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Current Intelligence Bulletin 24: Direct Blue 6, Direct Black 38, and Direct Brown 95 Benzidine Derived Dyes										Related Pages
										April 17, 1978
DHHS (NIOSH) Publication Number 78-148

Identifiers and Synonyms for Direct Black 38
Chemical Abstracts Service Registry Number 1937-37-7
NIOSH/RTECS Number JM71700
Chemical Formula C34H25N9O7S2Na2
Identifiers and Synonyms for Direct Black 38
Ahco Direct Black GX
Airedale Black EDAizen Direct Deep Black EH
Aizen Direct Deep Black GHAizen Direct Deep Black RH
Amanil Black GLAmanil Black WD
Apomine Black GXAtlantic Black BD
Atlantic Black CAtlantic Black E
Atlantic Black EAAtlantic Black GAC
Atlantic Black GGAtlantic Black GXCW
Atlantic Black GXOOAtlantic Black SD
Atul Direct Black E8Azine Deep Black EW
Azocard Black EWAzomine Black EWO
Belamine Black GXBencidal Black E
Benzanil Black EBenzo Deep Black E
Benzo Leather Black EBenzoform Black BCN-CF
Black 2EMBLBlack 4EMBL
Brasilamina Black GNBrilliant Chrome Leather Black H
C.I. 30235C.I. Direct Black 38
Calcomine BlackCalcomine Black EXL
Carbide Black EChloramine Black C
Chloramine Black ECChloramine Black ERT
Chloramine Black EXChloramine Black EXR
Chloramine Black XOChloramine Carbon Black S
Chloramine Carbon Black SJChloramine Carbon Black SN
Chlorazol Black EChlorazol Black E (Biological Stain)
Chlorazol Black EAChlorazol Black EN
Chlorazol Burl Black EChlorazol Leather Black ENP
Chlorazol Silk Black GChrome Leather Black E
Chrome Leather Black ECChrome Leather Black EM
Chrome Leather Black GChrome Leather Brilliant Black ER
Coir Deep BlackColumbia Black EP C
Diacotton Deep BlackDiacotton Deep Black RX
Diamine Deep Black ECDiamine Direct Black E
Diaphtamine Black VDiazine Black E
Diazine Direct Black EDiazine Direct Black G
Diazol Black 2VDiphenyl Deep Black G
Direct Black MethylDirect Black A
Direct Black BRNDirect Black CX
Direct Black CXRDirect Black E
Direct Black EWDirect Black EX
Direct Black FRDirect Black GAC
Direct Black GWDirect Black GX
Direct Black GXRDirect Black JET
Direct Black MetaDirect Black Methyl
Direct Black NDirect Black RX
Direct Black SDDirect Black WS
Direct Black ZDirect Black 3
Direct Black 38Direct Deep Black E
Direct Deep Black E ExtraDirect Deep Black EA-CF
Direct Deep Black EACDirect Deep Black EW
Direct Deep Black EXEnianil Black CN
Erie Black BErie Black BF
Erie Black GACErie Black GAC
Erie Black GXOOErie Black JET
Erie Black NUGErie Black RXOO
Erie Brilliant Black SErie Fibre Black VP
Fenamin Black EFibre Black VF
Fixanol Black EFormaline Black C
Formic Black CFormic Black CW
Formic Black EAFormic Black MTG
Formic Black TGHispamin Black EF
Interchem Direct Black ZKayaku Direct Deep Black EX
Kayaku Direct Deep Black GXKayaku Direct Deep Black S
Kayaku Direct Leather Black EXKayaku Direct Special Black AAX
Lurazol Black BAMeta Black
Mitsui Direct Black EXMitsui Direct Black GX
Nippon Deep BlackNippon Deep Black GX
Paper Black BAPaper Black T
Paper Deep Black CParamine Black B
Paramine Black EPeeramine Black E
Peeramine Black GXOOPhenamine Black BCN-CF
Phenamine Black CLPhenamine Black E
Phenamine Black E 200Pheno Black EP
Pheno Black SGNPontamine Black E
Pontamine Black EBNSandopel Black EX
Seristan Black BTelon Fast Black E
Tetrazo Deep Black GTertrodirect Black E
Tetrodirect Black EFDUnion Black EM
Vondacel Black N
IDENTIFIERS AND SYNONYMS FOR DIRECT BLUE 6
Chemical Abstracts Service Registry Number 2602-46-2
NIOSH/RTECS Number QJ64000
Chemical Formula C32H20O14N6S4Na4
IDENTIFIERS AND SYNONYMS FOR DIRECT BLUE 6
Airedale Blue 2BD
Aizen Direct Blue 2BHAmanil Blue 2BX
Atlantic Blue 2BAtul Direct Blue 2B
Azocard Blue 2BAzomine Blue 2B
Belamine Blue 2BBencidal Blue 2B
Benzanil Blue 2BBenzo Blue BBA-CF
Benzo Blue BBN-CFBenzo Blue GS
Blue 2BBlue 2B Salt
Brasilamina Blue 2BCalcomine Blue 2B
Chloramine Blue 2BChlorazol Blue B
Chlorazol Blue BPChrome Leather Blue 2B
CI 22610C.I. 22610
C.I. Direct Blue 6C.I. Direct Blue 6, Tetrasodium Salt
Cresotine Blue 2BDiacotton Blue BB
Diamine Blue 2BDiamine Blue BB
Diaphtamine Blue BBDiazine Blue 2B
Diazol Blue 2BDiphenyl Blue 2B
Diphenyl Blue KFDiphenyl Blue M2B
Direct Blue ADirect Blue 2B
Direct Blue 6Direct Blue BB
Direct Blue GSDirect Blue K
Direct Blue M2BEnianil Blue 2BN
Fenamin Blue 2BFixanol Blue 2B
Hispamin Blue 2BIndigo Blue 2B
Kayaku DirectKayaku Direct Blue BB
Mitsui Direct Blue 2BNNaphtamine Blue 2B
Niagara Blue 2BNippon Blue BB
Paramine Blue 2BPhenamine Blue BB
Pheno Blue 2BPontamine Blue BB
Sodium Diphenyl-4,4′-Bix-Azo-2′′-8′′-Amino-
1′′-Naphthol-3′′,6′′-DisulphonateTertrodirect Blue 2B
Vondacel Blue 2B

