# Patent

## Title
Compounds and compositions for delivering active agents

## Abstract

Carrier compounds and compositions therewith which are useful in the delivery of active agents are provided. Methods of administration and preparation are provided as well.

## Background
This is a continuation of application Ser. No. 09/746,548, filed Dec. 19, 2000, now abandoned which is a divisional of application Ser. No. 08/796,336, filed Feb. 7, 1997 now U.S. Pat. No. 6,358,504. Each of these prior applications are hereby incorporated herein by reference, in its entirety.


FIELD OF THE INVENTION
The present invention relates to compounds for delivering active agents, and particularly biologically or chemically active agents. These compounds are used as carriers to facilitate the delivery of a cargo to a target. The carrier compounds are well suited to form non-covalent mixtures with biologically-active agents for oral administration to animals. Methods for the preparation administration of such compositions are also disclosed.
BACKGROUND OF THE INVENTION
Conventional means for delivering active agents are often severely limited by biological, chemical, and physical barriers. Typically, these barriers are imposed by the environment through which delivery occurs, the environment of the target for delivery, or the target itself. Biologically or chemically active agents are particularly vulnerable to such barriers.
For example in the delivery to animals of biologically active or chemically active pharmacological and therapeutic agents, barriers are imposed by the body. Examples of physical barriers are the skin and various organ membranes that must be traversed before reaching a target. Chemical barriers include, but are not limited to, pH variations, lipid bi-layers, and degrading enzymes.
These barriers are of particular significance in the design of oral delivery systems. Oral delivery of many biologically or chemically active agents would be the route of choice for administration to animals if not for biological, chemical, and physical barriers such as varying pH in the gastro-intestinal (GI) tract, powerful digestive enzymes, and active agent impermeable gastro-intestinal membranes. Among the numerous agents which are not typically amenable to oral administration are biologically or chemically active peptides, such as calcitonin and insulin; polysaccharides, and in particular mucopolysaccharides including, but not limited to, heparin; heparinoids; antibiotics; and other organic substances. These agents are rapidly rendered ineffective or are destroyed in the gastro-intestinal tract by acid hydrolysis, enzymes, or the like.
Earlier methods for orally administering vulnerable pharmacological agents have relied on the co-administration of adjuvants (e.g., resorcinols and non-ionic surfactants such as polyoxyethylene oleyl ether and n-hexadecylpolyethylene ether) to increase artificially the permeability of the intestinal walls, as well as the co-administration of enzymatic inhibitors (e.g., pancreatic trypsin inhibitors, diisopropylfluorophosphate (DFF) and trasylol) to inhibit enzymatic degradation.
Liposomes have also been described as drug delivery systems for insulin and heparin. See, for example, U.S. Pat. No. 4,239,754; Patel et al. (1976), FEBS Letters, Vol. 62, pg. 60; and Hashimoto et al. (1979), Endocrinology Japan, Vol. 26, pg. 337.
However, broad spectrum use of such drug delivery systems is precluded because: (1) the systems require toxic amounts of adjuvants or inhibitors; (2) suitable low molecular weight cargos, i.e. active agents, are not available; (3) the systems exhibit poor stability and inadequate shelf life; (4) the systems are difficult to manufacture; (5) the systems fail to protect the active agent (cargo); (6) the systems adversely alter the active agent; or (7) the systems fail to allow or promote absorption of the active agent.
More recently, microspheres of artificial polymers of mixed amino acids (proteinoids) have been used to deliver pharmaceuticals. For example, U.S. Pat. No. 4,925,673 describes drug-containing proteinoid microsphere carriers as well as methods for their preparation and use. These proteinoid microspheres are useful for the delivery of a number of active agents.
There is still a need in the art for simple, inexpensive delivery systems which are easily prepared and which can deliver a broad range of active agents.
SUMMARY OF THE INVENTION
Compounds and compositions which are useful in the delivery of active agents are provided. These compositions include at least one active agent, preferably a biologically or chemically active agent, and at least one of the following compounds 1–193, or salts thereof.

















1


 


6-N-(3,5-dichloro-2-hydroxybenzoyl)aminocaproic acid


 






2


 


8(2-aminobenzoylamino)caprylic acid


 






3


 


8(2-trifluoromethoxy)benzoylamino caprylic acid


 






4


 


N-(2-hydroxybenzoyl)isonipecotic acid


 






5


 


4-[4-(2-aminobenzoylamino)phenyl]butyrylhydroxamic acid


 






6


 


4-(4-(pentafluorobenzoyl)aminophenyl)butyric acid


 






7


 


4-(4-(3-anisoyl)aminophenyl)butyric acid


 






8


 


8-(3-anisoyl)aminocaprylic acid


 






9


 


4-(4-(phenoxyacetyl)aminophenyl)butyric acid


 






10


 


4-(4-(2-nitrobenzenesulfonyl)aminophenyl)butyric acid


 






11


 


8-(2-nitrobenzenesulfonyl)aminocaprylic acid


 






12


 


6-(4-(salicyloyl)aminoiphenyl)hexanoic acid


 






13


 


8-(2-methoxybenzoyl)amino caprylic acid


 






14


 


2-[4-Salicyloylamino)phenyl]ethyl methyl sulfone


 






15


 


1-Salicyloyl-2-succinyl hydrazide


 






16


 


3-(4-(2,5-dimethoxycinnamoyl)aminophenyl)propionic acid


 






17


 


4-(4-(2,5-dimethoxycinnamoyl)aminophenyl)butyric acid


 






18


 


1-salicyloyl-2-glutaryl hydrazide


 






19


 


Succinyl-4-aminosalicyclic acid


 






20


 


8-(Phenoxyacetylamino)caprylic acid


 






21


 


8-(2-pyrazinecarbonyl)aminocaprylic acid


 






22


 


4-(4-(2-pyrazinecarbonyl)aminophenyl)butyric acid


 






23


 


6-(4-(N-2-Nitrobenzoyl)aminophenyl)hexanoic acid


 






24


 


6-(4-(N-2-aminobenzoyl)aminophenyl)hexanoic acid


 






25


 


4-(4-(2-(3-carboxyl)pyrazinecarboxyl)aminophenyl)butyric acid


 






26


 


4-(2-Nitrobenzoyl)aminophenylsuccinic acid


 






27


 


8-(2-(trifluoromethoxy)benzoyl)aminocaprylic acid


 






28


 






29


 


8-(Benzylcarbonylamino)caprylic acid


 






30


 


8-(phenylcarbonylamino)caprylic acid


 






31


 


2-[4-(2-Methoxybenzoylamino)phenyl]ethyl H2PO4


 






32


 


1-salicyloyl-2-suberyl hydrazide


 






33


 


4-(4-benzoylcarbonylkaminophenyl)butyric acid


 






34


 


4-(4-(2-hydroxynicotinoyl)aminophenyl)butyric acid


 






35


 


9-Salicyloylaminononanic acid


 






36


 


4-(4-phenyloxycarbonylaminophenyl)butyric acid


 






37


 


3-(2-methoxybenzoylamino)-1-propanol


 






38


 


8-(2-Hydroxynicotinoyl)aminocaprylic acid


 






39


 


6-(2-methoxybenzoyl)amino nicotinic acid


 






40


 


salicyloylglycine


 






41


 


4-(1-(2-pyrimidyl)piperazinoyl)butyric acid


 






42


 


8-(chromone-3-carbonyl)aminocaprylic acid


 






43


 


8-(vinylbenzoyl)aminocaprylic acid


 






44


 


4-(4-(chromone-3-carbonyl)aminophenyl)butyric acid


 






45


 


8-cinnamoylaminocaprylic acid


 






46


 


5-(N-salicyloylamino)valeric acid


 






47


 


9-(2-hydroxybenzamido)nonanic acid


 






48


 


N-(4-saliyloylamino)-6-caproic acid


 






49


 


4′-flavonic acid


 






50


 


11-cinnamoylaminoundecanoic acid


 






51


 


4-octylamino-3-hydroxybenzoic acid


 






52


 


(3Phenyl2,3dihydroxypropanoyl)8aminocaprylic acid


 






53


 


8-[N-(3-coumarincarbonyl)]aminocaprylic acid


 






54


 


8-[N-(4-chlorobenzoyl)aminocaprylic acid


 






55


 


8-[N-3-fluorobenzoyl)]aminocaprylic acid


 






56


 


8-(N-2,5-Dihydroxybenzoyl)aminocaprylic acid


 






57


 


8-(N-2,3-Dimethoxybenzoyl)aminocaprylic acid


 






58


 


8-(N-2,3-Dimethoxybenzoyl)aminocaprylic acid


 






59


 


