# Patent

## Title
Generation of binding molecules

## Abstract

Provided are methods for efficiently and comprehensively screening antibody repertoires from B cells to obtain and produce molecules with binding characteristics and functional activities for use in human therapy.

## Background
CROSS REFERENCE TO RELATED APPLICATIONS
This application is a continuation of U.S. Ser. No. 13/627,381 filed Sep. 26, 2012, now allowed, which claims priority to U.S. Provisional Application No. 61/539,116, entitled “Generation of Binding Molecules”, filed Sep. 26, 2011, the entire contents of which are incorporated herein by this reference.


SUBMISSION OF SEQUENCE LISTING ON ASCII TEXT FILE
The content of the following submission on ASCII text file is incorporated herein by reference in its entirety: a computer readable form (CRF) of the Sequence Listing (file name: 737572000201SeqList.txt, date recorded: Jan. 4, 2016, size: 30,726 bytes).
BACKGROUND OF THE INVENTION
The ability of the mammalian immune response to generate a large and diverse antibody repertoire in response to antigen has been exploited for a range of applications in diagnostics, therapy and basic research. In particular, monoclonal antibodies, the products of a single B cell clone, have been broadly applied because of their well-defined specificity and ease of production. Typically, monoclonal antibodies or the genetic information encoding monoclonal antibodies with desirable specificities are obtained from the B cells of animals or humans that have been immunized with antigen or infected by pathogens. Alternatively, monoclonal antibodies may be obtained by one of several recombinant-DNA-based methods to construct and screen libraries of antibodies or antibody fragments expressed on the surface of bacteriophages or eukaryotic cells, or from in silica approaches.
Monoclonal antibodies have found increasing use in human therapy for the treatment of a variety of diseases, including for example chronic inflammatory diseases and cancer. The immunogenicity of xenogeneic monoclonal antibodies limits their use in the therapy of human disease. Exposure of patients to xenogeneic antibodies often results in adverse effects or might lead to the neutralization and clearance of the applied antibody, thereby reducing its pharmacological efficacy (Clark, 2000). Administration of humanized or fully human monoclonal antibodies to patients usually diminishes the aforementioned complications, in particular when the amino acid sequences of the antibodies do not contain epitopes that stimulate T cells. Antibodies encoded by non-mutated, human germline heavy and light chain V gene segments containing CDR3 regions devoid of T cell epitopes represent ultimate examples of protein drugs with low immunogenicity (Ruuls et al., 2008; Harding et al., 2010). So, for therapeutic applications, monoclonal antibodies are preferably fully human, non-mutated and contain few or no T cell epitopes to prevent the formation of anti-drug antibodies.
B cells from the blood or lymphoid organs of humans have been used as a source of therapeutic monoclonal antibodies. Since the discovery of the hybridoma technology for immortalization of murine B cells (Kohler et al., 1975) and the realization that this technology could not be readily replicated using human B cells, several alternative methods for the generation of human monoclonal antibodies have been developed. Such methods include transformation of B cells through Epstein-Barr virus infection (Tragiai et al., 2004), short term activation and expansion of human B cells by stimulation with combinations of stimulator cells, antibodies and cytokines (Zubler 1987/Banchereau et al., 1991/Kim et al., 2001/Good et al., 2006/Ettinger et al., 2005) or retrovirus-mediated gene transfer (Kwakkenbos et al., 2010), cloning of antibody V genes from single human B cells by PCR (Wrammert et al., 2010/Meijer et al., 2006), and identification and selection of antigen-specific antibody-secreting B cells by hemolytic plaque assays (Babcook et al., 1996). Human B cell immortalization or activation techniques are compatible with only some stages of B cell maturation and furthermore, due to their low efficiencies (merely 1-3% of B cells) they are not suitable for efficient interrogation of the whole repertoire of specific antibodies generated during a human immune response for antibodies with desired characteristics (Reddy et al., 2011).
Single-cell cloning, a procedure in which single human B cells are plated in microtiter well plates for analysis, has been used to circumvent the low efficiencies associated with procedures that require B cell activation and/or immortalization to obtain human monoclonal antibodies. In this approach, RNA from individual B cells is used to amplify the variable regions of the heavy and light chain (VH, VL) of antibodies by PCR. The VH and VL genes are then inserted into suitable expression vectors for transfection into cell lines and subsequent production of recombinant antibody fragments or full-length IgG (Smith et al., 2009/Tiller et al., 2008). Alternatively, amplified VH and VL genes may be directly used for in vitro transcription and translation to generate minute quantities of antibodies sufficient for binding analysis but nor for assessing functional activity (Jiang et. al., 2006). Using these procedures, the production of recombinant monoclonal antibodies is not limited to distinct B cell populations and does not depend on prior stimulation or immortalization. The major challenge in this approach is the specific amplification of antibody genes by RT-PCR from single cells and the occurrence of cross-contamination during handling of large numbers of PCR reactions. Another practical limitation is the number of individual B cells that can be handled, which is typically restricted to several thousand, preventing extensive sampling of the entire antibody repertoire generated during an immune response. Finally, the method is restricted to the analysis of readily accessible human B cells such as those derived from blood and bone marrow.
Human monoclonal antibodies can also be isolated from recombinant antibody libraries in the laboratory, using one of the platforms for selection that in essence mimics the in vivo antibody response (Hoogenboom, 2005). For example, display technologies exploit large collections of cloned antibody variable regions expressed on the surface of phage particles, bacteria, eukaryotic cells or ribosomes to select for antibodies that bind to antigens of interest (Ponsel et al., 2011/Clackson et al., 1991/Boder et al., 1997/Fuchs et al., 1991/Lee et al., 2007/Chao et al., 2006). The VH and VL regions inserted in these display systems are randomly combined to form collections of antibody binding sites, i.e. fragments of intact IgG antibodies, which require correct folding and assembly in e.g. prokaryotic cells for retrieval by antigen-binding methods. Display methods do not allow the retrieval of antibodies from libraries through functional screening. In display approaches, original pairing of heavy and light chains is abrogated and, in addition, antibody-encoding DNA is lost as a result of the use of restriction enzymes during the cloning procedure. The success of recovering desired antibody specificities with in vitro antibody discovery techniques depends not only on the successful folding and expression of the recombinant antibody fragments in e.g. prokaryotic cells but also on a range of screening parameters used during antibody selection. These include the nature of the display platform, antigen concentration, binding avidity during enrichment, the number of selection rounds, and the design and diversity of the antibody libraries (Hoogenboom 2005/Cobaugh et al., 2008 Persson et al., 2006). Thus, due to experimental procedures, folding requirements for expression of antibody fragments in prokaryotic cells and parameters affecting the success of antibody retrieval during selections, display systems do not permit the comprehensive mining of antibody repertoires and do not allow direct functional screening of human antibodies. Indeed, antigen-specific antibody fragments may be lost during subsequent rounds of antigen selection of phage display libraries (Ravn et al., 2010).
Transgenic mice harboring collections of human antibody genes have been constructed to alleviate some of the restrictions associated with the use of human B cells as starting material for the generation of human monoclonal antibodies (Lonberg 2005). Such mice can be immunized with any antigen and their lymphoid organs are readily accessible for harvesting B cells. Once the transgenic mouse has been immunized, monoclonals can either be obtained through traditional hybridoma generation, by display technologies or using approaches that involve the harvesting, plating and screening of B cells, followed by isolation of mAb genes and cloning into production cell lines.
For the generation of hybridomas, B cells from murine lymphoid organs are harvested and fused with myeloma cells to form immortalized monoclonal antibody-secreting cell lines. The low efficiency of cell fusion in hybridoma formation permits interrogation of only a fraction of the antibody repertoire and is restricted to B cell populations that are amenable to fusion. If a satisfactory hybridoma is not formed, it becomes difficult to obtain the antibody against challenging antigens such as membrane proteins. Thus, increasing the numbers of hybridomas is a crucially important step in screening the repertoire of antigen-specific B cells from immunized mice and obtaining monoclonal antibodies with high affinity, specificity and desired functional activity (Kato et al., 2011/Li et. al., 1994; Crowe, 2009). In the most efficient fusion protocols involving pre-stimulation of B cells and electrofusion, approximately 1 in 1000 B cells fuses successfully with a myeloma cell to become an antibody-secreting hybridoma (Kato et. al., 2011). The hybridoma technology and other B cell immortalization methods interrogate the antibody-producing cells in pre-plasma cell B cell populations, specifically in memory B cells, or in circulating short-lived plasma blasts (Wrainmert et al., 2008).
B cells from immunized transgenic mice with human antibody genes may be used to obtain collections of VH and VL regions that are randomly combined to form combinatorial display libraries of human antibody fragments. As argued above, due to experimental procedures, folding requirements for expression of antibody fragments in prokaryotic cells and parameters affecting the success of antibody retrieval during selections, display systems do not permit the comprehensive mining of antibody repertoires and do not allow direct functional screening of human monoclonal antibodies.
High-throughput sequencing has been utilized for sequencing of antibody repertoires derived from bone marrow plasma cells of protein-immunized mice (Reddy et al., 2010). It was found that in the purified plasma cell population, VH and VL repertoires were highly polarized with the most abundant sequences representing 1-10% of the entire repertoire (Reddy et al., 2010). The most abundant VH and VL genes were randomly-paired, expressed as IgG molecules and screened for binding to the immunizing antigen.
A disadvantage of random pairing is that only 4% of the thus generated antibodies were found to bind to the immunizing antigen. These antibodies had low affinities and/or poor expression levels and aggregation was frequently observed. The low proportion of specific antibodies could be improved by pairing VH and VL genes based on their relative frequency in the collection of sequences. In that case, following recombinant expression, approximately 75% of antibodies were found to bind to antigen (Reddy et al., 2010). The disadvantage of VH/VL pairing according to relative frequencies is that collections of V-genes obtained by high throughput sequencing may contain VH and VL sequences that are present in similar frequencies yet are derived from different B cell clones and thus may not represent a natural pair and may not form a functional antibody molecule. Pairing of VH and VL regions based on frequency is therefore inaccurate and may lead to the generation and screening of many antibodies that have mismatched VH/VL pairs encoding low affinity antibodies or antibodies that do not bind to the target of interest. Indeed, it has been shown that VH/VL pairing based on relative frequencies yields a high proportion of modest to low affinity antibodies (Reddy et al., 2010). This implies that VH/VL pairing based on high frequency of VH and VL genes present in large collections of sequences is not predictive for the generation of high affinity antibodies. Thus, such an approach yields only small numbers VH/VL combinations encoding antigen-specific antibodies which were generally found to have low affinities (Reddy et al., 2010).
A further disadvantage of the method reported by Reddy et al. is that it relied on plasma cells as a source of antigen-specific monoclonal antibodies. Plasma cells represent only a small subpopulation of B-lineage cells contributing to antibody diversity generated during an immune response. As a result antigen specific antibodies produced by other B cell populations during an immune response are not retrieved. These populations include short-lived plasma cells, transitional B cells, germinal center B cells and IgM and IgG memory B cells present in lymphoid organs. When comparing antibody repertoires in these various B cell populations, significant changes were observed (Wu, et al., 2010) which implies that a broader antibody repertoire is captured when more B cell populations are included as source for VH/VL in deep sequencing.
Based on the above, it can be concluded that there is a need for antibody generation and selection approaches that facilitate the interrogation of entire antibody repertoires for antibodies encoded by original VH/VL pairs with desirable binding characteristics and functional activities.
SUMMARY OF THE INVENTION
To recapitulate, for selection and screening of the entire repertoire of antibodies produced during an immune response it is necessary to exploit techniques that allow efficient retrieval of antigen-driven, clonally expanded B cells of various phenotypes and subpopulations and/or the genetic information encoding the corresponding antibodies as original VH/VL pairs. The present invention provides a method to efficiently and comprehensively interrogate the broad spectrum of antibodies generated by B cell populations. Preferably, these diverse B cell populations are obtained from transgenic animals, e.g. mice, which harbor human antibody genes to facilitate immunization with any desirable antigen and generate antibodies for therapeutic application in humans. It is most preferred that these transgenic mice have a limited VL repertoire, in particular a single human rearranged VL. The method is independent of B cell immortalization or activation procedures, facilitates screening of antibody repertoires from B cells obtained from essentially all lymphoid organs and does not require analyses of individual B cells. Preferably, to efficiently mine the entire repertoire of antibodies generated during an immune response, B cells from every differentiation stage and lineage and present in relevant lymphoid organs such as lymph nodes, spleen blood and bone marrow are analyzed for the presence of monoclonal antibodies of desired specificity and characteristics such as affinity and functional activity. The method may thus address the entire population of B cells in lymphoid organs or focus on B cells subpopulations that are distinguishable based on phenotypic characteristics such as transitional B cells, memory B cells, short-lived plasma cells and the like. Furthermore, the method allows direct screening of antibodies for binding characteristics as well as functional activity in the relevant antibody format that is representative for eventual application in human therapy. The invention further provides for methods and means for production of these selected antibodies in the desired formats as well as these antibodies and their uses themselves. These uses include arrays as well as pharmaceutical products.
The invention provides a method for producing a defined population of binding molecules, said method comprises at least the following steps: a) providing a population of B cells expressing a limited VL repertoire wherein essentially all of said B cells carry at least one VL, b) obtaining nucleic acids (RNA or DNA) from said B cells, c) optionally, amplification of nucleic acid sequences encoding immunoglobulin heavy chain variable regions in said sample, d) at least partial sequencing of all obtained nucleic acids of step b) or the amplification products of step c), e) performing a frequency analysis of sequences from step d), f) selecting desired VH sequences, g) providing a host cell with at least one vector comprising at least one of said desired VH sequences and/or at least one VL sequence of said limited VL repertoire, h) culturing said host cells and allowing for expression of VH and/or VL polypeptides, i) obtaining said binding molecules. Alternatively, step c) and d) can be replaced or supplemented by the alternative steps c′ and d′: c′) constructing a cDNA library that is screened for VH region specific DNA sequences by probing with a nucleic acid probe specific for VH regions sequences and d′) at least partial sequencing of clones containing VH inserts. Where VH or VL is mentioned, functional derivatives and/or fragments thereof are also envisaged.



