# Patent

## Title
Nucleic acid agents for overexpressing or downregulating RNA interference targets and uses of same in improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of a plant

## Abstract

A method of improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of a plant is provided by expressing within the plant an exogenous polynucleotide encoding a polypeptide having an amino acid sequence at least 80% homologous to SEQ ID NOs: 687-981, 992-1248, 1281-1310, 1389-1391, and 2806-3081. Also provided is a method of improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of a plan by expressing within the plant an exogenous polynucleotide which downregulates an activity or expression of a polypeptide having an amino acid sequence at least 80% homologous to SEQ ID NOs: 311-514, 2007-2436, 1311-1320, 982-991, 1249-1280, 1321-1388. Transgenic plants and constructs are provided as well.

## Background
RELATED APPLICATIONS
This application is a National Phase of PCT Patent Application No. PCT/IB2012/054149 having International filing date of Aug. 14, 2012, which claims the benefit of priority under 35 USC § 119(e) of U.S. Provisional Patent Application No. 61/523,355 filed on Aug. 14, 2011. The contents of the above applications are all incorporated by reference as if fully set forth herein in their entirety.


SEQUENCE LISTING STATEMENT
The ASCII file, entitled 58604SequenceListing.txt, created on Feb. 13, 2014, comprising 9,493,532 bytes, submitted concurrently with the filing of this application is incorporated herein by reference.
FIELD AND BACKGROUND OF THE INVENTION
The present invention, in some embodiments thereof, relates to nucleic acid agents for overexpressing or downregulating RNA interference targets and uses of same in improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of a plant.
Plant growth is reliant on a number of basic factors: light, air, water, nutrients, and physical support. All these factors, with the exception of light, are controlled by soil to some extent, which integrates non-living substances (minerals, organic matter, gases and liquids) and living organisms (bacteria, fungi, insects, worms, etc.). The soil's volume is almost equally divided between solids and water/gases. An adequate nutrition in the form of natural as well as synthetic fertilizers, may affect crop yield and quality, and its response to stress factors such as disease and adverse weather. The great importance of fertilizers can best be appreciated when considering the direct increase in crop yields over the last 40 years, and the fact that they account for most of the overhead expense in agriculture. Sixteen natural nutrients are essential for plant growth, three of which, carbon, hydrogen and oxygen, are retrieved from air and water. The soil provides the remaining 13 nutrients.
Nutrients are naturally recycled within a self-sufficient environment, such as a rainforest. However, when grown in a commercial situation, plants consume nutrients for their growth and these nutrients need to be replenished in the system. Several nutrients are consumed by plants in large quantities and are referred to as macronutrients. Three macronutrients are considered the basic building blocks of plant growth, and are provided as main fertilizers; Nitrogen (N), Phosphate (P) and Potassium (K). Yet, only nitrogen needs to be replenished every year since plants only absorb approximately half of the nitrogen fertilizer applied. A proper balance of nutrients is crucial; when too much of an essential nutrient is available, it may become toxic to plant growth. Utilization efficiencies of macronutrients directly correlate with yield and general plant tolerance, and increasing them will benefit the plants themselves and the environment by decreasing seepage to ground water.
Nitrogen is responsible for biosynthesis of amino and nucleic acids, prosthetic groups, plant hormones, plant chemical defenses, etc, and thus is utterly essential for the plant. For this reason, plants store nitrogen throughout their developmental stages, in the specific case of corn during the period of grain germination, mostly in the leaves and stalk. However, due to the low nitrogen use efficiency (NUE) of the main crops (e.g., in the range of only 30-70%), nitrogen supply needs to be replenished at least twice during the growing season. This requirement for fertilizer refill may become the rate-limiting element in plant growth and increase fertilizer expenses for the farmer. Limited land resources combined with rapid population growth will inevitably lead to added increase in fertilizer use. In light of this prediction, advanced, biotechnology-based solutions to allow stable high yields with an added potential to reduce fertilizer costs are highly desirable. Subsequently, developing plants with increased NUE will lower fertilizer input in crop cultivation, and allow growth on lower-quality soils.
The major agricultural crops (corn, rice, wheat, canola and soybean) account for over half of total human caloric intake, giving their yield and quality vast importance. They can be consumed either directly (eating their seeds which are also used as a source of sugars, oils and metabolites), or indirectly (eating meat products raised on processed seeds or forage). Various factors may influence a crop's yield, including but not limited to, quantity and size of the plant organs, plant architecture, vigor (e.g. seedling), growth rate, root development, utilization of water and nutrients (e.g., nitrogen), and stress tolerance. Plant yield may be amplified through multiple approaches; (1) enhancement of innate traits (e.g., dry matter accumulation rate, cellulose/lignin composition), (2) improvement of structural features (e.g., stalk strength, meristem size, plant branching pattern), and (3) amplification of seed yield and quality (e.g., fertilization efficiency, seed development, seed filling or content of oil, starch or protein). Increasing plant yield through any of the above methods would ultimately have many applications in agriculture and additional fields such as in the biotechnology industry.
Two main adverse environmental conditions, malnutrition (nutrient deficiency) and drought, elicit a response in the plant that mainly affects root architecture (Jiang and Huang (2001), Crop Sci 41:1168-1173; Lopez-Bucio et al. (2003), Curr Opin Plant Biol, 6:280-287; Morgan and Condon (1986), Aust J Plant Physiol 13:523-532), causing activation of plant metabolic pathways to maximize water assimilation. Improvement of root architecture, i.e. making branched and longer roots, allows the plant to reach water and nutrient/fertilizer deposits located deeper in the soil by an increase in soil coverage. Root morphogenesis has already shown to increase tolerance to low phosphorus availability in soybean (Miller et al., (2003), Funct Plant Biol 30:973-985) and maize (Zhu and Lynch (2004), Funct Plant Biol 31:949-958). Thus, genes governing enhancement of root architecture may be used to improve NUE and drought tolerance. An example for a gene associated with root developmental changes is ANR1, a putative transcription factor with a role in nitrate (NO3−) signaling. When expression of ANR1 is down-regulated, the resulting transgenic lines are defective in their root response to localized supplies of nitrate (Zhang and Forde (1998), Science 270:407). Enhanced root system and/or increased storage capabilities, which are seen in responses to different environmental stresses, are strongly favorable at normal or optimal growing conditions as well.
Abiotic stress refers to a range of suboptimal conditions as water deficit or drought, extreme temperatures and salt levels, and high or low light levels. High or low nutrient level also falls into the category of abiotic stress. The response to any stress may involve both stress specific and common stress pathways (Pastori and Foyer (2002), Plant Physiol, 129: 460-468), and drains energy from the plant, eventually resulting in lowered yield. Thus, distinguishing between the genes activated in each pathway and subsequent manipulation of only specific relevant genes could lead to a partial stress response without the parallel loss in yield. Contrary to the complex polygenic nature of plant traits responsible for adaptations to adverse environmental stresses, information on miRNAs involved in these responses is very limited. The most common approach for crop and horticultural improvements is through cross breeding, which is relatively slow, inefficient, and limited in the degree of variability achieved because it can only manipulate the naturally existing genetic diversity. Taken together with the limited genetic resources (i.e., compatible plant species) for crop improvement, conventional breeding is evidently unfavorable. By creating a pool of genetically modified plants, one broadens the possibilities for producing crops with improved economic or horticultural traits.
SUMMARY OF THE INVENTION
According to an aspect of some embodiments of the present invention there is provided a method of improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of a plant, the method comprising expressing within the plant an exogenous polynucleotide encoding a polypeptide having an amino acid sequence at least 80% homologous to the polypeptide selected from the group consisting of SEQ ID NOs: 687-981, 992-1248, 1281-1310, 1389-1391, and 2806-3081, wherein the polypeptide is capable of regulating nitrogen use efficiency of the plant, thereby improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of the plant.
According to an aspect of some embodiments of the present invention there is provided a transgenic plant exogenously expressing a polynucleotide encoding a polypeptide having an amino acid sequence at least 80% homologous to the polypeptide selected from the group consisting of SEQ ID NOs: 687-981, 992-1248, 1281-1310, 1389-1391, and 2806-3081, wherein the polypeptide is capable of regulating nitrogen use efficiency of the plant.
According to an aspect of some embodiments of the present invention there is provided a nucleic acid construct comprising a polynucleotide encoding a polypeptide having an amino acid sequence at least 80% homologous to SEQ ID NOs:687-981, 992-1248, 1281-1310, 1389-1391, and 2806-3081, wherein the polypeptide is capable of regulating nitrogen use efficiency of the plant, and wherein the polynucleotide is under a transcriptional control of a cis-acting regulatory element.
According to an aspect of some embodiments of the present invention there is provided a method of improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of a plant, the method comprising expressing within the plant an exogenous polynucleotide which downregulates an activity or expression of a polypeptide having an amino acid sequence at least 80% homologous to SEQ ID NOs: 311-514, 2007-2436, 1311-1320, 982-991, 1249-1280, 1321-1388, wherein the polypeptide is capable of regulating nitrogen use efficiency of the plant, thereby improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of the plant.
According to an aspect of some embodiments of the present invention there is provided a transgenic plant exogenously expressing a polynucleotide which downregulates an activity or expression of a polypeptide having an amino acid sequence at least 80% homologous to SEQ ID NOs: 311-514, 2007-2436, 1311-1320, 982-991, 1249-1280, 1321-1388, wherein the polypeptide is capable of regulating nitrogen use efficiency of the plant.
According to an aspect of some embodiments of the present invention there is provided a nucleic acid construct comprising a polynucleotide which downregulates an activity or expression of a polypeptide having an amino acid sequence at least 80% homologous to SEQ ID NOs: 311-514, 2007-2436, 1311-1320, 982-991, 1249-1280, 1321-1388, wherein the polypeptide is capable of regulating nitrogen use efficiency of a plant, the nucleic acid sequence being under the regulation of a cis-acting regulatory element.
According to some embodiments of the invention, the polynucleotide is selected from the group consisting of SEQ ID NO: 1392-1646, 1655-1888, 1911-1938, 3082-3316.
According to some embodiments of the invention, the polypeptide is selected from the group consisting of SEQ ID NO: 687-981, 992-1248, 1281-1310, 1389-1391, and 2806-3081.
According to some embodiments of the invention, the cis-acting regulatory element comprises a promoter.
According to some embodiments of the invention, the promoter comprises a tissue-specific promoter.
According to some embodiments of the invention, the tissue-specific promoter comprises a root specific promoter.
According to some embodiments of the invention, the method further comprising growing the plant under limiting nitrogen conditions.
According to some embodiments of the invention, the method further comprising growing the plant under abiotic stress.
According to some embodiments of the invention, the abiotic stress is selected from the group consisting of salinity, drought, water deprivation, flood, etiolation, low temperature, high temperature, heavy metal toxicity, anaerobiosis, nutrient deficiency, nutrient excess, atmospheric pollution and UV irradiation.
According to some embodiments of the invention, the plant of some embodiments of the invention being a monocotyledon.
According to some embodiments of the invention, the plant of some embodiments of the invention being a dicotyledon.
According to some embodiments of the invention, the polynucleotide acts by a mechanism selected from the group consisting of sense suppression, antisense suppression, ribozyme inhibition, and gene disruption.
Unless otherwise defined, all technical and/or scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the invention pertains. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of embodiments of the invention, exemplary methods and/or materials are described below. In case of conflict, the patent specification, including definitions, will control. In addition, the materials, methods, and examples are illustrative only and are not intended to be necessarily limiting.



BRIEF DESCRIPTION OF THE DRAWINGS
Some embodiments of the invention are herein described, by way of example only, with reference to the accompanying drawings. With specific reference now to the drawings in detail, it is stressed that the particulars shown are by way of example and for purposes of illustrative discussion of embodiments of the invention. In this regard, the description taken with the drawings makes apparent to those skilled in the art how embodiments of the invention may be practiced.
In the drawings:
FIG. 1 is a scheme of a binary vector that can be used according to some embodiments of the invention.