8-(N-2,5-Dimethoxybenzoyl)aminocaprylic acid


 






60


 


8-(N-3,5-Diacetylbenzoyl)aminocaprylic acid


 






61


 


8-(N-4-Hydroxybenzoyl)aminocaprylic acid (dimer)


 






62


 


8-(N-2,4-Dihydroxybenzoyl)aminocaprylic acid


 






63


 


10-(N-2-Methoxyanilino)sebacic acid


 






64


 


10-(N-2-Methoxyanilino)sebacic acid


 






65


 


2-Methoxybenzenaminodecanoic acid


 






66


 


8-(N-benzoyl)aminocaprylic acid


 






67


 


8-(N-2-Hydroxy-4-methoxybenzoyl)aminocaprylic acid


 






68


 


8-[N-(4-fluorobenzoyl)]aminocaprylic acid


 






69


 


8-[N-(3-bromobenzoyl)]aminocaprylic acid


 






70


 


8-(4-(1,2-dihydroxyethyl)benzoyl)aminocaprylic acid


 






71


 


8-[N-(4-bromobenzoyl)]aminocaprylic acid


 






72


 


8-[N-(4-iodobenzoyl)]aminocaprylic acid


 






73


 


4-{4-[N-(2-iodobenzoyl)aminophenyl]}butyric acid


 






74


 


4-{4-[N-(1-hydroxy-2-naphthoyl)aminophenyl]}butyric acid


 






75


 


4-(4-(2,4-dimethoxybenzoyl)aminophenyl)butyric acid


 






76


 


4-(o-anisoyl)aminophenylacetic acid


 






77


 


3-[4-(2,4-dimethoxybenzoyl) aminophenyl] propionic acid


 






78


 


4-{4-[N-(4-iodobenzoyl)] aminophenyl} butyric acid


 






79


 


3-[4-(2,3-dimethoxybenzoyl) aminophenyl] propionic acid


 






80


 


4{4-[N-2-bromobenzoyl)] aminophenyl} butyric acid


 






81


 


4{4-[N-3-bromobenzoyl] aminophenyl} butyric acid


 






82


 


8-(N-3,5-Dihydroxybenzoyl)aminocaprylic acid


 






83


 


8-(N-3,5-Dimethoxy 4-hydroxybenzoyl)aminocaprylic acid


 






84


 


8-(N-2-6-Dimethopxybenzoyl)aminocaprylic acid


 






85


 


4-{4-[N-(4-bromobenzoyl)aminophenyl}butyric acid


 






86


 


8-(2-hydroxy-4-chlorobenzoyl)aminocaprylic acid


 






87


 


8-(N-2,6-Dihydroxybenzoyl)aminocaprylic acid


 






88


 


8-(N-2-Hydroxy6-methoxybenzoyl)aminocaprylic acid


 






89


 


8-(5-chloro-o-anisoyl)aminocaprylic acid


 






90


 


4-(4-(2,3-dimethoxybenzoyl)aminophenyl)butyric acid


 






91


 


4-(4-(5-chloro-o-anisoyl)aminophenyl)butyric acid


 






92


 


4-(4-(4-chloro-o-anisoyl)aminophenyl)butyric acid


 






93


 


8-(4-chloro-o-anisoyl)aminocaprylic acid


 






94


 


3-(4-(2,5-dimethoxybenzoyl)aminophenyl)propionic acid


 






95


 


4-{N-[4-(3-iodobenzoyl)aminophenyl]}butyric acid


 






96


 


7-cinnamoylaminoheptanoic acid


 






97


 


8-N-(3-iodobenzoyl)aminocaprylic acid


 






98


 


8-N-(4-methoxy-3-nitrobenzoyl)aminocaprylic acid


 






99


 


8-N-(2-methoxy-4-nitrobenzoyl)aminocaprylic acid


 






100


 


4-{N-[4-(2-methoxy-4-nitrobenzoyl)aminophenyl]}butyric acid


 






101


 


4-(4-(2,5-dimethoxybenzoyl)aminophenyl)butyric acid


 






102


 


8-(N-2-hydroxy-5-bromobenzoyl)aminocaprylic acid


 






103


 


3-indolebutyric acid


 






104


 






105


 






106


 






107


 


4-(4-(2,6-dimethoxybenzoyl)aminophenylbutyric acid


 






108


 


4-[4-N-(4-methoxy-3-nitrobenzoyl)aminophenyl]butyric acid


 






109


 


8-(N-2-hydroxy-5-chlorobenzoyl)aminocaprylic acid


 






110


 


8-(N-2-hydroxy-5-iodobenzoyl)aminocaprylic acid


 






111


 


8-(3-hydroxy-2-naphthoyl)aminocaprylic acid


 






112


 


8-(N-2-hydroxy-4-nitrobenzoyl)aminocaprylic acid


 






113


 


4-[-N-(2-hydroxy-4-bromobenzoyl)aminophenyl]butyric acid


 






114


 


8-(N-2,3-Dihydroxybenzoyl)aminocaprylic acid


 






115


 


8-(N-3-methylsalicyloyl)aminocaprylic acid


 






116


 


8-(N-5-methylsalicyloyl)aminocaprylic acid


 






117


 


9-(cinnamoylamino)nonanionic acid


 






118


 


4-(4-(2-chloro-5-nitrobenzoyl)aminophenyl)butyric acid


 






119


 


4-{-[N-(2-hydroxy-5-iodobenzoyl)]aminophenyl}butyric acid


 






120


 


N-2-bitrophenyl-N′-(8-octanoic acid) urea


 






121


 


N-(2-merthoxy-5-nitrophenyl) sebacoyl amide acid


 






122


 


8-[N-(2-acetoxy-3,5-dichlorobenzoyl)]aminocaprylic acid


 






123


 


8-[N-(2-acetoxy-3,5-dibromobenzoyl)]aminocaprylic acid


 






124


 


8-N-(2-chloro-6-fluorobenzoyl)aminocaprylic acid


 






125


 






126


 


8-N-(4-hydroxy-3-nitrobenzoyl)caprylic acid


 






127


 


4-(4-Salicyloylaminophenyl)-4-oxobutyric acid


 






128


 


12-cinnamoyldodecanoic acid


 






129


 


4-{4-[N-(3-hydroxy-2-naphthoyl)aminophenyl]}butyric acid


 






130


 


8-(4-chloro-3-bitrobenzoyl)aminocaprylic acid


 






131


 


8-(2-chloronicotinoyl)aminocaprylic acid


 






132


 


8-(2-chloro-5-nitrobenzoyl)aminocaprylic acid


 






133


 


4-(4-phthalimidophenyl)butyric acid


 






134


 


4-{4-[N-(3-hydroxy-2-naphthoyl)aminophenyl]}propanoic acid


 






135


 


3-(4-(2,6-dimethoxybenzoyl)aminophenyl)propaionic acid


 






136


 


8-(N-2-hydroxy-3,5-diidobenzoyl)aminocaprylic acid


 






137


 


8-(N-2-chloro-4-fluorobenzoyl)aminocaprylic acid


 






138


 


8-(2-(1,2-dihydroisoindole-1-one))octanoic acid


 






139


 


8-(N-1-hydroxy-2-naphthoyl)aminocaprylic acid


 






140


 


8-(phthalimido)caprylic acid


 






141


 


1-(4-chloro-2-hydroxyanilino)sebacic acid monoamide


 






142


 


6-(anisoyl)aminocaprylic acid


 






143


 


4-(4-(4-chloro-3-nitrobenzoyl)aminophenyl)butyric acid


 






144


 


11-N-(1-hydroxy-2-naphthoyl)aminoundecanoic acid


 






145


 


Bis(N-2carboxylphenyl-N-(N′-8-octanoic acid)ureal)oxalyl diamide


 






146


 


2-[2-N-(2-chlorobenzoyl)aminoethoxy]ethanol


 






147


 


2-[2-N-(4-chlorobenzoyl)aminoethoxy]ethanol


 






148


 


4-(2-methoxybenzoyl)amino 3-carboxysulfoxide


 






149


 


4-(2-methoxybenzoyl)amino 3-carboxypropylsulfone


 






150


 


4-(4-(3-hydroxyphthalimido)phenyl)butyric acid


 






151


 


2-[2-N-(2-methoxybenzoyl)aminoethoxy)]ethanol


 






152


 


2-[2-N-(3-chlorobenzoyl)aminoethoxy)]ethanol


 






153


 


Bis(N-2-carboxyphenyl-N-(N′-3(4-aminophenyl)propionic acid)ureal)oxalyl diamide


 






154


 


trans-4-(2-aminobenzamidomethyl)cyclohexamecarboxylic acid


 