IDENTFIERS AND SYNONYMS FOR DIRECT BROWN 95
Chemical Abstracts Service Registry Number 16071-86-6
NIOSH/RTECS Number JM78780
Chemical Formula C 31H 20N 6O 9SNa 2Cu

IDENTFIERS AND SYNONYMS FOR DIRECT BROWN 95
Aizen Primula Brown BRLH
Aizen Primula Brown PLHAmanil Fast Brown BRL
Amanil Supra Brown LBLAtlantic Fast Brown BRL
Atlantic Resin Fast Brown BRLBelamine Fast Brown BRLL
Benzanil Supra Brown BRLLBenzanil Supra Brown BRLN
Brown 4EMBLC.I. 30145
C.I. Direct BrownCalcodur Brown BRL
Chloramine Fast Brown BRLChloramine Fast Cutch Brown PL
Chlorantine Fast Brown BRLLChrome Leather Brown BRLL
Chrome Leather Brown BRSLCuprofix Brown GL
Derma Fast Brown W-GLDermafix Brown PL
Dialuminous Brown BRSDiaphtamine Light Brown BRLL
Diazine Fast Brown RSLDiazol Light Brown BRN
Dicorel Brown LMRDiphenyl Fast Brown BRL
Direct Brown 95Direct Brown BRL
Direct Fast Brown BRLDirect Fast Brown LMR
Direct Light Brown BRSDirect Supra Light Brown ML
Durazol Brown BRDurofast Brown BRL
Eliamina Light Brown BRLEnianil Light Brown BRL
Fastolite Brown BRLFastusol Brown LBRSA
Fastusol Brown LBRSNFenaluz Brown BRL
Helion Brown BRSLHispaluz Brown BRL
KCA Light Fast Brown BRKayarus Supra Brown BRS
Paranol Fast Brown BRLPeeramine Fast Brown BRL
Pontamine Fast Brown BRLPontamine Fast Brown NP
Pyrazol Fast Brown BRLPyrazoline Brown BRL
Saturn Brown LBRSirius Supra Brown BRL
Sirius Supra Brown BRSSolantine Brown BRL
Solar Brown PLSolex Brown R
Solius Light Brown BRLLSolius Light Brown BRS
Sumilight Supra Brown BRSSuprazo Brown BRL
Suprexcel Brown BRLTertrodirect Fast Brown BR
Tetramine Fast Brown BRDN ExtraTetramine Fast Brown BRP
Tetramine Fast Brown BRSTriantine Brown BRS
Triantine Fast Brown OGTriantine Fast Brown OR
Triantine Light Brown BRSTriantine Light Brown OG
NIOSH/NCI Joint Current Intelligence Bulletin
Direct Black 38, Direct Blue 6, and Direct Brown 95 Benzidine-Derived Dyes
The National Institute for Occupational Safety and Health (NIOSH) recommends that three widely used benzidine-derived dyes, Direct Black 38, Direct Blue 6, and Direct Brown 95, be handled in the workplace as if they were human carcinogens. This recommendation is based primarily upon a preliminary analysis of National Cancer Institute (NCI) data from short-term feeding studies, and on early results from NIOSH field studies. Cancerous and precancerous liver conditions were found in rats, similar to the damage produced by known liver carcinogens. Degeneration of liver cells was found in mice. Although the dyes tested by NCI contained less than 4 ppm residual benzidine when fed to the test animals, greater quantities of benzidine were found in the urine of dosed rats and mice. Caution is also indicated by preliminary results from NIOSH field studies showing that humans working with these same dyes also excrete higher than expected levels of benzidine in their urine. Both laboratory and field studies indicate that these benzidine-derived dyes can be metabolized to benzidine which is present in the urine of animals and humans.
