# Patent

## Title
Identification and isolation of human corneal endothelial cells (HCECs)

## Abstract

The present invention provides methods for the identification, isolation and/or enrichment of human corneal endothelial cells (HCECs). In some embodiments, the method comprises a positive selection process in which a cell population containing human corneal cells is contacted with a positive affinity reagent that selectively binds to HCECs relative to cells other than HCECs (e.g., corneal keratocytes, etc.) in the population and/or a negative selection process in which a cell population containing HCECs is contacted with a negative affinity reagent that selectively binds to cells other than HCECs in the population relative to HCECs. The present invention also provides reagents and kits for the identification, isolation and/or enrichment of HCECs as well as compositions that are enriched in HCECs.

## Background
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a continuations of U.S. patent application Ser. No. 14/888,875, filed Nov. 3, 2015 (published as US20160102290), which is the U.S. National Stage of International Patent Application No. PCT/US2014/036616, filed May 2, 2014, which claims the benefit of U.S. Patent Application No. 61/819,146, entitled “METHOD FOR IDENTIFYING AND ISOLATING HUMAN CORNEAL ENDOTHELIAL CELLS (HCECS),” filed May 3, 2013, the contents of each of which are hereby incorporated by reference in their entireties.


INCORPORATION-BY-REFERENCE OF MATERIAL ELECTRONICALLY FILED
Incorporated by reference in its entirety herein is a computer-readable nucleotide/amino acid sequence listing submitted concurrently herewith and identified as follows: One 821,867 byte ASCII (text) file named “Seq_List” created on Aug. 13, 2018.
BACKGROUND OF THE INVENTION
When the innermost layer of the cornea, the endothelium, is damaged, for example from trauma (e.g., from cataract surgery), disease or dystrophy, the cornea swells with fluid (edema) and loses its optical clarity. Patients consequently suffer from vision loss and pain, and their only option to treat advanced disease is with corneal transplant surgery (also known as penetrating keratoplasty, PK) or Descemet's stripping endothelial keratoplasty (DSAEK), both technically difficult procedures that are very invasive to the patient and have significant limitations, such as the number of donor corneas available.
Recent studies have proposed the use of human corneal endothelial cells (HCECs) obtained from cadaveric donors to replace the damaged cells. See, e.g., Joyce and Zhu, Cornea. 2004 November; 23(8 Suppl):S8-S19; Engelmann, et al., Exper. Eye Res., vol. 78, no. 3, pp. 573-578, 2004. A potential advantage to such an approach could be the expansion of HCECs ex vivo before implantation into patients, thereby overcoming the limited tissue availability. HCECs can be expanded in defined tissue culture media for at least 5 passages, greatly expanding the number of cells derived from a single donor.
One of the main problems with such a technique is that the lack of defined surface markers specific for HCECs makes it difficult to confirm the identity of HCECs after several passages, or to select HCECs away from contaminating cells, or to identify the subset of HCECs that are likely to have the highest clinical efficacy from among the full population of HCECs, as current identification criteria are limited to cell morphology and the expression of functional genes, such as ATP1A1 (see, e.g., Kaye and Tice, Invest Ophthalmol 1966; 522-32; Leuenberger and Novikoff, J Cell Biol. 1974; 60721-731; McCartney et al., Curr Eye Res, 1987; 61479-1486) or the tight junction marker zonula occludens-1(ZO-1) (see, e.g., Petroll et al., Curr Eye Res. 1999 January; 18(1):10-9), neither of which are specific to HCECs. It is also difficult to isolate HCECs from contaminant fibroblasts in culture, from neighboring cells in whole corneas, or from residual corneas from DSAEK.
In this regard, the current isolation method for obtaining HCECs from intact corneas comprises a peel-off step, where the endothelium and its basement membrane (Descemet's membrane) are peeled off the stroma and collected. See, e.g., Ko-Hua Chen et al., “Transplantation of Adult Human Corneal Endothelium Ex Vivo: A Morphologic Study,” Cornea 20(7): 731-737, 2001. The tissue collected thus contains HCECs, but it may also contain corneal keratocytes (specialized fibroblasts residing the stroma). Corneal keratocytes (also referred to herein simply as “keratocytes”) are undesirable contaminants in the HCECs culture, as they grow faster than the latter cells and they can take over the culture dish, thus making the final product essentially useless. In addition to residual stromal tissue, keratocytes may also arise from human endothelial cells which transform spontaneously into other types of cells such as keratocytes (see, e.g., G S. L. Peh et al., “Optimization of Human Corneal Endothelial Cells for Culture: The Removal of Corneal Stromal Fibroblast Contamination Using Magnetic Cell Separation,” International Journal of Biomaterials, Volume 2012 (2012), Article ID 601302, 8 pages.)
SUMMARY OF THE INVENTION
Some aspects of the invention are directed to methods for the identification, enrichment and/or isolation of human corneal endothelial cells (HCECs).
In some embodiments, the method comprises a positive selection process in which a cell population containing human corneal cells is contacted with a positive affinity reagent that selectively binds to HCECs relative to cells other than HCECs. The cells to which the positive affinity reagent is bound are then selected, with the result being that selected cells are enriched with HCECs. In some embodiments, two or more differing positive affinity reagents which bind to HCECs but which do not bind to cells other than HCECs are employed.
As defined herein “cells other than human corneal endothelial cells” (or “cells other than HCECs”) include corneal keratocytes as well as HCECs of lower utility (e.g., HCECs that have undergone fibroblastic or mesenchymal transformation, etc.).
In other embodiments, the method comprises a negative selection process in which a cell population containing human corneal cells is contacted with a negative affinity reagent that selectively binds to cells other than HCECs (e.g., corneal keratocytes, etc.) relative to HCECs. The cells to which the negative affinity reagent is bound are then removed, with the result being that the cells that are not removed are enriched with HCECs. In some embodiments, two or more differing negative affinity reagents which bind to cells other than HCECs (e.g., corneal keratocytes, etc.) but which do not bind to HCECs are employed.
In some embodiments, the method comprises both (a) positive selection using one or more affinity reagents agents and (b) negative selection using one or more negative affinity reagents.
Other aspects of the invention are directed to affinity reagents and kits useful for the identification, enrichment and/or isolation of HCECs.
Still other aspects of the invention are direct to isolated and/or enriched cell populations that contain HCECs. In some embodiments, such enriched cell populations may contain affinity reagents for the purification of HCECs.
Further aspects of the invention a set forth in the following paragraphs:
Aspect 1. A method of forming a composition enriched with human corneal endothelial cells comprising: (a) contacting a cell population containing human corneal cells with a first positive affinity reagent that selectively binds to human corneal endothelial cells relative to cells other than human corneal endothelial cells and (b) selecting cells to which the first positive affinity reagent is bound.
Aspect 2. The method of aspect 1, wherein the first positive affinity reagent selectively binds to human corneal endothelial cells relative to corneal keratocytes, human corneal endothelial cells of lower utility, or both.
Aspect 3. The method of aspect 1, wherein the first positive affinity reagent selectively binds to a corneal protein selected from protein products of genes X1 through X26 of Table 2, and may comprise, for example, an antibody or aptamer that binds to a corneal protein selected from protein products of genes X1 through X26 of Table 2, including an antibody or aptamer that binds to one or more proteins selected from SEQ ID NO (1) through SEQ ID NO (58) of Table 2.
Aspect 4. The method of aspect 1, wherein the first positive affinity reagent comprises an antibody or aptamer that binds to a protein product of gene X5 of Table 2, an antibody or aptamer that binds to a protein product of gene X15 of Table 2, or an antibody or aptamer that binds to a protein product of X25 of Table 2.
Aspect 5. The method of aspect 1, wherein the first positive affinity reagent comprises (a) an antibody or aptamer that binds to SEQ ID NO (8), (b) an antibody or aptamer that binds to one or more of SEQ ID NO (27), SEQ ID NO (28), SEQ ID NO (29), SEQ ID NO (30) or SEQ ID NO (31), or (c) an antibody or aptamer that binds to one or more of SEQ ID NO (53), SEQ ID NO (54), SEQ ID NO (55), SEQ ID NO (56) or SEQ ID NO (57).
Aspect 6. The method of any of aspects 1-5, wherein the first positive affinity reagent comprises an antibody or aptamer that is coupled to a solid matrix.
Aspect 7. The method of any of aspects 1-6, wherein the first positive affinity reagent comprises an antibody or aptamer that is coupled to a label.
Aspect 8. The method of aspect 7, wherein the label is selected from a magnetic label, a hapten (e.g., biotin) and a fluorescent label.
Aspect 9. The method of any of aspects 1-8, further comprising (a) contacting said cell population containing human corneal cells with a second positive affinity reagent that selectively binds to human corneal endothelial cells relative to cells other than human corneal endothelial cells and (b) selecting cells to which the second positive affinity reagent is bound, wherein the second positive affinity reagent differs from the first positive affinity reagent.
Aspect 10. The method of aspect 9, wherein the second positive affinity reagent selectively binds to a corneal protein selected from protein products of genes X1 through X26 of Table 2, and may comprise, for example, an antibody or aptamer that binds to a corneal protein selected from protein products of genes X1 through X26 of Table 2, including an antibody or aptamer that binds to one or more proteins selected from SEQ ID NO (1) through SEQ ID NO (58) of Table 2.
Aspect 11. The method of aspect 9, wherein the second positive affinity reagent comprises an antibody or aptamer that binds to a protein product of gene X5 of Table 2, an antibody or aptamer that binds to a protein product of gene X15 of Table 2, or an antibody or aptamer that binds to a protein product of X25 of Table 2.
Aspect 12. The method of aspect 9, wherein the second positive affinity reagent comprises (a) an antibody or aptamer that binds to SEQ ID NO (8), (b) an antibody or aptamer that binds to one or more of SEQ ID NO (27), SEQ ID NO (28), SEQ ID NO (29), SEQ ID NO (30) or SEQ ID NO (31), or (c) an antibody or aptamer that binds to one or more of SEQ ID NO (53), SEQ ID NO (54), SEQ ID NO (55), SEQ ID NO (56) or SEQ ID NO (57).
Aspect 13. The method of any of aspects 9-12, wherein the second positive affinity reagent comprises an antibody or aptamer that is coupled to a solid matrix or a label.
Aspect 14. The method of any of aspects 1-13, further comprising (a) contacting said cell population containing human corneal cells with a first negative affinity reagent that selectively binds to cells other than human corneal endothelial cells relative to human corneal endothelial cells and (b) removing the cells to which the first negative affinity reagent is bound.
Aspect 15. The method of aspect 14, wherein the first negative affinity reagent selectively binds to corneal keratocytes, human corneal endothelial cells of lower utility, or both, relative to human corneal endothelial cells.
Aspect 16. The method of aspect 14, wherein the first negative affinity reagent selectively binds to a corneal protein selected from protein products of genes Y1 through Y23 of Table 2, and may comprise, for example, an antibody or aptamer that binds to a corneal protein selected from protein products of genes Y1 through Y23 of Table 2, including an antibody or aptamer that binds to one or more proteins selected from SEQ ID NO (59) through SEQ ID NO (96) of Table 2, or wherein the first negative affinity reagent selectively binds to a corneal protein selected from protein products of genes Z1 through Z8 of Table 2, and may comprise, for example, an antibody or aptamer that binds to a corneal protein selected from protein products of genes Z1 through Z8 of Table 2, including an antibody or aptamer that binds to one or more proteins selected from SEQ ID NO (97) through SEQ ID NO (109) of Table 2.
Aspect 17. The method of aspect 14, wherein the first negative affinity reagent comprises an antibody or aptamer that binds to a protein product of gene Y6 of Table 2.
Aspect 18. The method of aspect 14, wherein the first negative affinity reagent comprises an antibody or aptamer that binds to one or more of SEQ ID NO (66), SEQ ID NO (67) or SEQ ID NO (68).
Aspect 19. The method of any of aspects 14-18, wherein the first negative affinity reagent comprises an antibody or aptamer that is coupled to a solid matrix or a label.
Aspect 20. A composition enriched with human corneal endothelial cells that is made by the method of any of aspects 1-19.
Aspect 21. A kit comprising (a) a positive affinity reagent that selectively binds to human corneal endothelial cells relative to cells other than human corneal endothelial cells and (b) a negative affinity reagent that selectively binds to cells other than human corneal endothelial cells relative to human corneal endothelial cells.
Aspect 22. The kit of aspect 21, wherein the positive affinity reagent selectively binds to a corneal protein selected from protein products of genes X1 through X26 of Table 2, and may comprise, for example, an antibody or aptamer that binds to a corneal protein selected from protein products of genes X1 through X26 of Table 2, including an antibody or aptamer that binds to one or more proteins selected from SEQ ID NO (1) through SEQ ID NO (58) of Table 2.
Aspect 23. The kit of aspect 21, wherein the first positive affinity reagent comprises an antibody or aptamer that binds to a protein product of gene X5 of Table 2, an antibody or aptamer that binds to a protein product of gene X15 of Table 2, or an antibody or aptamer that binds to a protein product of X25 of Table 2.
Aspect 24. The kit of aspect 21, wherein the first positive affinity reagent comprises (a) an antibody or aptamer that binds to SEQ ID NO (8), (b) an antibody or aptamer that binds to one or more of SEQ ID NO (27), SEQ ID NO (28), SEQ ID NO (29), SEQ ID NO (30) or SEQ ID NO (31), or (c) an antibody or aptamer that binds to one or more of SEQ ID NO (53), SEQ ID NO (54), SEQ ID NO (55), SEQ ID NO (56) or SEQ ID NO (57).
Aspect 25. The kit of any of aspects 21-24, wherein the positive affinity reagent comprises an antibody or aptamer that is coupled to a solid matrix or a label.
Aspect 26. The kit of any of aspects 21-25, wherein the negative affinity reagent selectively binds to a corneal protein selected from protein products of genes Y1 through Y23 of Table 2, and may comprise, for example, an antibody or aptamer that binds to a corneal protein selected from protein products of genes Y1 through Y23 of Table 2, including an antibody or aptamer that binds to one or more proteins selected from SEQ ID NO (59) through SEQ ID NO (96) of Table 2, or wherein the negative affinity reagent selectively binds to a corneal protein selected from protein products of genes Z1 through Z8 of Table 2, and may comprise, for example, an antibody or aptamer that binds to a corneal protein selected from protein products of genes Z1 through Z8 of Table 2, including an antibody or aptamer that binds to one or more proteins selected from SEQ ID NO (97) through SEQ ID NO (109) of Table 2.
Aspect 27. The kit of any of aspects 21-25, wherein the negative affinity reagent comprises an antibody or aptamer that binds to a protein product of gene Y6 of Table 2.
Aspect 28. The kit of any of aspects 21-25, wherein the first negative affinity reagent comprises an antibody or aptamer that binds to one or more of SEQ ID NO (66), SEQ ID NO (67) or SEQ ID NO (68).
Aspect 29. The kit of any of aspects 21-28, wherein the negative affinity reagent comprises an antibody or aptamer that is coupled to a solid matrix or a label.
Aspect 30. An affinity reagent selected from (a) a solid matrix or a label coupled to an antibody or aptamer that binds to a corneal protein selected from protein products of genes X1 through X26 of Table 2, including an antibody or aptamer that binds to one or more proteins selected from SEQ ID NO (1) through SEQ ID NO (58) of Table 2, and (b) a solid matrix or a label coupled to an antibody or aptamer that binds to a corneal protein selected from protein products of genes Y1 through Y23 of Table 2, including an antibody or aptamer that binds to one or more proteins selected from SEQ ID NO (59) through SEQ ID NO (96) of Table 2, and protein products of genes Z1 through Z8 of Table 2, including an antibody or aptamer that binds to one or more proteins selected from SEQ ID NO (97) through SEQ ID NO (109) of Table 2.
Aspect 31. A composition enriched with human corneal endothelial cells comprising: (a) human corneal cells and (b) a first positive affinity reagent that selectively binds to human corneal endothelial cells relative to cells other than human corneal endothelial cells.
Aspect 32. The composition of aspect 31, comprising human corneal endothelial cells to which the first positive affinity reagent is bound.
Aspect 33. The composition of any of aspects 31-32, wherein the first positive affinity reagent selectively binds to human corneal endothelial cells relative to corneal keratocytes, human corneal endothelial cells of lower utility, or both.
Aspect 34. The composition of any of aspects 31-33, wherein the first positive affinity reagent selectively binds to a corneal protein selected from protein products of genes X1 through X26 of Table 2, and may comprise, for example, an antibody or aptamer that binds to a corneal protein selected from protein products of genes X1 through X26 of Table 2, including an antibody or aptamer that binds to one or more proteins selected from SEQ ID NO (1) through SEQ ID NO (58) of Table 2.
Aspect 35. The composition of any of aspects 31-33, wherein the first positive affinity reagent comprises an antibody or aptamer that binds to a protein product of gene X5 of Table 2, an antibody or aptamer that binds to a protein product of gene X15 of Table 2, or an antibody or aptamer that binds to a protein product of X25 of Table 2.
Aspect 36. The composition of any of aspects 31-33, wherein the first positive affinity reagent comprises (a) an antibody or aptamer that binds to SEQ ID NO (8), (b) an antibody or aptamer that binds to one or more of SEQ ID NO (27), SEQ ID NO (28), SEQ ID NO (29), SEQ ID NO (30) or SEQ ID NO (31), or (c) an antibody or aptamer that binds to one or more of SEQ ID NO (53), SEQ ID NO (54), SEQ ID NO (55), SEQ ID NO (56) or SEQ ID NO (57).
Aspect 37. The composition of any of aspects 31-36, wherein the first positive affinity reagent comprises an antibody or aptamer that is coupled to a label.
Aspect 38. The composition of aspect 37, wherein the label is selected from a magnetic label, a hapten (e.g., biotin) and a fluorescent label.
Aspect 39. The composition of any of aspects 31-38, further comprising a second positive affinity reagent that selectively binds to human corneal endothelial cells relative to cells other than human corneal endothelial cells, wherein the second positive affinity reagent differs from the first positive affinity reagent.
Aspect 40. The composition of aspect 39, comprising human corneal endothelial cells to which the second positive affinity reagent is bound.
Aspect 41. The composition of any of aspects 39-40, wherein the second positive affinity reagent selectively binds to a corneal protein selected from protein products of genes X1 through X26 of Table 2, and may comprise, for example, an antibody or aptamer that binds to a corneal protein selected from protein products of genes X1 through X26 of Table 2, including an antibody or aptamer that binds to one or more proteins selected from SEQ ID NO (1) through SEQ ID NO (58) of Table 2.
Aspect 42. The composition of any of aspects 39-40, wherein the second positive affinity reagent comprises an antibody or aptamer that binds to a protein product of gene X5 of Table 2, an antibody or aptamer that binds to a protein product of gene X15 of Table 2, or an antibody or aptamer that binds to a protein product of X25 of Table 2.
Aspect 43. The composition of any of aspects 39-40, wherein the second positive affinity reagent comprises (a) an antibody or aptamer that binds to SEQ ID NO (8), (b) an antibody or aptamer that binds to one or more of SEQ ID NO (27), SEQ ID NO (28), SEQ ID NO (29), SEQ ID NO (30) or SEQ ID NO (31), or (c) an antibody or aptamer that binds to one or more of SEQ ID NO (53), SEQ ID NO (54), SEQ ID NO (55), SEQ ID NO (56) or SEQ ID NO (57).
Aspect 44. The composition of any of aspects 39-43, wherein the second positive affinity reagent comprises a label.
Aspect 45. The composition of any of aspects 31-44, comprising a measurable amount of one or more negative affinity reagents that selectively binds to cells other than human corneal endothelial cells relative to human corneal endothelial cells.
Aspect 46. The composition of aspect 45, wherein the one or more negative affinity reagents selectively binds to corneal keratocytes, human corneal endothelial cells of lower utility, or both, relative to human corneal endothelial cells.
Aspect 47. The composition of any of aspects 45-46, wherein the one or more negative affinity reagents selectively bind to a corneal protein selected from protein products of genes Y1 through Y23 of Table 2, and may comprise, for example, one or more antibodies that bind to one or more corneal proteins selected from protein products of genes Y1 through Y23 of Table 2, including one or more antibodies or aptamers that bind to one or more proteins selected from SEQ ID NO (59) through SEQ ID NO (96) of Table 2, or the one or more negative affinity reagents selectively bind to a corneal protein selected from protein products of genes Z1 through Z8 of Table 2, and may comprise, for example, one or more antibodies or aptamers that bind to one or more corneal proteins selected from protein products of genes Z1 through Z8 of Table 2, including one or more antibodies or aptamers that bind to one or more proteins selected from SEQ ID NO (97) through SEQ ID NO (109) of Table 2.
Aspect 48. The composition of any of aspects 45-46, wherein the one or more negative affinity reagents comprise an antibody or aptamer that binds to a protein product of gene Y6 of Table 2.
Aspect 49. The composition of any of aspects 45-46, wherein the one or more negative affinity reagents comprise an antibody or aptamer that binds to one or more of SEQ ID NO (66), SEQ ID NO (67) or SEQ ID NO (68).
Aspect 50. The composition of any of aspects 45-49, wherein the one or more negative affinity reagents comprise an antibody or aptamer that is coupled to a label.
These and various other aspects and embodiments and as well as various advantages of the present invention will become immediately apparent to those of ordinary skill in the art upon review of the Detailed Description and appended claims to follow.



BRIEF DESCRIPTION OF THE DRAWINGS
FIGS. 1A-1C are bright field micrographs of HCECs and keratocytes in culture, with FIG. 1A illustrating a HCEC culture having preserved a typical cobblestone morphology (P2-HCEC-Good), FIG. 1B illustrating a HCEC culture having become fibroblastic (P3-HCEC-Fibroblastic), and FIG. 1C illustrating a human corneal keratocyte culture (P2-HCEC-Keratocytes).
FIG. 2 illustrates in bar graph form expression of four surface markers in different corneal cell populations analyzed by flow cytometry.
FIGS. 3A-3C are dual-color fluorescence histograms of HCECs and keratocytes. These dot plots show the differential expression of two surface markers (CD56:CD166, CD56:CD90 and CD56:CAR) in each cell population, with FIG. 3A corresponding to the P2-HCEC-Good culture shown in FIG. 1A, FIG. 3B corresponding to the P3-HCEC-Fibroblastic culture shown in FIG. 1B, and FIG. 3C corresponding to the P2-HCEC-Keratocytes culture shown in FIG. 1C.
FIGS. 4A-4D present Fluorescence profiles illustrating expression of four surface markers, specifically, CD56 (FIG. 4A), CAR (FIG. 4B), CD109 (FIG. 4C) and CD248 (FIG. 4D) in three different HCEC populations, specifically, canonical (good) HCECs, mixed (canonical and fibroblastic) HCECs, and fibroblastic (HCECs), analyzed by flow cytometry.
FIG. 5 illustrates in bar graph form expression of four surface markers in three different HCEC populations analyzed by flow cytometry.
FIGS. 6A-6C illustrates dual-color fluorescence histograms for various pairs of surface markers, specifically, CD56:CD248 (FIG. 6A), CD56:CD109 (FIG. 6B) and CD56:CAR (FIG. 6C) in three different HCEC populations, specifically, canonical HCECs, mixed HCECs and fibroblastic HCECs.
FIG. 7 illustrates trans-endothelial electrical resistance (TEER) as a function of time for cell cultures of three different HCEC populations.



DETAILED DESCRIPTION
A more complete understanding of the present invention is available by reference to the following detailed description of numerous aspects and embodiments of the invention. The detailed description of the invention which follows is intended to illustrate but not limit the invention.
As noted above, in some aspects, the present disclosure pertains to positive selection processes in which cell populations containing human corneal cells are contacted with one or more positive affinity reagents that selectively bind to HCECs relative to cells other than HCECs (e.g., corneal keratocytes, etc.), including positive affinity reagents that selectively bind to HCECs that are likely to have a higher clinical efficacy relative to the general HCEC population
In other aspects, the present disclosure pertains to negative selection processes in which cell populations containing human corneal cells are contacted with one or more negative affinity reagents that bind selectively bind to cells other than HCECs (e.g., corneal keratocytes, etc.) relative to HCECs.
These negative and positive selection methods may be used independently or in combination with one another, for example, to identifying HCECs, to isolate HCECs and/or to enrich cell populations with HCECs, among other uses.
Cell populations suitable for HCEC enrichment or isolation include those obtained from intact or residual human corneas, which may come, for instance, from embryonic, fetal, pediatric or adult tissue. For example, intact corneas may be subjected to a peel-off step in which the endothelium and its basement membrane (Descemet's membrane) are peeled off the stroma and collected. See Ko-Hua Chen et al., “Transplantation of Adult Human Corneal Endothelium Ex Vivo: A Morphologic Study,” Cornea 20(7): 731-737, 2001. In other embodiments, cell populations may be obtained from residual corneas (e.g., eye tissue remaining after a corneal button has been used for DSAEK).
Tissue from intact and residual corneas may be separated into individual cells by processes such as enzymatic and/or mechanical dissociation. At this step, cells are incubated for a period of time at room temperature or at 37° C. with a single enzyme or a combination of enzymes including some of the following: collagenase, papain, dispase, elastase, trypsin/EDTA, and/or DNAse. Later the tissues are mechanically dissociated using a conventional pipette or a glass pipette to obtain individual cells or cell clumps than can be then expanded in culture. See, e.g., Li W. et al., Invest Ophthalmol Vis Sci 2007; 48: 614; Ishino Y. et al., Invest Ophthalmol Vis Sci 2004; 45: 800; Chen K. H. et al., Cornea 2001; 20: 731.
The medium in which the cells may be suspended will be any medium which maintains the viability of HCECs. Various media are commercially available and may be used including Minimal Essential Medium (MEM), Dulbecco's Modified Eagle Medium (DMEM), Opti-MEMO, Media 199 or M199, Dulbecco's Modified Eagle Medium with Nutrient Mixture F-12 (DMEM/F-12), F99 Ham's F12, SHEM Ham's F12, EGM-2 endothelial growth medium frequently supplemented with serum of human or animal origin, BSA, HSA, growth factors, antioxidants, antibiotics, antimicotic agents, hormones, amino acids, and peptides. Specific examples of media are shown in Table 1 to follow.









TABLE 1





Base Medium
Serum
Growth Factors & Supplements







[M1]
10% 
2 ng/ml bFGF


DMEM

50 U/ml penicillin




50 μg/ml streptomycin


[M2]
8%
20 ng/ml NGF


Opti-MEM-I

5 ng/ml EGF




20 μg/ml ascorbic acid




200 mg/L calcium chloride




100 μg/ml pituitary extract




50 μg/ml gentamicin




1x antibiotic/antimycotic




0.08% chondroitin sulphate


[M3]
5%
0.5% DMSO


SHEM

2 ng/ml EGF


Ham's F12 & DMEM

5 μg/ml insulin


(1:1 ratio)

5 μg/ml transferrin




5 ng/ml selenium




0.5 μg/ml hydrocortisone




1 nM cholera toxin




50 μg/ml gentamicin




1.25 μg/ml amphotericin B


[M4]
5%
20 μg/ml ascorbic acid


F99

20 μg/ml bovine insulin


Ham's F 12 & M100

2.5 μg/mol transferrin


(1:1 ratio)