DESCRIPTION OF SPECIFIC EMBODIMENTS OF THE INVENTION
The present invention, in some embodiments thereof, relates to nucleic acid agents for overexpressing or downregulating RNA interference targets and uses of same in improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of a plant.
Before explaining at least one embodiment of the invention in detail, it is to be understood that the invention is not necessarily limited in its application to the details set forth in the following description or exemplified by the Examples. The invention is capable of other embodiments or of being practiced or carried out in various ways.
The doubling of agricultural food production worldwide over the past four decades has been associated with a 7-fold increase in the use of nitrogen (N) fertilizers. As a consequence, both the recent and future intensification of the use of nitrogen fertilizers in agriculture already has and will continue to have major detrimental impacts on the diversity and functioning of the non-agricultural neighbouring bacterial, animal, and plant ecosystems. The most typical examples of such an impact are the eutrophication of freshwater and marine ecosystems as a result of leaching when high rates of nitrogen fertilizers are applied to agricultural fields. In addition, there can be gaseous emission of nitrogen oxides reacting with the stratospheric ozone and the emission of toxic ammonia into the atmosphere. Furthermore, farmers are facing increasing economic pressures with the rising fossil fuels costs required for production of nitrogen fertilizers.
It is therefore of major importance to identify the critical steps controlling plant nitrogen use efficiency (NUE). Such studies can be harnessed towards generating new energy crop species that have a larger capacity to produce biomass with the minimal amount of nitrogen fertilizer.
While reducing the present invention to practice, the present inventors have uncovered dsRNA sequences that are differentially expressed in maize plants grown under nitrogen limiting conditions versus corn plants grown under conditions wherein nitrogen is a non-limiting factor. Following further screening the present inventors were able to identify the target genes of these dsRNA sequences and suggest using same or sequences modulating expression of same in the generation of transgenic plants having improved nitrogen use efficiency.
According to some embodiments, the target genes or nucleic acid sequences controlling expression of same relay their effect by affecting at least one of:
root architecture so as to increase nutrient uptake;
activation of plant metabolic pathways so as to maximize nitrogen absorption or localization; or alternatively or additionally
modulating plant surface permeability.
Each of the above mechanisms may affect water uptake as well as salt absorption and therefore embodiments of the invention further relate to enhancement of abiotic stress tolerance, biomass, vigor or yield of the plant.
Thus, according to an aspect of the invention there is provided a method of improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of a plant, the method comprising expressing within the plant an exogenous polynucleotide encoding a polypeptide having an amino acid sequence at least 80% homologous to SEQ ID NOs: 687-981, 992-1248, 1281-1310, 1389-1391, and 2806-3081—wherein the polypeptide is capable of regulating nitrogen use efficiency of the plant, thereby improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of the plant.
As used herein the phrase “nitrogen use efficiency (NUE)” refers to a measure of crop production per unit of nitrogen fertilizer input. Fertilizer use efficiency (FUE) is a measure of NUE. Crop production can be measured by biomass, vigor or yield. The plant's nitrogen use efficiency is typically a result of an alteration in at least one of the uptake, spread, absorbance, accumulation, relocation (within the plant) and use of nitrogen absorbed by the plant. Improved NUE is with respect to that of a non-transgenic plant (i.e., lacking the transgene of the transgenic plant) of the same species and of the same developmental stage and grown under the same conditions.
As used herein the phrase “nitrogen-limiting conditions” refers to growth conditions which include a level (e.g., concentration) of nitrogen (e.g., ammonium or nitrate) applied, which is below the level needed for optimal plant metabolism, growth, reproduction and/or viability.
The phrase “abiotic stress” as used herein refers to any adverse effect on metabolism, growth, viability and/or reproduction of a plant. Abiotic stress can be induced by any of suboptimal environmental growth conditions such as, for example, water deficit or drought, flooding, freezing, low or high temperature, strong winds, heavy metal toxicity, anaerobiosis, high or low nutrient levels (e.g. nutrient deficiency), high or low salt levels (e.g. salinity), atmospheric pollution, high or low light intensities (e.g. insufficient light) or UV irradiation. Abiotic stress may be a short term effect (e.g. acute effect, e.g. lasting for about a week) or alternatively may be persistent (e.g. chronic effect, e.g. lasting for example 10 days or more). The present invention contemplates situations in which there is a single abiotic stress condition or alternatively situations in which two or more abiotic stresses occur.
According to an exemplary embodiment the abiotic stress refers to salinity.
According to another exemplary embodiment the abiotic stress refers to drought.
As used herein the phrase “abiotic stress tolerance” refers to the ability of a plant to endure an abiotic stress without exhibiting substantial physiological or physical damage (e.g. alteration in metabolism, growth, viability and/or reproductivity of the plant).
As used herein the term/phrase “biomass”, “biomass of a plant” or “plant biomass” refers to the amount (e.g., measured in grams of air-dry tissue) of a tissue produced from the plant in a growing season. An increase in plant biomass can be in the whole plant or in parts thereof such as aboveground (e.g. harvestable) parts, vegetative biomass, roots and/or seeds.
As used herein the term/phrase “vigor”, “vigor of a plant” or “plant vigor” refers to the amount (e.g., measured by weight) of tissue produced by the plant in a given time. Increased vigor could determine or affect the plant yield or the yield per growing time or growing area. In addition, early vigor (e.g. seed and/or seedling) results in improved field stand.
As used herein the term/phrase “yield”, “yield of a plant” or “plant yield” refers to the amount (e.g., as determined by weight or size) or quantity (e.g., numbers) of tissues or organs produced per plant or per growing season. Increased yield of a plant can affect the economic benefit one can obtain from the plant in a certain growing area and/or growing time.
According to an exemplary embodiment the yield is measured by cellulose content.
According to another exemplary embodiment the yield is measured by oil content.
According to another exemplary embodiment the yield is measured by protein content.
According to another exemplary embodiment, the yield is measured by seed number per plant or part thereof (e.g., kernel).
A plant yield can be affected by various parameters including, but not limited to, plant biomass; plant vigor; plant growth rate; seed yield; seed or grain quantity; seed or grain quality; oil yield; content of oil, starch and/or protein in harvested organs (e.g., seeds or vegetative parts of the plant); number of flowers (e.g. florets) per panicle (e.g. expressed as a ratio of number of filled seeds over number of primary panicles); harvest index; number of plants grown per area; number and size of harvested organs per plant and per area; number of plants per growing area (e.g. density); number of harvested organs in field; total leaf area; carbon assimilation and carbon partitioning (e.g. the distribution/allocation of carbon within the plant); resistance to shade; number of harvestable organs (e.g. seeds), seeds per pod, weight per seed; and modified architecture [such as increase stalk diameter, thickness or improvement of physical properties (e.g. elasticity)].
As used herein the term “improving” or “increasing” refers to at least about 2%, at least about 3%, at least about 4%, at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90% or greater increase in NUE, in tolerance to abiotic stress, in yield, in biomass or in vigor of a plant, as compared to a native or wild-type plants [i.e., plants not genetically modified to express the biomolecules (polynucleotides or polypeptides) of the invention, e.g., a non-transformed plant of the same species and of the same developmental stage which is grown under the same growth conditions as the transformed plant].
Improved plant NUE is translated in the field into either harvesting similar quantities of yield, while implementing less fertilizers, or increased yields gained by implementing the same levels of fertilizers. Thus, improved NUE or FUE has a direct effect on plant yield in the field.
The term “plant” as used herein encompasses whole plants, ancestors and progeny of the plants and plant parts, including seeds, shoots, stems, roots (including tubers), and isolated plant cells, tissues and organs. The plant may be in any form including suspension cultures, embryos, meristematic regions, callus tissue, leaves, gametophytes, sporophytes, pollen, and microspores.
As used herein the phrase “plant cell” refers to plant cells which are derived and isolated from disintegrated plant cell tissue or plant cell cultures.
As used herein the phrase “plant cell culture” refers to any type of native (naturally occurring) plant cells, plant cell lines and genetically modified plant cells, which are not assembled to form a complete plant, such that at least one biological structure of a plant is not present. Optionally, the plant cell culture of this aspect of the present invention may comprise a particular type of a plant cell or a plurality of different types of plant cells. It should be noted that optionally plant cultures featuring a particular type of plant cell may be originally derived from a plurality of different types of such plant cells.
Any commercially or scientifically valuable plant is envisaged in accordance with these embodiments of the invention. Plants that are particularly useful in the methods of the invention include all plants which belong to the super family Viridiplantae, in particular monocotyledonous and dicotyledonous plants including a fodder or forage legume, ornamental plant, food crop, tree, or shrub selected from the list comprising Acacia spp., Acer spp., Actinidia spp., Aesculus spp., Agathis australis, Albizia amara, Alsophila tricolor, Andropogon spp., Arachis spp, Areca catechu, Astelia fragrans, Astragalus cicer, Baikiaea plurijuga, Betula spp., Brassica spp., Bruguiera gymnorrhiza, Burkea africana, Butea frondosa, Cadaba farinosa, Calliandra spp, Camellia sinensis, Canna indica, Capsicum spp., Cassia spp., Centroema pubescens, Chacoomeles spp., Cinnamomum cassia, Coffea arabica, Colophospermum mopane, Coronillia varia, Cotoneaster serotina, Crataegus spp., Cucumis spp., Cupressus spp., Cyathea dealbata, Cydonia oblonga, Cryptomeria japonica, Cymbopogon spp., Cynthea dealbata, Cydonia oblonga, Dalbergia monetaria, Davallia divaricata, Desmodium spp., Dicksonia squarosa, Dibeteropogon amplectens, Dioclea spp, Dolichos spp., Dorycnium rectum, Echinochloa pyramidalis, Ehraffia spp., Eleusine coracana, Eragrestis spp., Erythrina spp., Eucalyptus spp., Euclea schimperi, Eulalia vi/losa, Pagopyrum spp., Feijoa sellowlana, Fragaria spp., Flemingia spp, Freycinetia banksli, Geranium thunbergii, GinAgo biloba, Glycine javanica, Gliricidia spp, Gossypium hirsutum, Grevillea spp., Guibourtia coleosperma, Hedysarum spp., Hemaffhia altissima, Heteropogon contoffus, Hordeum vulgare, Hyparrhenia rufa, Hypericum erectum, Hypeffhelia dissolute, Indigo incamata, Iris spp., Leptarrhena pyrolifolia, Lespediza spp., Lettuca spp., Leucaena leucocephala, Loudetia simplex, Lotonus bainesli, Lotus spp., Macrotyloma axillare, Malus spp., Manihot esculenta, Medicago saliva, Metasequoia glyptostroboides, Musa sapientum, Nicotianum spp., Onobrychis spp., Ornithopus spp., Oryza spp., Peltophorum africanum, Pennisetum spp., Persea gratissima, Petunia spp., Phaseolus spp., Phoenix canariensis, Phormium cookianum, Photinia spp., Picea glauca, Pinus spp., Pisum sativam, Podocarpus totara, Pogonarthria fleckii, Pogonaffhria squarrosa, Populus spp., Prosopis cineraria, Pseudotsuga menziesii, Pterolobium stellatum, Pyrus communis, Quercus spp., Rhaphiolepsis umbellata, Rhopalostylis sapida, Rhus natalensis, Ribes grossularia, Ribes spp., Robinia pseudoacacia, Rosa spp., Rubus spp., Salix spp., Schyzachyrium sanguineum, Sciadopitys vefficillata, Sequoia sempervirens, Sequoiadendron giganteum, Sorghum bicolor, Spinacia spp., Sporobolus fimbriatus, Stiburus alopecuroides, Stylosanthos humilis, Tadehagi spp, Taxodium distichum, Themeda triandra, Trifolium spp., Triticum spp., Tsuga heterophylla, Vaccinium spp., Vicia spp., Vitis vinifera, Watsonia pyramidata, Zantedeschia aethiopica, Zea mays, amaranth, artichoke, asparagus, broccoli, Brussels sprouts, cabbage, canola, carrot, cauliflower, celery, collard greens, flax, kale, lentil, oilseed rape, okra, onion, potato, rice, soybean, straw, sugar beet, sugar cane, sunflower, tomato, squash tea, maize, wheat, barely, rye, oat, peanut, pea, lentil and alfalfa, cotton, rapeseed, canola, pepper, sunflower, tobacco, eggplant, eucalyptus, a tree, an ornamental plant, a perennial grass and a forage crop. Alternatively algae and other non-Viridiplantae can be used for the methods of the present invention.
According to some embodiments of the invention, the plant used by the method of the invention is a crop plant including, but not limited to, cotton, Brassica vegetables, oilseed rape, sesame, olive tree, palm oil, banana, wheat, corn or maize, barley, alfalfa, peanuts, sunflowers, rice, oats, sugarcane, soybean, turf grasses, barley, rye, sorghum, sugar cane, chicory, lettuce, tomato, zucchini, bell pepper, eggplant, cucumber, melon, watermelon, beans, hibiscus, okra, apple, rose, strawberry, chile, garlic, pea, lentil, canola, mums, arabidopsis, broccoli, cabbage, beet, quinoa, spinach, squash, onion, leek, tobacco, potato, sugarbeet, papaya, pineapple, mango, Arabidopsis thaliana, and also plants used in horticulture, floriculture or forestry, such as, but not limited to, poplar, fir, eucalyptus, pine, an ornamental plant, a perennial grass and a forage crop, coniferous plants, moss, algae, as well as other plants listed in World Wide Web (dot) nationmaster (dot) com/encyclopedia/Plantae.
According to a specific embodiment of the present invention, the plant comprises corn.
According to a specific embodiment of the present invention, the plant comprises sorghum. 
As used herein, the phrase “exogenous polynucleotide” refers to a heterologous nucleic acid sequence or amino acid sequence which may not be naturally expressed within the plant or which overexpression in the plant is desired. The exogenous polynucleotide may be introduced into the plant in a stable or transient manner, so as to produce a ribonucleic acid (RNA) molecule or a polypeptide. It should be noted that the exogenous polynucleotide may comprise a nucleic acid sequence which is identical or partially homologous to an endogenous nucleic acid sequence of the plant.
A “transgenic plant” refers to a plant that has incorporated a nucleic acid sequence (i.e., polynucleotides encoding target genes or a silencing polynucleotide), including but not limited to genes that are not normally present in a host plant genome, nucleic acid sequences not normally transcribed into RNA, or any other genes or nucleic acid sequences that one desires to exogenously introduce into the wild-type plant, such as genes that normally may be present in the wild-type plant (control) but that one desires either to genetically engineer or to have altered expression.
Also contemplated are hybrids of the above described transgenic plants. A “hybrid plant” refers to a plant or a part thereof resulting from a cross between two parent plants, wherein one parent is a genetically engineered plant of the invention (transgenic plant expressing the polypeptides of the present invention). Such a cross can occur naturally by, for example, sexual reproduction, or artificially by, for example, in vitro nuclear fusion. Methods of plant breeding are well-known and within the level of one of ordinary skill in the art of plant biology.
The terms siRNA and miRNA are collectively referred to herein as RNA interfering molecules (RNAi).
As used herein, the phrase “siRNA” (also referred to herein interchangeably as “small interfering RNA” or “silencing RNA”, is a class of double-stranded RNA molecules, 20-25 nucleotides in length. The most notable role of siRNA is its involvement in the RNA interference (RNAi) pathway, where it interferes with the expression of a specific gene.
The siRNA precursor relates to a long dsRNA structure (at least 90% complementarity) of at least 30 bp.
As used herein, the phrase “microRNA (also referred to herein interchangeably as “miRNA” or “miR”) or a precursor thereof” refers to a microRNA (miRNA) molecule acting as a post-transcriptional regulator. Typically, the miRNA molecules are RNA molecules of about 20 to 22 nucleotides in length which can be loaded into a RISC complex and which direct the cleavage of another RNA molecule, wherein the other RNA molecule comprises a nucleotide sequence essentially complementary to the nucleotide sequence of the miRNA molecule.
Typically, a miRNA molecule is processed from a “pre-miRNA” or as used herein a precursor of a pre-miRNA molecule by proteins, such as DCL proteins, present in any plant cell and loaded onto a RISC complex where it can guide the cleavage of the target RNA molecules.
Pre-microRNA molecules are typically processed from pri-microRNA molecules (primary transcripts). The single stranded RNA segments flanking the pre-microRNA are important for processing of the pri-miRNA into the pre-miRNA. The cleavage site appears to be determined by the distance from the stem-ssRNA junction (Han et al. 2006, Cell 125, 887-901, 887-901).
As used herein, a “pre-miRNA” molecule is an RNA molecule of about 100 to about 200 nucleotides, preferably about 100 to about 130 nucleotides which can adopt a secondary structure comprising a double stranded RNA stem and a single stranded RNA loop (also referred to as “hairpin”) and further comprising the nucleotide sequence of the miRNA (and its complement sequence) in the double stranded RNA stem. According to a specific embodiment, the miRNA and its complement are located about 10 to about 20 nucleotides from the free ends of the miRNA double stranded RNA stem. The length and sequence of the single stranded loop region are not critical and may vary considerably, e.g. between 30 and 50 nt in length. The complementarity between the miRNA and its complement need not be perfect and about 1 to 3 bulges of unpaired nucleotides can be tolerated. The secondary structure adopted by an RNA molecule can be predicted by computer algorithms conventional in the art such as mFOLD. The particular strand of the double stranded RNA stem from the pre-miRNA which is released by DCL activity and loaded onto the RISC complex is determined by the degree of complementarity at the 5′ end, whereby the strand which at its 5′ end is the least involved in hydrogen bounding between the nucleotides of the different strands of the cleaved dsRNA stem is loaded onto the RISC complex and will determine the sequence specificity of the target RNA molecule degradation. However, if empirically the miRNA molecule from a particular synthetic pre-miRNA molecule is not functional (because the “wrong” strand is loaded on the RISC complex), it will be immediately evident that this problem can be solved by exchanging the position of the miRNA molecule and its complement on the respective strands of the dsRNA stem of the pre-miRNA molecule. As is known in the art, binding between A and U involving two hydrogen bounds, or G and U involving two hydrogen bounds is less strong that between G and C involving three hydrogen bounds. Exemplary siRNA sequences or precursors thereof as well as hairpin sequences and miRNA sequences are provided in Tables 1-4, below.
The tables provided in the Examples section are to be considered an integral part of the specification.
As used herein a “target gene” refers to a gene that is processed by microRNA activity. Typically the gene encodes a polypeptide which expression is downregulated due to microRNA processing.
Target genes are typically identified using the WMD3 website (http://wmd3dotweigelworlddotorg/).
As mentioned, the method of the present invention is effected by expressing within a plant an exogenous polynucleotide encoding a target gene of the RNA interfering molecules uncovered by the present inventors, as explained below.
As used herein, the phrase “expressing within the plant an exogenous polynucleotide” refers to upregulating the expression level of an exogenous polynucleotide within the plant e.g., by introducing the exogenous polynucleotide into a plant or plant cell and expressing by recombinant means, as described in detail hereinbelow.
As used herein “expressing” refers to expression at the mRNA level (e.g., in case the target gene expresses an mRNA product but no protein) or at the polypeptide level of the desired exogenous polynucleotide.
As used herein, the phrase “exogenous polynucleotide” refers to a heterologous nucleic acid sequence which may not be naturally expressed within the plant or which overexpression in the plant is desired (i.e., overexpression of an endogenous gene). The exogenous polynucleotide may be introduced into the plant in a stable or transient manner, so as to produce a ribonucleic acid (RNA) molecule and/or a polypeptide molecule. The exogenous polynucleotide may comprise a nucleic acid sequence which is identical or partially homologous to an endogenous nucleic acid sequence expressed within the plant.
The term “endogenous” as used herein refers to any polynucleotide or polypeptide which is present and/or naturally expressed within a plant or a cell thereof.
As used herein the term “polynucleotide” refers to a single or double stranded nucleic acid sequence which is isolated and provided in the form of an RNA sequence, a complementary polynucleotide sequence (cDNA), a genomic polynucleotide sequence (e.g. sequence isolated from a chromosome) and/or a composite polynucleotide sequences (e.g., a combination of the above). This term includes polynucleotides and/or oligonucleotides derived from naturally occurring nucleic acid molecules (e.g., RNA or DNA), synthetic polynucleotide and/or oligonucleotide molecules composed of naturally occurring bases, sugars, and covalent internucleoside linkages (e.g., backbone), as well as synthetic polynucleotides and/or oligonucleotides having non-naturally occurring portions, which function similarly to the respective naturally occurring portions.
The term “isolated” refers to at least partially separated from the natural environment e.g., from a plant cell.
Nucleic acid sequences of the polypeptides of some embodiments of the invention may be optimized for expression in a specific plant host. Examples of such sequence modifications include, but are not limited to, an altered G/C content to more closely approach that typically found in the plant species of interest, and the removal of codons atypically found in the plant species commonly referred to as codon optimization.
The phrase “codon optimization” refers to the selection of appropriate DNA nucleotides for use within a structural gene or fragment thereof that approaches codon usage within the plant of interest. Therefore, an optimized gene or nucleic acid sequence refers to a gene in which the nucleotide sequence of a native or naturally occurring gene has been modified in order to utilize statistically-preferred or statistically-favored codons within the plant. The nucleotide sequence typically is examined at the DNA level and the coding region optimized for expression in the plant species determined using any suitable procedure, for example as described in Sardana et al. (1996, Plant Cell Reports 15:677-681). In this method, the standard deviation of codon usage, a measure of codon usage bias, may be calculated by first finding the squared proportional deviation of usage of each codon of the native gene relative to that of highly expressed plant genes, followed by a calculation of the average squared deviation. The formula used is: 1 SDCU=n=1 N [(Xn−Yn)/Yn]2/N, where Xn refers to the frequency of usage of codon n in highly expressed plant genes, where Yn to the frequency of usage of codon n in the gene of interest and N refers to the total number of codons in the gene of interest. A table of codon usage from highly expressed genes of dicotyledonous plants is compiled using the data of Murray et al. (1989, Nuc Acids Res. 17:477-498).
One method of optimizing the nucleic acid sequence in accordance with the preferred codon usage for a particular plant cell type is based on the direct use, without performing any extra statistical calculations, of codon optimization tables such as those provided on-line at the Codon Usage Database through the NIAS (National Institute of Agrobiological Sciences) DNA bank in Japan (wwwdotkazusadotordotjp/codon/). The Codon Usage Database contains codon usage tables for a number of different species, with each codon usage table having been statistically determined based on the data present in Genbank.
By using the above tables to determine the most preferred or most favored codons for each amino acid in a particular species (for example, rice), a naturally-occurring nucleotide sequence encoding a protein of interest can be codon optimized for that particular plant species. This is effected by replacing codons that may have a low statistical incidence in the particular species genome with corresponding codons, in regard to an amino acid, that are statistically more favored. However, one or more less-favored codons may be selected to delete existing restriction sites, to create new ones at potentially useful junctions (5′ and 3′ ends to add signal peptide or termination cassettes, internal sites that might be used to cut and splice segments together to produce a correct full-length sequence), or to eliminate nucleotide sequences that may negatively effect mRNA stability or expression.
The naturally-occurring encoding nucleotide sequence may already, in advance of any modification, contain a number of codons that correspond to a statistically-favored codon in a particular plant species. Therefore, codon optimization of the native nucleotide sequence may comprise determining which codons, within the native nucleotide sequence, are not statistically-favored with regards to a particular plant, and modifying these codons in accordance with a codon usage table of the particular plant to produce a codon optimized derivative. A modified nucleotide sequence may be fully or partially optimized for plant codon usage provided that the protein encoded by the modified nucleotide sequence is produced at a level higher than the protein encoded by the corresponding naturally occurring or native gene. Construction of synthetic genes by altering the codon usage is described in for example PCT Patent Application 93/07278.
Target genes which are contemplated according to the present teachings are provided in the polynucleotide sequences which comprise nucleic acid sequences as set forth in SEQ ID NO: 1392-1646, 1655-1888, 1911-1938, 3082-3316. However the present teachings also relate to orthologs or homologs at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, or at least about 95% or more identical or similar to SEQ ID NO: 1392-1646, 1655-1888, 1911-1938, 3082-3316. Parameters for determining the level of identity are provided hereinbelow.
Alternatively or additionally, target genes which are contemplated according to the present teachings are provided in the polypeptide sequences which comprise amino acid sequences as set forth in SEQ ID NO: 687-981, 992-1248, 1281-1310, 1389-1391, and 2806-3081 (Tables 6 and 8). However the present teachings also relate to of orthologs or homologs at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, or at least about 95% or more identical or similar to SEQ ID NO: 687-981, 992-1248, 1281-1310, 1389-1391, and 2806-3081 (Tables 6 and 8).
Homology (e.g., percent homology, identity+similarity) can be determined using any homology comparison software, including for example, the TBLASTN software of the National Center of Biotechnology Information (NCBI) such as by using default parameters, when starting from a polypeptide sequence; or the tBLASTX algorithm (available via the NCBI) such as by using default parameters, which compares the six-frame conceptual translation products of a nucleotide query sequence (both strands) against a protein sequence database.
According to some embodiments of the invention, the term “homology” or “homologous” refers to identity of two or more nucleic acid sequences; or identity of two or more amino acid sequences.
Homologous sequences include both orthologous and paralogous sequences. The term “paralogous” relates to gene-duplications within the genome of a species leading to paralogous genes. The term “orthologous” relates to homologous genes in different organisms due to ancestral relationship.
One option to identify orthologues in monocot plant species is by performing a reciprocal blast search. This may be done by a first blast involving blasting the sequence-of-interest against any sequence database, such as the publicly available NCBI database which may be found at: Hypertext Transfer Protocol://World Wide Web (dot) ncbi (dot) nlm (dot) nih (dot) gov. The blast results may be filtered. The full-length sequences of either the filtered results or the non-filtered results are then blasted back (second blast) against the sequences of the organism from which the sequence-of-interest is derived. The results of the first and second blasts are then compared. An orthologue is identified when the sequence resulting in the highest score (best hit) in the first blast identifies in the second blast the query sequence (the original sequence-of-interest) as the best hit. Using the same rational a paralogue (homolog to a gene in the same organism) is found. In case of large sequence families, the ClustalW program may be used [Hypertext Transfer Protocol://World Wide Web (dot) ebi (dot) ac (dot) uk/Tools/clustalw2/index (dot) html], followed by a neighbor-joining tree (Hypertext Transfer Protocol://en (dot) wikipedia (dot) org/wiki/Neighbor-joining) which helps visualizing the clustering.
As mentioned the present inventors have also identified genes which down-regulation thereof may be done in order to improve their NUE, biomass, vigor, yield and abiotic stress tolerance.
Thus, according to an aspect of the invention there is provided a method of improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of a plant, the method comprising expressing within the plant an exogenous polynucleotide which downregulates an activity or expression of a polypeptide having an amino acid sequence at least 80%, 85%, 90%, 95%, or 100% homologous or identical to SEQ ID NOs: 311-514, 2007-2436, 1311-1320, 982-991, 1249-1280, 1321-1388 (Tables 5, 7 and 9), wherein the polypeptide is capable of regulating nitrogen use efficiency of the plant, thereby improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of the plant.
Down regulation of activity or expression is by at least 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% or even complete (100%) loss of activity or expression. Assays for measuring gene expression can be effected at the protein level (e.g., Western blot, ELISA) or at the mRNA level such as by RT-PCR.
According to a specific embodiment the amino acid sequence of the target gene is as set forth in SEQ ID NOs: 311-514, 2007-2436, 1311-1320, 982-991, 1249-1280, 1321-1388 of Tables 5, 7 and 9.
Alternatively or additionally, the amino acid sequence of the target gene is encoded by a polynucleotide sequence as set forth in SEQ ID NOs: 515-686, 2437-2805, 1939-1948, 1647-1654, 1889-1910, 1949-2004 of Tables 5, 7 and 9.
Examples of polynucleotide downregulating agents that inhibit (also referred to herein as inhibitors or nucleic acid agents) the expression of a target gene are given below.
1. Polynucleotide-Based Inhibition of Gene Expression.
It will be appreciated, that any of these methods when specifically referring to downregulating expression/activity of the target genes can be used, at least in part, to downregulate expression or activity of endogenous RNA molecules.
i. Sense Suppression/Cosuppression
In some embodiments of the invention, inhibition of the expression of target gene may be obtained by sense suppression or cosuppression. For cosuppression, an expression cassette is designed to express an RNA molecule corresponding to all or part of a messenger RNA encoding a target gene in the “sense” orientation. Over-expression of the RNA molecule can result in reduced expression of the native gene. Accordingly, multiple plant lines transformed with the cosuppression expression cassette are screened to identify those that show the greatest inhibition of target gene expression.
The polynucleotide used for cosuppression may correspond to all or part of the sequence encoding the target gene, all or part of the 5′ and/or 3′ untranslated region of a target transcript, or all or part of both the coding sequence and the untranslated regions of a transcript encoding the target gene. In some embodiments where the polynucleotide comprises all or part of the coding region for the target gene, the expression cassette is designed to eliminate the start codon of the polynucleotide so that no protein product will be transcribed.
Cosuppression may be used to inhibit the expression of plant genes to produce plants having undetectable protein levels for the proteins encoded by these genes. See, for example, Broin, et al., (2002) Plant Cell 15:1517-1532. Cosuppression may also be used to inhibit the expression of multiple proteins in the same plant. Methods for using cosuppression to inhibit the expression of endogenous genes in plants are described in Flavell, et al., (1995) Proc. Natl. Acad. Sci. USA 91:3590-3596; Jorgensen, et al., (1996) Plant Mol. Biol. 31:957-973; Johansen and Carrington, (2001) Plant Physiol. 126:930-938; Broin, et al., (2002) Plant Cell 15:1517-1532; Stoutjesdijk, et al., (2002) Plant Physiol. 129:1723-1731; Yu, et al., (2003) Phytochemistry 63:753-763; and U.S. Pat. Nos. 5,035,323, 5,283,185 and 5,952,657; each of which is herein incorporated by reference. The efficiency of cosuppression may be increased by including a poly-dt region in the expression cassette at a position 3′ to the sense sequence and 5′ of the polyadenylation signal. See, US Patent Publication Number 20020058815, herein incorporated by reference. Typically, such a nucleotide sequence has substantial sequence identity to the sequence of the transcript of the endogenous gene, optimally greater than about 65% sequence identity, more optimally greater than about 85% sequence identity, most optimally greater than about 95% sequence identity. See, U.S. Pat. Nos. 5,283,185 and 5,035,323; herein incorporated by reference.
Transcriptional gene silencing (TGS) may be accomplished through use of hpRNA constructs wherein the inverted repeat of the hairpin shares sequence identity with the promoter region of a gene to be silenced. Processing of the hpRNA into short RNAs which can interact with the homologous promoter region may trigger degradation or methylation to result in silencing. (Aufsatz, et al., (2002) PNAS 99(4):16499-16506; Mette, et al., (2000) EMBO J. 19(19):5194-5201),
ii. Antisense Suppression
In some embodiments of the invention, inhibition of the expression of the target gene may be obtained by antisense suppression. For antisense suppression, the expression cassette is designed to express an RNA molecule complementary to all or part of a messenger RNA encoding the target gene. Over-expression of the antisense RNA molecule can result in reduced expression of the native gene. Accordingly, multiple plant lines transformed with the antisense suppression expression cassette are screened to identify those that show the greatest inhibition of target gene expression.
The polynucleotide for use in antisense suppression may correspond to all or part of the complement of the sequence encoding the target gene, all or part of the complement of the 5′ and/or 3′ untranslated region of the target gene transcript, or all or part of the complement of both the coding sequence and the untranslated regions of a transcript encoding the target gene. In addition, the antisense polynucleotide may be fully complementary (i.e., 100% identical to the complement of the target sequence) or partially complementary (i.e., less than 100% identical to the complement of the target sequence) to the target sequence. Antisense suppression may be used to inhibit the expression of multiple proteins in the same plant. Furthermore, portions of the antisense nucleotides may be used to disrupt the expression of the target gene. Generally, sequences of at least 50 nucleotides, 100 nucleotides, 200 nucleotides, 300, 500, 550, 500, 550 or greater may be used. Methods for using antisense suppression to inhibit the expression of endogenous genes in plants are described, for example, in Liu, et al., (2002) Plant Physiol. 129:1732-1753 and U.S. Pat. No. 5,759,829, which is herein incorporated by reference. Efficiency of antisense suppression may be increased by including a poly-dt region in the expression cassette at a position 3′ to the antisense sequence and 5′ of the polyadenylation signal. See, US Patent Publication Number 20020058815.
iii. Double-Stranded RNA Interference
In some embodiments of the invention, inhibition of the expression of a target gene may be obtained by double-stranded RNA (dsRNA) interference. For dsRNA interference, a sense RNA molecule like that described above for cosuppression and an antisense RNA molecule that is fully or partially complementary to the sense RNA molecule are expressed in the same cell, resulting in inhibition of the expression of the corresponding endogenous messenger RNA.
Expression of the sense and antisense molecules can be accomplished by designing the expression cassette to comprise both a sense sequence and an antisense sequence. Alternatively, separate expression cassettes may be used for the sense and antisense sequences. Multiple plant lines transformed with the dsRNA interference expression cassette or expression cassettes are then screened to identify plant lines that show the greatest inhibition of target gene expression. Methods for using dsRNA interference to inhibit the expression of endogenous plant genes are described in Waterhouse, et al., (1998) Proc. Natl. Acad. Sci. USA 95:13959-13965, Liu, et al., (2002) Plant Physiol. 129:1732-1753, and WO 99/59029, WO 99/53050, WO 99/61631, and WO 00/59035,
iv. Hairpin RNA Interference and Intron-Containing Hairpin RNA Interference
In some embodiments of the invention, inhibition of the expression of one or more target gene may be obtained by hairpin RNA (hpRNA) interference or intron-containing hairpin RNA (ihpRNA) interference. These methods are highly efficient at downregulating the expression of endogenous genes. See, Waterhouse and Helliwell, (2003) Nat. Rev. Genet. 5:29-38 and the references cited therein.
For hpRNA interference, the expression cassette is designed to express an RNA molecule that hybridizes with itself to form a hairpin structure that comprises a single-stranded loop region and a base-paired stem. The base-paired stem region comprises a sense sequence corresponding to all or part of the endogenous messenger RNA encoding the gene whose expression is to be inhibited, and an antisense sequence that is fully or partially complementary to the sense sequence. Thus, the base-paired stem region of the molecule generally determines the specificity of the RNA interference. hpRNA molecules are highly efficient at inhibiting the expression of endogenous genes, and the RNA interference they induce is inherited by subsequent generations of plants. See, for example, Chuang and Meyerowitz, (2000) Proc. Natl. Acad. Sci. USA 97:5985-5990; Stoutjesdijk, et al., (2002) Plant Physiol. 129:1723-1731; and Waterhouse and Helliwell, (2003) Nat. Rev. Genet. 5:29-38. Methods for using hpRNA interference to inhibit or silence the expression of genes are described, for example, in Chuang and Meyerowitz, (2000) Proc. Natl. Acad. Sci. USA 97:5985-5990; Stoutjesdijk, et al., (2002) Plant Physiol. 129:1723-1731; Waterhouse and Helliwell, (2003) Nat. Rev. Genet. 5:29-38; Pandolfini, et al., BMC Biotechnology 3:7, and US Patent Publication Number 20030175965; each of which is herein incorporated by reference. A transient assay for the efficiency of hpRNA constructs to silence gene expression in vivo has been described by Panstruga, et al., (2003) Mol. Biol. Rep. 30:135-150, herein incorporated by reference.
ForihpRNA, the interfering molecules have the same general structure as for hpRNA, but the RNA molecule additionally comprises an intron that is capable of being spliced in the cell in which the ihpRNA is expressed. The use of an intron minimizes the size of the loop in the hairpin RNA molecule following splicing, and this increases the efficiency of interference. See, for example, Smith, et al., (2000) Nature 507:319-320. In fact, Smith, et al., show 100% suppression of endogenous gene expression using ihpRNA-mediated interference. Methods for using ihpRNA interference to inhibit the expression of endogenous plant genes are described, for example, in Smith, et al., (2000) Nature 507:319-320; Wesley, et al., (2001) Plant J. 27:581-590; Wang and Waterhouse, (2001) Curr. Opin. Plant Biol. 5:156-150; Waterhouse and Helliwell, (2003) Nat. Rev. Genet. 5:29-38; Helliwell and Waterhouse, (2003) Methods 30:289-295, and US Patent Publication Number 20030180955, each of which is herein incorporated by reference.
The expression cassette for hpRNA interference may also be designed such that the sense sequence and the antisense sequence do not correspond to an endogenous RNA. In this embodiment, the sense and antisense sequence flank a loop sequence that comprises a nucleotide sequence corresponding to all or part of the endogenous messenger RNA of the target gene. Thus, it is the loop region that determines the specificity of the RNA interference. See, for example, WO 02/00905, herein incorporated by reference.
v. Amplicon-Mediated Interference
Amplicon expression cassettes comprise a plant virus-derived sequence that contains all or part of the target gene but generally not all of the genes of the native virus. The viral sequences present in the transcription product of the expression cassette allow the transcription product to direct its own replication. The transcripts produced by the amplicon may be either sense or antisense relative to the target sequence (i.e., the messenger RNA for target gene). Methods of using amplicons to inhibit the expression of endogenous plant genes are described, for example, in Angell and Baulcombe, (1997) EMBO J. 16:3675-3685, Angell and Baulcombe, (1999) Plant J. 20:357-362, and U.S. Pat. No. 6,656,805, each of which is herein incorporated by reference.
vi. Ribozymes
In some embodiments, the polynucleotide expressed by the expression cassette of the invention is catalytic RNA or has ribozyme activity specific for the messenger RNA of target gene. Thus, the polynucleotide causes the degradation of the endogenous messenger RNA, resulting in reduced expression of the target gene. This method is described, for example, in U.S. Pat. No. 5,987,071, herein incorporated by reference.
2. Gene Disruption
In some embodiments of the present invention, the activity of a miRNA or a target gene is reduced or eliminated by disrupting the gene encoding the target polypeptide. The gene encoding the target polypeptide may be disrupted by any method known in the art. For example, in one embodiment, the gene is disrupted by transposon tagging. In another embodiment, the gene is disrupted by mutagenizing plants using random or targeted mutagenesis, and selecting for plants that have reduced response regulator activity.
Any of the nucleic acid agents described herein (for overexpression or downregulation) can be provided to the plant as naked RNA or expressed from a nucleic acid expression construct, where it is operaly linked to a regulatory sequence.
According to a specific embodiment of the invention, there is provided a nucleic acid construct comprising a nucleic acid sequence (a polynucleotide) encoding the RNAi target polypeptide or the nucleic acid agent for downregulating the expression of the target gene, the nucleic acid sequence being under a transcriptional control a cis-acting regulatory element.
Exemplary nucleic acid constructs which can be used for plant transformation include, but are not limited to, pORE156, pORE164, pORE167 and pORE169, which are all constructed by ligating the appropriate DNA fragments into the pORE E2 binary vector (Accession number: AY562535, FIG. 1) under the transcriptional control of a promoter.
A coding nucleic acid sequence is “operably linked” or “transcriptionally linked to a regulatory sequence (e.g., promoter)” if the regulatory sequence is capable of exerting a regulatory effect on the coding sequence linked thereto. Thus the regulatory sequence controls the transcription of the target polynucleotide.
The term “regulatory sequence”, as used herein, means any DNA, that is involved in driving transcription and controlling (i.e., regulating) the timing and level of transcription of a given DNA sequence, such as a DNA coding for the target polypeptide, as described above. For example, a 5′ regulatory region (or “promoter region”) is a DNA sequence located upstream (i.e., 5′) of a coding sequence and which comprises the promoter and the 5′-untranslated leader sequence. A 3′ regulatory region is a DNA sequence located downstream (i.e., 3′) of the coding sequence and which comprises suitable transcription termination (and/or regulation) signals, including one or more polyadenylation signals.
For the purpose of the invention, the promoter is a plant-expressible promoter. As used herein, the term “plant-expressible promoter” means a DNA sequence which is capable of controlling (initiating) transcription in a plant cell. This includes any promoter of plant origin, but also any promoter of non-plant origin which is capable of directing transcription in a plant cell, i.e., certain promoters of viral or bacterial origin Thus, any suitable promoter sequence can be used by the nucleic acid construct of the present invention. According to some embodiments of the invention, the promoter is a constitutive promoter, a tissue-specific promoter or an inducible promoter (e.g. an abiotic stress-inducible promoter).