BRIEF DESCRIPTION OF THE FIGURES
FIG. 1 depicts the 4 mouse CH1 reverse primers and their position within the CH1 region are highlighted (1) Mouse CH1rev0; (2) CH1rev1; (3) CH1rev2; (4) CH1rev3. FIG. 1 discloses SEQ ID NOS 158 and 157, respectively, in order of appearance.
FIG. 2 depicts the VH amplification with forward primer DO_1177 and various CH1 reverse primers. Lane 1: DO_1171; Lane 2: DO_1172; Lane 3: DO_1173; Lane 4: DO_1174; Lane 5: negative control.
FIG. 3 is a graph showing the by-stander immune response against Fc portion of Fc-EGFR fusion protein upon immunization.
FIG. 4 depicts the ErbB2 specific IgG serum titer in ErbB2 vaccinated mice.
FIG. 5 depicts the HA specific IgG serum titer in HA vaccinated mice.
FIG. 6 depicts the relative affinity of the anti-ErbB2 IgG polyclonal sera of ErbB2 vaccinated mice as determined by ELISA.
FIG. 7 is a graph showing the comparison of the percentages IgG B cells per total iLN B cells per vaccination strategy and per antigen.
FIG. 8 shows a native SDS-PAGE analysis of a protein A purified single VL human bispecific antibody preparation. MW: molecular weight. Lane 1, anti-thyroglobulin×anti-fibrinogen bispecific.
FIG. 9 depicts a native mass spectrometry analysis of a protein A purified single VL human bispecific antibody for fibrinogen and thyroglobulin produced by co-transfection of HEK293 cells. The main peak of 144352 Da is the heterodimeric bispecific IgG species (96% of total IgG); whereas the minor peaks of 144028 Da (3% of total IgG) and 142638 Da (1% of total IgG) are the homodimeric parental IgG species.
FIG. 10 is a graph showing the potency of EGFR-specific mAb to rescue A431 cells from EGF-induced cell death.



DETAILED DESCRIPTION
The term ‘obtaining nucleic acids’ as used in step c) herein includes any methods for retrieving the sequence of nucleotides of nucleic acids encoding VH sequences.
The term ‘defined population of binding molecules’ as used herein refers to at least two binding molecules that bind to at least one selected antigen or epitope of interest. Preferably, the defined population of binding molecules comprises at least a significant portion, preferably at least the majority and most preferably essentially all specific binding molecules directed against the antigen or epitope of interest generated during an immune response. More preferably a population of binding molecules comprises between hundred and several thousand binding molecules, representing a significant portion of unique antigen-specific antibodies present in, e.g., a mouse immunized with said antigen. The population of binding molecules of the present invention may have different specificities and/or affinities; i.e. may bind to different epitopes of the selected antigen or may bind to the same epitope with differing affinities. The term ‘binding molecule’ as used herein means a molecule comprising a polypeptide containing one or more regions, preferably domains, which bind an epitope on an antigen. In a preferred embodiment, such domains are derived from an antibody.
The term ‘antibody’ as used herein means a protein containing one or more domains that bind an epitope on an antigen, where such domains are derived from or share sequence homology with the variable region of an antibody. Antibodies are known in the art and include several isotypes, such as IgG1, IgG2, IgG3, IgG4, IgA, IgD, IgE, and IgM. An antibody according to the invention may be any of these isotypes, or a functional derivative and/or fragment of these. Examples of antibodies according to the invention include full length antibodies, antibody fragments, bispecific antibodies, immunoconjugates, and the like. Antibody fragments include Fv, scFv, Fab, Fab′, F(ab′)2 fragments, and the like. Antibodies according to the invention can be of any origin, including murine, of more than one origin, i.e. chimeric, humanized, or fully human antibodies. Where the term functional fragment and/or derivative is used in this specification it is intended to convey that at least one of the functions, preferably the characterizing functions of the original molecule are retained (in kind not necessarily in amount). Antibody binding is defined in terms of specificity and affinity. The specificity determines which antigen or epitope thereof is bound by the binding domain. The affinity is a measure for the strength of binding to a particular antigen or epitope. Specific binding is defined as binding with affinities (KD) of at least 1×10−5M, more preferably 1×10−7 M, more preferably higher than 1×10−9M. Typically, monoclonal antibodies for therapeutic application may have affinities of up to 1×10−10 M or even higher.
The term ‘antigen’ as used herein means a substance or molecule that, when introduced into the body, triggers the production of an antibody by the immune system. An antigen, among others, may be derived from pathogenic organisms, tumor cells or other aberrant cells, from haptens, or even from self structures. At the molecular level, an antigen is characterized by its ability to be “bound” by the antigen-binding site of an antibody. In certain aspects of the present invention also mixtures of antigens can be regarded as ‘antigen’. An antigen will comprise at least one epitope. The term ‘epitope’ as used herein means the part of an antigen that is recognized by the immune system, specifically by antibodies, B cells, or T cells. Although epitopes are usually thought to be derived from non-self proteins, sequences derived from the host that can be recognized are also classified as epitopes/antigens.
A ‘population of B cells’ as used herein may be any collection of B cells. The B cells may be present as a subpopulation of other cells in a sample. It may be derived from one or more individuals from the same or different species. Preferably, the term ‘population of B cells’ means a group of B cells obtained from at least one animal, preferably obtained from lymphoid organs. In a preferred embodiment, a population of B cells comprises essentially all splenic B cells. In a more preferred embodiment, the population further comprises also essentially all B cells obtained from at least one lymph node. In a most preferred embodiment, the population of B cells comprises B cells obtained from spleen, at least one lymph node, blood and/or bone marrow. In a particularly preferred embodiment, a population of B cells comprises essentially all B cells obtained from one or more lymphoid organs that harbor the B cells that have clonally expanded as a result of antigen stimulation. Methods to obtain such B cell populations are known in the art. Of this population of B cells, essentially all B cells carry at least one VL. In a preferred embodiment, essentially all B cells in the population of B cells express a limited VL repertoire. In a most preferred embodiment, essentially all B cells in the population of B cells carry the same VL.
The term ‘limited VL repertoire’ used herein means a restricted cohort of VL regions that supports the generation of a robust immune response upon immunization and allows the efficient assembly of original VH/VL pairs with VH regions identified through the construction of heavy chain CDR3 heat maps. In one embodiment the limited VL repertoire comprises no more than 100 different VL regions. When these 100 different VL regions will be matched with a cohort of 100 different, grouped, VH regions it will result in 104 combinations (100 times 100) of VH/VL pairs that can still be screened in functional assays. In a more preferred embodiment, the limited VL repertoire comprises no more than 10, more preferably no more than 3 or 2 different VL regions, thereby further limiting the number of different VH/VL combinations and increasing the frequency of original VH/VL pairs. In the most preferred embodiment, the limited VL repertoire comprises no more than a single VL region. The advantage of a single VL is that all antibodies that are generated upon encounter with an antigen share the same VL and are diversified only in the VH usage. A VL is defined by the particular combination of germline V and J gene segments and CDR3 region and includes somatically mutated variants of said VL. Thus, the population of B cells expressing a limited VL repertoire can mean that essentially no more than 100 VL, or preferably less than 50 VL, or more preferably less than 10, or 3 or 2 VL or most preferably a single VL is expressed. In a preferred embodiment, all VLs in the limited VL repertoire are resistant to DNA rearrangements and/or somatic hypermutations, preferably, the VL have a germline sequence. The preferred germline sequence is a light chain variable region that is frequently used in the human repertoire and has superior ability to pair with many different VH regions, and has good thermodynamic stability, yield and solubility. A most preferred germline light chain is O12, preferably the rearranged germline kappa light chain IgVκ1-39*01/IGJκ1*01 (nomenclature according to the IMGT database found on the web at imgt.org) or fragment or a functional derivative thereof. Such single VL is also referred to as common VL, or common light chain. Obviously, those of skill in the art will recognize that “common” also refers to functional equivalents of the light chain of which the amino acid sequence is not identical. Many variants of said light chain exist wherein mutations (deletions, substitutions, additions) are present that do not materially influence the formation of functional binding regions. A “common light chain” according to the invention refers to light chains which may be identical or have some amino acid sequence differences while retaining the binding specificity of the antibody. It is for instance possible within the scope of the definition of common light chains as used herein, to prepare or find light chains that are not identical but still functionally equivalent, e.g. by introducing and testing conservative amino acid changes, changes of amino acids in regions that do not or only partly contribute to binding specificity when paired with the heavy chain, and the like. It is an aspect of the present invention to use as single VL one identical light chain that can combine with different heavy chains to form antibodies with functional antigen binding domains (WO2004/009618, WO2009/157771, Merchant et al., 1998, Nissim et al., 1994).
The terms ‘amplification of nucleic acid sequences encoding immunoglobulin heavy chain variable regions’ and ‘(at least partially) sequencing of nucleic acids’ have their usual meanings in the art.
The term ‘frequency analysis’ has its usual meaning in the art. For a more detailed explanation of the term, see e.g. example 1 of the present invention.
The term ‘desired VH sequences’ means those VH sequences that, based on the frequency analysis, are produced in response to exposure to an antigen. These are assumed to encode VH regions that are specific for said antigen. Typically, an immune response to an antigen in a mouse entails the activation of about 100 different B cell clones (Poulsen et al., J Immunol 2011; 187; 4229-4235). Therefore, it is most preferred to select the 100 most abundant clones. More practically, at least 20, preferably about 50 abundant clones are selected.
‘Host cells’ according to the invention may be any host cell capable of expressing recombinant DNA molecules, including bacteria, yeast, plant cells, eukaryotes with a preference for mammalian cells. Particularly when larger antibody formats are desired bacterial cells are not suitable and mammalian cells are preferred. Suitable mammalian host cells for expression of antibody molecules are known in the art.
It is an aspect of the invention to provide a method according to the invention as described above, further comprising taking a sample of said cultured cells, the sample comprising at least one of said binding molecules, and subjecting the samples to at least one functional assay, and selecting at least one cell that expresses a binding molecule with desired characteristics.
A ‘functional assay’ as used herein means a test to establish properties such as binding specificity, affinity, neutralizing activity, tumor cell killing, proliferation inhibition or any other desired functional characteristic or activity of the binding molecule produced according to the methods of the invention. Such assays are used to determine early on whether binding molecules obtained are suitable for the desired purpose. Said desired purpose may be a diagnostic and/or therapeutic application. In one embodiment of the invention, the method for producing a defined population of binding molecules further comprises the step of harvesting the supernatants of the cultured cells, the supernatants containing said binding molecules, and subjecting the supernatants to at least one functional assay. Irrespective of a functional assay as described above, the present invention also compasses ways to determine the identity of the binding molecules, using methods known in the art.
It is an aspect of the invention to provide a method according to the invention as described above, further comprising providing said host cell with means for expression of said at least one VH and VL in a desired format. The term ‘desired formats’ as used herein refers to a form of the binding molecule in which it can be used for its particular purpose. The typical formats of binding molecules, in particular antibody like molecules that are suitable for particular purposes are well known in the art. For therapy these molecules would typically be fully human monoclonals (mono- or bispecific, and/or mixtures thereof, i.e. Oligocionics®). For imaging these molecules would typically be antibody fragments, and so on. Desired formats include, but are not limited to, bispecific formats such as DARTSTM, BiTEsTM, single light chain bispecific antibodies (Merchant et al., 1998) including CH3-engineered bispecifics such as knob-into-hole variants or charge-engineered CH3 variants, DVD-Ig antibodies (Wu et al., 2007), mixtures of antibodies (de Kruif et al., 2009) and the like.
In a further preferred embodiment, the population of B cells that is provided for the method for producing a defined population of binding molecules is enriched for B cells that express immunoglobulin receptors that bind to the antigen. Such enrichment may occur when a B cell encounters antigen (e.g. when a mouse is immunized with an antigen) and is activated to divide to generate a clone of B cells. It is an object of the present invention to provide a method for producing a population of defined binding molecules further providing B cell clones wherein said collection of B cell clones comprise a collection of VH regions that are enriched for VH regions encoding antibodies directed to the antigen or epitope of interest. Such enrichment can, for example, be carried out by taking only those B cell clones obtained from antigen-exposed animals as a starting population that are selected through an antigen recognition process, i.e. by using selection methods comprising coated or labeled antigen. Typically, the 20 most abundant clones, preferably, the 50 most abundant clones, more preferably the 100 most abundant clones are selected; most preferably, the 200 most abundant clones are selected.
In a preferred embodiment, the antigen-specific VH regions are clonally related. In another preferred embodiment, the population of B cells is highly-enriched for B cells that express immunoglobulin receptors that bind to the antigen. In this case, the majority of B cells that are provided will be antigen specific, and thus, an amplification step of all nucleic acid sequences encoding VH regions may not be necessary and all isolated nucleic acids from said B cells can be, at least partially, sequenced directly. It is thus an aspect of the present invention, to provide for a method for producing a defined population of binding molecules, said method comprising at least the step of providing a population of B cells expressing a limited VL repertoire wherein said B cells comprise a collection of VH regions that is enriched for VH regions encoding antibodies directed to the antigen or epitope of interest.
It is an aspect of the invention to provide a method according to the invention, wherein said population of B cells is obtained from a transgenic mouse carrying a limited, preferably human, VL repertoire.
It is an aspect of the invention to provide a method according to the invention, wherein said mouse has been immunized such that selective clonal expansion of B cells that react with the antigen or epitope of interest is preferentially induced.
An ‘immunization protocol that causes the selective expansion of B cells’ as used herein means that primary and booster immunizations are designed to cause selective expansions of B cells that produce antibodies that bind to the antigen or epitope of interest. The immunization protocol may for example use different forms or fragments of the antigen during primary immunization and each subsequent booster immunization. For example, the antigen may be expressed on the membrane of a cell, a recombinant protein, a recombinant protein fused to another protein, a domain of a protein or a peptide of a protein. The immunization protocol may include the use of an adjuvant during the primary and/or booster immunizations. In a preferred embodiment, an adjuvant is used during primary immunization only to limit the extent of non-specific expansion of bystander B cells. Bystander B cells are cells that are activated without the step of binding of antigen to the antibody receptor expressed on the surface of the B cell. It is known in the art that immunization with Fc-fusion proteins for example, often results in a robust anti-Fc response where up to about 70% of all B cells react to the Fc part of the fusion protein rather than to the antigen of interest. In the most preferred embodiment, an immunization protocol is used without adjuvant to preferentially expand B cells that have been activated by the antigen used for immunization. It is therefore an aspect of the invention to provide a method for producing a defined population of binding molecules, said method comprising at least the step of providing a population of B cells expressing a limited VL repertoire, wherein said population of B cells is obtained from a transgenic mouse carrying a limited, preferably human, VL repertoire, wherein said mouse has been immunized with an antigen, such that selective clonal expansion of B cells that react with the antigen or epitope of interest is preferentially induced. A preferred way of inducing selective clonal expansion of B cells is DNA tattoo vaccination. The term ‘DNA tattoo vaccination’ refers to an invasive procedure involving a solid vibrating needle loaded with plasmid DNA that repeatedly punctures the skin, wounding both the epidermis and the upper dermis and causing cutaneous inflammation followed by healing (Bins 2005/Pokorna 2008).
In transgenic mice with human antibody genes, a plurality of human IgH V regions and/or a plurality of human Ig light chain kappa V regions have been introduced in the genome of the animals (Lonberg 2005). Upon immunization, these mice mount an antigen-specific immune response that is diversified in heavy and light chain V region utilization. It is anticipated that, upon immunization of these transgenic mice with antigen, high throughput sequencing, frequency ranking of VH and VL genes, construction of CDR3 heat maps and random or frequency-guided pairing of VH and VL regions yields a large proportion of antibodies that do not bind to the antigen or bind with low affinity (Reddy et al., 2010). It is therefore an object of the present invention to use transgenic animals that harbor a restricted repertoire of human immunoglobulin light chains for immunization purposes. Such transgenic animals that harbor a limited repertoire of human light chains are described in WO2009/157771. Preferably, the endogenous kappa light chain is functionally silenced in such animals to minimize the use of murine light chains in antibodies generated in such mice. In a further embodiment, also the endogenous lambda light chain is functionally silenced to further reduce the use of murine light chains in antibodies. In a most preferred embodiment, transgenic animals that carry a single rearranged human VL region that is resistant to somatic hypermutation is used for immunization to generate antibodies in which the processes of somatic mutation and clonal expansion and selection mainly act on the VH regions of the antibody expressed on the membrane of a B cell. Hence, high throughput sequencing and creation of CDR3 heat maps to identify antigen-driven, clonally expanded B cells and the antibodies they encode may focus on VH regions only. It is therefore an aspect of the present invention to provide a method for producing a defined population of binding molecules, said method comprising the step of providing a population of B cells, wherein said population of B cells is obtained from a transgenic animal, preferably a mouse, carrying a limited, preferably human, VL repertoire, wherein said animal has been immunized with an antigen. In a preferred embodiment, the invention provides for a method for producing a defined population of binding molecules, said method comprising the step of providing a population of B cells, wherein said population of B cells is obtained from a transgenic mouse carrying a single rearranged human VL, preferably the human IGVκ1-39 light chain (WO2009/157771). The advantage of this germline human IGVκ1-39 light chain is its anticipated reduced immunogenicity due to absence of strong non-self DRB1 binders (WO2009/157771, example 19). In addition, this light chain is known to be capable of pairing with many different human VH regions. Through an array of genetic mechanisms, the antibody VL repertoire that can be generated in an animal is virtually unlimited.
In one aspect according to the invention, the method for producing a defined population of binding molecules further comprises the step of providing the host cell with means for expression of the at least one VH and VL in a desired format. The term ‘desired format’ as used herein means that the selected VH and VL sequences are expressed together with other sequences such that antibody formats can be expressed within the host cell. In one embodiment, after selection of suitable VHs, mixtures of antibodies are produced by a single cell by introducing at least 2 different heavy chains and one common light chain into a cell (WO2004/009618). In another embodiment, after selection of suitable VH regions, the at least two different heavy chains are engineered such that heterodimerization of heavy chains is favored over homodimerization. Alternatively, the engineering is such that homodimerization is favored over heterodimerization. Examples of such engineered heavy chains are for example the protuberance and cavity (knob-into-hole) constructs as described in WO98/050431, or the charge-variants as described (Gunasekaran et al., 2010) or WO2009/089004, or WO2006/106905.
It is another aspect of the invention to provide a method for producing a defined population of binding molecules, wherein said binding molecules have a desired effect according to a functional screening assay, the method further comprising the step of taking the supernatants of said cultured cells, the supernatants comprising said binding molecules, subjecting the supernatants to at least one functional screening assay, and selecting at least one cell that expresses a binding molecule with desired characteristics. Preferably, said host cell comprises a nucleic acid sequence encoding a common light chain that is capable of pairing with said desired VH, such that produced antibodies comprise common light chains, as described above. In specific embodiments said culturing step and said screening step of the method is performed with at least two clones. The method may optionally include an assay for measuring the expression levels of the antibodies that are produced. Such assays are well known to the person skilled in the art, and include protein concentration assays, immunoglobulin specific assays such as ELISA, RIA, and the like.
The present invention inter alia describes a method for producing a defined population of binding molecules, wherein the starting point is a population of B cells that expresses a restricted repertoire of light chain variable (VL) regions and a diversified repertoire of heavy chain variable regions (VH). The VL region repertoire may for example be restricted by limiting the number of V and J genes available during recombination in a transgenic animal or by inserting one or a few pre-rearranged VL regions in the genome of a transgenic animal, by reducing or abrogating the rate of somatic mutation occurring in the VL region or by a combination of these strategies (WO2009/157771). The VH regions may be diversified by recombination of V, D and J gene segments and somatic mutation. Upon immunization, collections of heavy chain nucleic acid sequences are obtained from B cells in the lymphoid organs of transgenic animals, subjected to high throughput sequencing and analyzed to rank all unique heavy chains based on frequency and to rank HCDR3 based on length, percentage identity and frequency to construct HCDR3 heat maps. Nucleotide sequence information is used to rank VH regions according to their frequency in the collection and frequently occurring sequences, assumingly representing VH regions expressed in B cells that have undergone clonal expansion as a result of antigen stimulation, are cloned into expression vectors in conjunction with one of the VL regions present in the restricted repertoire. By using a restricted VL repertoire, the search for original VH/VL pairs is highly simplified because no VL sequence information needs to be retrieved, analyzed or ranked from the immunized animal; in case the original restricted VL repertoire comprises a single VL, all VH/VL combinations will represent original pairs as used by B cells in vivo. In case the original restricted VL repertoire contained a few VL regions, combination with the ranked VH regions yields only small collections of VH/VL combinations that can be rapidly screened for binding and functional activity.
Expression vectors containing the VH and VL regions are used to transfect cells to rapidly obtain antibodies for binding assays and functional screening. Different formats of antibodies can be obtained by using expression vectors that contain different genetic elements that, for example, drive the formation of antibody fragments or antibodies with different isotypes, bispecific antibodies, mixtures of antibodies or antibodies that have engineered variable or constant regions for modified effector functions or modified half life, or contain additional binding sites, are devoid of amino acid sequences that have a deleterious effect on development, production or formulation of antibodies such as glycoslylation and deamidation sites.
EXAMPLES
Example 1
Deep Sequence Analysis and Frequency Ranking of VH Genes Expressed in Murine Spleen B Cells Using VH Family-specific Primers
This example describes the use of high throughput sequencing to retrieve and analyze the repertoire of antibody VH regions expressed in the spleen of wild type mice immunized with the antigens ErbB2 or ErbB3. Because immunization will enrich the B cell population for clones directed against the immunogen, it is anticipated that sequencing large numbers of VH transcripts identifies these B cell clones as they will be present within the population in higher frequencies. In this example, approximately 25,000 VH region genes from the spleen of a single immunized mouse are retrieved by high throughput sequencing and ranked based on frequency.
Spleens were collected from mice immunized with either the antigen ErbB2 or ErbB3 using DNA tattooing (see example 6). A single cell suspension was prepared according to standard techniques. B cells were isolated from this splenic single cell suspension in a two-step MACS procedure using materials from Miltenyi biotec (http://www.miltenyibiotec.com/en/default.aspx). Briefly, splenic B cells were isolated by first depleting the non-B cells, followed by positive selection of B cells. The non-B cells were depleted by labeling of T cells, NK cells, myeloid cells, plasma cells and erythrocytes with a cocktail of biotinylated antibodies (Table 1) and subsequent incubation with streptavidin Microbeads. Next the non-B cells were depleted over an LD column. The flow through, containing the enriched B cell fraction, was labeled with magnetic Microbeads using FITC conjugated anti-lgG1 and IgG2ab antibodies (Table 1) followed by labeling with anti-FITC Microbeads (Miltenyi Biotec, Cat no. 130-048-701). The IgG labeled cells were subsequently positively selected over an LS column (Miltenyi Biotec, Cat no 130-042-401). MACS procedures were performed according to Kit/Microbead specific manuals supplied by Miltenyi. The purity of the isolated B cells was determined by FACS analysis according to standard techniques using the antibodies listed in Table 2.







