# L_2007051EN.01000701.xml

| 20.2.2007 | EN | Official Journal of the European Union | L 51/7 |
| --- | --- | --- | --- |

20.2.2007

EN

Official Journal of the European Union

L 51/7

---

COMMISSION REGULATION (EC) No 162/2007

of 19 February 2007

amending Regulation (EC) No 2003/2003 of the European Parliament and of the Council relating to fertilisers for the purposes of adapting Annexes I and IV thereto to technical progress

(Text with EEA relevance)

THE COMMISSION OF THE EUROPEAN COMMUNITIES,

Having regard to the Treaty establishing the European Community,

Having regard to Regulation (EC) No 2003/2003 of the European Parliament and of the Council of 13 October 2003 relating to fertilisers (1), and in particular Article 31(3) thereof,

Whereas:

| (1) | Section E of Annex I to Regulation (EC) No 2003/2003 lists those types of inorganic micronutrient fertilisers which may be designated ‘EC fertiliser’ in accordance with Article 3 of that Regulation. The list includes a number of fertilisers in which the micronutrient is chemically combined with a chelating agent. A list of authorised chelating agents is provided in table E.3.1 of that Annex. |
| --- | --- |

(1)

Section E of Annex I to Regulation (EC) No 2003/2003 lists those types of inorganic micronutrient fertilisers which may be designated ‘EC fertiliser’ in accordance with Article 3 of that Regulation. The list includes a number of fertilisers in which the micronutrient is chemically combined with a chelating agent. A list of authorised chelating agents is provided in table E.3.1 of that Annex.

| (2) | The specification of the fertiliser type in which the chelated micronutrient is iron allows the use of either a single authorised chelating agent, or of a mixture of them, provided that chelated fraction can be quantified by the method described in European Standard EN 13366, and the individual chelating agents in the mixture can be separately identified and quantified by EN 13368. |
| --- | --- |

(2)

The specification of the fertiliser type in which the chelated micronutrient is iron allows the use of either a single authorised chelating agent, or of a mixture of them, provided that chelated fraction can be quantified by the method described in European Standard EN 13366, and the individual chelating agents in the mixture can be separately identified and quantified by EN 13368.

| (3) | The provisions for iron micronutrient fertiliser containing chelated iron should be updated in three respects. First, to make clear that at least 50 % of the water-soluble iron must be chelated by the authorised chelating agents. Second, to specify that an authorised chelating agent can be mentioned on the fertiliser type designation only if it chelates at least 1 % of the water-soluble iron. Third, to generalise the reference to European Standards in order to allow the use of additional European Standards. |
| --- | --- |

(3)

The provisions for iron micronutrient fertiliser containing chelated iron should be updated in three respects. First, to make clear that at least 50 % of the water-soluble iron must be chelated by the authorised chelating agents. Second, to specify that an authorised chelating agent can be mentioned on the fertiliser type designation only if it chelates at least 1 % of the water-soluble iron. Third, to generalise the reference to European Standards in order to allow the use of additional European Standards.

| (4) | The chemical names of the authorised chelating agents listed in section E.3.1 of Annex I to Regulation (EC) No 2003/2003 are intended to distinguish between different isomers of the same substance in a descriptive way. As there are several different nomenclatures in common use in the scientific community for those substances, there is a risk of misidentification. In order to ensure unambiguous identification of the chelating agents, the corresponding CAS numbers (Chemical Abstracts Service of the American Chemical Society), which uniquely identify the different isomers of the chelating agents, should be provided for each entry of that Annex. It is therefore appropriate to delete three chelating agents isomers which cannot be unambiguously identified by a CAS number. |
| --- | --- |

(4)

The chemical names of the authorised chelating agents listed in section E.3.1 of Annex I to Regulation (EC) No 2003/2003 are intended to distinguish between different isomers of the same substance in a descriptive way. As there are several different nomenclatures in common use in the scientific community for those substances, there is a risk of misidentification. In order to ensure unambiguous identification of the chelating agents, the corresponding CAS numbers (Chemical Abstracts Service of the American Chemical Society), which uniquely identify the different isomers of the chelating agents, should be provided for each entry of that Annex. It is therefore appropriate to delete three chelating agents isomers which cannot be unambiguously identified by a CAS number.

| (5) | A more consistent nomenclature should be used for the chelating agents, and the statement according to which authorised chelating agents must also comply with other Community legislation should be made more precise. |
| --- | --- |

(5)

A more consistent nomenclature should be used for the chelating agents, and the statement according to which authorised chelating agents must also comply with other Community legislation should be made more precise.

| (6) | Annex IV to Regulation (EC) No 2003/2003 provides detailed descriptions of the methods of analysis to be used to measure the nutrient content of EC fertilisers. Those descriptions need to be adjusted in order to have correct analysis values. |
| --- | --- |

(6)

Annex IV to Regulation (EC) No 2003/2003 provides detailed descriptions of the methods of analysis to be used to measure the nutrient content of EC fertilisers. Those descriptions need to be adjusted in order to have correct analysis values.

