# Patent

## Title
Carbazole carboxamide compounds

## Abstract

Disclosed are compounds of Formula (I); and salts thereof, wherein: Formula (II); Q is: R1 is —C(CH3)2OH, —NHC(=0)C(CH3)3, —N(CH3)2, or —CH2Rd; R2 is CI or —CH3; R3 is H, F, or —CH3; Ra is H or —CH3; Rb is H, F, CI, or —OCH3 Rc is H or F; and Rd is —OH, —OCH3, —NHC(=0)CH3, or fORMULA (III), Also disclosed are methods of using such compounds as inhibitors of Bruton's tyrosine kinase (Btk), and pharmaceutical compositions comprising such compounds. These compounds are useful in treating, preventing, or slowing the progression of diseases or disorders in a variety of therapeutic areas, such as autoimmune diseases and vascular disease.

## Background
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a national phase application under 35 U.S.C. §371 of International Patent Application No. PCT/US2014/043980, filed Jun. 25, 2014, which claims priority to U.S. Provisional Application 61/839,130, filed Jun. 25, 2013, which are expressly incorporated fully herein by reference.


DESCRIPTION
The present invention generally relates to carbazole carboxamide compounds useful as kinase inhibitors, including the modulation of Bruton's tyrosine kinase (Btk) and other Tec family kinases such as Itk. Provided herein are carbazole carboxamide compounds, compositions comprising such compounds, and methods of their use. The invention further pertains to pharmaceutical compositions containing at least one compound according to the invention that are useful for the treatment of conditions related to kinase modulation and methods of inhibiting the activity of kinases, including Btk and other Tec family kinases such as Itk, in a mammal.
Protein kinases, the largest family of human enzymes, encompass well over 500 proteins. Btk is a member of the Tec family of tyrosine kinases, and is a regulator of early B-cell development, as well as mature B-cell activation, signaling, and survival.
B-cell signaling through the B-cell receptor (BCR) leads to a wide range of biological outputs, which in turn depend on the developmental stage of the B-cell. The magnitude and duration of BCR signals must be precisely regulated. Aberrant BCR-mediated signaling can cause disregulated B-cell activation and/or the formation of pathogenic auto-antibodies leading to multiple autoimmune and/or inflammatory diseases. Mutation of Btk in humans results in X-linked agammaglobulinaemia (XLA). This disease is associated with the impaired maturation of B-cells, diminished immunoglobulin production, compromised T-cell-independent immune responses and marked attenuation of the sustained calcium signal upon BCR stimulation.
Evidence for the role of Btk in allergic disorders and/or autoimmune disease and/or inflammatory disease has been established in Btk-deficient mouse models. For example, in standard murine preclinical models of systemic lupus erythematosus (SLE), Btk deficiency has been shown to result in a marked amelioration of disease progression. Moreover, Btk deficient mice are also resistant to developing collagen-induced arthritis and are less susceptible to Staphylococcus-induced arthritis.
A large body of evidence supports the role of B-cells and the humoral immune system in the pathogenesis of autoimmune and/or inflammatory diseases. Protein-based therapeutics (such as RITUXAN®) developed to deplete B-cells, represent an important approach to the treatment of a number of autoimmune and/or inflammatory diseases. Because of Btk's role in B-cell activation, inhibitors of Btk can be useful as inhibitors of B-cell mediated pathogenic activity (such as autoantibody production).
Btk is also expressed in mast cells and monocytes and has been shown to be important for the function of these cells. For example, Btk deficiency in mice is associated with impaired IgE-mediated mast cell activation (marked diminution of TNF-alpha and other inflammatory cytokine release), and Btk deficiency in humans is associated with greatly reduced TNF-alpha production by activated monocytes.
Thus, inhibition of Btk activity can be useful for the treatment of allergic disorders and/or autoimmune and/or inflammatory diseases including, but not limited to: SLE, rheumatoid arthritis, multiple vasculitides, idiopathic thrombocytopenic purpura (ITP), myasthenia gravis, allergic rhinitis, multiple sclerosis (MS), transplant rejection, type I diabetes, membranous nephritis, inflammatory bowel disease, autoimmune hemolytic anemia, autoimmune thyroiditis, cold and warm agglutinin diseases, Evans syndrome, hemolytic uremic syndrome/thrombotic thrombocytopenic purpura (HUS/TTP), sarcoidosis, Sjögren's syndrome, peripheral neuropathies (e.g., Guillain-Barre syndrome), pemphigus vulgaris, and asthma.
In addition, Btk has been reported to play a role in controlling B-cell survival in certain B-cell cancers. For example, Btk has been shown to be important for the survival of BCR-Abl-positive B-cell acute lymphoblastic leukemia cells. Thus inhibition of Btk activity can be useful for the treatment of B-cell lymphoma and leukemia.
In view of the numerous conditions that are contemplated to benefit by treatment involving modulation of protein kinases, it is immediately apparent that new compounds capable of modulating protein kinases such as Btk and methods of using these compounds should provide substantial therapeutic benefits to a wide variety of patients.
U.S. Pat. No. 8,084,620 and WO 2011/159857 disclose tricyclic carboxamide compounds useful as kinase inhibitors, including the modulation of Btk and other Tec family kinases.
There still remains a need for compounds useful as Btk inhibitors and yet having selectivity over Jak2 tyrosine kinase. Further, there still remains a need for compounds useful as Btk inhibitors that have selectivity over Jak2 tyrosine kinase and also have improved potency in the whole blood BCR-stimulated CD69 expression assay.
Applicants have found potent compounds that have activity as Btk inhibitors. Further, applicants have found compounds that have activity as Btk inhibitors and are selective over Jak2 tyrosine kinase. Further still, applicants have found compounds that have activity as Btk inhibitors, are selective over Jak2 tyrosine kinase, and have improved potency in the whole blood BCR-stimulated CD69 expression assay. These compounds are provided to be useful as pharmaceuticals with desirable stability, bioavailability, therapeutic index, and toxicity values that are important to their drugability.
SUMMARY OF THE INVENTION
The present invention provides carbazole compounds, which are useful as inhibitors of Btk, and are useful for the treatment of proliferative diseases, allergic diseases, autoimmune diseases and inflammatory diseases, including salts and prodrugs thereof.
The present invention also provides pharmaceutical compositions comprising at least one compound of Formula (I) or a pharmaceutically acceptable salt thereof; and a pharmaceutically acceptable carrier.
The present invention also provides a method of inhibiting Btk activity comprising administering to a mammal in need thereof at least one compound of Formula (I) or a pharmaceutically acceptable salt thereof.
The present invention also provides a method for treating allergic disorders and/or autoimmune and/or inflammatory diseases, comprising administering to a mammal in need thereof at least one compound of Formula (I), or a pharmaceutically acceptable salt thereof.
The present invention also provides a method for treating proliferative diseases, such as cancer, comprising administering to a mammal in need thereof at least one compound of Formula (I) or a pharmaceutically acceptable salt thereof.
The present invention also provides a method of treating a disease or disorder associated with Btk activity, the method comprising administering to a mammal in need thereof, at least one compound of Formula (I) or a pharmaceutically acceptable salt thereof.
The present invention also provides processes and intermediates for making the compounds of Formula (I) and/or salts thereof.
The present invention also provides a compound of Formula (I) or a pharmaceutically acceptable salt thereof, for use in therapy.
The present invention also provides the use of the compounds of Formula (I) or pharmaceutically acceptable salts thereof, for the manufacture of a medicament for the treatment or prophylaxis of Btk related conditions, such as proliferative diseases, allergic diseases, autoimmune diseases and inflammatory diseases.
The present invention also provides the use of the compounds of Formula (I) or pharmaceutically acceptable salts thereof, for the manufacture of a medicament for treatment of cancer.
The compounds of Formula (I) and compositions comprising the compounds of Formula (I) may be used in treating, preventing, or curing various Btk related conditions. Pharmaceutical compositions comprising these compounds are useful in treating, preventing, or slowing the progression of diseases or disorders in a variety of therapeutic areas, such as proliferative diseases, allergic diseases, autoimmune diseases and inflammatory diseases.
These and other features of the invention will be set forth in expanded form as the disclosure continues.



BRIEF DESCRIPTION OF THE DRAWINGS
The invention is illustrated by reference to the accompanying drawings described below.
FIG. 1 shows the absolute stereochemistry of Intermediate 15.
FIG. 2 shows the absolute stereochemistry of the two interconverting diastereomers of Example 3.
FIG. 3 shows the experimental and the simulated PXRD patterns (Cu Kα radiation λ=1.5418 Å) of Example 3 monohydrate, crystal Form H1-6.



DETAILED DESCRIPTION
The first aspect of the present invention provides at least one compound of Formula (I):




or a salt thereof, wherein:





    R1 is —C(CH3)2OH, —NHC(═O)C(CH3)3, —N(CH3)2, or —CH2Rd;
    R2 is Cl or —CH3;
    R3 is H, F, or —CH3;
    Ra is H or —CH3;
    Rb is H, F, Cl, or —OCH3;
    Rc is H or F; and
    Rd is —OH, —OCH3, —NHC(═O)CH3, or






One embodiment provides compounds of Formula (I), wherein R1 is —C(CH3)2OH; and Q, R2, and R3 are defined in the first aspect.
One embodiment provides compounds of Formula (I), wherein R1 is —NHC(═O)C(CH3)3; and Q, R2, and R3 are defined in the first aspect.
One embodiment provides compounds of Formula (I) or a salt thereof, wherein R1 is —N(CH3)2; and Q, R2, and R3 are defined in the first aspect.
One embodiment provides compounds of Formula (I) or a salt thereof, wherein R1 is —CH2Rd; and Q, R2, R3, and Rd are defined in the first aspect.
One embodiment provides compounds of Formula (I) or a salt thereof, wherein R2 is Cl; and Q, R1, and R3 are defined in the first aspect.
One embodiment provides compounds of Formula (I) or a salt thereof, wherein R2 is —CH3; and Q, R1, and R3 are defined in the first aspect.
One embodiment provides compounds of Formula (I), wherein R1 is —C(CH3)2OH; R2 is Cl; and Q and R3 are defined in the first aspect.
One embodiment provides compounds of Formula (I), wherein R1 is —C(CH3)2OH; R2 is Cl; R3 is H; and Q is defined in the first aspect.
One embodiment provides compounds of Formula (I), wherein R1 is —C(CH3)2OH; R2 is —CH3; and Q and R3 are defined in the first aspect.
One embodiment provides compounds of Formula (I), wherein R1 is —C(CH3)2OH; R2 is —CH3; R3 is H; and Q is defined in the first aspect.
One embodiment provides compounds of Formula (I), wherein R1 is —C(CH3)2OH; R2 is —CH3; R3 is —CH3; and Q is defined in the first aspect.
One embodiment provides compounds of Formula (I) or a salt thereof, wherein Q is:




and R1, R2, R3, and Ra are defined in the first aspect. The compounds of this embodiment have the structure of Formula (II):





Included in this embodiment are compounds of Formula (II) in which Q is:





One embodiment provides compounds of Formula (II), wherein R1 is —C(CH3)2OH; and R2, R3, Ra, and Rb are defined in the first aspect. The compounds of this embodiment have the structure of Formula (IIA):




Included in this embodiment are compounds of Formula (IIA) in which R2 is Cl or —CH3; R3 is H; Ra is H or —CH3, including —CD3; and Rb is H, F, or —OCH3. Also, included in this embodiment are compounds of Formula (IIA) in which Q is:





One embodiment provides a compound of Formula (II) or a salt thereof, wherein the compound is selected from: 4-(3-(8-fluoro-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (1 and 2); 4-(3-(S)-(8-fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (3); 4-(3-(S)-(8-fluoro-1-methyl(d3)-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (4); 4-(2-chloro-3-(8-fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)phenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (5); 4-(2-chloro-3-(1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)phenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (6); 7-(2-hydroxypropan-2-yl)-4-(3-(8-methoxy-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-9H-carbazole-1-carboxamide (7); 4-(3-(6-fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (8); 4-(3-(S)-(8-fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-5-methyl-9H-carbazole-1-carboxamide (24); 4-(3-(8-fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-5-methyl-9H-carbazole-1-carboxamide (25); 4-(3-(S)-(8-fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-8-methyl-9H-carbazole-1-carboxamide (26); 4-(3-(S)-(8-fluoro-1-methyl(d3)-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-5-methyl-9H-carbazole-1-carboxamide (27); and 8-fluoro-4-(3-(8-fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (33).
One embodiment provides compounds of Formula (I) or a salt thereof, wherein Q is:




and R1, R2, R3, Rb, and Rc are defined in the first aspect. The compounds of this embodiment have the structure of Formula (III):





Included in this embodiment are compounds of Formula (III) in which Q is:





One embodiment provides compounds of Formula (III) or a salt thereof, wherein R2 is —CH3; and R1, R3, Rb, and Rc are defined in the first aspect. The compounds of this embodiment have the structure of Formula (IIIA):




Included in this embodiment are compounds of Formula (IIIA) in which R1 is —C(CH3)2OH, —NHC(═O)C(CH3)3, —N(CH3)2, or —CH2Rd; R3 is H, F, or —CH3; Rb is H, F, Cl, or —OCH3; Rc is H or F; and Rd is —OH, —OCH3, —NHC(═O)CH3, or





Also included in this embodiment are compounds in which R3 is H.

One embodiment provides a compound of Formula (III) or a salt thereof, wherein the compound is selected from: 7-(2-hydroxypropan-2-yl)-4-(3-(7-methoxy-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-9H-carbazole-1-carboxamide (10); 7-(2-hydroxypropan-2-yl)-4-(3-(6-methoxy-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-9H-carbazole-1-carboxamide (11); 7-(2-hydroxypropan-2-yl)-4-(3-(5-methoxy-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-9H-carbazole-1-carboxamide (12); 4-(3-(5-chloro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (13); 4-(3-(R)-(5-chloro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (14); 4-(3-(S)-(5-chloro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (15); 4-(3-(5-chloro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-pivalamido-9H-carbazole-1-carboxamide (16); 4-(3-(5-chloro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(methoxymethyl)-9H-carbazole-1-carboxamide (17); 4-(3-(5-fluoro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(methoxymethyl)-9H-carbazole-1-carboxamide (18); 4-(3-(4-fluoro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (19); 4-(3-5-fluoro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (21); 4-(3-(5-fluoro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (22 and 23); 4-(3-(5-fluoro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(hydroxymethyl)-9H-carbazole-1-carboxamide (28); 7-(dimethylamino)-4-(3-(5-fluoro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-9H-carbazole-1-carboxamide (29); 7-(acetamidomethyl)-4-(3-(5-fluoro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-9H-carbazole-1-carboxamide (30); and 4-(3-(5-chloro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(pyrrolidin-1-ylmethyl)-9H-carbazole-1-carboxamide (31).
One embodiment provides compounds of Formula (I) or a salt thereof, wherein Q is:




and R1, R2, and R3 are defined in the first aspect. The compounds of this embodiment have the structure of Formula (IV):





One embodiment provides compounds of Formula (IV), wherein R1 is —C(CH3)2OH; and R2, and R3 are defined in the first aspect. The compounds of this embodiment have the structure of Formula (IVA):




Included in this embodiment are compounds of Formula (IVA) in which R2 is —CH3. Also included in this embodiment are compounds of Formula (IVA) in which R3 is H.

One embodiment provides compounds of Formula (I) or a salt thereof, wherein Q is:




The compounds of this embodiment have the structure of Formula (V):





wherein R1, R2, and R3 are defined in the first aspect.

One embodiment provides compounds of Formula (V), wherein R1 is —C(CH3)2OH. The compounds of this embodiment have the structure of Formula (Va):




wherein R2 and R3 are defined in the first aspect. Included in this embodiment are compounds of Formula (Va) in which R2 is —CH3. Also included in this embodiment are compounds of Formula (Va) in which R3 is H.

One embodiment provides compounds of Formula (I) or a salt thereof, wherein R3 is —CH3; and R1, R2, and Q are defined in the first aspect. Included in this embodiment are compounds having the structure of Formula (IA):




Also included in this embodiment are compounds of Formula (I) and compounds of Formula (VI) in which R1 is —C(CH3)2OH.

One embodiment provides a compound of Formula (I) or a salt thereof, wherein the compound is selected from: 4-(3-(8-fluoro-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (1 and 2); 4-(3-(S)-(8-fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (3); 4-(3-(S)-(8-fluoro-1-methyl(d3)-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (4); 4-(2-chloro-3-(8-fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)phenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (5); 4-(2-chloro-3-(1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)phenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (6); 7-(2-hydroxypropan-2-yl)-4-(3-(8-methoxy-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-9H-carbazole-1-carboxamide (7); 4-(3-(6-fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (8); 4-(3-(3-(4-fluorophenyl)-2,6-dioxo-2,3-dihydropyrimidin-1(6H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (9); 7-(2-hydroxypropan-2-yl)-4-(3-(7-methoxy-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-9H-carbazole-1-carboxamide (10); 7-(2-hydroxypropan-2-yl)-4-(3-(6-methoxy-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-9H-carbazole-1-carboxamide (11); 7-(2-hydroxypropan-2-yl)-4-(3-(5-methoxy-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-9H-carbazole-1-carboxamide (12); 4-(3-(5-chloro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (13); 4-(3-(5-chloro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (14 and 15); 4-(3-(5-chloro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-pivalamido-9H-carbazole-1-carboxamide (16); 4-(3-(5-chloro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-pivalamido-9H-carbazole-1-carboxamide (17); 4-(3-(5-fluoro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(methoxymethyl)-9H-carbazole-1-carboxamide (18); 4-(3-(4-fluoro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (19); 4-(3-(5,7-dioxo-5H-thiazolo[3,2-c]pyrimidin-6(7H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (20); 4-(3-(5-fluoro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (21); 4-(3-(5-fluoro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (22 and 23); 4-(3-(S)-(8-fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-5-methyl-9H-carbazole-1-carboxamide (24); 4-(3-(8-fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-5-methyl-9H-carbazole-1-carboxamide (25); 4-(3-(S)-(8-fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-8-methyl-9H-carbazole-1-carboxamide (26); 4-(3-(S)-(8-fluoro-1-methyl(d3)-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-5-methyl-9H-carbazole-1-carboxamide (27); 4-(3-(5-fluoro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(hydroxymethyl)-9H-carbazole-1-carboxamide (28); 7-(dimethylamino)-4-(3-(5-fluoro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-9H-carbazole-1-carboxamide (29); 7-(acetamidomethyl)-4-(3-(5-fluoro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-9H-carbazole-1-carboxamide (30); 4-(3-(5-chloro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(pyrrolidin-1-ylmethyl)-9H-carbazole-1-carboxamide (31); and 4-(3-(5-fluoro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(methoxymethyl)-9H-carbazole-1-carboxamide (32).
Atropisomers are stereoisomers resulting from hindered rotation about a single bond axis where the rotational barrier is high enough to allow for the isolation of the individual rotational isomers. (LaPlante et al., J. Med. Chem., 54:7005-7022 (2011)).
The compounds of Formula (I) have two stereogenic axes: bond (a) between the tricyclic carbazole group and the phenyl group; and bond (b) between the asymmetric heterocyclic dione group Q and the phenyl group.




Due to the non-symmetric nature of the substitutions to the rings connected by the single bonds labeled a and b, and due to limited rotation about these bonds caused by steric hindrance, the compounds of Formula (I) can form rotational isomers. If the rotational energy barriers are sufficiently high, hindered rotations about bond (a) and/or bond (b) occur at rates that are slow enough to allow isolation of the separated atropisomers as different compounds. Thus, the compounds of Formula (I) can form four rotational isomers, which under certain conditions, such as chromatography on a chiral stationary phase, can be separated into individual atropisomers. In solution, the compounds of Formula (I) can be provided as a mixture of four diastereomers, or separated into mixtures of two pairs of diastereomers or single atropisomers.

For the compounds of Formula (I), the pair of rotational isomers formed by hindered rotation about stereogenic axis (a), represented by the structures:




were found to be separable. However, at ambient temperatures and above, a solution containing one of the individual rotational isomers was found to undergo racemization around the stereogenic axis (a) to form a mixture of atropisomers, for example, over a period of hours. Stable rotational isomers formed by hindered rotation about stereogenic axis (b) were isolated and found to be stable in solution at ambient and physiological temperatures.

The two rotational isomers of the compound of Formula (II) that are formed by the hindered rotation about stereogenic axis (b) can be represented as follows:




The two rotational isomers of the compound of Formula (III) that are formed by the hindered rotation about stereogenic axis (b) can be represented as follows:




The two rotational isomers of the compound of Formula (IV) that are formed by the hindered rotation about stereogenic axis (b) can be represented as follows:




The two rotational isomers of the compound of Formula (V) that are formed by the hindered rotation about stereogenic axis (b) can be represented as follows:




The absolute spacial configurations of the atropisomers can be determined by single crystal x-ray crystallography.
Thus, compounds of Formula (I) can be isolated as individual atropisomers, as mixtures comprising the two atropisomers, or as stable pairs of diastereomers, wherein one pair has the (R) configuration about bond (b) but is a mixture of the (R) and (S) configurations about bond (a), and the other pair has the (S) configuration about bond (b) but is a mixture of the (R) and (S) configurations about bond (a).
Compounds of Formula (I) can be separated into pairs of diastereomers with a single absolute configuration about bond (b) but a mixture of two interconverting absolute configurations about bond (a); or alternatively, pairs of diastereomers with a single absolute configuration about bond (a) but a mixture of two interconverting absolute configurations about bond (b). Such separation can be achieved using methods known in the art, such as preparative chromatography on a chiral stationary phase.
Compounds of this invention can exist as a mixture of four atropisomers that can preferably be separated by various techniques, including Supercritical Fluid Chromatography (SFC), to give individual atropisomers or mixtures of two atropisomers. SFC, which is a form of normal phase HPLC, is a separation technique that uses super/subcritical fluid CO2 and polar organic modifiers such as alcohols as mobile phases. (White et al., J. Chromatography A 1074:175-185 (2005)).
One embodiment provides a compound of Formula (II-1) or a salt thereof, wherein R2, R3, Ra, and Rb are defined in the first aspect. Included in this embodiment are compounds of Formula (II-1) in which R2 is Cl or —CH3; R3 is H, F, or —CH3; Ra is H or —CH3, including —CD3; and Rb is H, F, or —OCH3.
One embodiment provides a compound of Formula (III-2) or a salt thereof, wherein R1, R3, Rb, and Rc are defined in the first aspect. Included in this embodiment are compounds of Formula (III-2) in which R1 is —C(CH3)2OH, —NHC(═O)C(CH3)3, —N(CH3)2, or —CH2Rd; R3 is H, F, or —CH3; Rb is H, F, Cl, or —OCH3; Rc is H or F; and Rd is —OH, —OCH3, —NHC(═O)CH3, or




Also included in this embodiment are compounds in which R3 is H.

One embodiment provides a compound selected from the exemplified examples within the scope of the first aspect or a salt thereof.
One embodiment provides a compound selected from any subset list of compounds within the scope of the first aspect or of any of the above embodiments, or a salt thereof.
In one embodiment, a composition is provided comprising a compound of Formula (II) or a salt thereof. Included in this embodiment is a composition comprising a mixture in any proportion of (i) a compound of Formula (II-1) and (ii) a compound of Formula (II-2), or salts thereof. Compositions of this embodiment include pharmaceutical compositions.
In one embodiment, a composition is provided comprising at least 98 equivalent weight % of a compound selected from the compound of Formula (II-1) and the compound of Formula (II-2), based on the total equivalent weight of the compounds of Formula (II-1) and Formula (II-2). Included in this embodiment, are compositions comprising at 99 equivalent weight %, 99.5 equivalent weight %, 99.8 equivalent weight %, and 99.9 equivalent weight % of a selected compound based on the total equivalent weight of the compounds of Formula (II-1) and Formula (II-2). Compositions of this embodiment include pharmaceutical compositions.
In one embodiment, a composition is provided comprising a compound of Formula (III) or a salt thereof. Included in this embodiment is a composition comprising a mixture in any proportion of (i) a compound of Formula (III-1) and (ii) a compound of Formula (III-2), or salts thereof. Compositions of this embodiment include pharmaceutical compositions.
In one embodiment, a composition is provided comprising at least 98 equivalent weight % of a compound selected from the compound of Formula (III-1) and the compound of Formula (III-2), based on the total equivalent weight of the compounds of Formula (III-1) and Formula (III-2). Included in this embodiment, are compositions comprising at 99 equivalent weight %, 99.5 equivalent weight %, 99.8 equivalent weight %, and 99.9 equivalent weight % of a selected compound based on the total equivalent weight of the compounds of Formula (III-1) and Formula (III-2). Compositions of this embodiment include pharmaceutical compositions.
In one embodiment, a composition is provided comprising a compound of Formula (IV) or a salt thereof. Included in this embodiment is a composition comprising a mixture in any proportion of (i) a compound of Formula (IV-1) and (ii) a compound of Formula (IV-2), or salts thereof. Compositions of this embodiment include pharmaceutical compositions.
In one embodiment, a composition is provided comprising at least 98 equivalent weight % of a compound selected from the compound of Formula (IV-1) and the compound of Formula (IV-2), based on the total equivalent weight of the compounds of Formula (IV-1) and Formula (IV-2). Included in this embodiment, are compositions comprising at 99 equivalent weight %, 99.5 equivalent weight %, 99.8 equivalent weight %, and 99.9 equivalent weight % of a selected compound based on the total equivalent weight of the compounds of Formula (IV-1) and Formula (IV-2). Compositions of this embodiment include pharmaceutical compositions.
In one embodiment, a composition is provided comprising a compound of Formula (V) or a salt thereof. Included in this embodiment is a composition comprising a mixture in any proportion of (i) a compound of Formula (V-1) and (ii) a compound of Formula (V-2), or salts thereof. Compositions of this embodiment include pharmaceutical compositions.
In one embodiment, a composition is provided comprising at least 98 equivalent weight % of a compound selected from the compound of Formula (V-1) and the compound of Formula (V-2), based on the total equivalent weight of the compounds of Formula (V-1) and Formula (V-2). Included in this embodiment, are compositions comprising at 99 equivalent weight %, 99.5 equivalent weight %, 99.8 equivalent weight %, and 99.9 equivalent weight % of a selected compound based on the total equivalent weight of the compounds of Formula (V-1) and Formula (V-2). Compositions of this embodiment include pharmaceutical compositions.
The present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. This invention encompasses all combinations of the aspects and/or embodiments of the invention noted herein. It is understood that any and all embodiments of the present invention may be taken in conjunction with any other embodiment or embodiments to describe additional embodiments. It is also to be understood that each individual element of the embodiments is meant to be combined with any and all other elements from any embodiment to describe an additional embodiment.
DEFINITIONS
The features and advantages of the invention may be more readily understood by those of ordinary skill in the art upon reading the following detailed description. It is to be appreciated that certain features of the invention that are, for clarity reasons, described above and below in the context of separate embodiments, may also be combined to form a single embodiment. Conversely, various features of the invention that are, for brevity reasons, described in the context of a single embodiment, may also be combined so as to form sub-combinations thereof. Embodiments identified herein as exemplary or preferred are intended to be illustrative and not limiting.
Unless specifically stated otherwise herein, references made in the singular may also include the plural. For example, “a” and “an” may refer to either one, or one or more.
As used herein, the phase “compounds or a salt thereof” refers to at least one compound, at least one salt of the compounds, or a combination thereof. For example, a compound of Formula (I) or a salt thereof includes a compound of Formula (I); two compounds of Formula (I); a salt of a compound of Formula (I); a compound of Formula (I) and one or more salts of the compound of Formula (I); and two or more salts of a compound of Formula (I).
Unless otherwise indicated, any heteroatom with unsatisfied valences is assumed to have hydrogen atoms sufficient to satisfy the valences.
The definitions set forth herein take precedence over definitions set forth in any patent, patent application, and/or patent application publication incorporated herein by reference.
Listed below are definitions of various terms used to describe the present invention. These definitions apply to the terms as they are used throughout the specification (unless they are otherwise limited in specific instances) either individually or as part of a larger group.
Throughout the specification, groups and substituents thereof may be chosen by one skilled in the field to provide stable moieties and compounds.
In accordance with a convention used in the art,




is used in structural formulas herein to depict the bond that is the point of attachment of the moiety or substituent to the core or backbone structure.

