# Patent

## Title
Thermodynamically stable form of a tosylate salt

## Abstract

The present invention relates to a novel form, thermodynamically stable at room temperature, of the tosylate salt of 4-{4-[({[4-chloro-3-(trifluoromethyl)phenyl]amino}carbonyl)amino]phenoxy}-N-methylpyridine-2-carboxamide, to processes for its preparation, to medicaments comprising it and to its use in the control of disorders.

## Background
RELATED APPLICATIONS/PATENTS AND INCORPORATION BY REFERENCE
This application is a National Stage Application filed under 35 U.S.C. §371 based on International Application No. PCT/EP2005/010119, filed Sep. 20, 2005, which claims priority to European Patent Application Number 04023130.0, filed Sep. 29, 2004, the entire contents each of which are incorporated herein by reference.
The foregoing applications, and all documents cited therein and all documents cited or referenced therein, and all documents cited or referenced herein, including any U.S. or foreign patents or published patent applications, International patent applications, as well as, any non-patent literature references and any manufacturer's instructions, are hereby expressly incorporated herein by reference.
The present invention relates to a novel form, thermodynamically stable at room temperature, of the tosylate salt of 4-{4-[({[4-chloro-3-(trifluoromethyl)phenyl]amino}carbonyl)amino]phenoxy}-N-methylpyridine-2-carboxamide, to processes for its preparation, to pharmaceutical compositions comprising it and to its use in the control of disorders.
The tosylate salt of 4-{4-[({[4-chloro-3-(trifluoromethyl)phenyl]amino}carbonyl)amino]phenoxy}-N-methylpyridine-2-carboxamide is mentioned in WO 03/068228 and WO 03/047579 and corresponds to the compound of the formula (I):




WO 03/068228 relates, inter alia, to the use of the compound of the formula (I) for the treatment of disorders in which angiogenesis plays an important role, for example in tumor growth. WO 03/047579 relates to arylureas in combination with cytotoxic or cytostatic compounds for the treatment of cancer.
The compound 4-{4-[({[4-chloro-3-(trifluoromethyl)phenyl]amino}carbonyl)amino]phenoxy}-N-methylpyridine-2-carboxamide is described in WO 00/42012 and corresponds to the compound of the formula (II):




The compounds and their salts, disclosed in WO 00/42012, for example tosylates, are described there as inhibitors of the enzyme Raf kinase and may be used for the treatment of disorders, for example cancer.



BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 is DSC—and TGA-thermograms of compound (I).
FIG. 2 is X-Ray diffraction patterns of compound (I).
FIG. 3 is IR spectra of compound (I).
FIG. 4 is Raman spectra of compound (I).
FIG. 5 is FIR spectra of compound (I).
FIG. 6 is NIR spectra of compound (I).
The compound of the formula (II) is prepared in the manner described in WO 00/42012. The compound of the formula (I) is prepared according to a general standard method for the preparation of tosylate salts, as described in example 1 of the working examples. In this method, the compound of the formula (I) is obtained in one crystal polymorph which is referred to hereinbelow as polymorph II. Polymorph II has a transition point of 194° C. and a characteristic X-ray diffractogram, IR spectrum, Raman spectrum, FIR spectrum and NIR spectrum (Tab. 1-6, FIG. 1-6). It has been found that polymorph II is metastable.
Surprisingly, two further polymorphs and two solvates of the compound of the formula (I) have been found. The compound of the formula (I) in the polymorph I melts under decomposition at 223-231° C., the compound of the formula (I) in the polymorph III melts at 187-190° C. The monomethanol solvate of the compound of the formula (I) contains 4.8% methanol and the monoethanol solvate of the compound of the formula (I) 6.7% ethanol. The inventive polymorph I of the compound of the formula (I) is thermodynamically stable at room temperature and is storage-stable even after processing via suspensions and is therefore particularly suitable for use in pharmaceutical formulations, for example suspensions or creams, but also in other preparations which are prepared via suspended active ingredient, for example in aqueous granulation or wet grinding.
The present invention provides the compound of the formula (I) in the polymorph I. The inventive use of the compound of the formula (I) in the stable polymorph I ensures that an undesired conversion to another polymorph and an associated change in the properties of the compound of the formula (I), for example solubility or bioavailability, are prevented. This increases the safety and quality of preparations comprising the compound of the formula (I) and the risk to the patient is reduced.
Polymorph I of the compound of the formula (I), in comparison to polymorph II, polymorph III, the ethanol and methanol solvate, has a clearly differentiable X-ray diffractogram, NIR spectrum, FIR spectrum and Raman spectrum (FIG. 2-6). The compound of the formula (I) in the polymorph I melts under decomposition at 223-231° C. and is thus clearly differentiable from polymorph II (conversion point 194° C.) and polymorph III (melting point 187-190° C.). Unlike those solvent-free forms, the ethanol solvate of the compound of the formula (I) and the methanol solvate of the compound of the formula (I) have losses of mass in thermogravimetric analysis (TGA) of 6.7% and 4.8% respectively (FIG. 1).
The inventive compound of the formula (I) in the polymorph I is used in high purity in pharmaceutical formulations. For reasons of stability, a pharmaceutical formulation comprises the compound of the formula (I) mainly in the polymorph I and no significant fractions of another form, for example of another polymorph or of a solvate of the compound of the formula (I). The pharmaceutical composition preferably contains more than 90 percent by weight, more preferably more than 95 percent by weight, of the compound of the formula (I) in the polymorph I related to the total amount of the compound of the formula (I) present in the composition.
Method for Treatment:
The present invention further provides the use of the compound of the formula (I) in the polymorph I for the treatment of disorders. Preference is given to using it for the treatment of disorders which feature abnormal angiogenesis or hyperpermeability processes, bone marrow diseases, for example leukemia, or treatment of carcinoma, for example carcinoma of the lung, of the pancreas, of the thyroid gland, of the kidney or of the intestine, or for the treatment of carcinogenic cell growth.
The present invention further provides the use of the compound of the formula (I) in the polymorph I for the preparation of a pharmaceutical composition for the treatment of disorders. Preference is given to using it for the treatment of disorders which feature abnormal angiogenesis or hyperpermeability processes, bone marrow diseases, for example leukemia, or treatment of carcinoma, for example carcinoma of the lung, of the pancreas, of the thyroid gland, of the kidney or of the intestine, or for the treatment of carcinogenic cell growth.
The compound of the formula (I) in the polymorph I of the present invention can be used to treat, modulate and/or prevent any disease or condition mediated by one or more cellular signal transduction pathways involving raf, VEGFR, PDGFR, p38, and/or flt-3 kinases.
The term “mediated” indicates, e.g., that the signaling molecule is part of the pathway which is aberrant or disturbed in the disease and/or condition.
While not wishing to be bound by any theory or mechanism of action, it has been found that compounds of the present invention possess the ability to modulate raf, VEGFR, PDGFR, p38, and/or flt-3 kinase activity. The methods of the present invention, however, are not limited to any particular mechanism or how the compounds achieve their therapeutic effect.
By the term “modulate,” it is meant that the functional activity of the pathway (or a component of it) is changed in comparison to its normal activity in the absence of the compound. This effect includes any quality or degree of modulation, including, increasing, agonizing, augmenting, enhancing, facilitating, stimulating, decreasing, blocking, inhibiting, reducing, diminishing, antagonizing, etc.
By the phrase “kinase activity,” it is meant a catalytic activity in which a phosphate from adenosine triphosphate (ATP) is transferred to an amino acid residue (e.g., serine, threonine, or tyrosine) in a protein substrate. A compound can modulate kinase activity, e.g., inhibiting it by directly competing with ATP for the ATP-binding pocket of the kinase, by producing a conformational change in the enzyme's structure that affects its activity (e.g., by disrupting the biologically-active three-dimensional structure), etc. Kinase activity can be determined routinely using conventional assay methods. Kinase assays typically comprise the kinase enzyme, substrates, buffers, and components of a detection system.
A disease or condition “mediated” by raf, VEGFR, PDGFR, p38, and/or flt-3 indicates that one of these receptors is a part of a signal transduction pathway that is involved in any aspect of the disease phenotype (e.g., where a defect in the receptor itself is involved in “causing” the disease; where stimulation of the receptor by its ligand induces cell motility, migration, and/or proliferation that produces a disease phenotype; where receptor stimulation or phosphorylation results in restonosis; any functional activity of raf, VEGFR, PDGFR, p38, and/or flt-3 that, when inappropriately expressed, results in a disease symptom and/or phenotype).
The term “treating” is used conventionally, e.g., the management or care of a subject for the purpose of combating, alleviating, reducing, relieving, improving the condition of, etc., of a disease or disorder.
Diseases and conditions that can be treated include any of those mentioned above and below, as well as:
Raf associated diseases include, e.g., cell-proliferation disorders, cancer, tumors, etc.;
VEGFR-2 associated diseases include, e.g., cancer, tumor growth, inflammatory disease, rheumatoid arthritis, retinopathy, psoriasis, glomerulonephritis, asthma, chronic bronchitis, atherosclerosis, transplant rejection, conditions involving angiogenesis, etc.;

