# Patent

## Title
Method of reducing the viscosity of mucus

## Abstract

Disclosed is a pO157 plasmid-specified polypeptide found in E. coli EDL933 and other E. coli that binds to and cleaves C1-esterase inhibitor, and antibodies specific for the polypeptide. Also disclosed are methods employing the polypeptide for diagnosing enterohemorrhagic E. coli infection, identifying potential inhibitors of its activity, and reducing viscosity of material containing glycosylated polypeptides.

## Background
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a continuation of U.S. application Ser. No. 11/294,087, filed Dec. 5, 2005, now U.S. Pat. No. 7,264,922 which is a continuation-in-part of U.S. application Ser. No. 10/786,445, filed Feb. 25, 2004, now U.S. Pat. No. 7,214,479, which is a continuation-in-part of U.S. application Ser. No. 10/002,309, filed Oct. 26, 2001, now U.S. Pat. No. 6,872,559, which claims priority to U.S. Provisional Application No. 60/243,675, filed Oct. 26, 2000. U.S. application Ser. No. 11/294,087, filed Dec. 5, 2005 also claims priority to U.S. Provisional Application No. 60/651,560, filed Feb. 10, 2005 and U.S. Provisional Application No. 60/633,583, filed Dec. 6, 2004.


STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT
This invention was made with United States government support awarded by NIH AI051735.


INTRODUCTION
Enterohemorrhagic Escherichia coli (EHEC) serotype O157:H7 strain is a human enteric bacterial pathogen that causes diarrheal disease, hemorrhagic colitis, and hemolytic uremic syndrome (HUS). Each year in the United States, an estimated 20,000 people suffer from diarrheal disease associated with E. coli O157:H7 infection, which is typically contracted by ingesting contaminated foods, especially undercooked meat. Approximately 6% of infected individuals develop HUS, which can lead to renal failure and death. Young children and the elderly are particularly susceptible to developing HUS.
In general, bacterial infections are commonly treated by administering appropriate antibiotics. However, E. coli O157:H7 infection typically has a very rapid progression, and is consequently very difficult to treat. Often by the time the disease is diagnosed, the infected individual is severely ill and toxic proteins secreted by the bacteria may have damaged mucosal cells and entered the blood stream. Antibiotic treatment of patients infected with E. coli O157:H7 is generally not successful and, in fact, is believed to be contraindicated.
E. Coli O157:H7 bacteria are very proficient at establishing an infection; ingestion of as few as 10 live bacteria is sufficient to establish an infection. The highly infective nature of E. coli O157:H7 and the devastating sequelae associated with infection by this bacteria, together with the extensive public attention given to outbreaks of hemorrhagic colitis, has generated a great deal of interest among medical professionals and the general public in developing the means for early diagnosis and treatment of the disease. The entire genome of the E. coli O157:H7 EDL933W (ATCC 43895) was sequenced with the expectation that valuable information concerning the organism's pathogenicity would be uncovered, which may facilitate development of methods of preventing infections, or preventing or treating hemolytic uremic syndrome in individuals infected with of the organism. The DNA sequence of E. coli O157:H7 was compared with that of E. coli K12, a non-pathogenic strain commonly used in research. The genome of E. coli O157:H7 exceeds that of E. coli K-12 by more than a million base pairs and has up to 1000 genes not found on K-12. These additional gene sequences are distributed throughout more than 250 sites in islands, with each island containing from zero to sixty genes (1).
There is a need for improved methods of early detection of E. coli O157:H7 infections and for methods of preventing or treating individuals infected with E. coli O157:H7.
BRIEF SUMMARY OF THE INVENTION
In one aspect, the present invention provides a purified antibody that binds specifically to a polypeptide comprising SEQ ID NO:2.
In another aspect, the present invention includes a method of reducing colonization of epithelial cells by StcE producing bacteria comprising contacting the epithelial cells with an antibody that binds specifically to SEQ ID NO:2 or an inhibitor of StcE.
In yet another aspect, the invention provides a composition comprising a purified polypeptide comprising at least 25 consecutive amino acid residues of SEQ ID NO:2 and an adjuvant.
Also provided is a method of eliciting an immune response in an animal comprising inoculating the animal with a composition comprising a purified polypeptide comprising at least 25 consecutive amino acid residues of SEQ ID NO:2 and an adjuvant.
The present invention also provides a method of reducing complement-mediated disruption of cells comprising contacting the cells with a purified polypeptide comprising amino acid residues 24-886 of SEQ ID NO:2 or SEQ ID NO:19 so as to reduce complement-mediated disruption relative to that of untreated cells.
The invention further provides a method of reducing the viscosity of a material comprising a mucin or a glycosylated polypeptide comprising contacting the material with a viscosity reducing effective amount of StcE.
In another aspect, the invention provides a composition for enhancing delivery of a target antigen to mucosal cells comprising the target antigen and StcE.
The composition for enhancing delivery of a target antigen to mucosal cells may be used in a method of eliciting in an animal an immune response to a target antigen comprising contacting the mucosal cells of the animal with the composition.
The present invention provides a method of detecting StcE in a sample by detecting binding of an antibody with selectively for a polypeptide comprising SEQ ID NO:2 of claim 1 to a polypeptide in the sample.
In another aspect, the invention includes detecting StcE activity by contacting the sample with C1-INH under suitable conditions to allow cleavage of C1-INH by StcE, if present and detecting C1-INH cleavage.
The invention also provides a method of evaluating a test substance for the ability to inhibit StcE comprising contacting C1-INH with the test substance and StcE and detecting C1-INH cleavage.



BRIEF DESCRIPTION OF THE SEVERAL VIEWS OF THE DRAWINGS
FIG. 1 shows the differential effect of E. coli strains containing (FIG. 1A and FIG. 1C) or lacking (FIG. 1B and FIG. 1D) the plasmid pO157 on aggregation of T cells.
FIG. 2 shows stained proteins separated by SDS/PAGE.
FIG. 3 shows that synthesis of StcE correlates with the presence of pO157.
FIG. 4 shows that C1 inhibitor in human serum is cleaved by StcE.
FIG. 5 shows cleavage of C1 inhibitor over time.
FIG. 6 shows differential cleavage of C1-INH by StcE and elastase.
FIG. 7 is an immunoblot showing a StcE-reactive band in fecal filtrates.
FIG. 8A shows that StcE E435D-His is unable to cleave C1-INH; FIG. 8B shows that StcE E435D-His is unable to bind C1-INH.
FIG. 9 shows amplification of three stcE-specific sequences from all E. coli isolates containing the pO157, and amplification of two of the three stcE-specific sequences.
FIG. 10 shows an immunoblot of culture supernatants probed with a polyclonal antibody to StcE.
FIG. 11 shows an immunoblot of C1-INH following incubation with culture supernatants, probed with anti-C1-INH antibody.
FIG. 12 shows the percent lysis of erythrocytes in classical complement-mediated erythrocyte lysis by human serum as a function of StcE (solid squares) or BSA (open squares) concentration.
FIG. 13A shows the percent lysis of erythrocytes contacted with C1-INH treated with increasing concentrations of StcE′-His (closed squares) or BSA (open squares) prior to adding human serum. The point indicated by a circle lacked C1-INH. FIG. 13B shows the percent lysis of erythrocytes contacted with C1-INH treated StcE′-His (closed squares) or BSA (open squares) prior to adding human as a function of C1-INH concentration.
FIG. 14A shows binding of StcE′-His or Alexa-StcE′-His to erythrocytes as detected by flow cytometry. FIG. 14B shows mean fluorescence of erythrocytes detected by flow cytometry as a function of increasing concentrations of Alexa-StcE′-His.
FIG. 15A shows detection of erythrocyte binding by untreated C1-INH, or C1-INH treated with buffer alone (“mock treated”) or StcE′-His as detected by flow cytometry. FIG. 15B shows detection of erythrocyte binding by C1-INH treated with StcE′-His, with or without subsequent removal of StcE′-His, as detected by flow cytometry. FIG. 15C shows mean fluorescence detection of erythrocyte binding by C1-INH treated with StcE′ E435D-His with increasing concentrations of C1-INH. FIG. 15D shows a blot of immunoprecipitated C1-INH untreated or treated with StcE′-His or StcE′ E435D-His, separated by SDS-PAGE, transferred to nitrocellulose, and probed with an anti-StcE′ Ab.
FIG. 16A shows the percent lysis of erythrocytes in classical complement-mediated erythrocyte lysis by human serum following pretreatment with C1-INH, C1-INH and StcE′-His, or C1-INH and StcE′ E435D-His. FIG. 16B shows binding of C1-INH was untreated or treated with StcE′-His or StcE′ E435D-His before the addition of sheep erythrocytes as detected by flow cytometry.
FIG. 17A shows the relative kallikrein activity in the presence of increasing concentrations of C1-INH in the presence or absence of StcE′-His. FIG. 17B shows an immunoblot of C1s untreated or treated with C1-INH in the absence or presence of StcE′-His or StcE′ E435D-His.
FIG. 18A shows blots of C1-INH untreated or treated with StcE′-His or kallikrein and probed with a polyclonal anti-human C1-INH Ab (left panel), mAb 3C7 (middle panel), or mAb 4C3 (right panel). FIG. 17B shows electrophoretically separated 35S-methionine-labeled full length hC1-INH or C-serp(98), a recombinant C1-INH molecule truncated at amino acid 98, each untreated or treated with StcE′-His and immunoprecipitated with polyclonal anti-human C1-INH IgG-Protein A sepharose.
FIG. 19 shows the percent survival of serum sensitive bacteria in the presence of serum and of C1-INH, StcE′-His, C1-INH and StcE′-His, or no additional protein.
FIG. 20 compares the number of pedestals per field formed for wild type, StcE knockout, and complemented E. coli O157:H7 strains on HEp-2 cells in the presence or absence of exogenous StcE.
FIG. 21 compares the viscosity of saliva before (zero hour) and after incubation in buffer (squares) or in StcE (triangles) relative to water (dashed line) as measured by elution time as a function of incubation time.
FIG. 22 shows human salival proteins separated by SDS-PAGE and stained with Coomassie.
FIG. 23 is a graph showing the concentration of detectable C1-INH in a well as a function of the amount of C1-INH added to the well for samples treated with StcE in the presence (squares) or absence (triangles) of EDTA.



