# Patent

## Title
2-decarboxy-2-phosphinico prostaglandin derivatives and methods for their preparation and use

## Abstract

2-Decarboxy-2-phosphinico prostaglandin derivatives are disclosed. These derivatives comprise a modified α-chain and an Ω-chain bonded to a ring structure. The modified α-chain has a 2-decarboxy-2-phosphinico group. The derivatives can be used to treat a variety of pharmaceutical and cosmetic conditions.

## Background
CROSS REFERENCE
This application is a divisional of U.S. application Ser. No. 09/633,180, filed Aug. 4. 2000, now U.S. Pat. No. 6,894,175 which claims priority under Title 35, United States Code §119(e) from Provisional Application Ser. No. 60/148,042, filed Aug. 4, 1999 and Provisional Application Ser. No. 60/158,637, filed Oct. 8, 1999.


FIELD OF THE INVENTION
This invention relates to prostaglandin derivatives and methods for their preparation and use. More specifically, this invention relates to 2-decarboxy-2-phosphinico prostaglandin derivatives and methods for their preparation and use.
BACKGROUND OF THE INVENTION
Prostaglandins are twenty carbon (C20) fatty acid derivatives. Prostaglandins bind with prostaglandin receptors. These receptors, eight of which have been reported to date, are members of the superfamily of seven transmembrane domain G-coupled receptors. Prostaglandin agonists activate the receptors to which they bind, and prostaglandin antagonists inhibit the receptors to which they bind; thereby producing biological effects.
For example, four receptors couple to an increase in intracellular cAMP, and are smooth muscle relaxants. EP4, DP, IP (prostacyclin) and EP2 are in this category. Three receptors couple to an increase in intracellular calcium and contract smooth muscle: the EP1 (see Funk C D, Furci L, Fitzgerald G A, Cloning and expression of a cDNA for the human prostaglandin receptor EP1 subtype. J. Biol. Chem. 1995 270, 18910–18916), the FP (ligand: PGF2α) (see Abramovitz M, Boie Y, Mguyen T, Rushmore T H, Bayne M A, Metters K M, Slipetz D M and Grygorczyk R Cloning and expression of a cDNA for the human prostanoid FP receptor. J. Biol. Chem 1994 269 2632–2636), and the TP (thromboxane) receptors. Finally, the EP3 receptor couples to a lowering of intracellular cAMP, and thus prevents relaxation of smooth muscle. Activation of this receptor blocks the forskolin-induced increase in intracellular cAMP. In addition to the gene products, alternate splicing gives rise to multiple isoforns of the EP3 (eight isoforms) (see Ichikawa E A Molecular aspects of the structures and functions of the prostaglandin E receptors J. Lipid Med. Cell Signal. 1996 14 83–87) and the TP (two isoforms) (see Krauss A H P, Woodward D F, Gibson L L, Protzman C E, Williams L S, Burk R M, Gac T S, Roof M B, Abbas F, Marshall K, Senior J Evidence for human thromboxane receptor heterogeneity using a novel series of 9,11-cyclic carbonate derivatives of prostaglandin-F2-alpha Br. J. Pharm. 1996 117(6) 1171–1180). These isoforms alter the carboxy-terminal region and thus the coupling of the G-proteins, rather than the prostaglandin binding regions. Pharmacological studies have provided evidence for the existence of other subtypes of receptors (see Corsini A. Folco G C, Fumagalli R. (5Z)-Carbacyclin discriminates between prostacyclin receptors coupled to adenylate cyclase in vascular smooth muscle and platelets. Br. J. Pharmacol. 1987 90, 255–261) or splice variants. The evidence is particularly compelling for the DP receptor and an FP or EP1 variant (Id., and see Woodward D F, Gil D W, Chen J, Burk R M, Kedzie, K M, Krauss, A H-P, Emerging evidence for additional prostanoid receptor subtypes Cur. Top. Pharmacol. 1998 4, 153–162; and Woodward D F, Madhu C, Rix P, Kharlamb A Studies on the ocular effects of a pharmacologically novel agent prostaglandin F2 alpha 1-OCH3 N-S Arch. Pharm., 1998. 358, (1). P1713—P1713). In addition, negative regulatory proteins (see Orlicky, D J Negative regulatory activity of a prostaglandin F2a receptor associated protein (FPRP) Prostaglandins, Leukotr. Ess. Fatty Acids 1996 54(5) 247–259; and Jakobsson P J, Morgenstern R, Mancini J, FordHutchinson A, Persson B Membrane-associated proteins in ecosanoid and glutathione metabolism (MAPEG)-A widespread protein superfamily Am. J. Resp. Crit. Care Med. 2000 161, S20–S24) and active transport proteins have recently been identified.
The receptors' nomenclature describes the naturally occurring prostaglandin to which they have the highest affinity, e.g., the EP series of receptors have the highest affinity for the ligand PGE2 (see Coleman R A, Smith, W L, Narumia S Classification of prostanoid receptors: properties, distribution, and structure of the receptors and their subtypes. Pharmacol. Rev. 1994 46, 205–229). However, all the naturally-occurring prostaglandins have some affinity for all eight of the receptors (see Kiriyama M, Ushikubi F, Kobayashi T, Hirata M, Sugimoto Y, Narumiya S Binding specificities of the prostanoid receptors. Br. J. Pharmacol. 1997 122 217–224).
Naturally-occurring prostaglandins include PGE1 and PGE2, PGF2α, prostacyclin (PGI), thromboxane and PGD2. Naturally occurring prostaglandins have substituent groups at the C9 and C11 positions on the cyclopentyl ring, a cis double bond between C5 and C6, and a trans double bond between C13 and C14. Thus, the naturally occurring prostaglandins are exemplified by the following structures.




All naturally occurring prostaglandins have a carboxylic acid moiety at the C1 position. The carboxylic acid moiety is a site for metabolic degradation by beta oxidation, which contributes to the rapid metabolism of the naturally occurring prostaglandins. Attempts have been made to prevent beta oxidation by modifying the carboxylic acid moiety at the 1 position as an ester moiety, a sulfonamide moiety, and as a tetrazole (see PCT Publication Nos. WO 99/12895, WO 99/12896, and WO 99/12898). However, such modifications have either resulted in only modest increases in half-life (such as the esters) or resulted in compounds with diminished potency.
An alternative approach has been to replace C1 with a heteroatom. For example, PGF analogs containing a sulfonic acid moiety at C1 (see Iguchi, Y.; Kori, S.; Hayashi, The chemistry of prostaglandins containing the sulfo group. M. J. Org. Chem., 1975 40, 521–523) and PGF analogs containing a phosphonic acid moiety at C1 (see Kluender, H. C. & Woessner, W., The Synthesis of dimethylphosphonoprostaglandin analogs, Prostaglandins and Medicine, 1979 2: pp.441–444,) have been disclosed. However, such compounds suffer from significantly diminished potency.
Further research in the area of heteroatom-containing C1 replacements has been hampered by the lack of a general synthetic route to advanced or key intermediates that would allow for the rapid preparation of a multitude of variants to replace C1. The Corey route to prostaglandins was specifically designed for a carboxcyclic acid moiety, and modifications which create reagents with relatively acidic protons are either incompatible with this route or cause significant optimization of this difficult step for each new C1 replacement. Syntheses of Prostaglandin analogs via the Corey route are described in the following references: Corey, E. J.; Weinshenker, N. M.; Schaaf, T. K.; Huber, W J. Am. Chem. Soc., 1969, 91, 5675 and Corey, E. J.; Schaaf, T. K.; Huber, W; Koelliker, U.; Weinshenker, N. M.; J. Am. Chem. Soc., 1970, 92, 397.
Thus, while a few prostaglandin analogs wherein C1 has been replaced with a heteroatom-containing moiety have been disclosed, there is a continuing need for suitable C1 replacements that result in potent, selective prostaglandin derivatives for the treatment of a variety of diseases and other conditions. Therefore, it is an object of this invention to provide 2-decarboxy-2-phosphinico derivatives of prostaglandins that can be used to treat medical and cosmetic conditions.
Prostaglandin analogs have potent biological activities of a hormonal or regulatory nature. Examples of the biological activities and the conditions they can be used to treat are discussed below.
PGE Analogs
Analogs of PGE are useful for treating a variety of medical conditions including pain. For example, EP1 receptor antagonists have been used to block the pain induced by PGE2 injections in mice, because of their affinities for the EP1 receptor (see Ruel R, Lacombe P, Abramovitz M, Godbout C, Lamontagne S, Rochette C, Sawyer N, Stocco R, Tremblay N M, Metters K M, Labelle M New class of biphenylene dibenzazocinones as potent ligands for the human EP1 prostanoid receptor Bioorg. Med. Chem. Lett. 1999 9 2699–2704; and Hallinan E A, Hagen T J, Tsymbalov S, Husa R K, Lee A C, Staplefield A, Savage M A Aminoacetyl moiety as a potential surrogate for diacylhydrazine group of SC-51089, a potent PGE2 antagonist, and its analogs J. Med. Chem. 1996 39 609).
Another use for PGE analogs is the treatment of arthritis. As opposed to using an EP1 antagonist to prevent pain as a treatment for arthritis, an alternate approach for the attenuation of the disease is by activating chondrocytes with a prostaglandin that activates the EP3 receptor, and possibly the EP4 receptor (see Kiriyama M, Ushikubi F, Kobayashi T, Hirata M, Sugimoto Y, Narumiya S Binding specificities of the prostanoid receptors. Br. J. Pharmacol. 1997 122 217–224; and Narumiya S Roles of prostanoids in health and disease, lessons from receptor-knockout mice Int. Congr. Ser. 1999 1181 261–269).
PGE analogs are also useful to treat bone disorders such as osteoporosis. Bone stimulating properties of prostaglandins have been noticed in infants being dosed with PGE1 as a part of treatment for patent ductus arteriosis (see Ueda K, Saito A, Nakano H, Aoshima M, Yokota M. Muraoka R, Iwaya T Brief clinical and laboratory observations. Cortical hyperostosis following long-term administration of prostaglandin E1 in infants with cyanotic congenital heart disease. J. Pediat. 1980, 97, 834). Systemically administered PGE2 was subsequently found to stimulate new bone formation in dogs (see Shih M S, Norridin R W PGE2 induces regional remodeling changes in Haversian envelope: A histomorphometric study of fractured ribs in beagles Bone and Mineral 1986 1 227), in estrogen-depleted rats (see Mori S, Jee W S S, Li X J, Chan S, Kimmel D B Effects of prostaglandin E2 on production of new cancellous bone in the axial skeleton of ovariectomized rats. Bone 1990 11 103) and to stimulate remodeling in the proximal tibia of estrogen-depleted rats (see Chyun Y S, Raisz L G Stimulation of bone formation by prostaglandin E2 Prostaglandins 1984 27 97). PGE2 strongly stimulates bone resorption as well as bone formation. However, it is thought that more than one receptor may be responsible for the bone anabolic effects of PGE2 (see Roof S L, deLong M A, Charest R P Messenger-RNA expression of prostaglandin receptors EP1, EP2, EP3 and EP4 in human osteoblast-like cells and 23 human tissues. J. Bone Min. Res., 1996 11, S337; Maruyama, T, Ohuchida S New 3,7 dithiaprostanoic acid derivatives useful for the prevention and treatment of abnormal bone formation and neuronal cell death. EP 855389, 29 Jul. 1998; Sakuma Y, Tanaka K, Suda M, Yasoda A, Natsui K, Tanaka I, Ushikubi F, Narumiya S, Segi E, Sugimoto Y, Ichikawa A, Nakao K Crucial involvement of the EP4 subtype of prostaglandin E receptor in osteoclast formation by proinflammatory cytokines and lipopolysaccharide J. Bone Miner. Res. 2000 15(2) 218–227).
PGE derivatives may also be used to treat vascular disease. PGE1 is used clinically to lower blood pressure and improve vascular circulation. The role that the EP receptors have in the vasculature is now being determined. In a recent report of murine prostanoid receptor knockout (KO) mice, there was demonstrated a sexual dimorphism in EP-mediated blood pressure regulation (see Audoly L P, Tilley J, Key M, Nguyen M, Stock J L, McNeish J D, Koller B H, Coffman T M Identification of specific EP receptors responsible for the hemodynamic effects of PGE2 Am. J. Physiol. 1999 46(3) H924–930). In female KO mice, the EP2 and EP4 receptors mediated a vasodepressor response, whilst in the males it is the EP1 receptor that mediates the vasodepressor response, which is opposed by the EP3 receptor.
Additionally, antagonists of prostaglandin E2 receptors, particularly EP4 receptors have a diuretic effect and may be used for treating hypertension and premenstrual tension), protecting agents for nerve cells, lowering intraocular pressure for treating glaucoma (see EP2 selective agonists, and selective agonists of the EP1 receptor were similarly effective at lowering intraocular pressure. However, the EP1 selective agonists may cause pain as a side effect.
PGE analogs can be used to sexual dysfunction such as erectile dysfunction (see Maw G N Pharmacological therapy for the treatment of erectile dysfunction Annu. Rep. Med. Chem. 1999 34 71–80; and Anon. Alprostadil (nexmed): Alprox-TD, Befar, Femprox, prostaglandin E1 (nexmed) Drugs R&D 1999, 2(6) 413–414) and to treat women's sexual arousal dysfunction).
PGE analogs can be used to treat asthma (see Karim S M M, Adaikin P G, Kottegoda S R Prostaglandins and human respiratory tract smooth muscle: Structure activity relationship. Adv. Prostaglandin Thromboxane Res. 1980 7 969–980). To date, asthma treatment with prostaglandins has focused on the relaxant EP2 and EP4 receptors and thromboxane antagonists.
PGE analogs can be used for enhancing skin pigmentation. For example, PGE1 can be used in conjunction with phosphodiesterase inhibitors to enhance skin pigmentation. The enhancement of activity in the presence of diesterase inhibitors suggests that the EP2 and the EP4 receptors may be responsible.
PGE analogs, such as those having affinity for the EP2 and EP4 receptors can be used to inhibit cell migration.
Furthermore, EP3 selective agonists can be used to enhance the uterine contractions of PGF2α, and inhibit gastric acid secretion.
EP3-selective ligands may useful in preventing or treating, or both, such conditions as hepatic diseases, renal diseases, pancreatitis, and myocardial infarct (see Flisiak R, Prokopowicz D Effect of misoprostol on the course of viral hepatitis B Hepato-Gastroenterology 1997 44(17), 1419–1425; and Mihele, Denisa; Cristea, Elena; Mihele, Dana; Cocu, F. The testing of the hepatoprotective action of some new synthetic prostaglandins Farmacia (Bucharest) 1999 47(5), 43–58; and Vengerovsky A I, Baturina N O, Saratikov A S Hepatoprotective action of prostaglandins Eksp. Klin. Farmakol. 1997 60(5), 78–82).
The EP4 selective ligands have also been used for the prevention of neuronal cell death. EP2 selective ligands can be used for protection against neuronal damage in the eye. Therefore, it is an object of this invention to provide novel prostaglandin derivatives that can be used as protective agents for nerve cells.
PGF Analogs
Analogs of PGF2a are also useful for the treatment of a variety of medical conditions. For example, PGF analogs can be used to treat bone disorders, such as osteoporosis. For example, one approach is to selectively activate the excitatory FP receptor as a means of reversing osteoporosis (see Hartke, J R, Jankowsky, M L, deLong M A, Soehner M E, Jee W S S, Lundy M W Prostanoid F P agonists build bone in the ovariectomized rat. J. Bone Min. Res. 1999, 14, S207; and Lundy M W, deLong M A, Combs K S, Gross G J, Soehner M E, Hartke J R Restoration of cancellous architecture and increased bone strength in aged osteopenic rats treated with fluprostenol J. Bone Min. Res. 1999 14, S401.29).
FP ligands have also been proposed for the management of vascular diseases e.g., as vasorelaxants. It is likely that the activity of these ligands is mediated by a distinct subtype or splice variant of the FP receptor. PGF1 analogs have also been disclosed for use in the treatment of diabetic and other forms of peripheral vascular disease (see Ueno, R Ueno, R, Oda, T. Prostaglandins of the F series U.S. Pat. No. 5,770,759. Jun. 23 1998), perhaps mediated by the same receptor.)
FP receptor ligands may be used to treat ocular disorders such as glaucoma (see Kerstetter J R, Brubaker R F, Wilson S E, Kullerstrand L J Prostaglandin F2 alpha-1-isopropylester lowers intraocular pressure without decreasing aqueous humor flow Am. J. Ophth. 1988 105 30–34).
PGF analogs can be used as sleep inducing agents (see Matsumura H Prostaglandins and sleep Saishin No to Shinkei Kagaku Shirizu 1998 10 79–89).
Other uses for the PGF derivatives include treating skin disorders; circulatory disorders, such as hypertension; gastrointestinal disorders; hair loss; respiratory disorders; and fertility control. More information regarding the biological effects of Prostaglandin F analogs is disclosed in the following references: PCT Publication No. WO 99/12895, 1999; PCT Publication No. WO 99/12896, 1999; PCT Publication No. WO 99/12898; Abstr. 1999, 194116 Molecular mechanisms of diverse actions of prostanoid receptors, Biochimica et Biophysica Acta, 1259 (1995) 109–120; U.S. Pat. Nos. 3,776,938 and 3,882,241; G.B. Patent No. 1,456,512 (1976) issued to Pfizer Inc., Bundy, G. L.; Lincoln, F. H., Synthesis of 17-Phenyl-18,19,20-trinor prostaglandins I. The PG1 Series Prostaglandins 1975 9 1–4; CRC Handbook of Eicosanoids: Prostaglandins and Related Lipids Vol. 1, Chemical and Biochemical Aspects, Parts A & B, A. L. Willis, eds., CRC Press (1987); Liljebris, C.; et. al. Derivatives of 17-Phenyl-18,19,20-trinorprostaglandin F2a Isopropyl Ester: Potential Antiglaucoma Agents, Journal of Medicinal Chemistry 1995 38 289–304; Collins, P. W.; Djuric, S. W. Synthesis of Therapeutically Useful Prostaglandin and Prostacyclin Analogs, Chemical Reviews 1993 93 1533–1564.
PGI Analogs
PGI analogs of prostaglandins have been disclosed for use in the reproductive system (see Negishi, M., Sugimoto, Y., Ichikawa, A. Molecular mechanisms of the diverse actions of prostanoid receptors. Biochim. Biophys. Acta 1259 1995 109–120; and Collins P W, Djuric S W Synthesis of therapeutically useful prostaglandin and prostacyclin analogs Chem. Rev. 1993 93 1533–1564). PGI derivatives function as cervical maturing agents and can be used to inhibit uterine contraction and prevent premature delivery. Prostacyclin (PGI2) is also used clinically to lower blood pressure and improve vascular circulation.
IP ligands have also been proposed for this area, e.g., treating peripheral vascular disease, treating pulmonary hypertension, and reducing blood pressure (see Vayssairat M Preventive effect of an oral prostacyclin analog, beraprost sodium, on digital necrosis in systemic sclerosis J. Rheumatol. 1999 26(10) 2173–2178; Murakami T, Sawada K Effect of isocarbacyclin methyl ester incorporated in lipid microspheres on experimental models of peripheral obstructive disease. Arzn. Forschung. 1995 45 991–994: and Hall A, Smith W H T Clinprost Teijin Curr. Opin. Cardiovasc; Pulm. Renal Invest. Drugs 1999 1(5) 605–610).
Prostaglandins have been proposed as protective agents for nerve cells. Several receptors several receptors have been investigated, with the ‘relaxant’ receptors being preferred.
PGD Analogs
PGD analogs can be used as sleep inducing agents. Sleep induction is generally thought to arise as a result of stimulation of the DP receptor (see Matsumura H Prostaglandins and sleep Saishin No to Shinkei Kagaku Shirizu 1998 10 79–89). Antagonists of the DP receptor, may be orally active and may be used as anti-allergy agents.
Thromboxanes
Some thromboxane analogs are orally active and may be used as anti-allergy agents. Thromboxane analogs may also be used to treat vascular disease; thromboxane antagonists are used to prevent platelet coagulation.
Thromboxane mediates potent vasoconstriction and irreversible platelet aggregation (see Terada N, Yamakoshi T, Hasegawa M, Tanikawa H, Nagata H, Maesako K I, Konno A Effect of a thromboxane A2 receptor antagonist, ramatroban (BAY u3405), on inflammatory cells, chemical mediators and non-specific nasal hyperreactivity after allergen challenge in patients with perennial allergic rhinitis. Allergol. Int. 1998 47(1), 59–67; and Miyamoto T, Takishima T A comparison in the efficacy and safety between ramatroban (BAY u 3405) and ozagrel-HCl for bronchial asthma: a phase III, multi-center, randomized, double-blind, group comparative study. Rinsho Iyaku. 1997 13 599–639). Thromboxane synthesis inhibitors have also been proposed for these applications (see McCullough P A Ridogrel (Janssen) Curr. Opin. Anti-Inflammatory Immunomodulatory Invest. Drugs 1999 1(3), 265–276; Inoue H Thromboxane A2 receptor antagonists Farumashia 1996, 32(10), 1221–1225; Cayatte A J, Du Y The thromboxane A2 receptor antagonist, S18886, decreases atherosclerotic lesions and serum intracellular adhesion molecule-1 in the Apo E knockout mouse. Circulation. 1998 98 115; Verbeuren T, Descombes J J The TP-receptor antagonist S 18886 unmasks vascular relaxation and potentiates the anti-platelet action of PGD2. Thromb. Haemostasis. 1997 693; and Yoshida K, Sato H Synthesis and pharmacological activities of the new TXA2 receptor antagonist Z-335 and related compounds. AFMC 1995 95 53).
Other Prostaglandins
Other prostaglandins, such as PGA1 in conjunction with phosphodiesterase inhibitors, may be used to enhance skin pigmentation. The enhancement of activity in the presence of diesterase inhibitors suggests that the EP2 and the EP4 receptors may be responsible.
Alzheimer's Disease and renal salt wasting may be controlled by inhibition of the synthesis or activity of delta-12-PGJ2. This prostaglandin is a known ligand of PPARγ.
However, none of the PGE, PGD, thromboxane, and PGI analogs disclosed above have a 2-decarboxy-2-phosphinico group at C1. Therefore, it is an object of this invention to provide prostaglandin derivatives wherein C1 has been replaced with a 2-decarboxy-2-phosphinico group. Furthermore, prostaglandins having a carboxyl group at C1 suffer from the drawback that they are rapidly metabolized and excreted. It is a further object of this invention to provide prostaglandin derivatives that are less rapidly metabolized and excreted than known prostaglandins and derivatives thereof.
SUMMARY OF THE INVENTION
This invention relates to 2-decarboxy-2-phosphinico prostaglandin derivatives and methods for their preparation and use. These derivatives comprise a modified α-chain and an Ω-chain bonded to a ring structure. The modified α-chain has a 2-decarboxy-2-phosphinico group at position C1. Without wishing to be bound by theory, it is thought that the reason prior art prostaglandins are rapidly metabolized and excreted is because transport proteins in a subject's kidneys bind with the carboxyl group at C1 and actively metabolize and excrete the prostaglandins. It is further theorized that these transport proteins do not recognize, or at least do not as readily bind with, the 2-decarboxy-2-phosphinico group at position C1, thereby rendering the 2-decarboxy-2-phosphinico prostaglandin derivatives longer lasting.


