# Patent

## Title
Apoptosis-inducing polypeptides

## Abstract

An isolated water-soluble VP1 polypeptide of foot-and-mouth disease virus and a nucleic acid encoding the polypeptide. Also disclosed are a pharmaceutical composition containing the polypeptide or nucleic acid and related methods of inducing apoptosis and treating an apoptosis-related disorder.

## Background
RELATED APPLICATION
This application is a division of and claims priority to U.S. application Ser. No. 10/863,637, filed Jun. 8, 2004 and now issued as U.S. Pat. No. 7,323,546, which is a continuation-in-part of, and claims priority to, U.S. application Ser. No. 10/449,531, filed May 29, 2003 and now issued as U.S. Pat. No. 7,217,784, the contents of which are incorporated herein by reference.


BACKGROUND
Apoptosis, i.e., programmed cell death, is a normal physiological process of a cell, which is characterized by DNA fragmentation, cytoplasma shrinkage, membrane change, and cell death without damaging neighboring cells. This process is regulated by a combination of various extracellular and intracellular signals. It allows a multicelluar organism to replace aged cells, control the cell number and the tissue size, and protect itself from cells that may lead to lethality. See, e.g., Li et al., Science 302, 1560-1563. Impaired apoptosis results in excessive levels of unwanted cells, which, in turn, cause disorders such as cancers, autoimmune diseases, immunodeficiency diseases, reperfusion injuries, and neurodegenerative diseases. Therefore, apoptosis-inducing compounds are drug candidates for treating these disorders.
SUMMARY
This invention relates to an isolated water-soluble VP1 polypeptide of foot-and-mouth disease virus that can induce apoptosis. The full-length VP1 polypeptide and the nucleic acid encoding it (SEQ ID NOs: 1 and 2, respectively) are listed below:











 M  A  S  M   T  G  G   Q  Q  M   G  R  G  S   T  T  S
(SEQ ID NO: 1)



  1
ATGGCTAGCA TGACTGGTGG ACAGCAAATG GGTCGCGGAT CCACCACCTC
(SEQ ID NO: 2)


 



  A  G  E   S  A  D  P   V  T  A   T  V  E   N  Y  G  G


 51
TGCGGGTGAG TCTGCGGACC CCGTGACTGC CACCGTCGAG AACTACGGTG


 



   E  T  Q   V  Q  R   R  Q  H  T   D  S  A   F  I  L


101
GTGAGACACA AGTCCAGAGG CGCCAGGACA CGGACAGTGC GTTCATATTG


 



 D  R  F  V   K  V  K   P  K  E   Q  V  N  V   L  D  L


151
GACAGGTTCG TGAAAGTCAA GCCAAAGGAA CAAGTTAATG TGTTGGACCT


 



  M  Q  I   P  A  H  T   L  V  G   A  L  L   R  T  A  T


201
GATGCAGATC CCTGCCCACA CCTTGGTAGG GGCGCTCCTG CGAACGGCCA


 



   Y  Y  F   S  D  L   E  L  A  V   K  H  E   G  D  L


251
CCTACTACTT CTCTGACCTG GAGCTGGCCG TCAAGCACGA GGGCGATCTC


 



 T  W  V  P   N  G  A   P  E  T   A  L  D  N   T  T  N


301
ACCTGGGTCC CAAACGGCGC CCCTGAGACA GCACTGGACA ACACTACCAA


 



  P  T  A   Y  H  K  E   P  L  T   R  L  A   L  P  Y  T


351
CCCAACAGCT TACCACAAGG AACCCCTCAC ACGGCTGGCG CTGCCTTACA


 



   A  P  H   R  V  L   A  T  V  Y   N  G  S   S  K  Y


401
CGGCTCCACA CCGTCTCTTA GCGACCGTCT ACAACGGGAG CAGTAAGTAC


 



 G  D  T  S   T  N  N   V  R  G   D  L  Q  V   L  A  Q


451
GGTGACACCA GCACTAACAA CGTGAGAGGT GACCTTCAAG TGTTAGCTCA


 



  K  A  E   R  T  L  P   T  S  F   N  F  G   A  I  K  A


501
GAAGGCAGAA AGAACTCTGC CTACCTCCTT CAACTTCGGT GCCATCAAGG


 



   T  R  V   T  E  L   L  Y  R  M   K  R  A   E  T  Y


551
CAACTCGTGT TACTGAACTA CTCTACAGAA TGAAGAGAGC CGAGACATAC


 



  C  P  R  P   L  L  A   I  Q  P   S  D  A  R   H  K  Q


601
TGTCCCAGGC CCCTTCTCGC CATTCAACCG AGTGACGCTA GACACAAGCA


 



 R  I  V   A  P  A  K   Q  L  L   L  E  H   H  H  H  H


651
GAGGATTGTG GCACCCGCAA AACAGCTTCT GCTCGAGCAC CACCACCACC


 



  H


701
ACCAC






In one aspect, the invention features an isolated water-soluble VP1 polypeptide of foot-and-mouth disease virus that contains RGD (SEQ ID NO: 6). The polypeptide is 25 to 800 amino acids in length (i.e., any number between 25 and 800 amino acids, e.g., 29 and 235 amino acids, inclusive). In one embodiment, it contains RGDL (SEQ ID NO: 5) or NGSSKYGDTSTNNVRGDLQVLAQKAERTL (SEQ ID NO: 4). In a preferred embodiment, it contains the sequence of the full-length VP1 polypeptide listed above (SEQ ID NO: 1), the sequence of a mutant form that has a cysteine 201 to serine mutation (SEQ ID NO: 3), or the sequence of capsid polyprotein P1 listed below (VP4-1, SEQ ID NO: 7).