Suitable constitutive promoters include, for example, hydroperoxide lyase (HPL) promoter, CaMV 35S promoter (Odell et al, Nature 313:810-812, 1985); Arabidopsis At6669 promoter (see PCT Publication No. WO04081173A2); Arabidopsis new At6669 promoter; maize Ubi 1 (Christensen et al., Plant Sol. Biol. 18:675-689, 1992); rice actin (McElroy et al., Plant Cell 2:163-171, 1990); pEMU (Last et al, Theor. Appl. Genet. 81:581-588, 1991); CaMV 19S (Nilsson et al, Physiol. Plant 100:456-462, 1997); GOS2 (de Pater et al, Plant J November; 2(6):837-44, 1992); ubiquitin (Christensen et al, Plant MoI. Biol. 18: 675-689, 1992); Rice cyclophilin (Bucholz et al, Plant MoI Biol. 25(5):837-43, 1994); Maize H3 histone (Lepetit et al, MoI. Gen. Genet. 231: 276-285, 1992); Actin 2 (An et al, Plant J. 10(1);107-121, 1996) and Synthetic Super MAS (Ni et al., The Plant Journal 7: 661-76, 1995). Other constitutive promoters include those in U.S. Pat. Nos. 5,659,026, 5,608,149; 5,608,144; 5,604,121; 5,569,597; 5,466,785; 5,399,680; 5,268,463; and 5,608,142.
Suitable tissue-specific promoters include, but not limited to, leaf-specific promoters [such as described, for example, by Yamamoto et al., Plant J. 12:255-265, 1997; Kwon et al., Plant Physiol. 105:357-67, 1994; Yamamoto et al., Plant Cell Physiol. 35:773-778, 1994; Gotor et al., Plant J. 3:509-18, 1993; Orozco et al., Plant MoI. Biol. 23:1129-1138, 1993; and Matsuoka et al., Proc. Natl. Acad. Sci. USA 90:9586-9590, 1993], seed-preferred promoters [e.g., from seed specific genes (Simon, et al., Plant MoI. Biol. 5. 191, 1985; Scofield, et al., J. Biol. Chem. 262: 12202, 1987; Baszczynski, et al., Plant MoI. Biol. 14: 633, 1990), Brazil Nut albumin (Pearson′ et al., Plant MoI. Biol. 18: 235-245, 1992), legumin (Ellis, et al. Plant MoI. Biol. 10: 203-214, 1988), Glutelin (rice) (Takaiwa, et al., MoI. Gen. Genet. 208: 15-22, 1986; Takaiwa, et al., FEBS Letts. 221: 43-47, 1987), Zein (Matzke et al., Plant MoI Biol, 143)323-32 1990), napA (Stalberg, et al., Planta 199: 515-519, 1996), Wheat SPA (Albani et al, Plant Cell, 9: 171-184, 1997), sunflower oleosin (Cummins, et al, Plant MoI. Biol. 19: 873-876, 1992)], endosperm specific promoters [e.g., wheat LMW and HMW, glutenin-1 (MoI Gen Genet 216:81-90, 1989; NAR 17:461-2), wheat a, b and g gliadins (EMBO3: 1409-15, 1984), Barley ltrl promoter, barley Bl, C, D hordein (Theor Appl Gen 98:1253-62, 1999; Plant J 4:343-55, 1993; MoI Gen Genet 250:750-60, 1996), Barley DOF (Mena et al., The Plant Journal, 116(1): 53-62, 1998), Biz2 (EP99106056.7), Synthetic promoter (Vicente-Carbajosa et al., Plant J. 13: 629-640, 1998), rice prolamin NRP33, rice-globulin GIb-I (Wu et al., Plant Cell Physiology 39(8) 885-889, 1998), rice alpha-globulin REB/OHP-1 (Nakase et al. Plant MoI. Biol. 33: 513-S22, 1997), rice ADP-glucose PP (Trans Res 6:157-68, 1997), maize ESR gene family (Plant J 12:235-46, 1997), sorghum gamma-kafirin (PMB 32:1029-35, 1996); e.g., the Napin promoter], embryo specific promoters [e.g., rice OSH1 (Sato et al, Proc. Natl. Acad. Sci. USA, 93: 8117-8122), KNOX (Postma-Haarsma et al, Plant MoI. Biol. 39:257-71, 1999), rice oleosin (Wu et at, J. Biochem., 123:386, 1998)], and flower-specific promoters [e.g., AtPRP4, chalene synthase (chsA) (Van der Meer, et al., Plant MoI. Biol. 15, 95-109, 1990), LAT52 (Twell et al., MoI. Gen Genet. 217:240-245; 1989), apetala-3]. Also contemplated are root-specific promoters such as the ROOTP promoter described in Vissenberg K, et al. Plant Cell Physiol. 2005 January; 46(1):192-200.
The nucleic acid construct of some embodiments of the invention can further include an appropriate selectable marker and/or an origin of replication.
The nucleic acid construct of some embodiments of the invention can be utilized to stably or transiently transform plant cells. In stable transformation, the exogenous polynucleotide is integrated into the plant genome and as such it represents a stable and inherited trait. In transient transformation, the exogenous polynucleotide is expressed by the cell transformed but it is not integrated into the genome and as such it represents a transient trait.
There are various methods of introducing foreign genes into both monocotyledonous and dicotyledonous plants (Potrykus, L, Annu. Rev. Plant. Physiol, Plant. MoI. Biol. (1991) 42:205-225; Shimamoto et al., Nature (1989) 338:274-276).
The principle methods of causing stable integration of exogenous DNA into plant genomic DNA include two main approaches:
(i) Agrobacterium-mediated gene transfer (e.g., T-DNA using Agrobacterium tumefaciens or Agrobacterium rhizogenes); see for example, Klee et al. (1987) Annu. Rev. Plant Physiol. 38:467-486; Klee and Rogers in Cell Culture and Somatic Cell Genetics of Plants, Vol. 6, Molecular Biology of Plant Nuclear Genes, eds. Schell, J., and Vasil, L. K., Academic Publishers, San Diego, Calif. (1989) p. 2-25; Gatenby, in Plant Biotechnology, eds. Kung, S, and Arntzen, C. J., Butterworth Publishers, Boston, Mass. (1989) p. 93-112.
(ii) Direct DNA uptake: Paszkowski et al., in Cell Culture and Somatic Cell Genetics of Plants, Vol. 6, Molecular Biology of Plant Nuclear Genes eds. Schell, J., and Vasil, L. K., Academic Publishers, San Diego, Calif. (1989) p. 52-68; including methods for direct uptake of DNA into protoplasts, Toriyama, K. et al. (1988) Bio/Technology 6:1072-1074. DNA uptake induced by brief electric shock of plant cells: Zhang et al. Plant Cell Rep. (1988) 7:379-384. Fromm et al. Nature (1986) 319:791-793. DNA injection into plant cells or tissues by particle bombardment, Klein et al. Bio/Technology (1988) 6:559-563; McCabe et al. Bio/Technology (1988) 6:923-926; Sanford, Physiol. Plant. (1990) 79:206-209; by the use of micropipette systems: Neuhaus et al., Theor. Appl. Genet. (1987) 75:30-36; Neuhaus and Spangenberg, Physiol. Plant. (1990) 79:213-217; glass fibers or silicon carbide whisker transformation of cell cultures, embryos or callus tissue, U.S. Pat. No. 5,464,765 or by the direct incubation of DNA with germinating pollen, DeWet et al. in Experimental Manipulation of Ovule Tissue, eds. Chapman, G. P. and Mantell, S. H. and Daniels, W. Longman, London, (1985) p. 197-209; and Ohta, Proc. Natl. Acad. Sci. USA (1986) 83:715-719.
The Agrobacterium system includes the use of plasmid vectors that contain defined DNA segments that integrate into the plant genomic DNA. Methods of inoculation of the plant tissue vary depending upon the plant species and the Agrobacterium delivery system. A widely used approach is the leaf disc procedure which can be performed with any tissue explant that provides a good source for initiation of whole plant differentiation. See, e.g., Horsch et al. in Plant Molecular Biology Manual A5, Kluwer Academic Publishers, Dordrecht (1988) p. 1-9. A supplementary approach employs the Agrobacterium delivery system in combination with vacuum infiltration. The Agrobacterium system is especially viable in the creation of transgenic dicotyledonous plants.
According to a specific embodiment of the present invention, the exogenous polynucleotide is introduced into the plant by infecting the plant with a bacteria, such as using a floral dip transformation method (as described in further detail in Example 5, of the Examples section which follows).
There are various methods of direct DNA transfer into plant cells. In electroporation, the protoplasts are briefly exposed to a strong electric field. In microinjection, the DNA is mechanically injected directly into the cells using very small micropipettes. In microparticle bombardment, the DNA is adsorbed on microprojectiles such as magnesium sulfate crystals or tungsten particles, and the microprojectiles are physically accelerated into cells or plant tissues.
Following stable transformation plant propagation is exercised. The most common method of plant propagation is by seed. Regeneration by seed propagation, however, has the deficiency that due to heterozygosity there is a lack of uniformity in the crop, since seeds are produced by plants according to the genetic variances governed by Mendelian rules. Basically, each seed is genetically different and each will grow with its own specific traits. Therefore, it is preferred that the transformed plant be produced such that the regenerated plant has the identical traits and characteristics of the parent transgenic plant. For this reason it is preferred that the transformed plant be regenerated by micropropagation which provides a rapid, consistent reproduction of the transformed plants.
Micropropagation is a process of growing new generation plants from a single piece of tissue that has been excised from a selected parent plant or cultivar. This process permits the mass reproduction of plants having the preferred tissue expressing the fusion protein. The new generation plants which are produced are genetically identical to, and have all of the characteristics of, the original plant. Micropropagation allows mass production of quality plant material in a short period of time and offers a rapid multiplication of selected cultivars in the preservation of the characteristics of the original transgenic or transformed plant. The advantages of cloning plants are the speed of plant multiplication and the quality and uniformity of plants produced.
Micropropagation is a multi-stage procedure that requires alteration of culture medium or growth conditions between stages. Thus, the micropropagation process involves four basic stages: Stage one, initial tissue culturing; stage two, tissue culture multiplication; stage three, differentiation and plant formation; and stage four, greenhouse culturing and hardening. During stage one, initial tissue culturing, the tissue culture is established and certified contaminant-free. During stage two, the initial tissue culture is multiplied until a sufficient number of tissue samples are produced to meet production goals. During stage three, the tissue samples grown in stage two are divided and grown into individual plantlets. At stage four, the transformed plantlets are transferred to a greenhouse for hardening where the plants' tolerance to light is gradually increased so that it can be grown in the natural environment.
Although stable transformation is presently preferred, transient transformation of leaf cells, meristematic cells or the whole plant is also envisaged by the present invention.
Transient transformation can be effected by any of the direct DNA transfer methods described above or by viral infection using modified plant viruses.
Viruses that have been shown to be useful for the transformation of plant hosts include CaMV, Tobacco mosaic virus (TMV), brome mosaic virus (BMV) and Bean Common Mosaic Virus (BV or BCMV). Transformation of plants using plant viruses is described in U.S. Pat. No. 4,855,237 (bean golden mosaic virus; BGV), EP-A 67,553 (TMV), Japanese Published Application No. 63-14693 (TMV), EPA 194,809 (BV), EPA 278,667 (BV); and Gluzman, Y. et al., Communications in Molecular Biology: Viral Vectors, Cold Spring Harbor Laboratory, New York, pp. 172-189 (1988). Pseudovirus particles for use in expressing foreign DNA in many hosts, including plants are described in WO 87/06261. According to some embodiments of the invention, the virus used for transient transformations is avirulent and thus is incapable of causing severe symptoms such as reduced growth rate, mosaic, ring spots, leaf roll, yellowing, streaking, pox formation, tumor formation and pitting. A suitable avirulent virus may be a naturally occurring avirulent virus or an artificially attenuated virus. Virus attenuation may be effected by using methods well known in the art including, but not limited to, sub-lethal heating, chemical treatment or by directed mutagenesis techniques such as described, for example, by Kurihara and Watanabe (Molecular Plant Pathology 4:259-269, 2003), Galon et al. (1992), Atreya et al. (1992) and Huet et al. (1994).
Suitable virus strains can be obtained from available sources such as, for example, the American Type culture Collection (ATCC) or by isolation from infected plants. Isolation of viruses from infected plant tissues can be effected by techniques well known in the art such as described, for example by Foster and Tatlor, Eds. “Plant Virology Protocols: From Virus Isolation to Transgenic Resistance (Methods in Molecular Biology (Humana Pr), VoI 81)”, Humana Press, 1998. Briefly, tissues of an infected plant believed to contain a high concentration of a suitable virus, preferably young leaves and flower petals, are ground in a buffer solution (e.g., phosphate buffer solution) to produce a virus infected sap which can be used in subsequent inoculations.
Construction of plant RNA viruses for the introduction and expression of non-viral exogenous polynucleotide sequences in plants is demonstrated by the above references as well as by Dawson, W. O. et al, Virology (1989) 172:285-292; Takamatsu et al. EMBO J. (1987) 6:307-311; French et al. Science (1986) 231:1294-1297; Takamatsu et al. FEBS Letters (1990) 269:73-76; and U.S. Pat. No. 5,316,931.
When the virus is a DNA virus, suitable modifications can be made to the virus itself. Alternatively, the virus can first be cloned into a bacterial plasmid for ease of constructing the desired viral vector with the foreign DNA. The virus can then be excised from the plasmid. If the virus is a DNA virus, a bacterial origin of replication can be attached to the viral DNA, which is then replicated by the bacteria. Transcription and translation of this DNA will produce the coat proteins which will encapsidate the viral DNA. If the virus is an RNA virus, the virus is generally cloned as a cDNA and inserted into a plasmid. The plasmid is then used to make all of the constructions. The RNA virus is then produced by transcribing the viral sequence of the plasmid and translation of the viral genes to produce the coat protein(s) which encapsidate the viral RNA.
In one embodiment, a plant viral nucleic acid is provided in which the native coat protein coding sequence has been deleted from a viral nucleic acid, a non-native plant viral coat protein coding sequence and a non-native promoter, preferably the subgenomic promoter of the non-native coat protein coding sequence, capable of expression in the plant host, packaging of the recombinant plant viral nucleic acid, and ensuring a systemic infection of the host by the recombinant plant viral nucleic acid, has been inserted. Alternatively, the coat protein gene may be inactivated by insertion of the non-native nucleic acid sequence within it, such that a protein is produced. The recombinant plant viral nucleic acid may contain one or more additional non-native subgenomic promoters. Each non-native subgenomic promoter is capable of transcribing or expressing adjacent genes or nucleic acid sequences in the plant host and incapable of recombination with each other and with native subgenomic promoters. Non-native (foreign) nucleic acid sequences may be inserted adjacent the native plant viral subgenomic promoter or the native and a non-native plant viral subgenomic promoters if more than one nucleic acid sequence is included. The non-native nucleic acid sequences are transcribed or expressed in the host plant under control of the subgenomic promoter to produce the desired products.
In a second embodiment, a recombinant plant viral nucleic acid is provided as in the first embodiment except that the native coat protein coding sequence is placed adjacent one of the non-native coat protein subgenomic promoters instead of a non-native coat protein coding sequence.
In a third embodiment, a recombinant plant viral nucleic acid is provided in which the native coat protein gene is adjacent its subgenomic promoter and one or more non-native subgenomic promoters have been inserted into the viral nucleic acid. The inserted non-native subgenomic promoters are capable of transcribing or expressing adjacent genes in a plant host and are incapable of recombination with each other and with native subgenomic promoters. Non-native nucleic acid sequences may be inserted adjacent the non-native subgenomic plant viral promoters such that the sequences are transcribed or expressed in the host plant under control of the subgenomic promoters to produce the desired product.
In a fourth embodiment, a recombinant plant viral nucleic acid is provided as in the third embodiment except that the native coat protein coding sequence is replaced by a non-native coat protein coding sequence.
The viral vectors are encapsidated by the coat proteins encoded by the recombinant plant viral nucleic acid to produce a recombinant plant virus. The recombinant plant viral nucleic acid or recombinant plant virus is used to infect appropriate host plants. The recombinant plant viral nucleic acid is capable of replication in the host, systemic spread in the host, and transcription or expression of foreign gene(s) (isolated nucleic acid) in the host to produce the desired protein.
In addition to the above, the nucleic acid molecule of the present invention can also be introduced into a chloroplast genome thereby enabling chloroplast expression.
A technique for introducing exogenous nucleic acid sequences to the genome of the chloroplasts is known. This technique involves the following procedures. First, plant cells are chemically treated so as to reduce the number of chloroplasts per cell to about one. Then, the exogenous nucleic acid is introduced via particle bombardment into the cells with the aim of introducing at least one exogenous nucleic acid molecule into the chloroplasts. The exogenous nucleic acid is selected such that it is integratable into the chloroplast's genome via homologous recombination which is readily effected by enzymes inherent to the chloroplast. To this end, the exogenous nucleic acid includes, in addition to a gene of interest, at least one nucleic acid stretch which is derived from the chloroplast's genome. In addition, the exogenous nucleic acid includes a selectable marker, which serves by sequential selection procedures to ascertain that all or substantially all of the copies of the chloroplast genomes following such selection will include the exogenous nucleic acid. Further details relating to this technique are found in U.S. Pat. Nos. 4,945,050; and 5,693,507 which are incorporated herein by reference. A polypeptide can thus be produced by the protein expression system of the chloroplast and become integrated into the chloroplast's inner membrane.
Since NUE, abiotic stress tolerance as well as yield, vigor or biomass of the plant can involve multiple genes acting additively or in synergy (see, for example, in Quesda et al., Plant Physiol. 130:951-063, 2002), the invention also envisages expressing a plurality of exogenous polynucleotides in a single host plant to thereby achieve superior effect on NUE, abiotic stress tolerance, yield, vigor and biomass of the plant.
Expressing a plurality of exogenous polynucleotides in a single host plant can be effected by co-introducing multiple nucleic acid constructs, each including a different exogenous polynucleotide, into a single plant cell. The transformed cell can then be regenerated into a mature plant using the methods described hereinabove. Alternatively, expressing a plurality of exogenous polynucleotides in a single host plant can be effected by co-introducing into a single plant-cell a single nucleic-acid construct including a plurality of different exogenous polynucleotides. Such a construct can be designed with a single promoter sequence which can transcribe a polycistronic messenger RNA including all the different exogenous polynucleotide sequences. To enable co-translation of the different polypeptides encoded by the polycistronic messenger RNA, the polynucleotide sequences can be inter-linked via an internal ribosome entry site (IRES) sequence which facilitates translation of polynucleotide sequences positioned downstream of the IRES sequence. In this case, a transcribed polycistronic RNA molecule encoding the different polypeptides described above will be translated from both the capped 5′ end and the two internal IRES sequences of the polycistronic RNA molecule to thereby produce in the cell all different polypeptides. Alternatively, the construct can include several promoter sequences each linked to a different exogenous polynucleotide sequence.
The plant cell transformed with the construct including a plurality of different exogenous polynucleotides can be regenerated into a mature plant, using the methods described hereinabove.
Alternatively, expressing a plurality of exogenous polynucleotides can be effected by introducing different nucleic acid constructs, including different exogenous polynucleotides, into a plurality of plants. The regenerated transformed plants can then be cross-bred and resultant progeny selected for superior NUE, abiotic stress tolerance, yield as described above, using conventional plant breeding techniques.
As mentioned, expression (or reduction in a level of expression) of the target polynucleotides/polypeptides of the present invention can be qualified using methods which are well known in the art such as those involving gene amplification Western blotting, ELISA, or at the mRNA level involving e.g., PCR or RT-PCR or Northern blot or in-situ hybridization (in which one monitors the level of target gene expression).
According to some embodiments of the invention, the plant expressing the exogenous polynucleotide(s) is grown under stress (nitrogen or abiotic) or normal conditions (e.g., biotic conditions and/or conditions with sufficient water, nutrients such as nitrogen and fertilizer). Such conditions, which depend on the plant being grown, are known to those skilled in the art of agriculture, and are further, described above.
According to some embodiments of the invention, the method further comprises growing the plant expressing the exogenous polynucleotide(s) under abiotic stress or nitrogen limiting conditions. Non-limiting examples of abiotic stress conditions include, water deprivation, drought, excess of water (e.g., flood, waterlogging), freezing, low temperature, high temperature, strong winds, heavy metal toxicity, anaerobiosis, nutrient deficiency, nutrient excess, salinity, atmospheric pollution, intense light, insufficient light, or UV irradiation, etiolation and atmospheric pollution.
Thus, the invention encompasses plants exogenously expressing the polynucleotide(s), the nucleic acid constructs of the invention.
The sequence information and annotations uncovered by the present teachings can be harnessed in favor of classical breeding. Thus, sub-sequence data of those polynucleotides described above, can be used as markers for marker assisted selection (MAS), in which a marker is used for indirect selection of a genetic determinant or determinants of a trait of interest (e.g., tolerance to abiotic stress). Nucleic acid data of the present teachings (DNA or RNA sequence) may contain or be linked to polymorphic sites or genetic markers on the genome such as restriction fragment length polymorphism (RFLP), microsatellites and single nucleotide polymorphism (SNP), DNA fingerprinting (DFP), amplified fragment length polymorphism (AFLP), expression level polymorphism, and any other polymorphism at the DNA or RNA sequence.
Examples of marker assisted selections include, but are not limited to, selection for a morphological trait (e.g., a gene that affects form, coloration, male sterility or resistance such as the presence or absence of awn, leaf sheath coloration, height, grain color, aroma of rice); selection for a biochemical trait (e.g., a gene that encodes a protein that can be extracted and observed; for example, isozymes and storage proteins); selection for a biological trait (e.g., pathogen races or insect biotypes based on host pathogen or host parasite interaction can be used as a marker since the genetic constitution of an organism can affect its susceptibility to pathogens or parasites).
The polynucleotides described hereinabove can be used in a wide range of economical plants, in a safe and cost effective manner.
Plant lines exogenously expressing the polynucleotide of the invention can be screened to identify those that show the greatest increase of the desired plant trait.
Thus, according to an additional embodiment of the present invention, there is provided a method of evaluating a trait of a plant, the method comprising: (a) expressing in a plant or a portion thereof the nucleic acid construct; and (b) evaluating a trait of a plant as compared to a wild type plant of the same type; thereby evaluating the trait of the plant.
Thus, the effect of the transgene (the exogenous polynucleotide) on different plant characteristics may be determined any method known to one of ordinary skill in the art.
Thus, for example, tolerance to limiting nitrogen conditions may be compared in transformed plants {i.e., expressing the transgene) compared to non-transformed (wild type) plants exposed to the same stress conditions (other stress conditions are contemplated as well, e.g. water deprivation, salt stress e.g. salinity, suboptimal temperature, osmotic stress, and the like), using the following assays.
Methods of qualifying plants as being tolerant or having improved tolerance to abiotic stress or limiting nitrogen levels are well known in the art and are further described hereinbelow.
Fertilizer use efficiency—To analyze whether the transgenic plants are more responsive to fertilizers, plants are grown in agar plates or pots with a limited amount of fertilizer, as described, for example, in Yanagisawa et al (Proc Natl Acad Sci USA. 2004; 101:7833-8). The plants are analyzed for their overall size, time to flowering, yield, protein content of shoot and/or grain. The parameters checked are the overall size of the mature plant, its wet and dry weight, the weight of the seeds yielded, the average seed size and the number of seeds produced per plant. Other parameters that may be tested are: the chlorophyll content of leaves (as nitrogen plant status and the degree of leaf verdure is highly correlated), amino acid and the total protein content of the seeds or other plant parts such as leaves or shoots, oil content, etc. Similarly, instead of providing nitrogen at limiting amounts, phosphate or potassium can be added at increasing concentrations. Again, the same parameters measured are the same as listed above. In this way, nitrogen use efficiency (NUE), phosphate use efficiency (PUE) and potassium use efficiency (KUE) are assessed, checking the ability of the transgenic plants to thrive under nutrient restraining conditions.
Nitrogen use efficiency—To analyze whether the transgenic plants (e.g., Arabidopsis plants) are more responsive to nitrogen, plant are grown in 0.75-3 millimolar (mM, nitrogen deficient conditions) or 6-10 mM (optimal nitrogen concentration). Plants are allowed to grow for additional 25 days or until seed production. The plants are then analyzed for their overall size, time to flowering, yield, protein content of shoot and/or grain/seed production. The parameters checked can be the overall size of the plant, wet and dry weight, the weight of the seeds yielded, the average seed size and the number of seeds produced per plant. Other parameters that may be tested are: the chlorophyll content of leaves (as nitrogen plant status and the degree of leaf greenness is highly correlated), amino acid and the total protein content of the seeds or other plant parts such as leaves or shoots and oil content. Transformed plants not exhibiting substantial physiological and/or morphological effects, or exhibiting higher measured parameters levels than wild-type plants, are identified as nitrogen use efficient plants.
Nitrogen Use efficiency assay using plantlets—The assay is done according to Yanagisawa-S. et al. with minor modifications (“Metabolic engineering with Dofl transcription factor in plants: Improved nitrogen assimilation and growth under low-nitrogen conditions” Proc. Natl. Acad. Sci. USA 101, 7833-7838). Briefly, transgenic plants which are grown for 7-10 days in 0.5×MS [Murashige-Skoog] supplemented with a selection agent are transferred to two nitrogen-limiting conditions: MS media in which the combined nitrogen concentration (NH4NO3 and KNO3) was 0.75 mM (nitrogen deficient conditions) or 6-15 mM (optimal nitrogen concentration). Plants are allowed to grow for additional 30-40 days and then photographed, individually removed from the Agar (the shoot without the roots) and immediately weighed (fresh weight) for later statistical analysis. Constructs for which only T1 seeds are available are sown on selective media and at least 20 seedlings (each one representing an independent transformation event) are carefully transferred to the nitrogen-limiting media. For constructs for which T2 seeds are available, different transformation events are analyzed. Usually, 20 randomly selected plants from each event are transferred to the nitrogen-limiting media allowed to grow for 3-4 additional weeks and individually weighed at the end of that period. Transgenic plants are compared to control plants grown in parallel under the same conditions. Mock-transgenic plants expressing the uidA reporter gene (GUS) under the same promoter or transgenic plants carrying the same promoter but lacking a reporter gene are used as control.
Nitrogen determination—The procedure for N (nitrogen) concentration determination in the structural parts of the plants involves the potassium persulfate digestion method to convert organic N to NO3− (Purcell and King 1996 Argon. J. 88:111-113, the modified Cd− mediated reduction of NO3− to NO2− (Vodovotz 1996 Biotechniques 20:390-394) and the measurement of nitrite by the Griess assay (Vodovotz 1996, supra). The absorbance values are measured at 550 nm against a standard curve of NaNO2. The procedure is described in details in Samonte et al. 2006 Agron. J. 98:168-176.
Tolerance to abiotic stress (e.g. tolerance to drought or salinity) can be evaluated by determining the differences in physiological and/or physical condition, including but not limited to, vigor, growth, size, or root length, or specifically, leaf color or leaf area size of the transgenic plant compared to a non-modified plant of the same species grown under the same conditions. Other techniques for evaluating tolerance to abiotic stress include, but are not limited to, measuring chlorophyll fluorescence, photosynthetic rates and gas exchange rates. Further assays for evaluating tolerance to abiotic stress are provided hereinbelow and in the Examples section which follows.
Drought tolerance assay—Soil-based drought screens are performed with plants overexpressing the polynucleotides detailed above. Seeds from control Arabidopsis plants, or other transgenic plants overexpressing nucleic acid of the invention are germinated and transferred to pots. Drought stress is obtained after irrigation is ceased. Transgenic and control plants are compared to each other when the majority of the control plants develop severe wilting. Plants are re-watered after obtaining a significant fraction of the control plants displaying a severe wilting. Plants are ranked comparing to controls for each of two criteria: tolerance to the drought conditions and recovery (survival) following re-watering.
Quantitative parameters of tolerance measured include, but are not limited to, the average wet and dry weight, growth rate, leaf size, leaf coverage (overall leaf area), the weight of the seeds yielded, the average seed size and the number of seeds produced per plant. Transformed plants not exhibiting substantial physiological and/or morphological effects, or exhibiting higher biomass than wild-type plants, are identified as drought stress tolerant plants,
Salinity tolerance assay—Transgenic plants with tolerance to high salt concentrations are expected to exhibit better germination, seedling vigor or growth in high salt. Salt stress can be effected in many ways such as, for example, by irrigating the plants with a hyperosmotic solution, by cultivating the plants hydroponically in a hyperosmotic growth solution (e.g., Hoagland solution with added salt), or by culturing the plants in a hyperosmotic growth medium [e.g., 50% Murashige-Skoog medium (MS medium) with added salt]. Since different plants vary considerably in their tolerance to salinity, the salt concentration in the irrigation water, growth solution, or growth medium can be adjusted according to the specific characteristics of the specific plant cultivar or variety, so as to inflict a mild or moderate effect on the physiology and/or morphology of the plants (for guidelines as to appropriate concentration see, Bernstein and Kafkafi, Root Growth Under Salinity Stress In: Plant Roots, The Hidden Half 3rd ed. Waisel Y, Eshel A and Kafkafi U. (editors) Marcel Dekker Inc., New York, 2002, and reference therein).
For example, a salinity tolerance test can be performed by irrigating plants at different developmental stages with increasing concentrations of sodium chloride (for example 50 mM, 150 mM, 300 mM NaCl) applied from the bottom and from above to ensure even dispersal of salt. Following exposure to the stress condition the plants are frequently monitored until substantial physiological and/or morphological effects appear in wild type plants. Thus, the external phenotypic appearance, degree of chlorosis and overall success to reach maturity and yield progeny are compared between control and transgenic plants. Quantitative parameters of tolerance measured include, but are not limited to, the average wet and dry weight, growth rate, leaf size, leaf coverage (overall leaf area), the weight of the seeds yielded, the average seed size and the number of seeds produced per plant. Transformed plants not exhibiting substantial physiological and/or morphological effects, or exhibiting higher biomass than wild-type plants, are identified as abiotic stress tolerant plants.
Osmotic tolerance test—Osmotic stress assays (including sodium chloride and PEG assays) are conducted to determine if an osmotic stress phenotype was sodium chloride-specific or if it was a general osmotic stress related phenotype. Plants which are tolerant to osmotic stress may have more tolerance to drought and/or freezing. For salt and osmotic stress experiments, the medium is supplemented for example with 50 mM, 100 mM, 200 mM NaCl or 15%, 20% or 25% PEG.
Cold stress tolerance—One way to analyze cold stress is as follows. Mature (25 day old) plants are transferred to 4° C. chambers for 1 or 2 weeks, with constitutive light. Later on plants are moved back to greenhouse. Two weeks later damages from chilling period, resulting in growth retardation and other phenotypes, are compared between control and transgenic plants, by measuring plant weight (wet and dry), and by comparing growth rates measured as time to flowering, plant size, yield, and the like.
Heat stress tolerance—One way to measure heat stress tolerance is by exposing the plants to temperatures above 34° C. for a certain period. Plant tolerance is examined after transferring the plants back to 22° C. for recovery and evaluation after 5 days relative to internal controls (non-transgenic plants) or plants not exposed to neither cold or heat stress.
The biomass, vigor and yield of the plant can also be evaluated using any method known to one of ordinary skill in the art. Thus, for example, plant vigor can be calculated by the increase in growth parameters such as leaf area, fiber length, rosette diameter, plant fresh weight and the like per time.
As mentioned, the increase of plant yield can be determined by various parameters. For example, increased yield of rice may be manifested by an increase in one or more of the following: number of plants per growing area, number of panicles per plant, number of spikelets per panicle, number of flowers per panicle, increase in the seed filling rate, increase in thousand kernel weight (1000-weight), increase oil content per seed, increase starch content per seed, among others. An increase in yield may also result in modified architecture, or may occur because of modified architecture. Similarly, increased yield of soybean may be manifested by an increase in one or more of the following: number of plants per growing area, number of pods per plant, number of seeds per pod, increase in the seed filling rate, increase in thousand seed weight (1000-weight), reduce pod shattering, increase oil content per seed, increase protein content per seed, among others. An increase in yield may also result in modified architecture, or may occur because of modified architecture.
Thus, the present invention is of high agricultural value for increasing tolerance of plants to nitrogen deficiency or abiotic stress as well as promoting the yield, biomass and vigor of commercially desired crops.
According to another embodiment of the present invention, there is provided a food or feed comprising the plants or a portion thereof of the present invention.
In a further aspect the invention, the transgenic plants of the present invention or parts thereof are comprised in a food or feed product (e.g., dry, liquid, paste). A food or feed product is any ingestible preparation containing the transgenic plants, or parts thereof, of the present invention, or preparations made from these plants. Thus, the plants or preparations are suitable for human (or animal) consumption, i.e. the transgenic plants or parts thereof are more readily digested. Feed products of the present invention further include a oil or a beverage adapted for animal consumption.
It will be appreciated that the transgenic plants, or parts thereof, of the present invention may be used directly as feed products or alternatively may be incorporated or mixed with feed products for consumption. Furthermore, the food or feed products may be processed or used as is. Exemplary feed products comprising the transgenic plants, or parts thereof, include, but are not limited to, grains, cereals, such as oats, e.g. black oats, barley, wheat, rye, sorghum, corn, vegetables, leguminous plants, especially soybeans, root vegetables and cabbage, or green forage, such as grass or hay.
As used herein the term “about” refers to ±10%.
The terms “comprises”, “comprising”, “includes”, “including”, “having” and their conjugates mean “including but not limited to”.
The term “consisting of” means “including and limited to”.
The term “consisting essentially of” means that the composition, method or structure may include additional ingredients, steps and/or parts, but only if the additional ingredients, steps and/or parts do not materially alter the basic and novel characteristics of the claimed composition, method or structure.
As used herein, the singular form “a”, “an” and “the” include plural references unless the context clearly dictates otherwise. For example, the term “a compound” or “at least one compound” may include a plurality of compounds, including mixtures thereof.
Throughout this application, various embodiments of this invention may be presented in a range format. It should be understood that the description in range format is merely for convenience and brevity and should not be construed as an inflexible limitation on the scope of the invention. Accordingly, the description of a range should be considered to have specifically disclosed all the possible subranges as well as individual numerical values within that range. For example, description of a range such as from 1 to 6 should be considered to have specifically disclosed subranges such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6 etc., as well as individual numbers within that range, for example, 1, 2, 3, 4, 5, and 6. This applies regardless of the breadth of the range.
Whenever a numerical range is indicated herein, it is meant to include any cited numeral (fractional or integral) within the indicated range. The phrases “ranging/ranges between” a first indicate number and a second indicate number and “ranging/ranges from” a first indicate number “to” a second indicate number are used herein interchangeably and are meant to include the first and second indicated numbers and all the fractional and integral numerals therebetween.
As used herein the term “method” refers to manners, means, techniques and procedures for accomplishing a given task including, but not limited to, those manners, means, techniques and procedures either known to, or readily developed from known manners, means, techniques and procedures by practitioners of the chemical, pharmacological, biological, biochemical and medical arts.
As used herein, the term “treating” includes abrogating, substantially inhibiting, slowing or reversing the progression of a condition, substantially ameliorating clinical or aesthetical symptoms of a condition or substantially preventing the appearance of clinical or aesthetical symptoms of a condition.
It is appreciated that certain features of the invention, which are, for clarity, described in the context of separate embodiments, may also be provided in combination in a single embodiment. Conversely, various features of the invention, which are, for brevity, described in the context of a single embodiment, may also be provided separately or in any suitable subcombination or as suitable in any other described embodiment of the invention. Certain features described in the context of various embodiments are not to be considered essential features of those embodiments, unless the embodiment is inoperative without those elements.
Various embodiments and aspects of the present invention as delineated hereinabove and as claimed in the claims section below find experimental support in the following examples.
EXAMPLES
Reference is now made to the following examples, which together with the above descriptions illustrate some embodiments of the invention in a non limiting fashion.
Generally, the nomenclature used herein and the laboratory procedures utilized in the present invention include molecular, biochemical, microbiological and recombinant DNA techniques. Such techniques are thoroughly explained in the literature. See, for example, “Molecular Cloning: A laboratory Manual” Sambrook et al., (1989); “Current Protocols in Molecular Biology” Volumes I-III Ausubel, R. M., ed. (1994); Ausubel et al., “Current Protocols in Molecular Biology”, John Wiley and Sons, Baltimore, Md. (1989); Perbal, “A Practical Guide to Molecular Cloning”, John Wiley & Sons, New York (1988); Watson et al., “Recombinant DNA”, Scientific American Books, New York; Birren et al. (eds) “Genome Analysis: A Laboratory Manual Series”, Vols. 1-4, Cold Spring Harbor Laboratory Press, New York (1998); methodologies as set forth in U.S. Pat. Nos. 4,666,828; 4,683,202; 4,801,531; 5,192,659 and 5,272,057; “Cell Biology: A Laboratory Handbook”, Volumes I-III Cellis, J. E., ed. (1994); “Current Protocols in Immunology” Volumes I-III Coligan J. E., ed. (1994); Stites et al. (eds), “Basic and Clinical Immunology” (8th Edition), Appleton & Lange, Norwalk, Conn. (1994); Mishell and Shiigi (eds), “Selected Methods in Cellular Immunology”, W. H. Freeman and Co., New York (1980); available immunoassays are extensively described in the patent and scientific literature, see, for example, U.S. Pat. Nos. 3,791,932; 3,839,153; 3,850,752; 3,850,578; 3,853,987; 3,867,517; 3,879,262; 3,901,654; 3,935,074; 3,984,533; 3,996,345; 4,034,074; 4,098,876; 4,879,219; 5,011,771 and 5,281,521; “Oligonucleotide Synthesis” Gait, M. J., ed. (1984); “Nucleic Acid Hybridization” Hames, B. D., and Higgins S. J., eds. (1985); “Transcription and Translation” Hames, B. D., and Higgins S. J., Eds. (1984); “Animal Cell Culture” Freshney, R. I., ed. (1986); “Immobilized Cells and Enzymes” IRL Press, (1986); “A Practical Guide to Molecular Cloning” Perbal, B., (1984) and “Methods in Enzymology” Vol. 1-317, Academic Press; “PCR Protocols: A Guide To Methods And Applications”, Academic Press, San Diego, Calif. (1990); Marshak et al., “Strategies for Protein Purification and Characterization—A Laboratory Course Manual” CSHL Press (1996); all of which are incorporated by reference as if fully set forth herein. Other general references are provided throughout this document. The procedures therein are believed to be well known in the art and are provided for the convenience of the reader. All the information contained therein is incorporated herein by reference.
Example 1
Differential Expression of dsRNAs in Maize Plant Under Optimal Versus Deficient Nitrogen Conditions
Experimental Procedures
Plant Material
Corn seeds were obtained from Galil seeds (Israel). Corn variety 5605 was used in all experiments. Plants were grown at 24° C. under a 16 hr light:8 hr dark regime.
Stress Induction
Corn seeds were germinated and grown on agar with defined growth media containing either optimal (100% N2, 20.61 mM) or suboptimal nitrogen levels (1% or 10% N2, 0.2 mM or 2.06 mM, respectively). Seedlings aged one or two weeks were used for tissue samples for RNA analysis, as described below.
Total RNA Extraction
Total RNA of leaf or root samples from four to eight biological repeats were extracted using the mirVanaTM kit (Ambion, Austin, Tex.) by pooling 3-4 plants to one biological repeat.
Microarray Design
Custom microarrays were manufactured by Agilent Technologies by in situ synthesis. The first generation microarray consisted of a total of 13619 non-redundant DNA probes, the majority of which arose from deep sequencing data and includes different small RNA molecules (i.e. miRNAs, siRNA and predicted small RNA sequences), with each probe being printed once. An in-depth analysis of the first generation microarray, which included hybridization experiments as well as structure and orientation verifications on all its small RNAs, resulted in the formation of an improved, second generation, microarray. The second generation microarray consists of a total 4721 non-redundant DNA 45-nucleotide long probes for all known plant small RNAs, with 912 sequences (19.32%) from Sanger version 15 and the rest (3809), encompassing miRNAs (968=20.5%), siRNAs (1626=34.44%) and predicted small RNA sequences (1215=25.74%), from deep sequencing data accumulated by the inventors, with each probe being printed in triplicate.
Results
Wild type maize plants were allowed to grow at standard, optimal conditions or nitrogen deficient conditions for one or two weeks, at the end of which they were evaluated for NUE. Three to four plants from each group were used for reproducibility. Four to eight repeats were obtained for each group and RNA was extracted from leaf or root tissue. The expression level of the maize miRNAs was analyzed by high throughput microarray to identify miRNAs that were differentially expressed between the experimental groups.
Tables 1-4 below present dsRNA sequences that were found to be differentially expressed (upregulated=up; downregulated=down) in corn grown under low nitrogen conditions (nitrogen limiting conditions, as described above).