The phrase “pharmaceutically acceptable” is employed herein to refer to those compounds, materials, compositions, and/or dosage forms which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of human beings and animals without excessive toxicity, irritation, allergic response, or other problem or complication, commensurate with a reasonable benefit/risk ratio.
Compounds of Formula (I) can form salts which are also within the scope of this invention. Unless otherwise indicated, reference to an inventive compound is understood to include reference to one or more salts thereof. Pharmaceutically acceptable (i.e., non-toxic, physiologically acceptable) salts are preferred, in which the anion does not contribute significantly to the toxicity or biological activity of the salt. However, other salts may be useful, e.g., in isolation or purification steps which may be employed during preparation, and thus, are contemplated within the scope of the invention. Salts of the compounds of the formula (I) may be formed, for example, by reacting a compound of the Formula (I) with an amount of acid, such as an equivalent amount, in a medium such as one in which the salt precipitates or in an aqueous medium followed by lyophilization.
Exemplary acid addition salts include acetates (such as those formed with acetic acid or trihaloacetic acid, for example, trifluoroacetic acid), adipates, alginates, ascorbates, aspartates, benzoates, benzenesulfonates, bisulfates, borates, butyrates, citrates, camphorates, camphorsulfonates, cyclopentanepropionates, digluconates, dodecylsulfates, ethanesulfonates, fumarates, glucoheptanoates, glycerophosphates, hemisulfates, heptanoates, hexanoates, hydrochlorides (formed with hydrochloric acid), hydrobromides (formed with hydrogen bromide), hydroiodides, maleates (formed with maleic acid), 2-hydroxyethanesulfonates, lactates, methanesulfonates (formed with methanesulfonic acid), 2-naphthalenesulfonates, nicotinates, nitrates, oxalates, pectinates, persulfates, 3-phenylpropionates, phosphates, picrates, pivalates, propionates, salicylates, succinates, sulfates (such as those formed with sulfuric acid), sulfonates (such as those mentioned herein), tartrates, thiocyanates, toluenesulfonates such as tosylates, undecanoates, and the like.
The compounds of Formula (I) can be provided as amorphous solids or crystalline solids. Lyophilization can be employed to provide the compounds of Formula (I) as a solid.
It should further be understood that solvates (e.g., hydrates) of the Compounds of Formula (I) are also within the scope of the present invention. The term “solvate” means a physical association of a compound of Formula (I) with one or more solvent molecules, whether organic or inorganic. This physical association includes hydrogen bonding. In certain instances the solvate will be capable of isolation, for example when one or more solvent molecules are incorporated in the crystal lattice of the crystalline solid. “Solvate” encompasses both solution-phase and isolable solvates. Exemplary solvates include hydrates, ethanolates, methanolates, isopropanolates, acetonitrile solvates, and ethyl acetate solvates. Methods of solvation are known in the art.
Various forms of prodrugs are well known in the art and are described in:
a) Wermuth, C. G. et al., The Practice of Medicinal Chemistry, Chapter 31, Academic Press (1996);
b) Bundgaard, H. ed., Design of Prodrugs, Elsevier (1985);
c) Bundgaard, H., Chapter 5, “Design and Application of Prodrugs”, A Textbook of Drug Design and Development, pp. 113-191, Krogsgaard-Larsen, P. et al., eds., Harwood Academic Publishers (1991); and
d) Testa, B. et al., Hydrolysis in Drug and Prodrug Metabolism, Wiley-VCH (2003).
In addition, compounds of Formula (I), subsequent to their preparation, can be isolated and purified to obtain a composition containing an amount by weight equal to or greater than 99% of a compound of Formula (I) (“substantially pure”), which is then used or formulated as described herein. Such “substantially pure” compounds of Formula (I) are also contemplated herein as part of the present invention.
“Stable compound” and “stable structure” are meant to indicate a compound that is sufficiently robust to survive isolation to a useful degree of purity from a reaction mixture, and formulation into an efficacious therapeutic agent. The present invention is intended to embody stable compounds.
“Therapeutically effective amount” is intended to include an amount of a compound of the present invention alone or an amount of the combination of compounds claimed or an amount of a compound of the present invention in combination with other active ingredients effective to act as an inhibitor to Btk, or effective to treat or prevent autoimmune and/or inflammatory disease states, such as systemic lupus erythematosis, multiple sclerosis and rheumatoid arthritis.
As used herein, “treating” or “treatment” cover the treatment of a disease-state in a mammal, particularly in a human, and include: (a) preventing the disease-state from occurring in a mammal, in particular, when such mammal is predisposed to the disease-state but has not yet been diagnosed as having it; (b) inhibiting the disease-state, i.e., arresting its development; and/or (c) relieving the disease-state, i.e., causing regression of the disease state.
The compounds of the present invention are intended to include all isotopes of atoms occurring in the present compounds. Isotopes include those atoms having the same atomic number but different mass numbers. By way of general example and without limitation, isotopes of hydrogen include deuterium (D) and tritium (T). Isotopes of carbon include 13C and 14C. Isotopically-labeled compounds of the invention can generally be prepared by conventional techniques known to those skilled in the art or by processes analogous to those described herein, using an appropriate isotopically-labeled reagent in place of the non-labeled reagent otherwise employed. For example, methyl (—CH3) also includes deuterated methyl groups such as —CD3.
Compounds in accordance with Formula (I) and/or pharmaceutically acceptable salts thereof can be administered by any means suitable for the condition to be treated, which can depend on the need for site-specific treatment or quantity of Formula (I) compound to be delivered.
Also embraced within this invention is a class of pharmaceutical compositions comprising a compound of Formula (I) and/or pharmaceutically acceptable salts thereof; and one or more non-toxic, pharmaceutically-acceptable carriers and/or diluents and/or adjuvants (collectively referred to herein as “carrier” materials) and, if desired, other active ingredients. The compounds of Formula (I) may be administered by any suitable route, preferably in the form of a pharmaceutical composition adapted to such a route, and in a dose effective for the treatment intended. The compounds and compositions of the present invention may, for example, be administered orally, mucosally, or parentally including intravascularly, intravenously, intraperitoneally, subcutaneously, intramuscularly, and intrastemally in dosage unit formulations containing conventional pharmaceutically acceptable carriers, adjuvants, and vehicles. For example, the pharmaceutical carrier may contain a mixture of mannitol or lactose and microcrystalline cellulose. The mixture may contain additional components such as a lubricating agent, e.g., magnesium stearate and a disintegrating agent such as crospovidone. The carrier mixture may be filled into a gelatin capsule or compressed as a tablet. The pharmaceutical composition may be administered as an oral dosage form or an infusion, for example.
For oral administration, the pharmaceutical composition may be in the form of, for example, a tablet, capsule, liquid capsule, suspension, or liquid. The pharmaceutical composition is preferably made in the form of a dosage unit containing a particular amount of the active ingredient. For example, the pharmaceutical composition may be provided as a tablet or capsule comprising an amount of active ingredient in the range of from about 0.1 to 1000 mg, preferably from about 0.25 to 250 mg, and more preferably from about 0.5 to 100 mg. A suitable daily dose for a human or other mammal may vary widely depending on the condition of the patient and other factors, but, can be determined using routine methods.
Any pharmaceutical composition contemplated herein can, for example, be delivered orally via any acceptable and suitable oral preparations. Exemplary oral preparations, include, but are not limited to, for example, tablets, troches, lozenges, aqueous and oily suspensions, dispersible powders or granules, emulsions, hard and soft capsules, liquid capsules, syrups, and elixirs. Pharmaceutical compositions intended for oral administration can be prepared according to any methods known in the art for manufacturing pharmaceutical compositions intended for oral administration. In order to provide pharmaceutically palatable preparations, a pharmaceutical composition in accordance with the invention can contain at least one agent selected from sweetening agents, flavoring agents, coloring agents, demulcents, antioxidants, and preserving agents.
A tablet can, for example, be prepared by admixing at least one compound of Formula (I) and/or at least one pharmaceutically acceptable salt thereof with at least one non-toxic pharmaceutically acceptable excipient suitable for the manufacture of tablets. Exemplary excipients include, but are not limited to, for example, inert diluents, such as, for example, calcium carbonate, sodium carbonate, lactose, calcium phosphate, and sodium phosphate; granulating and disintegrating agents, such as, for example, microcrystalline cellulose, sodium croscarmellose, corn starch, and alginic acid; binding agents, such as, for example, starch, gelatin, polyvinyl-pyrrolidone, and acacia; and lubricating agents, such as, for example, magnesium stearate, stearic acid, and talc. Additionally, a tablet can either be uncoated, or coated by known techniques to either mask the bad taste of an unpleasant tasting drug, or delay disintegration and absorption of the active ingredient in the gastrointestinal tract thereby sustaining the effects of the active ingredient for a longer period. Exemplary water soluble taste masking materials, include, but are not limited to, hydroxypropyl-methylcellulose and hydroxypropyl-cellulose. Exemplary time delay materials, include, but are not limited to, ethyl cellulose and cellulose acetate butyrate.
Hard gelatin capsules can, for example, be prepared by mixing at least one compound of Formula (I) and/or at least one salt thereof with at least one inert solid diluent, such as, for example, calcium carbonate; calcium phosphate; and kaolin.
Soft gelatin capsules can, for example, be prepared by mixing at least one compound of Formula (I) and/or at least one pharmaceutically acceptable salt thereof with at least one water soluble carrier, such as, for example, polyethylene glycol; and at least one oil medium, such as, for example, peanut oil, liquid paraffin, and olive oil.
An aqueous suspension can be prepared, for example, by admixing at least one compound of Formula (I) and/or at least one pharmaceutically acceptable salt thereof with at least one excipient suitable for the manufacture of an aqueous suspension. Exemplary excipients suitable for the manufacture of an aqueous suspension, include, but are not limited to, for example, suspending agents, such as, for example, sodium carboxymethylcellulose, methylcellulose, hydroxypropylmethyl-cellulose, sodium alginate, alginic acid, polyvinyl-pyrrolidone, gum tragacanth, and gum acacia; dispersing or wetting agents, such as, for example, a naturally-occurring phosphatide, e.g., lecithin; condensation products of alkylene oxide with fatty acids, such as, for example, polyoxyethylene stearate; condensation products of ethylene oxide with long chain aliphatic alcohols, such as, for example heptadecaethylene-oxycetanol; condensation products of ethylene oxide with partial esters derived from fatty acids and hexitol, such as, for example, polyoxyethylene sorbitol monooleate; and condensation products of ethylene oxide with partial esters derived from fatty acids and hexitol anhydrides, such as, for example, polyethylene sorbitan monooleate. An aqueous suspension can also contain at least one preservative, such as, for example, ethyl and n-propyl p-hydroxybenzoate; at least one coloring agent; at least one flavoring agent; and/or at least one sweetening agent, including but not limited to, for example, sucrose, saccharin, and aspartame.
Oily suspensions can, for example, be prepared by suspending at least one compound of Formula (I) and/or at least one pharmaceutically acceptable salt thereof in either a vegetable oil, such as, for example, arachis oil; olive oil; sesame oil; and coconut oil; or in mineral oil, such as, for example, liquid paraffin. An oily suspension can also contain at least one thickening agent, such as, for example, beeswax; hard paraffin; and cetyl alcohol. In order to provide a palatable oily suspension, at least one of the sweetening agents already described hereinabove, and/or at least one flavoring agent can be added to the oily suspension. An oily suspension can further contain at least one preservative, including, but not limited to, for example, an anti-oxidant, such as, for example, butylated hydroxyanisol, and alpha-tocopherol.
Dispersible powders and granules can, for example, be prepared by admixing at least one compound of Formula (I) and/or at least one pharmaceutically acceptable salt thereof with at least one dispersing and/or wetting agent; at least one suspending agent; and/or at least one preservative. Suitable dispersing agents, wetting agents, and suspending agents are as already described above. Exemplary preservatives include, but are not limited to, for example, anti-oxidants, e.g., ascorbic acid. In addition, dispersible powders and granules can also contain at least one excipient, including, but not limited to, for example, sweetening agents; flavoring agents; and coloring agents.
An emulsion of at least one compound of Formula (I) and/or at least one pharmaceutically acceptable salt thereof can, for example, be prepared as an oil-in-water emulsion. The oily phase of the emulsions comprising compounds of Formula (I) may be constituted from known ingredients in a known manner. The oil phase can be provided by, but is not limited to, for example, a vegetable oil, such as, for example, olive oil and arachis oil; a mineral oil, such as, for example, liquid paraffin; and mixtures thereof. While the phase may comprise merely an emulsifier, it may comprise a mixture of at least one emulsifier with a fat or an oil or with both a fat and an oil. Suitable emulsifying agents include, but are not limited to, for example, naturally-occurring phosphatides, e.g., soy bean lecithin; esters or partial esters derived from fatty acids and hexitol anhydrides, such as, for example, sorbitan monooleate; and condensation products of partial esters with ethylene oxide, such as, for example, polyoxyethylene sorbitan monooleate. Preferably, a hydrophilic emulsifier is included together with a lipophilic emulsifier which acts as a stabilizer. It is also preferred to include both an oil and a fat. Together, the emulsifier(s) with or without stabilizer(s) make-up the so-called emulsifying wax, and the wax together with the oil and fat make up the so-called emulsifying ointment base which forms the oily dispersed phase of the cream formulations. An emulsion can also contain a sweetening agent, a flavoring agent, a preservative, and/or an antioxidant. Emulsifiers and emulsion stabilizers suitable for use in the formulation of the present invention include Tween 60, Span 80, cetostearyl alcohol, myristyl alcohol, glyceryl monostearate, sodium lauryl sulfate, glyceryl distearate alone or with a wax, or other materials well known in the art.
The compounds of Formula (I) and/or at least one pharmaceutically acceptable salt thereof can, for example, also be delivered intravenously, subcutaneously, and/or intramuscularly via any pharmaceutically acceptable and suitable injectable form. Exemplary injectable forms include, but are not limited to, for example, sterile aqueous solutions comprising acceptable vehicles and solvents, such as, for example, water, Ringer's solution, and isotonic sodium chloride solution; sterile oil-in-water microemulsions; and aqueous or oleaginous suspensions.
Formulations for parenteral administration may be in the form of aqueous or non-aqueous isotonic sterile injection solutions or suspensions. These solutions and suspensions may be prepared from sterile powders or granules using one or more of the carriers or diluents mentioned for use in the formulations for oral administration or by using other suitable dispersing or wetting agents and suspending agents. The compounds may be dissolved in water, polyethylene glycol, propylene glycol, ethanol, corn oil, cottonseed oil, peanut oil, sesame oil, benzyl alcohol, sodium chloride, tragacanth gum, and/or various buffers. Other adjuvants and modes of administration are well and widely known in the pharmaceutical art. The active ingredient may also be administered by injection as a composition with suitable carriers including saline, dextrose, or water, or with cyclodextrin (i.e., CAPTISOL®), cosolvent solubilization (i.e., propylene glycol) or micellar solubilization (i.e., Tween 80).
The sterile injectable preparation may also be a sterile injectable solution or suspension in a non-toxic parenterally acceptable diluent or solvent, for example as a solution in 1,3-butanediol. Among the acceptable vehicles and solvents that may be employed are water, Ringer's solution, and isotonic sodium chloride solution. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose any bland fixed oil may be employed, including synthetic mono- or diglycerides. In addition, fatty acids such as oleic acid find use in the preparation of injectables.
A sterile injectable oil-in-water microemulsion can, for example, be prepared by 1) dissolving at least one compound of Formula (I) in an oily phase, such as, for example, a mixture of soybean oil and lecithin; 2) combining the Formula (I) containing oil phase with a water and glycerol mixture; and 3) processing the combination to form a microemulsion.
A sterile aqueous or oleaginous suspension can be prepared in accordance with methods already known in the art. For example, a sterile aqueous solution or suspension can be prepared with a non-toxic parenterally-acceptable diluent or solvent, such as, for example, 1,3-butane diol; and a sterile oleaginous suspension can be prepared with a sterile non-toxic acceptable solvent or suspending medium, such as, for example, sterile fixed oils, e.g., synthetic mono- or diglycerides; and fatty acids, such as, for example, oleic acid.
Pharmaceutically acceptable carriers, adjuvants, and vehicles that may be used in the pharmaceutical compositions of this invention include, but are not limited to, ion exchangers, alumina, aluminum stearate, lecithin, self-emulsifying drug delivery systems (SEDDS) such as d-alpha-tocopherol polyethyleneglycol 1000 succinate, surfactants used in pharmaceutical dosage forms such as Tweens, polyethoxylated castor oil such as CREMOPHOR® surfactant (BASF), or other similar polymeric delivery matrices, serum proteins, such as human serum albumin, buffer substances such as phosphates, glycine, sorbic acid, potassium sorbate, partial glyceride mixtures of saturated vegetable fatty acids, water, salts or electrolytes, such as protamine sulfate, disodium hydrogen phosphate, potassium hydrogen phosphate, sodium chloride, zinc salts, colloidal silica, magnesium trisilicate, polyvinyl pyrrolidone, cellulose-based substances, polyethylene glycol, sodium carboxymethylcellulose, polyacrylates, waxes, polyethylene-polyoxypropylene-block polymers, polyethylene glycol and wool fat. Cyclodextrins such as alpha-, beta-, and gamma-cyclodextrin, or chemically modified derivatives such as hydroxyalkylcyclodextrins, including 2- and 3-hydroxypropyl-cyclodextrins, or other solubilized derivatives may also be advantageously used to enhance delivery of compounds of the formulae described herein.
The pharmaceutically active compounds of this invention can be processed in accordance with conventional methods of pharmacy to produce medicinal agents for administration to patients, including humans and other mammals. The pharmaceutical compositions may be subjected to conventional pharmaceutical operations such as sterilization and/or may contain conventional adjuvants, such as preservatives, stabilizers, wetting agents, emulsifiers, buffers, etc. Tablets and pills can additionally be prepared with enteric coatings. Such compositions may also comprise adjuvants, such as wetting, sweetening, flavoring, and perfuming agents.
The amounts of compounds that are administered and the dosage regimen for treating a disease condition with the compounds and/or compositions of this invention depends on a variety of factors, including the age, weight, sex, the medical condition of the subject, the type of disease, the severity of the disease, the route and frequency of administration, and the particular compound employed. Thus, the dosage regimen may vary widely, but can be determined routinely using standard methods. A daily dose of about 0.001 to 100 mg/kg body weight, preferably between about 0.0025 and about 50 mg/kg body weight and most preferably between about 0.005 to 10 mg/kg body weight, may be appropriate. The daily dose can be administered in one to four doses per day. Other dosing schedules include one dose per week and one dose per two day cycle.
For therapeutic purposes, the active compounds of this invention are ordinarily combined with one or more adjuvants appropriate to the indicated route of administration. If administered orally, the compounds may be admixed with lactose, sucrose, starch powder, cellulose esters of alkanoic acids, cellulose alkyl esters, talc, stearic acid, magnesium stearate, magnesium oxide, sodium and calcium salts of phosphoric and sulfuric acids, gelatin, acacia gum, sodium alginate, polyvinylpyrrolidone, and/or polyvinyl alcohol, and then tableted or encapsulated for convenient administration. Such capsules or tablets may contain a controlled-release formulation as may be provided in a dispersion of active compound in hydroxypropylmethyl cellulose.
Pharmaceutical compositions of this invention comprise at least one compound of Formula (I) and/or at least one pharmaceutically acceptable salt thereof, and optionally an additional agent selected from any pharmaceutically acceptable carrier, adjuvant, and vehicle. Alternate compositions of this invention comprise a compound of the Formula (I) described herein, or a prodrug thereof, and a pharmaceutically acceptable carrier, adjuvant, or vehicle.
UTILITY
The compounds of the invention modulate kinase activity, including the modulation of Btk. Other types of kinase activity that may be modulated by the compounds of the instant invention include, but are not limited to, the Tec family of compounds, such as BMX, Btk, ITK, TXK and Tec, and mutants thereof.
Accordingly, compounds of Formula (I) have utility in treating conditions associated with the modulation of kinase activity, and particularly the selective inhibition of Btk activity. Such conditions include B-cell mediated diseases in which cytokine levels are modulated as a consequence of intracellular signaling.
As used herein, the terms “treating” or “treatment” encompass either or both responsive and prophylaxis measures, e.g., measures designed to inhibit or delay the onset of the disease or disorder, achieve a full or partial reduction of the symptoms or disease state, and/or to alleviate, ameliorate, lessen, or cure the disease or disorder and/or its symptoms.
In view of their activity as selective inhibitors of Btk, compounds of Formula (I) are useful in treating cytokine-associated conditions including, but not limited to, inflammatory diseases such as Crohn's and ulcerative colitis, asthma, graft versus host disease, chronic obstructive pulmonary disease; autoimmune diseases such as Graves' disease, rheumatoid arthritis, systemic lupus erythematosis, psoriasis; destructive bone disorders such as bone resorption disease, osteoarthritis, osteoporosis, multiple myeloma-related bone disorder; proliferative disorders such as acute myelogenous leukemia, chronic myelogenous leukemia; angiogenic disorders such as angiogenic disorders including solid tumors, ocular neovasculization, and infantile haemangiomas; infectious diseases such as sepsis, septic shock, and Shigellosis; neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, cerebral ischemias or neurodegenerative disease caused by traumatic injury, oncologic and viral diseases such as metastatic melanoma, Kaposi's sarcoma, multiple myeloma, and HIV infection and CMV retinitis, AIDS, respectively.
More particularly, the specific conditions or diseases that may be treated with the inventive compounds include, without limitation, pancreatitis (acute or chronic), asthma, allergies, adult respiratory distress syndrome, chronic obstructive pulmonary disease, glomerulonephritis, rheumatoid arthritis, systemic lupus erythematosis, scleroderma, chronic thyroiditis, Graves' disease, autoimmune gastritis, diabetes, autoimmune hemolytic anemia, autoimmune neutropenia, thrombocytopenia, atopic dermatitis, chronic active hepatitis, myasthenia gravis, multiple sclerosis, inflammatory bowel disease, ulcerative colitis, Crohn's disease, psoriasis, graft vs. host disease, inflammatory reaction induced by endotoxin, tuberculosis, atherosclerosis, muscle degeneration, cachexia, psoriatic arthritis, Reiter's syndrome, gout, traumatic arthritis, rubella arthritis, acute synovitis, pancreatic β-cell disease; diseases characterized by massive neutrophil infiltration; rheumatoid spondylitis, gouty arthritis and other arthritic conditions, Kawasaki disease, chronic inflammatory demyelinating polyneuropathy (CIDP), dermatomyositis, uveitis, anti-factor-VIII disease, ankylosing spondylitis, myasthenia gravis, Goodpasture's disease, antiphospholipid syndrome, ANCA-associated vasculitis, dermatomyositis/polymyositis, cerebral malaria, chronic pulmonary inflammatory disease, silicosis, pulmonary sarcoidosis, bone resorption disease, allograft rejections, fever and myalgias due to infection, cachexia secondary to infection, myeloid formation, scar tissue formation, ulcerative colitis, pyresis, influenza, osteoporosis, osteoarthritis, acute myelogenous leukemia, chronic myelogenous leukemia, metastatic melanoma, Kaposi's sarcoma, multiple myeloma, sepsis, septic shock, and Shigellosis; Alzheimer's disease, Parkinson's disease, cerebral ischemias or neurodegenerative disease caused by traumatic injury; angiogenic disorders including solid tumors, ocular neovasculization, and infantile haemangiomas; viral diseases including acute hepatitis infection (including hepatitis A, hepatitis B and hepatitis C), HIV infection and CMV retinitis, AIDS, ARC or malignancy, and herpes; stroke, myocardial ischemia, ischemia in stroke heart attacks, organ hypoxia, vascular hyperplasia, cardiac and renal reperfusion injury, thrombosis, cardiac hypertrophy, thrombin-induced platelet aggregation, endotoxemia and/or toxic shock syndrome, conditions associated with prostaglandin endoperoxidase syndase-2, and pemphigus vulgaris. Preferred methods of treatment are those wherein the condition is selected from Crohn's and ulcerative colitis, allograft rejection, rheumatoid arthritis, systemic lupus erythematosis, psoriasis, ankylosing spondylitis, psoriatic arthritis, and pemphigus vulgaris. Alternatively preferred methods of treatment are those wherein the condition is selected from ischemia reperfusion injury, including cerebral ischemia reperfusion injury arising from stroke and cardiac ischemia reperfusion injury arising from myocardial infarction. Another preferred method of treatment is one in which the condition is multiple myeloma.
In addition, the Btk inhibitors of the present invention inhibit the expression of inducible pro-inflammatory proteins such as prostaglandin endoperoxide synthase-2 (PGHS-2), also referred to as cyclooxygenase-2 (COX-2). Accordingly, additional Btk-associated conditions include edema, analgesia, fever and pain, such as neuromuscular pain, headache, pain caused by cancer, dental pain and arthritis pain. The inventive compounds also may be used to treat veterinary viral infections, such as lentivirus infections, including, but not limited to equine infectious anemia virus; or retro virus infections, including feline immunodeficiency virus, bovine immunodeficiency virus, and canine immunodeficiency virus.
When the terms “Btk-associated condition” or “Btk-associated disease or disorder” are used herein, each is intended to encompass all of the conditions identified above as if repeated at length, as well as any other condition that is affected by Btk kinase activity.
“Therapeutically effective amount” is intended to include an amount of a compound of the present invention that is effective when administered alone or in combination to inhibit Btk.
One embodiment provides methods for treating such Btk kinase-associated conditions, comprising administering to a subject in need thereof at least one compound of Formula (I) or a pharmaceutically acceptable salt thereof. A therapeutically-effective amount for treating such conditions may be administered. The methods of the present embodiment may be employed to treat Btk kinase-associated conditions such as treatment of allergic disorders and/or autoimmune and/or inflammatory diseases including, but not limited to, SLE, rheumatoid arthritis, multiple vasculitides, idiopathic thrombocytopenic purpura (ITP), myasthenia gravis, allergic rhinitis, multiple sclerosis (MS), transplant rejection, Type I diabetes, membranous nephritis, inflammatory bowel disease, autoimmune hemolytic anemia, autoimmune thyroiditis, cold and warm agglutinin diseases, Evans syndrome, hemolytic uremic syndrome/thrombotic thrombocytopenic purpura (HUS/TTP), sarcoidosis, Sjögren's syndrome, peripheral neuropathies (e.g., Guillain-Barre syndrome), pemphigus vulgaris, and asthma.
The methods of treating Btk kinase-associated conditions may comprise administering at least one compound of Formula (I) alone or in combination with each other and/or other suitable therapeutic agents useful in treating such conditions. Therapeutically-effective amounts of at least one compound of Formula (I) and other suitable therapeutic agents for treating such conditions may be administered. Accordingly, “therapeutically effective amount” is also intended to include an amount of the combination of compounds claimed that is effective to inhibit Btk. The combination of compounds is preferably a synergistic combination. Synergy, as described, for example, by Chou et al., Adv. Enzyme Regul., 22:27-55 (1984), occurs when the effect (in this case, inhibition of Btk) of the compounds when administered in combination is greater than the additive effect of the compounds when administered alone as a single agent. In general, a synergistic effect is most clearly demonstrated at sub-optimal concentrations of the compounds. Synergy can be in terms of lower cytotoxicity, increased anti-Btk effect, or some other beneficial effect of the combination compared with the individual components.
Exemplary of such other therapeutic agents include corticosteroids, rolipram, calphostin, cytokine-suppressive anti-inflammatory drugs (CSAIDs), 4-substituted imidazo[1,2-A]quinoxalines as disclosed in U.S. Pat. No. 4,200,750; Interleukin-10, glucocorticoids, salicylates, nitric oxide, and other immunosuppressants; nuclear translocation inhibitors, such as deoxyspergualin (DSG); non-steroidal antiinflammatory drugs (NSAIDs) such as ibuprofen, celecoxib and rofecoxib; steroids such as prednisone or dexamethasone; antiviral agents such as abacavir; antiproliferative agents such as methotrexate, leflunomide, FK506 (tacrolimus, PROGRAF®); cytotoxic drugs such as azathiprine and cyclophosphamide; TNF-α inhibitors such as tenidap, anti-TNF antibodies or soluble TNF receptor, and rapamycin (sirolimus or RAPAMUNE®) or derivatives thereof.
The above other therapeutic agents, when employed in combination with the compounds of the present invention, may be used, for example, in those amounts indicated in the Physicians' Desk Reference (PDR) or as otherwise determined by one of ordinary skill in the art. In the methods of the present invention, such other therapeutic agent(s) may be administered prior to, simultaneously with, or following the administration of the inventive compounds. The present invention also provides pharmaceutical compositions capable of treating Btk kinase-associated conditions, including IL-1, IL-6, IL-8, IFNγ and TNF-α-mediated conditions, as described above.
The inventive compositions may contain other therapeutic agents as described above and may be formulated, for example, by employing conventional solid or liquid vehicles or diluents, as well as pharmaceutical additives of a type appropriate to the mode of desired administration (e.g., excipients, binders, preservatives, stabilizers, flavors, etc.) according to techniques such as those well known in the art of pharmaceutical formulation.
Another embodiment provides the compounds of Formula (I) or pharmaceutically acceptable salts thereof, for use in therapy. In the present embodiment, the use in therapy may include the administration of a therapeutically-effective amount of a compound of Formula (I) pharmaceutically acceptable salts thereof.
The present invention also provides the use of the compounds of Formula (I) or pharmaceutically acceptable salts thereof, for the manufacture of a medicament for the treatment or prophylaxis of an allergic disorder and/or autoimmune and/or inflammatory disease. In the present embodiment, the use for the manufacture of a medicament may include the administration of a therapeutically-effective amount of a compound of Formula (I) or pharmaceutically acceptable salts thereof for the treatment of prophylaxis of an allergic disorder and/or autoimmune and/or inflammatory disease.
The present invention also provides the use of the compounds of Formula (I) or pharmaceutically acceptable salts thereof, for the manufacture of a medicament for treatment of cancer. The present embodiment may include the use for the manufacture of a medicament includes the administration of a therapeutically-effective amount of a compound of Formula (I) or pharmaceutically acceptable salts thereof for the treatment of prophylaxis of an allergic disorder and/or autoimmune and/or inflammatory disease.
Accordingly, the present invention further includes compositions comprising one or more compounds of Formula (I) and a pharmaceutically acceptable carrier.
A “pharmaceutically acceptable carrier” refers to media generally accepted in the art for the delivery of biologically active agents to animals, in particular, mammals. Pharmaceutically acceptable carriers are formulated according to a number of factors well within the purview of those of ordinary skill in the art. These include without limitation the type and nature of the active agent being formulated; the subject to which the agent-containing composition is to be administered; the intended route of administration of the composition; and, the therapeutic indication being targeted. Pharmaceutically acceptable carriers include both aqueous and non-aqueous liquid media, as well as a variety of solid and semi-solid dosage forms. Such carriers can include a number of different ingredients and additives in addition to the active agent, such additional ingredients being included in the formulation for a variety of reasons, e.g., stabilization of the active agent, binders, etc., well known to those of ordinary skill in the art. Descriptions of suitable pharmaceutically acceptable carriers, and factors involved in their selection, are found in a variety of readily available sources such as, for example, Remington's Pharmaceutical Sciences, 17th Edition (1985), which is incorporated herein by reference in its entirety.
The compounds of Formula (I) may be administered by any means suitable for the condition to be treated, which may depend on the need for site-specific treatment or quantity of drug to be delivered. Topical administration is generally preferred for skin-related diseases, and systematic treatment preferred for cancerous or pre-cancerous conditions, although other modes of delivery are contemplated. For example, the compounds may be delivered orally, such as in the form of tablets, capsules, granules, powders, or liquid formulations including syrups; topically, such as in the form of solutions, suspensions, gels or ointments; sublingually; bucally; parenterally, such as by subcutaneous, intravenous, intramuscular or intrasternal injection or infusion techniques (e.g., as sterile injectable aqueous or non-aqueous solutions or suspensions); nasally such as by inhalation spray; topically, such as in the form of a cream or ointment; rectally such as in the form of suppositories; or liposomally. Dosage unit formulations containing non-toxic, pharmaceutically acceptable vehicles or diluents may be administered. The compounds may be administered in a form suitable for immediate release or extended release. Immediate release or extended release may be achieved with suitable pharmaceutical compositions or, particularly in the case of extended release, with devices such as subcutaneous implants or osmotic pumps.
Exemplary compositions for topical administration include a topical carrier such as Plastibase (mineral oil gelled with polyethylene).
Exemplary compositions for oral administration include suspensions which may contain, for example, microcrystalline cellulose for imparting bulk, alginic acid or sodium alginate as a suspending agent, methylcellulose as a viscosity enhancer, and sweeteners or flavoring agents such as those known in the art; and immediate release tablets which may contain, for example, microcrystalline cellulose, dicalcium phosphate, starch, magnesium stearate and/or lactose and/or other excipients, binders, extenders, disintegrants, diluents and lubricants such as those known in the art. The inventive compounds may also be orally delivered by sublingual and/or buccal administration, e.g., with molded, compressed, or freeze-dried tablets. Exemplary compositions may include fast-dissolving diluents such as mannitol, lactose, sucrose, and/or cyclodextrins. Also included in such formulations may be high molecular weight excipients such as celluloses (AVICEL®) or polyethylene glycols (PEG); an excipient to aid mucosal adhesion such as hydroxypropyl cellulose (HPC), hydroxypropyl methyl cellulose (HPMC), sodium carboxymethyl cellulose (SCMC), and/or maleic anhydride copolymer (e.g., Gantrez); and agents to control release such as polyacrylic copolymer (e.g., Carbopol 934). Lubricants, glidants, flavors, coloring agents and stabilizers may also be added for ease of fabrication and use.
Exemplary compositions for nasal aerosol or inhalation administration include solutions which may contain, for example, benzyl alcohol or other suitable preservatives, absorption promoters to enhance absorption and/or bioavailability, and/or other solubilizing or dispersing agents such as those known in the art.
Exemplary compositions for parenteral administration include injectable solutions or suspensions which may contain, for example, suitable non-toxic, parenterally acceptable diluents or solvents, such as mannitol, 1,3-butanediol, water, Ringer's solution, an isotonic sodium chloride solution, or other suitable dispersing or wetting and suspending agents, including synthetic mono- or diglycerides, and fatty acids, including oleic acid.
Exemplary compositions for rectal administration include suppositories which may contain, for example, suitable non-irritating excipients, such as cocoa butter, synthetic glyceride esters or polyethylene glycols, which are solid at ordinary temperatures but liquefy and/or dissolve in the rectal cavity to release the drug.
The therapeutically-effective amount of a compound of the present invention may be determined by one of ordinary skill in the art, and includes exemplary dosage amounts for a mammal of from about 0.05 to 1000 mg/kg; 1-1000 mg/kg; 1-50 mg/kg; 5-250 mg/kg; 250-1000 mg/kg of body weight of active compound per day, which may be administered in a single dose or in the form of individual divided doses, such as from 1 to 4 times per day. It will be understood that the specific dose level and frequency of dosage for any particular subject may be varied and will depend upon a variety of factors, including the activity of the specific compound employed, the metabolic stability and length of action of that compound, the species, age, body weight, general health, sex and diet of the subject, the mode and time of administration, rate of excretion, drug combination, and severity of the particular condition. Preferred subjects for treatment include animals, most preferably mammalian species such as humans, and domestic animals such as dogs, cats, horses, and the like. Thus, when the term “patient” is used herein, this term is intended to include all subjects, most preferably mammalian species, that are affected by mediation of Btk enzyme levels.
Examples of compounds of Formula (I) as specified in the “Examples” section below, have been tested in one or more of the assays described below.
Preferred compounds of Formula (I) inhibit Btk enzymes with IC50 values of 6 nM or less, for example, from 0.001 to 6 nM, as measured by the Human Recombinant Btk enzyme assay. More preferably, the compounds of Formula (I) inhibit Btk enzymes with IC50 values of 2 nM and less, for example, from 0.001 to 2 nM. Other preferred compounds inhibit Btk enzymes with IC50 values of 1.0 nM and less, for example, from 0.001 to 1.0 nM.
Preferred compounds of Formula (I) have reduced inhibition of the Jak2 kinase with IC50 values above 50 nM, for example, greater than 250 nM, as measured by the Jak2 tyrosine kinase assay. More preferably, the compounds of Formula (I) inhibit Jak2 enzymes with IC50 values of greater than 400 nM, for example, with IC50 values of greater than 700 nM.
Preferred compounds of Formula (I) have ratios of Jak2 IC50 inhibition values, as measured by the Jak2 tyrosine kinase assay, to Btk IC50 inhibition values, as measured by the Human Recombinant Btk enzyme assay, of 150 and greater, for example, ratios of 300 and greater. More preferably, the compounds of Formula (I) have ratios of Jak2 IC50 inhibition values to Btk IC50 inhibition values of 400 and greater, for example, ratios of 500 and greater.
Preferred compounds of Formula (I) have improved potency in the whole blood BCR-stimulated CD69 expression assay with IC50 values of 250 nM or less, for example, from 0.1 to 250 nM. More preferably, the compounds of Formula (I) have potency in the whole blood BCR-stimulated CD69 expression assay with IC50 values of 160 nM or less, for example, from 0.1 to 160 nM; and with IC50 values of 100 nM or less, for example, from 0.1 to 100 nM.
Methods of Preparation
Compounds of Formula (I) can be prepared using methods shown in Scheme 1. Substituted carbazolecarboxamides 1 (wherein Y is an appropriate group such as Br, Cl or trifluoromethanesulfonyloxy; preferably Br) can be converted to the corresponding boronate esters 2 (R′=alkyl, or both R′ groups taken together form a ring such as 4,4,5,5-tetramethyl-1,3,2-dioxaborolane or 5,5-dimethyl-1,3,2-dioxaborinane) using methods well known in the chemical literature (see, for example, Ishiyama, T. et al., Tetrahedron, 57:9813 (2001), and references cited therein). Examples of such methods are the reaction of 1 with 4,4,4′,4′,5,5,5′,5′-octamethyl-2,2′-bi(1,3,2-dioxaborolane) or 5,5,5′,5′-tetramethyl-2,2′-bi(1,3,2-dioxaborinane) in the presence of a base such as potassium acetate and a suitable catalyst such as 1,1′-bis(diphenylphosphino)ferrocene palladium(II) chloride in a suitable solvent.
Compounds 2 can be converted into compounds 4 of Formula (I), wherein Z represents a substituted monocyclic or fused bicyclic heterocyclic ring (substituent Q in compounds of Formula (I)) by reaction with an appropriate compound 3 (wherein Y′ is an appropriate group such as Br or trifluoromethanesulfonyloxy, preferably Br). This conversion may be achieved by using a suitable base such as potassium carbonate, cesium carbonate or tripotassium phosphate, and a suitable catalyst such as tetrakis (triphenylphosphine)palladium, 1,1′-bis(diphenylphosphino)ferrocene palladium(II) chloride, or 1,1′-bis(di-tert-butylphosphino)ferrocene palladium(II) chloride, in a suitable solvent such as dioxane or tetrahydrofuran, optionally with a suitable cosolvent such as water. Such coupling reactions are commonly known as Suzuki-Miyaura coupling reactions, and are well known in the chemical literature (see, for example, Heravi, M. M., Tetrahedron, 68:9145 (2012), and references cited therein).




Due to the non-symmetric nature of the aromatic rings connected by the single bonds labeled a and b in Scheme 1, and due to limited rotation about these bonds caused by steric hindrance, compounds of Formula (I) display chirality, known as atropisomerism. Thus, under certain conditions, such as chromatography on a chiral stationary phase, the four diastereomers due to the two chiral bonds can be observed as four separate peaks in the chromatogram. Hindered rotation about bond b occurs at a rate which is slow enough to allow isolation of separated atropisomers as different compounds which are stable at normal room temperature. However, hindered rotation about bond a can occur at a rate such that, if separated, these atropisomers will quickly interconvert at room temperature, and the separated atropisomers about bond a may not be capable of storage as separate stable compounds. Thus, compounds of Formula (I) can be isolated either as mixtures of four diastereomers, or as stable pairs of diastereomers, wherein one pair has the (R) configuration about bond b but is a mixture of the (R) and (S) configurations about bond a, and the other pair has the (S) configuration about bond b but is a mixture of the (R) and (S) configurations about bond a.
Compounds 4 of Formula (I) can be separated into pairs of diastereomers 4a, with a single absolute configuration about bond b (either that shown, or the opposite absolute configuration) but a mixture of two interconverting absolute configurations about bond a. Such separation can be achieved using methods known in the art, such as preparative chromatography on a chiral stationary phase. Alternatively, compounds 4a of Formula (I), which are configurationally stable pairs of diastereomers as described above, may be prepared from compounds 2 via the same Suzuki-Miyaura coupling reaction described above but using a single enantiomer 3a (of the absolute configuration shown, or of the opposite absolute configuration), as long as the conditions of the Suzuki-Miyaura coupling are such that racemization of 3a or 4a does not occur.
Compounds 4 of Formula (I) can also be prepared using methods shown in Scheme 2. Substituted boronic esters 5, wherein Z represents a substituted monocyclic or fused bicyclic heterocyclic ring (substituent Q in compounds of Formula (I)), can be reacted with substituted carbazolecarboxamides 1 (wherein Y is an appropriate group such as Br, Cl or trifluoromethanesulfonyloxy, preferably Br) under conditions of the Suzuki-Miyaura coupling reaction as described above, to provide compounds 4 of Formula (I) as mixtures of four diastereomers. Optionally, as described above, such compounds can be separated into pairs of interconverting diastereomers 4a with a single absolute configuration about bond b (either that shown, or the opposite absolute configuration) and a mixture of absolute configurations about bond a. Alternatively, compounds 5 can be separated into single enantiomers 5a, and reacted with compounds 1 in Suzuki-Miyaura coupling reactions, under conditions which will not cause racemization of 5a, to give compounds 4a of Formula (I).




An alternative method for the synthesis of certain compounds 7 of Formula (I) is shown in Scheme 3. A suitably substituted 4-arylimino-1H-benzo[d][1,3]oxazin-2(4H)-one 6 can react with boronic esters 2 under conditions of the Suzuki-Miyaura coupling reaction as described above, to provide compounds 7 of Formula (I) as mixtures of four diastereomers. During the course of the reaction, the 4-arylimino-1H-benzo[d][1,3]oxazin-2(4H)-one moiety present in 6 rearranges to the 3-arylquinazoline-2,4(1H,3H)-dione moiety present in the reaction product 7.




An alternative method for the synthesis of certain compounds 9 of Formula (I) is shown in Scheme 4. A compound 8 of Formula (I), either as a mixture of four diastereomers or a mixture of two interconverting diastereomers, can be treated with an alkylating agent such as iodomethane or trideuteroiodomethane, in the presence of a suitable base such as cesium carbonate or potassium carbonate, in a suitable solvent such as DMF or THF, to give a compound 9 of Formula (I), wherein Ra is the alkyl group derived from the alkylating agent used.




Intermediate compounds 1, used in the preparation of compounds of Formula (I), can be prepared using methods known in the chemical literature, for example in U.S. Pat. No. 8,084,620, and shown in Scheme 5. An appropriately substituted cyclohexanone 10 and a 2-hydrazinylbenzoic acid 11 or a suitable salt of 11 such as a hydrochloric acid salt (wherein Y is a suitable group such as Br, Cl or trifluoromethanesulfonyloxy, preferably Br), both readily prepared by methods well known in the chemical literature, can react in a suitable solvent with an appropriate catalyst, for example ethanol with hydrochloric acid, toluene with p-toluenesulfonic acid or trifluoroacetic acid, or acetic acid (in which case the solvent also can serve as the catalyst), at a suitable temperature to provide the corresponding tetrahydrocarbazole derivative 12. This reaction is commonly known as the Fischer indole synthesis, and is well known in the chemical literature (for example, see Kamata, J. et al., Chem. Pharm. Bull., 52:1071 (2004)). Alternatively, the Fischer indole synthesis can be carried out in two consecutive steps: 10 can react with 11 under suitable conditions (such as in an appropriate solvent such as ethanol or toluene, optionally with a suitable catalyst such as p-toluenesulfonic acid) to form an intermediate hydrazone, which can be isolated and then reacted further under suitable conditions (for example, ethanol with hydrochloric acid, acetic acid with zinc chloride, or toluene with trifluoroacetic acid) to form 12.




The carboxylic acid 12 can be converted to the carboxamide 13 using methods well known in the chemical literature, for example by conversion of 12 to the acid chloride by treatment with oxalyl chloride or thionyl chloride, followed by treatment with ammonia; or by treatment of 12 with ammonia in the presence of a coupling reagent such as carbodiimide or N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride and 1-hydroxybenzotriazole or 1-hydroxy-7-azabenzotriazole. Conversion of the tetrahydrocarbazole 13 to the carbazole 14 can be performed using methods well known in the chemical literature, for example by treatment of 13 with an oxidizing agent such as 2,3-dichloro-5,6-dicyanobenzoquinone in a suitable solvent.
Conversion of the ethoxycarbonyl group of 14 to the substituent R1 of compound 1 can be performed using methods well known in the chemical literature. For example, treatment of 14 with a reagent such as methyllithium or methylmagnesium chloride can provide compound 1 wherein R1 is a 2-hydroxy-2-methylethyl group. Alternatively, treatment of 14 with a reducing agent such as lithium aluminum hydride can provide compound 1 wherein R1 is hydroxymethyl. These and other R1 groups can also be further manipulated to provide still other R1 groups. For example, a compound 1 wherein R1 is hydroxymethyl can be converted to a compound 1 wherein R1 is methoxymethyl by treatment with thionyl chloride followed by treatment of the intermediate compound 1 wherein R1 is chloromethyl with methanol. Additional examples are known in the chemical literature, for example as reported in U.S. Pat. No. 8,084,620.
Intermediate compounds 3 and 5, used in the preparation of compounds 4 of Formula (I), can be prepared by a variety of methods. Some of these methods are shown in Scheme 6. An isatoic anhydride 15 can react with a substituted aniline 16 to produce an amide 17. Such reactions can be carried out under a variety of conditions, for example by heating in a suitable solvent, or by heating in the presence of an auxiliary reagent such as trimethylaluminum. A compound 17 can be converted into a substituted quinazolinedione 18 (which is an example of a compound 3 of Scheme 1), for example by treatment in a suitable solvent with phosgene or triphosgene. Optionally, a compound 18 can be converted to the corresponding boronate ester 19 (which is an example of a compound 5 of Scheme 2) using methods previously described for the conversion of 1 into 2 (see the discussion of Scheme 1 above). Alternatively, a compound 18 can optionally be converted into a compound 20 where Ra is an alkyl group (an example of a compound 3 of Scheme 1) using methods known well known in the chemical literature, for example by treatment with an alkylating agent such as iodomethane in the presence of a suitable base such as cesium carbonate.