(SEQ ID NO: 7)









GAGQSSPTTGSQNQSGNTGSIINNYYMQQYQNSMDTQLGDNAISGGSNEG



 


STDTTSTHTNNTQNNDWFSKLANTAFSGLFGALLADKKTEETTLLEDRIL


 


TTRNGHTTSTTQSSVGVTYGYATAEDFVSGPNTSGLETRVVQAERFFKTH


 


LFDWVTSDPFGRCHLLELPTDHKGVYGSLTDSYAYMRNGWDVEVTAVGNQ


 


FNGGCLLVAMVPELCSISKRELYQLTLFPHQFINPRTNMTAHITVPYLGV


 


NRYDQYKVHKPWTLVVMVVAPLTVNNEGAPQIKVYANIAPTNVHVAGELP


 


SKEGIFPVACSDGYGGLVTTDPKTADPVYGKVFNPPRNLLPGRFTNLLDV


 


AEACPTFLHFDGDVPYVTTKTDSDRVLAQFDLSLAAKHMSNTFLAGLAQY


 


YTQYSGTINLHFMFTGPTDAKARYMVAYAPPGMEPPKTPEAAAHCIHAEW


 


DTGLNSKFTFSIPYLSAADYAYTASDVAETTNVQGWVCLFQITHGKADGD


 


ALVVLASAGKDFDLRLPVDARTQTTSAGESADPVTATVENYGGETQVQRR


 


QHTDIAFILDRFVKVKPKEQVNVLDLMQIPAHTLVGALLRTATYYFSDLE


 


LAVKHEGDLTWVPNGAPETALDNTTNPTAYHKEPLTRLALPYTAPHRVLA


 


TVYNGSSKYGDTSTNNVRGDLQVLAQKAERTLPTSFNFGAIKATRVTELL


 


YRMKRAETYCPRPLLAIQPSDARHKQRIVAPAKQLL






In one example, the polypeptide of this invention, upon binding to a receptor on a cell, e.g., such as integrin, induces death of the cell. Exemplary cells include an MCF-7 cell, a T-47D cell, a PC-3 cell, a 22Rv1 cell, a BHK-21 cell, and a HeLa cell. In another example, the polypeptide, upon binding to the receptor, represses the Akt signaling transduction pathway. In yet another example, the polypeptide, upon binding to the receptor, activates procaspase-9, -7, or -3, which further induces apoptosis.
As the polypeptide of this invention induces apoptosis, one therefore can use it to induce death of a cell by contacting a cell with the polypeptide. Thus, also within the scope of this invention are (i) a pharmaceutical composition that contains the above-described polypeptide and a pharmaceutically acceptable carrier, and (ii) a method for treating an apoptosis-related disorder in a subject, i.e., administering to the subject an effective amount of the just-mentioned polypeptide. “An apoptosis-related disorder” refers to a condition characterized or caused by an excessive level of cells. An excessive level refers to (1) a level higher than a normal level, and (2) a level higher than desired in an individual, even though it is not greater than a normal level. Examples of the disorder include a cancer (e.g., breast cancer, colorectal cancer, leukemia, liver cancer, lung cancer, ovarian cancer, or prostate cancer), an infection by a virus (e.g., that by human papillomavirus, human immunodeficiency virus, or Hepatitis virus), an allergic disease, an inflammatory disease, an autoimmune disease, an immunodeficiency disease, a reperfusion injury, or a neurodegenerative disorder.
This invention also features an isolated nucleic acid containing a sequence encoding the above-described polypeptide. Examples of the nucleic acid include a sequence encoding SEQ ID NO: 1 (e.g., SEQ ID NO: 2 listed above) and a sequence encoding SEQ ID NO: 7 (e.g., SEQ ID NO: 8 listed below)









 532
                                                        cgggacgtc



 


 541
cgcgcacgaa acgcgccgtc gcttgaggaa cacttgtaca aacacgattt aagcaggttt


 


 601
ccacaactga taaaactcgt gcaacttgaa actccgcctg gtctttccag gtctagaggg


 


 661
gttacacttt gtactgtgct cgactccacg cccggtccac tggcgggtgt tagtagcagc


 


 721
actgttgttt cgtagcggag catggtggcc gtgggaactc ctccttggtg acaagggccc


 


 781
acggggccga aagccacgtc cagacggacc caccatgtgt gcaaccccag cacggcaact


 


 841
tttactgcga acaccacctt aaggtgacac tggtactggt actcggtcac tggtgacagg


 


 901
ctaaggatgc ccttcaggta ccccgaggta acacgggaca ctcgggatct gagaagggga


 


 961
ttgggacttc tttaaaagtg cccagtttaa aaagcttcta cgcctgaata ggcgaccgga


 


1021
ggccggcgcc tttccattac ccactactaa atccatgaat acgactgact gttttatcgc


 


1081
tctgctatac gctctcagag agatcaaagc actgtttctg tcacgaacac aagggaagat


 


1141
ggaattcaca ctttacaacg gtgaaaagaa ggtcttctac tccagaccca acaaccacga


 


1201
caactgttgg ctgaacgcca tcctccaact gttcaggtac gttgacgagc ccttcctcga


 


1261
atgggtctac gactcacctg agaacctcac tctcgaggcg atcaacaaac tggaagaaat


 


1321
cacaggtctt gagctacacg agggcggacc gcccgccctt gtcgtctgga acatcaagca


 


1381
cttgctctac accggaatcg gcaccgcttc gcgacccagc gaggtgtgca tggtggacgg


 


1441
tacagacatg tgcttggctg acttccacgc cggtatattt ctgaagggac aggaccacgc


 


1501
cgtcttcgcc tgcgtcacct ctgacgggtg gtacgcgatt gacgacgagg acttttaccc


 


1561
gtggacacca aatccggccg acgttttggt ttttgttccg tacgatcaag aaccattcaa


 


1621
cgcagaatgg aaagcaaagg ttcagaagcg gctcaggggc gccgggcaat ccagcccgac


 


1681
gaccgggtca caaaaccaat ctggcaacac tggcagcatt attaacaatt actacatgca


 