DETAILED DESCRIPTION OF THE INVENTION
Publications and patents are referred to throughout this disclosure. All U.S. Patents cited herein are hereby incorporated by reference.
All percentages, ratios, and proportions used herein are by weight unless otherwise specified.
Definition and Usage of Terms
The following is a list of definitions for terms, as used herein:
“Activate” means binding and signal transduction of a receptor.
“Acyl group” means a monovalent group suitable for acylating a nitrogen atom to form an amide or carbamate, an alcohol to form a carbonate, or an oxygen atom to form an ester group. Preferred acyl groups include benzoyl, acetyl, tert-butyl acetyl, para-phenyl benzoyl, and trifluoroacetyl. More preferred acyl groups include acetyl and benzoyl. The most preferred acyl group is acetyl.
“Agonist” means a compound that activates a receptor.
“Antagonist” means a compound that inhibits a receptor.
“Aromatic group” means a monovalent group having a monocyclic ring structure or fused bicyclic ring structure. Monocyclic aromatic groups contain 5 to 10 carbon atoms, preferably 5 to 7 carbon atoms, and more preferably 5 to 6 carbon atoms in the ring. Bicyclic aromatic groups contain 8 to 12 carbon atoms, preferably 9 or 10 carbon atoms in the ring. Bicyclic aromatic groups include groups wherein only one ring is aromatic. Aromatic groups are unsubstituted. The most preferred aromatic group is phenyl.
“Carbocyclic group” means a monovalent saturated or unsaturated hydrocarbon ring. Carbocyclic groups are monocyclic, or are fused, spiro, or bridged bicyclic ring systems. Monocyclic carbocyclic groups contain 4 to 10 carbon atoms, preferably 4 to 7 carbon atoms, and more preferably 5 to 6 carbon atoms in the ring. Bicyclic carbocyclic groups contain 8 to 12 carbon atoms, preferably 9 to 10 carbon atoms in the ring. Carbocyclic groups are unsubstituted. Preferred carbocyclic groups include cyclopentyl, cyclohexyl, cyclohexenyl, cycloheptyl, and cyclooctyl. More preferred carbocyclic groups include cyclohexyl, cycloheptyl, and cyclooctyl. The most preferred carbocyclic group is cycloheptyl. Carbocyclic groups are not aromatic.
“DP receptor” means known human DP receptors, their splice variants, and undescribed receptors that have similar binding and activation profiles as the known human DP receptors.
“EP receptor” means known human EP receptors, their splice variants, and undescribed receptors that have similar binding and activation profiles as the known human EP receptors.
“FP receptor” means known human FP receptors, their splice variants, and undescribed receptors that have similar binding and activation profiles as the known human FP receptors.
“Halogen atom” means F, Cl, Br, or I. Preferably, the halogen atom is F, Cl, or Br; more preferably Cl or F; and most preferably F.
“Halogenated hydrocarbon group” means a substituted monovalent hydrocarbon group or a substituted carbocyclic group, wherein at least one substituent is a halogen atom. Halogenated hydrocarbon groups can have a straight, branched, or cyclic structure. Preferred halogenated hydrocarbon groups have 1 to 12 carbon atoms, more preferably 1 to 6 carbon atoms, and most preferably 1 to 3 carbon atoms. Preferred halogen atom substituents are Cl and F. The most preferred halogenated hydrocarbon group is trifluoromethyl.
“Heteroaromatic group” means an aromatic ring containing carbon and 1 to 4 heteroatoms in the ring. Heteroaromatic groups are monocyclic or fused bicyclic rings. Monocyclic heteroaromatic groups contain 5 to 10 member atoms (i.e., carbon and heteroatoms), preferably 5 to 7, and more preferably 5 to 6 in the ring. Bicyclic heteroaromatic rings contain 8 to 12 member atoms, preferably 9 or 10 in the ring. Heteroaromatic groups are unsubstituted. Preferred heteroaromatic groups include thienyl, thiazolyl, purinyl, pyrimidyl, pyridyl, and furanyl. More preferred heteroaromatic groups include thienyl, furanyl, and pyridyl. The most preferred heteroaromatic group is thienyl.
“Heteroatom” means an atom other than carbon in the ring of a heterocyclic group or the chain of a heterogeneous group. Preferably, heteroatoms are selected from the group consisting of nitrogen, sulfur, and oxygen atoms. Groups containing more than one heteroatom may contain different heteroatoms.
“Heterocyclic group” means a saturated or unsaturated ring structure containing carbon and 1 to 4 heteroatoms in the ring. No two heteroatoms are adjacent in the ring. Heterocyclic groups are not aromatic. Heterocyclic groups are monocyclic, or are fused or bridged bicyclic ring systems. Monocyclic heterocyclic groups contain 4 to 10 member atoms (i.e., including both carbon atoms and at least 1 heteroatom), preferably 4 to 7, and more preferably 5 to 6 in the ring. Bicyclic heterocyclic groups contain 8 to 12 member atoms, preferably 9 or 10 in the ring. Heterocyclic groups are unsubstituted. Preferred heterocyclic groups include piperzyl, morpholinyl, tetrahydrofuranyl, tetrahydropyranyl, and piperdyl.
“Heterogeneous group” means a saturated or unsaturated chain containing 1 to 18 member atoms (i.e., including both carbon and at least one heteroatom). No two heteroatoms are adjacent. Preferably, the chain contains 1 to 12 member atoms, more preferably 1 to 6, and most preferably 1 to 4. The chain may be straight or branched. Preferred branched heterogeneous groups have one or two branches, preferably one branch. Preferred heterogeneous groups are saturated. Unsaturated heterogeneous groups have one or more double bonds, one or more triple bonds, or both. Preferred unsaturated heterogeneous groups have one or two double bonds or one triple bond. More preferably, the unsaturated heterogeneous group has one double bond. Heterogeneous groups are unsubstituted.
“P receptor” means known human IP receptors, their splice variants, and undescribed receptors that have similar binding and activation profiles as the known human IP receptors.
“Monovalent hydrocarbon group” means a chain of 1 to 18 carbon atoms, preferably 1 to 12, more preferably 1 to 6, carbon atoms. “Lower monovalent hydrocarbon group” means a monovalent hydrocarbon group having 1 to 4, preferably 1 to 3 carbon atoms, more preferably 1 to 2, carbon atoms. Preferred lower monovalent hydrocarbon groups include alkyl groups such as methyl and ethyl. Monovalent hydrocarbon groups may have a straight chain or branched chain structure. Preferred monovalent hydrocarbon groups have one or two branches, preferably 1 branch. Preferred monovalent hydrocarbon groups are saturated. Unsaturated monovalent hydrocarbon groups have one or more double bonds, one or more triple bonds, or combinations thereof. Preferred unsaturated monovalent hydrocarbon groups have one or two double bonds or one triple bond; more preferred unsaturated monovalent hydrocarbon groups have one double bond.
“Pharmaceutically acceptable” means suitable for use in a human or other mammal.
“Prostaglandin” means a fatty acid derivative which has a variety of potent biological activities of a hormonal or regulatory nature.
“Protecting group” is a group that replaces the active hydrogen of a hydroxyl moiety thus preventing undesired side reaction at the hydroxyl moiety. Use of protecting groups in organic synthesis is well known in the art. Examples of protecting groups are found in Protecting Groups in Organic Synthesis by Greene, T. W. and Wuts, P. G. M., 2nd ed., Wiley & Sons, Inc., 1991. Preferred protecting groups include silyl ethers, alkoxymethyl ethers, tetrahydropyranyl, tetrahydrofuranyl, esters, and substituted or unsubstituted benzyl ethers.
“Selective” means having a binding or activation preference for a specific receptor over other receptors which can be quantitated based upon receptor binding or activation assays.
“Subject” means a living vertebrate animal such as a mammal (preferably human) in need of treatment.
“Substituted aromatic group” means an aromatic group wherein 1 to 4 of the hydrogen atoms bonded to carbon atoms in the ring have been replaced with other substituents. Preferred substituents include: halogen atoms, cyano groups, monovalent hydrocarbon groups, substituted monovalent hydrocarbon groups, heterogeneous groups, aromatic groups, substituted aromatic groups, or any combination thereof. More preferred substituents include halogen atoms, monovalent hydrocarbon groups, and substituted monovalent hydrocarbon groups. Preferred substituted aromatic groups include naphthyl. The substituents may be substituted at the ortho, meta, or para position on the ring, or any combination thereof. The preferred substitution pattern on the ring is ortho or meta. The most preferred substitution pattern is ortho.
“Substituted carbocyclic group” means a carbocyclic group wherein 1 to 4 hydrogen atoms bonded to carbon atoms in the ring have been replaced with other substituents. Preferred substituents include: halogen atoms, cyano groups, monovalent hydrocarbon groups, monovalent heterogeneous groups, substituted monovalent hydrocarbon groups, aromatic groups, substituted aromatic groups, or any combination thereof. More preferred substituents include halogen atoms and substituted monovalent hydrocarbon groups. Carbocyclic group does not include aromatic rings.
“Substituted heteroaromatic group” means a heteroaromatic group wherein 1 to 4 hydrogen atoms bonded to carbon atoms in the ring have been replaced with other substituents. Preferred substituents include: halogen atoms, cyano groups, monovalent hydrocarbon groups, substituted monovalent hydrocarbon groups, heterogeneous groups, substituted heterogeneous groups, phenyl groups, phenoxy groups, or any combination thereof. More preferred substituents include halogen atoms, halogenated hydrocarbon groups, monovalent hydrocarbon groups, and phenyl groups.
“Substituted heterocyclic group” means a heterocyclic group wherein 1 to 4 hydrogen atoms bonded to carbon atoms in the ring have been replaced with other substituents. Preferred substituents include: halogen atoms, cyano groups, monovalent hydrocarbon groups, substituted monovalent hydrocarbon groups, heterogeneous groups, substituted heterogeneous groups, halogenated hydrocarbon groups, phenyl groups, phenoxy groups, or any combination thereof. More preferred substituents include halogen atoms and halogenated hydrocarbon groups. Substituted heterocyclic groups are not aromatic.
“Substituted heterogeneous group” means a heterogeneous group, wherein 1 to 4 of the hydrogen atoms bonded to carbon atoms in the chain have been replaced with other substituents. Preferred substituents include halogen atoms, hydroxy groups, alkoxy groups (e.g., methoxy, ethoxy, propoxy, butoxy, and pentoxy), aryloxy groups (e.g., phenoxy, chlorophenoxy, tolyloxy, methoxyphenoxy, benzyloxy, alkyloxycarbonylphenoxy, and acyloxyphenoxy), acyloxy groups (e.g., propionyloxy, benzoyloxy, and acetoxy), carbamoyloxy groups, carboxy groups, mercapto groups, alkylthio groups, acylthio groups, arylthio groups (e.g., phenylthio, chlorophenylthio, alkylphenylthio, alkoxyphenylthio, benzylthio, and alkyloxycarbonylphenylthio), aromatic groups (e.g., phenyl and tolyl), substituted aromatic groups (e.g., alkoxphenyl, alkoxycarbonylphenyl, and halophenyl), heterocyclic groups, heteroaromatic groups, and amino groups (e.g., amino, mono- and di-alkylamino having 1 to 3 carbon atoms, methylphenylamino, methylbenzylamino, alkanylamido groups of 1 to 3 carbon atoms, carbamamido, ureido, and guanidino).
“Substituted monovalent hydrocarbon group” means a monovalent hydrocarbon group wherein 1 to 4 of the hydrogen atoms bonded to carbon atoms in the chain have been replaced with other substituents. Preferred substituents include halogen atoms; halogenated hydrocarbon groups; alkyl groups (e.g., methyl, ethyl, propyl, and butyl); hydroxy groups; alkoxy groups (e.g., methoxy, ethoxy, propoxy, butoxy, and pentoxy); aryloxy groups (e.g., phenoxy, chlorophenoxy, tolyloxy, methoxyphenoxy, benzyloxy, alkyloxycarbonylphenoxy, and acyloxyphenoxy); acyloxy groups (e.g., propionyloxy, benzoyloxy, and acetoxy); carbamoyloxy groups; carboxy groups; mercapto groups; alkylthio groups; acylthio groups; arylthio groups (e.g., phenylthio, chlorophenylthio, alkylphenylthio, alkoxyphenylthio, benzylthio, and alkyloxycarbonylphenylthio); aryl groups (e.g., phenyl, tolyl, alkoxyphenyl, alkoxycarbonylphenyl, and halophenyl); heterocyclyic groups; heteroaryl groups; and amino groups (e.g., amino, mono- and di-alkanylamino groups of 1 to 3 carbon atoms, methylphenylamino, methylbenzylamino, alkanylamido groups of 1 to 3 carbon atoms, carbamamido, ureido, and guanidino).
“TP receptor” means known human TP receptors, their splice variants, and undescribed receptors that have similar binding and activation profiles as the known human TP receptors.
2-Decarboxy-2-phosphinico prostaglandin derivatives
The 2-decarboxy-2-phosphinico prostaglandin derivatives comprise a modified α-chain having a phosphinico group at position 2 and an Ω-chain, both bonded to a 5-membered ring structure. The modified α-chain has the formula




Bond k is selected from the group consisting of a single bond and a double bond. Bond k is preferably a single bond.
R1 is selected from the group consisting of a hydrogen atom, a monovalent hydrocarbon group having 1 to 4 carbon atoms, and a monovalent heterogenous group having 1 to 4 member atoms. Preferably R1 is a hydrogen atom or a monovalent hydrocarbon group. When R1 is a monovalent hydrocarbon group, it preferably has 1 to 3 carbon atoms, more preferably 1 to 2 carbon atoms, most preferably 1 carbon atom. Most preferably, R1 is a methyl group.
R2 is selected from the group consisting of a hydrogen atom, a monovalent hydrocarbon group, a substituted monovalent hydrocarbon group, a monovalent heterogeneous group, a substituted monovalent heterogeneous group, a carbocyclic group, a substituted carbocyclic group, a heterocyclic group, a substituted heterocyclic group, an aromatic group, a substituted aromatic group, a heteroaromatic group, and a substituted heteroaromatic group. Preferably, R2 is a hydrogen atom.
R3 is selected from the group consisting of an oxygen atom, a sulfur atom, and NH. Preferably, R3 is an oxygen atom.
R4 is selected from the group consisting of an oxygen atom and a sulfur atom. R4 is preferably an oxygen atom.
R5 is a divalent group. R5 is selected from the group consisting of a hydrocarbon group, a substituted hydrocarbon group, a heterogeneous group, and a substituted heterogeneous group. When R5 is a heterogeneous group, R5 has only one heteroatom, which is selected from the group consisting of oxygen, sulfur, and nitrogen. R5 preferably has 1 to 5 member atoms when R5 is a heterogenous group. R5 preferably has 1 to 5 carbon atoms in the chain when R5 is a hydrocarbon group. R5 is preferably a hydrocarbon group. R5 may be saturated or unsaturated. R5 preferably has a cis double bond at the C5–C6 position in the modified α-chain.
R18 is selected from the group consisting of a sulfur atom and —CH2—. R18 is preferably —CH2—.
In a preferred embodiment of the invention, the modified α-chain has 5 to 8 member atoms from the first carbon atom after the ring up to and including the phosphorous atom.
The Ω-chain has the formula




wherein bond a is selected from the group consisting of a single bond, a trans double bond, and a triple bond. Preferably, bond a is a single bond or a cis double bond.