TABLE 1







miRNAs found upregulated in Plants Growing under Nitrogen


Deficient Conditions versus Optimal conditions














Stem

Fold
Fold




Loop SEQ

Change
Change


Mir Name
SEQ ID NO:
ID NO:
Direction
Leaf
Root















osa-miR1430
TGGTGAGCCTTCCTGGC
196
Up

3.99



TAAG/1






 


osa-miR1868
TCACGGAAAACGAGGG
197
Up

2.63



AGCAGCCA/2






 


osa-miR2096-3p
CCTGAGGGGAAATCGG
198
Up
3.48
2.71



CGGGA/3






 


zma-miR399f*
GGGCAACTTCTCCTTTG
199
Up

2.13



GCAGA/4






 


Predicted folded
AACTAAAACGAAACGG
200
Up
2.1



24-nts-long seq
AAGGAGTA/5






50935







 


Predicted folded
AAGGTGCTTTTAGGAGT
201
Up
2.08



24-nts-long seq
AGGACGG/6






51052







 


Predicted folded
ACAAAGGAATTAGAAC
202
Up
3.23
2.49


24-nts-long seq
GGAATGGC/7






51215







 


Predicted folded
AGAATCAGGAATGGAA
203
Up

1.54


24-nts-long seq
CGGCTCCG/8






51468







 


Predicted folded
AGAATCAGGGATGGAA
204
Up

1.9


24-nts-long seq
CGGCTCTA/9






51469







 


Predicted folded
AGAGTCACGGGCGAGA
205
Up

2.34


24-nts-long seq
AGAGGACG/10






51577







 


Predicted folded
AGGACCTAGATGAGCG
206
Up

1.72


24-nts-long seq
GGCGGTTT/11






51691







 


Predicted folded
AGGACGCTGCTGGAGA
207
Up

2.4


24-nts-long seq
CGGAGAAT/12






51695







 


Predicted folded
AGGGCTTGTTCGGTTTG
208
Up
2.52



24-nts-long seq
AAGGGGT/13






51814







 


Predicted folded
ATCTTTCAACGGCTGCG
209
Up

2.11


24-nts-long seq
AAGAAGG/14






52057







 


Predicted folded
CTAGAATTAGGGATGG
210
Up

1.57


24-nts-long seq
AACGGCTC/15






52327







 


Predicted folded
GAGGGATAACTGGGGA
211
Up
2.97



24-nts-long seq
CAACACGG/16






52499







 


Predicted folded
GCGGAGTGGGATGGGG
212
Up

1.51


24-nts-long seq
AGTGTTGC/17






52633







 


Predicted folded
GGAGACGGATGCGGAG
213
Up

1.51


24-nts-long seq
ACTGCTGG/18






52688







 


Predicted folded
GGTTAGGAGTGGATTG
214
Up
3.77



24-nts-long seq
AGGGGGAT/19






52805







 


Predicted folded
GTCAAGTGACTAAGAG
215
Up
4.93
10.17


24-nts-long seq
CATGTGGT/20






52850







 


Predicted folded
GTGGAATGGAGGAGAT
216
Up
2.01



24-nts-long seq
TGAGGGGA/21






52882







 


Predicted folded
TGGCTGAAGGCAGAAC
217
Up

4.45


24-nts-long seq
CAGGGGAG/22






53118







 


Predicted folded
TGTGGTAGAGAGGAAG
218
Up
3.25



24-nts-long seq
AACAGGAC/23






53149







 


Predicted folded
AGGGACTCTCTTTATTT
219
Up

1.83


24-nts-long seq
CCGACGG/24






53594







 


Predicted folded
AGGGTTCGTTTCCTGGG
220
Up

1.66


24-nts-long seq
AGCGCGG/25






53604







 


Predicted folded
TCCTAGAATCAGGGAT
221
Up

1.6


24-nts-long seq
GGAACGGC/26






54081







 


Predicted folded
TGGGAGCTCTCTGTTCG
222
Up

3.47


24-nts-long seq
ATGGCGC/27






54132







 


Predicted zma
AACGTCGTGTCGTGCTT
223
Up

1.62


mir 48061
GGGCT/28






 


Predicted zma
ACCTGGACCAATACAT
224
Up
2.58



mir 48295
GAGATT/29






 


Predicted zma
AGAAGCGACAATGGGA
225
Up
4.65



mir 48350
CGGAGT/30






 


Predicted zma
AGGAAGGAACAAACGA
226
Up

2.08


mir 48457
GGATAAG/31






 


Predicted zma
CCAAGAGATGGAAGGG
227
Up
2



mir 48877
CAGAGC/32






 


Predicted zma
CGACAACGGGACGGAG
228
Up

1.58


mir 48922
TTCAA/33






 


Predicted zma
GAGGATGGAGAGGTAC
229
Up
2.02



mir 49123
GTCAGA/34






 


Predicted zma
GATGGGTAGGAGAGCG
230
Up
1.51
1.55


mir 49161
TCGTGTG/35






 


Predicted zma
GATGGTTCATAGGTGA
231
Up

4.2


mir 49162
CGGTAG/36






 


Predicted zma
GGGAGCCGAGACATAG
232
Up

2.64


mir 49262
AGATGT/37






 


Predicted zma
GTGAGGAGTGATAATG
233
Up

2.17


mir 49323
AGACGG/38






 


Predicted zma
GTTTGGGGCTTTAGCAG
234
Up
1.58



mir 49369
GTTTAT/39






 


Predicted zma
TCCATAGCTGGGCGGA
235
Up

5.52


mir 49609
AGAGAT/40






 


Predicted zma
TCGGCATGTGTAGGAT
236
Up
3.24 ± 1.00 
3.235 ± 0.205


mir 49638
AGGTG/41






 


Predicted zma
TGATAGGCTGGGTGTG
237
Up
2.01
1.73


mir 49761
GAAGCG/42






 


Predicted zma
TGCAAACAGACTGGGG
238
Up

3


mir 49787
AGGCGA/43






 


Predicted zma
TTTGGCTGACAGGATA
239
Up
2.44



mir 50077
AGGGAG/44






 


Predicted zma
TTTTCATAGCTGGGCGG
240
Up
19.94



mir 50095
AAGAG/45






 


Predicted zma
AACTTTAAATAGGTAG
241
Up

1.51


mir 50110
GACGGCGC/46






 


Predicted zma
GGAATGTTGTCTGGTTC
242
Up
14.34



mir 50204
AAGG/47






 


Predicted zma
TGTAATGTTCGCGGAA
243
Up

1.7


mir 50261
GGCCAC/48






 


Predicted zma
TGTTGGCATGGCTCAAT
244
Up

1.82


mir 50267
CAAC/49






 


Predicted zma
CGCTGACGCCGTGCCA
245
Up

2.33


mir 50460
CCTCAT/50






 


Predicted zma
GCCTGGGCCTCTTTAGA
246
Up

1.5


mir 50545
CCT/51






 


Predicted zma
GTAGGATGGATGGAGA
247
Up

2.07


mir 50578
GGGTTC/52






 


Predicted zma
TCAACGGGCTGGCGGA
248
Up

1.55


mir 50611
TGTG/53





Table 1. provided are miRNAs that were found upregulated in Plants Growing under Nitrogen Deficient Conditions versus Optimal conditions.













TABLE 2







miRNAs found downregulated in Plants Growing under Nitrogen


Deficient Conditions versus Optimal conditions














Stem Loop

Fold
Fold



Mature
Sequence/

Change
Change


Mir Name
Sequence/seq id no:
seq id no:
Direction
Leaf
Root















aqc-
AGAAGAGAGAG
249
Down
1.53



miR529
AGCACAACCC/54






 


ath-
CTTGAGAGAGAG
250
Down
1.54



miR2936
AACACAGACG/55






 


mtr-
TGAGCCAGGATG
251
Down
3.04



miR169q
ACTTGCCGG/56






 


peu-
GGCCGGGGGACG
254
Down
1.66



miR2911
GGCTGGGA/59






 


Predicted
AAAAAAGACTGA
255
Down

2.66


folded 24-
GCCGAATTGAAA/






nts-long
60






seq 50703







 


Predicted
AAGGAGTTTAAT
256
Down
1.62



folded 24-
GAAGAAAGAGA






nts-long
G/61






seq 51022







 


Predicted
ACTGATGACGAC
257
Down
7.7



folded 24-
ACTGAGGAGGCT/






nts-long
62






seq 51381







 


Predicted
AGAGGAACCAGA
258
Down
1.52



folded 24-
GCCGAAGCCGTT/






nts-long
63






seq 51542







 


Predicted
AGGCAAGGTGGA
259
Down
2.07



folded 24-
GGACGTTGATGA/






nts-long
64






seq 51757







 


Predicted
AGGGCTGATTTG
260
Down
3.7
2.04


folded 24-
GTGACAAGGGGA/






nts-long
65






seq 51802







 


Predicted
ATATAAAGGGAG






folded 24-
GAGGTATGGACC/
261
Down
2.1



nts-long
66






seq 51966







 


Predicted
ATCGGTCAGCTG
262
Down
1.7



folded 24-
GAGGAGACAGGT/






nts-long
67






seq 52041







 


Predicted
ATGGTAAGAGAC
263
Down

1.62


folded 24-
TATGATCCAACT/






nts-long
68






seq 52109







 


Predicted
CAATTTTGTACT
264
Down

1.53


folded 24-
GGATCGGGGCAT/






nts-long
69






seq 52212







 


Predicted
CAGAGGAACCAG
265
Down
1.58



folded 24-
AGCCGAAGCCGT/






nts-long
70






seq 52218







 


Predicted
CGGCTGGACAGG
266
Down
1.63



folded 24-
GAAGAAGAGCAC/






nts-long
71






seq 52299







 


Predicted
GAAACTTGGAGA
267
Down

1.7


folded 24-
GATGGAGGCTTT/






nts-long
72






seq 52347







 


Predicted
GAGAGAGAAGG
268
Down
3.25
2.52


folded 24-
GAGCGGATCTGG






nts-long
T/73






seq 52452







 


Predicted
GCTGCACGGGAT
269
Down
2.34



folded 24-
TGGTGGAGAGGT/






nts-long
74






seq 52648







 


Predicted
GGCTGCTGGAGA
270
Down
2.13



folded 24-
GCGTAGAGGACC/






nts-long
75






seq 52739







 


Predicted
GGGTTTTGAGAG
271
Down

2.9


folded 24-
CGAGTGAAGGGG/






nts-long
76






seq 52792







 


Predicted
GGTATTGGGGTG
272
Down
1.59



folded 24-
GATTGAGGTGGA/






nts-long
77






seq 52795







 


Predicted
GGTGGCGATGCA
273
Down
2.52
3.87


folded 24-
AGAGGAGCTCAA/






nts-long
78






seq 52801







 


Predicted
GTTGCTGGAGAG
274
Down

2.35


folded 24-
AGTAGAGGACGT/






nts-long
79






seq 52955







 


Predicted
AAAAGAGAAACC
275
Down

1.78


zma mir
GAAGACACAT/80






47944







 


Predicted
AAAGAGGATGAG
276
Down
4.09



zma mir
GAGTAGCATG/81






47976







 


Predicted
AATACACATGGG
277
Down
1.85



zma mir
TTGAGGAGG/82






48185







 


Predicted
AGAAGCGGACTG
278
Down
3.18



zma mir
CCAAGGAGGC/83






48351







 


Predicted
AGAGGGTTTGGG
279
Down

8.95


zma mir
GATAGAGGGAC/84






48397







 


Predicted
TAAGGGATGAGG
280
Down

2.1


zma mir
CAGAGCATG/85






48588







 


Predicted
ATGCTATTTGTA
281
Down

1.67


zma mir
CCCGTCACCG/86






48669







 


Predicted
ATGTGGATAAAA
282
Down

1.61


zma mir
GGAGGGATGA/87






48708







 


Predicted
CAACAGGAACAA
283
Down
1.52



zma mir
GGAGGACCAT/88






48771







 


Predicted
CTCGAGTTGAGAA
284
Down

1.51


zma mir
AGAGATGCT/89






49002







 


Predicted
CTCGATGGGAGGT
285
Down
1.61



zma mir
GGAGTTGCAT/90






49003







 


Predicted
CTGGGAAGATGG
286
Down

1.64


zma mir
AACATTTTGGT/91






49011







 


Predicted
GAAGATATACGA
287
Down
1.55



zma mir
TGATGAGGAG/92






49053







 


Predicted
GAATCTATCGTT
288
Down
1.65
2.01


zma mir
TGGGCTCAT/93






49070







 


Predicted
GAGCGAGCTACAA
289
Down
1.6



zma mir
AAGGATTCG/94






49082







 


Predicted
GAGTGACGAGGAG
290
Down

3.64


zma mir
TGAGAGTAGG/95






49155







 


Predicted
GGGCATCTTCTG
291
Down
1.64



zma mir
GCAGGAGGACA/






49269
96






 


Predicted
TACGGAAGAAGA
292
Down
1.64



zma mir
GCAAGTTTT/97






49435







 


Predicted
TAGAAAGAGCGA
293
Down

1.55


zma mir
GAGAACAAAG/98






49445







 


Predicted
TGATATTATGGA
294
Down
1.54
1.57


zma mir
CGACTGGTT/99






49762







 


Predicted
TGGAAGGGCCAT
295
Down

2.45


zma mir
GCCGAGGAG/100






49816







 


Predicted
TTGAGCGCAGCG
296
Down

2.93


zma mir
TTGATGAGC/101






49985







 


Predicted
TTGGATAACGGG
297
Down

1.79


zma mir
TAGTTTGGAGT/






50021
102






 


Predicted
AGCTGCCGACTC
298
Down

1.54


zma mir
ATTCACCCA/103






50144







 


Predicted
TGTACGATGATC
299
Down
1.53



zma mir
AGGAGGAGGT/






50263
104






 


Predicted
TGTGTTCTCAGG
300
Down

2.51


zma mir
TCGCCCCCG/105






50266







 


Predicted
ACTAAAAAGAAA
301
Down
1.5



zma mir
CAGAGGGAG/106






50318







 


Predicted
GACCGGCTCGAC
302
Down
1.55



zma mir
CCTTCTGC/107






50517







 


Predicted
TGGTAGGATGGA
303
Down
1.55



zma mir
TGGAGAGGGT/






50670
108






 


zma-
GGAATGTTGTCT
304
Down
1.73



miR166d*
GGTTCAAGG/109






 


zma-
GGCAAGTCTGTC
305
Down
2.41



miRl69c*
CTTGGCTACA/110






 


zma-
TGCCAAAGGGGA
309
Down

1.55


miR399g
TTTGCCCGG/113





Table 2. provided are miRNAs that were found downregulated in Plants Growing under Nitrogen Deficient Conditions versus Optimal conditions.













TABLE 3







siRNAs found upregulated in Plants Growing under Nitrogen Deficient


Conditions versus Optimal conditions














Fold
Fold Change


Mir Name
Mature Sequence/SEQ ID NO:
Direction
Change Leaf
Root














Predicted
AAGAAACGGGGCAGTGAGA
Up

1.51


siRNA 54339
TGGAC/114





 


Predicted
AGAAAAGATTGAGCCGAAT
Up
2.02



siRNA 54631
TGAATT/115





 


Predicted
AGAGCCTGTAGCTAATGGT
Up
1.95



siRNA 54991
GGG/116





 


Predicted
AGGTAGCGGCCTAAGAACG
Up
2.36
1.67


siRNA 55111
ACACA/117





 


Predicted
CCTATATACTGGAACGGAA
Up

1.57


siRNA 55423
CGGCT/118





 


Predicted
CTATATACTGGAACGGAAC
Up

2.23


siRNA 55806
GGCTT/119





 


Predicted
GACGAGATCGAGTCTGGAG
Up
1.86



siRNA 56052
CGAGC/120





 


Predicted
GAGTATGGGGAGGGACTAG
Up

2.3


siRNA 56106
GGA/121





 


Predicted
GACGAAATAGAGGCTCAGG
Up
2.08



siRNA 56353
AGAGG/122





 


Predicted
GGATTCGTGATTGGCGATG
Up

1.51


siRNA 56388
GGG/123





 


Predicted
GGTGAGAAACGGAAAGGCA
Up
4.04



siRNA 56406
GGACA/124





 


Predicted
GTGTCTGAGCAGGGTGAGA
Up
1.53
1.58


siRNA 56443
AGGCT/125





 


Predicted
GTTTTGGAGGCGTAGGCGA
Up
3.04



siRNA 56450
GGGAT/126





 


Predicted
TGGGACGCTGCATCTGTTGA
Up
2.96



siRNA 56542
T/127





 


Predicted
TCTATATACTGGAACGGAA
Up

1.76


siRNA 56706
CGGCT/128





 


Predicted
GTTGTTGGAGGGGTAGAGG
Up
1.55



siRNA 56856
ACGTC/129





 


Predicted
AATGACAGGACGGGATGGG
Up

2.87


siRNA 57034
ACGGG/130





 


Predicted
ACGGAACGGCTTCATACCA
Up

2.43


siRNA 57054
CAATA/131





 


Predicted
GACGGGCCGACATTTAGAG
Up

1.69


siRNA 57193
CACGG/132





 


Predicted
ACGGATAAAAGGTACTCT/
Up

2.82


siRNA 57884
133





 


Predicted
AGTATGTCGAAAACTGGAG
Up
4.54



siRNA 58292
GGC/134





 


Predicted
ATAAGCACCGGCTAACTCT/
Up

2.87


siRNA 58362
135





 


Predicted
ATTCAGCGGGCGTGGTTATT
Up

1.55


siRNA 58665
GGCA/136





 


Predicted
CAGCGGGTGCCATAGTCGA
Up

1.92


siRNA 58872
T/137





 


Predicted
CATTGCGACGGTCCTCAA/
Up

1.57


siRNA 58940
138





 


Predicted
CTCAACGGATAAAAGGTAC/
Up

2.21


siRNA 59380
139





 


Predicted
GACAGTCAGGATGTTGGCT/
Up
2.68
2.12


siRNA 59626
140





 


Predicted
GACTGATCCTTCGGTGTCGG
Up

1.67


siRNA 59659
CG/141





 


Predicted
GCCGAAGATTAAAAGACGA
Up
1.64



siRNA 59846
GACGA/142





 


Predicted
GCCTTTGCCGACCATCCTGA/
Up

1.6


siRNA 59867
143





 


Predicted
GGAATCGCTAGTAATCGTG
Up
1.87
1.76


siRNA 59952
GAT/144





 


Predicted
GGAGCAGCTCTGGTCGTGG
Up

1.85 ± 0.007


siRNA 59961
G/145





 


Predicted
GGAGGCTCGACTATGTTCA
Up

2.97


siRNA 59965
AA/146





 


Predicted
GGAGGGATGTGAGAACATG
Up

1.62


siRNA 59966
GGC/147





 


Predicted
GTCCCCTTCGTCTAGAGGC/
Up

2.82


siRNA 60081
148





 


Predicted
GTCTGAGTGGTGTAGTTGGT/
Up
2.12



siRNA 60095
149





 


Predicted
GTTGGTAGAGCAGTTGGC/
Up

4.11


siRNA 60188
150





 


Predicted
TACGTTCCCGGGTCTTGTAC
Up

1.95


siRNA 60285
A/151





 


Predicted
TATGGATGAAGATGGGGGT
Up
3.68



siRNA 60387
G/152





 


Predicted
TCAACGGATAAAAGGTACT
Up

2.23


siRNA 60434
CCG/153





 


Predicted
TGCCCAGTGCTTTGAATG/
Up

3.37


siRNA 60837
154





 


Predicted
TGCGAGACCGACAAGTCGA
Up
1.64
1.86


siRNA 60850
GC/155





 


Predicted
TTTGCGACACGGGCTGCTCT/
Up

1.52


siRNA 61382
156





Table 3. Provided are siRNAs that were found upregulated in Plants Growing under Nitrogen Deficient Conditions versus Optimal conditions.













