# Patent

## Title
Amino-functionalized 1,2,4-trioxanes useful as antimalarial agents and process for preparation thereof

## Abstract

The present invention relates to a novel series of antimalarial amino functionalized 1,2,4-trioxane analogues of formula 4




wherein Ar represents aryl groups such as phenyl, 4-biphenyl, 4-chlorophenyl, 4-methoxyphenyl, 4-methylphenyl and R represents phenyl, 4-methoxyphenyl, 4-chlorophenyl, 3,5-dichlorophenyl, 4-acetylaminophenyl, 1-naphthyl, 2-biphenyl, 4-biphenyl, 2-fluorene, 4-fluorophenyl, 2-trifluoromethylphenyl, 3-trifluoromethylphenyl and 4-trifluoromethylphenyl and the like.

## Background
FIELD OF THE INVENTION
The present invention relates to a novel series of antimalarial amino functionalized 1,2,4-trioxane analogues of formula 4




where Ar is an aryl group such as phenyl, 4-biphenyl, 4-chlorophenyl, 4-methoxyphenyl, 4-methylphenyl and R represents phenyl, 4-methoxyphenyl, 4-chlorophenyl, 3,5-dichlorophenyl, 4-acetylaminophenyl, 1-naphthyl, 2-biphenyl, 4-biphenyl, 2-fluorene, 4-fluorophenyl, 2-trifluoromethylphenyl, 3-trifluoromethylphenyl and 4-trifluoromethylphenyl and the like. Several of these compounds show promising antimalarial activity against multidrug-resistant malaria in mice and hold promise as antimalarial agents against multidrug-resistant malaria.

BACKGROUND OF THE INVENTION
Malaria is one of the most deadly diseases affecting third world countries, and claims more than a million lives annually. While various classes of antimalarial agents are available, chloroquine remains the main stay of therapy against malaria. Increasing resistance of Plasmodium falciparum, the most dangerous of the four malaria parasites that infect humans, to common drugs such as chloroquine has heightened concern about malaria. Extensive programs are underway to screen natural products and synthetic derivatives for new agents. Against this background, isolation of artemisinin as the active principle of Chinese herb Artemisia annua has opened new possibilities in malaria chemotherapy.




Semisynthetic derivatives of artemisinin such as arteether, artemether and artesunic acid, are currently the drugs of choice for the treatment of complicated cases of malaria such as cerebral malaria [For reviews on artemisinin and its analogues see; (a) Klayman, D. L. Science 1985, 228, 1049. (b) Bhattacharya, A. K.; Sharma, R. P. Heterocycles 1999, 51, 1681. (c) Borstnik, K.; Paik, I.; Shapiro, T. A.; Posner, G. H. Int. J. Parasitol. 2002, 32, 1661. (d) Ploypradith, P. Acta Trop. 2004, 89, 329. (e) O'Neill, P. M.; Posner, G. H. J. Med. Chem. 2004, 47, 2945]. The limited availability of artemisinin from natural sources and the fact that endoperoxide linkage present in form of a 1,2,4-trioxane ring system is the antimalarial pharmacophore of these compounds, has led to the present effort to develop structurally simple synthetic trioxanes. Several structurally simple synthetic 1,2,4-trioxanes have shown promising antimalarial activity [(a) Bhattacharya, A. K.; Sharma, R. P. Heterocycles 1999, 51, 1681. (b) Borstnik, K.; Paik, L.; Shapiro, T. A.; Posner, G. H. Int. J. Parasitol. 2002, 32, 1661. (c) Singh, C.; Misra, D.; Saxena, G.; Chandra, S. Bioorg. Med. Chem. Lett. 1995, 5, 1913. (d) Singh, C.; Puri, S. K. U.S. Pat. No. 6,316,493 B1, 2001. (e) Singh, C.; Gupta, N.; Puri, S. K. Bioorg. Med. Chem. Lett. 2003, 13, 3445. (f) Singh, C.; Tiwari, P.; Puri, S. K. PCT patent application No. PCT/1N02/00093, dated 28, Mar. 2002]. Singh et al. have developed a novel photooxygenation route for preparation of 1,2,4-trioxanes. β-Hydroxyhydroperoxide prepared by regiospecific photooxygenation of allylic alcohols on condensation with aldehydes or ketone in presence of acid catalyst furnish 1,2,4-trioxane. [(a) Singh, C.; Misra, D.; Saxena, G.; Chandra, S. Bioorg. Med. Chem. Lett. 1992, 2, 497. (b) Singh, C.; Misra, D.; Saxena, G.; Chandra, S. Bioorg. Med. Chem. Lett. 1995, 5, 1913. (c) Singh, C.; Puri, S. K. U.S. Pat. No. 6,316,493 B1, 2001. (d) Singh, C.; Gupta, N.; Puri, S. K. Bioorg. Med. Chem. Lett. 2002, 12, 1913. (e) Singh, C.; Gupta, N.; Puri, S. K. Bioorg. Med. Chem. Lett. 2003, 13, 3445. (f) Singh, C.; Tiwari, P.; Puri, S. K. PCT patent application No. PCT/1N02/00093 dated 28, Mar. 2002]. This method has been extended to prepare several amino functionalized 1,2,4-trioxanes, some of which have shown moderate antimalarial activity against multidrug resistant P. yoelii in mice [Singh, C.; Malik, H.; Puri, S. K. Bioorg. Med. Chem. Lett. 2004, 14, 459]. It is desirable to develop new compounds with show high degrees of anti-malarial activity in order to overcome the problems associated with the prior art compounds including the problem of drug-resistance in the malarial virus.
OBJECTS OF THE INVENTION
The main objective of the present invention is to provide novel amino functionalized 1,2,4-trioxanes with a potential to be used for the treatment of malaria.
It is another object of the invention to provide a process for the preparation of novel amino functionalized 1,2,4-trioxanes of formula 4, a new series of antimalarial agents.
SUMMARY OF THE INVENTION
The present invention provides substituted amino functionalized 1,2,4-trioxanes of formula 4




wherein Ar represents aryl groups selected from the groups consisting of phenyl, 4-biphenyl, 4-chlorophenyl, 4-methoxyphenyl and 4-methylphenyl and R represents phenyl, 4-methoxyphenyl, 4-chlorophenyl, 3,5-dichlorophenyl, 4-acetylaminophenyl, 1-naphthyl, 2-biphenyl, 4-biphenyl, 2-fluorene, 4-fluorophenyl, 2-trifluoromethylphenyl, 3-trifluoromethylphenyl and 4-trifluoromethylphenyl and the like.

In one embodiment of the invention, the compound of formula 4 has structural formula 4aa–4ai shown below:




In another embodiment of the invention, the compound of formula 4 has structural formulae 4ba–4bi shown below:




In another embodiment of the invention, the compound of formula 4 has structural formulae 4ca–4cp shown below:




In another embodiment of the invention, the compound of formula 4 has structural formulae 4da–4dn shown below:




In another embodiment of the invention, the compound of formula 4 has structural formulae 4ea–4eo shown below:




The present invention also provides a method for the preparation of a substituted amino functionalized-1,2,4-trioxanes of formula 4




wherein Ar represents aryl groups selected from the groups consisting of phenyl, 4-biphenyl, 4-chlorophenyl, 4-methoxyphenyl and 4-methylphenyl and R represents phenyl, 4-methoxyphenyl, 4-chlorophenyl, 3,5-dichlorophenyl, 4-acetylaminophenyl, 1-naphthyl, 2-biphenyl, 4-biphenyl, 2-fluorene, 4-fluorophenyl, 2-trifluoromethylphenyl, 3-trifluoromethylphenyl and 4-trifluoromethylphenyl and the like, the process comprising reacting a keto trioxane of formula 3





with an aromatic amine in the presence of NaBH(OAc)3 in CH2Cl2 to furnish amino functionalized 1,2,4-trioxanes of formula 4.

In one embodiment of the invention, the aromatic amine is selected from the group consisting of aniline, 4-methoxyaniline, 4-chloroaniline, 3,5-dichloroaniline, 4-aminoacetanilide, 1-naphthylamine, 2-aminobiphenyl, 4-aminobiphenyl, 2-aminofluorene, 4-fluoroaniline, 2-trifluoromethylaniline, 3-trifluoromethylaniline and 4-trifluoromethylaniline.
In another embodiment of the invention, the compound of formula 4 is obtained by:

    (i) Photooxygenating an allylic alcohol of formula 1 by passing oxygen gas or air in a solution of the alcohol in an organic solvent and in the presence of a dye and a light source which provides visible light, for a period of 4 h, to obtain a β-hydroxyhydroperoxide of formula 2






    (ii) reacting the hydroperoxides of formula 2 in situ with 1,4-cyclohexanedione in the presence of an acid catalyst to give a trioxane of formula 3;






    (iii) reacting the keto trioxane of formula 3 with an aromatic amine in the presence of NaBH(OAc)3 in CH2Cl2 to obtain amino functionalized 1,2,4-trioxane of formula 4.