0.6 ng/ml sodium selentite




10 ng/ml bFGF









Cell cultures from intact and residual corneas contain unwanted contaminant cells which arise from residual non-endothelial tissue (e.g., stroma, epithelium, etc.) that may be present in the sample. In a culture of HCECs, HCECs that are of low cell transplant utility compared to other HCECs of high cell transplant utility may also be considered, in some fashion, “contaminants”.
Cell populations suitable for HCEC enrichment or isolation also include HCEC cultures in which contaminant cells have out-multiplied HCECs or in which HCECs have transformed spontaneously into other types of cells (e.g., keratocytes, etc.). As previously noted, contaminant cells such as keratocytes are particularly undesirable where it is desired to expand an HCEC culture ex vivo, because such cells grow faster than HCECs and can thus take over a cell culture.
Consequently, various aspects of the invention pertain to methods, reagents and kits for separation of HCECs from other cells, particularly, keratocytes and/or HCECs of lower utility. The HCECs are separated from mixtures of cells by techniques that select cells having particular characteristics.
Human corneal endothelial cells may identified or selected (a) through positive cell markers, which are cell markers that are found on the surfaces of HCECs but which are not found on the surfaces of contaminant cells which may be intermixed with HCECs (e.g., positive selection), (b) through negative cell markers, which are cell markers that are found on surfaces of contaminant cells that are intermixed with HCECs and but which are not found on the surfaces of HCECs (e.g., negative selection), and through a combination of positive and negative cell markers.
For example, in the case where whole human corneas are used as a source of endothelial cells, positive cell markers may be selected from corneal proteins which are found in the endothelium (which is formed from HCECs) but which are not found in other corneal tissue (i.e., the stroma and/or the epithelium). Conversely, negative cell markers may be selected from corneal proteins which are found in corneal tissue other than endothelium tissue (i.e., the stroma and/or the epithelium) but which are not found in corneal endothelium.
As another example, in the case where the source of endothelial cells is an endothelium and basement membrane that have been separated from the stroma and epithelium of an intact cornea, positive cell markers may be selected from corneal cell proteins which are found in the endothelium but which are not found in the stroma, while negative cell markers may be selected from corneal cell proteins which are found in the stroma but which are not found in corneal endothelium.
Corneal proteins which may be useful as cell markers in conjunction with the present invention include the suitable proteins selected from those presented in the Table 2 set forth in Appendix A.
Positive cell markers include suitable corneal proteins selected from protein products of genes X1-X26 in Table 2 (e.g., SEQ ID NO (1) through SEQ ID NO (58)) which are present in the corneal endothelium but are not present in the stroma or the epithelium.
Negative cell markers include (a) suitable corneal proteins selected from protein products of genes Y1-Y23 in Table 2 (e.g., SEQ ID NO (59) through SEQ ID NO (96)), which are present in the stroma and in epithelium but are not present in the endothelium and (b) suitable corneal proteins selected from protein products of genes Z1-Z8 in Table 2 (e.g., SEQ ID NO (97) through SEQ ID NO (109)), which are present in the stroma but are not present in the corneal endothelium (or epithelium).
As previously noted, in some aspects, the present disclosure pertains to (a) positive selection processes in which cell populations containing human corneal cells are contacted with one, two, three, four or more positive affinity reagents that selectively bind to HCECs relative to cells other than HCECs (e.g., corneal keratocytes, etc.), (b) negative selection processes in which cell populations containing human corneal cells are contacted with one, two, three, four or more negative affinity reagents that selectively bind to cells other than HCECs (e.g., corneal keratocytes, etc.) relative to HCECs, and (c) combinations of (a) and (b).
For this purpose, affinity reagents are employed which preferentially bind to various corneal proteins. Positive affinity reagents are those that preferentially bind to positive cell markers associated with HCECs while negative affinity reagents are those that preferably bind to negative cell markers associated with contaminant cells other than HCECs.
Various positive cell markers are described above and include corneal proteins which are found in the endothelium (which is formed from HCECs) but which are not found in other corneal tissue (i.e., the stroma and/or the epithelium). Various negative cell markers are also described above and include corneal proteins which are found in corneal tissue other than endothelium (i.e., the stroma and/or the epithelium) but which are not found in corneal endothelium.
Those skilled in the art will recognize that suitable negative and positive affinity reagents can be employed in any order and/or in any combination.
Affinity reagents suitable for use in the present disclosure may comprise any species which selectively binds to a given surface marker, including positive affinity reagents which selectively bind to positive cell markers and negative affinity reagents which selectively bind to negative cell markers.
Especially useful affinity reagents for the practice of the invention are antibodies (also referred to herein as “affinity antibodies”), nucleic acid aptamers and other engineered forms of protein scaffolds. Antibodies include whole antibodies and antibody fragments, e.g. Fab, F(ab′)2, light or heavy chain fragments, etc.
Affinity antibodies selected for use will have a low level of non-specific interactions.
Affinity antibodies may be polyclonal or monoclonal and, where not commercially available, may be readily produced by techniques known to those skilled in the art.
For instance, affinity antibodies to a given corneal protein may be obtained by immunizing a xenogeneic immunocompetent mammalian host (including murine, rodentia, lagomorpha, ovine, porcine, bovine, etc.) with the corneal protein of interest. Immunizations are performed in accordance with conventional techniques, where the corneal proteins may be injected subcutaneously, intramuscularly, intraperitoneally, intravascularly, etc., over a course of one or more injections. After completion of the immunization schedule, the antiserum may be harvested in accordance with conventional methods to provide polygonal antisera specific for the corneal protein of interest. Lymphocytes may also be harvested from the appropriate lymphoid tissue, e.g. spleen, draining lymph node, etc., and fused with an appropriate fusion partner, for example, a myeloma line, producing a hybridoma secreting a specific monoclonal antibody. Screening clones of hybridomas for the antigenic specificity of interest is performed in accordance with conventional methods.
In numerous embodiments, affinity antibodies are coupled to a suitable substrate, for example, a label or a solid matrix. Labels include magnetic labels such as magnetic beads or micro or nanoparticles including superparamagnetic nanoparticles, which allow for ease of separation. Labels also include biotin, which binds with high affinity to avidin or streptavidin. Labels further include fluorochromes, which can be used with flow cytometry, e.g., fluorescence activated cell sorting (FACS), or the like, to allow for ease of separation of a particular cell type. Fluorescence activated cell sorters have varying degrees of sophistication, such as multiple color channels, low angle and obtuse light scattering detecting channels, impedance channels, etc. Fluorochromes include phycobiliproteins, e.g., phycoerythrin and allophycocyanins, fluorescein and Texas red, cy7 and cy5, among others. Multiple antibodies each with an affinity to a particular corneal protein may each be labeled with a different fluorochrome, to permit independent sorting (multi-color analyses) for each associated cell protein.
Cell selection may also be achieved by “panning” with an affinity antibody attached to a solid matrix, e.g. a plate, an immobilized bead, and so forth. For example, an affinity antibody that has specificity for a particular corneal protein may be bound to a solid matrix and corneal cells displaying that particular corneal protein can be captured by the immobilized antibody while the other cells remain in suspension and can be removed.
Any sorting technique may be employed which is not unduly detrimental to the viability of the selected cells. Combinations of the above techniques may be used.
The precise method for coupling an antibody to a given substrate (e.g., a label, solid matrix, etc.) is not critical to the practice of the present disclosure, and a number of alternatives are known in the art. For example, affinity antibodies may directly or indirectly be coupled to a substrate. Direct coupling to a substrate can be achieved by use of various chemical linking groups, as known in the art. For example, an antibody can be coupled to a substrate through side chain amino or sulfhydryl groups and heterofunctional cross-linking reagents. Many heterofunctional compounds are available for linking to various entities. Specific examples include 3-(2-pyridyldithio)propionic acid N-hydroxysuccinimide ester (SPDP) or 4-(N-maleimidomethyl)-cyclohexane-1-carboxylic acid N-hydroxysuccinimide ester (SMCC), which can react with a reactive sulfhydryl group on the antibody and a reactive amino group on the substrate.
Alternatively, affinity antibodies can be indirectly coupled to a substrate via a hapten or a secondary antibody. For instance, the antibody may be directly conjugated to a hapten, and hapten-specific binding species may be conjugated to the substrate. Suitable haptens include digoxin, digoxigenin, FITC, dinitrophenyl, nitrophenyl, avidin, streptavidin, biotin, etc. For example, an antibody may be coupled to one member of a high affinity binding system (e.g., biotin) and another member of the high affinity binding system (e.g., avidin) attached to a substrate. Methods for conjugation of a hapten to a protein are known in the art, and kits for such conjugations are commercially available. The secondary antibody may be directly or indirectly bound to the substrate.
During cell separation, coupled antibodies may be combined with a suspension of cells and incubated for a period of time sufficient for the antibodies to bind to proteins on the cells. The amount of antibody necessary to bind a particular cell subset may be empirically determined by performing a test separation and analysis. The cells and antibodies are incubated for a period of time sufficient for binding to occur.
The medium in which the cells are separated will be any medium which maintains the viability of the cells. Various media are commercially available and include those listed above.
Coupled affinity antibodies include coupled positive affinity antibodies specific for the corneal proteins which are present on human corneal endothelial cells and which are not present on contaminant cells such as stromal and/or epithelial cells (for positive selection) and coupled negative affinity antibodies specific for corneal proteins which are present on contaminant cells such as stromal and/or epithelial cells and which are not present on human corneal endothelial cells (negative selection).
Once the antibody is bound to the cell, the bound cells are separated in accordance with the specific antibody preparation. For example, FACS separation may be used with fluorochrome labeled antibodies, immunomagnetic selection may be used with magnetic-labeled antibodies, “panning” may be employed with immobilized antibodies, and so forth.
Cells may be separated from affinity antibodies using known techniques, as desired. As a specific example, where an antibody in an immunopanning process is a positive selection antibody, the matrix with attached endothelial cells may be washed to remove unbound cells and the endothelial cells released using a suitable technique (e.g., trypsin digest).
While various specific embodiments employing antibodies as affinity reagents are specifically described herein, it is to be understood that other affinity reagents for binding positive or negative cell markers can be used in the same fashion, including nucleic acid aptamers and other engineered forms of protein scaffolds. Aptamers are synthetic oligonucleotides selected from pools of random-sequence oligonucleotides which bind to a wide range of biomolecular targets with high affinity and specificity. See, e.g., J. Wang and G. Li, “Aptamers against cell surface receptors: selection, modification and application,” Curr Med Chem. 2011; 18(27):4107-16.
The separated cells may be collected in any appropriate medium that maintains the viability of the cells.
Cell populations enriched with HCECs may thus be achieved in this manner. The HCEC population may constitute 50% or more of the cells in the cell composition, preferably at 75% or more of the cells in the cell composition, more preferably at 90% or more of the cells in the cell composition, and may be as many as 95% or more (e.g. substantially pure) of the cells in the cell population. Conversely, the cell populations may contain up to 50% of cells other than HCECs (e.g., corneal keratocytes, etc.), for instance 50% or less of such cells, preferably 25% or less of such cells, more preferably 10% or less of such cells, and may be as few as 5% or less of such cells.
The enriched cell population may be used immediately or stored. For example, at room temperature, at 4° C., at 37° C. or the cells may be frozen at liquid nitrogen temperatures and stored for long periods of time.
In certain embodiments, the enriched cells may be further expanded in vitro by adding culture media as described widely in the literature. See, e.g., Li W et al., Invest Ophthalmol Vis Sci 2007; 48: 614; Ishino Y et al., Invest Ophthalmol Vis Sci 2004; 45: 800; Chen K H et al., Cornea 2001; 20: 731.
The enriched HCEC compositions thus obtained have a variety of uses in clinical therapy, research, development, and commercial purposes.
For example, for therapeutic purposes, human corneal endothelial cells may be ocularly administered to an eye of a patient in order to treat corneal endothelial cell loss or dysfunction.
Other aspects of the invention pertain to kits for conducting cell separations as described herein. Such kits may include any combination of the following, among other elements: (a) one, two, three or more positive affinity reagents, each of which may be, for example, in the form of a positive affinity antibody attached to a suitable substrate such as a solid matrix (e.g. a plate, immobilized bead, etc.) or label (e.g., magnetic label, fluorescent label, etc.), (b) one, two, three or more unlabeled positive affinity antibodies, which the end user could label using standard methods, choosing their preferred labels (e.g., fluorophores, haptens, etc.), (c) one, two, three or more negative affinity reagents, each of which may be, for example, in the form of a negative affinity antibody attached to a suitable substrate such as a solid matrix (e.g. a plate, immobilized bead, etc.) or label (e.g., magnetic label, fluorescent label, etc.), (d) or one, two, three or more unlabeled negative affinity antibodies, which the end user could label using standard methods, choosing their preferred labels (e.g., fluorophores, haptens, etc.); (e) a combination of (a) and (c); (f) a combination of (b) and (d); (g) packaging; (h) printed materials with one or more of the following: (i) storage information and (ii) instructions regarding how to use the materials contained in the kit (e.g., positive affinity reagents, negative affinity reagents, a combination of antibodies for sequential use, etc.).
Example 1
HCECs were isolated from cadaveric donor corneas (Tampa Lions Eye Bank) and cultured and expanded following the method described by Joyce and Zhu in Cornea. 2004 November; 23(8 Suppl):S8-S19. Briefly, the endothelium and Descemet's membrane were peeled off of the stroma and after overnight stabilization at 37° C. in Opti-MEM® media (Gibco, Life Technologies Corp, Carlsbad, Calif.), supplemented with 8% fetal bovine serum (FBS), they were incubated for 1 hr at 37° C. with ethylenediaminetetraacetic acid (EDTA) to loosen up the cell-cell interactions. Cells were then mechanically dissociated to obtain a single-cell suspension, they were seeded onto FNC-coated culture wells and labeled as “P0” (passage zero). After reaching confluency, they were trypsinized and further expanded into more wells to increase their number. After one or two rounds of expansion, cells were collected and incubated with different antibodies as indicated below. Keratocytes were also obtained from cadaveric donor corneas using the method described by Stramer et al. in “Monoclonal antibody (3G5)-defined ganglioside: cell surface marker of corneal keratocytes,” Invest. Ophthalmol. Vis. Sci. 2004 vol. 45 no. 3 807-812. While one of the HCEC cultures preserved its typical cobblestone morphology at passage 2 (FIG. 1A), a second culture underwent endothelial-to-mesenchymal transition during passage 3 (P3) and the cells became fibroblastic (FIG. 1B). Such cells are generally referred to herein as human corneal endothelial cells of lower utility (e.g., HCECs that have undergone fibroblastic or mesenchymal transformation, etc.) The keratocyte culture exhibits the typical fibroblastic, elongated cell morphology (FIG. 1C).
HCECs from each culture and keratocytes were collected and incubated with one or more of the following labelled antibodies: (a) APC-CD56 which is a mouse monoclonal antibody against a protein product of gene X15 from Table 2 (referred herein to as CD56 surface protein) coupled to allophycocyanin (BD Biosciences, #555518), (b) PE-CD166, which is a mouse monoclonal antibody against a protein product of gene X1 from Table 2 (referred here to as CD166 surface protein) coupled to phycoerythin (BD Biosciences #559263), (c) FITC-CAR, which is a mouse monoclonal antibody against a protein product of gene X25 from Table 2 (referred to as CAR surface protein) coupled to fluorescein-5-Isothiocyanate (Santa Cruz Biotechnology, Santa Cruz, Calif., USA #sc-56892) and (d) PECy7-CD90, which is a mouse monoclonal antibody against a protein product of gene Z8 from Table 2 (referred to as CD90 surface protein) coupled to a tandem conjugate of PE (energy donor) which has an excitation wavelength of 565 nm and Cy7 (energy acceptor) which has an emission wavelength of 778 nm) (BD Biosciences #561558).
Expression of surface markers was analyzed using a BD LSRTMII flow cytometry system (BD Biosciences, San Jose, Calif.). The data shown in FIG. 2 are representative from one experiment. Similar results were obtained upon repeated experimentation. Quantification of the % positive cells for each marker shows that in fibroblastic cultures there is a decreased expression of CD56 and CAR, indicating that antibodies to these proteins may be used in conjunction with positive affinity reagents for “good” HCECs. A significant difference in the expression of CD166 or CD90 was not detected using this particular antibody.
FIGS. 3A-3C are dual-color fluorescence dot plots of the HCECs and keratocytes. These dot plots show the differential expression of two surface markers in each cell population as labeled. The percent of cells positive for an individual marker is shown in FIG. 2.
Example 2
HCECs were isolated from cadaveric donor corneas as described in Example 1. Also as discussed in Example 1, HCEC cultures were obtained (a) which evidenced a typical cobblestone morphology (referred to in this Example 2 as a “canonical” cell culture), (b) where all the cells had undergone an endothelial-to-mesenchymal transition (referred to in this Example as a “fibroblastic” cell culture) and (c) where some HCECs had undergone endothelial-to-mesenchymal transition (referred to in this Example as a “mixed” cell culture).
HCEC surface markers were identified by microarray data, and several with high expression in the endothelium (cultured and freshly dissected) but low expression in stroma were selected to be tested by flow cytometry analysis. In addition to APC-CD56, PE-CD166, FITC-CAR and PECy7-CD90 described in Example 1, also tested were (e) CD109-PE, (i.e., mouse anti-CD109), which is a monoclonal antibody against a protein product of gene Y6 from Table 2 (referred to as CD109 antigen) conjugated to phycoerythrin (PE), BD Biosciences Cat #556040 and (f) CD 248-BV, (i.e., mouse anti-Endosialin), which is an unconjugated monoclonal antibody against a protein product of gene X5 from Table 2 (referred to as CD248 antigen or Endosialin), (Millipore, Temecula, Calif., USA, Cat #MAB2626), incubated with Goat polyclonal anti-Mouse IgG secondary antibody conjugated to Brilliant Violent 421 (Biolegend, Inc., San Diego, Calif., USA, Cat #405317).
To address whether the expression of those markers in HCECs were affected by the fibroblastic conversion described above, HCEC cultures demonstrating two different morphologies (canonical and fibroblastic) and a corneal keratocyte culture as a control were immunostained for the surface proteins CD90, CAR, CD56 and CD166 (See Example 1, FIG. 2). CD56, CAR, CD109 and CD248 expression was also compared between canonical (good), mixed, and fibroblastic HCECs (see FIGS. 4A-4D and 5). Analysis of the percentage of cells expressing any of the individual markers in canonical and fibroblastic cultures demonstrated that CD56, CAR and CD248 expression was reduced in the fibroblastic culture (see FIG. 5), while CD109 was elevated (see FIG. 5); CD90 and CD166 expression did not significantly change between good/canonical and fibroblastic cultures (see Example 1, FIG. 2). A comparable trend was observed in the keratocyte culture used as control for CD90, CAR, CD56 and CD166 expression (see Example 1, FIG. 2).
Dot plot dual histograms of canonical, mixed and fibroblastic cultures shown in FIGS. 6A-6C demonstrated that canonical HCECs are predominantly CD56, CD248 and CAR positive, and CD109 negative; CD56 and CD248 expression is lost and CD109 expression increases as the culture becomes fibroblastic.
Finally, trans-endothelial electrical resistance (TEER) of cell cultures was measured. HCECs (a) from “good” or “canonical” cultures that expressed high levels of CD56, (b) from mixed cultures and (c) from fibroblastic cultures were plated onto inserts with 0.4 mm pores in 24-well culture plates (Transwell, Corning Costar, Acton, Mass.) at a density of 20,000 cells/insert and incubated in growth media as described in Example 1. TEER was measured using an EVOM volt-ohm meter with STX2 Electrode (World Precision Instrument, Inc., Sarasota, Fla.) for up to 65 days after initial plating. TEER measures the apical and basal plasma membrane resistance and the paracellular resistance and is used as an index of monolayer confluence integrity of tight junctions. To calculate final resistance (Ω·cm2), the resistance of blank filters were subtracted from those of filters with cells. Four wells per condition were averaged. HCECs exhibiting a canonical morphology and being CD56-positive demonstrated a superior barrier formation ability measured by TEER (FIG. 7).
Thus, we have identified a panel of surface makers that can be used to characterize a canonical and functionally superior HCEC culture, and may be used as quality control criteria or to potentially separate the best HCEC subpopulations for expansion.
Although various embodiments are specifically illustrated and described herein, it will be appreciated that modifications and variations of the present invention are covered by the above teachings and are within the purview of any appended claims without departing from the spirit and intended scope of the invention.
APPENDIX A








TABLE 2







Gene ID:
X1


Gene symbol:
ALCAM


Gene description:
activated leukocyte cell adhesion molecule


Unigene:
Hs.591293


Genbank:
DQ486139


Entrez Gene:
214


Refseq:
NM_001627







Protein sequence (SEQ ID NO (1)):








1
meskgasscr llfcllisat vfrpglgwyt vnsaygdtii ipcrldvpqn lmfgkwkyek


61
pdgspvfiaf rsstkksvqy ddvpeykdrl nlsenytlsi snarisdekr fvcmlvtedn


121
vfeaptivkv fkqpskpeiv skalfleteq lkklgdcise dsypdgnitw yrngkvlhpl


181
egavviifkk emdpvtqlyt mtstleyktt kadiqmpftc svtyygpsgq ktihseqavf


241
diyypteqvt iqvlppknai kegdnitlkc lgngnpppee flfylpgqpe girssntytl


301
tdvrrnatgd ykcslidkks miastaitvh yldlslnpsg evtrqigdal pvsctisasr


361
natvvwmkdn irlrsspsfs slhyqdagny vcetalqeve glkkresltl ivegkpqikm


421
tkktdpsgls ktiichvegf pkpaiqwtit gsgsvinqte espyingryy skiiispeen


481
vtltctaenq lertvnslnv saisipehde adeisdenre kvndqakliv givvglllaa


541
lvagvvywly mkksktaskh vnkdlgnmee nkkleennhk tea


 


Gene ID:
X2


Gene symbol:
ATP1A1


Gene description:
sodium/potassium-transporting ATPase subunit alpha-1


Unigene:
Hs.371889


Genbank:
BC003077


Entrez Gene:
476


Refseq:
NM_000701 | NM_001160233 | NM_001160234







Protein sequence isoform a (SEQ ID NO (2)):








1
mgkgvgrdky epaayseqgd kkgkkgkkdr dmdelkkevs mddhklslde lhrkygtdls


61
rgltsaraae ilardgpnal tpppttpewi kfcrqlfggf smllwigail cflaysiqaa


121
teeepqndnl ylgvvlsavv iitgcfsyyq eaksskimes fknmvpqqal virngekmsi


181
naeevvvgdl vevkggdrip adlriisang ckvdnssltg esepqtrspd ftnenpletr


241
niaffstncv egtargivvy tgdrtvmgri atlasglegg qtpiaaeieh fihiitgvav


301
flgvsffils lileytwlea vifligiiva nvpegllatv tvcltltakr marknclvkn


361
leavetlgst sticsdktgt ltqnrmtvah mwfdnqihea dttenqsgvs fdktsatwla


421
lsriaglcnr avfqanqenl pilkravagd asesallkci elccgsvkem reryakivei


481
pfnstnkyql sihknpntse pqhllvmkga perildrcss illhgkeqpl deelkdafqn


541
aylelgglge rvlgfchlfl pdeqfpegfq fdtddvnfpi dnlcfvglis midppraavp


601
davgkcrsag ikvimvtgdh pitakaiakg vgiisegnet vediaarlni pvsqvnprda


661
kacvvhgsdl kdmtseqldd ilkyhteivf artspqqkli ivegcqrqga ivavtgdgvn


721
dspalkkadi gvamgiagsd vskqaadmil lddnfasivt gveegrlifd nlkksiaytl


781
tsnipeitpf lifiianipl plgtvtilci dlgtdmvpai slayeqaesd imkrqprnpk


841
tdklvnerli smaygqigmi qalggfftyf vilaengflp ihllglrvdw ddrwindved


901
sygqqwtyeq rkiveftcht affvsivvvq wadlvicktr rnsvfqqgmk nkilifglfe


961
etalaaflsy cpgmgvalrm yplkptwwfc afpysllify ydevrkliir rrpggwveke


1021
tyy


 







Protein sequence isoform c (SEQ ID NO (3)):








1
mafkvgrdky epaayseqgd kkgkkgkkdr dmdelkkevs mddhklslde lhrkygtdls


61
rgltsaraae ilardgpnal tpppttpewi kfcrqlfggf smllwigail cflaysiqaa


121
teeepqndnl ylgvvlsavv iitgcfsyyq eaksskimes fknmvpqqal virngekmsi


181
naeevvvgdl vevkggdrip adlriisang ckvdnssltg esepqtrspd ftnenpletr


241
niaffstncv egtargivvy tgdrtvmgri atlasglegg qtpiaaeieh fihiitgvav


301
flgvsffils lileytwlea vifligiiva nvpegllatv tvcltltakr marknclvkn


361
leavetlgst sticsdktgt ltqnrmtvah mwfdnqihea dttenqsgvs fdktsatwla


421
lsriaglcnr avfqanqenl pilkravagd asesallkci elccgsvkem reryakivei


481
pfnstnkyql sihknpntse pqhllvmkga perildrcss illhgkeqpl deelkdafqn


541
aylelgglge rvlgfchlfl pdeqfpegfq fdtddvnfpi dnlcfvglis midppraavp


601
davgkcrsag ikvimvtgdh pitakaiakg vgiisegnet vediaarlni pvsqvnprda


661
kacvvhgsdl kdmtseqldd ilkyhteivf artspqqkli ivegcqrqga ivavtgdgvn


721
dspalkkadi gvamgiagsd vskqaadmil lddnfasivt gveegrlifd nlkksiaytl


781
tsnipeitpf lifiianipl plgtvtilci dlgtdmvpai slayeqaesd imkrqprnpk


841
tdklvnerli smaygqigmi qalggfftyf vilaengflp ihllglrvdw ddrwindved


901
sygqqwtyeq rkiveftcht affvsivvvq wadlvicktr rnsvfqqgmk nkilifglfe


961
etalaaflsy cpgmgvalrm yplkptwwfc afpysllify ydevrkliir rrpggwveke


1021
tyy


 







Protein sequence isoform d (SEQ ID NO (4)):








1
mdelkkevsm ddhklsldel hrkygtdlsr gltsaraaei lardgpnalt pppttpewik


61
fcrqlfggfs mllwigailc flaysiqaat eeepqndnly lgvvlsavvi itgcfsyyqe


121
aksskimesf knmvpqqalv irngekmsin aeevvvgdlv evkggdripa dlriisangc


181
kvdnssltge sepqtrspdf tnenpletrn iaffstncve gtargivvyt gdrtvmgria


241
tlasgleggq tpiaaeiehf ihiitgvavf lgvsffilsl ileytwleav ifligiivan


301
vpegllatvt vcltltakrm arknclvknl eavetlgsts ticsdktgtl tqnrmtvahm


361
wfdnqihead ttenqsgvsf dktsatwlal sriaglcnra vfqangenlp ilkravagda


421
sesallkcie lccgsvkemr eryakiveip fnstnkyqls ihknpntsep qhllvmkgap


481
erildrcssi llhgkeqpld eelkdafqna ylelgglger vlgfchlflp deqfpegfqf


541
dtddvnfpid nlcfvglism idppraavpd avgkcrsagi kvimvtgdhp itakaiakgv


601
giisegnetv ediaarlnip vsqvnprdak acvvhgsdlk dmtseqlddi lkyhteivfa


661
rtspqqklii vegcqrqgai vavtgdgvnd spalkkadig vamgiagsdv skqaadmill


721
ddnfasivtg veegrlifdn lkksiaytlt snipeitpfl ifiianiplp lgtvtilcid


781
lgtdmvpais layeqaesdi mkrqprnpkt dklvnerlis maygqigmiq alggfftyfv


841
ilaengflpi hllglrvdwd drwindveds ygqqwtyeqr kiveftchta ffvsivvvqw


901
adlvicktrr nsvfqqgmkn kilifglfee talaaflsyc pgmgvalrmy plkptwwfca


961
fpysllifvy devrkliirr rpggwveket yy


 


Gene ID:
X3


Gene symbol:
CD200


Gene description:
CD 200 molecule


Unigene:
Hs.79015


Genbank:
AK297194 | AF063591 | BC022522 | BC031103 | AY603771 | AK293399


Entrez Gene:
4345


Refseq:
NM_001004196 | NM_005944







Protein sequence isoform b (SEQ ID NO (5)):








1
merltltrti ggplltatll gkttindyqv irmpfshlst yslvwvmaav vlctaqvqw


61
tqdereqlyt paslkcslqn aqealivtwq kkkayspenm vtfsenhgvv iqpaykdkin


121
itqlglqnst itfwnitled egcymclfnt fgfgkisgta cltvyvqpiv slhykfsedh


181
lnitcsatar papmvfwkvp rsgienstvt lshpngttsv tsilhikdpk nqvgkevicq


241
vlhlgtvtdf kqtvnkgywf svplllsivs lvillvlisi llywkrhrnq drep


 







Protein sequence isoform a (SEQ ID NO (6)):








1
merlvirmpf shlstyslvw vmaavvlcta qvqvvtqder eqlytpaslk cslqnaqeal


61
ivtwqkkkav spenmvtfse nhgvviqpay kdkinitqlg lqnstitfwn itledegcym


121
clfnifgfgk isgtacltvy vqpivslhyk fsedhlnitc satarpapmv fwkvprsgie


181
nstvtlshpn gttsvtsilh ikdpknqvgk evicqvlhlg tvtdfkqtvn kgywfsvpll


241
lsivslvill vlisillywk rhrnqdrep


 


Gene ID:
X4


Gene symbol:
LAMB1


Gene description:
laminin, beta 1


Unigene:
Hs.650585


Genbank:
M61916


Entrez Gene:
3912


Refseq:
NM__002291







Protein sequence (SEQ ID NO (7)):








1
mgllqllafs flalcrarvr aqepefsygc aegscypatg dlligraqkl svtstcglhk


61
pepycivshl qedkkcficn sqdpyhetln pdshlienvv ttfapnrlki wwqsengven


121
vtiqldleae fhfthlimtf ktfrpaamli erssdfgktw gvyryfaydc easfpgistg


181
pmkkvddiic dsrysdieps tegevifral dpafkiedpy spriqnllki tnlrikfvkl


241
htlgdnllds rmeirekyyy avydmvvrgn cfcyghasec apvdgfneev egmvhghcmc


301
rhntkglnce lcmdfyhdlp wrpaegrnsn ackkcncneh sischfdmav ylatgnvsgg


361
vcddcqhntm grnceqckpf yyqhperdir dpnfcerctc dpagsqnegi cdsytdfstg


421
liagqcrckl nvegehcdvc kegfydlsse dpfgckscac nplgtipggn pcdsetghcy


481
ckrlvtgqhc dqclpehwgl sndldgcrpc dcdlggalnn scfaesgqcs crphmigrqc


541
nevepgyyfa tldhylyeae eanlgpgvsi verqyiqdri pswtgagfvr vpegayleff


601
idnipysmey diliryepql pdhwekavit vqrpgripts srcgntipdd dnqvvslspg


661
sryvvlprpv cfekgtnytv rlelpqytss dsdvespytl idslvlmpyc ksldiftvgg


721
sgdgvvtnsa wetfqryrcl ensrsvvktp mtdvcrniif sisallhqtg lacecdpqgs


781
lssvcdpngg qcqcrpnvvg rtcnrcapgt fgfgpsgckp cechlqgsvn afcnpvtgqc


841
hcfqgvyarq cdrclpghwg fpscqpcqcn ghaddcdpvt geclncqdyt mghncercla


901
gyygdpiigs gdhcrpcpcp dgpdsgrqfa rscyqdpvtl qlacvcdpgy igsrcddcas


961
gyfgnpsevg gscqpcqchn nidttdpeac dketgrclkc lyhtegehcq fcrfgtygda


1021
lqqdcrkcvc nylgtvqehc ngsdcqcdka tgqclclpnv igqncdreap ntwqlasgtg


1081
cdpcncnaah sfgpscneft gqcqcmpgfg grtcsecqel fwgdpdvecr acdcdprgie


1141
tpqcdqstgq cvcvegvegp rcdkctrgys gvfpdctpch qcfalwdvii aeltnrthrf


1201
lekakalkis gvigpyretv dsverkvsei kdilaqspaa eplknignlf eeaeklikdv


1261
temmaqvevk lsdttsqsns takeldslqt eaesldntvk elaeqlefik nsdirgalds


1321
itkyfqmsle aeervnastt epnstveqsa lmrdrvedvm meresqfkek qeeqarllde


1381
lagklqsldl saaaemtcgt ppgascsete cggpncrtde gerkcggpgc gglvtvahna


1441
wqkamdldqd vlsalaeveq lskmvseakl radeakqsae dillktnatk ekmdksneel


1501
rnlikqirnf ltqdsadlds ieavanevlk mempstpqql qnltedirer veslsqvevi


1561
lqhsaadiar aemlleeakr asksatdvkv tadmvkeale eaekaqvaae kaikqadedi


1621
qgtqnlltsi esetaaseet lfnasqrise lernveelkr kaaqnsgeae yiekvvytvk


1681
qsaedvkktl dgeldekykk venliakkte esadarrkae mlqneaktll aqansklqll


1741
kdlerkyedn qryledkaqe larlegevrs llkdisqkva vystcl


 


Gene ID:
X5


Gene symbol:
CD248


Gene description:
endosialin


Unigene:
Hs.195727


Genbank:
AF279142


Entrez Gene:
57124


Refseq:
NM_020404







Protein sequence (SEQ ID NO (8)):








1
mllrlllawa aagptlgqdp waaepraacg psscyalfpr rrtfleawra crelggdlat


61
prtpeeaqrv dslvgagpas rllwiglqrq arqcqlqrpl rgftwttgdq dtaftnwaqp


121
asggpcpaqr cvaleasgeh rwlegsctla vdgylcqfgf egacpalqde agqagpavyt


181
tpfhlvstef ewlpfgsvaa vqcqagrgas llcvkqpegg vgwsragplc lgtgcspdng


241
gcehecveev dghvscrcte gfrlaadgrs cedpcaqapc eqqcepggpq gyschcrlgf


301
rpaeddphrc vdtdecqiag vcqqmcvnyv ggfecycseg heleadgisc spagamgaqa


361
sqdlgdelld dgedeedede awkafnggwt empgilwmep tqppdfalay rpsfpedrep


421
qipypeptwp pplsaprvpy hssvlsvtrp vvvsathptl psahqppvip athpalsrdh


481
qipviaanyp dlpsayqpgi lsvshsaqpp ahqppmistk ypelfpahqs pmfpdtrvag


541
tqttthlpgi ppnhaplvtt lgaqlppqap dalvlrtqat qlpiiptaqp sltttsrspv


601
spahqisvpa atqpaalptl lpsqsptnqt spispthphs kapqipredg pspklalwlp


661
spaptaapta lgeaglaehs qrddrwllva llvptcvflv vllalgivyc trcgphapnk


721
ritdcyrwvi hagsksptep mpprgsltgv qtcrtsv


 


Gene ID:
X6


Gene symbol:
COL4A6


Gene description:
collagen, type IV, alpha 6


Unigene:
Hs.145586


Genbank:
D21337


Entrez Gene:
1288


Refseq:
NM_033641 | NM_001847







Protein sequence isoform b (SEQ ID NO (9)):








1
mhpglwlllv tlclteelaa ageksygkpc ggqdcsgscq cfpekgargr pgpigiqgpt


61
gpqgftgstg lsglkgergf pgllgpygpk gdkgpmgvpg flgingipgh pgqpgprgpp


121
gldgcngtqg avgfpgpdgy pgllgppglp gqkgskgdpv lapgsfkgmk gdpglpgldg


181
itgpqgapgf pgavgpagpp glqgppgppg plgpdgnmgl gfqgekgvkg dvglpgpagp


241
ppstgelefm gfpkgkkgsk gepgpkgfpg isgppgfpgl gttgekgekg ekgipglpgp


301
rgpmgsegvq gppgqqgkkg tlgfpglngf qgiegqkgdi glpgpdvfid idgavisgnp


361
gdpgvpglpg lkgdegiqgl rgpsgvpglp alsgvpgalg pqgfpglkgd qgnpgrttig


421
aaglpgrdgl pgppgppgpp spefetetlh nkesgfpglr geqgpkgnlg lkgikgdsgf


481
cacdggvpnt gppgepgppg pwgliglpgl kgargdrgsg gaqgpagapg lvgplgpsgp


541
kgkkgepils tiqgmpgdrg dsgsqgfrgv igepgkdgvp glpglpglpg dggqgfpgek


601
glpglpgekg hpgppglpgn glpglpgprg lpgdkgkdgl pgqqglpgsk gitlpciipg


661
sygpsgfpgt pgfpgpkgsr glpgtpgqpg ssgskgepgs pglvhlpelp gfpgprgekg


721
1pgfpglpgk dglpgmigsp glpgskgatg difgaengap geqglqgltg hkgflgdsgl


781
pglkgvhgkp gllgpkgerg spgtpgqvgq pgtpgssgpy gikgksglpg apgfpgisgh


841
pgkkgtrgkk gppgsivkkg lpglkglpgn pglvglkgsp gspgvaglpa lsgpkgekgs


901
vgfvgfpgip glpgipgtrg lkgipgstgk mgpsgragtp gekgdrgnpg pvgipsprrp


961
msnlwlkgdk gsqgsagsng fpgprgdkge agrpgppglp gapglpgiik gvsgkpgppg


1021
fmgirglpgl kgssgitgfp gmpgesgsqg irgspglpga sglpglkgdn gqtveisgsp


1081
gpkgqpgesg fkgtkgrdgl ignigfpgnk gedgkvgvsg dvglpgapgf pgvagmrgep


1141
glpgssghqg aigplgspgl igpkgfpgfp glhglnglpg tkgthgtpgp sitgvpgpag


1201
lpgpkgekgy pgigigapgk pglrgqkgdr gfpglqgpag lpgapgislp sliagqpgdp


1261
grpgldgerg rpgpagppgp pgpssnqgdt gdpgfpgipg pkgpkgdqgi pgfsglpgel


1321
glkgmrgepg fmgtpgkvgp pgdpgfpgmk gkagprgssg lqgdpgqtpt aeavqvppgp


1381
lglpgidgip gltgdpgaqg pvglqgskgl pgipgkdgps glpgppgalg dpglpglqgp


1441
pgfegapgqq gpfgmpgmpg qsmrvgytlv khsqseqvpp cpigmsqlwv gysllfvegq


1501
ekahnqdlgf agsclprfst mpfiycnine vchyarrndk sywlsttapi pmmpvsqtqi


1561
pqyisrcsvc eapsqaiavh sqditipqcp lgwrslwigy sflmhtaaga egggqslvsp


1621
gscledfrat pfiecsgarg tchyfankys fwlttveerq qfgelpvset lkagqlhtrv


1681
srcqvcmksl


 