155


 


11-N-(3,5-dichloro-2-hydroxybenzoyl)aminoundecanoic acid


 






156


 


2-[N-(2-bromobenzoyl)aminoethoxy]ethanol


 






157


 


7-N-(3,5-dichloro-2-hydroxybenzoyl)aminoheptanoic acid


 






158


 


N-[3,5-dichloro-2-hydroxybenzoyl-4(4-aminophenyl)]butyric acid


 






159


 


trans-4-(N-salicyloylaminomethyl)cyclohexane carboxylic acid


 






160


 


N-[3,5-dichloro-2-hydroxybenzoyl-3-(4-aminophenyl)]propionic acid


 






161


 


12-N-(3,5-dichloro-2-hydroxybenzoyl)aminododecanoic acid


 






162


 


N-(2-hydroxy-4-carboxy)-6-heptenamide


 






163


 


N-(2-bromobenzoyl)morpholine


 






164


 


8-N-cyclohexanoylaminocaprylic acid


 






165


 


2-[N-(2-iodobenzoyl)aminoethoxy]ethanol


 






166


 


5-(4-chloro-2-hydroxyanilinocarbonyl)valeric acid


 






167


 


8-(2-hydroxyphenoxy)aminocaprylic acid


 






168


 


N-Saliyloyl-5-(3-aminophenyl)valeric acid


 






169


 


4-(4-(2-ethoxybenzoyl)aminophenyl)butyric acid


 






170


 


9-[2-(3-hydroxy)pyridylaminocarbonyl] nonanionic acid


 






171


 


7-(2-hydroxyphenoxyacetyl)aminocaprylic acid


 






172


 


2-[N-(2-hydroxybenzoylamino)ethoxy]ethanol


 






173


 


4-[N-(3,5-dichloro-2-hydroxybenzoyl)]aminophenylacetic acid


 






174


 


8-(2-hydroxy-5-chloroanilinocarbonyl)octanoic acid


 






175


 


N-salicyloyl-5-(4-aminophenyl)valeric acid


 






176


 


9-(2-hydroxy-5-methylanilinocarbonyl)nonanionic acid


 






177


 


5-(2-hydroxy-5-methylanilinocarbonyl)valeric acid


 






178


 


8-(pentafluorobenzoyl)aminocaprylic acid


 






179


 


3-(3-salicyloyl)aminophenyl)propionic acid


 






180


 


8-(2-ethoxybenzoyl)aminocaprylic acid


 






181


 


4-(4-(2-Dimethylamino benzoic)aminophenyl]butyric acid


 






182


 


8-(3-Phenoxypropionylamino)caprylic acid


 






183


 


4-(Salicyloyl)aminophenylethyltetrazole


 






184


 


8(-(4(N-Salicyloyl-4aminophenyl)butyric)aminocaprylic acid [sic]


 






185


 


4-(4-(N-(2-Fluorocinnamoyl))aminophenyl) butyric


 






186


 


4-(4-(N-8(N-Salicyloyl)aminocaprylic)aminophenyl)butyric acid


 






187


 


8-(p-anisoyl)aminocaprylic acid


 






188


 


8-(4-Hydroxybenzoyl)aminocaprylic acid


 






189


 


8-(3-Hydroxybenzoyl)aminocaprylic acid


 






190


 


8-(3,4,5-Trimethoxybenzoyl)aminocaprylic acid


 






191


 


8-(N-4-Methylsalicyloyl)aminocaprillic acid [sic]


 






192


 


N-10-(2-hydroxy-5-nitroanilino)decanoic acid


 






193


 


4-(4-(2-chloronicotinoyl)aminophenyl)butyric acid









Compositions comprising the carrier compounds discussed above and active agents are effective in delivering active agents to selected biological systems.


DETAILED DESCRIPTION OF THE INVENTION
The specific compositions of the present invention include an active agent and a carrier. These compositions may be used to deliver various active agents through various biological, chemical, and physical barriers and are particularly suited for delivering active agents which are subject to environmental degradation. The compositions of the subject invention are particularly useful for delivering or administering biologically or chemically active agents to any animals such as birds including, but not limited to, chickens; mammals, such as primates and particularly humans; and insects.
Other advantages of the present invention include the use of easy to prepare, inexpensive raw materials. The compositions and the formulation methods of the present invention are cost effective, simple to perform, and amenable to industrial scale up for commercial production.
Subcutaneous, sublingual, and intranasal coadministration of an active agent, such as, for example, recombinant human growth hormone (rhGH); salmon calcitonin; heparin, including, but not limited to, low molecular weight heparin; parathyroid hormone; and compounds in compositions as described herein result in an increased bioavailability of the active agent compared to administration of the active agent alone.
Active Agents
Active agents suitable for use in the present invention include biologically or chemically active agents, chemically active agents, including, but not limited to, fragrances, as well as other active agents such as, for example, cosmetics.
Biologically or chemically active agents include, but are not limited to, pesticides, pharmacological agents, and therapeutic agents. For example, biologically or chemically active agents suitable for use in the present invention include, but are not limited to, peptides, and particularly small peptides; hormones, and particularly hormones which by themselves do not or only a fraction of the administered dose passes through the gastro-intestinal mucosa and/or are susceptible to chemical cleavage by acids and enzymes in the gastro-intestinal tract; polysaccharides, and particularly mixtures of muco-polysaccharides; carbohydrates; lipids; or any combination thereof. Further examples include, but are not limited to, human growth hormones; bovine growth hormones; growth releasing hormones; interferons; interleukin-1; insulin; heparin, and particularly low molecular weight heparin; calcitonin; erythropoietin; atrial naturetic factor; antigens; monoclonal antibodies; somatostatin; adrenocorticotropin, gonadotropin releasing hormone; oxytocin; vasopressin; cromolyn sodium (sodium or disodium chromoglycate); vancomycin; desferrioxamine (DFO); parathyroid hormone anti-microbials, including, but not limited to anti-fungal agents; or any combination thereof.
Carriers
Although compounds 1–193 above have been found to act as carriers for the oral delivery of biologically or chemically active agents, special mention is made of compounds 9, 35, 64, 67, 79, 102, 109, 111, 117, 122, 136, and 141, above.
Properties of compounds 1–193 are listed in Table 1, below.







TABLE 1







Carrier Properties











Anal. Calculated For
Found
Melting Point
















Compound
C
H
N
S
C
H
N
S
(° C.)



