1741
gcagtaccag aactcaatgg acacccaact tggcgacaac gccattagtg gagggtccaa


 


1801
cgagggctcc acggacacta cctctaccca caccaacaac acccagaaca acgactggtt


 


1861
ttcgaaactg gccaacaccg cttttagcgg cctcttcggt gctcttcttg cagacaagaa


 


1921
gacggaagaa accaccctcc tcgaagaccg catcctcacc acccgcaacg ggcacacgac


 


1981
ctcgacaacc cagtctagcg tcggggtgac ttacgggtac gcaacggctg aagacttcgt


 


2041
gagtgggcct aacacctctg gtcttgagac cagagttgtt caggccgaac ggttcttcaa


 


2101
aacccacctg tttgactggg tcaccagtga cccgtttggg cggtgtcact tgttggagct


 


2161
accgactgac cacaaaggcg tctacggtag cctgaccgac tcgtacgcat acatgaggaa


 


2221
tggttgggac gttgaagtca ccgcagtggg taaccaattc aacggaggct gtttgctggt


 


2281
ggcgatggta ccggagctct gttccatcag caagagagag ttgtaccagc ttacgctttt


 


2341
cccccaccag ttcatcaacc cacggacgaa tatgacggca cacatcaccg tgccctacct


 


2401
cggtgtcaac aggtacgacc agtacaaggt acacaaaccc tggaccctcg tggtcatggt


 


2461
tgtggccccc ttgacggtta acaacgaggg cgctccgcaa atcaaggtgt atgccaacat


 


2521
cgcccccacc aatgttcacg tcgcgggtga gctcccctct aaagagggga ttttccccgt


 


2581
ggcatgcagc gatggttacg gtggcttggt gaccacggat ccgaagacgg cagaccccgt


 


2641
ctacgggaaa gtgttcaacc caccccgcaa cctgttgcca gggcggttta caaacctcct


 


2701
tgacgtggcc gaggcgtgcc ccacattcct acacttcgac ggtgacgttc cgtacgtgac


 


2761
cacgaagacg gattcggata gggtgctagc ccagttcgat ttgtccctcg c






If the nucleic acid is operably linked to a regulatory sequence suitable for expressing the polypeptide in host cells, it can express the polypeptide after being introduced into the host cells. As the polypeptides thus-expressed, upon binding to a cell-surface receptor, can kill cells, including host cells, the nucleic acid can also be used for inducing cell death or treating an apoptosis-related disorder in a subject.
An “isolated polypeptide” refers to a polypeptide that has been substantially separated from other proteins, lipids, and nucleic acids with which it is naturally associated. The polypeptide can constitute at least 50, 70, or 95% by dry weight of the purified preparation. An “isolated nucleic acid” refers to a nucleic acid the structure of which is not identical to that of any naturally occurring nucleic acid or to that of any fragment of a naturally occurring genomic nucleic acid. The term therefore covers, for example, (a) a DNA which has the sequence of part of a naturally occurring genomic DNA molecule but is not flanked by both of the coding sequences that flank that part of the molecule in the genome of the organism in which it naturally occurs; (b) a nucleic acid incorporated into a vector or into the genomic DNA of a prokaryote or eukaryote in a manner such that the resulting molecule is not identical to any naturally occurring vector or genomic DNA; (c) a separate molecule such as a cDNA, a genomic fragment, a fragment produced by polymerase chain reaction (PCR), or a restriction fragment; and (d) a recombinant nucleotide sequence that is part of a hybrid gene, i.e., a gene encoding a fusion protein. The nucleic acid of this invention can be used to express a polypeptide of this invention. For this purpose, one can operatively link the nucleic acid to suitable regulatory sequences to generate an expression vector.
To produce a polypeptide of this invention, one can place a host cell in a culture under conditions permitting expression of a polypeptide encoded by a nucleic acid described above, and isolate the polypeptide from the culture. Alternatively, a nucleic acid of this invention can be transcribed and translated in vitro, for example, using T7 promoter regulatory sequences and T7 polymerase.
A vector refers to a nucleic acid molecule capable of transporting another nucleic acid to which it has been linked, and also capable of autonomous replication or integration into a host DNA. Examples include a plasmid, cosmid, and viral vector. A vector of this invention includes a nucleic acid in a form suitable for expression of the nucleic acid in a host cell. Preferably, the vector includes one or more regulatory sequences operatively linked to the nucleic acid sequence to be expressed. Examples of a regulatory sequence include promoters, enhancers, and other expression control elements (e.g., polyadenylation signals). Regulatory sequences also include those that direct constitutive expression of a nucleotide sequence, as well as tissue-specific regulatory and/or inducible sequences. The design of such an expression vector is based on considerations including the choice of the host cell to be transformed and the desired expression level. An expression vector can be introduced into host cells to produce a polypeptide of this invention. This invention also includes a host cell that contains the above-described nucleic acid. The host cell can be a bacterial cell, a yeast cell, an insect cell, a plant cell, and a mammalian cell.
The details of one or more embodiments of the invention are set forth in the accompanying drawings and the description below. Other features, objects, and advantages of the invention will be apparent from the description and drawings, and from the claims.