A compound 20 can be optionally converted into a corresponding boronate ester 21 (which is an example of a compound 5 of Scheme 2) using the same methods described above. A compound 19 can also be optionally converted into the corresponding compound 21 by methods similar to those described for the conversion of 18 into 20.
As discussed above, the quinazolinedione intermediates 18, 19, 20, and 21 display chirality due to hindered rotation about the bond labeled b. If desired, these intermediates can be resolved into separate enantiomeric atropisomers, for example by chromatography on a chiral stationary phase. The separated enantiomers can then optionally be subjected to the conversions described above (18 to 19, 18 to 20, 19 to 21, or 20 to 21) to provide certain examples of the compounds 3a of Scheme 1 or 5a of Scheme 2.
Intermediate compounds 6 of Scheme 3 can be prepared using the method shown in Scheme 7. An N-substituted isatoic anhydride 22, wherein Ra is an alkyl group, can react with a substituted aniline 16 to produce an amide 23. Such reactions can be carried out under a variety of conditions, for example by heating in a suitable solvent, or by heating in the presence of an auxiliary reagent such as trimethylaluminum. A compound 23 can be converted into a substituted aryliminobenzoxazinone 6, for example by treatment in a suitable solvent with phosgene or triphosgene.




Some additional methods which can be used to prepare compounds 3 and 5, used in the preparation of compounds 4 of the current invention, are shown in Scheme 8. A substituted pyridyl-2-acetic acid 24 or a salt of a substituted pyridyl-2-acetic acid such as a sodium salt (which are either commercially available or can be prepared by methods well known in the literature) can be reacted with an aniline 16 under a variety of methods well known in the chemical literature to provide an amide 25. For example, the reaction can be performed in the presence of a coupling reagent such as O-(7-azabenzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HATU), or a mixture of 1-[3-(dimethylamino)propyl]-3-ethyl-carbodiimide hydrochloride (EDC) and 1-hydroxybenzotriazole hydrate (HOBT). An amide 25 can be converted into the corresponding substituted 1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione 26 (which is an example of an intermediate compound 3 of Scheme 1) by heating with a reagent such as carbonyldiimidazole in an appropriate solvent such as toluene.




Intermediate 26 can optionally be converted into the corresponding boronate ester 27 (which is an example of an intermediate compound 5 of Scheme 2) using methods previously described for the conversion of 1 into 2 (see the discussion of Scheme 1 above). Alternatively, compound 25 can be converted into the corresponding boronate ester 28 using methods previously described, followed by conversion of 28 to 27 by heating with a reagent such as carbonyldiimidazole.
In Scheme 8, the pyridyl ring in the structures shown can also be replaced with another nitrogen heterocycle, such as a thiazole. In this case, the corresponding compounds of structures 26 and 28 will contain a 5H-thiazolo[3,2-c]pyrimidine-5,7(6H)-dione moiety in place of the 1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione moiety shown.
As discussed above, the 1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione compounds 26 and 27 display chirality due to hindered rotation about the bond labeled b. If desired, these intermediates can be resolved into separate enantiomers, for example by chromatography on a chiral stationary phase, to provide certain examples of the intermediate compounds 3a of Scheme 1 or 5a of Scheme 2.




Scheme 9 illustrates another method for preparing certain intermediate compounds 5 of Scheme 2. The amide 25, prepared as shown in Scheme 8, can be treated with a fluorinating agent such as 1-(chloromethyl)-4-fluoro-1,4-diazoniabicyclo[2.2.2]octane bis(tetrafluoroborate) [SELECTFLUOR®] in a suitable solvent to prepare the fluoro-substituted amide 29. This compound can then be converted into the fluorinated boronate ester 30, and subsequently into the intermediate 31 (which is an example of an intermediate compound 5 of Scheme 2) using methods described in Scheme 8.




Preparation of a compound 3 of Scheme 1, wherein Z represents a substituted pyrimidine-1,3-dione moiety, can be achieved using the method shown in Scheme 10, following a general procedure reported by Cao, J. et al. (Synthetic Commun., 39:205 (2009)). Compound 32 can be prepared by combining p-methoxybenzylamine, methyl acrylate and phenyl hypobromoselenoite. This material can be reacted with an appropriate aryl isocyanate 33 (which can be prepared using methods well known in the chemical literature) from the aniline 16 (see Scheme 6) to provide the substituted dihydropyrimidine-1,3-dione 34. Treatment of this compound with an oxidizing agent such as hydrogen peroxide can provide the substituted pyrimidine-1,3-dione 34. Removal of the p-methoxybenzyl group can be achieved using a number of methods reported in the chemical literature, for example by treatment with a mixture of trifluoromethanesulfonic acid and trifluoroacetic acid (as reported by Wu, F. et al., J. Org. Chem., 69:9307 (2004)). The resulting pyrimidine-1,3-dione 35 can be reacted with an aryl boronic acid using conditions described by Jacobsen, M. F. et al. (J. Org. Chem., 71:9183 (2006)) to provide 36, which is an example of an intermediate compound 3 of Scheme 1.
EXAMPLES
Preparation of compounds of the current invention, and intermediates used in the preparation of compounds of the current invention, can be prepared using procedures shown in the following examples and related procedures. The methods and conditions used in these examples, and the actual compounds prepared in these examples, are not meant to be limiting, but are meant to demonstrate how the compounds of the current invention can be prepared. Starting materials and reagents used in these examples, when not prepared by a procedure described herein, are generally either commercially available, or are reported in the chemical literature, or may be prepared by using procedures described in the chemical literature. The invention is further defined in the following Examples. It should be understood that the Examples are given by way of illustration only. From the above discussion and the Examples, one skilled in the art can ascertain the essential characteristics of the invention, and without departing from the spirit and scope thereof, can make various changes and modifications to adapt the invention to various uses and conditions. As a result, the invention is not limited by the illustrative examples set forth herein below, but rather defined by the claims appended hereto.
In the examples given, the phrase “dried and concentrated” generally refers to drying of a solution in an organic solvent over either sodium sulfate or magnesium sulfate, followed by filtration and removal of the solvent from the filtrate (generally under reduced pressure and at a temperature suitable to the stability of the material being prepared).
Column chromatography was generally performed using the flash chromatography technique (Still, W. C. et al., J. Org. Chem., 43:2923 (1978)), or with pre-packed silica gel cartridges using an Isco medium pressure chromatography apparatus (Teledyne Corporation), eluting with the solvent or solvent mixture indicated. Preparative high performance liquid chromatography (HPLC) was performed using a reverse phase column (Waters SunFire C18, Waters XBridge C18, PHENOMENEX® Axia C18, YMC S5 ODS or the like) of a size appropriate to the quantity of material being separated, generally eluting with a gradient of increasing concentration of methanol or acetonitrile in water, also containing 0.05% or 0.1% trifluoroacetic acid or 10 mM ammonium acetate, at a rate of elution suitable to the column size and separation to be achieved. Chiral super-critical fluid chromatographic separation of enantiomers or pairs of diastereomers was performed using conditions described for the individual cases. Mass spectral data were obtained by liquid chromatography mass spectroscopy using electrospray ionization.
Single crystal x-ray diffraction data were collected on a Bruker-AXS APEX2 CCD system using Cu Kα radiation (λ=1.5418 Å). Indexing and processing of the measured intensity data were carried out with the APEX2 software package/program suite (see the APEX2 User Manual, v1.27; Bruker AXS, Inc., WI 53711 USA). When indicated, crystals were cooled in the cold stream of an Oxford Cryosystems cryostream cooler (Cosier, J. et al., J. Appl. Cryst., 19:105 (1986)) during data collection. The structures were solved by direct methods and refined on the basis of observed reflections using the crystallographic package SHELXTL (see the APEX2 User Manual, v1.27; Bruker AXS, Inc., WI 53711 USA). The derived atomic parameters (coordinates and temperature factors) were refined through full matrix least-squares. The function minimized in the refinements was Σw(|Fo|−|Fc|)2. R is defined as Σ∥Fo|-|Fc∥/Σ|Fo| while Rw=[Σw(|Fo|−|Fc|)2/Σw|Fo|2]1/2 where w is an appropriate weighting function based on errors in the observed intensities. Difference maps were examined at all stages of refinement. Hydrogens were introduced in idealized positions with isotropic temperature factors, but no hydrogen parameters were varied. Unit cell parameters were obtained according to the procedure described in Stout et al., X-Ray Structure Determination: A Practical Guide, MacMillan (1968).
Chemical names were determined using CHEMDRAW® Ultra, version 9.0.5 (CambridgeSoft). The following abbreviations are used:
ABBREVIATIONS


    CDI carbonyldiimidazole
    DCM dichloromethane
    DIEA diisopropylethylamine
    DMF N,N-dimethylformamide
    DMSO dimethyl sulfoxide
    dppf 1,1′-bis(diphenylphosphino)ferrocene
    DSC differential scanning calorimetry
    DTT dithiothreitol
    EDC 1-[3-(dimethylamino)propyl]-3-ethyl-carbodiimide hydrochloride
    EDTA ethylenediamine tetraacetate
    EtOAc ethyl acetate
    EtOH ethanol
    g gram(s)
    h hour(s)
    HATU O-(7-azabenzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate
    HOBT 1-hydroxybenzotriazole hydrate
    HPLC High Pressure Liquid Chromatography
    IPA isopropanol
    Me methyl
    MeCN acetonitrile
    MeOH methanol
    min minute(s)
    mmol millimole(s)
    t-butyl tertiary butyl
    TFA trifluoroacetic acid
    THF tetrahydrofuran


Intermediate 1
3-(3-Bromo-2-methylphenyl)-8-fluoroquinazoline-2,4(1H,3H)-dione




Intermediate 1A: 2-Amino-N-(3-bromo-2-methylphenyl)-3-fluorobenzamide




A solution of 8-fluoro-1H-benzo[d][1,3]oxazine-2,4-dione (2.00 g, 11.0 mmol) and 3-bromo-2-methylaniline (4.11 g, 22.1 mmol) in dioxane (20 mL) in sealed reaction vials was heated at 110° C. for 4 days. The cooled mixture was treated with 10% aqueous K2CO3 and stirred at room temperature for 30 min. The mixture was extracted 3 times with DCM, and the combined organic phases were washed with water, dried and concentrated. The residue was triturated with ether to give a gray solid (2.50 g). The mother liquor was concentrated and the residue was again triturated with ether to give a gray solid (230 mg). The two solids were combined to provide 2-amino-N-(3-bromo-2-methylphenyl)-3-fluorobenzamide as a gray solid (2.73 g, 78% yield). Mass spectrum m/z 323, 325 (M+H)+.
Alternative Method:
A suspension of 8-fluoro-1H-benzo[d][1,3]oxazine-2,4-dione (3.00 g, 16.6 mmol) in xylenes (50 mL) was treated with 3-bromo-2-methylaniline (3.08 g, 16.6 mmol) and heated to reflux. After 6 hours, the mixture was allowed to cool to room temperature overnight. The resulting suspension was diluted with hexanes and the precipitate was collected by filtration, rinsed with hexanes and air-dried to provide 2-amino-N-(3-bromo-2-methylphenyl)-3-fluorobenzamide as a white solid (4.50 g, 84% yield). Mass spectrum m/z 323, 325 (M+H)+. 1H NMR (400 MHz, chloroform-d) δ 7.69 (d, J=7.9 Hz, 1H), 7.65 (br. s., 1H), 7.50-7.46 (m, 1H), 7.32 (d, J=8.1 Hz, 1H), 7.19-7.11 (m, 2H), 6.73-6.64 (m, 1H), 5.69 (br. s., 2H), 2.44 (s, 3H).
Intermediate 1:
A solution of 2-amino-N-(3-bromo-2-methylphenyl)-3-fluorobenzamide (5.70 g, 17.6 mmol) in THF (100 mL) was treated with bis(trichloromethyl)carbonate (triphosgene) (6.28 g, 21.2 mmol) at room temperature and stirred for 15 min. The mixture was diluted with EtOAc, carefully treated with saturated aqueous NaHCO3 and stirred at room temperature until gas evolved stopped. The separated organic phase was washed sequentially with saturated aqueous NaHCO3, water and brine, and was dried and concentrated. The residue was triturated with ether to provide 3-(3-bromo-2-methylphenyl)-8-fluoroquinazoline-2,4(1H,3H)-dione as an off-white solid (6 g, 97% yield). Mass spectrum m/z 349, 351 (M+H)+.
Intermediate 2
8-Fluoro-1-methyl-3-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)quinazoline-2,4(1H,3H)-dione




Intermediate 2A: 3-(3-Bromo-2-methylphenyl)-8-fluoro-1-methylquinazoline-2,4(1H,3H)-dione




A solution of 3-(3-bromo-2-methylphenyl)-8-fluoroquinazoline-2,4(1H,3H)-dione (4.80 g, 13.8 mmol) in DMF (25 mL) was treated with Cs2CO3 (13.44 g, 41.2 mmol). The suspension was stirred at room temperature and treated dropwise (but quickly) with iodomethane (4.30 mL, 68.7 mmol) and stirred rapidly at room temperature for 1 h. The mixture was diluted with EtOAc and water (200 mL). The organic phase was separated and washed sequentially with water and brine, then was dried and concentrated to provide 3-(3-bromo-2-methylphenyl)-8-fluoro-1-methylquinazoline-2,4(1H,3H)-dione as a tan glassy solid (4.80 g, 96% yield). Mass spectrum m/z 363, 365 (M+H)+.
Intermediate 2:
A mixture of 3-(3-bromo-2-methylphenyl)-8-fluoro-1-methylquinazoline-2,4(1H,3H)-dione (4.8 g, 13.2 mmol), 4,4,4′,4′,5,5,5′,5′-octamethyl-2,2′-bi(1,3,2-dioxaborolane) (4.36 g, 17.2 mmol), potassium acetate (3.89 g, 39.6 mmol) and PdCl2(dppf) DCM adduct (0.540 g, 0.661 mmol) in dioxane (65 mL) was heated to reflux for 2 h. After cooling to room temperature, the mixture was filtered through CELITE® and the solids were rinsed with EtOAc. The filtrate was diluted with EtOAc, washed with water, and dried and concentrated. The residue was subjected to column chromatography on silica gel (80 g), eluting with EtOAc-hexanes (gradient from 20-50%), to provide 8-fluoro-1-methyl-3-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)quinazoline-2,4(1H,3H)-dione as a white solid (4.61 g, 85% yield). Mass spectrum m/z 411 (M+H)+. 1H NMR (400 MHz, chloroform-d) δ 8.14-8.08 (m, 1H), 7.93 (dd, J=7.5, 1.3 Hz, 1H), 7.48 (ddd, J=14.0, 8.0, 1.5 Hz, 1H), 7.34 (t, J=7.6 Hz, 1H), 7.27-7.20 (m, 2H), 3.88 (d, J=7.9 Hz, 3H), 2.36 (s, 3H), 1.36 (s, 12H).
Intermediates 3 and 3A
8-Fluoro-1-methyl-3-(S)-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)quinazoline-2,4(1H,3H)-dione, and
8-Fluoro-1-methyl-3-(R)-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)quinazoline-2,4(1H,3H)-dione




A sample of racemic 8-fluoro-1-methyl-3-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)quinazoline-2,4(1H,3H)-dione [Intermediate 2] was separated by chiral super-critical fluid chromatography as follows: column: (R,R)-WHELK-O® 1 (5×50 cm, 10 μm); Mobile Phase: CO2-MeOH (76:24) at 290 mL/min, 100 bar, 40° C.; sample preparation: 100 mg/mL in DCM-IPA (6:4); injection: 10.0 mL. The first peak eluting from the column provided the (S) isomer, 8-fluoro-1-methyl-3-(S)-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)quinazoline-2,4(1H,3H)-dione [Intermediate 3] as a white solid. Mass spectrum m/z 411 (M+H)+. 1H NMR (400 MHz, chloroform-d) δ 8.14-8.08 (m, 1H), 7.93 (dd, J=7.5, 1.3 Hz, 1H), 7.48 (ddd, J=14.0, 8.0, 1.5 Hz, 1H), 7.34 (t, J=7.6 Hz, 1H), 7.27-7.20 (m, 2H), 3.88 (d, J=7.9 Hz, 3H), 2.36 (s, 3H), 1.36 (s, 12H).
The second peak eluting from the column provided the (R) isomer, 8-fluoro-1-methyl-3-(R)-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)quinazoline-2,4(1H,3H)-dione [Intermediate 3A] as a white solid. Mass spectrum m/z 411 (M+H)+. 1H NMR (400 MHz, chloroform-d) δ 8.13-8.08 (m, 1H), 7.93 (dd, J=7.5, 1.3 Hz, 1H), 7.48 (ddd, J=13.9, 8.1, 1.5 Hz, 1H), 7.37-7.31 (m, 1H), 7.27-7.20 (m, 2H), 3.88 (d, J=7.9 Hz, 3H), 2.36 (s, 3H), 1.36 (s, 12H).
Intermediate 4
7-(2-Hydroxypropan-2-yl)-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-9H-carbazole-1-carboxamide




A mixture 4-bromo-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide [synthesized according to the procedure described in U.S. Pat. No. 8,084,620, Intermediate 73-2] (3.00 g, 8.64 mmol), 4,4,4′,4′,5,5,5′,5′-octamethyl-2,2′-bi(1,3,2-dioxaborolane) (2.19 g, 8.64 mmol) and potassium acetate (2.12 g, 21.6 mmol) in dioxane (30 mL) was bubbled with nitrogen for 5 min. PdCl2(dppf) DCM adduct (0.353 g, 0.432 mmol) was added and the mixture was bubbled with nitrogen for another 5 min. The reaction vessel was sealed and heated at 90° C. overnight. The cooled mixture was diluted with DCM, washed twice with water, dried and concentrated. The residue was purified by column chromatography on silica gel (40 g+12 g stacked columns), eluting with EtOAc-hexanes, to provide 7-(2-hydroxypropan-2-yl)-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-9H-carbazole-1-carboxamide as a light yellow solid (2.79 g, 82% yield). Mass spectrum m/z 377 (M+H−H2O)+.
Intermediate 5
3-(3-Bromo-2-methylphenyl)-8-fluoro-1-methyl(d3)quinazoline-2,4(1H,3H)-dione




A mixture of 3-(3-bromo-2-methylphenyl)-8-fluoroquinazoline-2,4(1H,3H)-dione [Intermediate 1] (203 mg, 0.581 mmol) and Cs2CO3 (379 mg, 1.16 mmol) in DMF (2.25 mL) was treated with iodomethane-d3 (0.054 mL, 0.872 mmol) and the mixture was stirred at room temperature for 1.5 h. The mixture was diluted with water (ca. 20 mL), and the resulting material was triturated and stirred at room temperature, forming a suspended solid. The precipitate was collected by filtration, washed with water and dried under vacuum to provide 3-(3-bromo-2-methylphenyl)-8-fluoro-1-methyl(d3)quinazoline-2,4(1H,3H)-dione as an off-white solid (189.5 mg, 90% yield). Mass spectrum m/z 366, 368 (M+H)+. 1H NMR (400 MHz, DMSO-d3) δ 7.93 (d, J=7.0 Hz, 1H), 7.79-7.69 (m, 2H), 7.39-7.25 (m, 3H), 2.12 (s, 3H).
Intermediate 6
3-(3-Bromo-2-methylphenyl)-8-methoxy-1-methylquinazoline-2,4(1H,3H)-dione




Intermediate 6A: 2-Amino-N-(3-bromo-2-methylphenyl)-3-methoxybenzamide




A mixture of 3-bromo-2-methylaniline (482 mg, 2.59 mmol) and 8-methoxy-1H-benzo[d][1,3]oxazine-2,4-dione (500 mg, 2.59 mmol) in toluene (20 mL) was treated with 2 M trimethylaluminum in toluene (3.24 mL, 6.47 mmol) at 0° C. The mixture was stirred at room temperature for 10 minutes, then heated at 70° C. overnight. The mixture was cooled to room temperature, treated with 1 M aqueous HCl and extracted 3 times with EtOAc. The combined organic phases were washed sequentially with saturated aqueous NaHCO3 and water, dried and concentrated. The residue was subjected to column chromatography on silica gel (40 g), eluting with EtOAc-hexanes (gradient from 0-100%), to provide 2-amino-N-(3-bromo-2-methylphenyl)-3-methoxybenzamide as a white solid (302 mg, 35% yield). Mass spectrum m/z 335, 337 (M+H)+. 1H NMR (400 MHz, chloroform-d) δ 7.72 (d, J=7.3 Hz, 2H), 7.46 (dd, J=8.0, 0.8 Hz, 1H), 7.17-7.10 (m, 2H), 6.90 (dd, J=7.9, 0.9 Hz, 1H), 6.72-6.66 (m, 1H), 5.88 (br. s., 2H), 3.92 (s, 3H), 2.43 (s, 3H).
Intermediate 6B: 3-(3-Bromo-2-methylphenyl)-8-methoxyquinazoline-2,4(1H,3H)-dione




A solution of 2-amino-N-(3-bromo-2-methylphenyl)-3-methoxybenzamide (302 mg, 0.901 mmol) and triphosgene (321 mg, 1.08 mmol) in THF (20 mL) was stirred at room temperature for 2 h. The mixture was treated carefully with saturated aqueous NaHCO3 and stirred until gas evolution ceased. The mixture was extracted twice with DCM, and the combined organic phases were washed with water, dried and concentrated to provide 3-(3-bromo-2-methylphenyl)-8-methoxyquinazoline-2,4(1H,3H)-dione (339 mg). Mass spectrum m/z 361, 363 (M+H)+.
Intermediate 6: 3-(3-Bromo-2-methylphenyl)-8-methoxy-1-methylquinazoline-2,4(1H,3H)-dione
A mixture of 3-(3-bromo-2-methylphenyl)-8-methoxyquinazoline-2,4(1H,3H)-dione (535 mg, 1.48 mmol), iodomethane (0.185 mL, 2.96 mmol) and Cs2CO3 (965 mg, 2.96 mmol) in THF (20 mL) was stirred at room temperature overnight. The mixture was filtered and concentrated. The residue was dissolved in DCM, washed sequentially with saturated aqueous NaHCO3 and water, dried and concentrated. The residue was subjected to column chromatography on silica gel (40 g), eluting with EtOAc-hexanes (gradient from 0-100%), to provide 3-(3-bromo-2-methylphenyl)-8-methoxy-1-methylquinazoline-2,4(1H,3H)-dione (442 mg). Mass spectrum m/z 375, 377 (M+H)+. 1H NMR (400 MHz, chloroform-d) δ 7.90 (dd, J=7.2, 2.3 Hz, 1H), 7.66 (dd, J=8.0, 1.2 Hz, 1H), 7.31-7.22 (m, 2H), 7.22-7.19 (m, 1H), 7.17-7.13 (m, 1H), 3.97 (s, 3H), 3.89 (s, 3H), 2.23 (s, 3H).
Intermediate 7
3-(3-Bromo-2-methylphenyl)-6-fluoro-1-methylquinazoline-2,4(1H,3H)-dione




Intermediate 7A: 2-Amino-N-(3-bromo-2-methylphenyl)-5-fluorobenzamide




A mixture of 3-bromo-2-methylaniline (1.50 g, 8.06 mmol) and 6-fluoro-1H-benzo[d][1,3]oxazine-2,4-dione (1.46 g, 8.06 mmol) in toluene (40 mL) was cooled on an ice-water bath and treated portionwise with 2 M trimethylaluminum in toluene (10.1 mL, 20.2 mmol). The mixture was stirred at room temperature for 30 min, then was heated at 70° C. overnight. The mixture was cooled to 0° C., carefully treated with 1 M aqueous HCl, and extracted 3 times with EtOAc. The combined organic phases were washed sequentially with saturated aqueous NaHCO3 and water, dried and concentrated. The residue was subjected to column chromatography on silica gel (120 g), eluting with EtOAc-hexanes (gradient from 5-40%), to provide 2-amino-N-(3-bromo-2-methylphenyl)-5-fluorobenzamide (0.893 g, 87% purity, 30% yield). Mass spectrum m/z 323, 325 (M+H)+. 1H NMR (400 MHz, MeOH-d4) δ 7.54 (1H, dd, J=8.03, 0.99 Hz), 7.48 (1H, dd, J=9.68, 3.08 Hz), 7.33 (1H, d, J=7.26 Hz), 7.16 (1H, t, J=7.92 Hz), 7.04-7.12 (1H, m), 6.83 (1H, dd, J=9.02, 4.62 Hz), 2.39 (3H, s).
Intermediate 7B: 3-(3-Bromo-2-methylphenyl)-6-fluoroquinazoline-2,4(1H,3H)-dione




A solution of 2-amino-N-(3-bromo-2-methylphenyl)-5-fluorobenzamide (0.893 g, 2.76 mmol) and triphosgene (0.984 g, 3.32 mmol) in THF (30 mL) was stirred at room temperature for 2 h. The mixture was carefully treated with saturated aqueous NaHCO3 and stirred until gas evolution ceased. The mixture was extracted twice with DCM. The combined organic phases were washed with water, dried and concentrated. The residue was triturated with DCM to give a white solid, isolated by filtration. The filtrate was concentrated and subjected to column chromatography on silica gel (40 g), eluting with EtOAc-hexanes (gradient from 0-80%), to provide additional solid. The two solids were combined to provide 3-(3-bromo-2-methylphenyl)-6-fluoroquinazoline-2,4(1H,3H)-dione as a white solid (845 mg, 87% yield). Mass spectrum m/z 349, 351 (M+H)+.
Intermediate 7:
A mixture of 3-(3-bromo-2-methylphenyl)-6-fluoroquinazoline-2,4(1H,3H)-dione (742 mg, 2.13 mmol), iodomethane (0.159 mL, 2.55 mmol) and Cs2CO3 (1.04 g, 3.19 mmol) in THF (20 mL) was stirred at room temperature overnight. The mixture was filtered and concentrated. The residue was dissolved in DCM and washed sequentially with saturated aqueous NaHCO3 and water, dried and concentrated to provide 3-(3-bromo-2-methylphenyl)-6-fluoro-1-methylquinazoline-2,4(1H,3H)-dione (742 mg, 96% yield). Mass spectrum m/z 363, 365 (M+H)+.
Intermediate 8
(Z)-4-((3-Bromo-2-chlorophenyl)imino)-1-methyl-1H-benzo[d][1,3]oxazin-2(4H)-one




Intermediate 8A: N-(3-Bromo-2-chlorophenyl)-2-(methylamino)benzamide




A mixture of 3-bromo-2-chloroaniline [prepared according to the procedure described in U.S. Pat. No. 8,242,260] (240 mg, 1.16 mmol) and toluene (10 mL) at 0° C. was slowly treated with 2 M trimethylaluminum in toluene (0.99 mL, 1.98 mmol). The mixture was allowed to warm to room temperature and stirred for 15 min. A partial suspension of 1-methyl-1H-benzo[d][1,3]oxazine-2,4-dione (300 mg, 1.52 mmol) in toluene (4 mL) was added slowly. The resulting mixture was heated at 50° C. for 4 h, cooled to 0° C., and treated dropwise with 1 M aqueous HCl until no more gas evolution was observed. The mixture was stirred for 2 h while warming to room temperature, then was extracted with EtOAc. The organic phase was washed sequentially with NaHCO3 and brine, dried and concentrated. The residue was purified by column chromatography on silica gel (80 g), eluting with EtOAc-hexanes (gradient from 0-30%), to provide N-(3-bromo-2-chlorophenyl)-2-(methylamino)benzamide as a yellow solid (110 mg, 28% yield). Mass spectrum m/z 339 (M+H)+. 1H NMR (400 MHz, DMSO-d6) δ 10.00 (s, 1H), 7.78 (dd, J=7.9, 1.5 Hz, 1H), 7.67 (dd, J=8.1, 1.5 Hz, 1H), 7.57 (dd, J=8.0, 1.4 Hz, 1H), 7.53 (d, J=1.3 Hz, 1H), 7.38 (ddd, J=8.4, 7.1, 1.4 Hz, 1H), 7.32 (t, J=8.0 Hz, 1H), 6.70 (d, J=8.4 Hz, 1H), 6.68-6.61 (m, 1H), 2.79 (d, J=5.1 Hz, 3H).
Intermediate 8: (Z)-4-((3-Bromo-2-chlorophenyl)imino)-1-methyl-1H-benzo[d][1,3]oxazin-2(4H)-one
A solution of N-(3-bromo-2-chlorophenyl)-2-(methylamino)benzamide (150 mg, 0.442 mmol) in THF (15 mL) was cooled to 0° C. and treated with triphosgene (197 mg, 0.663 mmol). The mixture was stirred at room temperature for 1 h, then was cooled to 0° C. and treated with water until gas evolution ceased. The mixture was concentrated, and the residue was dissolved in EtOAc and washed sequentially with saturated aqueous NaHCO3, water and brine, and dried and concentrated. The residue was purified by column chromatography on silica gel, eluting with EtOAc-hexanes (gradient from 0-50%), to provide (Z)-4-((3-bromo-2-chlorophenyl)imino)-1-methyl-1H-benzo[d][1,3]oxazin-2(4H)-one as a beige solid (130 mg, 81% yield). 1H NMR (400 MHz, chloroform-d) δ 8.33 (dd, J=7.8, 1.4 Hz, 1H), 7.72-7.61 (m, 1H), 7.41 (dd, J=7.9, 1.5 Hz, 1H), 7.36-7.28 (m, 1H), 7.16-7.08 (m, 2H), 7.07-7.01 (m, 1H), 3.55 (s, 3H).
Intermediate 9
3-(3-Bromo-2-chlorophenyl)-8-fluoro-1-methylquinazoline-2,4(1H,3H)-dione




Intermediate 9A: 2-Amino-N-(3-bromo-2-chlorophenyl)-3-fluorobenzamide




A mixture of 3-bromo-2-chloroaniline [prepared according to the procedure described in U.S. Pat. No. 8,242,260] (600 mg, 2.91 mmol) and toluene (10 mL) was cooled to 0° C. and slowly treated with 2 M trimethylaluminum in toluene (2.47 mL, 4.94 mmol). The mixture was allowed to warm to room temperature and stirred for 15 min. Next, 8-fluoro-1H-benzo[d][1,3]oxazine-2,4-dione (684 mg, 3.78 mmol) was added in one portion and the mixture was heated to 50° C. for 16 h. The mixture was cooled to 0° C. and treated dropwise with 1 M aqueous HCl until gas evolution stopped, and stirred for 2 h while allowing to warm to room temperature. The mixture was extracted 3 times with EtOAc. The combined organic phases were washed sequentially with saturated aqueous NaHCO3 and brine, dried and concentrated. The residue was purified by column chromatography on silica gel (24 g), eluting with EtOAc-hexanes (gradient from 0-30%), to provide 2-amino-N-(3-bromo-2-chlorophenyl)-3-fluorobenzamide as a pale yellow solid (350 mg, 35% yield). Mass spectrum m/z 343 (M+H)+. 1H NMR (400 MHz, chloroform-d) δ 8.46 (dd, J=8.4, 1.3 Hz, 1H), 8.42 (br. s., 1H), 7.43 (dd, J=8.0, 1.4 Hz, 1H), 7.34 (d, J=8.1 Hz, 1H), 7.22 (t, J=8.3 Hz, 1H), 7.15 (ddd, J=11.0, 8.0, 1.2 Hz, 1H), 6.69 (td, J=8.0, 5.1 Hz, 1H), 5.72 (br. s., 2H).
Intermediate 9B: 3-(3-Bromo-2-chlorophenyl)-8-fluoroquinazoline-2,4(1H,3H)-dione




Triphosgene (453 mg, 1.53 mmol) was added in one portion to a solution of amino-N-(3-bromo-2-chlorophenyl)-3-fluorobenzamide (350 mg, 1.02 mmol) in THF (10 mL) at 0° C. The mixture was stirred at room temperature for 1 h, then cooled to 0° C. and treated with water until no gas evolution was observed. The mixture was concentrated and the residue was dissolved in EtOAc, washed sequentially with saturated aqueous NaHCO3, water and brine, and dried and concentrated. The residue was purified by column chromatography on silica gel (24 g), eluting with EtOAc-hexanes (gradient from 0-50%), to provide 3-(3-bromo-2-chlorophenyl)-8-fluoroquinazoline-2,4(1H,3H)-dione as a yellow solid (320 mg, 85% yield). Mass spectrum m/z 369 (M+H)+. 1H NMR (400 MHz, chloroform-d) δ 8.54 (br. s., 1H), 7.97 (d, J=8.1 Hz, 1H), 7.77 (dd, J=6.8, 2.6 Hz, 1H), 7.46 (ddd, J=9.8, 8.3, 1.2 Hz, 1H), 7.36-7.29 (m, 2H), 7.24 (td, J=8.0, 4.8 Hz, 1H).
Intermediate 9:
Iodomethane (0.102 mL, 1.623 mmol) was added slowly to a mixture of 3-(3-bromo-2-chlorophenyl)-8-fluoroquinazoline-2,4(1H,3H)-dione (300 mg, 0.812 mmol), DMF (5 mL) and Cs2CO3 (529 mg, 1.62 mmol). The mixture was stirred at room temperature for 2 h, then was diluted with EtOAc, washed sequentially with water and brine, and dried and concentrated. The residue was purified by column chromatography on silica gel (24 g), eluting with EtOAc-hexanes (gradient from 0-30%), to provide 3-(3-bromo-2-chlorophenyl)-8-fluoro-1-methylquinazoline-2,4(1H,3H)-dione as a yellow solid (280 mg, 90% yield). Mass spectrum m/z 383 (M+H)+. 1H NMR (400 MHz, chloroform-d) δ 8.09 (dq, J=7.8, 0.8 Hz, 1H), 7.79-7.71 (m, 1H), 7.49 (ddd, J=13.9, 8.1, 1.5 Hz, 1H), 7.32-7.29 (m, 2H), 7.29-7.22 (m, 2H), 3.88 (s, 1.5H, 3.86 (s, 1.5H).
Intermediate 10
2-(3-Bromo-2-methylphenyl)-5-methoxy-1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione




Intermediate 10A: Ethyl 2-(3-methoxypyridin-2-yl)acetate




A stirred solution of diisopropylamine (0.385 mL, 2.70 mmol) in THF (2 mL) at 0° C. was treated slowly with 1.6 M n-butyllithium in hexanes (1.69 mL, 2.70 mmol). The mixture was stirred for 15 min, then was added via syringe over 5 min to a stirred solution of 3-methoxy-2-picoline (0.133 g, 1.08 mmol) and diethyl carbonate (0.262 mL, 2.16 mmol) in THF (5 mL) at −78° C. After stirring for 45 min more, the cooling bath was removed and stirring was continued overnight at room temperature. The mixture was treated with saturated aqueous NH4Cl and diluted with EtOAc. The organic phase was separated, washed sequentially with saturated aqueous NaHCO3 and brine, and dried and concentrated. The residue was purified by column chromatography on silica gel (12 g), eluting with 50% EtOAc-hexanes, to provide ethyl 2-(3-methoxypyridin-2-yl)acetate as an oil (0.17 g, 81% yield). Mass spectrum m/z 196 (M+H)+.
Intermediate 10B: Sodium 2-(3-methoxypyridin-2-yl)acetate




A stirred solution of ethyl 2-(3-methoxypyridin-2-yl)acetate (0.17 g, 0.871 mmol) in THF (2.5 mL) at room temperature was treated with 3 M aqueous NaOH (0.581 mL, 1.74 mmol). After 7 h, the mixture was concentrated to remove the THF and the aqueous residue was frozen on dry ice and lyophilized to provide sodium 2-(3-methoxypyridin-2-yl)acetate as a white solid. A quantitative yield was assumed and the material was used without further purification. Mass spectrum m/z 168 (M+H)+.
Intermediate 10C: N-(3-Bromo-2-methylphenyl)-2-(3-methoxypyridin-2-yl)acetamide




A mixture of sodium 2-(3-methoxypyridin-2-yl)acetate (0.166 g, 0.871 mmol), 3-bromo-2-methylaniline (0.118 mL, 0.958 mmol), DIEA (0.608 mL, 3.48 mmol) and HATU (0.397 g, 1.045 mmol) in DMF (4.0 mL) was stirred at room temperature. After 1 h, the mixture was diluted with EtOAc and washed twice with 10% aqueous LiCl, then with brine, dried and concentrated. The residue was purified by column chromatography on silica gel to provide N-(3-bromo-2-methylphenyl)-2-(3-methoxypyridin-2-yl)acetamide as a pale yellow solid (0.213 g, 73% yield). Mass spectrum m/z 335, 337 (M+H)+.
Intermediate 10:
A mixture of N-(3-bromo-2-methylphenyl)-2-(3-methoxypyridin-2-yl)acetamide (0.136 g, 0.406 mmol) and CDI (0.263 g, 1.62 mmol) in toluene (2 mL) was heated at 110° C. After 4 h, the mixture was cooled, diluted with EtOAc and washed sequentially with water and brine. The organic phase was dried and concentrated, and the residue was purified by column chromatography on silica gel (24 g), eluting with 40% EtOAc-hexanes, to provide 2-(3-bromo-2-methylphenyl)-5-methoxy-1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione as a yellow solid (0.0729 g, 50% yield). Mass spectrum m/z 361, 363 (M+H)+.
Intermediate 11
2-(3-Bromo-2-methylphenyl)-6-methoxy-1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione




This intermediate was synthesized from 4-methoxy-2-methylpyridine following the general synthetic route described for Intermediate 10. Mass spectrum m/z 361, 363 (M+H)+.
Intermediate 12
2-(3-Bromo-2-methylphenyl)-7-methoxy-1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione




This intermediate was synthesized from 3-methoxy-6-picoline following the general synthetic route described for Intermediate 10. Mass spectrum m/z 361, 363 (M+H)+.
Intermediate 13
5-Chloro-2-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione(racemic)