In one embodiment of the invention, the aromatic amines is selected from the group consisting of aniline, 4-methoxyaniline, 4-chloroaniline, 3,5-dichloroaniline, 4-aminoacetanilide, 1-naphthylamine, 2-aminobiphenyl, 4-aminobiphenyl, 2-aminofluorene, 4-fluoroaniline, 2-trifluoromethylaniline, 3-trifluoromethylaniline and 4-trifluoromethylaniline.
The present invention also provides a method of treating a subject having malaria, comprising administering to the subject a pharmaceutically effective amount of a composition containing the substituted amino functionalized-1,2,4-trioxanes of formula 4, wherein Ar represents aryl groups selected from the groups consisting of phenyl, 4-biphenyl, 4-chlorophenyl, 4-methoxyphenyl and 4-methylphenyl and R represents phenyl, 4-methoxyphenyl, 4-chlorophenyl, 3,5-dichlorophenyl, 4-acetylaminophenyl, 1-naphthyl, 2-biphenyl, 4-biphenyl, 2-fluorene, 4-fluorophenyl, 2-trifluoromethylphenyl, 3-trifluoromethylphenyl and 4-trifluoromethylphenyl and a pharmaceutically acceptable carrier. The composition is administered intramuscularly or orally. The pharmaceutically acceptable amount of compound of formula 4 is in the range of 12 to 96 mg of compound of formula 4 per kilogram of body weight of subject per day.


DETAILED DESCRIPTION OF THE INVENTION
The present invention provides a process for the preparation of amino functionalized 1,2,4-trioxanes of formula 4 given above. Our SAR studies on amino functionalized 1,2,4-trioxanes have resulted in several novel amino functionalized 1,2,4-trioxanes with a high order of antimalarial activity against rodent malaria. The structures and activity of these compounds are disclosed here. The order of activity of amino functionalized 1,2,4-trioxanes reported here is much higher than amino functionalized 1,2,4-trioxanes reported by us earlier [Singh, C.; Malik, H.; Puri, S. K. Bioorg. Med. Chem. Lett. 2004, 14, 459]. The present invention also provides for the preparation of novel amino functionalized 1,2,4-trioxanes of formula 4 with high order of antimalarial activity. The present invention particularly relates to the preparation of amino functionalized 1,2,4-trioxanes of formula 4 wherein Ar represents phenyl, 4-biphenyl, 4-chlorophenyl, 4-methoxyphenyl, 4-methylphenyl and the like and R represents phenyl, 4-methoxyphenyl, 4-chlorophenyl, 3,5-dichlorophenyl, 4-acetylaminophenyl, 1-naphthyl, 2-biphenyl, 4-biphenyl, 2-fluorene, 4-fluorophenyl, 2-trifluoromethylphenyl, 3-trifluoromethylphenyl and 4-trifluoromethylphenyl and the like. These trioxanes are new compounds and are useful as antimalarial agents. These compounds have been tested against multidrug-resistant malaria in mice and have shown high order of antimalarial activity. The trioxanes thus hold promise of use for treatment of malaria, a highly prevalent parasitic disease. The amino functionalized 1,2,4-trioxanes of formula 4 are new chemical entities and have not been prepared earlier.
The process of the invention follows the general scheme shown below:




Scheme 1 Reagents and conditions: (a) hv, O2, methylene blue, MeCN, −10 to 0° C., 4 h. (b) 1,4-cyclohexanedione, concd HCl, 5° C., 18 h. (c) RNH2, NaBH(OAc)3, CH2Cl2, π, 3.5 h.