DETAILED DESCRIPTION
This invention is based, at least in part, on an unexpected discovery that a water-soluble foot-and-mouth disease virus (FMDV) VP1 polypeptide possesses apoptosis-inducing activity. The polypeptide and its variants are useful for treating conditions associated with disorders caused by excessive or unwanted cells.
Foot-and-mouth disease (FMD), a deadly epidemic, affects various economically important domestic livestock including cattle, pigs, goats, and sheep (Woolhouse et al., 2001, Nature 411, 258-259). FMDV includes seven serotypes of viruses, all of which belong to the Aphthovirus genus of the family picornaviridae. The capsid of FMDV is made up of 60 copies of each of four proteins, VP1, VP2, VP3, and VP4.
FMDV infects cells by attaching to cell-surface integrin through a long, conformationally flexible loop (G-H loop) of VP1 (Logan et al., 1993, Nature 362, 566-568). In some cases, it also uses heparin sulfate as an alternative internalization receptor. The G-H loop contains a conserved arginine-glycine-aspartic acid (RGD) tripeptide motif that is characteristic of integrin ligands (See, e.g., Ruoslahti et al., 2003, Matrix Biol. 22, 459-465).
Integrin belongs to a family of cell surface α-β heterodimeric glycoproteins. These proteins are responsible for a variety of processes, including the induction of signal transduction pathways that modulate cell proliferation, morphology, migration and apoptosis (Hynes, 1992, Cell 69, 11-25). Four species of integrin, i.e., αvβ1, αvβ3, αvβ6, and α5β1, have been shown to mediate FMDV infection (Jackson, et al., 2000, J. Virol. 74, 4949-4956 and Jackson, et al., 2002, J. Virol. 76, 935-941). Although the VP1-integrin interaction mediates FMDV infection, the study on VP1's biological effects is limited due to the poor water solubility of VP1. Indeed, VP1 protein has been only used together with denaturing agents such as urea, the presence of which has made it infeasible to evaluate the biological effects of VP1. Thus, there is a need for a water-soluble FMDV VP1 polypeptide.
This invention features a water-soluble FMDV VP1 polypeptide, as well as a nucleic acid encoding it. As mentioned above and described in the Example below, the water-soluble FMDV VP1 polypeptide, via binding to integrin, induces apoptosis in certain cancer cells. It is known that the binding of a ligand to integrin activates the Akt signal transduction pathway and protects cell from apoptosis (King, et al., 1997, Mol. Cell Biol. 17, 4406-4418 and Toker et al., 2000, Mol. Pharmacol. 57, 652-658). Thus, it is unexpected that the polypeptide of this invention binds to integrin and induces apoptosis. The polypeptide is useful for treating conditions associated with disorders caused by excessive or unwanted cells.
A polypeptide of the invention can be obtained as a synthetic polypeptide or a recombinant polypeptide. To prepare a recombinant polypeptide, one can clone a nucleic acid encoding the polypeptide in an expression vector, in which the nucleic acid is operably linked to a regulatory sequence suitable for expressing the polypeptide in a host cell. One can then introduce the vector into a suitable host cell to express the polypeptide. Alternatively, the nucleic acid can be linked to another nucleic acid encoding a fusion partner, e.g., Glutathione-S-Transferase (GST), T7 tag, 6×-His epitope tag, M13 Gene 3 protein, or an immunoglobulin heavy chain constant region. The resultant fusion nucleic acid expresses in suitable host cells a fusion protein. Suitable host cells are those that are resistant to this apoptotic polypeptide and can be obtained using screening methods known in the art. The expressed recombinant polypeptides can be purified from the host cell by methods such as ammonium sulfate precipitation and fractionation column chromatography. See Goeddel, (1990) Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, Calif. Water-soluble polypeptides are then prepared by the method described in U.S. application Ser. No. 10/449,531 and Wang et al., 2003, Vaccine 21, 3721-3729t. An isolated fusion protein can be further treated, e.g., by enzymatic digestion, to remove the fusion partner and obtain the recombinant polypeptide of this invention.
The amino acid composition of a polypeptide of the invention may vary without disrupting the ability of binding to integrin and inducing apoptosis. For example, such a variant can contain one or more conservative amino acid substitutions. A “conservative amino acid substitution” is one in which the amino acid residue is replaced with an amino acid residue having a similar side chain. Families of amino acid residues having similar side chains have been defined in the art. These families include amino acids with basic side chains (e.g., lysine, arginine, histidine), acidic side chains (e.g., aspartic acid, glutamic acid), uncharged polar side chains (e.g., glycine, asparagine, glutamine, serine, threonine, tyrosine, cysteine), nonpolar side chains (e.g., alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, tryptophan), beta-branched side chains (e.g., threonine, valine, isoleucine) and aromatic side chains (e.g., tyrosine, phenylalanine, tryptophan, histidine). Thus, a predicted nonessential amino acid residue in a polypeptide is preferably replaced with another amino acid residue from the same side chain family. Alternatively, mutations can be introduced randomly along all or part of a polypeptide of this invention, such as by saturation mutagenesis, and the resultant mutants can be screened for the ability of binding to integrin and inducing apoptosis to identify variants of this invention as descried below in the example. Thus, as an example, the term “polypeptide containing SEQ ID NO: 1” covers polypeptides containing variants of SEQ ID NO: 1, including fusion proteins or proteins having one or more conservative amino acid substitutions mutations, insertions, deletions, truncations, or combination thereof. Each variant retains substantially the activity of binding to integrin and inducing apoptosis.
Each of the above-described polypeptides can be tested for its apoptotic activity on cells according to the method described in the Example below. A polypeptide having apoptotic activity, as well as nucleic acid encoding it, can be used to induce cell death.
Thus, also within the scope of this invention is a method of inducing death of cells, e.g., by contacting cells with a polypeptide of the invention in vitro, or by administering to a subject in need thereof an effective amount of the polypeptide or nucleic acid, e.g., an expression vector. Subjects to be treated can be identified as having or being at risk for acquiring an apoptosis-related disorder.
The term “treating” refers to administration of a composition to a subject with the purpose to alleviate, relieve, remedy, or ameliorate a disorder, the symptom of the disorder, the disease state secondary to the disorder, or the predisposition toward the disorder. An “effective amount” is an amount of the composition that is capable of producing a medically desirable result in a treated subject. The method can be performed alone or in conjunction with other drugs or therapy.