Intermediate 13A: Diethyl 2-(3-chloropyridin-2-yl)malonate




A mixture of 3-chloro-2-fluoropyridine (5.00 g, 38.0 mmol), diethyl malonate (14.61 g, 91 mmol) and Cs2CO3 (29.7 g, 91 mmol) in DMSO (42 mL) was heated at 100° C. for 7 h. After stirring overnight at room temperature, the mixture was diluted with EtOAc and washed twice with water, then with brine. The combined aqueous layers were extracted with EtOAc, and the combined organic phases were dried and concentrated to give crude diethyl 2-(3-chloropyridin-2-yl)malonate as a colorless oil, used without further purification. Mass spectrum m/z 272 (M+H)+.
Intermediate 13B: Ethyl 2-(3-chloropyridin-2-yl)acetate




A mixture of diethyl 2-(3-chloropyridin-2-yl)malonate (10.32 g, 38 mmol), sodium chloride (5.55 g, 95 mmol) and water (3.4 mL, 190 mmol) in DMSO (40 mL) was heated at 145° C. for 8 h. The mixture was cooled to room temperature, diluted with EtOAc and washed twice with water, then with brine. The organic phase was dried and concentrated to provide crude ethyl 2-(3-chloropyridin-2-yl)acetate, used without further purification. Mass spectrum m/z 200 (M+H)+.
Intermediate 13C: Sodium 2-(3-chloropyridin-2-yl)acetate




A solution of ethyl 2-(3-chloropyridin-2-yl)acetate (7.59 g, 38 mmol) in THF (76 mL) was treated at room temperature with 3 M aqueous NaOH (25.3 mL, 76 mmol). The mixture was stirred at room temperature overnight and concentrated to remove the THF. The aqueous residue was frozen on dry ice and lyophilized to give sodium 2-(3-chloropyridin-2-yl)acetate as an off-white solid, used without further purification. Mass spectrum m/z 172, (M+H)+.
Intermediate 13D: N-(3-Bromo-2-methylphenyl)-2-(3-chloropyridin-2-yl)acetamide




A mixture of sodium 2-(3-chloropyridin-2-yl)acetate (7.39 g, 38 mmol), 3-bromo-2-methylaniline (4.7 mL, 38.4 mmol), DIEA (13.3 mL, 76 mmol) and HATU (14.59 g, 38.4 mmol) in DMF (127 mL) was stirred at room temperature. After 90 min the mixture was diluted with EtOAc and washed twice with 10% LiCl, then with brine. The combined aqueous layers were extracted with EtOAc, and the combined organic phases were dried and concentrated to a small volume. The solution was seeded with a crystal from an earlier batch and allowed to stand overnight to provide a precipitate which was collected by filtration and washed with 50% EtOAc-hexanes to provide a white solid. The filtrate was concentrated and recrystallized similarly three times to provide additional solid. The solids were combined to give N-(3-bromo-2-methylphenyl)-2-(3-chloropyridin-2-yl)acetamide as a white solid (11.43 g, 89% yield). Mass spectrum m/z 339, 341 (M+H)+. 1H NMR (400 MHz, chloroform-d) δ 9.76 (br. s., 1H), 8.52 (d, j=3.5 Hz, 1H), 7.92 (d, J=7.9 Hz, 1H), 7.80 (dd, J=8.1, 1.1 Hz, 1H), 7.36 (d, J=7.9 Hz, 1H), 7.32-7.23 (m, 1H), 7.06 (t, J=8.0 Hz, 1H), 4.16 (s, 2H), 2.39 (s, 3H).
Intermediate 13E: 2-(3-Chloropyridin-2-yl)-N-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)acetamide




A mixture of N-(3-bromo-2-methylphenyl)-2-(3-chloropyridin-2-yl)acetamide (4.0 g, 11.8 mmol) and 4,4,4′,4′,5,5,5′,5′-octamethyl-2,2′-bi(1,3,2-dioxaborolane) (3.29 g, 13.0 mmol) in DMSO (5 mL) and dioxane (25 mL) was bubbled with argon for 7 min, followed by addition of potassium acetate (2.89 g, 29.4 mmol). Argon bubbling was continued for 7 min after which PdCl2(dppf) DCM adduct (0.481 g, 0.589 mmol) was added. The mixture was heated at 90° C. for 7 h. The cooled mixture was diluted with EtOAc and filtered through CELITE®. The filtrate was washed sequentially with water and brine. The combined aqueous layers were extracted with EtOAc, and the combined organic phases were dried and concentrated. The residue was recrystallized from EtOAc to provide a white solid. The mother liquor was concentrated and the residue was recrystallized from EtOAc. The two solids were combined to provide 2-(3-chloropyridin-2-yl)-N-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)acetamide as a white solid (3.88 g, 85% yield). Mass spectrum m/z 387, 389 (M+H)+.
Intermediate 13:
A mixture of 2-(3-chloropyridin-2-yl)-N-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)acetamide (0.192 g, 0.497 mmol) and CDI (0.322 g, 1.99 mmol) in toluene (2 mL) was heated at 110° C. After 5 h, the cooled mixture was diluted with EtOAc and washed sequentially with water and brine. The combined aqueous layers were extracted with EtOAc, and the combined organic phases were dried and concentrated. The residue was purified by column chromatography on silica gel, eluting with EtOAc-hexanes, to provide racemic 5-chloro-2-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione as a bright yellow solid (0.133 g, 65% yield). Mass spectrum m/z 413 (M+H)+.
Intermediates 14 and 15
5-Chloro-2-(R)-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione (I-14), and
5-Chloro-2-(S)-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione (I-15)




A sample of racemic 5-chloro-2-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione [Intermediate 13] was separated by chiral super-critical fluid chromatography as follows: column: WHELK-O® RR (3×25 cm, 5 μm); Mobile Phase: CO2-MeOH (55:45) at 200 mL/min, 100 bar, 35° C.; sample preparation: 96 mg/mL in MeCN-DCM (1:4); injection: 5 mL. The first peak eluting from the column provided 5-chloro-2-(R)-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione [Intermediate 14]. The second peak eluting from the column provided 5-chloro-2-(S)-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione [Intermediate 15]. The mass spectrum and 1H NMR for both enantiomers were the same as those for Intermediate 13.
The absolute configuration of Intermediate 15 was confirmed by single crystal x-ray analysis of crystals prepared by dissolving the compound in excess acetone and slowly evaporating the solvent at room temperature. Unit cell dimensions: a=19.6161(8) Å, b=9.1411(4) Å, c=12.7541(6) Å, α=90°, β=113.165(2)°, γ=90°; Space group: C2; Molecules of Intermediate 35/asymmetric unit (Z′): 1; Density, calc g-cm−3: 1.304. Fractional atomic coordinates at room temperature are given in Table 1, and a depiction of the structure is given in FIG. 1.







TABLE 1







Fractional Atomic Coordinates for Intermediate 15 at Room Temperature














Atom
X
Y
Z
Atom
X
Y
Z

















C11
−0.1755
−0.1003
0.3365
C21
0.0166
−0.2724
0.3622


O1
0.2261
0.6937
0.2037
O3
−0.0487
0.3988
0.3318


C1
0.2132
0.8050
0.1156
O4
0.1356
0.0809
0.3596


C2
0.1347
0.7655
0.0313
H1
0.1356
0.3551
0.5384


O2
0.1028
0.6968
0.1045
H2
0.2203
0.5422
0.5706


B1
0.1597
0.6467
0.1980
H3
0.2313
0.6587
0.4179


C3
0.1403
0.4028
0.4772
H4
0.0388
0.8624
−0.0807


C4
0.1906
0.5142
0.4966
H5
0.1101
0.9509
−0.0687


C5
0.1012
0.4308
0.2727
H6
0.0796
0.9586
0.0279


C6
0.1966
0.5843
0.4048
H7
0.0613
0.2788
0.1461


C7
0.0966
0.3615
0.3668
H8
0.0655
0.4356
0.0994


C8
0.1517
0.5466
0.2926
H9
0.0019
0.3967
0.1400


C9
0.0863
0.8966
−0.0281
H10
0.1556
0.5689
−0.0231


C10
0.0532
0.3809
0.1539
H11
0.1519
0.6967
−0.1073


C11
0.1298
0.6565
−0.0585
H12
0.0787
0.6339
−0.1029


C12
0.2226
0.9474
0.1724
H13
0.1871
0.9571
0.2066


C13
0.2710
0.7829
0.0643
H14
0.2151
1.0241
0.1175


N1
0.0457
0.2404
0.3528
H15
0.2718
0.9545
0.2304


C14
0.0746
0.1035
0.3564
H16
0.3176
0.8231
0.1147


N2
0.0270
−0.0148
0.3551
H17
0.2550
0.8312
−0.0083


C15
−0.0287
0.2712
0.3397
H18
0.2769
0.6801
0.0543


C16
−0.0453
0.0086
0.3467
H19
−0.1208
0.1615
0.3296


C17
−0.0720
0.1477
0.3375
H20
0.1039
−0.1652
0.3637


C18
0.0561
−0.1540
0.3608
H21
−0.0838
−0.3378
0.3584


C19
−0.0563
−0.2557
0.3562
H22
0.0370
−0.3652
0.3671


C20
−0.0863
−0.1218
0.3472
—
—
—
—









Alternatively, a sample of racemic 5-chloro-2-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione [Intermediate 13] was separated by chiral super-critical fluid chromatography as follows: column: WHELK-O® RR (3×25 cm, 5 μm); Mobile Phase: CO2—CH3CN (55:45) at 200 mL/min, 100 bar, 35° C.; sample preparation: 96 mg/mL in MeCN-DCM (1:4); injection: 5 mL. The first peak eluting from the column provided one atropisomer of 5-chloro-2-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione [Intermediate 15]. The material could be further purified by dissolving in THF/hexanes and collecting the resulting yellow solid.
Intermediate 16
4-Fluoro-2-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione




Intermediate 16A: N-(3-Bromo-2-methylphenyl)-2-(pyridin-2-yl)acetamide




A mixture of 3-bromo-2-methylaniline (2.36 mL, 19.1 mmol), pyridin-2-yl-acetic acid hydrochloride (3.32 g, 19.1 mmol), EDC (5.50 g, 28.7 mmol), HOBT (0.146 g, 0.956 mmol) and DIEA (13.4 mL, 76 mmol) in 1,2-dichloroethane (70 mL) was stirred at room temperature for 3 h. The mixture was diluted with DCM and washed twice with water. The combined aqueous layers were extracted with DCM. The combined organic phases were dried and concentrated to a reduced volume, then diluted with hexane. The precipitated crystals were collected by filtration. The filtrate was concentrated and recrystallized from DCM-hexane to provide additional solid. The two batches of solid were combined to provide N-(3-bromo-2-methylphenyl)-2-(pyridin-2-yl)acetamide as a tan solid (4.78 g. 82% yield). Mass spectrum m/z 305, 307 (M+H)+.
Intermediate 16B: N-(3-Bromo-2-methylphenyl)-2-fluoro-2-(pyridin-2-yl)acetamide




A mixture of N-(3-bromo-2-methylphenyl)-2-(pyridin-2-yl)acetamide (0.5 g, 1.64 mmol) and 1-(chloromethyl)-4-fluoro-1,4-diazoniabicyclo[2.2.2]octanebis-(tetrafluoroborate) [SELECTFLUOR®] (0.871 g, 2.46 mmol) in 1,2-dichloroethane (8.0 mL) was heated at 90° C. overnight. Additional SELECTFLUOR® (300 mg) was added and the mixture was heated overnight at 60° C. A third portion of SELECTFLUOR® (150 mg) was added and heating was continued for another 3 h. The cooled mixture was partitioned between EtOAc and water. The organic phase was washed with brine, dried and concentrated. The residue was purified by column chromatography on silica gel (80 g), eluting with EtOAc-hexanes (35%, then 50%), to provide N-(3-bromo-2-methylphenyl)-2-fluoro-2-(pyridin-2-yl)acetamide as a light brown solid (0.177 g, 33% yield). 1H NMR (400 MHz, chloroform-d) δ 8.84 (br. s., 1H), 8.67 (dt, J=4.8, 0.9 Hz, 1H), 7.91-7.79 (m, 2H), 7.60 (d, J=7.7 Hz, 1H), 7.44-7.35 (m, 2H), 7.08 (t, J=8.1 Hz, 1H), 6.08-5.93 (m, 1H), 2.41 (s, 3H).
Intermediate 16C: 2-Fluoro-N-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-2-(pyridin-2-yl)acetamide




A mixture N-(3-bromo-2-methylphenyl)-2-fluoro-2-(pyridin-2-yl)acetamide (0.122 g, 0.378 mmol), 4,4,4′,4′,5,5,5′,5′-octamethyl-2,2′-bi(1,3,2-dioxaborolane) (0.105 g, 0.415 mmol) and potassium acetate (0.093 g, 0.944 mmol) in DMSO (0.6 mL) and dioxane (3.0 mL) was bubbled with nitrogen for 5 min, followed by the addition of PdCl2(dppf) DCM adduct (0.015 g, 0.019 mmol). After bubbling with nitrogen for another 5 min, the mixture was heated at 90° C. overnight. The cooled mixture was diluted with EtOAc, washed twice with water, dried and concentrated. The residue was subjected to column chromatography on silica gel (40 g), eluting with EtOAc-hexanes (30%, then 50%), to provide 2-fluoro-N-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-2-(pyridin-2-yl)acetamide as a pale-colored oil (0.11 g, 79% yield). Mass spectrum m/z 371 (M+H)+.
Intermediate 16:
A mixture of 2-fluoro-N-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-2-(pyridin-2-yl)acetamide (0.155 g, 0.419 mmol) and CDI (0.272 g, 1.675 mmol) in toluene (3.0 mL) was stirred at 110° C. for 6 h. The cooled mixture was partitioned between EtOAc and water, and the aqueous layer was extracted with EtOAc. The combined organic layers were washed with brine, dried and concentrated, and the residue was purified by column chromatograph on silica gel, eluting with EtOAc-hexanes, to provide 4-fluoro-2-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione as a yellow solid (43.1 mg, 26% yield). Mass spectrum m/z 397 (M+H)+.
Intermediate 17
6-(2-Methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-5H-thiazolo[3,2-c]pyrimidine-5,7(6H)-dione




Intermediate 17A: N-(3-Bromo-2-methylphenyl)-2-(thiazol-2-yl)acetamide




A mixture of 3-bromo-2-methylaniline (0.764 mL, 6.20 mmol), 1,3-thiazol-2-ylacetic acid (0.74 g, 5.17 mmol) and DIEA (1.63 mL, 9.30 mmol) in DMF (15 mL) was treated with HATU (2.36 g, 6.20 mmol). After stirring overnight, the mixture was diluted with EtOAc, washed twice with 10% aqueous LiCl followed by brine, and the combined aqueous layers were extracted with EtOAc. The combined organic layers were dried and concentrated, and the residue was purified by column chromatography on silica gel, eluting with EtOAc-hexanes, to give N-(3-bromo-2-methylphenyl)-2-(thiazol-2-yl)acetamide as a white solid (0.681 g, 42% yield). 1H NMR (400 MHz, chloroform-d) δ 9.84-9.65 (m, 1H), 7.91 (d, J=7.9 Hz, 1H), 7.84 (d, J=3.3 Hz, 1H), 7.42-7.35 (m, 2H), 7.07 (t, J=8.0 Hz, 1H), 4.18 (s, 2H), 2.38 (s, 3H).
Intermediate 17B: N-(2-Methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-2-(thiazol-2-yl)acetamide




A mixture N-(3-bromo-2-methylphenyl)-2-(thiazol-2-yl)acetamide (0.53 g, 1.70 mmol) 4,4,4′,4′,5,5,5′,5′-octamethyl-2,2′-bi(1,3,2-dioxaborolane) (0.476 g, 1.87 mmol) and potassium acetate (0.418 g, 4.26 mmol) in DMSO (1.6 mL) and dioxane (8 mL) was bubbled with nitrogen for 5 min, followed by the addition of PdCl2(dppf) DCM adduct (0.070 g, 0.085 mmol). After bubbling with nitrogen for another 5 min, the mixture was heated at 90° C. for 7 h. The cooled mixture was diluted with EtOAc and filtered through CELITE®. The filtrate was washed sequentially with water and brine, and dried and concentrated. The residue was purified by column chromatography on silica gel (40 g), eluting with 50% EtOAc-hexanes, to give N-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-2-(thiazol-2-yl)acetamide as an off-white solid (0.45 g, 74% yield). Mass spectrum m/z 359 (M+H)+.
Intermediate 17:
A mixture of N-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-2-(thiazol-2-yl)acetamide (0.45 g, 1.26 mmol) and CDI (0.815 g, 5.02 mmol) in toluene (6.5 mL) was heated at 110° C. for 2 h. The cooled mixture was partitioned between EtOAc and water. The organic layer was washed with brine, and the combined aqueous layers were extracted with EtOAc. The combined organic layers were dried and concentrated. The residue was purified by column chromatography on silica gel (40 g), eluting with 70% EtOAc-hexanes, to give 6-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-5H-thiazolo[3,2-c]pyrimidine-5,7(6H)-dione as a tan solid (34% yield). Mass spectrum m/z 385 (M+H)+.
Intermediate 18
5-Fluoro-2-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione(racemic)




Intermediate 18A: Diethyl 2-(3-fluoropyridin-2-yl)malonate




A mixture of 2,3-difluoropyridine (2.00 g, 17.4 mmol), Cs2CO3 (13.59 g, 41.7 mmol) and diethyl malonate (6.68 g, 41.7 mmol) in DMSO (19 mL) was heated at 100° C. for 4.5 h. The mixture was poured onto ice, diluted with EtOAc, and the organic phase was separated, washed sequentially with water and brine, and dried and concentrated. The residue was purified by column chromatography on silica gel (80 g), eluting with EtOAc-hexanes (sequentially 10%, 20% and 30%), to provide diethyl 2-(3-fluoropyridin-2-yl)malonate as a pale colored oil (2.68 g, 60% yield). 1H NMR (400 MHz, chloroform-d) δ 8.42 (dt, J=4.6, 1.3 Hz, 1H), 7.43 (ddd, J=9.4, 8.3, 1.4 Hz, 1H), 7.30 (dt, J=8.5, 4.3 Hz, 1H), 5.09 (d, J=1.1 Hz, 1H), 4.30 (q, J=7.0 Hz, 4H), 1.33-1.26 (m, 6H).
Intermediate 18B: Ethyl 2-(3-fluoropyridin-2-yl)acetate




A mixture of diethyl 2-(3-fluoropyridin-2-yl)malonate (2.68 g, 10.5 mmol), sodium chloride (0.675 g, 11.6 mmol) and water (0.378 mL, 21.0 mmol) in DMSO (15 mL) was heated at 145° C. for 4.5 h. The mixture was cooled, diluted with EtOAc and washed sequentially with water and brine. The organic phase was dried and concentrated to provide ethyl 2-(3-fluoropyridin-2-yl)acetate as a pale colored oil (1.9 g, 99% yield). Mass spectrum m/z 184 (M+H)+.
Intermediate 18C: Sodium 2-(3-fluoropyridin-2-yl)acetate




A stirred solution of ethyl 2-(3-fluoropyridin-2-yl)acetate (1.90 g, 10.4 mmol) in THF (26 mL) was treated with 3 M aqueous NaOH (6.9 mL, 20.7 mmol) and stirred at room temperature overnight. The mixture was concentrated to remove the THF, and the residual aqueous solution was frozen and lyophilized to provide sodium 2-(3-fluoropyridin-2-yl)acetate as a white solid, used without further purification. Mass spectrum m/z 156 (M+H)+.
Intermediate 18D: N-(3-Bromo-2-methylphenyl)-2-(3-fluoropyridin-2-yl)acetamide




A mixture of sodium 2-(3-fluoropyridin-2-yl)acetate (1.85 g, 10.4 mmol), 3-bromo-2-methylaniline (1.4 mL, 11.4 mmol), DIEA (5.4 mL, 31.1 mmol) and HATU (4.73 g, 12.4 mmol) in DMF (30 mL) was stirred at room temperature for 1.25 h. The mixture was diluted with EtOAc and washed twice with 10% aqueous LiCl, then with brine. The combined aqueous layers were extracted with EtOAc, and the combined organic phases were dried and concentrated. The residue was dissolved in hot EtOAc, allowed to cool, and the resulting white solid collected by filtration and washed with 60% EtOAc-hexanes. The combined filtrates were concentrated and the residue was recrystallized twice using the same procedure. The residue from concentration of the final filtrate was purified by column chromatography on silica gel, eluting with EtOAc-hexanes, to provide a solid which was combined with the recrystallized batches to provide N-(3-bromo-2-methylphenyl)-2-(3-fluoropyridin-2-yl)acetamide as a white solid (2.029 g, 61% yield). Mass spectrum m/z 323, 325 (M+H)+.
Intermediate 18E: 2-(3-Fluoropyridin-2-yl)-N-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)acetamide




A mixture of N-(3-bromo-2-methylphenyl)-2-(3-fluoropyridin-2-yl)acetamide (4.2 g, 13.6 mmol) and 4,4,4′,4′,5,5,5′,5′-octamethyl-2,2′-bi(1,3,2-dioxaborolane) (3.80 g, 14.9 mmol) in dioxane (40 mL) was bubbled with nitrogen for 10 min. Potassium acetate (3.33 g, 34.0 mmol) was added to the mixture, bubbling was continued for another 5 min, and PdCl2(dppf) DCM adduct (0.555 g, 0.679 mmol) was added. The mixture was heated at 100° C. overnight. The cooled mixture was diluted with EtOAc, washed sequentially with water and brine, dried and concentrated. The residue was purified by column chromatography on silica gel, eluting with DCM-methyl t-butyl ether, to provide 2-(3-fluoropyridin-2-yl)-N-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)acetamide as a white solid (3.80 g, 76% yield). Mass spectrum m/z 370 (M+H)+.
Intermediate 18:
A mixture of 2-(3-fluoropyridin-2-yl)-N-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)acetamide (9.01 g, 24.3 mmol) and CDI (15.78 g, 97 mmol) in toluene (97 mL) was heated at 120° C. for 7 h. The cooled mixture was diluted with EtOAc and washed sequentially with water and brine. The combined aqueous layers were extracted with EtOAc, and the combined organic phases were dried and concentrated. The residue was purified by column chromatography on silica gel (220 g), eluting with EtOAc-hexanes (gradient from 20-100%), to provide 5-fluoro-2-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione as a yellow solid (6.26 g, 65% yield). Mass spectrum m/z 397 (M+H)+. 1H NMR (400 MHz, chloroform-d) δ 8.11 (dd, J=7.6, 0.8 Hz, 1H), 7.94 (dd, J=7.5, 1.3 Hz, 1H), 7.35 (t, J=7.5 Hz, 1H), 7.23 (dd, J=7.8, 1.4 Hz, 1H), 6.85-6.76 (m, 1H), 6.35 (td, J=7.4, 5.0 Hz, 1H), 6.09 (s, 1H), 2.36 (s, 3H), 1.36 (s, 12H).
Intermediates 19 and 20
5-Fluoro-2-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione (single enantiomers)




Racemic 5-fluoro-2-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione [Intermediate 18] (7.50 g) was separated by chiral super-critical fluid chromatography as follows: column: CHIRALCEL® OD-H (5×25 cm, 5 μm); Mobile Phase: CO2-MeOH (76:24) at 280 mL/min, 100 bar, 40° C.; sample preparation: 62.5 mg/mL in DCM-MeOH (1:1); injection: 0.83 mL.
The first peak eluting from the column provided one enantiomer of 5-fluoro-2-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione [Intermediate 19] as a yellow solid (3.20 g, chiral purity 99.3%).
The second peak eluting from the column provided the other enantiomer of 5-fluoro-2-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione [Intermediate 20] as a yellow solid (2.98 g, chiral purity 98.6%).
The mass spectrum and 1H NMR for both enantiomers were the same as those for Intermediate 18.
Intermediate 21
3-(3-Bromo-2-methylphenyl)-1-(4-fluorophenyl)pyrimidine-2,4(1H,3H)-dione




Intermediate 21A: Methyl 3-(4-methoxybenzylamino)-2-(phenylselanyl)propanoate




A suspension of phenyl hypobromoselenoite (5.54 g, 23.5 mmol) and zinc(II) chloride (1.27 g, 9.29 mmol) in DCM (116 mL) was treated with methyl acrylate (2.1 mL, 23.2 mmol). The mixture was stirred at room temperature for 30 min, then was treated with (4-methoxyphenyl)methanamine (6.4 mL, 48.8 mmol), forming a thick suspension. After being stirred for 16 h, the precipitate was removed by filtration, washed with EtOAc, and the combined filtrates were concentrated. The residue was purified by column chromatography on silica gel (120 g), eluting with EtOAc-hexanes (gradient from 0-50%), to provide methyl 3-(4-methoxybenzylamino)-2-(phenylselanyl)propanoate as a light brown oil (3.68 g, 42% yield). Mass spectrum m/z 380 (M+H)+. 1H NMR (400 MHz, DMSO-d6) δ 7.53-7.49 (m, 2H), 7.39-7.28 (m, 3H), 7.18 (d, J=8.6 Hz, 2H), 6.88-6.82 (m, 2H), 3.89 (dd, J=8.8, 5.9 Hz, 1H), 3.73 (s, 3H), 3.61 (s, 2H), 3.55 (s, 3H), 2.93-2.78 (m, 2H).
Intermediate 21B: 1-Bromo-3-isocyanato-2-methylbenzene




A solution of triphosgene (2.25 g, 7.58 mmol) in toluene (27 mL), cooled in an ice-water bath, was treated slowly with a solution of 3-bromo-2-methylaniline (3.00 g, 16.1 mmol) and DIEA (5.6 mL, 32.2 mmol) in toluene (5.4 mL). The resulting suspension was stirred at room temperature for 2 h. The precipitate was removed by filtration and washed with EtOAc. The combined filtrates were diluted with EtOAc, washed with brine, dried and concentrated to provide 1-bromo-3-isocyanato-2-methylbenzene as a brown oil (3.68 g, 98%). 1H NMR (400 MHz, DMSO-d6) δ 7.49 (dd, J=8.1, 0.9 Hz, 1H), 7.31 (dd, J=7.9, 0.7 Hz, 1H), 7.15 (td, J=8.0, 0.7 Hz, 1H), 2.38 (s, 3H).
Intermediate 21C: 3-(3-Bromo-2-methylphenyl)-1-(4-methoxybenzyl)-5-(phenylselanyl)dihydropyrimidine-2,4(1H,3H)-dione




A mixture of methyl 3-((4-methoxybenzyl)amino)-2-(phenylselanyl)propanoate (3.68 g, 9.73 mmol), 1-bromo-3-isocyanato-2-methylbenzene (2.27 g, 10.7 mmol), and K2CO3 (0.672 g, 4.86 mmol) in DMF (49 mL) was heated at 65° C. for 5 h. The cooled mixture was partitioned between water and EtOAc. The organic phase was washed with brine, dried and concentrated to provide 3-(3-bromo-2-methylphenyl)-1-(4-methoxybenzyl)-5-(phenylselanyl)dihydropyrimidine-2,4(1H,3H)-dione as a light brown solid (5.43 g). Mass spectrum m/z 557, 559, 561 (M+H)+.
Intermediate 21D: 3-(3-Bromo-2-methylphenyl)-1-(4-methoxybenzyl)pyrimidine-2,4(1H,3H)-dione




A solution of 3-(3-bromo-2-methylphenyl)-1-(4-methoxybenzyl)-5-(phenylselanyl)dihydropyrimidine-2,4(1H,3H)-dione (5.43 g, 9.73 mmol) in THF (97 mL) was treated with 30% aqueous hydrogen peroxide (5.0 mL, 48.6 mmol) and the mixture was stirred at room temperature for 30 min. Water was added and the mixture was extracted with EtOAc. The organic phase was washed with brine, dried and concentrated. The residue was purified by column chromatography on silica gel (220 g), eluting with EtOAc-hexanes (gradient from 25-70%), to provide 3-(3-bromo-2-methylphenyl)-1-(4-methoxybenzyl)pyrimidine-2,4(1H,3H)-dione as a white solid (2.10 g, 54% yield). Mass spectrum m/z 401, 403 (M+H)+. 1H NMR (400 MHz, DMSO-d6) δ 7.95 (d, J=7.9 Hz, 1H), 7.70-7.65 (m, 1H), 7.32-7.28 (m, 2H), 7.25-7.22 (m, 2H), 6.96-6.91 (m, 2H), 5.86 (d, J=7.9 Hz, 1H), 4.89 (d, J=2.4 Hz, 2H), 3.74 (s, 3H), 2.02 (s, 3H).
Intermediate 21E: 3-(3-Bromo-2-methylphenyl)pyrimidine-2,4(1H,3H)-dione




A solution of 3-(3-bromo-2-methylphenyl)-1-(4-methoxybenzyl)pyrimidine-2,4(1H,3H)-dione (0.87 g, 2.17 mmol) in TFA (5.5 mL) was treated with trifluoromethanesulfonic acid (0.55 mL) and the mixture was stirred at room temperature overnight. The mixture was slowly poured onto ice and stirred while warming to room temperature. The precipitate was collected by filtration, washed with water and dried to provide 3-(3-bromo-2-methylphenyl)pyrimidine-2,4(1H,3H)-dione as a purple solid (0.62 g, 96% yield). Mass spectrum m/z 281, 283 (M+H)+. 1H NMR (400 MHz, DMSO-d6) δ 11.36 (d, J=4.4 Hz, 1H), 7.67 (dd, J=6.5, 2.8 Hz, 1H), 7.60 (dd, J=7.7, 5.9 Hz, 1H), 7.27-7.21 (m, 2H), 5.72 (dd, J=7.7, 1.3 Hz, 1H), 2.07 (s, 3H).
Intermediate 21:
A stirred suspension of copper(II) acetate (0.543 g, 2.99 mmol), 3-(3-bromo-2-methylphenyl)pyrimidine-2,4(1H,3H)-dione (0.42 g, 1.49 mmol), (4-fluorophenyl)boronic acid (0.418 g, 2.99 mmol), and activated molecular sieves (750 mg) in dry DCM (25 mL) was treated with pyridine (0.363 mL, 4.48 mmol) and stirred at room temperature overnight. The mixture was diluted with DCM, filtered through CELITE®, and the solids were washed with DCM and THF. The combined filtrates were washed with water, dried and concentrated. The residue was purified by column chromatography on silica gel (40 g), eluting with EtOAc-hexanes (gradient from 20-40%), to give 3-(3-bromo-2-methylphenyl)-1-(4-fluorophenyl)pyrimidine-2,4(1H,3H)-dione as a yellow glassy solid (0.36 g, 43% yield). Mass spectrum m/z 375, 377 (M+H)+. 1H NMR (400 MHz, DMSO-d6) δ 7.91 (d, J=7.9 Hz, 1H), 7.68 (dd, J=7.9, 1.3 Hz, 1H), 7.60-7.51 (m, 2H), 7.40-7.22 (m, 4H), 5.95 (d, J=7.9 Hz, 1H), 2.21-2.12 (m, 3H).
Intermediate 22
4-Bromo-7-(2-hydroxypropan-2-yl)-5-methyl-9H-carbazole-1-carboxamide




Intermediate 22A: Ethyl 3-hydroxy-5-methylbenzoate




A solution of 3-hydroxy-5-methylbenzoic acid (prepared according to the procedure of Turner et al., J. Org. Chem., 24:1952 (1959); 2.50 g, 16.4 mmol) in EtOH (100 mL) was treated with sulfuric acid (5 mL, 94 mmol) and heated to reflux on an oil bath. After 18 h the solution was cooled to room temperature. The solution was concentrated to a reduced volume (20-30 mL) and diluted with water (100-150 mL). A gum deposited which became a solid on stirring and trituration. The precipitate was collected by filtration, washed with water and dried under vacuum to provide ethyl 3-hydroxy-5-methylbenzoate as an off-white solid (2.63 g, 89% yield). Mass spectrum m/z 181 (M+H)+. 1H NMR (400 MHz, chloroform-d) δ 7.46 (d, J=0.7 Hz, 1H), 7.40 (s, 1H), 6.90 (s, 1H), 4.39 (q, J=7.0 Hz, 2H), 2.37 (s, 3H), 1.41 (t, J=7.2 Hz, 3H).
Intermediate 22B: Ethyl 3-hydroxy-5-methylcyclohexancarboxylate




A solution of ethyl 3-hydroxy-5-methylbenzoate (2.63 g, 14.6 mmol) in EtOH (50 mL) was combined with 5% rhodium on alumina (0.5 g) in a Parr pressure bottle and shaken under a hydrogen atmosphere (60 psig) at room temperature. After 21.5 h, the vessel was vented and purged with nitrogen. The mixture was filtered through CELITE® and the solids were washed with EtOH. The combined filtrates were concentrated under vacuum to provide ethyl 3-hydroxy-5-methylcyclohexanecarboxylate as a colorless oily liquid (2.64 g, 97% yield). Mass spectrum m/z 187 (M+H)+, 169 (M+H−H2O)+.
Intermediate 22C: Ethyl 3-methyl-5-oxocyclohexancarboxylate




A solution of ethyl 3-hydroxy-5-methylcyclohexanecarboxylate (2.64 g, 14.2 mmol) in acetone (45 mL) was stirred on an ice-water bath and treated dropwise with Jones reagent (4.25 mL, 14.9 mmol) until the yellow color persisted for more than 30 min. The mixture was then treated with isopropanol (ca. 2 mL) and stirred on ice until the yellow color was discharged and the mixture was a blue-green slurry. The supernatant was decanted through CELITE®. The sludge was stirred and triturated several times with fresh acetone until it was mostly a powdery solid, with the acetone washes were also filtered through CELITE®. The combined filtrates and washes were concentrated. The residue was dissolved in ether, washed with saturated brine, dried and concentrated to provide ethyl 3-methyl-5-oxocyclohexanecarboxylate as a nearly colorless oily liquid (2.275 g, 87% yield; mixture of diastereomers, ratio about 95:5). 1H NMR (major isomer) (400 MHz, chloroform-d) δ 4.18 (q, J=7.3 Hz, 2H), 2.72 (tt, J=12.7, 4.0 Hz, 1H), 2.62-2.53 (m, 1H), 2.50 (dd, J=13.1, 1.0 Hz, 1H), 2.45-2.36 (m, 1H), 2.16 (dtt, J=13.3, 3.5, 1.8 Hz, 1H), 2.07-1.97 (m, 1H), 1.97-1.82 (m, 1H), 1.57-1.42 (m, 1H), 1.29 (t, J=7.2 Hz, 3H), 1.09 (d, J=6.4 Hz, 3H).
Intermediate 22D: 5-Bromo-2-ethoxycarbonyl-4-methyl-2,3,4,9-tetrahydro-1H-carbazole-8-carboxylic acid




A suspension of 4-bromo-2-hydrazinylbenzoic acid hydrochloride [prepared according to the procedure described in U.S. Pat. No. 8,084,620, Intermediate 46-1] (605 mg, 2.26 mmol) in acetic acid (10 mL) was treated with ethyl 3-methyl-5-oxocyclohexanecarboxylate (500 mg, 2.71 mmol) and the mixture was heated at 100-105° C. After 6 h the temperature was reduced to 95° C. and stirring was continued overnight. After 22 h total the mixture was cooled to room temperature and most of the solvent was removed under vacuum. The residue was stirred in EtOAc and the mixture was filtered to remove a small amount of precipitate. The filtrate was washed with water, dried over sodium sulfate and concentrated under vacuum to give crude 5-bromo-2-(ethoxycarbonyl)-4-methyl-2,3,4,9-tetrahydro-1H-carbazole-8-carboxylic acid (mixture of diastereomers) as a dark brown gum (860 mg). Mass spectrum m/z 380, 382 (M+H)+.
Intermediate 22E: Ethyl 5-bromo-8-carbamoyl-4-methyl-2,3,4,9-tetrahydro-1H-carbazole-2-carboxylate




A solution of crude 5-bromo-2-(ethoxycarbonyl)-4-methyl-2,3,4,9-tetrahydro-1H-carbazole-8-carboxylic acid (860 mg) in THF (15 mL) was treated with 1-hydroxy-7-azabenzotriazole (369 mg, 2.71 mmol) and EDC (520 mg, 2.71 mmol) and the suspension was stirred at room temperature. After 2 h, the mixture was bubbled with anhydrous ammonia for about 2 min, forming a thick orange slurry. After 2.5 h more the mixture was diluted with water and EtOAc and the layers were separated. The aqueous phase was extracted again with EtOAc and the combined organic layers were washed sequentially with 1 M aqueous NaOH and brine, and dried and concentrated. The residue was subjected to column chromatography on silica gel (80 g), eluting with EtOAc-hexanes (gradient from 25-100%), to provide impure ethyl 5-bromo-8-carbamoyl-4-methyl-2,3,4,9-tetrahydro-1H-carbazole-2-carboxylate (mixture of diastereomers) as a yellow-tan solid (265 mg, 31% yield). Mass spectrum m/z 379, 381 (M+H)+.
Intermediate 22F: Ethyl 5-bromo-8-carbamoyl-4-methyl-9H-carbazole-2-carboxylate