R6 is a bond or a divalent group selected from the group consisting of —CH2—, —C(O)— and —C(R10)(OR10)—. Preferably, R6 is —C(R10)(OR10)—.
R7 is a bond or a divalent group having the formula —(CD(D))p-X—(CD(D))q-, wherein p is an integer from 0 to 3 and q is an integer from 0 to 3, X is selected from the group consisting of an oxygen atom, a divalent hydrocarbon group, a sulfur atom, SO, SO2, and ND, and each D is independently selected from the group consisting of a hydrogen atom, a monovalent hydrocarbon group of 1 to 4 carbon atoms, and a monovalent heterogenous group of 1 to 4 member atoms. R7 is preferably selected from the group consisting of —CH2O—, —CH═CH—, —CH═C═CH—, —CH2S—, —CH2CH2—, —CH2NH—, —CH2NCH2—, and —CH2O(CH2)3O—.
R8 is selected from the group consisting of a hydrocarbon group, a substituted hydrocarbon group, a heterogenous group, a substituted heterogenous group, a carbocyclic group, a substituted carbocyclic group, a heterocyclic group, a substituted heterocyclic group, an aromatic group, a substituted aromatic group, a heteroaromatic group, and a substituted heteroaromatic group. When R8 is a monocyclic group, it has 5 to 10 member atoms. When R8 is a bicyclic group, it has 8 to 12 member atoms. R8 is preferably selected from the group consisting of a methyl group, aromatic groups, substituted aromatic groups, heteroaromatic groups, and substituted heteroaromatic groups.
R10 is selected from the group consisting of a hydrogen atom, a monovalent hydrocarbon group of 1 to 4 carbon atoms, and a monovalent heterogenous group of 1 to 4 member atoms. Preferably, R10 is a hydrogen atom or a monovalent hydrocarbon group of 1 to 4 carbon atoms. When R10 is a monovalent hydrocarbon group, R10 preferably has 1 carbon atom. Most preferably, R10 is a hydrogen atom.
R16 is selected from the group consisting of —CH2—, —NH—, and —NR19—, wherein R19 is selected from the group consisting of hydrocarbon groups, substituted hydrocarbon groups, heterogenous groups, and substituted heterogenous groups; with the proviso that R19 may optionally be bonded together with R8 to form a ring structure selected from the group consisting of heterocyclic groups and substituted heterocyclic groups. R16 is preferably —CH2—.
R17 is selected from the group consisting of —SO2—, C(O)—, and —CH2—. R17 is preferably —CH2—.
In a preferred embodiment of the invention, the Ω-chain has 7 to 12 member atoms in the chain from the first carbon atom after the ring to R8.
The ring structure is a 5-membered selected from the group consisting of a carbocyclic group, a substituted carbocyclic group, a heterocyclic group, and a substituted heterocyclic group.
The prostaglandin derivatives of this invention preferably have the general formula:




wherein bond a, bond k, R1, R2, R3, R4, R5, R6, R7, R8, R16, R17, and R18 are as described above.

Each of bonds b, c, d, e, and f are independently selected from the group consisting of a single bond and a double bond. Preferably, 0 to 1 of bonds b, c, d, e, and f is a double bond. More preferably, each of bonds b, c, d, e, and f is a single bond.
Each of bonds g, h, i, and j are independently selected from the group consisting of nil, a single bond, and a double bond. Preferably, bond h is a single bond.
M1, M2, M3, and M4 are each independently selected from the group consisting of a carbon atom and a heteroatom. No two heteroatoms are adjacent. No more than two of M1, M2, M3, and M4 are heteroatoms. Preferably, no more than one of M1, M2, M3, and M4 is a heteroatom. Preferred heteroatoms for M1, M2, M3, and M4 include nitrogen and oxygen. More preferably, M1, M2, M3, and M4 are each carbon atoms.
R11, R12, R13, and R15 are each independently selected from the group consisting of nil, a halogen atom, a heteroatom, and R2, with the proviso that, optionally, R11 and R12, R12 and R13, or R11 and R13 may be bonded together to form a ring structure such as a carbocyclic group, a heterocyclic group, an aromatic group, a heteroaromatic group, a substituted carbocyclic group, a substituted heterocyclic group, a substituted aromatic group, or a substituted heteroaromatic group. R12 is preferably a hydrogen atom. R11 is preferably selected from the group consisting of a hydrogen atom; an oxygen atom (i.e., when bond i is a double bond); and OR9, wherein R9 is selected from the group consisting of a hydrogen atom, a monovalent hydrocarbon group of 1 to 4 carbon atoms, and a monovalent heterogenous group of 1 to 4 member atoms.
When R11 is OR9, R12 is a hydrogen atom, and M2 is a carbon atom; R13 is not a hydrogen atom, OR9, a monovalent hydrocarbon group of 1 to 4 carbon atoms, a monovalent heterogenous group of 1 to 4 carbon atoms, a substituted monovalent hydrocarbon group of 1 to 4 carbon atoms, or a substituted monovalent heterogenous group of 1 to 4 carbon atoms.
R13 is not N(R9)(OR9) when bond g is a single bond and R13 is not NOR9 when bond g is a double bond. R13 is preferably selected from the group consisting of a hydrogen atom; an oxygen atom (i.e., when bond i is a double bond); and OR9; with the proviso that R13 is not OR9 when R11 is OR9, M1, M2, M3, and M4 are each carbon atoms, and R12 is a hydrogen atom.
Preferred prostaglandin derivatives of the above formula are selected from the group consisting of PGD, PGE, PGI, and thromboxane derivatives.
Derivatives suitable to use in this invention may also be any optical isomer, diastereomer, and enantiomer of the above structure; or any pharmaceutically-acceptable salts of the above structure; or any biohydrolyzable amides, esters, and imides of the above structure; or combinations thereof.
Typically, the 2-decarboxy-2-phosphinico prostaglandin derivative of the general formula described above has a formula selected from the group consisting of:










wherein bond a, bond k, R1, R2, R3, R4, R5, R6, R7, R8, R9, R16, and R17 are as described above, and each R14 is independently selected from the group consisting of nil, a hydrogen atom, a halogen atom, a monovalent hydrocarbon group of 1 to 4 carbon atoms, and a monovalent heterogenous group of 1 to 4 member atoms. Preferably, R9 is a hydrogen atom.

Compositions
This invention further relates to compositions containing a prostaglandin derivative described above as an active ingredient (hereinafter, component A). The compositions can be pharmaceutical or cosmetic compositions, administered for treatment or prophylaxis of various conditions. Standard pharmaceutical formulation techniques are used, such as those disclosed in Remington's Pharmaceutical Sciences, Mack Publishing Company, Easton, Pa. (1990).
The composition further comprises component B) a carrier. “Carrier” means one or more compatible substances that are suitable for administration to a mammal. Carrier includes solid or liquid diluents, hydrotropes, surface-active agents, and encapsulating substances. “Compatible” means that the components of the composition are capable of being commingled with component A), and with each other, in a manner such that there is no interaction which would substantially reduce the efficacy of the composition under ordinary use situations. Carriers must be of sufficiently high purity and sufficiently low toxicity to render them suitable for administration to the mammal being treated. The carrier can be inert, or it can possess pharmaceutical benefits, cosmetic benefits, or both.
The choice of carrier for component B) depends on the route by which component A) will be administered and the form of the composition. The composition may be in a variety of forms, suitable, for example, for systemic administration (e.g., oral, rectal, nasal, sublingual, buccal, or parenteral) or topical administration (e.g., local application on the skin, ocular, liposome delivery systems, or iontophoresis).
Carriers for systemic administration typically comprise one or more ingredients selected from the group consisting of a) diluents, b) lubricants, c) binders, d) disintegrants, e) colorants, f) flavors, g) sweeteners, h) antioxidants, j) preservatives, k) glidants, m) solvents, n) suspending agents, o) wetting agents, p) surfactants, combinations thereof, and others.
Ingredient a) is a diluent. Suitable diluents include sugars such as glucose, lactose, dextrose, and sucrose; polyols such as propylene glycol; calcium carbonate; sodium carbonate; glycerin; mannitol; and sorbitol. The amount of ingredient a) in the systemic composition is typically about 50 to about 90%.
Ingredient b) is a lubricant. Suitable lubricants are exemplified by solid lubricants including silica, talc, stearic acid and its magnesium salts and calcium salts, calcium sulfate; and liquid lubricants such as polyethylene glycol and vegetable oils such as peanut oil, cottonseed oil, sesame oil, olive oil, corn oil and oil of theobroma. The amount of ingredient b) in the systemic composition is typically about 5 to about 10%.
Ingredient c) is a binder. Suitable binders include polyvinylpyrrolidone; magnesium aluminum silicate; starches such as corn starch and potato starch; gelatin; tragacanth; and cellulose and its derivatives, such as sodium carboxymethylcellulose, ethyl cellulose, methylcellulose, microcrystalline cellulose, and sodium carboxymethylcellulose. The amount of ingredient c) in the systemic composition is typically about 5 to about 50%.
Ingredient d) is a disintegrant. Suitable disintegrants include agar, alginic acid and the sodium salt thereof, effervescent mixtures, croscarmelose, crospovidone, sodium carboxymethyl starch, sodium starch glycolate, clays, and ion exchange resins. The amount of ingredient d) in the systemic composition is typically about 0.1 to about 10%.
Ingredient e) is a colorant such as an FD&C dye. The amount of ingredient e) in the systemic composition is typically about 0.005 to about 0.1%.
Ingredient f) is a flavor such as menthol, peppermint, and fruit flavors. The amount of ingredient f) in the systemic composition is typically about 0.1 to about 1.0%.
Ingredient g) is a sweetener such as aspartame and saccharin. The amount of ingredient g) in the systemic composition is typically about 0.001 to about 1%.
Ingredient h) is an antioxidant such as butylated hydroxyanisole (“BHA”), butylated hydroxytoluene (“BHT”), and vitamin E. The amount of ingredient h) in the systemic composition is typically about 1 to about 5%.
Ingredient j) is a preservative such as methyl paraben and sodium benzoate. The amount of ingredient j) in the systemic composition is typically about 1 to about 5%.
Ingredient k) is a glidant such as silicon dioxide. The amount of ingredient k) in the systemic composition is typically about 1 to about 5%.
Ingredient m) is a solvent, such as water, isotonic saline, ethyl oleate, alcohols such as ethanol, and phosphate buffer solutions. The amount of ingredient m) in the systemic composition is typically 0 to about 10%.
Ingredient n) is a suspending agent. Suitable suspending agents include AVICEL® RC-591 from FMC Corporation of Philadelphia, Pa. and sodium alginate. The amount of ingredient n) in the systemic composition is typically about 1 to about 8%.
Ingredient o) is a surfactant such as lecithin, polysorbate 80, and sodium lauryl sulfate, and the TWEENS® from Atlas Powder Company of Wilmington, Del. Suitable surfactants include those disclosed in the C.T.F.A. Cosmetic Ingredient Handbook, 1992, pp.587–592; Remington's Pharmaceutical Sciences, 15th Ed. 1975, pp. 335–337; and McCutcheon's Volume 1, Emulsifiers & Detergents, 1994, North American Edition, pp. 236–239. The amount of ingredient o) in the systemic composition is typically about 0.1 to about 2%.
Although the amounts of components A) and B) in the systemic compositions will vary depending on the type of systemic composition prepared, the specific derivative selected for component A) and the ingredients of component B), in general, system compositions comprise 0.01% to 50% of component A) and 50 to 99.99% of component B).
Compositions for parenteral administration typically comprise A) 0.1 to 10% of a prostaglandin and B) 90 to 99.9% of a carrier comprising a) a diluent and m) a solvent. Preferably, component a) is propylene glycol and m) is ethanol or ethyl oleate.
Compositions for oral administration can have various dosage forms. For example, solid forms include tablets, capsules, granules, and bulk powders. These oral dosage forms comprise a safe and effective amount, usually at least 5%, and preferably from 25% to 50%, of component A). The oral dosage compositions further comprise 50 to 95% of component B), preferably 50 to 75%.
Tablets can be compressed, tablet triturates, enteric-coated, sugar-coated, film-coated. coated, or multiple-compressed. Tablets typically comprise component A), and component B) a carrier comprising ingredients selected from the group consisting of a) diluents, b) lubricants, c) binders, d) disintegrants, e) colorants, f) flavors, g) sweeteners, k) glidants, and combinations thereof. Preferred diluents include calcium carbonate, sodium carbonate, mannitol, lactose and cellulose. Preferred binders include starch, gelatin, and sucrose. Preferred disintegrants include alginic acid and croscarmelose. Preferred lubricants include magnesium stearate, stearic acid, and talc. Preferred colorants are the FD&C dyes, which can be added for appearance. Chewable tablets preferably contain g) sweeteners such as aspartame and saccharin, or f) flavors such as menthol, peppermint, and fruit flavors, or both.
Capsules (including time release and sustained release formulations) typically comprise component A), and B) a carrier comprising one or more a) diluents disclosed above in a capsule comprising gelatin. Granules typically comprise component A), and preferably further comprise k) glidants such as silicon dioxide to improve flow characteristics.
The selection of ingredients in the carrier for oral compositions depends on secondary considerations like taste, cost, and shelf stability, which are not critical for the purposes of this invention. One skilled in the art would know how to select appropriate ingredients without undue experimentation.
The solid compositions may also be coated by conventional methods, typically with pH or time-dependent coatings, such that A) the prostaglandin is released in the gastrointestinal tract in the vicinity of the desired application, or at various times to extend the desired action. The coatings typically comprise one or more components selected from the group consisting of cellulose acetate phthalate, polyvinylacetate phthalate, hydroxypropyl methyl cellulose phthalate, ethyl cellulose, EUDRAGIT® coatings (available from Rohm & Haas G.M.B.H. of Darmstadt, Germany), waxes and shellac.
Compositions for oral administration can also have liquid forms. For example, suitable liquid forms include aqueous solutions, emulsions, suspensions, solutions reconstituted from non-effervescent granules, suspensions reconstituted from non-effervescent granules, effervescent preparations reconstituted from effervescent granules, elixirs, tinctures, syrups, and the like. Liquid orally administered compositions typically comprise A) the prostaglandin and B) a carrier comprising ingredients selected from the group consisting of a) diluents, e) colorants, f) flavors, g) sweeteners, j) preservatives, m) solvents, n) suspending agents, and o) surfactants. Peroral liquid compositions preferably comprise one or more ingredients selected from the group consisting of e) colorants, f) flavors, and g) sweeteners.
Other compositions useful for attaining systemic delivery of the subject compounds include sublingual, buccal and nasal dosage forms. Such compositions typically comprise one or more of soluble filler substances such as a) diluents including sucrose, sorbitol and mannitol; and c) binders such as acacia, microcrystalline cellulose, carboxymethylcellulose, and hydroxypropylmethylcellulose. Such compositions may further comprise b) lubricants, e) colorants, f) flavors, g) sweeteners, h) antioxidants, and k) glidants.
In an alternative embodiment of the invention, the prostaglandins are topically administered. Topical compositions that can be applied locally to the skin may be in any form including solutions, oils, creams, ointments, gels, lotions, shampoos, leave-on and rinse-out hair conditioners, milks, cleansers, moisturizers, sprays, skin patches, and the like. Topical compositions comprise: component A) the prostaglandin described above and component B) a carrier. The carrier of the topical composition preferably aids penetration of the prostaglandins into the skin. Component B) may further comprise one or more optional components.
The exact amounts of each component in the topical composition depend on various factors. The amount of component A) added to the topical composition is:

IC50×10−2≧% of component A)≧IC50×10−3,

where IC50 of component A) is expressed in nanomolar units. For example, if the IC50 of the prostaglandin is 1 nM, the amount of component A) will be 0.001 to 0.01%. If the IC50 of the prostaglandin is 10 nM, the amount of component A) will be 0.01 to 0.1%. If the IC50 of the prostaglandin is 100 nM, the amount of component A) will be 0.1 to 1.0%. If the IC50 of the prostaglandin is 1000 nM, the amount of component A) will be 1.0 to 10%, preferably 1.0 to 5%. The amount and dosage of component A) are critical. If the amount of component A) is outside the ranges specified above (i.e., either higher or lower), efficacy of the treatment may be reduced. IC50 can be calculated according to the method in Reference Example 1, below. One skilled in the art would know how to calculated IC50. The remainder of the composition, up to 100%, is component B).