TABLE 4







siRNAs found downregulated in Plants Growing under Nitrogen Deficient


Conditions versus Optimal conditions














Fold
Fold





Change
Change


Mir Name
Mature Sequence/SEQ ID No:
Direction
Leaf
Root














Predicted siRNA
CATCGCTCAACGGACAAAAG
Down

1.55


58924
GT/157





 


Predicted siRNA
AAGACGAAGGTAGCAGCGC
Down
2.79



54240
GATAT/158





 


Predicted siRNA
AGCCAGACTGATGAGAGAA
Down
1.51



54957
GGAGG/159





 


Predicted siRNA
ACGTTGTTGGAAGGGTAGAG
Down
1.56



55081
GACG/160





 


Predicted siRNA
CAAGTTATGCAGTTGCTGCC
Down

5.98


55393
T/161





 


Predicted siRNA
CAGAATGGAGGAAGAGATG
Down
3.49



55404
GTG/162





 


Predicted siRNA
ATCTGTGGAGAGAGAAGGTT
Down
1.58



55472
GCCC/163





 


Predicted siRNA
ATGTCAGGGGGCCATGCAGT
Down
2.41



55720
AT/164





 


Predicted siRNA
ATCCTGACTGTGCCGGGCCG
Down
1.96



55732
GCCC/165





 


Predicted siRNA
CGAGTTCGCCGTAGAGAAAG
Down

2.24


56034
CT/166





 


Predicted siRNA
GACTGATTCGGACGAAGGAG
Down

3.23


56162
GGTT/167





 


Predicted siRNA
GTCTGAACACTAAACGAAGC
Down
1.87



56205
ACA/168





 


Predicted siRNA
GACGTTGTTGGAAGGGTAGA
Down
3.94



56277
GGAC/169





 


Predicted siRNA
GCTACTGTAGTTCACGGGCC
Down
1.71



56307
GGCC/170





 


Predicted siRNA
GGTATTCGTGAGCCTGTTTCT
Down
1.67



56425
GGTT/171





 


Predicted siRNA
TGGAAGGAGCATGCATCTTG
Down

2.68


56837
AG/172





 


Predicted siRNA
TTCTTGACCTTGTAAGACCC
Down

3.66


56965
A/173





 


Predicted siRNA
AGCAGAATGGAGGAAGAGA
Down
1.53



57088
TGG/174





 


Predicted siRNA
CTGGACACTGTTGCAGAAGG
Down
1.58



57179
AGGA/175





 


Predicted siRNA
GAAATAGGATAGGAGGAGG
Down
3.34
2.91


57181
GATGA/176





 


Predicted siRNA
GGCACGACTAACAGACTCAC
Down

2.45


57228
GGGC/177





 


Predicted siRNA
AATCCCGGTGGAACCTCCA/
Down
3.6
2.7


57685
178





 


Predicted siRNA
ACACGACAAGACGAATGAG
Down

1.57


57772
AGAGA/179





 


Predicted siRNA
ACGACGAGGACTTCGAGACG/
Down
1.53



57863
180





 


Predicted siRNA
CAAAGTGGTCGTGCCGGAG/
Down
1.61



58721
181





 


Predicted siRNA
CAGCTTGAGAATCGGGCCGC/
Down
3.8



58877
182





 


Predicted siRNA
CCCTGTGACAAGAGGAGGA/
Down
1.6



59032
183





 


Predicted siRNA
CCTGCTAACTAGTTATGCGG
Down
1.74



59102
AGC/184





 


Predicted siRNA
CGAACTCAGAAGTGAAACC/
Down
2.11
2.62


59123
185





 


Predicted siRNA
CGCTTCGTCAAGGAGAAGGG
Down
1.59



59235
C/186





 


Predicted siRNA
CTTAACTGGGCGTTAAGTTG
Down

2.17


59485
CAGGGT/187





 


Predicted siRNA
GGACGAACCTCTGGTGTACC/
Down

1.76


59954
188





 


Predicted siRNA
GGCGCTGGAGAACTGAGGG/
Down

2.58


59993
189





 


Predicted siRNA
GGGGGCCTAAATAAAGACT/
Down
2.48



60012
190





 


Predicted siRNA
GTGCTAACGTCCGTCGTGAA/
Down

3.15


60123
191





 


Predicted siRNA
TAGCTTAACCTTCGGGAGGG/
Down

1.9


60334
192





 


Predicted siRNA
TGAGAAAGAAAGAGAAGGC
Down
1.64



60750
TCA/193





 


Predicted siRNA
TGATGTCCTTAGATGTTCTG
Down

1.99


60803
GGC/194





 


Predicted siRNA
CATGTGTTCTCAGGTCGCCC
Down

2.55


55413
C/195





Table 4. Provided are siRNAs that were found downregulated in Plants Growing under Nitrogen Deficient Conditions versus Optimal conditions.






Example 2
Target Prediction Using Bioinformatics Tools
A high throughput screening was performed on microarrays loaded with miRNAs/siRNAs that were found to be differentially expressed under multiple stress and optimal environmental conditions and in different plant tissues. The initial trait-associated miRNAs are later validated by quantitative Real Time PCR (qRT-PCR).
Target prediction—orthologous genes to the genes of interest in maize and/or Arabidopsis are found through a proprietary tool that analyzes publicly available genomic as well as expression and gene annotation databases from multiple plant species. Homologous as well as orthologous protein and nucleotide sequences of target genes of the small RNA sequences of the invention, were found using BLAST having at least 70% identity on at least 60% of the entire master gene length, and are summarized in Tables 5-8 below. BLAST version used was Version 2.2.25+, Released March 2011, at default parameters as follows: For step 1 using BlastX to find the master homolog: Word size 3, Gap open 11, gap extend 1. For step 2 using BlastN to find orthologs from other organisms: Word size 28, Gap open 0, Gap extend 0, Reward (match score) 1, Penalty (mismatch score)−2.







TABLE 5







Target Genes of miRNAs Associated with Increased NUE (Table 1)


















p.p.




Mir
Hom.


SEQ
p.n.


Mir
Bind.
NCBI


ID
SEQ ID


name
Pos.
Accession
Annotation/Organism
Identity
NO:
NO:
















osa-
 95-115
XP_002468137
hypothetical protein
1.00
311
515


miR2096-


SORBIDRAFT_01g040220


3p


[Sorghum bicolor]





> gi|241921991|gb|EER95135.1|





hypothetical protein





SORBIDRAFT_01g040220





[Sorghum bicolor]




ACN26598
unknown [Zea mays]
0.95
312
516




NP_001148956
LOC100282576 [Zea mays]
0.95
313
517





> gi|195623616|gb|ACG33638.1|





CONSTANS





interacting protein 4 [Zea





mays]




ACG37488
CONSTANS interacting
0.96
314
518





protein 4 [Zea mays]




NP_001148721
CONSTANS interacting
0.96
315
519





protein 4 [Zea mays]





> gi|195621640|gb|ACG32650.1|





CONSTANS





interacting protein 4 [Zea





mays]




NP_001049637
Os03g0263800 [Oryza sativa
0.89
316
520





Japonica Group]





> gi|29893607|gb|AAP06861.1|





unknown protein [Oryza





sativa Japonica Group]





> gi|108707320|gb|ABF95115.1|





S-ribonuclease binding





protein SBP1, putative,





expressed [Oryza sativa





Japonica Group]





> gi|113548108|dbj|BAF11551.1|





Os03g0263800 [Oryza





sativa Japonica Group]





> gi|215692696|dbj|BAG88116.1|





unnamed protein





product [Oryza sativa





Japonica Group]





> gi|215704492|dbj|BAG93926.1|





unnamed protein





product [Oryza sativa





Japonica Group]





> gi|222624614|gb|EEE58746.1|





hypothetical protein





OsJ_10235 [Oryza sativa





Japonica Group]




EEC74912
hypothetical protein
0.88
317





OsI_10851 [Oryza sativa





Indica Group]




BAJ94154
predicted protein [Hordeum
0.85
318
521





vulgare subsp. vulgare]





> gi|326493392|dbj|BAJ85157.1|





predicted protein





[Hordeum vulgare subsp.





vulgare]


Predicted
292-314
NP_001169860
hypothetical protein
1.00
319
522


zma


LOC100383754 [Zea mays]


mir


> gi|224032063|gb|ACN35107.1|


49161


unknown [Zea mays]




XP_002458357
hypothetical protein
0.88
320
523





SORBIDRAFT_03g031980





[Sorghum bicolor]





> gi|241930332|gb|EES03477.1|





hypothetical protein





SORBIDRAFT_03g031980





[Sorghum bicolor]



282-304
ACN34890
unknown [Zea mays]
1.00
321
524




EEC71328
hypothetical protein
0.74
322





OsI_03374 [Oryza sativa





Indica Group]





> gi|222619103|gb|EEE55235.1|





hypothetical protein





OsJ_03112 [Oryza sativa





Japonica Group]




BAD81811
hypothetical protein [Oryza
0.74
323
525





sativa Japonica Group]




BAJ90295
predicted protein [Hordeum
0.74
324
526





vulgare subsp. vulgare]



353-375
XP_002458357
hypothetical protein
1.00
325
527





SORBIDRAFT_03g031980





[Sorghum bicolor]





> gi|241930332|gb|EES03477.1|





hypothetical protein





SORBIDRAFT_03g031980





[Sorghum bicolor]




NP_001169860
hypothetical protein
0.88
326
528





LOC100383754 [Zea mays]





> gi|224032063|gb|ACN35107.1|





unknown [Zea mays]


Predicted
 982-1003
XP_002446326
hypothetical protein
1.00
327
529


zma


SORBIDRAFT_06g014320


mir


[Sorghum bicolor]


50460


> gi|241937509|gb|EES10654.1|





hypothetical protein





SORBIDRAFT_06g014320





[Sorghum bicolor]




NP_001169348
hypothetical protein
0.84
328
530





LOC100383215 [Zea mays]





> gi|224028855|gb|ACN33503.1|





unknown [Zea mays]




ACN25775
unknown [Zea mays]
0.75
329
531



426-447
NP_001136483
hypothetical protein
1.00
330
532





LOC100216597 [Zea mays]





> gi|194695886|gb|ACF82027.1|





unknown [Zea mays]



295-316
ACG40990
RING-H2 finger protein
1.00
331
533





ATL5I [Zea mays]




NP_001159129
hypothetical protein
0.85
332
534





LOC100304207 [Zea mays]





> gi|223942155|gb|ACN25161.1|





unknown [Zea mays]




NP_001130949
hypothetical protein
0.77
333
535





LOC100192054 [Zea mays]





> gi|194690534|gb|ACF79351.1|





unknown [Zea mays]




BAJ92353
predicted protein [Hordeum
0.76
334
536





vulgare subsp. vulgare]




NP_001057231
Os06g0233200 [Oryza sativa
0.76
335
537





Japonica Group]





> gi|51535192|dbj|BAD38165.1|





putative RING finger 1





[Oryza sativa Japonica





Group]





> gi|113595271|dbj|BAF19145.1|





Os06g0233200 [Oryza





sativa Japonica Group]





> gi|125554660|gb|EAZ00266.1|





hypothetical protein





OsI_22277 [Oryza sativa





Indica Group]





> gi|215766038|dbj|BAG98266.1|





unnamed protein





product [Oryza sativa





Japonica Group]





> gi|222635260|gb|EEE65392.1|





hypothetical protein





OsJ_20714 [Oryza sativa





Japonica Group]



154-175
NP_001183617
hypothetical protein
1.00
336
538





LOC100502211 [Zea mays]





> gi|238013474|gb|ACR37772.1|





unknown [Zea mays]




XP_002465702
hypothetical protein
0.73
337
539





SORBIDRAFT_01g044080





[Sorghum bicolor]





> gi|241919556|gb|EER92700.1|





hypothetical protein





SORBIDRAFT_01g044080





[Sorghum bicolor]



 93-114
NP_001152266
BHLH transcription factor
1.00
338
540





[Zea mays]





> gi|195654447|gb|ACG46691.1|





BHLH transcription





factor [Zea mays]



1159-1180
ACN25775
unknown [Zea mays]
1.00
339
541




NP_001169348
hypothetical protein
1.00
340
542





LOC100383215 [Zea mays]





> gi|224028855|gb|ACN33503.1|





unknown [Zea mays]




XP_002446326
hypothetical protein
0.80
341
543





SORBIDRAFT_06g014320





[Sorghum bicolor]





> gi|241937509|gb|EES10654.1|





hypothetical protein





SORBIDRAFT_06g014320





[Sorghum bicolor]


Predicted
1984-2007
XP_002444807
hypothetical protein
1.00
342
544


folded


SORBIDRAFT_07g028330


24-nts-


[Sorghum bicolor]


long


> gi|241941157|gb|EES14302.1|


seq


hypothetical protein


51695


SORBIDRAFT_07g028330





[Sorghum bicolor]




NP_001169681
hypothetical protein
0.90
343
545





LOC100383562 [Zea mays]





> gi|224030801|gb|ACN34476.1|





unknown [Zea mays]





> gi|224030845|gb|ACN34498.1|





unknown [Zea mays]


Predicted
584-606
NP_001161741
hypothetical protein
1.00
344
546


zma


LOC100192929 isoform 3


mir


[Zea mays]


48457


> gi|226713359|sp|B6UGG4.1|





NNJA4_MAIZE





RecName: Full = Ninja-family





protein 4





> gi|195657959|gb|ACG48447.1|





hypothetical protein





[Zea mays]




NP_001161740
hypothetical protein
1.00
345
547





LOC100192929 isoform 2





[Zea mays]





> gi|226713259|sp|B4FM28.1|





NNJA2_MAIZE





RecName: Full = Ninja-family





protein 2





> gi|194698174|gb|ACF83171.1|





unknown [Zea mays]




B6SLJ0
RecName: Full = Ninja-family
0.98
346





protein 3





> gi|195607786|gb|ACG25723.1|





hypothetical protein





[Zea mays]




ACN33721
unknown [Zea mays]
0.97
347
548



62-84
B6SLJ0
RecName: Full = Ninja-family
1.00
348





protein 3





> gi|195607786|gb|ACG25723.1|





hypothetical protein





[Zea mays]




NP_001161740
hypothetical protein
0.98
349
549





LOC100192929 isoform 2





[Zea mays]





> gi|226713259|sp|B4FM28.1|





NNJA2_MAIZE





RecName: Full = Ninja-family





protein 2





> gi|194698174|gb|ACF83171.1|





unknown [Zea mays]




ACN33721
unknown [Zea mays]
0.95
350
550




NP_001161741
hypothetical protein
0.97
351
551





LOC100192929 isoform 3





[Zea mays]





> gi|226713359|sp|B6UGG4.1|





NNJA4_MAIZE





RecName: Full = Ninja-family





protein 4





> gi|195657959|gb|ACG48447.1|





hypothetical protein





[Zea mays]



543-565
NP_001161740
hypothetical protein
1.00
352
552





LOC100192929 isoform 2





[Zea mays]





> gi|226713259|sp|B4FM28.1|





NNJA2_MAIZE





RecName: Full = Ninja-family





protein 2





> gi|194698174|gb|ACF83171.1|





unknown [Zea mays]




ACN33721
unknown [Zea mays]
0.97
353
553




NP_001161741
hypothetical protein
0.99
354
554





LOC100192929 isoform 3





[Zea mays]





> gi|226713359|sp|B6UGG4.1|





NNJA4_MAIZE





RecName: Full = Ninja-family





protein 4





> gi|195657959|gb|ACG48447.1|





hypothetical protein





[Zea mays]




B6SLJ0
RecName: Full = Ninja-family
0.98
355





protein 3





> gi|195607786|gb|ACG25723.1|





hypothetical protein





[Zea mays]



450-472
NP_001131584
hypothetical protein
1.00
356
555





LOC100192929 isoform 1





[Zea mays]





> gi|226713190|sp|B4FAF3.1|





NNJA1_MAIZE RecName:





Full = Ninja-family protein 1





> gi|194690024|gb|ACF79096.1|





unknown [Zea mays]





> gi|194691932|gb|ACF80050.1|





unknown [Zea mays]





> gi|195638284|gb|ACG38610.1|





hypothetical protein





[Zea mays]




XP_002465644
hypothetical protein
0.83
357
556





SORBIDRAFT_01g042880





[Sorghum bicolor]





> gi|241919498|gb|EER92642.1|





hypothetical protein





SORBIDRAFT_01g042880





[Sorghum bicolor]


Predicted
1313-1334
NP_001182893
hypothetical protein
1.00
358
557


zma


LOC100501172 [Zea mays]


mir


> gi|238008020|gb|ACR35045.1|


50261


unknown [Zea mays]


Predicted
 86-109
XP_002447337
hypothetical protein
1.00
359
558


folded


SORBIDRAFT_06g033160


24-nts-


[Sorghum bicolor]


long


> gi|241938520|gb|EES11665.1|


seq


hypothetical protein


52805


SORBIDRAFT_06g033160





[Sorghum bicolor]




NP_001142056
hypothetical protein
0.96
360
559





LOC100274212 [Zea mays]





> gi|194706940|gb|ACF87554.1|





unknown [Zea mays]





> gi|223947485|gb|ACN27826.1|





unknown [Zea mays]




ACG45259
hypothetical protein [Zea
0.96
361
560





mays]




EEC78262
hypothetical protein
0.86
362





OsI_17948 [Oryza sativa





Indica Group]




EEE61915
hypothetical protein
0.86
363





OsJ_16648 [Oryza sativa





Japonica Group]




BAJ96591
predicted protein [Hordeum
0.86
364
561





vulgare subsp. vulgare]




CAJ86266
H0901F07.3 [Oryza sativa
0.76
365
562





Indica Group]



596-619
AAY57857
cysteine proteinase inhibitor
1.00
366
563





[Zea mays subsp.





parviglumis]





> gi|66866419|gb|AAY57858.1|





cysteine proteinase





inhibitor [Zea mays subsp.





parviglumis]





> gi|66866423|gb|AAY57860.1|





cysteine proteinase





inhibitor [Zea mays subsp.





parviglumis]





> gi|66866425|gb|AAY57861.1|





cysteine proteinase





inhibitor [Zea mays subsp.





parviglumis]





> gi|66866427|gb|AAY57862.1|





cysteine proteinase





inhibitor [Zea mays subsp.





parviglumis]





> gi|66866429|gb|AAY57863.1|





cysteine proteinase





inhibitor [Zea mays subsp.





parviglumis]





> gi|66866435|gb|AAY57866.1|





cysteine proteinase





inhibitor [Zea mays subsp.





parviglumis]




AAY57867
cysteine proteinase inhibitor
0.99
367
564





[Zea mays subsp.





parviglumis]




ABQ32295
cysteine protease inhibitor
0.99
368
565





[Zea mays]




AAY57864
cysteine proteinase inhibitor
0.96
369
566





[Zea mays subsp.





parviglumis]





> gi|66866439|gb|AAY57868.1|





cysteine proteinase





inhibitor [Zea mays subsp.





parviglumis]




AAY57859
cysteine proteinase inhibitor
0.98
370
567





[Zea mays subsp.





parviglumis]




BAA09666
cysteine proteinase inhibitor
0.95
371
568





[Zea mays]





> gi|66866433|gb|AAY57865.1|





cysteine proteinase





inhibitor [Zea mays subsp.





parviglumis]





> gi|71794635|emb|CAJ20024.1|





putative cystatin [Zea





mays]




CAA60610
cysteine proteinase inhibitor
0.94
372
569





[Zea mays]




NP_001106013
cystatin2 [Zea mays]
0.94
373
570





> gi|1008922|dbj|BAA07327.1|





cystatin II [Zea mays]




BAB21558
cystatin [Coix lacryma-jobi]
0.86
374
571




NP_001105295
cystatin-1 precursor [Zea
0.84
375
572





mays]





> gi|399334|sp|P31726.1|CYT1_MAIZE





RecName:





Full = Cystatin-1; AltName:





Full = Corn kernel cysteine





proteinase inhibitor;





AltName: Full = Cystatin I;





Flags: Precursor





> gi|217962|dbj|BAA01472.1|





corn cystatin I [Zea mays]



2324-2347
BAJ85758
predicted protein [Hordeum
1.00
376
573





vulgare subsp. vulgare]




NP_001044791
Os01g0846600 [Oryza sativa
0.81
377
574





Japonica Group]





> gi|15408875|dbj|BAB64266.1|





ankyrin-like protein





[Oryza sativa Japonica





Group]





> gi|20160625|dbj|BAB89571.1|





ankyrin-like protein





[Oryza sativa Japonica





Group]





> gi|113534322|dbj|BAF06705.1|





Os01g0846600 [Oryza





sativa Japonica Group]





> gi|215687255|dbj|BAG91820.1|





unnamed protein





product [Oryza sativa





Japonica Group]





> gi|222619533|gb|EEE55665.1|





hypothetical protein





OsJ_04065 [Oryza sativa





Japonica Group]




XP_002458754
hypothetical protein
0.81
378
575





SORBIDRAFT_03g039680





[Sorghum bicolor]





> gi|241930729|gb|EES03874.1|





hypothetical protein





SORBIDRAFT_03g039680





[Sorghum bicolor]




ACR35053
unknown [Zea mays]
0.81
379
576




NP_001147927
LOC100281537 [Zea mays]
0.79
380
577





> gi|195614640|gb|ACG29150.1|





ankyrin-like protein





[Zea mays]


Predicted
1320-1343
XP_002457719
hypothetical protein
1.00
381
578


folded


SORBIDRAFT_03g012350


24-nts-


[Sorghum bicolor]


long


> gi|241929694|gb|EES02839.1|


seq


hypothetical protein


51469


SORBIDRAFT_03g012350





[Sorghum bicolor]




NP_001183022
hypothetical protein
0.85
382
579





LOC100501344 [Zea mays]





> gi|238008824|gb|ACR35447.1|





unknown [Zea mays]




XP_002457721
hypothetical protein
0.77
383
580





SORBIDRAFT_03g012360





[Sorghum bicolor]





> gi|241929696|gb|EES02841.1|





hypothetical protein





SORBIDRAFT_03g012360





[Sorghum bicolor]




XP_002457722
hypothetical protein
0.73
384
581





SORBIDRAFT_03g012370





[Sorghum bicolor]





> gi|241929697|gb|EES02842.1|





hypothetical protein





SORBIDRAFT_03g012370





[Sorghum bicolor]


zma-
158-179
NP_001147885
LOC100281495 [Zea mays]
1.00
385
582


miR399f*


> gi|195611982|gb|ACG27821.1|





citrate transporter





family protein [Zea mays]





> gi|195614372|gb|ACG29016.1|





citrate transporter





family protein [Zea mays]




ACF86945
unknown [Zea mays]
0.93
386
583




ACG28034
citrate transporter family
0.98
387
584





protein [Zea mays]




NP_001048962
Os03g0147400 [Oryza sativa
0.80
388
585





Japonica Group]





> gi|15451603|gb|AAK98727.1|





AC090485_6 Putative





anion transporter [Oryza





sativa Japonica Group]





> gi|108706182|gb|ABF93977.1|





transmembrane





protein, putative, expressed





[Oryza sativa Japonica





Group]





> gi|108706183|gb|ABF93978.1|





transmembrane





protein, putative, expressed





[Oryza sativa Japonica





Group]





> gi|108706184|gb|ABF93979.1|





transmembrane





protein, putative, expressed





[Oryza sativa Japonica





Group]





> gi|108706185|gb|ABF93980.1|





transmembrane





protein, putative, expressed





[Oryza sativa Japonica





Group]





> gi|13547433|dbj|BAF10876.1|





Os03g0147400 [Oryza





sativa Japonica Group]





> gi|125542408|gb|EAY88547.1|





hypothetical protein





OsI_10021 [Oryza sativa





Indica Group]





> gi|125584918|gb|EAZ25582.1|





hypothetical protein





OsJ_09409 [Oryza sativa





Japonica Group]




EAY78724
hypothetical protein
0.83
389





OsI_33828 [Oryza sativa





Indica Group]





> gi|125574971|gb|EAZ16255.1|





hypothetical protein





OsJ_31712 [Oryza sativa





Japonica Group]




NP_001151517
LOC100285151 [Zea mays]
0.80
390
586





> gi|195647360|gb|ACG43148.1|





citrate transporter





family protein [Zea mays]





> gi|223948219|gb|ACN28193.1|





unknown [Zea mays]





> gi|223974939|gb|ACN31657.1|





unknown [Zea mays]





> gi|238009364|gb|ACR35717.1|





unknown [Zea mays]




ACG43196
citrate transporter family
0.80
391
587





protein [Zea mays]




ACL54556
unknown [Zea mays]
0.77
392
588




BAK05230
predicted protein [Hordeum
0.78
393
589





vulgare subsp. vulgare]




BAK06146
predicted protein [Hordeum
0.78
394
590





vulgare subsp. vulgare]



650-671
XP_002460963
hypothetical protein
1.00
395
591





SORBIDRAFT_02g038300





[Sorghum bicolor]





> gi|241924340|gb|EER97484.1|





hypothetical protein





SORBIDRAFT_02g038300





[Sorghum bicolor]




NP_001148467
LOC100282082 [Zea mays]
0.92
396
592





> gi|195619570|gb|ACG31615.1|





saccharopine





dehydrogenase [Zea mays]




EAZ04584
hypothetical protein
0.79
397





OsI_26734 [Oryza sativa





Indica Group]




BAJ97022
predicted protein [Hordeum
0.79
398
593





vulgare subsp. vulgare]




EAZ40532
hypothetical protein
0.73
399





OsJ_24988 [Oryza sativa





Japonica Group]


Predicted
2327-2350
NP_001169325
hypothetical protein
1.00
400
594


folded


LOC100383191 [Zea mays]


24-nts-


> gi|224028683|gb|ACN33417.1|


long


unknown [Zea mays]


seq


52499




XP_002447125
hypothetical protein
0.98
401
595





SORBIDRAFT_06g029090





[Sorghum bicolor]





> gi|241938308|gb|EES11453.1|





hypothetical protein





SORBIDRAFT_06g029090





[Sorghum bicolor]


Predicted
287-306
XP_002447878
hypothetical protein
1.00
402
596


zma


SORBIDRAFT_06g017360


mir


[Sorghum bicolor]


50545


> gi|241939061|gb|EES12206.1|





hypothetical protein





SORBIDRAFT_06g017360





[Sorghum bicolor]




NP_001151469
translocon Tic40 [Zea mays]
0.90
403
597





> gi|219887501|gb|ACL54125.1|





unknown [Zea mays]




ACG42966
translocon Tic40 [Zea mays]
0.90
404
598




NP_001149949
translocon Tic40 [Zea mays]
0.90
405
599





> gi|195635683|gb|ACG37310.1|





translocon Tic40 [Zea





mays]




NP_001052871
Os04g0439900 [Oryza sativa
0.81
406
600





Japonica Group]





> gi|113564442|dbj|BAF14785.1|





Os04g0439900 [Oryza





sativa Japonica Group]





> gi|116309806|emb|CAH66845.1|





H0525C06.8 [Oryza





sativa Indica Group]