A solution of impure ethyl 5-bromo-8-carbamoyl-4-methyl-2,3,4,9-tetrahydro-1H-carbazole-2-carboxylate (250 mg, 0.659 mmol) in THF (7 mL) was treated with 2,3-dichloro-5,6-dicyanobenzoquinone (329 mg, 1.45 mmol) and heated on an oil bath at 60° C. After 2.4 h the mixture was cooled to room temperature and diluted with EtOAc. The mixture was filtered and the precipitate was rinsed with EtOAc and dried under vacuum to provide a very fine white solid (71.6 mg). The filtrate was washed four times with saturated aqueous NaHCO3, then with brine. The organic phase was dried and concentrated, and the residue was sonicated in a mixture of EtOAc and MeOH. The precipitate was collected by filtration, washed with EtOAc and dried to provide a pale yellow solid (50.5 mg). This filtrate was subjected to column chromatography on silica gel (12 g), eluting with EtOAc-hexanes (gradient from 25-100%), to provide a solid which was sonicated in a minimal amount of EtOAc. The precipitate was collected by filtration, rinsed with minimal EtOAc and dried to provide a pale yellow solid (5.2 mg). The three isolated solids were combined to provide ethyl 5-bromo-8-carbamoyl-4-methyl-9H-carbazole-2-carboxylate as a pale yellow solid (127 mg, 51% yield). Mass spectrum m/z 375, 377 (M+H)+. 1H NMR (400 MHz, DMSO-d6) δ 12.17 (s, 1H), 8.42 (d, J=1.1 Hz, 1H), 8.25 (br. s., 1H), 7.88 (d, J=8.1 Hz, 1H), 7.62 (br. s., 1H), 7.59 (d, J=0.7 Hz, 1H), 7.56 (d, J=8.1 Hz, 1H), 4.36 (q, J=7.0 Hz, 2H), 3.20 (s, 3H), 1.38 (t, J=7.0 Hz, 3H).
Intermediate 22: Ethyl 4-bromo-7-(2-hydroxypropan-2-yl)-5-methyl-9H-carbazole-1-carboxamide
A suspension of ethyl 5-bromo-8-carbamoyl-4-methyl-9H-carbazole-2-carboxylate (120 mg, 0.320 mmol) in THF (2.5 mL) was stirred at −78° C. and treated dropwise with 1.6 M methyllithium in ether (0.800 mL, 1.28 mmol) over about 2.5 min. Within 0.5 h the mixture was a solid yellow mass. After 45 min from the completion of addition, THF (1 mL) was added and the mixture warmed sufficiently (still below 0° C.) to allow partial mixing, then was cooled again to −78° C. After 90 min from the completion of addition, the mixture was treated with saturated aqueous NH4Cl (2 mL) and a small amount of water, and allowed to warm to room temperature with vigorous stirring. The mixture was extracted twice with EtOAc. The combined organic phases were washed with saturated brine, dried and concentrated. The residue was subjected to column chromatography on silica gel (12 g), eluting with EtOAc-hexanes (gradient from 40-100%), to provide 4-bromo-7-(2-hydroxypropan-2-yl)-5-methyl-9H-carbazole-1-carboxamide as an off-white solid (96.3 mg, 83% yield). Mass spectrum m/z 343, 345 (M+H−H2O)+. 1H NMR (400 MHz, DMSO-d6) δ 11.73 (s, 1H), 8.19 (br. s., 1H), 7.82-7.73 (m, 2H), 7.54 (br. s., 1H), 7.45 (d, J=8.1 Hz, 1H), 7.14 (d, J=0.9 Hz, 1H), 5.03 (s, 1H), 3.14 (s, 3H), 1.50 (s, 6H).
Intermediate 23
4-Bromo-7-(2-hydroxypropan-2-yl)-8-methyl-9H-carbazole-1-carboxamide




Following the procedures used to prepare Intermediate 22, 3-hydroxy-2-methylbenzoic acid was converted into 4-bromo-7-(2-hydroxypropan-2-yl)-8-methyl-9H-carbazole-1-carboxamide. Mass spectrum m/z 343, 345 (M+H−H2O)+. 1H NMR (400 MHz, DMSO-d6) δ 10.86 (s, 1H), 8.38-8.26 (m, 2H), 7.89 (d, J=8.1 Hz, 1H), 7.67 (br. s., 1H), 7.44 (d, J=8.1 Hz, 2H), 5.09 (s, 1H), 2.81 (s, 3H), 1.63 (s, 6H).
Intermediates 24 and 24A
8-Fluoro-1-methyl(d3)-3-(S)-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)quinazoline-2,4(1H,3H)-dione (I-24), and
8-Fluoro-1-methyl(d3)-3-(R)-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)quinazoline-2,4(1H,3H-dione (I-24A)




Intermediate 24B: 8-Fluoro-1-methyl(d3)-3-(RS)-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)quinazoline-2,4(1H,3H)-dione




A mixture of 3-(3-bromo-2-methylphenyl)-8-fluoro-1-methyl(d3)quinazoline-2,4(1H,3H)-dione [Intermediate 5] (3.00 g, 8.19 mmol), 4,4,4′,4′,5,5,5′,5′-octamethyl-2,2′-bi(1,3,2-dioxaborolane) (2.70 g, 10.7 mmol) and potassium acetate (2.41 g, 24.6 mmol) in dioxane (40 mL) was bubbled with argon with sonication for ca. 2 min, then was treated with PdCl2(dppf) DCM adduct (0.335 g, 0.410 mmol). The mixture was heated at 90° C. for 15.75 h. The cooled mixture was diluted with EtOAc, filtered through CELITE®, and the solids were rinsed with EtOAc. The combined filtrates were concentrated, and the residue was purified by column chromatography on silica gel (330 g), eluting with EtOAc-hexanes (gradient from 0-40%), to give 8-fluoro-1-methyl(d3)-3-(RS)-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)quinazoline-2,4(1H,3H)-dione as an off-white solid (3.23 g, 95% yield). Mass spectrum m/z 414 (M+H)+. 1H NMR (400 MHz, chloroform-d) δ 8.14-8.07 (m, 1H), 7.93 (dd, J=7.4, 1.4 Hz, 1H), 7.48 (ddd, J=13.9, 8.1, 1.5 Hz, 1H), 7.37-7.31 (m, 1H), 7.27-7.19 (m, 2H), 2.36 (s, 3H), 1.36 (s, 12H).
Intermediates 24 and 24A:
A sample of 8-fluoro-1-methyl(d3)-3-(RS)-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)quinazoline-2,4(1H,3H)-dione [Intermediate 24B] was separated by super-critical fluid chromatography as follows: column: WHELK-O® R,R (3×25 cm, 5 μm); Mobile Phase: CO2-MeOH (70:30) at 200 mL/min, 100 bar, 30° C.; sample preparation: 3.7 mg/mL in MeOH; injection: 4.17 mL. The first peak eluting from the column provided the S enantiomer, 8-fluoro-1-methyl(d3)-3-(S)-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)quinazoline-2,4(1H,3H)-dione [Intermediate 24] as a white solid. The second peak eluting from the column provided the R enantiomer, 8-fluoro-1-methyl(d3)-3-(R)-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)quinazoline-2,4(1H,3H)-dione [Intermediate 24A] as a white solid. The mass spectrum and 1H NMR for both enantiomers were the same as those for Intermediate 24B.
Alternative Synthesis of 8-Fluoro-1-methyl(d3)-3-(S)-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)quinazoline-2,4(1H,3H)-dione [Intermediate 24]:
Intermediate 24C: 8-Fluoro-3-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)quinazoline-2,4(1H,3H)-dione




A stirred mixture of 3-(3-bromo-2-methylphenyl)-8-fluoroquinazoline-2,4(1H,3H)-dione [Intermediate 1] (0.349 g, 1.00 mmol), 4,4,4′,4′,5,5,5′,5′-octamethyl-2,2′-bi(1,3,2-dioxaborolane) (0.305 g, 1.20 mmol), PdCl2(dppf) DCM adduct (0.041 g, 0.050 mmol) and potassium acetate (0.245 g, 2.50 mmol) in dioxane (20 mL) and DMSO (4 mL) was bubbled with nitrogen for 5 min, then heated at 90° C. overnight. The mixture was cooled to room temperature and partitioned between EtOAc and water. The organic phase was washed sequentially with saturated aqueous NaHCO3 and brine, dried and concentrated. The residue was purified by column chromatography on silica gel, eluting with 20% EtOAc-hexane, to give 8-fluoro-3-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)quinazoline-2,4(1H,3H)-dione as a white solid (0.326 g, 82% yield). Mass Spectrum m/z 397 (M+H)+. 1H NMR (500 MHz, DMSO-d6) δ 11.78 (s, 1H), 7.80 (d, J=7.2 Hz, 1H), 7.72 (dd, J=7.4, 1.5 Hz, 1H), 7.71-7.56 (m, 1H), 7.45-7.35 (m, 1H), 7.35-7.29 (m, 1H), 7.29-7.16 (m, 1H), 2.22 (s, 3H), 1.33 (s, 12H).
Intermediate 24D: 8-Fluoro-3-(S)-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)quinazoline-2,4(1H,3H)-dione




A sample of 8-fluoro-3-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)quinazoline-2,4(1H,3H)-dione [Intermediate 24C] was separated by chiral super-critical fluid chromatography as follows: column: CHIRALCEL® OD-H (5×25 cm, 5 μm); Mobile Phase: CO2-MeOH (70:30) at 300 mL/min, 100 bar, 40° C.; sample preparation: 103 mg/mL in DCM-MeOH (44:56); injection: 5.0 mL. The second peak eluting from the column gave the S enantiomer, 8-fluoro-3-(S)-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)quinazoline-2,4(1H,3H)-dione, as a white solid. Mass spectrum m/z 397 (M+H)+. 1H NMR (400 MHz, chloroform-d) δ 8.19 (s, 1H), 7.99 (d, J=8.1 Hz, 1H), 7.95 (dd, J=7.3, 1.3 Hz, 1H), 7.46 (ddd, J=9.8, 8.3, 1.2 Hz, 1H), 7.39-7.32 (m, 1H), 7.28-7.18 (m, 2H), 2.39 (s, 3H), 1.36 (s, 12H).
Intermediate 24:
A solution of 8-fluoro-3-(S)-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)quinazoline-2,4(1H,3H)-dione [Intermediate 24D] (5.42 g, 13.7 mmol) in THF (100 mL) was stirred on an ice-water bath and treated with Cs2CO3 (6.24 g, 19.2 mmol), then with iodomethane-d3 (1.02 mL, 16.4 mmol) and the mixture was stirred at room temperature for 16.25 h. The mixture was filtered, the solid was rinsed with EtOAc, and the combined filtrates were concentrated. The residue was dissolved in EtOAc and washed sequentially with water and brine, dried and concentrated to provide 8-fluoro-1-methyl(d3)-3-(S)-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)quinazoline-2,4(1H,3H)-dione as a white solid (5.538 g, 98% yield). Mass spectrum m/z 414 (M+H)+. 1H NMR (400 MHz, chloroform-d) δ 8.11 (dq, J=7.8, 0.8 Hz, 1H), 7.93 (dd, J=7.5, 1.3 Hz, 1H), 7.48 (ddd, J=13.9, 8.1, 1.5 Hz, 1H), 7.38-7.30 (m, 1H), 7.27-7.20 (m, 2H), 2.36 (s, 3H), 1.36 (s, 12H).
Intermediate 25
4-Bromo-7-(methoxymethyl)-9H-carbazole-1-carboxamide




A stirred suspension of 4-bromo-7-(hydroxymethyl)-9H-carbazole-1-carboxamide [prepared according to the procedure described in U.S. Pat. No. 8,084,620, Example 30-02] (0.5 g, 1.57 mmol) in THF (10 mL) was treated dropwise with thionyl chloride (0.252 mL, 3.45 mmol) and the mixture was stirred at room temperature for 1 h. The mixture was treated with MeOH (0.2 mL), and the solvent and excess thionyl chloride were removed under vacuum. The residue was dissolved in MeOH (10 mL), and sodium methoxide (0.423 g, 7.83 mmol) was added. The mixture was stirred overnight at room temperature. The mixture was diluted with EtOAc and washed sequentially with water and brine. The combined aqueous layers were extracted with EtOAc, and the combined organic phases were dried and concentrated. The residue was purified by column chromatography on silica gel (40 g), eluting with EtOAc-hexanes (gradient from 0-100%), to give 4-bromo-7-(methoxymethyl)-9H-carbazole-1-carboxamide as a white solid (119 mg, 23% yield). Mass spectrum m/z 333, 335 (M+H)+.
Examples 1 and 2
4-(3-(8-Fluoro-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (Mixtures of Two Interconverting diastereomers)




Preparation 1A: 4-(3-(8-Fluoro-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (Mixture of Four diastereomers)
A mixture of 7-(2-hydroxypropan-2-yl)-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-9H-carbazole-1-carboxamide (43.4 mg, 0.110 mmol) [Intermediate 4], 3-(3-bromo-2-methylphenyl)-8-fluoroquinazoline-2,4(1H,3H)-dione (32 mg, 0.092 mmol) [Intermediate 1], tetrakis(triphenylphosphine)palladium (5.3 mg, 4.58 μmol), and potassium carbonate (38.0 mg, 0.275 mmol) in THF (3 mL) was sealed in a vial and heated at 90° C. overnight. The cooled mixture was purified by column chromatography on silica gel (40 g), eluting with DCM-MeOH—NH4OH (gradient from 90:9:1 to 97:2.7:0.3), to give 4-(3-(8-fluoro-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (mixture of four diastereomers) as a white solid (6 mg, 13% yield. Mass spectrum m/z 519 (M+H−H2O)+.
1H NMR (500 MHz, chloroform-d/MeOH-d4) δ 7.70 (d, J=1.5 Hz, 1H), 7.62-7.58 (m, 1H), 7.55-7.47 (m, 2H), 7.46-7.42 (m, 1H), 7.38 (d, J=7.9 Hz, 1H), 7.28-7.19 (m, 3H), 7.14 (dd, J=8.4, 1.5 Hz, 1H), 7.10 (dd, J=10.9, 7.9 Hz, 1H), 1.89 (s, 3H), 1.61 (dd, J=10.2, 3.2 Hz, 6H).
1H NMR (400 MHz, DMSO-d6) δ 11.85 (s, 0.5H), 11.79 (s, 0.5H), 11.39 (s, 1H), 8.16 (br. s., 1H), 7.99 (d, J=7.7 Hz, 1H), 7.90-7.79 (m, 2H), 7.69-7.61 (m, 1H), 7.53-7.42 (m, 3H), 7.35 (dt, J=6.0, 3.3 Hz, 1H), 7.28-7.20 (m, 1H), 7.11-7.06 (m, 1H), 7.03 (t, J=7.5 Hz, 2H), 4.98 (s, 0.5H), 4.96 (s, 0.5H), 1.76 (s, 3H), 1.48-1.42 (m, 6H).
19F NMR (376 MHz, DMSO-d6) δ −129.97 (s, 1F), −130.02 (s, 1F).
Examples 1 and 2
A sample of 4-(3-(8-fluoro-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (mixture of four diastereomers) (1.2 g) was separated by chiral super-critical fluid chromatography as follows: column: CHIRALCEL® OD-H (3×25 cm, 5 μm); Mobile Phase: CO2-(1:1 MeOH-acetonitrile) (45:55) at 145 mL/min, 100 bar, 40° C.; sample preparation: 50 mg/mL in DMSO-MeOH (1:1); injection: 3.5 mL.
The first peak eluting from the column provided one pair of interconverting diastereomers of 4-(3-(8-fluoro-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide [Example 1] as a white solid (603 mg).
The second peak eluting from the column provided the other pair of interconverting diastereomers 4-(3-(8-fluoro-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide [Example 2] as a white solid (584 mg).
The mass spectra and NMR spectra for both were the same as those for the mixture of four diastereomers shown above. The absolute stereochemistry of Examples 1 and 2 has not been assigned.
Example 3
4-(3-(S)-(8-Fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (Mixture of Two Interconverting diastereomers)




Preparation 3A: 4-(3-(8-Fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (Mixture of Four diastereomers)




A mixture of 4-bromo-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide [synthesized according to the procedure described in U.S. Pat. No. 8,084,620, Intermediate 73-2] (827 mg, 2.38 mmol), 8-fluoro-1-methyl-3-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)quinazoline-2,4(1H,3H)-dione [Intermediate 2] (850 mg, 2.07 mmol), 2 M aqueous tripotassium phosphate (3.11 mL, 6.22 mmol), and tetrakis(triphenylphosphine)palladium (120 mg, 0.104 mmol) in a mixture of toluene (27 mL) and EtOH (9 mL) was heated at reflux under nitrogen overnight. The mixture was cooled to room temperature, diluted with EtOAc, washed with water, dried and concentrated. The residue was subjected to column chromatography on silica gel (330 g), eluting with DCM-MeOH—NH4OH (gradient from 90:9:1 to 97:2.7:0.3), to give 4-(3-(8-fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (mixture of four diastereomers) as a white solid (860 mg, 74% yield). Mass spectrum m/z 533 (M+H−H2O)+. 1H NMR (400 MHz, chloroform-d) δ 10.56 (s, 1H), 8.15 (dd, J=9.8, 8.7 Hz, 1H), 7.71-7.64 (m, 2H), 7.54-7.42 (m, 3H), 7.36 (dd, J=7.7, 1.3 Hz, 1H), 7.28-7.24 (m, 3H), 7.18-7.11 (m, 1H), 6.02 (br. s., 2H), 3.91 (dd, J=7.9, 2.9 Hz, 3H), 1.90 (s, 3H), 1.85 (d, J=4.0 Hz, 1H), 1.67 (d, J=1.3 Hz, 6H). 1H NMR (400 MHz, DMSO-d6) δ 11.40 (s, 1H), 8.17 (br. s., 1H), 8.04-7.93 (m, 2H), 7.84 (d, J=1.1 Hz, 1H), 7.74 (ddd, J=14.4, 8.0, 1.3 Hz, 1H), 7.54-7.42 (m, 3H), 7.38-7.30 (m, 2H), 7.12-7.05 (m, 1H), 7.04-6.99 (m, 2H), 4.99 (d, J=1.3 Hz, 1H), 3.75 (t, J=8.4 Hz, 3H), 1.76 (s, 3H), 1.50-1.45 (m, 6H).
Alternative Preparation 3A:
A mixture of 4-(3-(8-fluoro-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (mixture of four diastereomers) [Preparation 1A] (20 mg, 0.037 mmol), iodomethane (0.15 mL of a 35 mg/mL solution in DMF; 5.29 mg, 0.037 mmol) and Cs2CO3 (12.1 mg, 0.037 mmol) in DMF (2 mL) was stirred at room temperature for 17 h. The mixture was diluted with EtOAc, the organics were washed sequentially with water and 10% aqueous LiCl, and dried and concentrated. The residue was purified by column chromatography on silica gel (12 g), eluting with DCM:MeOH:NH4OH (gradient from 90:9:1 to 97:2.7:0.3) to give 4-(3-(8-fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (mixture of four diastereomers) as a white solid (19 mg, 93% yield). Mass spectrum m/z 533 (M+H−H2O)+. 1H NMR (400 MHz, chloroform-d) δ 10.56 (s, 1H), 8.14 (dd, J=9.6, 8.5 Hz, 1H), 8.04 (s, 1H), 7.70-7.63 (m, 2H), 7.54-7.41 (m, 3H), 7.35 (dd, J=7.8, 1.2 Hz, 1H), 7.28-7.22 (m, 3H), 7.14 (dd, J=7.8, 1.7 Hz, 1H), 3.90 (dd, J=7.9, 2.9 Hz, 3H), 1.90 (s, 3H), 1.66 (d, J=1.3 Hz, 6H).
Example 3
A sample of 4-(3-(8-fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (mixture of four diastereomers) (532 mg, 0.966 mmol) was separated by chiral super-critical fluid chromatography as follows: column: Lux Cel-4 (3×25 cm, 5 μm); Mobile Phase: CO2-MeOH (60:40) at 85 mL/min; sample preparation: 6.7 mg/mL in MeOH-acetone (9:1); injection: 3 mL.
Peaks 3 and 4 eluting from the column were collected together and concentrated to give 4-(3-(S)-(8-fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (mixture of two interconverting diastereomers) as a light yellow solid (230 mg).
Mass spectrum m/z 533 (M+H−H2O)+. 1H NMR (400 MHz, DMSO-d6) δ 11.40 (s, 1H), 8.16 (br. s., 1H), 8.04-7.93 (m, 2H), 7.84 (s, 1H), 7.78-7.69 (m, 1H), 7.55-7.43 (m, 3H), 7.39-7.30 (m, 2H), 7.13-6.98 (m, 3H), 4.99 (d, J=1.1 Hz, 1H), 3.75 (t, J=8.4 Hz, 3H), 1.75 (s, 3H), 1.47 (d, J=4.4 Hz, 6H).
Alternative Synthesis of Example 3:
A suspension of 8-fluoro-1-methyl-3-(S)(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)quinazoline-2,4(1H,3H)-dione [Intermediate 3] (13.00 g, 31.7 mmol), 4-bromo-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide [synthesized according to the procedure described in U.S. Pat. No. 8,084,620, Intermediate 73-2, and recrystallized from MeOH] (10.00 g, 28.8 mmol), Cs2CO3 (18.77 g, 57.6 mmol), THF (120 mL) and water (30 mL) was bubbled with nitrogen for 5 min, then was treated with PdCl2(dppf) DCM adduct (1.11 g, 1.44 mmol). The mixture was heated at 40° C. for 24 h, then cooled to room temperature. The mixture was filtered through a pad of CELITE® and the solids were washed with EtOAc. The combined filtrates were washed with brine, dried and concentrated. The residue was purified by column chromatography on silica gel (330 g), eluting with a gradient from DCM to EtOAc, to give a solid. Recrystallization from acetone provided 4-(3-(S)-(8-fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (mixture of two interconverting diastereomers) as a crystalline solid (6.80 g, 86% yield). 1H NMR (500 MHz, DMSO-d6) δ 11.40 (s, 1H), 8.17 (br. s., 1H), 8.00 (d, J=7.7 Hz, 2H), 7.95 (d, J=7.7 Hz, 1H), 7.84 (s, 1H), 7.72 (dd, J=14.3, 8.1 Hz, 1H), 7.52-7.47 (m, 1H), 7.50-7.44 (m, 1H), 7.47-7.42 (m, 1H), 7.38-7.34 (m, 1H), 7.35-7.29 (m, 1H), 7.12-7.05 (m, 1H), 7.04-7.00 (m, 1H), 7.04-6.98 (m, 1H), 4.99 (s, 1H), 3.74 (dd, J=11.9, 8.1 Hz, 3H), 1.75 (s, 3H), 1.47 (s, 3H), 1.46 (s, 3H).
The absolute stereochemistries of the two interconverting diastereomers of Example 3 are shown in FIG. 2. The stereochemistry of Example 3 was confirmed by small crystal x-ray analysis. Several crystal forms were obtained.
Crystal Form SA-1 was prepared by the slow evaporation of an aqueous methanol solution at room temperature. Form SA-1 is a mixed solvate crystal having a stoichiometry of one molecule of methanol and one molecule of water for each molecule of Example 3.
Crystal Form SB-2 was prepared from a solution of EtOH/racemic propylene glycol. Form SB-2 is a mixed solvate crystal having a stoichiometry of one molecule of 1-(S)-propylene glycol and 0.5 molecule of water for each molecule of Example 3.
Crystal Form SC-3 was prepared by the slow evaporation of an acetone solution at room temperature. Form SC-3 is a mixed solvate crystal having a stoichiometry of one molecule of acetone and one molecule of water for each molecule of Example 3.
Crystal Form SD-3 was prepared a THF slurry. Form SD-3 is a mixed solvate crystal having a stoichiometry of 1.5 molecule of THF and one molecule of water for each molecule of Example 3.
Crystal Form SE-2 was prepared by the slow evaporation of an EtOH/THF solution at room temperature. Form SE-2 is a mixed solvate crystal having a stoichiometry of one molecule of ethanol and 0.5 molecule of water for each molecule of Example 3.
Crystal Form M2-4 was prepared by the slow evaporation of a methanol solution at room temperature. Form M2-4 is a dimethanolate crystal.
Crystal Form AN-5 was prepared by the slow evaporation of acetonitrile solution at room temperature. Form AN-5 contains acetonitrile.
Crystal Form H1-6 was prepared from a n-BuOAc slurry. Form H1-6 is a monohydrate crystal.
Crystal Form E-7 was prepared by the slow evaporation of an EtOH/THF solution at room temperature. Form E-7 contains ethanol.
Crystal Form SE-8 was prepared by the slow evaporation of an EtOH/THF solution at room temperature. Form SE-8 is a mixed solvate crystal.
Unit cell parameters of these crystal forms are shown in Table 4, and fractional atomic coordinates of these crystal forms are shown in Tables 5 through 14.
Example 4
4-(3-(S)-(8-Fluoro-1-methyl(d3)-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (Mixture of Two Interconverting diastereomers)




Preparation 4A: 4-(3-(8-Fluoro-1-methyl(d3)-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (Mixture of Four diastereomers)




A mixture of 7-(2-hydroxypropan-2-yl)-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-9H-carbazole-1-carboxamide [Intermediate 4] (53.8 mg, 0.137 mmol), 3-(3-bromo-2-methylphenyl)-8-fluoro-1-methyl(d3)quinazoline-2,4(1H,3H)-dione [Intermediate 5] (50 mg, 0.137 mmol), and K2CO3 (56.6 mg, 0.410 mmol) in toluene (1.2 mL) and EtOH (0.4 mL) was bubbled with argon for about 5 min (1 min with sonication). The mixture was treated with tetrakis(triphenylphosphine)palladium (7.9 mg, 6.83 μmol), heated at 90° C. for 16.25 h, and cooled to room temperature. The mixture was concentrated, and the residue was sonicated in MeOH, filtered, and purified by preparative HPLC (PHENOMENEX® Axia C18 30×100 mm, 10-100% acetonitrile-water containing 0.1% trifluoroacetic acid, 10 min, 30 mL/min, 254 nm) in 5 injections. The appropriate fractions were combined, treated with saturated aqueous NaHCO3 and concentrated under vacuum to an aqueous suspension. The precipitate was collected by filtration, washed with water and dried under vacuum to provide 4-(3-(8-fluoro-1-methyl(d3)-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (mixture of four diastercomers) as a white solid (24.3 mg, 31% yield). Mass spectrum m/z 536 (M+H−H2O)+. 1H NMR (400 MHz, DMSO-d6) δ 11.40 (s, 1H), 8.17 (br. s., 1H), 8.04-7.92 (m, 2H), 7.84 (s, 1H), 7.79-7.67 (m, 1H), 7.55-7.42 (m, 3H), 7.40-7.29 (m, 2H), 7.13-6.97 (m, 3H), 4.99 (s, 1H), 1.76 (s, 3H), 1.53-1.42 (2s, 6H).
Example 4
4-(3-(8-Fluoro-1-methyl(d3)-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (mixture of four diastereomers) (86 mg) was separated by chiral super-critical fluid chromatography as follows: column: Lux Cel-4 (3×25 cm, 5 μm); Mobile Phase: CO2-MeOH (60:40) at 85 mL/min; sample preparation: 1.3 mg/mL in MeOH-MeCN; injection: 3 mL. Peaks 3 and 4 eluting from the column were collected together and concentrated to give 4-(3-(S)-(8-fluoro-1-methyl(d3)-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (mixture of two interconverting diastereomers) (27 mg) as a white solid. Mass spectrum and NMR were identical to those of the mixture of four diastereomers.
Example 5
4-(2-Chloro-3-(8-fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)phenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (Mixture of Four diastereomers)




A mixture of 7-(2-hydroxypropan-2-yl)-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-9H-carbazole-1-carboxamide [Intermediate 4] (50 mg, 0.127 mmol), 3-(3-bromo-2-chlorophenyl)-8-fluoro-1-methylquinazoline-2,4(1H,3H)-dione [Intermediate 9] (48.6 mg, 0.127 mmol), EtOH (1 mL), toluene (1 mL) and 2 M aqueous Na2CO3 (0.21 mL, 0.418 mmol) was bubbled with nitrogen for 5 min and treated with tetrakis(triphenylphosphine)palladium (11.7 mg, 10.2 μmol). The mixture was heated at 90° C. for 16 h, cooled to room temperature and partitioned between EtOAc and water. The organic phase was dried and concentrated, and the residue was purified by column chromatography on silica gel (12 g), eluting with MeOH-DCM containing 1% triethylamine (gradient from 0-5%). The resulting material was further purified using preparative HPLC (PHENOMENEX® Axia C18 30×100 mm), eluting with MeCN-water containing 0.1% trifluoroacetic acid (gradient from 20-100%, 10 min, 30 mL/min). The appropriate fractions were combined, treated with saturated aqueous NaHCO3 and concentrated. The residue was dissolved in EtOAc, washed sequentially with water and brine, dried and concentrated to provide 4-(2-chloro-3-(8-fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)phenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (mixture of four diastereomers) as a white solid (6 mg, 8% yield). Mass spectrum m/z 571 (M+H)+. 1H NMR (400 MHz, chloroform-d) δ 0.55 (s, 1H), 8.13 (t, J=7.5 Hz, 1H), 7.78-7.38 (m, 6H), 7.20-7.08 (m, 1H), 3.90 (s, 1.5H), 3.88 (s, 1.5H), 1.65 (s, 6H). 19F NMR (400 MHz, chloroform-d) δ 121.34 ppm.
Example 6
4-(2-Chloro-3-(1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)phenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (Mixture of Four diastereomers)




A mixture of 7-(2-hydroxypropan-2-yl)-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-9H-carbazole-1-carboxamide [Intermediate 4] (140 mg, 0.356 mmol), (Z)-4-((3-bromo-2-chlorophenyl)imino)-1-methyl-1H-benzo[d][1,3]oxazin-2(4H)-one [Intermediate 8] (100 mg, 0.274 mmol), K2CO3 (151 mg, 1.09 mmol), toluene (3 mL) and EtOH (3 mL) was bubbled with nitrogen for 5 min, and treated with tetrakis(triphenylphosphine)palladium (32 mg, 0.027 mmol). The mixture was heated at 90° C., then was cooled to room temperature and concentrated. The residue was partitioned between water and EtOAc, and the aqueous phase was extracted three times with EtOAc. The combined organic phases were washed with brine, dried and concentrated. The residue was purified by column chromatography on silica gel (80 g), eluting with MeOH-DCM containing 0.5% NH4OH (gradient from 0-10%), to provide a white solid. This was further purified by preparative HPLC, eluting with MeCN-water containing TFA (gradient from 30-100%). The combined fractions containing the product were treated with saturated aqueous NaHCO3 and concentrated. The aqueous residue was extracted 3 times with EtOAc, and the combined organic phases were washed with brine, dried and concentrated to provide 4-(2-chloro-3-(1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)phenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (mixture of four diastereomers) as an off-white solid (50 mg, 32% yield). Mass spectrum m/z 535, 537 (M+H−H2O)+. 1H NMR (400 MHz, DMSO-d6) δ 11.40 (s, 1H), 8.20-8.16 (m, 1H), 8.16 (d, J=1.3 Hz, 0.5H), 8.12-8.08 (m, 0.5H), 7.98 (d, J=7.9 Hz, 1H), 7.90-7.86 (m, 0.5H), 7.85-7.82 (m, 1.5H), 7.74-7.70 (m, 1H), 7.69-7.63 (m, 1H), 7.58-7.53 (m, 2H), 7.48 (br. s., 1H), 7.41-7.34 (m, 1H), 7.13-6.99 (m, 3H), 4.99 (s, 0.5H), 4.99 (s, 0.5H), 3.61 (s, 1.5H), 3.59 (s, 1.5H), 1.47 (m, 6H).
Example 7
7-(2-Hydroxypropan-2-yl)-4-(3-(8-methoxy-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-9H-carbazole-1-carboxamide (Mixture of Four diastereomers)




A mixture of 7-(2-hydroxypropan-2-yl)-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-9H-carbazole-1-carboxamide [Intermediate 4] (42 mg, 0.107 mmol), 3-(3-bromo-2-methylphenyl)-8-methoxy-1-methylquinazoline-2,4(1H,3H)-dione [Intermediate 6] (40 mg, 0.107 mmol), PdCl2(dppf) DCM adduct (8.7 mg, 10.7 μmol) and Cs2CO3 (70 mg, 0.213 mmol) in THF (2 mL) and water (0.5 mL) in a pressure reaction vial was heated at 70° C. After 2 h, additional 7-(2-hydroxypropan-2-yl)-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-9H-carbazole-1-carboxamide (20 mg, 0.051 mmol) was added, and heating was continued for 6 h more. The mixture was cooled to room temperature, filtered, and the organic phase of the filtrate was separated and concentrated. The residue was purified by column chromatography on silica gel (40 g), eluting with EtOAc, to give 7-(2-hydroxypropan-2-yl)-4-(3-(8-methoxy-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-9H-carbazole-1-carboxamide (mixture of four diastereomers) (54 mg, 85% yield). Mass spectrum m/z 545 (M−H2O+H)+, 585 (M+Na)+. 1H NMR (400 MHz, chloroform-d) δ 10.55 (s, 1H), 7.95 (ddd, J=10.2, 6.7, 2.6 Hz, 1H), 7.69 (s, 1H), 7.63 (dd, J=7.8, 1.0 Hz, 1H), 7.52-7.46 (m, 1H), 7.38 (dd, J=17.4, 7.7 Hz, 2H), 7.28-7.24 (m, 4H), 7.10 (dd, J=7.7, 2.0 Hz, 1H), 3.96 (d, J=0.9 Hz, 3H), 3.92 (d, J=1.8 Hz, 3H), 1.88 (s, 3H), 1.66 (d, J=1.8 Hz, 6H).
Example 8
4-(3-(6-Fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (Mixture of Four diastereomers)




A mixture of 7-(2-hydroxypropan-2-yl)-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-9H-carbazole-1-carboxamide [Intermediate 4] (40 mg, 0.101 mmol), 3-(3-bromo-2-methylphenyl)-6-fluoro-1-methylquinazoline-2,4(1H,3H)-dione [Intermediate 7] (37 mg, 0.101 mmol), tetrakis(triphenylphosphine)palladium (5.9 mg, 5.07 μmol) and 2 M aqueous tripotassium phosphate (0.101 mL, 0.203 mmol) in THF (2 mL) was heated at 110° C. for 10 h. The mixture was cooled to room temperature and the organic phase was separated and concentrated. The residue was purified by preparative HPLC (Waters XBridge C18, 19×150 mm, 5-μm), eluting with MeOH-water containing 10 mM ammonium acetate (gradient from 5-95%, 20 mL/min). Fractions containing the product were combined and dried via centrifugal evaporation. The material was further purified by preparative HPLC (Waters XBridge C18, 19×250 mm, 5-μm), eluting with MeCN-water containing 10 mM ammonium acetate (gradient from 5-95%, 20 mL/min). Fractions containing the desired product were combined and dried via centrifugal evaporation to give 4-(3-(6-fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (mixture of four diastereomers) (15 mg, 27% yield). Mass spectrum m/z 533 (M−H2O+H)+. 1H NMR (500 MHz, DMSO-d6) δ 11.42 (1H, s), 8.19 (1H, br. s.), 8.00 (1H, d, J=7.43 Hz), 7.74-7.90 (3H, m), 7.62 (1H, dd, J=9.41, 4.46 Hz), 7.43-7.53 (3H, m), 7.36 (1H, d, J=5.95 Hz), 6.98-7.11 (3H, m), 3.57-3.64 (3H, m), 1.74 (3H, s), 1.43-1.51 (6H, m).
Example 9
4-(3-(3-(4-Fluorophenyl)-2,6-dioxo-2,3-dihydropyrimidin-1(6H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (Mixture of Four diastereomers)




A mixture of 7-(2-hydroxypropan-2-yl)-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-9H-carbazole-1-carboxamide [Intermediate 4] (100 mg, 0.254 mmol), 3-(3-bromo-2-methylphenyl)-1-(4-fluorophenyl)pyrimidine-2,4(1H,3H)-dione [Intermediate 21] (100 mg, 0.266 mmol), Cs2CO3 (165 mg, 0.507 mmol) and PdCl2(dppf) DCM adduct (20.7 mg, 0.025 mmol) in THF (5.0 mL) and water (1.3 mL) was heated at 45° C. for 17 h. Heating was increased to 80° C. for 2 h, then to 85° C. for 18 h. The mixture was cooled, concentrated, dissolved in DMF-MeOH and purified by preparative HPLC. The appropriate fractions were combined, treated with solid NaHCO3, and concentrated to an aqueous suspension. The precipitate was collected by filtration, washed with water and dried. The filtrates were concentrated to provide additional precipitate which was collected by filtration, washed with water and dried. The two precipitates were combined to give 4-(3-(3-(4-fluorophenyl)-2,6-dioxo-2,3-dihydropyrimidin-1(6H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (mixture of four diastereomers) as a white solid (59 mg, 41% yield). Mass spectrum m/z 545 (M+H−H2O)+. 1H NMR (400 MHz, DMSO-d6) δ 11.38 (d, J=4.8 Hz, 1H), 8.16 (br. s., 1H), 7.98 (dd, J=7.7, 2.9 Hz, 1H), 7.91 (dd, J=7.9, 1.1 Hz, 1H), 7.82 (d, J=10.1 Hz, 1H), 7.58 (ddd, J=9.1, 4.9, 2.3 Hz, 2H), 7.51-7.40 (m, 3H), 7.39-7.28 (m, 3H), 7.04 (s, 1H), 6.99 (t, J=7.4 Hz, 1H), 6.92-6.82 (m, 1H), 5.98 (dd, J=16.1, 7.9 Hz, 1H), 4.98-4.90 (m, 1H), 1.79 (d, J=4.0 Hz, 3H), 1.49-1.36 (m, 6H).
Example 10
7-(2-Hydroxypropan-2-yl)-4-(3-(7-methoxy-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-9H-carbazole-1-carboxamide (Mixture of Four diastereomers)