VEGFR-3 associated diseases include, e.g., cancer, corneal disease, inflamed cornea, corneal transplantation, lymphatic hyperplasia, conditions involving lymphangiogenesis, etc.;

PDGFR-beta associated diseases include, e.g., diseases or conditions characterized by cell proliferation, cell matrix production, cell movement, and/or extracellular matrix production. Specific examples, include, e.g., tumors, malignancies, cancer, metastasis, chronic myeloid leukemia, inflammation, renal disease, diabetic nephropathy, mesangial proliferative glomerulonephritis, fibrotic conditions, atherosclerosis, restenosis, hypertension-related arteriosclerosis, venous bypass graft arteriosclerosis, scleroderma, interstitial pulmonary diseases, synovial disorders, arthritis, leukemias, lymphomas, etc;

Flt-3 associated diseases include, e.g., immune-related disorders, blood cell disorders, conditions involving hematopoietic cell development (e.g., T-cells, B-cells, dendritic cells, cancer, anemia, HIV, acquired immune deficiency syndrome, etc.

p38 associated diseases include inflammatory disorders, immunomodulatory disorders, and other disorders that have been linked to abnormal cytokine production, especially TNF-alpha, or abnormal MMP activity. These disorders include, but are not limited to, rheumatoid arthritis, COPD, osteoporosis, Crohn's disease and psoriasis.