> gi|215704350|dbj|BAG93784.1|





unnamed protein





product [Oryza sativa





Japonica Group]





> gi|218194904|gb|EEC77331.1|





hypothetical protein





OsI_16005 [Oryza sativa





Indica Group]





> gi|222628923|gb|EEE61055.1|





hypothetical protein





OsJ_14912 [Oryza sativa





Japonica Group]




BAJ97578
predicted protein [Hordeum
0.78
407
601





vulgare subsp. vulgare]



46-65
NP_001182915
hypothetical protein
1.00
408
602





LOC100501201 [Zea mays]





> gi|238008164|gb|ACR35117.1|





unknown [Zea mays]




ACN33347
unknown [Zea mays]
0.84
409
603




NP_001150079
CID11 [Zea mays]
0.83
410
604





> gi|195636508|gb|ACG37722.1|





CID11 [Zea mays]




XP_002444926
hypothetical protein
0.78
411
605





SORBIDRAFT_07g001560





[Sorghum bicolor]





> gi|241941276|gb|EES14421.1|





hypothetical protein





SORBIDRAFT_07g001560





[Sorghum bicolor]




BAD33089
putative RNA-binding protein
0.74
412





RBP37 [Oryza sativa





Japonica Group]





> gi|182375457|dbj|BAG24017.1|





RNA-binding protein





[Oryza sativa Japonica





Group]





> gi|215736921|dbj|BAG95850.1|





unnamed protein





product [Oryza sativa





Japonica Group]





> gi|222639804|gb|EEE67936.1|





hypothetical protein





OsJ_25822 [Oryza sativa





Japonica Group]




EEC82815
hypothetical protein
0.72
413





OsI_27601 [Oryza sativa





Indica Group]




BAJ96189
predicted protein [Hordeum
0.75
414
606





vulgare subsp. vulgare]




XP_002518926
RNA-binding protein,
0.75
415
607





putative [Ricinus communis]





> gi|223541913|gb|EEF43459.1|





RNA-binding protein,





putative [Ricinus communis]




XP_002272223
PREDICTED: hypothetical
0.75
416
608





protein [Vitis vinifera]





> gi|302143972|emb|CBI23077.3|





unnamed protein





product [Vitis vinifera]



605-624
NP_001142867
hypothetical protein
1.00
417
609





LOC100275266 [Zea mays]





> gi|195610696|gb|ACG27178.1|





hypothetical protein





[Zea mays]




XP_002448750
hypothetical protein
0.83
418
610





SORBIDRAFT_06g032540





[Sorghum bicolor]





> gi|241939933|gb|EES13078.1|





hypothetical protein





SORBIDRAFT_06g032540





[Sorghum bicolor]




NP_001143447
hypothetical protein
0.78
419
611





LOC100276102 [Zea mays]





> gi|195620654|gb|ACG32157.1|





hypothetical protein





[Zea mays]




NP_001054225
Os04g0672300 [Oryza sativa
0.72
420
612





Japonica Group]





> gi|90265231|emb|CAH67766.1|





H0322F07.3 [Oryza





sativa Indica Group]





> gi|113565796|dbj|BAF16139.1|





Os04g0672300 [Oryza





sativa Japonica Group]





> gi|215715224|dbj|BAG94975.1|





unnamed protein





product [Oryza sativa





Japonica Group]



360-379
NP_001043014
Os01g0358300 [Oryza sativa
1.00
421
613





Japonica Group]





> gi|53791615|dbj|BAD52962.1|





unknown protein [Oryza





sativa Japonica Group]





> gi|113532545|dbj|BAF04928.1|





Os01g0358300 [Oryza





sativa Japonica Group]





> gi|215765732|dbj|BAG87429.1|





unnamed protein





product [Oryza sativa





Japonica Group]





> gi|218188197|gb|EEC70624.1|





hypothetical protein





OsI_01883 [Oryza sativa





Indica Group]





> gi|222618419|gb|EEE54551.1|





hypothetical protein





OsJ_01736 [Oryza sativa





Japonica Group]




ACF87799
unknown [Zea mays]
0.86
422
614




ACN30638
unknown [Zea mays]
0.85
423
615




BAJ94646
predicted protein [Hordeum
0.84
424
616





vulgare subsp. vulgare]



557-576
XP_002455496
hypothetical protein
1.00
425
617





SORBIDRAFT_03g011990





[Sorghum bicolor]





> gi|241927471|gb|EES00616.1|





hypothetical protein





SORBIDRAFT_03g011990





[Sorghum bicolor]




XP_002457709
hypothetical protein
0.98
426
618





SORBIDRAFT_03g011980





[Sorghum bicolor]





> gi|241929684|gb|EES02829.1|





hypothetical protein





SORBIDRAFT_03g011980





[Sorghum bicolor]




ACN26409
unknown [Zea mays]
0.93
427
619




ACN27987
unknown [Zea mays]
0.93
428
620




NP_001152255
peroxidase 1 [Zea mays]
0.93
429
621





> gi|195654333|gb|ACG46634.1|





peroxidase 1 precursor





[Zea mays]




EAY73614
hypothetical protein
0.81
430





OsI_01501 [Oryza sativa





Indica Group]




NP_001042800
Os01g0294700 [Oryza sativa
0.81
431
622





Japonica Group]





> gi|9909174|dbj|BAB12033.1|





putative peroxidase





[Oryza sativa Japonica





Group]





> gi|55700889|tpe|CAH69254.1|





TPA: class III





peroxidase 11 precursor





[Oryza sativa Japonica





Group]





> gi|113532331|dbj|BAF04714.1|





Os01g0294700 [Oryza





sativa Japonica Group]





> gi|215697074|dbj|BAG91068.1|





unnamed protein





product [Oryza sativa





Japonica Group]





> gi|215737508|dbj|BAG96638.1|





unnamed protein





product [Oryza sativa





Japonica Group]





> gi|215740794|dbj|BAG96950.1|





unnamed protein





product [Oryza sativa





Japonica Group]




EEE54389
hypothetical protein
0.76
432





OsJ_01407 [Oryza sativa





Japonica Group]



517-536
ACN27987
unknown [Zea mays]
1.00
433
623




NP_001152255
peroxidase 1 [Zea mays]
1.00
434
624





> gi|195654333|gb|ACG46634.1|





peroxidase 1 precursor





[Zea mays]




ACN26409
unknown [Zea mays]
0.94
435
625




XP_002457709
hypothetical protein
0.93
436
626





SORBIDRAFT_03g011980





[Sorghum bicolor]





> gi|241929684|gb|EES02829.1|





hypothetical protein





SORBIDRAFT_03g011980





[Sorghum bicolor]




XP_002455496
hypothetical protein
0.93
437
627





SORBIDRAFT_03g011990





[Sorghum bicolor]





> gi|241927471|gb|EES00616.1|





hypothetical protein





SORBIDRAFT_03g011990





[Sorghum bicolor]




EAY73614
hypothetical protein
0.81
438





OsI_01501 [Oryza sativa





Indica Group]




NP_001042800
Os01g0294700 [Oryza sativa
0.81
439
628





Japonica Group]





> gi|9909174|dbj|BAB12033.1|





putative peroxidase





[Oryza sativa Japonica





Group]





> gi|55700889|tpe|CAH69254.1|





TPA: class III





peroxidase 11 precursor





[Oryza sativa Japonica





Group]





> gi|113532331|dbj|BAF04714.1|





Os01g0294700 [Oryza





sativa Japonica Group]





> gi|215697074|dbj|BAG91068.1|





unnamed protein





product [Oryza sativa





Japonica Group]





> gi|215737508|dbj|BAG96638.1|





unnamed protein





product [Oryza sativa





Japonica Group]





> gi|215740794|dbj|BAG96950.1|





unnamed protein





product [Oryza sativa





Japonica Group]




EEE54389
hypothetical protein
0.76
440





OsJ_01407 [Oryza sativa





Japonica Group]



286-305
ACG31680
pepsin A [Zea mays]
1.00
441
629




NP_001141522
hypothetical protein
0.99
442
630





LOC100273634 [Zea mays]





> gi|194704920|gb|ACF86544.1|





unknown [Zea mays]





> gi|223949445|gb|ACN28806.1|





unknown [Zea mays]




ACL54367
unknown [Zea mays]
0.99
443
631




NP_001132197
hypothetical protein
0.89
444
632





LOC100193625 [Zea mays]





> gi|194693730|gb|ACF80949.1|





unknown [Zea mays]





> gi|195605492|gb|ACG24576.1|





pepsin A [Zea mays]




NP_001048134
Os02g0751100 [Oryza sativa
0.80
445
633





Japonica Group]





> gi|46390211|dbj|BAD15642.1|





aspartyl protease-like





[Oryza sativa Japonica





Group]





> gi|113537665|dbj|BAF10048.1|





Os02g0751100 [Oryza





sativa Japonica Group]





> gi|222623681|gb|EEE57813.1|





hypothetical protein





OsJ_08401 [Oryza sativa





Japonica Group]




EEC74016
hypothetical protein
0.79
446





OsI_08957 [Oryza sativa





Indica Group]




ACN27411
unknown [Zea mays]
0.77
447
634




BAK05106
predicted protein [Hordeum
0.75
448
635





vulgare subsp. vulgare]



309-328
NP_001143133
hypothetical protein
1.00
449
636





LOC100275611 [Zea mays]





> gi|195614826|gb|ACG29243.1|





hypothetical protein





[Zea mays]




ACF83002
unknown [Zea mays]
0.99
450
637




BAJ87435
predicted protein [Hordeum
0.72
451
638





vulgare subsp. vulgare]



46-65
XP_002444926
hypothetical protein
1.00
452
639





SORBIDRAFT_07g001560





[Sorghum bicolor]





> gi|241941276|gb|EES14421.1|





hypothetical protein





SORBIDRAFT_07g001560





[Sorghum bicolor]




ACN33347
unknown [Zea mays]
0.85
453
640




NP_001182915
hypothetical protein
0.87
454
641





LOC100501201 [Zea mays]





> gi|238008164|gb|ACR35117.1|





unknown [Zea mays]




NP_001150079
CID11 [Zea mays]
0.86
455
642





> gi|195636508|gb|ACG37722.1|





CID11 [Zea mays]




BAD33089
putative RNA-binding protein
0.75
456





RBP37 [Oryza sativa





Japonica Group]





> gi|182375457|dbj|BAG24017.1|





RNA-binding protein





[Oryza sativa Japonica





Group]





> gi|215736921|dbj|BAG95850.1|





unnamed protein





product [Oryza sativa





Japonica Group]





> gi|222639804|gb|EEE67936.1|





hypothetical protein





OsJ_25822 [Oryza sativa





Japonica Group]




EEC82815
hypothetical protein
0.72
457





OsI_27601 [Oryza sativa





Indica Group]



40563
NP_001059821
Os07g0524100 [Oryza sativa
1.00
458
643





Japonica Group]





> gi|75118816|sp|Q69SA9.1|





PDI54_ORYSJ RecName:





Full = Protein disulfide





isomerase-like 5-4;





Short = OsPDIL5-4; AltName:





Full = Protein disulfide





isomerase-like 8-1;





Short = OsPDIL8-1; Flags:





Precursor





> gi|50508559|dbj|BAD30858.1|





thioredoxin family-like





protein [Oryza sativa





Japonica Group]





> gi|113611357|dbj|BAF21735.1|





Os07g0524100 [Oryza





sativa Japonica Group]





> gi|215704615|dbj|BAG94243.1|





unnamed protein





product [Oryza sativa





Japonica Group]





> gi|218199742|gb|EEC82169.1|





hypothetical protein





OsI_26259 [Oryza sativa





Indica Group]





> gi|222637167|gb|EEE67299.1|





hypothetical protein





OsJ_24505 [Oryza sativa





Japonica Group]




BAJ86285
predicted protein [Hordeum
0.93
459
644





vulgare subsp. vulgare]




CBG91903
putative PDI-like protein
0.93
460





[Triticum aestivum]





> gi|299469398|emb|CBG91917.1|





putative PDI-like





protein [Triticum aestivum]




ACG39185
PDIL5-4-Zea mays protein
0.91
461
645





disulfide isomerase




NP_001105762
protein disulfide isomerase12
0.91
462
646





[Zea mays]





> gi|59861281|gb|AAX09970.1|





protein disulfide





isomerase [Zea mays]




ACN34146
unknown [Zea mays]
0.91
463
647




XP_002522864
thioredoxin domain-
0.76
464
648





containing protein, putative





[Ricinus communis]





> gi|223537948|gb|EEF39562.1|





thioredoxin domain-





containing protein, putative





[Ricinus communis]




XP_002281649
PREDICTED: hypothetical
0.74
465
649





protein [Vitis vinifera]





> gi|297735969|emb|CBI23943.3|





unnamed protein





product [Vitis vinifera]




XP_002319814
predicted protein [Populus
0.74
466
650





trichocarpa]





> gi|222858190|gb|EEE95737.1|





predicted protein





[Populus trichocarpa]




XP_002317580
predicted protein [Populus
0.74
467
651





trichocarpa]





> gi|222860645|gb|EEE98192.1|





predicted protein





[Populus trichocarpa]



301-320
ACF87799
unknown [Zea mays]
1.00
468
652




ACN30638
unknown [Zea mays]
0.97
469
653




NP_001043014
Os01g0358300 [Oryza sativa
0.87
470
654





Japonica Group]





> gi|53791615|dbj|BAD52962.1|





unknown protein [Oryza





sativa Japonica Group]





> gi|113532545|dbj|BAF04928.1|





Os01g0358300 [Oryza





sativa Japonica Group]





> gi|215765732|dbj|BAG87429.1|





unnamed protein





product [Oryza sativa





Japonica Group]





> gi|218188197|gb|EEC70624.1|





hypothetical protein





OsI_01883 [Oryza sativa





Indica Group]





> gi|222618419|gb|EEE54551.1|





hypothetical protein





OsJ_01736 [Oryza sativa





Japonica Group]




BAJ94646
predicted protein [Hordeum
0.83
471
655





vulgare subsp. vulgare]


Predicted
40752
ACF86440
unknown [Zea mays]
1.00
472
656


zma


mir


48350



22-43
ABF67946
putative Opie4 pol protein
1.00
473





[Zea mays]




AAC49502
Pol [Zea mays]
0.97
474
657




ABF67947
Opie2 pol protein [Zea mays]
0.96
475




ABF67934
Opie3 pol polyprotein [Zea
0.96
476





mays]




AAL35396
Opie2a pol [Zea mays]
0.92
477





> gi|168251075|gb|ACA21858.1|





Opie2a pol protein





[Zea mays]




ABF67921
Ji1 putative pol protein [Zea
0.79
478





mays]




AAD20307
copia-type pol polyprotein
0.79
479





[Zea mays]


Predicted
810-831
XP_002455047
hypothetical protein
1.00
480
658


zma


SORBIDRAFT_03g003530


mir


[Sorghum bicolor]


49162


> gi|241927022|gb|EES00167.1|





hypothetical protein





SORBIDRAFT_03g003530





[Sorghum bicolor]




AAK51797
small heat shock protein
0.83
481
659





HSP17.8 [Triticum aestivum]




BAJ86365
predicted protein [Hordeum
0.82
482
660





vulgare subsp. vulgare]




NP_001105954
17.8 kDa class II heat shock
0.87
483
661





protein [Zea mays]





> gi|123553|sp|P24632.1|HSP22_MAIZE





RecName:





Full = 17.8 kDa class II heat





shock protein





> gi|22337|emb|CAA38012.1|





18 kDa heat shock protein





[Zea mays]





> gi|238009918|gb|ACR35994.1|





unknown [Zea mays]





> gi|238015198|gb|ACR38634.1|





unknown [Zea mays]




CAI96500
17.6 kDa heat-shock protein
0.83
484
662





[Triticum turgidum subsp.





dicoccon]




BAK07165
predicted protein [Hordeum
0.81
485
663





vulgare subsp. vulgare]




CAI96499
17.5 kDa heat-shock protein
0.81
486
664





[Triticum turgidum subsp.





dicoccoides]




CAI96501
17.6 kDa heat-shock protein
0.82
487
665





[Triticum turgidum subsp.





durum]




NP_001148454
17.5 kDa class II heat shock
0.87
488
666





protein [Zea mays]





> gi|195619384|gb|ACG31522.1|





17.5 kDa class II heat





shock protein [Zea mays]




BAK05681
predicted protein [Hordeum
0.80
489
667





vulgare subsp. vulgare]


Predicted
597-618
XP_002456299
hypothetical protein
1.00
490
668


zma


SORBIDRAFT_03g033710


mir


[Sorghum bicolor]


48877


> gi|241928274|gb|EES01419.1|





hypothetical protein





SORBIDRAFT_03g033710





[Sorghum bicolor]




ACF79056
unknown [Zea mays]
0.92
491
669





> gi|194693496|gb|ACF80832.1|





unknown [Zea mays]





> gi|195626284|gb|ACG34972.1|





GTP-binding protein





[Zea mays]





> gi|223946703|gb|ACN27435.1|





unknown [Zea mays]




NP_001140242
hypothetical protein
0.92
492
670





LOC100272283 [Zea mays]





> gi|194698666|gb|ACF8417.1|





unknown [Zea mays]




NP_001044154
Os01g0732200 [Oryza sativa
0.84
493
671





Japonica Group]





> gi|57899709|dbj|BAD87429.1|





putative GTP-binding





protein [Oryza sativa





Japonica Group]





> gi|113533685|dbj|BAF06068.1|





Os01g0732200 [Oryza





sativa Japonica Group]





> gi|215695283|dbj|BAG90474.1|





unnamed protein





product [Oryza sativa





Japonica Group]




EEC71435
hypothetical protein
0.84
494





OsI_03633 [Oryza sativa





Indica Group]





> gi|222619209|gb|EEE5541.1|





hypothetical protein





OsJ_03357 [Oryza sativa





Japonica Group]



218-239
XP_002463058
hypothetical protein
1.00
495
672





SORBIDRAFT_02g036900





[Sorghum bicolor]





> gi|241926435|gb|EER99579.1|





hypothetical protein





SORBIDRAFT_02g036900





[Sorghum bicolor]




NP_001142066
hypothetical protein
0.90
496
673





LOC100274223 [Zea mays]





> gi|194706978|gb|ACF87573.1|





unknown [Zea mays]




BAJ95349
predicted protein [Hordeum
0.75
497
674





vulgare subsp. vulgare]





> gi|326526013|dbj|BAJ93183.1|





predicted protein





[Hordeum vulgare subsp.





vulgare]




EAZ40364
hypothetical protein
0.73
498





OsJ_24810 [Oryza sativa





Japonica Group]




EAZ04406
hypothetical protein
0.73
499





OsI_26550 [Oryza sativa





Indica Group]


Predicted
63-86
AAD11615
prpol [Zea mays]
1.00
500
675


folded


24-nts-


long


seq


52057




AAL76007
prpol [Zea mays]
0.97
501



907-930
AAD11616
prpol [Zea mays]
1.00
502
676


Predicted
387-410
XP_002445682
hypothetical protein
1.00
503
677


folded


SORBIDRAFT_07g024170


24-nts-


[Sorghum bicolor]


long


> gi|241942032|gb|EES15177.1|


seq


hypothetical protein


52633


SORBIDRAFT_07g024170





[Sorghum bicolor]




ACF83553
unknown [Zea mays]
0.90
504
678





> gi|195646380|gb|ACG42658.1|





glutathione S-





transferase, N-terminal





domain containing protein





[Zea mays]




ACN26529
unknown [Zea mays]
0.89
505
679


Predicted
450-470
NP_001141971
hypothetical protein
1.00
506
680


zma


LOC100274121 [Zea mays]


mir


> gi|194706630|gb|ACF87399.1|


48922


unknown [Zea mays]




XP_002455881
hypothetical protein
0.79
507
681





SORBIDRAFT_03g026750





[Sorghum bicolor]





> gi|241927856|gb|EES01001.1|





hypothetical protein





SORBIDRAFT_03g026750





[Sorghum bicolor]


Predicted
1046-1067
XP_002465053
hypothetical protein
1.00
508
682


zma


SORBIDRAFT_01g031330


mir


[Sorghum bicolor]


50077


> gi|241918907|gb|EER92051.1|





hypothetical protein





SORBIDRAFT_01g031330





[Sorghum bicolor]




NP_001137129
hypothetical protein
0.90
509
683





LOC100217311 [Zea mays]





> gi|194698486|gb|ACF83327.1|





unknown [Zea mays]




NP_001149312
fas-associated factor 1-like
0.93
510
684





protein [Zea mays]





> gi|195626306|gb|ACG34983.1|





fas-associated factor 1-





like protein [Zea mays]





> gi|223975789|gb|ACN32082.1|





unknown [Zea mays]




BAJ89712
predicted protein [Hordeum
0.83
511
685





vulgare subsp. vulgare]





> gi|326495414|dbj|BAJ85803.1|





predicted protein





[Hordeum vulgare subsp.





vulgare]




AAG13433
unknown protein [Oryza
0.85
512
686





sativa Japonica Group]





> gi|31433109|gb|AAP54662.1|





UBX domain containing





protein, expressed [Oryza





sativa Japonica Group]





> gi|215678662|dbj|BAG92317.1|





unnamed protein





product [Oryza sativa





Japonica Group]




EAY79232
hypothetical protein
0.85
513





OsI_34349 [Oryza sativa





Indica Group]




EAZ16707
hypothetical protein
0.81
514





OsJ_32183 [Oryza sativa





Japonica Group]





Table 5: Provided are the target Genes of miRNAs Associated with Increased NUE (Table 1) along with their GenBank Accession numbers and sequence identifiers (SEQ ID NO:).


“bind” = binding;


“pos” = position;


“hom” = homologue;


“p.p.” = polypeptide;


“p.n.” = polynucleotide.













TABLE 6







Target Genes of down regulated miRNAs Associated with Increased NUE


(Table 2)














Mir
Hom.


p.p.
p.n.


Mir
Bind.
NCBI


SEQ ID
SEQ


name
Pos.
Accession
Annotation/Organism
Identity
NO:
ID NO:
















Predicted
743-763
NP_001148146
terpene synthase 7 [Zea mays]
1.00
687
1392


zma


> gi|195616112|gb|ACG29886.1|


mir


terpene synthase 7 [Zea


50318


mays]




ACL54589
unknown [Zea mays]
0.99
688
1393




XP_002447434
hypothetical protein
0.71
689
1394





SORBIDRAFT_06g001020





[Sorghum bicolor]





> gi|241938617|gb|EES11762.1|





hypothetical protein





SORBIDRAFT_06g001020





[Sorghum bicolor]



776-796
XP_002455470
hypothetical protein
1.00
690
1395





SORBIDRAFT_03g011420





[Sorghum bicolor]





> gi|241927445|gb|EES00590.1|





hypothetical protein





SORBIDRAFT_03g011420





[Sorghum bicolor]




NP_001151624
cyclin-like F-box [Zea mays]
0.90
691
1396





> gi|195648178|gb|ACG43557.1|





cyclin-like F-box [Zea





mays]




BAJ88045
predicted protein [Hordeum
0.79
692
1397





vulgare subsp. vulgare]





> gi|326521874|dbj|BAK04065.1|





predicted protein





[Hordeum vulgare subsp.





vulgare]




NP_001042756
Os01g0281000 [Oryza sativa
0.81
693
1398





Japonica Group]





> gi|6498442|dbj|BAA87845.1|





unknown protein [Oryza





sativa Japonica Group]





> gi|11041564|dbj|BAB00648.2|





unnamed protein product





[Oryza sativa Japonica Group]





> gi|11138071|dbj|BAB17744.1|





OSJNBa0036E02.18





[Oryza sativa Japonica Group]





> gi|13873014|dbj|BAB44118.1|





unknown protein [Oryza





sativa Japonica Group]





> gi|113532287|dbj|BAF04670.1|





Os01g0281000 [Oryza





sativa Japonica Group]





> gi|215694300|dbj|BAG89293.1|





unnamed protein





product [Oryza sativa Japonica





Group]




EEE54342
hypothetical protein
0.75
694





OsJ_01321 [Oryza sativa





Japonica Group]




EEC70414
hypothetical protein
0.75
695





OsI_01411 [Oryza sativa





Indica Group]



2058-2078
XP_002468633
hypothetical protein
1.00
696
1399





SORBIDRAFT_01g049370





[Sorghum bicolor]





> gi|241922487|gb|EER95631.1|





hypothetical protein





SORBIDRAFT_01g049370





[Sorghum bicolor]




NP_001130361
hypothetical protein
0.98
697
1400





LOC100191456 [Zea mays]





> gi|194688936|gb|ACF78552.1|





unknown [Zea mays]





> gi|194707734|gb|ACF87951.1|





unknown [Zea mays]




NP_001167830
hypothetical protein
0.96
698
1401





LOC100381530 [Zea mays]





> gi|223944309|gb|ACN26238.1|





unknown [Zea mays]




NP_001046725
Os02g0332200 [Oryza sativa
0.95
699
1402





Japonica Group]





> gi|46390985|dbj|BAD16520.1|





putative cytosolic





chaperonin delta-subunit





[Oryza sativa Japonica Group]





> gi|113536256|dbj|BAF08639.1|





Os02g0332200 [Oryza





sativa Japonica Group]





> gi|215695341|dbj|BAG90532.1|





unnamed protein





product [Oryza sativa Japonica





Group]





> gi|222636848|gb|EEE66980.1|





hypothetical protein





OsJ_23870 [Oryza sativa





Japonica Group]




EAZ06856
hypothetical protein
0.95
700





OsI_29091 [Oryza sativa





Indica Group]




BAJ97289
predicted protein [Hordeum
0.92
701
1403





vulgare subsp. vulgare]




NP_001065054
Os10g0514600 [Oryza sativa
0.88
702
1404





Japonica Group]





> gi|10140686|gb|AAG13521.1|





AC068924_26 putative





cytosolic chaperonin, delta-





subunit [Oryza sativa Japonica





Group]





> gi|31433047|gb|AAP54607.1|





T-complex protein 1, delta





subunit, putative, expressed





[Oryza sativa Japonica Group]





> gi|113639663|dbj|BAF26968.1|





Os10g0514600 [Oryza





sativa Japonica Group]





> gi|215687328|dbj|BAG91872.1|





unnamed protein





product [Oryza sativa Japonica





Group]




XP_002322076
predicted protein [Populus
0.81
703
1405





trichocarpa]





> gi|222869072|gb|EEF06203.1|





predicted protein [Populus





trichocarpa]




XP_002317895
predicted protein [Populus
0.80
704
1406





trichocarpa]





> gi|222858568|gb|EEE96115.1|





predicted protein





[Populus trichocarpa]




CAN70636
hypothetical protein
0.82
705
1407





VITISV_008621 [Vitis





vinifera]



554-574
XP_002465983
hypothetical protein
1.00
706
1408





SORBIDRAFT_01g049510





[Sorghum bicolor]





> gi|241919837|gb|EER92981.1|





hypothetical protein





SORBIDRAFT_01g049510





[Sorghum bicolor]




NP_001149716
fusca homolog [Zea mays]
0.95
707
1409





> gi|195629716|gb|ACG36499.1|





COP9 signalosome





complex subunit 1 [Zea mays]




NP_00148760
Os03g0116500 [Oryza sativa
0.89
708
1410





Japonica Group]





> gi|108705858|gb|ABF93653.1|





COP9 signalosome





complex subunit 1, putative,





expressed [Oryza sativa





Japonica Group]





> gi|113547231|dbj|BAF10674.1|





Os03g0116500 [Oryza





sativa Japonica Group]





> gi|125542143|gb|EAY88282.1|





hypothetical protein





OsI_09737 [Oryza sativa





Indica Group]





> gi|125584695|gb|EAZ25359.1|





hypothetical protein





OsJ_09174 [Oryza sativa





Japonica Group]





> gi|215678827|dbj|BAG95264.1|





unnamed protein





product [Oryza sativa Japonica





Group]




AAF40112
constitutive photomorphogenic
0.88
709
1411





11 [Oryza sativa Indica Group]