Disorders to be treated include a disease caused by excessive abnormal cells (e.g., cancerous cells) or excessive normal cells (e.g., T-cells). Examples of a disease caused by excessive abnormal cells, i.e., oncological disease, include retinoblastoma, Wilm's tumor, familial colonic polyposis, hereditary non polyposis colon cancer, neurofibromatosis, familial chest cancer, xeroderma pigmentosum, blain cancer, oral cancer, esophageal cancer, stomach cancer, colon cancer, liver cancer, pancreatic cancer, lung cancer, thyroid cancer, mammary gland tumor, urinary tumor, virilia tumor, muliebria tumor, skin tumor, osteosarcoma, osteochondrosarcoma, leukemia, lymphoma, and solid tumor. Exemplary diseases caused by excessive T-cells include diabetes mellitus, arthritis (including rheumatoid arthritis, juvenile rheumatoid arthritis, osteoarthritis, and psoriatic arthritis), multiple sclerosis, encephalomyelitis, myasthenia gravis, systemic lupus erythematosis, autoimmune thyroiditis, dermatitis (including atopic dermatitis and eczematous dermatitis), psoriasis, Sjögren's Syndrome, Crohn's disease, aphthous ulcer, iritis, conjunctivitis, keratoconjunctivitis, type I diabetes, inflammatory bowel diseases, ulcerative colitis, asthma, allergic asthma, cutaneous lupus erythematosus, scleroderma, vaginitis, proctitis, drug eruptions, leprosy reversal reactions, erythema nodosum leprosum, autoimmune uveitis, allergic encephalomyelitis, acute necrotizing hemorrhagic encephalopathy, idiopathic bilateral progressive sensorineural hearing loss, aplastic anemia, pure red cell anemia, idiopathic thrombocytopenia, polychondritis, Wegener's granulomatosis, chronic active hepatitis, Stevens-Johnson syndrome, idiopathic sprue, lichen planus, Graves' disease, sarcoidosis, primary biliary cirrhosis, uveitis posterior, interstitial lung fibrosis, graft-versus-host disease, cases of transplantation (including transplantation using allogeneic or xenogeneic tissues) such as bone marrow transplantation, liver transplantation, or the transplantation of any organ or tissue, allergies such as atopic allergy, and AIDS.
In one in vivo approach, a therapeutic composition (e.g., a composition containing a polypeptide of the invention or a nucleic acid encoding it) is administered to a subject. Generally, the polypeptide or nucleic acid is suspended in a pharmaceutically-acceptable carrier (e.g., physiological saline) and administered orally or by intravenous infusion, or injected or implanted subcutaneously, intramuscularly, intrathecally, intraperitoneally, intrarectally, intravaginally, intranasally, intragastrically, intratracheally, or intrapulmonarily.
The dosage required depends on the choice of the route of administration; the nature of the formulation; the nature of the subject's illness; the subject's size, weight, surface area, age, and sex; other drugs being administered; and the judgment of the attending physician. Suitable dosages are in the range of 0.01-100.0 mg/kg. Variations in the needed dosage are to be expected in view of the variety of compositions available and the different efficiencies of various routes of administration. For example, oral administration would be expected to require higher dosages than administration by intravenous injection. Variations in these dosage levels can be adjusted using standard empirical routines for optimization as is well understood in the art. Encapsulation of the composition in a suitable delivery vehicle (e.g., polymeric microparticles or implantable devices) may increase the efficiency of delivery, particularly for oral delivery.
Also within the scope of this invention is a pharmaceutical composition that contains a pharmaceutically acceptable carrier and an effective amount of a polypeptide of the invention or a nucleic acid encoding it. The pharmaceutical composition can be used to treat diseases described above. The pharmaceutically acceptable carrier includes a solvent, a dispersion medium, a coating, an antibacterial and antifungal agent, and an isotonic and absorption delaying agent.
The pharmaceutical composition of the invention can be formulated into dosage forms for different administration routes utilizing conventional methods. For example, it can be formulated in a capsule, a gel seal, or a tablet for oral administration. Capsules can contain any standard pharmaceutically acceptable materials such as gelatin or cellulose. Tablets can be formulated in accordance with conventional procedures by compressing mixtures of the composition with a solid carrier and a lubricant. Examples of solid carriers include starch and sugar bentonite. The composition can also be administered in a form of a hard shell tablet or a capsule containing a binder, e.g., lactose or mannitol, a conventional filler, and a tableting agent. The pharmaceutical composition can be administered via the parenteral route. Examples of parenteral dosage forms include aqueous solutions, isotonic saline or 5% glucose of the active agent, or other well-known pharmaceutically acceptable excipient. Cyclodextrins, or other solubilizing agents well known to those familiar with the art, can be utilized as pharmaceutical excipients for delivery of the therapeutic agent.
The efficacy of a composition of this invention can be evaluated both in vitro and in vivo. See, e.g., the examples below. Briefly, the composition can be tested for its ability to induce death of cells in vitro. For in vivo studies, the composition can be injected into an animal (e.g., a mouse model) and its therapeutic effects are then accessed. Based on the results, an appropriate dosage range and administration route can be determined.
The specific example below is to be construed as merely illustrative, and not limitative of the remainder of the disclosure in any way whatsoever. Without further elaboration, it is believed that one skilled in the art can, based on the description herein, utilize the present invention to its fullest extent. All publications recited herein are hereby incorporated by reference in their entirety.
Example
VP1 Induced Apoptosis
To examine whether VP1 induces apoptosis, aqueous soluble recombinant VP1 (rVP1) was expressed in E. coli and purified according to the method described in U.S. application Ser. No. 10/449,531 and Wang et al., 2003, Vaccine 21, 3721-3729. Since aqueous soluble rVP1 might form monomers or dimers depending upon redox conditions during experiments, a mutant rVP1 monomer was generated by changing the position 201 cysteine to serine via site-directed mutagenesis following the protocol described in Du et al., 1995, Biotechniques 18, 376-378. The mutated VP1 gene was ligate into the expression vector pET24a(+). The mutant and wild type proteins were expressed in E. coli and purified in the same manner described in the aforementioned two references.