| (7) | Regulation (EC) No 2003/2003 should therefore be amended accordingly. |
| --- | --- |

(7)

Regulation (EC) No 2003/2003 should therefore be amended accordingly.

| (8) | The measures provided for in this Regulation are in accordance with the opinion of the Committee established by Article 32 of Regulation (EC) No 2003/2003, |
| --- | --- |

(8)

The measures provided for in this Regulation are in accordance with the opinion of the Committee established by Article 32 of Regulation (EC) No 2003/2003,

HAS ADOPTED THIS REGULATION:

Article 1

1.   Annex I to Regulation (EC) No 2003/2003 is amended in accordance with Annex I to this Regulation.

2.   Annex IV to Regulation (EC) No 2003/2003 is amended in accordance with Annex II to this Regulation.

Article 2

This Regulation shall enter into force on the 20th day following that of its publication in the Official Journal of the European Union.

This Regulation shall be binding in its entirety and directly applicable in all Member States.

Done at Brussels, 19 February 2007.

For the Commission

Günter VERHEUGEN

Vice-President

---

(1)  
  OJ L 304, 21.11.2003, p. 1. Regulation as last amended by Council Regulation (EC) No 1791/2006 (OJ L 363, 20.12.2006, p. 1).

1

---

ANNEX I

Annex I to Regulation (EC) No 2003/2003 is amended as follows:

| 1. | Table E.1.4 is replaced by the following table:
  ‘E.1.4.   Iron










  No | Type designation | Data on method of production and essential ingredients | Minimum content of nutrients
  (percentage by weight)
  Data on the expression of nutrients
  Other requirements | Other data on the type of designation | Nutrient content to be declared
  Forms and solubilities of the nutrients
  Other criteria |
| --- | --- | --- | --- | --- | --- | --- |
| 1 | 2 | 3 | 4 | 5 | 6 |
| 4a | Iron salt | Chemically obtained product containing a mineral iron salt as its essential ingredient | 12 % water-soluble Fe | The designation must include the name of the mineral anion | Water-soluble iron (Fe) |
| 4b | Iron chelate | Water-soluble product obtained by chemical reaction of iron with chelating agent(s) mentioned in the list of Annex I section E.3. | 5 % of water-soluble iron, of which the chelated fraction is at least 80 %, and at least 50 % of the water-soluble iron is chelated by the declared chelating agent(s) | Name of each one of the chelating agents mentioned in the list of Annex I section E.3.1 that chelates at least 1 % water-soluble iron | Water-soluble iron (Fe)
  Iron (Fe) chelated by each chelating agent that is declared in the type-designation and that can be identified and quantified by a European Standard |
| 4c | Iron fertiliser solution | Product obtained by dissolving types 4a and/or one of the type 4b in water | 2 % of water soluble Fe | The designation must include:






  1. | the name(s) of the mineral anion(s); |

1.

Table E.1.4 is replaced by the following table:

‘E.1.4.   Iron

No

Type designation

Data on method of production and essential ingredients

Minimum content of nutrients

(percentage by weight)

Data on the expression of nutrients

Other requirements

Other data on the type of designation

Nutrient content to be declared

Forms and solubilities of the nutrients

Other criteria

1

2

3

4

5

6

4a

Iron salt

Chemically obtained product containing a mineral iron salt as its essential ingredient

12 % water-soluble Fe

The designation must include the name of the mineral anion

Water-soluble iron (Fe)

4b

Iron chelate

Water-soluble product obtained by chemical reaction of iron with chelating agent(s) mentioned in the list of Annex I section E.3.

5 % of water-soluble iron, of which the chelated fraction is at least 80 %, and at least 50 % of the water-soluble iron is chelated by the declared chelating agent(s)

Name of each one of the chelating agents mentioned in the list of Annex I section E.3.1 that chelates at least 1 % water-soluble iron

Water-soluble iron (Fe)

Iron (Fe) chelated by each chelating agent that is declared in the type-designation and that can be identified and quantified by a European Standard

4c

Iron fertiliser solution

Product obtained by dissolving types 4a and/or one of the type 4b in water

2 % of water soluble Fe

The designation must include:

1.

the name(s) of the mineral anion(s);

| 2. | the name of any chelating agent, if present, that chelates at least 1 % water-soluble iron (Fe) |
| --- | --- |

2.

the name of any chelating agent, if present, that chelates at least 1 % water-soluble iron (Fe)

Water-soluble iron (Fe)

Chelated iron (Fe) if present

Iron (Fe) chelated by each chelating agent that is declared in the type-designation and that can be identified and quantified by a European Standard’

| 2. | Section E.3 is replaced by the following:
  ‘E.3.   List of authorised organic chelating and complexing agents for micro-nutrients
  The following substances are authorised provided that their corresponding nutrient chelate has complied with the requirements of Council Directive 67/548/EEC (*1).
  E.3.1.   Chelating agents (*2)