Methods of the present invention include modulating tumor cell proliferation, including inhibiting cell proliferation. The latter indicates that the growth and/or differentiation of tumor cells is reduced, decreased, diminished, slowed, etc. The term “proliferation” includes any process which relates to cell growth and division, and includes differentiation and apoptosis. As discussed above, raf kinases play a key role in the activation of the cytoplasmic signaling cascade involved in cell proliferation, differentiation, and apoptosis. For example, studies have found that inhibiting c-raf by anti-sense oligonucleotides can block cell proliferation. Any amount of inhibition is considered therapeutic.
Methods of the present invention also include treating mammalian hyper-proliferative disorders. Hyper-proliferative disorders include but are not limited to solid tumors, such as cancers of the breast, respiratory tract, brain, reproductive organs, digestive tract, urinary tract, eye, liver, skin, head and neck, thyroid, parathyroid and their distant metastases. Those disorders also include lymphomas, sarcomas, and leukemias.
Any tumor or cancer can be treated, including, but not limited to, cancers having one or more mutations in raf, ras, VEFGR, PDGFR, p38, and/or flt-3, as well as any upstream or downstream member of the signaling pathways of which they are a part. As discussed earlier, a cancer can be treated with a compound of the present invention irrespective of the mechanism which is responsible for it.
Cancers of any organ can be treated, including cancers of, but are not limited to, e.g., colon, pancreas, breast, prostate, bone, liver, kidney, lung, testes, skin, pancreas, stomach, colorectal cancer, renal cell carcinoma, hepatocellular carcinoma, melanoma, etc.
Examples of breast cancer include, but are not limited to, invasive ductal carcinoma, invasive lobular carcinoma, ductal carcinoma in situ, and lobular carcinoma in situ.
Examples of cancers of the respiratory tract include, but are not limited to, small-cell and non-small-cell lung carcinoma, as well as bronchial adenoma and pleuropulmonary blastoma.
Examples of brain cancers include, but are not limited to, brain stem and hypophtalmic glioma, cerebellar and cerebral astrocytoma, medulloblastoma, ependymoma, as well as neuroectodermal and pineal tumor.
Tumors of the male reproductive organs include, but are not limited to, prostate and testicular cancer. Tumors of the female reproductive organs include, but are not limited to, endometrial, cervical, ovarian, vaginal, and vulvar cancer, as well as sarcoma of the uterus.
Tumors of the digestive tract include, but are not limited to, anal, colon, colorectal, esophageal, gallbladder, gastric, pancreatic, rectal, small-intestine, and salivary gland cancers.
Tumors of the urinary tract include, but are not limited to, bladder, penile, kidney, renal pelvis, ureter, and urethral cancers.
Eye cancers include, but are not limited to, intraocular melanoma and retinoblastoma.
Examples of liver cancers include, but are not limited to, hepatocellular carcinoma (liver cell carcinomas with or without fibrolamellar variant), cholangiocarcinoma (intrahepatic bile duct carcinoma), and mixed hepatocellular cholangiocarcinoma.
Skin cancers include, but are not limited to, squamous cell carcinoma, Kaposi's sarcoma, malignant melanoma, Merkel cell skin cancer, and non-melanoma skin cancer.
Head-and-neck cancers include, but are not limited to, laryngeal, hypopharyngeal, nasopharyngeal, and/or oropharyngeal cancers, and lip and oral cavity cancer.
Lymphomas include, but are not limited to, AIDS-related lymphoma, non-Hodgkin's lymphoma, cutaneous T-cell lymphoma, Hodgkin's disease, and lymphoma of the central nervous system.
Sarcomas include, but are not limited to, sarcoma of the soft tissue, osteosarcoma, malignant fibrous histiocytoma, lymphosarcoma, and rhabdomyosarcoma.
Leukemias include, but are not limited to, acute or chronic, acute myeloid leukemia, acute lymphoblastic leukemia, acute Lymphocytic Leukemia, chronic lymphocytic leukemia, chronic myelogenous leukemia, hairy cell leukemia, undifferentiated AML, promyelocytic leukemia, myelomonocytic leukemia, monocytic leukemia, or erythroleukemia, megakaryoblastic leukemia, etc.
In addition to inhibiting the proliferation of tumor cells, compounds of the present invention can also cause tumor regression, e.g., a decrease in the size of a tumor, or in the extent of cancer in the body.
The present invention also relates to methods of modulating angiogenesis and/or lymphangiogenesis in a system comprising cells, comprising administering to the system an effective amount of a compound described herein. A system comprising cells can be an in vivo system, such as a tumor in a patient, isolated organs, tissues, or cells, in vitro assays systems (CAM, BCE, etc), animal models (e.g., in vivo, subcutaneous, cancer models), hosts in need of treatment (e.g., hosts suffering from diseases having angiogenic and/or lymphangiogenic component, such as cancer), etc.
Inappropriate and ectopic expression of angiogenesis (e.g., abnormal angiogenesis) can be deleterious to an organism. A number of pathological conditions are associated with the growth of extraneous blood vessels. These include, e.g., diabetic retinopathy, neovascular glaucoma, psoriasis, retrolental fibroplasias, angiofibroma, inflammation, etc. In addition, the increased blood supply associated with cancerous and neoplastic tissue, encourages growth, leading to rapid tumor enlargement and metastasis. Moreover, the growth of new blood and lymph vessels in a tumor provides an escape route for renegade cells, encouraging metastasis and the consequence spread of the cancer.
Methods of the present invention also relate to treating and/or preventing disorders or conditions associated with, or resulting from, vascular hyperpermeability.
For example, VEGF increases endothelial cell permeability. As a consequence, any condition which results in the release of VEGF, especially in higher than normal amounts, can be associated with vascular hyperpermeability and its accompanying deleterious effects. The present invention, however, provides for the treatment or prevention of any condition or disorder associated with, or resulting from, vascular hyperpermeability, regardless of the mechanism of action.
Edema formation is a life-threatening complication of various diseases of the central nervous system, including head injury, tumors, stroke, hypoxia, and high altitude sickness. The underlying cause of edema is vascular hyperpermeability. Compounds of the present invention can be utilized to treat and/or prevent vascular hyperpermeability, thereby treating and/or preventing edema, and the deleterious effects associated with it.
Other hyperpermeability conditions (or conditions that produce vascular hypermeability), include, but are not limited to, tissue edema (e.g., lung, kidney, brain, etc.), vasogenic brain edema, chronic inflammation, wound healing, ischemia, tumors, atherosclerosis, peripheral vascular disease, ascites, effusions, exudates, nephrotic edema, primary glomerular disease, peripheral artery disease, diabetic retinopathy, diabetic retinal disease, obstruction of respiratory airways during asthma and other pulmonary disorders, circulatory collapse in sepsis, acute lung injury, acute respiratory distress syndrome, etc.
Assays for vascular permeability can be done routinely, e.g., Heiss et al., J. Clin. Invest., 98:1400-1408, 1996; Fischer et al., Am. J. Physiol., 276(4 Pt 1):C812-20, 1999; Fischer et al., Am. J. Physiol. Cell. Physiol., 279:C935-C944, 2000.
The compound of the formula (I) in the polymorph I of this invention also has a broad therapeutic activity to treat or prevent the progression of a broad array of diseases, such as inflammatory conditions, coronary restenosis, tumor-associated angiogenesis, atherosclerosis, autoimmune diseases, inflammation, certain kidney diseases associated with proliferation of glomerular or mesangial cells, and ocular diseases associated with retinal vessel proliferation. psoriasis, hepatic cirrhosis, diabetes, atherosclerosis, restenosis, vascular graft restenosis, in-stent stenosis, angiogenesis, ocular diseases, pulmonary fibrosis, obliterative bronchiolitis, glomerular nephritis, rheumatoid arthritis.
The present invention also provides for treating, preventing, modulating, etc., one or more of the following conditions in humans and/or other mammals: retinopathy, including diabetic retinopathy, ischemic retinal-vein occlusion, retinopathy of prematurity and age related macular degeneration; rheumatoid arthritis, psoriasis, or bullous disorder associated with subepidermal blister formation, including bullous pemphigoid, erythema multiforme, or dermatitis herpetiformis, rheumatic fever, bone resorption, postmenopausal osteoporosis, sepsis, gram negative sepsis, septic shock, endotoxic shock, toxic shock syndrome, systemic inflammatory response syndrome, inflammatory bowel disease (Crohn's disease and ulcerative colitis), Jarisch-Herxheimer reaction, asthma, adult respiratory distress syndrome, acute pulmonary fibrotic disease, pulmonary sarcoidosis, allergic respiratory disease, silicosis, coal worker's pneumoconiosis, alveolar injury, hepatic failure, liver disease during acute inflammation, severe alcoholic hepatitis, malaria (Plasmodium falciparum malaria and cerebral malaria), non-insulin-dependent diabetes mellitus (NIDDM), congestive heart failure, damage following heart disease, atherosclerosis, Alzheimer's disease, acute encephalitis, brain injury, multiple sclerosis (demyelation and oligiodendrocyte loss in multiple sclerosis), advanced cancer, lymphoid malignancy, pancreatitis, impaired wound healing in infection, inflammation and cancer, myelodysplastic syndromes, systemic lupus erythematosus, biliary cirrhosis, bowel necrosis, radiation injury/toxicity following administration of monoclonal antibodies, host-versus-graft reaction (ischemia reperfusion injury and allograft rejections of kidney, liver, heart, and skin), lung allograft rejection (obliterative bronchitis), or complications due to total hip replacement, ad an infectious disease selected from tuberculosis, Helicobacter pylori infection during peptic ulcer disease, Chaga's disease resulting from Trypanosoma cruzi infection, effects of Shiga-like toxin resulting from E. coli infection, effects of enterotoxin A resulting from Staphylococcus infection, meningococcal infection, and infections from Borrelia burgdorferi, Treponema pallidum, cytomegalovirus, influenza virus, Theiler's encephalomyelitis virus, and the human immunodeficiency virus (HIV), papilloma, blastoglioma, Kaposi's sarcoma, melanoma, lung cancer, ovarian cancer, prostate cancer, squamous cell carcinoma, astrocytoma, head cancer, neck cancer, bladder cancer, breast cancer, colorectal