The amount of B) the carrier employed in conjunction with component A) is sufficient to provide a practical quantity of composition for administration per unit dose of the prostaglandin. Techniques and compositions for making dosage forms useful in the methods of this invention are described in the following references: Modern Pharmaceutics, Chapters 9 and 10, Banker & Rhodes, eds. (1979); Lieberman et al., Pharmaceutical Dosage Forms: Tablets (1981); and Ansel, Introduction to Pharmaceutical Dosage Forms, 2nd Ed., (1976).
Component B) the carrier may comprise a single ingredient or a combination of two or more ingredients. In the topical compositions, component B) is a topical carrier. Preferred topical carriers include one or more ingredients selected from the group consisting of water, alcohols, aloe vera gel, allantoin, glycerin, vitamin A and E oils, mineral oil, propylene glycol, PPG-2 myristyl propionate, dimethyl isosorbide, combinations thereof, and the like. More preferred carriers include propylene glycol, dimethyl isosorbide, and water.
The carrier of the topical composition may further comprise one or more ingredients selected from the group consisting of q) emollients, r) propellants, s) solvents, t) humectants, u) thickeners, v) powders, w) fragrances, and x) pigments.
Ingredient q) is an emollient. The amount of ingredient q) in the topical composition is typically 5 to 95%. Suitable emollients include stearyl alcohol, glyceryl monoricinoleate, glyceryl monostearate, propane-1,2-diol, butane-1,3-diol, mink oil, cetyl alcohol, isopropyl isostearate, stearic acid, isobutyl palmitate, isocetyl stearate, oleyl alcohol, isopropyl laurate, hexyl laurate, decyl oleate, octadecan-2-ol, isocetyl alcohol, cetyl palmitate, di-n-butyl sebacate, isopropyl myristate, isopropyl palmitate, isopropyl stearate, butyl stearate, polyethylene glycol, triethylene glycol, lanolin, sesame oil, coconut oil, arachis oil, castor oil, acetylated lanolin alcohols, petroleum, mineral oil, butyl myristate, isostearic acid, palmitic acid, isopropyl linoleate, lauryl lactate, myristyl lactate, decyl oleate, myristyl myristate, and combinations thereof. Preferred emollients include stearyl alcohol and polydimethylsiloxane.
Ingredient r) is a propellant. The amount of ingredient r) in the topical composition is typically 5 to 95%. Suitable propellants include propane, butane, isobutane, dimethyl ether, carbon dioxide, nitrous oxide, and combinations thereof.
Ingredient s) is a solvent. The amount of ingredient s) in the topical composition is typically 5 to 95%. Suitable solvents include water, ethyl alcohol, methylene chloride, isopropanol, castor oil, ethylene glycol monoethyl ether, diethylene glycol monobutyl ether, diethylene glycol monoethyl ether, dimethylsulfoxide, dimethyl formamide, tetrahydrofuran, and combinations thereof. Preferred solvents include ethyl alcohol.
Ingredient t) is a humectant. The amount of ingredient t) in the topical composition is typically 5 to 95%. Suitable humectants include glycerin, sorbitol, sodium 2-pyrrolidone-5-carboxylate, soluble collagen, dibutyl phthalate, gelatin, and combinations thereof. Preferred humectants include glycerin.
Ingredient u) is a thickener. The amount of ingredient u) in the topical composition is typically 0 to 95%.
Ingredient v) is a powder. The amount of ingredient v) in the topical composition is typically 0 to 95%. Suitable powders include chalk, talc, fullers earth, kaolin, starch, gums, colloidal silicon dioxide, sodium polyacrylate, tetra alkyl ammonium smectites, trialkyl aryl ammonium smectites, chemically modified magnesium aluminum silicate, organically modified montmorillonite clay, hydrated aluminum silicate, fumed silica, carboxyvinyl polymer, sodium carboxymethyl cellulose, ethylene glycol monostearate, and combinations thereof.
Ingredient w) is a fragrance. The amount of ingredient w) in the topical composition is typically 0.001 to 0.5%, preferably 0.001 to 0.1%.
Ingredient x) is a pigment. Suitable pigments include inorganic pigments, organic lake pigments, pearlescent pigments, and mixtures thereof. Inorganic pigments useful in this invention include those selected from the group consisting of rutile or anatase titanium dioxide, coded in the Color Index under the reference CI 77,891; black, yellow, red and brown iron oxides, coded under references CI 77,499, 77,492 and, 77,491; manganese violet (CI 77,742); ultramarine blue (CI 77,007); chromium oxide (CI 77,288); chromium hydrate (CI 77,289); and ferric blue (CI 77,510) and mixtures thereof.
The organic pigments and lakes useful in this invention include those selected from the group consisting of D&C Red No. 19 (CI 45,170), D&C Red No. 9 (CI 15,585), D&C Red No. 21 (CI 45,380), D&C Orange No. 4 (CI 15,510), D&C Orange No. 5 (CI 45,370), D&C Red No. 27 (CI 45,410), D&C Red No. 13 (CI 15,630), D&C Red No. 7 (CI 15,850), D&C Red No. 6 (CI 15,850), D&C Yellow No. 5 (CI 19,140), D&C Red No. 36 (CI 12,085), D&C Orange No. 10 (CI 45,425), D&C Yellow No. 6 (CI 15,985), D&C Red No. 30 (CI 73,360), D&C Red No. 3 (CI 45,430) and the dye or lakes based on Cochineal Carmine (CI 75,570) and mixtures thereof.
The pearlescent pigments useful in this invention include those selected from the group consisting of the white pearlescent pigments such as mica coated with titanium oxide, bismuth oxychloride, colored pearlescent pigments such as titanium mica with iron oxides, titanium mica with ferric blue, chromium oxide and the like, titanium mica with an organic pigment of the above-mentioned type as well as those based on bismuth oxychloride and mixtures thereof. The amount of pigment in the topical composition is typically 0 to 10%.
In an alternative embodiment of the invention, topical pharmaceutical compositions for ocular administration are prepared by conventional methods. Topical pharmaceutical compositions for ocular administration typically comprise component A) and B) a carrier, such as purified water, and one or more ingredients selected from the group consisting of y) sugars such as dextrans, particularly dextran 70, z) cellulose or a derivative thereof, aa) a salt, bb) disodium EDTA (Edetate disodium), and cc) a pH adjusting additive.
Examples of z) cellulose derivatives suitable for use in the topical pharmaceutical composition for ocular administration include sodium carboxymethylcellulose, ethylcellulose, methylcellulose, and hydroxypropylmethylcellulose. Hydroxypropylmethylcellulose is preferred.
Examples of aa) salts suitable for use in the for use in the topical pharmaceutical composition for ocular administration include sodium chloride, potassium chloride, and combinations thereof.
Examples of cc) pH adjusting additives include HCl or NaOH in amounts sufficient to adjust the pH of the topical pharmaceutical composition for ocular administration to 7.2–7.5.
Component A) may also be administered in the form of liposome delivery systems, such as small unilamellar vesicles, large unilamellar vesicles, and multilamellar vesicles. Liposomes can be formed from a variety of phospholipids, such as cholesterol, stearylamine or phosphatidylcholines. A preferred formulation for topical delivery of the present compounds uses liposomes as described in Dowton et al., Influence of Liposomal Composition on Topical Delivery of Encapsulated Cyclosporin A: I. An in vitro Study Using Hairless Mouse Skin, S.T.P. Pharma Sciences, 1993 Vol. 3, pp. 404–407; Wallach and Philippot, New Type of Lipid Vesicle: Novasome®, Liposome Technology, 1993 Vol. 1, pp. 141–156; U.S. Pat. Nos. 4,911,928; 5,834,014.
Component A) may also be administered by iontophoresis (see, e.g., Internet site www.unipr.it/arpa/dipfarm/erasmus/erasm14.html; Banga et al., Hydrogel-based Iontotherapeutic Delivery Devices for Transdermal Delivery of Peptide/Protein Drugs, Pharm. Res. 1993 Vol. 10(5), pp. 697–702; Ferry, Theoretical Model of Iontophoresis Utilized in Transdermal Drug Delivery, Pharmaceutical Acta Helvetiae, 1995 Vol 70, pp. 279–287; Gangarosa et al., Modern Iontophoresis for Local Drug Delivery, Int. J. Pharm, 1995 Vol. 123, pp. 159–171; Green et al., Iontophoretic Delivery of a Series of Tripeptides Across the Skin in vitro, Pharm. Res., 1991 Vol 8, pp. 1121–1127; Jadoul et al., Quantification and Localization of Fentanyl and TRH Delivered by Iontophoresis in the Skin, Int. J. Pharm. 1995 Vol. 120, pp. 221–8; O'Brien et al., An Updated Review of its Antiviral Activity, Pharmacokinetic Properties and Therapeutic Efficacy, Drugs, 1989Vol. 37, pp. 233–309; Parry et al., Acyclovir Biovailability in Human Skin, J. Invest. Dermatol., 1992 Vol. 98 (6), pp. 856–63; Santi et al., Drug Reservoir Composition and Transport of Salmon Calcitonin in Transdermal Iontophoresis, Pharm. Res., 1997 Vol 14 (1), pp. 63–66; Santi et al., Reverse Iontophoresis—Parameters Determining Electroosmotic Flow: I. pH and Ionic Strength, J. Control. Release, 1996 Vol. 38, pp. 159–165; Santi et al., Reverse Iontophoresis—Parameters Determining Electroosmotic Flow: II. Electrode Chamber Formulation, J. Control. Release, 1996 Vol. 42, pp. 29–36; Rao et al., Reverse Iontophoresis: Noninvasive Glucose Monitoring in vivo in Humans, Pharm. Res., 1995 Vol. 12 (12), pp. 1869–1873; Thysman et al., Human Calcitonin Delivery in Rats by Iontophoresis, J. Pharm. Pharmacol., 1994 Vol. 46, pp. 725–730; and Volpato et al., Iontophoresis Enhances the Transport of Acyclovir through Nude Mouse Skin by Electrorepulsion and Electroosmosis, Pharm. Res., 1995 Vol. 12 (11), pp. 1623–1627.
Component A) may be included in kits comprising component A), a systemic or topical composition described above, or both; and information, instructions, or both that use of the kit will provide treatment for cosmetic and medical conditions in mammals (particularly humans). The information and instructions may be in the form of words, pictures, or both, and the like. In addition or in the alternative, the kit may comprise a prostaglandin derivative, a composition, or both; and information, instructions, or both, regarding methods of application of the prostaglandin derivative or composition, preferably with the benefit of treating or preventing cosmetic and medical conditions in mammals.
Use of the Prostaglandin Derivatives and Compositions
This invention further relates to methods for treating medical and cosmetic conditions in mammals. The prostaglandin derivatives, and compositions containing the derivatives, of this invention can be used to treat subjects suffering from many medical and cosmetic conditions. Generally, the method comprises administering to a mammal in need of treatment, a prostaglandin derivative (or composition) described above.
The conditions that can be treated depend on the type of derivative, i.e., the receptor or receptors for which the derivative has affinity and whether the derivative is an agonist or antagonist for the receptor.
Whether the derivative has affinity for the receptor typically depends on the substituent groups bonded to the ring (R11 or R13, or both, in the general structure above). For example, in PGE derivatives, which have affinity for the EP1, EP2, EP3, and EP4, receptors, typically R11 is an oxygen atom and bond i is a double bond.
Whether the derivative is an agonist or antagonist of the receptor typically depends on the stereochemistry of the ring and the Ω-chain up to and including R6 in the general formula above. If the stereochemistry of this portion of the derivative is the same as, or similar to, that of the naturally occurring prostaglandin, the derivative will be an agonist. If the stereochemistry differs from that of the naturally occurring prostaglandin, the derivative can be an antagonist. For example, naturally occurring PGE2 has the formula:




A PGE derivative according to this invention has the formula:





The α-configuration of the bond at position C15 is the same in both naturally occurring PGE2 and the PGE derivative of this invention, indicating that the PGE derivative is an agonist.

In contrast, naturally occurring PGF2α has the formula:




A PGF derivative according to this invention has the formula:




Naturally occurring PGF2α has a bond in the alpha configuration at position C11. The PGF derivative has a bond in the beta configuration at position C11. The difference in bond configuration at position C11 indicates that the PGF derivative may be an antagonist.
Selectivity of prostaglandin derivatives toward different receptors can be controlled by varying certain groups at specified positions in the prostaglandin derivative. (Numbering is based on that of naturally-occurring prostaglandins, so they must be translated to the appropriate position on the derivative.) A 9-ketone or other sp2-hybridized group is preferred for the derivative to have selectivity toward the EP receptors. A 9-hydroxyl and a 15-hydroxyl are preferred for the derivative to have selectivity toward the FP receptor. Different groups may be present at position 11, but a hydroxyl group is preferred for PGF derivatives. An 11-ketone or other sp2-hybridized group is preferred for the derivative to have selectivity toward the DP receptor. A bicyclic ring system gives compounds with selectivity toward the IP receptor. A hydrophobic cyclopentyl ring is preferred to confer selectivity to the TP receptor, and a 5,6 alkene is also preferred.
One skilled in the art would, without undue experimentation, be able to determine the receptor for which a derivative has affinity and whether the derivative is an agonist or antagonist, using the above guidelines for determining affinity, and the appropriate biological assays. One skilled in the art would be able to control the receptor for which a derivative has affinity and whether the derivative is an agonist or antagonist using the above guidelines and conventional organic syntheses.
PGE
EP1 agonists can be used to treat bone disorders such as osteoporosis, vascular diseases such as high blood pressure and poor vascular circulation, sexual dysfunction such as erectile dysfunction and women's sexual arousal dysfunction. PGE1 agonists can also be used to enhance skin pigmentation.
EP1 antagonists can be used to treat pain.
EP2 agonists can be used to treat asthma and bone disorders such as osteoporosis. EP2 agonists can be used to inhibit cell migration and protect against neuronal damage in the eye. EP2 agonists can also be used to enhance skin pigmentation.
EP2 antagonists have a diuretic effect and may be used to treat hypertension and premenstrual tension.
EP3 agonists can be used to treat arthritis, bone disorders such as osteoporosis, vascular disease such as high blood pressure and poor vascular circulation. EP3 agonists can also be used to enhance uterine contractions and inhibit gastric acid secretion. EP3 agonists can also be used to prevent or treat, or both, hepatic diseases, renal diseases, pancreatitis, mycardial infarct, and gastric disturbances such as ulcers.
EP3 antagonists can be used to control blood pressure.
EP4 agonists can be used to treat arthritis, bone disorders such as osteoporosis, vascular disease such as high blood pressure and poor vascular circulation, and asthma. EP4 agonists can also be used to inhibit cell migration and prevent neuronal cell death. EP4 agonists can also be used to enhance skin pigmentation.
EP4 antagonists have a diuretic effect and can be used to treat hypertension and premenstrual tension and to lower intraocular pressure to treat conditions such as glaucoma.
PGE derivatives suitable to use to treat the above conditions include those selected from the group consisting of:




wherein bond a, R1, R2, R3, R4, R5, R6, R7, R8, R9, and R14 are as described above. One skilled in the art would be able to select appropriate PGE analogs for each condition without undue experimentation.

PGF
FP agonists can be used to treat bone disorders such as osteoporosis, ocular disorders such as glaucoma, skin disorders, circulatory disorders such as hypertension, gastrointestinal disorders, hair loss, and respiratory disorders. FP agonists can also be used for fertility control, to manage vascular diseases such as diabetic and other forms of peripheral vascular disease, to induce labor, and as nasal decongestants.
FP antagonists can be used to prevent premature labor and to prevent hyper-pigmentation of the skin.
PGF derivatives suitable to treat the above conditions include those having a structure selected from the group consisting of:




wherein bond a, R1, R2, R3, R4, R5, R6, R7, R8, R9, and R14 are as described above.

PGI
IP agonists can be used to treat vascular disorders such as peripheral vascular disease, pulmonary hypertension, high blood pressure and poor vascular circulation; reproductive disorders such as cervical immaturity, to inhibit uterine contractions, and to prevent premature delivery.
Suitable PGI derivatives for treating the above conditions include those having the structure:




wherein bond a, bond k, R1, R2, R3, R4, R5, R6, R7, R8, and R14 are as described above.

PGD
PGD agonists can be used to induce sleep, e.g. for treating sleeping disorders such as insomnia. PGD antagonists can be used to treat allergies. PGD derivatives suitable to treat the above conditons include those having a structure selected from the group consisting of:




wherein bond a, R1, R2, R3, R4, R5, R6, R7, R8, R9, and R14 are as described above.

Thromboxanes
Thromboxane antagonists can be used as anti-allergy agents, to treat vascular disease, and to prevent platelet coagulation.
Suitable thromboxane antagonists for treating the above conditions include those having the structure:




wherein R1, R2, R3, R4, R5, R6, R7, R8, R14, R16, and R17 are as described above.