Protein sequence isoform a (SEQ ID NO (10)):








1
mlinklwlll vtlclteela aageksygkp cggqdcsgsc qcfpekgarg rpgpigiqgp


61
tgpqgftgst glsglkgerg fpgllgpygp kgdkgpmgvp gflgingipg hpgqpgprgp


121
pgldgcngtq gavgfpgpdg ypgllgppgl pgqkgskgdp vlapgsfkgm kgdpglpgld


181
gitgpqgapg fpgavgpagp pglqgppgpp gplgpdgnmg lgfqgekgvk gdvglpgpag


241
pppstgelef mgfpkgkkgs kgepgpkgfp gisgppgfpg lgttgekgek gekgipglpg


301
prgpmgsegv qgppgqqgkk gtlgfpglng fqgiegqkgd iglpgpdvfi didgavisgn


361
pgdpgvpglp glkgdegiqg lrgpsgvpgl palsgvpgal gpqgfpglkg dqgnpgrtti


421
gaaglpgrdg lpgppgppgp pspefetetl hnkesgfpgl rgeqgpkgnl glkgikgdsg


481
fcacdggvpn tgppgepgpp gpwgliglpg lkgargdrgs ggaqgpagap glvgplgpsg


541
pkgkkgepil stiqgmpgdr gdsgsqgfrg vigepgkdgv pglpglpglp gdggqgfpge


601
kglpglpgek ghpgppglpg nglpglpgpr glpgdkgkdg lpgqqglpgs kgitlpciip


661
gsygpsgfpg tpgfpgpkgs rglpgtpgqp gssgskgepg spglvhlpel pgfpgprgek


721
glpgfpglpg kdglpgmigs pglpgskgat gdifgaenga pgeqglqglt ghkgflgdsg


781
lpglkgvhgk pgllgpkger gspgtpgqvg qpgtpgssgp ygikgksglp gapgfpgisg


841
hpgkkgtrgk kgppgsivkk glpglkglpg npglvglkgs pgspgvaglp alsgpkgekg


901
svgfvgfpgi pglpgipgtr glkgipgstg kmgpsgragt pgekgdrgnp gpvgipsprr


961
pmsnlwlkgd kgsqgsagsn gfpgprgdkg eagrpgppgl pgapglpgii kgvsgkpgpp


1021
gfmgirglpg lkgssgitgf pgmpgesgsq girgspglpg asglpglkgd ngqtveisgs


1081
pgpkgqpges gfkgtkgrdg lignigfpgn kgedgkvgvs gdvglpgapg fpgvagmrge


1141
pglpgssghq gaigplgspg ligpkgfpgf pglhglnglp gtkgthgtpg psitgvpgpa


1201
glpgpkgekg ypgigigapg kpglrgqkgd rgfpglqgpa glpgapgisl psliagqpgd


1261
pgrpgldger grpgpagppg ppgpssnqgd tgdpgfpgip gpkgpkgdqg ipgfsglpge


1321
lglkgmrgep gfmgtpgkvg ppgdpgfpgm kgkagprgss glqgdpgqtp taeavqvppg


1381
plglpgidgi pgltgdpgaq gpvglqgskg lpgipgkdgp sglpgppgal gdpglpglqg


1441
ppgfegapgq qgpfgmpgmp gqsmrvgytl vkhsqseqvp pcpigmsqlw vgysllfveg


1501
qekahnqdlg fagsclprfs tmpfiycnin evchyarrnd ksywlsttap ipmmpvsqtq


1561
ipqyisrcsv ceapsqaiav hsqditipqc plgwrslwig ysflmhtaag aegggqslvs


1621
pgscledfra tpfiecsgar gtchyfanky sfwlttveer qqfgelpvse tlkagqlhtr


1681
vsrcqvcmks l


 


Gene ID:
X7


Gene symbol:
PCDH7


Gene description:
protocadherin 7


Unigene:
Hs.479439 | Hs.724529


Genbank:
AB006755


Entrez Gene:
5099


Refseq:
NM_032456 | NM_002589 | NM_032457 | NM_001173523







Protein sequence isoform b (SEQ ID NO (11)):








1
mlrmrtagwa rgwelgccll lplslslaaa kqllryrlae egpadvrign vasdlgivtg


61
sgevtfsles gseylkidnl tgelstserr idreklpqcq mifdenecfl dfevsvigps


121
qswvdlfegq vivldindnt ptfpspvltl tveenrpvgt lyllptatdr dfgrngiery


181
ellqepgggg sggesrraga adsapypggg gngasgggsg gskrrldase ggggtnpggr


241
ssvfelqvad tpdgekqpql ivkgaldreq rdsyeltlrv rdggdpprss qailrvlitd


301
vndnsprfek svyeadlaen sapgtpilql raadldvgvn gqieyvfgaa tesvrrllrl


361
detsgwlsvl hridreevnq lrftvmardr gqppktdkat vvlnikdend nvpsieirki


421
griplkdgva nvaedvlvdt pialvqvsdr dqgengvvtc tvvgdvpfql kpasdtegdq


481
nkkkyflhts tpldyeatre fnvvivavds gspslssnns livkvgdtnd nppmfgqsvv


541
evyfpennip gervatvlat dadsgknaei aysldssvmg ifaidpdsgd ilvntvldre


601
qtdryefkvn akdkgipvlq gsttvivqva dkndndpkfm qdvftfyvke nlqpnspvgm


661
vtvmdadkgr naemslyiee nnnifsiend tgtiystmsf drehqttytf rvkavdggdp


721
prsatatvsl fvmdendnap tvtlpknisy tllppssnvr tvvatvlatd sddginadln


781
ysivggnpfk lfeidptsgv vslvgkltqk hyglhrlvvq vndsgqpsqs tttlvhvfvn


841
esvsnataid sqiarslhip ltqdiagdps yeiskqrlsi vigvvagimt viliilivvm


901
arycrsknkn gyeagkkdhe dfftpqqhdk skkpkkdkkn kkskqplyss ivtveaskpn


961
gqrydsvnek lsdspsmgry rsvnggpgsp dlarhyksss plptvqlhpq sptagkkhqa


1021
vqdlppantf vgagdnisig sdhcseyscq tnnkyskqvr cipnifkypr eg


 







Protein sequence isoform a (SEQ ID NO (12)):








1
mlrmrtagwa rgwelgccll lplslslaaa kqllryrlae egpadvrign vasdlgivtg


61
sgevtfsles gseylkidnl tgelstserr idreklpqcq mifdenecfl dfevsvigps


121
qsvwdlfegq vivldindnt ptfpspvltl tveenrpvgt lyllptatdr dfgrngiery


181
ellqepgggg sggesrraga adsapypggg gngasgggsg gskrrldase ggggtnpggr


241
ssvfelqvad tpdgekqpql ivkgaldreq rdsyeltlrv rdggdpprss qailrvlitd


301
vndnsprfek svyeadlaen sapgtpilql raadldvgvn gqieyvfgaa tesvrrllrl


361
detsgwlsvl hridreevnq lrftvmardr gqppktdkat vvlnikdend nvpsieirki


421
griplkdgva nvaedvlvdt pialvqvsdr dqgengvvtc tvvgdvpfql kpasdtegdq


481
nkkkyflhts tpldyeatre fnvvivavds gspslssnns livkvgdtnd nppmfgqsvv


541
evyfpennip gervatvlat dadsgknaei aysldssvmg ifaidpdsgd ilvntvldre


601
qtdryefkvn akdkgipvlq gsttvivqva dkndndpkfm qdvftfyvke nlqpnspvgm


661
vtvmdadkgr naemslyiee nnnifsiend tgtiystmsf drehqttytf rvkavdggdp


721
prsatatvsl fvmdendnap tvtlpknisy tllppssnvr tvvatvlatd sddginadln


781
ysivggnpfk lfeidptsgv vslvgkltqk hyglhrlvvq vndsgqpsqs tttlvhvfvn


841
esysnataid sqiarslhip ltqdiagdps yeiskqrlsi vigvvagimt viliilivvm


901
arycrsknkn gyeagkkdhe dfftpqqhdk skkpkkdkkn kkskqplyss ivtveaskpn


961
gqrydsvnek lsdspsmgry rsvnggpgsp dlarhyksss plptvqlhpq sptagkkhqa


1021
vqdlppantf vgagdnisig sdhcseyscq tnnkyskqmr lhpyitvfg


 







Protein sequence isoform c (SEQ ID NO (13)):








1
mlrmrtagwa rgwelgccll lplslslaaa kqllryrlae egpadvrign vasdlgivtg


61
sgevtfsles gseylkidnl tgelstserr idreklpqcq mifdenecfl dfevsvigps


121
qswvdlfegq vivldindnt ptfpspvltl tveenrpvgt lyllptatdr dfgrngiery


181
ellqepgggg sggesrraga adsapypggg gngasgggsg gskrrldase ggggtnpggr


241
ssvfelqvad tpdgekqpql ivkgaldreq rdsyeltlrv rdggdpprss qailrvlitd


301
vndnsprfek svyeadlaen sapgtpilql raadldvgvn gqieyvfgaa tesvrrllrl


361
detsgwlsvl hridreevnq lrftvmardr gqppktdkat vvlnikdend nvpsieirki


421
griplkdgva nvaedvlvdt pialvqvsdr dqgengvvtc tvvgdvpfql kpasdtegdq


481
nkkkyflhts tpldyeatre fnvvivavds gspslssnns livkvgdtnd nppmfgqsvv


541
evyfpennip gervatvlat dadsgknaei aysldssvmg ifaidpdsgd ilvntvldre


601
qtdryefkvn akdkgipvlq gsttvivqva dkndndpkfm qdvftfyvke nlqpnspvgm


661
vtvmdadkgr naemslyiee nnnifsiend tgtiystmsf drehqttytf rvkavdggdp


721
prsatatvsl fvmdendnap tvtlpknisy tllppssnvr tvvatvlatd sddginadln


781
ysivggnpfk lfeidptsgv vslvgkltqk hyglhrlvvq vndsgqpsqs tttlvhvfvn


841
esvsnataid sqiarslhip ltqdiagdps yeiskqrlsi vigvvagimt viliilivvm


901
arycrsknkn gyeagkkdhe dfftpqqhdk skkpkkdkkn kkskqplyss ivtveaskpn


961
gqrydsvnek lsdspsmgry rsvnggpgsp dlarhyksss plptvqlhpq sptagkkhqa


1021
vqdlppantf vgagdnisig sdhcseyscq tnnkyskqpf rrvtfsvvsq pqdphqgslq


1081
scydsglees etpssksssg prlgalplpe dnyerttpdg svdsrplpdv altgkctrec


1141
deyghsdscw mpvrtsperk ksqpklstfm pvdergsqek langeaaimg drnrnllnkk


1201
ltssyeffsa asfskneean pedipltktg eykpspvntl trrevyl


 







Protein sequence isoform d (SEQ ID NO (14)):








1
mlrmrtagwa rgwclgccll lplslslaaa kqllryrlae egpadvrign vasdlgivtg


61
sgevtfsles gseylkidni tgelstserr idreklpqcq mifdenecfl dfevsvigps


121
qswvdlfegq vivldindnt ptfpspvltl tveenrpvgt lyllptatdr dfgrngiery


181
ellqepgggg sggesrraga adsapypggg gngasgggsg gskrrldase ggggtnpggr


241
ssvfelqvad tpdgekqpql ivkgaldreq rdsyeltlrv rdggdpprss qailrvlitd


301
vndnsprfek svyeadlaen sapgtpilql raadldvgvn gqieyvfgaa tesvrrllrl


361
detsgwlsvl hridreevnq lrftvmardr gqppktdkat vvlnikdend nvpsieirki


421
griplkdgva nvaedvlvdt pialvqvsdr dqgengvvtc tvvgdvpfql kpasdtegdq


481
nkkkyflhts tpldyeatre fnvvivavds gspslssnns livkvgdtnd nppmfgqsvv


541
evyfpennip gervatvlat dadsgknaei aysldssvmg ifaidpdsgd ilvntvldre


601
qtdryefkvn akdkgipvlq gsttvivqva dkndndpkfm qdvftfyvke nlqpnspvgm


661
vtvmdadkgr naemslyiee nnnifsiend tgtiystmsf drehqttytf rvkavdggdp


721
prsatatvsl fvmdendnap tvtlpknisy tllppssnvr tvvatvlatd sddginadln


781
ysivggnpfk lfeidptsgv vslvgkltqk hyglhrlvvq vndsgqpsqs tttlvhvfvn


841
esvsnataid sqiarslhip ltqdiagdps yeiskqrlsi vigvvagimt viliilivvm


901
arycrsknkn gyeagkkdhe dfftpqqhdk skkpkkdkkn kkskqplyss ivtveaskpn


961
gqrydsvnek lsdspsmgry rsvnggpgsp dlarhyksss plptvqlhpq sptagkkhqa


1021
vqdlppantf vgagdnisig sdhcseyscq tnnkyskqpf rrylfsvvsq pqdphqgslq


1081
scydsglees etpssksssg prlgalplpe dnyerttpdg svgeaehmen dsrplpdval


1141
tgkctrecde yghsdscwmp vrtsperkks qpklstfmpv dergsqekla ngeaaimgdr


1201
nrnllnkklt ssyetfsaas fskneeanpe dipltktgey kpspvntltr revyl


 


Gene ID:
X8


Gene symbol:
NOG


Gene description:
noggin


Unigene:
Hs.248201


Genbank:
BC034027


Entrez Gene:
9241


Refseq:
NM_005450







Protein sequence (SEQ ID NO(15)):








1
mercpslgvt lyalvvvlgl ratpaggqhy lhirpapsdn lplvdliehp dpifdpkekd


61
lnetllrsll gghydpgfma tsppedrpgg gggaaggaed laeldqllrq rpsgampsei


121
kglefsegla qgkkqrlskk lrrklqmwlw sqtfcpvlya wndlgsrfwp ryvkvgscfs


181
krscsvpegm vckpsksvhl tvlrwrcqrr ggqrcgwipi qypiiseckc sc


 


Gene ID:
X9


Gene symbol:
SULF1


Gene description:
sulfatase 1


Unigene:
Hs.409602


Genbank:
AF545571


Entrez Gene:
23213


Refseq:
NM_001128205 | NM_015170 | NM_001128206 | NM_001128204







Protein sequence (SEQ ID NO(16)):








1
mkysccalvl avlgtellgs lcstvrsprf rgriqqerkn irpniilvlt ddqdvelgsl


61
qvmnktrkim ehggatfina fvttpmccps rssmltgkyv hnhnvytnne ncsspswqam


121
heprtfavyl nntgyrtaff gkylneyngs yippgwrewl gliknsrlyn ytvcrngike


181
khgfdyakdy ftdlitnesi nyfkmskrmy phrpvmmvis haaphgpeds apqfsklypn


241
asqhitpsyn yapnmdkhwi mqytgpmlpi hmeftnilqr krlqtlmsvd dsverlynml


301
vetgelenty iiytadhgyh igqfglvkgk smpydfdirv pffirgpsve pgsivpqivl


361
nidlaptild iagldtppdv dgksvlklld pekpgnrfrt nkkakiwrdt flvergkflr


421
kkeesskniq qsnhlpkyer vkelcqqary qtaceqpgqk wqciedtsgk lrihkckgps


481
dlltvrqstr nlyargfhdk dkecscresg yrasrsqrks qrqflrnqgt pkykprfvht


541
rqtrslsvef egeiydinle eeeelqvlqp rniakrhdeg hkgprdlqas sggnrgrmla


601
dssnavgppt tvrvthkcfi lpndsihcer elyqsarawk dhkayidkei ealqdkiknl


661
revrghlkrr kpeecscskq syynkekgvk kqeklkshlh pfkeaaqevd sklqlfkenn


721
rrrkkerkek rrqrkgeecs lpgltcfthd nnhwqtapfw nlgsfcacts snnntywclr


781
tvnethnflf cefatgfley fdmntdpyql tntvhtverg ilnqlhvqlm elrscqgykq


841
cnprpknldv gnkdggsydl hrgqlwdgwe g


 


Gene ID:
X10


Gene symbol:
SORT1


Gene description:
sortilin 1


Unigene:
Hs.485195


Genbank:
X98248


Entrez Gene:
6272


Refseq:
NM_002959 | NM_001205228







Protein sequence isoform 1 (SEQ ID NO (17)):








1
merpwgaadg lsrwphglgl llllqllpps tlsqdrldap pppaaplprw sgpigvswgl


61
raaaaggafp rggrwrrsap gedeecgrvr dfvaklannt hqhvfddlrg syslswvgds


121
tgvilvlttf hvplvimtfg qsklyrsedy gknfkditdl inntfirtef gmaigpensg


181
kvvltaevsg gsrggrifts sdfaknfvqt dlpfhpltqm myspqnsdyl lalstenglw


241
vsknfggkwe eihkavclak wgsdntifft tyangsckad lgalelwrts dlgksfktig


301
vkiysfglgg rflfasvmad kdttrrihvs tdqgdtwsma qlpsvgqeqf ysilaanddm


361
vfmhvdepgd tgfgtiftsd drgivysksl drhlytttgg etdftnvtsl rgvyitsvls


421
ednsiqtmit fdqggrwthl rkpensecda taknknecsl hihasysisq klnvpmapls


481
epnavgivia hgsvgdaisv mvpdvyisdd ggyswtkmle gphyytilds ggiivaiehs


541
srpinvikfs tdegqcwqty tftrdpiyft glasepgars mnisiwgfte sfltsqwvsy


601
tidfkdiler nceekdytiw lahstdpedy edgcilgyke qflrlrkssv cqngrdyvvt


661
kqpsicicsl edflcdfgyy rpendskcve qpelkghdle fclygreehl ttngyrkipg


721
dkcqggvnpv revkdlkkkc tsnflspekq nsksnsvpii laivglmlvt vvagvlivkk


781
yvcggrflvh rysvlqqhae angvdgvdal dtashtnksg yhddsdedll e


 







Protein sequence isoform 2 (SEQ ID NO (18)):








1
mtfgqsklyr sedygknfkd itdlinntfi rtefgmaigp ensgkvvlta evsggsrggr


61
ifrssdfakn fvqtdlpfhp ltqmmyspqn sdyllalste nglwvsknfg gkweeihkav


121
clakwgsdnt iffttyangs ctdlgalelw rtsdlgksfk tigvkiysfg lggrflfasv


181
madkdttrri hvstdqgdtw smaqlpsvgq eqfysilaan ddmvfmhvde pgdtgfgtif


241
tsddrgivys ksldrhlytt tggetdftnv tslrgvyits vlsednsiqt mitfdqggrw


301
thlrkpense cdataknkne cslhihasys isqklnvpma plsepnavgi viahgsvgda


361
isvmvpdvyi sddggyswtk mlegphyyti ldsggiivai ehssrpinvi kfstdegqcw


421
qtytftrdpi yftglasepg arsmnisiwg ftesfltsqw vsytidfkdi lernceekdy


481
tiwlahstdp edyedgcilg ykeqflrlrk ssvcqngrdy vvtkqpsicl csledflcdf


541
gyyrpendsk cveqpelkgh dlefclygre ehlttngyrk ipgdkcqggv npvrevkdlk


601
kkctsnflsp ekqnsksnsv piilaivglm lvtvvagvli vkkyvcggrf lvhrysvlqq


661
haeangvdgv daldtashtn ksgyhddsde dlle


 


Gene ID:
X11


Gene symbol:
ATP1B1


Gene description:
sodium/potassium-transporting ATPase subunit beta-1


Unigene:
Hs.291196


Genbank:
U16799


Entrez Gene:
481


Refseq:
NM__001677







Protein sequence (SEQ ID NO (19)):








1
margkakeeg swkkfiwnse kkeflgrtgg swfkillfyv ifygclagif igtiqvmllt


61
isefkptyqd rvappgltqi pqiqkteisf rpndpksyea yvlnivrfle kykdsaqrdd


121
mifedcgdvp sepkergdfn hergerkvcr fklewlgncs glndetygyk egkpciiikl


181
nrvlgfkpkp pknesletyp vmkynpnvlp vqctgkrded kdkvgnveyf glgnspgfpl


241
qyypyygkll qpkylqplla vqftnltmdt eirieckayg enigysekdr fqgrfdvkie


301
vks


 


Gene ID:
X12


Gene symbol:
AGRN


Gene description:
Agrin


Unigene:
Hs.273330


Genbank:
AB191264


Entrez Gene:
375790


Refseq:
NM_198576







Protein sequence (SEQ ID NO (20)):








1
magrshpgpl rpllpllvva acvlpgaggt cperalerre eeanvvltgt veeilnvdpv


61
qhtysckvrv wrylkgkdlv areslldggn kvvisgfgdp licdnqvstg dtriffvnpa


121
ppylwpahkn elmlnsslmr itlrnleeve fcvedkpgth ftpvpptppd acrgmlcgfg


181
avcepnaegp grascvckks pcpsvvapvc gsdastysne celqraqcsq qrrirllsrg


241
pcgsrdpcsn vtcsfgstca rsadgltasc lcpatcrgap egtvcgsdga dypgecqllr


301
racarqenvf kkfdgpcdpc qgalpdpsrs crvnprtrrp emllrpescp arqapvcgdd


361
gvtyendcvm grsgaargll lqkvrsgqcq grdqcpeper fnavclsrrg rprcscdrvt


421
cdgayrpvca qdgrtydsdc wrqqaecrqq raipskhqgp cdqapspclg vqcafgatca


481
vkngqaacec lqacsslydp vcgsdgvtyg saceleatac tlgreiqvar kgpcdrcgqc


541
rfgalceaet grcvcpsecv alaqpvcgsd ghtypsecml hvhacthqis lhvasagpce


601
tcgdavcafg avcsagqcvc prcehpppgp vcgsdgvtyg sacelreaac lqqtqieear


661
agpceqaecg sggsgsgedg dceqelcrqr ggiwdedsed gpcvcdfscq svpgspvcgs


721
dgvtystece lkkarcesqr glyvaaqgac rgptfaplpp vaplhcaqtp ygccqdnita


781
argvglagcp sacqcnphgs yggtcdpatg qcscrpgvgg lrcdrcepgf wnfrgivtdg


841
rsgctpcscd pqgavrddce qmtglcsckp gvagpkcgqc pdgralgpag ceadasapat


901
caemrcefga rcveesgsah cvcpmltcpe anatkvcgsd gvtygnecql ktiacrqglq


961
isiqslgpcq eavapsthpt sasvtvttpg lllsqalpap pgalplapss tahsqttppp


1021
ssrprttasv prttvwpvlt vpptapspap slvasafges gstdgssdee lsgdqeasgg


1081
gsgglepleg ssvatpgppv erascynsal gccsdgktps ldaegsncpa tkvfqgvlel


1141
egvegqelfy tpemadpkse lfgetarsie stlddlfrns dvkkdfrsvr lrdlgpgksv


1201
raivdvhfdp ttafrapdva rallrqiqvs rrrslgvrrp lqehvrfmdf dwfpafitga


1261
tsgaiaagat arattasrlp ssavtpraph pshtsqpvak ttaapttrrp pttapsrvpg


1321
rrppapqqpp kpcdsqpcfh ggtcqdwalg ggftcscpag rggavcekvl gapvpafegr


1381
sflafptlra yhtlrlalef ralepqglll yngnargkdf lalalldgrv qlrfdtgsgp


1441
avltsavpve pgqwhrlels rhwrrgtlsv dgetpvlges psgtdglnld tdlfvggvpe


1501
dqaavalert fvgaglrgci rlldvnnqrl elgigpgaat rgsgvgecgd hpclpnpchg


1561
gapcqnleag rfhcqcppgr vgptcadeks pcqpnpchga apcrvlpegg aqcecplgre


1621
gtfcqtasgq dgsgpfladf ngfshlelrg lhtfardlge kmalevvfla rgpsglllyn


1681
gqktdgkgdf vslalrdrrl efrydlgkga avirsrepvt lgawtrvsle rngrkgalrv


1741
gdgprvlges pvphtvlnlk eplyvggapd fsklaraaav ssgfdgaiql vslggrqllt


1801
pehvlrqvdv tsfaghpctr asghpclnga scvpreaayv clcpggfsgp hcekglveks


1861
agdvdtlafd grtfveylna vtesekalqs nhfelslrte atqglvlwsg kateradyva


1921
laivdghlql synlgsqpvv lrstvpvntn rwlrvvahre qregslqvgn eapvtgsspl


1981
gatqldtdga lwlgglpelp vgpalpkayg tgfvgclrdv vvgrhplhll edavtkpelr


2041
pcptp


 


Gene ID:
X13


Gene symbol:
APP


Gene description:
Amyloid beta A4 protein


Unigene:
Hs.434980


Genbank:




BC065529 | AF282245 | AK298861 | AK294534 | AK295621 | AK296229 | AK297412 |



AK297229 | AK295373 | BC004369 | M16765 | AK311717


Entrez Gene:
351


Refseq:
NM_000484 | NM_201413 | NM_001136130 | NM_201414 | NM_001136129







Protein sequence isoform a (SEQ ID NO (21)):








1
mlpglallll aawtaralev ptdgnaglla epqiamfcgr lnmhmnvqng kwdsdpsgtk


61
tcidtkegil qycqevypel qitnvveanq pvtiqnwckr grkqckthph fvipyrclvg


121
efvsdallvp dkckflhqer mdvcethlhw htvaketcse kstnlhdygm llpcgidkfr


181
gvefvccpla eesdnvdsad aeeddsdvww ggadtdyadg sedkvvevae eeevaeveee


241
eadddedded gdeveeeaee pyeeatertt siattttttt esveevvrev cseqaetgpc


301
ramisrwyfd vtegkcapff yggcggnrnn fdteeycmav cgsamsqsll kttqeplard


361
pvklpttaas tpdavdkyle tpgdenehah fqkakerlea khrermsqvm reweeaerqa


421
knlpkadkka viqhfqekve sleqeaaner qqlvethmar veamlndrrr lalenyital


481
qavpprprhv fnmlkkyvra eqkdrqhtlk hfehvrmvdp kkaaqirsqv mthlrviyer


541
mnqslsllyn vpavaeeiqd evdellqkeq nysddvlanm iseprisygn dalmpsltet


601
kttvellpvn gefslddlqp whsfgadsvp antenevepv darpaadrgl ttrpgsgltn


661
ikteeisevk mdaefrhdsg yevhhqklvf faedvgsnkg aiiglmvggv viatvivitl


721
vmlkkkqyts ihhgvvevda avtpeerhls kmqqngyenp tykffeqmqn


 







Protein sequence isoform b (SEQ ID NO (22)):








1
mlpglallll aawtaralev ptdgnaglla epqiamfcgr lnmhmnvqng kwdsdpsgtk


61
tcidtkegil qycqevypel qitnvveanq pvtiqnwckr grkqckthph fvipyrclvg


121
efvsdallvp dkckflhqer mdvcethlhw htvaketcse kstnlhdygm llpcgidkfr


181
gvefvccpla eesdnvdsad aeeddsdvww ggadtdyadg sedkvvevae eeevaeveee


241
eadddedded gdeveeeaee pyeeatertt siattttttt esveevvrev cseqaetgpc


301
ramisrwyfd vtegkcapff yggcggnrnn fdteeycmav cgsaipttaa stpdavdkyl


361
etpgdeneha hfqkakerle akhrermsqv mreweeaerq aknlpkadkk aviqhfqekv


421
esleqeaane rqqlvethma rveamlndrr rlalenyita lqavpprprh vfnmlkkyvr


481
aeqkdrqhtl khfehvrmvd pkkaaqirsq vmthlrviye rmnqslslly nvpavaeeiq


541
devdellqke qnysddvlan miseprisyg ndalmpslte tkttvellpv ngefslddlq


601
pwhsfgadsv pantenevep vdarpaadrg lttrpgsglt nikteeisev kmdaefrhds


661
gyevhhqklv ffaedvgsnk gaiiglmvgg vviatvivit lvmlkkkqyt sihhgvvevd


721
aavtpeerhl skmqqngyen ptykffeqmq n


 







Protein sequence isoform f (SEQ ID NO(23)):








1
mlpglallll aawtaralev ypelqitnvv eanqpvtiqn wckrgrkqck thphfvipyr


61
clvgefvsda llvpdkckfl hqermdvcet hlhwhtvake tcsekstnlh dygmllpcgi


121
dkfrgvefvc cplaeesdnv dsadaeedds dvwwggadtd yadgsedkvv evaeeeevae


181
veeeeaddde ddedgdevee eaeepyeeat erttsiattt ttttesveev vrevcseqae


241
tgperamisr wyfdvtegkc apffyggcgg nrnnfdteey cmavcgsams qsllkttqep


301
lardpvklpt taastpdavd kyletpgden ehahfqkake rleakhrerm sqvmreweea


361
erqaknlpka dkkaviqhfq ekvesleqea anerqqlvet hmarveamln drrrlaleny


421
italqavppr prhvfnmlkk yvraeqkdrq htlkhfehvr mvdpkkaaqi rsqvmthlrv


481
iyermnqsls llynvpavae eiqdevdell qkeqnysddv lanmisepri sygndalmps


541
ltetkttvel lpvngefsld dlqpwhsfga dsvpantene vepvdarpaa drglttrpgs


601
gltnikteei sevkmdaefr hdsgyevhhq klvffaedvg snkgaiiglm vggvviatvi


661
vitlvmlkkk qytsihhgvv evdaavtpee rhlskmqqng yenptykffe qmqn


 







Protein sequence isoform c (SEQ ID NO (24)):








1
mlpglallll aawtaralev ptdgnaglla epqiamfcgr lnmhmnvqng kwdsdpsgtk


61
tcidtkegil qycqevypel qitnvveanq pvtiqnwckr grkqckthph fvipyrclvg


121
efvsdallvp dkckflhqer mdvcethlhw htvaketcse kstnlhdygm llpcgidkfr


181
gvefvccpla eesdnvdsad aeeddsdvww ggadtdyadg sedkvvevae eeevaeveee


241
eadddedded gdeveeeaee pyeeatertt siattttttt esveevvrvp ttaastpdav


301
dkyletpgde nehahfqkak erleakhrer msqvmrewee aerqaknlpk adkkaviqhf


361
qekvesleqe aanerqqlve thmarveaml ndrrrlalen yitalqavpp rprhvfnmlk


421
kyvraeqkdr qhtlkhfehv rmvdpkkaaq irsqvmthlr viyermnqsl sllynvpava


481
eeiqdevdel lqkeqnysdd vlanmisepr isygndalmp sltetkttve llpvngefsl


541
ddlqpwhsfg adsvpanten evepvdarpa adrglttrpg sgltniktee isevkmdaef


601
rhdsgyevhh qklvffaedv gsnkgaiigl mvggvviatv ivitivmlkk kqytsihhgv


661
vevdaavtpe erhlskmqqn gyenptykff eqmqn


 







Protein sequence isoform e (SEQ ID NO (25)):








1
mlpglallll aawtaralev ypelqitnvv eanqpvtiqn wckrgrkqck thphfvipyr


61
clvgefvsda llvpdkckfl hqermdvcet hlhwhtvake tcsekstnlh dygmllpcgi


121
dkfrgvefvc cplaeesdnv dsadaeedds dvwwggadtd yadgsedkvv evaeeeevae


181
veeeeaddde ddedgdevee eaeepyeeat erttsiattt ttttesveev vrvpttaast


241
pdavdkylet pgdenehahf qkakerleak hrermsqvmr eweeaerqak nlpkadkkav


301
iqhfqekves leqeaanerq qlvethmarv eamlndrrrl alenyitalq avpprprhvf


361
nmlkkyvrae qkdrqhtlkh fehvrmvdpk kaaqirsqvm thlrviyerm nqslsllynv


421
pavaeeiqde vdellqkeqn ysddvlanmi seprisygnd almpsltetk ttvellpvng


481
efslddlqpw hsfgadsvpa ntenevepvd arpaadrglt trpgsgltni kteeisevkm


541
daefrhdsgy evhhqklvff aedvgsnkga iiglmvggvv iatvivitlv mlkkkqytsi


601
hhgvvevdaa vtpeerhlsk mqqngyenpt ykffeqmqn


 


Gene ID:
X14


Gene symbol:
COLEC12


Gene description:
Collectin sub-family member 12


Unigene:
Hs.464422


Genbank:
AB038518


Entrez Gene:
81035


Refseq:
NM_130386







Protein sequence (SEQ ID NO (26)):