TABLE 1







antibodies to label non-B cells












Ab #
Antigen
Label
Clone
Supplier
Cat no.





Ab0064
IgG1
FITC
A85-1
Becton Dickinson
553443


Ab0131
IgG2ab
FITC
R2-40
Becton Dickinson
553399


Ab0158
CD138
Biotin
281-2
Becton Dickinson
553713


Ab0160
CD3E
Biotin
145-2C11
eBioscience
13-0031


Ab0161
Ly-6G
Biotin
RB6-8C5
eBioscience
13-5921


Ab0162
TER-119
Biotin
TER-119
eBioscience
13-5921


Ab0163
CD49b
Biotin
DX5
eBioscience
13-5971


Ab0164
CD11b
Biotin
M1/70
eBioscience
13-0112
















TABLE 2







antibodies for FACS analysis












Ab #
Antigen
Label
Clone
Supplier
Cat no.















Ab0064
IgG1
FITC
A85-1
Becton Dickinson
553443


Ab0131
IgG2ab
FITC
R2-40
Becton Dickinson
553399


Ab0067
CD138
APC
281-2
Becton Dickinson
558626


Ab0160
IgM
PE-CY7
II/41
eBioscience
25579082


Ab0161
IgD
PE
11-26
eBioscience
12599382


Ab0162
CD19
PerCP-cy5.5
1D3
eBioscience
45019382


Ab0163
B220
Aallphycocyaninefluor 780
RA3-6B2
eBioscience
47045282









To extract the nucleic acids of the B cells, cells were Iysed in Trizol LS (Invitrogen). RNA was prepared and cDNA synthesized according to standard techniques. Primers designed for amplification of murine VH repertoires were taken as starting material and were modified for use in 454 high throughput sequencing by addition of 454 primer sequences (forward 454 primer: CGTATCGCCTCCCTCGCGCCATCAG (SEQ ID NO: 1); reverse 454 primer: CTATGCGCCTTGCCAGCCCGCTCAG) (SEQ ID NO: 2). The complete primer sequences for the PCR amplification of murine VH repertoires are shown in Tables 3 and 4.







TABLE 3







The forward 454 Phusion primers, complete. The phusion part


(CGTATCGCCTCCCTCGCGCCATCAG) (SEQ ID NO: 1)is in italic, in bold 


is the 5′part of the VH genes.










Name
sequence
SEQ ID NO:
wobble













mIGHV1A_454
CGTATCGCCTCCCTCGCGCCATCAGGAGKTCMAGC
3
K = 15%T/85%G



TGCAGCAGTC

M = 15%A/85%C


 


mIGHV1B_454
CGTATCGCCTCCCTCGCGCCATCAGSAGRTCCASCT
4
S1 = 5%G/95%C



GCAGCAGTC

R = 5%A/95%G





S2 = 95%G/5%C


 


mIGHV1C_454
CGTATCGCCTCCCTCGCGCCATCAGSAGGTCCAGCT
5
S = 50%C/50%G



HCAGCAGTC

H = 33%A/33%C/





33%T


 


mIGHV1D_454
CGTATCGCCTCCCTCGCGCCATCAGSAGRTCCAGCT
6
S = 80%G/20%C



GCAACAGTC

R = 15%A/85%G


 


mIGHV1E_454
CGTATCGCCTCCCTCGCGCCATCAGCAKGTCCAACT
7
K = 15%T/85%G



GCAGCAGCC




 


mIGHV1F_454
CGTATCGCCTCCCTCGCGCCATCAGCAGGCTTATCT
8




ACAGCAGTC




 


mIGHV1G_454
CGTATCGCCTCCCTCGCGCCATCAGCAGCGTGAGCT
9




GCAGCAGTC




 


mIGHV2_454
CGTATCGCCTCCCTCGCGCCATCAGCAGGTGCAGM
10
M = 15%A/85%C



TGAAGSAGTC

S = 50%C/50%G


 


m1GHV3_454
CGTATCGCCTCCCTCGCGCCATCAGSAKRTGCAGCT
11
S = 80%G/20%C



TCAGGAGTC

K = 50%G/50%T





R = 15%A/85%G


 


m1GHV4_454
CGTATCGCCTCCCTCGCGCCATCAGGAGGTGAAGC
12




TTCTCCAGTC




 


mIGHV5A_454
CGTATCGCCTCCCTCGCGCCATCAGGAAGTGMWGC
13
M = 15%A/85%C



TGGTGGAGTC

W = 80%A/20%T


 


mIGHV5B_454
CGTATCGCCTCCCTCGCGCCATCAGGAVGTGAAGCT
14
V = 20%C/40%G/



SGTGGAGTC

40%A





S = 80%G/20%C


 


mIGHV6A_454
CGTATCGCCTCCCTCGCGCCATCAGGAAGTGAARM
15
R = 50%A/50%G



TTGAGGAGTC

M = 50%A/50%C


 


mIGHV6B_454
CGTATCGCCTCCCTCGCGCCATCAGGATGTGAACCT
16




GGAAGTGTC




 


mIGHV6C_454
CGTATCGCCTCCCTCGCGCCATCAGGAGGAGAAGC
17




TGGATGAGTC




 


mIGHV7_454
CGTATCGCCTCCCTCGCGCCATCAGGAGGTGMAGC
18
M = 50%A/50%C



TGRTGGAATC

R = 50%A/50%G


 


mIGHV8_454
CGTATCGCCTCCCTCGCGCCATCAGCAGRTTACTC
19
R = 50%A/50%G



WGAAASAGTC

W = 20%A/80%T





S = 80%G/20%C


 


mIGHV9_454
CGTATCGCCTCCCTCGCGCCATCAGCAGATCCAGTT
20
S = 80%G/20%C



SGTRCAGTC

R = 15%A/85%G


 


mIGHV10_454
CGTATCGCCTCCCTCGCGCCATCAGGAGGTGCAGC
21




TTGTTGAGTC




 


mIGHV11_454
CGTATCGCCTCCCTCGCGCCATCAGGAAGTGCAGCT
22




GTTGGAGAC




 


mIGHV13_454
CGTATCGCCTCCCTCGCGCCATCAGSAGGTGCAGCT
23
S = 50%C/50%G



KGTAGAGAC

K = 50%G/50%T


 


mIGHV15_454
CGTATCGCCTCCCTCGCGCCATCAGCAGGTTCACCT
24




ACAACAGTC
















TABLE 4







The reverse 454 Phusion primers, complete.