Based on the data from the short-term study, NCI scientists believe a cancer causing potential exists upon exposure to the benzidine-derived dyes, most likely through the mechanism of metabolic conversion of the dyes to benzidine in the animal system. This NIOSH/NCI Joint Bulletin summarizes the results of the NCI animal study, the NIOSH field studies, other pertinent data, their implication for occupational health, and suggested guidelines for minimizing employee exposure to the three dyes.

Potential Occupational Exposures
The National Occupational Hazard Survey (NOHS), conducted between 1972 and 1974 by the National Institute for Occupational Safety and Health, indicates that workers are occupationally exposed to Direct Black 38, Direct Blue 6, and Direct Brown 95 in a variety of industries including: paper and allied products petroleum and related industries, rubber and plastic products, leather and leather products instrumentation and measuring devices, and banking. In addition, the textile industry accounts for a substantial occupational exposure. It is estimated that 25 percent of the benzidine-derived azo dyes are applied to textiles, 40 percent to paper, 15 percent to leather, and the remainder to other diverse applications.1
The Colour Index,2 a reference by Great Britain’s Society of Dyers and Colourists, reports the following uses for the three dyes:
Direct Black 38: Dyeing or staining of wool, silk, fibers for rope and matting, hogs hair, cellulose, acetate, nylon, and biological stains.
Direct Brown 95: Dyeing or staining silk, cotton, acetate, cellulose, wool, nylon, leather, paper, and certain plastics.
Direct Blue 6: Dyeing or staining silk, wool, cotton, nylon, leather, paper, biological stains and writing inks.Historically, benzidine has been an important intermediate in dye production since its introduction to the dyestuff industry around 1890.3,4 In 1948, production of benzidine-derived dyes was 35 million pounds which accounted for 25% of all domestic dyes manufactured and almost all of the direct class dyes.5 Domestic production of direct benzidine-derived dyes has dropped to 11.4 million pounds in 1971.6 Domestic production in 1978 should be limited to 12 benzidine dyes. The latest domestic production figures for Direct Black 38 show 7.3 million pounds in 1971,6 down to 2.2 million pounds in 1975,7 which rose to 3.76 million pounds in 1976.8 Direct Brown 95 has shown an increased production to 600,000 pounds in 1976 which is up from 406,000 pounds in 1975 and 343,000 pounds in 1974.8 Domestic production for Direct Blue 6 indicates that 327,000 pounds were produced in 1973, which is the last available figure for that dye.9
In the general population, unspecified exposure levels to the three dyes are thought to occur through the use of retail packaged dyes for home dyeing and for home and school use in art and craft projects such as tie-dyeing or batik. The Art Hazards Project of the Center for Occupational Hazards, New York City, has reported that package dyes sold in supermarkets, variety stores and hardware stores are combinations of direct, acid and basic dyes, and thus may contain benzidine-derived dye components.10 Two of these dyes, Direct Black 38 and Direct Blue 6, have been used in hair dyes.11