1
48.8
4.70
4.40

48.81
4.64
4.39




2
64.73
7.97
10.06

64.54
7.81
10.19


3
55.33
5.80
4.03

55.40
5.79
3.96

69–71


4
62.64
6.06
5.62

62.75
6.08
5.51

151–154


5
65.16
6.11
13.40

65.29
6.03
13.29

144–145


6
54.70
3.24
3.75

54.29
3.24
3.54

165–169


7
69.00
6.11
4.47

69.09
6.24
4.43

126–129


8
65.51
7.90
4.78

65.60
8.25
4.83

89–90


9
68.99
6.11
4.47

69.01
6.08
4.47

104–107


10
52.74
4.42
7.69

52.91
4.45
7.49

142–145


11
48.83
5.85
8.14

48.95
5.89
8.02

120–122


12
69.71
6.47
4.28

69.56
6.47
4.38

144–146


13
65.51
7.90
4.77

65.23
7.88
4.72

72.5–74.5


14
60.17
5.36
4.39
10.04
60.09
5.36
4.35
9.99
155–156


15
52.38
4.79
11.11

52.45
4.94
11.08

220–222


16
67.60
5.95
3.94

67.34
6.01
3.91

219–222


17
68.09
6.53
3.78

67.77
6.24
3.81

130–133


18
54.13
5.30
10.52

54.12
5.24
10.54

192.5–











195.5


19
55.26
4.21
7.16

54.48
4.32
6.86

>280











dec


20
65.51
7.90
4.77

65.52
7.90
4.77

75–80


21
58.85
7.21
15.84

58.86
7.16
15.69

120–122


22
63.15
5.30
14.73

63.30
5.43
14.18

197–201


23
64.04
5.66
7.86

64.17
5.67
7.75

188–190


24
69.91
6.88
8.46

69.98
6.79
8.58

131–134


25
58.36
4.56
12.76

58.20
4.63
12.61

138–141


26
56.98
3.94
7.82

56.39
3.92
7.74

221–223


27
55.33
5.80
4.03

55.47
6.10
4.04

70–72


28


29
65.74
7.58
4.79

65.51
7.89
4.78

52–55


30
64.50
7.57
5.02

64.07
7.81
5.40

70–74


31
54.70
5.17
3.99

54.50
4.99
3.95

173–174


32
58.63
5.94
9.12

58.73
6.20
10.34

125–129


33
69.00
6.10
4.47

69.18
6.08
4.54

100–102


34
63.99
5.37
9.33

63.46
5.35
9.06

  218–221c


35
65.5
7.90
4.78

65.37
8.00
4.66

  96–97C


36
68.22
5.72
4.68

67.88
5.65
4.55

134–137


37
63.14
7.23
6.69

63.15
7.29
6.58

53.5–56  


38
60.00
7.14
10.00

59.78
7.31
9.94

135–138


39
61.67
4.41
10.29

61.69
4.41
10.12

>225


40
55.39
4.65
7.18

55.52
4.77
7.30

162.5–











166


41
56.10
6.52
20.14

55.66
6.71
19.69

129–131


42
65.24
6.39
4.23

65.42
6.16
3.78

130–











133.5


43
70.59
7.96
4.84

70.35
8.13
4.79

111–113


44
68.37
4.88
3.99

68.61
4.89
3.79

120–123


45
70.59
7.96
4.84

70.48
7.97
4.71

108–110


46
60.75
6.37
5.90

60.97
6.18
5.80

100.5–











103


47
64.50
7.57
5.02

64.42
7.58
5.01

 97–100


48
64.86
5.98
7.56

64.50
6.01
7.52

165–169


49
72.18
3.76
0.00

72.13
3.84
0.00

>225


50
72.51
8.76
4.23

72.39
8.84
4.12

120–122


51
64.50
7.58
5.01

64.75
7.65
4.69

200.5–











204


52

7.74
4.33


7.82
4.30

88–89


53
65.24
6.39
4.23

65.15
6.46
4.23

93–97


54
60.49
6.77
4.70

60.54
6.76
4.65

114–116


55
64.04
7.17
4.98

63.90
7.11
4.93

105–106


56
61.00
7.17
4.74

60.49
6.92
4.65

146–148


57
63.14
7.79
4.33

63.22
7.82
4.36

59–61


58
63.14
7.79
4.33

63.17
7.86
4.26

102–104


59
63.14
7.79
4.33

63.35
7.68
4.20

89–90


60
60.15
6.64
3.69

59.84
6.66
3.64

112–113


61
65.53
8.85
6.65

65.34
8.73
6.67

89–92


62
61.00
7.17
4.74

60.94
7.12
4.49

104–108


63
66.43
8.20
4.56

66.29
8.23
4.36

77–78


64
65.51
7.90
4.77

65.52
8.06
4.54

97–98


65
69.59
9.28
4.77

69.64
9.35
4.86

62–65


66
68.41
8.04
5.32

68.41
8.06
5.28

88–89


67
62.12
7.49
4.53

61.94
7.45
4.43

98–99


68
64.04
7.17
4.98

64.07
7.16
4.95

106–107


69
52.64
5.89
4.09

52.63
5.85
4.03

109–110


70
63.15
7.74
4.33

63.26
7.90
4.14

 97–100


71
52.64
5.89
4.09

52.67
5.99
3.97

114–115


72
46.31
5.18
3.61

46.25
4.86
3.52

143–144


73
49.89
3.94
3.42

49.92
3.85
3.39

170–171


74
72.19
5.48
4.01

71.51
5.33
3.75

180


75
66.46
6.16
4.08

66.47
6.26
4.06

168.5–











171


76
67.37
5.26
4.91

67.31
5.25
5.07

130–133


77
65.65
5.78
4.26

65.49
6.04
4.26

179–183


78
49.89
3.94
3.42

49.8
3.71
3.29

237–238


79
65.65
5.78
4.26

65.21
6.05
4.24

156–158


80
56.38
4.45
3.87

56.4
4.21
3.91

130–131


81
56.38
4.45
3.87

56.46
4.5
3.84

197–198


82
56.6
7.49
4.4

56.3
7.49
4.14

58–62


83
57.03
8.2
3.91

57.17
7.8
3.7

138–140


84
57.58
7.11
3.95

57.52
7.7
3.94


85
56.38
4.45
3.87

56.31
4.25
3.64

230–231


86
57.42
6.42
4.46

57.14
6.45
4.2

116–117


87
61
7.17
4.74

61.18
7.05
4.65

108–109


88
62.12
7.49
4.53

62.34
7.21
4.39

107–109


89
58.63
6.76
4.27

58.53
6.81
4.2

117–118


90
66.46
6.16
4.08

66.18
6.15
3.84

100–104


91
62.16
5.21
4.03

61.93
4.97
3.86

183–185


92
62.16
5.21
4.03

62.2
5.14
3.98

167–170


93
58.63
6.76
4.27

58.64
6.83
4.19

106–108


94
65.65
5.81
4.25

65.56
5.64
4.2

153–156


95
49.89
3.94
3.42

49.9
3.81
3.18

216–217


96
69.82
7.64
5.09

69.91
7.66
5.02

129–131


97
46.31
5.18
3.61

46.54
4.95
3.64

122–123


98
56.8
6.55
8.28

56.69
6.67
8.1


99
56.8
6.55
8.28

57.37
6.57
8.33

117–118


100
60.33
5.06
7.82

59.98
4.97
7.67

207–209


101
66.46
6.16
4.08

66.37
6.32
3.96

126–128


102
50.29
5.63
3.91

50.14
5.7
3.76

129–131


103
70.93
5.95
6.89

70.94
6.44
6.89


104
65.84
6.14
8.53

65.94
6.19
8.54

228–231


105
64.96
5.77
8.91

64.89
5.82
8.82


106
66.65
6.48
8.18

66.39
6.49
8.05

140–142


107
66.47
6.12
4.07

66.5
6.26
4.08

140–142


108
60.33
5.06
7.82

60.32
4.99
7.78

150–151


109
57.41
6.42
4.46

57.07
6.44
4.39

121–123


110
44.46
4.97
3.46





133–135


111
69.28
7.03
4.25

68.86
7.07
4.11

147–149


112
55.55
6.22
8.64

55.27
5.99
8.5

120–121


113
53.99
4.26
3.7

53.98
4.25
3.63

210











decom


114
57.49
7.39
4.74

57.72
7.57
4.43

80–83


115
65.5
7.9
4.77

64.97
7.79
4.75

90–92


116
65.5
7.9
4.77

65.11
8.03
4.71

125–127


117
71.26
8.3
4.2

70.6
7.89
4.83

94–96


118
56.29
4.17
7.72

56.23
4.01
7.6

173–175


119
47.89
3.81
3.29

47.52
3.71
3.16

236–237


120
55.7
6.55
13

55.71
6.58
13.05

123–5 


121
57.98
5.81
7.95

57.9
7.11
7.82

131–133


122
51.74
5.5
4.02

51.41
5.43
3.61

118–











119.5


123
41.22
4.38
3.2

41.45
4.36
2.94

143–











144.5


124
57.06
6.06
4.44

57.02
6.12
4.35

57–58


125
61.18
4.83
4.2

60.71
4.76
3.89

214











decom


126
55.55
6.22
8.64

55.4
6.24
8.53

150–151


127
65.17
4.83
4.47

65.27
4.87
4.48

208–209


128
73.03
8.99
4.06

72.92
9.36
4.1

 99–101


129
72.25
5.44
4

72.14
5.24
4.01

216–217


130
52.56
5.58
8.17

52.66
5.44
8.21

 96–100


131
56.28
6.41
9.38

56.32
6.42
9.28

 98–100


132
52.56
5.58
8.17

52.46
5.65
7.86

150–153


133
69.89
4.89
4.53

69.64
5
4.54

136–9 


134
71.68
5.2
4.2

71.24
5.1
4.13

251–253


135
65.64
5.78
4.25

65.3
5.91
4.04

79–83


136
33.92
3.61
2.64

34.48
3.84
2.48

164–165


137
57.06
6.06
4.44

57.09
6.17
4.45

88–89


138
69.79
7.69
5.09

69.68
7.78
5.08

102–3 


139
69.28
7.04
4.25

68.99
7
4.1

107–108


140
66.42
6.62
4.84

66.2
6.49
4.81

88–9 


141
58.62
6.76
4.27

58.66
6.93
4.18

134–135


142
63.38
7.21
5.28

63.22
7.28
5.24

71–73


143
56.29
4.17
7.72

56.19
4.04
7.65

156–160


144
71.13
7.88
3.77

70.39
7.91
3.64

95–97


145
58.44
6.06
8.02

58.25
6.38
7.84

165–8 


146
54.22
5.79
5.75

54.26
5.65
5.69

  77–78.5


147
54.22
5.79
5.75

54.21
5.85
5.61

80–81


148
58.78
4.93
40.3

58.64
4.89
3.97

172–173


149
56.19
4.72
3.85

56.31
4.67
3.86

177


150
66.46
4.65
4.31

66.41
4.56
4.23

158–160


151
58.61
7.24
5.69

58.79
7.35
5.66


152
54.22
5.79
5.75

54.21
5.72
5.62

54–55


153
60.85
4.25
7.89

60.27
4.37
7.89

>260


154
62.5
7.3
10.14

64.77
7.27
9.9

187–190


155
55.4
6.5
3.6

55.56
6.51
3.5

114–116


156
45.85
4.9
4.86

46.06
4.78
4.71

67–68


156
48.8
4.7
4.4

48.81
4.64
4.39

144–146


157
50.3
5.1
4.2

50.25
5.12
3.99

141–143


158
55.5
4.1
3.8

55.55
3.88
3.75

190–192


159
64.97
6.9
5.05

64.7
6.82
5.02

171–174


160
54.3
3.7
4

54.31
3.58
3.83

222–224


161
56.4
6.7
3.5

56.69
6.98
3.11

76–78


162
63.63
6.47
5.3

64.76
6.84
4.74

188–191


163
48.91
4.48
5.19

48.89
4.31
5.10

88.5–90  


164
66.66
10.04
5.18

66.69
10.77
5.16

67.5–70.5


165
39.42
4.21
4.18

39.19
4.35
3.88

oil


166
53.05
5.19
5.16

53.06
5.03
4.86

151–152


167
65.53
7.85
4.78

65.4
7.84
4.57

85–89


168
68.99
6.11
4.47

68.62
5.87
4.49

162–6 


169
69.71
6.47
4.28

69.67
6.58
4.50

132.5–











135


170
61.21
7.53
9.52

61.21
7.68
9.46

134–135


171
62.14
7.44
4.53

61.96
7.52
4.57

101–104


172
58.63
6.71
6.22

58.15
6.83