The phusion part (CTATGCGCCTTGCCAGCCCGCTCAG) 


(SEQ ID NO: 2) is in italic. The part specific 


for the murine J segments are in bold.











SEQ


Name
sequence
ID NO:





mIGHJ1_454
CTATGCGCCTTGCCAGCCCGCTCAGGAGGAGACG
25



GTGACCGTGGTCCC



 


mIGHJ2b_454
CTATGCGCCTTGCCAGCCCGCTCAGGAGGAGACT
26



GTGAGAGTGGTGCC



 


mIGHJ3_454
CTATGCGCCTTGCCAGCCCGCTCAGGCAGAGACA
27



GTGACCAGAGTCCC



 


mIGHJ4b_454
CTATGCGCCTTGCCAGCCCGCTCAGGAGGAGACG
28



GTGACTGAGGTTCC









In the PCR, the four reverse JH primers were mixed in equal ratios prior to use. The forward primers were not mixed, so 22 PCR reactions were performed. The PCR reaction products were analyzed on gel and it was expected to yield PCR products of 350 to 400 base pairs in length. Some PCR products were mixed based on frequency of VH genes in normal repertoires (Table 5, below). The intensities of the bands on gel were expected to correspond to the ratios listed in this table and when this was the case, PCR products were mixed for sequencing based on volumes. Where intensities of bands on gel did not correspond to ratios listed in Table 5, over- or under represented bands could be adjusted. As high throughput sequencing also identifies the primer and the PCR reaction, ratios can always be ‘adjusted’ after sequencing and the frequency of each VH gene per PCR reaction analyzed.







TABLE 5







PCR products that can be pooled.










Pool name
Percentage (%)













1A
39



1B




1CD




1EFG




2
4



3⁄4
7



5
15



6
1



7/8
10



9
16



10/11
6



13/15
2









Sequence analysis was performed in order to rank all unique VH genes from a single animal, immunization, and/or cell population on frequency:

    
    
        Raw sequences were analyzed to identify those that encode a VH region open reading frame that at least contains HCDR3 plus some neighboring framework sequence to identify the VH. Preferably, the VH region open reading frame contains CDR1 to framework 4
        All sequences were translated into amino acid sequences
        All sequences were clustered based on identical HCDR3 protein sequence
        All clusters were ranked based on number of VH sequences in each cluster
        An alignment of all sequences in each cluster was made based op protein sequences, in which differences with the germline VH and germline JH are indicated. All identical sequences in the alignment are again clustered.
    
    


This provides information on the most frequently occurring VH gene within a CDR3 cluster. This gene may have differences compared to the germline as a result of somatic hypermutation. This gene is chosen for construction and expression with the common VL gene
High throughput sequencing was performed using Roche 454 sequencing on samples from an individual immunized mouse. Other high throughput sequencing methods are available to a skilled person and are also suitable for application in the method. In total, 118,546 sequence reads were used as a raw data set to first identify sequences that represented full length VH regions or portions thereof encoding at least 75 amino acids. For each sequence within this set, frameworks 2-4 and all 3 CDR regions were identified as described (Al-Lazikani et. al., 1997). Sequences were subsequently subjected to a number of criteria including the presence of a canonical cysteine residue, the absence of stop codons and the minimal length for each CDR regions. All VH regions fulfilling these criteria were then clustered to identify the frequency in which each unique CDR3 are used, thereby generating heavy chain CDR3 heat maps. Then, all identical clones in each CDR3 cluster were grouped and aligned with the germ line VH sequence. This analysis allows for the identification of abundantly used VH genes in large repertoires.
Albeit that this analysis can be carried out manually, the use of an algorithm including the above instructions greatly facilitates the analysis process (Reddy et al., 2010)
A total of 18,659 clusters were identified within 30,995 annotated VH sequences and 2,733 clusters were found that had more than 1 member. In addition, 123 clusters had more than 20 members. The first 40 clusters of these are shown in Table 6. Nine clusters had more than 100 members. The number of 30.000 sequences is more than sufficient as many clones appear only once and the experiments are aimed at identifying frequent clones. In fact, less than 30.000 sequences would work quite well. Alignments of the two largest clusters demonstrated the presence of 100% germline genes and variants containing mutations throughout the VH gene (data not shown).







TABLE 6







Example of clusters identified by unique CDR3.











# identical




cluster #
sequences
HCDR3
SEQ ID NO:













Cluster001
337
YSNYWYFDV
29


 


Cluster002
212
GGLRGYFDV
30


 


Cluster003
130
YDSNYWYFDV
31


 


Cluster004
124
TYDNYGGWFAY
32


 


Cluster005
116
AGLLGRWYFDV
33


 


Cluster006
116
RDY



 


Cluster007
113
RFGFPY
34


 


Cluster008
103
AITTVVATDY
35


 


Cluster009
102
AYYYGGDY
36


 


Cluster010
 99
SGPYYSIRYFDV
37


 


Cluster011
 91
SEGSSNWYFDV
38


 


Cluster012
 89
GTLRWYFDV
39


 


Cluster013
 76
DFYGSSYWYFDV
40


 


Cluster014
 75
DNWDWYFDV
41


 


Cluster015
 73
FYDYALYFDV
42


 


Cluster016
 72
GNYGSSYFDY
43


 


Cluster017
 72
WKVDYFDY
44


 


Cluster018
 70
GGYWYFDV
45


 


Cluster019
 69
YKSNYWYFDV
46


 


Cluster020
 66
LLPYWYFDV
47


 


Cluster021
 64
SYYGSSYWYFDV
48


 


Cluster022
 63
GGYYGSRDFDY
49


 


Cluster023
 63
DYDWYFDV
50


 


Cluster024
 61
TYNNYGGWFAY
51


 


Cluster025
 57
GGLYYDYPFAY
52


 


Cluster026
 57
WGDYDDPFDY
53


 


Cluster027
 56
DYYGSSYWYFDV
54


 


Cluster028
 55
EATY
55


 


Cluster029
 52
YGSSYWYFDV
56


 


Cluster030
 52
WGYGSKDAMDY
57


 


Cluster031
 51
WGRELGNYFDY
58


 


Cluster032
 49
YGNYWYFDV
59


 


Cluster033
 48
TVTTGIYYAMDY
60


 


Cluster034
 48
HYYSNYVWWYFDV
61


 


Cluster035
 47
GALRGYFDV
62


 


Cluster036
 47
HYYGSTWFAY
63


 


Cluster037
 45
LGAYGNFDY
64


 


Cluster038
 44
REFAY
65


 


Cluster039
 43
EAAYYFDY
66


 


Cluster040
 43
GSLRGYFDV
67









Example 2
Deep Sequence Analysis and Frequency Ranking of VH Genes Expressed in Murine IgG+ Spleen B Cells Using a Single Primer Set
In this example, a primer specific for the IgG CH1 constant region was used to interrogate the repertoire of VH gene sequences expressed in IgG+ memory B cells in the spleen of mice immunized with the ErbB2-Fc fusion protein. At the 5′ end of the mRNA an oligonucleotide primer was annealed to a triple guanine stretch that was added to each mRNA by an MMLV reverse transcriptase. This 5′ primer introduces a priming site at the 5′ end of all cDNAs. Using this approach, amplifications of all VH regions expressed in IgG+ B cells can be done using the 5′ primer and the CH1 primer, preventing a potential bias introduced by the use of a large number of VH family-specific primers and focusing the analysis on a population of B cells that has apparently undergone activation and isotype switching as a result of stimulation with antigen.
Wild-type C57BL/6 mice were immunized intraperitoneally with ErbB2-Fc protein (1129ER, R&D systems) dissolved in Titermax Gold adjuvant (TMG, Sigma Aldrich, T2684) on days 0, 14, and 28 and with ErbB2-Fc in PBS at day 42. Total splenic B cells were purified on day 45 by MACS procedure as detailed in Example 1. The total splenic B cell fraction from successfully-immunized mice, as determined by serum antibody titers in ELISA, contained 5-10% IgG+ B cells. This material was used to optimize the PCR conditions.
Transgenic mice containing the human HuVκ1-39 light chain, as described in (WO2009/157771) were either immunized with EGFR-Fc fusion protein (R&D Systems, Cat no 334-ER) emulsified in Titermax Gold adjuvant (TMG, Sigma Aldrich, T2684) or, as a control, with Titermax Gold adjuvant emulsified with PBS at an interval of 14 days. The latter group was included to identify the VH repertoire of B cells responding to the adjuvant alone. Mice were three times immunized with EGFR-Fc/adjuvant emulsion or adjuvant emulsion alone on days 0, 14 and 28. At day 35 the anti-EGFR serum titer was determined by FACS using standard procedures. Mice that had developed at day 35 a serum titer >1/1.000 received a final intraperitoneal boost with EGFR-Fc protein dissolved in PBS at day 42 followed by collection of spleen at day 45 for isolation of splenic B cells. Splenic B cells were isolated from the total spleen by positive selection using mouse CD19-specific magnetic beads. The splenic B cell fraction was Iysed in Trizol LS to isolate total RNA.
After RNA isolation cDNA was prepared (cell populations from HuVκ1-39 light chain transgenic mice and mock and control/test sample with similar cell population) using MMLV reverse transcriptase in the presence of primers mouse CH1 rev 0, 1, 2 or 3 together with MMLV 454 fw (Table 7). Four different primers were tested to identify the one that resulted in optimal cDNA yields and PCR products; MMLV was designed to contain the 3′ GGG stretch similar to the Clontech primers, deleting cloning sites but adding 454 sequences (SMARTerTM RACE cDNA Amplification Kit, Cat#634924, Clontech).
As a control, cDNA was also prepared using the standard Clontech protocol; the SMARTer PCR cDNA synthesis kit using the SMARTer II A oligo together with the gene specific CH1 rev 0, 1, 2 or 3 primers (SMARTerTM RACE cDNA Amplification Kit, Cat#634924, Clontech). Using standard procedures, the optimal PCR cycle number and optimal primer combination were determined. The Clontech protocol was followed for PCR conditions.
Next, material from immunized HuVκ1-39 light chain transgenic mice and mock-immunized mice was amplified under optimal conditions. Preferably amplifications were carried out with a reverse primer upstream from the primer used in cDNA synthesis to obtain a more specific PCR product (nested PCR). cDNA was cloned in pJET according to the manufacturer's instructions (CloneJET PCR cloning kit, Fermentas #K1232) and Sanger-sequence 100 clones. PCR product of material derived from the immunized and mock-immunized animals was purified and used for 454 sequencing. The data were analyzed and used to construct CDR3 heat maps are constructed as described in example 1.







TABLE 7







Primers used in this study. Primer MMLV 454 fw hybridizes at the 5'end of the


mRNA. The position of the CH1 rev primers is indicated in FIG. 1.










number
Name
Sequence
SEQ ID NO:













DO_1165
MMLV 454 fw
  GGG
68


 


DO_1166
454 fw
 
1


 


DO_1167
mouse CH1 rev 0
TGATGGGGGTGTTGTTTTGG
69


 


DO_1168
mouse CH1 rev 1
CAGGGGCCAGTGGATAGAC
70


 


DO_1169
mouse CH1 rev 2
GCCCTTGACCAGGCATCC
71


 


DO_1170
mouse CH1 rev 3
CTGGACAGGGATCCAGAGTTC
72


 


DO_1171
mouse CH1 454 rev 0
CTATGCGCCTTGCCAGCCCGCTCAGTGATGGGGGTGTTGTTTTGG
73


 


DO_1172
mouse CH1 454 rev 1
CTATGCGCCTTGCCAGCCCGCTCAGCAGGGGCCAGTGGATAGAC
74


 


DO_1173
mouse CH1 454 rev 2
CTATGCGCCTTGCCAGCCCGCTCAGGCCCTTGACCAGGCATCC
75


 


DO_1174
mouse CH1 454 rev 3
CTATGCGCCTTGCCAGCCCGCTCAGCTGGACAGGGATCCAGAGTTC
76


 


DO_1175
smart IV oligo
AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG
77


 


DO_1176
smart IV oligo short
AAGCAGTGGTATCAACGCAGAGTGGG
78


 


DO_1177
5PCR primer 454
  AAGCAGTGGTATCAACGC
79




AGAGT





Legends:


454 5′ sequence   (SEQ ID NO: 1)


454 3′ sequence CTATGCGCCTTGCCAGCCCGCTCAG (SEQ ID NO: 2)


SMART sequence AAGCAGTGGTATCAACGCAGAGT (SEQ ID NO: 80)


SfiI GGCCATTACGGCC (SEQ ID NO: 81)