NCI Dose-Ranging Feeding Study11
Ninety-day animal feeding tests have been completed by the National Cancer Institute for three widely-used dyes, Direct Black 38, Direct Blue 6, and Direct Brown 95. The first-phase tests are conducted routinely to establish dosage levels in mice and rats for chemicals being screened for cancer-causing activity. The dose-ranging studies commonly precede standard bioassays–animal lifetime tests at dosages that do not shorten lifespans or impair growth.
All three of the dyes are benzidine-derived, having a unit of benzidine in their chemical structure. Benzidine is a known animal and human cancer-causing agent.12 Residual free benzidine in the feed was below 4 parts per million (ppm).
A total of 120 rats and 120 mice were divided into groups of 10. One group of each sex and each species was reserved as undosed controls, while five groups of each sex and species received differing dosages of the dyes in feed. At the 4th and 12th week of the study, urine was collected from dosed rats and mice for benzidine analysis. After 91 days of feeding and one day of observation, the surviving animals were killed and their tissues examined.
After the 90-day trial, significant incidences of cancerous and precancerous changes in the liver were found in both male and female rats dosed with any of the three dyes, whereas, untreated control rats had no liver damage. The liver changes in dosed animals were similar to changes caused by benzidine.
In mice, all three dyes were found harmful to the liver, but no cancerous changes were found. This finding is compatible with the interpretation that the toxic effects may be related to benzidine, because benzidine is more likely to produce cancer in rats than in mice. No liver abnormalities or damage to any internal organs were found in the control animals of either species.
In addition, although the dyes were essentially benzidine-free when fed to the animals, substantial benzidine was found in the urine of dosed rats and mice, an indication that animal systems metabolize the dyes to benzidine.
The technical report *“13-Week Subchronic Toxicity Studies — Direct Blue 6, Direct Black 38, and Direct Brown 95 Dyes”* is available from the Office of Cancer Communications, National Cancer Institute, Bethesda, Maryland 20014.

Other Laboratory Animal Studies
As an historical point, an early realization of the metabolism of azo compounds in mammals came as a result of feeding Orange I, an azo dye, to dogs in 1911.13 An intermediate of the dye, sulfanilic acid, was identified in the urine demonstrating for the first time that azo compounds may be metabolized by reductive cleavage of the azo group. Since then it has been repeatedly demonstrated that the intestinal flora within animals and many animal hepatic enzyme systems are capable of splitting the azo bond.14,15 The majority of this work was performed with azo dyes intended for food coloring.
Yoshida and Miyakawa found that when sulfonated benzidine azo dyes were injected into surgically removed mice intestines and then incubated, free benzidine was later found. They also showed that Escherichia coli as well as soil bacteria were quite capable of reducing benzidine dyes when incubated at 37 C.16
Another study showed that rhesus monkeys, fed benzidine-derived dyes with no residual benzidine, excreted benzidine in their urine. The levels excreted were estimated as being almost as high as if an equal amount of pure benzidine, as found in the dye moiety, were fed instead to the monkeys.17 These studies lead NIOSH to believe that when dyes of these types are ingested by man, they result in benzidine in the urine thus posing a potential carcinogenic hazard.