6.04


173
52.96
3.26
4.12

52.96
3.28
4.02

225–227


174
57.42
6.42
4.46

57.3
6.38
4.39

119–120


175
68.99
6.11
4.47

68.84
6.08
4.51

131–4 


176
66.43
8.2
4.56

66.42
8.16
4.51

109–110


177
62.14
6.82
5.57

61.96
6.66
5.52

127–128


178
51.00
4.56
3.97

51.09
4.61
3.93


179
67.36
5.30
4.90

67.26
5.24
4.91

185–186


180
66.43
8.20
4.56

66.32
8.60
5.12

51.5–55  


181
69.92
6.79
8.58

67.02
6.93
8.20

81–84


182
66.46
8.14
4.56

66.43
8.34
4.47

82–84


183
62.13
4.89
22.64

62.05
4.88
22.45

271–272


184
68.16
7.32
6.36

67.73
7.44
6.70

114–117


185
71.30
5.98
5.73

71.10
5.97
5.74

146–149


186
68.16
7.32
6.36

67.94
7.31
6.41

105–108


187
65.51
7.90
4.77

65.35
7.63
4.59

102–103


188
64.50
7.58
5.01

64.19
7.69
4.83

133–134


189
64.5
7.58
5.01

64.5
7.57
4.90

116–118


190
61.15
7.71
3.97

61.27
7.79
4.08

124–127


191
65.5
7.9
4.77

65.32
7.94
4.7

114–115


192
56.77
6.51
8.28

56.83
6.76
8.21

141–143


193
60.29
4.74
8.79

60.17
4.58
8.74

202–205


194
48.8
4.7
4.4

48.81
4.64
4.39

144–146









These carrier compounds or poly amino acids, and peptides, including the amino acids, may be used to deliver active agents including, but not limited to, biologically or chemically active agents such as for example, pharmacological and therapeutic agents.
An amino acid is any carboxylic acid having at least one free amine group and includes naturally occurring and synthetic amino acids.
Poly amino acids are either peptides or two or more amino acids linked by a bond formed by other groups which can be linked, e.g. an ester, anhydride, or an anhydride linkage.
Peptides are two or more amino acids joined by a peptide bond. Peptides can vary in length from dipeptides with two amino acids to poly peptides with several hundred amino acids. See Chambers Biological Dictionary, editor Peter M. B. Walker, Cambridge, England: Chambers Cambridge, 1989, page 215. Special mention is made of di-peptides, tri-peptides, tetra-peptides, and penta-peptides.
Salts such as, for example, sodium salt of these carrier compounds can be used as well.
Many of the compounds described herein are derived from amino acids.
Many of the compounds of the present invention can be readily prepared from amino acids including, but not limited to, aminocaprylic acid, butyrylhydroxaminic acid, aminophenylbutyric acid, aminophenylhexanoic acid, aminophenylpropionic acid, amino salicylic acid, aminophenylsuccinic acid, aminononanic acid, aminonicotinic acid, amino valenic acid, aminophenylacetic acid, aminocaproic acid, aminoundecanoic acid, aminoheptanoic acid, aminohydroxybenzoic acid, and aminodecanoic acid by methods within the skill of those in the art based upon the present disclosure and the methods described in U.S. patent application Ser. No. 60/017,902, filed Mar. 29, 1996; Ser. No. 08/414,654, filed Mar. 31, 1995; Ser. No. 08/335,148, filed Oct. 25, 1994; and 60/003,111, filed Sep. 1, 1995.
For example, these compounds may be prepared by reacting the single acid with the appropriate agent which reacts with free amino moiety present in the amino acids to form amides. Protecting groups may be used to avoid unwanted side reactions as would be known to those skilled in the art.
The carrier compound may be purified by recrystallization or by fractionation on solid column supports. Suitable recrystallization solvent systems include acetonitrile, methanol and tetrahydrofuran. Fractionation may be performed on a suitable solid column supports such as alumina, using methanol/n-propanol mixtures as the mobile phase; reverse phase column supports using trifluoroacetic acid/acetonitrile mixtures as the mobile phase; and ion exchange chromatography using water as the mobile phase. When anion exchange chromatography is performed, preferably a subsequent 0–500 mM sodium chloride gradient is employed.
Delivery Systems
The compositions of the present invention may include one or more active agents.
In one embodiment, compounds or salts of compounds 1–193 or poly amino acids or peptides that include at least one of these compounds or salts may be used directly as a delivery carrier by simply mixing one or more compound or salt, poly amino acid or peptide with the active agent prior to administration.
The administration mixtures are prepared by mixing an aqueous solution of the carrier with an aqueous solution of the active ingredient, just prior to administration. Alternatively, the carrier and the biologically or chemically active ingredient can be admixed during the manufacturing process. The solutions may optionally contain additives such as phosphate buffer salts, citric acid, acetic acid, gelatin, and gum acacia.
Stabilizing additives may be incorporated into the carrier solution. With some drugs, the presence of such additives promotes the stability and dispersibility of the agent in solution.
The stabilizing additives may be employed at a concentration ranging between about 0.1 and 5% (W/V), preferably about 0.5% (W/V). Suitable, but non-limiting, examples of stabilizing additives include gum acacia, gelatin, methyl cellulose, polyethylene glycol, carboxylic acids and salts thereof, and polylysine. The preferred stabilizing additives are gum acacia, gelatin and methyl cellulose.
The amount of active agent is an amount effective to accomplish the purpose of the particular active agent. The amount in the composition typically is a pharmacologically, biologically, therapeutically, or chemically effective amount. However, the amount can be less than a pharmacologically, biologically, therapeutically, or chemically effective amount when the composition is used in a dosage unit form, such as a capsule, a tablet or a liquid, because the dosage unit form may contain a multiplicity of carrier/biologically or chemically active agent compositions or may contain a divided pharmacologically, biologically, therapeutically, or chemically effective amount. The total effective amounts can then be administered in cumulative units containing, in total, pharmacologically, biologically, therapeutically or chemically active amounts of biologically or pharmacologically active agent.
The total amount of active agent, and particularly biologically or chemically active agent, to be used can be determined by those skilled in the art. However, it has surprisingly been found that with some biologically or chemically active agents, the use of the presently disclosed carriers provides extremely efficient delivery, particularly in oral, intranasal, sublingual, intraduodenal, or subcutaneous systems. Therefore, lower amounts of biologically or chemically active agent than those used in prior dosage unit forms or delivery systems can be administered to the subject, while still achieving the same blood levels and therapeutic effects.
The amount of carrier in the present composition is a delivery effective amount and can be determined for any particular carrier or biologically or chemically active agent by methods known to those skilled in the art.
Dosage unit forms can also include any of excipients; diluents; disintegrants; lubricants; plasticizers; colorants; and dosing vehicles, including, but not limited to water, 1,2-propane diol, ethanol, olive oil, or any combination thereof.
Administration of the present compositions or dosage unit forms preferably is oral or by intraduodenal injection.
The delivery compositions of the present invention may also include one or more enzyme inhibitors. Such enzyme inhibitors include, but are not limited to, compounds such as actinonin or epiactinonin and derivatives thereof. These compounds have the formulas below:




Derivatives of these compounds are disclosed in U.S. Pat. No. 5,206,384. Actinonin derivatives have the formula:





wherein R5 is sulfoxymethyl or carboxyl or a substituted carboxy group selected from carboxamide, hydroxyaminocarbonyl and alkoxycarbonyl groups; and R6 is hydroxyl, alkoxy, hydroxyamino or sulfoxyamino group. Other enzyme inhibitors include, but are not limited to, aprotinin (Trasylol) and Bowman-Birk inhibitor.

