each well of a 12% SDS-PAGE gel. After transferring the blot was blocked and probed with primary antibody at 2 h followed by incubation with HRP-conjugated secondary antibody before detection by ECL. All primary antibodies were used at 1/1000 dilution except HPV18E7 antibody at 1/250. Anti-Human pRb antibody: BD Pharmingen (Cat No. 554136), Sec Ab: HRP-anti Mouse HPV18E7: Santa Cruz (Cat No. sc-1590), Sec Ab: donkey anti-goat IgG-HRP Cat no. sc 2020

    p53: Santa Cruz (Cat No. sc-126), Sec Ab: HRP-anti Mouse
    p21: Santa Cruz (Cat No. sc-397), Sec Ab: HRP-anti Rabbit
    c-Myc: Cell Signaling Technology (Cat No. 9402), Sec Ab: HRP-anti Rabbit


Colony Formation Assay: Hela cells were harvested after bacterial invasion for 2 h. The cells in either control treated or HPV shRNA treated cells were washed 3× times with complete MEM and one time with PBS. The cells were then trypsinized and counted. 500 cells from each treatment were added to a single well of a six well plate containing 2 ml of complete growth medium. The cells were allowed to grow for 10 days following which the colonies were fixed with GEIMSA stain.
MTT Assay: Hela cells were harvested after bacterial invasion for 2 h. The cells in either control treated or HPV shRNA treated cells were washed 3× times with complete MEM and one time with PBS. The cells were then trypsinized and counted. 5000 cells from each treatment were added to a single well of a 96 well plate in 100 μl of complete growth medium in triplicates. The cells were incubated at 37° C. for 48-72 h following which 10 μl of 0.5 mg/ml MTT was added to each well. The plate was further incubated at 37° C. for 3 h, the medium was aspirated off from the wells and after incubation, 100 μl of MTT solubilization solution [10% Triton X-100 in acidic isoproponal (0.1 N HCl)] was added to each well to stop the reaction. The absorbance was read at 570 nm on the plate reader.
Results
In this example, the suppressive effect of a short hairpin RNA directed towards HPV 18 E6 and E7 oncogenes was investigated. The short hairpin RNA was delivered by infecting human cervical cancer cells (Hela) with bacterial strains that produce the short hairpin RNA. The shRNA expression cassette contained 19 nucleotide (nt) of the target sequence followed by the loop sequence (TTCAAGAGA) (SEQ ID NO:388) and the reverse complement to the 19 nt. For the 19 nt, two shRNA sequences published in Cancer Gene Therapy (2006) 13, 1023-1032, were used to measure siRNA delivery and gene silencing efficiency, oligofectamine reagent in a 6 well format was used. Briefly, Hela cells were plated at a cell density of about 40% confluence in antibiotic free medium. On the next day, siRNA was added to 6 well plates at varying concentrations of 50, 100, 200 nM. The control siRNA was added at a single concentration of 100 nM.
As shown in FIG. 7, the oligofectamine transfection method resulted in a decrease in E6 mRNA in Hela cells with respect to the control siRNA. The siRNA (H1) showed up to about 40% of reduction in E6 mRNA. The knockdown response was not dose dependent.
Next, the hairpin of the siRNA (H1) was cloned into the TRIP vector. In order to determine if gene silencing could be achieved through the transkingdom system, the shRNA in human cervical cancer cells (Hela) was tested in an invasion assay. Briefly, Hela cells were plated in a six-well plate at 2×105 cells/well, allowed to grow overnight and incubated the next day for 2 h at different MOIs with bacteria (E. coli) engineered to produce the hairpin RNA. The bacteria were washed off with medium containing 10% FBS and Pen Strep four times and the mammalian cells were further incubated for an additional 48 h in the complete medium. RNA or protein was isolated from the bacteria.
FIG. 8 and FIG. 9 demonstrate that siRNA downregulates HPV E6 expression in Hela cells. Cells were plated in six well plates and allowed to grow to a confluence of 40% (about 40,000 cells). Oligofectamine/siRNA transfection complexes were prepared in Opti-MEM serum-free medium by mixing 4 μl of oligofectamine with siRNAs (final concentration in 185 μl of medium is 50, 100, 200 nM). 48 hours post-transfection cells were harvested and analyzed by real-time RT-PCR for both target and GAPDH mRNA levels. Data were normalized against the GAPDH signal. Two different negative control siRNAs were used at a single concentration of 200 nM.
FIG. 10, Panels A-C show real time PCR results following invasion assay of Hela cells. Hela cells were incubated for 2 h with shRNA-expressing BL21(DE3) at different multiplicities of infection (MOI). Forty-eight hours post-infection the cells were harvested and analyzed by real-time RT-PCR for both target and GAPDH mRNA levels. Data were normalized against the GAPDH signal. These data were then further normalized to untreated control cells.
FIG. 11 shows the effects of downregulation of HPV E6 and E7 genes on tumor suppressor pathways and other downstream targets. Hela cells were incubated for 2 h with shRNA-expressing BL21(DE3) at different multiplicities of infection (MOI). Forty-eight hours post-infection cells were harvested and analyzed by western blotting. 50 μg of protein was loaded in each lane and resolved by gel electrophoresis, transferred to a membrane and probed with antibodies specific for HPV 18 E7, p53, actin, p110Rb, p21 and c-myc as indicated.
FIGS. 12 and 13 show a colony formation and MTT assay, respectively. Hela cells were incubated for 2 h with shRNA-expressing BL21(DE3) at different multiplicities of infection (MOI). 2 h post-infection cells were washed trypsinized and counted and an equal number of cells for each MOI was added to a well of a six well plate (For CFA: added 500 cells to each well of a 6 well plate, for MTT added 5000 cells in each well of a 96 well plate). For colony formation, the cells were allowed to grow for 10 days and stained with Geimsa, MTT assay was analyzed at 72 h post plating.
FIGS. 14 and 15 show real time PCR results following invasion assay of Hela cells. Hela cells were incubated for 2 h with shRNA-expressing BL21(DE3) at different multiplicities of infection (MOI). Forty-eight hours post-infection cells were harvested and analyzed by real-time RT-PCR for both target and GAPDH mRNA levels. Data were normalized against the GAPDH signal. These data were then further normalized to untreated control cells.
FIG. 16 shows the effects of downregulation of HPV E6 and E7 genes on tumor suppressor pathways and other downstream targets. Hela cells were incubated for 2 h with shRNA-expressing BL21(DE3) at different multiplicities of infection (MOI). Forty-eight hours post-infection cells were harvested and analyzed by western blotting. 50 μg of protein was loaded in each lane and resolved by gel electrophoresis, transferred to a membrane and probed with antibodies specific for HPV 18 E7, p53, actin, p110Rb as indicated.
FIG. 17 shows real time PCR results following invasion assay of Hela cells with a frozen aliquot of negative sHRNA control and HPV sHRNA in BL21 (DE3). Hela cells were incubated for 2 h with shRNA-expressing BL21(DE3) at different multiplicities of infection (MOI). Forty-eight hours post-infection cells were harvested and analyzed by real-time RT-PCR for both target and GAPDH mRNA levels. Data were normalized against the GAPDH signal. These data were then further normalized to untreated control cells.
FIG. 18 shows the plating efficiency of frozen aliquots of negative sHRNA control and HPV sHRNA in BL21 (DE3). The frozen bacteria were thawed and resuspended to a final concentration of 3.38×108 cells/ml. Invasion assay was performed with this concentration taking 2 mls of 3.38×108 cells/ml as an MOI of 1000. Some stock control bacteria or HPV bacteria were serially diluted (1:100) and plated on LB plates to assess for the number and viability of bacteria treated cells at 48 h. Gene silencing was analyzed either by quantitative real-time PCR using the ΔΔCt relative quantitation method or by western blot analysis. HPVE6 mRNA levels were normalized to an endogenous control, GAPDH. The final data were further normalized to the RNA from the untreated cells. For Protein analysis, cell lysates were prepared in Cell Lysis Buffer (Cell Signaling Technology) and the protein concentration was determined using a BCA kit from BioRad. For electrophoresis, the protein expression was normalized to Actin loading control.
Example 8
Knockdown of HPV E6 Gene Assessed by Western Blotting with HPV 18 E7 Antibody
Hela cells were incubated for 2 h with shRNA-expressing BL21(DE3) (HPVH1 construct below) at different multiplicities of infection (MOI). Forty-eight hours post-infection cells were harvested and analyzed by western blotting. The HPV E6 specific knockdown was compared with a negative shRNA control. Briefly, 50 μg of protein was loaded in each lane and resolved by gel electrophoresis, transferred to a membrane and probed with antibodies specific for HPV 18 E7, and actin as indicated.









HPVH 1



(SEQ ID NO: 389)



5′-GATCC TAGGTATTTGAATTTGCAT TTCAAGAGA



 



ATGCAAATTCAAATACCTTTT G-3′



 



(SEQ ID NO: 390)



3′-G ATCCATAAACTTAAACGTA AAGTTCTCT



 



TACGTTTAAGTTTATGGAAAA CAGCT-5′






FIG. 19 shows the knockdown of HPV E6 gene assessed by western blotting with HPV 18 E7 antibody. Hela cells were incubated for 2 h with shRNA-expressing BL21 (DE3) at different multiplicities of infection (MOI). Forty-eight hours post-infection cells were harvested and analyzed by western blotting. The HPV E6 specific knockdown was compared with a negative sHRNA control. Briefly, 50 μg of protein was loaded in each lane and resolved by gel electrophoresis, transferred to a membrane and probed with antibodies specific for HPV 18 E7 and actin as indicated.
Example 9
Inhibition of CCL20 Expression in CMT93 Cells
One confluent T-175 flask of CMT93 cells was trypsinized in 10 mls until the cells detached. Trypsin was inactivated by addition of 30 mls of DMEM (10% FCS, pen/strep) and the cells thoroughly mixed by pipetting. From this solution, 8 mls was transferred into a sterile 50 ml tube and 32 mLs of DMEM 10% added. Cells were well mixed and 250 uLs added to each well of a 48 well plate and incubated overnight at 37 C resulting in adherent cells that were approximately 70% confluent the following morning. The next day, siRNA transfection complexes were created by the following method.
Sequences were ordered from Qiagen as pre-annealed siRNA duplexes. Each well was resuspended in 250 ul of siRNA buffer (from Qiagen) to give a stock concentration of 20 uM. The plate was then placed in a water bath at 95 C for 5 minutes and then allowed to slowly cool to resuspend the duplexes and break apart aggregates. The suspended duplexes were then used in transfection experiments described in standard protocols. The formulation is per well of a 48 well plate containing 250 uL of media; each screen was performed in biological triplicate so the solution was made for 4 wells; 3 for transfection and 1 extra.
0.3 uL of the appropriate siRNA (from a 20 uM stock solution) were diluted to 47 uL with serum/antibiotic free media and mixed. To this solution was added 3 uL of HiPerfect transfection reagent (Qiagen) followed by brief vortexing and incubation at room temperature for 20 minutes. 50 uLs of the complex containing mixture was added to each of 3 wells in a 48 well plate containing CMT93 cells. Transfection was for 24 hours at 37 C at which time the media was removed and replaced with 400 uLs of DMEM/10% FCS containing 10Ong/mL of LPS for 2 hours. Following stimulation, the cells were washed and RNA isolated for qRT-PCR according the Qiagen Quantitech method (see manufacturer's protocol) for 50 cycles.
FIG. 20 shows the knockdown of CCL20 expression with the various siRNA sequences in CMT93 cells. The siRNA sequences tested are listed below:
















SEQ ID

SEQ ID


Well
siRNA sense 5′→3′
NO:
siRNA antisense 5′→3′
NO:







G1
GCUUGUGACAUUAAUGCUAtt
391
UAGCAUUAAUGUCACAAGCtt
392


 


H1
AUAAGCUAUUGUAAAGAUAtt
393
UAUCUUUACAAUAGCUUAUtg
394


 


G2
CAUCUUUCACACGAAGAAAtt
395
UUUCUUCGUGUGAAAGAUGat
396


 


H2
CUAUUGUAAAGAUAUUUAAtt
397
UUAAAUAUCUUUACAAUAGct
398


 


G3
GCCUAAGAGUCAAGAAGAUtt
399
AUCUUCUUGACUCUUAGGCtg
400


 


G4
CAGUGGACUUGUCAAUGGAtt
401
UCCAUUGACAAGUCCACUGgg
402


 


G5
GAAGUUGAUUCAUAUUGCAtt
403
UGCAAUAUGAAUCAACUUCat
404


 


G6
GUUGAUUCAUAUUGCAUCAtt
405
UGAUGCAAUAUGAAUCAACtt
406


 


G7
ACAUUAGAGUUAAGUUGUAtt
407
UACAACUUAACUCUAAUGUga
408


 


G8
CAUUAGAGUUAAGUUGUAUtt
409
AUACAACUUAACUCUAAUGtg
410


 


G9
UGUUAUUUAUAGAUCUGAAtt
411
UUCAGAUCUAUAAAUAACAta
412


 


G10
GUUUAGCUAUUUAAUGUUAtt
413
UAACAUUAAAUAGCUAAACat
414


 


G11
AGUGGAAGGAUUAAUAUUAtt
415
UAAUAUUAAUCCUUCCACUaa
416


 


F12
CCAGCACUGAGUACAUCAAtt
417
UUGAUGUACUCAGUGCUGGgt
418


 


G12
UGUUUAAGGGAAUAGUUUAtt
419
UAAACUAUUCCCUUAAACAta
420









Example 10
Inhibition of Expression of Claudin-2 in CMT93 Cells
One confluent T-175 flask of CMT93 cells was trypsinized in 10 mls until the cells detached. Trypsin was inactivated by addition of 30 mls of DMEM (10% FCS, pen/strep) and the cells thoroughly mixed by pipetting. From this solution, 8 mls was transferred into a sterile 50 ml tube and 32 mLs of DMEM 10% added. Cells were well mixed and 250 uL added to each well of a 48 well plate and incubated overnight at 37 C resulting in adherent cells that were approximately 70% confluent the following morning. The next day, siRNA transfection complexes were created by the following method:
Sequences were ordered from Qiagen as pre-annealed siRNA duplexes. Each well was resuspended in 250 ul of siRNA buffer (from Qiagen) to give a stock concentration of 20 uM. The plate was then placed in a water bath at 95 C for 5 minutes and then allowed to slowly cool to resuspend the duplexes and break apart aggregates. The suspended duplexes were then used in transfection experiments described in standard protocols. The formulation is per well of a 48 well plate containing 250 uL of media; each screen was performed in biological triplicate so the solution was made for 4 wells; 3 for transfection and 1 extra.
0.3 uL of the appropriate siRNA (from a 20 uM stock solution) to 47 uL of serum/antibiotic free media and mixed. To this solution was added 3 uL of HiPerfect transfection reagent (Qiagen) followed by brief vortexing and incubation at room temperature for 20 minutes. 50 uLs of the complex containing mixture was added to each of 3 wells in a 48 well plate containing CMT93 cells. Transfection was for 24 or 48 hours at 37 C at which time the cells were washed and RNA isolated for qRT-PCR according the Qiagen Quantitech method (see manufacturer's protocol) for 50 cycles.
FIG. 21 shows the knockdown of Claudin-2 expression with the various siRNA sequences in CMT93 cells post 24 hr transfection. The siRNA sequences tested are listed below:
















SEQ ID

SEQ ID


Well
siRNA sense 5′→3′
NO:
siRNA antisense 5′→3′
NO:







D4
GCUGGGACUAUAUAUAUAAtt
421
UUAUAUAUAUAGUCCCAGCca
422


 


D5
GGGCAAUUGCUAUAUCUUAtt
423
UAAGAUAUAGCAAUUGCCCtc
424


 


D6
GCAGCCAAACGACAAGCAAtt
425
UUGCUUGUCGUUUGGCUGCtg
426


 


D7
AGGGUUUCCUUAAGGACAAtt
427
UUGUCCUUAAGGAAACCCUtg
428


 


C9
GAAAUGGAUUAGUCAGUAAtt
429
UUACUGACUAAUCCAUUUCtt
430


 