DETAILED DESCRIPTION OF THE INVENTION
Each of the publications or applications cited or listed herein is incorporated by reference in its entirety.
Strains of the serotype O157:H7 EDL933W contain a 92 kb plasmid designated pO157. As described in the Examples below, bacterial strains containing the plasmid cause the aggregation of two cultured human CD4+ T cell lines, Jurkat and MOLT-4, but do not cause aggregation of a B cell lymphoma line (Raji), or of macrophage-like cell lines (U937 and HL-60). Aggregation of the CD4+ T cells occurs in the presence of serum, but not in its absence. Strains lacking the plasmid do not cause aggregation of CD4+ T cells.
We employed transposon mutagenesis to identify a gene on pO157 of previously unknown function whose product is associated with the observed aggregation effect. The coding sequence and the deduced amino acid sequence of the protein it encodes are shown in SEQ ID NO:1 and SEQ ID NO:2, respectively. The protein, designated “StcE”, contains a putative cleavable N-terminal signal sequence. In cultures of bacteria expressing StcE, at least a portion of expressed StcE protein appears to be secreted into the culture medium, although StcE may also be associated with the cell surface.
A genetic construct expressing a His-tagged StcE protein was made by ligating the StcE coding sequence in frame to a sequence specifying a poly-His tail to permit expression and recovery of relatively large amounts of highly purified StcE protein, as described below.
In order to further evaluate the StcE protein for possible cytotoxic effects, variety of cell types were treated with StcE protein as described in the examples. Cells treated with StcE showed a high degree of aggregation in the presence of serum, but not in the absence of serum.
Because StcE-mediated aggregation occurred only in cells also treated with serum, the ability of StcE to bind to a specific serum protein was evaluated by Far Western blotting using the StcE protein as the probe. An acidic serum protein of about 105 kDa by SDS PAGE was identified as binding to StcE. The target protein was recovered, subjected to limited digestion by an endopeptidase, and the peptide products analyzed by mass spectrometry. The protein to which StcE binds was identified as C1-inhibitor (C1-INH), which serves as a critical inhibitor in the proteolytic cascade involved in complement activation.
The plasma protein C1-INH is a serpin (serine protease inhibitor) that controls the activation of C1, the first component of the complement cascade. The C1 component is made up of three subcomponents: C1q, C1r, and C1s. In the classical pathway of complement activation, C1 binds to an antigen-antibody complex or certain pathogens (e.g., HIV-1), which causes the proteolytic autoactivation of C1r, which in turn causes the proteolytic activation of C1s. C1-INH inhibits activation of the classical pathway by binding to C1 and inactivating C1r and C1s. In addition to its role in controlling activation of the classical complement pathway, C1-INH inhibits other serine proteases involved in the intrinsic coagulation pathway and kinin-forming system (reviewed in (3)).
Treatment of serum or purified C1-INH with purified StcE results in the apparent disappearance of C1-INH, presumably as a result of specific proteolytic cleavage of C1-INH by StcE. The predicted StcE amino acid sequence comprises the sequence HEVGHNYGLGH (SEQ ID NO:3) (residues 434-444 of SEQ ID NO:2), which corresponds to the histidine rich active site of metalloproteases (5). Further evidence that StcE may be a metalloprotease is provided by the observation that proteolysis of C1-INH by StcE is reduced in the presence of EDTA or BPS, which chelate divalent metal ions (e.g., Zn2+) required for metalloprotease activity.
In U.S. application Ser. No. 10/002,309, filed Oct. 26, 2001, of which the present application is a continuation-in-part, we proposed that the putative protease StcE by enterohemorrhagic strains of E. coli EHEC may lead to proteolysis of C1-INH and reduction of C1-INH activity. Loss of C1-INH activity may result in unregulated pro-inflammatory or coagulation response that may be responsible for tissue damage in the intestine and kidney of persons infected with EHEC. It is also possible that the StcE serum-dependent cellular aggregation phenotype plays a role in the pathogenesis of HUS because one of the hallmarks of HUS is thrombocytopenia with an accumulation of a large number of platelets in renal microthrombi. The kidneys of those diagnosed with HUS also contain large amounts of deposited fibrin.
Deficiencies in C1-INH can lead to a variety of diseases. For example, a hereditary deficiency in C1-INH (hereditary angiodema) is characterized by transient, recurrent attacks of intestinal cramps, vomiting and diarrhea. Hereditary defects in production of a different inhibitor of the complement cascade, Factor H, are associated with a form of hemolytic uremic syndrome (HUS) similar to that described for EHEC-mediated HUS.
The proteolytic activity of StcE may be a common mode of pathogenesis among some diarrheagenic strains of E. coli. Colony blot analysis and amplification of E. coli DNA using oligomers specific to the pO157 version of stcE indicate that the stcE gene is common to all tested strains of E. coli associated with bloody colitis and HUS, but the stcE gene is not present in enteroinvasive, enterotoxigenic or uropathogenic strains of E. coli. However, some closely related strains of enteropathogenic E. coli contain stcE, which suggests that StcE may be more widely distributed among diarrheagenic E. coli than appreciated initially. Additionally, a search of the GenBank database has identified at least one distant homolog to StcE: a Vibrio cholerae protein (designated TagA) of unknown function. We envision methods to screen for similar virulence factors produced by other microbes.
We discovered that in the presence of cells, StcE surprisingly potentiates the ability of C1-INH to reduce complement-mediated disruption of cells. Rather than reducing or destroying C1-INH serpin activity, the interaction between cell-associated StcE and C1-INH appears to enhance the ability of C1-INH to inhibit the classical complement cascade, thereby protecting cells from the lytic effects of complement activity. As detailed in the Examples, the addition of StcE-treated C1-INH to opsonized sheep erythrocytes and human serum significantly decreases erythrocyte lysis below that of equivalent amounts of native C1-INH alone. A decrease in complement activity when human serum was treated with StcE prior to the addition of sheep erythrocytes was also observed, which is likely due to the activity of StcE on the endogenous C1-INH found in serum. Furthermore, analysis of complement component deposition on erythrocyte surfaces indicates that StcE-treated C1-INH continues to act on its natural targets C1r and/or C1s.
StcE is unable to potentiate C1-INH activity in the absence of cells. Rather than directly modifying C1-INH to increase its ability to inhibit the complement cascade, StcE potentiation of C1-INH in the presence of cells may be due to tethering C1-INH to the cell surface, effectively increasing the local concentration of C1-INH at the sites of potential lytic complex formation.
Cleavage of C1-INH by StcE does not appear to be a factor in StcE potentiation of C1-INH protection against complement activity, as is evidenced by the ability of StcE E435D-His, a mutant protein defective in proteolytic activity against C1-INH, to protect erythrocytes to a degree comparable to that observed with wild-type StcE in vitro. The interaction between StcE and erythrocytes is specific, saturating the cells at approximately 1.8×106 molecules of StcE per cell. In turn, this allows a high affinity interaction between C1-INH and erythrocytes, reaching 2.25×106 molecules of C1-INH per cell in the presence of one μg of StcE. This amount of C1-INH (0.1 IU) is well within the physiological concentration of C1-INH found in serum, suggesting that this interaction may be biologically relevant in vivo.
Without being limited as to theory, we propose a model of the mechanism by which StcE potentiates C1-INH-mediated inhibition of classical complement in which StcE first interacts with the cell surface, followed by binding of StcE to the N-terminal domain of C1-INH to sequester the serpin to the cell surface. Alternatively, StcE may interact with C1-INH before binding to the cell surface. Cell-bound C1-INH binds to C1r and/or C1s of the C1 complex via the RCL, inactivating the serine protease. StcE then cleaves within the N-terminus of C1-INH, releasing the serpin/serine protease complex and a smaller, amino-terminal cleavage fragment of C1-INH from the cell surface. StcE binds to another C1-INH molecule, and the cycle described above is repeated.
The concept of C1-INH turnover at the cell surface may explain the observation that more C1-INH bound to erythrocytes in the presence of the proteolytically inactive StcE mutant than was bound in the presence of StcE′-His. Thus, the relatively slow cleavage rate of C1-INH by StcE may actually be beneficial to the potentiation of the serpin, as a more rapid rate of catalysis might not allow enough time for the inactivation of the C1 complex to occur. Additionally, the interaction of StcE with the N-terminal region of C1-INH while bound to the cell surface likely permits continued access of the serpin domain to its targets without compromising its activity. The binding of StcE to host cells might also allow the protease to be carried to sites distal to E. coli O157:H7 colonization in a manner similar to the Shiga toxin, thereby affecting C1-INH-regulated processes outside the local environment of bacterial infection. Finally, the observation that cell-bound C1-INH might affect leukocyte adhesion suggests StcE could influence the migration of inflammatory mediators to the sites of EHEC colonization.
Sequestration of complement inhibitors to bacterial surfaces can reduce complement activity and promote serum resistance. As demonstrated in the Examples, StcE-treated C1-INH acts in a similar by providing increased serum resistance to E. coli over native C1-INH. By preventing complement activity at such an early stage in the pathway via the recruitment of C1-INH to cells, StcE may also reduce the opsonization of E. coli O157:H7 by C3b and the production of the chemoattractant anaphylatoxins C3a and C5a. Because StcE is a secreted protein, the protection against complement activation may extend to the colonic epithelial cells colonized by E. coli O157:H7.
Although the Examples indicate that StcE has no effect on C1-INH-mediated inhibition of kallikrein in the absence of cells, it is reasonably expected that StcE-treated C1-INH may downregulate contact activation pathway initiated upon the interaction of Factor XII and prekallikrein with negatively charged surfaces.
In addition to its ability to sequester C1-INH to cell surfaces and cleave in the N-terminal region of C1-INH, we have discovered that StcE is capable of cleaving heavily glycosylated polypeptides and mucins, such as those found in saliva or sputum, including, but not limited to, MUC7 and gp-340/DMBT1.
The ability of StcE to cleave glycosylated polypeptides may facilitate colonization of the gut by E. coli O157:H7. The Examples below show that a StcE knockout mutant has impaired ability to form pedestals, but pedestal formation is restored by supplementation with exogenous StcE.
Elucidation of StcE functions suggests that StcE plays a role in the establishment and progression of EHEC infection and associated disease processes. StcE is therefore a promising potential target for chemotherapeutic or immune-based prevention or treatment of EHEC diseases. Active or passive immune prophylaxis using StcE as an antigen or anti-StcE antibodies may prevent the serious sequalae associated with infections by enterohemorrhagic E. coli. 
The development of an assay for StcE activity as described herein below will facilitate screening of potential therapeutics for the ability to inhibit cleavage of the C1-INH.
Preferably, purified polypeptides will be used in the methods of the present invention (i.e., to assay potential StcE inhibitors or to elicit an immune response in an animal). As used herein, a purified peptide is at least 95% pure, in that it contains no greater than 5% non StcE peptide sequences. More preferably, purified StcE polypeptides are at least 97% pure, or even as much as at least 99% or more pure. Purified polypeptides may be obtained by standard biochemical purification means, or by engineering recombinant proteins to include affinity tags that facilitate purification from complex biological mixtures.
In the examples below, polyclonal antibodies were raised against a His-tagged StcE protein that was first purified by immobilized metal affinity chromatography (IMAC) using nickel-agarose beads, followed by separation by SDS-PAGE, and excision of the band of the appropriate size from the gel. The antibodies were found to bind specifically to StcE polypeptide. One of skill in the art will appreciate that using standard methods, monoclonal antibodies useful in the practice of the present invention could be raised against a polypeptide comprising the amino acid sequence of amino acid residues 24-886 of SEQ ID NO:2 or against shorter consecutive peptide sequences thereof.
It is expected that antibodies directed against StcE will bind to and reduce StcE activity. For example, it is expected that including anti-StcE antibodies in assays of the ability of StcE-producing strains of E. coli to form pedestals would result in reduced pedestal formation. Similarly, anti-StcE antibodies could be expected to reverse StcE-potentiated C1-INH protection of cells from disruption by complement activation, to interfere with cleavage of StcE by C1-INH in a cell-free assay, and to interfere with the ability of StcE to cleave other heavily glycosylated polypeptides.
It is envisioned that an antibody preparation comprising at least one antibody that binds specification to a polypeptide comprising amino acid residues 24-886 of SEQ ID NO:2 could be used to passively immunize an animal at risk of infection by a bacterium expressing a StcE protein. This would be particularly useful for treating cows or humans believed to have been exposed to EHEC.
In view of the multiple functions that StcE appears to play in protecting the bacteria against or overcoming host defense mechanisms, purified polypeptides comprising a sequence of at least 17, 25, or 40 consecutive amino acids of SEQ ID NO:2 may be particularly useful as an immunogen for eliciting an immune response in cattle or humans. Suitably, the full length StcE or StcE E435D could be used as an immunogen.
The Examples below show that StcE is able to cleave mucins or other glycosylated polypeptides to reduce the viscosity of or solubilize the materials. It is specifically envisioned that the StcE could be used to cleave glycosylated polypeptides, thereby reducing the viscosity of the material comprising the polypeptides. The ability of StcE to cleave glycosylated polypeptides and reduce viscosity of sputum or pulmonary secretions would be of potential benefit to people with cystic fibrosis (CF). In a CF patient, the thickness of the mucus layer prevents clearance by normal mechanisms. It is envisaged that StcE cleavage of gp340 and MUC7 will reduce the aggregation activities of gp340 and MUC7, and aid mucus clearance in the CF patient. This may reduce the risk of infection and/or potentiate the efficacy of pharmaceuticals, e.g., antibiotics. Furthermore, potentiation of C1-INH-mediated inhibition of the inflammatory pathways by StcE may also benefit people with cystic fibrosis by reducing inflammation.
It is also envisioned that the ability of StcE to cleave glycosylated polypeptides and reduce the viscosity of saliva and other mucous materials would make it useful in a mucosal vaccine in conjunction with a target antigen of interest (i.e., an antigen against which one wishes to illicit an immune response in an animal) because it may enhance access of the target antigen to target cells.
As one of skill in the art would appreciate, a StcE protein comprising an amino acid sequence having minor substitutions, deletions, or additions from that of the SEQ ID NO:2 would be suitable in the practice of the present invention. Conservative amino acid substitutions are unlikely to perturb the protein's secondary structure and interfere with its activity. SEQ ID NO:2 includes the N-terminal signal sequence, which although expressed, is unlikely to be found on an isolated polypeptide. The expressed StcE protein likely undergoes post translational modification that results in cleavage of the N-terminal signal peptide. The N-terminus of secreted StcE was purified from supernatants of an E. coli K-12 strain carrying p0157 was sequenced. As predicted, the N-terminal residues of secreted StcE (designated StcE′) correspond to residues 24-27 of SEQ ID NO:2.
It is specifically envisioned that isolated polypeptides having less than the full length sequence of amino acid residues 24-886 of SEQ ID NO:2 will be useful in the practice of the present invention. StcE polypeptides that are truncated at the N-terminal or C-terminal regions or having minor sequence variations may retain the ability to bind to and/or cleave C1-INH, to promote pedestal formation, to potentiate C1-INH mediated protection of cells, or cleave other glycosylated polypeptides other than or in addition to C1-INH. Whether a protein retains binding or proteolytic activity of can be evaluated using the methods set forth herein in the Examples, or by any suitable method.
It is expected that purified StcE polypeptides that are truncated at the N-terminal or C-terminal regions or a polypeptide comprising an amino acid sequence comprising at least 17 consecutive amino acid residues of SEQ ID NO:2 may be used as an antigen against which antibodies specific for StcE may be raised. Preferably, the polypeptide comprises at least 25 consecutive amino acid residues of SEQ ID NO:2. More preferably still, the polypeptide comprises at least 40 consecutive amino acid residues of SEQ ID NO:2. One of ordinary skill in the art could easily obtain any of the various polypeptides comprising a portion of SEQ ID NO:2 by subcloning a sequence encoding the polypeptide into an expression vector, introducing the expression vector into a suitable host cell, culturing the cell, and isolating the expressed polypeptide using standard molecular biological techniques.
Using the teachings of the specification, one of skill in the art could readily obtain a polypeptides having at least 95% amino acid identity to amino acid residues 24-886 of SEQ ID NO:2. Suitably, the polypeptide comprises a sequence having at least 97% or at least 99% amino acid identity to amino acid residues 24-886 of SEQ ID NO:2.
The following non-limiting examples are intended to be purely illustrative.
EXAMPLES
I. Identification and Characterization of StcE
A list of bacterial strains and plasmids is found in Table 1. Strains were constructed and plasmids were maintained in either E. coli K-12 DH1 or C600 unless otherwise noted. Recombinant DNA manipulations were performed by standard methods.
Enterohemorrhagic Escherichia coli strains EDL933 and EDL933cu (lacking plasmid pO157) and WAM2371 (enteropathogenic E. coli strain E2348/69) were provided by Dr. Alison O'Brien of the Uniformed Services University. WAM2035 (C600/pO157) was provided by Dr. Hank Lockman of the Uniformed Services University. WAM2516 (Citrobacter rodentium strain DBS100) was provided by Dr. David Schauer of the Massachusetts Institute of Technology. The Diarrheagenic E. coli (DEC) collection was a gift from Dr. Tom Whittam of the University of Pennsylvania. WAM2547 was created by transforming pLOF/Km (a gift from Dr. Victor De Lorenzo of the GBF-National Research Centre for Biotechnology, Germany) into the donor strain S17(λpir).