The compounds and compositions of the subject invention are useful for administering biologically or chemically active agents to any animals such as birds; mammals, such as primates and particularly humans; and insects. The system is particularly advantageous for delivering chemically or biologically or chemically active agents which would otherwise be destroyed or rendered less effective by conditions encountered before the active agent its target zone (i.e. the area in which the active agent of the delivery composition are to be released) and within the body of the animal to which they are administered. Particularly, the compounds and compositions of the present invention are useful in orally administering active agents, especially those which are not ordinarily orally deliverable.
DESCRIPTION OF THE PREFERRED EMBODIMENTS
The following examples illustrate the invention without limitation. All parts are given by weight unless otherwise indicated.
EXAMPLE 1
Carrier Preparation
General Preparations of Carriers. The following procedures were used to prepare the compounds described herein. Many of the compounds were prepared by reaction of the appropriate amino acid with the appropriate acid chloride. The preparation of compound 79 is given as a representative example of the compounds prepared in this manner.
Preparation of Compound 79. Method A. A 1 L round bottom flask fitted with a magnetic stirrer was charged with 3-(4-aminophenyl)propionic acid (46.3 g, 0.28 moles, 1.17 equiv.) and 2 M aqueous sodium hydroxide (300 mL). 2,3-dimethoxybenzoylchloride (48.0 g, 0.24 moles, 1.00 equiv.) was added portionwise over 1 h to the stirred solution. After the addition, the reaction was stirred for 2.5 h at ambient temperature, and the pH of the solution was kept at ca 10 by the addition of 10 M sodium hydroxide. The solution was then acidified with 1 M hydrochloric acid (3×100 mL), water (100 mL), and air dried. It was redissolved in boiling acetone (ca 500 mL), decolorized with activated charcoal (3 g), and filtered. Water (1.5 L) was added to the filtrate to induce the formation of a brown oil. The brown oil solidified upon stirring at room temperature for 10 min. The crude solid was collected by filtration and recrystallized from 70% methanol-water (v/v) to afford compound 79 as a tan solid (39.5) g, 50%).
Compounds 1, 5, 30, 31, 33, 36, 53–66, 68, 69, 71–74, 78, 80–88, 95, 97–99, 102, 108–110, 112–115, 119, 121–126, 136, 137, 139, 141, 144, 146, 147, 151, 152, 155–158, 160, 161, 163, 165, 166, 170, 172–174, 176, 177, 184–186, 188, 189, 191 and 192 were also prepared by this process.
Preparation of Compound 79. Method B. A 2 L three-neck round bottom flask was fitted with a magnetic stirrer and two addition funnels under an argon atmosphere. A suspension of 3-(4-aminophenyl)propionic acid (46.3 g, 0.28 moles, 1.17 equiv.) in ethyl acetate (700 mL) was added to the flask. A solution of 2,3-dimethoxybenzoylchloride (48.0 g, 0.24 moles, 1.00 equiv.) in ethyl acetate (250 mL) was charged to one of the addition funnels and added dropwise over 1 h. Triethylamine (28.20 g, 0.28 moles, 1.00 equiv.) was subsequently charged to the second funnel and added dropwise over 15 min. The reaction was stirred at ambient temperature for 3 h, and the solvent was evaporated in vacuo giving a residual brown oil. Water (600 mL) was added to the residue followed by sodium hydroxide (2 M, 500 mL), and the mixture was stirred at ambient temperature for 3 hours. The resultant brown solution was acidified with 2 M hydrochloric acid (ca 1 L). After cooling the mixture in an ice bath for 1 h, a yellow solid formed and was collected by filtration. The solid was washed with water (3×1.5 L) and recrystallized from 50% ethanol-water (v/v) to give compound 79 as a tan solid (59.2 g, 68%).
Compounds 18, 32, 37, 41, 168, 175, and 183 were also prepared by this process.
Preparation of Compound 79. Method C. A 2 L round bottom flask equipped with a magnetic stirrer and a reflux condenser was charged with a suspension of 3-(4-aminophenyl)propionic acid (46.3 g, 0.28 moles, 1.17 equiv.) in dichloromethane (560 mL). Chlorotrimethylsilane (62.36 g, 0.57 moles, 2.05 equiv.) was added in one portion, and the mixture was heated to reflux for 1 h under argon. The reaction was allowed to cool to room temperature and was placed in an ice bath (internal temperature <10° C.). The reflux condenser was replaced with an addition funnel containing triethylamine (42.50 g, 0.42 moles, 1.50 equiv.). The triethylamine was added dropwise over 15 min, and a yellow solid formed during the addition. The funnel was replaced by another addition funnel containing a solution of 2,3-dimethoxybenzoylchloride (48.0 g, 0.24 moles, 1.00 equiv. in dichloromethane (100 mL). The solution was added dropwise over 30 min. The reaction was stirred in the ice bath for another 30 min and at ambient temperature for 1 h. The dichloromethane was evaporated in vacuo to give a brown oil. The brown oil was cooled in an ice bath, and an ice-cold solution of 2 M sodium hydroxide (700 mL) was added. The ice bath was removed, and the reaction was stirred for 2 h to afford a clear brown solution. The solution was acidified with 2 M sulfuric acid (400 mL) and stored at ca 5° C. for 1 hour. A yellow solid formed and was collected by filtration. The solid was washed with water (3×100 mL) and recrystallized from 50% ethanol-water (v/v) to afford compound 79 as tan needles (64.7 g, 82%).
Compounds 2–4, 6–17, 19–29, 34, 38–40, 42–48, 50–52, 67, 70, 75–77, 89–94, 96, 100, 101, 107, 111, 116–118, 127–132, 134, 135, 193, 142, 143, 148, 149, 159, 162, 164, 169, 178–182, 187, and 190 were also prepared by this process.
Preparation of Compound 35. A solution of O-acetylsalicyloyl chloride (24.68 g, 124 mmol, 1 equiv) in tetrahydrofuran (300 mL) was cooled in an ice bath. Triethylamine (25 g, 249 mmol, 2 equiv) was added dropwise via an additional funnel. The methyl 9-aminononanoate hydrochloride was dissolved in DMF (190 mL, slightly warm to dissolve), charged to an addition funnel and added dropwise to the above mixture. The reaction was stirred in the ice-bath for 20 min and at room temperature for 2 h. Evaporation of the THF under reduced pressure gave a pink DMF solution. The pink solution was cooled in an ice-bath, and 2 M aqueous sodium hydroxide (300 mL) was added. After being stirred at room temperature for 12 h, the mixture was acidified with 2 M hydrochloric acid (500 mL). The solution was cooled in an ice-bath, and a solid formed. The solid was collected by filtration and was recrystallized from 50% ethanol/water to give compound 35 (32 g, 87%) as an off-white solid.
Preparation of Compound 49. 1-(2-hydroxyphenyl)-3-(4-methyl benzoate)-1,3-propane dione (3.00 g, 0.0101 mil.) is placed in a 100 ml round bottomed flask fitted with argon purge, magnetic stir bar and cold water condenser. Glacial acetic acid (20 mls) and concentrated sulfuric acid (5 mls) were added, and heating of the reaction mixture was initiated. The reaction mixture was allowed to heat at reflux for 6 h before heating was discontinued. The reaction mixture was allowed to come to room temperature, and then was poured into 100 mls of ice/water. This was stirred for approximately 1⁄2 h before the mixture was filtered, and a brown solid was isolated. The brown solid was recrystallized twice from acetic acid, yielding compound 49 as a tan solid (1.44 g, 53.8%).
Preparation of Compound 167. 2-coumaranone (4.21 g, 0.0314 mol) was dissolved, with stirring, in acetonitrile (75 mls) in a 250 ml round bottomed flask fitted with a magnetic stir bar, argon purge and cold water condenser. Triethylamine (3.18 g, 0.0314 mol) and 8-aminocaprylic acid (5.00 g, 0.0314 mol) were added, and a tan slurry was formed. Heating was started, and the reaction mixture was allowed to reflux overnight. After heating overnight, thin layer chromatography of the reaction mixture (50% ethyl acetate/50% hexane) indicated that the reaction had gone to completion. Heating was stopped, the reaction mixture was allowed to cool to room temperature, and was concentrated in vacuo. The resulting residue was taken up in methylene chloride, and was washed with two, 100 ml portions of 1N hydrochloric acid solution. The methylene chloride layer was dried with sodium sulfate and was concentrated in vacuo. The resulting tan solid was allowed to dry in vacuo overnight, yielding compound 167 as a tan solid (8.35 g, 70.4%).
Preparation of Compound 171. 1,4-benzodioxan-2-one (3.93 g, 0.0262 mol) was dissolved, with stirring, in acetonitrile (70 mis) in a 250 ml round bottomed flask fitted with a magnetic stir bar, argon purge and cold water condenser. Triethylamine (2.64 g, 0.0262 mol) and 8-aminocaprylic acid (500 g, 0.0262 mol) were added and a tan slurry was formed. Heating was started, and the reaction mixture was allowed to reflux for approximately 3 hours. At this time, thin layer chromatography of the reaction mixture (50% ethyl acetate/50% hexane) indicated that the reaction had gone to completion. Heating was discontinued, and the reaction mixture was allowed to cool to room temperature and was concentrated in vacuo. The resulting residue was taken up in methylene chloride and was washed with a 100 ml portion of 1 N hydrochloric acid solution. At this time, a tan solid was noted to precipitate, and it was isolated by filtration. This tan solid was washed further with an additional 100 ml portion of 1 N hydrochloric acid solution, and then with 100 ml of water. The resulting tan solid was allowed to dry in vacuo overnight yielding Compound 171 as a tan solid (7.73 g, 95.6%).
Preparation of Compound 120. A solution of 3.00 g (18.3 mmol) of 2-nitrophenylisocyanate and 5 mL of tetrahydrofuran was dropwise over 10 min to an ice bath-cooled solution of 2.08 g (13.1 mmol) of 8-aminocaprylic acid, 1.40 mL of 10 N NaOH and 40 mL of water. The reaction mixture was stirred an additional 30 min, warmed to 25° C. and treated with 3% HCl solution until the pH was 5. The yellow precipitate was filtered off and rinsed with 100 ml of water. The yellow solid was recrystallized in 2-propanol and water to give 3.7 g of compound 120 as pale yellow crystals.
Compounds 104–106 were also prepared by this procedure.
Preparation of Compound 133. A suspension of 2.40 g (16.3 mmol) and 2.80 g (15.6 mmol) of 4-(4aminophenyl)butyric acid in 20 mL of propylene glycol, 2.40 mL (1.74 g, 17.3 mmol) of triethylamine and 10 mg (0.08 mmol) of dimethylaminopyridine was heated to 140° C. The mixture became a clear solution after 5 min at 140° C. After stirring for 330 min, the reaction mixture was cooled to 25° C. and diluted with 20 mL of water. The solid phthalimide which had formed was filtered off. The filtrate was acidified with 3% HCl solution. The resulting solid was filtered off and was recrystallized from 2-propanol and water to give 0.62 g of compound 133 as a tan solid.
Preparation of Compound 138. A solution of 1.73 g (12.9 mmol). of phthalic dialdehyde, 2.04 g 8-aminocaprylic acid and 20 mL of acetic acid was heated to reflux for 10 min. The reaction mixture was cooled to 40° C., diluted with water and extracted with CH2Cl2 (2×20 mL). The organic phase was washed with water and brine, dried over Na2SO4 and evaporated. The residue was dissolved in ether and extracted with 2N NaOH. The layers were separated. The aqueous layer was made acidic with 3% HCl and extracted with CH2Cl2. The organic phase was dried over Na2SO4 and evaporated. The yellow residue was crystallized from acetonitrile and water to give 1.25 g of compound 138 as a yellow solid.
Preparation of Compound 140. A mixture of 1.40 g (9.48 mmol) of phthalic anhydride and 1.51 g (9.48 mmol) of 8-aminocaprylic acid was heated to 150° C. for 5 min. Upon cooling, 2.61 g of solid compound 140 was received.
Compound 150 was also prepared by this procedure.
Preparation of Compound 145. A suspension of 2.11 g (10.1 mmol) ethyl carbamoylanthranilic acid and 5 mL of CH2Cl2 was treated with 2.20 mL of oxalyl chloride. After stirring for 1 h the volatiles were stripped off. At that same time, a suspension of 1.60 g (10.1 mmol) of 8-aminocaprylic acid and 15 mL of CH2Cl2 was treated with 2.60 mL (2.23 g, 20.5 mmol) of TMSCl. This mixture was heated to reflux for 90 min, cooled in an ice bath and treated with 4.30 mL (3.12 g, 30.9 mmol) of triethylamine. Five min later, a slurry of the residue from the oxalyl chloride reaction in 20 mL of CH2Cl2 was added. The reaction mixture was warmed to 25° C. and stirred overnight. Upon acidification of the mixture with 3% HCl, a white solid formed. The solid was filtered off and recrystallized from EtOH and water to give 1.88 g of compound 145.
Compound 153 was also prepared by this procedure.
Preparation of Compound 154. A suspension of 4.02 g (25.6 mmol) of trans-4-aminomethylcyclohexane-carboxylicacid, 4.18 g (25.6 mmol) of isatoic anhydride, 20 mL of CH2Cl2, 20 mL of dioxane, and 4 mL of water was heated to reflux for 12 h. The solution was cooled to 25° C. and extracted with ether (4×20 mL). The organic layer was dried over Na2SO4 and concentrated. The resulting solid was recrystallized from EtOH and water to give 4.95 g of compound 154.
Compound 103 is available from Aldrich Chemical Company, Inc., Milwaukee, Wis.
EXAMPLE 2
Parathyroid Hormone Dosing Solutions
Intracolonic (“IC”) dosing compositions containing 100 mg/kg of carrier and 25 μg/kg of parathyroid hormone in 25% aqueous propylene glycol or oral gavage “PO”) dosing solution containing 400 mg/kg of carrier and 100 μg/kg of parathyroid hormone in water, were prepared with carriers 9, 33, 35, 77, 79, 109, 110, 123, 136, 141, and 169. The dosing solutions are designated P-carrier number-DS.
COMPARATIVE EXAMPLE 2A
Parathyroid Hormone Dosing Solutions
An intracolonic dosing composition containing 100 mg/kg of a carrier having the formula