1
mkddfaeeee vqsfgykrfg iqegtqctkc knnwalkfsi illyilcall titvailgyk


61
vvekmdnvtg gmetsrqtyd dkltavesdl kklgdqtgkk aistnselst frsdildlrq


121
qlreitekts knkdtleklq asgdalvdrq sqlketlenn sflittvnkt lqayngyvtn


181
lqqdtsvlqg nlqnqmyshn vvimnlnnln ltqvqqrnli tnlqrsvddt sqaiqriknd


241
fqnlqqvflq akkdtdwlke kvqslqtlaa nnsalakann dtledmnsql nsftgqmeni


301
ttisqaneqn lkdlqdlhkd aenrtaikfn qleerfqlfe tdivniisni sytahhlrtl


361
tsnlnevrtt ctdtltkhtd dltslnntla nirldsyslr mqqdlmrsrl dtevanlsvi


421
meemklvdsk hgqliknfti lqgppgprgp rgdrgsqgpp gptgnkgqkg ekgepgppgp


481
agergpigpa gppgerggkg skgsqgpkgs rgspgkpgpq gpsgdpgppg ppgkeglpgp


541
qgppgfqglq gtvgepgvpg prglpglpgv pgmpgpkgpp gppgpsgavv plalqneptp


601
apedngcpph wknftdkcyy fsvekeifed aklfcedkss hlvfintree qqwikkqmvg


661
reshwigltd serenewkwl dgtspdyknw kagqpdnwgh ghgpgedcag liyagqwndf


721
qcedvnnfic ekdretvlss al


 


Gene ID:
X15


Gene symbol:
NCAM1


Gene description:
Neural cell adhesion molecule 1


Unigene:
Hs.503878


Genbank:
BC047244


Entrez Gene:
4684


Refseq:
NM_000615 | NM_001076682 | NM_181351 | NM_001242608 l



NM_001242607







Protein sequence isoform 1 (SEQ ID NO (27)):








1
mlqtkdliwt lfflgtavsl qvdivpsqge isvgeskffl cqvagdakdk diswfspnge


61
kltpnqqris vvwnddssst ltiynanidd agiykcvvtg edgseseatv nvkifqklmf


121
knaptpqefr egedavivcd vvsslpptii wkhkgrdvil kkdvrfivls nnylqirgik


181
ktdegtyrce grilargein fkdiqvivnv pptiqarqni vnatanlgqs vtlvcdaegf


241
peptmswtkd geqieqeedd ekyifsddss qltikkvdkn deaeyiciae nkageqdati


301
hlkvfakpki tyvenqtame leeqvtltce asgdpipsit wrtstrniss eektldghmv


361
vrsharvssl tlksiqytda geyictasnt igqdsqsmyl evqyapklqg pvavytwegn


421
qvnitcevfa ypsatiswfr dgqllpssny snikiyntps asylevtpds endfgnynct


481
avnrigqesl efilvqadtp sspsidqvep ysstaqvqfd epeatggvpi lkykaewrav


541
geevwhskwy dakeasmegi vtivglkpet tyavrlaaln gkglgeisaa sefktqpvqg


601
epsapklegq mgedgnsikv nlikqddggs pirhylvryr alssewkpei rlpsgsdhvm


661
lksldwnaey evyvvaenqq gkskaahfvf rtsaqptaip angsptsgls tgaivgiliv


721
ifvlllvvvd itcyflnkcg lfmciavnlc gkagpgakgk dmeegkaafs kdeskepive


781
vrteeertpn hdggkhtepn ettpltepek gpveakpecq etetkpapae vktvpndatq


841
tkeneska


 







Protein sequence isoform 3 (SEQ ID NO (28)):








1
mlqtkdliwt lfflgtavsl qvdivpsqge isvgeskffl cqvagdakdk diswfspnge


61
kltpnqqris vvwnddssst ltiynanidd agiykcvvtg edgseseatv nvkifqklmf


121
knaptpqefr egedavivcd vvsslpptii wkhkgrdvil kkdvrfivls nnylqirgik


181
ktdegtyrce grilargein fkdiqvivnv pptiqarqni vnatanlgqs vtlvcdaegf


241
peptmswtkd geqieqeedd ekyifsddss qltikkvdkn deaeyiciae nkageqdati


301
hlkvfakpki tyvenqtame leeqvtltce asgdpipsit wrtstrniss eektldghmv


361
vrsharvssl tlksiqytda geyictasnt igqdsqsmyl evqyapklqg pvavytwegn


421
qvnitcevfa ypsatiswfr dgqllpssny snikiyntps asylevtpds endfgnynct


481
avnrigqesl efilvqadtp sspsidqvep ysstaqvqfd epeatggvpi lkykaewrav


541
geevwhskwy dakeasmegi vtivglkpet tyavrlaaln gkglgeisaa sefktqpvhs


601
ppppasasss tpvplsppdt twplpalatt epakgepsap klegqmgedg nsikvnlikq


661
ddggspirhy lvryralsse wkpeirlpsg sdhvmlksld wnaeyevyvv aenqqgkska


721
ahfvfrtsaq ptaipatlgg nsasyffvsl lfsavtllll c


 







Protein sequence isoform 2 (SEQ ID NO (29)):








1
mlqtkdliwt lfflgtavsl qvdivpsqge isvgeskffl cqvagdakdk diswfspnge


61
kltpnqqris vvwnddssst ltiynanidd agiykcvvtg edgseseatv nvkifqklmf


121
knaptpqefr egedavivcd vvsslpptii wkhkgrdvil kkdvrfivls nnylqirgik


181
ktdegtyrce grilargein fkdiqvivnv pptiqarqni vnatanlgqs vtivcdaegf


241
peptmswtkd geqieqeedd ekyifsddss qltikkvdkn deaeyiciae nkageqdati


301
hlkvfakpki tyvenqtame leeqvtltce asgdpipsit wrtstrniss eekaswtrpe


361
kqetldghmv vrsharvssl tlksiqytda geyictasnt igqdsqsmyl evqyapklqg


421
pvavytwegn qvnitcevfa ypsatiswfr dgqllpssny snikiyntps asylevtpds


481
endfgnynct avnrigqesl efilvqadtp sspsidqvep ysstaqvqfd epeatggvpi


541
lkykaewrav geevwhskwy dakeasmegi vtivglkpet tyavrlaaln gkglgeisaa


601
sefktqpvqg epsapklegq mgedgnsikv nlikqddggs pirhylvryr alssewkpei


661
rlpsgsdhvm lksldwnaey evyvvaenqq gkskaahfvf rtsaqptaip angsptsgls


721
tgaivgiliv ifvlllvvvd itcyflnkcg lfmciavnlc gkagpgakgk dmeegkaafs


781
kdeskepive vrteeertpn hdggkhtepn ettpltepek gpveakpecq etetkpapae


841
vktvpndatq tkeneska


 







Protein sequence isoform 4 (SEQ ID NO(30)):








1
mlqtkdliwt lfflgtavsl qvdivpsqge isvgeskffl cqvagdakdk diswfspnge


61
kltpnqqris vvwnddssst ltiynanidd agiykcvvtg edgseseatv nvkifqklmf


121
knaptpqefr egedavivcd vvsslpptii wkhkgrdvil kkdvrfivls nnylqirgik


181
ktdegtyrce grilargein fkdiqvivnv pptiqarqni vnatanlgqs vtlvcdaegf


241
peptmswtkd geqieqeedd ekyifsddss qltikkvdkn deaeyiciae nkageqdati


301
hlkvfakpki tyvenqtame leeqvtltce asgdpipsit wrtstrniss eektldghmv


361
vrsharvssl tlksiqytda geyictasnt igqdsqsmyl evqyapklqg pvavytwegn


421
qvnitcevfa ypsatiswfr dgqllpssny snikiyntps asylevtpds endfgnynct


481
avnrigqesl efilvqadtp sspsidqvep ysstaqvqfd epeatggvpi lkykaewrav


541
geevwhskwy dakeasmegi vtivglkpet tyavrlaaln gkglgeisaa sefktqpvqg


601
epsapklegq mgedgnsikv nlikqddggs pirhylvryr alssewkpei rlpsgsdhvm


661
lksldwnaey evyvvaenqq gkskaahfvf rtsaqptaip atlggnsasy tfvsllfsav


721
tllllc


 







Protein sequence isoform 5 (SEQ ID NO (31)):








1
mlqtkdliwt lfflgtavsl qvdivpsqge isvgeskffl cqvagdakdk diswfspnge


61
kltpnqqris vvwnddssst ltiynanidd agiykcvvtg edgseseatv nvkifqklmf


121
knaptpqefr egedavivcd vvsslpptii wkhkgrdvil kkdvrfivls nnylqirgik


181
ktdegtyrce grilargein fkdiqvivnv pptiqarqni vnatanlgqs vtlvcdaegf


241
peptmswtkd geqieqeedd ekyifsddss qltikkvdkn deaeyiciae nkageqdati


301
hlkvfakpki tyvenqtame leeqvtltce asgdpipsit wrtstrniss eekaswtrpe


361
kqevhapwnw qvgrqkgqag sagfpgshet ldghmvvrsh arvssltlks iqytdageyi


421
ctasntigqd sqsmylevqy apklqgpvav ytwegnqvni tcevfaypsa tiswfrdgql


481
lpssnysnik iyntpsasyl evtpdsendf gnynctavnr igqeslefil vqadtpssps


541
idqvepysst aqvqfdepea tggvpilkyk aewravgeev whskwydake asmegivtiv


601
glkpettyav rlaalngkgl geisaasefk tqpvqgepsa pklegqmged gnsikvnlik


661
qddggspirh ylvryralss ewkpeirlps gsdhvmlksl dwnaeyevyv vaenqqgksk


721
aahfvfrtsa qptaipangs ptsglstgai vgilivifvl llvvvditcy flnkcglfmc


781
iavnlcgkag pgakgkdmee gkaafskdes kepivevrte eertpnhdgg khtepnettp


841
ltepekgpve akpecqetet kpapaevktv pndatqtken eska


 


Gene ID:
X16


Gene symbol:
NRP2


Gene description:
Neuropilin-2


Unigene:
Hs.471200


Genbank:
BX537423 | AF016098 | BC101525 | BC104770 |BC117413 | BC143238 | BC143608 |



AF022860 | AF280545 | AF280544 | AF022859 | AK290934 | AF280546 |



BC009222 | AL833606 | BX648292 | AK130198 | BC018631


Entrez Gene:
8828


Refseq:




NM_201266 | NM_003872 | NM_201279 | NM_0185341NM_201267 | NM_201264







Protein sequence isoform 1 (SEQ ID NO (32)):








1
mdmfpltwvf lalyfsrhqv rgqpdppcgg rlnskdagyi tspgypqdyp shqncewivy


61
apepnqkivl nfnphfeiek hdckydfiei rdgdsesadl lgkhcgniap ptiissgsml


121
yikftsdyar qgagfslrye ifktgsedcs knftspngti espgfpekyp hnldctftil


181
akpkmeiilq flifdlehdp lqvgegdcky dwldiwdgip hvgpligkyc gtktpselrs


241
stgilsltfh tdmavakdgf saryylvhqe plenfqcnvp lgmesgrian eqisasstys


301
dgrwtpqqsr lhgddngwtp nldsnkeylq vdlrfltmlt aiatqgaisr etqngyyvks


361
yklevstnge dwmvyrhgkn hkvfqannda tevvlnklha plltrfvrir pqtwhsgial


421
rlelfgcrvt dapcsnmlgm lsgliadsqi sasstqeylw spsaarlvss rsgwfpripq


481
aqpgeewlqv dlgtpktvkg viiqgarggd sitavearaf vrkfkvsysl ngkdweyiqd


541
prtqqpklfe gnmhydtpdi rrfdpipaqy vrvyperwsp agigmrlevl gcdwtdskpt


601
vetlgptvks eetttpypte eeatecgenc sfeddkdlql psgfncnfdf leepcgwmyd


661
hakwlrttwa sssspndrtf pddrnflrlq sdsqregqya rlisppvhlp rspvcmefqy


721
qatggrgval qvvreasqes kllwviredq ggewkhgrii lpsydmeyqi vfegvigkgr


781
sgeiaiddir litdvplenc mepisafage nfkvdipeih eregyedeid deyevdwsns


841
ssatsgsgap stdkekswly tldpilitii amsslgvllg atcaglllyc tcsysglssr


901
scttlenynf elydglkhkv kmnhqkccse a


 







Protein sequence isoform 2 (SEQ ID NO (33)):








1
mdmfpltwvf lalyfsrhqv rgqpdppcgg rlnskdagyi tspgypqdyp shqncewivy


61
apepnqkivl nfnphfeiek hdckydfiei rdgdsesadl lgkhcgniap ptiissgsml


121
yikftsdyar qgagfslrye ifktgsedcs knftspngti espgfpekyp hnldctftil


181
akpkmeiilq flifdlehdp lqvgegdcky dwldiwdgip hvgpligkyc gtktpselrs


241
stgilsltfh tdmavakdgf saryylvhqe plenfqcnvp lgmesgrian eqisasstys


301
dgrwtpqqsr lhgddngwtp nldsnkeylq vdlrfltmlt aiatqgaisr etqngyyvks


361
yklevstnge dwmvyrhgkn hkvfqannda tevvlnklha plltrfvrir pqtwhsgial


421
rlelfgcrvt dapcsnmlgm lsgliadsqi sasstqeylw spsaarlvss rsgwfpripq


481
aqpgeewlqv dlgtpktvkg viiqgarggd sitavearaf vrkfkvsysl ngkdweyiqd


541
prtqqpklfe gnmhydtpdi rrfdpipaqy vrvyperwsp agigmrlevl gcdwtdskpt


601
vetlgptvks eetttpypte eeatecgenc sfeddkdlql psgfncnfdf leepcgwmyd


661
hakwlrttwa sssspndrtf pddrnflrlq sdsqregqya rlisppvhlp rspvcmefqy


721
qatggrgval qvvreasqes kllwviredq ggewkhgrii lpsydmeyqi vfegvigkgr


781
sgeiaiddir istdvplenc mepisafavd ipeiheregy edeiddeyev dwsnsssats


841
gsgapstdke kswlytldpi litiiamssl gvllgatcag lllyctcsys glssrscttl


901
enynfelydg lkhkvkmnhq kccsea


 







Protein sequence isoform 3 (SEQ ID NO (34)):








1
mdmfpltwvf lalyfsrhqv rgqpdppcgg rlnskdagyi tspgypqdyp shqncewivy


61
apepnqkivl nfnphfeiek hdckydfiei rdgdsesadl lgkhcgniap ptiissgsml


121
yikftsdyar qgagfslrye ifktgsedcs knftspngti espgfpekyp hnldctftil


181
akpkmeiilq flifdlehdp lqvgegdcky dwldiwdgip hvgpligkyc gtktpselrs


241
stgilsltfh tdmavakdgf saryylvhqe plenfqcnvp lgmesgrian eqisasstys


301
dgrwtpqqsr lhgddngwtp nldsnkeylq vdlrfltmlt aiatqgaisr etqngyyvks


361
yklevstnge dwmvyrhgkn hkvfqannda tevvlnklha plltrfvrir pqtwhsgial


421
rlelfgcrvt dapcsnmlgm lsgliadsqi sasstqeylw spsaarlvss rsgwfpripq


481
aqpgeewlqv dlgtpktvkg viiqgarggd sitavearaf vrkfkvsysl ngkdweyiqd


541
prtqqpklfe gnmhydtpdi rrfdpipaqy vrvyperwsp agigmrlevl gcdwtdskpt


601
vetlgptvks eetttpypte eeatecgenc sfeddkdlql psgfncnfdf leepcgwmyd


661
hakwlrttwa sssspndrtf pddrnflrlq sdsqregqya rlisppvhlp rspvcmefqy


721
qatggrgval qvvreasqes kllwviredq ggewkhgrii lpsydmeyqi vfegvigkgr


781
sgeiaiddir istdvplenc mepisafade yevdwsnsss atsgsgapst dkekswlytl


841
dpilitiiam sslgvllgat caglllyctc sysglssrsc ttlenynfel ydglkhkvkm


901
nhqkccsea


 







Protein sequence isoform 4 (SEQ ID NO (35)):








1
mdmfpltwvf lalyfsrhqv rgqpdppcgg rlnskdagyi tspgypqdyp shqncewivy


61
apepnqkivl nfnphfeiek hdckydfiei rdgdsesadl lgkhcgniap ptiissgsml


121
yikftsdyar qgagfslrye ifktgsedcs knftspngti espgfpekyp hnldctftil


181
akpkmeiilq flifdlehdp lqvgegdcky dwldiwdgip hvgpligkyc gtktpselrs


241
stgilsltfh tdmavakdgf saryylvhqe plenfqcnvp lgmesgrian eqisasstys


301
dgrwtpqqsr lhgddngwtp nldsnkeylq vdlrfltmlt aiatqgaisr etqngyyvks


361
yklevstnge dwmvyrhgkn hkvfqannda tevvlnklha plltrfvrir pqtwhsgial


421
rlelfgcrvt dapcsnmlgm lsgliadsqi sasstqeylw spsaarlvss rsgwfpripq


481
aqpgeewlqv dlgtpktvkg viiqgarggd sitavearaf vrkfkvsysl ngkdweyiqd


541
prtqqpklfe gnmhydtpdi rrfdpipaqy vrvyperwsp agigmrlevl gcdwtdskpt


601
vetlgptvks eetttpypte eeatecgenc sfeddkdlql psgfncnfdf leepcgwmyd


661
hakwlrttwa sssspndrtf pddrnflrlq sdsqregqya rlisppvhlp rspvcmefqy


721
qatggrgval qvvreasqes kllwviredq ggewkhgrii lpsydmeyqi vfegvigkgr


781
sgeiaiddir istdvplenc mepisafage nfkggtllpg teptvdtvpm qpipaywyyv


841
maaggavlvl vsvalalvlh yhrfryaakk tdhsitykts hytngaplav eptltikleq


901
drgshc


 







Protein sequence isoform 5 (SEQ ID NO (36)):








1
mdmfpltwvf lalyfsrhqv rgqpdppcgg rlnskdagyi tspgypqdyp shqncewivy


61
apepnqkivl nfnphfeiek hdckydfiei rdgdsesadl lgkhcgniap ptiissgsml


121
yikftsdyar qgagfslrye ifktgsedcs knftspngti espgfpekyp hnldctftil


181
akpkmeiilq flifdlehdp lqvgegdcky dwldiwdgip hvgpligkyc gtktpselrs


241
stgilsltfh tdmavakdgf saryylvhqe plenfqcnvp lgmesgrian eqisasstys


301
dgrwtpqqsr lhgddngwtp nldsnkeylq vdlrfltmlt aiatqgaisr etqngyyvks


361
yklevstnge dwmvyrhgkn hkvfqannda tevvlnklha plltrfvrir pqtwhsgial


421
rlelfgcrvt dapcsnmlgm lsgliadsqi sasstqeylw spsaarlvss rsgwfpripq


481
aqpgeewlqv dlgtpktvkg viiqgarggd sitavearaf vrkfkvsysl ngkdweyiqd


541
prtqqpklfe gnmhydtpdi rrfdpipaqy vrvyperwsp agigmrlevl gcdwtdskpt


601
vetlgptvks eetttpypte eeatecgenc sfeddkdlql psgfncnfdf leepcgwmyd


661
hakwlrttwa sssspndrtf pddrnflrlq sdsqregqya rlisppvhlp rspvcmefqy


721
qatggrgval qvvreasqes kllwviredq ggewkhgrii lpsydmeyqi vfegvigkgr


781
sgeiaiddir istdvplenc mepisafagg tllpgteptv dtvpmqpipa ywyyvmaagg


841
avlvlvsval alvlhyhrfr yaakktdhsi tyktshytng aplaveptlt ikleqdrgsh


901
c


 







Protein sequence isoform 6 (SEQ ID NO (37)):








1
mdmfpltwvf lalyfsrhqv rgqpdppcgg rlnskdagyi tspgypqdyp shqncewivy


61
apepnqkivl nfnphfeiek hdckydfiei rdgdsesadl lgkhcgniap ptiissgsml


121
yikftsdyar qgagfslrye ifktgsedcs knftspngti espgfpekyp hnldctftil


181
akpkmeiilq flifdlehdp lqvgegdcky dwldiwdgip hvgpligkyc gtktpselrs


241
stgilsltfh tdmavakdgf saryylvhqe plenfqcnvp lgmesgrian eqisasstys


301
dgrwtpqqsr lhgddngwtp nldsnkeylq vdlrfltmlt aiatqgaisr etqngyyvks


361
yklevstnge dwmvyrhgkn hkvfqannda tevvlnklha plltrfvrir pqtwhsgial


421
rlelfgcrvt dapcsnmlgm lsgliadsqi sasstqeylw spsaarlvss rsgwfpripq


481
aqpgeewlqv dlgtpktvkg viiqgarggd sitavearaf vrkfkvsysl ngkdweyiqd


541
prtqqpkvgc swrpl


 


Gene ID:
X17


Gene symbol:
PLXNA2


Gene description:
Plexin-A2


Unigene:
Hs.497626


Genbank:
BC132676


Entrez Gene:
5362


Refseq:
NM__025179







Protein sequence (SEQ ID NO (38)):








1
meqrrpwpra levdsrsvvl lsvvwvllap paagmpqfst fhsenrdwtf nhltvhqgtg


61
avyvgainrv ykltgnltiq vahktgpeed nkscyppliv qpcsevltlt nnvnklliid


121
ysenrllacg slyqgvckll rlddlfilve pshkkehyls svnktgtmyg vivrsegedg


181
klfigtavdg kqdyfptlss rklprdpess amldyelhsd fvsslikips dtlalvshfd


241
ifyiygfasg gfvyfltvqp etpegvains agdlfytsri vrlckddpkf hsyvslpfgc


301
tragveyrll qaaylakpgd slaqafnits qddvlfaifs kgqkqyhhpp ddsalcafpi


361
rainlqiker lqscyqgegn lelnwllgkd vqctkapvpi ddnfcgldin qplggstpve


421
gltlyttsrd rmtsvasyvy ngysvvfvgt ksgklkkira dgpphggvqy emvsylkdgs


481
pilrdmafsi dqrylyvmse rqvtrvpves ceqyttcgec lssgdphcgw calhnmcsrr


541
dkcqqawepn rfaasisqcv slavhpssis vsehsrllsl vvsdapdlsa giacafgnlt


601
evegqvsgsq vicispgpkd vpvipldqdw fglelqlrsk etgkifvste fkfyncsahq


661
lclscvnsaf rchwckyrnl cthdpttcsf qegrinised cpqlvpteei lipvgevkpi


721
tlkarnlpqp qsgqrgyecv lniqgaihrv palrfnsssv qcqnssyqyd gmdisnlavd


781
favvwngnfi idnpqdlkvh lykcaagres cglclkadrk fecgwcsger rctlhqhcts


841
psspwldwss hnvkcsnpqi teiltvsgpp eggtrvtihg vnlgldfsei ahhvqvagvp


901
ctplpgeyii aeqivcemgh alvgttsgpv rlcigeckpe fmtkshqqyt fvnpsvlsln


961
pirgpesggt mvtitghylg agssvavylg nqtcefygrs mseivcvspp ssnglgpvpv


1021
sysvdrahvd snlqfeyidd prvqriepew siasghtplt itgfnldviq eprirvkfng


1081
kesvnyckvv ntttltclap slttdyrpgl dtverpdefg fvfnnvqsll iyndtkfiyy


1141
pnptfellsp tgvldqkpgs piilkgknlc ppasggakln ytvligetpc avtvsetqll


1201
ceppnitgqh kvmvhvggmv fspgsysvis dslltlpaiv siaaggslll iiviivliay


1261
krksrendlt lkrlqmqmdn lesrvaleck eafaelqtdi neltsdldrs gipyldyrty


1321
amrvlfpgie dhpvlrelev qgngqqhvek alklfaqlin nkvflltfir tlelqrsfsm


1381
rdrgnvasli mtglqgrley atdvlkqlls dlidknlenk nhpklllrrt esvaekmltn


1441
wfafllhkfl kecageplfm lycaikqqme kgpidaitge aryslsedkl irqqieyktl


1501
ilncvnpdne nspeipvkvl ncdtitqvke kildavyknv pysqrpravd mdlewrqgri


1561
arvvlqdedi ttkiegdwkr lntlmhyqvs drsvvalvpk qtssynipas asisrtsisr


1621
ydssfrytgs pdslrsrapm itpdlesgvk vwhlvknhdh gdqkegdrgs kmvseiyltr


1681
llatkgtlqk fvddlfetlf stvhrgsalp laikymfdfl deqadrhsih dtdvrhtwks


1741
nclplrfwvn viknpqfvfd ihkgsitdac lsvvaqtfmd scstsehrlg kdspsnklly


1801
akdipsyksw veryyadiak lpaisdqdmn aylaeqsrlh avefnmlsal neiysyvsky


1861
seeligaleq deqarrqrla ykveqlinam sies


 


Gene ID:
X18


Gene symbol:
PCDHA4


Gene description:
Protocadherin alpha-4


Unigene:
Hs.199343


Genbank:
AF152482 | AF152312


Entrez Gene:
56144


Refseq:
NM_0189071 NM_031500







Protein sequence isoform 1 (SEQ ID NO (39)):








1
mefswgsgqe srrlllllll laaweagngq lhysyseeak hgtfvgriaq dlglelaelv


61
prlfrvaskg rggllevnlq ngilfvnsri dreelcrrsa ecsihleviv drplqvfhvd


121
vevrdindnp pvfpatqknl siaesrplds rfplegasda digenallty rlspneyfsl


181
ekppddelvk glglilrksl dreeapeifl vltatdggkp eltgtvqlli tvldandnap


241
afdrtiykvr llenvpngtl viklnasdld eglngdivys fsndispnvk skfhidpitg


301
qiivkgyidf eesksyeiiv egidkgqlpl sghcrvivev ednndnvpdl efkslslpir


361
edaplgtvia lisvsdkdmg vnglvtcslt shvpfklyst fknyyslvld saldresysa


421
yelvvtardg gspslwatas vsvevadvnd napafaqpey tvfvkennpp gchiftvsaw


481
dadaqenalv syslverrvg eralssyvsv haesgkvyal qpldheelel lqfqvtarda


541
gvpplgsnvt lqvfvldend napallapra ggtggavsel vpwsvgvghv vakvravdad


601
sgynawlsye lqpgtggari pfrvglytge isttraldet daprhrllvl vkdhgepalt


661
atatvlvslv esgqapkass ralvgavgpd aalvdvnvyl iiaicavssl lvltlllyta


721
lrcsalpteg acapgkptlv cssavgswsy sqqrrprvcs gegppktdlm afspslpdsr


781
dredqlqtte esfakprqpn pdwrysaslr agmhssvhle eagilragpg gpdqqwptvs


841
satpepeage vsppvgagvn snswtfkygp gnpkgsgpge lpdkfiipgs paiisirqep


901
tnsqidksdf itfgkkeetk kkkkkkkgnk tqekkekgns ttdnsdq


 







Protein sequence isoform 2 (SEQ ID NO (40)):








1
mefswgsgqe srrlllllll laaweagngq lhysvseeak hgtfvgriaq dlglelaelv


61
prlfrvaskg rggllevnlq ngilfvnsri dreelcrrsa ecsihleviv drplqvfhvd


121
vevrdindnp pvfpatqknl siaesrplds rfplegasda digenallty rlspneyfsl


181
ekppddelvk glglilrksl dreeapeifl vltatdggkp eltgtvqlli tvldandnap


241
afdrtiykvr llenvpngtl viklnasdld eglngdivys fsndispnvk skfhidpitg


301
qiivkgyidf eesksyeiiv egidkgqlpl sghcrvivev ednndnvpdl efkslslpir


361
edaplgtvia lisysdkdmg vnglvtcslt shvpfklvst fknyyslvld saldresysa


421
yelvvtardg gspslwatas vsvevadvnd napafaqpey tvfvkennpp gchiftvsaw


481
dadaqenalv syslverrvg eralssyvsv haesgkvyal qpldheelel lqfqvtarda


541
gvpplgsnvt lqvfvldend napallapra ggtggavsel vpwsvgvghv vakvravdad


601
sgynawlsye lqpgtggari pfrvglytge isttraldet daprhrllvl vkdhgepalt


661
atatvlvslv esgqapkass ralvgavgpd aalvdvnvyl iiaicavssl lvltlllyta


721
lrcsalpteg acapgkptlv cssavgswsy sqqrrprvcs gegppktdlm afspslpdsr


781
dredqlqtte esfakvsv


 


Gene ID:
X19


Gene symbol:
PCDHAC2


Gene description:
Protocadherin alpha-C2


Unigene:
Hs.199343


Genbank:
AF152304


Entrez Gene:
56134


Refseq:
NM_018899 | NM_031883







Protein sequence isoform 1 (SEQ ID NO (41)):








1
meqagtrpaa tehprlrrpm pwllllplll llllllpgpa asqlrysvpe eqapgalvgn


61
varalglelr rlgpgclrin hlgapspryl eldltsgalf vneridreal ceqrprells


121
levlahnpva vsaveveild indnsprfpr pnyqlqvses vapgarfhie saqdpdvgan


181
svqtyelsps ehfeldlkpl genskvlelv lrkgldreqa alhhlvltav dggiparsgt


241
aqisvrvldt ndnspafdqs tyrvqlreds ppgtlcckln asdpdegsng elryslssyt


301
sdrerqlfsi dastgevrvi ggldyeeass yqiyvqatdr gpvpmaghck vlvdivdvnd


361
napevvltdl yspvpenatp ntivavlsvn dqdsgpnrkv slgleatlpf rlngfgnsyt


421
lvvsgpldre rvavynitvt atdggipqlt slrtlkveis dindnppsfl edsysiyiqe


481
nnlpgvllct vqatdpdeke naevtyslle reiqglpvts yvsinsasgs lyavnsfdye


541
kfreffctve aqdkgsppls stvtanvyvv dmndhaphil yptstnssaa femvprtapa


601
gylvtkviam dsdsgqnawl fyhlaqtsdl dlfkvelhtg eirttrkmgd esgstfnitv


661
vvrdngepsl sasvaitvav vdrvskilpd tqrhyksprt yseitlylii alstvsfifl


721
ltiiilsiik cyrytaygta ccggfcgvre rspaelykqa nnnidariph glkvqphfie


781
vrgngsltkt ycykacltag sgsdtfmfyn tgaqtgpgps gaqaavtdsr nltgqsgqna


841
gnliilknea vsqneprqpn pdwrysaslr agmhssvhle eagilragpg gpdqqwptvs


901
satpepeage vsppvgagvn snswtfkygp gnpkgsgpge lpdkfiipgs paiisirqep


961
tnsqidksdf itfgkkeetk kkkkkkkgnk tqekkekgns ttdnsdq


 







Protein sequence isoform 2 (SEQ ID NO (42)):








1
meqagtrpaa tehprlrrpm pwllllplll llllllpgpa asqlrysvpe eqapgalvgn


61
varalglelr rlgpgclrin hlgapspryl eldltsgalf vneridreal ceqrprclls


121
levlahnpva vsaveveild indnsprfpr pnyqlqvses vapgarfhie saqdpdvgan


181
svqtyelsps ehfeldlkpl qenskvlelv lrkgldreqa alhhlvltav dggiparsgt


241
aqisvrvldt ndnspafdqs tyrvqlreds ppgtlvvkln asdpdegsng elryslssyt


301
sdrerqlfsi dastgevrvi ggldyeeass yqiyvqatdr gpvpmaghck vlvdivdvnd


361
napevvltdl yspvpenatp ntivavlsvn dqdsgpnrkv slgleatlpf rlngfgnsyt


421
lvvsgpldre rvavynitvt atdggipqlt slrtlkveis dindnppsfl edsysiyiqe


481
nnlpgvllct vqatdpdeke naevtyslle reiqglpvts yvsinsasgs lyavnsfdye


541
kfreffvtve aqdkgsppls stvtanvyvv dmndhaphil yptstnssaa femvprtapa


601
gylvtkviam dsdsgqnawl fyhlaqtsdl dlfkvelhtg eirttrkmgd esgstfnitv


661
vvrdngepsl sasvaitvav vdrvskilpd tqrhvksprt yseitlylii alstvsfifl


721
ltiiilsiik cyrytaygta ccggfcgvre rspaelykqa nnnidariph glkvqphfie


781
vrgngsltkt ycykacltag sgsdtfmfyn tgaqtgpgps gaqaavtdsr nitgqsgqna


841
gnliilknea vsqnevrqws ggllqthafv thppiscdla llsh


 


Gene ID:
X20


Gene symbol:
GPC4


Gene description:
Glypican 4


Unigene:
Hs.58367


Genbank:
AF030186


Entrez Gene:
2239


Refseq:
NM_001448







Protein sequence (SEQ ID NO (43)):