D11
GGCUCCGAAGAUACUUCUAtt
431
UAGAAGUAUCUUCGGAGCCtg
432









Example 11
Inhibition of Expression of IL6-Ra in CMT93 Cells
One confluent T-175 flask of CMT93 cells was trypsinized in 10 mls until the cells detached. Trypsin was inactivated by addition of 30 mls of DMEM (10% FCS, pen/strep) and the cells thoroughly mixed by pipetting. From this solution, 8 mls was transferred into a sterile 50 ml tube and 32 mLs of DMEM 10% added. Cells were well mixed and 250 uLs added to each well of a 48 well plate and incubated overnight at 37 C resulting in adherent cells that were approximately 70% confluent the following morning. The next day, siRNA transfection complexes were created by the following method:
Sequences were ordered from Qiagen as pre-annealed siRNA duplexes. Each well was resuspended in 250 ul of siRNA buffer (from Qiagen) to give a stock concentration of 20 uM. The plate was then placed in a water bath at 95 C for 5 minutes and then allowed to slowly cool to resuspend the duplexes and break apart aggregates. The suspended duplexes were then used in transfection experiments described in standard protocols. The formulation is per well of a 48 well plate containing 250 uL of media; each screen was performed in biological triplicate so the solution was made for 4 wells; 3 for transfection and 1 extra.
0.3 uL of the appropriate siRNA (from a 20 uM stock solution) to 47 uL of serum/antibiotic free media and mixed. To this solution was added 3 uL of HiPerfect transfection reagent (Qiagen) followed by brief vortexing and incubation at room temperature for 20 minutes. 50 uLs of the complex containing mixture was added to each of 3 wells in a 48 well plate containing CMT93 cells. Transfection was for 24, 48 or 72 hours at 37 C at which time the cells were washed and RNA isolated for qRT-PCR according the Qiagen Quantitech method (see manufacturer's protocol) for 40 cycles.
FIG. 22 shows the knockdown of IL6-RA expression with the various siRNA sequences in CMT93 cells post 24 hr transfection. The siRNA sequences tested are listed below:
















SEQ ID

SEQ ID


Well
siRNA sense 5′→3′
NO:
siRNA antisense 5′→3′
NO:







E1
CCUGGAGGGUGACAAAGUAtt
433
UACUUUGUCACCCUCCAGGat
434


 


F1
GGUCUGACAAUACCGUAAAtt
435
UUUACGGUAUUGUCAGACCca
436


 


F2
GCUGUUUCCUAUAACAGAAtt
437
UUCUGUUAUAGGAAACAGCgg
438


 


F3
GCUGUGAAAGGGAAAUUUAtt
439
UAAAUUUCCCUUUCACAGCag
440


 


E4
CCUUGUGGUAUCAGCCAUAtt
441
UAUGGCUGAUACCACAAGGtt
442


 


E5
GCUUCGAUACCGACCUGUAtt
443
UACAGGUCGGUAUCGAAGCtg
444


 


E6
CGGCAGGAAUCCUCUGGAAtt
445
UUCCAGAGGAUUCCUGCCGgg
446


 


E7
CCACGAGGAUCAGUACGAAtt
447
UUCGUACUGAUCCUCGUGGtt
448


 


E8
CACGAGGAUCAGUACGAAAtt
449
UUUCGUACUGAUCCUCGUGgt
450


 


E9
GAUCAGUACGAAAGUUCUAtt
451
UAGAACUUUCGUACUGAUCct
452


 


E10
GUACGAAAGUUCUACAGAAtt
453
UUCUGUAGAACUUUCGUACtg
454


 


E11
GAAAGUUCUACAGAAGCAAtt
455
UUGCUUCUGUAGAACUUUCgt
456


 


E12
GGGUCUGACAAUACCGUAAtt
457
UUACGGUAUUGUCAGACCCag
458









Example 12
Inhibition of Expression of IL13-Ra1 in CMT93 Cells
One confluent T-175 flask of CMT93 cells was trypsinized in 10 mls until the cells detached. Trypsin was inactivated by addition of 30 mls of DMEM (10% FCS, pen/strep) and the cells thoroughly mixed by pipetting. From this solution, 8 mls was transferred into a sterile 50 ml tube and 32 mLs of DMEM 10% added. Cells were well mixed and 250 uLs added to each well of a 48 well plate and incubated overnight at 37 C resulting in adherent cells that were approximately 70% confluent the following morning. The next day, siRNA transfection complexes were created by the following method:
Sequences were ordered from Qiagen as pre-annealed siRNA duplexes. Each well was resuspended in 250 ul of siRNA buffer (from Qiagen) to give a stock concentration of 20 uM. The plate was then placed in a water bath at 95 C for 5 minutes and then allowed to slowly cool to resuspend the duplexes and break apart aggregates. The suspended duplexes were then used in transfection experiments described in standard protocols. The formulation is per well of a 48 well plate containing 250 uL of media; each screen was performed in biological triplicate so the solution was made for 4 wells; 3 for transfection and 1 extra.
0.3 uL of the appropriate siRNA (from a 20 uM stock solution) to 47 uL of serum/antibiotic free media and mixed. To this solution was added 3 uL of HiPerfect transfection reagent (Qiagen) followed by brief vortexing and incubation at room temperature for 20 minutes. 50 uLs of the complex containing mixture was added to each of 3 wells in a 48 well plate containing CMT93 cells. Transfection was for 24 or 72 hours at 37 C at which time the cells were washed and RNA isolated for qRT-PCR according the Qiagen Quantitech method (see manufacturer's protocol) for 40 cycles.
FIG. 23 shows the knockdown of IL13-RA1 expression with the various siRNA sequences in CMT93 cells post 24 hr transfection. The siRNA sequences tested are listed below:
















SEQ ID

SEQ ID


Well
siRNA sense 5′→3′
NO:
siRNA antisense 5′→3′
NO:







F1
AGAAGACUCUAAUGAUGUAtt
459
UACAUCAUUAGAGUCUUCUtg
460


 


F2
CAGUCAGAGUAAGAGUCAAtt
461
UUGACUCUUACUCUGACUGtg
462


 


F8
CAGAACAUCUAGCAAACAAtt
463
UUGUUUGCUAGAUGUUCUGtg
464


 


F9
CUUGUAGGUUCACAUAUUAtt
465
UAAUAUGUGAACCUACAAGtt
466


 


F10
CAGUGUAGUGCCAAUGAAAtt
467
UUUCAUUGGCACUACACUGag
468


 


F11
GUAUGACAUCUAUGAGAAAtt
469
UUUCUCAUAGAUGUCAUACtt
470









Example 13
Inhibition of Expression of IL-18 in CMT93 Cells
One confluent T-175 flask of CMT93 cells was trypsinized in 10 mls until the cells detached. Trypsin was inactivated by addition of 30 mls of DMEM (10% FCS, pen/strep) and the cells thoroughly mixed by pipetting. From this solution, 8 mls was transferred into a sterile 50 ml tube and 32 mLs of DMEM 10% added. Cells were well mixed and 250 uLs added to each well of a 48 well plate and incubated overnight at 37 C resulting in adherent cells that were approximately 70% confluent the following morning. The next day, siRNA transfection complexes were created by the following method:
Sequences were ordered from Qiagen as pre-annealed siRNA duplexes. Each well was resuspended in 250 ul of siRNA buffer (from Qiagen) to give a stock concentration of 20 uM. The plate was then placed in a water bath at 95 C for 5 minutes and then allowed to slowly cool to resuspend the duplexes and break apart aggregates. The suspended duplexes were then used in transfection experiments described in standard protocols. The formulation is per well of a 48 well plate containing 250 uL of media; each screen was performed in biological triplicate so the solution was made for 4 wells; 3 for transfection and 1 extra.
0.3 uL of the appropriate siRNA (from a 20 uM stock solution) to 47 uL of serum/antibiotic free media and mixed. To this solution was added 3 uL of Lipofectamine RNAiMAX transfection reagent (Invitrogen) followed by brief vortexing and incubation at room temperature for 20 minutes. 50 uLs of the complex containing mixture was added to each of 3 wells in a 48 well plate containing CMT93 cells. Transfection was for 24 hours at 37 C at which time the cells were washed and RNA isolated for qRT-PCR according the Qiagen Quantitech method (see manufacturer's protocol) for 40 cycles.
FIG. 24 shows the knockdown of IL18 expression with the various siRNA sequences in CMT93 cells post 24 hr transfection. The siRNA sequences tested are listed below:
















SEQ ID

SEQ ID


Well
siRNA sense 5′→3′
NO:
siRNA antisense 5′→3′
NO:







B2
AGGAAAUGAUGUUUAUUGAtt
471
UCAAUAAACAUCAUUUCCUtg
472


 


B3
GGCCGACUUCACUGUACAAtt
473
UUGUACAGUGAAGUCGGCCaa
474


 


B4
GAUGGAGUUUGAAUCUUCAtt
475
UGAAGAUUCAAACUCCAUCtt
476


 


B5
CAACCGCAGUAAUACGGAAtt
477
UUCCGUAUUACUGCGGUUGta
478


 


B6
CGAGGCUGCAUGAUUUAUAtt
479
UAUAAAUCAUGCAGCCUCGgg
480


 


B7
CCUGUAUUUCCAUAACAGAtt
481
UCUGUUAUGGAAAUACAGGcg
482


 


B8
CAUGUACAAAGACAGUGAAtt
483
UUCACUGUCUUUGUACAUGta
484


 


B9
CGAGGAUAUGACUGAUAUUtt
485
AAUAUCAGUCAUAUCCUCGaa
486


 


B10
GGAUAUGACUGAUAUUGAUtt
487
AUCAAUAUCAGUCAUAUCCtc
488


 


B11
CUAACUUACAUCAAAGUUAtt
489
UAACUUUGAUGUAAGUUAGtg
490


 


B12
CUCACUAACUUACAUCAAAtt
491
UUUGAUGUAAGUUAGUGAGag
492









Example 14
Inhibition of Expression of IL-7 in CMT93 Cells
One confluent T-175 flask of CMT93 cells was trypsinized in 10 mls until the cells detached. Trypsin was inactivated by addition of 30 mls of DMEM (10% FCS, pen/strep) and the cells thoroughly mixed by pipetting. From this solution, 8 mls was transferred into a sterile 50 ml tube and 32 mLs of DMEM 10% added. Cells were well mixed and 250 uLs added to each well of a 48 well plate and incubated overnight at 37 C resulting in adherent cells that were approximately 70% confluent the following morning. The next day, siRNA transfection complexes were created by the following method:
Sequences were ordered from Qiagen as pre-annealed siRNA duplexes. Each well was resuspended in 250 ul of siRNA buffer (from Qiagen) to give a stock concentration of 20 uM. The plate was then placed in a water bath at 95 C for 5 minutes and then allowed to slowly cool to resuspend the duplexes and break apart aggregates. The suspended duplexes were then used in transfection experiments described in standard protocols. The formulation is per well of a 48 well plate containing 250 uL of media; each screen was performed in biological triplicate so the solution was made for 4 wells; 3 for transfection and 1 extra.
0.3 uL of the appropriate siRNA (from a 20 uM stock solution) to 47 uL of serum/antibiotic free media and mixed. To this solution was added 3 uL of Lipofectamine RNAiMAX transfection reagent (Invitrogen) followed by brief vortexing and incubation at room temperature for 20 minutes.
50 uLs of the complex containing mixture was added to each of 3 wells in a 48 well plate containing CMT93 cells. Transfection was for 24 hours at 37 C at which time the cells were washed and RNA isolated for qRT-PCR according the Qiagen Quantitech method (see manufacturer's protocol) for 40 cycles.
FIG. 25 shows the knockdown of IL-7 expression with the various siRNA sequences in CMT93 cells post 24 hr transfection. The siRNA sequences tested are listed below:
















SEQ ID

SEQ ID


Well
siRNA sense 5′→3′
NO:
siRNA antisense 5′→3′
NO:







A1
GAUCCUACGGAAGUUAUGGtt
493
CCAUAACUUCCGUAGGAUCcg
494


 


B1
CCAUGUUCCAUGUUUCUUUtt
495
AAAGAAACAUGGAACAUGGtc
496


 


A2
CCUCCCGCAGACCAUGUUCtt
497
GAACAUGGUCUGCGGGAGGcg
498


 


A3
CUCCCGCAGACCAUGUUCCtt
499
GGAACAUGGUCUGCGGGAGgc
500


 


A4
UCCCGCAGACCAUGUUCCAtt
501
UGGAACAUGGUCUGCGGGAgg
502


 


A5
cCCGCAGACCAUGUUCCAUtt
503
AUGGAACAUGGUCUGCGGGag
504


 


A6
CCGCAGACCAUGUUCCAUGtt
505
CAUGGAACAUGGUCUGCGGga
506


 


A7
CGCAGACCAUGUUCCAUGUtt
507
ACAUGGAACAUGGUCUGCGgg
508


 


A10
AGACCAUGUUCCAUGUUUCtt
509
GAAACAUGGAACAUGGUCUgc
510


 


A12
ACCAUGUUCCAUGUUUCUUtt
511
AAGAAACAUGGAACAUGGUct
512









Example 15
Inhibition of Expression of Chitinase3-like-1 (CH13L1) Expression in CMT93 Cells
In a 1.7 ml microcentrifuge tube, 2.4 μl of 20 μM double-stranded RNA solution (from Qiagen) was diluted into 394 μL Opti-MEM serum-free medium (Invitrogen) containing 1 μl Lipofectamine RNAiMAX (Invitrogen), mixed, and incubated 10 min at room temperature to enable the formation of transfection complexes. 100 μl of this mixture was added to each of three wells of a 24-well tissue culture dish, on top of which CMT-93 cells were plated in a 500 μl volume, resulting in a final volume of 600 μl per well and a final RNA concentration of 20 nM. After 24 h transfection, 0.1 μg/ml lipopolysaccharide (LPS) (Sigma) was added to each well and cells were incubated for a further 24 h to stimulate CHI3L1 production, after which cells were washed in PBS and harvested for RNA extraction. CMT-93 cells were prepared for transfection as follows. 1 confluent T-175 flask of CMT93 cells was trypsinized in 10 mls until the cells detached. Trypsin was inactivated by addition of 30 mls of DMEM (10% FBS) and the cells thoroughly mixed by pipetting. From this solution, 10 mls was transferred into a sterile 50 ml tube and 40 mLs of DMEM 10% FBS added. Cells were well mixed and 500 μLs added to each well of a 24-well plate. This concentration of cells resulted in approximately 70% confluency after 24 h of growth.
FIG. 26 shows the knockdown of CH13L1 expression with the various siRNA sequences in CMT93 cells post 24 hr transfection. The siRNA sequences tested are listed below:
















SEQ ID

SEQ ID


Well
siRNA sense 5′→3′
NO:
siRNA antisense 5′→3′
NO:







G1
CCACAUCAUCUACAGCUUUtt
513
AAAGCUGUAGAUGAUGUGGgt
514


 


H1
GGUUUGACAGAUACAGCAAtt
515
UUGCUGUAUCUGUCAAACCta
516


 


G2
UCAUCUACAGCUUUGCCAAtt
517
UUGGCAAAGCUGUAGAUGAtg
518


 


H2
ccCUGUUAAGGAAUGCAAAtt
519
UUUGCAUUCCUUAACAGGGtt
520


 


H3
CAAGUAGGCAAAUAUCUUAtt
521
UAAGAUAUUUGCCUACUUGat
522


 


H4
CAGCUUUGUCAGCAGGAAAtt
523
UUUCCUGCUGACAAAGCUGcg
524


 


G5
GGUUCACCAAGGAGGCAGGtt
525
CCUGCCUCCUUGGUGAACCgg
526


 


H5
GGAUCAAGUAGGCAAAUAUtt
527
AUAUUUGCCUACUUGAUCCaa
528


 


H6
GAGGGACCAUACUAAUUAUtt
529
AUAAUUAGUAUGGUCCCUCaa
530


 


G7
GGCCGGUUCACCAAGGAGGtt
531
CCUCCUUGGUGAACCGGCCtg
532


 


H7
GGACAAGGAGAGUGUCAAAtt
533
UUUGACACUCUCCUUGUCCtc
534


 


G8
CCGGUUCACCAAGGAGGCAtt
535
UGCCUCCUUGGUGAACCGGcc
536


 


H8
CGUACAAGCUGGUCUGCUAtt
537
UAGCAGACCAGCUUGUACGca
538


 


G9
GGAGUUUAAUCUCUUGCAAtt
539
UUGCAAGAGAUUAAACUCCtg
540


 


H9
CAAGGAACUGAAUGCGGAAtt
541
UUCCGCAUUCAGUUCCUUGat
542


 