AAG17476
rCOP11 protein [Oryza sativa
0.88
710
1412





Indica Group]




BAJ99160
predicted protein [Hordeum
0.84
711
1413





vulgare subsp. vulgare]




BAK01545
predicted protein [Hordeum
0.78
712
1414





vulgare subsp. vulgare]




XP_002275895
PREDICTED: hypothetical
0.72
713
1415





protein [Vitis vinifera]





> gi|296089065|emb|CBI38768.3|





unnamed protein





product [Vitis vinifera]




CAN74681
hypothetical protein
0.72
714





VITISV_025856 [Vitis





vinifera]



372-392
XP_002448372
hypothetical protein
1.00
715
1416





SORBIDRAFT_06g026160





[Sorghum bicolor]





> gi|241939555|gb|EES12700.1|





hypothetical protein





SORBIDRAFT_06g026160





[Sorghum bicolor]




ADZ96243
ACC synthase 1 [Saccharum
0.92
716
1417





hybrid cultivar SP80-3280]




AAR25558
acc synthase [Zea mays]
0.92
717
1418




AAR25559
acc synthase [Zea mays]
0.91
718
1419




NP_001146401
1-aminocyclopropane-1-
0.91
719
1420





carboxylate synthase7 [Zea





mays]





> gi|219886697|gb|ACL53723.1|





unknown [Zea mays]




ACN31344
unknown [Zea mays]
0.90
720
1421




BAJ95898
predicted protein [Hordeum
0.81
721
1422





vulgare subsp. vulgare]




NP_001053637
Os04g0578000 [Oryza sativa
0.80
722
1423





Japonica Group]





> gi|32488497|emb|CAE03249.1|





OSJNBa0011J08.4





[Oryza sativa Japonica Group]





> gi|113565208|dbj|BAF15551.1|





Os04g0578000 [Oryza





sativa Japonica Group]





> gi|215693849|dbj|BAG89048.1|





unnamed protein





product [Oryza sativa Japonica





Group]




CAH66732
H0404F02.8 [Oryza sativa
0.80
723
1424





Indica Group]





> gi|125549433|gb|EAY95255.1|





hypothetical protein





OsI_17075 [Oryza sativa





Indica Group]




AAB18416
ACC synthase [Triticum
0.80
724
1425





aestivum]



1204-1224
ACN26771
unknown [Zea mays]
1.00
725
1426




ACG31388
F-box domain containing
1.00
726
1427





protein [Zea mays]




ACG45892
F-box domain containing
1.00
727
1428





protein [Zea mays]




ACG25717
F-box domain containing
0.99
728
1429





protein [Zea mays]




NP_001152119
F-box domain containing
0.96
729
1430





protein [Zea mays]





> gi|195652827|gb|ACG45881.1|





F-box domain containing





protein [Zea mays]




XP_002467684
hypothetical protein
0.89
730
1431





SORBIDRAFT_01g032310





[Sorghum bicolor]





> gi|241921538|gb|EER94682.1|





hypothetical protein





SORBIDRAFT_01g032310





[Sorghum bicolor]




EAY90508
hypothetical protein
0.82
731





OsI_12108 [Oryza sativa





Indica Group]




NP_001050391
Os03g0423000 [Oryza sativa
0.82
732
1432





Japonica Group]





> gi|30089733|gb|AAP20837.1|





expressed protein [Oryza





sativa Japonica Group]





> gi|108708889|gb|ABF96684.1|





F-box domain containing





protein, expressed [Oryza





sativa Japonica Group]





> gi|113548862|dbj|BAF12305.1|





Os03g0423000 [Oryza





sativa Japonica Group]





> gi|125586708|gb|EAZ27372.1|





hypothetical protein





OsJ_11320 [Oryza sativa





Japonica Group]





> gi|215697584|dbj|BAG91578.1|





unnamed protein





product [Oryza sativa Japonica





Group]



1019-1039
XP_002464111
hypothetical protein
1.00
733
1433





SORBIDRAFT_01g012470





[Sorghum bicolor]





> gi|241917965|gb|EER91109.1|





hypothetical protein





SORBIDRAFT_01g012470





[Sorghum bicolor]




NP_001140844
hypothetical protein
0.97
734
1434





LOC100272920 [Zea mays]





> gi|194701412|gb|ACF84790.1|





unknown [Zea mays]





> gi|223943945|gb|ACN26056.1|





unknown [Zea mays]




NP_001149791
transmembrane protein 56
0.94
735
1435





[Zea mays]





> gi|195634693|gb|ACG36815.1|





transmembrane protein





56 [Zea mays]




NP_001050848
Os03g0666700 [Oryza sativa
0.90
736
1436





Japonica Group]





> gi|40538924|gb|AAR87181.1|





expressed protein [Oryza





sativa Japonica Group]





> gi|108710279|gb|ABF98074.1|





expressed protein [Oryza





sativa Japonica Group]





> gi|113549319|dbj|BAF12762.1|





Os03g0666700 [Oryza





sativa Japonica Group]





> gi|215692429|dbj|BAG87849.1|





unnamed protein





product [Oryza sativa Japonica





Group]





> gi|215692647|dbj|BAG88067.1|





unnamed protein





product [Oryza sativa Japonica





Group]





> gi|215716974|dbj|BAG95337.1|





unnamed protein





product [Oryza sativa Japonica





Group]




BAJ94278
predicted protein [Hordeum
0.91
737
1437





vulgare subsp. vulgare]





> gi|326505624|dbj|BAJ95483.1|





predicted protein





[Hordeum vulgare subsp.





vulgare]





> gi|326513826|dbj|BAJ87931.1|





predicted protein





[Hordeum vulgare subsp.





vulgare]





> gi|326518414|dbj|BAJ88236.1|





predicted protein





[Hordeum vulgare subsp.





vulgare]




ACF84098
unknown [Zea mays]
0.93
738
1438




EEE53990
hypothetical protein
0.90
739





OsJ_00621 [Oryza sativa





Japonica Group]



1917-1937
XP_002453927
hypothetical protein
1.00
740
1439





SORBIDRAFT_04g021570





[Sorghum bicolor]





> gi|241933758|gb|EES06903.1|





hypothetical protein





SORBIDRAFT_04g021570





[Sorghum bicolor]




NP_001105849
putative glycosyltransferase
0.96
741
1440





[Zea mays]





> gi|84794314|emb|CAJ57382.1|





putative





glycosyltransferase [Zea mays]




BAK03947
predicted protein [Hordeum
0.86
742
1441





vulgare subsp. vulgare]




NP_001047014
Os02g0529600 [Oryza sativa
0.86
743
1442





Japonica Group]





> gi|49388322|dbj|BAD25434.1|





putative galactomannan





galactosyltransferase [Oryza





sativa Japonica Group]





> gi|113536545|dbj|BAF08928.1|





Os02g0529600 [Oryza





sativa Japonica Group]





> gi|215704838|dbj|BAG94866.1|





unnamed protein





product [Oryza sativa Japonica





Group]





> gi|218190881|gb|EEC73308.1|





hypothetical protein





OsI_07488 [Oryza sativa





Indica Group]





> gi|222622984|gb|EEE57116.1|





hypothetical protein





OsJ_06989 [Oryza sativa





Japonica Group]



1590-1610
NP_001131407
hypothetical protein
1.00
744
1443





LOC100192736 [Zea mays]





> gi|194691442|gb|ACF79805.1





unknown [Zea mays]




NP_001141840
hypothetical protein
0.78
745
1444





LOC100273982 [Zea mays]





> gi|194706136|gb|ACF87152.1|





unknown [Zea mays]



510-530
XP_002460574
hypothetical protein
1.00
746
1445





SORBIDRAFT_02g031070





[Sorghum bicolor]





> gi|241923951|gb|EER97095.1|





hypothetical protein





SORBIDRAFT_02g031070





[Sorghum bicolor]




NP_001151754
collagen, type IV, alpha 5 [Zea
0.87
747
1446





mays]





> gi|195649521|gb|ACG44228.1





collagen, type IV, alpha





5 [Zea mays]



876-896
XP_002437709
hypothetical protein
1.00
748
1447





SORBIDRAFT_10g001120





[Sorghum bicolor]





> gi|241915932|gb|EER89076.1|





hypothetical protein





SORBIDRAFT_10g001120





[Sorghum bicolor]




ACN34709
unknown [Zea mays]
0.99
749
1448




ACN28096
unknown [Zea mays]
0.99
750
1449





> gi|223948483|gb|ACN28325.1|





unknown [Zea mays]




NP_001056601
Os06g0114000 [Oryza sativa
0.97
751
1450





Japonica Group]





> gi|7248401|dbj|BAA92724.1|





putative chaperonin 60





beta precursor [Oryza sativa





Japonica Group]





> gi|113594641|dbj|BAF18515.1|





Os06g0114000 [Oryza





sativa Japonica Group]





> gi|164375533|gb|ABY52934.1|





RuBisCo subunit





binding-protein beta subunit





[Oryza sativa Japonica Group]





> gi|215715264|dbj|BAG95015.1|





unnamed protein





product [Oryza sativa Japonica





Group]




EEE64974
hypothetical protein
0.93
752





OsJ_19883 [Oryza sativa





Japonica Group]




NP_001045601
Os02g0102900 [Oryza sativa
0.87
753
1451





Japonica Group]





> gi|41052909|dbj|BAD07821.1|





putative RuBisCO





subunit binding-protein beta





subunit, chloroplast precursor





[Oryza sativa Japonica Group]





> gi|113535132|dbj|BAF07515.1|





Os02g0102900 [Oryza





sativa Japonica Group]





> gi|218189867|gb|EEC72294.1|





hypothetical protein





OsI_05469 [Oryza sativa





Indica Group]





> gi|222621999|gb|EEE56131.1|





hypothetical protein





OsJ_05004 [Oryza sativa





Japonica Group]





> gi|313575775|gb|ADR66967.1|





60 kDa chaperonin beta





subunit [Oryza sativa Japonica





Group]




XP_002285746
PREDICTED: hypothetical
0.88
754
1452





protein [Vitis vinifera]





> gi|297746501|emb|CBI16557.3|





unnamed protein





product [Vitis vinifera]




XP_002303983
predicted protein [Populus
0.87
755
1453





trichocarpa]





> gi|222841415|gb|EEE78962.1|





predicted protein





[Populus trichocarpa]




XP_002523404
rubisco subunit binding-
0.87
756
1454





protein beta subunit, rubb,





putative [Ricinus communis]





> gi|223537354|gb|EEF38983.1|





rubisco subunit binding-





protein beta subunit, rubb,





putative [Ricinus communis]




XP_002894506
CPN60B [Arabidopsis lyrata
0.87
757
1455





subsp. lyrata]





> gi|297340348|gb|EFH70765.1|





CPN60B [Arabidopsis





lyrata subsp. lyrata]



640-660
XP_002456142
hypothetical protein
1.00
758
1456





SORBIDRAFT_03g031180





[Sorghum bicolor]





> gi|241928117|gb|EES01262.1|





hypothetical protein





SORBIDRAFT_03g031180





[Sorghum bicolor]




NP_001148838
LOC100282456 [Zea mays]
0.94
759
1457





> gi|195622536|gb|ACG33098.1|





THAP domain-





containing protein 4 [Zea





mays]




EEC71270
hypothetical protein
0.87
760





OsI_03268 [Oryza sativa





Indica Group]




CBH32601
conserved hypothetical
0.83
761
1458





protein, expressed [Triticum





aestivum]




NP_001043867
Os01g0679600 [Oryza sativa
0.80
762
1459





Japonica Group]





> gi|56202146|dbj|BAD73479.1|





unknown protein [Oryza





sativa Japonica Group]





> gi|255673554|dbj|BAF05781.2|





Os01g0679600 [Oryza





sativa Japonica Group]




BAJ94714
predicted protein [Hordeum
0.78
763
1460





vulgare subsp. vulgare]




BAD73478
unknown protein [Oryza sativa
0.78
764
1461





Japonica Group]





> gi|215693000|dbj|BAG88420.1|





unnamed protein





product [Oryza sativa Japonica





Group]





> gi|222619045|gb|EEE55177.1|





hypothetical protein





OsJ_03010 [Oryza sativa





Japonica Group]



1204-1224
ACR38511
unknown [Zea mays]
1.00
765
1462




ACG45146
hypothetical protein [Zea
1.00
766
1463





mays]




XP_002440220
hypothetical protein
0.71
767
1464





SORBIDRAFT_09g027950





[Sorghum bicolor]





> gi|241945505|gb|EES18650.1|





hypothetical protein





SORBIDRAFT_09g027950





[Sorghum bicolor]




NP_001144829
hypothetical protein
0.71
768
1465





LOC100277913 [Zea mays]





> gi|195647628|gb|ACG43282.1|





hypothetical protein [Zea





mays]



916-936
NP_001143951
hypothetical protein
1.00
769
1466





LOC100276764 [Zea mays]





> gi|195631554|gb|ACG36672.1|





hypothetical protein [Zea





mays]




NP_001169519
hypothetical protein
0.81
770
1467





LOC100383393 [Zea mays]





> gi|224029837|gb|ACN33994.1|





unknown [Zea mays]



1178-1198
XP_002468109
hypothetical protein
1.00
771
1468





SORBIDRAFT_01g039740





[Sorghum bicolor]





> gi|241921963|gb|EER95107.1|





hypothetical protein





SORBIDRAFT_01g039740





[Sorghum bicolor]




NP_001131833
hypothetical protein
0.95
772
1469





LOC100193208 [Zea mays]





> gi|194688576|gb|ACF78372.1|





unknown [Zea mays]





> gi|195627610|gb|ACG35635.1|





protein binding protein





[Zea mays]




ACL53375
unknown [Zea mays]
0.94
773
1470




NP_001147045
protein binding protein [Zea
0.93
774
1471





mays]





> gi|195606842|gb|ACG25251.1|





protein binding protein





[Zea mays]




ABF95226
zinc finger family protein,
0.86
775





putative, expressed [Oryza





sativa Japonica Group]





> gi|108707432|gb|ABF95227.1|





zinc finger family





protein, putative, expressed





[Oryza sativa Japonica Group]





> gi|218192524|gb|EEC74951.1|





hypothetical protein





OsI_10935 [Oryza sativa





Indica Group]





> gi|222624650|gb|EEE58782.1|





hypothetical protein





OsJ_10309 [Oryza sativa





Japonica Group]




BAJ90599
predicted protein [Hordeum
0.81
776
1472





vulgare subsp. vulgare]




ADX86798
zinc-finger transcription factor
0.79
777
1473





[Triticum aestivum]




ACN35095
unknown [Zea mays]
0.70
778
1474




NP_001049696
Os03g0272300 [Oryza sativa
0.70
779
1475





Japonica Group]





> gi|108707430|gb|ABF95225.1|





zinc finger family





protein, putative, expressed





[Oryza sativa Japonica Group]





> gi|215765669|dbj|BAG87366.1|





unnamed protein





product [Oryza sativa Japonica





Group]





> gi|255674397|dbj|BAF11610.2|





Os03g0272300 [Oryza





sativa Japonica Group]



1068-1088
NP_001144727
hypothetical protein
1.00
780
1476





LOC100277769 [Zea mays]





> gi|195646260|gb|ACG42598.1|





hypothetical protein [Zea





mays]




NP_001141817
hypothetical protein
0.99
781
1477





LOC100273956 [Zea mays]





> gi|194706038|gb|ACF87103.1|





unknown [Zea mays]




NP_001170260
hypothetical protein
0.96
782
1478





LOC100384217 [Zea mays]





> gi|224034675|gb|ACN36413.1|





unknown [Zea mays]




NP_001170241
hypothetical protein
0.71
783
1479





LOC100384195 [Zea mays]





> gi|224034547|gb|ACN36349.1|





unknown [Zea mays]



573-593
XP_002454448
hypothetical protein
1.00
784
1480





SORBIDRAFT_04g031260





[Sorghum bicolor]





> gi|241934279|gb|EES07424.1|





hypothetical protein





SORBIDRAFT_04g031260





[Sorghum bicolor]




NP_001149829
LOC100283456 [Zea mays]
0.97
785
1481





> gi|195634913|gb|ACG36925.1|





acetylornithine





deacetylase [Zea mays]




EEC73818
hypothetical protein
0.85
786





OsI_08537 [Oryza sativa





Indica Group]




NP_001047794
Os02g0690800 [Oryza sativa
0.85
787
1482





Japonica Group]





> gi|41053258|dbj|BAD07684.1|





putative silverleaf





whitefly-induced protein 1





[Oryza sativa Japonica Group]





> gi|113537325|dbj|BAF09708.1|





Os02g0690800 [Oryza





sativa Japonica Group]





> gi|215715374|dbj|BAG95125.1|





unnamed protein





product [Oryza sativa Japonica





Group]





> gi|215740943|dbj|BAG97438.1|





unnamed protein





product [Oryza sativa Japonica





Group]





> gi|222623478|gb|EEE57610.1|





hypothetical protein





OsJ_07995 [Oryza sativa





Japonica Group]



1820-1840
XP_002456060
hypothetical protein
1.00
788
1483





SORBIDRAFT_03g029660





[Sorghum bicolor]





> gi|241928035|gb|EES01180.1|





hypothetical protein





SORBIDRAFT_03g029660





[Sorghum bicolor]




ACG31412
wax synthase isoform 3 [Zea
0.86
789
1484





mays]




NP_001150127
wax synthase [Zea mays]
0.86
790
1485





> gi|195636988|gb|ACG37962.1|





wax synthase isoform 3





[Zea mays]




NP_001043733
Os01g0651500 [Oryza sativa
0.73
791
1486





Japonica Group]





> gi|20146429|dbj|BAB89209.1|





wax synthase-like [Oryza





sativa Japonica Group]





> gi|113533264|dbj|BAF05647.1|





Os01g0651500 [Oryza





sativa Japonica Group]





> gi|125571401|gb|EAZ12916.1|





hypothetical protein





OsJ_02839 [Oryza sativa





Japonica Group]




EAY75194
hypothetical protein
0.72
792





OsI_03086 [Oryza sativa





Indica Group]




BAK07830
predicted protein [Hordeum
0.70
793
1487





vulgare subsp. vulgare]



1709-1729
XP_002448959
hypothetical protein
1.00
794
1488





SORBIDRAFT_05g002450





[Sorghum bicolor]





> gi|241934802|gb|EES07947.1|





hypothetical protein





SORBIDRAFT_05g002450





[Sorghum bicolor]




ACF84627
unknown [Zea mays]
0.95
795
1489





> gi|223942887|gb|ACN25527.1|





unknown [Zea mays]




NP_001150994
PP2A regulatory subunit
0.95
796
1490





TAP46 [Zea mays]





> gi|195643460|gb|ACG41198.1|





PP2A regulatory subunit





TAP46 [Zea mays]




ACF84366
unknown [Zea mays]
0.94
797
1491




EAY79895
hypothetical protein
0.89
798





OsI_35058 [Oryza sativa





Indica Group]




NP_001066109
Os12g0137500 [Oryza sativa
0.89
799
1492





Japonica Group]





> gi|77552970|gb|ABA95766.1|





TAP42-like family





protein, expressed [Oryza





sativa Japonica Group]





> gi|113648616|dbj|BAF29128.1|





Os12g0137500 [Oryza





sativa Japonica Group]





> gi|2157690835|dbj|BAH01312.1|





unnamed protein





product [Oryza sativa Japonica





Group]




EAZ19586
hypothetical protein
0.89
800





OsJ_35163 [Oryza sativa





Japonica Group]




AAO72555
PP2A regulatory subunit-like
0.89
801
1493





protein [Oryza sativa Japonica





Group]




ABA91420
TAP42-like family protein,
0.88
802
1494





expressed [Oryza sativa





Japonica Group]





> gi|222615497|gb|EEE51629.1|





hypothetical protein





OsJ_32912 [Oryza sativa





Japonica Group]




NP_001148561
PP2A regulatory subunit
0.86
803
1495





TAP46 [Zea mays]





> gi|195620440|gb|ACG32050.1|





PP2A regulatory subunit





TAP46 [Zea mays]



1417-1437
XP_002465624
hypothetical protein
1.00
804
1496





SORBIDRAFT_01g042450





[Sorghum bicolor]





> gi|194326198|emb|CAQ86599.1|





glutamine synthetase





[Sorghum bicolor]





> gi|241919478|gb|EER92622.1|





hypothetical protein





SORBIDRAFT_01g042450





[Sorghum bicolor]




AAW21274
glutamine synthetase
0.98
805
1497





[Saccharum officinarum]




NP_001105538
glutamine synthetase root
0.97
806
1498





isozyme 1 [Zea mays]





> gi|6996235|dbj|BAA03433.1|





glutamine synthetase [Zea





mays]





> gi|194701040|gb|ACF84604.1|





unknown [Zea mays]




P38559
RecName: Full = Glutamine
0.97
807





synthetase root isozyme 1;





AltName: Full = GS122;





AltName: Full = Glutamate--





ammonia ligase





> gi|434324|emb|CAA46719.1|





glutamine synthetase [Zea





mays]




P38563
RecName: Full = Glutamine
0.96
808





synthetase root isozyme 5;





AltName: Full = GS117;





AltName: Full = Glutamate--





ammonia ligase




NP_001105297
glutamine synthetase root
0.96
809
1499





isozyme 5 [Zea mays]





> gi|699621|dbj|BAA03432.1|





glutamine synthetase [Zea





mays]




ACG47508
glutamine synthetase root
0.95
810
1500





isozyme 5 [Zea mays]




NP_001049424
Os03g0223400 [Oryza sativa
0.91
811
1501





Japonica Group]





> gi|121332|sp|P14654.1|GLN12_ORYSJ





RecName:





Full = Glutamine synthetase





cytosolic isozyme 1-2;





AltName: Full = Glutamate--





ammonia ligase GLN1; 2;





Short = OsGLN1; 2; AltName:





Full = Glutamine synthetase





root isozyme; AltName:





Full = OsGS1; 2





> gi|20358|emb|CAA32460.1|





unnamed protein product





[Oryza sativa Japonica Group]





> gi|565414215|dbj|BAD77931.1|





cytosolic glutamine





synthetase 1; 2 [Oryza sativa





Japonica Group]





> gi|108706922|gb|ABF94717.1|





Glutamine synthetase





root isozyme 5, putative,





expressed [Oryza sativa





Japonica Group]





> gi|1135478955|dbj|BAF11338.1|





Os03g0223400 [Oryza





sativa Japonica Group]





> gi|215769188|dbj|BAH01417.1|





unnamed protein





product [Oryza sativa Japonica





Group]





> gi|218192361|gb|EEC74788.1|





hypothetical protein





OsI_10575 [Oryza sativa





Indica Group]





> gi|222624488|gb|EEE58620.1|





hypothetical protein





OsJ_09974 [Oryza sativa





Japonica Group]




AAR84347
glutamine synthetase isoform
0.89
812
1502





GSr1 [Triticum aestivum]




AAR84348
glutamine synthetase isoform
0.89
813
1503





GSr2 [Triticum aestivum]



1527-1547
XP_002454761
hypothetical protein
1.00
814
1504





SORBIDRAFT_04g036890





[Sorghum bicolor]





> gi|241934592|gb|EES07737.1|





hypothetical protein





SORBIDRAFT_04g036890





[Sorghum bicolor]




NP_001130621
hypothetical protein
0.87
815
1505





LOC100191720 [Zea mays]





> gi|194689664|gb|ACF78916.1|





unknown [Zea mays]




XP_002454762
hypothetical protein
0.78
816
1506





SORBIDRAFT_04g036900





[Sorghum bicolor]





> gi|241934593|gb|EES07738.1|





hypothetical protein





SORBIDRAFT_04g036900





[Sorghum bicolor]




XP_002437453
hypothetical protein
0.70
817
1507





SORBIDRAFT_10g027360





[Sorghum bicolor]





> gi|241915676|gb|EER88820.1|





hypothetical protein





SORBIDRAFT_10g027360





[Sorghum bicolor]




XP_002438878
hypothetical protein
0.69
818
1508





SORBIDRAFT_10g027650





[Sorghum bicolor]





> gi|241917101|gb|EER90245.1|





hypothetical protein





SORBIDRAFT_10g027650





[Sorghum bicolor]




XP_002438877
hypothetical protein
0.70
819
1509





SORBIDRAFT_10g027640





[Sorghum bicolor]





> gi|241917100|gb|EER90244.1|





hypothetical protein





SORBIDRAFT_10g027640





[Sorghum bicolor]



2363-2383
XP_002436826
hypothetical protein
1.00
820
1510





SORBIDRAFT_10g009530





[Sorghum bicolor]





> gi|241915049|gb|EER88193.1|





hypothetical protein





SORBIDRAFT_10g009530





[Sorghum bicolor]




ACN31818
unknown [Zea mays]
0.94
821
1511




BAJ98035
predicted protein [Hordeum
0.81
822
1512





vulgare subsp. vulgare]




NP_001057335
Os06g0264500 [Oryza sativa
0.79
823
1513





Japonica Group]





> gi|53793159|dbj|BAD54367.1|





putative nitrite





transporter [Oryza sativa





Japonica Group]





> gi|53793165|dbj|BAD54372.1|





putative nitrite





transporter [Oryza sativa





Japonica Group]





> gi|113595375|dbj|BAF19249.1|





Os06g0264500 [Oryza





sativa Japonica Group]





> gi|215767857|dbj|BAH00086.1|





unnamed protein





product [Oryza sativa Japonica





Group]





> gi|218197932|gb|EEC80359.1|





hypothetical protein





OsI_22454 [Oryza sativa





Indica Group]





> gi|222635351|gb|EEE65483.1|





hypothetical protein





OsJ_20894 [Oryza sativa





Japonica Group]



2309-2329
XP_002446671
hypothetical protein
1.00
824
1514





SORBIDRAFT_06g020190





[Sorghum bicolor]





> gi|241937854|gb|EES10999.1|





hypothetical protein





SORBIDRAFT_06g020190





[Sorghum bicolor]




NP_001151527
ATSWI3A [Zea mays]
0.92
825
1515





> gi|195647424|gb|ACG43180.1|





ATSWI3A [Zea mays]




NP_001053100
Os04g0480300 [Oryza sativa
0.76
826
1516





Japonica Group]





> gi|113564671|dbj|BAF15014.1|





Os04g0480300 [Oryza





sativa Japonica Group]





> gi|215734840|dbj|BAG95562.1|





unnamed protein





product [Oryza sativa Japonica





Group]




CAH66875
OSIGBa0158F13.6 [Oryza
0.76
827
1517





sativa Indica Group]




EEC77490
hypothetical protein
0.73
828





OsI_16334 [Oryza sativa





Indica Group]



1469-1489
NP_001148712
plant-specific domain
1.00
829
1518





TIGR01589 family protein





[Zea mays]





> gi|195621580|gb|ACG32620.1|





plant-specific domain





TIGR01589 family protein





[Zea mays]



2030-2050
NP_001105116
SBP-domain protein3 [Zea
1.00
830
1519





mays]





> gi|5931782|emb|CAB56629.1|





SBP-domain protein 3





[Zea mays]




XP_002437398
hypothetical protein
0.78
831
1520





SORBIDRAFT_10g026200





[Sorghum bicolor]





> gi|241915621|gb|EER88765.1|





hypothetical protein





SORBIDRAFT_10g026200





[Sorghum bicolor]




ACF78942
unknown [Zea mays]
0.70
832
1521





> gi|194690438|gb|ACF79303.1|





unknown [Zea mays]





> gi|223974801|gb|ACN31588.1|





unknown [Zea mays]




NP_001105656
SBP-domain protein4 [Zea
0.69
833
1522





mays]





> gi|5931784|emb|CAB56630.1|





SBP-domain protein 4





[Zea mays]



1925-1945
NP_001142263
hypothetical protein
1.00
834
1523





LOC100274432 [Zea mays]





> gi|194707898|gb|ACF88033.1|





unknown [Zea mays]




XP_002468497
hypothetical protein
0.81
835
1524





SORBIDRAFT_01g046950





[Sorghum bicolor]





> gi|241922351|gb|EER95495.1|





hypothetical protein





SORBIDRAFT_01g046950





[Sorghum bicolor]