1
mrllwklvil lplinssagd gllsrpiftq ephdvifpld lsksevilnc aangypsphy


61
rwkqngtdid ftmsyhyrld ggslainsph tdqdigmyqc latnllgtil srkaklqfay


121
iedfetktrs tvsvregqgv vllcgppphf gdlsyawtfn dnplyvqedn rrfvsqetgn


181
lyiakvepsd vgnytcfitn keaqrsvqgp ptplvqrtdg vmgeyepkie vrfpetiqaa


241
kdssvklecf algnpvpdis wrrldgsplp gkvkysksqa ileipnfqqe degfyecias


301
nlrgrnlakg qlifyappew eqkiqnthls iydnllweck asgkpnpwyt wlkngerlnp


361
eeriqiengt liitmlnvsd sgvyqcaaen kyqiiyanae lrvlasapdf skspvkkksf


421
vqvggdivig ckpnafpraa iswkrgtetl rqskriflle dgslkiynit rsdagsytci


481
atnqfgtakn tgslivkert vitvppskmd vtvgesivlp cqvshdpsie vvfvwffngd


541
vidlkkgvah feriggesvg dlmirniqlh hsgkylctvq ttleslsava diivrgppgp


601
pedvqvedis sttsqlswra gpdnnspiqi ftiqtrtpfs vgwqavatvp eilngktyna


661
tvvglspwve yefrvvagns igigepseps ellrtkasvp vvapvnihgg ggsrselvit


721
wesipeelqn gegfgyiimf rpvgsttwsk ekvssvessr fvyrnesiip lspfevkvgv


781
ynnegegsls tvtivysged epqlaprgts lqsfsaseme vswnaiawnr ntgrvlgyev


841
lywtddskes migkirvsgn vttknitglk antiyfasvr ayntagtgps sppvnvttkk


901
sppsqppani awkltnsklc lnwehvktme nesevlgyki lyrqnrqskt hiletnntsa


961
ellvpfeedy lieirtvsdg gdgssseeir ipkmsslssr giqflepsth flsivivifh


1021
cfaiqpli


 


Gene ID:
X21


Gene symbol:
CNTN6


Gene description:
Contactin 6


Unigene:
Hs.387300


Genbank:
AB003592


Entrez Gene:
27255


Refseq:
NM_014461







Protein sequence (SEQ ID NO (44)):








1
marfglpall ctlavlsaal laaelksksc sevrrlyvsk gfnkndaplh eingdhlkic


61
pqgstccsqe meekyslqsk ddfksvvseq cnhlqavfas rykkfdeffk ellenaeksl


121
ndmfvktygh lymqnselfk dlfvelkryy vvgnvnleem lndfwarlle rmfrlvnsqy


181
hftdeylecv skyteqlkpf gdvprklklq vtrafvaart faqglavagd vvskvsvvnp


241
taqcthallk miycshcrgl vtvkpcynyc snimrgclan qgdldfewnn fidamlmvae


301
rlegpfnies vmdpidvkis daimnmqdns vqvsqkvfqg cgppkplpag risrsisesa


361
fsarfrphhp eerpttaagt sldrlvtdvk eklkqakkfw sslpsnvcnd ermaagngne


421
ddcwngkgks rylfavtgng lanqgnnpev qvdtskpdil ilrqimalrv mtskmknayn


481
gndvdffdis dessgegsgs gceyqqcpse fdynatdhag ksanekadsa gvrpgaqayl


541
ltvfcilflv mqrewr


 


Gene ID:
X22


Gene symbol:
SLC9A7


Gene description:
solute carrier family 9 (sodium/hydrogen exchanger), member 7


Unigene:
Hs.496057


Genbank:
AF298591


Entrez Gene:
84679


Refseq:
NM_001257291 | NM_032591







Protein sequence isoform 1 (SEQ ID NO (45)):








1
mepgdaarpg sgratgappp rllllplllg wglrvaaaas asssgaaaed ssameelate


61
keaeeshrqd syslltfill ltltiltiwl fkhrrvrflh etglamiygl ivgvilrygt


121
patsgrdksl sctqedrafs tllvnvsgkf feytlkgeis pgkinsveqn dmlrkvtfdp


181
evffnillpp iifhagyslk krhffrnlgs ilayaflgta vscfiignlm ygvvklmkim


241
gqlsdkfyyt dclffgaiis atdpvtvlai fnelhadvdl yallfgesvl ndavaivlss


301
sivayqpagl nthafdaaaf fksvgiflgi fsgsftmgav tgvvtalvtk ftklhcfpll


361
etalfflmsw stfllaeacg ftgvvavlfc gitqahytyn nlsvesrsrt kqlfevlhfl


421
aenfifsymg lalftfqkhv fspifiigaf vaiflgraah iyplsfflnl grrhkigwnf


481
qhmmmfsglr gamafalair dtasyarqmm ftttllivff tvwiigggtt pmlswlnirv


541
gveepseedq nehhwqyfrv gvdpdqdppp nndsfqvlqg dgpdsargnr tkqesawifr


601
lwysfdhnyl kpilthsgpp ltttlpawcg llarcltspq vydnqeplre edsdfilteg


661
dltltygdst vtangssssh tastslegsr rtkssseevl erdlgmgdqk vssrgtrlvf


721
pledna


 







Protein sequence isoform 2 (SEQ ID NO (46)):








1
mepgdaarpg sgratgappp rllllplllg wglrvaaaas asssgaaaed ssameelate


61
keaeeshrqd syslltfill ltltiltiwl fkhrrvrflh etglamiygl ivgvilrygt


121
patsgrdksl sctqedrafs tllvnvsgkf feytlkgeis pgkinsveqn dmlrkvtfdp


181
evffnillpp iifhagyslk krhffrnlgs ilayaflgta vscffignlm ygvvklmkim


241
gqlsdkfyyt dclffgaiis atdpvtvlai fnelhadvdl yallfgesvl ndavaivlss


301
sivayqpagl nthafdaaaf fksvgiflgi fsgsftmgav tgvnanvtkf tklhcfplle


361
talfflmsws tfllaeacgf tgvvavlfcg itqahytynn lsvesrsrtk qlfevlhfla


421
enfifsymgl alftfqkhvf spifiigafv aiflgraahi yplsfflnlg rrhkigwnfq


481
hmmmfsglrg amafalaird tasyarqmmf tttllivfft vwiigggttp mlswlnirvg


541
veepseedqn ehhwqyfrvg vdpdqdpppn ndsfqvlqgd gpdsargnrt kqesawifrl


601
wysfdhnylk pilthsgppl tttlpawcgl larcltspqv ydnqeplree dsdfiltegd


661
ltltygdstv tangssssht astslegsrr tkssseevle rdlgmgdqkv ssrgtrlvfp


721
ledna


 


Gene ID:
X23


Gene symbol:
PVRL3


Gene description:
poliovirus receptor-related 3


Unigene:
Hs.293917


Genbank:
AK075105


Entrez Gene:
25945


Refseq:
NM_015480 | NM_001243286 | NM_001243288







Protein sequence isoform 1 (SEQ ID NO (47)):








1
martlrpspl cpgggkaqls sasllgagll lqpptpppll lllfplllfs rlcgalagpi


61
ivephvtavw gknvslkcli evnetitqis wekihgkssq tvavhhpqyg fsvqgeyqgr


121
vlfknyslnd atitlhnigf sdsgkyicka vtfplgnaqs sttvtvlvep tvslikgpds


181
lidggnetva aiciaatgkp vahidwegdl gemestttsf pnetatiisq yklfptrfar


241
grritcvvkh palekdirys fildiqyape vsvtgydgnw fvgrkgvnlk cnadanpppf


301
ksvwsrldgq wpdgllasdn tlhfvhpltf nysgvyickv tnslgqrsdq kviyisdppt


361
tttlqptiqw hpstadiedl atepkklpfp lstlatikdd tiatiiasvv ggalfivlvs


421
vlagifcyrr rrtfrgdyfa knyippsdmq kesqidvlqq deldsypdsv kkenknpvnn


481
lirkdyleep ektqwnnven lnrferpmdy yedlkmgmkf vsdehydene ddlvshvdgs


541
visrrewyv


 







Protein sequence isoform 2 (SEQ ID NO (48)):








1
martlrpspl cpgggkaqls sasllgagll lqpptpppll lllfplllfs rlcgalagpi


61
ivephvtavw gknvslkcli evnetitqis wekihgkssq tvavhhpqyg fsvqgeyqgr


121
vlfknyslnd atitlhnigf sdsgkyicka vtfplgnaqs sttvtvlvep tvslikgpds


181
lidggnetva aiciaatgkp vahidwegdl gemestttsf pnetatiisq yklfptrfar


241
grritcvvkh palekdirys fildiqyape vsvtgydgnw fvgrkgvnik cnadanpppf


301
ksvwsrldgq wpdgilasdn tlhfvhpltf nysgvyickv tnslgqrsdq kviyisayns


361
vaslnc


 







Protein sequence isoform 3 (SEQ ID NO (49)):








1
maegwrwcfv rrtpgllrgp llprsfsgnp ralagpiive phvtavwgkn vslkclievn


61
etitqiswek ihgkssqtva vhhpqygfsv qgeyqgrvlf knyslndati tlhnigfsds


121
gkyickavtf plgnaqsstt vtvlveptvs likgpdslid ggnetvaaic iaatgkpvah


181
idwegdlgem estttsfpne tatiisqykl fptrfargrr itcvvkhpal ekdirysfil


241
diqyapevsv tgydgnwfvg rkgvnlkcna danpppfksv wsrldgqwpd gllasdntlh


301
fvhpltfnys gvyickvtns lgqrsdqkvi yisdvpfkqt ssiavagavi gavlalfiia


361
ifvtvlltpr kkrpsyldkv idlppthkpp plyeersppl pqkdlfqpeh lplqtqfker


421
evgnlqhsng lnsrsfdyed enpvgedgiq qmyplynqmc yqdrspgkhh qnndpkrvyi


481
dprehyv


 


Gene ID:
X24


Gene symbol:
SLC4A4


Gene description:
solute carrier family 4, sodium bicarbonate cotransporter, member 4


Unigene:
Hs.5462


Genbank:
AF011390


Entrez Gene:
8671


Refseq:
NM_001098484 | NM_001134742 | NM_003759







Protein sequence isoform 1 (SEQ ID NO (50)):








1
medeavldrg asflkhvcde eeveghhtiy igvhvpksyr rrrrhkrktg hkekkekeri


61
senysdksdi enadessssi lkplispaae rirfilgeed dspappqlft eldellavdg


121
qemewketar wikfeekveq ggerwskphv atlslhslfe lrtcmekgsi mldreasslp


181
qlvemivdhq ietgllkpel kdkvtytllr khrhqtkksn lrsladigkt vssasrmftn


241
pdngspamth rnltssslnd isdkpekdql knkfmkklpr daeasnvlvg evdfldtpfi


301
afvrlqqavm lgaltevpvp trfifillgp kgkaksyhei graiatlmsd evfhdiayka


361
kdrhdliagi defldevivl ppgewdpair ieppkslpss dkrknmysgg envqmngdtp


421
hdgghggggh gdceelqrtg rfcgglikdi krkapffasd fydalniqal sailfiylat


481
vtnaitfggl lgdatdnmqg vlesflgtav sgaifclfag qpltilsstg pvlvferlif


541
nfskdnnfdy lefriwiglw saficlilva tdasflvqyf trfteegfss lisfifiyda


601
fkkmiklady ypinsnfkvg yntlfsctcv ppdpanisis ndttlapeyl ptmsstdmyh


661
nttfdwafls kkecskyggn lvgnnenfvp ditlmsfilf lgtytssmal kkfktspyfp


721
ttarklisdf aiilsilifc vidalvgvdt pklivpsefk ptspnrgwfv ppfgenpwwv


781
claaaipall vtilifmdqq itavivnrke hklkkgagyh ldlfwvailm vicslmalpw


841
yvaatvisia hidslkmete tsapgeqpkf lgvreqrvtg tlvfiltgls vfmapilkfi


901
pmpvlygvfl ymgvaslngv qfmdrlklll mplkhqpdfi ylrhyplrrv hlftflqvlc


961
lallwilkst vaaiifpvmi lalvavrkgm dylfsqhdls flddvipekd kkkkedekkk


1021
kkkkgsldsd nddsdcpyse kvpsikipmd imeqqpflsd skpsdrersp tflerhtsc


 







Protein sequence isoform 3 (SEQ ID NO (51)):








1
medeavldrg asflkhvcde eeveghhtiy igvhvpksyr rrrrhkrktg hkekkekeri


61
senysdksdi enadessssi lkplispaae rirfilgeed dspappqlft eldellavdg


121
qemewketar wikfeekveq ggerwskphv atlslhslfe lrtcmekgsi mldreasslp


181
qlvemivdhq ietgllkpel kdkvtytllr khrhqtkksn lrsladigkt vssasrmftn


241
pdngspamth rnltssslnd isdkpekdql knkfmkklpr daeasnvlvg evdfldtpfi


301
afvrlqqavm lgaltevpvp trflfillgp kgkaksyhei graiatlmsd evfhdiayka


361
kdrhdliagi defidevivi ppgewdpair ieppkslpss dkrknmysgg envqmngdtp


421
hdgghggggh gdceelqrtg rfcgglikdi krkapffasd fydalniqal sailfiylat


481
vtnaitfggl lgdatdnmqg vlesflgtav sgaifclfag qpltilsstg pvlvferllf


541
nfskdnnfdy lefrlwiglw saflclilva tdasflvqyf trfteegfss lisfifiyda


601
fkkmiklady ypinsnfkvg yntlfsctcv ppdpanisis ndttlapeyl ptmsstdmyh


661
nttfdwafls kkecskyggn lvgnnenfvp ditlmsfilf lgtytssmal kkfktspyfp


721
ttarklisdf aiilsilifc vidalvgvdt pklivpsefk ptspnrgwfv ppfgenpwwv


781
claaaipall vtilifmdqq itavivnrke hklkkgagyh ldlfwvailm vicslmalpw


841
yvaatvisia hidslkmete tsapgeqpkf lgvreqrvtg tlvfiltgls vfmapilkfi


901
pmpvlygvfl ymgvaslngv qfmdrlklll mplkhqpdfi ylrhyplrrn hlftflqvlc


961
lallwilkst vaaiifpvmi lalvavrkgm dylfsqhdls flddvipekd kkkkedekkk


1021
kkkkgsldsd nddekdhqhs lnathhadki pflqsigmps pprtpvkvvp qirieleped


1081
ndyfwrskgt ettl


 







Protein sequence isoform 2 (SEQ ID NO (52)):








1
mstenvegkp snlgergrar sstflrvvqp mfnhsiftsa vspaaerirf ilgeeddspa


61
ppqlftelde llavdgqeme wketarwikf eekveqgger wskphvatls lhslfelrtc


121
mekgsimldr easslpqlve mivdhqietg llkpelkdkv tytllrkhrh qtkksnlrsl


181
adigktvssa srmftnpdng spamthrnlt ssslndisdk pekdqlknkf mkklprdaea


241
snvlvgevdf ldtpfiafvr lqqavmlgal tevpvptrfl fillgpkgka ksyheigrai


301
atlmsdevfh diaykakdrh dliagidefl devivlppge wdpairiepp kslpssdkrk


361
nmysggenvq mngdtphdgg hgggghgdce elqrtgrfcg glikdikrka pffasdfyda


421
lniqalsail fiylatvtna itfggllgda tdnmqgvles flgtaysgai fclfagqplt


481
ilsstgpvlv ferllfnfsk dnnfdylefr lwiglwsafl clilvatdas flvqyftrft


541
eegfsslisf ifiydafkkm ikladyypin snfkvgyntl fsctcvppdp anisisndtt


601
lapeyiptms stdmyhnttf dwaflskkec skyggnlvgn nenfvpditl msfilflgty


661
tssmalkkfk tspyfpttar klisdfaiil silifcvida lvgvdtpkli vpsefkptsp


721
nrgwfvppfg enpwwvclaa aipallvtil ifmdqqitav ivnrkehklk kgagyhldlf


781
wvailmvics lmalpwyvaa tvisiahids lkmetetsap geqpkflgvr eqrvtgtlvf


841
iltglsvfma pilkfipmpv lygvflymgv aslngvqfmd rlklllmplk hqpdfiylrh


901
vplrrvhlft flqvlclall wilkstvaai ifpvmilalv avrkgmdylf sqhdlsfldd


961
vipekdkkkk edekkkkkkk gsldsdndds dcpysekvps ikipmdimeq qpflsdskps


1021
drersptfle rhtsc


 


Gene ID:
X25


Gene symbol:
CXADR


Gene description:
coxsackie virus and adenovirus receptor


Unigene:
Hs.634837


Genbank:
AY072912 | AY072911 | AY072910 | AK313526 |


Entrez Gene:
1525


Refseq:
NM_001338 | NM_001207063 | NM_001207064 | NM_001207065 |



NM_001207066







Protein sequence isoform 1 (SEQ ID NO (53)):








1
malllcfvll cgvvdfarsl sittpeemie kakgetaylp ckftlspedq gpldiewlis


61
padnqkvdqv iilysgdkiy ddyypdlkgr vhftsndlks gdasinvtnl qlsdigtyqc


121
kvkkapgvan kkihlvvlvk psgarcyvdg seeigsdfki kcepkegslp lqyewqklsd


181
sqkmptswla emtssvisvk nasseysgty sctvrnrvgs dqcllrlnvv ppsnkaglia


241
gaiigtllal aligliifcc rkkrreekye kevhhdired vpppksrtst arsyigsnhs


301
slgsmspsnm egysktqynq vpsedfertp qsptlppakv aapnlsrmga ipvmipaqsk


361
dgsiv


 







Protein sequence isoform 2 (SEQ ID NO (54)):








1
malllcfvll cgvvdfarsl sittpeemie kakgetaylp ckftlspedq gpldiewlis


61
padnqkvdqv iilysgdkiy ddyypdlkgr vhftsndlks gdasinvtnl qlsdigtyqc


121
kvkkapgvan kkihlvvlvk psgarcyvdg seeigsdfki kcepkegslp lqyewqklsd


181
sqkmptswla gkmchlqrav rplpeatsav iihpwgpcll ptwkdiprls itkyqvktln


241
allrvrlshl lr


 







Protein sequence isoform 3 (SEQ ID NO(55)):








1
malllcfvll cgvvdfarsl sittpeemie kakgetaylp ckftlspedq gpldiewlis


61
padnqkvdqv iilysgdkiy ddyypdlkgr vhftsndlks gdasinvtnl qlsdigtyqc


121
kvkkapgvan kkihlvvlgk mchlqravrp lpeatsavii hpwgpcllpt wkdiprlsit


181
kyqvktlnal lrvrlshllr


 







Protein sequence isoform 4 (SEQ ID NO (56)):








1
malllcfvll cgnndfarsl sittpeemie kakgetaylp ckftlspedq gpldiewlis


61
padnqkvdqv grcatskepy vhcqklhrq


 







Protein sequence isoform 5 (SEQ ID NO(57)):








1
malllcfvll cgvvdfarsl sittpeemie kakgetaylp ckftlspedq gpldiewlis


61
padnqkvdqv iilysgdkiy ddyypdlkgr vhftsndlks gdasinvtnl qlsdigtyqc


121
kvkkapgvan kkihlvvlvk psgarcyvdg seeigsdfki kcepkegslp lqyewqklsd


181
sqkmptswla emtssvisvk nasseysgty sctvrnrvgs dqcllrlnvv ppsnkaglia


241
gaiigtllal aligliifcc rkkrreekye kevhhdired vpppksrtst arsyigsnhs


301
slgsmspsnm egysktqynq vpsedfertp qsptlppakf kypyktdgit vv


 


Gene ID:
X26


Gene symbol:
CADM4


Gene description:
cell adhesion molecule 4


Unigene:
Hs.370984


Genbank:
AF363368


Entrez Gene:
199731


Refseq:
NM_145296







Protein sequence (SEQ ID NO (58)):








1
mgrarrfqwp llllwaaaag pgagqevqte nvtvaeggva eitcrlhqyd gsivviqnpa


61
rqtlffngtr alkderfqle efsprrvrir lsdarledeg gyfcqlyted thhqiatltv


121
lvapenpvve vreqavegge velsclvprs rpaatlrwyr drkelkgvss sqengkvwsv


181
astvrfrvdr kddggiiice aqnqalpsgh skqtqyvldv qysptariha sqavvregdt


241
lvltcavtgn prpnqirwnr gneslperae avgetltlpg lvsadngtyt ceasnkhgha


301
ralyvlvvyd pgavveaqts vpyaivggil allvfliicv lvgmvwcsvr qkgsylthea


361
sgldeqgear eaflngsdgh krkeeffi


 


Gene ID:
Y1


Gene symbol:
CLCA2


Gene description:
chloride channel accessory 2


Unigene:
Hs.241551


Genbank:
BC041096


Entrez Gene:
9635


Refseq:
NM_006536







Protein sequence (SEQ ID NO (59)):








1
mtqrsiagpi cnlkfvtllv alsselpflg agvqlqdngy nglliainpq vpenqnlisn


61
ikemiteasf ylfnatkrrv ffrnikilip atwkannnsk ikqesyekan vivtdwygah


121
gddpytlqyr gcgkegkyih ftpnfllndn ltagygsrgr vfvhewahlr wgvfdeynnd


181
kpfyingqnq ikvtrcssdi tgifvcekgp cpqenciisk lfkegctfiy nstqnatasi


241
mfmqslssvv efcnasthnq eapnlqnqmc slrsawdvit dsadfhhsfp mngtelpppp


301
tfslvqagdk vvclvldvss kmaeadrllq lqqaaefylm qiveihtfvg iasfdskgei


361
raqlhqinsn ddrkllvsyl pttvsaktdi sicsglkkgf evveklngka ygsvmilvts


421
gddkllgncl ptvlssgsti hsialgssaa pnleelsrlt gglkffvpdi snsnsmidaf


481
srissgtgdi fqqhiqlest genvkphhql kntvtvdntv gndtmflvtw qasgppeiil


541
fdpdgrkyyt nnfitnitfr taslwipgta kpghwtytln nthhslqalk vtvtsrasns


601
avppatveaf verdslhfph pvmiyanvkq gfypilnatv tatvepetgd pvtlrllddg


661
agadvikndg iysryffsfa angryslkvh vnhspsistp ahsipgsham yvpgytangn


721
iqmnaprksv grneeerkwg fsrvssggsf svlgvpagph pdvfppckii dleavkveee


781
ltlswtapge dfdqgqatsy eirmskslqn iqddfnnail vntskrnpqq agireififs


841
pqistngpeh qpngethesh riyvairamd rnslqsaysn iaqaplfipp nsdpvpardy


901
lilkgvltam gligiiclii wthhtlsrk kradkkengt kll


 


Gene ID:
Y2


Gene symbol:
ECM1


Gene description:
extracellular matrix protein 1


Unigene:
Hs.81071


Genbank:
U68187 | U68186 | AK097046


Entrez Gene:
1893


Refseq:
NM_004425 | NM_022664 | NM_001202858







Protein sequence isoform 1 (SEQ ID NO (60)):








1
mgttaraalv ltylavasaa seggftatgq rqlrpehfqe vgyaappspp lsrslpmdhp


61
dssqhgppfe gqsqvqppps qeatplqqek llpaqlpaek evgpplpqea vplqkelpsl


121
qhpneqkegt papfgdqshp epeswnaaqh cqqdrsqggw ghrldgfppg rpspdnlnqi


181
clpnrqhvvy gpwnlpqssy shltrqgetl nfleigysrc chcrshtnrl ecaklvweea


241
msrfceaefs vktrphwcct rqgearfscf qeeapqphyq lracpshqpd issglelpfp


301
pgyptldnik nichlrrfrs vprnlpatdp lqrellaliq lerefqrccr qgnnhtctwk


361
awedtldkyc dreyavkthh hlccrhppsp trdecfarra pypnydrdil tidigrvtpn


421
lmghlegnqr vltkhkhipg lihnmtarcc dlpfpeqacc aeeekltfin dlcgprrniw


481
rdpalccyls pgdeqvncfn inylrnvalv sgdtenakgq geqgstggtn isstsepkee


 







Protein sequence isoform 2 (SEQ ID NO (61)):








1
mgttaraalv ltylavasaa seggftatgq rqlrpehfqe vgyaappspp lsrslpmdhp


61
dssqhgppfe gqsqvqppps qeatplqqek llpaqlpaek evgpplpqea vplqkelpsl


121
qhpneqkegt papfgdqshp epeswnaaqh cqqdrsqggw ghrldgfppg rpspdnlnqi


181
clpnrqhvvy gpwnlpqssy shltrqgetl nfleigysrc chcrshtnrl ecaklvwedt


241
ldkycdreya vkthhhlccr hppsptrdec farrapypny drdiltidig rvtpnlmghl


301
cgnqrvltkh khipglihnm tarccdlpfp eqaccaeeek ltfindlcgp rrniwrdpal


361
ccylspgdeq vncfninylr nvalvsgdte nakgqgeqgs tggtnissts epkee


 







Protein sequence isoform 3 (SEQ ID NO (62)):








1
mgttaraalv ltylavasaa seggftatgq rqlrpehfqe vgyaappspp lsrslpmdhp


61
dssqhgppfe gqsgkegrgp rphsqpwlge rvgcshipps ivqpppsqea tplqqekllp


121
aqlpaekevg pplpqeavpl qkelpslqhp neqkegtpap fgdqshpepe swnaaqhcqq


181
drsqggwghr ldgfppgrps pdnlnqiclp nrqhvvygpw nlpqssyshl trqgetlnfl


241
eigysrcchc rshtnrleca klvweeamsr fceaefsvkt rphwcctrqg earfscfqee


301
apqphyqlra cpshqpdiss glelpfppgv ptldniknic hlrrfrsvpr nlpatdplqr


361
ellaliqler efqrccrqgn nhtctwkawe dtldkycdre yavkthhhlc crhppsptrd


421
ecfarrapyp nydrdiltid igrvtpnlmg hlcgnqrvlt khkhipglih nmtarccdlp


481
fpeqaccaee ekltfindlc gprrniwrdp alccylspgd eqvncfniny lrnvalvsgd


541
tenakgqgeq gstggtniss tsepkee


 


Gene ID:
Y3


Gene symbol:
CLDN1


Gene description:
claudin 1


Unigene:
Hs.439060


Genbank:
AY358652


Entrez Gene:
9076


Refseq:
NM_021101







Protein sequence (SEQ ID NO (63)):








1
managlqllg filaflgwig aivstalpqw riysyagdni vtaqamyegl wmscvsqstg


61
qiqckvfdsl lnlsstlqat ralmvvgill gviaifvatv gmkcmkcled devqkmrmav


121
iggaifflag lailvatawy gnrivqefyd pmtpvnarye fgqalftgwa aaslcllgga


181
llccscprkt tsyptprpyp kpapssgkdy v


 


Gene ID:
Y4


Gene symbol:
SFN


Gene description:
stratifin


Unigene:
Hs.523718


Genbank:
AF029082


Entrez Gene:
2810


Refseq:
NM_006142







Protein sequence (SEQ ID NO (64)):








1
merasliqka klaeqaerye dmaafmkgav ekgeelscee rnllsvaykn vvggqraawr


61
vlssieqksn eegseekgpe vreyrekvet elqgvcdtvl glldshlike agdaesrvfy


121
lkmkgdyyry laevatgddk kriidsarsa yqeamdiskk empptnpirl glalnfsvfh


181
yeianspeea islakttfde amadlhtlse dsykdstlim ql1rdnltlw tadnageegg


241
eapqepqs


 


Gene ID:
Y5


Gene symbol:
CD9


Gene description:
CD9 antigen


Unigene:
Hs.114286


Genbank:
AY966455


Entrez Gene:
928


Refseq:
NM_001769







Protein sequence (SEQ ID NO (65)):








1
mpvkggtkci kyllfgfnfi fwlagiavla iglwlrfdsq tksifeqetn nnnssfytgv


61
yiligagalm mlvgflgccg avqesqcmlg lffgfllvif aieiaaaiwg yshkdevike


121
vqefykdtyn klktkdepqr etlkaihyal nccglaggve qfisdicpkk dvletftvks


181
cpdaikevfd nkfhiigavg igiawmifg mifsmilcca irmremv


 


Gene ID:
Y6


Gene symbol:
CD109


Gene description:
CD109 antigen


Unigene:
Hs.399891


Genbank:
AF410459


Entrez Gene:
135228


Refseq:
NM_133493 | NM_001159587 | NM_001159588







Protein sequence isoform 1 (SEQ ID NO (66)):








1
mqgpplltaa hllcvctaal avapgprflv tapgiirpgg nvtigvelle hcpsqvtvka


61
ellktasnit vsvleaegvf ekgsfktltl pslplnsade iyelrvtgrt qdeilfsnst


121
rlsfetkris vfiqtdkaly kpkqevkfri vtlfsdfkpy ktslnilikd pksnliqqwl


181
sqqsdlgvis ktfqlsshpi lgdwsiqvqv ndqtyyqsfq vseyvlpkfe vtlqtplycs


241
mnskhlngti takytygkpv kgdvtltflp lsfwgkkkni tktfkingsa nfsfndeemk


301
nvmdssngls eyldlsspgp veilttvtes vtgisrnvst nvffkqhdyi ieffdyttvl


361
kpslnftatv kvtradgnql tleerrnnvv itvtqrnyte ywsgsnsgnq kmeavqkiny


421
tvpqsgtfki efpiledsse lqlkayflgs kssmavhslf kspsktyiql ktrdenikvg


481
spfelvvsgn krlkelsymv vsrgqlvavg kqnstmfslt penswtpkac vivyyieddg


541
eiisdvlkip vqlvfknkik lywskvkaep sekvslrisv tqpdsivgiv avdksvnlmn


601
asnditmenv vhelelyntg yylgmfmnsf avfqecglwv ltdanltkdy idgvydnaey


661
aerfmeeneg hivdihdfsl gssphvrkhf petwiwldtn mgyriyqefe vtvpdsitsw


721
vatgfvised lglgltttpv elqafqpffi flnlpysvir geefaleiti fnylkdatev


781
kviieksdkf dilmtsnein atghqqtllv psedgatvlf pirpthlgei pitvtalspt


841
asdavtqmil vkaegieksy sqsilldltd nrlqstlktl sfsfppntvt gservqitai


901
gdvlgpsing laslirmpyg cgeqnminfa pniyildylt kkkqltdnlk ekalsfmrqg


961
yqrellyqre dgsfsafgny dpsgstwlsa fvlrcflead pyididqnvl hrtytwlkgh


1021
qksngefwdp grvihselqg gnkspvtlta yivtsllgyr kyqpnidvqe sihflesefs


1081
rgisdnytla lityalssvg spkakealnm ltwraeqegg mqfwvssesk lsdswqprsl


1141
dievaayall shflqfqtse gipimrwlsr qrnslggfas tqdttvalka lsefaalmnt


1201
ertniqvtvt gpsspspvkf lidthnrlll qtaelavvqp tavnisangf gfaicqlnvv


1261
ynvkasgssr rrrsiqnqea fdldvavken kddlnhvdln vctsfsgpgr sgmalmevnl


1321
lsgfmvpsea islsetvkkv eydhgklnly ldsvnetqfc vnipavrnfk vsntqdasys


1381
ivdyyeprrq avrsynsevk lsscdlcsdv qgcrpcedga sgshhhssvi fifcfkllyf


1441
melwl


 







Protein sequence isoform 2 (SEQ ID NO (67)):








1
mqgpplltaa hllcvctaal avapgprflv tapgiirpgg nvtigvelle hcpsqvtvka


61
ellktasnit vsvleaegvf ekgsfktltl pslplnsade iyelrvtgrt qdeilfsnst


121
rlsfetkris vfiqtdkaly kpkqevkfri vtlfsdfkpy ktslnilikd pksnliqqwl


181
sqqsdlgvis ktfqlsshpi lgdwsiqvqv ndqtyyqsfq vseyvlpkfe vtlqtplycs


241
mnskhlngti takytygkpv kgdvtltflp lsfwgkkkni tktfkingsa nfsfndeemk


301
nvmdssngls eyldlsspgp veilttvtes vtgisrnvst nvffkqhdyi ieffdyttvl


361
kpslnftatv kvtradgnql tleerrnnvv itvtqrnyte ywsgsnsgnq kmeavqkiny


421
tvpqsgtfki efpiledsse lqlkayflgs kssmavhslf kspsktyiql ktrdenikvg


481
spfelvvsgn krlkelsymv vsrgqlvavg kqnstmfslt penswtpkac vivyyieddg


541
eiisdvlkip vqlvfknkik lywskvkaep sekvslrisv tqpdsivgiv avdksvnlmn


601
asnditmenv vhelelyntg yylgmfmnsf avfqecglwv ltdanltkdy idgvydnaey


661
aerfmeeneg hivdihdfsl gssphyrkhf petwiwldtn mgyriyqefe vtvpdsitsw


721
vatgfvised lglgltttpv elqafqpffi flnlpysvir geefaleiti fnylkdatev


781
kviieksdkf dilmtsnein atghqqtllv psedgatvlf pirpthlgei pitvtalspt


841
asdavtqmil vkaegieksy sqsilldltd nrlqstlktl sfsfppntvt gservqitai


901
gdvlgpsing laslirmpyg cgeqnminfa pniyildylt kkkqltdnlk ekalsfmrqg


961
yqrellyqre dgsfsafgny dpsgstwlsa fvlrcflead pyididqnvl hrtytwlkgh


1021
qksngefwdp grvihselqg gnkspvtlta yivtsllgyr kyqpnidvqe sihflesefs


1081
rgisdnytla lityalssvg spkakealnm ltwraeqegg mqfwvssesk lsdswqprsl


1141
dievaayall shflqfqtse gipimrwlsr qrnslggfas tqdttvalka lsefaalmnt


1201
ertniqvtvt gpsspsplav vqptavnisa ngfgfaicql nvvynvkasg ssrrrrsiqn


1261
qeafdldvav kenkddlnhv dlnvctsfsg pgrsgmalme vnllsgfmvp seaislsetv


1321
kkveydhgkl nlyldsvnet qfcvnipavr nfkvsntqda sysivdyyep rrqavrsyns


1381
evklsscdlc sdvqgcrpce dgasgshhhs svififcfkl lyfmelwl


 