G10
CCCUGAUCAAGGAACUGAAtt
543
UUCAGUUCCUUGAUCAGGGtg
544


 


H10
CUUGGAUCAAGUAGGCAAAtt
545
UUUGCCUACUUGAUCCAAGtg
546


 


G11
GGAUUGAGGGACCAUACUAtt
547
UAGUAUGGUCCCUCAAUCCtg
548


 


G12
GCAAAUUCUCAGACUCUAAtt
549
UUAGAGUCUGAGAAUUUGCat
550


 


H12
ccuuccCUUAGGAACUUAAtt
551
UUAAGUUCCUAAGGGAAGGat
552









Example 16
Construction of CEQ200
CEQ200 has the following genotype: glnV44(AS), LAM−, rfbC1, endA1, spoT1, thi-1, hsdR17, (rk−mk+),creC510 ΔdapA. The MM294 has the following genotype: glnV44(AS), LAM−, rfbC1, endA1, spoT1, thi-1, hsdR17, (rk−mk+),creC510. We purchased the plasmids from CGSC (see Datsenko et al., (2000) Proc. Natl. Acad. Sci. USA 97,6640-6645).
Derivation of CEQ200




Example 17
Construction of CEQ201
CEQ201 has the following genotype: CEQ200 [glnV44(AS), LAM−, rfbC1, endA1, spoT1, thi-1, hsdR17, (rk−mk+),creC510 ΔdapA ΔrecA. The MM294 has the following genotype: glnV44(AS), LAM−, rfbC1, endA1, spoT1, thi-1, hsdR17, (rk−mk+),creC510. We purchased the plasmids from CGSC (see Datsenko et al., (2000) Proc. Natl. Acad. Sci. USA 97,6640-6645).
Derivation of CEQ200




Example 18
Construction of BTPs (CEQ210) by deletion of minC and/or minD Genes from MM294




Example 19
Illustration of the pMBV40, pMBV43 and pMBV44 Plasmids
The pMBV40, pMBV43 and pMBV44 plasmids may be used as final or intermediary plasmid in the tkRNA system and may be constructed as follows: pUC19 digested with restriction enzyme PvuII. Resultant  ̃2.4 kb fragment was ligated with a  ̃200 bp DNA fragment generated by annealing 5 oligonucleotides with each other. The oligonucleotides have the following names and sequences:







OHTOP1:


(SEQ ID NO: 553)


GACTTCATATACCCAAGCTTGGAAAATTTTTTTTAAAAAAGTCTTGACAC


 


TTTATGCTTCCGGCTCGTATAATGGATCCAGGAGTAACAATACAAATGGA


 


OHTOP2:


(SEQ ID NO: 554)


TTCAAGAGATCCATTTGTATTGTTACTCCTTTTTTTTTTTGTCGACGATC


 


CTTAGCGAAAGCTAAGGATTTTTTTTTTACTCGAGCGGATTACTACATAC


 


OHBOT1:


(SEQ ID NO: 555)


GTATGTAGTAATCCGCTCGAGTAAAAAAAAAATCCTTAGCTTTCGCTAAG


 


GATCGTCGACAAAAAAAAAA


 


OHBOT2:


(SEQ ID NO: 556)


AGGAGTAACAATACAAATGGATCTCTTGAATCCATTTGTATTGTTACTCC


 


TGGATCCATT


 


OHBOT3:


(SEQ ID NO: 557)


ATACGAGCCGGAAGCATAAAGTGTCAAGACTTTTTTAAAAAAAATTTTCC


 


AAGCTTGGGTATATGAAGTC










The predicted sequence of pKSII-inv-hly is as follows:









(SEQ ID NO: 558)



CTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAA



 


TAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTT


 


GGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGG


 


CCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCT


 


AAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGA


 


AAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAA


 


TGCGCCGCTACAGGGCGCGTCCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCC


 


TCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTT


 


CCCAGTCACGACGTTGTAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGCGAATTGGAGCT


 


CCACCGCGGTGGCGGCCGCTCTAGAACTAGTGGATCCCCCGGGCTGCAGCTGGGCCGTAAGATCGGCATTTAA


 


TCGCGACAATCCTTTTAAAAAAACAGCGCCGCTCAATTAACCTGAGCGGCGTTGTTCTTCTGGACGTTTGCTA


 


CTTATGGGGCGAGTCTAGGATTGCCGGACTCCCATTCGCGCCCCAAATAATCAGCTCATTAAACTGTTCTTAT


 


TGCTATCTGTTATCTGGTTATATTGACAGCGCACAGAGCGGGAACGCCAAGTATGCAGGCCCTGGTTGCAGTG


 


CGCCTGTGTCCATATTCATGGTTTCAAAATCCGTGCTGGTCTTTTTGACCCAATATTCACCAGATTGCCAATC


 


AGAACTATACGCGGTCAAGCTCCCCCACTCGCCCCACAATGTCCCGTCAGGCGCACGCGTTCCGTTGGTTGCA


 


CGTGAGGATTCAAGAACCGCAGACATATCTGAACCTTGGCATTGTCTGCTGGCCTCGAGACTGGATACCAGCG


 


ATCTGCCGCCATCGTATATCCACCGATTTGGGTAGAACCGATAACTCACCGAATAACTTGGGAATTTTTTACT


 


TTTCGCCGTCACAGCCACTTCGCTATAGGTTTGGTAGGTAATCGTCACCTGACCCTGATCGTTAACCGATACA


 


TTGGGTGTGAATGACGACGACCACTCATACTGAGTATTATTAGCAACATCGTTATCCATCTGTAACTGGAATG


 


TGGCGTTTTTAAAGATCGTTTTCGGGAACCCTTTATCCGTAGCGAAATTTTGCCCGTTAACCAGAATACCGGT


 


CAGCGTAGGTACCGGGAATAGGGATATTTTTTTCTGCAATGTACTCAGTATCAGGGTATCAACCTGCGGCGTG


 


ATTGTGACATCACCGACACTATTCCCAACCACCGTCGCGGTATAGCTATCTGGCTGCTCGGTAATGGGGCTAA


 


TACTCACCGGCACACCGTTTTGAGTAAAACTCAAGCCCTGCATCCCACTGATAAAATGGCCATTCTTATCGAC


 


AGGGACAAAGGATAATGTGGAACTCATCGTGCCATCAGCCAAGATATCCGGTGTGGAGACGGTGAAACTGGAG


 


CGGCCAGCATCTGGAATAGGATCTGCCGTGAAATTAACCGTCACACTCGGCACACTGAACGCAGCCCCATCCA


 


CTTTCACCGTTACTGTTGCTACCCCCAACGTGGTACTGGTCAATGGTGCGCTATAAGTGCCGTCATTGTGATC


 


CGTGATAACGCCCATATTGCCTAAGGTTGTGTCAAAAGCCACATTCGCGCCAGCCTGCGGGTCCCCATAGGTA


 


TCCTTCAACTCCAACGTGATGGTTGAAGCCATTAGACCATCAGCGATGATAGATGTCGGTACCGCAGCCAGAG


 


TGGATTTATCCGCCGCGATAGTACCCTTAACAAAGTGGGTATCAACACTTTGCCGTTGCCCCTCCACTTCTGC


 


TGTGACTACCGTCACGCCATCTGTCGTATTGGTTAATGCAATGCGCGCGACGCCATTTGCATCTGTCTTTTCC


 


GTGATTTTATTCGGTAGCGCACCATTATTGGTGGTTATCACCACCTCCTGCCCGGCTAAGGGTTTCCCCTCAA


 


AATCAGCAACGGTGAACTCAACGGTGATTGCAGTTTTCCCATTAGCCGGTGCGCCATCACCAATGACGGCCGC


 


CGTTAATGTCAACTGAGGCTGCTGAACGGTGACGCTCAATGTGAATGAGTTAGATCGGTTTCCTTGGTGATCA


 


ACCGCGAGCGCACTAAGCGAATAAAAGTTGGCTGTCAGGTCGTCCGTTACCCGACTCACTTGTGCTGTGCGTT


 


TATAAGGCGGTAAAACCAAGTTGAATTGTGTGGTACTCAGTGGTGTTAATGTGCCGCCAGCGGCAATCAGTTC


 


GGCATCACTCCAGACAATTTCCCTTACAGCAGATGCCCCTTGTACTTGTGCGTTCACCTGATAAACCTGACCC


 


GGCAGGCCGGAGATAGTTGCTGGCGATAATGTCAGTTTAACCACCTGCTGTTTCTGATACTCCAACACGATAT


 


TATTGTTACGATCGACAAGGTTATAGCGGCTCTCCGCCAGTAGACGTGTTCCTGCCACCGCTGAAGGGCTAAG


 


TTGCGACTGAAAACTCTCGCCCAGGCGATAGTTCATTTGGAGGTTCCACTGTGTTTCATGCTTACTGCTTTTC


 


CCCATACGCTGATCTACCCCGACAGTGAGTAGAGGCACGGGGGTGTAATTGATCCCGGCAGTCACGGCATAAG


 


GGTTGCGTTGCAGATTATCTTTACCAAATAAAGCAACACGCTCACCGGTGTATTGCTCATACATCAACTTCCC


 


CCCCAGTTGTGGGAGTGCAGGTAAATAAGCATTCGCGCGCAAATCCCCCCCAGTGGCTGGGCGCTCTTTATAG


 


TCGGAGAAATCACGCGACGAGTGCCATCCATTGAGGCGAAAATACCCATTGGCAGCCAACTGTAAATAATCGG


 


TCCAGGCCTCGGCACCAAGACCGATACGGTGGTTGTGGCCGGTCAAATCATTATCATAAAAAGTATTAAGTCC


 


GTACAGCCAACCGTTCTCCAATGTACGTATCCCGACGCCAAGGTTAAGTGTGTTGCGGCTGTCTTTATTGCGA


 


ATACCTAACTGACTAAAAAAGAGGAATGAAGCAGAGTCATACCAAGGAGCCAGCCAATCAAGAGAGCTTTCTT


 


TTAGCGAAAAATTTTTGTCAAAATTCAGATTAACTTGAGCCGTACCGAATCGATTTAACCACTGTTTGATTTC


 


TTGATTAACCGCATCGCCCACCATTGAGTGAGCAACATCAGATGCCCTGCCTGATGCAGCTAACCTGGCCCCG


 


GTGCTTATCATCTTATTCACCGCTTCAGTCTCCTGCTCCTTATTGGCGCGATCTATTATTGCAGCATTTCTTT


 


CTGTATCCGATGCGGAAAAGGGATTGATTGAACTCTCCATTTCATTATTAGGATGGAGATTTTCAAATGCAGA


 


TGAAGAGACAGAATAAGGCTGGACCTGTTGCGGTGCGTTAGCATCATATTTTTCTGAAGCCCCAGCCATGAAC


 


ATTCCACATATCAAAAAGATACAAATAACTATTCGTGAAATAATATTAAATGAAATTATTTTATTAAAATACA


 


TAGACATTCCCGCATTCCTTATCAAGAGAAACTCACTGATTGGCTGGAAAACCATCATAATTTAAATGAAATA


 


AAGCATACCTGTCATACGTCAAACTGCATGTGCGTTGGCTGTGCTCAACAACTTGAGTTATTTGAGGTATAAC


 


TGGCCACAAACGAGCATTTGAAATCACCTTGACCATTAATTAAAGATGCAATAGTTGAAAGTGAAACTTGTTT


 


TCTAATTTAGTAAAGACATTAAGAGGATAGCACTTTTTTAAAAAACCAGACTGGGCAGATTAAAAATATTCAA


 


AATATATAATAAAACAGTCTATACCATACAGCGATAGAATTGATTTATTGTAACTAAGCAGGTGAGAATATCA


 


AAAAAAACAAAAATACAAAATGAACTATTATCATATAAATAATATCAATTAGAATAAGCCCCCTTCATTTGAT


 


GTTGTCAGTTGTCTGCTGCGGTTTTTATTTCTACTTTCAGTCTGAAGTGTTACTCCGCAATATCCGCATTAAT


 


CCTGATGGTTGCCTTGATGACTGCAGGAATTCGATCCCTCCTTTGATTAGTATATTCCTATCTTAAAGTGACT


 


TTTATGTTGAGGCATTAACATTTGTTAACGACGATAAAGGGACAGCAGGACTAGAATAAAGCTATAAAGCAAG


 


CATATAATATTGCGTTTCATCTTTAGAAGCGAATTTCGCCAATATTATAATTATCAAAAGAGAGGGGTGGCAA


 


ACGGTATTTGGCATTATTAGGTTAAAAAATGTAGAAGGAGAGTGAAACCCATGAAAAAAATAATGCTAGTTTT


 


TATTACACTTATATTAGTTAGTCTACCAATTGCGCAACAAACTGAAGCAAAGGATGCATCTGCATTCAATAAA


 


GAAAATTCAATTTCATCCATGGCACCACCAGCATCTCCGCCTGCAAGTCCTAAGACGCCAATCGAAAAGAAAC


 


ACGCGGATGAAATCGATAAGTATATACAAGGATTGGATTACAATAAAAACAATGTATTAGTATACCACGGAGA


 


TGCAGTGACAAATGTGCCGCCAAGAAAAGGTTACAAAGATGGAAATGAATATATTGTTGTGGAGAAAAAGAAG


 


AAATCCATCAATCAAAATAATGCAGACATTCAAGTTGTGAATGCAATTTCGAGCCTAACCTATCCAGGTGCTC


 


TCGTAAAAGCGAATTCGGAATTAGTAGAAAATCAACCAGATGTTCTCCCTGTAAAACGTGATTCATTAACACT


 


CAGCATTGATTTGCCAGGTATGACTAATCAAGACAATAAAATCGTTGTAAAAAATGCCACTAAATCAAACGTT


 


AACAACGCAGTAAATACATTAGTGGAAAGATGGAATGAAAAATATGCTCAAGCTTATCCAAATGTAAGTGCAA


 


AAATTGATTATGATGACGAAATGGCTTACAGTGAATCACAATTAATTGCGAAATTTGGTACAGCATTTAAAGC


 


TGTAAATAATAGCTTGAATGTAAACTTCGGCGCAATCAGTGAAGGGAAAATGCAAGAAGAAGTCATTAGTTTT


 


AAACAAATTTACTATAACGTGAATGTTAATGAACCTACAAGACCTTCCAGATTTTTCGGCAAAGCTGTTACTA


 


AAGAGCAGTTGCAAGCGCTTGGAGTGAATGCAGAAAATCCTCCTGCATATATCTCAAGTGTGGCGTATGGCCG


 


TCAAGTTTATTTGAAATTATCAACTAATTCCCATAGTACTAAAGTAAAAGCTGCTTTTGATGCTGCCGTAAGC


 


GGAAAATCTGTCTCAGGTGATGTAGAACTAACAAATATCATCAAAAATTCTTCCTTCAAAGCCGTAATTTACG


 


GAGGTTCCGCAAAAGATGAAGTTCAAATCATCGACGGCAACCTCGGAGACTTACGCGATATTTTGAAAAAAGG


 


CGCTACTTTTAATCGAGAAACACCAGGAGTTCCCATTGCTTATACAACAAACTTCCTAAAAGACAATGAATTA


 


GCTGTTATTAAAAACAACTCAGAATATATTGAAACAACTTCAAAAGCTTATACAGATGGAAAAATTAACATCG


 


ATCACTCTGGAGGATACGTTGCTCAATTCAACATTTCTTGGGATGAAGTAAATTATGATCCTGAAGGTAACGA


 


AATTGTTCAACATAAAAACTGGAGCGAAAACAATAAAAGCAAGCTAGCTCATTTCACATCGTCCATCTATTTG


 


CCAGGTAACGCGAGAAATATTAATGTTTACGCTAAAGAATGCACTGGTTTAGCTTGGGAATGGTGGAGAACGG


 


TAATTGATGACCGGAACTTACCACTTGTGAAAAATAGAAATATCTCCATCTGGGGCACCACGCTTTATCCGAA


 


ATATAGTAATAAAGTAGATAATCCAATCGAATAATTGTAAAAGTAATAAAAAATTAAGAATAAAACCGCTTAA


 


CACACACGAAAAAATAAGCTTGTTTTGCACTCTTCGTAAATTATTTTGTGAAGAATGTAGAAACAGGCTTATT


 


TTTTAATTTTTTTAGAAGAATTAACAAATGTAAAAGAATATCTGACTGTTTATCCATATAATATAAGCATATC


 