cancer, thyroid cancer, pancreatic cancer, gastric cancer, hepatocellular carcinoma, leukemia, lymphoma, Hodgkin's disease, Burkitt's disease, arthritis, rheumatoid arthritis, diabetic retinopathy, angiogenesis, restenosis, in-stent restenosis, vascular graft restenosis, pulmonary fibrosis, hepatic cirrhosis, atherosclerosis, glomerulonophritis, diabetic nephropathy, thrombic micoangiopathy syndromes, transplant rejection, psoriasis, diabetes, wound healing, inflammation, and neurodegenerative diseases. hyperimmune disorders, hemangioma, myocardial angiogenesis, coronary and cerebral collateral vascularization, ischemia, corneal disease, rubeosis, neovascular glaucoma, macular degeneration retinopathy of prematurity, wound healing, ulcer Helicobacter related diseases, fractures, endometriosis, a diabetic condition, cat scratch fever, thyroid hyperplasia, asthma or edema following burns, trauma, chronic lung disease, stroke, polyps, cysts, synovitis, chronic and allergic inflammation, ovarian hyperstimulation syndrome, pulmonary and cerebral edema, keloid, fibrosis, cirrhosis, carpal tunnel syndrome, adult respiratory distress syndrome, ascites, an ocular condition, a cardiovascular condition, Crow-Fukase (POEMS) disease, Crohn's disease, glomerulonophritis, osteoarthritis, multiple sclerosis, graft rejection, Lyme disease, sepsis, von Hippel Lindau disease, pemphigoid, Paget's disease, polycystic kidney disease, sarcoidosis, throiditis, hyperviscosity syndrome, Osler-Weber-Rendu disease, chronic occlusive pulmonary disease, radiation, hypoxia, preeclampsia, menometrorrhagia, endometriosis, infection by Herpes simplex, ischemic retinopathy, corneal angiogenesis, Herpes Zoster, human immunodeficiency virus, parapoxvirus, protozoa, toxoplasmosis, and tumor-associated effusions and edema.
The present invention further provides a method for the prevention or treatment of diseases, especially of the aforementioned diseases, using an effective amount of the compound of the formula (I) in the polymorph I.
Combination with Other Pharmaceutical Agents:
The compound of the formula (I) in the polymorph I of this invention can be administered as the sole pharmaceutical agent or in combination with one or more other pharmaceutical agents where the combination causes no unacceptable adverse effects. This may be of particular relevance for the treatment of hyper-proliferative diseases such as cancer. In this instance, the compound of this invention can be combined with known cytotoxic agents, signal transduction inhibitors, with other anti-cancer agents, or with antiemetics, as well as with admixtures and combinations thereof.
In one embodiment, the compound of the formula (I) in the polymorph I of the present invention can be combined with cytotoxic anti-cancer agents. Examples of such agents can be found in the 11th Edition of the Merck Index (1996). These agents include, by no way of limitation, asparaginase, bleomycin, carboplatin, carmustine, chlorambucil, cisplatin, colaspase, cyclophosphamide, cytarabine, dacarbazine, dactinomycin, daunorubicin, doxorubicin (adriamycine), epirubicin, etoposide, 5-fluorouracil, hexamethylmelamine, hydroxyurea, ifosfamide, irinotecan, leucovorin, lomustine, mechlorethamine, 6-mercaptopurine, mesna, methotrexate, mitomycin C, mitoxantrone, prednisolone, prednisone, procarbazine, raloxifen, streptozocin, tamoxifen, thioguanine, topotecan, vinblastine, vincristine, and vindesine.
Other cytotoxic drugs suitable for use with the compounds of the invention include, but are not limited to, those compounds acknowledged to be used in the treatment of neoplastic diseases in Goodman and Gilman's The Pharmacological Basis of Therapeutics (Ninth Edition, 1996, McGraw-Hill). These agents include, by no way of limitation, aminoglutethimide, L-asparaginase, azathioprine, 5-azacytidine cladribine, busulfan, diethylstilbestrol, 2′,2′-difluorodeoxycytidine, docetaxel, erythrohydroxynonyladenine, ethinyl estradiol, 5-fluorodeoxyuridine, 5-fluorodeoxyuridine monophosphate, fludarabine phosphate, fluoxymesterone, flutamide, hydroxyprogesterone caproate, idarubicin, interferon, medroxyprogesterone acetate, megestrol acetate, melphalan, mitotane, paclitaxel, pentostatin, N-phosphonoacetyl-L-aspartate (PALA), plicamycin, semustine, teniposide, testosterone propionate, thiotepa, trimethylmelamine, uridine, and vinorelbine.
In another embodiment, the compound of the formula (I) in the polymorph I of the present invention can be combined with antiemetics. Antiemetics suitable for use with the compounds of the invention include, but are not limited to, antihistamines, H1 receptor blockers, 5-HT3 antagonists, neuroleptics, anticholinergics, dopamine antagonists, serotonin antagonists, glucocorticoids or cannabinoids. These agents include, by no way of limitation, meclozin, dimenhydrinate, phenothiazin derivatives (e.g. thiethylperazin, triflupromazin), benzamide or benzimidazolon derivatives (e.g. metoclopramid, bromoprid, domperidon), butyrophenones, scopalamin, pyridoxine, chlorphenoxamin, granisetron, ondansetron, tropisetron, and dexamethason. Preference is given to the antiemetics: granisetron, ondansetron, tropisetron, or dexamethason.
“Combination” mean for the purposes of the invention not only a dosage form which contains all the components (so-called fixed combinations), and combination packs containing the components separate from one another, but also components which are administered simultaneously or sequentially, as long as they are employed for the prophylaxis or treatment of the same disease.
The active ingredients of the combination according to the invention can be converted in a known manner into the usual formulations, which may be liquid or solid formulations. Examples are tablets, coated tablets, pills, capsules, granules, aerosols, syrups, emulsions, suspensions, solutions.
Since the combination according to the invention is well tolerated and in some cases is effective even in low dosages, a wide range of formulation variants is possible. Thus, one possibility is to formulate the individual active ingredients of the combination according to the invention separately. In this case, it is not absolutely necessary for the individual active ingredients to be taken at the same time; on the contrary, sequential intake may be advantageous to achieve optimal effects. It is appropriate with such separate administration to combine the formulations of the individual active ingredients, for example tablets or capsules, simultaneously together in a suitable primary packaging. The active ingredients are present in the primary packaging in each case in separate containers which may be, for example, tubes, bottles or blister packs. Such separate packaging of the components in the joint primary packaging is also referred to as a kit.
Further formulation variants which are suitable and preferred for the combination according to the invention are also fixed combinations. “Fixed combination” is intended here to mean pharmaceutical forms in which the components are present together in a fixed ratio of amounts. Such fixed combinations may be, for example, in the form of oral solutions, but they are preferably solid oral pharmaceutical preparations, e.g. capsules or tablets.
Pharmaceutical Compositions:
This invention also relates to pharmaceutical compositions containing the compound of the formula (I) in the polymorph I of the present invention and methods of administering to a patient in need thereof a pharmaceutical composition of this invention.
A patient, for the purpose of this invention, is a mammal, including a human, in need of treatment for the particular condition or disease.
The pharmaceutical compositions of the present invention are comprised of a pharmaceutically acceptable carrier and a pharmaceutically effective amount of the compound of the formula (I) in the polymorph I of the present invention.
A pharmaceutically acceptable carrier is any carrier which is relatively non-toxic and innocuous to a patient at concentrations consistent with effective activity of the active ingredient so that any side effects ascribable to the carrier do not vitiate the beneficial effects of the active ingredient.
A pharmaceutically effective amount of compound is that amount which produces a result or exerts an influence on the particular condition being treated.
The compound of the formula (I) in the polymorph I may be administered in a suitable manner, for example by the oral, parenteral, pulmonary, nasal, sublingual, lingual, buccal, rectal, dermal, transdermal, conjunctival or optic route, or as an implant or stent.
For these application routes, the inventive compounds may be administered in suitable administration forms.
For oral administration, suitable administration forms are those which function according to the prior art and deliver the compound of the formula (I) in the polymorph I in a rapid and/or modified manner, for example tablets (noncoated or coated tablets, for example with coatings which are resistant to gastric juice or have retarded dissolution or are insoluble and which control the release of the inventive compound), tablets which disintegrate rapidly in the oral cavity or films/wafers, films/lyophilizates, capsules (for example hard or soft gelatin capsules), sugar-coated tablets, granules, pellets, powders, suspensions or aerosols.
Parenteral administration can take place with avoidance of an absorption step (for example in an intravenous, intraarterial, intracardiac, intraspinal or intralumbar manner) or with inclusion of an absorption (for example in an intramuscular, subcutaneous, intracutaneous, percutaneous or intraperitoneal manner). Administration forms suitable for parenteral application include injection and infusion preparations in the form of suspensions, lyophilizates or sterile powders.
Suitable for the other administration routes are, for example, medicinal forms for inhalation (including powder inhalers, nebulizers), tablets for lingual, sublingual or buccal administration, films/wafers or capsules, suppositories, preparations for the ear or eye, vaginal capsules, aqueous suspensions (lotions, shaking mixtures), lipophilic suspensions, ointments, creams, transdermal therapeutic systems (for example patches), pastes, dusting powders, implants or stents.
The inventive compound may be converted to the application forms listed. This may take place in a known manner by mixing with inert, nontoxic, pharmaceutically suitable excipients. These excipients include carriers (for example, microcrystalline cellulose, lactose, mannitol), solvents (for example, liquid polyethylene glycols), emulsifiers and dispersants or wetting agents (for example, sodium dodecylsulfate, polyoxysorbitan oleate), binders (for example, polyvinylpyrrolidone), synthetic and natural polymers (for example, albumin), stabilizers (for example, antioxidants such as ascorbic acid), dyes (for example, inorganic pigments such as iron oxides) and substances for masking flavors and/or odors.