Baby hamster kidney fibroblast cells (BHK-21) were used to examine the apoptosis-inducing activity of wild type and mutant rVP1. BHK-21 cells are known to be permissive for FMDV to bind to through the RGD motif and replicate (Baxt et al., 1984, J. Virol. 51, 298-305). More specifically, BHK-21 cells were maintained at 37° C. in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal calf serum (FCS), 2 mM L-glutamine, 100 U/ml penicillin, and 100 μg/ml streptomycin. The cells were plated at 2×105 cells per well in a twelve-well plate (400 μl/well). One day later, the cells were washed twice with phosphate-buffered saline (PBS) and incubated with a FCS-free DMEM containing 1 μM rVP1. The cells were then incubated at 37° C. overnight before being examined for DNA fragmentation, a hallmark of apoptosis (Slee, et al., 1999. J. Cell Biol. 144, 281-292).
Briefly, the BHK-21 cells treated with rVP1 were washed by ice-cold PBS and transferred to centrifuge tubes. The cells in each tube were recovered by centrifugation at 1,500×g for 10 minutes at 4° C. and re-suspended in 100 μl of a solution containing 10 mM Tris-Cl and 1 mM EDTA, pH 8.0. The suspension was mixed with 1 ml of an extraction buffer containing 0.1 M EDTA, 0.5% (w/v) SDS, 20 μg/ml pancreatic RNase and 10 mM Tris-Cl, pH 8.0 and incubated at 37° C. for 1 hour. Proteinase K (Sigma) was then added to a final concentration of 100 μg/ml and the suspension of lysed cells was incubated at 50° C. for 3 hours with periodically swirling. An equal volume of phenol was then added to the suspension. The mixture was kept at room temp for 10 minutes and then centrifuged at 5,000×g for 15 minutes to separate into two phases. After three times extraction with phenol, the aqueous phases were pooled, transferred to a centrifuge tube, and mixed with 0.2 volume of 10 M ammonium acetate as well as 2 volumes of ethanol. The genomic DNA was collected by centrifugation at 5,000×g for 5 minutes, washed with 70% ethanol, separated by electrophoresis on a 0.8% agarose gel, and examined for DNA fragmentation.
It was found that genomic DNA prepared from BHK-21 cells treated with wild type or mutant rVP1 was fragmented to about the same degree. This result indicates that both the with wild and mutant rVP1 proteins can induce apoptosis in BHK-21 cells. The mutated monomeric rVP1 was used in all following experiments.
BHK-21 cells were further used to evaluate whether the above-mentioned DNA fragmentation was accompanied by cell death and DNA condensation. Briefly, BHK-21 cells (5×105 cells ml−1) were transfected with a plasmid encoding green fluorescent protein gene (pEGFP-C3, 0.5 μg/well, BD Biosciences, Palo Alto, Calif.) in Effectene (Qiagen, Valencia, Calif.) for 24-48 hours. The cells were then incubated with a medium containing 0 (control) or 1.0 μM rVP1 for 2 or 24 hours. The living cells, which emitted green fluorescent upon excitation, were identified and counted under a fluorescence microscope before and after the rVP1 incubation. It was found that the number of living cells was reduced after 2 hours of incubation and further reduced after 24 hours of incubation.
To visualize DNA condensations in nuclei, BHL-21 cells were treated with 0 (control), 0.05, 0.1, 0.5, 1.0, or 3.0 μM rVP1 for 16 hours. The cells were then stained with DAPI (0.5 μg/ml in PBS, Sigma, St. Louis, Mo.) according to the manufacturer's instructions and examined by light microscopy. The level of apoptosis was determined as the ratio of the cells with morphologically changed nuclei to the total number of cells present, i.e., apoptotic index. It was found that apoptotic indexes of the cells treated with 0.1, 0.5, 1.0, and 3.0 μM rVP1 were about 10%, 90%, 95%, and 99%, respectively. In contrast, the apoptotic index of the control cells was less than 1%. These results indicate that rVP1 indeed causes apoptosis.
To compare the apoptotic effect of rVP1 with those of other RGD containing molecules, BHK-21 cells were treated with rVP1, cyclic RGD, or P29 peptide (peptide containing sequence of amino acid residues of 139-164 with RGD motif at position 145-147) in the manner descried above. It was found that the treatment by 1 μM rVP1 induced apoptosis in almost all BHK-21 cells, while cyclic RGD at the same concentration had little effect. The 50% effective concentration of rVP1 in causing apoptosis was around 0.3 μM which was about 100 fold and 10,000 fold more potent than that of the P29 peptide (30 μM) and cyclic RGD (3 mM), respectively.
VP1 Induced Apoptosis via Binding to Integrin
The above-described experiments were repeated except that, before incubating with rVP1, the cells were incubated with rabbit anti-FMDV neutralizing antibodies (RN) or mouse anti-integrin VLA-5 (α5β1) monoclonal antibody (Chemicon, Temecula, Calif.). These antibodies are known to block the binding of integrin to its ligand. It was found that the apoptotic effect of rVP1 was blocked specifically by these two antibodies. It is known that fibronectin is a natural ligand for integrin α5β1. The effects of fibronectin on rVP1-induced apoptosis in BHK-21 cells were investigated in the manner described above. The result showed that fibronectin at 0.8 μM abolished the apoptotic effect of 1 μM rVP1. Both of the above results indicate that rVP1 induces apoptosis via binding to integrin.
To further demonstrate that the apoptotic effect of rVP1 requires the interaction between rVP1 and integrin, rVP1 was expressed in BHK-21 cells intracellularly. More specifically, BHK-21 cells were transfected with 0.3 μg plasmid pIBSY1 or pIBSY1-VP1, an expression vector encoding VP1 (Shieh, et al., 2001, Vaccine 19, 4002-4010). The transfected cells were subjected to apoptosis assays in the same manner described above. It was found that, although rVP1 was significantly expressed in the cells transfected with pIBSY1-VP1, little apoptosis was observed even after 3-days of culture. On the other hand, addition of rVP1 (0.5 μM) to the culture medium resulted in the death of the transfected cells. These results suggest that induction of apoptosis by rVP1 is due to its binding to extracellular domains of integrin.
It is known that integrin signaling activates the PI3-K/Akt signal transduction pathway, an anti-apoptotic mechanism utilized by many types of cells (King et al., 1997, Mol. Cell Biol. 17, 4406-4418 and Toker 2000, Mol Pharmacol 57, 652-658. Akt, a serine/threonine kinase, can bind to phosphorylated lipids at the membrane in response to the activation of phosphatidylinositol 3-kinase (PI3-kinase) by growth factors and mediates cell survival. Phosphorylated Akt activates anti-apoptotic or inhibits pro-apoptotic processes in a cell by phosphorylating Bad, Forkhead transcription factors, glycogen synthase kinase 3β (GSK-3β), and caspase-9 (See, e.g., Benetti et al., 2003, J. Virol. 77, 6567-6573). Deactivation of Akt, on the other hand, activates pro-apoptotic responses (Luo, et al., 2003, Proc. Natl. Acad. Sci. USA 100, 11712-11717). Both GSK-3β and caspase-9 are pro-apoptotic factors. Cleavage of caspase-9, a cysteine aspartic acid protease, results in induction of a caspase cascade, including the processing of procaspase-3 and -7, which eventually leads to apoptosis. Activation of PI3-kinase through G protein-coupled receptors such as sphingosine-1-phosphate (S1P) receptors or PI3-kinase inhibitors (e.g., LY294002) modulates the activity of Akt. Thus, PI3-K/Akt signal transduction pathway, GSK-3β, and caspases were further analyzed.