TABLE 1







Bacterial strains and plasmids used in this study.









Strain
Relevant phenotype or plasmid genotype
Source





C600
E. coli K-12
this laboratory


DH1
laboratory strain of E. coli
this laboratory


S17( pir)
E. coli donor strain for conjugation
this laboratory


BL21(DE3)
E. coli strain for protein overexpression
Novagen


EDL933
wild-type EHEC strain
A. O'Brien


EDL933cu
EHEC strain EDL933 cured of pO157
A. O'Brien


WAM2371
EPEC strain E2348/69
A. O'Brien


WAM2516
C. rodentium strain DBS100
D. Schauer


DEC strains
Diarrheagenic E. coli collection
T. Whittam


WAM2035
C600/pO157::Tn801 (ampr)
H. Lockman


WAM2515
C600/pO157::Tn801 (ampr nalr)
this study


WAM2297
DH1/pBluescript II SK+ (ampr)
this laboratory


WAM2547
S17( pir)/pLOF/Km (ampr kanr)
this study


WAM2553
C600/pWL104 (ampr kanr)
this study


WAM2562
DH1/pWL105 (ampr)
this study


WAM2572
BL21(DE3)/pWL107 (kanr)
this study


WAM2726
BL21(DE3)/pTEG1 (kanr)
this study


WAM2815
EDL933 with stcE replaced by cat
this study


WAM2997
WAM2815 strain with stcE at the Tn7att site
this study


pLOF/Km
pGP704 carrying miniTn10kan
V. De Lorenzo


pO157
92 kb plasmid of EDL933; Tn801 at base 5413
H. Lockman


pBluescript II SK+
cloning vector
Stratagene


pET24d(+)
6xHis overexpression vector
Novagen


pWL104
pO157::miniTn10kan inserted at base 23772
this study


pWL105
pBluescript II SK+/bases 1-2798 of L7031
this study


pWL107
pET24d(+)/bases 138-2795 of L7031
this study


pTEG1
pWL107 with amino acid change E435D
this study









WAM2515 is a spontaneous nalidixic acid-resistant mutant of WAM2035. WAM2553 was created as described below, containing a mini-Tn10kan insertion at base 23772 of pO157 (accession #AF074613). This plasmid is designated pWL104. WAM2297 is pBluescript II SK+ in DH1. pWL105 was constructed by amplifying bases 1 to 2798 of the promoter and gene L7031/stcE from pO157 by polymerase chain reaction (PCR) using primer pairs 5′-CCCTCGAGTTTACGAAACAGGTGTAAAT-3′ (SEQ ID NO:4) and 5′-CCTCTAGATTATTTATATACAACCCTCATT-3′ (SEQ ID NO: 5); and cloning the product into the XbaI-XhoI sites of pBluescript II SK+ (Stratagene); WAM2562 is DH1 containing pWL105. pWL107 was constructed by PCR amplification of bases 138 to 2798 of the promoter and gene L7031/stcE from pO157 by PCR using primer pairs 5′-CCGAGCTCCGATGAAATTAAAGTAT-CTGTC-3′ (SEQ ID NO:6) and 5′-CCTCGAGTTTATATACAACCCTCATTG-3′ (SEQ ID NO:7); and cloning the PCR product into the SacI-XhoI sites of pET-24d(+) (Novagen); WAM2572 is BL21(DE3) (Novagen) transformed with pWL107. The creation of WAM2726 is described below. All chemicals were purchased from Sigma (St. Louis, Mo.) unless stated otherwise.
Cell Lines
All cell lines were maintained in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (HyClone) and 10 μg/ml gentamicin at 37° C. with 5% CO2. The human T cell line Jurkat clone E6-1, the human promyelocytic leukemia line HL-60, and the human B cell lymphoma line Raji were obtained from ATCC, the human promyelocytic leukemia line U937 was a gift from Dr. Jon Woods of the University of Wisconsin-Madison, and the human T cell lymphoma line MOLT-4 was a gift from Dr. David Pauza of the University of Wisconsin-Madison.
Aggregation Assays
Bacterial strains were grown overnight in Lennox L broth (with antibiotic selection when appropriate) at 37° C. with agitation. Cultures were washed once with phosphate buffered saline (PBS) and resuspended in 1/10 the original culture volume in PBS. Cultures were lysed in a French Press at 20,000 lbs/in2. The resulting lysates were spun at 1000×g to remove debris and protein concentrations were determined by the Bradford protein assay (Bio-Rad). Tissue culture cells were suspended at 106 cells/ml in RPMI 1640 and 50 μg/ml gentamicin with 10% FBS or human serum. Fifty μg/ml of lysates or 50-200 ng/ml purified StcE-His (see below) were added to cells and incubated for two hours at 37° C. in 5% CO2. Cells were agitated for one minute to disrupt spontaneous aggregates before visualization. Similar assays were performed in the absence of serum or with ammonium sulfate-precipitated fractions of human serum (see below); cells were washed once in RPMI 1640 and resuspended at 1×106 cells/ml in RPMI 1640 with 50 μg/ml gentamicin (and human serum fractions, if indicated) before the addition of lysates or StcE-His. When indicated, ethylenediaminetetraacetic acid (EDTA) or bathophenanthroline-disulfonic acid (BPS) were added to the assays at a final concentration of 5 mM.
Identification of stcE
WAM2515 was mated with WAM2547 as described (7). Transconjugants were plated onto LB plates containing 100 μg/ml ampicillin, 50 μg/ml kanamycin, and 50 μg/ml nalidixic acid. Transconjugants were resuspended in 1×TES, washed once with 1×TES, and pO157/pO157::mini-Tn10kan were isolated by midi-prep (Qiagen). pO157/pO157::mini-Tn10kan were transformed into C600 and plated onto LB plates containing 100 μg/ml ampicillin and 50 μg/ml kanamycin. Transformants were grown overnight in Lennox L broth containing 100 μg/ml ampicillin and 50 μg/ml kanamycin at 37° C. with agitation and lysates were screened for the ability to aggregate Jurkat cells as described above. pO157::mini-Tn10kan was isolated from clones lacking the ability to aggregate Jurkat cells and the location of the transposable element was identified by sequence analysis. One clone unable to aggregate Jurkat cells was designated WAM2553.
Purification of Recombinant StcE-His
StcE-His was purified according to the manufacturer's instructions (Novagen). Briefly, WAM2572 was induced to produce StcE-His by the addition of IPTG to 1 mM at an O.D. of 0.5 followed by vigorous aeration at 37° C. for approximately three hours. The cells were lysed in a French Press at 20,000 lbs/in2 and the resulting lysate was centrifuged at 20,000×g for 15 minutes. The insoluble pellet was resuspended in a buffer containing 5 mM imidazole and 6 M urea and the inclusion bodies were solubilized for one hour on ice. This fraction was incubated with nickel-agarose beads (Qiagen) overnight at 4° C., and the beads were washed three times with a buffer containing 60 mM imidazole and 6 M urea. Purified StcE-His was eluted from the beads with a buffer containing 300 mM imidazole and 6 M urea. Eluted StcE-His was dialyzed against three changes of PBS/20% glycerol at 4° C. to remove the imidazole and urea. Protein concentration was determined by SDS-PAGE using purified β-galactosidase as a standard. At our request, polyclonal antibodies to purified StcE-His were prepared in rabbits by Cocalico Biologicals, Inc. Briefly, purified StcE-His was electrophoresed on an 8% polyacrylamide gel and stained with Coomassie Brilliant Blue. StcE-His was excised from the gel and injected into rabbits. Rabbits were boosted with StcE-His once a month for six months prior to exsanguinations.
Two-dimensional Gel Electrophoresis
Human serum was fractionated by ammonium sulfate precipitation, dialyzed against three changes of RPMI 1640 (Gibco) overnight at 4° C., and protein concentration was determined by Bradford assay (Bio-Rad). When indicated, protein A-sepharose was used to remove fractions of IgG. Two-dimensional electrophoresis was performed according to the method of O'Farrell (8) by Kendrick Labs, Inc. (Madison, Wis.) as follows: isoelectric focusing was carried out on 25 μg of 30-60% ammonium sulfate-fractionated human serum removed of IgG in glass tubes of inner diameter 2.0 mm using 2.0% pH 3.5-10 ampholines (Amersham Pharmacia Biotech) for 9600 volt-hrs. Fifty ng of an IEF internal standard, tropomyosin, was added to each sample. This protein migrates as a doublet with lower polypeptide spot of MW 33,000 and pI 5.2; an arrow on the stained gel marks its position. The enclosed tube gel pH gradient plot for this set of ampholines was determined with a surface pH electrode.
After equilibration for 10 min in buffer “0” (10% glycerol, 50 mM dithiothreitol, 2.3% SDS and 0.0625 M Tris, pH 6.8) each tube gel was sealed to the top of a stacking gel that is loaded on the top of a 8% acrylamide slab gel (0.75 mm thick). SDS slab gel electrophoresis was carried out for about 4 hrs at 12.5 mA/gel. The following proteins (Sigma) were added as molecular weight standards to a well in the agarose which sealed the tube gel to the slab gel: myosin (220 kDa), phosphorylase A (94 kDa), catalase (60 kDa), actin (43 kDa), carbonic anhydrase (29 kDa), and lysozyme (14 kDa). These standards appear as bands on the basic edge of the special silver-stained (O'Connell and Stults 1997) 8% acrylamide slab gel. The gel was dried between sheets of cellophane with the acidic edge to the left.
A similar gel was run as described above with the following differences: 250 μg of 30-60% ammonium sulfate-fractionated human serum was loaded onto the IEF gel; the second dimension was run on a 10% acrylamide slab gel and stained with Coomassie Brilliant Blue.
Far Western Blot Analysis
One hundred μg of 30-60% ammonium sulfate-fractionated human serum was run on a two-dimensional gel as described above but without staining. After slab gel electrophoresis the gel for blotting was transferred to transfer buffer (12.5 mM Tris, pH 8.8, 86 mM glycine, 10% methanol) and transblotted to PVDF membrane overnight at 200 mA and approximately 50 volts/gel. The PVDF membrane was blocked with 2% milk (Difco) in buffer AD (20 mM Tris, pH 7.5, 100 mM NaCl, 10% glycerol, 0.01% Tween-20) at 4° C. Two μg/ml purified StcE-His was added to the PVDF membrane and allowed to incubate two hours at 4° C. The membrane was washed with buffer AD and blocked with 2% milk in buffer AD. The membrane was reacted with polyclonal anti-His antibody conjugated with horse-radish peroxidase (Santa Cruz), washed with buffer AD, and developed with the LumiGlo chemiluminescence detection system (Kirkegaard & Perry Laboratories). The PVDF membrane was then stripped (62 mM Tris, pH 6.8, 2% SDS, 10 mM β-mercaptoethanol (β-ME), 30 min, 50° C.), washed with buffer AD, reacted as above with only the His-HRP antibody, and developed.
Mass Spectrometry
Of the three spots in human serum that reacted with purified StcE-His as identified by Far Western blotting, only the leftmost spot (the most acidic) of approximately 100 kDa was accessible for analysis by mass spectrometry. This spot was cut from the Coomassie Brilliant Blue-stained 10% slab gel and sent to the Protein Chemistry Core Facility at the Howard Hughes Medical Institute/Columbia University for analysis. The spot was digested with endoproteinase Lys-C and analyzed by MALDI-MS. The peptide pattern was compared against known human proteins in the SWISS-PROT database and was identified as plasma protease C1 inhibitor.
Electrophoresis and Immunoblot Analyses
Fifty μg whole and ammonium sulfate-precipitated human serum fractions were incubated with 500 ng purified StcE-His in 500 μl buffer AD for two hours at room temperature and precipitated with 10% trichloroacetic acid (TCA) on ice for one hour. Precipitates were collected by centrifugation, resuspended in 1× sample buffer (2% SDS, 10% glycerol, 5% P-ME, 1 mM bromophenol blue, 62 mM Tris, pH 6.8), and heated to 95-100° C. for 5 min prior to electrophoresis on 8% polyacrylamide gels. Separated proteins were transferred to Hybond ECL nitrocellulose (Amersham Pharmacia Biotech) as described (9) for immunoblot analysis. Blots were blocked with 5% milk in TBST (154 mM NaCl, 20 mM Tris, pH 7.6, 0.1% Tween-20), probed with a polyclonal anti-C1 inhibitor antibody (Serotec) and then with HRP-conjugated anti-rabbit secondary antibody (Bio-Rad) before developing as described above.
Sixteen μg purified C1 inhibitor (Cortex Biochem) were incubated with 4.8 μg purified StcE-His in 480 μl buffer AD at room temperature; 30 μl of the reaction were removed at various time points, suspended in 1× sample buffer, and heated to 95-100° C. for 5 min prior to electrophoresis on 8% polyacrylamide gels. Separated proteins were transferred to nitrocellulose and reacted with anti-C1 inhibitor antibody as described above.
EDL933, EDL933cu, WAM2035, and WAM2553 were grown in Lennox L broth at 37° C. overnight, centrifuged, and the culture supernatant was removed. The supernatant was precipitated with ammonium sulfate and the 0-60% fraction was resuspended at 1/100 the original culture volume and dialyzed against three changes of PBS overnight at 4° C. Twenty PI of the dialyzed supernatants and 30 μg of EDL933, EDL933cu, WAM2035, and WAM2553 lysates were suspended in 1× sample buffer and heated to 95-100° C. for 5 min prior to electrophoresis on 8% polyacrylamide gels. Separated proteins were transferred to Hybond ECL nitrocellulose and reacted with polyclonal anti-StcE-His antibody, followed by anti-rabbit-HRP secondary antibody.
Casein Proteolysis Assay
Various concentrations of StcE-His were incubated with BODIPY FL-conjugated casein for various times using the EnzChek Protease Assay Kit (Molecular Probes, Inc.) and the increase in fluorescence was measured with a fluorimeter as per the manufacturer's instructions.
Lysates of E. coli Strains Carrying pO157 Induce the Aggregation of Transformed Human T Cell Lines in a Serum-dependent Manner.
To determine the consequence of pO157-containing E. coli products on Jurkat cells, a human T cell lymphoma line, 50 g/ml of lysates of strains EDL933, EDL933cu, WAM2035, WAM2371, WAM2516, and C600 were applied to 1×106 Jurkat cells/ml in RPMI 1640 with 10% FBS and 50 μg/ml gentamicin for two hours at 37° C. in 5% CO2. After agitation for one minute to disrupt spontaneous aggregates, Jurkats were observed for the induction of aggregation. Lysates of E. coli strains carrying pO157 induced the aggregation of Jurkat cells while lysates of strains lacking pO157 did not (FIG. 1). Lysates of other pathogenic bacteria such as enteropathogenic E. coli strain E2348/69 (WAM2371) and C. rodentium (WAM2516) capable of inducing the attaching and effacing (A/E) phenotype on intestinal epithelial cells and carrying large virulence plasmids different from pO157 were unable to induce the aggregation of Jurkat cells. To determine whether this effect was specific for Jurkat cells or could induce the aggregation of a broader host cell range, 1×106 cells/ml in RPMI 1640 with 10% FBS and 50 μg/ml gentamicin of another human T cell lymphoma line, MOLT-4, two human promyelocytic leukemia cell lines, HL-60 and U937, and a human B cell lymphoma line, Raji, were treated with 50 μg/ml of EDL933 and WAM2035 lysates for two hours at 37° C. in 5% CO2. pO157-containing lysates aggregated MOLT-4 cells but not HL-60, U937, or Raji cells (data not shown), indicating T cell specificity for the phenotype.