One skilled in the art will recognize that each of the classifications as a PGE, PGD, PGF, or PGI derivative above is merely exemplary and should not limit the scope of the invention claimed. One skilled in the art would recognize that the structures shown above may have affinity and activity towards more than one receptor. One skilled in the art would be able to determine the receptor for which a structure has affinity without undue experimentation.
The dosage of the prostaglandin derivative administered depends on a variety of factors, including the method of administration. For systemic administration, (e.g., oral, rectal, nasal, sublingual, buccal, or parenteral), typically, 0.5 mg to 300 mg, preferably 0.5 mg to 100 mg, more preferably 0.1 mg to 10 mg, of a prostaglandin derivative described above is administered per day. These dosage ranges are merely exemplary, and daily administration can be adjusted depending on various factors. The specific dosage of the prostaglandin derivative to be administered, as well as the duration of treatment, the condition being treated, and whether the treatment is topical or systemic are interdependent. The dosage and treatment regimen will also depend upon such factors as the condition being treated, the specific prostaglandin derivative used, the treatment indication, the efficacy of the compound, the personal attributes of the subject (such as, for example, weight, age, sex, and medical condition of the subject), compliance with the treatment regimen, and the presence and severity of any side effects of the treatment.
Systemic administration (e.g., parenteral, oral, sublingual, buccal and nasal) is preferably carried out four times per day for the duration of treatment. Systemic administration is preferred for treating allergies and vascular disease such high blood pressure and poor vascular circulation.
For topical administration (e.g., local application on the skin, ocular, liposome delivery systems, or iontophoresis), the topical composition is typically administered once per day for the duration of treatment. For,some conditions, 6 to 12 weeks is sufficient. Topical administration is preferred in treating conditions such as hair loss, treating skin conditions (e.g., enhancing skin pigmentation), treating ocular disorders such as glaucoma, and for local vasodilation.
EXAMPLES
These examples are intended to illustrate the invention to those skilled in the art and should not be interpreted as limiting the scope of the invention set forth in the claims. The compounds of the present invention are prepared according to methods which are known to those skilled in the art. The starting materials used in preparing the compounds of the invention are known, made by known methods, or are commercially available as a starting material.
It is recognized that the skilled artisan in the art of organic chemistry can readily carry out standard manipulations of organic compounds without further direction. Examples of such manipulations are discussed in standard texts such as J. March, Advanced Organic Chemistry, John Wiley & Sons, 1992.
The skilled artisan will readily appreciate that certain reactions are best carried out when other functionalitics are masked or protected in the compound, thus increasing the yield of the reaction and/or avoiding any undesirable side reactions. Often, the skilled artisan utilizes protecting groups to accomplish such increased yields or to avoid the undesired reactions. These reactions are found in the literature and are also well within the scope of the skilled artisan. Examples of many such manipulations can be found in, for example, T. Greene, Protecting Groups in Organic Synthesis, John Wiley & Sons, 1981.
The following abbreviations are used in the examples.
“DMAP” means dimethylaminopyridine.
“Et” means an ethyl group.
“EtOAc” means ethyl acetate.
“LAH” means lithium aluminum hydride.
“Me” means a methyl group.
“MeOH” means methanol.
“PCC” means pyridinium chlorochromate.
“TBDMS” means tert-butyldimethylsilyl.
“TBDMSOTf” means tert-butyldimethylsilyl triflate.
“THF” means tetrahydrofuran.
“TLC” means thin layer chromatography.
“TMS” means trimethylsilyl.
Example 1
Preparation of 2-decarboxy-2-bromo-13,14-dihydryo-17-(3,5-difluorophenyl)-17-trinor-PGF2α11,15-bis tert-butyldimethylsilylate, 9-acetate (E1b)




In a round bottom flask under argon the Wittig salt (a) (2.2 equiv.) is added to THF, and cooled to −78° C. Sodium hexamethyidisilazide (4.4 equiv.) is then added in one portion and the reaction is stirred for 15 minutes at −78° C. The solution is then warmed to 0° C. for two hours. The reaction mixture is recooled to −78° C. and the lactol E1a in THF is added over 10 minutes.
The lactol E1a is prepared from Corey Aldehyde in a manner analogous to that taught in U.S. Pat. No. 6,048,895. The solution is stirred at −78° C. for 1 hour and then allowed to warm to room temperature and stirred an additional 17 hours. The reaction mixture is quenched with water and the THF is removed under reduced pressure. The residue is brought up in EtOAc/hexane and washed two times with 1N HCl. The organic layer is dried with Na2SO4 and solvent is removed under reduced pressure. The residue is taken up in MeOH and TMS diazomethane (5 equiv.) is added slowly to the mixture. The solvent is concentrated and the residue is chromatographed on SiO2 (10% EtOAc/hexanes) to provide the ester as a yellow oil.
To a solution of the ester (1 equiv.) in THF at −78° C. is added Lithium Aluminum Hydride (LAH) (1.3 equiv.) in one portion. The reaction mixture is stirred for 1 hour at −78° C. and then quenched with water. The solution is poured into CH2Cl2 and the aqueous layer is acidified to pH=1. The aqueous layer is re-extracted with CH2Cl2 and organics are combined. The organic layer is dried (Na2SO4) and concentrated. The residue is chromatographed on SiO2 (10% EtOAc/hexanes) to provide the alcohol as a colorless oil.
To a solution of that alcohol and pyridine (1.6 equiv.) in acetonitrile is added, at 0° C., over 10 minutes solid dibromo-triphenylphosphine (1.4 equiv.). The reaction is monitored by TLC (4% EtOAc/hexanes) until complete. The solution is added to 5% EtOAc/hexanes and then the mixture is chromatographed on SiO2 (5% EtOAc/hexanes) to provide the primary bromide as a colorless oil. This product is dissolved in pyridine and acetic anhydride (1.9 equiv.) and DMAP (0.05 equiv.). The reaction is stirred at 25° C. overnight. The reaction is then quenched with water. The organic layer is washed with brine, dried over MgSO4, and concentrated in vacuo to give a crude product which is purified by flash chromatography on silica gel (hexanes then 1% MeOH in CH2Cl2) to 2-decarboxy-2-bromo-13,14-dihydryo-17-(3,5-difluorophenyl)-17-trinor-PGF2α11,15-bis tert-butyldimethylsilylate, 9-acetate (E1b).
Example 2
Preparation of 2-decarboxy-2-(P-methylphosphinico)-13,14-dihydryo-17-(3,5-difluorophenyl)-17-trinor-PGE2 (E2c)




a. 2-decarboxy-2-(O-ethyl-P-methylphosphinico)-13,14-dihydryo-17-(3,5-difluorophenyl)-17-trinor-PGF2α11,15-bis tert-butyldimethylsilylate, 9-acetate (E2a): A mixture of 2-decarboxy-2-bromo-13,14-dihydryo-17-(3,5-difluorophenyl)-17-trinor-PGF2α11,15-bis tert-butyldimethylsilylate, 9-acetate, diethyl methylphosphonite (E1b) and toluene is stirred at 100° C. for 8 hours. The toluene is evaporated and the residue is purified by SiO2 column chromatography (5–25% 2-propanol in 20% dichloromethane-hexane). Appropriate fractions are concentrated, diluted with water, and extracted three times with ethyl acetate. The combined extracts are washed with water, are dried (brine, Na2SO4), and are evaporated to give 2-decarboxy- 2-(O-ethyl-P-methylphosphinico)-13,14-dihydryo-17-(3,5-difluorophenyl)-17-trinor-PGF2α11,15-bis tert-butyldimethylsilylate, 9-acetate (E2a).
b. 2-decarboxy-2-(P-methylphosphinico)-13,14-dihydryo-17-(3,5-difluorophenyl)-17-trinor-PGF1α11,15-bis tert-butyldimethylsilylate (E2b): E2a is added to a 2.5M aqueous sodium hydroxide and is stirred at reflux for 3 hours. The mixture is then diluted with water and is washed two times with ethyl acetate. The combined washes are extracted with water. The combined aqueous phases are acidified with 1M aqueous hydrochloric acid and extracted four times with ethyl acetate. The combined extracts are dried (brine, Na2SO4) then redissolved in methylene chloride and pyridinium chlorochromate is added to effect oxidation of C-9 to the ketone. After three hours, the solution is filtered through silica, and the solvent is evaporated to give 2-decarboxy-2-(P-methylphosphinico)-13,14-dihydryo-17-(3,5-difluorophenyl)-17-trinor-PGE211,15-bis tert-butyldimethylsilylate (E2b).
c. 2-decarboxy-2-(P-methylphosphinico)-13,14-dihydryo-17-(3,5-difluorophenyl)-17-trinor-PGE2 (E2c): The product E2b is dissolved in acetonitrile and HF/pyridine is added at zero degrees centigrade. The reaction is allowed to warm to room temperature, then the crude is concentrated by rotary evaporation, and the residue is chromatographed to yield 2-decarboxy-2-(P-methylphosphinico)-13,14-dihydryo-17-(3,5-difluorophenyl)-17-trinor-PGE2 (E2c):
Examples 3–12
Using largely the procedure set forth in Examples 1 and 2 and substituting the appropriate starting materials the compounds 3–12 are made.
















 










Example
R
W
Z













3
Me
CH2O






 


4
Et
CH═CH






 


5
Me










 


6
Me
—CH═C═CH—






 


7
Me
CH2S






 


8
Me
CH2O






 


9
Et
CH2O






 


10
Me
CH2CH2






 


11
Me
CH2NH






 


12
H
CH2NMe













Examples 1–12 show that EP1-specific agonists can be synthesized according to this invention.
Example 13
Preparation of 2-decarboxy-2-bromo-11-beta-fluoro-13,14-dihydryo-15-indanyl-15-pentanor-PGF2α11,15-tert-butyldimethylsilylate, 9-acetate (E1b)




In a round bottom flask under argon the Wittig salt (a) (2.2 equiv.) is added to THF, and cooled to −78° C. Sodium hexamethyidisilazide (4.4 equiv.) is then added in one portion and the reaction is stirred for 15 minutes at −78° C. The solution is then warmed to 0° C. for two hours. The reaction mixture is cooled to −78° C. and the lactol E13a in THF is added over 10 minutes.
E13a is prepared from Corey Aldehyde in a manner analogous to that taught in PCT Publication No. WO 98/20880. The solution is stirred at −78° C. for 1 hour and then allowed to warm to room temperature and stirred an additional 17 hours. The reaction mixture is quenched with water and the THF is removed under reduced pressure. The residue is dissolved in EtOAc/hexane and washed two times with 1N HCl. The organic layer is dried with Na2SO4) and solvent is removed under reduced pressure. The residue is dissolved in MeOH and TMS diazomethane (5 equiv.) is added slowly to the mixture. The material is concentrated and it is then chromatographed on SiO2 (10% EtOAc/hexanes) to provide the ester as a yellow oil.
To a solution of the ester (1 equiv.) in THF at −78° C. is added Lithium Aluminum Hydride (LAH) (1.3 equiv.) in one portion. The reaction mixture is stirred for 1 hour at −78° C. and then quenched with water. The solution is poured into CH2Cl2 and the aqueous layer is acidified to pH=1. The aqueous layer is re-extracted with CH2Cl2 and organics are combined. The organic layer is dried (Na2SO4) and concentrated. The residue is chromatographed on SiO2 (10% EtOAc/hexanes) to provide the alcohol as a colorless oil. To a solution of that alcohol and pyridine (1.6 equiv.) in acetonitrile is added, at 0° C., over 10 min solid dibromo-triphenylphosphine (1.4 equiv.). The reaction is monitored by TLC (4% EtOAc/hexanes) until complete. The solution is added to 5% EtOAc/hexanes and then the mixture is chromatographed on SiO2 (5% EtOAc/hexanes) to provide the primary bromide as a colorless oil. This product is dissolved in pyridine and acetic anhydride (1.9 equiv.) and DMAP (0.05 equiv.). The reaction is stirred at 25° C. overnight. The reaction is then quenched with water. The organic layer is washed with brine, dried over MgSO4, and concentrated in vacuo to give a crude product which is purified by flash chromatography on silica gel (hexanes then 1% MeOH in CH2Cl2) to 2-decarboxy-2-bromo-11-beta-fluoro-13,14-dihydryo-15-indanyl-15-pentanor-PGF2α15-tert-butyldimethylsilylate, 9-acetate (E13b).
Example 14
Preparation of 2-decarboxy-2-(P-methylphosphinico)-13,14-dihydryo-15-indanyl-15-pentanor-PGF2a (E14b)




a. 2-decarboxy-2-(O-ethyl-P-methylphosphinico)-13,14-dihydryo-15-indanyl-15-pentanor-PGF2α15-tert-butyldimethylsilylate, 9-acetate (E14a): A mixture of 2-decarboxy-2-bromo-13,14-dihydryo-15-indanyl-15-pentanor-PGF2α15-tert-butyldimethylsilylate 9-acetate, diethyl methylphosphonite (E13b) and toluene is stirred at 100° C. for 8 hours. The toluene is evaporated and the residue is purified by SiO2 column chromatography (5–25% 2-propanol in 20% dichloromethane-hexane). Appropriate fractions are concentrated, diluted with water, and extracted three times with ethyl acetate. The combined extracts are washed with water, are dried (brine, Na2SO4), and are evaporated to give 2-decarboxy-2-(O-ethyl-P-methyl phosphinico)-13,14-dihydryo-15-indanyl-15-pentanor-PGF2α15-tert-butyldimethylsilylate, 9-acetate (E14a).
b. 2-decarboxy-2-(P-methylphosphinico)-13,14-dihydryo-15-indanyl-15-pentanor-PGF2α (E14b): E14a is added to a 2.5M aqueous sodium hydroxide and is stirred at reflux for 3 hours. The mixture is then diluted with water and is washed two times with ethyl acetate. The combined washes are extracted with water. The combined aqueous phases are acidified with 1M aqueous hydrochloric acid and extracted four times with ethyl acetate. The combined extracts are dried (brine, Na2SO4) then re dissolved in acetonitrile and HF/pyridine is added at zero degrees centigrade. The reaction is allowed to warm to room temperature, then the crude is concentrated by rotary evaporation. The residue is chromatographed to yield 2-decarboxy-2-(P-methylphosphinico)-13,14-dihydro-15-indanyl-15-pentanor-PGF2α (E14b).
Examples 15–26
Using largely the procedure set forth in Examples 13 and 14 and substituting the appropriate starting materials the compounds 15–26 are made.
















 










Example
R
a
Z





15
Me
CH2CH2






 


16
Et
CH═CH






 


17
Me










 


18
Me
—CH═C═CH—






 


19
Me
CH2CH2






 


20
Me
CH═CH






 


21
Et
CH═CH






 


22
Me
CH2CH2






 


23
Me
CH═CH






 


24
H
CH═CH






 


25
Me
CH2CH2






 


26
Me
CH═CH













Examples 13–26 show that FP-specific antagonists can be prepared according to this invention.
Example 27
Preparation of 3-(6-iodo)hex-3-enyl)-2-morpholin-4-yl-4-((4-phenyl)phenyl)methoxy)cyclopentan-1-ol TBDMS ether (E27b)




In a round bottom flask under argon the acid (E27a) (1 equiv.) is added to MeOH, and cooled to −10° C. Sodium borohydride (1.5 equiv.) is then added in one portion and the reaction is stirred for 15 minutes at −10° C. The solution is then warmed to room temperature for two hours. Acetic acid is added and the methanol removed under vacuum. The crude material is resuspended in dichloromethane and triethylamine (1.1 equiv.) is added. The solution is stirred at −78° C. while TBDMSTf (1.05 equiv.) is added dropwise. The reaction is stirred for 1 hour and then allowed to warm to room temperature and stirred an additional 1 hour. The reaction mixture is quenched with water and the methylene chloride is removed under reduced pressure. The residue is dissolved along with oxalyl chloride and dimethylformamide in dichloromethane and is allowed to stand at room temperature for 30 minutes and is then concentrated. The residue is taken up in dichloromethane. Separately, a mixture of the sodium salt of N-hydroxypyridine-2-thione, DMAP, and dichloromethane under argon is brought to reflux by irradiation with a 250 Watt General Electric floodlamp. To this mixture is added 1,1,1-trifluoro-2-iodoethane, followed by the solution of acid chloride which is added over 15 minutes. Irradiation is continued for 45 minutes and then the mixture is concentrated and purified by dry-flash column chromatography to give the 1-nor-primary iodide E27b.
Example 28
Preparation of 3-(6-(methyl(hydroxyphosphoryl))hex-3-enyl)-2-morpholin-4-yl-4-((4-phenyl)phenyl)methoxy)cyclopentan-1-one (E28c)




a. Preparation of 3-(6-(methyl(ethoxyphosphoryl))hex-3-enyl)-2-morpholin-4-yl-4-((4-phenyl)phenyl)methoxy)cyclopentan-1-ol; TBDMS ether (E28a): A mixture of E27b and toluene is stirred at 100° C. for 8 hours. The toluene is evaporated and the residue is purified by SiO2 column chromatography (5–25% 2-propanol in 20% dichloromethane-hexane). Appropriate fractions are concentrated, diluted with water, and extracted three times with ethyl acetate. The combined extracts are washed with water, are dried (brine, Na2SO4), and are evaporated to give 3-(6-(methyl(ethoxyphosphoryl))hex-3-enyl)-2-morpholin-4-yl-4-((4-phenyl)phenyl)methoxy)cyclopentan-1-ol; TBDMS ether (E28a):
b. Preparation of 3-(6-(methyl(hydroxyphosphoryl))hex-3-enyl)-2-morpholin-4-yl-4-((4-phenyl)phenyl)methoxy)cyclopentan-1-ol; (E28b): E28a is added to a 2.5M aqueous sodium hydroxide and is stirred at reflux for 3 hours. The mixture is then diluted with water and is washed two times with ethyl acetate. The combined washes are extracted with water. The combined aqueous phases are acidified with 1M aqueous hydrochloric acid and extracted four times with ethyl acetate. The combined extracts are dried (brine, Na2SO4) then re dissolved in acetonitrile and HF/pyridine is added at zero degrees centigrade. The reaction is allowed to warm to room temperature, then the crude is concentrated by rotary evaporation. The residue is chromatographed to 3-(6-methyl)hydroxyphosphoryl))hex-3-enyl)-2-morpholin-4-yl-4-((4-phenyl)phenyl)methoxy)cyclopentan-1-ol; (E28b).
b. Preparation of 3-(6-(methyl(hydroxyphosphoryl))hex-3-enyl)-2-morpholin-4-yl-4-((4-phenyl)phenyl)methoxy)cyclopentan-1-one; (E28c): E28b is added to a solution of dichloromethane and PCC is slowly added along with molecular sieves. The solution is stirred and monitored by TLC until the complete consumption of starting material. The solution is then filtered thru Celite and Fluorosil and purified, if necessary by silica gel chromatography yielding 3-(6-(methyl(hydroxyphosphoryl))hex-3-enyl)-2-morpholin-4-yl-4-((4-phenyl)phenyl)methoxy)cyclopentan-1-one; (E28c):.
Examples 29–35
Using largely the procedure set forth in Examples 27 and 28 and substituting the appropriate starting materials the compounds 29–35, having the formula below, are made.
