The results of these experiments yield heavy chain CDR3 heat maps that represent sequences used by B cells that have undergone activation and isotype switching as a result of stimulation by antigen. The VH region sequences present in the most frequently-occurring clusters derived from the transgenic mice containing the human IGVκ1(1-39 light chain can be used to combine with the sequence of human IGVκ1-39 light chain to collections of human monoclonal antibodies enriched for antibodies specific for EGFR.
Example 3
Deep Sequence Analysis and Frequency Ranking of VH Genes Expressed in Murine IgG+ Spleen B Cells without VH Family Specific Primers
For this example, the objective was to optimize deep sequencing technology of IgG VH genes by using amplifications based on primers that amplify all Ig heavy chains and thus to prevent potential bias introduced by VH family specific primers.
Antibody VH gene amplification for phage display library generation uses primers that append restriction sites for cloning to VH genes at the required position within the genes. This requires primer annealing sites within the VH genes and therefore VH family specific primers. In example 1 such primer sequences were used. A downside of the use of VH family primers is that they amplify a subset of all VH genes in the repertoire. As a large collection of primers and PCR reactions is used to amplify all genes and the PCR products are mixed afterwards, this will result in skewing of the ratios of the VH genes originally present in the samples and therefore over/under representation of VH genes in the final sequenced repertoire. To circumvent these problems in this study the RACE (Rapid Amplification of cDNA Ends) amplification protocol was used in combination with an IgG1-CH1 specific primer set. The SMARTer RACE kit (Clontech; cat #634923 & #634924) was used which couples a 5′ synthetic adaptor to the mRNA. The SMARTScribe RT enzyme produces a copy of the RNA transcript. The SMARTScribe RT starts the cDNA synthesis from the IgG mRNA at an anti-sense primer that recognizes a known sequence in the IgG mRNA such as the poly A tail in this study. When the SMARTScribe RT enzyme reaches the 5′ end of the IgG RNA template it adds 3 to 5 residues to the 3′ end of the first-strand cDNA. The SMARTer oligo IIA contains a terminal stretch of modified bases that anneals to this extended tail added by the SMARTScribe RT, allowing the oligo to serve as a template for the RT, Subsequently the SMARTScribe RT switches templates from the mRNA molecule to the SMARTer oligo HA, generating a complete double strand cDNA copy of the original RNA with the additional SMARTer sequence at the end. Thereafter, for PCR amplification of VH cDNA, an oligo that anneals to the 5′ SMART sequence on one end of the cDNA (5 PCRprimer 454) and an IgG-CH1 specific primer at the other end of the cDNA are applied (Table 8). In this way the VH regions from IgG heavy chains are amplified with just one primer combination, independent from VH family specific primers, and in this case a primer specific for the IgG CH1 constant region was used to interrogate the repertoire of VH gene sequences expressed in IgG+ memory B cells in the spleen of mice immunized with the ErbB2-Fc fusion protein. In the best case the IgG-CH1 specific reverse primer should anneal as close as possible to the VH gene to increase the chance that the full VH gene can be sequenced.
Samples for deep sequencing were obtained from two immunized transgenic mice carrying the human huVk1-39 light chain; mice were numbered mouse 1145 and mouse 1146. Briefly, transgenic mice containing the human HuVκ1-39 light chain, as described in (WO2009/157771) were immunized with EGFR-Fc fusion protein (R&D Systems, Cat no 334-ER) emulsified in Titermax Gold adjuvant (TMG, Sigma Aldrich, T2684). Mice were three times immunized with EGFR-Fc/adjuvant emulsion on days 0, 14 and 28. At day 35 the anti-EGFR serum titer was determined by FACS using standard procedures. Mice that had developed at day 35 a serum titer >1/1.000 received a final intraperitoneal boost with EGFR-Fc protein dissolved in PBS at day 42 followed by collection of spleen at day 45 for isolation of splenic B cells. Splenic B cells were isolated from the total spleen by positive selection using mouse CD19-specific magnetic beads. The splenic B cell fraction was Iysed in Trizol LS to isolate total RNA according to standard procedures.
cDNA was synthesized from these RNA samples using the SMARTer RACE cDNA Amplification kit according to manufacturer's instructions to come to so-called RACE-Ready cDNA. This RACE-ready cDNA was subsequently amplified by PCR according to manufacturer's instructions and the SMART specific primer (Table 8) and one of several IgG CH1 specific primers were used to establish which IgG-CH1 specific primer amplifies the IgG transcript best. The IgG-CH1 specific reverse primers DO_1171 to DO_1174 containing the 3′ 454 sequences were tested and as a forward primer the SMART tag specific primer containing the 5′ Roche 454 sequencing tag (DO_1177) was used (see table 8 and FIG. 1) (SMARTerTM RACE cDNA Amplification Kit, Cat#634923 & 634924, Clontech). The PCR schedule used is shown in Table 9.







TABLE 8







Primers used in this study. The position of the CH1 rev primers is


indicated in FIG. 1.










number
Name
Sequence
SEQ ID NO:





DO_1171
mouse CH1 454 
CTATGCGCCTTGCCAGCCCGCTCAGTGATGGGGGTGTTGTTTTGG
73



rev 0




 


DO_1172
mouse CH1 454 
CTATGCGCCTTGCCAGCCCGCTCAGCAGGGGCCAGTGGATAGAC
74



rev 1




 


DO_1173
mouse CH1 454 
CTATGCGCCTTGCCAGCCCGCTCAGGCCCTTGACCAGGCATCC
75



rev 2




 


DO_1174
mouse CH1 454 
CTATGCGCCTTGCCAGCCCGCTCAGCTGGACAGGGATCCAGAGTTC
76



rev 3




 


DO_1177
5PCR primer 
 
79



454





Legends:


454 5′ sequence   (SEQ ID NO: 1)


454 3′ sequence CTATGCGCCTTGCCAGCCCGCTCAG (SEQ ID NO: 2)


SMART sequence AAGCAGTGGTATCAACGCAGAGT (SEQ ID NO: 80)













TABLE 9







PCR schedule.












Step
Temperature
Time
Number of cycles






1
98° C.
30 seconds
 1



2
98° C.
25 seconds
10



3
72° C.-54° C.
25 seconds
Touchdown



4
72° C.
50 seconds




5
98° C.
25 seconds
14



6
58° C.
25 seconds




8
72° C.
50 seconds




9
72° C.
 3 minutes
 1



10 
16° C.
∞









PCR results are shown in FIG. 2 demonstrating that DO_1171 did not give a PCR product, whereas the other three all gave an abundant PCR product, see FIG. 2. It was concluded that DO_1172 is the best option to amplify the VH region, since this primer is closest to the VH gene and produces in PCR a clear and specific band.
The PCR was performed several times on template (Table 9) to obtain enough material (500-1000 ng) for deep sequencing. To determine the DNA concentration after PCR, a fluorimetric quantification of DNA was performed. In this case, Quant-IT Picogreen dsDNA (Invitrogen P7589) measurements were performed using a Biotek Synergy. PCR product of material derived from the immunized animals (samples 1145 and 1146) was subsequently sent to Eurofins MWG Operon (found on the web at eurofinsdna.com) for high throughput sequencing.
Eurofins first ligated barcoded linkers to each of the two samples. In this way both samples could be sequenced in one chip-segment, thereby reducing costs. With this layout Eurofins provided sequencing with GS FLX+ technology where length read is 600-700 bp on average.
Deep sequencing revealed more than 50.000 reads per mouse and data were analyzed as explained in example 1. Table 10 provides the 25 largest clusters from two of the analyzed samples.







TABLE 10





Cluster size, HCDR3 sequence and VH genes used are presented from


 cDNA samples from mouse 1145 and 1146. #/25 provides the


frequency of the VH gene in 25 clusters.







sample 1145













Cluster






Name
Size
CDR3 sequence
SEQ ID NO:
VH gene
#/25





Cluster001
 1243
HYSDYPYFDY
82
J558.66.165
19


 


Cluster002
 858
YGDYINNVDY
83
J558.66.165



 


Cluster003
 543
GFYGYDF
84
7183.19.36
 1


 


Cluster004
 376
LDTIVEDWYLDV
85
J558.66.165



 


Cluster005
 335
LDTVVEDWYFDV
86
J558.66.165



 


Cluster006
 320
YGDYSNYVDY
87
J558.66.165



 


Cluster007
 313
TRQFRLRDFDY
88
J558.83.189
 1


 


Cluster008
 290
FDYGSTQDYAMDY
89
J558.66.165



 


Cluster009
 213
SGNYDFYPMDY
90
J558.66.165



 


Cluster010
 205
RLVEY
91
J558.66.166
 1


 


Cluster011
 197
YGDYSNNVDY
92
J558.66.165



 


Cluster012
 194
LDDGYPWFAY
93
J558.55.149
 1


 


Cluster013
 182
LSDYGSSAYLYLDV
94
J558.66.165



 


Cluster014
 180
QVDYYGSSYWYFDV
95
J558.66.165



 


Cluster015
 179
LGYGSSYLYFDV
96
J558.66.165



 


Cluster016
 175
LGYGSIYLYFDV
97
J558.66.165



 


Cluster017
 168
LTDYGSGTYWFFDV
98
J558.66.165



 


Cluster018
 162
LDYYGSSYGWYFDV
99
J558.66.165



 


Cluster019
 162
YGDYINSVDY
100
J558.66.165



 


Cluster020
 159
YTDYINSVDY
101
J558.66.165



 


Cluster021
 156
LDTIVEDWYFDV
102
J558.66.165



 


Cluster022
 148
DYYGSSYGFDY
103
VGAM66.165
 1


 


Cluster023
 147
IYSNSLIMDY
104
J558.66.165



 


Cluster024
 143
LGYGSSYWYFDV
105
J558.66.165



 


Cluster025
 142
GGYYPYAMDY
106
J558.12.102
 1


 


Total 
7190


# different VH
 7












sample 1146












Name
Size
CDR3
SEQ ID NO:
VH
#/25





Cluster001
 2570
EGRGNYPFDY
107
36-60.6.70
 2


 


Cluster002
 1791
DYSYYAMDY
108
J559.12.162
 1


 


Cluster003
 1251
MRLYYGIDSSYWYFDV
109
3609.7.153
 3


 


Cluster004
  905
MRLFYGSRYSYWYFDV
110
3609.7.153



 


Cluster005
  841
SYYYGSRESDY
111
J558.53.146
 1


 


Cluster006
  614
GKYYPYYFDY
112
J558.12.102
 2


 


Cluster007
  515
WGSSGY
113
J558.55.149
 1


 


Cluster008
  477
TGYNNYGSRFIY
114
J558.18.108
 3


 


Cluster009
  441
RLVDY
115
J558.67.166
 3


 


Cluster010
  378
WWFLRGVYVMDY
116
J558.85.191
 4


 


Cluster011
  306
TGYNNYGSRFTY
117
J558.18.108



 


Cluster012
  303
RLVEY
91
J558.67.166



 


Cluster013
  291
RLIEY
118
J558.67.166



 


Cluster014
  291
GDWYFDV
119
VGAM.8-3-61
 2


 


Cluster015
  265
RQFLLGVYAMDY
120
J558.85.191



 


Cluster016
  237
RHFLLGVYAMDY
121
J558.85.191



 


Cluster017
  230
EGRVTTLDY
122
36-60.6.70



 


Cluster018
  216
GDWYFDY
123
VGAM.8-3-61



 


Cluster019
  212
MRLFYGSSYSYWYFDV
124
3609.7.153



 


Cluster020
  203
GSGYVYAMDY
125
VGAM3.8-4-71
 1


 


Cluster021
  200
GTTAYYAMDY
126
VGAM3.8-3-61
 2


 


Cluster022
  197
TGYNNYGSRFAY
127
J558.18.108



 


Cluster023
  182
GKYYPYYFVY
128
J558.12.102



 


Cluster024
  163
GTTSYYAMDY
129
VGAM3.8-3-61



 


Cluster025
  153
RGSYGTCFDY
130
J558.85.191



 