CCAAAGTTTAAGCCACCTATAGTTTCTACTGCAAAACGTATAATTTAGTTCCCACATATACTAAAAAACGTGT


 


CCTTAACTCTCTCTGTCAGATTAGTTGTAGGTGGCTTAAACTTAGTTTTACGAATTAAAAAGGAGCGGTGAAA


 


TGAAAAGTAAACTTATTTGTATCATCATGGTAATAGCTTTTCAGGCTCATTTCACTATGACGGTAAAAGCAGA


 


TTCTGTCGGGGAAGAAAAACTTCAAAATAATACACAAGCCAAAAAGACCCCTGCTGATTTAAAAGCTTATCAA


 


GCTTATCGATACCGTCGACCTCGAGGGGGGGCCCGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGC


 


GCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATA


 


CGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCT


 


CACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGG


 


CGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGA


 


GCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGT


 


GAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCC


 


CCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAG


 


GCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCT


 


TTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCG


 


CTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTT


 


GAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGT


 


ATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTAT


 


CTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCT


 


GGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGA


 


TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAA


 


AAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACT


 


TGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAG


 


TTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGAT


 


ACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGA


 


AGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGC


 


CAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGC


 


TTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGC


 


TCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGC


 


ATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTG


 


AGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGA


 


ACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGAT


 


CCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTG


 


AGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTC


 


TTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTT


 


AGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCAC










As shown in FIG. 27, the pMBV40 (amp selected having H3 hairpin) or pMBV43 and pMBV44 (kan selected having H3 hairpin) plasmids, are followed by the respective sequences.








pMBV40



(SEQ ID NO: 559)



TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAG



 


CGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATG


 


CGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATCGACGGTATCGATAAGCTTGATAAGCTTTTAAATCAGC


 


AGGGGTCTTTTTGGCTTGTGTATTATTTTGAAGTTTTTCTTCCCCGACAGAATCTGCTTTTACCGTCATAGTGAA


 


ATGAGCCTGAAAAGCTATTACCATGATGATACAAATAAGTTTACTTTTCATTTCACCGCTCCTTTTTAATTCGTA


 


AAACTAAGTTTAAGCCACCTACAACTAATCTGACAGAGAGAGTTAAGGACACGTTTTTTAGTATATGTGGGAACT


 


AAATTATACGTTTTGCAGTAGAAACTATAGGTGGCTTAAACTTTGGGATATGCTTATATTATATGGATAAACAGT


 


CAGATATTCTTTTACATTTGTTAATTCTTCTAAAAAAATTAAAAAATAAGCCTGTTTCTACATTCTTCACAAAAT


 


AATTTACGAAGAGTGCAAAACAAGCTTATTTTTTCGTGTGTGTTAAGCGGTTTTATTCTTAATTTTTTATTACTT


 


TTACAATTATTCGATTGGATTATCTACTTTATTACTATATTTCGGATAAAGCGTGGTGCCCCAGATGGAGATATT


 


TCTATTTTTCACAAGTGGTAAGTTCCGGTCATCAATTACCGTTCTCCACCATTCCCAAGCTAAACCAGTGCATTC


 


TTTAGCGTAAACATTAATATTTCTCGCGTTACCTGGCAAATAGATGGACGATGTGAAATGAGCTAGCTTGCTTTT


 


ATTGTTTTCGCTCCAGTTTTTATGTTGAACAATTTCGTTACCTTCAGGATCATAATTTACTTCATCCCAAGAAAT


 


GTTGAATTGAGCAACGTATCCTCCAGAGTGATCGATGTTAATTTTTCCATCTGTATAAGCTTTTGAAGTTGTTTC


 


AATATATTCTGAGTTGTTTTTAATAACAGCTAATTCATTGTCTTTTAGGAAGTTTGTTGTATAAGCAATGGGAAC


 


TCCTGGTGTTTCTCGATTAAAAGTAGCGCCTTTTTTCAAAATATCGCGTAAGTCTCCGAGGTTGCCGTCGATGAT


 


TTGAACTTCATCTTTTGCGGAACCTCCGTAAATTACGGCTTTGAAGGAAGAATTTTTGATGATATTTGTTAGTTC


 


TACATCACCTGAGACAGATTTTCCGCTTACGGCAGCATCAAAAGCAGCTTTTACTTTAGTACTATGGGAATTAGT


 


TGATAATTTCAAATAAACTTGACGGCCATACGCCACACTTGAGATATATGCAGGAGGATTTTCTGCATTCACTCC


 


AAGCGCTTGCAACTGCTCTTTAGTAACAGCTTTGCCGAAAAATCTGGAAGGTCTTGTAGGTTCATTAACATTCAC


 


GTTATAGTAAATTTGTTTAAAACTAATGACTTCTTCTTGCATTTTCCCTTCACTGATTGCGCCGAAGTTTACATT


 


CAAGCTATTATTTACAGCTTTAAATGCTGTACCAAATTTCGCAATTAATTGTGATTCACTGTAAGCCATTTCGTC


 


ATCATAATCAATTTTTGCACTTACATTTGGATAAGCTTGAGCATATTTTTCATTCCATCTTTCCACTAATGTATT


 


TACTGCGTTGTTAACGTTTGATTTAGTGGCATTTTTTACAACGATTTTATTGTCTTGATTAGTCATACCTGGCAA


 


ATCAATGCTGAGTGTTAATGAATCACGTTTTACAGGGAGAACATCTGGTTGATTTTCTACTAATTCCGAATTCGC


 


TTTTACGAGAGCACCTGGATAGGTTAGGCTCGAAATTGCATTCACAACTTGAATGTCTGCATTATTTTGATTGAT


 


GGATTTCTTCTTTTTCTCCACAACAATATATTCATTTCCATCTTTGTAACCTTTTCTTGGCGGCACATTTGTCAC


 


TGCATCTCCGTGGTATACTAATACATTGTTTTTATTGTAATCCAATCCTTGTATATACTTATCGATTTCATCCGC


 


GTGTTTCTTTTCGATTGGCGTCTTAGGACTTGCAGGCGGAGATGCTGGTGGTGCCATGGATGAAATTGAATTTTC


 


TTTATTGAATGCAGATGCATCCTTTGCTTCAGTTTGTTGCGCAATTGGTAGACTAACTAATATAAGTGTAATAAA


 


AACTAGCATTATTTTTTTCATGGGTTTCACTCTCCTTCTACATTTTTTAACCTAATAATGCCAAATACCGTTTGC


 


CACCCCTCTCTTTTGATAATTATAATATTGGCGAAATTCGCTTCTAAAGATGAAACGCAATATTATATGCTTGCT


 


TTATAGCTTTATTCTAGTCCTGCTGTCCCTTTATCGTCGTTAACAAATGTTAATGCCTCAACATAAAAGTCACTT


 


TAAGATAGGAATATACTAATCAAAGGAGGGATCGAATTCCTGCAGTCATCAAGGCAACCATCAGGATTAATGCGG


 


ATATTGCGGAGTAACACTTCAGACTGAAAGTAGAAATAAAAACCGCAGCAGACAACTGACAACATCAAATGAAGG


 


GGGCTTATTCTAATTGATATTATTTATATGATAATAGTTCATTTTGTATTTTTGTTTTTTTTGATATTCTCACCT


 


GCTTAGTTACAATAAATCAATTCTATCGCTGTATGGTATAGACTGTTTTATTATATATTTTGAATATTTTTAATC


 


TGCCCAGTCTGGTTTTTTAAAAAAGTGCTATCCTCTTAATGTCTTTACTAAATTAGAAAACAAGTTTCACTTTCA


 


ACTATTGCATCTTTAATTAATGGTCAAGGTGATTTCAAATGCTCGTTTGTGGCCAGTTATACCTCAAATAACTCA


 


AGTTGTTGAGCACAGCCAACGCACATGCAGTTTGACGTATGACAGGTATGCTTTATTTCATTTAAATTATGATGG


 


TTTTCCAGCCAATCAGTGAGTTTCTCTTGATAAGGAATGCGGGAATGTCTATGTATTTTAATAAAATAATTTCAT


 


TTAATATTATTTCACGAATAGTTATTTGTATCTTTTTGATATGTGGAATGTTCATGGCTGGGGCTTCAGAAAAAT


 


ATGATGCTAACGCACCGCAACAGGTCCAGCCTTATTCTGTCTCTTCATCTGCATTTGAAAATCTCCATCCTAATA


 


ATGAAATGGAGAGTTCAATCAATCCCTTTTCCGCATCGGATACAGAAAGAAATGCTGCAATAATAGATCGCGCCA


 


ATAAGGAGCAGGAGACTGAAGCGGTGAATAAGATGATAAGCACCGGGGCCAGGTTAGCTGCATCAGGCAGGGCAT


 


CTGATGTTGCTCACTCAATGGTGGGCGATGCGGTTAATCAAGAAATCAAACAGTGGTTAAATCGATTCGGTACGG


 


CTCAAGTTAATCTGAATTTTGACAAAAATTTTTCGCTAAAAGAAAGCTCTCTTGATTGGCTGGCTCCTTGGTATG


 


ACTCTGCTTCATTCCTCTTTTTTAGTCAGTTAGGTATTCGCAATAAAGACAGCCGCAACACACTTAACCTTGGCG


 


TCGGGATACGTACATTGGAGAACGGTTGGCTGTACGGACTTAATACTTTTTATGATAATGATTTGACCGGCCACA


 


ACCACCGTATCGGTCTTGGTGCCGAGGCCTGGACCGATTATTTACAGTTGGCTGCCAATGGGTATTTTCGCCTCA


 


ATGGATGGCACTCGTCGCGTGATTTCTCCGACTATAAAGAGGGCCCAGCCACTGGGGGGGATTTGCGCGCGAATG


 


CTTATTTACCTGCACTCCCACAACTGGGGGGGAAGTTGATGTATGAGCAATACACCGGTGAGCGTGTTGCTTTAT


 


TTGGTAAAGATAATCTGCAACGCAACCCTTATGCCGTGACTGCCGGGATCAATTACACCCCCGTGCCTCTACTCA


 


CTGTCGGGGTAGATCAGCGTATGGGGAAAAGCAGTAAGCATGAAACACAGTGGAACCTCCAAATGAACTATCGCC


 


TGGGCGAGAGTTTTCAGTCGCAACTTAGCCCTTCAGCGGTGGCAGGAACACGTCTACTGGCGGAGAGCCGCTATA


 


ACCTTGTCGATCGTAACAATAATATCGTGTTGGAGTATCAGAAACAGCAGGTGGTTAAACTGACATTATCGCCAG


 


CAACTATCTCCGGCCTGCCGGGTCAGGTTTATCAGGTGAACGCACAAGTACAAGGGGCATCTGCTGTAAGGGAAA


 


TTCTCTGGAGTGATGCCGAACTGATTGCCGCTGGCGGCACATTAACACCACTGAGTACCACACAATTCAACTTGG


 


TTTTACCGCCTTATAAACGCACAGCACAAGTGAGTCGGGTAACGGACGACCTGACAGCCAACTTTTATTCGCTTA


 


GTGCGCTCGCGGTTGATCACCAAGGAAACCGATCTAACTCATTCACATTGAGCGTCACCGTTCAGCAGCCTCAGT


 


TGACATTAACGGCGGCCGTCATTGGTGATGGCGCACCGGCTAATGGGAAAACTGCAATCACCGTTGAGTTCACCG


 


TTGCTGATTTTGAGGGGAAACCCTTAGCCGGGCAGGAGGTGGTGATAACCACCAATAATGGTGCGCTACCGAATA


 


AAATCACGGAAAAGACAGATGCAAATGGCGTCGCGCGCATTGCATTAACCAATACGACAGATGGCGTGACGGTAG


 


TCACAGCAGAAGTGGAGGGGCAACGGCAAAGTGTTGATACCCACTTTGTTAAGGGTACTATCGCGGCGGATAAAT


 


CCACTCTGGCTGCGGTACCGACATCTATCATCGCTGATGGTCTAATGGCTTCAACCATCACGTTGGAGTTGAAGG


 


ATACCTATGGGGACCCGCAGGCTGGCGCGAATGTGGCTTTTGACACAACCTTAGGCAATATGGGCGTTATCACGG


 


ATCACAATGACGGCACTTATAGCGCACCATTGACCAGTACCACGTTGGGGGTAGCAACAGTAACGGTGAAAGTGG


 


ATGGGGCTGCGTTCAGTGTGCCGAGTGTGACGGTTAATTTCACGGCAGATCCTATTCCAGATGCTGGCCGCTCCA


 


GTTTCACCGTCTCCACACCGGATATCTTGGCTGATGGCACGATGAGTTCCACATTATCCTTTGTCCCTGTCGATA


 


AGAATGGCCATTTTATCAGTGGGATGCAGGGCTTGAGTTTTACTCAAAACGGTGTGCCGGTGAGTATTAGCCCCA


 


TTACCGAGCAGCCAGATAGCTATACCGCGACGGTGGTTGGGAATAGTGTCGGTGATGTCACAATCACGCCGCAGG


 


TTGATACCCTGATACTGAGTACATTGCAGAAAAAAATATCCCTATTCCCGGTACCTACGCTGACCGGTATTCTGG


 


TTAACGGGCAAAATTTCGCTACGGATAAAGGGTTCCCGAAAACGATCTTTAAAAACGCCACATTCCAGTTACAGA


 


TGGATAACGATGTTGCTAATAATACTCAGTATGAGTGGTCGTCGTCATTCACACCCAATGTATCGGTTAACGATC


 


AGGGTCAGGTGACGATTACCTACCAAACCTATAGCGAAGTGGCTGTGACGGCGAAAAGTAAAAAATTCCCAAGTT


 


ATTCGGTGAGTTATCGGTTCTACCCAAATCGGTGGATATACGATGGCGGCAGATCGCTGGTATCCAGTCTCGAGG


 


CCAGCAGACAATGCCAAGGTTCAGATATGTCTGCGGTTCTTGAATCCTCACGTGCAACCAACGGAACGCGTGCGC


 


CTGACGGGACATTGTGGGGCGAGTGGGGGAGCTTGACCGCGTATAGTTCTGATTGGCAATCTGGTGAATATTGGG


 


TCAAAAAGACCAGCACGGATTTTGAAACCATGAATATGGACACAGGCGCACTGCAACCAGGGCCTGCATACTTGG


 


CGTTCCCGCTCTGTGCGCTGTCAATATAACCAGATAACAGATAGCAATAAGAACAGTTTAATGAGCTGATTATTT


 


GGGGCGCGAATGGGAGTCCGGCAATCCTAGACTCGCCCCATAAGTAGCAAACGTCCAGAAGAACAACGCCGCTCA


 


GGTTAATTGAGCGGCGCTGTTTTTTTAAAAGGATTGTCGCGATTAAATGCCGATCTTACGGCCCAGCTGCAGCCC


 


GGGGGATCTATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGGATCAGGCGCCATTCGGCATTC


 


AGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGGACTTCATATACCCAAGC


 


TTGGAAAATTTTTTTTAAAAAAGTCTTGACACTTTATGCTTCCGGCTCGTATAATGGATCCAGGAGTAACAATAC


 


AAATGGATTCAAGAGATCCATTTGTATTGTTACTCCTTTTTTTTTTTGTCGACGATCCTTAGCGAAAGCTAAGGA


 


TTTTTTTTTTACTCGAGCGGATTACTACATACCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC


 


GTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAG


 


CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCC


 


AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC


 


ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAA


 


GCTCCCTCGTGGGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG


 


TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC


 


ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG


 


ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCT


 


TGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT


 


TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGC


 


AGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA


 


ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAA


 


AATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGG


 


CACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATAC


 


GGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAG


 


CAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTA


 


ATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCA


 


TCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGAT


 


CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGT


 


TATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTG


 


GTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGG


 


ATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAA


 


GGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTT


 


TCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAAT


 


GTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACA


 


TATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCT


 


AAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTC


 


pMBV43


(SEQ ID NO: 560)



TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAG



 


CGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATG


 


CGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATCGACGGTATCGATAAGCTTGATAAGCTTTTAAATCAGC


 


AGGGGTCTTTTTGGCTTGTGTATTATTTTGAAGTTTTTCTTCCCCGACAGAATCTGCTTTTACCGTCATAGTGAA


 


ATGAGCCTGAAAAGCTATTACCATGATGATACAAATAAGTTTACTTTTCATTTCACCGCTCCTTTTTAATTCGTA


 