  Acids, or sodium, potassium or ammonium salts of: | CAS number of the acid (*3) |
| --- | --- | --- |
| Ethylenediaminetetraacetic acid | EDTA | C10H16O8N2 | 60-00-4 |
| 2-hydroxyethylethylenediaminetriacetic acid | HEEDTA | C10H18O7N2 | 150-39-0 |
| diethylenetriaminepentaacetic acid | DTPA | C14H23O10N3 | 67-43-6 |
| ethylenediamine-N,N′-di[(ortho-hydroxyphenyl)acetic acid] | [o,o] EDDHA | C18H20O6N2 | 1170-02-1 |
| ethylenediamine-N-[(ortho-hydroxyphenyl)acetic acid]-N′-[(para-hydroxyphenyl)acetic acid] | [o,p] EDDHA | C18H20O6N2 | 475475-49-1 |
| ethylenediamine-N,N′-di[(ortho-hydroxy-methylphenyl)acetic acid] | [o,o] EDDHMA | C20H24O6N2 | 641632-90-8 |
| ethylenediamine-N-[(ortho-hydroxy-methylphenyl)acetic acid]-N′-[(para-hydroxy-methylphenyl)acetic acid] | [o,p] EDDHMA | C20H24O6N2 | 641633-41-2 |
| ethylenediamine-N,N′-di[(5-carboxy-2-hydroxyphenyl)acetic acid] | EDDCHA | C20H20O10N2 | 85120-53-2 |
| ethylenediamine-N,N′-di[(2-hydroxy-5-sulfophenyl)acetic acid] and its condensation products | EDDHSA | C18H20O12N2S2 + n*(C12H14O8N2S) | 57368-07-7 and 642045-40-7 |

2.

Section E.3 is replaced by the following:

‘E.3.   List of authorised organic chelating and complexing agents for micro-nutrients

The following substances are authorised provided that their corresponding nutrient chelate has complied with the requirements of Council Directive 67/548/EEC (*1).

E.3.1.   Chelating agents (*2)

Acids, or sodium, potassium or ammonium salts of:

CAS number of the acid (*3)

Ethylenediaminetetraacetic acid

EDTA

C10H16O8N2

60-00-4

2-hydroxyethylethylenediaminetriacetic acid

HEEDTA

C10H18O7N2

150-39-0

diethylenetriaminepentaacetic acid

DTPA

C14H23O10N3

67-43-6

ethylenediamine-N,N′-di[(ortho-hydroxyphenyl)acetic acid]

[o,o] EDDHA

C18H20O6N2

1170-02-1

ethylenediamine-N-[(ortho-hydroxyphenyl)acetic acid]-N′-[(para-hydroxyphenyl)acetic acid]

[o,p] EDDHA

C18H20O6N2

475475-49-1

ethylenediamine-N,N′-di[(ortho-hydroxy-methylphenyl)acetic acid]

[o,o] EDDHMA

C20H24O6N2

641632-90-8

ethylenediamine-N-[(ortho-hydroxy-methylphenyl)acetic acid]-N′-[(para-hydroxy-methylphenyl)acetic acid]

[o,p] EDDHMA

C20H24O6N2

641633-41-2

ethylenediamine-N,N′-di[(5-carboxy-2-hydroxyphenyl)acetic acid]

EDDCHA

C20H20O10N2

85120-53-2

ethylenediamine-N,N′-di[(2-hydroxy-5-sulfophenyl)acetic acid] and its condensation products

EDDHSA

C18H20O12N2S2 + n*(C12H14O8N2S)

57368-07-7 and 642045-40-7

E.3.2.   Complexing agents:

List to be drawn up.

(*1)  
  OJ 196, 16.8.1967, p. 1."

(*2)  The chelating agents are to be identified and quantified by the European Standards that covers the mentioned chelating agents."

---

(*1)  
  OJ 196, 16.8.1967, p. 1.

(*2)  The chelating agents are to be identified and quantified by the European Standards that covers the mentioned chelating agents.

---

ANNEX II

Annex IV, B, to Regulation (EC) No 2003/2003 is amended as follows:

| 1. | Method 2 is amended as follows:






  (a) | Method 2.1 is amended as follows:






  (i) | points 4.2 to 4.7 are replaced by the following:












  ‘4.2. | Sulphuric acid: 0,05 mol/l |
| --- | --- | --- | --- | --- |

1.

Method 2 is amended as follows:

(a)

Method 2.1 is amended as follows:

(i)

points 4.2 to 4.7 are replaced by the following:

‘4.2.

Sulphuric acid: 0,05 mol/l

for variant a.

| 4.3. | Sodium or potassium hydroxide solution, carbonate free: 0,1 mol/l |
| --- | --- |

4.3.

Sodium or potassium hydroxide solution, carbonate free: 0,1 mol/l

| 4.4. | Sulphuric acid: 0,1 mol/l |
| --- | --- |

4.4.