The present invention further provides medicaments which comprise at least the compound of the formula (I) in the polymorph I, typically together with one or more inert, nontoxic, pharmaceutically suitable excipients, for example binders, fillers, etc, and to the use thereof for the aforementioned purposes.
Dosage of the Pharmaceutical Compositions of the Present Invention:
Based upon standard laboratory techniques known to evaluate compounds useful for the treatment of any of the aforementioned disorders, by standard toxicity tests and by standard pharmacological assays for the determination of treatment of the conditions identified above in mammals, and by comparison of these results with the results of known medicaments that are used to treat these conditions, the effective dosage of the compounds of this invention can readily be determined for treatment of each desired indication. The amount of the active ingredient to be administered in the treatment of one of these conditions can vary widely according to such considerations as the particular compound and dosage unit employed, the mode of administration, the period of treatment, the age and sex of the patient treated, and the nature and extent of the condition treated.
The total amount of the active ingredient to be administered can range from about 0.001 mg/kg to about 200 mg/kg, and preferably from about 0.1 mg/kg to about 50 mg/kg body weight per day. A unit dosage may preferably contain from about 5 mg to about 4000 mg of active ingredient, and can be administered one or more times per day. The daily dosage for oral administration will preferably be from 0.1 to 50 mg/kg of total body weight. The daily dosage for administration by injection, including intravenous, intramuscular, subcutaneous and parenteral injections, and use of infusion techniques will preferably be from 0.1 to 10 mg/kg of total body weight. The daily rectal dosage regimen will preferably be from 0.1 to 50 mg/kg of total body weight. The daily vaginal dosage regimen will preferably be from 0.1 to 50 mg/kg of total body weight. The daily topical dosage regimen will preferably be from 0.1 to 10 mg/kg administered between one to four times daily. The transdermal concentration will preferably be that required to maintain a daily dose of from 0.1 to 10 mg/kg. The daily inhalation dosage regimen will preferably be from 0.1 to 10 mg/kg of total body weight. Other dosages and amounts can be selected routinely.
Nevertheless, it may in some cases be advantageous to deviate from the amounts specified, depending on body weight, administration route, individual behavior toward the active ingredient, type of preparation and time or interval over which the administration is effected. For instance, less than the aforementioned minimum amounts may be sufficient in some cases, while the upper limit specified has to be exceeded in other cases. In the case of administration of relatively large amounts, it may be advisable to divide these into several individual doses over the day.
Process for Preparing:
The invention further provides a process for preparing the compound of the formula (I) in the polymorph I, by effecting the compound of the formula (I) in the polymorph II, obtained as described in example 1, in an inert solvent at a temperature of, for example, from 50° C. up to the reflux temperature of the solvent, preferably from 60 to 80° C., in the absence of crystals of a solvate of the compound of the formula (I), for example in the absence of crystals of the methanol solvate or the ethanol solvate of the compound of formula (I), for up to one day. The mixture is cooled to from −30° C. to room temperature, preferably from −25° C. to 10° C., and the crystals are isolated and dried. The compound of the formula (I) is thus obtained in the polymorph I.
The invention likewise provides a process for preparing the compound of the formula (I) in the polymorph I, by effecting the compound of the formula (I) in the polymorph II, obtained as described in example 1, in an inert solvent at a temperature of, for example, from 10° C. up to the reflux temperature of the solvent, preferably at room temperature, for up to one day. Subsequently, the mixture is seeded with crystals of the compound of the formula (I) in the polymorph I and stirred or shaken, for example at room temperature, for from 1 hour to 14 days, preferably from 2 hours to 7 days. The crystals are isolated and dried. The compound of the formula (I) is thus obtained in the polymorph I.
The invention likewise provides a process for preparing the compound of the formula (I) in the polymorph I, by effecting the compound of the formula (I) in the polymorph II, obtained as described in example 1, in an inert solvent until the desired degree of conversion is attained, preferably until quantitative conversion to the polymorph I. If appropriate, crystals of the compound of the formula (I) in the polymorph I are added. The resulting crystals are isolated and, to remove solvent present, dried to constant weight at room temperature or at elevated temperature, for example from 40 to 80° C. The compound of the formula (I) is thus obtained in the polymorph I.
Effecting the compound of the formula (I) in the polymorph II in an inert solvent means, that for example the compound of the formula (I) in the polymorph II is dissolved completely (solution) or only in part (suspension). The mixture can be, for example stirred or shaken.
Suitable inert solvents are lower alcohols, for example methanol, ethanol, n-propanol, isopropanol, n-butanol, sec-butanol, isobutanol, or ketones such as acetone, or alkanes such as n-pentane, cyclopentane, n-hexane, cyclohexane, or tetrahydrofuran, or acetonitrile, or toluene, or ethyl acetate, or mixtures of the solvents mentioned, or mixtures of the solvents mentioned with water. Preference is given to methanol, ethanol, n-propanol, isopropanol, acetone, tetrahydrofuran, acetonitrile, toluene, ethyl acetate, mixtures of the solvents mentioned or mixtures of the solvents mentioned with water. Isopropanol, ethylacetate or a mixture thereof are used most preferably as inert solvents.
Preference is given to preparing the compound of the formula (I) in the polymorph I by effecting the compound of the formula (I) in the polymorph II, obtained as described in example 1, in methanol, ethanol, a mixture of both solvents or a mixture of both solvents with water, preferably a 1:1 mixture with water, and shaking or stirring at a temperature of from 50° C. up to the reflux temperature of the solvent, preferably at from 60 to 80° C., in the absence of crystals of a solvate of the compound of the formula (I), for example in the absence of crystals of the methanol solvate or the ethanol solvate of the compound of formula (I), for up to one day. The crystals are cooled to from −30° C. to room temperature, preferably from −25 to 10° C., isolated and dried. The compound of the formula (I) is thus obtained in the polymorph I. Most preferably isopropanol, ethylacetate or a mixture thereof is used as solvent.
Preference is likewise given to preparing the compound of the formula (I) in the polymorph I by effecting the compound of the formula (I) in the polymorph II, obtained as described in example 1, in methanol, ethanol, a mixture of both solvents or a mixture of both solvents with water, and shaking or stirring at a temperature of from 10° C. up to the reflux temperature of the solvent, preferably at room temperature, for up to 1 day. The mixture is subsequently seeded with crystals of the compound of the formula (I) in the polymorph I and stirred or shaken, for example at room temperature, for from 1 hour to 14 days, preferably from 2 hours to 7 days. The crystals are isolated and dried. The compound of the formula (I) is thus obtained in the polymorph I. Most preferably isopropanol, ethylacetate or a mixture thereof is used as solvent.
Preference is likewise given to preparing the compound of the formula (I) in the polymorph I by effecting the compound of the formula (I) in the polymorph II, obtained as described in example 1, in an inert solvent apart from methanol and/or ethanol, preferably isopropanol, acetone, tetrahydrofuran, acetonitrile, ethyl acetate, toluene, or a mixture thereof and stirring or shaking at a temperature of from 10° C. up to the reflux temperature of the solvent, preferably at from room temperature to 90° C., for up to 2 weeks, preferably from 1 day up to one week. If appropriate, the mixture is cooled to room temperature and the crystals are isolated and dried. The compound of the formula (I) is thus obtained in the polymorph I. Most preferably isopropanol, ethylacetate or a mixture thereof is used as solvent.
The compound of the formula (I) may likewise be prepared in the polymorph I by heating the compound of the formula (I) in the polymorph II to from 195 to 222° C., preferably from 195 to 215° C., for example at a heating rate of from 10° C. to 30° C. per minute, preferably from 15° C. to 25° C. per minute, and subsequently cooling to from 10° C. to 30° C., preferably to room temperature, for example at a cooling rate of from 1° C. to 4° C. per minute, preferably from 1° C. to 3° C. per minute.
The compound of the formula (I) in the polymorph III can be prepared by effecting the compound of the formula (I) in the polymorph II in an inert solvent, for example methanol. Filtration is effected after from 1 day to 1 week, and the product is dried and heat-treated at from 145 to 160° C. for from 15 minutes to 1 hour. The compound of the formula (I) is thus obtained in the polymorph III.
The methanol solvate of the compound of the formula (I) can be prepared by effecting the compound of the formula (I) in the polymorph II in methanol. After 1 week, filtration is effected, and the product is dried and stored under a methanol atmosphere for from 5 hours to 1 week. The methanol solvate of the compound of the formula (I) is thus obtained with a methanol content of 4.8% by weight.
The ethanol solvate of the compound of the formula (I) can be prepared by effecting the compound of the formula (I) in the polymorph II in ethanol. After 1 week, filtration is effected and the product is dried. The ethanol solvate of the compound of the formula (I) is thus obtained with an ethanol factor of 6.7 percent by weight.
The processes are generally carried out at atmospheric pressure. However, it is also possible to work at elevated pressure or at reduced pressure (for example in a range of from 0.5 to 5 bar).
The weight data in the tests and examples which follow are, unless stated otherwise, percentages by weight; parts are parts by weight. Solvent ratios, dilution ratios and concentration data of liquid/liquid solutions are based on each case on the volume.