VP1 Treatment Deactivated Akt
In this experiment, the PI3-K/Akt signal transduction pathway was examined in BHK-21 cells treated with rVP1. As binding of PDGF to cells activates the pathway and causes cell proliferation (Slee, et al., 1999, J. Cell Biol. 144, 281-29228, and Schneller et al., 1997, Embo. J. 16, 5600-5607), BHK-21 cells were incubated with PDGF (PeproTech EC LTD, London, United Kingdom) and rVP1, respectively.
More specifically, BHK-21 cells were incubated with rVP1 in the manner described above in presence or absence of PDGF (0.1 μg). At minutes 5, 30, and 60, the cells were lysed in 0.2 ml of a boiling protein loading buffer (Invitrogen). Twenty microliter of each boiled sample was analyzed for Akt and GSK-3β phosphorylation by Western blotting using primary antibodies against Akt, phospho-Akt (Ser473), and phospho-GSK-3β (Ser9), as well as anti-actin, anti-integrin VLA-5 (α5β1) monoclonal antibody, and anti-mouse immunoglobulin G horseradish peroxidase-coupled secondary antibodies. The antibodies against Akt, phospho-Akt (Ser473), and phospho-GSK-3β (Ser9) were obtained from Cell Signaling Technology, Inc (Beverly, Mass.). Anti-actin, anti-integrin VLA-5 (α5β1) antibody, and anti-mouse immunoglobulin G horseradish peroxidase-coupled secondary antibodies were obtained from Chemicon (Temecula, Calif.).
As expected, PDGF activated Akt phosphorylation, and this effect was inhibited by 10 μM PI-3K inhibitor LY294002 (Cell Signaling Technology, Inc, Beverly, Mass.). It was found that rVP1 incubation inhibited Akt phosphorylation in a dose dependent manner after BHK-21 cells were incubated with rVP1 for 30 minutes. This inhibitory effect was reversed by pre-treatment of cells with anti-integrin α5β1 antibodies for 30 min, indicating that integrin was required for the inhibition.
Phosphorylation of GSK-3β was also examined according to the method described in Cross et al., 1995, Nature 378, 785-789. It was found that rVP1 inhibited the phosphorylation of GSK-3β in a dose dependent manner.
To verify that the level of phosphorylated GSK-3β correlated positively with BHK-21 cell survival, BHK-21 cells were treated with GSK-3β inhibitor (GSK-3βI, Calbiochem) thereby inhibiting the formation of GSK-3β according to the method described in Coghlan et al., 2000, Chem. Biol. 7, 793-803. The results showed that treatment of BHK-21 cells with GSK-3βI attenuated the apoptotic effect of rVP1 in a dose-dependent manner, indicating that rVP1 induces apoptosis via GSK-3β.
VP1 Activated Procaspase Cleavage
Caspases, a family of cysteine aspartic acid proteases, are central regulators of apoptosis (Slee et al., 1999, J. Cell Biol. 144, 281-292). Fragmentation of DNA causes cleavage of procaspase-9, which in turn results in processes of other procaspases, such as procaspase-3 and procaspase-7. The initiation of the caspase cascade eventually leads to apoptosis. Thus, the effects of rVP1 treatment on the activation of procaspase-9 and downstream procaspases-7 and -3 were evaluated.
Procaspases-3, -7, and -9 were obtained from Cell Signaling Technology, Inc (Beverly, Mass.). BHK-21 cells were treated with increasing amounts of rVP1 (0, 0.1, 0.2, and 0.5 μM) in the same manner described above. After incubation for 16 hours, the cells were lysed. The resultant lysates were analyzed by Western blot using antibodies against procaspases-3, -7, and -9.
It was found that the rVP1 treatment caused the cleavage of procaspases-9, -7 and -3. Cleavage of both procaspases-9 and -7 was detected in BHK-21 cells treated with 0.1 μM of rVP1 and became more pronounced at 0.5 μM rVP1. Cleavage of procaspase-3 was not detectable until higher concentrations (≧0.5 μM) of rVP1 were used. These results indicate that rVP1 induces cleavage of procaspases.
VP1 Induced Apoptosis in Cancer Cells
The aforementioned Akt signal transduction pathway is a major target for treatment of all four major human cancers, i.e., breast, prostate, lung, and colorectal cancers (See, e.g., Roy et al., 2002, Carcinogenesis 23, 201-205). In the following experiments, the effects of rVP1 on Akt signal transduction pathway in cancer cells were examined.
First, the effects of rVP1 on the Akt signal transduction pathway was evaluated in breast carcinoma MCF-7 cells. More specifically, MCF-7 cells were incubated with rVP1 in presence or absence of GSK-3β inhibitor I (Calbiochem) in the same manner described above. It was found that (1) rVP1 caused apoptosis in MCF-7 cells in a dose-dependent manner (2) this rVP1-induced apoptosis was reversed by GSK-3β inhibitor I in a dose-dependent manner. As MCF-7 cells lack a functional caspase-3 gene, these results suggest that the apoptotic effect of rVP1 is independent of caspase-3 and differ from that of Buckley et al., 1999, Nature 397, 534-539, which showed that seven RGD-containing polypeptide were unable to induce apoptosis in MCF-7 cells. These results also suggest that the VP1 polypeptide and its functional equivalents are different form the Buckley polypeptides and can be used to treat breast cancer. Indeed, none of the Buckley polypeptides contains RGDL.
Second, prostate cancer cell lines PC-3 and 22Rv1 were used to examine the apoptotic effects of rVP1. As mentioned above, activation of PI3-kinase through S1P receptors modulates Akt activity. In fact, binding of S1P to S1P receptors subtype 3 (S1P3) mediates Akt activation and crosstalk with PDGF receptor. It is known that 22Rv1 cells express S1P3 and are responsive to androgen stimulation. In contrast, PC-3 cells do not express S1P3 and are non-responsive to androgen (Baudhuin, et al., 2004, Faseb. J. 18, 341-343). The two types of cells were maintained at 37° C. in DMEM-10% FCS containing 2 mM L-glutamine, 100 U/ml penicillin, and 100 mg/ml streptomycin. The cells were plated at 2×105 cells per well in a twelve-well plate (400 μl/well). One day later, the cells were incubated with media containing 0, 0.2, 0.4, 0.8, and 1.2 μM of rVP1, respectively, and subjected to apoptosis assays in the manner described the experiments. The results showed that rVP1 caused apoptosis in both cell types and exerted more apoptotic effect on PC-3 cells. These results suggest that the apoptotic effect of rVP1 is not via deactivation of S1P3. Further, they suggest that rVP1 can be used to treat androgen-independent prostate cancers, such as PC-3, which are usually much more difficult to cure than androgen-dependent prostate cancers.
Other Embodiments
All of the features disclosed in this specification may be combined in any combination. Each feature disclosed in this specification may be replaced by an alternative feature serving the same, equivalent, or similar purpose. Thus, unless expressly stated otherwise, each feature disclosed is only an example of a generic series of equivalent or similar features.
From the above description, one skilled in the art can easily ascertain the essential characteristics of the present invention, and without departing from the spirit and scope thereof, can make various changes and modifications of the invention to adapt it to various usages and conditions. Thus, other embodiments are also within the scope of the invention.