To determine the serum requirement for the induction of aggregation, 50 μg/ml of lysates of EDL933 and WAM2035 were applied to 1×106 Jurkat cells/ml with 10% human serum and 50 μg/ml gentamicin for two hours at 37° C. in 5% CO2. As seen with FBS, pO157-containing lysates were able to induce the aggregation of Jurkat cells in the presence of human serum. However, EDL933 and WAM2035 lysates were unable to induce the aggregation of Jurkat cells under the same conditions in the absence of serum. To further characterize the component(s) of human serum responsible for mediating Jurkat cell aggregation in the presence of StcE, we fractionated human serum by ammonium sulfate precipitation followed by dialysis in RPMI 1640. We found that 0-30% and 30-60%, but not 60-100%, ammonium sulfate-precipitated human serum was able to mediate aggregation of Jurkat cells in the presence of StcE. This indicates a factor or factors in serum is required for the aggregation of Jurkat cells when treated with lysates of pO157-containing bacteria.
Identification and Cloning of stcE
To localize the gene(s) on pO157 responsible for the induction of aggregation of human T cell lines, we subjected pO157 to mutagenesis using a minitransposon. Lysates of recombinant strains of E. coli containing pO157 mutagenized with mini-Tn10kan were tested for the ability to aggregate Jurkat cells in RPMI 1640 with 10% FBS and 50 g/ml gentamicin. pO157::mini-Tn10kan was isolated from clones whose lysates were unable to induce the aggregation of Jurkat cells. The location of the transposon insertion in WAM2553 was determined by sequence analysis and mapped to position 23772 of pO157. The open reading frame in which the transposon inserted was designated L7031 (10) and is located immediately 5′ to the general secretory apparatus on pO157. L7031/stcE was amplified and cloned into the XbaI-XhoI sites of pBluescript II SK+. Lysates of WAM2562 induced aggregation of Jurkat cells in the presence of serum, whereas lysates of WAM2297 (DH1 carrying pBluescript II SK+) did not, which confirms that the stcE gene is responsible for the phenotype.
Based on sequence analysis, we concluded that the translational start site for StcE was more likely to begin at base 138 than at base 102 (10). We therefore amplified the coding sequence for stcE from bases 138 to 2798 by PCR and cloned the gene in frame with a 6×His-tag at the 3′ end of the fusion in pET24d(+). We were able to overexpress and purify a recombinant his-tagged form of StcE (StcE-His) (FIG. 2); this purified fusion protein was able to aggregate Jurkat cells in the presence of serum at a variety of concentrations (data not shown).
Localization and Characterization of StcE
Using antiserum to StcE-His, we performed immunoblot analysis to examine the expression and secretion of StcE by E. coli. StcE is expressed by E. coli strains carrying pO157 at 37° C. in Lennox L broth but not in strains lacking pO157 or harboring a transposon insertion in stcE (FIG. 3). Additionally, StcE is released into the culture supernatant by strains carrying pO157 under the same growth conditions (FIG. 3). As StcE contains a putative cleavable N-terminal signal sequence, it is possible that StcE is actively released from the bacterium by the general secretory apparatus encoded on pO157.
StcE-mediated Jurkat cell aggregation is inhibited by the addition of ion chelators such as EDTA, a broad chelator of divalent cations, and BPS, a chelator specific for zinc and iron ions (data not shown). This suggests that StcE has a requirement for one or more divalent cations, most likely zinc. This is supported by the presence of an exact match to the histidine-rich consensus active site for metalloproteases, which coordinate zinc ions for activity (see discussion).
StcE-His interacts with a human serum protein(s) of approximately 105 kDa. To identify the factor(s) in human serum responsible for mediating Jurkat cell aggregation in the presence of StcE, the 30-60% ammonium sulfate-precipitated fraction of human serum was separated on a two-dimensional gel and transferred to a PVDF membrane. Using purified StcE-H is as a probe, we performed Far Western blot analysis on the PVDF membrane, detecting any interactions between StcE-His and human serum proteins with an HRP-conjugated anti-His antibody. We found that StcE-His interacts with three spots of approximately 105 kDa ranging from very acidic to very basic in isoelectric point (data not shown). Probing the same membrane with only the HRP-conjugated anti-His antibody revealed that the three spots of approximately 105 kDa were specific for StcE-His (data not shown).
To identify these proteins, the 30-60% ammonium sulfate-precipitated fraction of human serum was removed of IgG and separated on another two-dimensional gel and either special silver stained or stained by Coomassie Brilliant Blue. The most acidic of the three spots (the leftmost spot) was well isolated from other proteins and excised from the Coomassie Brilliant Blue-stained gel. This spot was digested by endoproteinase Lys-C and analyzed by MALDI-MS. A comparison of the resulting peptide pattern with known human proteins in the SWISS-PROT database revealed a match with human plasma protease C1 inhibitor.
Cleavage of C1 Inhibitor by StcE-His
To confirm the interaction between StcE and human C1 inhibitor and to test the possibility that StcE may proteolyze C1 inhibitor, whole and ammonium sulfate-precipitated fractions of human serum were mixed with StcE-His, separated by SDS-PAGE, and transferred to nitrocellulose for immunoblot analysis. Using an anti-human C1 inhibitor antibody, we detected the presence of C1 inhibitor in samples lacking StcE-His and the absence of C1 inhibitor in samples containing StcE-His (FIG. 4). As predicted by Jurkat cell aggregation, the 0-30% and 30-60% ammonium sulfate-precipitated fractions of human serum were enriched for C1 inhibitor compared to the 60-100% fraction. After treatment with StcE-His, however, little to no C1 inhibitor could be detected in any of the fractions. The addition of EDTA or BPS to the mixture prevented the disappearance of C1 inhibitor from the serum samples, indicating a specific requirement for divalent cations, most likely zinc, for StcE activity (data not shown).
To confirm that the proteolysis of C1 inhibitor was a direct result of an interaction with StcE-His, we mixed purified human C1 inhibitor with StcE-His and removed aliquots of the reaction at various time points for analysis by immunoblot. Using an anti-human C1 inhibitor antibody, we detected the disappearance of a 105 kDa band corresponding to full-length C1 inhibitor and the appearance of an approximately 60 kDa cleavage product in a time-dependent manner (FIG. 5).
Examination of Patient Fecal Filtrates for StcE
Freshly passed stool samples from children with culture-positive E. coli O157:H7 (n=6), Campylobacter jejuni (n=2), Shigella B (n=2), or Clostridium difficile (n=2) infections were diluted 1:10 in PBS and passed through a 0.45 μm filter. Thirty μl of thawed filtrate was suspended in 1× sample buffer, heated (95-100° C. for 5 min) and electrophoresed on 8% polyacrylamide gels. Separated proteins were transferred to nitrocellulose and probed with a polyclonal antibody to StcE-His as described above. Twenty μl of the same samples was added to 1×106/ml Jurkat cells in 10% FCS and gentamicin (50 μg/ml) for 24 hours at 37° C. in 5% CO2 to determine the ability of the filtrates to aggregate Jurkat cells.
Construction and Analyses of StcE E435D-His Mutant
The StcE E435D-His mutant was created using the PCR-based method of overlap extension (Horton et al. 1993). The first two PCR reactions were (i) stcE top strand primer 587 (5′-CCGCTCCGGTGAACTGGAGAATA-3′) (SEQ ID NO:8) with its partner mutagenic primer 592 (5′-GACCATAATTATGACCAACATCATGACTGA-3′) (SEQ ID NO:9) and (ii) stcE bottom strand primer 573 (5′-CCTTATCTGCGGAGGCTGTAGGG-3′) (SEQ ID NO:10) with its partner mutagenic primer 574 (5′-TGAGTTCAGTCATGATGTTGGTCATAATTAT-3′) (SEQ ID NO:11). Each reaction used 50 μM of each primer, about 100 ng of template DNA, and Deep Vent polymerase (New England Biolabs) in a 100 μl reaction. The reactions were run in a thermocycler under appropriate conditions (11) and the resulting products were purified on a 1% agarose gel using the QIA-quick Gel Extraction Kit (Qiagen). The next PCR reaction contained 5 μl each of the gel-purified fragments, along with the stcE primers 587 and 573 and Deep Vent polymerase in a 100 μl reaction. The PCR products were gel-purified as above and cut with the restriction endonucleases PmeI and BsrG1. pWL107 was also cut with PmeI and BsrG1 and the mutant PCR product was ligated into pWL107, creating pTEG1. The base substitution was confirmed by sequence analysis. pTEG1 was transformed into E. coli strain BL21(DE3) to create WAM2726 and StcE E435D-His (SEQ ID NO:19) was overexpressed and purified from this strain as described above. The purified protein was then analyzed for its ability to aggregate Jurkat cells as described above.
Purified C1-INH (one μg) was mixed with or without StcE-His (one μg) or StcE E435-His (one μg) overnight at room temperature in 500 μl buffer AD, precipitated with TCA (to 10%), electrophoresed on an 8% polyacrylamide gel, and transferred to nitrocellulose before analysis by immunoblot with an anti-C1-INH antibody as described above.
Purified C1-INH (500 ng) and human serum (50 μg) were electrophoresed on an 8% polyacrylamide gel in duplicate and the separated proteins were transferred to nitrocellulose for Far Western analysis. Essentially the same protocol was followed as described above with the following difference: one blot was probed with purified StcE-His (2 μg/ml) and the other with purified StcE E435D-His (2 μg/ml).
Colony Blot Analysis
A one kb fragment of stcE was PCR amplified from pO157 using the primers stcE5′846 (5′-GAGAATAATCGAATCACTTATGCTC-3′) (SEQ ID NO:12) and stcE3′1773 (5′-CGGTGGAGGAACGGCTATCGA-3′) (SEQ ID NO:13) under standard reaction conditions. The PCR product was purified on a 1% agarose gel using the QIA-quick Gel Extraction Kit (Qiagen) and fluorescein-labeled using the ECL random prime labeling system (Amersham Life Science). Bacterial strains from the DEC collection, EDL933, and EDL933cu were patched onto sterile Magna Lift nylon transfer membranes (Osmonics) on LB plates and grown overnight at room temperature. Colonies were lysed by placing the membranes on 3MM Whatman paper soaked in 0.5 M NaOH. Neutralization was performed by placing the membranes first on 3 mM Whatman paper soaked in 1 M Tris, pH 7.5 and then on 3MM Whatman paper soaked in 0.5 M Tris, pH 7.5/1.25 M NaCl. DNA was then crosslinked using a UV stratalinker. The blots were pre-hybridized in Church buffer (0.5 M dibasic sodium phosphate, pH 7.3, 7% SDS, 1% BSA, 1 mM EDTA) at 65° C. for one hour before the addition of the labeled probe. Hybridization proceeded overnight at 65° C. The membranes were then washed at 65° C. in 1×SSC/0.1% SDS for 15 minutes and then in 0.5×SSC/0.1% SDS for 15 minutes. The membranes were incubated with an anti-fluorescein labeled, HRP-conjugated antibody. The membrane was developed using the LumiGLO Chemiluminescent Substrate Kit (Kirkegaard and Perry Laboratories).
PCR Analysis of stcE
Oligonucleotides were designed to amplify by PCR regions of stcE to cover the length of the  ̃2.8 kbp promoter and gene. Primers stcE5′1 (5′-TTTACGAAACA-GGTGTAAATATGTTATAAA-3′) (SEQ ID NO:14) and stcE3′845 (5′-CAGTTCACCG-GAGCGGAACCA-3′) (SEQ ID NO:15) covered the first third, stcE5′846 and stcE3′1773 covered the middle third, and stcE5′1774 (5′-GCTTCAGC-AAGTGGAATGCAGATAC-3′) (SEQ ID NO:16) and stcE3′2798 (5′TTATTTAT-ATACAACCCTCATTGACCTAGG-3′) (SEQ ID NO:17) covered the final third. Genomic DNA was isolated from E. coli strains DEC3A-E, DEC4A-E, DEC5A-E, EDL933, and EDL933cu using the Wizard Genomic DNA Purification Kit (Promega) as per the manufacturer's instructions. PCR was performed using 20 pM of each primer, about 100 ng of template DNA, and Deep Vent polymerase (New England Biolabs) in a 100 μl reaction. The reactions were run in a thermocycler under standard conditions. PCR products were electrophoresed on 1% agarose gels and visualized with ethidium bromide.
Isolation and Analyses of Bacterial Culture Supernatants
E. coli strains DEC3A-E, DEC4A-E, DEC5A-E, EDL933, and EDL933cu were grown in Lennox L broth at 37° C. overnight. Culture supernatants were harvested by centrifugation at 4° C. for 15 minutes at 10,000×g and filtered through a 0.45 μm filter. Supernatants were precipitated with ammonium sulfate to 75% saturation. The precipitates were centrifuged for 15 minutes at 16,000×g at 4° C. and resuspended in buffer AD. The resuspended precipitates were dialyzed against three changes of AD buffer overnight to remove residual ammonium sulfate.
Purified CI-INH (one μg) was mixed with 200 μl of ammonium sulfate-precipitated culture supernatants at room temperature overnight in a total volume of 500 μl buffer AD before precipitation with TCA (to 10%) and electrophoresis on 8% polyacrylamide gels. Separated proteins were transferred to nitrocellulose and immunoblot analysis was performed with an anti-C1-INH antibody as described above. Culture supernatants alone were separated on 8% polyacrylamide gels and transferred to nitrocellulose before immunoblot analysis was performed using an anti-StcE-His antibody as described above.
Specificity of StcE-His for C1-INH
To evaluate the specificity of StcE-His, potential target proteins (listed in Table 2), target protein (2 μg) was mixed with either StcE-His (1.28 μg) or Pseudomonas aeruginosa elastase (20 ng) (Calbiochem EC# 3.4.24.26) overnight at 37° C. in 500 μl buffer AD (20 mM Tris, pH 7.5, 100 mM NaCl, 10% glycerol, 0.01% Tween-20) and precipitated with TCA (to 10%) prior to electrophoresis on 8-10% polyacrylamide gels. Proteins in the gels were then Coomassie-stained or transferred to nitrocellulose for immunoblot analysis as above.
StcE is able to cleave both purified and serum-associated C1-INH. Only C1-INH was cleaved by StcE-His; the sizes and staining intensities of all other potential substrates were the same in the presence and absence of StcE-His. In contrast, elastase degraded most of the proteins tested. Elastase treatment of C1-INH normally produces an inactive 95 kDa product (12), whereas treatment of C1-INH with StcE-His results in  ̃60-65 kDa C1-INH fragment(s) (FIG. 6). This indicates that the StcE cleavage site of C1-INH is distinct from that used by elastase. We employed a sensitive fluorimetric assay based on the digestion of a BODIPY FL-labeled casein substrate (EnzChek, Molecular Probes) to analyze further the ability of StcE-His to act as a non-specific endoprotease. Serial two-fold dilutions of StcE-His or P. aeruginosa elastase were mixed with the casein substrate per the manufacturer's instructions before fluorescent measurement of casein degradation. StcE-His was unable to degrade casein even at high protein concentrations (up to 6.4 μg/unit of volume), while elastase was able to act on casein at lower concentrations (range: 0.5 ng to 1 μg/unit of volume) (data not shown).