and 25 ug/kg of parathyroid hormone in 25% aqueous propylene glycol was prepared. The dosing solution is identified as P-9A-DS.

EXAMPLE 3
In vivo Parathyroid Hormone Delivery
Male Sprague-Dawley rats weighing between 200–250 g were fasted for 24 hours and were administered ketamine (44 mg/kg) and chlorpromazine (1.5 mg/kg) 15 minutes prior to dosing. The rats were administered one of dosing solutions P-9-DS, P-33-DS, P-35-DS, P-77-DS, P-79-DS, and P-141-DS by oral gavage (“PO”) or intra-colonic instillation (“IC”). Blood samples were collected serially from the tail artery for serum determination of parathyroid hormone concentration. Serum parathyroid hormone concentrations were quantified by a parathyroid hormone immunoaccuracy test host.
Results are illustrated in Table 2, below.
COMPARATIVE EXAMPLE 3A
In vivo Parathyroid Hormone Delivery
The procedure of Example 3 was followed substituting dosing solution P-9A-DS for dosing solution P-9-DS. Results are illustrated in Table 2, below.
COMPARATIVE EXAMPLE 3B
In vivo Parathyroid Hormone Delivery
The procedure of Example 3 was followed with a dosing solution (at a dose of 25 μg/kg of parathyroid hormone (intra-colonic) or 100 μg/kg of parathyroid hormone (oral)), P-ØA-DS, that omitted the carrier.
Results are illustrated in Table 2, below.