Total 
13232


# different VH 
12









The results from Table 10 show that different VH genes were amplified and sequenced in the PCR and deep sequencing procedures. Sample 1145 contains many clusters within the 25 largest clusters that use the J558.66.165 gene. Sample 1146 contains a large diversity of VH genes with 12 different VH genes in which each is present between one and four times within the 25 largest clusters. These results suggest that the method allows unbiased amplification and analysis of IgG VH repertoires.
To conclude, these experiments resulted in a ranking of the most frequent VHs that represent sequences used by B cells that have undergone activation and isotype switching as a result of stimulation by antigen. The VH region sequences present in the most frequently-occurring clusters derived from the transgenic mice containing the human IGVκ1-39 light chain can be used to combine with the sequence of human IGVκ1-39 light chain to collections of human monoclonal antibodies enriched for antibodies specific for EGFR.
Example 4
Immunization Strategies for the Construction of Reliable VH CDR3 Heat Maps
A broad array of Immunization methods is available that use various formats of antigen in combination with adjuvant to optimize the antigen-specific immune response in animals. For the ranking of frequently used heavy chain genes optimally representing VH regions from B cells that have expanded as a result of stimulation with the antigen of interest, it is critical that immunization protocols are used that focus the immune response on said antigen or even on an epitope of said antigen. Thus, the use of antigens fused or coupled to carrier proteins (such as Fc fusion proteins or proteins coupled to carriers like Keyhole Limpet Hemocyanine, known in the art) is to be avoided or restricted to a single step in the immunization procedure like a single primary immunization or a single booster immunization. It is expected that even limited activation of B cells through the use of carrier or fusion proteins or adjuvant may show up in ranked VH sequences/HCDR3 heat maps, thereby contaminating the analysis. Ideally, the immunizations are thus performed with ‘essentially pure antigens’. The present example demonstrates that single or repeated immunization with an antigen fused to an Fc-portion indeed results in expansion of irrelevant B cells, i.e. B cells that react with the Fc-portion rather than with the antigen of interest.
Single human VL transgenic mice (group 1) and wildtype mice (group 2) were immunized with the EGFR overexpressing tumor cell line A431 on days 0 and day 14 (2×10E6 A431 cells in 200 μl PBS), followed by ip immunization with Fc-EGFR fusion protein emulsified in Titermax Gold. At day 35, serum was collected and tested in ELISA for the presence of anti-Fc antibodies.
As control, a third group of mice comprising both single human VL transgenic mice and wildtype mice (group 3) were immunized with Fc-EGFR fusion protein only on days 0, 14, 28, 42 and 52. At day 56, serum was collected and tested in ELISA for the presence of anti-Fc antibodies.
The results (FIG. 3) show that even after a single immunization with Fc-EGFR, antibodies against the Fc portion are detected in groups 1 and 2. Although the levels of anti-Fc antibodies in these two groups were lower than the levels of anti-Fc as observed in group 3, the VH regions encoding these anti-Fc antibodies will be found in CDR3 heat maps, constructed as described in example 1. If so desired, such Fc-specific binders can either be circumvented by using essentially pure antigens such as in DNA tattoo or by elimination of Fc-specific binders from the population of B cells prior to VH sequence analysis.
Example 5
DNA Vaccination by Tattooing
DNA vaccination exploits plasmid DNA encoding a protein antigen to induce an immune response against said protein antigen. There is no need for purification of proteins for immunization and proteins, including membrane proteins, are expressed in their natural configuration on a cell membrane (Jechlinger et al., 2006/Quaak, et al., 2008/Stevenson et al., 2004).
In this example, we have used DNA tattoo vaccination, an invasive procedure involving a solid vibrating needle loaded with plasmid DNA that repeatedly punctures the skin, wounding both the epidermis and the upper dermis and causing cutaneous inflammation followed by healing (Bins 2005/Pokorna 2008). Here we used DNA tattoo vaccination strategies to induce antibody responses in mice. The goal was to assess the quality of the antibody response in the absence or presence of adjuvant. As described in example 4, for the construction of adequate CDR3 heat maps, it is desirable to focus the antibody response on the antigen of interest, omitting the use of adjuvant that causes clonal expansion of unwanted B cells.
For tattoo vaccination, plasmids encoding human ErbB2 and plasmids encoding influenza virus Hemagglutinin (HA) were used. Three DNA tattoo vaccination strategies were tested to optimize the priming and boosting of the immune response: (A) vaccination with vector DNA encoding the ErbB2 or HA antigen, (B) vaccination of vector DNA encoding ErbB2 or HA together with an adjuvant or (C) heterologous prime-boost vaccination with DNA encoding ERbB2 or HA followed by a boost with purified ErbB2 or HA protein in TM Gold adjuvant. In addition, control group (group D) mice were immunized with purified ErbB2 or HA in TM Gold adjuvant. To establish an optimized DNA tattoo vaccination protocol the following immunization protocols were used:
Group A (DNA Only): In this group, mice were vaccinated on day 0, 3, and 6 with plasmid DNA encoding ErbB2 or HA in the absence of adjuvant followed by a boost with the same DNA after four weeks. No adjuvant was used.
Group B (DNA+Genetic Adjuvant): To test if an adjuvant increases the priming of the humoral immune response, plasmid DNA encoding TANK-binding kinase 1 (TBK1) was co-vaccinated with ErbB2 or HA plasmid DNA. It has been shown that TBK1 acts as an adjuvant for DNA vaccination using a gene gun (Ishii et al., 2009). Comparison of group B with group A will reveal what impact the genetic adjuvant has on the generation of antibodies specific for HA or ErB2. Animals in group B were DNA vaccinated at same time points as those in group A. To examine the contribution of genetic adjuvant in priming of the immune system, plasmid DNA encoding TBK-1 was mixed in a 1:1 ratio with pVAX1-ErbB2 or pVAXI-HA and subsequently administrated by DNA tattoo. To this end mice are vaccinated with 20 μg pTBK-1 and 20 μg pVAX1-ErbB2 or pVAX1-HA in 10 μl PBS.
Group C (DNA+Protein): In this group a heterologous prime-boost protocol with DNA tattoo followed by intraperitoneal (i.p.) protein boost was tested to examine if a final protein boost is required to induce an antigen-specific serum IgG titer of > 1/1000 and if this boost is necessary to efficiently induce splenic memory B cells. I.p. injection is the direct immunization route to the spleen. So, by first priming the immune system by DNA vaccination ErbB2 or HA is presented to the immune system as in vivo expressed protein. Subsequently, the primed immune system was boosted with ErbB2 or HA in adjuvant via the i.p. injection route to induce a systemic immune reaction. Comparison of group C to A reveals the impact of the systemic boost on 1) antigen-specific IgG serum titer and 2) on generation of the splenic memory B cell compartment.
Immunization and first boost with pVAXI-ErbB2 or pVAX1-HA were carried out according to the scheme described for group A. Subsequently mice are boosted at day 28 and day 42 with 20 μg of protein in 200 μl emulsion of TitermaxGold adjuvant or in 200 μl PBS, respectively, administrated via ip injection. For HA vaccination mice were injected with 20 μg HA (Meridian life Science Inc, Cat no R01249). For ErbB2 mice were injected with 20 μg of a truncated ErbB2 protein: the extra cellular domain (ECD, aa23-652) of ErbB2 fused to FC-tail (R&D systems, Cat no 1129ER).
Group D (Protein):In this control group mice were vaccinated i.p. with ErbB2 or HA in adjuvant. Material from this group served as positive control for the analyses of the samples from groups A-C. At day 0, 14 and 28 mice were vaccinated with 20 μg of ErbB2 or HA in 200 μl emulsion of TitermaxGold adjuvant. For final boost, mice received 20 μg of ErbB2 or HA dissolved in 200 μl PBS.
Serum titer and affinity were determined after each boost by ELISA and FACS respectively using standard protocols (Middendorp et al., 2002). To study affinity maturation during the vaccination protocol, the relative affinity of the polyclonal antigen-specific-lgG serum was determined by ELISA. The efficacy and quality of memory B cell induction at the end of each vaccination strategy was examined by FACS in spleen and draining inguinal lymph node (iLN) as described (Middendorp et al., 2002).
All ErbB2 DNA immunized mice developed an anti-ErbB2 IgG serum titer >10,000 after two immunization rounds (FIG. 4A-C), indicating that two rounds of DNA immunization via tattoo are sufficient to induce a strong anti-ErbB2 antibody response. The results show protein immunized mice develop a strong anti-ERbB2 IgG serum titer at day 21 (FIG. 4D).
A third immunization round at day 28 with DNA (groups A1 and B1) resulted in a further increase of the anti-ErbB2 IgG serum titer at day 35 (seven days after third vaccination round) and day 45 (end point) (FIGS. 4A and 3B). In group B1 we found that co-administration of the ErbB2 expression vector (group A1) together with a DNA-adjuvant (the pBoost3 vector encoding the TBK1 protein), would boost the antibody response against ErbB2. Comparison of the anti-ErbB2 serum titer in time showed that the mice in the groups A1 and B1 developed a comparable anti-ErbB2 serum titer (FIG. 4E-G). This indicated that for the ErbB2 antigen co-administration of the pBoost3 vector failed to enhance the polyclonal anti-ErbB2 IgG serum titer. The mice in group C1 first received two immunization rounds (day 0 and 14) with DNA followed by a boost (at day 28) with ErbB2-Fc protein emulsified with TitermaxGold adjuvant. This protein boost at day 28 resulted in a strong increase of the anti-ErbB2 IgG serum titer at day 35 (after third immunization at day 28) and day 45 (end point) (FIG. 4C). At day 35 the serum titer of group C1 (DNA—protein) was higher compared to the DNA only vaccinated mice (groups A1 and B1) and marginal lower compared to protein only immunized mice (group D1). At day 45 the serum titers of the groups C1 (DNA and protein) and D1 (protein only) were comparable.
All mice that were immunized with the HA antigen via the four vaccination strategies developed a strong anti-HA IgG serum titer at day 21 (data not shown) and 35 (FIG. 5). The anti-HA IgG serum titer between the DNA (group A2) and DNA+adjuvant (group B2) strategies were comparable. Moreover, DNA vaccination followed by a boost with HA protein emulsified with Titermax Gold (group C2) or three times immunization with protein (group 02) gave higher serum titer than DNA only vaccination. In summary, the similarities and differences between the four mice groups vaccinated with HA antigen were comparable to the results observed for the ErbB2 vaccinated mice in terms of antigen specific IgG serum titer.
To further compare the vaccination strategies we compared the polyclonal anti-ErbB2 IgG serum based on relative affinity. The relative affinity was measured in day 45 sera samples, obtained three days after the final boost. The relative affinity was determined by ELISA by incubating at a fixed serum dilution on an ErbB2 antigen titration starting at 0.5 μg/ml. The selected fixed serum dilution was based on the serum dilution at which the sera reached the plateau using a fixed concentration of ErbB2 antigen in ELISA (0.5 μg/ml). The fixed serum dilution was 1:1,500 for groups A1 and B1, and 1:20,000 for the groups C1 and D1. To compare the individual groups we calculated and plotted the relative binding based on the reduction of absorbance versus the antigen dilution range. For each antigen concentration we calculated the relative binding, the OD of 0.5 μg/ml was set to 100%.
First we compared DNA vaccination with (group B1) and without DNA adjuvant (group A1) (FIG. 6A). No difference was observed in the relative binding between the groups that received DNA (group A1) or DNA+adjuvant (group B1). This indicated that the DNA adjuvant did not enhance the affinity of the polyclonal serum. In addition, to compare the contribution of priming of the immune response with DNA followed by a protein boost we compared the sera of DNA-protein (group C1) and protein (group D1) (FIG. 6B). The relative binding was significant higher for group C1 than for group D1 (p<0.001 at antigen concentrations 0.0625 and 0.0313 μg/ml). This suggested that the relative affinity of the polyclonal serum was on average higher for mice in group C1 than for mice in group D1.
Isolation and analysis of tissues from immunized mice: Total splenic and total inguinal lymph node fractions from ErbB2 and HA vaccinated mice were collected and saved in Trizol LS. The draining inguinal lymph node from the tattooed leg was isolated and saved in Trizol LS. In addition we enriched the splenic IgG B cell fraction by MACS from mice immunized using strategy C1 and C2. Finally we determined the fraction of splenic IgG+ B cells in all ErbB2 and HA vaccinated mice. To isolate the splenic IgG+ B cells we performed a two step MACS purification. In the first step we depleted the non-B cell using biotinylated non-B cell specific antibodies. In the second step we enriched the splenic IgG B cells using anti-lgG1 and anti-lgG2ab specific antibodies. Table 11 gives an overview from which mice the splenic IgG (IgG1 and IgG2ab) B cells were isolated. Purity of the isolated IgG fractions was determined by FACS using B cell specific and IgG1/IgG2ab specific antibodies. The % Ig B cells was determined by staining a fraction of cells alter the depletion step. Table 11 summarizes the yield and purity of the isolated IgG+ B cell fractions per mouse and antigen used for immunization.







TABLE 11







yield and purity of the isolated IgG+ B cell fractions















% B




Strategy and


cells/
% IgG B
Total IgG


experimental

Animal
total life
cells/B cell
cells


group
Antigen
number
gate
gate
(E+0.6)















DNA and protein
ErbB2
31
68.46
30.27
1.36


DNA and protein
ErbB2
32
67.37
39.96
1.76


DNA and protein
ErbB2
33
20.78
61.75
3.63


DNA and protein
ErbB2
34
72.97
73.67
1.17


DNA and protein
ErbB2
35
86.67
77.34
ND


DNA and protein
ErbB2
36
84.98
82.12
ND


DNA and protein
ErbB2
37
85.03
74.32
ND


DNA and protein
HA
38
90.41
55.48
2.09


DNA and protein
HA
40
92.60
49.09
1.17


DNA and protein
HA
41
86.03
47.02
0.84


DNA and protein
HA
42
88.46
46.99
1.21









To examine what protocol gives the best induction of the memory B cell compartment, we examined the size of IgG B cell compartment in the spleen and the iLN per each vaccination strategy by FACS. We used a cocktail of anti-lgG1-FITC and anti-lgG2ab-FITC monoclonals to visualize the IgG B cell fraction. Table 12 gives an overview of the percentages B cells within the lymphocyte gate and the percentages IgG B cells within the B cell fraction per mouse and tissue.







TABLE 12







IgG+ B cell fraction in the spleen and lymph node of ErbB2


vaccinated mice










Spleen
iLN














% B
% IgG+ B
% B
% IgG+ B




cells/
cells/
cells/
cells/



Animal
lympho.
lympho.
lympho.
lympho.


Group
number
gate
gate
gate
gate















DNA (A1)
1
57.50
1.27
58.26
1.86


DNA (A1)
2
51.81
1.09
51.09
2.80


DNA (A1)
3
54.05
1.32
58.93
2.43


DNA (A1)
4
54.06
1.73
55.24
2.93


DNA (A1)
5
54.93
2.72
53.78
6.45


DNA (A1)
6
58.68
1.98
53.89
2.90


DNA (A1)
7
56.46
5.92
52.01
10.74


DNA + Adju (B1)
17
49.05
4.33
51.04
7.02


DNA + Adju (B1)
17
55.84
1.55
53.80
4.38


DNA + Adju (B1)
18
44.13
4.05
54.78
6.31


DNA + Adju (B1)
19
34.99
5.56
53.39
15.47


DNA + Adju (B1)
20
51.81
1.41
60.30
2.40


DNA + Adju (B1)
21
54.13
1.21
56.35
2.84


DNA + Adju (B1)
22
58.34
2.11
62.29
1.80


DNA + protein
31
48.99
6.26
54.12
6.49


(C1)







DNA + protein
32
49.76
7.38
51.79
7.67


(C1)







DNA + protein
33
45.94
12.76
45.14
4.19


(C1)







DNA + protein
34
30.50
16.07
44.83
10.91


(C1)







DNA + protein
35
43.48
26.62
44.60
8.16


(C1)







DNA + protein
36
41.12
24.07
45.13
11.00


(C1)







DNA + protein
37
30.50
21.10
30.82
9.17


(C1)