Sulphuric acid: 0,1 mol/l

for variant b (see note 2).

| 4.5. | Sodium or potassium hydroxide solution, carbonate free: 0,2 mol/l |
| --- | --- |

4.5.

Sodium or potassium hydroxide solution, carbonate free: 0,2 mol/l

| 4.6. | Sulphuric acid: 0,25 mol/l |
| --- | --- |

4.6.

Sulphuric acid: 0,25 mol/l

for variant c (see note 2).’

| 4.7. | Sodium or potassium hydroxide solution, carbonate free: 0,5 mol/l |
| --- | --- |

4.7.

Sodium or potassium hydroxide solution, carbonate free: 0,5 mol/l

| (ii) | in point 9, table 1, variant a, the second sentence is replaced by the following:

  ‘Sulphuric acid 0,05 mol/l to be placed in the receiving flask: 50 ml’; |
| --- | --- |

(ii)

in point 9, table 1, variant a, the second sentence is replaced by the following:

‘Sulphuric acid 0,05 mol/l to be placed in the receiving flask: 50 ml’;

| (iii) | in point 9, table 1, variant b, the second sentence is replaced by the following:

  ‘Sulphuric acid 0,1 mol/l to be placed in the receiving flask: 50 ml’; |
| --- | --- |

(iii)

in point 9, table 1, variant b, the second sentence is replaced by the following:

‘Sulphuric acid 0,1 mol/l to be placed in the receiving flask: 50 ml’;

| (iv) | in point 9, table 1, variant c, the second sentence is replaced by the following:

  ‘Sulphuric acid 0,25 mol/l to be placed in the receiving flask: 35 ml’; |
| --- | --- |

(iv)

in point 9, table 1, variant c, the second sentence is replaced by the following:

‘Sulphuric acid 0,25 mol/l to be placed in the receiving flask: 35 ml’;

| (b) | in Method 2.2.1, point 4.2 is replaced by the following:






  ‘4.2. | Sulphuric acid 0,05 mol/l’; |
| --- | --- | --- |

(b)

in Method 2.2.1, point 4.2 is replaced by the following:

‘4.2.

| (c) | Method 2.2.2 is amended as follows:






  (i) | points 4.2 to 4.7 are replaced by the following:












  ‘4.2. | Sulphuric acid: 0,05 mol/l |
| --- | --- | --- | --- |

(c)

Method 2.2.2 is amended as follows:

(i)

points 4.2 to 4.7 are replaced by the following:

‘4.2.

Sulphuric acid: 0,05 mol/l

for variant a.

| 4.3. | Sodium or potassium hydroxide solution, carbonate free: 0,1 mol/l |
| --- | --- |

4.3.

Sodium or potassium hydroxide solution, carbonate free: 0,1 mol/l

| 4.4. | Sulphuric acid: 0,1 mol/l |
| --- | --- |

4.4.

Sulphuric acid: 0,1 mol/l

for variant b (see note 2, Method 2.1).

| 4.5. | Sodium or potassium hydroxide solution, carbonate free: 0,2 mol/l |
| --- | --- |

4.5.

Sodium or potassium hydroxide solution, carbonate free: 0,2 mol/l

| 4.6. | Sulphuric acid: 0,25 mol/l |
| --- | --- |

4.6.

Sulphuric acid: 0,25 mol/l

for variant c (see note 2, Method 2.1).’

| 4.7. | Sodium or potassium hydroxide solution, carbonate free: 0,5 mol/l |
| --- | --- |

4.7.

Sodium or potassium hydroxide solution, carbonate free: 0,5 mol/l

| (ii) | point 7.4 is replaced by the following:
  ‘7.4.   Control test
  Before analysing, check that the apparatus is working properly and that the correct technique is applied using an aliquot part of a freshly prepared solution of sodium nitrate (4.13) containing 0,050 to 0,150 g of nitrate nitrogen depending on the variant chosen.’; |
| --- | --- |

(ii)

point 7.4 is replaced by the following:

‘7.4.   Control test

Before analysing, check that the apparatus is working properly and that the correct technique is applied using an aliquot part of a freshly prepared solution of sodium nitrate (4.13) containing 0,050 to 0,150 g of nitrate nitrogen depending on the variant chosen.’;

| (d) | in Method 2.2.3, points 4.2 to 4.7 are replaced by the following:












  ‘4.2. | Sulphuric acid: 0,05 mol/l |
| --- | --- | --- |

(d)

in Method 2.2.3, points 4.2 to 4.7 are replaced by the following:

‘4.2.

Sulphuric acid: 0,05 mol/l

for variant a.

| 4.3. | Sodium or potassium hydroxide solution, carbonate free: 0,1 mol/l |
| --- | --- |

4.3.

Sodium or potassium hydroxide solution, carbonate free: 0,1 mol/l

| 4.4. | Sulphuric acid: 0,1 mol/l |
| --- | --- |

4.4.