WORKING EXAMPLES
The thermograms were obtained using a DSC 7 or Pyris-1 differential scanning calorimeter and TGA 7 thermogravimetric analyzer from Perkin-Elmer. The X-ray diffractograms were registered in a Stoe transmission diffractometer. The IR, FIR, NIR and Raman spectra were recorded using IFS 66v (IR, FIR), IFS 28/N (NIR) and RFS 100 (Raman) Fourier spectrometers from Bruker.
Example 1
4-{4-[({[4-Chloro-3-(trifluoromethyl)phenyl]amino}carbonyl)amino]phenoxy}-N-methylpyridine-2-carboxamide tosylate in the polymorph II
903 g of 4-{4-[({[4-chloro-3-(trifluoromethyl)phenyl]amino}carbonyl)amino]phenoxy}-N-methyl-pyridine-2-carboxamide, prepared as described in WO 00/42012, are initially charged in 2700 ml of ethanol. 451.7 g of p-toluenesulfonic acid monohydrate are dissolved in 1340 g of ethanol and added dropwise at room temperature. The suspension is stirred at room temperature for 1 hour, then filtered off with suction, and the residue is washed three times with 830 ml each time of ethanol. The drying is effected at 50° C. under reduced pressure with supply of air. 1129.6 g of the title compound in the polymorph II are obtained.
Example 2
Preparation of 4-{4-[({[4-chloro-3-(trifluoromethyl)phenyl]amino}carbonyl)-amino]phenoxy}-N-methylpyridine-2-carboxamide tosylate in the polymorph I
Example 2.1
5 mg of the tosylate salt of 4-{4-[({[4-chloro-3-(trifluoromethyl)phenyl]amino}carbonyl)amino]-phenoxy}-N-methylpyridine-2-carboxamide in the polymorph II are heated to 200° C. at a heating rate of 20° C./min and subsequently cooled to room temperature at a cooling rate of 2° C./min. The sample is tested thermoanalytically (DSC) and corresponds to the title compound in the polymorph I.
Example 2.2
75 mg of the tosylate salt of 4-{4-[({[4-chloro-3-(trifluoromethyl)phenyl]amino}carbonyl)-amino]phenoxy}-N-methylpyridine-2-carboxamide in polymorph II are dissolved in 10 ml of ethanol/water (1:1) at approximately 80° C. and filtered. The mixture is divided into two samples and sample A is crystallized in a refrigerator at +8° C. and sample B in a freezer at −20° C. After vaporization of the solvent mixtures, the two crystals of sample A and B are tested thermoanalytically (DSC). Both samples correspond to the title compound in the polymorph I.
Example 2.3
In each case 400 mg of the tosylate salt of 4-{4-[({[4-chloro-3-(trifluoromethyl)phenyl]-amino}carbonyl)amino]phenoxy}-N-methylpyridine-2-carboxamide in the polymorph II are suspended in a) 8 ml of methanol and b) in 8 ml of ethanol and each stirred at room temperature for 2 hours. The suspensions are each seeded with 2 mg of the title compound in the polymorph I and subsequently stirred at room temperature for 1 week. After filtration, the solid residues of the two samples are dried at room temperature. The residues are each tested thermoanalytically (DSC) and correspond to the title compound in the polymorph I.
Example 2.4
200 mg of the tosylate salt of 4-{4-[({[4-chloro-3-(trifluoromethyl)phenyl]amino}carbonyl)-amino]phenoxy}-N-methylpyridine-2-carboxamide in the polymorph II are suspended in 5 ml of ethanol/water (1:1 v/v) and stirred at room temperature for 2 hours. The suspension is seeded with 2 mg of the title compound in the polymorph I and subsequently stirred at room temperature for 1 week. After filtration, the solid residue is dried at room temperature. The residue is tested thermoanalytically (DSC) and corresponds to the title compound in the polymorph I.
Example 2.5
In each case 50 mg of the tosylate salt of 4-{4-[({[4-chloro-3-(trifluoromethyl)phenyl]amino}-carbonyl)amino]phenoxy}-N-methylpyridine-2-carboxamide in the polymorph II are admixed with in each case 2 ml of a) isopropanol, b) acetone, c) tetrahydrofuran, d) acetonitrile, e) ethyl acetate and f) toluene, and in each case stirred at room temperature for 6 days. In the case of c) tetrahydrofuran and f) toluene another 1 ml of the particular solvent is added. The suspensions are each filtered and the particular residues are dried at room temperature. The residues are each tested by X-ray diffractometry and correspond to the title compound in the polymorph I.
Example 2.6
200 mg of the tosylate salt of 4-{4-[({[4-chloro-3-(trifluoromethyl)phenyl]amino}carbonyl)-amino]phenoxy}-N-methylpyridine-2-carboxamide in the polymorph II are suspended in 4 ml of toluene and the suspension is stirred at 80° C. for one week. After cooling to room temperature, the residue is filtered, dried at room temperature and tested by X-ray diffractometry. The title compound is obtained in the polymorph I.
Example 3
Preparation of 4-{4-[({[4-chloro-3-(trifluoromethyl)phenyl]amino}carbonyl)-amino]phenoxy}-N-methylpyridine-2-carboxamide tosylate in the polymorph III
3.5 g of the tosylate salt of 4-{4-[({[4-chloro-3-(trifluoromethyl)phenyl]amino}carbonyl)-amino]phenoxy}-N-methylpyridine-2-carboxamide in the polymorph II are suspended in 15 ml of methanol and stirred at room temperature. After one week, the suspension is filtered and the residue dried at room temperature. Subsequently, the product is heat-treated at 150° C. for 30 min. The residue is analyzed by X-ray diffractometry and corresponds to the title compound in the polymorph III.
Example 4
Preparation of the methanol solvate of 4-{4-[({[4-chloro-3-(trifluoromethyl)phenyl]amino}carbonyl)amino]phenoxy}-N-methylpyridine-2-carboxamide tosylate
3.5 g of the tosylate salt of 4-{4-[({[4-chloro-3-(trifluoromethyl)phenyl]amino}carbonyl)amino]-phenoxy}-N-methylpyridine-2-carboxamide in the polymorph II are suspended in 15 ml of methanol and stirred at room temperature. After one week, the suspension is filtered and the residue is dried at room temperature. Subsequently, the product is stored in a desiccator with a methanol atmosphere for one day. The residue is analyzed by X-ray diffractometry and corresponds to the methanol solvate of the title compound with a methanol content of 4.8 percent by weight.
Example 5
Preparation of the ethanol solvate of 4-{4-[({[4-chloro-3-(trifluoromethyl)phenyl]amino}carbonyl)amino]phenoxy}-N-methylpyridine-2-carboxamide tosylate
3 g of the tosylate salt of 4-{4-[({[4-chloro-3-(trifluoromethyl)phenyl]amino}carbonyl)amino]-phenoxy}-N-methylpyridine-2-carboxamide in the polymorph II are suspended in 15 ml of ethanol and stirred at room temperature. After one week, the suspension is filtered and the residue is dried at room temperature. The residue is analyzed by X-ray diffractometry and corresponds to the ethanol solvate of the title compound with an ethanol content of 6.7 percent by weight.