The mice from group C1 (DNA-protein) that received a correct i.p. boost with ErbB2-Fc protein at day 28 (mice 35-37) had the largest fraction of IgG+ B cells per splenic B cell population. This was expected as i.p. injection is the direct route of the antigen to the spleen. The percentages of IgG+ B cells in the iLN of group A1 (DNA) and B1 (DNA+adjuvant) ranged between 1.8-15.47 with an averaged IgG+ B cell fraction of 4.3 and 5.7 for group A1 or B1, respectively. Interestingly, the mice from group A1 and B1 with the highest fraction of IgG+ B cells in the iLN also contained a higher percentage of splenic IgG+ B cells. Analysis of the percentages of IgG B cells in the iLN of the HA vaccinated mice showed that these mice had on average comparable percentages of IgG+ B cells per all iLN B cells as found in the A1 and B1 groups. The averaged percentages of IgG+ B cells in the iLN of DNA vaccinated (group A2) and the DNA+adjuvant vaccinated (group B2) mice was 5.0 and 3.0, respectively. FIG. 7 gives a comparison of the percentages IgG B cells/iLN B cells per vaccination strategy and per antigen. In summary, these data showed that the draining iLN of DNA only (group A) of DNA+adjuvant (group B) vaccinated mice had a significant fraction of IgG+ B cells. Mice that received a protein boost (group C) contained a larger IgG+ B cell population in spleen and iLN.
We conclude that mice that were vaccinated with DNA or vaccinated with DNA and boosted with protein developed a strong antigen specific IgG serum titer. The relative affinity of the sera against ErbB2 can be significantly enhanced using a DNA+protein immunization protocol instead of a protein immunization protocol. The small variation of the antigen IgG serum titer between individual DNA vaccinated mice shows that the DNA tattoo method has been carried out consistently. DNA vaccination with ERbB2and HA both resulted in strong antigen specific antibody response.
It was reported that the adjuvant effect of plasmid DNA is mediated by its double-stranded structure, which activates Tbk1-dependent innate immune signaling pathways in the absence of HRs (Ishii et al., 2008). Therefore, co-administration of a Tbk1-expressing plasmid was expected to further boost DNA vaccine-induced immunogenicity. In our setting, we did not observe a beneficial effect of the co-administration of the Tbk1 encoding pBoost3 vector. Co-administration of pBoost3 together with the antigen encoding vector failed to result in a higher serum titer, increased relative affinity or enhanced IgG B cell formation.
FACS analysis showed that the draining i1N in a mouse vaccinated with only DNA (group A and B) contains a significant IgG+ B cell fraction. However the number of IgG B cells that can be isolated from a single draining i1N is very limited due to the size of an i1N. Moreover, the fraction of IgG+ B cells in the i1N varied significantly between individual DNA vaccinated mice. This could be the results of variation in administration of DNA via DNA tattoo. Interestingly, the mice with a high percentage of i1N IgG+ B cells also had a higher percentage of splenic IgG+ B cells. Another strategy to obtain a larger number of IgG+ B cells is to boost the DNA vaccinated mice once with protein emulsified in TitermaxGold. The mice that were vaccinated with DNA and protein (group C) developed a significant splenic IgG+ B cell fraction (in addition to a large i1N IgG+ B cell fraction). If no protein is available to for boost immunizations, mice can be boosted with cells expressing the antigen or virus-like particles expressing the antigen.
In conclusion, DNA vaccination via DNA tattooing is an effective and robust vaccination strategy to induce an antigen specific humoral immune response. The mice that were vaccinated with only DNA (group A) developed a detectable IgG+ B cell fraction.
Example 6
Construction of Eukaryotic Vectors for the Efficient Production of Single VL Bispecific Human Monoclonal Antibodies
One aspect of the present invention concerns the possibility of using sequence information from VH gene frequency analysis and/or HCDR3 heat maps to generate panels of antibodies in a desirable therapeutic format and screen those antibodies for binding and or functional activity. One such format is a bispecific IgG molecule. Conventional IgG molecules are comprised of two identical heavy- and two identical light chains. Heavy chains are polypeptides made up from separate domains: a VH region for antigen recognition, the CH1 domain, the hinge region, the CH2 domain and the CH3 domain. Pairing of the heavy chains to form a homodimer is the result of high affinity interactions between the CH3 domains, where after covalent coupling of the two heavy chains results from disulfide bridge formation between cysteines in the hinge region of the heavy chains.
The CH3 region has been used to introduce amino acid substitutions that inhibit the formation of homodimers (pairing of heavy chains with an identical CH3 region) while promoting heterodimerization (pairing of 2 different heavy chains with complementary, engineered CH3 regions). This has resulted in efficient heterodimer formation upon co-expression of CH3 engineered heavy chains (Gunasekaran et al., 2010; WO2006/106905; WO2009/089004).
In this example, we have constructed and tested expression vectors for the efficient production of human bispecific single VL antibodies. The overall strategy is that genetic constructs encoding 2 different antibodies are co-transfected into a single cell. By using complementary engineered CH3 regions for the 2 different heavy chains, formation of heterodimers (bispecific antibodies) is favored over the formation of homodimers (monospecific antibodies). Previously, it was shown that the combination of K409D:K392D in the CH3 domain of one heavy chain in combination with D399′K:E356′K in the CH3 domain of the second chain (thus, so-called complementary engineered CH3 regions) drives the heterodimerization of human heavy chain constant regions in an engineered bispecific molecule (Gunasekaran 2010). We used to the same amino acid pairs in the CH3 regions of constructs encoding single VL IgG antibodies to establish whether this would result in the efficient production of bispecific single VL human IgG monoclonal antibodies through heterodimerization.
The rearranged single IGVκ1-39 VL gene was cloned into a eukaryotic expression vector that contains the human gammal and kappa constant regions essentially as described (Throsby et al., 2008/de Kruif et al., 2009). Vector MV1201 contains DNA encoding a CH3 domain with the K409D:K392D amino acid substitutions in combination with a VH region encoding a human single VL monoclonal antibody specific for fibrinogen. Vector MV1200 contains DNA encoding a CH3 domain with the D399′K:E356′K amino acid substitutions in combination with a VH region encoding a human single VL monoclonal antibody specific for thyroblobulin (Gunasekaran et al., 2010/de Kruif et al., 2009).
MV1201 and MV 1200 were co-transfected into HEK293T cells and transiently expressed as described (de Kruif et al., 2009). After 13 days, supernatants were harvested and purified by protein A affinity chromatography using standard protocols. The protein A-purified IgG was analyzed by SDS-PAGE under reduced and non-reducing conditions; staining of proteins in the gel was carried out with colloidal blue. The results of this experiment are shown in FIG. 8 Under non-reducing SDS-PAGE, a single band with molecular weight of 150 kD was detected, showing that with these constructs hetero- and or homodimers were formed and no IgG half molecules consisting of a non-paired heavy/light chain combination.
After protein A purification, mass spectrometry was used to identify the different IgG species produced by the transiently transfected cells. As shown in FIG. 9, the supernatant contained 96% heterodimeric IgG and 3% and 1% of each of the parental monoclonal antibodies. Thus, these protein A supernatants can be immediately used for screening of binding activity and/or functional assays of bispecific antibodies.
Example 7
Generation of Bispecific Antibodies Specific for ErbB1 and ErbB2 Tumor Antigens and Analysis of Tumor Cell Killing and Inhibition of Tumor Cell Proliferation
Erbb1 and ErbB2 are growth factor receptors that play an important role in tumor development and progression. Combinations of monoclonal antibodies against ErbB1 and ErbB2 have shown synergistic effects in animal models of cancer (Larbouret et al., 2007) and therefore represent promising therapeutics for the treatment of cancer in humans. In this example, we demonstrate that, upon immunization, human monoclonal antibodies can be obtained from transgenic mice with a single human light chain through high throughput sequencing and creation of CDR3 heat maps and that combinations of ErbB1 and ErbB2 antibodies with additive and/or synergistic effect can be rapidly identified by in vitro screening.
Transgenic mice with a single human light chain are immunized with ErbB2 DNA and protein as described in example 5. Using the same protocols, another group of mice is immunized with ErbB1 DNA and proteins using the same procedures. For ErbB1 and ErbB2 immunized mice, spleens are isolated and the VH repertoire of IgG+ B cells is analyzed by high throughput sequencing as described in examples 1 and 2. After construction of CDR3 heat maps, the top 100 VH sequence groups for each ErbB1 and ErbB2 immunized mice are selected for further analysis. For the collection of ErbB1 and ErbB2 VH sequences, VH sequences representative for each cluster, this is the VH gene that is present most frequently within a cluster, which may be a germline gene or a gene containing mutations, are cloned in an expression vector containing the single light chain and the CH3 mutation; ErbB1 VHs in MV1200 and ErbB2 VHs in MV1201. HEK293 cells are transiently transfected with all 2500 combinations (50 times 50) of cloned ErbB1 and ErbB2 VH sequence groups using the expression constructs that drive heterodimerization to form ErbB1×ErbB2 bispecific antibodies as described in example 6. After 13 days, culture supernatants are harvested and purified using protein A affinity chromatography. Purified IgG is used in functional assays of tumor cell killing and inhibition of tumor cell proliferation known in the art.
After identification of ErbB1×ErbB2 bispecific antibodies with potent anti-tumor activity, VH sequence collections present in the clusters that have an identical or similar CDR3 and that are used in the functional bispecific antibodies can be further deconvoluted using the same approach to find those VH members in the collection that give the most potent anti-tumor activity.
Example 8
Deep Sequencing and Diversity Analysis of HCDR3 from Samples Obtained from Splenic B Cells from Non-Immunized Versus Immunized Mice
To demonstrate that the selective expansion of clones identified by unique HCDR3 sequences upon immunization can be analyzed by deep sequencing, splenic B cells from non-immunized and immunized mice were submitted to enrichment for B cells as described above. Nucleic acids were isolated and, where needed amplified as described above and cDNA was sent to Eurofins for high throughput sequencing.
Mice transgenic for huVκ1-39 and for a human heavy chain (HC) minilocus were immunized with protein only (fused to Fc) or by using alternating protein and cellular immunizations (with cells expressing the same antigen on their surface). cMet and EGFR were used as antigens in this example (two animals per group):

    
    
        Group A: cMet-Fc in Titermax Gold adjuvant (TMG) on days 0, 14 and 28, and cMet-Fc in PBS on day 47.
        Group B: cMet-Fc in TMG on days 0, 14 and 28, MKN45 cells in PBS on day 49, and cMet-Fc in PBS on day 64.
        Group C: EGFR-Fc in TMG on days 0, 14 and 28, and EGFR-Fc in PBS on day 54.
        Group D: EGFR-Fc in TMG on days 0, 14 and 28, A431 cells in PBS on day 49, and EGFR-Fc in PBS on day 64.
    
    


Doses used were 20 μg protein in 125 μl TMG, 20 μg protein in 200 μl PBS and 2×10E6 cells in 200 μl PBS. Non-immunized transgenic mice were around the same age as immunized mice at sacrifice (16 weeks, three mice in total).
Spleens were collected from all mice (for immunized mice three days after the last immunization) and processed as described in Example 2. To be able to sequence many different samples in a mixture at reduced cost, a material identification (MID) tag specific for each mouse was added at the 5′ end of each primer used in PCR amplification of cDNA together with the SMART sequence (as detailed in Example 2). MID tag addition thus allowed pooling of material from several mice after PCR amplification and before 454-sequencing. Primers for amplification were thus adjusted to include MID tag complementary sequences (Table 3).
For non-immunized mice, spleen cell suspensions were enriched for B-cells using anti-CD19 Microbeads (Miltenyi Biotec, cat. no. 130-052-201) and then sorted by flow cytometry to isolate mature, antigen-naive B cells expressing IgM or IgD. This was done by sorting for CD19-positive, B220-positive, huVκ1-39-positive and mouse light chain-negative B-cells and discriminating these in IgM-positive or IgD-positive cell fractions. In reverse primers used for cDNA synthesis, sequences were used that annealed to either IgM or IgD coding sequences. To be able to perform pooled sequencing for different samples, MID tags were used here to identify material from different samples (Table 14).







TABLE 13







Primers used for deep sequencing material from immunized mice


(one MID tag per mouse).











MID


Primer
Primer


tag
Sequence*
SEQ ID NO:
type
name














MID-01
ACGAGTGCGTAAGCAGTGGTATCAACGCAGAGT
131
Forward
SMART-






MID1-fw


 


MID-01
ACGAGTGCGTCAGGGGCCAGTGGATAGAC
132
Reverse
mIgG-CH1-






MID1-rev


 


MID-02
ACGCTCGACAAAGCAGTGGTATCAACGCAGAGT
133
Forward
SMART-






MID2-fw


 


MID-02
ACGCTCGACACAGGGGCCAGTGGATAGAC
134
Reverse
mIgG-CH1-






MID2-rev


 


MID-03
AGACGCACTCAAGCAGTGGTATCAACGCAGAGT
135
Forward
SMART-






MID3-fw


 


MID-03
AGACGCACTCCAGGGGCCAGTGGATAGAC
136
Reverse
mIgG-CH1-






MID3-rev


 


MID-04
AGCACTGTAGAAGCAGTGGTATCAACGCAGAGT
137
Forward
SMART-






MID4-fw


 


MID-04
AGCACTGTAGCAGGGGCCAGTGGATAGAC
138
Reverse
mIgG-CH1-






MID4-rev


 


MID-05
ATCAGACACGAAGCAGTGGTATCAACGCAGAGT
139
Forward
SMART-






MID5-fw


 


MID-05
ATCAGACACGCAGGGGCCAGTGGATAGAC
140
Reverse
mIgG-CH1-






MID-rev


 


MID-06
ATATCGCGAGAAGCAGTGGTATCAACGCAGAGT
141
Forward
SMART-






MID6-fw


 


MID-06
ATATCGCGAGCAGGGGCCAGTGGATAGAC
142
Reverse
mIgG-CH1-






MID6-rev


 


MID-07
CGTGTCTCTAAAGCAGTGGTATCAACGCAGAGT
143
Forward
SMART-






MID7-fw


 


MID-07 
CGTGTCTCTACAGGGGCCAGTGGATAGAC
144
Reverse
mIgG-CH1-






MID7-rev


 


MID-08 
CTCGCGTGTCAAGCAGTGGTATCAACGCAGAGT
145
Forward
SMART-






MID7-fw


 


MID-08 
CTCGCGTGTCCAGGGGCCAGTGGATAGAC
146
Reverse
mIgG-CH1-






MID8-rev





*The MID tag sequence is underlined; the SMART sequence is in bold; the IgG constant HC sequence in regular type.













TABLE 14







Primers used for deep sequencing material from non-immunized mice


(one MID tag per cell population per mouse).