 











Example
R
a
R′
R′′





29
Me
CH2CH2
H
H


30
Et
CH═CH
H
3,5-diF


 


31
Me




H
H


 


32
Me
—CH═C═CH—
H
3,5-diF


33
Me
CH2CH2
2-F
H


34
Me
CH═CH
3-F
H


35
Et
CH═CH
2,2′-diF
H









Examples 27–35 show that EP4 antagonists can be synthesized according to this invention.
Example 36
Preparation of 7-methoxy-6-(3-methoxyoctyl)-2-oxabicyclo[3.3.0]octan-3-ol (E36b)




In a round bottom flask under argon the commercially-available Corey lactone (E36a) (available from Cayman Chemical or Aldrich Chemical) is placed in an inert solvent. Dimethyl sulfate (2 equivalents) is added dropwise, and the mixture is then stirred for 15 hours. The solution is then warmed for two hours. The reaction mixture is cooled and the lactone is isolated by chromatography, then cooled to −78° C. for 1 hour and DIBAL is added to effect reduction to the lactol, E36b. The material is concentrated and then taken to the next step as soon as possible without further purification.
Example 37
Preparation of 2-decarboxy-2-bromo-PGF1α11,15-dimethoxy, 9-tert-butyldimethylsilylate, (E37a)




In a round bottom flask under argon the Wittig salt (a) (2.2 equiv.) is added to THF, and cooled to −78° C. Sodium hexamethyldisilazide (4.4 equiv.) is then added in one portion and the reaction is stirred for 15 minutes at −78° C. The solution is then warmed to 0° C. for two hours. The reaction mixture is recooled to −78° C. and the lactol E36b in THF is added over 10 minutes. The solution is stirred at −78° C. for 1 hour and then allowed to warm to room temperature and stirred an additional 17 hours. The reaction mixture is quenched with water and the THF is removed under reduced pressure. The residue is brought up in EtOAc/hexane and washed two times with 1N HCl. The organic layer is dried with Na2SO4 and solvent is removed under reduced pressure. The residue is taken up in MeOH and TMS diazomethane (5 equiv.) is added slowly to the mixture. The solvent is concentrated and the residue is chromatographed on SiO2 (10% EtOAc/hexanes) to provide the ester as a yellow oil.
To a solution of the ester (1 equiv.) in CH2Cl2 is added dropwise at −78° C.: 2,6-lutidine (1.9 equiv.) followed by TBDMSOTf (1.8 equiv.). The reaction is stirred for 30 minutes at −78° C. and then warmed to 25° C. overnight. The reaction is quenched with water. The organic layer is then washed with water, dried over MgSO4, and concentrated in vacuo to give a crude isolate which is subjected to flash chromatography on silica gel eluting with hexanes then 1% MeOH in CH2Cl2 to give the 9-silyl-protected product. This product is dissolved in ethyl acetate and hydrogenated carefully with hydrogen gas over Pd on C (5%). The product is filtered, then the ethyl acetate is removed in vacuo, and to the residue in THF at −78° C. is added Lithium Aluminum Hydride (LAH) (1.3 equiv.) in one portion. The reaction mixture is stirred for 1 hour at −78° C. and then quenched with water. The solution is poured into CH2Cl2 and the aqueous layer is acidified to pH=1. The aqueous layer is re-extracted with CH2Cl2 and organics are combined. The organic layer is dried (Na2SO4) and concentrated. The residue is chromatographed on SiO2 (10% EtOAc/hexanes) to provide the alcohol as a colorless oil. To a solution of that alcohol and pyridine (1.6 equiv.) in acetonitrile is added, at 0° C., over 10 min solid dibromo-triphenylphosphine (1.4 equiv.). The reaction is monitored by TLC (4% EtOAc/hexanes) until complete. The solution is added to 5% EtOAc/hexanes and then the mixture is chromatographed on SiO2 (5% EtOAc/hexanes) to provide the primary bromide as a colorless oil. This product is dissolved in pyridine and acetic anhydride (1.9 equiv.) and DMAP (0.05 equiv.). The reaction is stirred at 25° C. overnight. The reaction is then quenched with water. The organic layer is washed with brine, dried over MgSO4, and concentrated in vacuo to give a crude product which is purified by flash chromatography on silica gel (hexanes then 1% MeOH in CH2Cl2) to give 2-decarboxy-2-bromo-PGF1α11,15-dimethoxy, 9-tert-butyldimethylsilylate, (E37a).
Example 38
Preparation of 2-decarboxy-2-(P-methylphosphinico)-13,14-dihydryo-17-(3,5-difluorophenyl)-17-trinor-PGE2 (E2c)




a. 2-decarboxy-2-(O-ethyl-P-methylphosphinico)-13,14-dihydryo-17-(3,5-difluorophenyl)-17-trinor-PGF2α11,15-bis tert-butyldimethylsilylate, 9-acetate (E38a): A mixture of 2-decarboxy-2-bromo-PGF1α11,15-dimethoxy, 9-tert-butyldimethylsilylate, (E37a), diethyl methylphosphonite, and toluene is stirred at 100° C. for 8 hours. The toluene is evaporated and the residue is purified by SiO2 column chromatography (5–25% 2-propanol in 20% dichloromethane-hexane). Appropriate fractions are concentrated, diluted with water, and extracted three times with ethyl acetate. The combined extracts are washed with water, are dried (brine, Na2SO4), and are evaporated to give 2-decarboxy-2-(O-ethyl-P-methylphosphinico)-PGF1α111,15-dimethoxy, 9-tert-butyldimethylsilylate (E38a).
b. 2-decarboxy-2-(P-methylphosphinico)-11,15-dimethoxy PGF1α9-tert-butyl dimethyl silylate (E38b): E38a is added to a 2.5M aqueous sodium hydroxide and is stirred at reflux for 3 hours. The mixture is then diluted with water and is washed two times with ethyl acetate. The combined washes are extracted with water. The combined aqueous phases are acidified with 1M aqueous hydrochloric acid and extracted four times with ethyl acetate. The combined extracts are dried (brine, Na2SO4) then chromatographed on silica gel to yield 2-decarboxy-2-(P-methylphosphinico)-11,15-dimethoxy PGF1α9-tert-butyl dimethyl silylate (E38b).
c. 2-decarboxy-2-(P-methylphosphinico)-11,15-dimethoxy-PGE2 (E38c): The product E38b is dissolved in acetonitrile and HF/pyridine is added at zero degrees centigrade. The reaction is allowed to warm to room temperature, then the crude is concentrated by rotary evaporation, and the residue is chromatographed to yield the free acohol, which is dissolved in methylene chloride and PCC is added. When the reaction is complete by TLS, the crude mixture is filtered through Fluorosil, and the residue is concentrated to yield 2-decarboxy-2-(P-methylphosphinico)-11,15-dimethoxy-PGE2 (E38c).
Examples 39–52
Using largely the procedure set forth in Examples 36 to 38 and substituting the appropriate starting materials the compounds 39–52 are made.
















 










Example
R
a
Z





39
Me
CH2CH2






 


40
Et
CH═CH






 


41
Me










 


42
Me
—CH═C═CH






 


43
Me
CH2CH2






 


44
Me
CH═CH






 


45
Et
CH═CH






 


46
Me
CH2CH2






 


47
Me
CH2CH2






 


48
H
CH═CH






 


49
Me
CH2CH2






 


50
Me
CH2CH2













Examples 36–50 show that EP3-specific agonists can be synthesized according to this invention. They are made in analogy to Examples 1 and 2 above, or when available, they can be made directly from the commercially-available prostaglandins or intermediates, such as in Example 36.
Example 51
Preparation of (4Z)-6-[(1R,2S,3S,4S)-3-(5-hydroxybenzo[b]thiophen-3-carbonylamino)bicyclo[2.2.1]-4-hexenyl-P-methyl-phosphinic acid (E51B) sodium salt




a. A mixture of (5Z)-7-[(1R,2S,3S,4S)-3-(5-hydroxybenzo[b]thiophen-3-carbonylamino)bicyclo[2.2.1]-5-heptenoic acid (E51A) (available as described in WO99/15502-A1), oxalyl chloride and dimethylformamide in dichloromethane is allowed to stand at room temperature for 30 minutes and is then concentrated. The residue is taken up in dichloromethane. Separately, a mixture of the sodium salt of N-hydroxypyridine-2-thione, DMAP, and dichloromethane under argon is brought to reflux by irradiation with a 250 Watt General Electric floodlamp. To this mixture is added 1,1,1-trifluoro-2-iodoethane, followed by the solution of acid chloride which is added over 15 minutes. Irradiation is continued for 45 minutes and then the mixture is concentrated and purified by dry-flash column chromatography to give the 1-nor-primary iodide
A mixture of the iodide, diethyl methylphosphonite, and toluene is stirred at 100° C. for 7 hours. The solvents are evaporated and the residue is purified by SiO2 column chromatography (5–25% 2-propanol in 20% dichloromethane-hexane). Appropriate fractions are concentrated, diluted with water and extracted with ethyl acetate three times. The combined extracts are washed with water, dried (brine, Na2SO4), and evaporated to yield the phosphinic acid ester.
A mixture of the phosphinic acid ester, 95% ethanol, and 2.5M aqueous sodium hydroxide is stirred at reflux for 3 hours. The mixture is diluted with water and is washed with ethyl acetate two times. The combined washes are extracted with water. The combined aqueous phases are acidified with 1M aqueous hydrochloric acid and extracted with ethyl acetate four times. The combined extracts are dried (brine, Na2SO4) and are evaporated. The residue is taken up in methanol and stirred with Amberlite CG-50 (Na+ form) for 15 minutes. The resin is removed by filtration and the filtrate is concentrated to give (4Z)-6-[(1R,2S,3S,4S)-3-(5-hydroxybenzo[b]thiophen-3-carbonylamino)bicyclo[2.2.1]-4-hexenyl-P-methyl-phosphinic acid sodium salt, (E51b).
Examples 52–60
Examples 52–60 are prepared using substantially the same procedure as that described in Example 51, substituting the appropriate starting materials. The skilled artisan may change temperature, pressure, atmosphere, solvents or the order of reactions as appropriate. Additionally, the skilled artisan may use protecting groups to block side reactions or increase yields as appropriate. All such modifications can readily be carried out by the skilled artisan in the art of organic chemistry, and thus are within the scope of the invention.
Examples 52–60
















 










Example
R1
X
R2





52
Me
C═O






 


53
Et
SO2






 


54
Me
SO2






 


55
iPr
C═O






 


56
Me
SO2






 


57
Me
C═O






 


58
Et
SO2






 


59
Pr
SO2






 


60
Me
C═O













For some structures, it is advantageous to construct a complete prostaglandin using known means and then transform the completed prostaglandin into the phosphinic acid using substantially the same means as Example 51. The skilled artisan will recognize when interfering functionality would make it desirable to do so.
Examples 51–60 show that TP antagonists can be prepared according to this invention. The TP-specific antagonists are made in analogy to examples 1 and 2 above, or when available, they can be made directly from the commercially-available prostaglandins or intermediates, such as in Example 51.
Example 61
Preparation of (E61b)




E61b: To a solution of butaprost (E61a) (1 equiv.) in CH2Cl2 at −78° C. is added 2,6 lutidine (2.3 equiv.) dropwise over 15 minutes. The solution is kept at 0° C., and TBDMS Triflate (2.2 equiv.) in CH2Cl2 is added dropwise over 15 minutes. The reaction is warmed gradually to room temperature and stirred for 15 hours. Aqueous 1N HCl is added and the layers are quickly separated. The water layer is extracted once with CH2Cl2 and the organic layers are combined. The combined organic layer is washed with brine, dried (over solid Na2SO4) and concentrated. The residue is chromatographed on SiO2 (5% EtOAc/hexanes) to provide the bis-silylated ester as an oil. To a solution of this oil (1 equiv.) in methanol at 0° C. is added Sodium Borohydride (NaBH4) (1.3 equiv.) in one portion. The reaction mixture is stirred for 1 hour at 0° C. and then concentrated, and the concentrate partitioned between water and ethyl acetate (EtOAc). The aqueous layer is re-extracted once with EtOAc and the organics are combined. The organic layer is then dried (Na2SO4) and concentrated. The residue is chromatographed on silica gel (10% EtOAc/hexanes) to provide a mixture of C-9 alcohols as a colorless oil. This mixture is dissolved in pyridine and acetic anhydride (1.8 equiv.) and DMAP (0.05 equiv.). The reaction is stirred at 25° C. overnight. The reaction is then quenched with water. The organic layer is washed with brine, dried over MgSO4, and concentrated in vacuo to give a crude product which is purified by flash chromatography on silica gel (hexanes then 1% MeOH in CH2Cl2) to give the bis-t-butyldimethylsilylate, 9-acetate (E61b).
Example 62
Preparation of 2-nor-2-P-methylphosphinico butaprost (E62b)




a. A mixture of protected, reduced butaprost (E61b); oxalyl chloride and dimethylformamide in dichloromethane is allowed to stand at room temperature for 30 minutes and is then concentrated. The residue is taken up in dichloromethane. Separately, a mixture of the sodium salt of N-hydroxypyridine-2-thione, DMAP, and dichloromethane under argon is brought to reflux by irradiation with a 250 Watt General Electric floodlamp. To this mixture is added 1,1,1-trifluoro-2-iodoethane, followed by the solution of acid chloride which is added over 15 minutes. Irradiation is continued for 45 minutes and then the mixture is concentrated and purified by dry-flash column chromatography to give the 1-nor-primary iodide
A mixture of the iodide, diethyl methylphosphonite, and toluene is stirred at 100° C. for 7 hours. The solvents are evaporated and the residue is purified by SiO2 column chromatography (5–25% 2-propanol in 20% dichloromethane-hexane). Appropriate fractions are concentrated, diluted with water and extracted with ethyl acetate three times. The combined extracts are washed with water, dried (brine, Na2SO4), and evaporated to yield the phosphinic acid ester.
A mixture of the phosphinic acid ester, 95% ethanol, and 2.5M aqueous sodium hydroxide is stirred at reflux for 3 hours. The mixture is diluted with water and is washed with ethyl acetate two times. The combined washes are extracted with water. The combined aqueous phases are acidified with 1M aqueous hydrochloric acid and extracted with ethyl acetate four times. The combined extracts are dried (brine, Na2SO4) and are evaporated. The residue is taken up in methanol and stirred with Amberlite CG-50 (Na+ form) for 15 minutes. The resin is removed by filtration and the filtrate is brought to a pH=3.0, then concentrated to give 5-(4-t-butyldimethylsilyloxy-4-(1-propylcyclobutyl)but-1-enyl)4-(6-(P-methyl(hydroxyphosphoryl))hexyl)3-t-butyldimethylsilyloxycyclopentan-1-ol (E62a).
Example 63
Preparation of E63a




e. 5-(3-hydroxy-5-(2-fluorophenyl)pent-1-enyl)-4-(6-(methyl(hydroxyphosphoryl))hex-2-enyl)cyclopentane-1,3-diol, sodium salt (E1f): The compound E62a is dissolved in methylene chloride and PCC is added. The reaction is monitored by TLC and when the starting material is consumed, the reaction is poured through a plug of silica and Fluorosil, then concentrated. Without further purification to the crude reaction mixture of the 2-decarboxy-2-(O-ethyl-P-methylphosphinico)prostaglandin derivative acetonitrile and 1.1 equivalents of HF/Pyridine are added while the flask is kept at 0° C. After 3 hours at 0° C., the reaction is quenched with saturated aqueous NaCl. The aqueous layer is extracted three times with CH2Cl2. The organic layers are combined and washed three times with 1N HCl, brine, and dried over sodium sulfate. After column chromatography, (7:3, Hexane: ethyl acetate) the silyl-free product is obtained (E63a).
Examples 64–75
Using largely the procedure set forth in Examples 61 to 63 and substituting the appropriate starting materials the compounds 64–75 are made.











Ex-



am-


ple
Structure







64






 


65






 


66






 


67






 


68






 


69






 


70






 


71






 


72






 


73






 


74






 


75













Examples 61–75 show that EP2-specific agonists can be prepared according to this invention. They are made in analogy to Examples 1 and 2 above, or when available, they can be made directly from the commercially-available prostaglandins or intermediates, such as in Example 61.
Example 76
Preparation of 2-decarboxy-2-bromo-17-(3-methoxymethylphenyl)-17-trinor-PGF2α11,15-bis tert-butyldimethylsilylate, 9-acetate (E76b)




In a round bottom flask under argon the Wittig salt (a) (2.2 equiv.) is added to THF, and cooled to −78° C. Sodium hexamethyldisilazide (4.4 equiv.) is then added in one portion and the reaction is stirred for 15 minutes at −78° C. The solution is then warmed to 0° C. for two hours. The reaction mixture is then cooled again to −78° C. and the lactol E76a in THF is added over 10 minutes. E76a is prepared from Corey Aldehyde in a manner analogous to that taught in U.S. Pat. No. 6,048,895. The solution is stirred at −78° C. for 1 hour and then allowed to warm to room temperature and stirred an additional 17 hours. The reaction mixture is quenched with water and the THF is removed under reduced pressure. The residue is brought up in EtOAc/hexane and washed twice with 1N HCl. The organic layer is dried with (Na2SO4) and solvent is removed under reduced pressure. The residue is taken up in MeOH and TMS diazomethane (5 equiv.) is added slowly to the mixture. The solvent is concentrated and the residue is chromatographed on SiO2 (10% EtOAc/hexanes) to provide the ester as a yellow oil.
To a solution of the ester (1 equiv.) in THF at −78° C. is added Lithium Aluminum Hydride (LAH) (1.3 equiv.) in one portion. The reaction mixture is stirred for 1 hour at −78° C. and then quenched with water. The solution is poured into CH2Cl2 and the aqueous layer is acidified to pH=1. The aqueous layer is re-extracted with CH2Cl2 and organics are combined. The organic layer is dried (Na2SO4) and concentrated. The residue is chromatographed on SiO2 (10% EtOAc/hexanes) to provide the alcohol as a colorless oil. To a solution of that alcohol and pyridine (1.6 equiv.) in acetonitrile is added, at 0° C., over 10 min solid dibromo-triphenylphosphine (1.4 equiv.). The reaction is monitored by TLC (4% EtOAc/hexanes) until complete. The solution is added to 5% EtOAc/hexanes and then the mixture is chromatographed on SiO2 (5% EtOAc/hexanes) to provide the primary bromide as a colorless oil. This product is dissolved in pyridine and acetic anhydride (1.9 equiv.) and DMAP (0.05 equiv.). The reaction is stirred at 25° C. overnight. The reaction is then quenched with water. The organic layer is washed with brine, dried over MgSO4, and concentrated in vacuo to give a crude product which is purified by flash chromatography on silica gel (hexanes then 1% MeOH in CH2Cl2) to 2-decarboxy-2-bromo-17-(3-methoxymethylphenyl)-17-trinor-PGF2α11,15-bis tert-butyldimethylsilylate, 9-acetate (E76b).
Example 77
Preparation of 2-decarboxy-2-(P-methylphosphinico)-17-(3-methoxymethylphenyl)-17-trinor-PGE1 (E77c)




a. 2-decarboxy-2-(O-ethyl-P-methylphosphinico)-17-(3-methoxy-methylphenyl)-17-trinor-PGF2α11,15-bis tert-butyldimethylsilylate, 9-acetate (E77a): A mixture of 2-decarboxy-2-bromo-17-(3-methoxymethylphenyl)-17-trinor-PGF2α11,15-bis tert-butyldimethylsilylate, 9-acetate, diethyl methylphosphonite (E76b) and toluene is stirred at 100° C. for 8 hours. The toluene is evaporated and the residue is purified by SiO2 column chromatography (5–25% 2-propanol in 20% dichloromethane-hexane). Appropriate fractions are concentrated, diluted with water, and extracted three times with ethyl acetate. The combined extracts are washed with water, are dried (brine, Na2SO4), and are evaporated to give 2-decarboxy-2-(O-ethyl-P-methylphosphinico)-17-(3-methoxy methylphenyl)-17-trinor-PGF2α11,15-bis tert-butyldimethylsilylate, 9-acetate (E77a).
b. 2-decarboxy-2-(P-methylphosphinico)-17-(3-methoxymethylphenyl)-17-trinor-PGF1α11,15-bis tert-butyldimethylsilylate (E77b): The compound E77a is added to a flask in ethyl acetate and Pd/C 5% is added. This is stirred under an atmosphere of H2 gas until gas uptake ceases, then filtered and the solvent removed in vacuuo. The residue is added to a 2.5M aqueous sodium hydroxide and is stirred at reflux for 3 hours. The mixture is then diluted with water and is washed two times with ethyl acetate. The combined washes are extracted with water. The combined aqueous phases are acidified with 1M aqueous hydrochloric acid and extracted four times with ethyl acetate. The combined extracts are dried (brine, Na2SO4) then dissolved in methylene chloride and pyridinium chlorochromate is added to effect oxidation of C-9 to the ketone. After three hours, the solution is filtered through silica, and the solvent is evaporated to give 2-decarboxy-2-(P-methylphosphinico)-17-(3-methoxymethylphenyl)-17-trinor-PGE111,15-bis tert-butyldimethylsilylate (E77b).
c. 2-decarboxy-2-(P-methylphosphinico)-17-(3-methoxymethylphenyl)-17-trinor-PGE1 (E77c): The product E77b is dissolved in acetonitrile and HF/pyridine is added at zero degrees centigrade. The reaction is allowed to warm to room temperature, then the crude is concentrated by rotary evaporation, and the residue is chromatographed to yield 2-decarboxy-2-(P-methylphosphinico)-17-(3-methoxymethylphenyl)-17-trinor-PGE1 (E77c).
Using largely the procedure set forth in Examples 76 and 77 and substituting the starting materials the compounds 78–85 are made.
