TABLE 1







Differential Scanning Calorimetry and Thermogravimetry













Polymorph
Polymorph
Polymorph
Methanol-
Ethanol



I
II
III
solvate
solvate
















Melting
223-231*
194**
187-190
—
—


point


[° C.]


Loss in
<0.5 
<0.5
<0.5
4.8
6.7


mass


[%


by wt.]





*= melting under decomposition


**= Conversion point













TABLE 2







X-ray diffractometry


Reflections












Polymorph
Polymorph
Methanol
Ethanol


Polymorph I
II
III
solvate
solvate


[2 theta]
[2 theta]
[2 theta]
[2 theta]
[2 theta]














4.4
7.3
7.7
8.0
7.9


10.7
8.8
8.5
8.4
8.4


11.1
10.5
9.8
9.3
9.3


11.4
12.4
10.6
11.2
9.5


11.6
12.8
12.0
12.2
11.2


12.2
13.1
12.3
13.0
12.0


12.8
13.4
12.9
13.4
12.2


13.2
13.6
13.4
15.8
12.8


14.8
14.0
13.5
16.3
13.4


16.5
14.7
15.4
16.9
15.9


16.7
15.5
16.0
17.7
16.1


17.7
15.7
16.5
18.3
16.8


17.9
15.9
16.9
18.7
17.4


18.8
16.4
17.3
19.0
17.7


19.3
17.0
17.8
19.4
18.1


19.6
17.6
18.7
20.2
18.3


20.1
17.9
18.8
20.5
18.6


20.5
18.3
19.3
20.9
18.8


20.8
19.3
19.9
21.4
19.4


21.5
20.2
20.3
21.7
20.0


21.7
20.8
20.8
22.3
20.4


22.3
21.1
21.2
22.4
21.0


22.5
21.9
21.6
23.8
21.2


22.9
22.6
22.5
24.0
21.5


23.4
22.8
23.0
24.4
21.7


23.7
23.2
23.4
24.7
22.3


24.0
24.0
24.2
24.9
22.4


24.5
24.6
24.5
25.2
22.8


25.1
25.4
24.8
25.7
23.3


25.4
25.9
25.2
26.0
23.6


26.0
26.7
25.9
26.1
23.8


26.4
27.1
26.9
26.4
24.3


26.6
28.2
27.5
26.9
24.7


27.0
28.4
27.7
27.0
25.3


27.6
29.7
28.2
27.5
25.8


28.2
30.7
29.2
27.7
25.9


28.6
31.4
29.4
28.1
26.4


28.8
32.5
29.8
28.3
26.9


29.3
33.4
30.3
28.8
27.3


29.6
34.7
31.4
29.1
27.6


29.9
35.0
32.2
29.7
28.3


30.8
35.9
33.5
30.2
28.8


31.2
36.5
34.0
30.4
29.1


31.6

35.2
30.7
29.5


31.8

36.1
30.8
29.7


32.1

37.2
31.4
30.2


32.4

37.7
31.6
30.4


32.7


31.9
30.9


33.1


32.3
31.4


33.8


32.6
32.0


34.2


32.9
32.6


34.6


33.4
32.9


35.4


33.8
33.2


35.7


34.0
33.7


37.1


34.2
33.9





34.5
34.5





34.9
35.5





36.2
36.0





36.6
36.3





37.2
36.6





37.7
37.1






37.7
















TABLE 3







IR spectroscopy


Peak maxima












Polymorph
Polymorph
Methanol
Ethanol


Polymorph I
II
III
solvate
solvate


[cm−1]
[cm−1]
[cm−1]
[cm−1]
[cm−1]














3390
3345
3374
3317
3313


3289
3277
3280
3098
3237


3256
3108
3250
3072
3067


3212
3073
3081
2948
2980


3144
2944
2955
2836
2895


3113
1908
1917
1712
1710


3080
1718
1715
1692
1692


2943
1696
1691
1632
1633


1908
1632
1632
1608
1607


1724
1597
1604
1546
1545


1689
1547
1551
1506
1505


1630
1521
1539
1483
1483


1611
1506
1526
1464
1465


1598
1486
1503
1406
1406


1558
1468
1484
1337
1337


1529
1403
1461
1314
1314


1506
1333
1418
1287
1286


1485
1307
1404
1258
1258


1458
1287
1385
1232
1234


1419
1255
1337
1193
1191


1401
1237
1308
1170
1171


1327
1206
1284
1140
1140


1310
1189
1258
1128
1115


1281
1163
1234
1115
1044


1256
1135
1209
1030
1035


1239
1125
1189
1017
1005


1220
1116
1178
1008
947


1189
1031
1140
947
925


1182
1007
1127
925
896


1132
944
1118
904
879


1117
920
1034
877
861


1032
900
1010
848
849


1009
880
947
831
830


950
843
920
822
823


939
827
900
779
778


922
813
877
744
744


879
787
842
720
721


846
779
834
711
711


822
745
825
683
682


777
720
818
663
663


748
709
776
617
592


721
684
744
591
570


711
660
721
571
562


681
568
710
561
533


662
552
683
534
510


565
544
663
509


553
505
572
488


533

564
468


513

548
442




509




484




469




443
















TABLE 4







Raman spectroscopy


Peak maxima












Polymorph
Polymorph
Methanol
Ethanol


Polymorph I
II
III
solvate
solvate


[cm−1]
[cm−1]
[cm−1]
[cm−1]
[cm−1]














3113
3115
3107
3098
3094


3100
3095
3069
3066
3066


3068
3064
3016
2929
3046


2986
3052
2958
2838
2977


2951
2924
2930
1711
2927


2925
2812
2883
1688
2884


2817
1719
2814
1608
2812


2577
1696
2741
1548
1710


1723
1609
1715
1507
1690


1689
1604
1690
1406
1635


1613
1550
1632
1386
1607


1606
1521
1605
1334
1547


1556
1507
1567
1313
1506


1529
1414
1549
1299
1461


1506
1402
1504
1266
1406


1442
1376
1475
1235
1385


1419
1334
1448
1213
1334


1401
1312
1413
1193
1313


1369
1268
1404
1172
1299


1328
1255
1386
1114
1288


1310
1234
1334
1033
1264


1278
1207
1310
1004
1235


1267
1161
1284
925
1213


1245
1136
1265
863
1194


1212
1116
1236
819
1168


1186
1103
1215
802
1141


1163
1031
1180
786
1113


1134
1009
1161
746
1033


1117
944
1120
721
1017


1032
922
1106
684
1003


1010
858
1034
663
925


922
825
1009
638
891


880
813
926
618
862


862
798
860
555
850


824
787
815
492
819


802
746
801
442
802


789
718
785
397
786


749
684
746
372
779


722
662
716
359
746


692
636
683
339
720


683
568
663
310
694


663
553
637
297
684


637

619
235
663


552

553
176
638


492

510
130
616


471

461
109
590


445

443

553


436

418

512


423



491


399



459


390



442


367



396


313



387


305



373


292



362


231



353






337






313






306






295






237






217






181






109
















TABLE 5







FIR spectroscopy


Peak maxima












Polymorph
Polymorph
Methanol
Ethanol


Polymorph I
II
III
solvate
solvate


[cm−1]
[cm−1]
[cm−1]
[cm−1]
[cm−1]














495
496
484
489
490


470
468
468
468
469


458
457
443
460
459


445
443
432
442
441


436
429
423
433
433


423
413
417
425
426


408
400
396
397
396


399
386
381
386
387


368
366
370
372
373


356
345
358
358
353


321
321
351
351
337


304
304
335
338
318


291
293
309
316
294


252
255
301
296
280


238
237
290
280
266


210
213
255
252
252


173
190
241
236
237


120
175
224
216
216


114
156
210
178
180



137
177
146
166



106
147
114
152




108
104
143





99
116






106






97






93
















TABLE 6







NIR spectroscopy


Peak maxima












Polymorph
Polymorph
Methanol
Ethanol


Polymorph I
II
III
solvate
solvate


[cm−1]
[cm−1]
[cm−1]
[cm−1]
[cm−1]





8820
8791
8843
8829
8828


8407
8395
8420
8424
8442


8186
8167
8200
7191
7191


7182
7122
7155
6421
6631


6934
6111
6658
6081
6422


6664
6017
6510
6024
6073


6494
5974
6432
5964
6022


6087
5914
6108
5896
5962


6030
5789
6023
5555
5891


5988
5746
5891
5288
5785


5934
5641
5793
4908
5287


5881
5555
5739
4661
4908


5747
5501
5652
4606
4659


5648
5339
5262
4574
4605


5338
5219
4982
4404
4572


4984
4895
4919
4329
4421


4914
4789
4847
4278
4346


4791
4661
4788
4207
4259


4691
4606
4708
4174
4202


4573
4563
4666
4080
4170


4399
4512
4571
4057
4096


4312
4403
4409

4080


4275
4275
4344

4051


4208
4226
4305


4088
4155
4282



4095
4227



4064
4200




4091




4063

## Claims

1. A compound of the formula (I)



in the polymorph I form, which shows in the X-ray diffractometry peak maxima of the 2 theta angle of 4.4, 14.8 and 20.5.