A mixture of 7-(2-hydroxypropan-2-yl)-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-9H-carbazole-1-carboxamide [Intermediate 4] (26.4 mg, 0.067 mmol), 2-(3-bromo-2-methylphenyl)-7-methoxy-1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione [Intermediate 12] (22 mg, 0.061 mmol) and 2 M aqueous Na2CO3 (0.076 mL, 0.152 mmol) in DMF (1.5 mL) was bubbled with argon for 5 min, then treated with PdCl2(dppf) DCM adduct (2.5 mg, 3.05 μmol). After bubbling with argon for 30 sec more, the vial was sealed and the mixture was stirred at 90° C. for 4 h. The cooled mixture was partitioned between EtOAc and water, and the organic layer was washed with brine, dried and concentrated. The residue was purified by column chromatography on silica gel (24 g), eluting with EtOAc-hexanes (sequentially 85%, 95% and 100%), to give 7-(2-hydroxypropan-2-yl)-4-(3-(7-methoxy-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-9H-carbazole-1-carboxamide (mixture of four diastereomers) as a yellow solid (11.6 mg, 34% yield). Mass spectrum m/z 531 (M+H−H2O)+. 1H NMR (400 MHz, chloroform-d) δ 10.53 (d, J=7.7 Hz, 1H), 7.77 (d, J=16.5 Hz, 1H), 7.69-7.63 (m, 2H), 7.54-7.48 (m, 1H), 7.45-7.33 (m, 2H), 7.26-7.20 (m, 1H), 7.15 (dd, J=13.4, 7.9 Hz, 1H), 6.98-6.92 (m, 1H), 6.91-6.87 (m, 1H), 5.84 (d, J=2.2 Hz, 1H), 3.81 (s, 3H), 1.89 (s, 3H), 1.55 (s, 6H).
Example 11
7-(2-Hydroxypropan-2-yl)-4-(3-(6-methoxy-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-9H-carbazole-1-carboxamide (Mixture of Four diastereomers)




A mixture of 7-(2-hydroxypropan-2-yl)-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-9H-carbazole-1-carboxamide [Intermediate 4] (48 mg, 0.122 mmol), 2-(3-bromo-2-methylphenyl)-6-methoxy-1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione [Intermediate 11] (40 mg, 0.111 mmol) and Cs2CO3 (72 mg, 0.221 mmol) in THF (1.5 mL) and water (0.375 mL) was bubbled with argon for 3 min, then treated with PdCl2(dppf) DCM adduct (4.5 mg, 5.54 μmol). Bubbling with argon was continued for 1 min and the mixture was then heated at 45° C. After 3.5 h, the mixture was cooled, diluted with EtOAc, washed sequentially with water and brine, and the combined aqueous layers were extracted with EtOAc. The combined organic phases were dried and concentrated. The residue was purified by column chromatography on silica gel (24 g), eluting with EtOAc, to give an impure material. The residue was purified further by preparative HPLC. The appropriate fractions were treated with saturated aqueous NaHCO3, combined and extracted with EtOAc. The organic phase was washed with brine, dried and concentrated to give 7-(2-hydroxypropan-2-yl)-4-(3-(6-methoxy-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-9H-carbazole-1-carboxamide (mixture of four diastereomers) as a yellow solid (17.7 mg, 28% yield). Mass spectrum 549 (M+H)+. 1H NMR (400 MHz, chloroform-d) δ 10.53 (d, J=7.3 Hz, 1H), 8.28 (dd, J=19.4, 8.1 Hz, 1H), 7.69-7.62 (m, 2H), 7.52-7.47 (m, 1H), 7.41 (dd, J=3.7, 1.3 Hz, 1H), 7.37-7.34 (m, 1H), 7.24-7.21 (m, 1H), 7.13 (dd, J=10.3, 7.7 Hz, 1H), 6.19 (ddd, J=8.0, 4.5, 2.6 Hz, 1H), 6.06 (d, J=2.4 Hz, 1H), 5.67 (d, J=2.0 Hz, 1H), 3.90-3.89 (m, 3H), 1.89-1.88 (m, 3H), 1.65-1.62 (m, 6H).
Example 12
7-(2-Hydroxypropan-2-yl)-4-(3-(5-methoxy-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-9H-carbazole-1-carboxamide (Mixture of Four diastereomers)




A mixture of 7-(2-hydroxypropan-2-yl)-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-9H-carbazole-1-carboxamide [Intermediate 4] (88 mg, 0.222 mmol), 2-(3-bromo-2-methylphenyl)-5-methoxy-1H-pyrido[1,2-e]pyrimidine-1,3(2H)-dione [Intermediate 10] (73 mg, 0.202 mmol) and 2 M aqueous Na2CO3 (0.252 mL, 0.505 mmol) in DMF (1.5 mL) at room temperature was bubbled with argon for 5 min. PdCl2(dppf) DCM adduct (8.2 mg, 10.1 μmol) was added, bubbling with nitrogen was continued for another 30 sec, and the mixture was heated at 90° C. After 4 h, the cooled mixture was diluted with EtOAc and washed sequentially with water and brine. The combined aqueous layers were extracted with EtOAc. The combined organic phases were dried and concentrated. The residue was purified by column chromatography on silica gel (40 g), eluting with EtOAc, to give a brownish solid. This was triturated in MeOH with sonication, collected by filtration and dried to provide 7-(2-hydroxypropan-2-yl)-4-(3-(5-methoxy-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-9H-carbazole-1-carboxamide (mixture of four diastereomers) as a light yellow-tan solid (29.7 mg, 25% yield). Mass spectrum 549 (M+H)+. 1H NMR (400 MHz, DMSO-d6) δ 11.38 (s, 1H), 8.15 (br. s., 1H), 7.98 (d, J=7.9 Hz, 1H), 7.91 (d, J=7.5 Hz, 1H), 7.82 (s, 1H), 7.53-7.42 (m, 3H), 7.34 (d, J=7.3 Hz, 1H), 7.07-6.98 (m, 3H), 6.71 (d, J=7.5 Hz, 1H), 6.60-6.54 (m, 1H), 5.93 (s, 1H), 4.97 (s, 1H), 3.91 (s, 3H), 1.73 (s, 3H), 1.48-1.43 (m, 6H).
Example 13
4-(3-(5-Chloro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (Mixture of Four diastereomers)




A mixture of 4-bromo-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide [synthesized according to the procedure described in U.S. Pat. No. 8,084,620, Intermediate 73-2] (23.1 mg, 0.067 mmol), 5-chloro-2-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione (25 mg, 0.061 mmol) [Intermediate 13] and Cs2CO3 (39.5 mg, 0.121 mmol) in THF (1.5 mL) and water (0.375 mL) was bubbled with argon for 3 min. The mixture was treated PdCl2(dppf) DCM adduct (2.5 mg, 3.03 μmol) and bubbling was continued for 1 min. The mixture was heated at 45° C. for 3.5 h. The cooled mixture was diluted with EtOAc, washed sequentially with water and brine, and the combined aqueous layers were extracted with EtOAc. The combined organic phases were dried and concentrated, and purified by preparative HPLC (Luna Axia C18 30×100 mm, 5 μm), eluting with MeCN-water containing 0.1% TFA (gradient from 30-90%, 30 mL/min) to give 4-(3-(5-chloro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (mixture of four diastereomers) as a yellow solid (14 mg, 39% yield). Mass spectrum 553 (M+H)+. 1H NMR (400 MHz, chloroform-d) δ 10.54 (d, J=5.9 Hz, 1H), 8.30 (ddt, J=19.6, 7.6, 0.9 Hz, 1H), 7.70-7.62 (m, 2H), 7.54-7.49 (m, 1H), 7.45-7.41 (m, 1H), 7.37-7.33 (m, 1H), 7.26-7.20 (m, 2H), 7.13 (dd, J=12.1, 7.7 Hz, 1H), 6.37 (td, J=7.3, 5.1 Hz, 1H), 6.32 (d, J=3.1 Hz, 1H), 1.89 (s, 3H), 1.65-1.63 (m, 6H).
Examples 14 and 15
4-(3-(R)-(5-Chloro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (14), and
4-(3-(S)-(5-Chloro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (15)




A sample of 4-(3-(5-chloro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (mixture of four diastereomers) [Example 13] was separated by chiral super-critical fluid chromatography as follows: First pass: column: Lux Cellulose-3 (3×25 cm, 5 μm); Mobile Phase: CO2-MeOH (70:30) at 140 mL/min, 100 bar, 35° C.; sample preparation: 13 mg/mL in MeOH; injection: 4.5 mL; Second pass: column: CHIRALCEL® AS (2×50 cm, 10 μm); Mobile Phase: CO2-MeOH (55:45) at 120 mL/min, 100 bar, 35° C.
The first and third peaks eluting from the column were combined to provide a mixture of two interconverting diastereomers of 4-(3-(R)-(5-chloro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide. [Example 14]. Mass spectrum m/z 553 (M+H)+. 1H NMR (400 MHz, DMSO-d6) δ 11.39 (d, J=3.7 Hz, 1H), 8.34-8.21 (m, 1H), 8.16 (br. s., 1H), 7.99 (dd, J=7.7, 1.5 Hz, 1H), 7.83 (s, 1H), 7.60 (d, J=7.0 Hz, 1H), 7.53-7.41 (m, 3H), 7.35 (d, J=7.3 Hz, 1H), 7.09-6.94 (m, 3H), 6.57 (td, J=7.3, 3.6 Hz, 1H), 6.00 (d, J=18.9 Hz, 1H), 4.97 (s, 1H), 1.75 (m, 3H), 1.46 (m, 6H).
The second and fourth peaks eluting from the column were combined to provide a mixture of two other interconverting diastereomers of 4-(3-(S)-(5-chloro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide. [Example 15]. Mass spectrum m/z 553 (M+H)+. 1H NMR (400 MHz, DMSO-d6) δ 11.39 (d, J=4.0 Hz, 1H), 8.36-8.21 (m, 1H), 8.16 (br. s., 1H), 7.99 (dd, J=7.7, 1.8 Hz, 1H), 7.83 (s, 1H), 7.60 (d, J=7.0 Hz, 1H), 7.54-7.41 (m, 3H), 7.35 (d, J=7.5 Hz, 1H), 7.08-6.94 (m, 3H), 6.57 (td, J=7.3, 3.6 Hz, 1H), 6.00 (d, J=18.7 Hz, 1H), 4.97 (d, J=0.9 Hz, 1H), 1.75 (m, 3H), 1.46 (m, 6H).
Alternative Synthesis of Example 15
4-(3-(S)-(5-Chloro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (Mixture of Two Interconverting diastereomers)




A solution of 4-bromo-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide [synthesized according to the procedure described in U.S. Pat. No. 8,084,620, Intermediate 73-2] (0.16 g, 0.461 mmol), 5-chloro-2-(S)-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione (single enantiomer) [Intermediate 15] (0.209 g, 0.507 mmol) and 3 M aqueous K3PO4 (0.384 mL, 1.15 mmol) in THF (3.0 mL) was bubbled with argon for 5 min. The mixture was treated with 1,1′-bis(di-t-butylphosphino)ferrocene palladium dichloride (15 mg, 23 μmol) and bubbling was continued for 30 sec. The reaction vessel was sealed, and subjected to three cycles of evacuation and filling with argon. The mixture was stirred overnight at room temperature, then was diluted with EtOAc, washed sequentially with water and brine, and the combined aqueous layers were extracted with EtOAc. The combined organic phases were dried and concentrated. The residue was combined with that from an identical reaction done on one-quarter of the scale and purified by chromatography to give 4-(3-(S)-(5-chloro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (mixture of two interconverting diastereomers) as a yellow solid (154 mg, 48% yield).
Example 16
4-(3-(5-Chloro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-pivalamido-9H-carbazole-1-carboxamide (Mixture of Four diastereomers)




A mixture of 4-bromo-7-pivalamido-9H-carbazole-1-carboxamide [synthesized according to the procedure described in U.S. Pat. No. 8,084,620, Example 57-51] (0.062 g, 0.160 mmol), 5-chloro-2-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione [Intermediate 13] (0.055 g, 0.133 mmol) and Cs2CO3 (0.087 g, 0.267 mmol) in THF (2.5 mL) and water (0.625 mL) was bubbled with nitrogen for 2 min, then was treated with PdCl2(dppf) DCM adduct (5.4 mg, 6.66 μmol). Bubbling was continued for 30 sec, then the mixture was heated at 60° C. for 5 h and stirred overnight at room temperature. The mixture was diluted with EtOAc, dried and concentrated. The residue was purified by column chromatography on silica gel, eluting with EtOAc-hexanes, to give 4-(3-(5-chloro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-pivalamido-9H-carbazole-1-carboxamide (mixture of four diastereomers) as a yellow solid (38 mg, 42% yield). Mass spectrum m/z 594 (M+H)+. 1H NMR (400 MHz, DMSO-d6) δ 11.42 (d, J=9.0 Hz, 1H), 9.23 (s, 1H), 8.29-8.22 (m, 1H), 8.15 (br. s., 1H), 8.09 (dd, J=15.5, 1.7 Hz, 1H), 7.96 (dd, J=7.8, 2.1 Hz, 1H), 7.62-7.58 (m, 1H), 7.54-7.36 (m, 4H), 7.02 (s, 3H), 6.57 (dt, J=9.3, 7.3 Hz, 1H), 5.99 (d, J=6.2 Hz, 1H), 1.73 (d, J=3.1 Hz, 3H), 1.24 (d, J=1.1 Hz, 9H).
Example 17
4-(3-(5-Chloro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(methoxymethyl)-9H-carbazole-1-carboxamide (Mixture of Four diastereomers)




A mixture of 4-bromo-7-(methoxymethyl)-9H-carbazole-1-carboxamide [Intermediate 25] (31.1 mg, 0.093 mmol), 5-chloro-2-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione [Intermediate 13] (35 mg, 0.085 mmol) and Cs2CO3 (55.3 mg, 0.170 mmol) in THF (1.5 mL) and water (0.375 mL) was bubbled with argon for 3 min. The mixture was treated with PdCl2(dppf) DCM adduct (3.5 mg, 4.24 μmol) and bubbling was continued for 1 min. The mixture was heated at 45° C. for 5 h and cooled to room temperature. The mixture was partitioned between EtOAc and water, the organic layer was washed with brine and the combined aqueous layers were extracted with EtOAc. The combined organic phases were dried and concentrated, and the residue was purified by preparative HPLC. Product-containing fractions were treated with saturated aqueous NaHCO3, combined and extracted with EtOAc. The organic layer was dried and concentrated, and the residue was purified twice by column chromatography on silica gel (24 g, then 12 g), eluting with EtOAc, to provide 4-(3-(5-chloro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(methoxymethyl)-9H-carbazole-1-carboxamide (mixture of four diastereomers) as a yellow solid (9.7 mg, 20% yield). Mass spectrum m/z 539 (M+H)+. 1H NMR (400 MHz, DMSO-d6) δ 11.53-11.51 (m, 1H), 8.33-8.28 (m, 1H), 8.19 (br. s., 1H), 8.05-8.00 (m, 1H), 7.67 (s, 1H), 7.60 (d, J=6.6 Hz, 1H), 7.54-7.42 (m, 5H), 7.38 (dd, J=7.5, 1.3 Hz, 1H), 7.07-7.00 (m, 2H), 6.85 (dd, J=8.3, 1.4 Hz, 1H), 6.61-6.53 (m, 2H), 5.97 (s, 1H), 4.49 (s, 2H), 3.29 (s, 3H).
Example 18
4-(3-(5-Fluoro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(methoxymethyl)-9H-carbazole-1-carboxamide (Mixture of Four diastereomers)




A mixture of 4-bromo-7-(methoxymethyl)-9H-carbazole-1-carboxamide [Intermediate 25] (0.05 g, 0.150 mmol), 5-fluoro-2-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione [Intermediate 18] (0.065 g, 0.165 mmol) and Cs2CO3 (0.098 g, 0.300 mmol) in dioxane (2.0 mL) and water (0.5 mL) was bubbled with argon for 2 min. The mixture was treated with PdCl2(dppf) DCM adduct (6.1 mg, 7.50 μmol), bubbling with argon for 30 sec more, then heated for 6 h at 50° C. The cooled mixture was diluted with DCM-MeOH, dried and concentrated. The residue was purified by column chromatography on silica gel (40 g), eluting with EtOAc-hexanes (gradient from 80-100%), to give 4-(3-(5-fluoro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(methoxymethyl)-9H-carbazole-1-carboxamide (mixture of four diastereomers) as a yellow solid (0.0481 g, 57% yield). Mass spectrum m/z 523 (M+H)+. 1H NMR (400 MHz, DMSO-d6) δ 11.51 (d, J=5.7 Hz, 1H), 8.19 (br. s., 1H), 8.16-8.06 (m, 2H), 8.02 (dd, J=7.8, 1.9 Hz, 1H), 7.67 (d, J=3.5 Hz, 1H), 7.55-7.42 (m, 5H), 7.38 (d, J=7.5 Hz, 1H), 7.23 (t, J=8.7 Hz, 1H), 7.05 (d, J=7.7 Hz, 1H), 6.85 (ddd, J=8.2, 3.8, 1.4 Hz, 1H), 6.60-6.52 (m, 1H), 5.86 (d, J=15.0 Hz, 2H), 4.49 (s, 2H), 3.29 (d, J=1.1 Hz, 3H).
Example 19
4-(3-(4-Fluoro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (Mixture of Four diastereomers)




A mixture of 4-bromo-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide [synthesized according to the procedure described in U.S. Pat. No. 8,084,620, Intermediate 73-2] (0.034 g, 0.097 mmol), 4-fluoro-2-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione [Intermediate 16] (0.035 g, 0.088 mmol) and Cs2CO3 (0.058 g, 0.177 mmol) in THF (1.5 mL) and water (0.375 mL) was bubbled with argon for 3 min. The mixture was treated with PdCl2(dppf) DCM adduct (3.6 mg, 4.42 μmol), bubbled with argon for 1 min more, and heated at 45° C. After 5 h, the cooled mixture was diluted with EtOAc and washed sequentially with water and brine. The combined aqueous layers were extracted with EtOAc, and the combined organic phases were dried and concentrated. The residue was purified by preparative HPLC. Fractions containing the product were treated with saturated aqueous NaHCO3, combined and partitioned between EtOAc and saturated aqueous NaHCO3. The organic layer was washed with brine, dried and concentrated to give 4-(3-(4-fluoro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (mixture of four diastereomers) as a yellow solid (0.0109 g, 22% yield). Mass spectrum m/z 537 (M+H)+. 1H NMR (400 MHz, DMSO-d6) δ 11.39 (d, J=2.4 Hz, 1H), 8.27-8.13 (m, 2H), 7.99 (dd, J=7.8, 2.3 Hz, 2H), 7.83 (s, 1H), 7.55-7.43 (m, 3H), 7.40-7.26 (m, 2H), 7.07-6.94 (m, 3H), 6.64-6.55 (m, 1H), 4.97 (d, J=4.8 Hz, 1H), 1.75 (d, J=1.3 Hz, 3H), 1.48-1.43 (m, 6H).
Example 20
4-(3-(5,7-Dioxo-5H-thiazolo[3,2-c]pyrimidin-6(7H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (Mixture of Four diastereomers)




A mixture of 4-bromo-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide [synthesized according to the procedure described in U.S. Pat. No. 8,084,620, Intermediate 73-2] (0.040 g, 0.115 mmol), 6-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-5H-thiazolo[3,2-c]pyrimidine-5,7(6H)-dione [Intermediate 17] (0.04 g, 0.104 mmol) and Cs2CO3 (0.068 g, 0.208 mmol) in THF (2.0 mL) and water (0.5 mL) was bubbled with argon for 3 min, then was treated with PdCl2(dppf) DCM adduct (4.3 mg, 5.20 μmol). Bubbling was continued for 30 sec more, then the mixture heated at 50° C. for 5 h. The cooled mixture was partitioned between EtOAc and water, and the organic layer was washed with brine. The combined aqueous layers were extracted with EtOAc, and the combined organic phases were dried and concentrated. The residue was purified by column chromatography on silica gel (40 g), eluting with EtOAc, to give 4-(3-(5,7-dioxo-5H-thiazolo[3,2-c]pyrimidin-6(7H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (mixture of four diastereomers) as a reddish solid (0.032 g, 56% yield). Mass spectrum m/z 525 (M+H)+. 1H NMR (400 MHz, DMSO-d6) δ 11.38 (d, J=4.8 Hz, 1H), 8.16 (br. s., 1H), 7.98 (dd, J=7.8, 1.2 Hz, 1H), 7.83 (d, J=1.8 Hz, 1H), 7.75-7.64 (m, 1H), 7.51-7.39 (m, 3H), 7.33 (dd, J=7.4, 1.2 Hz, 1H), 7.07-6.92 (m, 4H), 6.29 (d, J=13.6 Hz, 1H), 4.97 (s, 1H), 1.75-1.73 (m, 3H), 1.47-1.43 (m, 6H).
Example 21
4-(3-(5-Fluoro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (Mixture of Four diastereomers)




A mixture of 4-bromo-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide [synthesized according to the procedure described in U.S. Pat. No. 8,084,620, Intermediate 73-2] (0.048 g, 0.139 mmol), 5-fluoro-2-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione [Intermediate 18] (0.050 g, 0.126 mmol) and Cs2CO3 (0.082 g, 0.252 mmol) in THF (2.0 mL) and water (0.5 mL) was bubbled with nitrogen for 2 min. The mixture was treated with PdCl2(dppf) DCM adduct (5.2 mg, 6.31 μmol), bubbling was continued for 30 sec and the vial was sealed. The mixture was heated at 50° C. for 5 h. The cooled mixture was diluted with EtOAc and washed sequentially with water and brine. The combined aqueous layers were extracted with DCM. The combined organic layers were dried and concentrated. The residue was purified by column chromatography on silica gel to provide 4-(3-(5-fluoro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (mixture of four diastereomers) as a yellow solid (0.0375 g, 54% yield). Mass spectrum m/z 537 (M+H)+. 1H NMR (400 MHz, DMSO-d6) δ 11.38 (d, J=4.6 Hz, 1H), 8.21-8.03 (m, 2H), 7.98 (dd, J=7.9, 1.5 Hz, 1H), 7.86-7.78 (m, 1H), 7.54-7.39 (m, 3H), 7.35 (d, J=7.5 Hz, 1H), 7.23 (t, J=8.9 Hz, 1H), 7.09-6.92 (m, 3H), 6.61-6.50 (m, 1H), 5.86 (d, J=19.4 Hz, 1H), 4.96 (d, J=1.8 Hz, 1H), 1.74 (d, J=2.6 Hz, 3H), 1.45 (d, J=4.6 Hz, 6H).
Examples 22 and 23
4-(3-(5-Fluoro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (Mixtures of Two Interconverting diastereomers)




A sample of 4-(3-(5-fluoro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (mixture of four diastereomers) [Example 21] was separated by chiral super-critical fluid chromatography as follows: First pass: column: CHIRALPAK® IA (3×25 cm, 5 μm); Mobile Phase: CO2-MeOH (50:50) at 150 mL/min, 100 bar, 40° C.; sample preparation: 7 mg/mL in MeOH-DCM (1:1) with added DMSO; injection: 2 mL; Second pass: column: CHIRALCEL® OD-H (3×25 cm, 5 μm); Mobile Phase: CO2-MeOH (50:50) at 120 mL/min, 100 bar, 35° C.; sample preparation: 3.7 mg/mL in MeOH-chloroform (3:1); injection: 4 mL.
The first and second peaks eluting from the column were combined to provide a mixture of two interconverting diastereomers of 4-(3-(5-fluoro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide. [Example 22]. Mass spectrum m/z 519 (M+H−H2O)+. 1H NMR (400 MHz, DMSO-d6) δ 11.42-11.36 (m, 1H), 8.20-8.06 (m, 2H), 7.99 (d, J=7.0 Hz, 1H), 7.83 (br. s., 1H), 7.54-7.41 (m, 3H), 7.35 (d, J=7.3 Hz, 1H), 7.23 (t, J=8.7 Hz, 1H), 7.09-6.94 (m, 3H), 6.61-6.51 (m, 1H), 5.87 (d, J=19.6 Hz, 1H), 4.97 (s, 1H), 1.75 (s, 3H), 1.48-1.42 (m, 6H).
The third and fourth peaks eluting from the column were combined to provide another mixture of two interconverting diastereomers of 4-(3-(5-fluoro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide as a yellow solid. [Example 23]. Mass spectrum m/z 519 (M+H−H2O)+. 1H NMR (400 MHz, DMSO-d6) δ 11.41-11.37 (m, 1H), 8.21-8.06 (m, 2H), 8.02-7.96 (m, 1H), 7.83 (br. s., 1H), 7.54-7.41 (m, 3H), 7.35 (d, J=7.3 Hz, 1H), 7.23 (t, J=8.6 Hz, 1H), 7.08-6.95 (m, 3H), 6.60-6.52 (m, 1H), 5.87 (d, J=19.6 Hz, 1H), 4.99-4.95 (m, 1H), 1.78-1.73 (m, 3H), 1.48-1.43 (m, 6H).
Alternative Synthesis of Example 22:
Using the procedure of the Alternative Synthesis of Example 15, 4-bromo-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide [synthesized according to the procedure described in U.S. Pat. No. 8,084,620, Intermediate 73-2] and 5-fluoro-2-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione (single enantiomer) [Intermediate 19] were converted to 4-(3-(5-fluoro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (mixture of two interconverting diastereomers) as a yellow solid.
Alternative Synthesis of Example 23:
Using the procedure of the Alternative Synthesis of Example 15, 4-bromo-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide [synthesized according to the procedure described in U.S. Pat. No. 8,084,620, Intermediate 73-2] and 5-fluoro-2-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-1H-pyrido[1,2-c]pyrimidine-1,3(2H)-dione (single enantiomer) [Intermediate 20] were converted to 4-(3-(5-fluoro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (mixture of two interconverting diastereomers) as a yellow solid.
Example 24
4-(3-(S)-(8-Fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-5-methyl-9H-carbazole-1-carboxamide (Mixture of Two diastereomers)




A mixture of 4-bromo-7-(2-hydroxypropan-2-yl)-5-methyl-9H-carbazole-1-carboxamide [Intermediate 22] (45 mg, 0.125 mmol), 8-fluoro-1-methyl-3-(S)-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)quinazoline-2,4(1H,3H)-dione [Intermediate 3] (61 mg, 0.149 mmol), and 2.0 M aqueous K3PO4 (0.187 mL, 0.374 mmol) in THF (1.25 mL) in a vial was bubbled with argon for 1 min while swirling in an ultrasonic water bath. The mixture was treated with 1,1′-bis(di-t-butylphosphino)ferrocene palladium(II) chloride (5.1 mg, 6.23 μmol) and the vial was sealed and heated at 45° C. After 15.25 h the mixture was cooled to room temperature and concentrated. The residue was sonicated in acetonitrile and the supernatant was filtered and purified by preparative HPLC (PHENOMENEX® Axia C18), eluting with MeCN-water containing 0.1% TFA (gradient from 10-100%). The fractions containing the product were treated with saturated aqueous NaHCO3 and concentrated to provide an aqueous suspension. The precipitate was collected by filtration, washed with water and dried to provide 4-(3-(S)-(8-fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-5-methyl-9H-carbazole-1-carboxamide (mixture of two diastereomers) as a white solid (49.4 mg, 67% yield). Mass spectrum m/z 547 (M+H−H2O)+. 1H NMR (400 MHz, MeOH-d4) δ 8.05 (t, J=8.1 Hz, 1H), 7.91 (d, J=7.7 Hz, 1H), 7.61 (ddd, J=14.3, 8.1, 1.4 Hz, 1H), 7.57 (s, 1H), 7.54-7.48 (m, 1H), 7.47-7.42 (m, 1H), 7.37-7.28 (m, 2H), 7.02 (dd, J=7.9, 1.3 Hz, 2H), 3.85 (2d, J=3.7 Hz, 3H), 1.92 (2s, 3H), 1.76 (2s, 3H), 1.61 (s, 6H).
Example 25
4-(3-(S)-(8-Fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-5-methyl-9H-carbazole-1-carboxamide (Single diastereomer)




4-(3-(S)-(8-Fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-5-methyl-9H-carbazole-1-carboxamide (mixture of two diastereomers) [Example 24] (42.7 mg, 0.076 mmol) was resolved by chiral super-critical fluid chromatography as follows: column: Regis WHELK-O® R,R (3×25, 5 μm); Mobile Phase: CO2-MeOH (60:40) at 85 mL/min, 100 bar; sample preparation: 10.7 mg/mL in MeCN-MeOH (9:1); injection: 0.50 mL. The second peak eluting from the column was concentrated to give a single diastereomer @resumed to be 4-(R)-(3-(S)-(8-fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide) as an off-white solid (13.0 mg). The chiral purity was found to be 89%. Mass spectrum m/z 547 (M+H−H2O)+. 1H NMR (400 MHz, MeOH-d4) δ 8.04 (d, J=7.5 Hz, 1H), 7.90 (d, J=7.7 Hz, 1H), 7.65-7.55 (m, 2H), 7.52-7.48 (m, 1H), 7.47-7.41 (m, 1H), 7.35-7.27 (m, 2H), 7.05-6.97 (m, 2H), 3.85 (d, J=7.9 Hz, 3H), 1.91 (s, 3H), 1.75 (s, 3H), 1.61 (s, 6H).
Example 26
4-(3-(S)-(8-Fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-8-methyl-9H-carbazole-1-carboxamide (Mixture of Two Interconverting diastereomers)




A mixture of 4-bromo-7-(2-hydroxypropan-2-yl)-8-methyl-9H-carbazole-1-carboxamide [Intermediate 23] (30 mg, 0.083 mmol), 8-fluoro-1-methyl-3-(S)-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)quinazoline-2,4(1H,3H)-dione [Intermediate 3] (40.9 mg, 0.100 mmol), and 2.0 M aqueous K3PO4 (125 μL, 0.249 mmol) in THF (1 mL) in a vial was bubbled with argon for 1 min with sonication. The mixture was treated with 1,1′-bis(di-t-butylphosphino)ferrocene palladium(II) chloride (3.4 mg, 4.15 μmol) and the tube was sealed and heated at 45° C. After 15.25 h the mixture was cooled to room temperature and concentrated. The residue was sonicated in MeCN and the supernatant was filtered and purified by preparative HPLC (PHENOMENEX® Axia C18 30×100 mm), eluting with MeCN-water containing 0.1% TFA (gradient from 10-100%). The appropriate fractions were combined, treated with saturated aqueous NaHCO3 and concentrated to provide an aqueous suspension. The precipitate was collected by filtration, washed with water and dried under vacuum to provide 4-(3-(S)-(8-fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-8-methyl-9H-carbazole-1-carboxamide (mixture of two interconverting diastereomers) as an off-white solid (33 mg, 70% yield). Mass spectrum m/z 547 (M+H−H2O)+. 1H NMR (400 MHz, MeOH-d4) δ 8.08 (dd, J=17.3, 7.6 Hz, 1H), 7.98 (d, J=7.7 Hz, 1H), 7.62 (dd, J=14.1, 8.1 Hz, 1H), 7.56-7.50 (m, 1H), 7.46-7.38 (m, 2H), 7.38-7.30 (m, 1H), 7.23 (dd, J=12.5, 8.6 Hz, 1H), 7.11 (dd, J=7.8, 1.4 Hz, 1H), 7.05 (t, J=8.7 Hz, 1H), 3.88 (dd, J=10.5, 7.8 Hz, 3H), 2.85 (s, 3H), 1.83 (s, 3H), 1.71 (d, J=2.0 Hz, 6H).
Example 27
4-(3-(S)-(8-Fluoro-1-methyl(d3)-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-5-methyl-9H-carbazole-1-carboxamide (Mixture of Two diastereomers)




A mixture of 4-bromo-7-(2-hydroxypropan-2-yl)-5-methyl-9H-carbazole-1-carboxamide [Intermediate 22] (45 mg, 0.125 mmol), 8-fluoro-1-methyl(d3)-3-(S)-(2-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)quinazoline-2,4(1H,3H)-dione [Intermediate 24] (62 mg, 0.149 mmol), and 2.0 M aqueous K3PO4, (0.187 mL, 0.374 mmol) in THF (1.25 mL) in a vial was bubbled with argon for 1 min with sonication. The mixture was treated with 1,1′-bis(di-t-butylphosphino)ferrocene palladium(II) chloride (5.1 mg, 6.23 μmol) and the tube was sealed and heated at 45° C. After 16.5 h the mixture was cooled to room temperature and concentrated. The residue was sonicated in MeCN, filtered and purified by preparative HPLC (PHENOMENEX® Axia C18 30×100 mm), eluting with MeCN-water containing 0.1% TFA (gradient from 10-100%). The product-containing fractions were combined, treated with saturated aqueous NaHCO3 and concentrated to an aqueous suspension. The precipitate was collected by filtration, rinsed with water and dried under vacuum to provide 4-(3-(S)-(8-fluoro-1-methyl(d3)-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-5-methyl-9H-carbazole-1-carboxamide (mixture of two diastereomers) as an off-white solid (41.0 mg, 57% yield). Mass spectrum m/z 550 (M+H−H2O)+. 1H NMR (400 MHz, MeOH-d4) δ 8.05 (t, J=8.1 Hz, 1H), 7.91 (d, J=7.7 Hz, 1H), 7.61 (ddd, J=14.3, 8.1, 1.4 Hz, 1H), 7.57 (s, 1H), 7.54-7.48 (m, 1H), 7.47-7.42 (m, 1H), 7.37-7.28 (m, 2H), 7.02 (dd, J=7.9, 1.3 Hz, 2H), 1.92 (2s, 3H), 1.76 (2s, 3H), 1.61 (s, 6H)
The compounds in Table 2 were prepared by procedures analogous to those described above, using Intermediates described or prepared by methods similar to those described or by methods available to those skilled in the art.










TABLE 2








Obs. m/z


Ex
Structure
FW
(M + H)+


















28




508.51
509


 


29




535.58
536


 


30




549.56
550


 


31




578.07
579


 


32




522.53
523


 


33




568.58
569.3









Comparative Example 34
7-(2-Hydroxypropan-2-yl)-4-(2-methyl-3-(4-oxoquinazolin-3(4H)-yl)phenyl)-9H-carbazole-1-carboxamide




Comparative Example 34 was disclosed in U.S. Pat. No. 8,084,620 as Example 76-15 and was prepared according to the procedure described therein.
Comparative Example 35
7-(2-Hydroxypropan-2-yl)-3-methyl-4-(2-methyl-3-(4-oxoquinazolin-3(4H)-yl)phenyl)-9H-pyrido[3,4-b]indole-1-carboxamide




Comparative Example 35 was disclosed in WO 2011/159857 as Example 38 and was prepared according to the procedure described therein.
BIOLOGICAL ASSAYS
Human Recombinant Btk Enzyme Assay
To V-bottom 384-well plates were added test compounds, human recombinant Btk (1 nM, INVITROGEN® Corporation), fluoresceinated peptide (1.5 μM), ATP (20 μM), and assay buffer (20 mM HEPES pH 7.4, 10 mM MgCl2, 0.015% Brij 35 surfactant and 4 mM DTT in 1.6% DMSO), with a final volume of 30 μL. After incubating at room temperature for 60 min, the reaction was terminated by adding 45 μL of 35 mM EDTA to each sample. The reaction mixture was analyzed on the Caliper LABCHIP® 3000 (Caliper, Hopkinton, Mass.) by electrophoretic separation of the fluorescent substrate and phosphorylated product. Inhibition data were calculated by comparison to no enzyme control reactions for 100% inhibition and no inhibitor controls for 0% inhibition. Dose response curves were generated to determine the concentration required for inhibiting 50% of kinase activity (IC50). Compounds were dissolved at 10 mM in DMSO and evaluated at eleven concentrations.
Ramos FLIPR Assay
Ramos RA1 B cells (ATCC CRL-1596) at a density of 2×106 cells/mL in RPMI minus phenol red (Invitrogen 11835-030) and 50 mM HEPES (Invitrogen 15630-130) containing 0.1% BSA (Sigma A8577) were added to one half volume of calcium loading buffer (BD bulk kit for probenecid sensitive assays, #640177) and incubated at room temperature in the dark for 1 hour. Dye-loaded cells were pelleted (Beckmann GS-CKR, 1200 rpm, room temperature, 5 min) and resuspended at room temperature in RPMI minus phenol red with 50 mM HEPES and 10% FBS to a density of 1×106 cells/mL. 150 μL aliquots (150,000/well) were plated into 96 well poly-D-lysine coated assay plates (BD 35 4640) and briefly centrifuged (Beckmann GS-CKR 800 rpm, 5 min, without brake). Next, 50 μL compound dilutions in 0.4% DMSO/RPMI minus phenol red+50 mM HEPES+10% FBS were added to the wells and the plate was incubated at room temperature in the dark for 1 hour. The assay plate was briefly centrifuged as above prior to measuring calcium levels.
Using the FLIPR1 (Molecular Devices), cells were stimulated by adding goat anti-human IgM (Invitrogen AHI0601) to 2.5 μg/mL. Changes in intracellular calcium concentrations were measured for 180 seconds and percent inhibition was determined relative to peak calcium levels seen in the presence of stimulation only.
Jak2 Tyrosine Kinase Assay
Compounds with activity against Jak2 tyrosine kinase have been observed to cause thrombocytopenia, anemia and neutropenia in human patients in clinical trials (see for example, see Pardanani, A., Leukemia, 26:1449 (2012)). Jak2 signaling occurs thru EPO and TPO, which control erythrocyte and platelet proliferation, respectively. Thus, inhibition of Jak2 tyrosine kinase can potentially lead to side-effects in the clinic. Btk inhibitors with improved selectivity over Jak2 tyrosine kinase are desired in order to minimize off target side-effects related to the inhibition of Jak2 tyrosine kinase.
The assays were performed in V-bottom 384-well plates. The final assay volume was 30 μl prepared from 15 μl additions of enzyme and substrates (fluoresceinated peptide and ATP) and test compounds in assay buffer (100 mM HEPES pH 7.4, 10 mM MgCl2, 25 mM beta-glycerolphosphate, 0.015% Brij 35 surfactant and 4 mM DTT). The reaction was initiated by the combination of Jak2 tyrosine kinase with substrates and test compounds. The reaction mixture was incubated at room temperature for 60 minutes and terminated by adding 45 μL of 35 mM EDTA to each sample. The reaction mixture was analyzed on the Caliper LABCHIP® 3000 by electrophoretic separation of the fluorescent substrate and phosphorylated product. Inhibition data were calculated by comparison to no enzyme control reactions for 100% inhibition and vehicle-only reactions for 0% inhibition. The final concentration of reagents in the assays is ATP, 30 μM; Jak2 fluorescent peptide, 1.5 μM; Jak2, 1 nM; and DMSO, 1.6%. Dose response curves were generated to determine the concentration required inhibiting 50% of kinase activity (IC50). Compounds were dissolved at 10 mM in DMSO and evaluated at eleven concentrations, each in duplicate. IC50 values were derived by non-linear regression analysis.
Whole Blood Assays of BCR-Stimulated CD69 Expression on B Cells
The efficacy of Btk inhibitor compounds in suppressing CD69 expression on B cells human in whole blood assays is useful for predicting efficacious doses in the clinic and minimizing potential side-effects. Btk inhibitor compounds having higher activity in the whole blood CD69 expression assay are expected to require lower doses than compounds having lower activity, and are expected to cause fewer unwanted side-effects. (Uetrecht, Chem. Res. Toxicol., 12, 387-395 (1999); Nakayama, Drug Metabolism and Disposition, 37(9):1970-1977 (2009); Sakatis, Chem. Res. Toxicol. (2012)).
To measure BCR-stimulated B cells, ACD-A human whole blood was treated with various concentrations of test compound and stimulated with 30 μg/mL AffiniPure F(ab′)2 fragment goat anti human IgM (Jackson 109-006-1299—endotoxin cleared) and 10 ng/mL human IL-4 (Peprotech 200-04) for 18 h at 37° C. with agitation. The cells were blocked with human gamma globulin (Jackson 009-000-002) and stained with FITC-conjugated mouse anti-human CD20 (BD Pharmingen 555622) and PE-conjugated mouse anti-human CD69 monoclonal antibody (BD Pharmingen 555531), lysed and fixed, then washed. The amount of CD69 expression was quantitated by the median fluorescence intensity (MFI) after gating on the CD20-positive B cell population as measured by FACS analysis.
In the whole blood assay of BCR-Stimulated CD69 expression on B cells, increased efficacy of a Btk inhibitor compound is indicated by a lower CD69 IC50 value.