Allylic alcohol of formula 1 was prepared by known procedure [(a) Singh, C. Tetrahedron Lett. 1990, 31, 6901. (b) Singh, C.; Tiwari, P.; Puri, S. K. PCT application No. PCT/1N02/00093 (28, Mar. 2002)]. Photooxygenation of allylic alcohols of formula 1 is effected by passing oxygen gas or air in the solution of alcohol in an organic solvent and in the presence of a dye and a light source which provides visible light, for a period of 4 h, to furnish β-hydroxyhydroperoxide of formula 2. The hydroperoxides of formula 2 are known compounds and have been prepared by us earlier [(a) Singh, C.; Misra, D.; Saxena, G.; Chandra, S. Bioorg. Med. Chem. Lett. 1992, 2, 497. (b) Singh, C.; Misra, D.; Saxena, G.; Chandra, S. Bioorg. Med. Chem. Lett. 1995, 5, 1913. (c) Singh, C.; Tiwari, P.; Puri, S. K. PCT Application No. PCT/1N02/00093 dated 28, Mar. 2002]. The dye acts as a sensitizer, i.e., converts 3O2 to highly reactive 1O2. Hydroperoxides of formula 2 are reacted in situ with 1,4-cyclohexanedione in the presence of an acid catalyst to give trioxanes of formula 3. These 1,2,4-trioxanes of formula 3 have been tested against malaria parasites in mice and show only moderate order of activity. Keto trioxanes 3a, 3b are known compounds as they have been prepared earlier by us [Singh, C.; Malik, H.; Puri, S. K. Bioorg. Med. Chem. Lett. 2004, 14, 459] while 3c, 3d, 3e are new compounds and have not been prepared earlier. Keto trioxanes of formula 3 are reacted with aromatic amines in the presence of NaBH(OAc)3 in CH2Cl2 to furnish amino functionalized 1,2,4-trioxanes of formula 4. Aromatic amines used may be aniline, 4-methoxyaniline, 4-chloroaniline, 3,5-dichloroaniline, 4-aminoacetanilide, 1-naphthylamine, 2-aminobiphenyl, 4-aminobiphenyl, 2-aminofluorene, 4-fluoroaniline, 2-trifluoromethylaniline, 3-trifluoromethylaniline and 4-trifluoromethylaniline and the like. All the amino functionalized 1,2,4-trioxanes are new chemical entities and have not been prepared earlier. The amino functionalized 1,2,4-trioxanes of formula 4 have been tested against malaria parasites in mice and have shown high order of antimalarial activity.
This invention is further illustrated by the following examples which should not, however, be construed to limit the scope of the present invention.
Example 1
3-(1-Phenyl-Vinyl)-1,2,5-Trioxaspiro[5.5]undec-9-one (Compound 3a, Ar=phenyl)
Solution of allylic alcohol 1a (1 g, 6.75 mmol) and methylene blue (30 mg) in acetonitrile (100 ml) maintained at 0° C., was irradiated with 500 W tungsten-halogen lamp while oxygen was bubbled slowly into reaction mixture for 4 h. 1,4-Cyclohexanedione (1.15 g, 10.13 mmol) and concd. HCl (5 drops) were added and reaction mixture was left at 5° C. overnight.
Reaction mixture was concentrated under reduced pressure and residue taken up in ether (100 ml) was washed with sat. aq NaHCO3 (30 ml). Aqueous layer was extracted with ether (2×75 ml), combined ether layer dried over anhyd. Na2SO4 and concentrated. Crude product was purified by column chromatography on silica gel using EtOAc-hexane (5:95) as eluant to furnish trioxane 3a (0.94 g, 51% yield, based on allylic alcohol 1a used), m.p. 70–71° C.
Example 2
(4-Chloro-Phenyl)-[3-(1-Phenyl-Vinyl)-1,2,5-Trioxa-Spiro[5.5]undec-9-yl]-Amine (Compound 4aa, Formula 4, Ar=Phenyl, R=4-Chlorophenyl)
Acetic acid (1 ml) is added to mixture of trioxane 3a (0.4 g, 1.45 mmol) and 4-chloroaniline (0.23 g, 1.82 mmol) in dichloromethane (20 ml). Reaction mixture was stirred at room temp for half an hour. Sodium triacetoxyborohydride (0.46 g, 3.63 mmol) was added slowly and was stirred for 3 h at room temperature. Reaction mixture was then poured into water and extracted with CH2Cl2 (2×20 ml). Combined organic layer was dried over anhyd. Na2SO4, concentrated under vacuum. Crude product was chromatographed over silica gel using benzene-hexane (50:50) as eluant to furnish amino functionalized 1,2,4-trioxane 4aa as an inseparable mixture of diastereomers as oil (0.40 g, 71.7% yield).
Example 3
Biphenyl-2-yl-[3-(1-Phenyl-Vinyl)-1,2,5-Trioxa-Spiro[5.5]undec-9-yl]Amine (Compound 4ab and 4ac, Formula 4, Ar=Phenyl, R=2-Biphenyl)
To mixture of trioxane 3a (0.50 g, 1.82 mmol) and 2-aminobiphenyl (0.38 g, 2.27 mmol) in dichloromethane (20 ml) acetic acid was added (1 ml) and reaction mixture was stirred at room temp for half an hour. Sodium triacetoxyborohydride (0.58 g, 2.73 mmol) was added slowly and it was stirred for 3 h at room temperature. The reaction mixture was then poured into water and extracted with CH2Cl2 (2×20 ml). Combined organic layer was dried over anhyd. Na2SO4, concentrated under vacuum and crude product was chromatographed over silica gel using benzene-hexane (50:50) as eluant to furnish amino functionalized 1,2,4-trioxane 4ab as oil (upper Rf, 0.17 g, 21.8% yield) and 4ac (lower Rf, 0.25 g, 32.6% yield), m.p. 74–76° C.
Example 4
Biphenyl-4-yl-[3-(1-Phenyl-Vinyl)-1,2,5-Trioxa-Spiro[5.5]undec-9-yl]Amine (Compound 4ad, Formula 4, Ar=Phenyl, R=4-Biphenyl)
To a mixture of trioxane 3a (0.40 g, 1.45 mmol) and 4-aminobiphenyl (0.30 g, 1.82 mmol) in dichloromethane (20 ml) acetic acid (1 ml) was added and reaction mixture was stirred at room temp for half an hour. Sodium triacetoxyborohydride (0.46 g, 3.63 mmol) was added slowly and it was stirred for 3 h at room temperature. Reaction mixture was then poured into water and extracted with CH2Cl2 (2×20 ml). Combined organic layer was dried over anhyd. Na2SO4, concentrated under vacuum and crude product was chromatographed over silica gel using benzene-hexane (50:50) as eluant to furnish amino functionalized 1,2,4-trioxane 4ad as an inseparable mixture of diastereomers (0.44 g, 70.5% yield), m.p. 118–120° C.
Example 5
(9H-Fluoren-3-yl)-[1-Phenyl-Vinyl)-1,2,5-Trioxa-Spiro[5.5]undec-9-yl]-Amine (Compound 4ae, Formula 4, Ar=Phenyl, R=2-Fluorene)
To a mixture of trioxane 3a (0.30 g, 1.09 mmol) and 2-aminofluorene (0.24 g, 1.36 mmol) in dichloromethane (20 ml) was added acetic acid (1 ml) and reaction mixture was stirred at room temp for half an hour. Sodium triacetoxyborohydride (0.35 g, 1.64 mmol) was added slowly and it was stirred for 3 h at room temperature. The reaction mixture was then poured into water and extracted with CH2Cl2 (2×15 ml). Combined organic layer was dried over anhyd. Na2SO4, concentrated under vacuum and the crude product was chromatographed over silica gel using benzene-hexane (50:50) as eluant to furnish amino functionalized 1,2,4-trioxane 4ae as an inseparable mixture of diastereomers (0.30 g, 63.5% yield), m.p. 144–146° C.
Example 6
(4-Fluoro-Phenyl)-[3-(1-Phenyl-Vinyl)-1,2,5-Trioxa-Spiro[5.5]undec-9-yl]-Amine (Compound 4af, Formula 4, Ar=Phenyl, R=4-Fluorophenyl)
To a mixture of trioxane 3a (0.30 g, 1.09 mmol) and 4-fluoroaniline (0.15 g, 1.34 mmol) in dichloromethane (20 ml) was added acetic acid (1 ml) and reaction mixture was stirred at room temp for half an hour. Sodium triacetoxyborohydride (0.35 g, 1.65 mmol) was added slowly and it was stirred for 3 h at room temperature. The reaction mixture was then poured into water and extracted with CH2Cl2 (2×20 ml). Combined organic layer was dried over anhyd. Na2SO4, concentrated under vacuum and the crude product was chromatographed over silica gel using benzene-hexane (50:50) as eluant to furnish amino functionalized 1,2,4-trioxane 4af as an inseparable mixture of diastereomers as oil (0.34 g, 84.1% yield).
Example 7
[3-(1-Phenyl-Vinyl)-1,2,5-Trioxa-Spiro[5.5]undec-9-yl]-(3-Trifluoromethyl-Phenyl)-Amine (Compound 4ah, Formula 4, Ar=Phenyl, R=3-Trifluoromethylphenyl)
To a mixture of trioxane 3a (0.50 g, 1.82 mmol) and 3-trifluoromethylaniline (0.37 g, 2.27 mmol) in dichloromethane (20 ml) was added acetic acid (1 ml) and reaction mixture was stirred at room temp for half an hour. Sodium triacetoxyborohydride (0.60 g, 2.73 mmol) was added slowly and it was stirred for 3 h at room temperature. The reaction mixture was then poured into water and extracted with CH2Cl2 (2×20 ml). Combined organic layer was dried over anhyd. Na2SO4, concentrated under vacuum and the crude product was chromatographed over silica gel using benzene-hexane (50:50) as eluant to furnish amino functionalized 1,2,4-trioxane 4ah as an inseparable mixture of diastereomers as oil (0.49 g, 64.1% yield).
Example 8
[3-(1-Phenyl-Vinyl)-1,2,5-Trioxa-Spiro[5.5]undec-9-yl]-(4-Trifluoromethyl-Phenyl-Amine (Compound 4ai, Formula 4, Ar=Phenyl, R=4-Trifluoromethylphenyl)