2. The compound of claim 1 which shows in the X-ray diffractometry peak maxima of the 2 theta angle comprising 4.4, 14.8, 20.5, 20.8, 21.5 and 22.9.

3. The compound of claim 1 which shows in the IR spectrum a peak maximum of 1724 cm−1.

4. The compound of claim 1 which shows in the Raman spectrum a peak maximum of 1723 cm−1.

5. A method of preparing the compound of formula (I)



in the polymorph I form which shows in the X-ray diffractometry peak maxima of the 2 theta angle of 4.4, 14.8 and 20.5, comprising contacting a compound of formula (I) in the polymorph II form with an inert solvent under conditions sufficient to quantitatively convert the compound in the polymorph II form to the polymorph I form.

6. The method according to claim 5, further comprising the step of seeding the inert solvent with crystals of a compound of the formula (I) in the polymorph I form.

7. A method of preparing a compound of formula (I)



in the polymorph I form which shows in the X-ray diffractometry peak maxima of the 2 theta angle of 4.4, 14.8 and 20.5, comprising heating a compound of formula (I) in the polymorph II form from 195° C. to 222° C. at a heating rate of 10° C. to 30° C. per minute and subsequently cooling to 10° C. to 30° C. at a cooling rate of from 1 to 4° C. per minute.

8. A pharmaceutical composition comprising a compound of formula (I):



substantially in the polymorph I form which shows in the X-ray diffractometry peak maxima of the 2 theta angle of 4.4, 14.8 and 20.5.

9. The pharmaceutical composition as claimed in claim 8, further comprising one or more inert, nontoxic, pharmaceutically suitable excipients.

10. The pharmaceutical composition of claim 8, wherein the compound of formula (I) is present in the polymorph I form in the composition in an amount equal to or more than 90 percent by weight of the total weight of the compound of formula (I) present in the composition.

11. The pharmaceutical composition according to claim 8, further comprising another pharmaceutical agent where the combination causes no unacceptable side effects.

12. The pharmaceutical composition of claim 8, further comprising another pharmaceutical agent which is a cytotoxic agent, a signal transduction inhibitor, an anti-cancer agent, or an antiemetic.

13. A pharmaceutical composition comprising a compound of formula (I):



substantially in the polymorph I form which shows in the X-ray diffractometry peak maxima of the 2 theta angle of 4.4, 14.8 and 20.5, and at least one additional pharmaceutical agent.

14. The pharmaceutical composition of claim 13, further comprising one or more inert, nontoxic, pharmaceutically suitable excipients.

15. The pharmaceutical composition of claim 13, wherein said additional pharmaceutical agent is a cytotoxic agent, a signal transduction inhibitor, an anti-cancer agent, or an antiemetic.

16. A method of treating a disorder, comprising administering to a subject in need thereof a therapeutically effective amount of a compound of formula (I)



in the polymorph I form which shows in the X-ray diffractometry peak maxima of the 2 theta angle of 4.4, 14.8 and 20.5.

17. The method of claim 16, wherein the disorder is selected from the group consisting of abnormal angiogenesis, hyperpermeability processes, bone marrow diseases, carcinoma and carcinogenic cell growth.

18. The method of claim 16, wherein the disorder is leukemia, or carcinoma of the lung, pancreas, thyroid gland, kidney or intestine.

19. A method for treating a disorder, comprising administering to a subject in need thereof a therapeutically effective amount of the pharmaceutical composition of any one of claims 8 to 15.

20. The method of claim 19, wherein the disorder is selected from the group consisting of abnormal angiogenesis, hyperpermeability processes, bone marrow diseases, carcinoma and carcinogenic cell growth.

21. The method of claim 19, wherein the disorder is leukemia, or carcinoma of the lung, pancreas, thyroid gland, kidney or intestine.

22. A method of preparing a compound of formula (I)



in the polymorph I form which shows in the X-ray diffractometry peak maxima of the 2 theta angle of 4.4, 14.8 and 20.5, comprising dissolving or suspending a compound of formula (I) in the polymorph II form in an inert solvent and stirring or shaking under conditions sufficient to quantitatively convert the compound in the polymorph II form to the polymorph I form.

23. The compound of the formula (I) as claimed in claim 22, wherein the process for its preparation further comprises the step of seeding the inert solvent with crystals of a compound of the formula (I) in the polymorph I form.

24. The compound of claim 1 prepared by dissolving or suspending a compound of formula (I) in the polymorph II form in an inert solvent and stiffing or shaking under conditions sufficient to quantitatively convert the compound in the polymorph II form to the polymorph I form.

25. The compound of claim 1 prepared by dissolving or suspending a compound of formula (I) in the polymorph II form in an inert solvent, seeding the inert solvent with crystals of a compound of the formula (I) in the polymorph I form and stiffing or shaking under conditions sufficient to quantitatively convert the compound in the polymorph II form to the polymorph I form.

26. The compound of claim 1 prepared by a method comprising heating a compound of formula (I) in the polymorph II form from 195° C. to 222° C. at a heating rate of 10° C. to 30° C. per minute and subsequently cooling to 10° C. to 30° C. at a cooling rate of from 1 to 4° C. per minute to quantitatively convert the compound in the polymorph II form to the polymorph I form.

27. The compound of claim 1 which shows in the X-ray diffractometry peak maxima of the 2 theta angle of 4.4, 14.8 and 20.5, which shows in the IR spectrum a peak maximum of 1724 cm−1 and which shows in the Raman spectrum a peak maximum of 1723 cm−1.

28. A compound having the x-ray diffraction pattern of polymorph I in FIG. 2 of the application which shows in the X-ray diffractometry peak maxima of the 2 theta angle of 4.4, 14.8 and 20.5.

29. The compound of claim 1 which shows in the X-ray diffractometry peak maxima of the 2 theta angle comprising: 4.4, 13.2, 14.8, 16.7, 17.9, 20.1, 20.5, 20.8, 21.5 and 22.9.

30. The compound of claim 1 which shows in the X-ray diffractometry peak maxima of the 2 theta angle comprising: 4.4, 10.7, 11.1, 11.4, 11.6, 12.2, 12.8, 13.2, 14.8, 16.5, 16.7, 17.7, 17.9, 18.8, 19.3, 19.6, 20.1, 20.5, 20.8, 21.5, 21.7, 22.3, 22.5, 22.9, 23.4, 23.7, 24.0, 24.5, 25.1, 25.4, 26.0, 26.4, 26.6, 27.0, 27.6, 28.2, 28.6, 28.8, 29.3, 29.6, 29.9, 30.8, 31.2, 31.6, 31.8, 32.1, 32.4, 32.7, 33.1, 33.8, 34.2, 34.6, 35.4, 35.7 and 37.1.

31. The compound of claim 1 which melts under decomposition at 223° C.-231° C.