 













Example
R
A
A′
A′′
W
Z





78
Me
CH2
S
CH2
CH2






 


79
Et
S
CH2
S
CH2






 


80
Me
S
CH2
S
CH2






 


81
Me
S
CH2
S
CH2






 


82
Me
S
CH2
S
CH(CH3)






 


83
Me
CH2
S
CH2
CH2O






 


84
Et
CH2
S
CH2
CH2






 


85
Me
CH2
S
CH2
CH2CH2













Example 86
Preparation of [1α(Z),2β,5α]-7-{5-([1,1′-biphenyl]-4-yl methoxy)-2-(4-morpholinyl)-3-oxocyclopentyl]-4-heptenyl P-methyl phosphinic acid sodium salt (E86b)




A mixture of [1α(Z),2β,5α]-7-{5-([1,1′-biphenyl]-4-yl methoxy)-2-(4-morpholinyl)-3-oxocyclopentyl]4-heptenoic acid (E86a) (available from Cayman Chemical Company), (or its reduced 3-alcohol), oxalyl chloride and dimethylformamide in dichloromethane is allowed to stand at room temperature for 30 minutes and is then concentrated. The residue is taken up in dichloromethane. Separately, a mixture of the sodium salt of N-hydroxypyridine-2-thione, DMAP, and dichloromethane under argon is brought to reflux by irradiation with a 250 Watt General Electric floodlamp. To this mixture is added 1,1,1-trifluoro-2-iodoethane, followed by the solution of acid chloride which is added over 15 minutes. Irradiation is continued for 45 minutes and then the mixture is concentrated and purified by dry-flash column chromatography to give the 1-nor-primary iodide
A mixture of the iodide, diethyl methylphosphonite, and toluene is stirred at 100° C. for 7 hours. The solvents are evaporated and the residue is purified by SiO2 column chromatography (5–25% 2-propanol in 20% dichloromethane-hexane). Appropriate fractions are concentrated, diluted with water and extracted with ethyl acetate three times. The combined extracts are washed with water, dried (brine, Na2SO4), and evaporated to yield the phosphinic acid ester.
A mixture of the phosphinic acid ester, 95% ethanol, and 2.5M aqueous sodium hydroxide is stirred at reflux for 3 hours. The mixture is diluted with water and is washed with ethyl acetate two times. The combined washes are extracted with water. The combined aqueous phases are acidified with 1M aqueous hydrochloric acid and extracted with ethyl acetate four times. The combined extracts are dried (brine, Na2SO4) and are evaporated. If the free alcohol is used it is oxidized with PCC to the ketone at this point. The residue is taken up in methanol and stirred with Amberlite CG-50 (Na+ form) for 15 minutes. The resin is removed by filtration and the filtrate is concentrated to give [1α(Z),2β,5α]-7-{5-([1,1′-biphenyl]-4-yl methoxy)-2-(4-morpholinyl)-3-oxocyclopentyl]-4-heptenyl P-methyl phosphinic acid sodium salt, (E86b).
Example 87
Preparation of [1α(Z),2β,5α]-7-{5-([1,1′-biphenyl]4-yl methoxy)-2-(4-morpholinyl)-3-oxocyclopentyl]-heptanyl P-ethyl phosphinic acid sodium salt, (E87)




Examples 76–87 show that EP4 agonists can be synthesized according to this invention.
Example 88
Preparation of 1-(3-cyclohexyl-3-hydroxypropyl)-5-(6-(methyl(hydroxy-phosphoryl))hexyl)imiddazolidine-2,4-dione (E88b)




A mixture of (4S)-(3-[3R,S)-3-cyclohexyl-3-hydroxypropyl]-2,5-dioxo)-4-imidazolidineheptanoic acid (E88A) (available from Cayman Chemical Company), oxalyl chloride and dimethylformamide in dichloromethane is allowed to stand at room temperature for 30 minutes and is then concentrated. The residue is taken up in dichloromethane. Separately, a mixture of the sodium salt of N-hydroxypyridine-2-thione, DMAP, and dichloromethane under argon is brought to reflux by irradiation with a 250 Watt General Electric floodlamp. To this mixture is added 1,1,1-trifluoro-2-iodoethane, followed by the solution of acid chloride which is added over 15 minutes. Irradiation is continued for 45 minutes and then the mixture is concentrated and purified by dry-flash column chromatography to give the 1-nor-primary iodide
A mixture of the iodide, diethyl methylphosphonite, and toluene is stirred at 100° C. for 7 hours. The solvents are evaporated and the residue is purified by SiO2 column chromatography (5–25% 2-propanol in 20% dichloromethane-hexane). Appropriate fractions are concentrated, diluted with water and extracted with ethyl acetate three times. The combined extracts are washed with water, dried (brine, Na2SO4), and evaporated to yield the phosphinic acid ester.
A mixture of the phosphinic acid ester, 95% ethanol, and 2.5M aqueous sodium hydroxide is stirred at reflux for 3 hours. The mixture is diluted with water and is washed with ethyl acetate two times. The combined washes are extracted with water. The combined aqueous phases are acidified with 1M aqueous hydrochloric acid and extracted with ethyl acetate four times. The combined extracts are dried (brine, Na2SO4) and are evaporated. The residue is taken up in methanol and stirred with Amberlite CG-50 (Na+ form) for 15 minutes. The resin is removed by filtration and the filtrate is concentrated to give 1-(3-cyclohexyl-3-hydroxypropyl)-5-(6-(methyl(hydroxy-phosphoryl))hexyl)imiddazolidine-2,4-dione sodium salt, (E88b).
Examples 89–90
Examples 89–90 are prepared using substantially the same procedure as that described in Example 88, substituting the appropriate starting materials. The skilled artisan may change temperature, pressure, atmosphere, solvents or the order of reactions as appropriate. Additionally, the skilled artisan may use protecting groups to block side reactions or increase yields as appropriate. All such modifications can readily be carried out by the skilled artisan in the art of organic chemistry, and thus are within the scope of the invention.
Example 89




Example 90




Example 91
Preparation of 7-[3β-[(1,1′-biphenyl]-4-sulfonyl)amino]1α,4α-bicyclo[2.2.1]hept-2α-yl-5Z-heptenyl-P-methyl-phosphinic acid (E91b) sodium salt




A mixture of 7-[3β-[(1,1′-biphenyl]-4-sulfonyl)amino]1α,4α-bicyclo[2.2.1]hept-2α-yl-5Z-heptenoic acid, (E91a) (available from Cayman Chemical Company) oxalyl chloride and dimethylformamide in dichloromethane is allowed to stand at room temperature for 30 minutes and is then concentrated. The residue is taken up in dichloromethane. Separately, a mixture of the sodium salt of N-hydroxypyridine-2-thione, DMAP, and dichloromethane under argon is brought to reflux by irradiation with a 250 Watt General Electric floodlamp. To this mixture is added 1,1,1-trifluoro-2-iodoethane, followed by the solution of acid chloride which is added over 15 minutes. Irradiation is continued for 45 minutes and then the mixture is concentrated and purified by dry-flash column chromatography to give the 1-nor-primary iodide
A mixture of that iodide, diethyl methylphosphonite, and toluene is stirred at 100° C. for 7 hours. The solvents are evaporated and the residue is purified by SiO2 column chromatography (5–25% 2-propanol in 20% dichloromethane-hexane). Appropriate fractions are concentrated, diluted with water and extracted with ethyl acetate three times. The combined extracts are washed with water, dried (brine, Na2SO4), and evaporated to yield the phosphinic acid ester.
A mixture of the phosphinic acid ester, 95% ethanol, and 2.5M aqueous sodium hydroxide is stirred at reflux for 3 hours. The mixture is diluted with water and is washed with ethyl acetate two times. The combined washes are extracted with water. The combined aqueous phases are acidified with 1M aqueous hydrochloric acid and extracted with ethyl acetate four times. The combined extracts are dried (brine, Na2SO4) and are evaporated. The residue is taken up in methanol and stirred with Amberlite CG-50 (Na+ form) for 15 minutes. The resin is removed by filtration and the filtrate is concentrated to give 7-[3β-[(1,1′-biphenyl]-4-sulfonyl)amino]1α,4α-bicyclo[2.2.1]hept-2α-yl-5Z-heptenyl-P-methyl-phosphinic acid sodium salt, (E91b).
Examples 92–94
Examples 92–94 are prepared using substantially the same procedure as that described in Example 91, substituting the appropriate starting materials. The skilled artisan may change temperature, pressure, atmosphere, solvents or the order of reactions as appropriate. Additionally, the skilled artisan may use protecting groups to block side reactions or increase yields as appropriate. All such modifications can readily be carried out by the skilled artisan in the art of organic chemistry, and thus are within the scope of the invention.
Example 92–94











Example
Structure







92






 


93






 


94













Example 95
Preparation of 3-(3-hydroxyoctyl)-2-(6-(methyl(hydroxyphosphoryl))hexyl)-1,3-thiazolidin-4-one (E95B) sodium salt




A mixture of 3-(3-hydroxyoctyl)-2-(carboxyheptyl)-1,3-thiazolidin-4-one (E95a), oxalyl chloride and dimethylformamide in dichloromethane is allowed to stand at room temperature for 30 minutes and is then concentrated. The residue is taken up in dichloromethane. Separately, a mixture of the sodium salt of N-hydroxypyridine-2-thione, DMAP, and dichloromethane under argon is brought to reflux by irradiation with a 250 Watt General Electric floodlamp. To this mixture is added 1,1,1-trifluoro-2-iodoethane, followed by the solution of acid chloride which is added over 15 minutes. Irradiation is continued for 45 minutes and then the mixture is concentrated and purified by dry-flash column chromatography to give the 1-nor-primary iodide
A mixture of the iodide, diethyl methylphosphonite, and toluene is stirred at 100° C. for 7 hours. The solvents are evaporated and the residue is purified by SiO2 column chromatography (5–25% 2-propanol in 20% dichloromethane-hexane). Appropriate fractions are concentrated, diluted with water and extracted with ethyl acetate three times. The combined extracts are washed with water, dried (brine, Na2SO4), and evaporated to yield the phosphinic acid ester.
A mixture of the phosphinic acid ester, 95% ethanol, and 2.5M aqueous sodium hydroxide is stirred at reflux for 3 hours. The mixture is diluted with water and is washed with ethyl acetate two times. The combined washes are extracted with water. The combined aqueous phases are acidified with 1M aqueous hydrochloric acid and extracted with ethyl acetate four times. The combined extracts are dried (brine, Na2SO4) and are evaporated. The residue is taken up in methanol and stirred with Amberlite CG-50 (Na+ form) for 15 minutes. The resin is removed by filtration and the filtrate is concentrated to 3-(3-hydroxyoctyl)-2-(6-(methyl(hydroxy-phosphoryl))hexyl)-1,3-thiazolidin-4-one sodium salt (E95b).
Examples 96–99
Examples 96–99 are prepared using substantially the same procedure as that described in Example 95, substituting the appropriate starting materials. The skilled artisan may change temperature, pressure, atmosphere, solvents or the order of reactions as appropriate. Additionally, the skilled artisan may use protecting groups to block side reactions or increase yields as appropriate. All such modifications can readily be carried out by the skilled artisan in the art of organic chemistry, and thus are within the scope of the invention.
Example 96–99











Exam-



ple
Structure







96






 


97






 


98






 


99













Examples 88–97 show that DP agonists can be prepared according to this invention. The DP agonists are made in analogy to Examples 1 and 2 above, or when available, they can be made directly from the commercially-available prostaglandins or intermediates, such as in Example 88.
Example 100
Preparation of 2-(2-(hydroxycyclohexyl)vinyl)-7-(4-(methyl(hydroxyphosphoryl))butylidene)bicyclo[3.3.0]octan-3-ol (E100b)




A mixture of 9,15-diacetyl, 13,14 dehydro-15-cyclohexyl carbaprostacyclin (E100a) (prepared from 13,14 dehydro-15-cyclohexyl carbaprostacyclin, which is available from Cayman Chemical Company), oxalyl chloride and dimethylformamide in dichloromethane is allowed to stand at room temperature for 30 minutes and is then concentrated. The residue is taken up in dichloromethane. Separately, a mixture of the sodium salt of N-hydroxypyridine-2-thione, DMAP, and dichloromethane under argon is brought to reflux by irradiation with a 250 Watt General Electric floodlamp. To this mixture is added 1,1,1-trifluoro-2-iodoethane, followed by the solution of acid chloride which is added over 15 minutes. Irradiation is continued for 45 minutes and then the mixture is concentrated and purified by dry-flash column chromatography to give the 1-nor-primary iodide
A mixture of the iodide, diethyl methylphosphonite, and toluene is stirred at 100° C. for 7 hours. The solvents are evaporated and the residue is purified by SiO2 column chromatography (5–25% 2-propanol in 20% dichloromethane-hexane). Appropriate fractions are concentrated, diluted with water and extracted with ethyl acetate three times. The combined extracts are washed with water, dried (brine, Na2SO4), and evaporated to yield the phosphinic acid ester.
A mixture of the phosphinic acid ester, 95% ethanol, and 2.5M aqueous sodium hydroxide is stirred at reflux for 3 hours. The mixture is diluted with water and is washed with ethyl acetate two times. The combined washes are extracted with water. The combined aqueous phases are acidified with 1M aqueous hydrochloric acid and extracted with ethyl acetate four times. The combined extracts are dried (brine, Na2SO4) and are evaporated. The residue is taken up in methanol and stirred with Amberlite CG-50 (Na+ form) for 15 minutes. The resin is removed by filtration and the filtrate is concentrated to give 2-(2-(hydroxycyclohexyl)vinyl)-7-(4-(methyl(hydroxyphosphoryl))butylidene)bicyclo[3.3.0]octan-3-ol sodium salt, (E100b).
Example 101–107
Examples 101–107 are prepared using substantially the same procedure as that described in Example 100, substituting the appropriate starting materials, such as ones available from the Cayman Chemical company. The skilled artisan may change temperature, pressure, atmosphere, solvents or the order of reactions as appropriate. Additionally, the skilled artisan may use protecting groups to block side reactions or increase yields as appropriate. All such modifications can readily be carried out by the skilled artisan in the art of organic chemistry, and thus are within the scope of the invention.
Examples 100–107











Example
Structure







101






 


102






 


103






 


104






 


105






 


106






 


107













Examples 100–107 are of the syntheses of IP-specific agonists. They are made in analogy to Examples 1 and 2 above, or when available, they can be made directly from the commercially-available prostaglandins or intermediates, such as in Example 100.
Reference Example 1
Radioligand Binding Assay
IC50 of a prostaglandin derivative can be determined relative to a naturally occurring prostaglandin using the Radioligand Binding Assay. As a control, the IC50 for the naturallly occurring prostaglandin itself should be no lower than 1.0 nM and no higher than 50 nM.
In a typical assay, COS-7 cells are transiently transfected with the hFP recombinant plasmid using LipofectAMINE Reagent. Forty-eight hours later, the tranfected cells are washed with Hank's Balanced Salt Solution (HBSS, without CaCl2, MgCl2, MgSO4, or phenol red). The cells are detached with versene, and HBSS is added. The mixture is centrifuged at 200 g for 10 minutes, at 4° C. to pellet the cells. The pellet is resuspended in Phosphate-Buffered Saline-EDTA buffer (PBS; 1 mM EDTA; pH 7.4; 4° C.). The cells are disrupted by nitrogen cavitation (Parr model 4639), at 800 psi, for 15 minutes at 4° C. The mixture is centrifuged at 1000 g for 10 minutes at 4° C. The supernatant is centrifuged at 100,000 g for 60 minutes at 4° C. The pellet is resuspended to 1 mg protein/mL TME buffer (50 mM Tris; 10 mM MgCl2; 1 mM EDTA; pH 6.0; 4° C.) based on protein levels measured using the Pierce BCA Protein Assay kit. The homogenate is mixed for 10 seconds using a Kinematica POLYTRON® (available from KINEMATICA AG, Luzernerstrasse147A CH-6014 Littau, Switzerland. The membrane preparations are then stored at −80° C., until thawed for assay use.
The receptor competition binding assays are developed in a 96 well format. Each well contains 100 g of hFP membrane, 5 nM (3H) PGF2α, and the various competing compounds in a total volume of 200 L. The plates are incubated at 23° C. for 1 hour. The incubation is terminated by rapid filtration using the Packard Filtermate 196 harvester through Packard UNIFILTER® GF/B filters (available from Packard Instrument Co., Inc. of Downers Grove Ill.) pre-wetted with TME buffer. The filter is washed four times with TME buffer. Packard Microscint 20, a high efficiency liquid scintillation cocktail, is added to the filter plate wells and the plates remain at room temperature for three hours prior to counting. The plates are read on a Packard TOPCOUNT® Microplate Scintillation Counter (also available from Packard Instrument Co., Inc.)
Reference Example 2
Ovariectomized Rat Assay (EP1, 2, 3, 4, Agonists)
Bone activity of the prostaglandins can be conveniently demonstrated using an assay designed to test the ability of the prostaglandins to increase bone volume, mass, or density. An example of such assays is the ovariectomized rat assay.
In the ovariectomized rat assay, six-month old rats are ovariectomized, aged 2 months, and then dosed once a day subcutaneously with a prostaglandin. Upon completion of the study, bone mass and/or density can be measured by dual energy x-ray absorptometry (DXA) or peripheral quantitative computed tomography (pQCT), or micro computed tomography (mCT). Alternatively, static and dynamic histomorphometry can be used to measure the increase in bone volume or formation.
Example 108
Pharmaceutical compositions in the form of tablets are prepared by conventional methods, such as mixing and direct compaction, formulated as follows:














Ingredient
Quantity (mg per tablet)



















Prostaglandin Derivative
5



Microcrystalline Cellulose
100



Sodium Starch Glycollate
30



Magnesium Stearate
3










An EP1 agonist according to this invention is used as the prostaglandin derivative. When administered orally once daily, the above composition substantially increases bone volume in a patient suffering from osteoporosis.
Example 109
Example 108 is repeated using a EP2 agonist according to this invention instead of the EP1 agonist. When administered orally once daily, the above composition substantially increases bone volume in a patient suffering from osteoporosis.
Example 110
Pharmaceutical compositions in liquid form are prepared by conventional methods, formulated as follows:














Ingredient
Quantity




















Prostaglandin Derivative
1
mg



Phosphate buffered physiological saline
10
ml



Methyl Paraben
0.05
ml










An EP3 agonist according to this invention is used as the prostaglandin derivative. When 1.0 ml of the above composition is administered subcutaneously once daily, the above composition substantially increases bone volume in a patient suffering from osteoporosis.
Example 111
Example 110 is repeated using a EP4 agonist according to this invention instead of the EP3 agonist. When administered orally once daily, the above composition substantially increases bone volume in a patient suffering from osteoporosis.
Reference Example 3
Pharmacological Activity for Glaucoma Assay
Pharmacological activity for glaucoma can be demonstrated using assays designed to test the ability of the subject compounds to decrease intraocular pressure. Examples of such assays are described in the following reference, incorporated herein: C. Iiljebris, G. Selen, B. Resul, J. Sternschantz, and U. Hacksell, “Derivatives of 17-Phenyl-18,19,20-trinorprostaglandin F2α Isopropyl Ester: Potential Antiglaucoma Agents”, Journal of Medicinal Chemistry, Vol. 38 No. 2 (1995), pp. 289–304.
Example 112
Topical pharmaceutical compositions for lowering intraocular pressure are prepared by conventional methods and formulated as follows:














Ingredient
Amount (wt %)



















Prostaglandin Derivative
0.004



Dextran 70
0.1



Hydroxypropyl methylcellulose
0.3



Sodium Chloride
0.77



Potassium chloride
0.12



Disodium EDTA (Edetate disodium)
0.05



Benzalkonium chloride
0.01



HCl and/or NaOH
pH 7.2–7.5



Purified water
q.s. to 100%










An EP2 antagonist according to this invention is used as the prostaglandin derivative. When the composition is topically administered to the eyes once daily, the above composition decreases intraocular pressure in a patient suffering from glaucoma.
Example 113
Example 112 is repeated using an EP4 antagonist according to this invention instead of the EP2 antagonist. When administered orally once daily, the above composition substantially increases bone volume in a patient suffering from osteoporosis.
Reference Example 4
Telogen Conversion Assay
Prostaglandins are tested for their potential to grow hair using the Telogen Conversion Assay. The Telogen Conversion Assay measures the potential of a prostaglandin to convert mice in the resting stage of the hair growth cycle (“telogen”), to the growth stage of the hair growth cycle (“anagen”).
Without intending to be limited by theory, there are three principal phases of the hair growth cycle: anagen, catagen, and telogen. It is believed that there is a longer telogen period in C3H mice (Harlan Sprague Dawley, Inc., Indianapolis, Ind.) from approximately 40 days of age until about 75 days of age, when hair growth is synchronized. It is believed that after 75 days of age, hair growth is no longer synchronized. Wherein about 40 day-old mice with dark fur (brown or black) are used in hair growth experiments, melanogenesis occurs along with hair (fur) growth wherein the topical application of hair growth inducers are evaluated. The Telogen Conversion Assay herein is used to screen prostaglandins for potential hair growth by measuring melanogenesis.
Three groups of 44 day-old C3H mice are used: a vehicle control group, a positive control group, and a test prostaglandin group, wherein the test prostaglandin group is administered a prostaglandin used in the method of this invention. The length of the assay is 24 days with 15 treatment days (wherein the treatment days occur Mondays through Fridays). Day 1 is the first day of treatment. A typical study design is shown in Table 4 below. Typical dosage concentrations are set forth in Table RE1, however the skilled artisan will readily understand that such concentrations may be modified.







TABLE RE1







Assay Parameters












Group
Animal

Concen-
Application
Length


#
#
Compound
tration
volume
of Study





1
 1–10
Test
0.01% in
400 μL topical
26 days




Compound
vehicle**


2
11–20
Positive
0.01% in
400 μL topical
26 days




Control
vehicle**




(T3)*


3
21–30
Vehicle**
N/A
400 μL topical
26 days





*T3 is 3,5,3′-triiodothyronine.


**The vehicle is 60% ethanol, 20% propylene glycol, and 20% dimethyl isosorbide (commercially available from Sigma Chemical Co., St. Louis, MO).






The mice are treated topically Monday through Friday on their lower back (base of tail to the lower rib). A pipettor and tip are used to deliver 400 μL to each mouse's back. The 400 μL application is applied slowly while moving hair on the mouse to allow the application to reach the skin.
While each treatment is being applied to the mouse topically, a visual grade of from 0 to 4 will be given to the skin color in the application area of each animal. As a mouse converts from telogen to anagen, its skin color will become more bluish-black. As indicated in Table RE2, the grades 0 to 4 represent the following visual observations as the skin progresses from white to bluish-black.







TABLE RE2







Evaluation Criteria








Visual Observation
Grade





Whitish Skin Color
0


Skin is light gray (indication of initiation of anagen)
1


Appearance of Blue Spots
2


Blue Spots are aggregating to form one large blue area
3


Skin is dark blue (almost black) with color covering majority of
4


treatment area (indication of mouse in full anagen)









Example 114
Compositions for topical administration are made, comprising:














Component
114-1
114-2
114-3
114-4



















PGF agonist (wt %)
0.01
0.1
1.0
10.0


IC50 the PGF (nM)
1
10
100
1000


Ethanol (wt %)
59.99
59.9
59.4
54.0


Propylene Glycol (wt %)
20.00
20.0
19.8
18.0


Dimethyl Isosorbide (wt %)
20.00
20.0
19.8
18.0









A human male subject suffering from male pattern baldness is treated by a method of this invention. Specifically, for 6 weeks, one of the above compositions is daily administered topically to the subject.
Example 115
A composition for topical administration is made according to the method of Dowton et al., “Influence of Liposomal Composition on Topical Delivery of Encapsulated Cyclosporin A: I. An in vitro Study Using Hairless Mouse Skin”, S.T.P. Pharma Sciences, Vol. 3, pp. 404–407 (1993), using a PGF agonist according to this invention in lieu of cyclosporin A and using the NOVASOME® 1 (available from Micro-Pak, Inc. of Wilmington, Del.) for the non-ionic liposomal formulation.
A human male subject suffering from male pattern baldness is treated each day with the above composition. Specifically, for 6 weeks, the above composition is administered topically to the subject.
Example 116
Shampoos are made, comprising:














Component
Ex. 116-1
Ex. 116-2
Ex. 116-3
Ex. 116-4







Ammonium Lauryl
11.5%  
11.5%  
9.5%  
7.5%  


Sulfate


Ammonium Laureth
4%
3%
2%
2%


Sulfate


Cocamide MEA
2%
2%
2%
2%


Ethylene Glycol
2%
2%
2%
2%


Distearate


Cetyl Alcohol
2%
2%
2%
2%


Stearyl Alcohol
1.2%  
1.2%  
1.2%  
1.2%  


Glycerin
1%
1%
1%
1%


Polyquaternium 10
0.5%  
0.25%  
—
—


Polyquaternium 24
—
—
0.5%  
0.25%  


Sodium Chloride
0.1%  
0.1%  
0.1%  
0.1%  


Sucrose Polyesters of
3%
3%
—
—


Cottonate Fatty Acid


Sucrose Polyesters of
2%
3%
—
—


Behenate Fatty Acid


Polydimethyl Siloxane
—
—
3%
2%


Cocaminopropyl Betaine
—
1%
3%
3%


Lauryl Dimethyl Amine
1.5%  
1.5%  
1.5%  
1.5%  


Oxide


Decyl Polyglucose
—
—
1%
1%


DMDM Hydantoin
0.15%  
0.15%  
0.15%  
0.15%  


PGF agonist having
—
0.2%  
0.2%  
—


IC50 of 200 nM


PGF agonist having
0.1%  
—
—
0.1%  


IC50 of 100 nM


Minoxidil


3%
2%


Phenoxyethanol
0.5%  
0.5%  
0.5%  
0.5%  


Fragrance
0.5%  
0.5%  
0.5%  
0.5%  


Water
q.s.
q.s.
q.s.
q.s.









A human subject suffering from male pattern baldness is treated by a method of this invention. Specifically, for 12 weeks, a shampoo described above is used daily by the subject.
Example 117
Pharmaceutical compositions in liquid form are prepared by conventional methods, formulated as follows:














Ingredient
Quantity




















Prostaglandin Derivative
1
mg



Phosphate buffered physiological saline
10
ml



Methyl Paraben
0.05
ml










An FP antagonist according to this invention is used as the prostaglandin derivative. When 0.25 mL/min of the above composition is instilled into a uterus undergoing premature labor, the above composition decreases uterine contractions within the first hour, preventing premature birth.
Example 118
Pharmaceutical compositions in the form of tablets are prepared by conventional methods, such as mixing and direct compaction, formulated as follows:














Ingredient
Quantity (mg per tablet)



















Prostaglandin Derivative
5



Microcrystalline Cellulose
100



Sodium Starch Glycollate
30



Magnesium Stearate
3










An DP antagonist according to this invention is used as the prostaglandin derivative. When administered orally twice daily, the above composition substantially reduces allergic symptoms in a patient suffering from seasonal allergies.
Example 119
Pharmaceutical compositions in the form of tablets are prepared by conventional methods, such as mixing and direct compaction, formulated as follows:














Ingredient
Quantity (mg per tablet)



















Prostaglandin Derivative
5



Microcrystalline Cellulose
100



Sodium Starch Glycollate
30



Magnesium Stearate
3










An EP1 antagonist according to this invention is used as the prostaglandin derivative. When administered orally four times daily, the above composition substantially reduces pain of headache, migraine pain or muscle pain in a patient.
Example 120
Pharmaceutical compositions in the form of tablets are prepared by conventional methods, such as mixing and direct compaction, formulated as follows:














Ingredient
Quantity (mg per tablet)



















Prostaglandin Derivative
5



Microcrystalline Cellulose
100



Sodium Starch Glycollate
30



Magnesium Stearate
3










An TP antagonist according to this invention is used as the prostaglandin derivative. When administered orally four times daily, the above composition substantially reduces the severity of ulcerative colitis in a patient suffering from that disease.
Example 121
Pharmaceutical compositions in the form of tablets are prepared by conventional methods, such as mixing and direct compaction, formulated as follows:














Ingredient
Quantity (mg per tablet)



















Prostaglandin Derivative
5



Microcrystalline Cellulose
100



Sodium Starch Glycollate
30



Magnesium Stearate
3










An IP agonist according to this invention is used as the prostaglandin derivative. When administered orally twice daily, the above composition substantially reduces blood pressure and improves peripheral circulation in patients suffering from high blood pressure and/or poor peripheral circulation.

## Claims

1. A composition comprising:
(A) a 2-decarboxy-2-phosphinico prostaglandin derivative having a structure selected from the group consisting of:




wherein bond a is selected from the group consisting of a single bond, a trans double bond, and a triple bond;
each of bonds b, c, d, e, and f are independently selected from the group consisting of a single bond and a double bond;
each of bonds g, h, i, and j are independently selected from the group consisting of nil, a single bond, and a double bond;
bond k is selected from the group consisting of a single bond and a double bond;
R1 is selected from the group consisting of a hydrogen atom, a monovalent hydrocarbon group having 1 to 4 carbon atoms, and a monovalent heterogenous group having 1 to 4 member atoms;
R2 is selected from the group consisting of a hydrogen atom, a monovalent hydrocarbon group, a substituted monovalent hydrocarbon group, a monovalent heterogeneous group, a substituted monovalent heterogeneous group, a carbocyclic group, a substituted carbocyclic group, a heterocyclic group, a substituted heterocyclic group, an aromatic group, a substituted aromatic group, a heteroaromatic group, and a substituted heteroaromatic group;
R3 is selected from the group consisting of an oxygen atom, a sulfur atom, and NH;
R4 is selected from the group consisting of an oxygen atom and a sulfur atom;
R5 is a divalent group selected from the group consisting of a hydrocarbon group, a substituted hydrocarbon group, a heterogeneous group, and a substituted heterogeneous group;
R6 is nil or a divalent group selected from the group consisting of —CH2—, —C(O)— and —C(R10)(OR10)—;
R7 is nil or a divalent group having the formula —(CD(D))p-X—(CD(D))q-, wherein p is an integer from 0 to 3 and q is an integer from 0 to 3, X is selected from the group consisting of an oxygen atom, a divalent hydrocarbon group, a sulfur atom, SO, SO2, and ND, and each D is independently selected from the group consisting of a hydrogen atom, a monovalent hydrocarbon group of 1 to 4 carbon atoms, and a monovalent heterogenous group of 1 to 4 member atoms;
R8 is selected from the group consisting of a hydrocarbon group, a substituted hydrocarbon group, a heterogenous group, a substituted heterogenous group, a carbocyclic group, a substituted carbocyclic group, a heterocyclic group, a substituted heterocyclic group, an aromatic group, a substituted aromatic group, a heteroaromatic group, and a substituted heteroaromatic group;
R9 is selected from the group consisting of a hydrogen atom, a monovalent hydrocarbon group of 1 to 4 carbon atoms, and a monovalent heterogenous group of 1 to 4 member atoms;
R10 is selected from the group consisting of a hydrogen atom, a monovalent hydrocarbon group of 1 to 4 carbon atoms, and a monovalent heterogenous group of 1 to 4 member atoms;
M1, M2, M3, and M4 are each independently selected from the group consisting of a carbon atom and a heteroatom, with the proviso that no two heteroatoms may be adjacent;
R11, R12, R13, and R15 are each independently selected from the group consisting of nil, a halogen atom, a heteroatom, and R2, with the provisos that
optionally, R11 and R12, R12 and R13, or R11 and R13 may be bonded together to form a ring structure such as a carbocyclic group, a heterocyclic group, an aromatic group, a heteroaromatic group, a substituted carbocyclic group, a substituted heterocyclic group, a substituted aromatic group, or a substituted heteroaromatic group,
when R11 is OR9, R12 is a hydrogen atom, and M2 is a carbon atom; R13 is not a hydrogen atom, OR9, a monovalent hydrocarbon group of 1 to 4 carbon atoms, a monovalent heterogenous group of 1 to 4 carbon atoms, a substituted monovalent hydrocarbon group of 1 to 4 carbon atoms, or a substituted monovalent heterogenous group of 1 to 4 carbon atoms,
R13 is not N(R9)(OR9) when bond g is a single bond and R13 is not NOR9 when bond g is a double bond, and
R13 is not OR9 when R11 is OR9; M1, M2, M3, and M4 are each carbon atoms, and R12 is a hydrogen atom;

R16 is selected from the group consisting of —CH2—, —NH—, and —NR19—, wherein R19 is selected from the group consisting of hydrocarbon groups, substituted hydrocarbon groups, heterogenous groups, and substituted heterogenous groups; with the proviso that R19 may optionally be bonded together with R8 to form a ring structure selected from the group consisting of heterocyclic groups and substituted heterocyclic groups;
R17 is selected from the group consisting of —SO2—, C(O)—, and —CH2—;
R18 is selected from the group consisting of a sulfur atom and —CH2—; and
an optical isomer of the structure described above, a diastereomer of the structure, an enantiomer of the structure, a pharmaceutically-acceptable salt of the structure, a biohydrolyzable amide of the structure, a biohydrolyzable ester of the structure, and a biohydrolyzable imide of the structure; and
(B) a carrier.

2. The composition of claim 1, wherein the carrier is selected from the group consisting of systemic and topical carriers.

3. The composition of claim 2, wherein the systemic carrier comprises one or more ingredients selected from the group consisting of a) diluents, b) lubricants, c) binders, d) disintegrants, e) colorants, f) flavors, g) sweeteners, h) antioxidants, j) preservatives, k) glidants, m) solvents, n) suspending agents, o) wetting agents, p) surfactants, and combinations thereof.

4. The composition of claim 2, wherein the topical carrier comprises one or more ingredients selected from the group consisting of water, alcohols, aloe vera gel, allantoin, glycerin, vitamin A and E oils, mineral oil, propylene glycol, PPG-2 myristyl propionate, dimethyl isosorbide, q) emollients, r) propellants, s) solvents, t) humectants, u) thickeners, v) powders, w) fragrances, x) pigments, y) sugars, z) cellulose or a derivative thereof, aa) a salt, bb) Edetate disodium, cc) a pH adjusting additive, and combinations thereof.