To a mixture of trioxane 3a (0.30 g, 1.09 mmol) and 4-trifluoromethylaniline (0.22, 1.36 mmol) dichloromethane (20 ml) was added acetic acid (1 ml) and reaction mixture was stirred at room temp for half an hour. Sodium triacetoxyborohydride (0.34 g, 1.60 mmol) was added slowly and it was stirred for 3 h at room temperature. The reaction mixture was then poured into water and extracted with CH2Cl2 (2×20 ml). Combined organic layer was dried over anhyd. Na2SO4, concentrated under vacuum and the crude product was chromatographed over silica gel using benzene-hexane (50:50) as eluant to furnish amino functionalized 1,2,4-trioxane 4ai as an inseparable mixture of diastereomers as oil (0.26 g, 58.7% yield).
Example 9
3-(1-Biphenyl-4yl-Vinyl)-1,2,5-Trioxaspiro[5.5]undec-9-one (Compound 3b, Ar=Biphenyl)
Solution of allylic alcohol 1 h (1 g, 6.75 mmol) and methylene blue (30 mg) in acetonitrile (100 ml) maintained at 0° C., was irradiated with 500 W tungsten-halogen lamp while oxygen was bubbled slowly into reaction mixture for 4 h. 1,4-Cyclohexanedione (1.15 g, 10.13 mmol) and concd HCl (5 drops) were added and the reaction mixture was left at 5° C. overnight. Reaction mixture was concentrated under reduced pressure and residue taken up in either (100 ml) was washed with sat. aq NaHCO3 (30 ml). The aqueous layer was extracted with ether (2×75 ml), combined ether layer dried over anhyd. Na2SO4 and concentrated. The crude product was purified by column chromatography on silica gel using EtOAc-hexane (5:95) as eluant to furnish trioxane 3b (0.65 g, 42% yield, based on allylic alcohol 1b used), m.p. 104–105° C.
Example 10
[3-(1-Biphenyl-4-yl-Vinyl)-1,2,5-Trioxa-Spiro[5.5]undec-9-yl]-(4-Chloro-Phenyl)-Amine (Compound 4ba, Formula 4, Ar=4-Biphenyl, R=4-Chlorophenyl)
To a mixture of trioxane 3b (0.40 g, 1.14 mmol) and 4-chloroaniline (0.18 g, 1.42 mmol) in dichloromethane (20 ml) was added acetic acid (1 ml) and reaction mixture was stirred at room temp for half an hour. Sodium triacetoxyborohydride (0.36 g, 1.71 mmol) was added slowly and it was stirred for 3 h at room temperature. The reaction mixture was then poured into water and extracted with CH2Cl2 (2×15 ml). Combined organic layer was dried over anhyd. Na2SO4, concentrated under vacuum and the crude product was chromatographed over silica gel using benzene-hexane (50:50) as eluant to furnish amino functionalized 1,2,4-trioxane 4ba as an inseparable mixture of diastereomers (0.38 g, 72.1% yield), m.p. 148–150° C.
Example 11
Biphenyl-2-yl-[3-(1-Biphenyl-4-yl-Vinyl)-1,2,5-Trioxaspiro[5.5]undec-9-yl]-Amine (Compound 4bb and 4bc, Formula 4, Ar=4-Biphenyl, R=2-Biphenyl)
Acetic acid (1 ml) is added to mixture of trioxane 3b (0.60 g, 1.71 mmol) and 2-aminobiphenyl (0.36 g, 2.14 mmol) in dichloromethane (25 ml). Reaction mixture was stirred at room temp for half an hour. Sodium triacetoxyborohydride (0.54 g, 2.57 mmol) was added slowly and it was stirred for 3 h at room temperature. Reaction mixture was then poured into water and extracted with CH2Cl2 (2×25 ml). Combined organic layer was dried over anhyd. Na2SO4, concentrated under vacuum. Crude product was chromatographed over silica gel using eluant benzene-hexane (50:50) to give amino functionalized 1,2,4-trioxane 4bb (upper Rf, 0.19 g, 21.7% yield), m.p. 105–106° C. and 4bc (lower Rf, 0.28 g, 32.4% yield), mp 148–150° C.
Example 12
Biphenyl-4-yl-[3-(1-Biphenyl-4-yl-Vinyl)-1,2,5-trioxaspiro[5.5]undec-9-yl]-Amide (Compound 4bd, Formula 4, Ar=4-Biphenyl, R=4-Biphenyl)
To mixture of trioxane 3b (0.50 g, 1.42 mmol) and 4-aminobiphenyl (0.30 g, 1.78 mmol) in dichloromethane (20 ml) was added acetic acid (1 ml) and reaction mixture was stirred at room temp for half an hour. Sodium triacetoxyborohydride (0.45 g, 2.14 mmol) was added slowly and it was stirred for 3 h at room temperature. The reaction mixture was then poured into water and extracted with CH2Cl2 (2×20 ml). Combined organic layer was dried over anhyd. Na2SO4, concentrated under vacuum and the crude product was chromatographed over silica gel using benzene-hexane (50:50) as eluant to furnish amino functionalized 1,2,4-trioxane 4bd as an inseparable mixture of diastereomers (0.53 g, 73.8% yield), m.p. 158–160° C.
Example 13
[3-(1-Biphenyl-4-yl-Vinyl)-1,2,5-Trioxa-Spiro[5.5]undec-9-yl]-(9H-Fluoren-3-yl)-Amine (Compound 4be, Formula 4, Ar=4-Biphenyl, R=2-Fluorene)
To a mixture of trioxane 3b (0.30 g, 0.85 mmol) and 2-aminofluorene (0.19 g, 1.07 mmol) in dichloromethane (20 ml) was added acetic acid (1 ml) and reaction mixture was stirred at room temp for half an hour. Sodium triacetoxyborohydride (0.27 g, 1.64 mmol) was added slowly and it was stirred for 3 h at room temperature. The reaction mixture was then poured into water and extracted with CH2Cl2 (2×15 ml). Combined organic layer was dried over anhyd. Na2SO4, concentrated under vacuum and the crude product was chromatographed over silica gel using benzene-hexane (50:50) as eluant to furnish amino functionalized 1,2,4-trioxane 4be as an inseparable mixture of diastereomers (0.28 g, 65.04% yield), m.p. 170–172° C.
Example 14
3-[1-(4-Chloro-Phenyl)-Vinyl]-1,2,5-Trioxa-Spiro[5.5]undecan-9-one (Compound 3c, Ar=4-Chlorophenyl)
Solution of allylic alcohol 1c (1 g, 5.47 mmol) and methylene blue (30 mg) in acetonitrile (100 ml) maintained at 0° C., was irradiated with 500 W tungsten-halogen lamp while oxygen was bubbled slowly into reaction mixture for 4 h. 1,4-Cyclohexanedione (1.22 g, 10.95 mmol) and concd HCl (5 drops) were added and the reaction mixture was left at 5° C. overnight. Reaction mixture was concentrated under reduced pressure and residue taken up in ether (100 ml) was washed with sat. aq NaHCO3 (30 ml). The aqueous layer was extracted with ether (2×75 ml), combined ether layer dried over anhyd. Na2SO4 and concentrated. The crude product was purified by column chromatography on silica gel using EtOAc-hexane (5:95) as eluant to furnish trioxane 3c (0.64 g, 38% yield, based on allylic alcohol 1c used), m.p. 72–74° C.
Example 15
{3-[1-(4-Chloro-Phenyl)-Vinyl]-1,2,5-Trioxa-Spiro[5.5]undec-9-yl}-Phenyl-Amine (Compound 4ca, Formula 4, Ar=4-Chlorophenyl, R=Phenyl)
To mixture of trioxane 3c (0.50 g, 1.62 mmol) and aniline (0.19 g, 2.02 mmol) in dichloromethane (20 ml) was added acetic acid (1 ml) and reaction mixture was stirred at room temp for half an hour. Sodium triacetoxyborohydride (0.51 g, 2.43 mmol) was added slowly and it was stirred for 3 h at room temperature. The reaction mixture was then poured into water and extracted with CH2Cl2 (2×20 ml). Combined organic layer was dried over anhyd. Na2SO4, concentrated under vacuum and the crude product was chromatographed over silica gel using benzene-hexane (50:50) as eluant to furnish amino functionalized 1,2,4-trioxane 4ca as an inseparable mixture of diastereomers as oil (0.45 g, 73.4% yield)
Example 16
{3-[1-(4-Chloro-Phenyl)-Vinyl]-1,2,5-Trioxa-Spiro[5.5]undec-9-yl}-p-Tolyl-Amine (Compound 4cd, Formula 4, Ar=4-Chlorophenyl, R=4-Methoxyphenyl)
To mixture of trioxane 3c (0.50 g, 2.27 mmol) and 4-methoxyaniline (0.25 g, 2.02 mmol) in dichloromethane (20 ml) was added acetic acid (1 ml) and reaction mixture was stirred at room temp for half an hour. Sodium triacetoxyborohydride (0.51 g, 2.43 mmol) was added slowly and it was stirred for 3 h at room temperature. The reaction mixture was then poured into water and extracted with CH2Cl2 (2×20 ml). Combined organic layer was dried over anhyd. Na2SO4, concentrated under vacuum and the crude product was chromatographed over silica gel using benzene-hexane (50:50) as eluant to furnish amino functionalized 1,2,4-trioxane as an inseparable mixture of diastereomers 4cd (0.54 g, 80.2% yield), m.p. 148–150° C.
Example 17
Biphenyl-2-yl-{3-[1-(4-Chloro-Phenyl)-Vinyl]-1,2,5-Trioxa-Spiro[5.5]undec-9-yl}-Amine (Compound 4ci and 4cj, Formula 4, Ar=4-Chlorophenyl, R=2-Biphenyl)
To a mixture of trioxane 3c (0.50 g, 1.62 mmol) and 2-aminobiphenyl (0.34 g, 2.02 mmol) in dichloromethane (20 ml) was added acetic acid (1 ml) and reaction mixture was stirred at room temp for half an hour. Sodium triacetoxyborohydride (0.51 g, 2.43 mmol) was added slowly and it was stirred for 3 h at room temperature. The reaction mixture was then poured into water and extracted with CH2Cl2 (2×20 ml). Combined organic layer was dried over anhyd. Na2SO4, concentrated under vacuum and the crude product was chromatographed over silica gel using benzene-hexane (50:50) as eluant to furnish amino functionalized 1,2,4-trioxane 4ci (upper Rf, 0.21 g, 28.8% yield) and 4cj (lower Rf, 0.32 g, 43.2% yield) as oil.
Example 18
Biphenyl-4-yl-{3-[1-(4-Chloro-Phenyl)-Vinyl]-1,2,5-Trioxa-Spiro[5.5]undec-9-yl}-Amine (Compound 4ck, Formula 4, Ar=4-Chlorophenyl, R=4-Biphenyl)