TABLE 2







Proteolysis of substrates incubated with


StcE-His or P. aeruginosa elastase









Substrate
StcE
Elastase





C1 inhibitor (Cortex Biochem, San Leandro, CA)
+
+


α2-antiplasmin (Calbiochem, San Diego, CA)
−
+


α1-antitrypsin (Sigma, St. Louis, MO)
−
+


α1-antichymotrypsin (Sigma, St. Louis, MO)
−
+


antithrombin (Enzyme Research Labs, South Bend, IN)
−
+


α2-macroglobulin (Calbiochem, San Diego, CA)
−
+


von Willebrand factor (gift from Dr. D. Mosher,
−
N.D.


UW-Madison)


collagen IV (Rockland, Gilbertsville, PA)
−
−


fibronectin (Calbiochem, San Diego, CA)
−
+


serum albumin (New England Biolabs, Beverly, MA)
−
N.D.


IgA1 (Cortex Biochem, San Leandro, CA)
−
+


Elastin (Sigma, St. Louis, MO)
−
+


Gelatin (BioRad, Hercules, CA)
−
+





N.D. = not done


Two μg of the indicated protein substrates were mixed with 1.28 μg StcE-His or 20 ng P. aeruginosa elastase overnight at 37° C. prior to electrophoresis by SDS-PAGE and staining with Coomassie Brilliant Blue. StcE was unable to digest any of the proteins tested other than C1-INH, while P. aeruginosa elastase had activity against a broad range of targets.






Detection of StcE in Feces.

The Shiga-like toxin has been identified in the feces of patients infected with E. coli O157:H7 (13, 14). To demonstrate that StcE is produced in vivo during an E. coli O157:H7 infection, we examined fecal filtrates collected from patients with E. coli O157:H7 and non-E. coli O157:H7-mediated diarrhea for the presence of StcE antigen and activity. Twelve fecal samples were diluted in PBS and filtered before analysis by immunoblot with polyclonal antibodies to StcE-His. A strongly reactive band with a molecular weight similar to StcE was present in the filtrate from one child infected with E. coli O157:H7 (FIG. 7, sample 2). Because StcE is able to mediate the aggregation of T cells, we examined the ability of the twelve fecal filtrates to aggregate Jurkat cells. Twenty μl of each filtrate was added to 5×105 Jurkat cells in the presence of 10% FCS. The one sample that contained a StcE-reactive species aggregated Jurkat cells to the same extent as 50 ng/ml purified StcE-His; all other samples were negative in this assay, even after 24 hours of incubation (data not shown).
StcE Contains a Zinc Metalloprotease Active Site.
As the predicted StcE amino acid sequence has a consensus Zn2+-ligand binding site of metalloproteases (434: HEVGHNYGLGH) (SEQ ID NO:3), we examined the possibility that the glutamic acid residue at position 435 is critical for the proteolysis of C1-INH. This amino acid in other zinc metalloproteases acts as the catalytic residue for proteolysis (15) (16), and other researchers have shown that a conservative amino acid substitution from glutamic to aspartic acid disrupts the activity of the protease while maintaining its structure (15). By introducing a single change in the sequence of stcE at base 1442 from an A to a T, we created the same mutation and examined the ability of the recombinant mutant (StcE E435D-His) to digest C1-INH. While StcE-His is able to degrade C1-INH, we observed no such cleavage with the mutant protein (FIG. 8A) under the same conditions. The results of Far Western analysis (FIG. 8B) suggested that StcE E435D-His is unable to bind to C1-INH (or a similarly sized protein in human serum), which seemed to suggest that glutamic acid 435 is necessary for both binding and cleavage of C1-INH. The StcE-mediated aggregation of Jurkat cells was also affected by the E435D mutation. Jurkat cells will aggregate in response to StcE-His at concentrations as low as 1 ng/ml, while cells treated with as much as 200 ng/ml StcE E435D-His did not aggregate (data not shown). Thus, the glutamic acid residue at position 435 is critical for StcE-mediated aggregation of Jurkat cells, as well as proteolysis of C1-INH. However, based on subsequent results of studies involving StcE potentiation of C1-INH-mediated protection of cells, described in detail below, it appears that StcE E435D-His can in fact interact with C1-INH.
Detection of stcE among Diarrheagenic E. coli Strains.
In order to establish the prevalence of stcE among other pathogenic strains of E. coli, we examined the Diarrheagenic E. coli (DEC) collection, a reference set of 78 E. coli strains provided by Dr. Tom Whittam of the University of Pennsylvania, for the presence of stcE. This collection contains a variety of enterohemorrhagic, enteropathogenic, and enterotoxigenic E. coli strains of different serotypes isolated from humans, non-human primates, and other mammals that are associated with disease symptoms, including diarrhea, hemorrhagic colitis, or HUS. The DEC collection is divided into 15 subgroups based on electrophoretic type, which is indicative of the genetic similarity of one strain to another. By using colony blot analysis, we found that all O157:H7 strains of E. coli (DEC3 and DEC4) contain DNA that hybridizes with an internal one kb region of stcE (Table 3). Surprisingly, three of five enteropathogenic 055:H7 strains of E. coli (DEC5A, C, & E) also hybridized with the stcE probe. Because O157:H7 strains are thought to have evolved from an O55:H7 predecessor, this result suggests a source of the stcE gene for current O157:H7 strains of E. coli. None of the other strains in the DEC collection hybridized with the stcE probe by colony blot analysis.
To confirm the presence of the gene among the stcE-positive groups in the DEC collection, we isolated genomic DNA from DEC3A-E, DEC4A-E, DEC5A-E, EDL933, and EDL933cu and used oligonucleotide pairs designed to amplify regions of stcE by PCR. Three primer sets were chosen to amplify stcE and its promoter from bases 1-845, 846-1773, and 1774-2798. An appropriately-sized PCR product was amplified with all three primer pairs from EDL933, DEC3A-E, and DEC4A-E (FIG. 9). Appropriately sized products were obtained with primer pairs 846-1773 and 1774-2798 for DEC5 A, C, and E, but there were no products with primer pair 1-845 from these strains. It is possible that this region of stcE, which includes the putative promoter, is sufficiently different from stcE found on pO157 to prevent priming and amplification. DEC5B and D were negative for all three reactions.







TABLE 3







Incidence of stcE and its product in the DEC collection











DEC
Predominant
Disease
Number of
Number of


Number
Serotype
Category
stcE positive
StcE positive





 1A-E
O55:H6
EPEC
0/5
ND


 2A-E
O55:H6
EPEC
0/5
ND


 3A
O157:H7
EHEC
+
+


 3B
O157:H7
EHEC
+
+


 3C
O157:H7
EHEC
+
+


 3D
O157:H7
EHEC
+
+


 3E
O157:H7
EHEC
+
+


 4A
O157:H7
EHEC
+
+


 4B
O157:H7
EHEC
+
+


 4C
O157:H7
EHEC
+
+


 4D
O157:H7
EHEC
+
+


 4E
O157:H7
EHEC
+
+


 5A
O55:H7
EPEC
+
+


 5B
O55:H7
EPEC
−
−


 5C
O55:H7
EPEC
+
−


 5D
O55:H7
EPEC
−
−


 5E
O55:H7
EPEC
+
+


 6A-E
O111:H12
EPEC
0/5
ND


 7A-E
O157:H43
ETEC
0/5
0/5


 8A-E
O111:H8
EHEC
0/5
ND


 9A-E
O26:H11
EHEC
0/5
ND


10A-E
O26:H11
EHEC
0/5
ND


11A-E
O128:H2
EPEC
0/5
ND


12A-E
O111:H2
EPEC
0/5
ND


13A-E
O128:H7
ETEC
0/5
ND


14A-E
O128:H21
EPEC
0/5
ND


15A-E
O111:H21
EPEC
0/5
ND





Using a one kb probe internal to stcE, colony blot analyses were performed to determine which strains in the DEC collection contained stcE. Strains that were positive for the gene were checked for secretion of StcE as well proteolytic activity against C1-INH. Strains in bold contained the gene and produced the protein. Strains in italics contained the gene but lacked detectable protein. All other strains in the DEC collection were negative for stcE.


ND = not done.