AAACTAAGTTTAAGCCACCTACAACTAATCTGACAGAGAGAGTTAAGGACACGTTTTTTAGTATATGTGGGAACT


 


AAATTATACGTTTTGCAGTAGAAACTATAGGTGGCTTAAACTTTGGGATATGCTTATATTATATGGATAAACAGT


 


CAGATATTCTTTTACATTTGTTAATTCTTCTAAAAAAATTAAAAAATAAGCCTGTTTCTACATTCTTCACAAAAT


 


AATTTACGAAGAGTGCAAAACAAGCTTATTTTTTCGTGTGTGTTAAGCGGTTTTATTCTTAATTTTTTATTACTT


 


TTACAATTATTCGATTGGATTATCTACTTTATTACTATATTTCGGATAAAGCGTGGTGCCCCAGATGGAGATATT


 


TCTATTTTTCACAAGTGGTAAGTTCCGGTCATCAATTACCGTTCTCCACCATTCCCAAGCTAAACCAGTGCATTC


 


TTTAGCGTAAACATTAATATTTCTCGCGTTACCTGGCAAATAGATGGACGATGTGAAATGAGCTAGCTTGCTTTT


 


ATTGTTTTCGCTCCAGTTTTTATGTTGAACAATTTCGTTACCTTCAGGATCATAATTTACTTCATCCCAAGAAAT


 


GTTGAATTGAGCAACGTATCCTCCAGAGTGATCGATGTTAATTTTTCCATCTGTATAAGCTTTTGAAGTTGTTTC


 


AATATATTCTGAGTTGTTTTTAATAACAGCTAATTCATTGTCTTTTAGGAAGTTTGTTGTATAAGCAATGGGAAC


 


TCCTGGTGTTTCTCGATTAAAAGTAGCGCCTTTTTTCAAAATATCGCGTAAGTCTCCGAGGTTGCCGTCGATGAT


 


TTGAACTTCATCTTTTGCGGAACCTCCGTAAATTACGGCTTTGAAGGAAGAATTTTTGATGATATTTGTTAGTTC


 


TACATCACCTGAGACAGATTTTCCGCTTACGGCAGCATCAAAAGCAGCTTTTACTTTAGTACTATGGGAATTAGT


 


TGATAATTTCAAATAAACTTGACGGCCATACGCCACACTTGAGATATATGCAGGAGGATTTTCTGCATTCACTCC


 


AAGCGCTTGCAACTGCTCTTTAGTAACAGCTTTGCCGAAAAATCTGGAAGGTCTTGTAGGTTCATTAACATTCAC


 


GTTATAGTAAATTTGTTTAAAACTAATGACTTCTTCTTGCATTTTCCCTTCACTGATTGCGCCGAAGTTTACATT


 


CAAGCTATTATTTACAGCTTTAAATGCTGTACCAAATTTCGCAATTAATTGTGATTCACTGTAAGCCATTTCGTC


 


ATCATAATCAATTTTTGCACTTACATTTGGATAAGCTTGAGCATATTTTTCATTCCATCTTTCCACTAATGTATT


 


TACTGCGTTGTTAACGTTTGATTTAGTGGCATTTTTTACAACGATTTTATTGTCTTGATTAGTCATACCTGGCAA


 


ATCAATGCTGAGTGTTAATGAATCACGTTTTACAGGGAGAACATCTGGTTGATTTTCTACTAATTCCGAATTCGC


 


TTTTACGAGAGCACCTGGATAGGTTAGGCTCGAAATTGCATTCACAACTTGAATGTCTGCATTATTTTGATTGAT


 


GGATTTCTTCTTTTTCTCCACAACAATATATTCATTTCCATCTTTGTAACCTTTTCTTGGCGGCACATTTGTCAC


 


TGCATCTCCGTGGTATACTAATACATTGTTTTTATTGTAATCCAATCCTTGTATATACTTATCGATTTCATCCGC


 


GTGTTTCTTTTCGATTGGCGTCTTAGGACTTGCAGGCGGAGATGCTGGTGGTGCCATGGATGAAATTGAATTTTC


 


TTTATTGAATGCAGATGCATCCTTTGCTTCAGTTTGTTGCGCAATTGGTAGACTAACTAATATAAGTGTAATAAA


 


AACTAGCATTATTTTTTTCATGGGTTTCACTCTCCTTCTACATTTTTTAACCTAATAATGCCAAATACCGTTTGC


 


CACCCCTCTCTTTTGATAATTATAATATTGGCGAAATTCGCTTCTAAAGATGAAACGCAATATTATATGCTTGCT


 


TTATAGCTTTATTCTAGTCCTGCTGTCCCTTTATCGTCGTTAACAAATGTTAATGCCTCAACATAAAAGTCACTT


 


TAAGATAGGAATATACTAATCAAAGGAGGGATCGAATTCCTGCAGTCATCAAGGCAACCATCAGGATTAATGCGG


 


ATATTGCGGAGTAACACTTCAGACTGAAAGTAGAAATAAAAACCGCAGCAGACAACTGACAACATCAAATGAAGG


 


GGGCTTATTCTAATTGATATTATTTATATGATAATAGTTCATTTTGTATTTTTGTTTTTTTTGATATTCTCACCT


 


GCTTAGTTACAATAAATCAATTCTATCGCTGTATGGTATAGACTGTTTTATTATATATTTTGAATATTTTTAATC


 


TGCCCAGTCTGGTTTTTTAAAAAAGTGCTATCCTCTTAATGTCTTTACTAAATTAGAAAACAAGTTTCACTTTCA


 


ACTATTGCATCTTTAATTAATGGTCAAGGTGATTTCAAATGCTCGTTTGTGGCCAGTTATACCTCAAATAACTCA


 


AGTTGTTGAGCACAGCCAACGCACATGCAGTTTGACGTATGACAGGTATGCTTTATTTCATTTAAATTATGATGG


 


TTTTCCAGCCAATCAGTGAGTTTCTCTTGATAAGGAATGCGGGAATGTCTATGTATTTTAATAAAATAATTTCAT


 


TTAATATTATTTCACGAATAGTTATTTGTATCTTTTTGATATGTGGAATGTTCATGGCTGGGGCTTCAGAAAAAT


 


ATGATGCTAACGCACCGCAACAGGTCCAGCCTTATTCTGTCTCTTCATCTGCATTTGAAAATCTCCATCCTAATA


 


ATGAAATGGAGAGTTCAATCAATCCCTTTTCCGCATCGGATACAGAAAGAAATGCTGCAATAATAGATCGCGCCA


 


ATAAGGAGCAGGAGACTGAAGCGGTGAATAAGATGATAAGCACCGGGGCCAGGTTAGCTGCATCAGGCAGGGCAT


 


CTGATGTTGCTCACTCAATGGTGGGCGATGCGGTTAATCAAGAAATCAAACAGTGGTTAAATCGATTCGGTACGG


 


CTCAAGTTAATCTGAATTTTGACAAAAATTTTTCGCTAAAAGAAAGCTCTCTTGATTGGCTGGCTCCTTGGTATG


 


ACTCTGCTTCATTCCTCTTTTTTAGTCAGTTAGGTATTCGCAATAAAGACAGCCGCAACACACTTAACCTTGGCG


 


TCGGGATACGTACATTGGAGAACGGTTGGCTGTACGGACTTAATACTTTTTATGATAATGATTTGACCGGCCACA


 


ACCACCGTATCGGTCTTGGTGCCGAGGCCTGGACCGATTATTTACAGTTGGCTGCCAATGGGTATTTTCGCCTCA


 


ATGGATGGCACTCGTCGCGTGATTTCTCCGACTATAAAGAGCGCCCAGCCACTGGGGGGGATTTGCGCGCGAATG


 


CTTATTTACCTGCACTCCCACAACTGGGGGGGAAGTTGATGTATGAGCAATACACCGGTGAGCGTGTTGCTTTAT


 


TTGGTAAAGATAATCTGCAACGCAACCCTTATGCCGTGACTGCCGGGATCAATTACACCCCCGTGCCTCTACTCA


 


CTGTCGGGGTAGATCAGCGTATGGGGAAAAGCAGTAAGCATGAAACACAGTGGAACCTCCAAATGAACTATCGCC


 


TGGGCGAGAGTTTTCAGTCGCAACTTAGCCCTTCAGCGGTGGCAGGAACACGTCTACTGGCGGAGAGCCGCTATA


 


ACCTTGTCGATCGTAACAATAATATCGTGTTGGAGTATCAGAAACAGCAGGTGGTTAAACTGACATTATCGCCAG


 


CAACTATCTCCGGCCTGCCGGGTCAGGTTTATCAGGTGAACGCACAAGTACAAGGGGCATCTGCTGTAAGGGAAA


 


TTGTCTGGAGTGATGCCGAACTGATTGCCGCTGGCGGCACATTAACACCACTGAGTACCACACAATTCAACTTGG


 


TTTTACCGCCTTATAAACGCACAGCACAAGTGAGTCGGGTAACGGACGACCTGACAGCCAACTTTTATTCGCTTA


 


GTGCGCTCGCGGTTGATCACCAAGGAAACCGATCTAACTCATTCACATTGAGCGTCACCGTTCAGCAGCCTCAGT


 


TGACATTAACGGCGGCCGTCATTGGTGATGGCGCACCGGCTAATGGGAAAACTGCAATCACCGTTGAGTTCACCG


 


TTGCTGATTTTGAGGGGAAACCCTTAGCCGGGCAGGAGGTGGTGATAACCACCAATAATGGTGCGCTACCGAATA


 


AAATCACGGAAAAGACAGATGCAAATGGCGTCGCGCGCATTGCATTAACCAATACGACAGATGGCGTGACGGTAG


 


TCACAGCAGAAGTGGAGGGGCAACGGCAAAGTGTTGATACCCACTTTGTTAAGGGTACTATCGCGGCGGATAAAT


 


CCACTCTGGCTGCGGTACCGACATCTATCATCGCTGATGGTCTAATGGCTTCAACCATCACGTTGGAGTTGAAGG


 


ATACCTATGGGGACCCGCAGGCTGGCGCGAATGTGGCTTTTGACACAACCTTAGGCAATATGGGCGTTATCACGG


 


ATCACAATGACGGCACTTATAGCGCACCATTGACCAGTACCACGTTGGGGGTAGCAACAGTAACGGTGAAAGTGG


 


ATGGGGCTGCGTTCAGTGTGCCGAGTGTGACGGTTAATTTCACGGCAGATCCTATTCCAGATGCTGGCCGCTCCA


 


GTTTCACCGTCTCCACACCGGATATCTTGGCTGATGGCACGATGAGTTCCACATTATCCTTTGTCCCTGTCGATA


 


AGAATGGCCATTTTATCAGTGGGATGCAGGGCTTGAGTTTTACTCAAAACGGTGTGCCGGTGAGTATTAGCCCCA


 


TTACCGAGCAGCCAGATAGCTATACCGCGACGGTGGTTGGGAATAGTGTCGGTGATGTCACAATCACGCCGCAGG


 


TTGATACCCTGATACTGAGTACATTGCAGAAAAAAATATCCCTATTCCCGGTACCTACGCTGACCGGTATTCTGG


 


TTAACGGGCAAAATTTCGCTACGGATAAAGGGTTCCCGAAAACGATCTTTAAAAACGCCACATTCCAGTTACAGA


 


TGGATAACGATGTTGCTAATAATACTCAGTATGAGTGGTCGTCGTCATTCACACCCAATGTATCGGTTAACGATC


 


AGGGTCAGGTGACGATTACCTACCAAACCTATAGCGAAGTGGCTGTGACGGCGAAAAGTAAAAAATTCCCAAGTT


 


ATTCGGTGAGTTATCGGTTCTACCCAAATCGGTGGATATACGATGGCGGCAGATCGCTGGTATCCAGTCTCGAGG


 


CCAGCAGACAATGCCAAGGTTCAGATATGTCTGCGGTTCTTGAATCCTCACGTGCAACCAACGGAACGCGTGCGC


 


CTGACGGGACATTGTGGGGCGAGTGGGGGAGCTTGACCGCGTATAGTTCTGATTGGCAATCTGGTGAATATTGGG


 


TCAAAAAGACCAGCACGGATTTTGAAACCATGAATATGGACACAGGCGCACTGCAACCAGGGCCTGCATACTTGG


 


CGTTCCCGCTCTGTGCGCTGTCAATATAACCAGATAACAGATAGCAATAAGAACAGTTTAATGAGCTGATTATTT


 


GGGGCGCGAATGGGAGTCCGGCAATCCTAGACTCGCCCCATAAGTAGCAAACGTCCAGAAGAACAACGCCGCTCA


 


GGTTAATTGAGCGGCGCTGTTTTTTTAAAAGGATTGTCGCGATTAAATGCCGATCTTACGGCCCAGCTGCAGCCC


 


GGGGGATCTATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCATTCGCCATTC


 


AGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGGACTTCATATACCCAAGC


 


TTGGAAAATTTTTTTTAAAAAAGTCTTGACACTTTATGCTTCCGGCTCGTATAATGGATCCAGGAGTAACAATAC


 


AAATGGATTCAAGAGATCCATTTGTATTGTTACTCCTTTTTTTTTTTGTCGACGATCCTTAGCGAAAGCTAAGGA


 


TTTTTTTTTTACTCGAGCGGATTACTACATACCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC


 


GTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAG


 


CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCC


 


AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC


 


ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAA


 


GCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG


 


TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC


 


ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG


 


ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCT


 


TGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT


 


TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGC


 


AGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA


 


ACGAAAACTCACGTTAAGGGATTTTGGTCATGATCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCT


 


TTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATG


 


ATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAA


 


CAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACC


 


GACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCT


 


TGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGAT


 


CTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTT


 


GATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGT


 


CTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCG


 


CGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGC


 


CGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGT


 


GATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCG


 


CAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGACCAAG


 


CGACGCCCAACCTGCCATCACGAGATTTCGATTCCACCGCCGCCTTCTATGAAATCATGACATTAACCTATAAAA


 


ATAGGCGTATCACGAGGCCCTTTCGTC


 


pMBV44


(SEQ ID NO: 561)



TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAG



 


CGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATG


 


CGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATCGACGGTATCGATAAGCTTGATAAGCTTTTAAATCAGC


 


AGGGGTCTTTTTGGCTTGTGTATTATTTTGAAGTTTTTCTTCCCCGACAGAATCTGCTTTTACCGTCATAGTGAA


 


ATGAGCCTGAAAAGCTATTACCATGATGATACAAATAAGTTTACTTTTCATTTCACCGCTCCTTTTTAATTCGTA


 


AAACTAAGTTTAAGCCACCTACAACTAATCTGACAGAGAGAGTTAAGGACACGTTTTTTAGTATATGTGGGAACT


 


AAATTATACGTTTTGCAGTAGAAACTATAGGTGGCTTAAACTTTGGGATATGCTTATATTATATGGATAAACAGT


 


CAGATATTCTTTTACATTTGTTAATTCTTCTAAAAAAATTAAAAAATAAGCCTGTTTCTACATTCTTCACAAAAT


 


AATTTACGAAGAGTGCAAAACAAGCTTATTTTTTCGTGTGTGTTAAGCGGTTTTATTCTTAATTTTTTATTACTT


 


TTACAATTATTCGATTGGATTATCTACTTTATTACTATATTTCGGATAAAGCGTGGTGCCCCAGATGGAGATATT


 


TCTATTTTTCACAAGTGGTAAGTTCCGGTCATCAATTACCGTTCTCCACCATTCCCAAGCTAAACCAGTGCATTC


 


TTTAGCGTAAACATTAATATTTCTCGCGTTACCTGGCAAATAGATGGACGATGTGAAATGAGCTAGCTTGCTTTT


 


ATTGTTTTCGCTCCAGTTTTTATGTTGAACAATTTCGTTACCTTCAGGATCATAATTTACTTCATCCCAAGAAAT


 


GTTGAATTGAGCAACGTATCCTCCAGAGTGATCGATGTTAATTTTTCCATCTGTATAAGCTTTTGAAGTTGTTTC


 


AATATATTCTGAGTTGTTTTTAATAACAGCTAATTCATTGTCTTTTAGGAAGTTTGTTGTATAAGCAATGGGAAC


 


TCCTGGTGTTTCTCGATTAAAAGTAGCGCCTTTTTTCAAAATATCGCGTAAGTCTCCGAGGTTGCCGTCGATGAT


 