Protein sequence isoform 3 (SEQ ID NO (68)):








1
mqgpplltaa hllcvctaal avapgprflv tapgiirpgg nvtigvelle hcpsqvtvka


61
ellktasnit vsvleaegvf ekgsfktltl psdpksnliq qwlsqqsdlg viskifqlss


121
hpilgdwsiq vqvndqtyyq sfqvseyvlp kfevtlqtpl ycsmnskhln gtitakytyg


181
kpvkgdvtlt flplsfwgkk knitktfkin gsanfsfnde emknvmdssn glseyldlss


241
pgpveilttv tesvtgisrn vstnvffkqh dyiieffdyt tvlkpslnft atvkvtradg


301
nqltleerrn nvvitvtqrn yteywsgsns gnqkmeavqk inytvpqsgt fkiefpiled


361
sselqlkayf lgskssmavh slfkspskty iqlktrdeni kvgspfelvv sgnkrlkels


421
ymvvsrgqlv avgkqnstmf sltpenswtp kacvivyyie ddgeiisdvl kipvqlvfkn


481
kiklywskvk aepsekvslr isvtqpdsiv givavdksvn lmnasnditm envvhelely


541
ntgyylgmfm nsfavfqecg lwvltdanlt kdyidgvydn aeyaerfmee neghivdihd


601
fslgssphyr khfpetwiwl dtnmgyriyq efevtvpdsi tswvatgfvi sedlglgltt


661
tpvelqafqp ffiflnlpys virgeefale itifnylkda tevkviieks dkfdilmtsn


721
einatghqqt llvpsedgat vlfpirpthl geipitvtal sptasdavtq milvkaegie


781
ksysqsilld ltdnrlqstl ktlsfsfppn tvtgservqi taigdvlgps inglaslirm


841
pygcgeqnmi nfapniyild yltkkkqltd nlkekalsfm rqgyqrelly qredgsfsaf


901
gnydpsgstw lsafvlrcfl eadpyididq nvlhrtytwl kghqksngef wdpgrvihse


961
lqggnkspvt ltayivtsll gyrkyqpnid vqesihfles efsrgisdny tlalityals


1021
svgspkakea lnmltwraeq eggmqfwvss esklsdswqp rsldievaay allshflqfq


1081
tsegipimrw lsrqrnslgg fastqdttva lkalsefaal mntertniqv tvtgpsspsp


1141
vkflidthnr lllqtaelav vqptavnisa ngfgfaicql nvvynvkasg ssrrrrsiqn


1201
qeafdldvav kenkddlnhv dlnvctsfsg pgrsgmalme vnllsgfmvp seaislsetv


1261
kkveydhgkl nlyldsvnet qfcvnipavr nfkvsntqda sysivdyyep rrqavrsyns


1321
evklsscdlc sdvqgcrpce dgasgshhhs svififcfkl lyfmelwl


 


Gene ID:
Y7


Gene symbol:
ITGB8


Gene description:
integrin, beta 8


Unigene:
Hs.592171


Genbank:
M73780


Entrez Gene:
3696


Refseq:
NM_002214







Protein sequence (SEQ ID NO (69)):








1
mcgsalafft aafvclqndr rgpasflwaa wvfslvlglg qgednrcass naascarcla


61
lgpecgwcvq edfisggsrs ercdivsnli skgcsvdsie ypsvhviipt eneintqvtp


121
gevsiqlrpg aeanfmlkvh plkkypvdly ylvdvsasmh nnieklnsvg ndlsrkmaff


181
srdfrlgfgs yvdktvspyi sihperihnq csdynldcmp phgyihvlsl tenitefeka


241
vhrqkisgni dtpeggfdam lqaavceshi gwrkeakrll lvmtdqtshl aldsklagiv


301
vpndgnchlk nnvyvksttm ehpslgqlse klidnninvi favqgkqfhw ykdllpllpg


361
tiageieska anlnnlvvea yqklisevkv qvenqvqgiy fnitaicpdg srkpgmegcr


421
nvtsndevlf nvtvtmkkcd vtggknyaii kpigfnetak ihihrncscq cednrgpkgk


481
cvdetfldsk cfqcdenkch fdedqfsses ckshkdqpvc sgrgvcvcgk cschkiklgk


541
vygkycekdd fscpyhhgnl caghgeceag rcqcfsgweg drcqcpsaaa qhcvnskgqv


601
csgrgtcvcg rcectdprsi grfcehcptc ytackenwnc mqclhphnls qaildqckts


661
calmeqqhyv dqtsecfssp sylriffiif ivtfligllk vliirqvilq wnsnkiksss


721
dyrvsaskkd klilqsvctr avtyrrekpe eikmdiskln ahetfrcnf


 


Gene ID:
Y8


Gene symbol:
EMP2


Gene description:
epithelial membrane protein 2


Unigene:
Hs.531561


Genbank:
BC009687


Entrez Gene:
2013


Refseq:
NM_001424







Protein sequence (SEQ ID NO (70)):








1
mlvllaffia fhitsaallf iatvdnawwv gdeffadvwr ictnntnctv indsfqeyst


61
lqavqatmil stilcciaff ifvlqlfrlk qgerfvltsi iqlmsclcvm iaasiytdrr


121
edihdknakf ypvtregsyg ysyilawvaf actfisgmmy lilrkrk


 


Gene ID:
Y9


Gene symbol:
FGFBP1


Gene description:
fibroblast growth factor binding protein 1


Unigene:
Hs.1690


Genbank:
BC008910


Entrez Gene:
9982


Refseq:
NM_005130







Protein sequence (SEQ ID NO (71)):








1
mkicsltlls flllaaqvll vegkkkvkng lhskvvseqk dtlgntqikq ksrpgnkgkf


61
vtkdqancrw aateqeegis lkvectqldh efscvfagnp tsclklkder vywkqvarnl


121
rsqkdicrys ktavktrvcr kdfpesslkl vsstlfgntk prkektemsp rehikgkett


181
psslavtqtm atkapecved pdmanqrkta lefcgetwss lctfflsivq dtsc


 


Gene ID:
Y10


Gene symbol:
CDH3


Gene description:
cad herin 3, type 1, P-cadherin (placental)


Unigene:
Hs.191842


Genbank:
BC041846


Entrez Gene:
1001


Refseq:
NM_001793







Protein sequence (SEQ ID NO (72)):








1
mglprgplas llllqvcwlq caasepcrav freaevtlea ggaeqepgqa lgkvfmgcpg


61
qepalfstdn ddftvrnget vqerrslker nplkifpskr ilrrhkrdwv vapisvpeng


121
kgpfpqrinq lksnkdrdtk ifysitgpga dsppegvfav eketgwllln kpldreeiak


181
yelfghavse ngasvedpmn isiivtdqnd hkpkftqdtf rgsvlegvlp gtsvmqvtat


241
deddaiytyn gvvaysihsq epkdphdlmf tihrstgtis vissgldrek vpeytltiqa


301
tdmdgdgstt tavavveild andnapmfdp qkyeahvpen avghevqrlt vtdldapnsp


361
awratylimg gddgdhftit thpesnqgil ttrkgldfea knqhtlyvev tneapfvlkl


421
ptstativvh vedvneapvf vppskvvevq egiptgepvc vytaedpdke nqkisyrilr


481
dpagwlamdp dsgqvtavgt ldredeqfvr nniyevmvla mdngsppttg tgtllltlid


541
vndhgpvpep rqiticnqsp vrqvlnitdk dlsphtspfq aqltddsdiy wtaevneegd


601
tvvlslkkfl kqdtydvhls lsdhgnkeql tviratvcdc hghvetcpgp wkggfilpvl


661
gavlallfll lvllllvrkk rkikeplllp eddtrdnvfy ygeegggeed qdyditqlhr


721
glearpevvl rndvaptiip tpmyrprpan pdeignfiie nlkaantdpt appydtllvf


781
dyegsgsdaa slssltssas dqdqdydyln ewgsrfkkla dmygggedd


 


Gene ID:
Y11


Gene symbol:
ITGB4


Gene description:
integrin, beta 4


Unigene:
Hs.632226


Genbank:
X53587


Entrez Gene:
3691


Refseq:
NM_000213 | NM_001005619 | NM_001005731







Protein sequence isoform 1 (SEQ ID NO (73)):








1
magprpspwa rlllaalisv slsgtlanrc kkapvkscte cvrvdkdcay ctdemfrdrr


61
cntqaellaa gcqresivvm essfqiteet qidttlrrsq mspqglrvrl rpgeerhfel


121
evfeplespv dlyilmdfsn smsddldnlk kmgqnlarvl sqltsdytig fgkfvdkvsv


181
pqtdmrpekl kepwpnsdpp fsfknvislt edvdefrnkl qgerisgnld apeggfdail


241
qtavctrdig wrpdsthllv fstesafhye adganvlagi msrnderchl dttgtytqyr


301
tqdypsvptl vrllakhnii pifavtnysy syyeklhtyf pvsslgvlqe dssnivelle


361
eafnrirsnl diraldsprg lrtevtskmf qktrtgsfhi rrgevgiyqv qlralehvdg


421
thvcqlpedq kgnihlkpsf sdglkmdagi icdvctcelq kevrsarcsf ngdfvcgqcv


481
csegwsgqtc ncstgslsdi qpclregedk pcsgrgecqc ghcvcygegr yegqfceydn


541
fqcprtsgfl cndrgrcsmg qcvcepgwtg pscdcplsna tcidsnggic ngrghcecgr


601
chchqqslyt dticeinysa ihpglcedlr scvqcqawgt gekkgrtcee cnfkvkmvde


661
lkraeevvvr csfrdedddc tysytmegdg apgpnstvlv hkkkdcppgs fwwlipllll


721
llpllallll lcwkycacck aclallpccn rghmvgfked hymlrenlma sdhldtpmlr


781
sgnlkgrdvv rwkvtnnmqr pgfathaasi nptelvpygl slrlarlcte nllkpdtrec


841
aqlrqeveen lnevyrqisg vhklqqtkfr qqpnagkkqd htivdtvlma prsakpallk


901
ltekqveqra fhdlkvapgy ytltadqdar gmvefqegve lvdvrvplfi rpedddekql


961
lveaidvpag tatlgrrlvn itiikeqard vvsfeqpefs vsrgdqvari pvirrvldgg


1021
ksqvsyrtqd gtaqgnrdyi pvegellfqp geawkelqvk llelqevdsl lrgrqvrrfh


1081
vqlsnpkfga hlgqphstti iirdpdeldr sftsqmlssq ppphgdlgap qnpnakaags


1141
rkihfnwlpp sgkpmgyrvk ywiqgdsese ahlldskvps veltnlypyc dyemkvcayg


1201
aqgegpyssl vscrthqevp sepgrlafnv vsstvtqlsw aepaetngei tayevcyglv


1261
nddnrpigpm kkvlvdnpkn rmllienlre sqpyrytvka rngagwgper eaiinlatqp


1321
krpmsipiip dipivdaqsg edydsflmys ddvlrspsgs qrpsysddtg cgwkfepllg


1381
eeldlrrytw rlppeliprl sassgrssda eaphgppddg gaggkggslp rsatpgppge


1441
hlvngrmdfa fpgstnslhr mtttsaaayg thlsphvphr vlstsstltr dynsltrseh


1501
shsttlprdy stltsysshd srltagvpdt ptrlvfsalg ptslrvswqe prcerplqgy


1561
sveyqllngg elhrlnipnp aqtsvvvedl lpnhsyvfry raqsqegwgr eregvities


1621
qvhpqsplcp lpgsaftlst psapgplvft alspdslqls werprrpngd ivgylvtcem


1681
aqgggpataf rvdgdspesr ltvpglsenv pykfkvqart tegfgpereg iitiesqdgg


1741
pfpqlgsrag lfqhplqsey ssittthtsa tepflvdglt lgaqhleagg sltrhvtqef


1801
vsrtlttsgt lsthmdqqff qt


 







Protein sequence isoform 2 (SEQ ID NO (74)):








1
magprpspwa rlllaalisv slsgtlanrc kkapvkscte cvrvdkdcay ctdemfrdrr


61
cntqaellaa gcqresivvm essfqiteet qidttlrrsq mspqglrvil rpgeerhfel


121
evfeplespv dlyilmdfsn smsddldnlk kmgqnlarvl sqltsdytig fgkfvdkvsv


181
pqtdmrpekl kepwpnsdpp fsfknvislt edvdefrnkl qgerisgnld apeggfdail


241
qtavctrdig wrpdsthllv fstesafhye adganvlagi msrnderchl dttgtytqyr


301
tqdypsvptl vrllakhnii pifavtnysy syyeklhtyf pvsslgvlqe dssnivelle


361
eafnrirsnl diraldsprg lrtevtskmf qktrtgsfhi rrgevgiyqv qlralehvdg


421
thvcqlpedq kgnihlkpsf sdglkmdagi icdvctcelq kevrsarcsf ngdfvcgqcv


481
csegwsgqtc ncstgslsdi qpclregedk pcsgrgecqc ghcvcygegr yegqfceydn


541
fqcprtsgfl cndrgrcsmg qcvcepgwtg pscdcplsna tcidsnggic ngrghcecgr


601
chchqqslyt dticeinysa ihpglcedlr scvqcqawgt gekkgrtcee cnfkvkmvde


661
lkraeevvvr csfrdedddc tysytmegdg apgpnstvlv hkkkdcppgs fwwlipllll


721
llpllallll lcwkycacck aclallpccn rghmvgfked hymlrenlma sdhldtpmlr


781
sgnlkgrdvv rwkvtnnmqr pgfathaasi nptelvpygl slrlarlcte nllkpdtrec


841
aqlrqeveen lnevyrqisg vhklqqtkfr qqpnagkkqd htivdtvlma prsakpallk


901
ltekqveqra fhdlkvapgy ytltadqdar gmvefqegve lvdvrvplfi rpedddekql


961
lveaidvpag tatlgrrlvn itiikeqard vvsfeqpefs vsrgdqvari pvirrvldgg


1021
ksqvsyrtqd gtaqgnrdyi pvegellfqp geawkelqvk llelqevdsl lrgrqvrrfh


1081
vqlsnpkfga hlgqphstti iirdpdeldr sftsqmlssq ppphgdlgap qnpnakaags


1141
rkihfnwlpp sgkpmgyrvk ywiqgdsese ahlldskvps veltnlypyc dyemkvcayg


1201
aqgegpyssl vscrthqevp sepgrlafnv vsstvtqlsw aepaetngei tayevcyglv


1261
nddnrpigpm kkvlvdnpkn rmllienlre sqpyrytvka rngagwgper eaiinlatqp


1321
krpmsipiip dipivdaqsg edydsflmys ddvlrspsgs qrpsysddte hlvngrmdfa


1381
fpgstnslhr mtttsaaayg thlsphvphr vlstsstltr dynsltrseh shsttlprdy


1441
stltsysshg lppiwehgrs rlplswalgs rsraqmkgfp psrgprdsii lagrpaapsw


1501
gpdsrltagv pdtptrlvfs algptslrvs wqeprcerpl qgysveyqll nggelhrini


1561
pnpaqtsvvv edllpnhsyv frvraqsqeg wgreregvit iesqvhpqsp lcplpgsaft


1621
lstpsapgpl vftalspdsl qlswerprrp ngdivgylvt cemaqgggpa tafrvdgdsp


1681
esrltvpgls envpykfkvq arttegfgpe regiitiesq dggpfpqlgs raglfqhplq


1741
seyssittth tsatepflvd gltlgaqhle aggsltrhvt qefvsrtltt sgtlsthmdq


1801
qffqt


 







Protein sequence isoform 3 (SEQ ID NO (75)):








1
magprpspwa rlllaalisv slsgtlanrc kkapvkscte cvrvdkdcay ctdemfrdrr


61
cntqaellaa gcqresivvm essfqiteet qidttlrrsq mspqglrvrl rpgeerhfel


121
evfeplespv dlyilmdfsn smsddldnlk kmgqnlarvl sqltsdytig fgkfvdkvsv


181
pqtdmrpekl kepwpnsdpp fsfknvislt edvdefrnkl qgerisgnld apeggfdail


241
qtavctrdig wrpdsthllv fstesafhye adganvlagi msrnderchl dttgtytqyr


301
tqdypsvptl vrllakhnii pifavtnysy syyeklhtyf pvsslgvlqe dssnivelle


361
eafnrirsnl diraldsprg lrtevtskmf qktrtgsfhi rrgevgiyqv qlralehvdg


421
thvcqlpedq kgnihlkpsf sdglkmdagi icdvctcelq kevrsarcsf ngdfvcgqcv


481
csegwsgqtc ncstgslsdi qpclregedk pcsgrgecqc ghcvcygegr yegqfceydn


541
fqcprtsgfl cndrgrcsmg qcvcepgwtg pscdcplsna tcidsnggic ngrghcecgr


601
chchqqslyt dticeinysa ihpglcedlr scvqcqawgt gekkgrtcee cnfkvkmvde


661
lkraeevvvr csfrdedddc tysytmegdg apgpnstvlv hkkkdcppgs fwwlipllll


721
llpllallll lcwkycacck aclallpecn rghmvgfked hymlrenlma sdhldtpmlr


781
sgnlkgrdvv rwkvtnnmqr pgfathaasi nptelvpygl slrlarlcte nllkpdtrec


841
aqlrqeveen lnevyrqisg vhklqqtkfr qqpnagkkqd htivdtvlma prsakpallk


901
ltekqveqra fhdlkvapgy ytltadqdar gmvefqegve lvdvrvplfi rpedddekql


961
lveaidvpag tatlgrrlvn itiikeqard vvsfeqpefs vsrgdqvari pvirrvldgg


1021
ksqvsyrtqd gtaqgnrdyi pvegellfqp geawkelqvk llelqevdsl lrgrqvrrfh


1081
vqlsnpkfga hlgqphstti iirdpdeldr sftsqmlssq ppphgdlgap qnpnakaags


1141
rkihfnwlpp sgkpmgyrvk ywiqgdsese ahlldskvps veltnlypyc dyemkvcayg


1201
aqgegpyssl vscrthqevp sepgrlafnv vsstvtqlsw aepaetngei tayevcyglv


1261
nddnrpigpm kkvlvdnpkn rmllienlre sqpyrytvka rngagwgper eaiinlatqp


1321
krpmsipiip dipivdaqsg edydsflmys ddvlrspsgs qrpsysddte hlvngrmdfa


1381
fpgstnslhr mtttsaaayg thlsphvphr vlstsstltr dynsltrseh shsttlprdy


1441
stltsvsshd srltagvpdt ptrlvfsalg ptslrvswqe prcerplqgy sveyqllngg


1501
elhrlnipnp aqtsvvvedl lpnhsyvfrv raqsqegwgr eregvities qvhpqsplcp


1561
lpgsaftlst psapgplvft alspdslqls werprrpngd ivgylvtcem aqgggpataf


1621
rvdgdspesr ltvpglsenv pykfkvqart tegfgpereg iitiesqdgg pfpqlgsrag


1681
lfqhplqsey ssittthtsa tepflvdglt lgaqhleagg sltrhvtqef vsrtlttsgt


1741
lsthmdqqff qt


 


Gene ID:
Y12


Gene symbol:
LAMB3


Gene description:
laminin, beta 3


Unigene:
Hs.497636


Genbank:
BC075838


Entrez Gene:
3914


Refseq:
NM_000228







Protein sequence (SEQ ID NO (76)):








1
mrpffllcfa lpgllhaqqa csrgacyppv gdllvgrtrf lrasstcglt kpetyctqyg


61
ewqmkcckcd srqphnyysh rvenvasssg pmrwwqsqnd vnpvslqldl drrfqlqevm


121
mefqgpmpag mlierssdfg ktwrvyqyla adctstfprv rqgrpqswqd vrcqslpqrp


181
narlnggkvq lnlmdlvsgi patqsqkiqe vgeitnlrvn ftrlapvpqr gyhppsayya


241
vsqlrlqgsc fchghadrca pkpgasagps tavqvhdvcv cqhntagpnc ercapfynnr


301
pwrpaegqda hecqrcdcng hsetchfdpa vfaasqgayg gvcdncrdht egkncercql


361
hyfrnrrpga siqetcisce cdpdgavpga pcdpvtgqcv ckehvqgerc dlckpgftgl


421
tyanpqgchr cdcnilgsrr dmpcdeesgr clclpnvvgp kcdqcapyhw klasgqgcep


481
cacdphnsls pqcnqftgqc pcregfgglm csaaairqcp drtygdvatg cracdcdfrg


541
tegpgcdkas grclcrpglt gprcdqcqrg ycnrypvcva chpcfqtyda dlreqalrfg


601
rlrnataslw sgpgledrgl asrildaksk ieqiravlss pavteqevaq vasailslrr


661
tlqglqldlp leeetlslpr dlesldrsfn glltmyqrkr eqfekissad psgafrmlst


721
ayeqsaqaaq qvsdssrlld qlrdsrreae rlvrqagggg gtgspklval rlemsslpdl


781
tptfnklcgn srqmactpis cpgelcpqdn gtacgsrcrg vlpraggafl magqvaeqlr


841
gfnaqlqrtr qmiraaeesa sqiqssaqrl etqvsasrsq meedvrrtrl liqqvrdflt


901
dpdtdaatiq evseavlalw lptdsatvlq kmneiqaiaa rlpnvdlvls qtkqdiarar


961
rlqaeaeear srahavegqv edvvgnlrqg tvalqeaqdt mqgtsrslrl iqdrvaevqq


1021
vlrpaeklvt smtkqlgdfw trmeelrhqa rqqgaeavqa qqlaegaseq alsaqegfer


1081
ikqkyaelkd rlgqssmlge qgariqsvkt eaeelfgetm emmdrmkdme lellrgsqai


1141
mlrsadltgl ekrveqirdh ingrvlyyat ck


 


Gene ID:
Y13


Gene symbol:
CD55


Gene description:
CD55 antigen


Unigene:
Hs.126517


Genbank:
M31516


Entrez Gene:
1604


Refseq:
NM_000574







Protein sequence (SEQ ID NO (77)):








1
mtvarpsvpa alpllgelpr llllvllclp avwgdcglpp dvpnaqpale grtsfpedtv


61
itykceesfv kipgekdsvi clkgsqwsdi eefcnrscev ptrlnsaslk qpyitqnyfp


121
vgtvveyecr pgyrrepsls pkltclqnlk wstavefckk kscpnpgeir ngqidvpggi


181
lfgatisfsc ntgyklfgst ssfclisgss vqwsdplpec reiycpappq idngiiqger


241
dhygyrqsvt yacnkgftmi gehsiyctvn ndegewsgpp pecrgkslts kvpptvqkpt


301
tvnvpttevs ptsqktttkt ttpnaqatrs tpvsrttkhf hettpnkgsg ttsgttrlls


361
ghtcftltgl lgtlvtmgll t


 


Gene ID:
Y14


Gene symbol:
CLDN16


Gene description:
claudin 16


Unigene:
Hs.251391


Genbank:
BC069682


Entrez Gene:
10686


Refseq:
NM_006580







Protein sequence (SEQ ID NO (78)):








1
mtsrtpllvt aclyysycns rhlqqgvrks krpvfshcqv petqktdtrh lsgaragvcp


61
cchpdgllat mrdllqyiac ffaffsagfl ivatwtdcwm vnaddslevs tkerglwwec


121
vtnafdgirt cdeydsilae hplklvvtra lmitadilag fgfltlllgl dcvkflpdep


181
yikvricfva gatlliagtp giigsvwyav dvyverstiv lhniflgiqy kfgwscwlgm


241
agslgcflag avltcclylf kdvgpernyp yslrkaysaa gvsmaksysa prtetakmya


301
vdtrv


 


Gene ID:
Y15


Gene symbol:
LAMA3


Gene description:
laminin, alpha 3


Unigene:
Hs.436367


Genbank:
AY327115


Entrez Gene:
3909


Refseq:
NM_198129 | NM_001127717 | NM_000227 | NM_001127718







Protein sequence isoform 1 (SEQ ID NO (79)):








1
maaaarprgr algpvlpptp llllvlrvlp acgatardpg aaaglslhpt yfnlaeaari


61
watatcgerg pgegrpqpel ycklvggpta pgsghtiqgq fcdycnsedp rkahpvtnai


121
dgserwwqsp plssgtqynr vnltldlgql fhvayilikf ansprpdlwv lersvdfgst


181
yspwqyfahs kvdclkefgr eanmavtrdd dvlcvteysr ivplengevv vslingrpga


241
knftfshtlr eftkatnirl rflrtntllg hliskaqrdp tvtrryyysi kdisiggqcv


301
cnghaevcni nnpeklfrce cqhhtcgetc drcctgynqr rwrpaaweqs heceacnchg


361
hasncyydpd verqqaslnt qgiyagggvc incqhntagv nceqcakgyt rpygvpvdap


421
dgcipcscdp ehadgceqgs grchckpnfh gdncekcaig yynfpfclri pifpvstpss


481
edpvagdikg cdcnlegvlp eicdahgrcl crpgvegprc dtcrsgfysf picqacwcsa


541
lgsyqmpcss vtgqcecrpg vtgqrcdrcl sgaydfphcq gsssacdpag tinsnlgycq


601
cklhvegptc srckllywnl dkenpsgcse ckchkagtvs gtgecrqgdg dchckshvgg


661
dscdtcedgy faleksnyfg cqgcqcdigg alssmcsgps gvcqcrehvv gkvcqrpenn


721
yyfpdlhhmk yeiedgstpn grdlrfgfdp lafpefswrg yaqmtsvqnd vritlnvgks


781
sgslfrvilr yvnpgteavs ghitiypswg aaqskeiifl pskepafvtv pgngfadpfs


841
itpgiwvaci kaegvlldyl vllprdyyea svlqlpvtep cayagppqen cllyqhlpvt


901
rfpctlacea rhflldgepr pvavrqptpa hpvmvdlsgr evelhlrlri pqvghyvvvv


961
eysteaaqlf vvdvnvkssg svlagqvniy scnysvlcrs avidhmsria myelladadi


1021
qlkghmarfl lhqvciipie efsaeyvrpq vhciasygrf vnqsatcvsl ahetpptali


1081
ldvlsgrpfp hlpqqsspsv dvlpgvtlka pqnqvtlrgr vphlgryvfv ihfyqaahpt


1141
fpaqvsvdgg wpragsfhas fcphvlgcrd qviaegqief disepevaat vkvpegkslv


1201
lvrvlvvpae nydyqilhkk smdkslefit ncgknsfyld pqtasrfckn sarslvafyh


1261
kgalpcechp tgatgphcsp eggqcpcqpn vigrqctrca tghygfprck pcscgrrlce


1321
emtgqcrcpp rtvrpqcevc ethsfsfhpm agcegcncsr rgtieaampe cdrdsgqcrc


1381
kpritgrqcd rcasgfyrfp ecvpcncnrd gtepgvcdpg tgaclckenv egtecnvcre


1441
gsfhldpanl kgctscfcfg vnnqchsshk rrtkfvdmlg whletadrvd ipvsfnpgsn


1501
smvadlqelp atihsaswva ptsylgdkvs syggyltyqa ksfglpgdmv llekkpdvql


1561
tgqhmsiiye etntprpdrl hhgrvhvveg nfrhassrap vsreelmtvl srladvriqg


1621
lyftetqrlt lsevgleeas dtgsgriala veicacppay agdscqgcsp gyyrdhkgly


1681
tgrcvpcncn ghsnqcqdgs gicvncqhnt agehcercqe gyygnavhgs cracpcphtn


1741
sfatgcvvng gdvrcsckag ytgtqcerca pgyfgnpqkf ggscqpcscn sngqlgschp


1801
ltgdcinqep kdsspaeecd dcdscvmtll ndlatmgeql rlyksqlqgl sasaglleqm


1861
rhmetqakdl rnqllnyrsa isnhgskieg lereltdlnq efetlqekaq vnsrkaqtln


1921
nnvnratqsa keldvkiknv irnvhillkq isgtdgegnn vpsgdfsrew aeaqrmmrel


1981
rnrnfgkhlr eaeadkresq lllnrirtwq kthqgenngl ansirdslne yeaklsdlra


2041
rlqeaaaqak qanglngene ralgaiqrqv keinslqsdf tkylttadss llqtnialql


2101
meksqkeyek laaslnearq elsdkvrels rsagktslve eaekharslq elakqleeik


2161
rnasgdelvr cavdaataye nilnaikaae daanraasas esalqtvike dlprkaktls


2221
snsdkllnea kmtqkklkqe vspalnnlqq tlnivtvqke vidtnlttlr dglhgiqrgd


2281
idamissaks mvrkanditd evldglnpiq tdverikdty grtqnedfkk altdadnsvn


2341
kltnklpdlw rkiesinqql lplgnisdnm drireliqqa rdaaskvavp mrfngksgve


2401
vrlpndledl kgytslslfl qrpnsrengg tenmfvmylg nkdasrdyig mavvdgqltc


2461
vynlgdreae lqvdqiltks etkeavmdrv kfqriyqfar lnytkgatss kpetpgvydm


2521
dgrnsntlln ldpenvvfyv ggyppdfklp srlsfppykg cielddlnen vlslynfkkt


2581
fnlnttevep crrrkeesdk nyfegtgyar vptqphapip tfgqtiqttv drgllffaen


2641
gdrfislnie dgklmvrykl nselpkergv gdainngrdh siqikigklq krmwinvdvq


2701
ntiidgevfd fstyylggip iairerfnis tpafrgcmkn lkktsgvvrl ndtvgvtkkc


2761
sedwklvrsa sfsrggqlsf tdlglpptdh lqasfgfqtf qpsgilldhq twtrnlqvtl


2821
edgyielsts dsgspifksp qtymdgllhy vsvisdnsgl rlliddqllr nskrlkhiss


2881
srqslrlggs nfegcisnvf vqrlslspev ldltsnslkr dvslggcsln kppflmllkg


2941
strfnktktf rinqllqdtp vasprsvkvw qdacsplpkt qanhgalqfg diptshllfk


3001
lpqellkprs qfavdmqtts srglvfhtgt knsfmalyls kgrlvfalgt dgkklriksk


3061
ekcndgkwht vvfghdgekg rlvvdglrar egslpgnsti sirapvylgs ppsgkpkslp


3121
tnsfvgclkn fqldskplyt psssfgvssc lggplekgiy fseegghvvl ahsvllgpef


3181
klvfsirprs ltgilihigs qpgkhlcvyl eagkvtasmd sgaggtstsv tpkqslcdgq


3241
whsvavtikq hilhleldtd ssytagqipf ppastqeplh lggapanltt lripvwksff


3301
gclrnihvnh ipvpvteale vqgpvslngc pdq


 







Protein sequence isoform 3 (SEQ ID NO (80)):