TABLE 3








Ratio of 
CD69 IC50 



Btk IC50 
Jak2 IC50 
Jak2/Btk
values


Example
value (nM)
value (nM)
IC50 values
(nM)



















 1
1.5
1200
800
140


 2
2.4
850
350
200


 3
2.3
1200
520
190


 4
4.4
2000
450
190


 5
0.9
1200
1300
180


 6
5.3
1800
340
150


 7
1.7
1300
760
220


 8
5.8
2200
380
160


 9
2.2
1000
450
210


10
1.4
1100
790
120


11
0.63
1000
1600
140


12
0.52
980
1900
140


13
0.94
1300
1400
120


14
1.0
1100
1100
78


15
0.41
1000
2400
59


16
2.1
2200
1000
220


17
1.7
1900
1100
110


18
2.3
740
320
130


19
1.6
1900
1200
140


20
1.6
570
360
130


21
0.93
960
1000
75


22
1.7
880
520
91


23
1.2
1000
830
79


24
0.59
>2000
>3400
160


25
0.70
4600
6600
69


26
0.84
>2000
>2400
200


27
1.2
>2000
>1700
98


28
1.5
800
530
87


29
1.5
470
310
56


30
1.2
430
360
130


31
0.54
79
150
60


32
2.3
740
320
130


33
0.4
226
540
250


Comparative
2.6
240
92
650


Ex. 34






Comparative
6.9
200
29
—


Ex. 35













The compounds of the present invention, as exemplified by Examples 1 to 33, have been compared to Comparative Examples 34 and 35, disclosed in U.S. Pat. No. 8,084,620 and WO 2011/159857, respectively, and have been found to be advantageous. The compounds of the present invention have the surprising advantage of the combination of Btk inhibition activity and improved kinase selectivity of Btk inhibition activity over Jak2 inhibition activity. As shown in Table 3, in the reported tests, Examples 1 to 33 show the surprising advantage of the combination of efficacy of Btk inhibition activity and improved kinase selectivity of Btk inhibition activity over Jak2 inhibition activity, as characterized by the ratio of Jak2/Btk IC50 values. Increased selectivity for Btk kinase over Jak2 kinase is indicated by a larger value for the ratio of the Jak2/Btk IC50 values. Examples 1 to 33 had Btk IC50 values of less than 6 nM and ratios of Jak2/Btk IC50 values of 150 and greater. In contrast, Comparative Examples 34 and 35 had Btk IC50 values of 2.6 and 6.9 nM and ratios of Jak2/Btk IC50 values of 92 and 29, respectively.
Additionally, the compounds of the present invention, as exemplified by Examples 1 to 33, also have improved potency in the whole blood BCR-stimulated CD69 expression assay, compared to Comparative Example 34. As shown in Table 3, in the reported tests, Examples 1 to 33 show the surprising advantage of the combination of efficacy of Btk inhibition activity, improved kinase selectivity of Btk inhibition activity over Jak2 inhibition activity, and improved potency in the whole blood BCR-stimulated CD69 expression assay. Examples 1 to 33 had Btk IC50 values of less than 6 nM, ratios of Jak2/Btk IC50 values of 150 and greater, and CD69 IC50 values of 250 nM and less. In contrast, Comparative Example 34 had a Btk IC50 value of 2.6 nM, a ratio of Jak2/Btk IC50 value of 92, and a CD69 IC50 values of 650 nM.







TABLE 4







Unit Cell Parameters of Crystal Forms of Example 3 Determined by Single Crystal


X-Ray Diffractometry


























Space
Molecules/
Density, calc.



Form
a (Å)
b (Å)
c (Å)
α (°)
β (°)
γ (°)
Volume
Group
asymmetric unit (Z′)
(g/cm3)
Temp





