Sulphuric acid: 0,1 mol/l

for variant b (see note 2, Method 2.1).

| 4.5. | Sodium or potassium hydroxide solution, carbonate free: 0,2 mol/l |
| --- | --- |

4.5.

Sodium or potassium hydroxide solution, carbonate free: 0,2 mol/l

| 4.6. | Sulphuric acid: 0,25 mol/l |
| --- | --- |

4.6.

Sulphuric acid: 0,25 mol/l

for variant c (see note 2, Method 2.1).’

| 4.7. | Sodium or potassium hydroxide solution, carbonate free: 0,5 mol/l |
| --- | --- |

4.7.

Sodium or potassium hydroxide solution, carbonate free: 0,5 mol/l

| (e) | in Method 2.3.1, points 4.5 to 4.10 are replaced by the following:












  ‘4.5. | Sulphuric acid: 0,05 mol/l |
| --- | --- | --- |

(e)

in Method 2.3.1, points 4.5 to 4.10 are replaced by the following:

‘4.5.

Sulphuric acid: 0,05 mol/l

for variant a (see Method 2.1).

| 4.6. | Sodium or potassium hydroxide solution, carbonate free: 0,1 mol/l |
| --- | --- |

4.6.

Sodium or potassium hydroxide solution, carbonate free: 0,1 mol/l

| 4.7. | Sulphuric acid: 0,1 mol/l |
| --- | --- |

4.7.

Sulphuric acid: 0,1 mol/l

for variant b (see note 2, Method 2.1).

| 4.8. | Sodium or potassium hydroxide solution, carbonate free: 0,2 mol/l |
| --- | --- |

4.8.

Sodium or potassium hydroxide solution, carbonate free: 0,2 mol/l

| 4.9. | Sulphuric acid: 0,25 mol/l |
| --- | --- |

4.9.

Sulphuric acid: 0,25 mol/l

for variant c (see note 2, Method 2.1).’

| 4.10. | Sodium or potassium hydroxide solution, carbonate free: 0,5 mol/l |
| --- | --- |

4.10.

Sodium or potassium hydroxide solution, carbonate free: 0,5 mol/l

| (f) | in Method 2.3.2, points 4.4 to 4.9 are replaced by the following:












  ‘4.4. | Sulphuric acid: 0,05 mol/l |
| --- | --- | --- |

(f)

in Method 2.3.2, points 4.4 to 4.9 are replaced by the following:

‘4.4.

Sulphuric acid: 0,05 mol/l

for variant a (see Method 2.1).

| 4.5. | Sodium or potassium hydroxide solution, carbonate free: 0,1 mol/l |
| --- | --- |

4.5.

Sodium or potassium hydroxide solution, carbonate free: 0,1 mol/l

| 4.6. | Sulphuric acid: 0,1 mol/l |
| --- | --- |

4.6.

Sulphuric acid: 0,1 mol/l

for variant b (see note 2, Method 2.1).

| 4.7. | Sodium or potassium hydroxide solution, carbonate free: 0,2 mol/l |
| --- | --- |

4.7.

Sodium or potassium hydroxide solution, carbonate free: 0,2 mol/l

| 4.8. | Sulphuric acid: 0,25 mol/l |
| --- | --- |

4.8.

Sulphuric acid: 0,25 mol/l

for variant c (see note 2, Method 2.1).’

| 4.9. | Sodium or potassium hydroxide solution, carbonate free: 0,5 mol/l |
| --- | --- |

4.9.

Sodium or potassium hydroxide solution, carbonate free: 0,5 mol/l

| (g) | in Method 2.3.3, points 4.3 to 4.8 are replaced by the following:












  ‘4.3. | Sulphuric acid: 0,05 mol/l |
| --- | --- | --- |

(g)

in Method 2.3.3, points 4.3 to 4.8 are replaced by the following:

‘4.3.

Sulphuric acid: 0,05 mol/l

for variant a (see Method 2.1).

| 4.4. | Sodium or potassium hydroxide solution, carbonate free: 0,1 mol/l |
| --- | --- |

4.4.

Sodium or potassium hydroxide solution, carbonate free: 0,1 mol/l

| 4.5. | Sulphuric acid: 0,1 mol/l |
| --- | --- |

4.5.

Sulphuric acid: 0,1 mol/l

for variant b (see note 2, Method 2.1).

| 4.6. | Sodium or potassium hydroxide solution, carbonate free: 0,2 mol/l |
| --- | --- |

4.6.

Sodium or potassium hydroxide solution, carbonate free: 0,2 mol/l

| 4.7. | Sulphuric acid: 0,25 mol/l |
| --- | --- |

4.7.

Sulphuric acid: 0,25 mol/l

for variant c (see note 2, Method 2.1).’

| 4.8. | Sodium or potassium hydroxide solution, carbonate free: 0,5 mol/l |
| --- | --- |

4.8.