To a mixture of trioxane 3c (0.50 g, 1.62 mmol) and 4-aminobiphenyl (0.34 g, 2.02 mmol) in dichloromethane (20 ml) was added acetic acid (1 ml) and reaction mixture was stirred at room temp for half an hour. Sodium triacetoxyborohydride (0.51 g, 2.43 mmol) was added slowly and it was stirred for 3 h at room temperature. The reaction mixture was then poured into water and extracted with CH2Cl2 (2×20 ml). Combined organic layer was dried over anhyd. Na2SO4, concentrated under vacuum and the crude product was chromatographed over silica gel using benzene-hexane (50:50) as eluant to furnish amino functionalized 1,2,4-trioxane 4ck as an inseparable mixture of diastereomers as oil (0.37 g, 49.4% yield).
Example 19
{3-[1-(4-Chloro-Phenyl)-Vinyl]-1,2,5-Trioxa-Spiro[5.5]undec-9-yl}-(9H-Fluoren-3yl)-Amine (Compound 4cl, Formula 4, Ar=4-Chlorophenyl, R=2-Fluorene)
To a mixture of trioxane 3c (0.30 g, 0.97 mmol) and 2-aminofluorene (0.22 g, 1.21 mmol) in dichloromethane (20 ml) was added acetic acid (1 ml) and reaction mixture was stirred at room temp for half an hour. Sodium triacetoxyborohydride (0.30 g, 1.46 mmol) was added slowly and it was stirred for 3 h at room temperature. The reaction mixture was then poured into water and extracted with CH2Cl2 (2×15 ml). Combined organic layer was dried over anhyd. Na2SO4, concentrated under vacuum and the crude product was chromatographed over silica gel using benzene-hexane (50:50) as eluant to furnish amino functionalized 1,2,4-trioxane 4cl as an inseparable mixture of diastereomers (0.19 g, 42.3% yield), m.p. 162–164° C.
Example 20
{3-[1-(4-Chloro-Phenyl)-Vinyl]-1,2,5-Trioxa-Spiro[5.5]undec-9-yl}-(4-Trifluoromethyl-Phenyl)-Amine (Compound 4cp, Formula 4, Ar=4-Chlorophenyl, R=4-Trifluoromethylphenyl)
To mixture of trioxane 3c (0.30 g, 0.97 mmol) and 4-trifluoromethylaniline (0.19 g, 1.21 mmol) in dichloromethane (20 ml) was added acetic acid (1 ml) and reaction mixture was stirred at room temp for half an hour. Sodium triacetoxyborohydride (0.30 g, 1.46 mmol) was added slowly and it was stirred for 3 h at room temperature. Reaction mixture was then poured into water and extracted with CH2Cl2 (2×15 ml). Combined organic layer was dried over anhyd. Na2SO4, concentrated under vacuum and crude product was chromatographed over silica gel using benzene-hexane (50:50) as eluant to furnish amino functionalized 1,2,4-trioxane 4cp as an inseparable mixture of diastereomers as oil (0.21 g, 47.6% yield).
Example 21
3-[1-(4-Methoxy-Phenyl)-Vinyl]-1,2,5-Trioxa-Spiro[5.5]undecan-9-one (Compound 3d, Ar=4-Methoxyphenyl)
Solution of allylic alcohol 1d (1 g, 5.61 mmol) and methylene blue (30 mg) in acetonitrile (100 ml) maintained at 0° C., was irradiated with a 500 W tungsten-halogen lamp while oxygen was bubbled slowly into the reaction mixture for 4 h. 1,4-Cyclohexanedione (1.25 g, 11.22 mmol) and concd HCl (5 drops) were added and the reaction mixture was left at 5° C. overnight. Reaction mixture was concentrated under reduced pressure and residue taken up in ether (100 ml) was washed with sat. aq NaHCO3 (30 ml). Aqueous layer was extracted with ether (2×75 ml), combined ether layer dried over anhyd. Na2SO4 and concentrated. Crude product was purified by column chromatography on silica gel using EtOAc-hexane (5:95) as eluant to furnish trioxane 3d as oil (0.48 g, 28.1% yield, based on allylic alcohol 1d used).
Example 22
{3-[1-(4-Methoxy-Phenyl)-Vinyl]-1,2,5-Trioxa-Spiro[5.5]undec-9-yl}-Phenyl-Amine (Compound 4da, Formula 4, Ar=4-Methoxyphenyl, R=Phenyl)
To a mixture of trioxane 3d (0.50 g, 1.62 mmol) and aniline (0.19 g, 2.05 mmol) in dichloromethane (20 ml) was added acetic acid (1 ml) and reaction mixture was stirred at room temp for half an hour. Sodium triacetoxyborohydride (0.52 g, 2.46 mmol) was added slowly and it was stirred for 3 h at room temperature. The reaction mixture was then poured into water and extracted with CH2Cl2 (2×20 ml). Combined organic layer was dried over anhyd. Na2SO4, concentrated under vacuum and the crude product was chromatographed over silica gel using benzene-hexane (50:50) as eluant to furnish amino functionalized 1,2,4-trioxane 4da as an inseparable mixture of diastereomers as oil (0.47 g, 74.9% yield).
Example 23
(4-Methoxy-Phenyl)-{3-[1-(4-Methoxy-Phenyl)-Vinyl]-1,2,5-Trioxa-Spiro [5.5]undec-9-yl}-Amine (Compound 4db, Formula 4, Ar=4-Methoxyphenyl, R=4-Methoxyphenyl)
To a mixture of trioxane 3d (0.50 g, 1.54 mmol) and 4-methoxyaniline (0.25 g, 2.05 mmol) in dichloromethane (20 ml) was added acetic acid (1 ml) and reaction mixture was stirred at room temp for half an hour. Sodium triacetoxyborohydride (0.52 g, 2.46 mmol) added slowly and it was stirred for 3 h at room temperature. The reaction mixture was then poured into water and extracted with CH2Cl2 (2×20 ml). Combined organic layer was dried over anhyd. Na2SO4, concentrated under vacuum and the crude product was chromatographed over silica gel using benzene-hexane (50:50) as eluant to furnish amino functionalized 1,2,4-trioxane 4db as an inseparable mixture of diastereomers (0.53 g, 79.2% yield), m.p. 126–128° C.
Example 24
(4-Chloro-Phenyl)-{3-[1-(4-Methoxy-Phenyl)-Vinyl]-1,2,5-Trioxa-Spiro[5.5]undec-9-yl}-Amine (Compound 4dc, Formula 4, Ar=4-Methoxyphenyl, R=4-Chlorophenyl)
To a mixture of trioxane 3d (0.50 g, 1.62 mmol) and 4-chloroaniline (0.19 g, 2.05 mmol) in dichloromethane (20 ml) was added acetic acid (1 ml) and reaction mixture was stirred at room temp for half an hour. Sodium triacetoxyborohydride (0.52 g, 2.46 mmol) was added slowly and it was stirred for 3 h at room temperature. The reaction mixture was then poured into water and extracted with CH2Cl2 (2×20 ml). Combined organic layer was dried over anhyd. Na2SO4, concentrated under vacuum and the crude product was chromatographed over silica gel using benzene-hexane (50:50) as eluant to furnish amino functionalized 1,2,4-trioxane 4dc as an inseparable mixture of diastereomers (0.47 g, 74.9% yield), m.p. 104–106° C.
Example 25
(3,5-Dichloro-Phenyl)-{3-[1-(4-Methoxy-Phenyl)-Vinyl]-1,2,5-Trioxa-Spiro[5.5]undec-9-yl}-Amine (Compound 4dd, Formula 4, Ar=4-Methoxyphenyl, R=3,5-Dichlorophenyl)
To mixture of trioxane 3d (0.50 g, 1.64 mmol) and 3,5-dichloroaniline (0.33 g, 2.05 mmol) in dichloromethane (20 ml) was added acetic acid (1 ml) and reaction mixture was stirred at room temp for half an hour. Sodium triacetoxyborohydride (0.52 g, 2.46 mmol) was added slowly and it was stirred for 3 h at room temperature. The reaction mixture was then poured into water and extracted with CH2Cl2 (2×20 ml). Combined organic layer was dried over anhyd. Na2SO4, concentrated under vacuum and the crude product was chromatographed over silica gel using benzene-hexane (50:50) as eluant to furnish amino functionalized 1,2,4-trioxane 4dd as an inseparable mixture of diastereomers (0.38 g, 51.3% yield), m.p. 85–86° C.
Example 26
N-(4-{3-[1-(4-Methoxy-Phenyl)-Vinyl]-1,2,5-Trioxa-Spiro[5.5]undec-9-ylamino}-Phenyl)-Acetamide (Compound 4de, Formula 4, Ar=4-Methoxyphenyl, R=4-Acetylaminophenyl)
To a mixture of trioxane 3d (0.50 g, 1.64 mmol) and 4-aminoacetanilide (0.30 g, 2.05 mmol) in dichloromethane (20 ml) was added acetic acid (1 ml) and reaction mixture was stirred at room temp for half an hour. Sodium triacetoxyborohydride (0.52 g, 2.46 mmol) was added slowly and it was stirred for 3 h at room temperature. The reaction mixture was then poured into water and extracted with CH2Cl2 (2×20 ml). Combined organic layer was dried over anhyd. Na2SO4, concentrated under vacuum and the crude product was chromatographed over silica gel using EtOAc-hexane (50:50) as eluant to furnish amino functionalized 1,2,4-trioxane 4de as an inseparable mixture of diastereomers (0.60 g, 83.3% yield), m.p. 70–72° C.
Example 27
{3-[1-(4-Methoxy-Phenyl)-Vinyl]-1,2,5-Trioxa-Spiro[5.5]undec-9-yl}-(3-Trifluoromethyl-Phenyl)-Amine (Compound 4dm, Formula 4, Ar=4-Methoxyphenyl, R=3-Trifluoromethylphenyl)
To mixture of trioxane 3d (0.30 g, 0.98 mmol) and 3-trifluoromethylaniline (0.20 g, 1.21 mmol) in dichloromethane (20 ml) was added acetic acid (1 ml) and reaction mixture was stirred at room temp for half an hour. Sodium triacetoxyborohydride (0.31 g, 1.47 mmol) was added slowly and it was stirred for 3 h at room temperature. Reaction mixture was then poured into water and extracted with CH2Cl2 (2×15 ml). Combined organic layer was dried over anhyd. Na2SO4, concentrated under vacuum and crude product was chromatographed over silica gel using EtOAc-hexane (50:50) as eluant to furnish amino functionalized 1,2,4-trioxane 4dm as an inseparable mixture of diastereomers as oil (0.27 g, 60.9% yield).
Example 28
3-(1-p-Tolyl-Vinyl)-1,2,5-Trioxa-Spiro[5.5]undecan-9-one (Compound 3e, Ar=4-Methylphenyl)