1
maaaarprgr algpvlpptp llllvlrvlp acgatardpg aaaglslhpt yfnlaeaari


61
watatcgerg pgegrpqpel ycklvggpta pgsghtiqgq fcdycnsedp rkahpvtnai


121
dgserwwqsp plssgtqynr vnltldlgql fhvayilikf ansprpdlwv lersvdfgst


181
yspwqyfahs kvdclkefgr eanmavtrdd dvlcvteysr ivplengevv vslingrpga


241
knftfshtlr eftkatnirl rflrtntllg hliskaqrdp tvtrryyysi kdisiggqcv


301
cnghaevcni nnpeklfrce cqhhtcgetc drcctgynqr rwrpaaweqs heceacnchg


361
hasncyydpd verqqaslnt qglyagggvc incqhntagv nceqcakgyy rpygvpvdap


421
dgcipcscdp ehadgceqgs grchckpnfh gdncekcaig yynfpfclri pifpvstpss


481
edpvagdikg cdcnlegvlp eicdahgrcl crpgvegprc dtcrsgfysf picqacwcsa


541
lgsyqmpcss vtgqcecrpg vtgqrcdrcl sgaydfphcq gsssacdpag tinsnlgycq


601
cklhvegptc srckllywnl dkenpsgcse ckchkagtvs gtgecrqgdg dchckshvgg


661
dscdtcedgy faleksnyfg cqgcqcdigg alssmcsgps gvcqcrehvv gkvcqrpenn


721
yyfpdlhhmk yeiedgstpn grdlrfgfdp lafpefswrg yaqmtsvqnd vritlnvgks


781
sgslfrvilr yvnpgteavs ghitiypswg aaqskeiifl pskepafvtv pgngfadpfs


841
itpgiwvaci kaegvlldyl vllprdyyea svlqlpvtep cayagppqen cllyqhlpvt


901
rfpctlacea rhffldgepr pvavrqptpa hpvmvdlsgr evelhlrlri pqvghyvvvv


961
eysteaaqlf vvdvnvkssg svlagqvniy scnysvlcrs avidhmsria myelladadi


1021
qlkghmarfl lhqvciipie efsaeyvrpq vhciasygrf vnqsatcvsl ahetpptali


1081
ldvlsgrpfp hlpqqsspsv dvlpgvtlka pqnqvtlrgr vphlgryvfv ihfyqaahpt


1141
fpaqvsvdgg wpragsfhas fcphvlgcrd qviaegqief disepevaat vkvpegkslv


1201
lvrvlvvpae nydyqilhkk smdkslefit ncgknsfyld pqtasrfckn sarslvafyh


1261
kgalpcechp tgatgphcsp eggqcpcqpn vigrqctrca tghygfprck pcscgrrlce


1321
emtgqcrcpp rtvrpqcevc ethsfsfhpm agcegcncsr rgtieaampe cdrdsgqcrc


1381
kpritgrqcd rcasgfyrfp ecvpcncnrd gtepgvcdpg tgaclckenv egtecnvcre


1441
gsfhldpanl kgctscfcfg vnnqchsshk rrtkfvdmlg whletadrvd ipvsfnpgsn


1501
smvadlqelp atihsaswva ptsylgdkvs syggyltyqa ksfglpgdmv llekkpdvql


1561
tgqhmsiiye etntprpdrl hhgrvhvveg nfrhassrap vsreelmtvl srladvriqg


1621
lyftetqrlt lsevgleeas dtgsgriala veicacppay agdscqgcsp gyyrdhkgly


1681
tgrcvpcncn ghsnqcqdgs gicvncqhnt agehcercqe gyygnavhgs cracpcphtn


1741
sfatgcvvng gdvrcsckag ytgtqcerca pgyfgnpqkf ggscqpcscn sngqlgschp


1801
ltgdcinqep kdsspaeecd dcdscvmtll ndlatmgeql rlvksqlqgl sasaglleqm


1861
rhmetqakdl rnqllnyrsa isnhgskieg lereltdlnq efetlqekaq vnsrkaqtln


1921
nnvnratqsa keldvkiknv irnvhmlnri rtwqkthqge nnglansird slneyeakls


1981
dlrarlqeaa aqakqangln qeneralgai qrqvkeinsl qsdftkyltt adssllqtni


2041
alqlmeksqk eyeklaasln earqelsdkv relsrsagkt slveeaekha rslqelakql


2101
eeikrnasgd elvrcavdaa tayenilnai kaaedaanra asasesalqt vikedlprka


2161
ktlssnsdkl lneakmtqkk lkqevspaln nlqqtlnivt vqkevidtnl ttlrdglhgi


2221
qrgdidamis saksmvrkan ditdevldgl npiqtdveri kdtygrtqne dfkkaltdad


2281
nsvnkltnkl pdlwrkiesi nqqllplgni sdnmdrirel iqqardaask vavpmrfngk


2341
sgvevrlpnd ledlkgytsl slflqrpnsr enggtenmfv mylgnkdasr dyigmavvdg


2401
qltcvynlgd reaelqvdqi ltksetkeav mdrvkfqriy qfarlnytkg atsskpetpg


2461
vydmdgrnsn tllnldpenv vfyvggyppd fklpsrlsfp pykgcieldd lnenvlslyn


2521
fkktfnlntt evepcrrrke esdknyfegt gyarvptqph apiptfgqti qttvdrgllf


2581
faengdrfis lniedgklmv ryklnselpk ergvgdainn grdhsiqiki gklqkrmwin


2641
vdvqntiidg evfdfstyyl ggipiairer fnistpafrg cmknlkktsg vvrlndtvgv


2701
tkkcsedwkl vrsasfsrgg qlsftdlglp ptdhlqasfg fqtfqpsgil ldhqtwtrnl


2761
qvtledgyie lstsdsgspi fkspqtymdg llhyvsvisd nsglrllidd qllrnskrlk


2821
hisssrqslr lggsnfegci snvfvqrlsl spevldltsn slkrdvslgg cslnkppflm


2881
llkgstrfnk tktfrinqll qdtpvasprs vkvwqdacsp lpktqanhga lqfgdiptsh


2941
llfklpqell kprsqfavdm qttssrglvf htgtknsfma lylskgrlvf algtdgkklr


3001
ikskekcndg kwhtvvfghd gekgrlvvdg lraregslpg nstisirapv ylgsppsgkp


3061
kslptnsfvg clknfqldsk plytpsssfg vssclggple kgiyfseegg hvvlahsvll


3121
gpefklvfsi rprsltgili higsqpgkhl cvyleagkvt asmdsgaggt stsvtpkqsl


3181
cdgqwhsvav tikqhilhle ldtdssytag qipfppastq eplhlggapa nlttlripvw


3241
ksffgclrni hvnhipvpvt ealevqgpvs lngcpdq


 







Protein sequence isoform 2 (SEQ ID NO (81)):








1
mppavrrsac smgwlwifga algqclgyss qqqrvpflqp pgqsqlqasy vefrpsqgcs


61
pgyyrdhkgl ytgrcvpcnc nghsnqcqdg sgicvncqhn tagehcercq egyygnavhg


121
scracpcpht nsfatgcvvn ggdvrcscka gytgtqcerc apgyfgnpqk fggscqpcsc


181
nsngqlgsch pltgdcinqe pkdsspaeec ddcdscvmtl lndlatmgeq lrlvksqlqg


241
lsasaglleq mrhmetqakd lrnqllnyrs aisnhgskie glereltdln qefetlqeka


301
qvnsrkaqtl nnnvnratqs akeldvkikn virnvhillk qisgtdgegn nvpsgdfsre


361
waeaqrmmre lrnrnfgkhl reaeadkres qlllnrirtw qkthqgenng lansirdsln


421
eyeaklsdlr arlqeaaaqa kqanglnqen eralgaiqrq vkeinslqsd ftkylttads


481
sllqtnialq lmeksqkeye klaaslnear qelsdkvrel srsagktslv eeaekharsl


541
qelakqleei krnasgdelv rcavdaatay enilnaikaa edaanraasa sesalqtvik


601
edlprkaktl ssnsdkllne akmtqkklkq evspalnnlq qtlnivtvqk evidtnlttl


661
rdglhgiqrg didamissak smvrkandit devldglnpi qtdverikdt ygrtqnedfk


721
kaltdadnsv nkltnklpdl wrkiesinqq llplgnisdn mdrireliqq ardaaskvav


781
pmrfngksgv evrlpndled lkgytslslf lqrpnsreng gtenmfvmyl gnkdasrdyi


841
gmavvdgqlt cvynlgdrea elqvdqiltk setkeavmdr vkfqriyqfa rlnytkgats


901
skpetpgvyd mdgrnsntll nldpenvvfy vggyppdfkl psrlsfppyk gcielddlne


961
nvlslynfkk tfnlntteve pcrrrkeesd knyfegtgya rvptqphapi ptfgqtiqtt


1021
vdrgllffae ngdrfislni edgklmvryk lnselpkerg vgdainngrd hsiqikigkl


1081
qkrmwinvdv qntiidgevf dfstyylggi piairerfni stpafrgcmk nlkktsgvvr


1141
lndtvgvtkk csedwklvrs asfsrggqls ftdlglpptd hlqasfgfqt fqpsgilldh


1201
qtwtrnlqvt ledgyielst sdsgspifks pqtymdgllh yvsvisdnsg lrlliddqll


1261
rnskrlkhis ssrqslrlgg snfegcisnv fvqrlslspe vldltsnslk rdvslggcsl


1321
nkppflmllk gstrfnktkt frinqllqdt pvasprsvkv wqdacsplpk tqanhgalqf


1381
gdiptshllf klpqellkpr sqfavdmqtt ssrglvfhtg tknsfmalyl skgrlvfalg


1441
tdgkklriks kekcndgkwh tvvfghdgek grlvvdglra regslpgnst isirapvylg


1501
sppsgkpksl ptnsfvgclk nfqldskply tpsssfgvss clggplekgi yfseegghvv


1561
lahsvllgpe fklvfsirpr sltgilihig sqpgkhlcvy leagkvtasm dsgaggtsts


1621
vtpkqslcdg qwhsvavtik qhilhleldt dssytagqip fppastqepl hlggapanlt


1681
tlripvwksf fgclrnihvn hipvpvteal evqgpvslng cpdq


 







Protein sequence isoform 4 (SEQ ID NO (82)):








1
mppavrrsac smgwlwifga algqclgyss qqqrvpflqp pgqsqlqasy vefrpsqgcs


61
pgyyrdhkgl ytgrcvpcnc nghsnqcqdg sgicvncqhn tagehcercq egyygnavhg


121
scracpcpht nsfatgcvvn ggdvrcscka gytgtqcerc apgyfgnpqk fggscqpcsc


181
nsngqlgsch pltgdcinqe pkdsspaeec ddcdscvmtl lndlatmgeq lrlvksqlqg


241
lsasaglleq mrhmetqakd lrnqllnyrs aisnhgskie glereltdln qefetlqeka


301
qvnsrkaqtl nnnvnratqs akeldvkikn virnvhmlnr irtwqkthqg ennglansir


361
dslneyeakl sdlrarlqea aaqakqangl nqeneralga iqrqvkeins lqsdftkylt


421
tadssllqtn ialqlmeksq keyeklaasl nearqelsdk vrelsrsagk tslveeaekh


481
arslqelakq leeikrnasg delvrcavda atayenilna ikaaedaanr aasasesalq


541
tvikedlprk aktlssnsdk llneakmtqk klkqevspal nnlqqtlniv tvqkevidtn


601
lttlrdglhg iqrgdidami ssaksmvrka nditdevldg lnpiqtdver ikdtygrtqn


661
edfkkaltda dnsvnkltnk lpdlwrkies inqqllplgn isdnmdrire liqqardaas


721
kvavpmrfng ksgvevrlpn dledlkgyts lslflqrpns renggtenmf vmylgnkdas


781
rdyigmavvd gqltcvynlg dreaelqvdq iltksetkea vmdrvkfqri yqfarlnytk


841
gatsskpetp gvydmdgrns ntllnldpen vvfyvggypp dfklpsrlsf ppykgcield


901
dlnenvlsly nfkktfnlnt teveperrrk eesdknyfeg tgyarvptqp hapiptfgqt


961
iqttvdrgll ffaengdrfi slniedgklm vryklnselp kergvgdain ngrdhsiqik


1021
igklqkrmwi nvdvqntiid gevfdfstyy lggipiaire rfnistpafr gcmknlkkts


1081
gvvrlndtvg vtkkcsedwk lvrsasfsrg gqlsftdlgl pptdhlqasf gfqtfqpsgi


1141
lldhqtwtrn lqvtledgyi elstsdsgsp ifkspqtymd gllhyvsvis dnsglrllid


1201
dqllrmskrl khisssrqsl rlggsnfegc isnvfvqrls lspevldlts nslkrdvslg


1261
gcslnkppfl mllkgstrfn ktktfrinql lqdtpvaspr svkvwqdacs plpktqanhg


1321
alqfgdipts hllfklpqel lkprsqfavd mqttssrglv fhtgtknsfm alylskgrlv


1381
falgtdgkkl rikskekcnd gkwhtvvfgh dgekgrlvvd glraregslp gnstisirap


1441
vylgsppsgk pkslptnsfy gclknfqlds kplytpsssf gvssclggpl ekgiyfseeg


1501
ghvvlahsvl lgpefklvfs irprsltgil ihigsqpgkh lcvyleagkv tasmdsgagg


1561
tstsvtpkqs lcdgqwhsva vtikqhilhl eldtdssyta gqipfppast qeplhlggap


1621
anlttlripv wksffgclrn ihvnhipvpv tealevqgpv slngcpdq


 


Gene ID:
Y16


Gene symbol:
CD40


Gene description:
CD40 molecule


Unigene:
Hs.472860


Genbank:
AB209660


Entrez Gene:
958


Refseq:
NM_001250 | NM_152854







Protein sequence isoform 1 (SEQ ID NO (83)):








1
mvrlplqcvl wgclltavhp epptacrekq ylinsqccsl cqpgqklvsd cteftetecl


61
pcgesefldt wnrethchqh kycdpnlglr vqqkgtsetd tictceegwh ctseacescv


121
lhrscspgfg vkqiatgvsd ticepcpvgf fsnvssafek chpwtscetk dlvvqqagtn


181
ktdvvcgpqd rlralvvipi ifgilfaill vlvfikkvak kptnkaphpk qepqeinfpd


241
dlpgsntaap vqetlhgcqp vtqedgkesr isvqerq


 







Protein sequence isoform 2 (SEQ ID NO (84)):








1
mvrlplqcvl wgclltavhp epptacrekq ylinsqccsl cqpgqklvsd cteftetecl


61
pcgesefldt wnrethchqh kycdpnlglr vqqkgtsetd tictceegwh ctseacescv


121
lhrscspgfg vkqiatgvsd ticepcpvgf fsnvssafek chpwtrspgs aespggdphh


181
lrdpvchplg aglyqkggqe anq


 


Gene ID:
Y17


Gene symbol:
COL17A1


Gene description:
collagen, type XVII, alpha 1


Unigene:
Hs.117938


Genbank:
AL138761


Entrez Gene:
1308


Refseq:
NM_000494







Protein sequence (SEQ ID NO (85)):








1
mdvtkknkrd gtevterivt etvttrltsl ppkggtsngy aktaslgggs rlekqslthg


61
ssgyinstgs trghastssy rrahspastl pnspgstfer kthvtrhaye gsssgnsspe


121
yprkefasss trgrsqtres eirvrlqsas pstrwteldd vkrllkgsrs asysptrnss


181
ntlpipkkgt vetkivtass qsysgtydat ildanlpshv wsstlpagss mgtyhnnmtt


241
qsssllntna ysagsvfgvp nnmascsptl hpglstsssv fgmqnnlaps lttlshgttt


301
tstaygvkkn mpqspaavnt gvstsaactt svqsddllhk dckflilekd ntpakkemel


361
limtkdsgkv ftaspasiaa tsfsedtlkk ekqaaynads glkaeangdl ktvstkgktt


421
tadihsygss ggggsggggg vggagggpwg papawcpcgs ccswwkwllg llltwllllg


481
llfglialae evrklkarvd elerirrsil pygdsmdrie kdrlqgmapa agadldkigl


541
hsdsqeelwm fvrkklmmeq engnlrgspg pkgdmgspgp kgdrgfpgtp gipgplghpg


601
pqgpkgqkgs vgdpgmegpm gqrgregpmg prgeagppgs gekgergaag epgphgppgv


661
pgsvgpkgss gspgpqgppg pvglqglrge vglpgvkgdk gpmgppgpkg dqgekgprgl


721
tgepgmrglp gavgepgakg amgpagpdgh qgprgeqglt gmpgirgppg psgdpgkpgl


781
tgpqgpqglp gtpgrpgikg epgapgkivt segssmltvp gppgppgamg ppgppgapgp


841
agpaglpghq evlnlqgppg ppgprgppgp sipgppgprg ppgeglpgpp gppgsflsns


901
etflsgppgp pgppgpkgdq gppgprghqg eqglpgfsts gsssfglnlq gppgppgpqg


961
pkgdkgdpgv pgalgipsgp seggssstmy vsgppgppgp pgppgsisss gqeiqqyise


1021
ymqsdsirsy lsgvqgppgp pgppgpvtti tgetfdysel ashvvsylrt sgygvslfss


1081
sissedilav lqrddvrqyl rqylmgprgp pgppgasgdg sllsldyael ssrilsymss


1141
sgisiglpgp pgppglpgts yeellsllrg sefrgivgpp gppgppgipg nvwssisved


1201
lssylhtagl sfipgppgpp gppgprgppg vsgalatyaa ensdsfrsel isyltspdvr


1261
sfivgppgpp gpqgppgdsr llstdashsr gssssshsss vrrgssysss mstggggags


1321
lgaggafgea agdrgpygtd igpgggygaa aeggmyagng gllgadfagd ldynelavry


1381
sesmqrqgll qgmaytvqgp pgqpgpqgpp giskvfsays nvtadlmdff qtygaiqgpp


1441
gqkgemgtpg pkgdrgpagp pghpgppgpr ghkgekgdkg dqvyagrrrr rsiavkp


 


Gene ID:
Y18


Gene symbol:
DSC2


Gene description:
Desmocollin-2


Unigene:
Hs.95612


Genbank:
BC063291


Entrez Gene:
1824


Refseq:
NM_024422 | NM_004949







Protein sequence isoform Dsc2a (SEQ ID NO (86)):








1
meaarpsgsw ngalcrllll tlailifasd acknvtlhvp skldaeklvg rvnlkecfta


61
anlihssdpd fqiledgsvy ttntillsse krsftillsn tenqekkkif vflehqtkvl


121
kkrhtkekvl rrakrrwapi pcsmlenslg pfplflqqvq sdtaqnytiy ysirgpgvdq


181
eprnlfyver dtgnlyctrp vdreqyesfe iiafattpdg ytpelplpli ikiedendny


241
pifteetytf tifencrvgt tvgqvcatdk depdtmhtrl kysiigqvpp sptlfsmhpt


301
tgvitttssq ldrelidkyq lkikvqdmdg qyfglqttst ciiniddvnd hlptftrtsy


361
vtsveentvd veilrvtved kdlvntanwr anytilkgne ngnfkivtda ktnegvlcvv


421
kpinyeekqq milqigvvne apfsreaspr samstatvtv nvedqdegpe cnppiqtvrm


481
kenaevgtts ngykaydpet rsssgirykk ltdptgwvti dentgsikvf rsldreaeti


541
kngiynitvl asdqggrtct gtlgiilqdv ndnspfipkk tviickptms saeivavdpd


601
epihgppfdf slesstsevq rmwrlkaind taarlsyqnd ppfgsyvvpi tvrdrlgmss


661
vtsldvtlcd citendcthr vdpriggggv qlgkwailai llgiallfci lftlvcgasg


721
tskqpkvipd dlaqqnlivs nteapgddkv ysangfttqt vgasaqgvcg tvgsglkngg


781
qetiemvkgg hqtsescrga ghhhtldscr gghtevdncr ytysewhsft qprlgekvyl


841
cnqdenhkha qdyvltynye grgsvagsvg ccserqeedg lefldnlepk frtlaeacmk


901
r


 







Protein sequence isoform Dsc2b (SEQ ID NO (87)):








1
meaarpsgsw ngalcrllll tlailifasd acknvtlhvp skldaeklvg rvnlkecfta


61
anlihssdpd fqiledgsvy ttntillsse krsftillsn tenqekkkif vflehqtkvl


121
kkrhtkekvl rrakrrwapi pcsmlenslg pfplflqqvq sdtaqnytiy ysirgpgvdq


181
eprnlfyver dtgnlyctrp vdreqyesfe iiafattpdg ytpelplpli ikiedendny


241
pifteetytf tifencrvgt tvgqvcatdk depdtmhtrl kysiigqvpp sptlfsmhpt


301
tgvitttssq ldrelidkyq lkikvqdmdg qyfglqttst ciiniddvnd hlptftrtsy


361
vtsveentvd veilrvtved kdlvntanwr anytilkgne ngnfkivtda ktnegvlcvv


421
kplnyeekqq milqigvvne apfsreaspr samstatvtv nvedqdegpe cnppiqtvrm


481
kenaevgtts ngykaydpet rsssgirykk ltdptgwvti dentgsikvf rsldreaeti


541
kngiynitvl asdqggrtct gtlgiilqdv ndnspfipkk tviickptms saeivavdpd


601
epihgppfdf slesstsevq rmwrlkaind taarlsyqnd ppfgsyvvpi tvrdrlgmss


661
vtsldvticd citendcthr vdpriggggv qlgkwailai llgiallfci lftlvcgasg


721
tskqpkvipd dlaqqnlivs nteapgddkv ysangfttqt vgasaqgvcg tvgsgikngg


781
qetiemvkgg hqtsescrga ghhhtldscr gghtevdncr ytysewhsft qprlgeesir


841
ghtlikn


 


Gene ID:
Y19


Gene symbol:
DSC1


Gene description:
Desmocollin-1


Unigene:
Hs.567260


Genbank:
X72925


Entrez Gene:
1823


Refseq:
NM_024421lNM_004948







Protein sequence isoform Dsc1a (SEQ ID NO (88)):








1
malasaapgs ifckqllfsl lvltllcdac qkvylrvpsh lqaetlvgkv nleeclksas


61
lirssdpafr iledgsiytt hdlilsserk sfsiflsdgq rreqqeikvv lsarenkspk


121
krhtkdtalk rskrrwapip aslmenslgp fpqhvqqiqs daaqnytify sisgpgvdke


181
pfnlfyiekd tgdifctrsi drekyeqfal ygyattadgy apeyplphii kieddndnap


241
yfehrvtift vpencrsgts vgkvtatdld epdtlhtrlk ykilqqipdh pkhfsihpdt


301
gvittttpfl drekcdtyql imevrdmggq pfglfntgti tisledendn ppsftetsyv


361
teveenridv eilrmkvqdq dlpntphska vykilqgnen gnfiistdpn tnegvlcvvk


421
plnyevnrqv ilqvgvinea qfskaassqt ptmctttvtv kiidsdegpe chppvkviqs


481
qdgfpagqel lgykaldpei ssgeglryqk lgdednwfei nqhtgdlrtl kvldreskfv


541
knnqynisvv avdavgrsct gtlvvhlddy ndhapqidke vticqnnedf avlkpvdpdg


601
pengppfqff ldnsasknwn ieekdgktai lrqrqnldyn yysvpiqikd rhglvathml


661
tvrvcdcstp secrmkdkst rdvrpnvilg rwailamvlg svlllcilft cfcvtakrtv


721
kkcfpediaq qnlivsnteg pgeevteani rlpmqtsnic dtsmsvgtvg gqglktqqsf


781
emvkggytld snkggghqtl esvkgvgqgd tgryaytdwq sftqprlgek vylcgqdeeh


841
khcedyvcsy nyegkgslag svgccsdrqe eeglefldhl epkfrtlakt cikk


 







Protein sequence isoform Dsc1b (SEQ ID NO (89)):








1
malasaapgs ifckqllfsl lvltllcdac qkvylrvpsh lqaetlvgkv nleeclksas


61
lirssdpafr iledgsiytt hdlilsserk sfsiflsdgq rreqqeikvv lsarenkspk


121
krhtkdtalk rskrrwapip aslmenslgp fpqhvqqiqs daaqnytify sisgpgvdke


181
pfnlfyiekd tgdifctrsi drekyeqfal ygyattadgy apeyplplii kieddndnap


241
yfehrvtift vpencrsgts vgkvtatdld epdtlhtrlk ykilqqipdh pkhfsihpdt


301
gvittttpfl drekcdtyql imevrdmggq pfglfntgti tisledendn ppsftetsyv


361
teveenridv eilrmkvqdq dlpntphska vykilqgnen gnfiistdpn tnegvlcvvk


421
plnyevnrqv ilqvgvinea qfskaassqt ptmctttvtv kiidsdegpe chppvkviqs


481
qdgfpagqel lgykaldpei ssgeglryqk lgdednwfei nqhtgdlrtl kvldreskfv


541
knnqynisvv avdavgrsct gtlvvhlddy ndhapqidke vticqnnedf avlkpvdpdg


601
pengppfqff ldnsasknwn ieekdgktai lrqrqnldyn yysvpiqikd rhglvathml


661
tvrvcdcstp secrmkdkst rdvrpnvilg rwailamvlg svlllcilft cfcvtakrtv


721
kkcfpediaq qnlivsnteg pgeevteani rlpmqtsnic dtsmsvgtvg gqgiktqqsf


781
emvkggytld snkggghqtl esvkgvgqgd tgryaytdwq sftqprlgee sirghtlikn


 


Gene ID:
Y20


Gene symbol:
ITGA6


Gene description:
Integrin alpha-6


Unigene:
Hs.133397


Genbank:
X59512


Entrez Gene:
3655


Refseq:
NM_000210 | NM_001079818







Protein sequence isoform b (SEQ ID NO (90)):








1
maaagqlcll ylsagllsrl gaafnldtre dnvirkygdp gslfgfslam hwqlqpedkr


61
lllvgaprae alplqranrt gglyscdita rgpctriefd ndadptsesk edqwmgvtvq


121
sqgpggkvvt cahryekrqh vntkqesrdi fgrcyvlsqn lrieddmdgg dwsfcdgrlr


181
ghekfgscqq gvaatftkdf hyivfgapgt ynwkgivrve qknntffdmn ifedgpyevg


241
getehdeslv pvpansylgf sldsgkgivs kdeithtsga pranhsgavv llkrdmksah


301
llpehifdge glassfgydv avvdlnkdgw qdivigapqy fdrdgevgga vyvymnqqgr


361
wnnvkpirln gtkdsmfgia vknigdinqd gypdiavgap yddlgkvfiy hgsangintk


421
ptqvlkgisp yfgysiagnm dldrnsypdv avgslsdsvt ifrsrpvini qktitvtpnr


481
idlrqktacg apsgiclqvk scfeytanpa gynpsisivg tleaekerrk sglssrvqfr


541
nqgsepkytq eltlkrqkqk vcmeetlwlq dnirdklrpi pitasveiqe pssrrrvnsl


601
pevlpilnsd epktahidvh flkegcgddn vensnlkley kfctregnqd kfsylpiqkg


661
vpelvlkdqk dialeitvtn spsnprnptk dgddaheakl iatfpdtlty sayrelrafp


721
ekqlscvanq ngsqadcelg npfkrnsnvt fylvlsttev tfdtpdldin lklettsnqd


781
nlapitakak vvielllsvs gvakpsqvyf ggtvvgeqam ksedevgsli eyefrvinlg


841
kpltnlgtat lniqwpkeis ngkwllylvk veskglekvt cepqkeinsl nlteshnsrk


901
kreitekqid dnrkfslfae rkyqtlncsv nvncvnircp lrgldskasl ilrsrlwnst


961
fleeysklny ldilmrafid vtaaaenirl pnagtqvrvt vfpsktvaqy sgvpwwiilv


1021
ailagilmla llvfilwkcg ffkrnkkdhy datyhkaeih aqpsdkerlt sda


 







Protein sequence isoform a (SEQ ID NO (91)):








1
maaagqlcll ylsagllsrl gaafnldtre dnvirkygdp gslfgfslam hwqlqpedkr


61
lllvgaprae alplqranrt gglyscdita rgpctriefd ndadptsesk edqwmgvtvq


121
sqgpggkvvt cahryekrqh vntkqesrdi fgrcyvlsqn lrieddmdgg dwsfcdgrlr


181
ghekfgscqq gvaatftkdf hyivfgapgt ynwkgivrve qknntffdmn ifedgpyevg


241
getehdeslv pvpansylgf sldsgkgivs kdeitfvsga pranhsgavv llkrdmksah


301
llpehifdge glassfgydv avvdlnkdgw qdivigapqy fdrdgevgga vyvymnqqgr


361
wnnvkpirln gtkdsmfgia vknigdinqd gypdiavgap yddlgkvfiy hgsangintk


421
ptqvlkgisp yfgysiagnm dldrnsypdv avgslsdsvt ifrsrpvini qktitvtpnr


481
idlrqktacg apsgiclqvk scfeytanpa gynpsisivg tleaekerrk sglssrvqfr


541
nqgsepkytq eltlkrqkqk vcmeetlwlq dnirdklrpi pitasveiqe pssrrnvnsl


601
pevlpilnsd epktahidvh flkegcgddn vcnsnlkley kfctregnqd kfsylpiqkg


661
vpelvlkdqk dialeitvtn spsnprnptk dgddaheakl iatfpdtlty sayrelrafp


721
ekqlscvanq ngsqadcelg npfkrnsnvt fylvlsttev tfdtpdldin lklettsnqd


781
nlapitakak vvielllsys gvakpsqvyf ggtvvgeqam ksedevgsli eyefrvinlg


841
kpltnlgtat lniqwpkeis ngkwllylvk veskglekvt cepqkeinsl nlteshnsrk


901
kreitekqid dnrkfslfae rkyqtlncsv nvncvnircp lrgldskasl ilrsrlwnst


961
fleeysklny ldilmrafid vtaaaenirl pnagtqvrvt vfpsktvaqy sgvpwwiilv


1021
ailagilmla llvfilwkcg ffkrsrydds vpryhavrir keereikdek yidnlekkqw


1081
itkwnenesy s


 


Gene ID:
Y21


Gene symbol:
ITGB4


Gene description:
Integrin beta-4


Unigene:
Hs.632226


Genbank:
X53587 | X51841 | X52186


Entrez Gene:
3691


Refseq:
NM_000213 | NM_001005619 | NM_001005731







Protein sequence isoform 1 (SEQ ID NO (92)):








1
magprpspwa rlllaalisv slsgtlanrc kkapvkscte cvrvdkdcay ctdemfrdrr


61
cntqaellaa gcqresivvm essfqiteet qidttlrrsq mspqglrvrl rpgeerhfel


121
evfeplespv dlyilmdfsn smsddldnlk kmgqnlarvl sqltsdytig fgkfvdkvsv


181
pqtdmrpekl kepwpnsdpp fsfknvislt edvdefrnkl qgerisgnld apeggfdail


241
qtavctrdig wrpdsthllv fstesafhye adganvlagi msrnderchl dttgtytqyr


301
tqdypsvptl vrllakhnii pifavtnysy syyeklhtyf pvsslgvlqe dssnivelle


361
eafnrirsnl diraldsprg lrtevtskmf qktrtgsfhi rrgevgiyqv qlralehvdg


421
thvcqlpedq kgnihlkpsf sdglkmdagi icdvctcelq kevrsarcsf ngdfvcgqcv


481
csegwsgqtc ncstgslsdi qpclregedk pcsgrgecqc ghcvcygegr yegqfceydn


541
fqcprtsgfl cndrgrcsmg qcvcepgwtg pscdcplsna tcidsnggic ngrghcecgr


601
chchqqslyt dticeinysa ihpglcedlr scvqcqawgt gekkgrtcee cnfkvkmvde


661
lkraeevvvr csfrdedddc tysytmegdg apgpnstvlv hkkkdcppgs fwwlipllll


721
llpllallll lcwkycacck aclallpccn rghmvgfked hymlrenlma sdhldtpmlr


781
sgnlkgrdvv rwkvtnnmqr pgfathaasi nptelvpygl slrlarlcte nllkpdtrec


841
aqlrqeveen lnevyrqisg vhklqqtkfr qqpnagkkqd htivdtvlma prsakpallk


901
ltekqveqra fhdlkvapgy ytltadqdar gmvefqegve lvdvrvplfi rpedddekql


961
lveaidvpag tatlgrrlvn itiikeqard vvsfeqpefs vsrgdqvari pvirrvldgg


1021
ksqvsyrtqd gtaqgnrdyi pvegellfqp geawkelqvk llelqevdsl lrgrqvrrfh


1081
vqlsnpkfga hlgqphstti iirdpdeldr sftsqmlssq ppphgdlgap qnpnakaags


1141
rkihfnwlpp sgkpmgyrvk ywiqgdsese ahlldskvps veltnlypyc dyemkvcayg


1201
aqgegpyssl vscrthqevp sepgrlafnv vsstvtqlsw aepaetngei tayevcyglv


1261
nddnrpigpm kkvlvdnpkn rmllienlre sqpyrytvka rngagwgper eaiinlatqp


1321
krpmsipiip dipivdaqsg edydsflmys ddvlrspsgs qrpsvsddtg cgwkfepllg


1381
eeldlrrvtw rlppeliprl sassgrssda eaphgppddg gaggkggslp rsatpgppge


1441
hlvngrmdfa fpgstnslhr mtttsaaayg thlsphvphr vlstsstltr dynsltrseh


1501
shsttlprdy stltsvsshd srltagvpdt ptrlvfsalg ptslrvswqe prcerplqgy


1561
sveyqllngg elhrlnipnp aqtsvvvedl lpnhsyvfrv raqsqegwgr eregvities


1621
qvhpqsplcp 1pgsaftlst psapgplvft alspdslqls werprrpngd ivgylvtcem


1681
aqgggpataf rvdgdspesr ltvpglsenv pykfkvqart tegfgpereg iitiesqdgg


1741
pfpqlgsrag lfqhplqsey ssittthtsa tepflvdglt lgaqhleagg sltrhvtqef


1801
vsrtlttsgt lsthmdqqff qt


 







Protein sequence isoform 2 (SEQ ID NO (93)):