NIOSH Field Studies
Preliminary results from NIOSH studies indicate the presence of benzidine, or monacetylbenzidine (MAB), a metabolite of benzidine, in the urine of workers in four out of five industrial facilities in which urine samples were collected. The facilities surveyed to date include two benzidine dye manufacturers, two textile finishing companies and a leather tannery.
In one dye manufacturing plant, benzidine and MAB were found in all dye workers who worked solely with the finished dyes. Bulk benzidine-based dyes were quantitatively analyzed for residual free benzidine content which ranged from less than 1 to 19 ppm. It was conservatively estimated that about 20 times more benzidine and up to 200 times more MAB were present in the urine of these dye workers, than if they had been exposed only to the residual benzidine content in the dyes. The concentrations of benzidine found in the urine of these workers were significant fractions of those concentrations associated with a high incidence of bladder cancer as reported in the scientific literature. Among textile dyers included in the NIOSH study, four of ten had benzidine or MAB present in their urine and all had elevated aromatic amine levels. No benzidine or MAB was detected in urine samples collected at a leather tannery where good work practices were observed. While there was biological variability in body burden tolerance and differences in metabolism among those studied, it appears likely that work practices and personal hygiene played a major role in minimizing exposure. Analyses of data from these NIOSH studies are ongoing.
The relatively large particle size of the dry dye powders causes inhaled dyes to be deposited largely in the upper respiratory tract and then ingested. Prevention of worker inhalation or ingestion of these dyes would greatly reduce absorption into the body and subsequent benzidine exposure.

Epidemiological and Medical Studies — Benzidine-Based Dyes
A strong association relating human exposure to benzidine-based dyes with the subsequent development of bladder tumors was presented after a case-control mortality study of 200 bladder cancer patients in Japan.16 The patients were found to have been predominantly kimono painters and dyers. The kimono painters had the habit of forming a point on their brushes by drawing the brush between their lips, which allowed for ingestion of the dyes.
Several other case-control mortality studies indicate an increased risk of developing bladder cancer in the textile and leather industries, both large users of direct dyes. However, only a few references have been made concerning benzidine-derived dyestuffs.
In Russia, a medical study concerning the early detection of bladder tumors among textile dyers using benzidine-derived dyes found an unusual incidence of bladder lesions, some of which were suggested as being of a precancerous nature. The greatest number of such lesions were found in those workers with the highest potential exposure to these dyes.18
The National Institute for Occupational Safety and Health and the National Cancer Institute have jointly prepared this Bulletin to facilitate the rapid dissemination of our preliminary findings on three benzidine-derived dyes: Direct Black 38, Direct Blue 6, and Direct Brown 95. NIOSH recommends that these dyes be handled in the workplace as if they were human carcinogens and requests that producers, distributors, professional associations, and unions transmit the information in this Bulletin to their customers employees associates, and members.
[*signature*]
Arthur C. Upton, M.D.
Director
National Cancer Institute
[*signature*]
J. Donald Millar, M.D.
Assistant Surgeon General
Acting Director,
National Institute for Occupational
Safety and Health