TABLE 2







In vivo Parathyroid Hormone Delivery











Mean Peak Serum [PTH] ±



Dosing Solution
Standard Deviation (pg/ml)







P-9-DS
155 ± 105 (IC)



P-33-DS
58 ± 18 (IC)



P-35-DS
50 ± 27 (IC)



P-77-DS
358 ± 274 (PO)



P-79-DS
521 ± 128 (PO)



P-109-DS
128 ± 25 (IC)



P-110-DS
35 ± 11 (IC)



P-123-DS
49 ± 22 (IC)



P-136-DS
106 ± 72 (IC) 



P-141-DS
120 ± 120 (PO)



P-169-DS
19 ± 33 (IC)



P-9A-DS
116 ± 48 (IC) 



P-ØA-DS
11 ± 2 (PO) ,




27 ± 27 (IC)










EXAMPLE 4
Recombinant Human Growth Hormone Dosing Solutions
Intracolonic dosing compositions containing 25 mg/kg of carrier and 1 mg/kg of rHGH in phosphate buffer or oral gavage dosing solutions containing 600 mg/kg of carrier and 3 mg/kg of rHGH in phosphate buffer were prepared with carriers 9, 35, 36, 47, 62, 64, 67, 77, 79, 90, 94, 107, 109, 136, and 141.
The dosing solutions are designated R-carrier number-DS.
COMPARATIVE EXAMPLE 4A
Recombinant Human Growth Hormone Dosing Solutions
An intracolonic dosing solution was prepared according to the procedure of Example 4, substituting a carrier having the formula




for the carrier. This dosing solution is designated as R-35A-DS.

COMPARATIVE EXAMPLE 4B
Recombinant Human Growth Hormone Dosing Solutions
An intracolonic dosing solution was prepared according to the procedure of Example 4, substituting a carrier having the formula




for the carrier. This dosing solution is designated as R-35B-DS.

COMPARATIVE EXAMPLE 4C
Recombinant Human Growth Hormone Dosing Solutions
An intracolonic dosing solution was prepared according to the procedure of Example 4, substituting a carrier having the formula




for the carrier. This dosing solution is designated as R-9A-DS.

EXAMPLE 5
In Vivo Recombinant Human Growth Hormone Delivery
Male Sprague-Dawley rats weighing 200–250 g were fasted for 24 hours and administered ketamine (44 mg/kg) and chlorpromazine (1.5 mg/kg) 15 minutes prior to dosing. The rats were administered one of the dosing solutions of Example 3 by either oral gavage or intracolonic instillation. Blood samples were collected serially from the tail artery for determination of serum rHGH concentrations. Serum rHGH concentrations were quantified by an rHGH immunoassay test kit.
Results are illustrated in Table 3, below.
COMPARATIVE EXAMPLE 5A
In Vivo Recombinant Human Growth Hormone Delivery
The procedure of Example 5 was followed, substituting the dosing solutions of Comparative Examples 3A–3C for the dosing solutions. Results are illustrated in Table 3, below.
COMPARATIVE EXAMPLE 5B
In Vivo Recombinant Human Growth Hormone Delivery
The procedure of Example 5 was followed, with dosing solutions of active agent (at a dose of 1 mg of rHGH/kg (intracolonic) or 3 mg of rHGH/kg (oral) and no carrier. These dosing solutions are designated R-ØD-DS and R-ØE-DS, respectively. Results are illustrated in Table 3, below.







TABLE 3







In Vivo Recombinant Human Growth Hormone Delivery











Mean Peak Serum [rHGH] ±



Dosing Solution
Standard Deviation (ng/ml)














R-9-DS
125 ± 34 (IC) 



R-35-DS
41 ± 46 (PO)




108 ± 56 (IC) 



R-36-DS
28 ± 11 (IC)



R-47-DS
0 (IC)



R-62-DS
11 ± 12 (IC)



R-64-DS
72 ± 22 (PO)



R-67-DS
19 ± 22 (PO)




88 ± 24 (IC)



R-77-DS
34 ± 10 (PO)



R-79-DS
62 ± 51 (PO)



R-90-DS
 9 ± 13 (PO)



R-94-DS
39 ± 35 (PO)



R-107-DS
0 ± 0 (PO)



R-109-DS
128 ± 25 (IC) 



R-136-DS
106 ± 72 (IC) 



R-141-DS
95 ± 14 (IC)



R-35A-DS
17 ± 3 (IC) 



R-35B-DS
42 ± 28 (IC)



R-9A-DS
55 ± 17 (IC)



R-ØD-DS
0 ± 0 (IC)



R-ØE-DS
0 ± 0 (IC)










EXAMPLE 6
In Vivo Interferon Delivery
An intracolonic dosing composition containing 50 mg/kg of carrier 9 and 250 μg/kg of interferon in 50% propylene glycol was prepared. Rats were administered the dosing composition by intracolonic instillation. Delivery was evaluated by use of an ELISA assay for human interferon a from Biosource, Inc. Mean peak serum interferon concentration was 2611±695.
COMPARATIVE EXAMPLE 6A
In Vivo Interferon Delivery
Rats were administered, orally and by intracolonic instillation, dosing solutions of 1 mg/kg of interferon and no carrier. Delivery was evaluated according to the procedure of Example 6. Mean peak serum interferon concentration was 1951±1857 (PO) and 79±100 (IC).
EXAMPLE 7
Heparin Dosing Solutions
Intracolonic dosing compositions containing 50 mg/kg of carrier and 25 mg/kg of heparin in 25% aqueous propylene glycol or oral gavage dosing solutions containing 300 mg/kg of carrier and 100 mg/kg of heparin in 25% aqueous propylene glycol were prepared with carriers 9, 35, 47, 50, 58, 62, 64, 67, 76, 96, 102, 109, 110, 111, 117, 122, 123, 139, 141, 144, and 169. The dosing solutions are designated H-carrier number-DS.
COMPARATIVE EXAMPLE 7A
Heparin Dosing Solutions
Comparative intracolonic dosing compositions were prepared according to the procedure of Example 7, substituting the following carriers for the carrier.




These dosing solutions are designated H-35A-DS, H-35B-DS, and H-109A-DS, respectively.
EXAMPLE 8
In Vivo Evaluation of Heparin in Rats
The dosing solutions of Example 7 were administered to fasted rats either by oral gavage or intracolonic instillation.
Blood samples were collected by cardiac puncture following the administration of ketamine (44 mg/kg). Heparin activity was determined by utilizing the activated partial thromboplastin time (APTT) according to the method of Henry, J. B., Clinical Diagnosis and Management by Laboratory Methods; Philadelphia, Pa.; W. B. Saunders (1979).
Results are in illustrated in Table 4, below.
COMPARATIVE EXAMPLE 8A
In Vivo Evaluation of Heparin in Rats
The dosing solutions of Comparative Example 7A were administered to fasted rats by intracolonic instillation. Blood samples were collected and heparin activity was determined by the method of Example 8.
Results are illustrated in Table 4, below.
COMPARATIVE EXAMPLE 8B
In Vivo Evaluation of Heparin in Rats
An intracolonic dosing solution of 25 mg/kg of heparin and an oral gavage dosing solution of 100 mg/kg of heparin were administered to fasted rats. These dosage solutions were designated H-ØA-DS and H-ØB-DS, respectively.
Blood samples were collected, and heparin activity was determined by the methods of Example 8.
Results are illustrated in Table 4, below.







TABLE 4







In Vivo Evaluation of Heparin in Rats










Dosing Solution
Heparin APTT (sec)







H-9-DS
48 ± 18 (IC)



H-35-DS
54 ± 27 (PO),




177 ± 85 (IC) 



H-47-DS
30 ± 14 (IC)



H-50-DS
40 ± 22 (IC)



H-58-DS
24 ± 4 (IC) 



H-62-DS
37 ± 13 (IC)



H-64-DS
59 ± 28 (PO),




168 ± 75 (IC) 



H-67-DS
76 ± 36 (IC)



H-76-DS
63 ± 27 (PO)



H-96-DS
36 ± 8 (IC) 



H-102-DS
111 ± 108 (IC)



H-109-DS
56 ± 28 (IC)



H-110-DS
37 ± 9 (IC) 



H-111-DS
71 ± 39 (IC)



H-117-DS
140 ± 128 (IC)



H-122-DS
49 ± 21 (IC)




207 ± 7 (PO) 



H-123-DS
42 ± 14 (PO)



H-139-DS
31 ± 11 (PO)



H-141-DS
59 ± 26 (IC)



H-144-DS
26 ± 3 (IC) 



H-35A-DS
61 ± 29 (IC)



H-35B-DS
51 ± 30 (IC)



H-169-DS
23 ± 2 (IC) 



H-ØA-DS
23 ± 2 (PO) 



H-ØB-DS
33 ± 6 (IC) 










The above mentioned patents, applications, test methods, and publications are hereby incorporated by reference in their entirety.
Many variations of the present invention will suggest themselves to those skilled in the art in light of the above detailed description. All such obvious variations are within the full intended scope of the appended claims.

## Claims

1. A compound comprising of:



or a salt thereof.