Because previous experiments showed that StcE is released into the culture medium by EDL933 (FIG. 3), we examined whether the stcE-positive strains from the DEC collection also release StcE into the culture medium. We grew DEC3A-E, DEC4A-E, DEC5A-E, EDL933, and EDL933cu overnight in Lennox L broth at 37° C., harvested and concentrated the culture supernatants 100-fold. By immunoblot analysis we were able to detect StcE-reactive antigen in the supernatants of all stcE-positive strains and DEC5C (FIG. 10). The intensity of the reactive band varied from strain to strain and seemed to be stronger in the DEC3 group. To test if the bacterial-conditioned culture supernatants contained C1-INH proteolytic activity, we mixed purified C1-INH with the supernatants overnight and examined substrate cleavage by immunoblot. Again, all stcE-positive strains except DEC5C were able to degrade C1-INH (FIG. 11). Interestingly, DEC5B converted C1-INH from a single band to a doublet; this is unlikely to be related to StcE activity and the significance of this is unknown. It appears that DEC5C is unable to release StcE into the culture medium, although it contain stcE-like DNA. This may be due to a lack of expression of the gene or release of the protein from the cell.
II. Potentiation of C1-INH by StcE
Bacterial Strains, Buffers, and Materials.
All chemicals were purchased from Sigma (St. Louis, Mo.) unless otherwise indicated. Buffers used were PBS (10 mM sodium phosphate, 140 mM NaCl, pH 7.4), TBS (20 mM Tris, 150 mM NaCl, pH 7.5), VBS (5 mM veronal, 145 mM NaCl, pH 7.4), VBS2+ (VBS containing 0.15 mM CaCl2 and 0.5 mM MgCl2), and VBS containing 10 mM EDTA. Recombinant DNA manipulations were performed by standard methods. pTB4 was constructed by amplifying bases 207-2795 of the stcE gene from pO157 by PCR with the primers stcESac5′207 (5′-CCGAGCTC-CGGCTGATAATAATTCAGCCATTTATTTC-3′) (SEQ ID NO:20) and stcE3′Xba2795 (5′-CCTCGAGTTTATATACAACCCTGATTG-3′) (SEQ ID NO:21) and cloning the product into the SacI-XhoI sites of pET24d(+) (Novagen, Madison, Wis.). WAM2751 is E. coli strain BL21(DE3) (Novagen) transformed with pTB4. pTB5 was constructed in the same manner as pTB4 by amplifying the equivalent bases of the stcE E435D mutant from pTEG1, as described above. WAM2804 is E. coli strain BL21(DE3) transformed with pTB5. StcE′-His, lacking the StcE N-terminal signal sequence and containing a 6×His tag at the C-terminus, and StcE′ E435D-His, a similar protein with a single amino acid change at residue 435 (glutamic to aspartic acid) that disrupts the catalytic activity of the protease, were purified from WAM2751 and WAM2804, respectively, as described above and dialyzed into VBS, pH 6.5. Purified C1-INH was obtained from Advanced Research Technologies (San Diego, Calif.) and both purified kallikrein and C1s from Calbiochem (San Diego, Calif.). All proteins were stored at −80° C. Monoclonal antibodies (mAbs) 3C7 and 4C3 were generous gifts from Dr. Philip A. Patston (University of Illinois at Chicago, Ill.).
Hemolytic Assays
Sheep erythrocytes were prepared according to Mayer (42). Erythrocytes were opsonized with an anti-sheep red blood cell Ab for 10 min prior to use. Human serum (0.5%) was mixed with opsonized erythrocytes (1×107 in 50 μl) in VBS2+ to a total volume of 200 μl for one hour at 37° C. before the addition of one ml VBS+10 mM EDTA to stop complement activity. To measure the amount of hemoglobin released by lysed cells, erythrocytes were pelleted and the O.D.412 of the supernatant was measured in a spectrophotometer. The percent lysis was determined by subtracting the O.D.412 in the absence of serum and dividing by the maximum possible O.D.412 obtained by lysis of erythrocytes in water. Where indicated, increasing concentrations of StcE′-His or bovine serum albumin (BSA) were incubated with serum overnight at room temperature before the start of the assay. To determine the effect of StcE-treated C1-INH on erythrocyte lysis, increasing concentrations of StcE′-His or BSA were mixed with C1-INH (16 μg), or increasing concentrations of C1-INH were mixed with StcE′-His (one μg) or StcE′ E435D-His (one μg) in a total volume of 149 μl VBS2+ overnight at room temperature before the start of the assay. Statistical analyses were performed by the unpaired t test.
Flow Cytometry
C1-INH (8 μg) was untreated or treated with StcE′-His (one μg) in a total volume of 149 μl VBS2+ as described above before the addition of opsonized sheep erythrocytes and human serum deficient in complement component C5 (Quidel, San Diego, Calif.). Erythrocytes were incubated for 10 minutes at 37° C. before the addition of VBS+10 mM EDTA. Cells were washed with VBS2+ and incubated on ice for 30 min with polyclonal goat-anti-human IgG against C1-INH (Cedarlane Laboratories). Erythrocytes were washed, incubated on ice for 30 min with FITC-conjugated rabbit-anti-goat IgG, and resuspended in VBS2+ for analysis by flow cytometry using a fluorescence-activated cell sorter (FACSCalibur, Becton Dickinson, San Jose, Calif.). Where indicated, StcE′-His was removed from the assay mixture by adsorption to Ni-NTA agarose beads (Qiagen, Valencia, Calif.) in the presence of 50 mM imidazole prior to the addition of sheep erythrocytes. To measure StcE-treated C1-INH saturation kinetics of erythrocytes, increasing concentrations of C1-INH were mixed with StcE′E435D-His (one μg) before the addition of sheep erythrocytes (1×107) as described above in the absence of human serum and analyzed by flow cytometry.
To determine if StcE binds sheep erythrocytes, StcE′-His was labeled via its primary amines with the Alexa Fluor 488 dye as described by the manufacturer (Molecular Probes, Eugene, Oreg.). Sheep erythrocytes (5×106) were opsonized as described above, pelleted, and resuspended in 500 μl VBS2+ before the addition of StcE′-His or Alexa-labeled StcE′-His (250 ng) for 10 minutes at 37° C. Erythrocytes were pelleted and washed with VBS2+ before analysis by flow cytometry. To measure the point at which erythrocytes become saturated with StcE, increasing concentrations of Alexa-labeled StcE′-His were added to sheep erythrocytes (1×107) for 10 minutes at 37° C. before analysis by flow cytometry as described above.
Immunoblot Analyses
Purified, activated C1s (1.5 μg) was untreated, treated with C1-INH (100 ng), or treated with C1-INH in the presence of StcE′-His or StcE′ E435D-His (50 ng each) for one hour at 37° C. in a total volume of 30 μl VBS2+. An equal volume of non-reducing sample buffer was then added, the samples were heated to 95-100° C. for 5 minutes, and the proteins separated on an 8% SDS-PAGE gel. Proteins were transferred to nitrocellulose and analyzed by immunoblot as described using a polyclonal goat anti-C1s Ab (Calbiochem).
In other experiments, mAb 4C3 was coupled to Protein A-sepharose beads as described (43) and used to remove trace amounts of reactive center loop (RCL)-cleaved C1-INH from the purified C1-INH preparation. Virgin C1-INH (one μg) was then incubated with or without StcE′-His (one μg) or kallikrein (2 μg) for 18 hours at room temperature before electrophoresis on an 8% reducing SDS-PAGE gel. Separated proteins were transferred to nitrocellulose and analyzed by immunoblot as described using a polyclonal anti-human C1-INH Ab (Serotec, Raleigh, N.C.), mAb 3C7, or mAb 4C3.
Cell Culture
COS-7 cells (a gift from Dr. Donna Paulnock, University of Wisconsin, Madison, Wis.) were cultured in DMEM (Invitrogen, Carlsbad, Calif.) with 10% heat-inactivated fetal calf serum (FCS) (Mediatech, Herndon, Va.), non-essential amino acids, and penicillin/streptomycin/amphotericin B (Invitrogen). Cells were transfected with either hC1-INH/pcDNA3.1(−) or C-serp(98)/pcDNA3.1(−) (generous gifts from Dr. Alvin E. Davis, Harvard University, Cambridge, Mass.) using cationic lipids (Lipofectamine PLUS, Invitrogen). After transfection, cells were cultured in the presence of G418 (Invitrogen). Recombinant proteins were metabolically labeled with [35S]-methionine (Amersham Biosciences, Piscataway, N.J.) for 24 hours before immunoprecipitation.
Immunoprecipitation
Culture supernatants (100 μl) from C1-INH-transfected COS-7 cells were treated with StcE′-His (10 μg) overnight at room temperature before incubation with polyclonal goat anti-human C1-INH IgG (Cedarlane Laboratories, Ontario, Canada) and Protein A-sepharose (2 hours, room temperature). Pellets were washed three times with TBS, resuspended in sample buffer, and electrophoresed on 10% reducing SDS-PAGE gels. Gels were fixed, dried, and visualized with a phosphorimager (Typhoon 8600, Amersham Biosciences). In other experiments, C1-INH (5 μg) was untreated or treated with StcE′-His or StcE′ E435D-His (5 μg each) for 10 minutes at 37° C. in 500 μl of buffer (100 mM Tris, pH 8.0) before the addition of polyclonal goat anti-human C1-INH IgG. The mixture was rotated for 30 minutes at 4° C., after which 20 μl of a Protein A-sepharose slurry was added for 2 hours. The Protein A-sepharose beads were subsequently washed three times in buffer before immunoblot analysis with an anti-StcE Ab.
Kallikrein Activity Assay
Increasing concentrations of C1-INH were mixed with StcE′-His (250 ng) in a total volume of 100 μl assay buffer (50 mM Tris, pH 8.0, 100 mM NaCl) at room temperature overnight after which EDTA was added to 5 mM to stop the reaction. Purified kallikrein was diluted to 100 ng in 50 μl assay buffer and mixed with C1-INH for one hour at 37° C. before adding the chromogenic substrate S-2302 (Chromogenix, Milan, Italy) to each tube. Tubes were incubated at room temperature for 30 minutes before determining the absorbance of the substrate at 410 nm in a spectrophotometer. Percent kallikrein activity was determined by subtracting the O.D.410 in the absence of kallikrein and dividing by the maximum possible O.D.410 obtained by kallikrein activity in the absence of C1-INH.
Serum Resistance
C1-INH (8 μg) was untreated or treated with StcE′-His (one μg) in a total volume of 176 μl VBS2+ overnight at room temperature, after which human serum was added to 2%. E. coli K-12 strain C600 was grown to an O.D.595 of 0.5 in LB broth at 37° C. with aeration before being washed once and resuspended with an equivalent volume of VBS2+. Bacteria (20 μl) were added to the reactions, incubated at 37° C. for one hour, and 10 μl aliquots were mixed with VBS+10 mM EDTA to stop complement activity. Ten-fold serial dilutions of bacteria were plated on LB agar and percent survival was determined by dividing CFUs by the total number of bacteria after one hour in the absence of serum. Statistical analysis was performed by the unpaired t test.
Inhibition of Classical Complement-mediated Erythrocyte Lysis by StcE
Previous research from our laboratory demonstrated that StcE, a metalloprotease secreted by E. coli O157:H7, cleaves the serum protein C1-INH from its apparent Mr of 105 kDa to produce  ̃60-65 kDa species. Because C1-INH is an essential regulator of the classical complement pathway, we examined the effect of StcE on the classical complement-mediated lysis of sheep erythrocytes. Human serum was mixed overnight with increasing concentrations of StcE or the control protein BSA before adding to opsonized sheep erythrocytes for one hour at 37° C. The reaction was stopped with EDTA and the amount of hemoglobin released by lysed erythrocytes into the supernatant was measured.
Serum alone lysed 75.0% (±2.7% SEM) of erythrocytes, and BSA had no effect on the ability of serum to lyse erythrocytes (FIG. 12). At higher concentrations, StcE significantly reduced classical complement-mediated erythrocyte lysis compared to equivalent amounts of BSA (2 μg, p<0.01; 4 μg, p<0.005).
StcE Potentiates C1-INH-mediated Inhibition of Classical Complement
Although StcE cleaves the serum component C1-INH, the data represented in FIG. 12 does not implicate the serpin directly in StcE-mediated classical complement inhibition. To examine if StcE-treated C1-INH plays a role in this process, we mixed purified C1-INH with increasing concentrations of StcE or BSA overnight and added this mixture to human serum and opsonized sheep erythrocytes for one hour at 37° C. As before, the reaction was stopped with EDTA and the amount of hemoglobin released by lysed erythrocytes into the supernatant was measured. The addition of 0.4 IU untreated C1-INH to the assay decreased erythrocyte lysis from 82.9% (±1.1% SEM) to 33.7% (±1.8% SEM) (FIG. 13A), demonstrating the effective role of C1-INH in the inhibition of classical complement activity. Whereas BSA-treated C1-INH was unchanged in inhibitory activity, 0.4 IU StcE-treated C1-INH reduced erythrocyte lysis to between 1.5% (±0.5% SEM) and 0.1% (±0.1% SEM) of total.
To confirm the effect of StcE on C1-INH-mediated inhibition of erythrocyte lysis, increasing concentrations of C1-INH were untreated or treated with StcE′-His overnight prior to the addition of human serum and opsonized sheep erythrocytes. Increasing concentrations of untreated C1-INH (0.05 to 0.4 IU) resulted in a dose-dependent decrease in erythrocyte lysis, ranging from 76.5% (±2.4% SEM) to 27.5% (±1.1% SEM) (FIG. 13B). However, in the presence of one μg StcE′-His, the same concentrations of C1-INH significantly reduced lysis below that of untreated C1-INH (ranging from 46.7% (±1.5% SEM) lysis to 2.8% (± ̃1.3% SEM) lysis, all p values <0.0005). These results demonstrate the direct role of StcE-treated C1-INH in the decrease of classical complement-mediated erythrocyte lysis. Additional experiments confirm that StcE-treated C1-INH continues to react with its natural targets C1r and/or C1s to mediate the inhibition of classical complement, maintaining the target specificity of the serpin (data not shown).
StcE Binds Erythrocyte Surfaces