SA-1
10.9201
11.5031
23.7434
90
99.2590
90
2943.7
P21
2
1.355
RT


SB-2
11.5337
11.7474
23.2486
90
90.6120
90
3149.8
P21
2
1.340
203 K


SE-2
11.1799
11.9237
22.9237
90
91.2000
90
3059.2
P21
2
1.315
203 K


SC-3
14.4552
11.8207
18.8382
90
93.829
90
3211.7
P21
2
1.296
203 K


SD-3
14.5877
11.8964
19.2500
90
91.506
90
3339.5
P21
2
1.346
203 K


M2-4
11.1815
17.3429
31.6262
90
90
90
6132.9
P212121
2
1.331
203 K


AN-5
12.9874
11.4967
20.3900
90
105.829
90
2929.0
P21
2
1.342
203 K


H1-6
10.5755
11.5986
22.3697
90
90
90
2743.9
P212121
1
1.376
RT


E-7
9.5968
14.2611
21.6404
90
90
90
2961.7
P212121
1
1.338
RT


SE-8
11.4019
11.4019
45.6578
90
90
90
5935.7
P41212
1
1.376
203 K





RT = room temperature













TABLE 5







Fractional Atomic Coordinates for the SA-1 Form of Example 3


at Room Temperature














Atom
X
Y
Z
Atom
X
Y
Z

















O1
0.8236
0.1800
0.1919
H29
−0.0892
0.5178
−0.0396


O2
0.5888
0.2021
0.2235
C30
0.4451
0.4402
−0.3342


O3
0.0324
0.6009
−0.1143
C31
0.1424
0.7099
−0.0355


O4
0.3464
0.2872
−0.1889
C32
−0.0483
0.5902
−0.0287


O5
0.6503
0.0186
−0.1841
O6
0.1448
0.2717
0.6878


N1
0.3384
0.3498
0.0906
O7
−0.1602
0.5390
0.6823


N2
0.4907
0.1462
−0.1900
O8
0.4711
−0.0301
0.6158


N3
0.3883
0.2541
0.2099
O9
−0.0937
0.3580
0.2766


N4
0.6273
0.1447
−0.2577
N5
0.1593
0.2113
0.4097


F1
0.6959
0.2533
−0.3567
N6
0.0028
0.4150
0.6901


C1
0.5683
0.2426
−0.2839
N7
0.1054
0.3034
0.2900


C2
0.2055
0.4787
0.0211
N8
0.0263
0.2618
0.7584


C3
0.2782
0.3807
0.0370
C33
−0.1302
0.4102
0.7610


C4
0.1522
0.4914
−0.0355
C34
0.2952
0.0867
0.4801


C5
0.4001
0.2459
0.0866
C35
0.1443
0.5924
0.5604


C6
0.3005
0.2956
−0.0029
C36
0.0955
0.3131
0.4131


C7
0.3472
−0.0311
−0.0613
C37
0.0210
0.4335
0.5882


C8
0.4725
0.2953
−0.2608
C38
0.1129
0.3509
0.4712


C9
0.3804
0.2097
0.0294
C39
0.0733
0.4932
0.5467


C10
0.2458
0.3101
−0.0597
C40
0.2509
0.2545
0.5596


C11
0.6007
0.2942
−0.3326
C41
0.0505
0.4740
0.6440


C12
0.4714
0.1258
−0.0888
C42
0.1953
0.2667
0.5030


C13
0.5939
0.0977
−0.2090
C43
0.2194
0.1812
0.4637


C14
0.4200
0.0666
−0.0460
C44
0.0544
0.4511
0.4859


C15
0.4444
0.0844
−0.1444
C45
0.3499
0.0750
0.5368


C16
0.4878
0.2148
0.1912
C46
−0.1007
0.4603
0.7087


C17
0.5309
0.0834
0.1128
C47
−0.0217
0.5117
0.4432


C18
0.5149
0.0478
0.0566
C48
0.1692
0.6319
0.6159


C19
0.7264
0.0827
−0.2808
C49
0.0218
0.3764
0.3704


C20
0.4391
0.1079
0.0138
C50
0.0065
0.3453
0.3086


C21
0.3233
−0.0708
−0.1167
C51
0.3261
0.1601
0.5753


C22
0.1725
0.4055
−0.0755
C52
−0.0378
0.4751
0.3865


C23
0.4737
0.1818
0.1303
C53
0.3755
−0.1406
0.5350


C24
0.5401
0.3901
−0.3569
C54
0.1236
0.5709
0.6583


C25
0.4288
0.2466
−0.2113
C55
−0.0619
0.3305
0.5743


C26
0.0708
0.5973
−0.0536
C56
0.4363
−0.0274
0.5548


C27
0.3714
−0.0122
−0.1586
C57
0.0635
0.3127
0.7111


C28
0.5534
0.2298
−0.0749
C58
−0.0637
0.3141
0.7856


C29
0.4119
0.3943
−0.2853
C59
−0.1804
0.3287
0.8630


C61
0.5601
−0.0082
0.5338
C60
0.0852
0.1502
0.7767


C62
−0.0939
0.2755
0.8375
H30
0.4092
−0.0406
0.6307


C63
−0.2462
0.4231
0.8384
H31
0.1615
0.1723
0.3790


C64
−0.2213
0.4645
0.7866
H32
0.1011
0.2842
0.2547


F2
−0.0324
0.1855
0.8656
H33
0.1740
0.2955
0.3131


O10
0.1182
0.2705
0.1683
H34
0.3092
0.0313
0.4533


C65
0.0654
0.3781
0.1484
H35
0.1753
0.6326
0.5318


O11
−0.3278
0.3788
0.3082
H36
0.2375
0.3097
0.5865


O12
0.3739
0.3014
0.3316
H37
−0.0629
0.5779
0.4527


C66
0.4261
0.1981
0.3502
H38
0.2162
0.6987
0.6247


H1
0.8619
0.2308
0.2148
H39
0.3626
0.1527
0.6133


H2
0.7472
0.1765
0.1967
H40
−0.0892
0.5174
0.3589


H3
0.0906
0.6220
−0.1297
H41
0.4328
−0.2032
0.5455


H4
0.3374
0.3887
0.1214
H42
0.3516
−0.1390
0.4944


H5
0.3920
0.2718
0.2454
H43
0.3032
−0.1519
0.5527


H6
0.3196
0.2621
0.1867
H44
0.1419
0.5950
0.6960


H7
0.1928
0.5343
0.0481
H45
−0.0631
0.3087
0.5352


H8
0.3136
−0.0708
−0.0333
H46
−0.1445
0.3499
0.5801


H9
0.2588
0.2552
−0.0870
H47
−0.0314
0.2667
0.5987


H10
0.5821
0.0399
0.1400
H48
−0.1960
0.3012
0.8980


H11
0.5562
−0.0184
0.0471
H49
0.1472
0.1622
0.8098


H12
0.6961
0.0583
−0.3191
H50
0.1235
0.1184
0.7465


H13
0.7516
0.0158
−0.2576
H51
0.0235
0.0971
0.7859


H14
0.7962
0.1336
−0.2806
H52
0.5928
0.0666
0.5464


H15
0.2748
−0.1368
−0.1257
H53
0.5474
−0.0113
0.4930


H16
0.1354
0.4138
−0.1134
H54
0.6177
−0.0677
0.5491


H17
0.5637
0.4220
−0.3895
H55
−0.3061
0.4584
0.8563


H18
0.3548
−0.0376
−0.1962
H56
−0.2649
0.5278
0.7690


H19
0.6113
0.2344
−0.1012
H57
0.0729
0.2173
0.1548


H20
0.5978
0.2227
−0.0368
H58
0.0660
0.3846
0.1082


H21
0.5035
0.2989
−0.0779
H59
0.1128
0.4405
0.1680


H22
0.3497
0.4290
−0.2687
H60
−0.0185
0.3821
0.1556


H23
0.4036
0.5045
−0.3517
H61
−0.2515
0.3826
0.3034


H24
0.2186
0.7106
−0.0509
H62
−0.3662
0.3282
0.2853


H25
0.1608
0.7135
0.0054
H63
0.4084
0.3542
0.3511


H26
0.0930
0.7757
−0.0497
H64
0.3774
0.1355
0.3316


H27
−0.1018
0.6535
−0.0430
H65
0.4290
0.1920
0.3906


H28
−0.0295
0.5949
0.0121
H66
0.5088
0.1939
0.3414
















TABLE 6







Fractional Atomic Coordinates for the SB-2 Form of Example 3 at 203K














Atom
X
Y
Z
Atom
X
Y
Z

















N1
0.1020
0.3433
0.6995
H33
0.3069
0.0707
0.0797


N2
0.1451
0.2098
0.7726
N6
0.0906
0.5778
−0.1977


C1
0.1521
0.0990
0.4257
N7
0.1038
0.3597
0.2184


N3
0.0666
0.1387
0.3886
N8
0.1832
0.4417
0.3028


O1
−0.0810
0.2000
0.3000
O5
0.1472
0.1787
0.1924


O2
−0.0617
0.4513
0.7014
O6
0.2653
0.4991
−0.1831


O3
0.3455
−0.1959
0.4102
O7
0.0530
0.5367
0.2460


O4
0.2578
0.2270
0.6922
O8
0.4604
−0.0547
0.0595


N4
−0.2237
0.3230
0.3182
F2
0.3424
0.4176
0.3974


F1
0.0929
0.1320
0.8861
C33
0.1488
0.3750
−0.0188


C2
0.0515
0.2481
0.8039
C34
0.1851
0.4059
−0.0748


C3
0.0278
0.2425
0.4073
C35
0.1325
0.4955
−0.1052


C4
0.1352
0.3947
0.6451
C36
0.2185
0.2768
−0.0021


C5
0.0876
0.2715
0.4588
C37
0.0109
0.5268
−0.0219


C6
−0.0583
0.3127
0.3831
C38
0.2931
0.2545
−0.0484


C7
0.1161
0.4086
0.5430
C39
0.1671
0.5246
−0.1644


C8
−0.0236
0.3302
0.7808
C40
0.0613
0.4372
0.0086


C9
0.1733
0.2583
0.7207
C41
0.3037
0.1183
0.0472


C10
0.0002
0.3801
0.7252
C42
0.2346
0.2494
0.2775


C11
−0.0792
0.4148
0.4109
C43
0.0446
0.5543
−0.0770


C12
0.0235
0.2092
0.8599
C44
−0.1499
0.3417
0.1204


C13
0.1685
0.1791
0.4702
C45
0.0317
0.3707
0.1668


C14
−0.0210
0.4457
0.4609
C46
0.1598
0.2563
0.2269


C15
−0.1217
0.2756
0.3314
C47
0.0836
0.4073
0.1161


C16
0.0848
0.3536
0.5942
C48
0.0150
0.4051
0.0659


C17
0.2155
−0.0010
0.4222
C49
0.2951
0.1485
0.2878


C18
0.2117
0.4840
0.6463
C50
0.2252
0.2048
0.0453


C19
0.2403
0.5377
0.5954
C51
0.1092
0.4518
0.2562


C20
−0.1198
0.3713
0.8123
C52
−0.1004
0.3730
0.0688


C21
0.1925
0.4993
0.5443
C53
0.3741
0.1670
−0.0469


C22
0.2525
0.1567
0.5119
C54
0.3862
0.2340
0.3696


C23
0.3707
−0.1318
0.4616
C55
−0.0831
0.3399
0.1697


C24
0.2991
−0.0225
0.4636
C56
0.2060
0.4509
0.1168


C25
0.0011
0.2555
0.5954
C57
0.3254
0.3308
0.3601


C26
0.3168
0.0564
0.5080
C58
0.2470
0.3432
0.3137


C27
0.0614
0.3739
0.4866
C59
0.3800
0.0980
0.0016


C28
0.2163
0.1107
0.7897
C60
0.3714
0.1406
0.3341


C29
−0.0681
0.2470
0.8893
C61
0.4712
0.0047
0.0064


C30
−0.1424
0.3305
0.8659
C62
0.1864
0.5408
0.3416


C31
0.3385
−0.2087
0.5112
C63
0.5902
0.0588
0.0044


C32
0.4991
−0.1042
0.4629
C64
0.4594
−0.0801
−0.0416


N5
0.2714
0.3332
−0.0921
O9
0.6592
0.2259
0.2172


C65
0.5405
0.2502
0.2111
O10
0.4998
0.0747
0.1606


C66
0.5222
0.3739
0.2138
H34
0.0070
0.6146
−0.0961


C67
0.4872
0.1973
0.1604
H35
−0.2287
0.3217
0.1218


O11
0.4252
0.3177
0.8040
H36
0.2841
0.0856
0.2632


O12
0.4975
0.3152
0.6851
H37
0.1750
0.2162
0.0765


C68
0.5426
0.3068
0.7874
H38
−0.1460
0.3725
0.0352


C69
0.5644
0.3351
0.7301
H39
0.4239
0.1549
−0.0780


C70
0.5898
0.1924
0.8075
H40
0.4390
0.2302
0.4007


O13
0.3709
0.0244
0.6545
H41
−0.1155
0.3181
0.2050


H1
0.0414
0.1032
0.3581
H42
0.2590
0.3881
0.1233


H2
0.3817
−0.1684
0.3828
H43
0.2151
0.5064
0.1476


H3
−0.2618
0.3012
0.2876
H44
0.2227
0.4866
0.0802


H4
−0.2521
0.3759
0.3402
H45
0.4125
0.0728
0.3413


H5
−0.1347
0.4650
0.3953
H46
0.1296
0.5966
0.3288


H6
−0.0372
0.5164
0.4780
H47
0.2631
0.5747
0.3411


H7
0.2017
−0.0531
0.3922
H48
0.1687
0.5167
0.3803


H8
0.2441
0.5086
0.6814
H49
0.6017
0.1062
0.0382


H9
0.2918
0.5997
0.5956
H50
0.5959
0.1053
−0.0299


H10
−0.1682
0.4274
0.7960
H51
0.6489
−0.0003
0.0036


H11
0.2123
0.5355
0.5097
H52
0.5198
−0.1372
−0.0379


H12
0.2658
0.2082
0.5422
H53
0.4668
−0.0414
−0.0782


H13
0.0401
0.1886
0.6106
H54
0.3842
−0.1166
−0.0398


H14
−0.0267
0.2400
0.5566
H55
0.6689
0.1560
0.2173


H15
−0.0639
0.2745
0.6197
H56
0.5679
0.0577
0.1687


H16
0.3739
0.0412
0.5361
H57
0.5018
0.2173
0.2452


H17
0.2617
0.0856
0.7572
H58
0.5503
0.4027
0.2503


H18
0.1660
0.0492
0.8019
H59
0.4401
0.3902
0.2096


H19
0.2678
0.1320
0.8211
H60
0.5642
0.4102
0.1828


H20
−0.0830
0.2173
0.9261
H61
0.4046
0.2166
0.1590


H21
−0.2062
0.3574
0.8867
H62
0.5232
0.2282
0.1258


H22
0.2567
−0.2276
0.5084
H63
0.3998
0.3805
0.7933


H23
0.3538
−0.1698
0.5473
H64
0.4554
0.2594
0.6917


H24
0.3842
−0.2779
0.5096
H65
0.5852
0.3644
0.8105


H25
0.5434
−0.1737
0.4578
H66
0.5784
0.4173
0.7298


H26
0.5195
−0.0700
0.4996
H67
0.6391
0.2994
0.7211


H27
0.5167
−0.0513
0.4321
H68
0.5471
0.1674
0.8410


H28
0.3067
0.3359
−0.1249
H69
0.6712
0.2002
0.8176


H29
0.1084
0.5953
−0.2328
H70
0.5810
0.1369
0.7769


H30
0.0225
0.5951
−0.1844
H71
0.3368
0.0852
0.6659


H31
0.4940
−0.0185
0.0854
H72
0.4340
0.0425
0.6372


H32
−0.0480
0.5697
−0.0045
—
—
—
—
















TABLE 7







Fractional Atomic Coordinates for the SE-2 Form of Example 3 at 203 K














Atom
X
Y
Z
Atom
X
Y
Z

















N1
0.2690
0.3492
−0.0891
F1
0.3514
0.4413
0.4010


C1
0.1409
0.3867
−0.0160
F2
0.0923
0.1460
0.8866


C2
0.2150
0.2946
0.0017
N5
0.0597
0.1574
0.3867


C3
−0.0030
0.5370
−0.0211
N6
−0.2480
0.3343
0.3203


C4
0.1782
0.4192
−0.0720
N7
0.1121
0.3629
0.7002


C5
0.0480
0.4486
0.0102
N8
0.1541
0.2292
0.7742


C6
0.2943
0.2735
−0.0443
O5
0.3335
−0.1808
0.4063


C7
0.1273
0.5093
−0.1029
O6
−0.1023
0.2149
0.3000


C8
0.0357
0.5670
−0.0761
O7
−0.0549
0.4703
0.7013


C9
0.3179
0.1482
0.0555
O8
0.2741
0.2492
0.6957


C10
0.3830
0.1938
−0.0416
C33
0.0207
0.2604
0.4056


C11
0.2290
0.2281
0.0516
C34
0.1493
0.1188
0.4237


C12
0.3959
0.1303
0.0090
C35
0.0870
0.2890
0.4570


C13
0.4980
0.0463
0.0143
C36
−0.0687
0.3291
0.3821


O1
0.5032
0.0008
0.0728
C37
−0.0872
0.4313
0.4096


C14
0.6182
0.1082
0.0074
C38
−0.0219
0.4623
0.4599


C15
0.4818
−0.0461
−0.0301
C39
0.0627
0.3909
0.4852


C16
0.1734
0.5410
−0.1606
C40
0.2573
0.1731
0.5105


C17
0.0709
0.4218
0.1197
C41
0.2117
0.0177
0.4206


C18
0.0001
0.4178
0.0679
C42
0.1686
0.1974
0.4682


C19
0.0173
0.3856
0.1709
C43
0.2972
−0.0053
0.4623


C20
−0.1192
0.3851
0.0707
C44
0.3189
0.0738
0.5066


C21
−0.1701
0.3544
0.1228
C45
0.3672
−0.1156
0.4603


C22
0.1955
0.4681
0.1209
C46
0.3343
−0.1831
0.5129


C23
−0.1009
0.3536
0.1733
C47
0.4991
−0.0954
0.4605


N2
0.1014
0.5947
−0.1968
C48
−0.1409
0.2899
0.3313


O2
0.2802
0.5205
−0.1728
C49
0.1457
0.4127
0.6450


N3
0.0935
0.3757
0.2230
C50
0.0935
0.3708
0.5937


N4
0.1820
0.4622
0.3063
C51
0.1985
0.5167
0.5425


C24
0.1004
0.4683
0.2597
C52
0.1215
0.4256
0.5414


C25
0.2392
0.2734
0.2804
C53
0.2234
0.5023
0.6459


C26
0.1552
0.2765
0.2299
C54
0.2494
0.5552
0.5943


C27
0.3069
0.1771
0.2886
C55
0.0102
0.2721
0.5958


C28
0.2503
0.3665
0.3170
C56
0.1861
0.2775
0.7223


C29
0.4041
0.2624
0.3710
C57
0.0544
0.2641
0.8045


C30
0.3352
0.3559
0.3624
C58
−0.0212
0.3473
0.7807


C31
0.3902
0.1720
0.3344
C59
0.0070
0.3983
0.7252


C32
0.1900
0.5626
0.3432
C60
0.0238
0.2235
0.8587


O3
0.0382
0.5511
0.2507
C61
−0.1203
0.3859
0.8101


O4
0.1433
0.2003
0.1959
C62
−0.0733
0.2600
0.8877


C63
−0.1479
0.3414
0.86397
H31
0.3937
−0.1903
0.3863


C64
0.2274
0.1319
0.7926
H32
−0.1449
0.4808
0.3941


O9
0.6211
0.2207
0.2230
H33
−0.0356
0.5328
0.4768


C65
0.5282
0.2668
0.1881
H34
0.2742
0.2237
0.5410


C66
0.5093
0.3861
0.2061
H35
0.1952
−0.0336
0.3904


O10
0.3868
0.0457
0.6545
H36
0.3787
0.0581
0.5348


O11
0.5123
0.3562
0.6907
H37
0.3623
−0.2596
0.5084


C67
0.5352
0.3390
0.7931
H38
0.2480
−0.1831
0.5166


C68
0.5476
0.3657
0.7410
H39
0.3713
−0.1504
0.5475


O12
0.4994
0.3218
0.8483
H40
0.5239
−0.0604
0.4969


H1
0.3045
0.3516
−0.1225
H41
0.5187
−0.0464
0.4284


H2
−0.0655
0.5780
−0.0044
H42
0.5406
−0.1663
0.4562


H3
−0.0011
0.6276
−0.0956
H43
0.2166
0.5531
0.5075


H4
0.3266
0.1051
0.0896
H44
0.2583
0.5271
0.6813


H5
0.4339
0.1824
−0.0732
H45
0.3015
0.6171
0.5942


H6
0.1770
0.2381
0.0829
H46
0.0552
0.2052
0.6061


H7
0.5636
−0.0392
0.0768
H47
−0.0283
0.2619
0.5579


H8
0.6254
0.1670
0.0365
H48
−0.0502
0.2855
0.6248


H9
0.6211
0.1411
−0.0312
H49
−0.1687
0.4424
0.7935


H10
0.6837
0.0555
0.0125
H50
−0.0900
0.2296
0.9244


H11
0.5497
−0.0966
−0.0277
H51
−0.2158
0.3658
0.8839


H12
0.4762
−0.0137
−0.0688
H52
0.2811
0.1118
0.7616


H13
0.4091
−0.0872
−0.0223
H53
0.1755
0.0689
0.8008


H14
−0.1663
0.3840
0.0363
H54
0.2738
0.1511
0.8273


H15
−0.2514
0.3341
0.1238
H55
0.6455
0.1622
0.2076


H16
0.2522
0.4074
0.1268
H56
0.4546
0.2238
0.1931


H17
0.2041
0.5218
0.1524
H57
0.5496
0.2637
0.1470


H18
0.2110
0.5049
0.0841
H58
0.4941
0.3890
0.2475


H19
−0.1339
0.3316
0.2088
H59
0.4412
0.4168
0.1846


H20
0.1273
0.6172
−0.2303
H60
0.5803
0.4296
0.1978


H21
0.0277
0.6079
−0.1873
H61
0.4585
0.0608
0.6441


H22
0.2966
0.1156
0.2634
H62
0.3532
0.1059
0.6667


H23
0.4610
0.2601
0.4016
H63
0.4440
0.3268
0.6901


H24
0.4365
0.1070
0.3404
H64
0.6397
0.3409
0.7359


H25
0.1572
0.5463
0.3811
H65
0.5425
0.4558
0.7453


H26
0.1448
0.6232
0.3249
H66
0.6027
0.3805
0.8196


H27
0.2732
0.5848
0.3479
H67
0.4475
0.3641
0.8070


H28
0.0321
0.1221
0.3561
H68
0.5447
0.2493
0.7977


H29
−0.2911
0.3104
0.2909
H69
0.5039
0.3782
0.8676


H30
−0.2750
0.3873
0.3425
—
—
—
—
















TABLE 8







Fractional Atomic Coordinates for the SC-3 Form of Example 3 at 203 K














Atom
X
Y
Z
Atom
X
Y
Z

















N1
0.1570
0.4213
0.1942
H31
0.8388
0.3944
1.0532


N2
−0.0315
0.2317
0.6493
N5
0.2102
0.5634
0.9042


N3
0.1903
0.4273
0.3190
N6
0.0593
0.5573
0.9433


N4
−0.2863
0.4242
0.6606
N7
0.6971
0.3629
0.9849


O1
0.0906
0.5505
0.2631
O5
0.7930
0.5301
1.1812


O2
−0.1967
0.2858
0.7084
O6
0.1619
0.6931
0.9830


O3
0.1916
−0.0998
0.6914
O7
0.6951
0.1102
0.7641


O4
0.2912
0.3088
0.3759
O8
0.2650
0.4290
0.8319


F1
0.1868
0.3082
0.0627
N8
0.8455
0.4206
1.0964


C1
0.0225
0.3653
0.5762
F2
−0.1174
0.4467
0.9306


C2
0.0168
0.4667
0.5376
C33
0.1441
0.6086
0.9460


C3
−0.0520
0.3337
0.6179
C34
0.5868
0.4989
0.9715


C4
0.1428
0.4718
0.2587
C35
0.6504
0.2448
0.8780


C5
0.2164
0.3292
0.1899
C36
0.5710
0.4167
0.9142


C6
−0.1313
0.4018
0.6225
C37
0.5080
0.4060
0.8554


C7
0.2640
0.2867
0.2506
C38
0.7036
0.5260
1.0714


C8
0.1745
0.4839
0.3863
C39
0.6570
0.6256
1.0860


C9
−0.0606
0.5343
0.5434
C40
0.0419
0.4580
0.9038


C10
−0.1324
0.5027
0.5846
C41
0.5416
0.6001
0.9882


C11
0.1774
0.2547
0.5587
C42
0.2994
0.6226
0.9038


C12
0.2520
0.3394
0.3195
C43
0.6416
0.3354
0.9250


C13
0.0540
0.1946
0.6299
C44
0.3059
0.7128
0.8585


C14
0.1046
0.4447
0.4266
C45
0.1117
0.4091
0.8666


C15
0.1006
0.0953
0.6492
C46
0.4640
0.7342
0.9012


C16
0.1865
0.0756
0.6231
C47
0.5177
0.3168
0.8092


C17
0.2236
0.1560
0.5784
C48
0.3607
0.4829
0.9962


C18
0.3225
0.1931
0.2478
C49
0.3897
0.7698
0.8577


C19
−0.2066
0.3654
0.6673
C50
0.4577
0.6388
0.9454


C20
0.2307
0.5743
0.4063
C51
0.5794
0.6627
1.0460


C21
0.0449
0.3462
0.4013
C52
0.2015
0.4632
0.8646


C22
0.1469
0.5952
0.5101
C53
0.3728
0.5827
0.9479


C23
0.0911
0.5015
0.4906
C54
0.5886
0.2366
0.8194


C24
0.0904
0.2761
0.5848
C55
0.5985
0.1372
0.7680


C25
0.2297
0.2717
0.1249
C56
0.7845
0.4917
1.1194


C26
0.2401
−0.0335
0.6421
C57
0.5557
0.0311
0.7987


C27
0.2159
0.6310
0.4687
C58
0.0973
0.3077
0.8283


C28
0.2850
0.1805
0.1224
C59
0.5559
0.1598
0.6932


C29
0.3313
0.1391
0.1829
C60
−0.0120
0.6151
0.9827


C30
0.3371
−0.0081
0.6733
C61
−0.0587
0.3033
0.8590


C31
0.2432
−0.1074
0.5760
C62
−0.0437
0.4022
0.8984


C32
0.1099
0.4735
0.1320
C63
0.0122
0.2563
0.8247


O9
0.0354
0.7400
0.8156
C64
0.6671
0.4628
1.0129


C65
0.0216
0.6686
0.7680
H32
0.6976
0.1909
0.8862


C66
−0.0656
0.6040
0.7608
H33
0.4597
0.4586
0.8472


C67
0.0918
0.6435
0.7156
H34
0.6795
0.6699
1.1249


O10
0.4880
0.5365
0.6404
H35
0.2546
0.7353
0.8286


C68
0.4148
0.4904
0.6227
H36
0.5202
0.7742
0.9014


C69
0.3315
0.4914
0.6617
H37
0.4752
0.3096
0.7694


C70
0.4115
0.4153
0.5579
H38
0.3054
0.4936
1.0219


O11
0.6355
0.6043
0.5633
H39
0.4144
0.4767
1.0297


O12
0.2797
0.2536
0.7414
H40
0.3546
0.4143
0.9679


H1
−0.0669
0.1956
0.6772
H41
0.3956
0.8325
0.8276


H2
−0.3322
0.4059
0.6859
H42
0.5514
0.7312
1.0578


H3
−0.2919
0.4807
0.6310
H43
0.5614
−0.0316
0.7661


H4
0.1973
−0.0701
0.7314
H44
0.4907
0.0448
0.8054


H5
−0.0646
0.6034
0.5188
H45
0.5878
0.0129
0.8441


H6
−0.1836
0.5513
0.5868
H46
0.1463
0.2752
0.8050


H7
0.2038
0.3068
0.5282
H47
0.5848
0.2261
0.6737


H8
0.0743
0.0426
0.6792
H48
0.4898
0.1730
0.6950


H9
0.2821
0.1424
0.5612
H49
0.5659
0.0948
0.6632


H10
0.3556
0.1666
0.2891
H50
0.0148
0.6815
1.0063


H11
0.2785
0.5969
0.3779
H51
−0.0346
0.5639
1.0179


H12
0.0219
0.3588
0.3525
H52
−0.0631
0.6378
0.9497


H13
−0.0070
0.3392
0.4311
H53
−0.1175
0.2691
0.8559


H14
0.0815
0.2773
0.4042
H54
0.0027
0.1887
0.7989


H15
0.1369
0.6342
0.5524
H55
−0.0992
0.6139
0.8033


H16
0.2528
0.6936
0.4826
H56
−0.0519
0.5245
0.7545


H17
0.2920
0.1448
0.0784
H57
−0.1033
0.6314
0.7197


H18
0.3689
0.0745
0.1805
H58
0.1290
0.7103
0.7087


H19
0.3338
0.0421
0.7138
H59
0.0605
0.6212
0.6706


H20
0.3723
0.0280
0.6375
H60
0.1316
0.5822
0.7335


H21
0.3674
−0.0781
0.6885
H61
0.3302
0.4245
0.6914


H22
0.2723
−0.1792
0.5889
H62
0.2774
0.4920
0.6283


H23
0.2788
−0.0695
0.5412
H63
0.3313
0.5585
0.6913


H24
0.1806
−0.1207
0.5559
H64
0.4545
0.4436
0.5247


H25
0.0724
0.5362
0.1468
H65
0.3492
0.4154
0.5353


H26
0.0704
0.4181
0.1070
H66
0.4287
0.3389
0.5720


H27
0.1555
0.5011
0.1007
H67
0.5897
0.5822
0.5867


H28
0.7439
0.3233
1.0024
H68
0.6577
0.6661
0.5815


H29
0.7221
0.1653
0.7476
H69
0.2607
0.1931
0.7610


H30
0.8928
0.3998
1.1244
H70
0.2753
0.3098
0.7699
















TABLE 9







Fractional Atomic Coordinates for the SD-3 Form of Example 3 at 203 K














Atom
X
Y
Z
Atom
X
Y
Z

















N1
−0.0198
0.1841
0.6526
H39
0.5091
0.1296
0.7031


C1
0.0649
0.1518
0.6275
C37
0.5141
0.3529
0.8608


C2
0.1155
0.0553
0.6439
C38
0.5767
0.3683
0.9162


C3
0.1988
0.0403
0.6124
C39
0.5876
0.4510
0.9710


C4
0.2291
0.1216
0.5658
C40
0.5389
0.5484
0.9893


C5
0.1785
0.2172
0.5490
C41
0.5729
0.6123
1.0451


C6
0.0943
0.2331
0.5800
C42
0.6507
0.5804
1.0822


C7
0.0227
0.3172
0.5753
C43
0.7007
0.4848
1.0665


C8
0.0103
0.4154
0.5359
C44
0.6684
0.4205
1.0092


C9
−0.0684
0.4791
0.5459
C45
0.6099
0.0869
0.7755


C10
−0.1341
0.4455
0.5930
O5
0.7045
0.0545
0.7726


C11
−0.1258
0.3474
0.6318
C46
0.5610
−0.0147
0.8052


C12
−0.0459
0.2826
0.6223
C47
0.5736
0.1104
0.7019


C13
0.2563
−0.0642
0.6258
C48
0.7841
0.4475
1.1068


O1
0.2137
−0.1294
0.6803
O6
0.8348
0.3739
1.0850


C14
0.2572
−0.1377
0.5619
N6
0.8009
0.4968
1.1686


C15
0.3529
−0.0333
0.6508
C49
0.4556
0.5857
0.9502


C16
−0.1945
0.3094
0.6830
C50
0.3721
0.5266
0.9541


O2
−0.1818
0.2255
0.7187
C51
0.2977
0.5689
0.9157


N2
−0.2704
0.3724
0.6902
C52
0.3032
0.6637
0.8746


C17
0.0802
0.4535
0.4854
C53
0.3855
0.7216
0.8718


C18
0.0906
0.3957
0.4215
C54
0.4606
0.6827
0.9098


C19
0.1577
0.4370
0.3780
C55
0.3620
0.4248
0.9988


C20
0.2001
0.5839
0.4566
N7
0.2092
0.5129
0.9187


C21
0.2127
0.5281
0.3946
C56
0.1439
0.5646
0.9613


C22
0.1334
0.5461
0.5016
N8
0.0559
0.5258
0.9558


C23
0.0321
0.2976
0.4013
C57
0.0317
0.4338
0.9143


N3
0.1714
0.3822
0.3110
C58
−0.0595
0.3969
0.9029


C24
0.1220
0.4251
0.2538
C59
−0.0805
0.3043
0.8644


N4
0.1390
0.3807
0.1900
C60
−0.0124
0.2436
0.8338


C25
0.2019
0.2947
0.1812
C61
0.0782
0.2781
0.8404


C26
0.2190
0.2429
0.1168
C62
0.0999
0.3739
0.8799


C27
0.2780
0.1563
0.1099
C63
0.1938
0.4164
0.8800


C28
0.3260
0.1144
0.1673
O7
0.2558
0.3752
0.8471


C29
0.3129
0.1632
0.2314
O8
0.1670
0.6400
1.0005


C30
0.2511
0.2525
0.2388
C64
−0.0123
0.5867
0.9965


C31
0.2370
0.2991
0.3072
F2
−0.1297
0.4555
0.9291


O3
0.2776
0.2680
0.3603
O9
−0.0315
0.6352
0.8142


O4
0.0646
0.4983
0.2621
C65
0.0467
0.6974
0.7957


C32
0.0891
0.4341
0.1312
C66
0.1015
0.6235
0.7479


F1
0.1750
0.2791
0.0583
C67
0.0324
0.5369
0.7208


N5
0.7040
0.3249
0.9806
C68
−0.0545
0.5713
0.7529


C33
0.6498
0.2923
0.9241
O10
0.3335
0.6673
0.6781


C34
0.6618
0.2025
0.8794
C69
0.4290
0.6547
0.6901


C35
0.5996
0.1879
0.8240
C70
0.4507
0.5461
0.7165


C36
0.5262
0.2635
0.8159
C71
0.3638
0.4820
0.7010


C73
0.4496
0.4500
0.4861
C72
0.3053
0.5567
0.6543


O11
0.5228
0.4477
0.5332
H40
0.5814
0.0441
0.6734


C74
0.4039
0.3419
0.4916
H41
0.8483
0.4767
1.1939


C75
0.4679
0.2645
0.5269
H42
0.7643
0.5489
1.1832


C76
0.5347
0.3365
0.5613
H43
0.2516
0.6888
0.8487


O12
0.2870
0.1954
0.7523
H44
0.3902
0.7866
0.8443


O13
0.3474
0.0553
0.3973
H45
0.5162
0.7225
0.9085


H1
−0.0511
0.1471
0.6828
H46
0.3150
0.4382
1.0324


H2
0.0937
0.0021
0.6756
H47
0.4198
0.4092
1.0229


H3
0.2860
0.1111
0.5450
H48
0.3448
0.3610
0.9699


H4
0.2007
0.2701
0.5173
H49
−0.1420
0.2817
0.8586


H5
−0.0773
0.5459
0.5205
H50
−0.0273
0.1784
0.8083


H6
−0.1860
0.4910
0.5988
H51
0.1247
0.2376
0.8186


H7
0.2210
−0.1974
0.6727
H52
0.0188
0.6398
1.0272


H8
0.2925
−0.2052
0.5718
H53
−0.0466
0.5335
1.0238


H9
0.2847
−0.0969
0.5241
H54
−0.0541
0.6268
0.9652


H10
0.1948
−0.1583
0.5485
H55
0.0835
0.7171
0.8372


H11
0.3500
0.0056
0.6949
H56
0.0283
0.7670
0.7719


H12
0.3808
0.0155
0.6168
H57
0.1524
0.5871
0.7733


H13
0.3893
−0.1010
0.6563
H58
0.1262
0.6675
0.7096


H14
−0.3110
0.3535
0.7204
H59
0.0268
0.5392
0.6700


H15
−0.2788
0.4320
0.6645
H60
0.0502
0.4609
0.7354


H16
0.2363
0.6468
0.4684
H61
−0.0912
0.6170
0.7203


H17
0.2582
0.5518
0.3642
H62
−0.0903
0.5048
0.7651


H18
0.1247
0.5843
0.5437
H63
0.4506
0.7117
0.7235


H19
−0.0080
0.3181
0.3622
H64
0.4609
0.6666
0.6466


H20
−0.0047
0.2756
0.4402
H65
0.4647
0.5485
0.7666


H21
0.0709
0.2352
0.3881
H66
0.5027
0.5131
0.6926


H22
0.2865
0.1243
0.0659
H67
0.3321
0.4653
0.7440


H23
0.3667
0.0538
0.1628
H68
0.3774
0.4111
0.6775


H24
0.3458
0.1364
0.2706
H69
0.3190
0.5453
0.6052


H25
0.0511
0.4944
0.1483
H70
0.2398
0.5441
0.6612


H26
0.0507
0.3786
0.1076
H71
0.4713
0.4612
0.4387


H27
0.1327
0.4644
0.0989
H72
0.4075
0.5113
0.4970


H28
0.7532
0.2902
0.9956
H73
0.3868
0.3132
0.4454


H29
0.7110
0.1524
0.8863
H74
0.3480
0.3497
0.5185


H30
0.4839
0.2530
0.7788
H75
0.4362
0.2180
0.5608


H31
0.4645
0.4025
0.8541
H76
0.4974
0.2152
0.4933


H32
0.5421
0.6785
1.0576
H77
0.5254
0.3370
0.6115


H33
0.6708
0.6254
1.1197
H78
0.5969
0.3093
0.5530


H34
0.7350
0.1077
0.7576
H79
0.2609
0.2108
0.7129


H35
0.5709
−0.0795
0.7758
H80
0.2777
0.2494
0.7810


H36
0.4958
0.0007
0.8071
H81
0.3954
0.0411
0.4226


H37
0.5851
−0.0301
0.8517
H82
0.3403
0.1269
0.3936


H38
0.6074
0.1726
0.6823
—
—
—
—
















TABLE 10







Fractional Atomic Coordinates for the M2-4 Form of Example 3 at 203 K














Atom
X
Y
Z
Atom
X
Y
Z

















N1
0.5372
0.4291
0.1019
C32
0.9325
−0.0002
0.1354


N2
0.5726
0.5600
0.0850
N5
0.4286
−0.0679
0.1199


N3
0.6996
0.0893
−0.0280
N6
0.5674
−0.1011
0.0667


N4
0.5416
0.1538
−0.1474
N7
0.4644
0.1942
0.3210


O1
0.6733
0.3589
0.1382
N8
0.7855
0.1073
0.3824


O2
0.4046
0.4982
0.0630
O5
0.0087
0.3111
0.2331


O3
0.6342
0.0545
−0.1153
O6
0.2677
0.0059
0.1067


O4
1.0378
0.0176
0.0699
O7
0.6582
0.2053
0.3727


F1
0.7255
0.6914
0.0979
O8
0.5853
−0.1473
0.1332


C1
0.5589
0.1621
0.0046
F2
0.6201
−0.0991
−0.0223


C2
0.6463
0.1387
0.0356
C33
0.1846
0.2541
0.2669


C3
0.7323
0.0946
0.0139
C34
0.5188
0.1246
0.3138


C4
0.6422
0.4216
0.1244
C35
0.4896
0.0118
0.2693


C5
0.5320
0.1444
−0.0718
C36
0.2813
0.2601
0.2944


C6
0.4303
0.2531
0.0462
C37
0.3916
−0.0268
0.0484


C7
0.5955
0.1301
−0.0344
C38
0.4965
−0.0642
0.0361


C8
0.8337
0.0653
0.0339
C39
0.3975
−0.0728
0.1644


C9
0.3932
0.2216
−0.0303
C40
0.4262
−0.0334
0.2359


C10
0.8501
0.0815
0.0766
C41
0.6548
0.0200
0.3192


C11
0.4690
0.3595
0.0923
C42
0.4566
−0.0253
0.1931


C12
0.4288
0.1889
−0.0685
C43
0.3635
0.2005
0.2959


C13
0.4935
0.3219
0.0544
C44
0.3174
0.0094
0.0191


C14
0.4983
0.4971
0.0822
C45
0.3554
−0.0278
0.0930


C15
0.3461
0.2264
0.0749
C46
0.6216
0.0934
0.3325


C16
0.7623
0.1235
0.0988
C47
0.3524
0.1339
0.2711


C17
0.4586
0.2101
0.0065
C48
0.5217
−0.0634
−0.0072


C18
0.3857
0.3326
0.1209
C49
0.3406
−0.0863
0.2481


C19
0.3236
0.2655
0.1119
C50
0.5909
−0.0201
0.2887


C20
0.7526
0.6232
0.1152
C51
0.3456
0.0090
−0.0228


C21
0.5738
0.1130
−0.1130
C52
0.5310
−0.1085
0.1074


C22
0.6768
0.5593
0.1095
C53
0.4526
0.0844
0.2826


C23
0.7134
0.4916
0.1297
C54
0.4490
−0.0283
−0.0361


C24
0.6617
0.1520
0.0790
C55
0.2568
0.1284
0.2432


C25
0.8171
0.4875
0.1533
C56
0.6899
0.1387
0.3640


C26
0.9628
0.0558
0.0999
C57
0.1745
0.1888
0.2411


C27
0.5853
0.3524
0.0242
C58
0.3125
−0.1258
0.1764


C28
0.8546
0.6191
0.1387
C59
0.0875
0.3163
0.2685


C29
0.8880
0.5516
0.1582
C60
0.2825
−0.1321
0.2190


C30
1.0293
0.1252
0.1175
C61
0.5506
0.0308
0.1786


C31
0.5394
0.6268
0.0584
C62
0.0172
0.3085
0.3092


C63
0.1406
0.3971
0.2645
H31
−0.0321
0.2713
0.2351


C64
0.6840
−0.1358
0.0557
H32
0.2906
0.3038
0.3116


O9
0.6489
0.2289
0.1856
H33
0.7226
−0.0033
0.3313


C65
0.5443
0.2369
0.2097
H34
0.2474
0.0343
0.0283


O10
0.8593
0.1883
0.2209
H35
0.3215
−0.0911
0.2769


C66
0.8410
0.1261
0.2471
H36
0.6160
−0.0698
0.2808


O11
0.7082
0.3027
0.4362
H37
0.2956
0.0337
−0.0425


C67
0.6814
0.2742
0.4743
H38
0.4689
−0.0294
−0.0650


O12
0.9373
0.2494
0.4177
H39
0.2477
0.0847
0.2259


C68
0.9929
0.3124
0.4391
H40
0.1104
0.1855
0.2219


H1
0.7386
0.0640
−0.0473
H41
0.2752
−0.1572
0.1562


H2
0.5640
0.1387
−0.1724
H42
0.2234
−0.1672
0.2278


H3
0.4985
0.1952
−0.1446
H43
0.6220
0.0028
0.1703


H4
1.1052
0.0112
0.0803
H44
0.5699
0.0659
0.2015


H5
0.8895
0.0353
0.0189
H45
0.5207
0.0599
0.1546


H6
0.3236
0.2520
−0.0294
H46
−0.0431
0.3487
0.3106


H7
0.3819
0.1972
−0.0927
H47
0.0710
0.3134
0.3331


H8
0.3038
0.1808
0.0689
H48
−0.0214
0.2584
0.3101


H9
0.7727
0.1323
0.1279
H49
0.1799
0.4022
0.2373


H10
0.3714
0.3595
0.1462
H50
0.1983
0.4053
0.2869


H11
0.2662
0.2468
0.1310
H51
0.0772
0.4351
0.2667


H12
0.6043
0.1799
0.0944
H52
0.7256
−0.1503
0.0814


H13
0.8394
0.4407
0.1661
H53
0.7317
−0.0987
0.0401


H14
0.5698
0.4065
0.0187
H54
0.6712
−0.1812
0.0384


H15
0.5812
0.3238
−0.0021
H55
0.6553
0.2663
0.1693


H16
0.6644
0.3467
0.0364
H56
0.5545
0.2109
0.2366


H17
0.9028
0.6632
0.1416
H57
0.4775
0.2142
0.1947


H18
0.9582
0.5494
0.1745
H58
0.5286
0.2912
0.2146


H19
1.1035
0.1082
0.1305
H59
0.7953
0.2007
0.2095


H20
0.9801
0.1507
0.1385
H60
0.9150
0.0973
0.2499


H21
1.0471
0.1609
0.0948
H61
0.7798
0.0930
0.2350


H22
0.4734
0.6127
0.0400
H62
0.8152
0.1440
0.2746


H23
0.6076
0.6420
0.0413
H63
0.6689
0.2796
0.4178


H24
0.5157
0.6695
0.0763
H64
0.7099
0.3093
0.4960


H25
0.8947
−0.0457
0.1237
H65
0.7195
0.2244
0.4778


H26
0.8782
0.0245
0.1551
H66
0.5953
0.2684
0.4768


H27
1.0052
−0.0149
0.1500
H67
0.8638
0.2544
0.4197


H28
0.4895
0.2290
0.3387
H68
0.9414
0.3299
0.4619


H29
0.8263
0.1335
0.4008
H69
1.0060
0.3543
0.4193


H30
0.8068
0.0604
0.3760
H70
1.0691
0.2959
0.4506
















TABLE 11







Fractional Atomic Coordinates for the AN-5 Form of Example 3 at 203 K














Atom
X
Y
Z
Atom
X
Y
Z

















N1
0.9924
0.6814
0.0947
C29
0.4287
0.2860
0.2241


C1
1.0444
0.8258
0.1951
C30
0.2467
0.5974
0.7121


C2
1.0161
0.7433
0.1389
C31
−0.1039
0.0566
0.5110


N2
0.2704
0.1624
0.4096
C32
0.3565
0.2030
0.1964


N3
−0.0856
0.3512
0.6256
C33
0.4392
0.3250
0.2896


N4
0.3894
0.3101
0.3969
C34
0.4583
0.4081
0.4282


N5
−0.3954
0.1970
0.5396
F1
0.5134
0.4072
0.3147


O1
−0.2963
0.3045
0.6238
N6
0.2289
0.5532
0.0900


O2
0.1787
0.0573
0.3196
N7
0.5870
0.3648
−0.1210


O3
0.3553
0.5578
0.7487
N8
0.2074
0.5299
0.1999


O4
0.3596
0.2732
0.4995
O5
0.3183
0.6700
0.1776


C3
0.3036
0.1895
0.2999
O6
0.1487
0.1663
−0.2511


C4
0.2207
0.1026
0.4556
O7
0.1375
0.4413
0.0027


C5
0.0214
0.3845
0.6351
C35
0.6077
0.4649
−0.0814


C6
0.1428
−0.0166
0.5477
C36
0.3230
0.2303
−0.1785


C7
0.1268
0.1400
0.4661
C37
0.2557
0.1306
−0.2138


C8
0.0878
0.0774
0.5149
C38
0.3687
0.5793
0.0337


C9
0.2336
0.4103
0.6439
C39
0.1457
0.4328
0.1807


C10
0.3783
0.2770
0.3299
C40
0.4083
0.6392
−0.0146


C11
0.3417
0.2516
0.4390
C41
0.2664
0.3150
−0.1487


C12
0.0763
0.4797
0.6715
C42
0.1202
0.3970
0.1125


C13
0.0710
0.2412
0.4289
C43
0.4283
0.2447
−0.1714


C14
0.0696
0.3046
0.6030
C44
0.3527
0.7332
−0.0501


C15
−0.0111
0.2197
0.5704
C45
0.4300
0.4229
−0.1029


C16
0.2697
0.6789
0.6598
C46
0.5103
0.5026
−0.0711


C17
−0.2038
0.1910
0.5624
C47
0.4308
0.4781
0.0734


C18
0.1798
0.3155
0.6093
C48
0.7037
0.5215
−0.0556


C19
0.2459
0.1309
0.3417
C49
0.1023
0.3670
0.2238


C20
−0.1068
0.2516
0.5868
C50
0.2162
0.7098
0.0072


C21
−0.0097
0.1194
0.5318
C51
0.6074
0.6611
−0.0035


C22
0.1846
0.4911
0.6758
C52
0.2327
0.0386
−0.1643


C23
0.2767
0.0071
0.4870
C53
0.0612
0.2994
0.0903


C24
0.2368
−0.0556
0.5365
C54
0.3045
0.0704
−0.2675


C25
−0.3011
0.2350
0.5771
C55
0.4819
0.3364
−0.1343


C26
0.2975
0.1513
0.2349
C56
0.5106
0.6026
−0.0292


C27
0.1973
0.6564
0.7593
C57
0.7010
0.6216
−0.0164


C28
−0.1980
0.0929
0.5244
C58
0.2553
0.7676
−0.0395


C59
0.3212
0.4044
−0.1104
H24
−0.2605
0.0489
0.5069


C60
0.0456
0.2675
0.2020
H25
0.4722
0.3176
0.1985


C61
0.2545
0.5879
0.1583
H26
−0.1042
−0.0131
0.4870


C62
0.2729
0.6182
0.0426
H27
0.3467
0.1807
0.1508


C63
0.1595
0.4650
0.0629
H28
0.5324
0.3890
0.4318


C64
0.0194
0.2342
0.1342
H29
0.4499
0.4229
0.4733


C65
0.2320
0.5767
0.2708
H30
0.4380
0.4770
0.4002


F2
0.1269
0.3898
0.2910
H31
0.6344
0.3260
−0.1352


C66
0.8052
0.4782
−0.0676
H32
0.1523
0.2136
−0.2812


O8
0.8000
0.4141
−0.1178
H33
0.1921
0.3084
−0.1556


N9
0.8982
0.5101
−0.0263
H34
0.4653
0.1918
−0.1920


N10
−0.4881
0.0375
0.4096
H35
0.3808
0.7741
−0.0813


C67
−0.5475
−0.0890
0.2994
H36
0.4027
0.4056
0.0513


C68
−0.5145
−0.0213
0.3629
H37
0.4236
0.4788
0.1196


H1
0.9974
0.8929
0.1843
H38
0.5057
0.4852
0.0746


H2
1.1180
0.8506
0.2021
H39
0.1510
0.7326
0.0150


H3
1.0364
0.7890
0.2362
H40
0.6092
0.7284
0.0229


H4
−0.1316
0.3880
0.6419
H41
0.1960
0.0751
−0.1342


H5
−0.4539
0.2212
0.5481
H42
0.2997
0.0054
−0.1376


H6
−0.3985
0.1480
0.5067
H43
0.1880
−0.0224
−0.1902


H7
0.3557
0.5357
0.7875
H44
0.0488
0.2761
0.0447


H8
0.1152
−0.0565
0.5794
H45
0.2533
0.0152
−0.2940


H9
0.3062
0.4204
0.6458
H46
0.3696
0.0300
−0.2441


H10
0.0412
0.5340
0.6923
H47
0.3205
0.1288
−0.2976


H11
0.1218
0.3039
0.4311
H48
0.7648
0.6631
0.0017


H12
0.0156
0.2664
0.4494
H49
0.2168
0.8300
−0.0643


H13
0.0389
0.2202
0.3817
H50
0.2843
0.4550
−0.0885


H14
0.3070
0.7471
0.6825
H51
0.0239
0.2208
0.2336


H15
0.2029
0.7025
0.6279
H52
−0.0250
0.1698
0.1184


H16
0.3140
0.6397
0.6354
H53
0.2810
0.6418
0.2753


H17
0.2159
0.2594
0.5904
H54
0.2647
0.5161
0.3029


H18
0.3396
−0.0171
0.4765
H55
0.1663
0.6025
0.2801


H19
0.2735
−0.1202
0.5600
H56
0.9575
0.4863
−0.0338


H20
0.2522
0.0887
0.2165
H57
0.9001
0.5548
0.0085


H21
0.2471
0.7127
0.7859
H58
−0.6241
−0.0805
0.2796


H22
0.1791
0.5998
0.7895
H59
−0.5305
−0.1704
0.3092


H23
0.1329
0.6961
0.7337
H60
−0.5100
−0.0607
0.2675
















TABLE 12







Fractional Atomic Coordinates for the H1-6 Form of Example 3


at Room Temperature














Atom
X
Y
Z
Atom
X
Y
Z

















N1
0.6135
0.3022
0.1291
C31
−0.0330
0.1910
0.5041


C1
0.4861
0.2699
0.1329
O3
0.2583
0.6097
0.4356


C2
0.5140
0.4378
0.1846
O4
0.0757
0.3581
0.3067


C3
0.6315
0.4018
0.1603
C32
0.1334
0.5294
0.5329


C4
0.2928
0.3371
0.1767
F1
0.0468
0.3260
0.5710


C5
0.7320
0.5647
0.2046
O5
0.0547
0.2775
0.0276


C6
0.5076
0.5372
0.2202
H1
0.6712
0.2650
0.1101


C7
0.7432
0.4642
0.1716
H2
0.2472
0.3892
0.1998


C8
0.6172
0.5979
0.2282
H3
0.8030
0.6105
0.2108


C9
0.2279
0.0573
0.0899
H4
0.6141
0.6650
0.2510


C10
0.2980
0.1613
0.1160
H5
0.4721
0.1244
0.0847


C11
0.4263
0.1768
0.1076
H6
0.1480
0.2331
0.1574


C12
0.4222
0.3523
0.1670
H7
0.0768
0.1355
0.0730


C13
0.2343
0.2429
0.1511
H8
0.2225
−0.0310
0.0119


O1
0.0939
0.0773
0.0920
H9
0.3587
0.0122
0.0281


C14
0.8643
0.4211
0.1477
H10
0.2580
0.0991
0.0032


C15
0.2706
0.0322
0.0277
H11
0.2049
−0.0285
0.1687


C16
0.2430
−0.0453
0.1308
H12
0.3313
−0.0611
0.1363


O2
0.8692
0.3628
0.1024
H13
0.2025
−0.1111
0.1134


N2
0.9694
0.4487
0.1781
H14
1.0421
0.4253
0.1657


C17
0.1540
0.6355
0.2977
H15
0.9643
0.4898
0.2101


C18
0.3384
0.5117
0.2978
H16
0.0771
0.6566
0.3146


C19
0.2228
0.5440
0.3210
H17
0.4163
0.4214
0.3666


C20
0.4078
0.4106
0.3243
H18
0.4902
0.4048
0.3066


C21
0.3158
0.6634
0.2252
H19
0.3612
0.3411
0.3167


C22
0.3847
0.5718
0.2479
H20
0.3480
0.7048
0.1931


C23
0.2007
0.6947
0.2493
H21
0.1550
0.7554
0.2328


N3
0.1708
0.4804
0.3708
H22
−0.0158
0.1842
0.3610


C24
0.1923
0.5250
0.4285
H23
−0.0940
0.0839
0.4407


N4
0.1385
0.4665
0.4747
H24
−0.0618
0.1478
0.5364


C25
0.0982
0.3838
0.3581
H25
0.1867
0.4912
0.5615


C26
0.0546
0.3187
0.4093
H26
0.0479
0.5303
0.5474


C27
−0.0062
0.2128
0.3995
H27
0.1623
0.6071
0.5273


C28
0.0269
0.2925
0.5133
H28
0.0015
0.3180
0.0477


C29
0.0754
0.3609
0.4673
H29
0.0981
0.3219
0.0046


C30
−0.0507
0.1524
0.4471
—
—
—
—
















TABLE 13







Fractional Atomic Coordinates for the E-7 Form of Example 3


at Room Temperature














Atom
X
Y
Z
Atom
X
Y
Z

















O1
0.6824
0.3544
−0.0493
O4
0.6263
0.4869
0.1391


N1
0.4205
−0.0993
0.0594
F1
0.3561
0.6987
0.0032


C1
0.5324
0.0321
0.0919
O5
0.5820
0.2157
−0.2431


C2
0.5663
0.0175
0.0275
C33
0.8341
0.1921
−0.2520


C3
0.5679
0.1009
0.1351
C34
0.7180
0.2426
−0.2245


C4
0.4436
−0.0426
0.1097
H1
0.3690
−0.1486
0.0592


C5
0.5164
0.0920
0.1944
H2
0.5391
0.1372
0.2236


C6
0.4996
−0.0984
−0.0510
H3
0.4497
−0.1516
−0.0622


C7
0.3931
−0.0524
0.1701
H4
0.6981
0.1165
−0.0051


C8
0.6483
0.0631
−0.0159
H5
0.3995
0.0145
0.2520


C9
0.4317
0.0177
0.2115
H6
0.7110
0.0591
−0.1039


C10
0.4935
−0.0646
0.0087
H7
0.6082
−0.2139
−0.1504


C11
0.5823
−0.0841
−0.1616
H8
0.7912
−0.1056
−0.1717


C12
0.6552
0.0284
−0.0752
H9
0.7449
−0.0051
−0.1927


C13
0.5815
−0.0516
−0.0940
H10
0.7148
−0.0919
−0.2351


O2
0.5447
−0.1820
−0.1647
H11
0.4638
−0.0550
−0.2376


C14
0.3048
−0.1336
0.1871
H12
0.4842
0.0326
−0.1948


C15
0.7209
−0.0704
−0.1931
H13
0.3794
−0.0467
−0.1758


C16
0.4669
−0.0337
−0.1955
H14
0.2148
−0.1898
0.2567


O3
0.2688
−0.1909
0.1469
H15
0.2921
−0.1026
0.2729


N2
0.2660
−0.1431
0.2459
H16
0.8965
0.3781
0.0762


C17
0.6970
0.3338
0.0749
H17
0.9840
0.2455
0.1237


C18
0.6598
0.1816
0.1177
H18
0.8374
0.1249
0.1501


C19
0.6044
0.2621
0.0886
H19
0.4180
0.3287
0.0884


C20
0.8376
0.3289
0.0869
H20
0.4033
0.2194
0.0921


C21
0.8893
0.2501
0.1151
H21
0.4415
0.2674
0.0292


C22
0.8013
0.1777
0.1306
H22
0.5777
0.4563
−0.1337


C23
0.4533
0.2701
0.0732
H23
0.3388
0.6831
−0.1114


N3
0.6426
0.4177
0.0452
H24
0.4452
0.5759
−0.1760


N4
0.5461
0.5682
0.0566
H25
0.4493
0.6677
0.1067


C24
0.6057
0.4911
0.0838
H26
0.5777
0.7063
0.0699


C25
0.6371
0.4197
−0.0189
H27
0.6008
0.6468
0.1300


C26
0.5645
0.5001
−0.0452
H28
0.5538
0.2519
−0.2697


C27
0.5108
0.5688
−0.0062
H29
0.8006
0.1353
−0.2706


C28
0.5396
0.5022
−0.1083
H30
0.9012
0.1771
−0.2206


C29
0.3972
0.6374
−0.0950
H31
0.8772
0.2306
−0.2830


C30
0.4217
0.6349
−0.0335
H32
0.7242
0.2357
−0.1800


C31
0.4584
0.5725
−0.1335
H33
0.7295
0.3087
−0.2337


C32
0.5432
0.6548
0.0941
—
—
—
—
















TABLE 14







Fractional Atomic Coordinates for the SE-8 Form of Example 3 at 203 K














Atom
X
Y
Z
Atom
X
Y
Z

















C1
0.6880
0.4895
0.0251
F1
0.8275
0.6234
0.0070


C2
0.7552
0.5380
0.0028
O5
0.7464
0.6231
0.2276


C3
0.7467
0.4943
−0.0255
C33
0.6902
0.5401
0.2462


C4
0.6708
0.4021
−0.0316
C34
0.7150
0.5706
0.2775


C5
0.6035
0.3535
−0.0093
O6
0.6752
0.7322
0.1413


C6
0.6121
0.3972
0.0191
H1
0.7921
0.5273
−0.0406


C7
0.3643
0.2900
0.1269
H2
0.6650
0.3726
−0.0507


C8
0.4170
0.3504
0.1039
H3
0.5522
0.2911
−0.0133


C9
0.5266
0.3158
0.0938
H4
0.6573
0.1972
0.0996


C10
0.5832
0.2207
0.1065
H5
0.5688
0.0960
0.1380


C11
0.5306
0.1603
0.1294
H6
0.3854
0.1541
0.1551


C12
0.4210
0.1950
0.1396
H7
−0.0332
0.2640
0.1374


C13
0.1300
0.4652
0.1674
H8
0.1482
0.1999
0.1202


C14
0.0286
0.4043
0.1600
H9
0.4867
0.4668
0.1548


C15
0.0354
0.3052
0.1424
H10
0.5825
0.6252
0.1764


C16
0.1436
0.2669
0.1321
H11
0.2732
0.7447
0.2073


C17
0.2450
0.3278
0.1395
H12
0.0862
0.6034
0.1927


C18
0.2382
0.4269
0.1571
H13
0.6506
0.7546
0.2134


C19
0.3228
0.5112
0.1682
H14
0.5057
0.9864
0.1989


C20
0.4437
0.5229
0.1654
H15
0.3763
0.9376
0.1959


C21
0.5007
0.6173
0.1783
H16
0.4691
0.9135
0.1708


C22
0.4371
0.7002
0.1939
H17
0.4861
0.7392
0.2492


C23
0.3163
0.6886
0.1967
H18
0.3970
0.8441
0.2444


C24
0.2591
0.5942
0.1838
H19
0.5323
0.8703
0.2489


N1
0.5788
0.3772
0.0703
H20
−0.2559
0.4447
0.1644


C25
0.6524
0.4709
0.0765
H21
−0.1866
0.3825
0.1410


N2
0.6947
0.5340
0.0536
H22
0.3953
0.5254
0.0939


C26
0.5487
0.3391
0.0424
H23
0.2744
0.4594
0.0983


N3
0.1422
0.5653
0.1839
H24
0.3468
0.4413
0.0690


C27
0.4954
0.8081
0.2072
H25
0.7654
0.6513
0.0825


O1
0.6204
0.8022
0.2018
H26
0.7185
0.7116
0.0535


C28
−0.0859
0.4524
0.1712
H27
0.8383
0.6413
0.0531


C29
0.4582
0.9219
0.1918
H28
0.7944
0.5848
0.2177


C30
0.4759
0.8162
0.2405
H29
0.7195
0.4611
0.2419


N4
−0.1888
0.4229
0.1571
H30
0.6054
0.5410
0.2426


O2
−0.0947
0.5096
0.1939
H31
0.7976
0.5861
0.2800


C31
0.3525
0.4536
0.0900
H32
0.6917
0.5056
0.2900


O3
0.4758
0.2620
0.0383
H33
0.6700
0.6399
0.2829


O4
0.6661
0.4992
0.1019
H34
0.6529
0.7372
0.1591


C32
0.7600
0.6443
0.0614
H35
0.6645
0.6624
0.1349

## Claims

1. A compound of Formula (I):



or a salt thereof, wherein:
Q is:




R1 is —C(CH3)2OH, —NHC(═O)C(CH3)3, —N(CH3)2, or —CH2Rd;
R2 is Cl or —CH3;
R3 is H, F, or —CH3;
Ra is H or —CH3;
Rb is H, F, Cl, or —OCH3;
Rc is H or F; and
Rd is —OH, —OCH3, —NHC(═O)CH3, or

2. The compound according to claim 1 or a salt thereof, wherein:
Q is:

3. The compound according to claim 1 or a salt thereof, wherein:
Ra is —CH3;
Rb is F, Cl, or —OCH3; and
Rc is F.

4. The compound according to claim 1 or a salt thereof, wherein:
R2 is —CH3.

5. The compound according to claim 1 wherein:
Q is:





and
R1 is C(CH3)2OH.

6. The compound according to claim 1 or a salt thereof, wherein:
Q is:




R2 is —CH3; and
R3 is H.

7. The compound according to claim 1 wherein said compound is selected from: 4-(3-(8-fluoro-2,4-dioxo-1,2-dihydroquinazolin-3 (4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (1 and 2); 4-(3-(S)-(8-fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3 (4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (3); 4-(3-(S)-(8-fluoro-1-methyl (d3)-2,4-dioxo-1,2-dihydroquinazolin-3 (4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carb azole-1-carboxamide (4); 4-(2-chloro-3-(8-fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3 (4H)-yl)phenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (5); 4-(2-chloro-3-(1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3 (4H)-yl)phenyl)-7-(2-hydroxypropan-2-yl)-9H-carb azole-1-carboxamide (6); 7-(2-hydroxypropan-2-yl)-4-(3-(8-methoxy-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3 (4H)-yl)-2-methylphenyl)-9H-carbazole-1-carboxamide (7); 4-(3-(6-fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carb azole-1-carboxamide (8); 4-(3-(3-(4-fluorophenyl)-2,6-dioxo-2,3-dihydropyrimidin-1(6H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (9); 7-(2-hydroxypropan-2-yl)-4-(3-(7-methoxy-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-9H-carbazole-1-carboxamide (10); 7-(2-hydroxypropan-2-yl)-4-(3-(6-methoxy-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-9H-carbazole-1-carboxamide (11); 7-(2-hydroxypropan-2-yl)-4-(3-(5-methoxy-1,3-dioxo-1H-pyrido[1,2-c] pyrimidin-2(3H)-yl)-2-methylphenyl)-9H-carbazole-1-carboxamide (12); 4-(3-(5-chloro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (13); 4-(3-(R)-(5-chloro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (14); 4-(3-(S)-(5-chloro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (15); 4-(3-(5-chloro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-pivalamido-9H-carbazole-1-carboxamide (16); 4-(3-(5-chloro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(methoxymethyl)-9H-carbazole-1-carboxamide (17); 4-(3-(5-fluoro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(methoxymethyl)-9H-carbazole-1-carboxamide (18); 4-(3-(4-fluoro-1,3-dioxo-1H-pyrido[1,2-c] pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (19); 4-(3-(5,7-dioxo-5H-thiazolo[3,2-c]pyrimidin-6(7H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (20); 4-(3-(5-fluoro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (21); 4-(3-(5-fluoro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (22 and 23); 4-(3-(S)-(8-fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-5-methyl-9H-carbazole-1-carboxamide (24); 4-(3-(8-fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-5-methyl-9H-carbazole-1-carboxamide (25); 4-(3-(S)-(8-fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-8-methyl-9H-carbazole-1-carboxamide (26); 4-(3-(S)-(8-fluoro-1-methyl(d3)-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-5-methyl-9H-carbazole-1-carboxamide (27); 4-(3-(5-fluoro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(hydroxymethyl)-9H-carbazole-1-carboxamide (28); 7-(dimethylamino)-4-(3-(5-fluoro-1,3-dioxo-1H-pyrido[1,2-c] pyrimidin-2(3H)-yl)-2-methylphenyl)-9H-carbazole-1-carboxamide (29); 7-(acetamidomethyl)-4-(3-(5-fluoro-1,3-dioxo-1H-pyrido[1,2-c] pyrimidin-2(3H)-yl)-2-methylphenyl)-9H-carbazole-1-carboxamide (30); 4-(3-(5-chloro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(pyrrolidin-1-ylmethyl)-9H-carbazole-1-carboxamide (31); 4-(3-(5-fluoro-1,3-dioxo-1H-pyrido[1,2-c]pyrimidin-2(3H)-yl)-2-methylphenyl)-7-(methoxymethyl)-9H-carbazole-1-carboxamide (32); 8-fluoro-4-(3-(8-fluoro-1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)-2-methylphenyl)-7-(2-hydroxypropan-2-yl)-9H-carbazole-1-carboxamide (33); and salts thereof.

8. A pharmaceutical composition comprising a compound according to claim 1 or a pharmaceutically-acceptable salt thereof; and a pharmaceutically acceptable carrier.

9. A method of treating an autoimmune disease or a chronic inflammatory disease, comprising administering to a mammalian patient a compound according to claim 1 or a pharmaceutically acceptable salt thereof, wherein said autoimmune disease or chronic inflammatory disease is selected from multiple sclerosis, inflammatory bowel disease, and rheumatoid arthritis.