TTGAACTTCATCTTTTGCGGAACCTCCGTAAATTACGGCTTTGAAGGAAGAATTTTTGATGATATTTGTTAGTTC


 


TACATCACCTGAGACAGATTTTCCGCTTACGGCAGCATCAAAAGCAGCTTTTACTTTAGTACTATGGGAATTAGT


 


TGATAATTTCAAATAAACTTGACGGCCATACGCCACACTTGAGATATATGCAGGAGGATTTTCTGCATTCACTCC


 


AAGCGCTTGCAACTGCTCTTTAGTAACAGCTTTGCCGAAAAATCTGGAAGGTCTTGTAGGTTCATTAACATTCAC


 


GTTATAGTAAATTTGTTTAAAACTAATGACTTCTTCTTGCATTTTCCCTTCACTGATTGCGCCGAAGTTTACATT


 


CAAGCTATTATTTACAGCTTTAAATGCTGTACCAAATTTCGCAATTAATTGTGATTCACTGTAAGCCATTTCGTC


 


ATCATAATCAATTTTTGCACTTACATTTGGATAAGCTTGAGCATATTTTTCATTCCATCTTTCCACTAATGTATT


 


TACTGCGTTGTTAACGTTTGATTTAGTGGCATTTTTTACAACGATTTTATTGTCTTGATTAGTCATACCTGGCAA


 


ATCAATGCTGAGTGTTAATGAATCACGTTTTACAGGGAGAACATCTGGTTGATTTTCTACTAATTCCGAATTCGC


 


TTTTACGAGAGCACCTGGATAGGTTAGGCTCGAAATTGCATTCACAACTTGAATGTCTGCATTATTTTGATTGAT


 


GGATTTCTTCTTTTTCTCCACAACAATATATTCATTTCCATCTTTGTAACCTTTTCTTGGCGGCACATTTGTCAC


 


TGCATCTCCGTGGTATACTAATACATTGTTTTTATTGTAATCCAATCCTTGTATATACTTATCGATTTCATCCGC


 


GTGTTTCTTTTCGATTGGCGTCTTAGGACTTGCAGGCGGAGATGCTGGTGGTGCCATGGATGAAATTGAATTTTC


 


TTTATTGAATGCAGATGCATCCTTTGCTTCAGTTTGTTGCGCAATTGGTAGACTAACTAATATAAGTGTAATAAA


 


AACTAGCATTATTTTTTTCATGGGTTTCACTCTCCTTCTACATTTTTTAACCTAATAATGCCAAATACCGTTTGC


 


CACCCCTCTCTTTTGATAATTATAATATTGGCGAAATTCGCTTCTAAAGATGAAACGCAATATTATATGCTTGCT


 


TTATAGCTTTATTCTAGTCCTGCTGTCCCTTTATCGTCGTTAACAAATGTTAATGCCTCAACATAAAAGTCACTT


 


TAAGATAGGAATATACTAATCAAAGGAGGGATCGAATTCCTGCAGTCATCAAGGCAACCATCAGGATTAATGCGG


 


ATATTGCGGAGTAACACTTCAGACTGAAAGTAGAAATAAAAACCGCAGCAGACAACTGACAACATCAAATGAAGG


 


GGGCTTATTCTAATTGATATTATTTATATGATAATAGTTCATTTTGTATTTTTGTTTTTTTTGATATTCTCACCT


 


GCTTAGTTACAATAAATCAATTCTATCGCTGTATGGTATAGACTGTTTTATTATATATTTTGAATATTTTTAATC


 


TGCCCAGTCTGGTTTTTTAAAAAAGTGCTATCCTCTTAATGTCTTTACTAAATTAGAAAACAAGTTTCACTTTCA


 


ACTATTGCATCTTTAATTAATGGTCAAGGTGATTTCAAATGCTCGTTTGTGGCCAGTTATACCTCAAATAACTCA


 


AGTTGTTGAGCACAGCCAACGCACATGCAGTTTGACGTATGACAGGTATGCTTTATTTCATTTAAATTATGATGG


 


TTTTCCAGCCAATCAGTGAGTTTCTCTTGATAAGGAATGCGGGAATGTCTATGTATTTTAATAAAATAATTTCAT


 


TTAATATTATTTCACGAATAGTTATTTGTATCTTTTTGATATGTGGAATGTTCATGGCTGGGGCTTCAGAAAAAT


 


ATGATGCTAACGCACCGCAACAGGTCCAGCCTTATTCTGTCTCTTCATCTGCATTTGAAAATCTCCATCCTAATA


 


ATGAAATGGAGAGTTCAATCAATCCCTTTTCCGCATCGGATACAGAAAGAAATGCTGCAATAATAGATCGCGCCA


 


ATAAGGAGCAGGAGACTGAAGCGGTGAATAAGATGATAAGCACCGGGGCCAGGTTAGCTGCATCAGGCAGGGCAT


 


CTGATGTTGCTCACTCAATGGTGGGCGATGCGGTTAATCAAGAAATCAAACAGTGGTTAAATCGATTCGGTACGG


 


CTCAAGTTAATCTGAATTTTGACAAAAATTTTTCGCTAAAAGAAAGCTCTCTTGATTGGCTGGCTCCTTGGTATG


 


ACTCTGCTTCATTCCTCTTTTTTAGTCAGTTAGGTATTCGCAATAAAGACAGCCGCAACACACTTAACCTTGGCG


 


TCGGGATACGTACATTGGAGAACGGTTGGCTGTACGGACTTAATACTTTTTATGATAATGATTTGACCGGCCACA


 


ACCACCGTATCGGTCTTGGTGCCGAGGCCTGGACCGATTATTTACAGTTGGCTGCCAATGGGTATTTTCGCCTCA


 


ATGGATGGCACTCGTCGCGTGATTTCTCCGACTATAAAGAGCGCCCAGCCACTGGGGGGGATTTGCGCGCGAATG


 


CTTATTTACCTGCACTCCCACAACTGGGGGGGAAGTTGATGTATGAGCAATACACCGGTGAGCGTGTTGCTTTAT


 


TTGGTAAAGATAATCTGCAACGCAACCCTTATGCCGTGACTGCCGGGATCAATTACACCCCCGTGCCTCTACTCA


 


CTGTCGGGGTAGATCAGCGTATGGGGAAAAGCAGTAAGCATGAAACACAGTGGAACCTCCAAATGAACTATCGCC


 


TGGGCGAGAGTTTTCAGTCGCAACTTAGCCCTTCAGCGGTGGCAGGAACACGTCTACTGGCGGAGAGCCGCTATA


 


ACCTTGTCGATCGTAACAATAATATCGTGTTGGAGTATCAGAAACAGCAGGTGGTTAAACTGACATTATCGCCAG


 


CAACTATCTCCGGCCTGCCGGGTCAGGTTTATCAGGTGAACGCACAAGTACAAGGGGCATCTGCTGTAAGGGAAA


 


TTGTCTGGAGTGATGCCGAACTGATTGCCGCTGGCGGCACATTAACACCACTGAGTACCACACAATTCAACTTGG


 


TTTTACCGCCTTATAAACGCACAGCACAAGTGAGTCGGGTAACGGACGACCTGACAGCCAACTTTTATTCGCTTA


 


GTGCGCTCGCGGTTGATCACCAAGGAAACCGATCTAACTCATTCACATTGAGCGTCACCGTTCAGCAGCCTCAGT


 


TGACATTAACGGCGGCCGTCATTGGTGATGGCGCACCGGCTAATGGGAAAACTGCAATCACCGTTGAGTTCACCG


 


TTGCTGATTTTGAGGGGAAACCCTTAGCCGGGCAGGAGGTGGTGATAACCACCAATAATGGTGCGCTACCGAATA


 


AAATCACGGAAAAGACAGATGCAAATGGCGTCGCGCGCATTGCATTAACCAATACGACAGATGGCGTGACGGTAG


 


TCACAGCAGAAGTGGAGGGGCAACGGCAAAGTGTTGATACCCACTTTGTTAAGGGTACTATCGCGGCGGATAAAT


 


CCACTCTGGCTGCGGTACCGACATCTATCATCGCTGATGGTCTAATGGCTTCAACCATCACGTTGGAGTTGAAGG


 


ATACCTATGGGGACCCGCAGGCTGGCGCGAATGTGGCTTTTGACACAACCTTAGGCAATATGGGCGTTATCACGG


 


ATCACAATGACGGCACTTATAGCGCACCATTGACCAGTACCACGTTGGGGGTAGCAACAGTAACGGTGAAAGTGG


 


ATGGGGCTGCGTTCAGTGTGCCGAGTGTGACGGTTAATTTCACGGCAGATCCTATTCCAGATGCTGGCCGCTCCA


 


GTTTCACCGTCTCCACACCGGATATCTTGGCTGATGGCACGATGAGTTCCACATTATCCTTTGTCCCTGTCGATA


 


AGAATGGCCATTTTATCAGTGGGATGCAGGGCTTGAGTTTTACTCAAAACGGTGTGCCGGTGAGTATTAGCCCCA


 


TTACCGAGCAGCCAGATAGCTATACCGCGACGGTGGTTGGGAATAGTGTCGGTGATGTCACAATCACGCCGCAGG


 


TTGATACCCTGATACTGAGTACATTGCAGAAAAAAATATCCCTATTCCCGGTACCTACGCTGACCGGTATTCTGG


 


TTAACGGGCAAAATTTCGCTACGGATAAAGGGTTCCCGAAAACGATCTTTAAAAACGCCACATTCCAGTTACAGA


 


TGGATAACGATGTTGCTAATAATACTCAGTATGAGTGGTCGTCGTCATTCACACCCAATGTATCGGTTAACGATC


 


AGGGTCAGGTGACGATTACCTACCAAACCTATAGCGAAGTGGCTGTGACGGCGAAAAGTAAAAAATTCCCAAGTT


 


ATTCGGTGAGTTATCGGTTCTACCCAAATCGGTGGATATACGATGGCGGCAGATCGCTGGTATCCAGTCTCGAGG


 


CCAGCAGACAATGCCAAGGTTCAGATATGTCTGCGGTTCTTGAATCCTCACGTGCAACCAACGGAACGCGTGCGC


 


CTGACGGGACATTGTGGGGCGAGTGGGGGAGCTTGACCGCGTATAGTTCTGATTGGCAATCTGGTGAATATTGGG


 


TCAAAAAGACCAGCACGGATTTTGAAACCATGAATATGGACACAGGCGCACTGCAACCAGGGCCTGCATACTTGG


 


CGTTCCCGCTCTGTGCGCTGTCAATATAACCAGATAACAGATAGCAATAAGAACAGTTTAATGAGCTGATTATTT


 


GGGGCGCGAATGGGAGTCCGGCAATCCTAGACTCGCCCCATAAGTAGCAAACGTCCAGAAGAACAACGCCGCTCA


 


GGTTAATTGAGCGGCGCTGTTTTTTTAAAAGGATTGTCGCGATTAAATGCCGATCTTACGGCCCAGCTGCAGCCC


 


GGGGGATCTATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCATTCGCCATTC


 


AGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGGACTTCATATACCCAAGC


 


TTGGAAAATTTTTTTTAAAAAAGTCTTGACACTTTATGCTTCCGGCTCGTATAATGGATCCAGGAGTAACAATAC


 


AAATGGATTCAAGAGATCCATTTGTATTGTTACTCCTTTTTTTTTTTGTCGACGATCCTTAGCGAAAGCTAAGGA


 


TTTTTTTTTTACTCGAGCGGATTACTACATACCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC


 


GTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAG


 


CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCC


 


AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC


 


ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAA


 


GCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG


 


TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC


 


ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG


 


ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCT


 


TGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT


 


TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGC


 


AGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA


 


ACGAAAACTCACGTTAAGGGATTTTGGTCATGATTTCATAGAAGGCGGCGGTGGAATCGAAATCTCGTGATGGCA


 


GGTTGGGCGTCGCTTGGTCGGTCATTTCGAACCCCAGAGTCCCGCTCAGAAGAACTCGTCAAGAAGGCGATAGAA


 


GGCGATGCGCTGCGAATCGGGAGCGGCGATACCGTAAAGCACGAGGAAGCGGTCAGCCCATTCGCCGCCAAGCTC


 


TTCAGCAATATCACGGGTAGCCAACGCTATGTCCTGATAGCGGTCCGCCACACCCAGCCGGCCACAGTCGATGAA


 


TCCAGAAAAGCGGCCATTTTCCACCATGATATTCGGCAAGCAGGCATCGCCATGGGTCACGACGAGATCCTCGCC


 


GTCGGGCATGCGCGCCTTGAGCCTGGCGAACAGTTCGGCTGGCGCGAGCCCCTGATGCTCTTCGTCCAGATCATC


 


CTGATCGACAAGACCGGCTTCCATCCGAGTACGTGCTCGCTCGATGCGATGTTTCGCTTGGTGGTCGAATGGGCA


 


GGTAGCCGGATCAAGCGTATGCAGCCGCCGCATTGCATCAGCCATGATGGATACTTTCTCGGCAGGAGCAAGGTG


 


AGATGACAGGAGATCCTGCCCCGGCACTTCGCCCAATAGCAGCCAGTCCCTTCCCGCTTCAGTGACAACGTCGAG


 


CACAGCTGCGCAAGGAACGCCCGTCGTGGCCAGCCACGATAGCCGCGCTGCCTCGTCCTGCAGTTCATTCAGGGC


 


ACCGGACAGGTCGGTCTTGACAAAAAGAACCGGGCGCCCCTGCGCTGACAGCCGGAACACGGCGGCATCAGAGCA


 


GCCGATTGTCTGTTGTGCCCAGTCATAGCCGAATAGCCTCTCCACCCAAGCGGCCGGAGAACCTGCGTGCAATCC


 


ATCTTGTTCAATCATGCGAAACGATCCTCATCCTGTCTCTTGATCAGATCTTGATCCCCTGCGCCATCAGATCCT


 


TGGCGGCAAGAAAGCCATCCAGTTTACTTTGCAGGGCTTCCCAACCTTACCAGATCATGACATTAACCTATAAAA


 


ATAGGCGTATCACGAGGCCCTTTCGTC






Example 23
Construction of pNJSZc Plasmid
pNJSZ is a 10.4 kb plasmid that confers the abilities required to induce tkRNAi. It contains two genes, inv and hly, that allows bacteria to invade mammalian cells and to escape from the entry vacuole. Expression of the short hairpin RNA is different between the original Trip plasmid and pNJSZ. In pNJSZ, expression of shRNA is under the control of a constitutive bacterial promoter which allows for continuous expression. This is different from the original Trip plasmid, which has an ITPG inducible promoter, which controls the expression of the shRNA. Moreover, pNJSZ and the original Trip plasmid contain different antibiotic resistant genes. pNJSZ has the kanamycin resistance gene, whereas the original Trip plasmid has the ampicillin resistance gene. pNJSZc was constructed from pNJSZ by removing any regions of pNJSZ that were not required for its maintenance or abilities to induce tkRNAi.
Step 1 as shown in FIG. 28: Removed an extra BamH1 site at 9778 by digesting pNJSZ with both SpeI (9784) and XmaI (9772), T4 DNA polymerase filled-in these two sites and then allowed the plasmid to self ligate, creating pNJSZ ABamH1.
Step 2 as shown in FIG. 29: Removed both an extra SalI site at 972 and the f1 origin of replication by digesting pNJSZ ΔBamH1 with BglI (208) and PmeI (982), T4 DNA polymerase filled-in these two sites and allowed the plasmid to self ligate, creating pNJSZc.
The pNJSZc DNA sequence is as follows:








(SEQ ID NO: 562)



GGCCGCTCGAGCATGCATCTAGAGGGCCCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTT



 


TACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT


 


GGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAAAAACCGCGCCATGGTGT


 


GTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGGAATAGGAACTTCAAGATCCCC


 


CACGCTGCCGCAAGCACTCAGGGCGCAAGGGCTGCTAAAGGAAACGGAACACGTAGAAAGCCAGTCCGCAGAAAC


 


GGTGCTGACCCCGGATGAATGTCAGCTACTGGGCTATCTGGACAAGGGAAAACGCAAGCGCAAAGAGAAAGCAGG


 


TAGCTTGCAGTGGGCTTACATGGCGATAGCTAGACTGGGCGGTTTTATGGACAGCAAGCGAACCGGAATTGCCAG


 


CTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGATCTGAT


 


GGCGCAGGGGATCAAGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACG


 


CAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATG


 


CCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATG


 


AACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTG


 


TCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTC


 


CTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCG


 


ACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGG


 


ACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATC


 


TCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACT


 


GTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCG


 


GCGAGTGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCC


 


TTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACCTGCCATCACG


 


AGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGCCGGCTGGATGAT


 


CCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCACCCCAGCTTCAAAAGCGCTCTGAAGTTCCTATACTT


 


TCTAGAGAATAGGAACTTCGGAATAGGAACTAAGGAGGATATTCATATGGACCATGGCGCGGCATGCAAGCTCGG


 


TATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAC


 


TATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGT


 


TTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGA


 


TAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGG


 


ATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGT


 


TTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATAC


 


TGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCT


 


AATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACC


 


GGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGA


 


ACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGT


 


AAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGT


 


CGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGC


 


CAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCC


 


TGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAG


 


CGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTA


 


ATGCAGCTGGCACGACAGTATCGATAAGCTTGATAAGCTTTTAAATCAGCAGGGGTCTTTTTGGCTTGTGTATTA


 


TTTTGAAGTTTTTCTTCCCCGACAGAATCTGCTTTTACCGTCATAGTGAAATGAGCCTGAAAAGCTATTACCATG


 


ATGATACAAATAAGTTTACTTTTCATTTCACCGCTCCTTTTTAATTCGTAAAACTAAGTTTAAGCCACCTACAAC


 


TAATCTGACAGAGAGAGTTAAGGACACGTTTTTTAGTATATGTGGGAACTAAATTATACGTTTTGCAGTAGAAAC


 


TATAGGTGGCTTAAACTTTGGGATATGCTTATATTATATGGATAAACAGTCAGATATTCTTTTACATTTGTTAAT


 


TCTTCTAAAAAAATTAAAAAATAAGCCTGTTTCTACATTCTTCACAAAATAATTTACGAAGAGTGCAAAACAAGC


 


TTATTTTTTCGTGTGTGTTAAGCGGTTTTATTCTTAATTTTTTATTACTTTTACAATTATTCGATTGGATTATCT


 


ACTTTATTACTATATTTCGGATAAAGCGTGGTGCCCCAGATGGAGATATTTCTATTTTTCACAAGTGGTAAGTTC


 


CGGTCATCAATTACCGTTCTCCACCATTCCCAAGCTAAACCAGTGCATTCTTTAGCGTAAACATTAATATTTCTC


 


GCGTTACCTGGCAAATAGATGGACGATGTGAAATGAGCTAGCTTGCTTTTATTGTTTTCGCTCCAGTTTTTATGT


 


TGAACAATTTCGTTACCTTCAGGATCATAATTTACTTCATCCCAAGAAATGTTGAATTGAGCAACGTATCCTCCA


 


GAGTGATCGATGTTAATTTTTCCATCTGTATAAGCTTTTGAAGTTGTTTCAATATATTCTGAGTTGTTTTTAATA


 


ACAGCTAATTCATTGTCTTTTAGGAAGTTTGTTGTATAAGCAATGGGAACTCCTGGTGTTTCTCGATTAAAAGTA


 


GCGCCTTTTTTCAAAATATCGCGTAAGTCTCCGAGGTTGCCGTCGATGATTTGAACTTCATCTTTTGCGGAACCT


 


CCGTAAATTACGGCTTTGAAGGAAGAATTTTTGATGATATTTGTTAGTTCTACATCACCTGAGACAGATTTTCCG


 


CTTACGGCAGCATCAAAAGCAGCTTTTACTTTAGTACTATGGGAATTAGTTGATAATTTCAAATAAACTTGACGG


 


CCATACGCCACACTTGAGATATATGGAGGAGGATTTTCTGCATTCACTCCAAGCGCTTCCAACTGCTCTTTAGTA


 


ACAGCTTTGCCGAAAAATCTGGAAGGTCTTGTAGGTTCATTAACATTCACGTTATAGTAAATTTGTTTAAAACTA


 


ATGACTTCTTCTTGCATTTTCCCTTCACTGATTGCGCCGAAGTTTACATTCAAGCTATTATTTACAGCTTTAAAT


 


GCTGTACCAAATTTCGCAATTAATTGTGATTCACTGTAAGCCATTTCGTCATCATAATCAATTTTTGCACTTACA


 


TTTGGATAAGCTTGAGCATATTTTTCATTCCATCTTTCCACTAATGTATTTACTGCGTTGTTAACGTTTGATTTA


 


GTGGCATTTTTTACAACGATTTTATTGTCTTGATTAGTCATACCTGGCAAATCAATGCTGAGTGTTAATGAATCA


 


CGTTTTACAGGGAGAACATCTGGTTGATTTTCTACTAATTCCGAATTOGCTTTTACGAGAGCACCTGGATAGGTT


 


AGGCTCGAAATTGCATTCACAACTTGAATGTCTGCATTATTTTGATTGATGGATTTCTTCTTTTTCTCCACAACA


 


ATATATTCATTTCCATCTTTGTAACCTTTTCTTGGCGGCACATTTGTCACTGCATCTCCGTGGTATACTAATACA


 


TTGTTTTTATTGTAATCCAATCCTTGTATATACTTATCGATTTCATCCGCGTGTTTCTTTTCGATTGGCGTCTTA


 


GGACTTGCAGGCGGAGATGCTGGTGGTGCCATGGATGAAATTGAATTTTCTTTATTGAATGCAGATGCATCCTTT


 


GCTTCAGTTTCTTGCCCAATTGGTAGACTAACTAATATAAGTGTAATAAAAACTAGCATTATTTTTTTCATGGGT


 


TTCACTCTCCTTCTACATTTTTTAACCTAATAATGCCAAATACCGTTTGCCACCCCTCTCTTTTGATAATTATAA


 


TATTGGCGAAATTCGCTTCTAAAGATGAAACGCAATATTATATGCTTGCTTTATAGCTTTATTCTAGTCCTGCTG


 


TCCCTTTATCGTCGTTAACAAATGTTAATGCCTCAACATAAAAGTCACTTTAAGATAGGAATATACTAATCAAAG


 


GAGGGATCGAATTCCTGCAGTCATCAAGGCAACCATCAGGATTAATGCGGATATTGCGGAGTAACACTTCAGACT


 


GAAAGTAGAAATAAAAACCGCAGCAGACAACTGACAACATCAAATGAAGGGGGCTTATTCTAATTGATATTATTT


 


ATATGATAATAGTTCATTTTGTATTTTGTTTTTTTGATATTCTCACCTGCTTAGTTACAATAAATCAATTCTATC


 


GCTGTATGGTATAGACTGTTTTATTATATATTTTGAATATTTTTAATCTGCCCAGTCTGGTTTTTTAAAAAAGTG


 


CTATCCTCTTAATGTCTTTACTAAATTAGAAAACAAGTTTCACTTTCAACTATTGCATCTTTAATTAATGGTCAA


 


GGTGATTTCAAATGCTCGTTTGTGGCCAGTTATACCTCAAATAACTCAAGTTGTTGAGCACAGCCAACGCACATG


 


CAGTTTGACGTATGACAGGTATGCTTTATTTCATTTAAATTATGATGGTTTTCCAGCCAATCAGTGAGTTTCTCT


 


TGATAAGGAATCCGGGAATGTCTATGTATTTTAATAAAATAATTTCATTTAATATTATTTCACGAATAGTTATTT


 


GTATCTTTTTGATATGTGGAATGTTCATGGCTGGGGCTTCAGAAAAATATGATGCTAACGCACCGCAACAGGTCC


 


AGCCTTATTCTGTCTCTTCATCTGCATTTGAAAATCTCCATCCTAATAATGAAATGGAGAGTTCAATCAATCCCT


 


TTTCCGCATCGGATACAGAAAGAAATGCTGCAATAATAGATCGCGCCAATAAGGAGCAGGAGACTGAAGCGGTGA


 


ATAAGATGATAAGCACCGGGGCCAGGTTAGCTGCATCAGGCAGGGCATCTGATGTTGCTCACTCAATGGTGGGCG


 


ATGCGGTTAATCAAGAAATCAAACAGTGGTTAAATCGATTCGGTACGGCTCAAGTTAATCTGAATTTTGACAAAA


 


ATTTTTCGCTAAAAGAAAGCTCTCTTGATTGGCTGGCTCCTTGGTATGACTCTGCTTCATTCCTCTTTTTTAGTC


 


AGTTAGGTATTCGCAATAAAGACAGCCGCAACACACTTAACCTTGGCGTGGGGATACGTACATTGGAGAACGGTT


 


GGCTGTACGGACTTAATACTTTTTATGATAATGATTTGACCGGCCACAACCACCGTATCGGTCTTGGTGCCGAGG


 


CCTGGACCGATTATTTACAGTTGGCTGCCAATGGGTATTTTCGCCTCAATGGATGGCACTCGTCGCGTGATTTCT


 


CCGACTATAAAGAGCGCCCAGCCACTGGGGGGGATTTGCGCGCGAATGCTTATTTACCTGCACTCCCACAACTGG


 


GGGGGAAGTTGATGTATGAGCAATACACCGGTGAGCGTGTTGCTTTATTTGGTAAAGATAATCTGCAACGCAACC


 


CTTATGCCGTGACTGCCGGGATCAATTACACCCCCGTGCCTCTACTCACTGTCGGGGTAGATCAGCGTATGGGGA


 


AAAGCAGTAAGCATGAAACACAGTGGAACCTCCAAATGAACTATCGCCTGGGCGAGAGTTTTCAGTCGCAACTTA


 


GCCCTTCAGCGGTGGCAGGAACACGTCTACTGGCGGAGAGCCGCTATAACCTTGTCGATCGTAACAATAATATCG


 


TGTTGGAGTATCAGAAACAGCAGGTGGTTAAACTGACATTATCGCCAGCAACTATCTCCGGCCTGCCGGGTCAGG


 


TTTATCAGGTGAACGCACAAGTACAAGGGGCATCTGCTGTAAGGGAAATTGTCTGGAGTGATGCCGAACTGATTG


 


CCGCTGGCGGCACATTAACACCACTGAGTACCACACAATTCAACTTGGTTTTACCGCCTTATAAACGCACAGCAC


 


AAGTGAGTCGGGTAACGGACGACCTGACAGCCAACTTTTATTCGCTTAGTGCGCTCGCGGTTGATCACCAAGGAA


 


ACCGATCTAACTCATTCACATTGAGCGTCACCGTTCAGCAGCCTCAGTTGACATTAACGGCGGCCGTCATTGGTG


 


ATGGCGCACCGGCTAATGGGAAAACTGCAATCACCGTTGAGTTCACCGTTGCTGATTTTGAGGGGAAACCCTTAG


 


CCGGGCAGGAGGTGGTGATAACCACCAATAATGGTGCGCTACCGAATAAAATCACGGAAAAGACAGATGCAAATG


 


GCGTCGCGCGCATTGCATTAACCAATACGACAGATGGCGTGACGGTAGTCACAGCAGAAGTGGAGGGGCAACGGC


 


AAAGTGTTGATACCCACTTTGTTAAGGGTACTATCGCGGCGGATAAATCCACTCTGGCTGCGGTACCGACATCTA


 


TCATCGCTGATGGTCTAATGGCTTCAACCATCACGTTGGAGTTGAAGGATACCTATGGGGACCCGCAGGCTGGCG


 


CGAATGTGGCTTTTGACACAACCTTAGGCAATATGGGCGTTATCACGGATCACAATGACGGCACTTATAGCGCAC


 


CATTGACCAGTACCACGTTGGGGGTAGCAACAGTAACGGTGAAAGTGGATGGGGCTGCGTTCAGTGTGCCGAGTG


 


TGACGGTTAATTTCACGGCAGATCCTATTCCAGATGCTGGCCGCTCCAGTTTCACCGTCTCCACACCGGATATCT


 


TGGCTGATGGCACGATGAGTTCCACATTATCCTTTGTCCCTGTCGATAAGAATGGCCATTTTATCAGTGGGATGC


 


AGGGCTTGAGTTTTACTCAAAACGGTGTGCCGGTGAGTATTAGCCCCATTACCGAGCAGCCAGATAGCTATACCG


 


CGACGGTGGTTGGGAATAGTGTCGGTGATGTCACAATCACGCCGCAGGTTGATACCCTGATACTGAGTACATTGC


 


AGAAAAAAATATCCCTATTCCCGGTACCTACGCTGACCGGTATTCTGGTTAACGGGCAAAATTTCGCTACGGATA


 


AAGGGTTCCCGAAAACGATCTTTAAAAACGCCACATTCCAGTTACAGATGGATAACGATGTTGCTAATAATACTC


 


AGTATGAGTGGTCGTCGTCATTCACACCCAATGTATCGGTTAACGATCAGGGTCAGGTGACGATTACCTACCAAA


 


CCTATAGCGAAGTGGCTGTGACGGCGAAAAGTAAAAAATTCCCAAGTTATTCGGTGAGTTATCGGTTCTACCCAA


 


ATCGGTGGATATACGATGGCGGCAGATCGCTGGTATCCAGTCTCGAGGCCAGCAGACAATGCCAAGGTTCAGATA


 


TGTCTGCGGTTCTTGAATCCTCACGTGCAACCAACGGAACGCGTGCGCCTGACGGGACATTGTGGGGCGAGTGGG


 


GGAGCTTGACCGCGTATAGTTCTGATTGGCAATCTGGTGAATATTGGGTCAAAAAGACCAGCACGGATTTTGAAA


 


CCATGAATATGGACACAGGCGCACTGCAACCAGGGCCTGCATACTTGGCGTTCCCGCTCTGTGCGCTGTCAATAT


 


AACCAGATAACAGATAGCAATAAGAACAGTTTAATGAGCTGATTATTTGGGGCGCGAATGGGAGTCCGGCAATCC


 


TAGACTCGCCCCATAAGTAGCAAACGTCCAGAGAACAACGCCGCTCAGGTTAATTGAGCGGCGTTGTTTTTTTAA


 


AAGGATTTGTCGCGATAAGCGTGAGCTGGCGTTAAATGCCGATCTTACGGCCCAGCTGCAGCCCGGCTAGTAACG


 


GCCGCCAGTGTGCTGGAATTCGCCCTTAATCGGCATCATTCACCAAGCTTGCCAGGCGACTGTCTTCAATATTAC


 


AGCCGCAACTACTGACATGGCGGGTGATGGTGTTCACTATTCCAGGGCGATCGGCACCCAACGCAGTGATCACCA


 


GATAATGTTGCGATGACAGTGTCAAACTGGTTATTCCTTCAAGGGGTGAGTTGTTCTTAAGCATGCCGGTTTGCT


 


GTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTG


 


TTTTATTACTCGCTGCCTGTAGTTCTGACTCACGCTATAAGCGTCAGGTCAGTGGTGATGAAGCCTACCTGGAAG


 


CGCCATGGCATGCAAGGGCGAATTCTGCAGATATCCATCACACTGGCGGCCCTAGACCAGGCTTTACACTTTATG


 


CTTCCGGCTCGTATAATGTGTGGAAGGATCCAGGAGTAACAATACAAATGGATTCAAGAGATCCATTTGTATTGT


 


TACTCCTTTGTCGACTGGACAGTTCAAGAGACTGTCCATCAATATCAGCTTTGTCACAAACCCCGCCACCGGCGG


 


GGTTTTTTTCTGCTCTAGGGCCGCTCGAGCATGCATCTAGAGGGCCCAATTCGCCCTATAGTGAGTCGTATTACA


 


ATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCAC


 


ATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGA


 


AAAACCGCGCCATGGTGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGGAAT


 


AGGAACTTCAAGATCCCCCACGCTGCCGCAAGCACTCAGGGCGCAAGGGCTGCTAAAGGAAACGGAACACGTAGA


 


AAGCCAGTCCGCAGAAACGGTGCTGACCCCGGATGAATGTCAGCTACTGGGCTATCTGGACAAGGGAAAACGCAA


 


GCGCAAAGAGAAAGCAGGTAGCTTGCAGTGGGCTTACATGGCGATAGCTAGACTGGGCGGTTTTATGGACAGCAA


 


GCGAACCGGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCT


 


TGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTG


 


AACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGA


 


CAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACC


 


TGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCG


 


CAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCC


 


TGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATC


 


CGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTG


 


TCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCA


 


TGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCT


 


TTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATA


 


TTGCTGAAGAGCTTGGCGGCGAGTGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGC


 


GCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGACCAAGCGAC


 


GCCCAACCTGCCATCACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCG


 


GGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCACCCCAGCTTCAAAAGCGC


 


TCTGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGGAATAGGAACTAAGGAGGATATTCATATGGACCATGG


 


CGCGGCATGCAAGCTCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACAC


 


GACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTG


 


GTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTA


 


GGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCC


 


CGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACC


 


ACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAG


 


AGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCC


 


TACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGA


 


CTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGA


 


GCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAA


 


GGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTG


 


GTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCG


 


GAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT


 


CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAG


 


CCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC


 


GCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGTATCGATAAGCTTGATAAGCTTTTAAATCAGCAGGGGTC


 


TTTTTGGCTTGTGTATTATTTTGAAGTTTTTCTTCCCCGACAGAATCTGCTTTTACCGTCATAGTGAAATGAGCC


 


TGAAAAGCTATTACCATGATGATACAAATAAGTTTACTTTTCATTTCACCGCTCCTTTTTAATTCGTAAAACTAA


 


GTTTAAGCCACCTACAACTAATCTGACAGAGAGAGTTAAGGACACGTTTTTTAGTATATGTGGGAACTAAATTAT


 


ACGTTTTGCAGTAGAAACTATAGGTGGCTTAAACTTTGGGATATGCTTATATTATATGGATAAACAGTCAGATAT


 


TCTTTTACATTTGTTAATTCTTCTAAAAAAATTAAAAAATAAGCCTGTTTCTACATTCTTCACAAAATAATTTAC


 


GAAGAGTGCAAAACAAGCTTATTTTTTCGTGTGTGTTAAGCGGTTTTATTCTTAATTTTTTATTACTTTTACAAT


 


TATTCGATTGGATTATCTACTTTATTACTATATTTCGGATAAAGCGTGGTGCCCCAGATGGAGATATTTCTATTT


 


TTCACAAGTGGTAAGTTCCGGTCATCAATTACCGTTCTCCACCATTCCCAAGCTAAACCAGTGCATTCTTTAGCG


 


TAAACATTAATATTTCTCGCGTTACCTGGCAAATAGATGGACGATGTGAAATGAGCTAGCTTGCTTTTATTGTTT


 


TCGCTCCAGTTTTTATGTTGAACAATTTCGTTACCTTCAGGATCATAATTTACTTCATCCCAAGAAATGTTGAAT


 


TGAGCAACGTATCCTCCAGAGTGATCGATGTTAATTTTTCCATCTGTATAAGCTTTTGAAGTTGTTTCAATATAT


 


TCTGAGTTGTTTTTAATAACAGCTAATTCATTGTCTTTTAGGAAGTTTGTTGTATAAGCAATGGGAACTCCTGGT


 


GTTTCTCGATTAAAAGTAGCGCCTTTTTTCAAAATATCGCGTAAGTCTCCGAGGTTGCCGTCGATGATTTGAACT


 


TCATCTTTTGCGGAACCTCCGTAAATTACGGCTTTGAAGGAAGAATTTTTGATGATATTTGTTAGTTCTACATCA


 


CCTGAGACAGATTTTCCGCTTACGGCAGCATCAAAAGCAGCTTTTACTTTAGTACTATGGGAATTAGTTGATAAT


 


TTCAAATAAACTTGACGGCCATACGCCACACTTGAGATATATGCAGGAGGATTTTCTGCATTCACTCCAAGCGCT


 


TGCAACTGCTCTTTAGTAACAGCTTTGCCGAAAAATCTGGAAGGTCTTGTAGGTTCATTAACATTCACGTTATAG


 


TAAATTTGTTTAAAACTAATGACTTCTTCTTGCATTTTCCCTTCACTGATTGCGCCGAAGTTTACATTCAAGCTA


 


TTATTTACAGCTTTAAATGCTGTACCAAATTTCGCAATTAATTGTGATTCACTGTAAGCCATTTCGTCATCATAA


 


TCAATTTTTGCACTTACATTTGGATAAGCTTGAGCATATTTTTCATTCCATCTTTCCACTAATGTATTTACTGCG


 


TTGTTAACGTTTGATTTAGTGGCATTTTTTACAACGATTTTATTGTCTTGATTAGTCATACCTGGCAAATCAATG


 


CTGAGTGTTAATGAATCACGTTTTACAGGGAGAACATCTGGTTGATTTTCTACTAATTCCGAATTCGCTTTTACG


 


AGAGCACCTGGATAGGTTAGGCTCGAAATTGCATTCACAACTTGAATGTCTGCATTATTTTGATTGATGGATTTC


 


TTCTTTTTCTCCACAACAATATATTCATTTCCATCTTTGTAACCTTTTCTTGGCGGCACATTTGTCACTGCATCT


 


CCGTGGTATACTAATACATTGTTTTTATTGTAATCCAATCCTTGTATATACTTATCGATTTCATCCGCGTGTTTC


 


TTTTCGATTGGCGTCTTAGGACTTGCAGGCGGAGATGCTGGTGGTGCCATGGATGAAATTGAATTTTCTTTATTG


 


AATGCAGATGCATCCTTTGCTTCAGTTTGTTGCGCAATTGGTAGACTAACTAATATAAGTGTAATAAAAACTAGC


 


ATTATTTTTTTCATGGGTTTCACTCTCCTTCTACATTTTTTAACCTAATAATGCCAAATACCGTTTGCCACCCCT


 


CTCTTTTGATAATTATAATATTGGCGAAATTCGCTTCTAAAGATGAAACGCAATATTATATGCTTGCTTTATAGC


 


TTTATTCTAGTCCTGCTGTCCCTTTATCGTCGTTAACAAATGTTAATGCCTCAACATAAAAGTCACTTTAAGATA


 


GGAATATACTAATCAAAGGAGGGATCGAATTCCTGCAGTCATCAAGGCAACCATCAGGATTAATGCGGATATTGC


 


GGAGTAACACTTCAGACTGAAAGTAGAAATAAAAACCGCAGCAGACAACTGACAACATCAAATGAAGGGGGCTTA


 


TTCTAATTGATATTATTTATATGATAATAGTTCATTTTGTATTTTGTTTTTTTGATATTCTCACCTGCTTAGTTA


 


CAATAAATCAATTCTATCGCTGTATGGTATAGACTGTTTTATTATATATTTTGAATATTTTTAATCTGCCCAGTC


 


TGGTTTTTTAAAAAAGTGCTATCCTCTTAATGTCTTTACTAAATTAGAAAACAAGTTTCACTTTCAACTATTGCA


 


TCTTTAATTAATGGTCAAGGTGATTTCAAATGCTCGTTTGTGGCCAGTTATACCTCAAATAACTCAAGTTGTTGA


 


GCACAGCCAACGCACATGCAGTTTGACGTATGACAGGTATGCTTTATTTCATTTAAATTATGATGGTTTTCCAGC


 


CAATCAGTGAGTTTCTCTTGATAAGGAATGCGGGAATGTCTATGTATTTTAATAAAATAATTTCATTTAATATTA


 


TTTCACGAATAGTTATTTGTATCTTTTTGATATGTGGAATGTTCATGGCTGGGGCTTCAGAAAAATATGATGCTA


 


ACGCACCGCAACAGGTCCAGCCTTATTCTGTCTCTTCATCTGCATTTGAAAATCTCCATCCTAATAATGAAATGG


 


AGAGTTCAATCAATCCCTTTTCCGCATCGGATACAGAAAGAAATGCTGCAATAATAGATCGCGCCAATAAGGAGC


 


AGGAGACTGAAGCGGTGAATAAGATGATAAGCACCGGGGCCAGGTTAGCTGCATCAGGCAGGGCATCTGATGTTG


 


CTCACTCAATGGTGGGCGATGCGGTTAATCAAGAAATCAAACAGTGGTTAAATCGATTCGGTACGGCTCAAGTTA


 


ATCTGAATTTTGACAAAAATTTTTCGCTAAAAGAAAGCTCTCTTGATTGGCTGGCTCCTTGGTATGACTCTGCTT


 


CATTCCTCTTTTTTAGTCAGTTAGGTATTCGCAATAAAGACAGCCGCAACACACTTAACCTTGGCGTCGGGATAC


 


GTACATTGGAGAACGGTTGGCTGTACGGACTTAATACTTTTTATGATAATGATTTGACCGGCCACAACCACCGTA


 


TCGGTCTTGGTGCCGAGGCCTGGACCGATTATTTACAGTTGGCTGCCAATGGGTATTTTCGCCTCAATGGATGGC


 


ACTCGTCGCGTGATTTCTCCGACTATAAAGAGCGCCCAGCCACTGGGGGGGATTTGCGCGCGAATGCTTATTTAC


 


CTGCACTCCCACAACTGGGGGGGAAGTTGATGTATGAGCAATACACCGGTGAGCGTGTTGCTTTATTTGGTAAAG


 


ATAATCTGCAACGCAACCCTTATGCCGTGACTGCCGGGATCAATTACACCCCCGTGCCTCTACTCACTGTCGGGG


 


TAGATCAGCGTATGGGGAAAAGCAGTAAGCATGAAACACAGTGGAACCTCCAAATGAACTATCGCCTGGGCGAGA


 


GTTTTCAGTCGCAACTTAGCCCTTCAGCGGTGGCAGGAACACGTCTACTGGCGGAGAGCCGCTATAACCTTGTCG


 


ATCGTAACAATAATATCGTGTTGGAGTATCAGAAACAGCAGGTGGTTAAACTGACATTATCGCCAGCAACTATCT


 


CCGGCCTGCCGGGTCAGGTTTATCAGGTGAACGCACAAGTACAAGGGGCATCTGCTGTAAGGGAAATTGTCTGGA


 


GTGATGCCGAACTGATTGCCGCTGGCGGCACATTAACACCACTGAGTACCACACAATTCAACTTGGTTTTACCGC


 


CTTATAAACGCACAGCACAAGTGAGTCGGGTAACGGACGACCTGACAGCCAACTTTTATTCGCTTAGTGCGCTCG


 


CGGTTGATCACCAAGGAAACCGATCTAACTCATTCACATTGAGCGTCACCGTTCAGCAGCCTCAGTTGACATTAA


 


CGGCGGCCGTCATTGGTGATGGCGCACCGGCTAATGGGAAAACTGCAATCACCGTTGAGTTCACCGTTGCTGATT


 


TTGAGGGGAAACCCTTAGCCGGGCAGGAGGTGGTGATAACCACCAATAATGGTGCGCTACCGAATAAAATCACGG


 


AAAAGACAGATGCAAATGGCGTCGCGCGCATTGCATTAACCAATACGACAGATGGCGTGACGGTAGTCACAGCAG


 


AAGTGGAGGGGCAACGGCAAAGTGTTGATACCCACTTTGTTAAGGGTACTATCGCGGCGGATAAATCCACTCTGG


 


CTGCGGTACCGACATCTATCATCGCTGATGGTCTAATGGCTTCAACCATCACGTTGGAGTTGAAGGATACCTATG


 


GGGACCCGCAGGCTGGCGCGAATGTGGCTTTTGACACAACCTTAGGCAATATGGGCGTTATCACGGATCACAATG


 


ACGGCACTTATAGCGCACCATTGACCAGTACCACGTTGGGGGTAGCAACAGTAACGGTGAAAGTGGATGGGGCTG


 


CGTTCAGTGTGCCGAGTGTGACGGTTAATTTCACGGCAGATCCTATTCCAGATGCTGGCCGCTCCAGTTTCACCG


 


TCTCCACACCGGATATCTTGGCTGATGGCACGATGAGTTCCACATTATCCTTTGTCCCTGTCGATAAGAATGGCC


 


ATTTTATCAGTGGGATGCAGGGCTTGAGTTTTACTCAAAACGGTGTGCCGGTGAGTATTAGCCCCATTACCGAGC


 


AGCCAGATAGCTATACCGCGACGGTGGTTGGGAATAGTGTCGGTGATGTCACAATCACGCCGCAGGTTGATACCC


 


TGATACTGAGTACATTGCAGAAAAAAATATCCCTATTCCCGGTACCTACGCTGACCGGTATTCTGGTTAACGGGC


 


AAAATTTCGCTACGGATAAAGGGTTCCCGAAAACGATCTTTAAAAACGCCACATTCCAGTTACAGATGGATAACG


 


ATGTTGCTAATAATACTCAGTATGAGTGGTCGTCGTCATTCACACCCAATGTATCGGTTAACGATCAGGGTCAGG


 


TGACGATTACCTACCAAACCTATAGCGAAGTGGCTGTGACGGCGAAAAGTAAAAAATTCCCAAGTTATTCGGTGA


 


GTTATCGGTTCTACCCAAATCGGTGGATATACGATGGCGGCAGATCGCTGGTATCCAGTCTCGAGGCCAGCAGAC


 


AATGCCAAGGTTCAGATATGTCTGCGGTTCTTGAATCCTCACGTGCAACCAACGGAACGCGTGCGCCTGACGGGA


 


CATTGTGGGGCGAGTGGGGGAGCTTGACCGCGTATAGTTCTGATTGGCAATCTGGTGAATATTGGGTCAAAAAGA


 


CCAGCACGGATTTTGAAACCATGAATATGGACACAGGCGCACTGCAACCAGGGCCTGCATACTTGGCGTTCCCGC


 


TCTGTGCGCTGTCAATATAACCAGATAACAGATAGCAATAAGAACAGTTTAATGAGCTGATTATTTGGGGCGCGA


 


ATGGGAGTCCGGCAATCCTAGACTCGCCCCATAAGTAGCAAACGTCCAGAGAACAACGCCGCTCAGGTTAATTGA


 


GCGGCGTTGTTTTTTTAAAAGGATTTGTCGCGATAAGCGTGAGCTGGCGTTAAATGCCGATCTTACGGCCCAGCT


 


GCAGCCCGGCTAGTAACGGCCGCCAGTGTGCTGGAATTCGCCCTTAATCGGCATCATTCACCAAGCTTGCCAGGC


 


GACTGTCTTCAATATTACAGCCGCAACTACTGACATGGCGGGTGATGGTGTTCACTATTCCAGGGCGATCGGCAC


 


CCAACGCAGTGATCACCAGATAATGTTGCGATGACAGTGTCAAACTGGTTATTCCTTCAAGGGGTGAGTTGTTCT


 


TAAGCATGCCGGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGG


 


TTGCGGGTGTTTCGCTTGTTTTATTACTCGCTGCCTGTAGTTCTGACTCACGCTATAAGCGTCAGGTCAGTGGTG


 


ATGAAGCCTACCTGGAAGCGCCATGGCATGCAAGGGCGAATTCTGCAGATATCCATCACACTGGCGGCCCTAGAC


 


CAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGAAGGATCCAGGAGTAACAATACAAATGGATTCAA


 


GAGATCCATTTGTATTGTTACTCCTTTGTCGACTGGACAGTTCAAGAGACTGTCCATCAATATCAGCTTTGTCAC


 


AAACCCCGCCACCGGCGGGGTTTTTTTCTGCTCTAG

## Claims

1. An invasive bacterium comprising a prokaryotic vector, said vector comprising one or more DNA molecules encoding one or more siRNAs, at least one Inv gene and at least one HlyA gene, wherein the siRNAs are targeted to a viral gene, wherein at least one of the siRNAs is SEQ ID NOs:384 and 385.

2. A prokaryotic vector comprising at least one DNA molecule encoding one or more siRNAs, at least one Inv gene, at least one HlyA gene, and at least one prokaryotic promoter, wherein the siRNAs are targeted to a viral gene, and wherein at least one of the siRNAs is SEQ ID NOs:384 and 385.

3. The invasive bacterium of claim 1, wherein said invasive bacterium is a non-pathogenic or non-virulent bacterium.

4. The invasive bacterium of claim 1, wherein said invasive bacterium is a therapeutic bacterium.

5. A composition comprising the invasive bacterium of claim 1 and a pharmaceutically acceptable carrier.

6. A eukaryotic host cell comprising the invasive bacterium of claim 1, and a pharmaceutically acceptable carrier.

7. The invasive bacterium of claim 1, wherein said invasive bacterium is attenuated.

8. The prokaryotic vector of claim 2, wherein said prokaryotic promoter is a Ptac promoter.

9. The prokaryotic vector of claim 2, further comprising a rrnC terminator.