Sodium or potassium hydroxide solution, carbonate free: 0,5 mol/l

| (h) | in Method 2.4, point 4.8 is replaced by the following:






  ‘4.8. | Sulphuric acid: 0,05 mol/l’; |
| --- | --- | --- |

(h)

in Method 2.4, point 4.8 is replaced by the following:

‘4.8.

| (i) | Method 2.5 is amended as follows:






  (i) | point 4.2 is replaced by the following:






  ‘4.2. | Sulphuric acid solution, about 0,05 mol/l’; |
| --- | --- | --- | --- |

(i)

Method 2.5 is amended as follows:

(i)

point 4.2 is replaced by the following:

‘4.2.

| (ii) | in point 7.1, the second sentence is replaced by the following:

  ‘Make up the volumes to about 50 ml with water, add one drop of indicator (4.7) and neutralise, if necessary, with sulphuric acid 0,05 mol/l (4.2).’; |
| --- | --- |

(ii)

in point 7.1, the second sentence is replaced by the following:

‘Make up the volumes to about 50 ml with water, add one drop of indicator (4.7) and neutralise, if necessary, with sulphuric acid 0,05 mol/l (4.2).’;

| (iii) | point 7.3, the first paragraph is replaced by the following:

  ‘According to the presumed biuret content, transfer 25 or 50 ml from the solution mentioned in 7.2 with a pipette, place this quantity in a 100 ml graduated flask and neutralise if necessary with a 0,05 mol/l or a 0,1 mol/l reagent (4.2 or 4.3) as required, using methyl red as an indicator and add, with the same accuracy as that used when drawing up a calibration curve, 20 ml of the alkaline solution of potassium sodium tartrate (4.4) and 20 ml of the copper solution (4.5). Make up to volume, mix thoroughly and leave standing for 15 minutes at 30 (± 2) °C.’; |
| --- | --- |

(iii)

point 7.3, the first paragraph is replaced by the following:

‘According to the presumed biuret content, transfer 25 or 50 ml from the solution mentioned in 7.2 with a pipette, place this quantity in a 100 ml graduated flask and neutralise if necessary with a 0,05 mol/l or a 0,1 mol/l reagent (4.2 or 4.3) as required, using methyl red as an indicator and add, with the same accuracy as that used when drawing up a calibration curve, 20 ml of the alkaline solution of potassium sodium tartrate (4.4) and 20 ml of the copper solution (4.5). Make up to volume, mix thoroughly and leave standing for 15 minutes at 30 (± 2) °C.’;

| (j) | Method 2.6.1 is amended as follows:






  (i) | point 4.8 is replaced by the following:






  ‘4.8. | Standard solution of sulphuric acid: 0,1 mol/l’; |
| --- | --- | --- | --- |

(j)

Method 2.6.1 is amended as follows:

(i)

point 4.8 is replaced by the following:

‘4.8.

| (ii) | point 4.17 is replaced by the following:






  ‘4.17. | Standard sulphuric acid solution: 0,05 mol/l’; |
| --- | --- | --- |

(ii)

point 4.17 is replaced by the following:

‘4.17.

| (iii) | in point 7.1.1.2, the first sentence of the first paragraph is replaced by the following:

  ‘Transfer with a precision pipette, into the receiver of the apparatus, 50 ml of a standard solution of sulphuric acid 0,1 mol/l (4.8).’; |
| --- | --- |

(iii)

in point 7.1.1.2, the first sentence of the first paragraph is replaced by the following:

‘Transfer with a precision pipette, into the receiver of the apparatus, 50 ml of a standard solution of sulphuric acid 0,1 mol/l (4.8).’;

| (iv) | in point 7.1.1.4, the explanation of the element ‘a’ of the formula is replaced by the following:

  ‘a= ml of standard solution of sodium or potassium hydroxide 0,2 mol/l, used for the blank, carried out by pipetting into the receiver of the apparatus (5.1), 50 ml of standard solution of sulphuric acid 0,1 mol/l (4.8),’; |
| --- | --- |

(iv)

in point 7.1.1.4, the explanation of the element ‘a’ of the formula is replaced by the following:

‘a= ml of standard solution of sodium or potassium hydroxide 0,2 mol/l, used for the blank, carried out by pipetting into the receiver of the apparatus (5.1), 50 ml of standard solution of sulphuric acid 0,1 mol/l (4.8),’;

| (v) | in point 7.1.2.6, the explanation of the element ‘a’ of the formula is replaced by the following:

  ‘a= ml of standard solution of sodium or potassium hydroxide 0,2 mol/l, used for the blank, carried out by pipetting into the receiver of the apparatus (5.1), 50 ml of standard solution of sulphuric acid 0,1 mol/l (4.8),’; |
| --- | --- |

(v)

in point 7.1.2.6, the explanation of the element ‘a’ of the formula is replaced by the following:

‘a= ml of standard solution of sodium or potassium hydroxide 0,2 mol/l, used for the blank, carried out by pipetting into the receiver of the apparatus (5.1), 50 ml of standard solution of sulphuric acid 0,1 mol/l (4.8),’;

| (vi) | in point 7.2.2.4, the explanation of the element ‘a’ of the formula is replaced by the following:

  ‘a= ml of standard solution of sodium or potassium hydroxide 0,2 mol/l, used for the blank, carried out by pipetting into the receiver of the apparatus (5.1), 50 ml of standard solution of sulphuric acid 0,1 mol/l (4.8),’; |
| --- | --- |

(vi)

in point 7.2.2.4, the explanation of the element ‘a’ of the formula is replaced by the following:

‘a= ml of standard solution of sodium or potassium hydroxide 0,2 mol/l, used for the blank, carried out by pipetting into the receiver of the apparatus (5.1), 50 ml of standard solution of sulphuric acid 0,1 mol/l (4.8),’;

| (vii) | in point 7.2.3.2, the explanation of the element ‘a’ of the formula is replaced by the following:

  ‘a= ml of standard solution of sodium or potassium hydroxide 0,2 mol/l, used for the blank, carried out by pipetting into the receiver of the apparatus (5.1), 50 ml of standard solution of sulphuric acid 0,1 mol/l (4.8),’; |
| --- | --- |

(vii)

in point 7.2.3.2, the explanation of the element ‘a’ of the formula is replaced by the following:

‘a= ml of standard solution of sodium or potassium hydroxide 0,2 mol/l, used for the blank, carried out by pipetting into the receiver of the apparatus (5.1), 50 ml of standard solution of sulphuric acid 0,1 mol/l (4.8),’;

| (viii) | in point 7.2.5.2, the explanation of the element ‘a’ of the formula is replaced by the following:

  ‘a= ml of standard solution of sodium or potassium hydroxide 0,2 mol/l, used for the blank, carried out by pipetting into the receiver of the apparatus (5.1), 50 ml of standard solution of sulphuric acid 0,1 mol/l (4.8),’; |
| --- | --- |

(viii)

in point 7.2.5.2, the explanation of the element ‘a’ of the formula is replaced by the following:

‘a= ml of standard solution of sodium or potassium hydroxide 0,2 mol/l, used for the blank, carried out by pipetting into the receiver of the apparatus (5.1), 50 ml of standard solution of sulphuric acid 0,1 mol/l (4.8),’;

| (ix) | in point 7.2.5.3, the first three sentences are replaced by the following:

  ‘Transfer with a precision pipette, into the dry flask of the apparatus (5.2), an aliquot sample of the filtrate (7.2.1.1 or 7.2.1.2) containing 20 mg of ammoniacal nitrogen at the most. Then assemble the apparatus. Pipette, into the 300 ml Erlenmeyer, 50 ml of the standard sulphuric acid solution 0,05 mol/l (4.17) and enough distilled water for the level of the liquid to be approximately 5 cm above the opening of the delivery tube.’; |
| --- | --- |

(ix)

in point 7.2.5.3, the first three sentences are replaced by the following:

‘Transfer with a precision pipette, into the dry flask of the apparatus (5.2), an aliquot sample of the filtrate (7.2.1.1 or 7.2.1.2) containing 20 mg of ammoniacal nitrogen at the most. Then assemble the apparatus. Pipette, into the 300 ml Erlenmeyer, 50 ml of the standard sulphuric acid solution 0,05 mol/l (4.17) and enough distilled water for the level of the liquid to be approximately 5 cm above the opening of the delivery tube.’;

| (x) | in point 7.2.5.5, the explanation of the element ‘a’ of the formula is replaced by the following:

  ‘a= ml of standard solution of sodium or potassium hydroxide 0,1 mol/l, used for the blank, carried out by pipetting into the 300 ml Erlenmeyer of the apparatus (5.2), 50 ml of standard solution of sulphuric acid 0,05 mol/l (4.17),’; |
| --- | --- |

(x)

in point 7.2.5.5, the explanation of the element ‘a’ of the formula is replaced by the following:

‘a= ml of standard solution of sodium or potassium hydroxide 0,1 mol/l, used for the blank, carried out by pipetting into the 300 ml Erlenmeyer of the apparatus (5.2), 50 ml of standard solution of sulphuric acid 0,05 mol/l (4.17),’;

| (k) | Method 2.6.2 is amended as follows:






  (i) | point 4.6 is replaced by the following:






  ‘4.6. | Sulphuric acid solution 0,1 mol/l’; |
| --- | --- | --- | --- |

(k)

Method 2.6.2 is amended as follows:

(i)

point 4.6 is replaced by the following:

‘4.6.

| (ii) | point 4.14 is replaced by the following:






  ‘4.14. | Titrated sulphuric acid solution: 0,05 mol/l’; |
| --- | --- | --- |

(ii)

point 4.14 is replaced by the following:

‘4.14.

| (iii) | in point 7.2.4, the explanation of the element ‘a’ of the formula is replaced by the following:

  ‘a= ml of titrated 0,2 mol/l sodium or potassium hydroxide solution (4.8), used in the blank test, carried out by placing 50 ml of titrated 0,1 mol/l sulphuric acid solution into the receiver of the apparatus (4.6),’; |
| --- | --- |

(iii)

in point 7.2.4, the explanation of the element ‘a’ of the formula is replaced by the following:

‘a= ml of titrated 0,2 mol/l sodium or potassium hydroxide solution (4.8), used in the blank test, carried out by placing 50 ml of titrated 0,1 mol/l sulphuric acid solution into the receiver of the apparatus (4.6),’;

| (iv) | in point 7.3.3, the explanation of the element ‘a’ of the formula is replaced by the following:

  ‘a= ml of titrated 0,2 mol/l sodium or potassium hydroxide solution (4.8), used in the blank test, carried out by pipetting 50 ml of titrated 0,1 mol/l sulphuric acid solution (4.6) into the receiver of the apparatus,’; |
| --- | --- |

(iv)

in point 7.3.3, the explanation of the element ‘a’ of the formula is replaced by the following:

‘a= ml of titrated 0,2 mol/l sodium or potassium hydroxide solution (4.8), used in the blank test, carried out by pipetting 50 ml of titrated 0,1 mol/l sulphuric acid solution (4.6) into the receiver of the apparatus,’;

| (v) | in point 7.5.1, the third sentence of the first paragraph is replaced by the following:

  ‘Pipette into the 300 ml Erlenmeyer exactly 50 ml of titrated 0,05 mol/l sulphuric acid solution (4.14) and an amount of distilled water so that the level of the liquid is approximately 5 cm above the opening of the intake tube.’; |
| --- | --- |

(v)

in point 7.5.1, the third sentence of the first paragraph is replaced by the following:

‘Pipette into the 300 ml Erlenmeyer exactly 50 ml of titrated 0,05 mol/l sulphuric acid solution (4.14) and an amount of distilled water so that the level of the liquid is approximately 5 cm above the opening of the intake tube.’;

| (vi) | in point 7.5.3, the explanation of the element ‘a’ of the formula is replaced by the following:

  ‘a= ml of titrated 0,1 mol/l sodium or potassium hydroxide solution (4.17), used in the blank test, carried out by pipetting into the 300 ml Erlenmeyer of the apparatus (5.2) 50 ml of titrated 0,05 mol/l sulphuric acid solution (4.14).’. |
| --- | --- |

(vi)

in point 7.5.3, the explanation of the element ‘a’ of the formula is replaced by the following:

‘a= ml of titrated 0,1 mol/l sodium or potassium hydroxide solution (4.17), used in the blank test, carried out by pipetting into the 300 ml Erlenmeyer of the apparatus (5.2) 50 ml of titrated 0,05 mol/l sulphuric acid solution (4.14).’.

| 2. | Method 3 is amended as follows:






  (a) | in Method 3.1.5.1, point 4.2, the first three sentences are replaced by the following:

  ‘Citric acid (C6H8O7.H2O): 173 g per litre.
  Ammonia: 42 g per litre of ammoniacal nitrogen.
  Sulphuric acid 0,25 mol/l
  pH between 9,4 and 9,7.’; |
| --- | --- | --- |

2.

Method 3 is amended as follows:

(a)

in Method 3.1.5.1, point 4.2, the first three sentences are replaced by the following:

‘Citric acid (C6H8O7.H2O): 173 g per litre.

Ammonia: 42 g per litre of ammoniacal nitrogen.

Sulphuric acid 0,25 mol/l

pH between 9,4 and 9,7.’;

| (b) | in Method 3.1.5.3, point 4.1.2, the formula after the second paragraph of the Note is replaced by the following:

  ‘1 ml of H2SO4 0,25 mol/l = 0,008516 g of NH3
  ’. |
| --- | --- |

(b)

in Method 3.1.5.3, point 4.1.2, the formula after the second paragraph of the Note is replaced by the following:

‘1 ml of H2SO4 0,25 mol/l = 0,008516 g of NH3
  ’.

| 3. | Method 8 is amended as follows:






  (a) | in Method 8.5, point 8, the second formula is replaced by the following:



  ; |
| --- | --- | --- |

3.

Method 8 is amended as follows:

(a)

in Method 8.5, point 8, the second formula is replaced by the following:

Formula
  ;

| (b) | in Method 8.6, point 3 is replaced by the following:
  ‘3.   Principle
  Precipitation of the calcium contained in an aliquot of the extraction solution in the form of an oxalate, after separation and dissolution of the latter, by titration of oxalic acid using potassium permanganate’. |
| --- | --- |

(b)

in Method 8.6, point 3 is replaced by the following:

‘3.   Principle

Precipitation of the calcium contained in an aliquot of the extraction solution in the form of an oxalate, after separation and dissolution of the latter, by titration of oxalic acid using potassium permanganate’.

---