NIOSH Action on Benzidine-Derived Dyes
The NIOSH Industrial Hygiene Section will shortly conduct a study in which workers who have had a one-day exposure to benzidine-based dyes are monitored over several days. This will hopefully confirm some assumptions on the excretion kinetics of such exposure and better establish the best time to monitor a worker’s urine for benzidine.
The NIOSH Clinical and Biochemical Support Section has an interagency agreement with the National Center for Toxicological Research to conduct animal metabolism studies. Test animals will be fed Direct Black 38, Direct Blue 6 and Direct Brown 95. Metabolites will be identified. in the urine and evaluated for mutagenic activity. Additional dyes made from 3,3-dimethyl- benzidine, 3,3-dimethoxybenzidine, and 3,3-dichlorobenzidine will also be tested.
Suggested Guidelines for Minimizing Employee Exposure to Direct Black 38, Direct Blue 6 Direct Brown 95 — Benzidine-Derived Dyes
NIOSH recommends that it would be prudent to handle Direct Black 38, Direct Blue 6, and Direct Brown 95 in the workplace as if they were human carcinogens. Exposure to Direct Black 38, Direct Blue 6, and Direct Brown 95 should be limited to as few employees as possible, while minimizing workplace exposure levels. The area in which they are used should be restricted to only those employees essential to the process or operation.

Exposure Monitoring
Initial and routine employee exposure surveys should be made by competent industrial hygiene and engineering personnel. These surveys are necessary to determine the extent of employee exposure and to ensure that controls are effective.
The *NIOSH Occupational Exposure Sampling Strategy Manual*, NIOSH Publication #77-173, may be helpful in developing efficient programs to monitor employee exposures to Direct Black 38, Direct Blue 6, and Direct Brown 95. The manual discusses determination of the need for exposure measurements, selection of appropriate employees for exposure evaluation, and selection of sampling times.
Employee exposure measurements should consist of 8-hour TWA (time-weighted average) exposure estimates calculated from personal or breathing zone samples (air that would most nearly represent that inhaled by the employees). Area and source measurements may be useful to determine problem areas, processes, and operations.

Minimizing Employee Exposure
There are four basic methods of limiting employee exposure to Direct Black 38, Direct Blue 6, and Direct Brown 95. None of these is a simple industrial hygiene or management decision and careful planning and thought should be used prior to implementation of any of these.
Product Substitution
The substitution of an alternative material with a lower potential health and safety risk is one method. However, extreme care must be used when selecting possible substitutes. Alternatives to Direct Black 38, Direct Blue 6, and Direct Brown 95 should be fully evaluated with regard to possible human effects. Unless the toxic effects of the alternative have been thoroughly evaluated, a seemingly safe replacement, possibly only after years of use, may be found to induce serious health effects.
Contaminant Controls
The most effective control of Direct Black 38, Direct Blue 6, and Direct Brown 95, where feasible, is at the source of contamination by enclosure of the operation and/or local exhaust ventilation.
If feasible, the process or operation should be enclosed with a slight vacuum so that any leakage will result in the flow of air into the enclosure.
The next most effective means of control would be a well designed local exhaust ventilation system that physically encloses the process as much as possible, with sufficient capture velocity to keep the contaminant from entering the work atmosphere.
To ensure that ventilation equipment is working properly effectiveness (e.g., air velocity, static pressure, or air volume) should be checked at least every three months. System effectiveness should be checked soon after any change in production, process, or control which might result in significant increases in airborne exposures to Direct Black 38, Direct Blue 6, and Direct Brown 95.
Employee Isolation
A third alternative is the isolation of employees. It frequently involves the use of automated equipment operated by personnel observing from a closed control booth or room. The control room is maintained at a greater air pressure than that surrounding the process equipment so that air flow is out of, rather than into, the room. This type of control will not protect those employees that must do process checks, adjustments, maintenance and related operations.
Personal Protective Equipment
The least preferred method is the use of personal protective equipment. This equipment, which may include respirators, goggles, gloves, and other devices should not be used as the only means to prevent or minimize exposure during routine operations.
Exposure to Direct Black 38, Direct Blue 6, and Direct Brown 95 should not be controlled with the use of respirators except:
During the time period necessary to install or implement engineering or work practice controls; or
In work situations in which engineering and work practice controls are technically not feasible; or
For maintenance; or
For operations which require entry into tanks or closed vessels; or
In emergencies.Only respirators approved by the National Institute for Occupational Safety and Health (NIOSH) should be used. Refer to *NIOSH Certified Equipment, December 15, 1975,* NIOSH publication #76-145 and *Cumulative Supplement June 1977, NIOSH Certified Equipment* NIOSH publication #77-195. The use of faceseal coverlets or socks with any respirator voids NIOSH approvals.
Quantitative faceseal fit test equipment (such as sodium chloride, dioctyl phthalate, or equivalent) should be used. Refer to *A guide to Industrial Respiratory Protection,* NIOSH publication #76-189 for guidelines on appropriate Respiratory protection programs.
In addition, proper maintenance procedures, good housekeeping in the work area and education of employees concerning the nature of the hazard, its control and personal hygiene are all aspects of a good control program.