Solution of allylic alcohol 1e (1 g, 6.16 mmol) and methylene blue (30 mg) in acetonitrile (100 ml) maintained at 0° C. was irradiated with a 500 W tungsten-halogen lamp while oxygen was bubbled slowly into reaction mixture for 4 h. 1,4-Cyclohexanedione (1.15 g, 10.13 mmol) and concd HCl (5 drops) were added and reaction mixture was left at 5° C. overnight. Reaction mixture was concentrated under reduced pressure and residue taken up in ether (100 ml) was washed with sat. aq NaHCO3 (30 ml). The aqueous layer was extracted with ether (2×75 ml), combined ether layer dried over anhyd. Na2SO4 and concentrated. The crude product was purified by column chromatography on silica gel using EtOAc-hexane (5:95) as eluant to furnish trioxane 3e (0.58 g, 32.7% yield, based on allylic alcohol 1e used), m.p. 66–68° C.
Example 29
Phenyl-[3-(1-p-Tolyl-Vinyl)-1,2,5-Trioxa-Spiro[5.5]undec-9-yl]-Amine (Compound 4ea, Formula 4, Ar=4-Methylphenyl, R=Phenyl)
To a mixture of trioxane 3e (0.30 g, 1.04 mmol) and aniline (0.12 g, 1.30 mmol) in dichloromethane (20 ml) was added acetic acid (1 ml) and reaction mixture was stirred at room temp for half an hour. Sodium triacetoxyborohydride (0.33 g, 1.56 mmol) was added slowly and it was stirred for 3 h at room temperature. The reaction mixture was then poured into water and extracted with CH2Cl2 (2×20 ml). Combined organic layer was dried over anhyd. Na2SO4, concentrated under vacuum and the crude product was chromatographed over silica gel using benzene-hexane (50:50) as eluant to furnish amino functionalized 1,2,4-trioxane 4ea as an inseparable mixture of diastereomers as oil (0.29 g, 76.3% yield).
Example 30
(4-Methoxy-Phenyl)-[3-(1-p-Tolyl-Vinyl)-1,2,5-Trioxa-Spiro[5.5]undec-9-yl]-Amine (Compound 4eb, Formula 4, Ar=4-Methylphenyl, R=4-Methoxyphenyl)
To a mixture of trioxane 3e (0.30 g, 1.04 mmol) and 4-methoxyaniline (0.16 g, 1.30 mmol) in dichloromethane (20 ml) was added acetic acid (1 ml) and reaction mixture was stirred at room temp for half an hour. Sodium triacetoxyborohydride (0.33 g, 1.56 mmol) was added slowly and it was stirred for 3 h at room temperature. The reaction mixture was then poured into water and extracted with CH2Cl2 (2×15 ml). Combined organic layer was dried over anhyd. Na2SO4, concentrated under vacuum and the crude product was chromatographed over silica gel using benzene-hexane (50:50) as eluant to furnish amino functionalized 1,2,4-trioxane 4eb as an inseparable mixture of diastereomers as oil (0.33 g, 80.2% yield).
Example 31
(4-Chloro-Phenyl)-[3-(1-p-Tolyl-Vinyl)-1,2,5-Trioxa-Spiro[5.5]undec-9-yl]-Amine (Compound 4ec Formula 4, Ar=4-Methylphenyl, R=4-Chlorophenyl)
Acetic acid (1 ml) is added to mixture of trioxane 3e (0.30 g, 1.04 mmol) and 4-chloroaniline (0.16 g, 1.30 mmol) in dichloromethane (20 ml). Reaction mixture was stirred at room temp for half an hour. Sodium triacetoxyborohydride (0.33 g, 1.56 mmol) was added slowly and it was stirred for 3 h at room temperature. Reaction mixture was then poured into water and extracted with CH2Cl2 (2×15 ml). Combined organic layer was dried over anhyd. Na2SO4, concentrated under vacuum. Crude product was chromatographed over silica gel using benzene-hexane (50:50) as eluant to furnish amino functionalized 1,2,4-trioxane 4ec as an inseparable mixture of diastereomers (0.26 g, 62.6% yield), m.p. 110–112° C.
Example 32
(3,5-Dichloro-Phenyl)-[3-(1-Tolyl-Vinyl)-1,2,5-Trioxa-Spiro[5.5]undec-9-yl]-Amine (Compound 4ed (upper Rf) and 4ee (lower Rf), Formula 4, Ar=4-Methylphenyl, R=3,5-Dichlorophenyl)
Acetic acid (1 ml) is added to mixture of trioxane 3e (0.50 g, 1.62 mmol) and 3,5-dichloroanile (0.19 g, 2.05 mmol) in dichloromethane (20 ml) and reaction mixture stirred at room temp for half an hour. Sodium triacetoxyborohydride (0.52 g, 2.46 mmol) was added slowly and stirred for 3 h at room temperature. Reaction mixture was poured into water and extracted with CH2Cl2 (2×20 ml). Combined organic layer was dried over anhyd. Na2SO4, concentrated under vacuum. Crude product chromatographed over silica gel using benzene-hexane (50:50) as eluant to furnish amino functionalized 1,2,4-trioxane 4ed (upper Rf, 0.19 g, 25.20% yield), m.p. 140–142° C. and 4ee (lower Rf, 0.28 g, 37.1% yield), m.p. 112–114° C.
Example 33
N-{4-[3-(1-p-Tolyl-Vinyl)-1,2,5-Trioxa-Spiro[5.5]undec-9-ylamino]-Phenyl}-Acetamide (Compound 4ef, Formula 4, Ar=4-Methylphenyl, R=4-Acetylaminophenyl)
Acetic acid (1 ml) was added to mixture of trioxane 3e (0.50 g, 1.62 mmol) and 4-aminoacetanilide (0.19 g, 2.05 mmol) in dichloromethane (20 ml). Reaction mixture was stirred at room temp for half an hour. Sodium triacetoxyborohydride (0.52 g, 2.46 mmol) was added slowly and stirred for 3 h at room temperature. Reaction mixture was then poured into water and extracted with CH2Cl2 (2×20 ml). Combined organic layer was dried over anhyd. Na2SO4, and concentrated under vacuum. Crude product was chromatographed over silica gel using EtOAc-hexane (50:50) as eluant to furnish amino functionalized 1,2,4-trioxane 4ef as an inseparable mixture of diastereomers (0.47 g, 76.2% yield), m.p. 83–85° C.
Example 34
Naphthalen-1-yl-[3-(1-p-Tolyl-Vinyl)-1,2,5-Trioxa-Spiro[5.5]undec-9-yl]-Amine (Compound 4eg, Formula 4, Ar=4-Methylphenyl, R=1-Naphthyl)
Acetic acid (1 ml) was added to mixture of trioxane 3e (0.50 g, 1.64 mmol) and 1-naphthylamine (0.30 g, 2.05 mmol) in dichloromethane (20 ml). Reaction mixture was stirred at room temperature for half an hour. Sodium triacetoxyborohydride (0.52 g, 2.46 mmol) was added slowly and stirred for 3 h at room temperature. Reaction mixture was then poured into water and extracted with CH2Cl2 (2×20 ml). Combined organic layer was dried over anhyd. NaSO4, and concentrated under vacuum. Crude product was chromatographed over silica gel using benzene-hexane (50:50) as eluant to furnish amino functionalized 1,2,4-trioxane 4eg as an inseparable mixture of diastereomers (0.52 g, 72.2% yield), m.p. 70–72° C.
Example 35
Biphenyl-2-yl-[3-(1-p-Tolyl-Vinyl)-1,2,5-Trioxa-Spiro[5.5]undec-9-yl]-Amine (Compound 4eh and 4ei, Formula 4, Ar=4-Methylphenyl, R=2-Biphenyl)
Acetic acid (1 ml) was added to mixture of trioxane 3e (0.80 g, 2.59 mmol) and 2-aminobiphenyl (0.41 g, 3.24 mmol) in dichloromethane (30 ml). Reaction mixture was stirred at room temp for half an hour. Sodium triacetoxyborohydride (0.82 g, 3.89 mmol) was added slowly and it was stirred for 3 h at room temperature. The reaction mixture was then poured into water and extracted with CH2Cl2 (2×25 ml). Combined organic layer was dried over anhyd. Na2SO4, concentrated under vacuum and the crude product was chromatographed over silica gel using benzene-hexane (50:50) as eluant to furnish amino functionalized 1,2,4-trioxane 4eh (upper Rf, 0.29 g, 26.60% yield) and 4ei (lower Rf, 0.44 g, 40.3% yield) as oil.
Example 36
(4-Fluoro-Phenyl)-[3-(1-p-Tolyl-Vinyl)-1,2,5-Trioxa-Spiro[5.5]undec-9-yl]-Amine (Compound 4el, Formula 4, Ar=4-Methylphenyl, R=4-Fluorophenyl)
Acetic acid 1(ml) was added to mixture of trioxane 3e (0.30 g, 1.04 mmol) and 4-fluoroaniline (0.14 g, 1.25 mmol) in dichloromethane (20 ml). Reaction mixture was stirred at room temp for half an hour. Sodium triacetoxyborohydride (0.33 g, 1.55 mmol) was added slowly and it was stirred for 3 h at room temperature. The reaction mixture was then poured into water and extracted with CH2Cl2 (2×20 ml). Combined organic layer was dried over anhyd. Na2SO4, concentrated under vacuum and the crude product was chromatographed over silica gel using benzene-hexane (50:50) as eluant to furnish amino functionalized 1,2,4-trioxane 4el as an inseparable mixture of diastereomers as oil (0.30 g, 75.1% yield).
Example 37
[3-(1-p-Tolyl-Vinyl)-1,2,5-Trioxa-Spiro[5.5]undec-9-yl]-(4-Trifluoromethyl-Phenyl)-Amine (Compound 4eo, Formula 4, Ar=4-Methylphenyl, R=4-Trifluoromethylphenyl)
Acetic acid (1 ml) is added to mixture of trioxane 3e (0.30 g, 1.04 mmol) and 4-trifluoromethyloaniline (0.21 g, 1.30 mmol) in dichloromethane (20 ml). Reaction mixture was stirred at room temp for half an hour. Sodium triacetoxyborohydride (0.33 g, 1.55 mmol) was added slowly and was stirred for 3 h at room temperature. Reaction mixture was then poured into water and extracted with CH2Cl2 (2×20 ml). Combined organic layer was dried over anhyd. Na2SO4, concentrated under vacuum. Crude product was chromatographed over silica gel using benzene-hexane (50:50) as eluant to furnish amino functionalized 1,2,4-trioxane 4eo as an inseparable mixture of diastereomers (0.22 g, 48.7% yield), m.p. 104–106° C.
The following amino functionalized 1,2,4-trioxanes 4ag, 4bf, 4bg, 4bh, 4bi, 4cc, 4cd, 4ce, 4cf, 4cg, 4ch, 4cm, 4cn, 4co, 4df, 4dg, 4dh, 4di, 4dj, 4dk, 4dl, 4dn, 4ej, 4ek, 4em and 4en were prepared following the above procedure (Scheme 1, Table 1).