1
magprpspwa rlllaalisv slsgtlanrc kkapvkscte cvrvdkdcay ctdemfrdrr


61
cntqaellaa gcqresivvm essfqiteet qidttlrrsq mspqglrvil rpgeerhfel


121
evfeplespv dlyilmdfsn smsddldnlk kmgqnlarvl sqltsdytig fgkfvdkvsv


181
pqtdmrpekl kepwpnsdpp fsfknvislt edvdefrnkl qgerisgnld apeggfdail


241
qtavctrdig wrpdsthllv fstesafhye adganvlagi msrnderchl dttgtytqyr


301
tqdypsvptl vrllakhnii pifavtnysy syyeklhtyf pvsslgvlqe dssnivelle


361
eafnrirsnl diraldsprg lrtevtskmf qktrtgsfhi rrgevgiyqv qlralehvdg


421
thvcqlpedq kgnihlkpsf sdglkmdagi icdvctcelq kevrsarcsf ngdfvcgqcv


481
csegwsgqtc ncstgslsdi qpclregedk pcsgrgecqc ghcvcygegr yegqfceydn


541
fqcprtsgfl cndrgrcsmg qcvcepgwtg pscdcplsna tcidsnggic ngrghcecgr


601
chchqqslyt dticeinysa ihpglcedlr scvqcqawgt gekkgrtcee cnfkvkmvde


661
lkraeevvvr csfrdedddc tysytmegdg apgpnstvlv hkkkdcppgs fwwlipllll


721
llpllallll lcwkycacck aclallpccn rghmvgfked hymlrenlma sdhldtpmlr


781
sgnlkgrdvv rwkvtnnmqr pgfathaasi nptelvpygl slrlarlcte nllkpdtrec


841
aqlrqeveen lnevyrqisg vhklqqtkfr qqpnagkkqd htivdtvlma prsakpallk


901
ltekqveqra fhdlkvapgy ytltadqdar gmvefqegve lvdvrvplfi rpedddekql


961
lveaidvpag tatlgrrlvn itiikeqard vvsfeqpefs vsrgdqvari pvirrvldgg


1021
ksqvsyrtqd gtaqgnrdyi pvegellfqp geawkelqvk llelqevdsl lrgrqvrrfh


1081
vqlsnpkfga hlgqphstti iirdpdeldr sftsqmlssq ppphgdlgap qnpnakaags


1141
rkihfnwlpp sgkpmgyrvk ywiqgdsese ahlldskvps veltnlypyc dyemkvcayg


1201
aqgegpyssl vscrthqevp sepgrlafnv vsstvtqlsw aepaetngei tayevcyglv


1261
nddnrpigpm kkvlvdnpkn rmllienlre sqpyrytvka rngagwgper eaiinlatqp


1321
krpmsipiip dipivdaqsg edydsflmys ddvlrspsgs qrpsvsddte hlvngrmdfa


1381
fpgstnslhr mtttsaaayg thlsphvphr vlstsstltr dynsltrseh shsttlprdy


1441
stltsvsshg lppiwehgrs rlplswalgs rsraqmkgfp psrgprdsii lagrpaapsw


1501
gpdsrltagv pdtptrlvfs algptslrvs wqeprcerpl qgysveyqll nggelhrini


1561
pnpaqtsvvv edllpnhsyv frvraqsqeg wgreregvit iesqvhpqsp lcplpgsaft


1621
lstpsapgpl vftalspdsl qlswerprrp ngdivgylvt cemaqgggpa tafrvdgdsp


1681
esrltvpgls envpykfkvq arttegfgpe regiitiesq dggpfpqlgs raglfqhplq


1741
seyssittth tsatepflvd gltlgaqhle aggsltrhvt qefvsrtltt sgtlsthmdq


1801
qffqt


 







Protein sequence isoform 3 (SEQ ID NO (94)):








1
magprpspwa rlllaalisv slsgtlanrc kkapvkscte cvrvdkdcay ctdemfrdrr


61
cntqaellaa gcqresivvm essfqiteet qidttlrrsq mspqglrvrl rpgeerhfel


121
evfeplespv dlyilmdfsn smsddldnlk kmgqnlarvl sqltsdytig fgkfvdkvsv


181
pqtdmrpekl kepwpnsdpp fsfknvislt edvdefrnkl qgerisgnld apeggfdail


241
qtavctrdig wrpdsthllv fstesafhye adganvlagi msrnderchl dttgtytqyr


301
tqdypsvptl vrllakhnii pifavtnysy syyeklhtyf pvsslgvlqe dssnivelle


361
eafnrirsnl diraldsprg lrtevtskmf qktrtgsfhi rrgevgiyqv qlralehvdg


421
thvcqlpedq kgnihlkpsf sdglkmdagi icdvctcelq kevrsarcsf ngdfvcgqcv


481
csegwsgqtc ncstgslsdi qpclregedk pcsgrgecqc ghcvcygegr yegqfceydn


541
fqcprtsgfl cndrgrcsmg qcvcepgwtg pscdcplsna tcidsnggic ngrghcecgr


601
chchqqslyt dticeinysa ihpglcedlr scvqcqawgt gekkgrtcee cnfkvkmvde


661
lkraeevvvr csfrdedddc tysytmegdg apgpnstvlv hkkkdcppgs fwwlipllll


721
llpllallll lcwkycacck aclallpccn rghmvgfked hymlrenlma sdhldtpmlr


781
sgnlkgrdvv rwkvtnnmqr pgfathaasi nptelvpygl slrlarlcte nllkpdtrec


841
aqlrqeveen lnevyrqisg vhklqqtkfr qqpnagkkqd htivdtvlma prsakpallk


901
ltekqveqra fhdlkvapgy ytltadqdar gmvefqegve lvdvrvplfi rpedddekql


961
lveaidvpag tatlgrrlvn itiikeqard vvsfeqpefs vsrgdqvari pvirrvldgg


1021
ksqvsyrtqd gtaqgnrdyi pvegellfqp geawkelqvk llelqevdsl lrgrqvrrfh


1081
vqlsnpkfga hlgqphstti iirdpdeldr sftsqmlssq ppphgdlgap qnpnakaags


1141
rkihfnwlpp sgkpmgyrvk ywiqgdsese ahlldskvps veltnlypyc dyemkvcayg


1201
aqgegpyssl vscrthqevp sepgrlafnv vsstvtqlsw aepaetngei tayevcyglv


1261
nddnrpigpm kkvlvdnpkn rmllienlre sqpyrytvka rngagwgper eaiinlatqp


1321
krpmsipiip dipivdaqsg edydsflmys ddvlrspsgs qrpsysddte hlvngrmdfa


1381
fpgstnslhr mtttsaaayg thlsphvphr vlstsstltr dynsltrseh shsttlprdy


1441
stltsysshd srltagvpdt ptrlvfsalg ptslrvswqe prcerplqgy sveyqllngg


1501
elhrlnipnp aqtsvvvedl lpnhsyvfrv raqsqegwgr eregvities qvhpqsplcp


1561
lpgsaftlst psapgplvft alspdslqls werprrpngd ivgylvtcem aqgggpataf


1621
rvdgdspesr ltvpglsenv pykfkvqart tegfgpereg iitiesqdgg pfpqlgsrag


1681
lfqhplqsey ssittthtsa tepflvdglt lgaqhleagg sltrhvtqef vsrtlttsgt


1741
lsthmdqqff qt


 


Gene ID:
Y22


Gene symbol:
PVRL4


Gene description:
Poliovirus receptor-related protein 4


Unigene:
Hs.492490


Genbank:
BC010423


Entrez Gene:
81607


Refseq:
NM_030916







Protein sequence (SEQ ID NO (95)):








1
mplslgaemw gpeawlllll llasftgrcp ageletsdvv tvvlgqdakl pcfyrgdsge


61
qvgqvawarv dagegaqela llhskyglhv spayegrveq pppprnpldg svllrnavqa


121
degeyecrvs tfpagsfqar lrlrvlvppl pslnpgpale egqgltlaas ctaegspaps


181
vtwdtevkgt tssrsfkhsr saavtsefhl vpsrsmngqp ltcvvshpgl lqdqrithil


241
hvsflaeasv rgledqnlwh igregamlkc lsegqpppsy nwtrldgplp sgvrvdgdtl


301
gfpplttehs giyvchvsne fssrdsqvtv dvldpqedsg kqvdlvsasv vvvgviaall


361
fcllvvvvvl msryhrrkaq qmtqkyeeel tltrensirr lhshhtdprs qpeesvglra


421
eghpdslkdn sscsvmseep egrsystltt vreietqtel lspgsgraee eedqdegikq


481
amnhfvqeng tlrakptgng iyingrghlv


 


Gene ID:
Y23


Gene symbol:
SDC1


Gene description:
Syndecan-1


Unigene:
Hs.224607


Genbank:
BC008765


Entrez Gene:
6382


Refseq:
NM_001006946







Protein sequence (SEQ ID NO (96)):








1
mrraalwlwl calalslqpa lpqivatnlp pedqdgsgdd sdnfsgsgag alqditlsqq


61
tpstwkdtql ltaiptspep tgleataast stlpagegpk egeavvlpev epgltareqe


121
atprprettq lptthqastt tattaqepat shphrdmqpg hhetstpagp sqadlhtpht


181
edggpsater aaedgassql paaegsgeqd ftfetsgent avvavepdrr nqspvdqgat


241
gasqglldrk evlggviagg lvglifavel vgfmlyrmkk kdegsyslee pkqanggayq


301
kptkqeefya


 


Gene ID:
Z1


Gene symbol:
ENPP1


Gene description:
Ectonucleotide pyrophosphatase/ phosphodiesterase family member 1


Unigene:
Hs.527295


Genbank:
BC059375


Entrez Gene:
5167


Refseq:
NM_006208







Protein sequence (SEQ ID NO (97)):








1
merdgcaggg srggeggrap regpagngrd rgrshaaeap gdpqaaasll apmdvgeepl


61
ekaarartak dpntykvlsl vlsvcvltti lgcifglkps cakevksckg rcfertfgnc


121
rcdaacvelg nccldyqetc iepehiwtcn kfrcgekrlt rslcacsddc kdkgdcciny


181
ssvcqgeksw veepcesine pqcpagfetp ptllfsldgf raeylhtwgg llpvisklkk


241
cgtytknmrp vyptktfpnh ysivtglype shgiidnkmy dpkmnasfsl kskekfnpew


301
ykgepiwvta kyqglksgtf fwpgsdvein gifpdiykmy ngsvpfeeri lavlqwlqlp


361
kderphfytl yleepdssgh sygpvssevi kalqrvdgmv gmlmdglkel nlhrclnlil


421
isdhgmeqgs ckkyiylnky lgdvknikvi ygpaarlrps dvpdkyysfn yegiarnlsc


481
repnqhfkpy lkhflpkrlh faksdriepl tfyldpqwql alnpserkyc gsgfhgsdnv


541
fsnmqalfvg ygpgfkhgie adtfenievy nlmcdllnlt papnngthgs lnhllknpvy


601
tpkhpkevhp lvqcpftrnp rdnlgcscnp silpiedfqt qfnltvaeek iikhetlpyg


661
rprvlqkent icllsqhqfm sgysqdilmp lwtsytvdrn dsfstedfsn clyqdfripl


721
spvhkcsfyk nntkvsygfl sppqlnknss giysealltt nivpmyqsfq viwryfhdtl


781
lrkyaeerng vnvvsgpvfd fdydgrcdsl enlrqkrrvi rnqeilipth ffivltsckd


841
tsqtplhcen ldtlafilph rtdnsescvh gkhdsswvee llmlhrarit dvehitglsf


901
yqqrkepvsd ilklkthlpt fsqed


 


Gene ID:
Z2


Gene symbol:
CD34


Gene description:
Hematopoietic progenitor cell antigen CD34


Unigene:
Hs.374990


Genbank:
M81104


Entrez Gene:
947


Refseq:
NM_001773 | NM_001025109







Protein sequence isoform b (SEQ ID NO (98)):








1
mlvrrgarag prmprgwtal cllsllpsgf msldnngtat pelptqgtfs nvstnvsyqe


61
tttpstlgst slhpvsqhgn eattnitett vkftstsvit svygntnssv qsqtsvistv


121
fttpanvstp ettlkpslsp gnvsdlstts tslatsptkp ytssspilsd ikaeikcsgi


181
revkltqgic leqnktssca efkkdrgegl arvlcgeeqa dadagaqvcs lllaqsevrp


241
qclllvlanr teissklqlm kkhqsdlkkl gildfteqdv ashqsysqkt lialvtsgal


301
lavlgitgyf lmnrrswspt gerlelep


 







Protein sequence isoform a (SEQ ID NO (99)):








1
mlvrrgarag prmprgwtal cllsllpsgf msldnngtat pelptqgtfs nvstnvsyqe


61
tttpstlgst slhpvsqhgn eattnitett vkftstsvit svygntnssv qsqtsvistv


121
fttpanvstp ettlkpslsp gnvsdlstts tslatsptkp ytssspilsd ikaeikcsgi


181
revkltqgic leqnktssca efkkdrgegl arvlcgeeqa dadagaqvcs lllaqsevrp


241
qclllvlanr teissklqlm kkhqsdlkkl gildfteqdv ashqsysqkt lialvtsgal


301
lavlgitgyf lmnrrswspt gerlgedpyy tengggqgys sgpgtspeaq gkasvnrgaq


361
engtgqatsr nghsarqhvv adtel


 


Gene ID:
Z3


Gene symbol:
JAM3


Gene description:
Junctional adhesion molecule C


Unigene:
Hs.150718


Genbank:
BC012147


Entrez Gene:
83700


Refseq:
NM_032801 | NM_001205329







Protein sequence isoform 1 (SEQ ID NO (100)):








1
malrrpprlr lcarlpdffl lllfrgclig avnlkssnrt pvvqefesve lsciitdsqt


61
sdpriewkki qdeqttyvff dnkiqgdlag raeilgktsl kiwnvtrrds alyrcevvar


121
ndrkeideiv ieltvqvkpv tpvcrvpkav pvgkmatlhc qeseghprph yswyrndvpl


181
ptdsranprf rnssfhlnse tgtlvftavh kddsgqyyci asndagsarc eeqemevydl


241
niggiiggvl vvlavlalit lgiccayrrg yfinnkqdge syknpgkpdg vnyirtdeeg


301
dfrhkssfvi


 







Protein sequence isoform 2 (SEQ ID NO (101):








1
malrrpprlr lcarlpdffl lllfrgclig avnlkssnrt pvvqefesve lsciitdsqt


61
sdpriewkki qdeqttyvff dnkiqvkpvt pvcrvpkavp vgkmatlhcq eseghprphy


121
swyrndvplp tdsranprfr nssfhlnset gtlvftavhk ddsgqyycia sndagsarce


181
eqemevydln iggiiggvlv vlavlalitl giccayrrgy finnkqdges yknpgkpdgv


241
nyirtdeegd frhkssfvi


 


Gene ID:
Z4


Gene symbol:
CD14


Gene description:
Monocyte differentiation antigen CD14


Unigene:
Hs.163867


Genbank:
BC010507


Entrez Gene:
929


Refseq:
NM_000591







Protein sequence (SEQ ID NO (102)):








1
merascllll llplvhvsat tpepceldde dfrcvcnfse pqpdwseafq cvsaveveih


61
agglnlepfl krvdadadpr qyadtvkalr vrrltvgaaq vpaqllvgal rvlaysrlke


121
ltledlkitg tmpplpleat glalsslrlr nvswatgrsw laelqqwlkp glkvlsiaqa


181
hspafsceqv rafpaltsld lsdnpglger glmaalcphk fpaiqnlalr ntgmetptgv


241
caalaaagvq phsldlshns lratvnpsap rcmwssalns lnlsfagleq vpkglpaklr


301
vldlscnrln rapqpdelpe vdnltldgnp flvpgtalph egsmnsgvvp acarstlsvg


361
vsgtlvllqg argfa


 


Gene ID:
Z5


Gene symbol:
PLSCR4


Gene description:
Phospholipid scramblase 4


Unigene:
Hs.477869


Genbank:
AF199023


Entrez Gene:
57088


Refseq:
NM_001128304 | NM_001128306 | NM_001177304







Protein sequence isoform a (SEQ ID NO (103)):








1
msgvvptape qpagemenqt kppdprpdap peynshflpg ppgtavpppt gypgglpmgy


61
yspqqpstfp lyqpvggihp vryqpgkypm pnqsvpitwm pgptpmancp pgleylvqld


121
nihvlqhfep lemmtcfetn nrydiknnsd qmvyivtedt ddftrnayrt lrpfvlrvtd


181
cmgreimtmq rpfrctcccf ccpsarqele vqcppgvtig fvaehwnlcr avysiqnekk


241
envmrvrgpc stygcgsdsv fevksldgis nigsiirkwn gllsamadad hfdihfpldl


301
dvkmkamifg acflidfmyf ersppqrsr


 







Protein sequence isoform b (SEQ ID NO (104)):








1
msgvvptape qpagemenqt kppdprpdap peynshflpg ppgtavpppt gypgglpmgy


61
yspqqpstfp lyqpvggihp vryqpgkypm pnqsvpitwm pgptpmancp pgleylvqle


121
vqcppgvtig fvaehwnlcr avysiqnekk envmrvrgpc stygcgsdsv fevksldgis


181
nigsiirkwn gllsamadad hfdihfpldl dvkmkamifg acflidfmyf ersppqrsr


 







Protein sequence isoform c (SEQ ID NO (105)):








1
menqtkppdp rpdappeyns hflpgppgta vppptgypgg lpmgyyspqq pstfplyqpv


61
ggihpvryqp gkypmpnqsv pitwmpgptp mancppgley lvqlevqcpp gvtigfvaeh


121
wnlcravysi qnekkenvmr vrgpcstygc gsdsvfevks ldgisnigsi irkwngllsa


181
madadhfdih fpldldvkmk amifgacfli dfmyferspp qrsr


 


Gene ID:
Z6


Gene symbol:
AMOT


Gene description:
angiomotin


Unigene:
Hs.528051


Genbank:
AF286598


Entrez Gene:
154796


Refseq:
NM_133265 | NM_001113490







Protein sequence isoform 2 (SEQ ID NO (106)):








1
mpraqpssas yqpvpadpfa ivsraqqmve ilsdenrnlr qelegcyekv arlqkvetei


61
qrvseayenl vkssskreal ekamrnkleg eirrmhdfnr dlrerletan kqlaekeyeg


121
sedtrktisq lfaknkesqr ekekleaela tarstnedqr rhieirdqal snaqakvvkl


181
eeelkkkqvy vdkvekmqqa lvqlqaacek reqlehrlrt rlereleslr iqqrqgncqp


241
tnvseynaaa lmellrekee rilaleadmt kweqkyleen vmrhfaldaa atvaaqrdtt


301
vishspntsy dtaleariqk eeeeilmank rcldmegrik tlhaqiiekd amikvlqqrs


361
rkepskteql scmrpakslm sisnagsgll shsstltgsp imeekrddks wkgslgillg


421
gdyraeyvps tpspvppstp llsahsktgs rdcstqterg tesnktaava pisvpapvaa


481
aataaaitat aatitttmva aapvavaaaa apaaaaapsp ataaataaav spaaagqipa


541
aasvasaaav apsaaaaaav qvapaapapv papalvpvpa paaaqasapa qtqaptsapa


601
vaptpaptpt pavaqaevpa spatgpgphr lsipsltcnp dktdgpvfhs ntlerktpiq


661
ilgqepdaem veyli


 







Protein sequence isoform 1 (SEQ ID NO (107)):








1
mrnseeqpsg gttvlqrllq eqlrygnpse nrsllaihqq atgngppfps gsgnpgpqsd


61
vlspqdhhqq lvahaarqep qgqeiqsenl imekqlsprm qnneelptye eakvqsqyfr


121
gqqhasvgaa fyvtgvtnqk mrtegrpsvq rlnpgkmhqd eglrdlkqgh vrslserlmq


181
mslatsgvka hppvtsapls ppqpndlykn ptsssefyka qgplpnqhsl kgmehrgppp


241
eypfkgmppq svvckpqepg hfysehrlnq pgrtegqlmr yqhppeygaa rpaqdislpl


301
sarnsqphsp tssltsggsl pllqsppstr lsparhplvp nqgdhsahlp rpqqhflpnq


361
ahqgdhyrls qpglsqqqqq qqqqhhhhhh hqqqqqqqpq qqpgeaysam praqpssasy


421
qpvpadpfai vsraqqmvei lsdenrnlrq elegcyekva rlqkveteiq rvseayenlv


481
kssskreale kamrnklege irrmhdfnrd lrerletank qlaekeyegs edtrktisql


541
faknkesqre kekleaelat arstnedqrr hieirdqals naqakvvkle eelkkkqvyv


601
dkvekmqqal vqlqaacekr eqlehrlrtr lereleslri qqrqgncqpt nvseynaaal


661
mellrekeer ilaleadmtk weqkyleenv mrhfaldaaa tvaaqrdttv ishspntsyd


721
taleariqke eeeilmankr cldmegrikt lhaqiiekda mikvlqqrsr kepskteqls


781
cmrpakslms isnagsglls hsstltgspi meekrddksw kgslgillgg dyraeyvpst


841
pspvppstpl lsahsktgsr dcstqtergt esnktaavap isvpapvaaa ataaaitata


901
atitttmvaa apvavaaaaa paaaaapspa taaataaavs paaagqipaa asvasaaava


961
psaaaaaavq vapaapapvp apalvpvpap aaaqasapaq tqaptsapav aptpaptptp


1021
avaqaevpas patgpgphrl sipsltcnpd ktdgpvfhsn tlerktpiqi lgqepdaemv


1081
eyli


 


Gene ID:
Z7


Gene symbol:
ENPEP


Gene description:
glutamyl aminopeptidase (aminopeptidase A)


Unigene:
Hs.435765


Genbank:
L12468


Entrez Gene:
2028


Refseq:
NM_001977







Protein sequence (SEQ ID NO (108)):








1
mnfaeregsk ryciqtkhva ilcavvvgvg livglavglt rscdssgdgg pgtapapshl


61
psstaspsgp paqdqdicpa sedesgqwkn frlpdfvnpv hydlhvkpll eedtytgtvs


121
isinlsaptr ylwlhlretr itrlpelkrp sgdqvqvrrc feykkqeyvv veaeeeltps


181
sgdglylltm efagwlngsl vgfyrttyte ngqvksivat dheptdarks fpcfdepnkk


241
atytisithp keygalsnmp vakeesvddk wtrttfeksv pmstylvcfa vhqfdsvkri


301
snsgkpltiy vqpeqkhtae yaanitksvf dyfeeyfamn yslpkldkia ipdfgtgame


361
nwglityret nllydpkesa ssnqqrvatv vahelvhqwf gnivtmdwwe dlwlnegfas


421
ffeflgvnha etdwqmrdqm lledvlpvqe ddslmsshpi ivtvttpdei tsvfdgisys


481
kgssilrmle dwikpenfqk gcqmylekyq fknaktsdfw aaleeasrlp vkevmdtwtr


541
qmgypvlnvn gvknitqkrf lldpranpsq ppsdlgytwn ipvkwtedni tssvlfnrse


601
kegitlnssn psgnaflkin pdhigfyrvn yevatwdsia talslnhkif ssadraslid


661
dafalaraql ldykvalnlt kylkreenfl pwqrvisavt yiismfeddk elypmieeyf


721
qgqvkpiads lgwndagdhv tkllrssvlg fackmgdrea lnnasslfeq wlngtvslpv


781
nlrllvyryg mqnsgneisw nytleqyqkt slaqekekll yglasvknvt llsryldllk


841
dtnliktqdv ftviryisyn sygknmawnw iqlnwdylvn rytlnnrnlg rivtiaepfn


901
telqlwqmes ffakypqaga gekpreqvle tvknniewlk qhrntirewf fnllesg


 


Gene ID:
Z8


Gene symbol:
THY1


Gene description:
Thy-1 cell surface antigen


Unigene:
Hs.644697


Genbank:
AP003396


Entrez Gene:
7070


Refseq:
NM_006288







Protein sequence (SEQ ID NO (109)):








1
mnlaisiall ltvlqvsrgq kvtsltaclv dqslrldcrh entssspiqy efsltretkk


61
hvlfgtvgvp ehtyrsrtnf tskynmkvly lsaftskdeg tytcalhhsg hsppissqnv


121
tvlrdklvkc egisllaqnt swllllllsl sllqatdfms l

## Claims

1. A method of forming a composition enriched with human corneal endothelial cells comprising:
contacting a cell population containing human corneal endothelial cells expressing CD56 or CD166 with a first positive affinity reagent; and
selecting cells to which the first positive affinity reagent is bound,
wherein the first positive affinity reagent comprises an antibody that binds to CD56 surface protein expressed on the surface of human corneal endothelial cells, an antibody that binds to CD166 surface protein expressed on the surface of human corneal endothelial cells, an antibody that binds to coxsackievirus and adenovirus receptor (CAR) surface protein expressed on the surface of human corneal endothelial cells, or an antibody that binds to CD248 surface protein expressed on the surface of human corneal endothelial cells;
wherein the method comprises one or both of:
(i) the human corneal endothelial cells in the cell population express CD56, the first positive affinity reagent comprises an antibody or aptamer that binds to CD248 surface protein expressed on the surface of human corneal endothelial cells, an antibody or aptamer that binds to CAR surface protein expressed on the surface of human corneal endothelial cells, or an antibody or aptamer that binds to CD166 surface protein expressed on the surface of human corneal endothelial cells, and
(ii) further comprising:
contacting the cell population containing human corneal endothelial cells with a second positive affinity reagent; and
selecting cells to which the second positive affinity reagent is bound;
wherein the second positive affinity reagent differs from the first positive affinity reagent in regard to the identity of at least one surface protein that the first and second positive affinity reagents bind to and the second positive affinity reagent comprises an antibody or aptamer that binds to CD56 surface protein expressed on the surface of human corneal endothelial cells, an antibody or aptamer that binds to CD90 surface protein expressed on the surface of human corneal endothelial cells, an antibody or aptamer that binds to CD166 surface protein expressed on the surface of human corneal endothelial cells, an antibody or aptamer that binds to CD248 surface protein expressed on the surface of human corneal endothelial cells, or an antibody or aptamer that binds to CAR surface protein expressed on the surface of human corneal endothelial cells.

2. The method of claim 1, wherein the human corneal endothelial cells in the cell population express CD56, the first positive affinity reagent comprises an antibody or aptamer that binds to CD248 surface protein expressed on the surface of human corneal endothelial cells, an antibody or aptamer that binds to CAR surface protein expressed on the surface of human corneal endothelial cells, or an antibody or aptamer that binds to CD166 surface protein expressed on the surface of human corneal endothelial cells.

3. The method of claim 2, wherein said first positive affinity reagent comprises an antibody or aptamer that binds to CD166 surface protein expressed on the surface of human corneal endothelial cells.

4. The method of claim 1, further comprising:
contacting the cell population containing human corneal endothelial cells with a second positive affinity reagent; and
selecting cells to which the second positive affinity reagent is bound;
wherein the second positive affinity reagent compositionally differs from the first positive affinity reagent and the second positive affinity reagent comprises an antibody or aptamer that binds to CD56 surface protein expressed on the surface of human corneal endothelial cells, an antibody or aptamer that binds to CD90 surface protein expressed on the surface of human corneal endothelial cells, an antibody or aptamer that binds to CD166 surface protein expressed on the surface of human corneal endothelial cells, an antibody or aptamer that binds to CD248 surface protein expressed on the surface of human corneal endothelial cells, or an antibody or aptamer that binds to CAR surface protein expressed on the surface of human corneal endothelial cells.

5. The method of claim 1, further comprising:
contacting the cell population containing human corneal endothelial cells with a negative affinity reagent that selectively binds to human endothelial cells that have undergone a fibroblastic transformation relative to human corneal endothelial cells; and
removing cells that have undergone a fibroblastic transformation to which the negative affinity reagent is bound.

6. The method of claim 5, wherein the negative affinity reagent comprises an antibody or aptamer that binds to CD109 surface protein expressed on the surface of human corneal endothelial cells.

7. The method of claim 1, wherein the human corneal endothelial cells in the cell population express CD166, the first positive affinity reagent comprises an antibody or aptamer that binds to CD56 surface protein expressed on the surface of human corneal endothelial cells, an antibody or aptamer that binds to CD90 surface protein expressed on the surface of human corneal endothelial cells, an antibody or aptamer that binds to CD248 surface protein expressed on the surface of human corneal endothelial cells, or an antibody or aptamer that binds to CAR surface protein expressed on the surface of human corneal endothelial cells.

8. The method of claim 7, wherein the first positive affinity reagent comprises an antibody or aptamer that binds to CD56 surface protein expressed on the surface of human corneal endothelial cells, an antibody or aptamer that binds to CD248 surface protein expressed on the surface of human corneal endothelial cells, or an antibody or aptamer that binds to CAR surface protein expressed on the surface of human corneal endothelial cells.

9. The method of claim 7, wherein the first positive affinity reagent comprises an antibody or aptamer that binds to CD56 surface protein expressed on the surface of human corneal endothelial cells.

10. A composition enriched with human corneal endothelial cells comprising:
human corneal endothelial cells expressing at least one marker selected from the group consisting of: CD56, CD90, CD166, and coxsackie virus and adenovirus receptor (CAR); and
a first positive affinity reagent, wherein the first positive affinity reagent is an antibody against a surface protein expressed on the surface of human corneal endothelial cells, the surface protein selected from the group consisting of: CD56, CD90, CD166, CD248, and CAR,
wherein the marker expressed on the human corneal endothelial cells and the surface protein detected by the first positive affinity reagent are compositionally different;
wherein at least one of:
(i) the human corneal endothelial cell expresses CD56 and the first positive affinity reagent comprises an antibody or aptamer that binds to CD248 surface protein expressed on the surface of human corneal endothelial cells, an antibody or aptamer that binds to CAR surface proteins expressed on the surface of human corneal endothelial cells, an antibody or aptamer that binds to CD166 surface protein expressed on the surface of human corneal endothelial cells, or an antibody or aptamer that binds to CD90 surface protein expressed on the surface of human corneal endothelial cells and
(ii) the human corneal endothelial cell expresses CD56 and the first positive affinity reagent comprises an antibody or aptamer that binds to CD248 surface protein expressed on the surface of human corneal endothelial cells, an antibody or aptamer that binds to CAR surface protein expressed on the surface of human corneal endothelial cells, or an antibody or aptamer that binds to CD166 surface protein expressed on the surface of human corneal endothelial cells.

11. The composition of claim 10, wherein the human corneal endothelial cell expresses CD56 and the first positive affinity reagent comprises an antibody or aptamer that binds to CD248 surface protein expressed on the surface of human corneal endothelial cells, an antibody or aptamer that binds to CAR surface proteins expressed on the surface of human corneal endothelial cells, an antibody or aptamer that binds to CD166 surface protein expressed on the surface of human corneal endothelial cells, or an antibody or aptamer that binds to CD90 surface protein expressed on the surface of human corneal endothelial cells.

12. The composition of claim 11, wherein the first positive affinity reagent is an antibody or aptamer that binds to CD248 surface protein expressed on the surface of human corneal endothelial cells, an antibody or aptamer that binds to CAR surface protein expressed on the surface of human corneal endothelial cells, or an antibody or aptamer that binds to CD166 surface protein expressed on the surface of human corneal endothelial cells.

13. The composition of claim 12, wherein the first positive affinity reagent is an antibody or aptamer that binds to CD166 surface protein expressed on the surface of human corneal endothelial cells.

14. The composition of claim 10, wherein the composition further comprises a second positive affinity reagent that is compositionally different from the first positive affinity reagent, and the second positive affinity reagent is an antibody or aptamer that binds to CD90 surface protein expressed on the surface of human corneal endothelial cells, an antibody or aptamer that binds to CD166 surface protein expressed on the surface of human corneal endothelial cells, an antibody or aptamer that binds to CD248 surface protein expressed on the surface of human corneal endothelial cells, or an antibody or aptamer that binds to CAR surface protein expressed on the surface of human corneal endothelial cells.

15. The composition of claim 10, wherein the human corneal endothelial cells express CD166 and the first positive affinity reagent is an antibody or aptamer that binds to CD56 surface protein expressed on the surface of human corneal endothelial cells, an antibody or aptamer that binds to CD90 surface protein expressed on the surface of human corneal endothelial cells, an antibody or aptamer that binds to CD248 surface protein expressed on the surface of human corneal endothelial cells, or an antibody or aptamer that binds to CAR surface protein expressed on the surface of human corneal endothelial cells.

16. The composition of claim 15, wherein the first positive affinity reagent is an antibody or aptamer that binds to CD56 surface protein expressed on the surface of human corneal endothelial cells, an antibody or aptamer that binds to CD248 surface protein expressed on the surface of human corneal endothelial cells, or an antibody or aptamer that binds to CAR surface protein expressed on the surface of human corneal endothelial cells.

17. The composition of claim 15, wherein the first positive affinity reagent is an antibody or aptamer that binds to CD56 surface protein expressed on the surface of human corneal endothelial cells.

18. The composition of claim 10, wherein the composition further comprises a second positive affinity reagent that is compositionally different from the first positive affinity reagent, and the second positive affinity reagent is an antibody or aptamer that binds to CD56 surface protein expressed on the surface of human corneal endothelial cells, an antibody or aptamer that binds to CD90 surface protein expressed on the surface of human corneal endothelial cells, an antibody or aptamer that binds to CD248 surface protein expressed on the surface of human corneal endothelial cells, or an antibody or aptamer that binds to CAR surface protein expressed on the surface of human corneal endothelial cells.

19. The composition of claim 18, wherein the positive affinity reagents are coupled to a label.

20. The composition of claim 19, wherein the positive affinity reagents are coupled to different labels.