In order to understand how StcE might potentiate C1-INH, we asked if StcE could interact with erythrocytes, thereby acting as a binding protein for C1-INH on cell surfaces. Unlabeled StcE′-His or a form of StcE′-His fluorescently labeled via its primary amines with the Alexa Fluor 488 dye (Alexa-StcE′-His) (250 ng each) were added to opsonized sheep erythrocytes for 10 minutes at 37° C. Erythrocytes were pelleted and washed with VBS2+ before analysis by flow cytometry. Erythrocytes treated with Alexa-labeled StcE′-His showed 80-fold greater mean fluorescence compared to cells treated with unlabeled StcE′-His (FIG. 14A), demonstrating a direct interaction between these cells and the protease. Furthermore, we observed that StcE continues to bind sheep erythrocytes even at lower temperatures (0-4° C.), suggesting that this interaction is not mediated by an active cellular process (data not shown). To determine if the interaction between erythrocytes and StcE is specific and therefore saturable, we mixed increasing concentrations of Alexa-labeled StcE′-His with sheep erythrocytes (1×107) as described above. We observed that this number of erythrocytes becomes saturated with StcE′-His at 3.2 μg of the protease in 500 μl (FIG. 14B). Based on the calculated molecular weight of StcE′-His, at this concentration we estimate approximately 1.8×106 molecules of StcE are bound per erythrocyte.
StcE Localizes C1-INH to Erythrocyte Surfaces
Based on the ability of StcE to directly bind erythrocytes, we asked if StcE could localize C1-INH to erythrocyte surfaces, thereby possibly increasing the local concentration of inhibitor at the site of potential lytic complex formation. C1-INH was untreated or treated with StcE′-His overnight before the addition of opsonized sheep erythrocytes and C5-deficient human serum (to prevent formation of the membrane attack complex and lysis of the cells) for 10 min at 37° C. Erythrocytes were washed, treated with an Ab against C1-INH and washed again prior to the addition of a FITC-conjugated secondary Ab. Deposition of C1-INH was subsequently analyzed by flow cytometry. Little to no C1-INH binding was measured on erythrocytes treated with only StcE′-His or native C1-INH compared to mock-treated cells (FIG. 15A). Increased deposition of C1-INH was detected on erythrocytes mixed with 0.2 IU StcE-treated C1-INH, however. Similar results were observed in the absence of human serum, indicating that serum components or complement activation are not required for the localization of StcE-treated C1-INH to erythrocyte surfaces. As we observed with the interaction between StcE and sheep erythrocytes, StcE-treated C1-INH continues to bind the cells at 0-4° C. (data not shown).
The results suggest that StcE may directly mediate the binding of C1-INH to the cell surface. To test this possibility, C1-INH was incubated with or without StcE′-His overnight as described earlier, following which Ni-NTA agarose beads were added to the samples in the presence of 50 mM imidazole to specifically remove the 6×His-tagged StcE protein from the assay. The agarose beads were pelleted and the supernatants were mixed with sheep erythrocytes and analyzed by flow cytometry as described above. In the absence of the protease, StcE-treated C1-INH no longer binds to erythrocyte surfaces (FIG. 15B), indicating that StcE itself is required to sequester C1-INH to erythrocytes. The absence of StcE′-His from the Ni-NTA agarose-treated samples and the presence of equivalent amounts of C1-INH between Ni-NTA agarose-treated and untreated samples were confirmed by immunoblot analyses (data not shown).
To determine the level at which erythrocytes become saturated with StcE-treated C1-INH, we mixed StcE′ E435D-His (a mutant form of the protein containing a single amino acid change from glutamic to aspartic acid at position 435 that is unable to cleave C1-INH with increasing concentrations of C1-INH (from 0.25-16 μg) before the addition of opsonized sheep erythrocytes as described above. We chose to use StcE′ E435D-His in this experiment so as to measure the saturation of sheep erythrocytes with C1-INH without the creation of StcE-cleaved C1-INH, which might reduce the levels of the serpin bound to the cell surface, thereby increasing the amount needed to saturate the cells. We observed that, in the presence of one μg StcE′E435D-His, this number of erythrocytes becomes saturated with C1-INH at 4 μg, or 0.1 IU, of the serpin (FIG. 15C). Based on the observed molecular weight of mature C1-INH and assuming uniform binding of the primary and secondary antibodies to their antigens, at this concentration we estimate approximately 2.25×106 molecules of C1-INH are bound per erythrocyte. Finally, to determine if C1-INH and StcE can interact in solution (prior to binding erythrocytes), C1-INH was mixed with either StcE′-His or StcE′ E435D-His for 10 min at 37° C. before immunoprecipitating the mixture with an anti-C1-INH Ab. After separating the immunoprecipitated proteins by SDS-PAGE and transferring them to nitrocellulose, both StcE′-His and StcE′ E435D-His were detected with an anti-StcE′-His Ab, demonstrating that a complex of StcE and C1-INH can be formed in solution (FIG. 15D).
Cleavage of C1-INH by StcE is not Necessary to Protect Cells Against Complement Activity
To test if the proteolysis of C1-INH by StcE is necessary to provide erythrocytes increased protection against classical complement activity over that of untreated C1-INH, we mixed C1-INH with either StcE′-His or the proteolytically inactive StcE′ E435D-His. After overnight incubation, the samples were added to human serum and opsonized sheep erythrocytes for one hour at 37° C. before determining the amount of hemoglobin released into the supernatant by the lysed cells as described above. In the presence of 0.2 IU C1-INH, human serum lysed 75.8% (±1.5% SEM) of the erythrocytes, whereas StcE′-His-treated C1-INH significantly decreased erythrocyte lysis to 25.4% (±3.5% SEM, p<0.005) (FIG. 16A). Interestingly, the cleavage of C1-INH by StcE is not required for the protection of erythrocytes from complement activity, as StcE′ E435D-His-treated C1-INH was able to significantly reduce the lysis of the cells to 16.7% (±1.3% SEM, p<0.005). The difference in the extent of erythrocyte lysis between the StcE′-His-treated and the StcE′ E435D-His-treated C1-INH samples was not significant (p>0.05).
To determine if the subsequent cleavage of C1-INH by StcE affects the binding of the serpin to erythrocytes, we incubated C1-INH (2 μg) with StcE′-His or StcE′ E435D-His (one μg each) overnight before assessing the levels of surface-associated C1-INH by flow cytometry as described above. Indeed, the levels of C1-INH in the presence of StcE′ E435D-His on erythrocyte surfaces are 22-fold higher than in the presence of StcE′-His (FIG. 16B), suggesting that, cleaved C1-INH binds erythrocytes less efficiently than intact C1-INH. In total, the data presented in FIGS. 4 and 5 demonstrate that the increased protection of erythrocytes by StcE-treated C1-INH is dependent upon the physical presence of StcE and not the cleavage of C1-INH by the protease.
StcE is Unable to Potentiate C1-INH in the Absence of Cells
Data presented so far demonstrate the ability of StcE to localize C1-INH to erythrocytes, thereby providing increased complement-inhibiting activity at the cell surface. To determine if this potentiation can occur in solution (i.e. in the absence of cells), we measured whether StcE affects the ability of C1-INH to inhibit kallikrein, another C1-INH-regulated molecule, by monitoring the cleavage of a chromogenic substrate of kallikrein, S-2302 (H-D-Prolyl-L-phenylalanyl-L-arginine-p-nitroaniline dihydrochloride). Increasing concentrations of C1-INH were untreated or treated with StcE′-His overnight at room temperature, after which the samples were allowed to react with kallikrein for one hour at 37° C. The C1-INH/kallikrein mixtures were subsequently incubated with S-2302 for 30 min at room temperature before determining total kallikrein activity by measuring the change in absorbance of the samples in a spectrophotometer. For the purpose of this assay, kallikrein in the absence of C1-INH was considered to be 100% active. As expected, increasing concentrations of C1-INH resulted in a dose-dependent decrease in kallikrein activity, ranging from 88.8% (±2.6% SEM) to 10.3% (±1.3% SEM) activity (FIG. 17A). The addition of StcE-treated C1-INH to the assay did not significantly alter the inactivation of kallikrein compared to untreated C1-INH.
We also examined the ability of StcE-treated C1-INH to interact with an excess of C1s in solution, thereby forming an SDS-insoluble complex. Purified, activated C1s (1.5 μg) was mixed with C1-INH (100 ng) and StcE′-His or StcE′ E435D-His (50 ng each) for one hour at 37° C. before separating the proteins by non-reducing SDS-PAGE and analyzing the mixture by immunoblot with an anti-C1s Ab. The high molecular weight band in samples containing C1-INH that are absent from the sample containing C1s alone are indicative of the C1s-C1-INH interaction. If StcE were able to potentiate C1-INH in solution, an increase in the intensity of the C1s-C1-INH band would be visible; however, this does not appear to be the case (FIG. 17B). Thus, the mechanism of StcE-mediated potentiation of C1-INH is dependent on the presence of cell surfaces upon which the protease-serpin complex can bind.
Interaction of StcE with the N-terminus of C1-INH
The ability of StcE to interact with C1-INH while maintaining the inhibitory activity of the molecule suggests that StcE may bind C1-INH in the heavily glycosylated N-terminal domain, leaving the serpin domain unaffected. Therefore, to further characterize the site(s) of cleavage by StcE, we examined StcE-treated C1-INH with the monoclonal antibodies 3C7 and 4C3, directed against the amino terminus of C1-INH (44) and the RCL-inserted form of C1-INH (45), respectively. As most preparations of purified C1-INH contain trace amounts of RCL-cleaved C1-INH, we removed this species of C1-INH from the mixture by immunoprecipitation with mab 4C3 prior to analysis. Virgin C1-INH was treated with StcE or kallikrein, a serine protease inactivated by C1-INH via its interaction with and cleavage of the RCL, prior to analysis by immunoblot with a polyclonal anti-human C1-INH Ab (FIG. 18A, left panel), 3C7 (FIG. 18A, middle panel), or 4C3 (FIG. 18A, right panel). As expected, analysis with 3C7 detected both virgin C1-INH and kallikrein-reacted C1-INH, but did not detect StcE-cleaved C1-INH, indicating a modification of the C1-INH N-terminus by StcE. Additionally, analysis with 4C3 detected RCL-inserted C1-INH produced upon interaction with kallikrein, but not virgin C1-INH or StcE-treated C1-INH.
To confirm that StcE interacts with the N-terminal domain of C1-INH, we examined the ability of StcE to cleave a recombinant C1-INH protein lacking this region. Coutinho et al. demonstrated that C-serp(98), a recombinant C1-INH molecule lacking the N-terminal amino acids 1-97 and containing only the serpin domain, binds its serine protease substrates identically to wild-type C1-INH and is effective in inhibiting C1 activity in hemolytic assays (31). We expressed recombinant, full-length human C1-INH (hC1-INH) and C-serp(98) in COS-7 cells and harvested the culture media after 24 hours in the presence of [35S]-methionine. Samples were untreated or treated with StcE′-His overnight before immunoprecipitating metabolically labeled protein with polyclonal anti-human C1-INH IgG. Both hC1-INH and C-serp(98) migrated at the appropriate molecular weights on a reducing SDS-PAGE gel, however, only hC1-INH was cleaved by StcE′-His; C-serp(98) was unaffected by the protease (FIG. 18B). These analyses further support the evidence that StcE does not inactivate C1-INH, but instead interacts with the heavily glycosylated N-terminal domain of C1-INH, leaving the serpin domain available for interaction with C1-INH targets.
Increased Bacterial Serum Resistance in the Presence of StcE-treated C1-INH
StcE is secreted by E. coli O157:H7, a human pathogen that may come in contact with blood or blood products during the course of an infection. Based on its ability to enhance C1-INH-mediated inhibition of classical complement, we examined if StcE could provide serum resistance to E. coli. As E. coli O157:H7 is naturally serum resistant and contains a variety of factors that could contribute to its protection from complement (1), we chose to assess the role of StcE-treated C1-INH in the survival of a serum-sensitive strain of E. coli. E. coli K-12 strain C600 was grown to mid-log phase, pelleted, and resuspended in an equivalent amount of VBS2+ before the addition of human serum and 0.2 IU C1-INH or StcE-treated C1-INH. Bacteria were incubated at 37° C. for one hour, serially diluted and plated onto LB agar to determine the numbers of surviving CFUs. In the presence of human serum alone, 0.07% (±0.06% SEM) of bacteria survived, demonstrating the exquisite serum sensitivity of E. coli strain C600 (FIG. 19). The addition of StcE′-His to bacteria at the beginning of the assay had no significant effect on survival (0.04% survival, ±0.03% SEM). As expected, the addition of untreated C1-INH increased survival of bacteria to 3.9% (±0.9% SEM). The addition of StcE-treated C1-INH to the assay, however, caused a significant increase in bacterial survival over untreated C1-INH (16.5% survival, ±1.9% SEM; p<0.005), indicating a contribution to complement resistance by StcE.
III. Further Characterization of StcE and its Role in Pedestal Formation Construction of StcE Knockout and Restored Strains
Construction of EDL933 ΔstcE::cm (WAM 2815)
Construction of EDL933 ΔstcE::cm (WAM 2815). A deletion mutant of stcE from EDL933 was constructed by the linear recombination (λ Red) method of Datsenko and Wanner (77). Briefly, the oligonucleotides 5′ 707 (5′-ATGAAATTAAAGTATCTGTCATGTACGATCCTTGCCCCTTGTGTAGGCTGGAGC TGCTTC-3′) (SEQ ID NO:22) and 3′ 708 (5′-TAATTTATATACAACCCTCATTGACCTAGGTTTACTGAAGCATATGAATATCCTCCTTAG-3′) (SEQ ID NO:23) were used to amplify by PCR the chloramphenicol resistance cassette from the non-polar (in frame, with added ribosome binding site) plasmid template pKD3. The resulting product was then transformed by electroporation into WAM2806 (EDL933 carrying pKD46, grown at 30° C. in the presence of 10 mM arabinose). Transformants cured of pKD46 and lacking the stcE coding sequence were selected by growth on LB agar containing chlormaphenicol (20 μg/ml) at 42° C. and were confirmed by PCR. More than 95% of the coding sequence of stcE was deleted, leaving behind the 5′ and 3′ ends of the gene encoded by the oligonucleotides.
A StcE complemented strain having the stcE gene restored was created using a Tn7 transpose system similar to that described by DeLoney et al. (77). The stcE gene was PCR amplified using primers 5′1135 (5′-AAG GGC CCC TCT GAG GTG TCT GTT AAA CCC GTG G-3′) (SEQ ID NO:24) and 5′1136 (5′-AAA AA TGG CCA CGA AGT GGC CGC ACC GTC TCA GG-3′) (SEQ ID NO:25). The gene was put into the ApaI-MscI sites of pEVS107 and electroporated into the mating strain WAM1301, E. coli 517λpir, creating a strain called WAM2980. The strains WAM2980, WAM2815, and WAM2871 (E. coli carrying the helper plasmid pUX-BF13 which has the Tn7 transpose genes tnsABCDE), were mated. This resulted in strain WAM2997, which is the WAM2815 ΔstcE::cm strain that carries a single copy of stcE on the chromosome and has restored StcE expression.