TABLE 1





S.


%


No.
Amino Functionalized Trioxane
m.p.
yield


















1.
[3-(1-Phenyl-vinyl)-1,2,5-trioxa-spiro[5.5]undec-
oil
43.6



9-yl]-(2-trifluoromethyl-phenyl)-amine (com-



pound 4ag, Formula 4, Ar = phenyl, R = 2-



trifluoromethylphenyl).


2.
[3-(1-Biphenyl-4-yl-vinyl)-1,2,5-trioxa-spiro[5.5]
oil
78.7



undec-9-yl]-(4-fluoro-phenyl)-amine (compound



4bf, Formula 4, Ar = 4-biphenyl, R = 4-fluoro-



phenyl).


3.
[3-(1-Biphenyl-4-yl-vinyl)-1,2,5-trioxa-spiro[5.5]
oil
43.5



undec-9-yl]-(2-trifluoromethyl-phenyl)-amine



(compound 4bg, Formula 4, Ar = 4-biphenyl, R =



2-trifluoromethylphenyl).


4.
[3-(1-Biphenyl-4-yl-vinyl)-1,2,5-trioxa-spiro[5.5]
oil
58.1



undec-9-yl]-(3-trifluoromethyl-phenyl)-amine



(compound 4bh, Formula 4, Ar = 4-biphenyl, R =



3-trifluoromethylphenyl).


5.
[3-(1-Biphenyl-4-yl-vinyl)-1,2,5-trioxa-spiro[5.5]
oil
44.8



undec-9-yl]-(4-trifluoromethyl-phenyl)-amine



(compound 4bi, Formula 4, Ar = 4-biphenyl, R =



4-trifluoromethylphenyl).


6.
(4-chloro-phenyl-{3-[1-(4-Chloro-phenyl)-vinyl]-
130–
26.6



1,2,5-trioxa-spiro[5.5]undec-9-yl}-amine (com-
132° C.



pound 4cc (upper Rf), Formula 4, Ar = 4-chloro-



phenyl, R = 4-chlorophenyl).


7.
(4-chloro-phenyl-{3-[1-(4-Chloro-phenyl)-vinyl]-
138–
40.3



1,2,5-trioxa-spiro[5.5]undec-9-yl}-amine (com-
140° C.



pound 4cd (lower Rf), Formula 4, Ar = 4-chloro-



phenyl, R = 4-chlorophenyl).


8.
{3-[1-(4-Chloro-phenyl)-vinyl]-1,2,5-trioxa-
oil
30.9



spiro[5.5]undec-9-yl}-(3,5-dichloro-phenyl)-



amine (compound 4ce (upper Rf), Formula 4,



Ar = 4-chlorophenyl, R = 3,5-dichlorophenyl).


9.
{3-[1-(4-Chloro-phenyl)-vinyl]-1,2,5-trioxa-
oil
47.4



spiro[5.5]undec-9-yl}-(3,5-dichloro-phenyl)-



amine (compound 4cf (lower Rf), Formula 4,



Ar = 4-chlorophenyl, R = 3,5-dichlorophenyl).


10.
N-(4-{3-[1-(4-Chloro-phenyl)-vinyl]-1,2,5-trioxa-
88–
83.9



spiro[5.5]undec-9-ylamino}-phenyl)-acetamide
90° C.



(compound 4cg, Formula 4, Ar = 4-chlorophenyl,



R = 4-acetylaminophenyl).


11.
{3-[1-(4-Chloro-phenyl)-vinyl]-1,2,5-trioxa-
oil
77.7



spiro[5.5]undec-9-yl}-naphthalen-1-yl-amine



(compound 4ch, Formula 4, Ar = 4-chlorophenyl,



R = 1-naphthyl).


12.
{3-[1-(4-Chloro-phenyl)-vinyl]-1,2,5-trioxa-
oil
71.2



spiro[5.5]undec-9-yl}-(4-fluoro-phenyl)-amine



(compound 4cm, Formula 4, Ar = 4-chloro-



phenyl, R = 4-fluorophenyl).


13.
{3-[1-(4-Chloro-phenyl)-vinyl]-1,2,5-trioxa-
oil
37.4



spiro[5.5]undec-9-yl}-(2-trifluoromethyl-phenyl)-



amine (compound 4cn, Formula 4, Ar = 4-chloro-



phenyl, R = 2-trifluoromethylphenyl).


14.
{3-[1-(4-Chloro-phenyl)-vinyl]-1,2,5-trioxa-
oil
72.5



spiro[5.5]undec-9-yl}-(3-trifluoromethyl-phenyl)-



amine (compound 4co, Formula 4, Ar = 4-chloro-



phenyl, R = 3-trifluoromethylphenyl).


15.
{3-[1-(4-Methoxy-phenyl)-vinyl]-1,2,5-trioxa-
oil
73.4



spiro[5.5]undec-9-yl}-naphthalen-1-yl-amine



(compound 4df, Formula 4, Ar = 4-methoxy-



phenyl, R = 1-naphthyl).


16.
Biphenyl-2-yl-{3-[1-(4-methoxy-phenyl)-vinyl]-
oil
35.4



1,2,5-trioxa-spiro[5.5]undec-9-yl}-amine (com-



pound 4dg (upper Rf), Formula 4, Ar = 4-



methoxyphenyl, R = 2-biphenyl).


17.
Biphenyl-2-yl-{3-[1-(4-methoxy-phenyl)-vinyl]-
oil
52.1



1,2,5-trioxa-spiro[5.5]undec-9-yl}-amine (com-



pound 4dh (lower Rf), Formula 4, Ar = 4-



methoxyphenyl, R = 2-biphenyl).


18.
Biphenyl-4-yl-{3-[1-(4-methoxy-phenyl)-vinyl]-
124–
58.4



1,2,5-trioxa-spiro[5.5]undec-9-yl}-amine (com-
126° C.



pound 4di, (Formula 4, Ar = 4-methoxyphenyl,



R = 4-biphenyl).


19.
9H-Fluoren-3-yl)-{3-[1-(4-methoxy-phenyl)-
166–
75.7



vinyl]-1,2,5-trioxa-spiro[5.5]undec-9-yl}-amine
168° C.



(compound 4dj, Formula 4, Ar = 4-methoxy-



phenyl, R = 2-fluorene).


20.
(4-Fluoro-phenyl)-{3-[1-(4-methoxy-phenyl)-
oil
60.2



vinyl]-1,2,5-trioxa-spiro[5.5]undec-9-yl}-amine



(compound 4dk, Formula 4, Ar '2 4-methoxy-



phenyl, R = 4-fluorophenyl).


21.
{3-[1-(4-Methoxy-phenyl)-vinyl]-1,2,5-trioxa-
oil
36.4



spiro[5.5]undec-9-yl}-(2-trifluoromethyl-



phenyl)-amine (compound 4dl, Formula 4, Ar =



4-methoxyphenyl, R = 2-trifluoromethylphenyl).


22.
{3-[1-(4-Methoxy-phenyl)-vinyl]-1,2,5-trioxa-
oil
40.6



spiro[5.5]undec-9-yl}-(4-trifluoromethyl-



phenyl)-amine (compound 4dn, Formula 4, Ar =



4-methoxyphenyl, R = 4-trifluoromethylphenyl).


23.
Biphenyl-4-yl-[3-(1-p-tolyl-vinyl)-1,2,5-trioxa-
123–
70.5



spiro[5.5]undec-9-yl]-amine (compound 4ej,
124° C.



Formula 4, Ar = 4-methylphenyl, R = 4-



biphenyl).


24.
(9H-Fluoren-3-yl)-[3-(1-p-tolyl-vinyl)-1,2,5-
152–
85.8



trioxa-spiro[5.5]undec-9-yl]-amine (compound
154° C.



4ek, Formula 4, Ar = 4-methylphenyl, R =



2-fluorene).


25.
[3-(1-p-Tolyl-vinyl)-1,2,5-trioxa-spiro[5.5]undec-
oil
40.8



9-yl]-(2-trifluoromethyl-phenyl)-amine (com-



pound 4em, Formula 4, Ar = 4-methylphenyl,



R = 2-trifluoromethylphenyl).