## Claims

1. A method for treating cancer in a subject, the method comprising administering to a subject in need thereof an effective amount of a water-soluble VP1 polypeptide of foot-and-mouth disease virus, wherein the VP1 polypeptide contains RGD, binds to integrin, and induces cell death.

2. The method of claim 1, wherein the VP1 polypeptide contains the amino acid sequence of SEQ ID NO:1.

3. The method of claim 1, wherein the VP1 polypeptide contains the amino acid sequence of SEQ ID NO:3.

4. The method of claim 1, wherein the cancer is selected from the group consisting of breast cancer, leukemia, liver cancer, lung cancer, ovarian cancer, and prostate cancer.

5. The method of claim 4, wherein the VP1 polypeptide contains the amino acid sequence of SEQ ID NO:1.

6. The method of claim 4, wherein the VP1 polypeptide contains the amino acid sequence of SEQ ID NO:3.

7. The method of claim 1, wherein the cancer is liver cancer or prostate cancer.

8. The method of claim 7, wherein the VP1 polypeptide contains the amino acid sequence of SEQ ID NO:1.

9. The method of claim 7, wherein the VP1 polypeptide contains the amino acid sequence of SEQ ID NO:3.

10. The method of claim 1, wherein the cancer is ovarian cancer or breast cancer.

11. The method of claim 10, wherein the VP1 polypeptide contains the amino acid sequence of SEQ ID NO:1.

12. The method of claim 10, wherein the VP1 polypeptide contains the amino acid sequence of SEQ ID NO:3.

13. A method for inducing apoptosis in a subject having cancer, the method comprising administering to a subject in need thereof an effective amount of a water-soluble VP1 polypeptide of foot-and-mouth disease virus, wherein the VP1 polypeptide contains RGD, binds to integrin, and induces cell death.

14. The method of claim 13, wherein the cancer is selected from the group consisting of breast cancer, leukemia, liver cancer, lung cancer, ovarian cancer, and prostate cancer.

15. The method of claim 14, wherein the VP1 polypeptide contains the amino acid sequence of SEQ ID NO:1.

16. The method of claim 14, wherein the VP1 polypeptide contains the amino acid sequence of SEQ ID NO:3.

17. The method of claim 13, wherein the cancer is liver cancer or prostate cancer.

18. The method of claim 17, wherein the VP1 polypeptide contains the amino acid sequence of SEQ ID NO:1 or 3.

19. The method of claim 13, wherein the cancer is ovarian cancer or breast cancer.

20. The method of claim 19, wherein the VP1 polypeptide contains the amino acid sequence of SEQ ID NO:1 or 3.