Production of an Untagged, Purified StcE Protein
Recombinant, untagged StcE protein was created using the IMPACT protein expression system from NEB (New England Biolabs, Beverly, Mass.). Briefly, the stcE gene was amplified by PCR using Deep Vent polymerase (NEB) and purified pO157 plasmid DNA as a template. The gene was then inserted into pTYB1 (NEB) at the NheI and SapI restriction sites of the multiple cloning site, creating pTEG11. This plasmid created a fusion gene of stcE with sequences encoding a chitin binding domain and an intein protease. The plasmid was moved into the expression strain ER2566 (NEB). The chitin-binding domain of the expressed protein allowed affinity purification on a chitin-sepharose column, while the intein protease allows the target protein to be released from the two fusion domains. The result was a StcE protein that had three extra residues (Met-Ala-Ser) at its N-terminus, but is otherwise identical to StcE secreted from strains carrying the pO157 plasmid. This same protocol can be used to express a proteolytically inactive form of rStcE called E435D, which has a mutation of a glutamate to aspartate residue at the zinc metalloprotease.
Tissue Culture of HEp-2 Cells
HEp-2 cells, which are derived from a contaminant of the human cervical epithelial cell line HeLa, were maintained in Eagle's modified medium (Mediatech Herndon, Va.) supplemented with 10% FBS (Atlanta Biologicals, Norcross, Ga.), 10 mM sodium pyruvate, penicillin, streptomycin, and amphotericin B. Cells were passed after achieving confluence by lifting with 0.25% Trypsin-EDTA (Mediatech) and diluting to 1:5 or 1:10.
Microscopic Analysis of Pedestal Formation
Evaluation of pedestal formation was adapted from Knutton et al. Infection & Immunity 57: 1290 (1989) and Methods in Enzymology 253: 324.
Bacterial strains were inoculated from a single colony on an LB plate into Lennox broth (2 ml) at 37° C., without shaking and grown to stationary phase (12-18 hours). The media used for infection was Dubelco's modified MEM medium supplemented as above. The overnight bacterial culture was diluted 1:25 in this media, for an average inoculation of 8×106 CFU/ml. HEp-2 cells from a confluent dish were diluted 1:5 or 1:10 and grown in 4 or 8 well slides (Nalge Nunc International, Naperville, Ill.) for 24 to 48 hours (50-80% confluent). Cells were washed three times in PBS to remove any residual antibiotics in the media. Then the DMEM-bacterial mixture was placed on the cells, 0.5 ml for 4 well slide, 0.25 ml for 8 well slide, and incubated at 37° C. for 6-7 hours, with or without simultaneous addition of recombinant StcE (2 μg). Media was removed at midpoint of incubation and replaced with fresh media and no additional bacteria.
After infection, cells were washed thoroughly (5-6 times) with PBS and fixed in paraformaldhehyde (3% for 10 minutes). Cells were washed with PBS (three times for two to three minutes) after fixation. A 15 minute blocking step with antibody dilution solution (2% BSA, 0.1% Triton X-100 in PBS) was used to inhibit non-specific staining. Bacteria were stained with a monoclonal antibody recognizing the O157 antigen (US Biologicals, Swampscott, Mass.) for 30 minutes in a 1:200 dilution. After washing in PBS, cells were stained with the secondary antibody solution containing goat anti-rabbit conjugated to Alexa488, 1:1000 (Molecular Probes, Netherlands), and Phalloidin conjugated to Alexa594 1:400 (Molecular Probes).
Samples were analyzed using a Zeiss (Carl Zeiss MicroImaging Inc., Thornwood, N.Y.) fluorescent microscope using a 40× plan apochromat NA 1.3 objective. Images were acquired with a Axiocam monochrome CCD camera and Openlab software (Improvision, Lexington, Mass.). Random fields from blinded sample wells were selected out-of-focus on the phalloidin channel such that no actin pedestals were discernible that might bias selection. Number of foci that had formed pedestals were counted in each field after 10 (8 well slides) or 20 (4 well slides) fields were imaged of each sample. Foci were defined as either a single bacterium or a cluster of bacteria separated from other foci by more than the length of a bacterium.
The results indicate that the StcE knockout mutant has a lower capacity for forming pedestals than the wild type or the complemented strain, but the ability is restored by supplementation with exogenous StcE (FIG. 20).
StcE Reduces Viscosity of Saliva and Solubilizes Mucus
Because StcE has the capacity to cleave a heavily glycosylated, mucin-like region of C1-INH, studies were undertaken to determine whether StcE was capable of cleaving other substrates. Human saliva and sputum, which are good sources of mucins and glycoproteins, were evaluated as follows.
Whole human saliva was collected and split into 10 ml samples. The viscosity of the samples was measured before and after a 3 hour treatment with rStcE or buffer. Both the treatment and measurements took place at 37° C. Relative viscosity was measured in a Cannon-Fenske Routine Viscometer (range: 0.5 to 4 cP) (Cannon Instrument Company, State College, Pa.), and elution time was measured, with less viscous solutions that travel through the viscometer relatively quickly having shorter elution times than more viscous solutions. Treatment with StcE was found to reduce the viscosity of saliva by about 66% (FIG. 21).
Mucus from a person with a cold was expectorated into a sample tube. Aliquots (1 ml) were transferred into a 10 ml glass tube using a blue tip (made for a p-1000 pipette) with the tip cut off, creating a wider bore. One tube was untreated and to the other StcE (40 μg) was added. Both were vortexed briefly (setting 4 of 9) and incubated 3 days at room temperature. The samples were then vortexed briefly and tested for ability to be pipetted by a blue tip with a 1000 μl pipet and for ability to flow freely in the 10 ml sample tube. The StcE treated sample was free-flowing and easily pipetted while the untreated sample was still thick and viscous, and would get stuck in a blue tip.
Identification of Saliva Proteins Cleaved by StcE
Whole human saliva was collected and treated with rStcE (1 μg) or untreated for one hour at room temperature. Proteins were separated by SDS-PAGE (8% acrylamide) and stained with Coomassie R-250. With reference to FIG. 22, lane 1 includes rStcE-His; lane 2 includes untreated saliva; and lane 3 contains saliva treated with rStcE-His. Proteins of interest (indicated with arrows) were excised from the gel, digested with trypsin and analyzed by MADLI-TOF mass spectrometry. Peptide masses were compared to the ProteinProspector database (University of California at San Francisco) for identification. The proteins were found to be MUC7 and gp-340/DBMT1.
ELISA for C1-INH Cleavage by StcE
An enzyme-linked immunosorbant assay (ELISA) was used to test for StcE protease activity toward C1-INH in a 96 well format. The primary antibody 3C7 (gift of Phil Patston, University of Illinois at Chicago) binds only uncut C1-INH, but does not bind to either fragment produced by StcE cleavage. The antibody is thought to bind somewhere in the N-terminal 100 amino acids of C1-INH, but the binding site is presumed to be destroyed by StcE cleavage.
First, 0.1 to 10 mmol C1-INH was mixed with 0-5 mmol StcE in a total volume of 100 μl PBS (phosphate buffered saline) in a round bottom microtitre plate (Sarstedt). After 1 hour at 37° C., an EDTA solution (10 mM in PBS, 50 μl) was added to stop the reaction. The reaction mixtures were transferred to an ELISA plate (Dynatech flatbottom Immulon plates, Alexandria, Va.) and held at room temperature (RT) for 2 hours to allow binding of proteins to the plate. The wells were emptied, bovine serum albumin (BSA, 1% in PBS, 100 μl) was added to each well and incubated for 30 minutes to block non-specific binding. The wells were then washed three times for one minute each with PBST (PBS with 0.05% Tween-20, 200 μl). The primary antibody was diluted 1:1500 in blocking solution and 100 μl was added to each well and incubated at RT for 60 minutes. Wells were again washed, and then the secondary antibody (goat anti-mouse conjugated to horseradish peroxidase, BioRad, Hercules, Calif.) was diluted 1:3000 in blocking solution and 100 μl added to each well and incubated at RT for 30 minutes. After a final wash, substrate solution (100 μl TMB, BioRad) was added to each well. After a ten-minute incubation while rocking at RT, stop solution was added (100 μl IN sulfuric acid). Absorbance at 450 nm was read and used to calculate the C1-INH remaining in the wells. C1-INH remaining was plotted as a function of the amount of C1-INH added to the well (FIG. 24). The results indicate that the amount of detectable (i.e., intact) C1-INH is reduced in the presence of StcE.
This method provides a means of detecting the presence of StcE activity in a test sample. In addition, the method provides a means for evaluating the ability of a test substance to inhibit cleavage of C1-INH in the presence of StcE.
Proposed ELISA for Detection of StcE Protein in Mixtures or Clinical Samples
StcE is expressed in enterohemorrhagic E. coli infections, although it is presently known whether the protein is expressed early or late in the course of colonization and infection. If it expressed early, detection of StcE could be a valuable tool for early detection of infection.
A clinical sample such as a fecal filtrate or bacterial supernatant is adsorbed onto an untreated ELISA plate. After a block and wash step, the wells are probed with an antibody against the StcE protein. Following another wash, a secondary antibody (e.g., anti-mouse conjugated to horseradish peroxidase, BioRad) is added to the wells. After a final wash, a substrate solution, such as TMB substrate (BioRad), is cleaved by the horseradish peroxidase, and its product is detected, indicating the presence of StcE.
Alternatively, a sandwich ELISA method is employed in which the ELISA plate is be pre-adsorbed with antibodies to StcE. The subsequent steps are the same as those outlined in the preceding method.
Model of StcE Role in EHEC Colonization of Host Cells
We propose a model in which the StcE-mediated increase in pedestal formation is due to cleavage of proteins from the glycocalyx and/or cell surface, allowing a closer interaction of bacterium and host cell. Cleavage of these proteins may also lead to decreased adherence of normal flora, which would compete with EHEC for space and resources. Addition of StcE to cell extracts prepared from uninfected HEp-2 cells showed that StcE changed the banding pattern of proteins separated via SDS-PAGE. This data supports the model that host cell proteins are being cleaved or modified in a way that is favorable to intimate adherence by EHEC. It is expected that most of these proteins are cell-surface proteins, and identification of these potential substrates is underway.
StcE Reduces Viscosity of Cystic Fibrosis Sputum
Expectorated sputum samples collected for routine bacterial culture from Cystic Fibrosis (CF) patients were obtained (IRB approval granted). Specimens were divided into equal portions in 1.5 ml microcentrifuge tubes and treated with StcE (14 μg) in PBS, E435D StcE in PBS, or PBS alone. The samples were incubated at 37° C. and periodically inverted to observe viscosity. In 78% of CF sputum samples (n=18) treated with StcE, reduced viscosity was observed. In contrast, no change in viscosity was observed in sputum samples treated with E435D StcE or PBS. Reduced viscosity was generally observed 20-30 minutes post-treatment, and often the effect was enhanced by longer incubation, such as for 1-2 hours.
StcE Degrades gp340 and MUC7 in Cystic Fibrosis Sputum
Expectorated sputum samples collected for routine bacterial culture from CF patients were obtained (IRB approval granted). Samples were vortexed for 60 seconds in PBS to extract mucous material and centrifuged. Supernatants were treated with PBS, StcE, or E435D StcE at 37° C. for 60 minutes, then boiled in SDS-PAGE sample buffer and separated on 1% agarose gels in TAE+0.1% SDS. Gels were blotted to nitrocellulose membranes by capillary transfer and the proteins were probed with antibodies or antisera to gp340 (1:5000, mouse anti-human, MBL, Woburn, Mass.), MUC7 (1:1000, rabbit anti-human, gift of J. G. M. Bolscher), MUC5AC (1:500, mouse anti-human, U.S. Biological, Swampscott, Mass.), MUC5B (mouse anti-human, 1:500, U.S. Biological), and MUC2 (rabbit anti-human, 1:500, Biomeda, Foster City, Calif.), followed by secondary antibodies conjugated to horseradish peroxidase.
Immunoblots of protein extracts from cystic fibrosis sputum samples demonstrated that a band identified via monoclonal antibody against gp340 showed degradation following incubation with StcE. No degradation of gp340 was observed in samples incubated with buffer or E435D StcE. Antisera against MUC7 showed a breakdown of MUC7 following incubation with StcE, in one sample. No degradation of MUC7 was observed in samples incubated with buffer or E435D StcE. Antibodies against mucins commonly found in CF sputum secretions, including MUC5B, MUC5AC, and MUC2, were used to probe immunoblots of the samples. None of the bands recognized by the mucin antibodies was altered by StcE treatment.
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## Claims

1. A isolated polypeptide having at least 95% amino acid identity to SEQ ID NO:2 defective in proteolytic activity against C1 esterase inhibitor and capable of potentiating C1-INH protection against complement activity, wherein the polypeptide having a mutation in at least one amino acid corresponding to amino acid residues 434-444 of SEQ ID NO:2.

2. The polypeptide of claim 1, wherein the polypeptide comprises a mutation in a position corresponding to amino acid residue 435.

3. The polypeptide of claim 2, wherein the polypeptide comprises a mutation corresponding to E435D.

4. The polypeptide of claim 1, wherein the polypeptide comprises the sequence of SEQ ID NO:19.

5. A composition comprising the polypeptide of claim 1.