26.
[3-(1-p-Tolyl-vinyl)-1,2,5-trioxa-spiro[5.5]undec-
oil
65.2



9-yl]-(3-trifluoromethyl-phenyl)-amine (com-



pound 4en, Formula 4, Ar = 4-methylphenyl,



R = 3-trifluoromethylphenyl).









Antimalarial Activity

The antimalarial activity of the test compounds was evaluated in rodent using multidrug resistant strain of Plasmodium yoelii Nigeriensis in Swiss mice.
General Procedure
Random bred Swiss mice of either sex (20±2 gm) were inoculated intraperitoneally with 1×105 P. yoelii (MDR) parasites on day zero. Treatments with test compounds were administered to group of 5 mice each at different dose levels ranging between 12–96 mg/kg/day. Treatment was administered in groundnut oil intramuscularly and orally for 4 consecutive days (day 0–3). Blood smears from experimental mice were observed on day 4 and 7, day 10 and thereafter at regular intervals till day 28 or death of animal. Parasitaemia level on day 4 was compared with vehicle control group and percent suppression of parasitaemia in treated groups was calculated. For determining curative dose of a compound treated mice were observed till day 28. The dose at which no parasitaemia develop during the observation period was reported as curative dose. Antimalarial data is summarized in Table 2.







TABLE 2







ANTIMALARIAL ACTIVITY OF TRIOXANES AGAINST P. Yoelii


IN MICE.












Dose

% Suppression
Mice


Compound
(mg/kg/day)
Route
on Day 4a
alive on day-28














3a
96
im
99
0/5


3b
96
im
100
1/5


3c
96
oral
100
0/5


4aa
96
oral
100
5/5



96
im
71
2/5



48
oral
100
3/5


4ab
96
oral
100
5/5



48
oral
100
0/5


4ac
96
oral
100
5/5



96
im
74
0/5



48
oral
100
5/5



24
oral
100
 4/10



12
oral
100
0/5


4ad
96
oral
100
5/5



48
oral
100
5/5



24
oral
100
 6/10



12
oral
99
0/5


4ae
96
oral
100
5/5


4ah
96
oral
100
5/5



96
im
83
0/5



48
oral
100
5/5



24
oral
100
1/5


4ba
96
oral
100
5/5


4bg
96
oral
100
2/5


4cb
48
oral
100
5/5


4cc
48
oral
100
5/5



24
oral
100
4/5


4cd
48
oral
100
5/5



24
oral
100
4/5


4ce
48
oral
100
4/5



24
oral
100
2/5


4cf
48
oral
100
2/5



24
oral
100
1/5


4ch
96
oral
100
2/5


4cj
48
oral
100
5/5



24
oral
100
2/5


4ck
96
oral
100
2/5


4co
96
oral
100
5/5



96
im
73
0/5



48
oral
100
5/5



24
oral
100
3/5


4da
96
oral
100
0/5



96
im
81
0/5


4dc
96
oral
100
5/5



96
im
100
4/5



48
oral
100
5/5



24
oral
100
4/5


4dd
96
oral
100
4/5


4df
96
oral
100
5/5



48
oral
100
2/5


4dg
48
oral
100
3/5



24
oral
91
1/5


4dh
48
oral
100
5/5



24
oral
100
5/5


4dm
96
oral
100
5/5



96
im
100
0/5



48
oral
100
5/5



24
oral
100
5/5


4ea
96
oral
100
5/5



96
im
96
0/5


4eb
96
oral
100
5/5



96
im
93
0/5


4ec
96
oral
100
5/5


4ed
48
oral
100
4/5



24
oral
95
0/5


4ee
48
oral
100
2/5



24
oral
81
0/5


4eg
96
oral
100
5/5


4ej
96
oral
100
5/5



96
im
69
0/5



48
oral
100
2/5


4en
96
oral
100
3/5


β-Arteether
48
oral
100
5/5



24
oral
100
1/5


Chloroquine
48
oral
100
2/5



24
oral
100
0/5


Vehicle
—
—
—
 0/15


Control





aPercent suppression = [(C − T)/C] × 100; where C = parasitaemia in control group, and T = parasitaemia in treated group.

## Claims

1. A substituted amino functionalized 1,2,4-trioxane of formula 4




wherein Ar represents aryl groups selected from the groups consisting of phenyl, 4-biphenyl, 4-chlorophenyl, 4-methoxyphenyl and 4-methylphenyl and R represents phenyl, 4-methoxyphenyl, 4-chlorophenyl, 3,5-dichlorophenyl, 4-acetylaminophenyl, 1-naphthyl, 2-biphenyl, 4-biphenyl, 2-fluorene, 4-fluorophenyl, 2-trifluoromethylphenyl, 3-trifluoromethylphenyl and 4-trifluoromethylphenyl.

2. Compound of formula 4 with structural formula 4aa–4ai shown below:

3. Compound of formula 4 with structural formula 4ba–4bi shown below:

4. Compound of formula 4 with structural formula 4ca–4cp shown below:

5. Compound of formula 4 with structural formula 4da–4dn shown below:

6. Compound of formula 4 with structural formula 4ea–4eo shown below:

7. Method for the preparation of a substituted amino functionalized-1,2,4-trioxane of formula 4




wherein Ar represents aryl groups selected from the groups consisting of phenyl, 4-biphenyl, 4-chlorophenyl, 4-methoxyphenyl and 4-methylphenyl and R represents phenyl, 4-methoxyphenyl, 4-chlorophenyl, 3,5-dichlorophenyl, 4-acetylaminophenyl, 1-naphthyl, 2-biphenyl, 4-biphenyl, 2-fluorene, 4-fluorophenyl, 2-trifluoromethylphenyl, 3-trifluoromethylphenyl and 4-trifluoromethylphenyl, the process comprising reacting a keto trioxane of formula 3




with an aromatic amine in the presence of NaBH(OAc)3 in CH2Cl2 to furnish amino functionalized 1,2,4-trioxanes of formula 4.

8. A method as claimed in claim 7 wherein the aromatic amine is selected from the group consisting of aniline, 4-methoxyaniline, 4-chloroaniline, 3,5-dichloroaniline, 4-aminoacetanilide, 1-naphthylamine, 2-aminobiphenyl, 4-aminobiphenyl, 2-aminofluorene, 4-fluoroaniline, 2-trifluoromethylaniline, 3-trifluoromethylaniline and 4-trifluoromethylaniline.

9. A method for the preparation of substituted amino functionalized-1,2,4-trioxanes of formula 4




wherein Ar is an aryl group selected from the group consisting of phenyl, 4-biphenyl, 4-chlorophenyl, 4-methoxyphenyl and 4-methylphenyl and R represents phenyl, 4-methoxyphenyl, 4-chlorophenyl, 3,5-dichlorophenyl, 4-acetylaminophenyl, 1-naphthyl, 2-biphenyl, 4-biphenyl, 2-fluorene, 4-fluorophenyl, 2-trifluoromethylphenyl, 3-trifluoromethylphenyl and 4-trifluoromethylphenyl, the method comprising:
(i) photooxygenating an allylic alcohol of formula 1 by passing oxygen gas or air in a solution of the alcohol in an organic solvent and in the presence of a dye and a light source which provides visible light, for a period of 4 h, to obtain a β-hydrohydroperoxide of formula 2




(ii) reacting the hydroperixodes of formula 2 in situ with 1,4-cyclohexanedione in the presence of an acid catalyst to give a trioxane of formula 3;




 reacting the keto trioxane of formula 3 with an aromatic amine in the presence of NaBH(OAc)3 in CH2Cl2 to obtain amino functionalized 1,2,4-trioxane of formula 4.

10. A method as claimed in claim 9 wherein the aromatic amine is selected from the group consisting of aniline, 4-methoxyaniline, 4-chloroaniline, 3,5-dichloroaniline, 4-aminoacetanilide, 1-naphthylamine, 2-aminobiphenyl, 4-aminobiphenyl, 2-aminofluorene, 4-fluoroaniline, 2-trifluoromethylaniline, 3-trifluoromethylaniline and 4-trifluoromethylaniline.

11. A method of treating a subject having malaria, comprising administering to the subject a pharmaceutically effective amount of a composition containing substituted amino functionalized-1,2,4-trioxanes of formula 4,




wherein Ar represents aryl groups selected from the groups consisting of phenyl, 4-biphenyl, 4-chlorophenyl, 4-methoxyphenyl and 4-methylphenyl and R represents phenyl, 4-methoxyphenyl, 4-chlorophenyl, 3,5-dichlorophenyl, 4-acetylaminophenyl, 1-naphthyl, 2-biphenyl, 4-biphenyl, 2-fluorene, 4-fluorophenyl, 2-trifluoromethylphenyl, 3-trifluoromethylphenyl and 4-trifluoromethylphenyl and a pharmaceutically acceptable carrier.

12. A method as claimed in claim 11 wherein the composition is administered intramuscularly or orally.

13. A method as claimed in claim 11 wherein the pharmaceutically acceptable amount of compound of formula 4 is in the range of 12 to 96 mg of compound of formula 4 per kilogram of body weight of subject per day.

14. A method as claimed in claim 11 wherein the subject is a human being.