Cell
MID


Primer
Primer


fraction*
tag
Sequence**
SEQ ID NO:
type
name















IgMHIGH
MID-
AGCACTGTAGAAGCAGTGGTATCAACGCAGAGT
137
Forward
SMART-


B cells
04



MID4-fw


mouse 1







 


IgMHIGH
MID-
AGCACTGTAGGGCCACCAGATTCTTATCAGAC
147
Reverse
mouse


B cells
04



IgM-CH1-


mouse 1




MID4-rev


 


IgDHIGH
MID-
ATCAGACACGAAGCAGTGGTATCAACGCAGAGT
139
Forward
SMART-


B cells
05



MID5-fw


mouse 1







 


IgDHIGH
MID-
ATCAGACACGCAGTTCTGAGGCCAGCACAGTG
148
Reverse
mouse


B cells
05



IgD-CH1-


mouse 1




MID5-rev


 


IgMHIGH
MID-
TCTCTATGCGAAGCAGTGGTATCAACGCAGAGT
149
Forward
SMART-


B cells
10



MID10-fw


mouse 2







 


IgMHIGH
MID-
TCTCTATGCGGGCCACCAGATTCTTATCAGAC
150
Reverse
mouse


B cells
10



IgM-CH1-


mouse 2




MID10-rev


 


IgDHIGH
MID-
TGATACGTCTAAGCAGTGGTATCAACGCAGAGT
151
Forward
SMART-


B cells
11



M D11-fw


mouse 2







 


IgDHIGH
MID-
TGATACGTCTCAGTTCTGAGGCCAGCACAGTG
152
Reverse
mouse 


B cells
11



IgD-CH1-


mouse 2




MID11-rev


 


IgMHIGH
MID-
TCACGTACTAAAGCAGTGGTATCAACGCAGAGT
153
Forward
SMART-


B cells
16



MID16-fw


mouse 3







 


IgMHIGH
MID-
TCACGTACTAGGCCACCAGATTCTTATCAGAC
154
Reverse
mouse


B cells
16



IgM-CH1-


mouse 3




MID16-rev


 


IgDHIGH
MID-
CGTCTAGTACAAGCAGTGGTATCAACGCAGAGT
155
Forward
SMART-


B cells
17



MID17-fw


mouse 3







 


IgDHIGH
MID-
CGTCTAGTACCAGTTCTGAGGCCAGCACAGTG
156
Reverse
mouse


B cells
17



gD-CH1-


mouse 3




M 1D17-







rev





*B cells selected for expression of only huVκ1-39 LC.


**The MID tag sequence is underlined; the SMART sequence is in bold; the IgD or IgM constant sequences in regular type.






For analysis of the sequencing results, custom designed algorithms were used for VH gene identification and alignment of HCDR3 regions. Briefly, raw sequence data were imported into a dedicated computer program, which translated all nucleotide sequences into six potential protein reading frames, each of which was then submitted to the following sequential filter criteria to find correct and complete human VH genes:

    
    
        Sequences shorter than 75 amino acids were rejected as this was considered as the minimal length to positively identify VH genes.
        Sequences without two canonical cysteines were rejected as these were used to identify VH genes and reading frames.
        Frameworks 1 to 4 were searched for based on homology with VH genes in a database. If one or more of these frameworks were not found, the sequence was rejected.
        CDR1, 2 and 3 were identified based on the identified framework regions. The sequence was rejected when a stop codon was present in one or more of the CDRs.
    
    


Sequences that passed these criteria were classified as annotated VH sequences. All selected VH sequences were then submitted to another algorithm to group them into clusters with a 100% identical HCDR3.
These data resulted in annotated VH genes and these VH genes were clustered based on HCDR3 sequence. To analyze the selective expansion of HCDR3 regions in VH genes in immunized versus non-immunized mice, the results were tabulated and expressed as the ratio of annotated VH regions over clusters with identical HCDR3 regions (Table 15). For ease of interpretation, results for separate B cell fractions from non-immunized mice were pooled so that the ratio could analyzed for total mature, antigen-naïve B cells.







TABLE 15







Deep sequencing data from immunized versus non-immunized mice.












Analyzed
Annotated
Clusters with




B cell
VH
identical
Ratio


Immunization
population
regions
HCDR3
VH/clusters














None
Total mature
39,626
30,716
1.3



B cells*





None
Total mature
6,102
5,676
1.1



B cells*





None
Total mature
9,128
8,437
1.1



B cells*





cMet protein
Total B cells
34,327
2,757
12.5


cMet protein
Total B cells
90,049
4,511
20.0


cMet protein &
Total B cells
19,645
3,233
6.1


cells






cMet protein &
Total B cells
75,838
4,557
16.6


cells






EGFR protein
IgG+ B cells
46,924
4518
10.4


EGFR protein
IgG+ B cells
3,799
1201
3.2


EGFR protein &
Total B cells
60,979
3526
17.3


cells






EGFR protein &
Total B cells
43,631
2452
17.8


cells





*B cells expressing either IgM or IgD.






From table 15 it can readily be observed that the number of clusters with identical HCDR3 in non-immunized mice is in the order of the number of annotated VH genes, which is reflected in VH/cluster ratios near 1.0. This implies that in these mice there was no trigger for selective expansion of B cells that would carry VHs with certain HCDR3 regions, as would be expected in the absence of an immunogenic stimulus. In contrast, in immunized mice the ratio of VH regions over clusters with identical HCDR3 is, although variable between individual mice, much higher and VH/cluster ratios range from 3.2 to 20.0. Thus, in immunized mice a large fraction of HCDR3 sequences is present in high frequency in the repertoire probably as a result of clonal expansion of antigen-reactive B cells due to immunization of the mice. This indicates that the high frequency VH regions are likely the clones that are specific for the antigen of interest and suggests that mining these VH genes and expressing them as Fab or IgG together with the common light chain will likely render antibodies with specificity for the antigen. Data that this is indeed the case are shown in Example 9. Functionality of these antigen-specific antibodies can subsequently be tested in functional assays.
Example 9
Deep Sequencing of VH Repertoires and HCDR3 Heat Map Generation from Immunized Mice Expressing a Common Light Chain
As is clear from Example 8, a large fraction of HCDR3 sequences is present at a high frequency in the VH repertoire of immunized mice carrying a common light chain as opposed to non-immunized mice. In the present example, the repertoire of these most frequently used VH genes from immunized mice was mined to find antigen-specific heavy chain-derived binding domains which upon combination with the common light chain will render functional antibodies against the target of interest.
Material from mice carrying the huVκ1-39 transgene and a human HC minilocus upon immunization with cMet or EGFR (protein-Fc, or protein-Fc alternating with cells expressing the respective proteins at their surface; two mice per strategy so eight in total) was generated and processed as described in Example 8.
Deep sequencing and VH repertoire analysis including clustering based on HCDR3 identity revealed a total 287920 sequences which could be grouped into 813 unique clusters. All sequences in a cluster were derived from the same germline VH gene. Clusters that contained more than 50 VHs (with identical HCDR3 at the amino acid level but otherwise different VH regions) were collected into one database for repertoires from all eight mice. This was done since (limited) overlap of HCDR3 sequences was observed between the repertoires of the eight mice. VH sequences were then ranked on HCDR3 length and HCDR3 sequence identity. Next, HCDR3 sequences were further grouped based on the likelihood of a unique VDJ (i.e. if HCDR3 in different clusters contained <2 amino acids difference then they were considered part of the same cluster and were grouped together). This process was performed manually in an Excel worksheet but could be better performed on the basis of HCDR3 nucleotide sequence alignment. Tools to facilitate this are in development. This resulted in a total of 399 clusters with the same number of total sequences. The clusters were then aligned based on their size (i.e. total number of sequences in the cluster) and the top  ̃100 clusters for each target selected. This resulted in a total of 228 unique sequences: 134 from cMet-immunized mice and 94 from EGFR-immunized mice. Finally to select the nucleotide sequence to be recloned as an IgG from each cluster, the VH amino acid sequence alignment for each cluster was analyzed and the most frequent sequence in the cluster was chosen for recloning.
The corresponding nucleotide sequences were retrieved, modified to contain restriction sites for cloning into an expression vector and for removal of excessive restriction sites by silent mutation and then synthesized according to procedures known to the skilled person. Synthesized genes were cloned in bulk into a vector for expression in human IgG1 format including the huVκ1-39 common light chain. Of the rendered clones, 400 clones were picked by standard procedures and sequenced, and bulk cloning was repeated for missing or incorrect sequences until >90% of the 228-repertoire was retrieved. DNA was recovered and transiently transfected into an antibody production cell line. Methods used to modify sequences, synthesize DNA, clone, sequence and produce IgG by transfection are all known in the art.
IgG concentration of productions was determined using Octet technology (FortéBIO) according to the manufacturer's instructions for basic quantitation with a protein A sensor chip with regeneration (FortéBIO, cat. no. 18-0004/-5010/-5012/-5013). The 228 IgG were subsequently submitted to testing for antigen specific binding by ELISA at a concentration of 5 μg/ml. Of the tested sequences, 16 out of 110 (15%) were found to specifically bind to cMet and 6 out of 88 (7%) to EGFR. The other frequent clusters represent, for the large majority, antibodies that are directed to the Fc part of the antigen. It should be noted that the Fc-tail of the Fc-fusion proteins can be particularly immunogenic (Ling 1987, Immunology, vol 62, part 1, pp 1-6). Since these sequences were derived from material of mice immunized with Fc-fusion proteins in adjuvant, a large part of the humoral response will be directed to the Fc-tail (see Example 3 and 4). Binding to cells expressing the antigen on their surface was only done for IgG with binding domains derived from mice that were immunized with protein and cells, using cMet-expressing MKN45 cells or EGFR-expressing A431 cells. All IgG from cell-immunized mice that stained cMet or EGFR in ELISA also stained cells expressing the respective antigens (data not shown. It is expected that upon immunization with pure antigen such as via DNA tattoo, the percentage of antigen-specific clones will be further increased.
To functionally characterize the EGFR-specific IgG, these were tested for their potency to inhibit EGF-induced cell death of A431 cells (Gulli et al. 1996, Cell Growth Diff 7, p. 173-1′78). Of the 5 tested anti-EGFR mAbs, 3 were shown to inhibit EGF-induced A431 cell death (FIG. 10). The cMet-specific IgG were tested for functionality by determining their capacity to compete with several benchmark antibodies obtained from (patent) literature for binding to cMet in a binding competition ELISA. Briefly, 96-wells plates were coated with cMet-Fc and then incubated with an excess of one of several bench mark antibodies. Subsequently, tagged cMet-specific binders were added, followed by a peroxydase-labeled detection antibody that recognized bound cMet binders. The latter was detected using TMB as a substrate. Of the 10 tested cMet mAbs, 7 were demonstrated to compete for cMet binding with bench mark antibodies.
Thus, by using deep sequencing methods a broad panel of diverse antigen-specific VHs can be identified representing diverse VH usage, diverse HCDR3, and clonal maturation.
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## Claims

1. A method for producing a population of antibodies which bind to an antigen, said method comprising:
a) providing a population of B cells from a transgenic murine animal immunized with said antigen,
wherein said population of B cells comprises B cells obtained from one or more lymphoid organs of said immunized murine animal,
wherein said transgenic murine animal expresses a single rearranged human antibody light chain variable region (VL) from a VL nucleic acid,
wherein said transgenic murine animal expresses a human antibody heavy chain variable region (VH) repertoire from a repertoire of VH nucleic acids,
said B cell population expressing a repertoire of VHs and the single rearranged human antibody VL,
said B cell population thus expressing a limited immunoglobulin light chain variable (VL) region repertoire,
b) obtaining the nucleic acids encoding antibody heavy chain variable (VH) regions from essentially all of said B cells,
c) obtaining nucleotide sequences of the obtained nucleic acids of step b),
d) determining the frequency of nucleotide sequences from step c) which encode VH regions with unique HCDR3 amino acid sequences,
e) selecting nucleic acids encoding VH regions comprising frequently occurring HCDR3 amino acid sequences identified in step d),
f) providing host cells, wherein each host cell comprises at least one vector comprising a nucleotide sequence encoding at least one VH region selected in step e) wherein each said host cell expresses the single rearranged human VL nucleic acid of step a) and at least one VH region selected in step e),
g) culturing said host cells from step f) and allowing for expression of said VL and VH regions, and
h) obtaining said population of antibodies from the cultured host cells of step g) which bind said antigen.

2. The method of claim 1, further comprising amplifying nucleic acids encoding antibody heavy chain variable regions obtained in step b).

3. The method of claim 1, further comprising subjecting a sample of said cultured host cells of step h) to at least one functional assay to confirm binding to said antigen, and selecting at least one cell that produces an antibody which binds said antigen.

4. The method of claim 1, wherein the host cells of step g) further contain at least one second nucleic acid for expression of nucleotide sequence that encodes a second VH region that binds to a different antigen from that bound by the first VH region and said population of antibodies in step i) comprises bispecific antibodies.

5. The method of claim 4, wherein the second nucleic acid encoding the second VH region is identified by:
a) providing a population of B cells from a transgenic murine animal immunized with said different antigen,
wherein said population of B cells comprises B cells obtained from one or more lymphoid organs of said immunized murine animal,
wherein said transgenic murine animal expresses a single rearranged human antibody light chain variable region (VL) from a VL nucleic acid,
wherein said transgenic murine animal expresses a human antibody heavy chain variable region (VH) repertoire from a repertoire of VH nucleic acids,
said B cell population expressing a repertoire of VHs and the single rearranged human antibody VL,
said B cell population thus expressing a limited immunoglobulin light chain variable (VL) region repertoire,
b) obtaining the nucleic acids encoding antibody heavy chain variable (VH) regions from said B cells,
c) obtaining nucleotide sequences of the obtained nucleic acids of step b),
d) determining the frequency of nucleotide sequences from step c) which encode VH regions with unique HCDR3 amino acid sequences,
e) selecting nucleic acids encoding VH regions comprising frequently occurring HCDR3 amino acid sequences identified in step d).

6. The method of claim 1, wherein the transgenic mouse has been immunized with a nucleic acid encoding said antigen or with a protein form of said antigen.

7. The method of claim 5, wherein the transgenic mouse has been immunized with a nucleic acid encoding said antigen or with a protein form of said antigen.