References
Environmental Protection Agency, 40 CFR Part 129 “Benzidine: Proposed Toxic Pollutant Effluent Standards” Federal Register Vol. 41, No. 127, June 30, 1973.
Color Index, 1971, 3rd Ed. The Society of Dyers and Colourists -American Association of Textile Chemists and Colorists, Lund, Humphries, Bradford and London, (eds.), London, England.
Venkataraman, K., 1952. The Chemistry of Synthetic Dyes. Academic Press, New York p. 506-514.
Lubs, H.A., 1952. The Chemistry of Synthetic Dyes and Pigments. American Chemical Society Monograph No. 127,Washington, D.C.
Synthetic Organic Chemicals, 1948. U.S. Tariff Commission, Report on Synthetic Organic Dyes. Series 6-2.
U.S. International Trade Commission, United States Production & Sales, 1971. Synthetic Organic Chemicals (1973), Washington, D.C.
Synthetic Organic Chemicals: United States Production and Sales, 1975. U.S. International Trade Commission Report 806 (1977), Washington, D.C.
Synthetic Organic Chemicals: United States Production and Sales, 1974. U.S. International Trade Commission Report 762 (1976), Washington, D.C.
Synthetic Organic Chemicals: United States Production and Sales, 1973. U.S. International Trade Commission Report, Washington, D.C.
Jenkins, C. Dye Hazards Report, February, 1978.
13-Week Subchronic Toxicity Studies of Direct Blue 6, Direct Black 38, and Direct Brown 95 Dyes. National Cancer Institute. Carcinogenesis Technical Report. DHEW Publication #”NIH” 78-1358, 1978.
IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals to Man. Vol. 1, 80.
Sisley, P., Porcher, C. DuSort des Matieres Colorantes dans L’organesma animal. In Walker, R., 1970. The Metabolism of Azo Compounds – A Review of the Literature. Food and Cosmetic Toxicology, 8, 659-676.
Radomski, J., Hellinger, T., 1961. The Absorption, Rate, and Excretion in Rats of the Water Soluble Azo Dyes, FD&C Red No. 2, FD&C Red No. 4, and FD&C Yellow No. 6. J. Pharmaceutical and Experimental Therapy, 136, 259-266.
Roxan, J.J., Ryan, A.J., Wright, 1967. Reduction of Water Soluble Azo Dyes by Intestinal Bacteria. Food and Cosmetic Toxicology. S, 367-369.
Voshida, O., Miyakaqa, M., 1973. Etiology of Bladder Cancer: “Metabolic” Aspects. In Analytical and Experimental Epidemiology of Cancer. Nakahara, Hirayoma, Wishioka, et al., (eds.), University Park Press, Baltimore, P. 31-39.
Rinde, E., Troll, W., 1975. Metabolic Reduction of Benzidine Azo Dyes to Benzidine in the Rhesus Monkey. . National Cancer Institute, 55, 181-182.
Korosteleva, T.A., Kljuckareu, B.V., Belokhuostave, A.T., Ayzomyatnikov, A.A., 1973. Immunological Diagnosis in Early Stages of Occupational Cancer of the Urinary Bladder. Problems of Oncology 19.2, p. 2832.
							
					
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