 88-108
XP_002448249
hypothetical protein
1.00
836
1525





SORBIDRAFT_06g023950





[Sorghum bicolor]





> gi|241939432|gb|EES12577.1|





hypothetical protein





SORBIDRAFT_06g023950





[Sorghum bicolor]




NP_001141834
hypothetical protein
0.92
837
1526





LOC100273976 [Zea mays]





> gi|194706118|gb|ACF87143.1|





unknown [Zea mays]




BAJ88132
predicted protein [Hordeum
0.86
838
1527





vulgare subsp. vulgare]




CAE02784
OSJNBa0011L07.8 [Oryza
0.87
839
1528





sativa Japonica Group]





> gi|116310379|emb|CAH67390.1|





H0115B09.2 [Oryza





sativa Indica Group]





> gi|215768681|dbj|BAH00910.1|





unnamed protein





product [Oryza sativa Japonica





Group]





> gi|218195296|gb|EEC77723.1|





hypothetical protein





OsI_16815 [Oryza sativa





Indica Group]





> gi|222629289|gb|EEE61421.1|





hypothetical protein





OsJ_15623 [Oryza sativa





Japonica Group]




ACF88110
unknown [Zea mays]
0.76
840
1529




NP_001065045
Os10g0512700 [Oryza sativa
0.73
841
1530





Japonica Group]





> gi|78708906|gb|ABB47881.1|





SH3 domain-containing





protein 3, putative, expressed





[Oryza sativa Japonica Group]





> gi|113639654|dbj|BAF26959.1|





Os10g0512700 [Oryza





sativa Japonica Group]





> gi|218184876|gb|EEC67303.1|





hypothetical protein





OsI_34298 [Oryza sativa





Indica Group]





> gi|222613131|gb|EEE51263.1|





hypothetical protein





OsJ_32141 [Oryza sativa





Japonica Group]




BAJ89635
predicted protein [Hordeum
0.72
842
1531





vulgare subsp. vulgare]




ACG28140
clathrin binding protein [Zea
0.72
843
1532





mays]




NP_001130175
hypothetical protein
0.72
844
1533





LOC100191269 [Zea mays]





> gi|194688470|gb|ACF78319.1|





unknown [Zea mays]




XP_002464381
hypothetical protein
0.71
845
1534





SORBIDRAFT_01g017250





[Sorghum bicolor]





> gi|241918235|gb|EER91379.1|





hypothetical protein





SORBIDRAFT_01g017250





[Sorghum bicolor]



2810-2830
XP_002458234
hypothetical protein
1.00
846
1535





SORBIDRAFT_03g029520





[Sorghum bicolor]





> gi|241930209|gb|EES03354.1|





hypothetical protein





SORBIDRAFT_03g029520





[Sorghum bicolor]




CAI77627
potassium uptake channel [Zea
0.93
847





mays]




NP_001105480
potassium channel5 [Zea
0.91
848
1536





mays]





> gi|2104908|emb|CAA68912.1|





potassium channel [Zea





mays]




NP_001043713
Os01g0648000 [Oryza sativa
0.82
849
1537





Japonica Group]





> gi|122241153|sp|Q0JKV1.1|





AKT1_ORYSJ RecName:





Full = Potassium channel





AKT1; Short = OsAKT1





> gi|113533244|dbj|BAF05627.1|





Os01g0648000 [Oryza





sativa Japonica Group]




P0C550
RecName: Full = Potassium
0.82
850





channel AKT1;





Short = OsAKT1




ABE99810
inwardly rectifying potassium
0.81
851
1538





channel AKT1 [Hordeum





vulgare]





> gi|326519172|dbj|BAJ96585.1|





predicted protein





[Hordeum vulgare subsp.





vulgare]




AAF36832
AKT1-like potassium channel
0.81
852
1539





[Triticum aestivum]




AAL40894
AKT1-like potassium channel
0.78
853
1540





[Oryza sativa]




ADK93728
inward-rectifying potassium
0.79
854
1541





channel [Puccinellia





tenuiflora]




EEE55080
hypothetical protein
0.78
855





OsJ_02815 [Oryza sativa





Japonica Group]



1465-1485
NP_001151754
collagen, type IV, alpha 5 [Zea
1.00
856
1542





mays]





> gi|195649521|gb|ACG44228.1|





collagen, type IV, alpha





5 [Zea mays]




XP_002460574
hypothetical protein
0.87
857
1543





SORBIDRAFT_02g031070





[Sorghum bicolor]





> gi|241923951|gb|EER97095.1|





hypothetical protein





SORBIDRAFT_02g031070





[Sorghum bicolor]



1702-1722
XP_002460183
hypothetical protein
1.00
858
1544





SORBIDRAFT_02g024090





[Sorghum bicolor]





> gi|241923560|gb|EER96704.1|





hypothetical protein





SORBIDRAFT_02g024090





[Sorghum bicolor]




NP_001149599
LOC100283225 [Zea mays]
0.96
859
1545





> gi|194705944|gb|ACF87056.1|





unknown [Zea mays]





> gi|195628360|gb|ACG36010.1|





annexin A4 [Zea mays]




ACG40326
annexin A4 [Zea mays]
0.95
860
1546




NP_001063096
Os09g0394900 [Oryza sativa
0.89
861
1547





Japonica Group]





> gi|49389155|dbj|BAD26449.1|





putative annexin [Oryza





sativa Japonica Group]





> gi|49389211|dbj|BAD26499.1|





putative annexin [Oryza





sativa Japonica Group]





> gi|113631329|dbj|BAF25010.1|





Os09g0394900 [Oryza





sativa Japonica Group]





> gi|215692709|dbj|BAG88129.1|





unnamed protein





product [Oryza sativa Japonica





Group]





> gi|215704491|dbj|BAG93925.1|





unnamed protein





product [Oryza sativa Japonica





Group]





> gi|222641513|gb|EEE69645.1|





hypothetical protein





OsJ_29255 [Oryza sativa





Japonica Group]




EEC84533
hypothetical protein
0.89
862





OsI_31267 [Oryza sativa





Indica Group]




NP_001132118
hypothetical protein
0.86
863
1548





LOC100193535 [Zea mays]





> gi|238908661|gb|ACF80822.2|





unknown [Zea mays]




BAJ85227
predicted protein [Hordeum
0.73
864
1549





vulgare subsp. vulgare]





> gi|326510845|dbj|BAJ91770.1|





predicted protein





[Hordeum vulgare subsp.





vulgare]




NP_001147343
annexin A4 [Zea mays]
0.72
865
1550





> gi|195610314|gb|ACG26987.1|





annexin A4 [Zea mays]





> gi|219887403|gb|ACL54076.1|





unknown [Zea mays]




AAR25142
annexin [Triticum aestivum]
0.73
866
1551




ACF06448
annexin [Elaeis guineensis]
0.74
867
1552


Predicted
891-914
ACG34432
L-allo-threonine aldolase [Zea
1.00
868
1553


folded


mays]


24-nts-


long seq


51802




ACG33933
L-allo-threonine aldolase [Zea
0.99
869
1554





mays]




NP_001149239
L-allo-threonine aldolase [Zea
0.98
870
1555





mays]





> gi|195625716|gb|ACG34688.1|





L-allo-threonine aldolase





[Zea mays]




NP_001053314
Os04g0516600 [Oryza sativa
0.79
871
1556





Japonica Group]





> gi|38344656|emb|CAD4182.21|





OSJNBb0072M01.18





[Oryza sativa Japonica Group]





> gi|113564885|dbj|BAF15228.1|





Os04g0516600 [Oryza





sativa Japonica Group]





> gi|116310209|emb|CAH67219.1|





OSIGBa0145M07.1





[Oryza sativa Indica Group]





> gi|215708770|dbj|BAG94039.1|





unnamed protein





product [Oryza sativa Japonica





Group]





> gi|215765369|dbj|BAG87066.1|





unnamed protein





product [Oryza sativa Japonica





Group]





> gi|218195217|gb|EEC77644.1|





hypothetical protein





OsI_16646 [Oryza sativa





Indica Group]





> gi|222629209|gb|EEE61341.1|





hypothetical protein





OsJ_15466 [Oryza sativa





Japonica Group]




CAE05443
OSJNBa0073E02.3 [Oryza
0.75
872
1557





sativa Japonica Group]




XP_002448187
hypothetical protein
0.79
873
1558





SORBIDRAFT_06g022630





[Sorghum bicolor]





> gi|241939370|gb|EES12515.1|





hypothetical protein





SORBIDRAFT_06g022630





[Sorghum bicolor]




ACG36649
L-allo-threonine aldolase [Zea
0.80
874
1559





mays]




BAK03835
predicted protein [Hordeum
0.79
875
1560





vulgare subsp. vulgare]




BAJ93201
predicted protein [Hordeum
0.76
876
1561





vulgare subsp. vulgare]




NP_001140767
hypothetical protein
0.72
877
1562





LOC100272842 [Zea mays]





> gi|194700996|gb|ACF84582.1|





unknown [Zea mays]



700-723
AAS47590
phospholipid-hydroperoxide
1.00
878
1563





glutathione peroxidase [Setaria





italica]




XP_002446921
hypothetical protein
0.99
879
1564





SORBIDRAFT_06g024920





[Sorghum bicolor]





> gi|48374968|gb|AAT42166.1|





putative glutathione





peroxidase [Sorghum bicolor]





> gi|241938104|gb|EES11249.1|





hypothetical protein





SORBIDRAFT_06g024920





[Sorghum bicolor]




NP_001141210
hypothetical protein
0.96
880
1565





LOC100273297 [Zea mays]





> gi|48374955|gb|AAT42154.1|





putative glutathione





peroxidase [Zea mays]





> gi|194703274|gb|ACF85721.1|





unknown [Zea mays]





> gi|195622840|gb|ACG33250.1|





phospholipid





hydroperoxide glutathione





peroxidase [Zea mays]





> gi|223975959|gb|ACN32167.1|





unknown [Zea mays]




ACG39625
phospholipid hydroperoxide
0.96
881
1566





glutathione peroxidase [Zea





mays]




EEC77777
hypothetical protein
0.95
882





OsI_16938 [Oryza sativa





Indica Group]




NP_001146472
hypothetical protein
0.94
883
1567





LOC100280060 [Zea mays]





> gi|219887431|gb|ACL54090.1|





unknown [Zea mays]




NP_001147681
phospholipid hydroperoxide
0.94
884
1568





glutathione peroxidase [Zea





mays]





> gi|195613068|gb|ACG28364.1|





phospholipid





hydroperoxide glutathione





peroxidase [Zea mays]




NP_001053524
Os04g0556300 [Oryza sativa
0.94
885
1569





Japonica Group]





> gi|21360380|gb|AAM47493.1|





glutathione peroxidase 1





[Oryza sativa]





> gi|113565095|dbj|BAF15438.1|





Os04g0556300 [Oryza





sativa Japonica Group]





> gi|215693018|dbj|BAG88438.1|





unnamed protein





product [Oryza sativa Japonica





Group]





> gi|222629338|gb|EEE61470.1|





hypothetical protein





OsJ_15735 [Oryza sativa





Japonica Group]




NP_001105091
GP protein [Zea mays]
0.94
886
1570





> gi|22268405|gb|AAM88847.2|





AF520911_1 putative





glutathione peroxidase [Zea





mays]




AAQ64633
cytosolic glutathione
0.93
887
1571





peroxidase [Triticum





monococcum]



714-737
XP_002446921
hypothetical protein
1.00
888
1572





SORBIDRAFT_06g024920





[Sorghum bicolor]





> gi|48374968|gb|AAT42166.1|





putative glutathione





peroxidase [Sorghum bicolor]





> gi|241938104|gb|EES11249.1|





hypothetical protein





SORBIDRAFT_06g024920





[Sorghum bicolor]




AAS47590
phospholipid-hydroperoxide
0.99
889
1573





glutathione peroxidase [Setaria





italica]




NP_001141210
hypothetical protein
0.98
890
1574





LOC100273297 [Zea mays]





> gi|48374955|gb|AAT42154.1|





putative glutathione





peroxidase [Zea mays]





> gi|194703274|gb|ACF85721.1|





unknown [Zea mays]





> gi|195622840|gb|ACG33250.1|





phospholipid





hydroperoxide glutathione





peroxidase [Zea mays]





> gi|223975959|gb|ACN32167.1|





unknown [Zea mays]




ACG39625
phospholipid hydroperoxide
0.97
891
1575





glutathione peroxidase [Zea





mays]




EEC77777
hypothetical protein
0.96
892





OsI_16938 [Oryza sativa





Indica Group]




NP_001146472
hypothetical protein
0.95
893
1576





LOC100280060 [Zea mays]





> gi|219887431|gb|ACL54090.1|





unknown [Zea mays]




NP_001147681
phospholipid hydroperoxide
0.95
894
1577





glutathione peroxidase [Zea





mays]





> gi|195613068|gb|ACG28364.1|





phospholipid





hydroperoxide glutathione





peroxidase [Zea mays]




NP_001053524
Os04g0556300 [Oryza sativa
0.95
895
1578





Japonica Group]





> gi|21360380|gb|AAM47493.1|





glutathione peroxidase 1





[Oryza sativa]





> gi|113565095|dbj|BAF15438.1|





Os04g0556300 [Oryza





sativa Japonica Group]





> gi|215693018|dbj|BAG88438.1|





unnamed protein





product [Oryza sativa Japonica





Group]





> gi|222629338|gb|EEE61470.1|





hypothetical protein





OsJ_15735 [Oryza sativa





Japonica Group]




NP_001105091
GP protein [Zea mays]
0.95
896
1579





> gi|22268405|gb|AAM88847.2|





AF520911_1 putative





glutathione peroxidase [Zea





mays]




AAQ64633
cytosolic glutathione
0.95
897
1580





peroxidase [Triticum





monococcum]



160-183
NP_001159179
hypothetical protein
1.00
898
1581





LOC100304264 [Zea mays]





> gi|223942501|gb|ACN25334.1|





unknown [Zea mays]




NP_001105290
LOC542207 [Zea mays]
0.74
899
1582





> gi|57791232|gb|AAW56446.1|





methyl-binding domain





protein MBD113 [Zea mays]



1054-1077
XP_002450144
hypothetical protein
1.00
900
1583





SORBIDRAFT_05g001160





[Sorghum bicolor]





> gi|241935987|gb|EES09132.1|





hypothetical protein





SORBIDRAFT_05g001160





[Sorghum bicolor]




NP_001065992
Os12g0116000 [Oryza sativa
0.89
901
1584





Japonica Group]





> gi|108862099|gb|ABA96293.2|





Vacuolar sorting receptor





1 precursor, putative,





expressed [Oryza sativa





Japonica Group]





> gi|113648499|dbj|BAF29011.1|





Os12g0116000 [Oryza





sativa Japonica Group]





> gi|215694863|dbj|BAG90054.1|





unnamed protein





product [Oryza sativa Japonica





Group]




EEC68730
hypothetical protein
0.89
902





OsI_37233 [Oryza sativa





Indica Group]




EEE51529
hypothetical protein
0.89
903





OsJ_32728 [Oryza sativa





Japonica Group]




EEC67546
hypothetical protein
0.89
904





OsI_34875 [Oryza sativa





Indica Group]




BAK03786
predicted protein [Hordeum
0.87
905
1585





vulgare subsp. vulgare]




BAK00302
predicted protein [Hordeum
0.87
906
1586





vulgare subsp. vulgare]




CBI18031
unnamed protein product
0.78
907





[Vitis vinifera]




XP_002267833
PREDICTED: hypothetical
0.78
908
1587





protein [Vitis vinifera]




XP_002309184
predicted protein [Populus
0.78
909
1588





trichocarpa]





> gi|222855160|gb|EEE92707.1|





predicted protein





[Populus trichocarpa]



1792-1815
NP_001140790
hypothetical protein
1.00
910
1589





LOC100272865 [Zea mays]





> gi|194701088|gb|ACF84628.1|





unknown [Zea mays]




XP_002464219
hypothetical protein
0.85
911
1590





SORBIDRAFT_01g014350





[Sorghum bicolor]





> gi|241918073|gb|EER91217.1|





hypothetical protein





SORBIDRAFT_01g014350





[Sorghum bicolor]




XP_002464218
hypothetical protein
0.84
912
1591





SORBIDRAFT_01g014340





[Sorghum bicolor]





> gi|241918072|gb|EER91216.1|





hypothetical protein





SORBIDRAFT_01g014340





[Sorghum bicolor]




AAL73972
putative cytochrome P450-like
0.73
913
1592





protein [Sorghum bicolor]



2477-2500
XP_002454227
hypothetical protein
1.00
914
1593





SORBIDRAFT_04g027100





[Sorghum bicolor]





> gi|241934058|gb|EES07203.1|





hypothetical protein





SORBIDRAFT_04g027100





[Sorghum bicolor]




NP_001169751
hypothetical protein
0.93
915
1594





LOC100383632 [Zea mays]





> gi|224031415|gb|ACN34783.1|





unknown [Zea mays]




BAJ95386
predicted protein [Hordeum
0.78
916
1595





vulgare subsp. vulgare]




EEC73665
hypothetical protein
0.77
917





OsI_08209 [Oryza sativa





Indica Group]




NP_001047529
Os02g0637800 [Oryza sativa
0.77
918
1596





Japonica Group]





> gi|49387984|dbj|BAD25092.1|





unknown protein [Oryza





sativa Japonica Group]





> gi|49388709|dbj|BAD25890.1|





unknown protein [Oryza





sativa Japonica Group]





> gi|113537060|dbj|BAF09443.1|





Os02g0637800 [Oryza





sativa Japonica Group]





> gi|215704166|dbj|BAG93006.1|





unnamed protein





product [Oryza sativa Japonica





Group]





> gi|222623311|gb|EEE57443.1|





hypothetical protein





OsJ_07658 [Oryza sativa





Japonica Group]



3108-3131
NP_001169691
hypothetical protein
1.00
919
1597





LOC100383572 [Zea mays]





> gi|224030935|gb|ACN34543.1|





unknown [Zea mays]




ACN33688
unknown [Zea mays]
0.96
920
1598




NP_001131248
hypothetical protein
0.81
921
1599





LOC100192560 [Zea mays]





> gi|195656461|gb|ACG47698.1|





nucleic acid binding





protein [Zea mays]




XP_002441761
hypothetical protein
0.80
922
1600





SORBIDRAFT_08g001980





[Sorghum bicolor]





> gi|241942454|gb|EES15599.1|





hypothetical protein





SORBIDRAFT_08g001980





[Sorghum bicolor]



2192-2215
NP_001150353
LOC100283983 [Zea mays]
1.00
923
1601





> gi|195638608|gb|ACG38772.1|





ubiquilin-1 [Zea mays]





> gi|195644430|gb|ACG41683.1|





ubiquilin-1 [Zea mays]





> gi|224029247|gb|ACN33699.1|





unknown [Zea mays]




XP_002465921
hypothetical protein
0.92
924
1602





SORBIDRAFT_01g048260





[Sorghum bicolor]





> gi|241919775|gb|EER92919.1|





hypothetical protein





SORBIDRAFT_01g048260





[Sorghum bicolor]




NP_001048862
Os03g0131300 [Oryza sativa
0.78
925
1603





Japonica Group]





> gi|108706017|gb|ABF93812.1|





Ubiquitin family protein,





expressed [Oryza sativa





Japonica Group]





> gi|113547333|dbj|BAF10776.1|





Os03g0131300 [Oryza





sativa Japonica Group]





> gi|215713564|dbj|BAG94701.1|





unnamed protein





product [Oryza sativa Japonica





Group]





> gi|222624144|gb|EEE58276.1|





hypothetical protein





OsJ_09294 [Oryza sativa





Japonica Group]




EEC74450
hypothetical protein
0.78
926





OsI_09858 [Oryza sativa





Indica Group]




NP_001169509
hypothetical protein
0.78
927
1604





LOC100383383 [Zea mays]





> gi|224029753|gb|ACN33952.1|





unknown [Zea mays]




EAY79386
hypothetical protein
0.71
928





OsI_34513 [Oryza sativa





Indica Group]




N_001065193
Os10g0542200 [Oryza sativa
0.71
929
1605





Japonica Group]





> gi|10140758|gb|AAG13589.1|





AC051633_5 putative





ubiquitin protein [Oryza sativa





Japonica Group]





> gi|31433330|gb|AAP54859.1|





Ubiquitin family protein,





expressed [Oryza sativa





Japonica Group]





> gi|113639802|dbj|BAF27107.1|





Os10g0542200 [Oryza





sativa Japonica Group]




EEE51337
hypothetical protein
0.71
930





OsJ_32327 [Oryza sativa





Japonica Group]




BAJ87273
predicted protein [Hordeum
0.73
931
1606





vulgare subsp. vulgare]




BAK06552
predicted protein [Hordeum
0.74
932
1607





vulgare subsp. vulgare]



1289-1312
NP_001046031
Os02g0171100 [Oryza sativa
1.00
933
1608





Japonica Group]





> gi|49387565|dbj|BAD25496.1|





putative glyceraldehyde-





3-phosphate dehydrogenase





[Oryza sativa Japonica Group]





> gi|49388082|dbj|BAD25194.1|





putative glyceraldehyde-





3-phosphate dehydrogenase





[Oryza sativa Japonica Group]





> gi|113535562|dbj|BAF07945.1|





Os02g0171100 [Oryza





sativa Japonica Group]





> gi|215697739|dbj|BAG91733.1|





unnamed protein





product [Oryza sativa Japonica





Group]





> gi|218190154|gb|EEC72581.1|





hypothetical protein





OsI_06028 [Oryza sativa





Indica Group]





> gi|222622270|gb|EEE56402.1|





hypothetical protein





OsJ_05558 [Oryza sativa





Japonica Group]




NP_001149357
LOC100282981 [Zea mays]
0.92
934
1609





> gi|195626622|gb|ACG35141.1|





glyceraldehyde-3-





phosphate dehydrogenase,





cytosolic [Zea mays]





> gi|195641440|gb|ACG40188.1|





glyceraldehyde-3-





phosphate dehydrogenase,





cytosolic [Zea mays]




ACL54292
unknown [Zea mays]
0.92
935
1610




XP_002451619
hypothetical protein
0.90
936
1611





SORBIDRAFT_04g004750





[Sorghum bicolor]





> gi|241931450|gb|EES04595.1|





hypothetical protein





SORBIDRAFT_04g004750





[Sorghum bicolor]




NP_001130796
hypothetical protein
0.88
937
1612





LOC100191900 [Zea mays]





> gi|194690136|gb|ACF79152.1|





unknown [Zea mays]





> gi|195622606|gb|ACG33133.1|





glyceraldehyde-3-





phosphate dehydrogenase,





cytosolic [Zea mays]





> gi|195625996|gb|ACG34828.1|





glyceraldehyde-3-





phosphate dehydrogenase,





cytosolic [Zea mays]




NP_001058309
Os06g0666600 [Oryza sativa
0.85
938
1613





Japonica Group]





> gi|52076528|dbj|BAD45405.1|





putative glyceraldehyde-





3-phosphate dehydrogenase





[Oryza sativa Japonica Group]





> gi|113596349|dbj|BAF20223.1|





Os06g0666600 [Oryza





sativa Japonica Group]





> gi|125556399|gb|EAZ02005.1|





hypothetical protein





OsI_24036 [Oryza sativa





Indica Group]





> gi|215741351|dbj|BAG97846.1|





unnamed protein





product [Oryza sativa Japonica





Group]




BAJ86344
predicted protein [Hordeum
0.82
939
1614





vulgare subsp. vulgare]





> gi|326520716|dbj|BAJ92721.1|





predicted protein





[Hordeum vulgare subsp.





vulgare]




ADJ96634
glyceraldehyde-3-phosphate
0.82
940
1615





dehydrogenase [Guzmania





wittmackii x Guzmania





lingulata]




CAC80377
glyceraldehyde-3-phosphate
0.77
941
1616





dehydrogenase [Capsicum





annuum]





> gi|26986729|emb|CAC88118.1|





glyceraldehyde-3-





phosphate dehydrogenase





[Capsicum annuum]




XP_002263263
PREDICTED: hypothetical
0.75
942
1617





protein [Vitis vinifera]





> gi|297736130|emb|CBI24168.3|





unnamed protein





product [Vitis vinifera]


Predicted
173-196
AAL76004
putative gag-pol polyprotein
1.00
943


folded


[Zea mays]


24-nts-


long seq


52801




AAN40035
putative gag-pol polyprotein
0.90
944





[Zea mays]




AAN40025
putative gag-pol polyprotein
0.85
945





[Zea mays]




AAL66753
putative copia-type pol
0.81
946





polyprotein [Zea mays]




AAP94599
putative copia-type pol
0.81
947





polyprotein [Zea mays]


Predicted
14-37
XP_002458660
hypothetical protein
1.00
948
1618


folded


SORBIDRAFT_03g037610


24-nts-


[Sorghum bicolor]


long seq


> gi|241930635|gb|EES03780.1|


52452


hypothetical protein





SORBIDRAFT_03g037610





[Sorghum bicolor]




NP_001106055
ribonuclease III domain
0.98
949
1619





protein1 [Zea mays]





> gi|149980667|gb|ABR53724.1|





chloroplast ribonuclease





III domain protein [Zea mays]





> gi|194704042|gb|ACF86105.1|





unknown [Zea mays]





> gi|195647406|gb|ACG43171.1|





RNA binding protein





[Zea mays]





> gi|223950477|gb|ACN29322.1|





unknown [Zea mays]




BAJ85766
predicted protein [Hordeum
0.91
950
1620





vulgare subsp. vulgare]





> gi|326503490|dbj|BAJ86251.1|





predicted protein





[Hordeum vulgare subsp.





vulgare]





> gi|326520661|dbj|BAJ92694.1|





predicted protein





[Hordeum vulgare subsp.





vulgare]




EEE55556
hypothetical protein
0.89
951





OsJ_03820 [Oryza sativa





Japonica Group]




NP_001044581
Os01g0810100 [Oryza sativa
0.92
952
1621





Japonica Group]





> gi|20160696|dbj|BAB89639.1|





unknown protein [Oryza





sativa Japonica Group]





> gi|113534112|dbj|BAF06495.1|





Os01g0810100 [Oryza





sativa Japonica Group]





> gi|215701330|dbj|BAG92754.1|





unnamed protein





product [Oryza sativa Japonica





Group]





> gi|215704464|dbj|BAG93898.1|





unnamed protein





product [Oryza sativa Japonica





Group]




EEC71679
hypothetical protein
0.86
953





OsI_04153 [Oryza sativa





Indica Group]




BAJ91324
predicted protein [Hordeum
0.85
954
1622





vulgare subsp. vulgare]




XP_002533159
RNA binding protein, putative
0.75
955
1623





[Ricinus communis]





> gi|223527031|gb|EEF29218.1|





RNA binding protein,





putative [Ricinus communis]




NP_195467
Ribonuclease III family
0.74
956
1624





protein [Arabidopsis thaliana]





> gi|4468817|emb|CAB38218.1|





putative protein





[Arabidopsis thaliana]





> gi|7270733|emb|CAB80416.1|





putative protein





[Arabidopsis thaliana]





> gi|95147308|gb|ABF57289.1|





At4g37510 [Arabidopsis





thaliana]





> gi|332661403|gb|AEE86803.1|





Ribonuclease III family





protein [Arabidopsis thaliana]




XP_002866942
ribonuclease III family protein
0.74
957
1625





[Arabidopsis lyrata subsp.





lyrata]





> gi|297312778|gb|EFH43201.1|





ribonuclease III family





protein [Arabidopsis lyrata





subsp. lyrata]



 86-109
NP_001169273
hypothetical protein
1.00
958
1626





LOC100383136 [Zea mays]





> gi|224028313|gb|ACN33232.1|





unknown [Zea mays]




XP_022462951
hypothetical protein
0.94
959
1627





SORBIDRAFT_02g035180





[Sorghum bicolor]





> gi|241926328|gb|EER99472.1|





hypothetical protein





SORBIDRAFT_02g035180





[Sorghum bicolor]




BAK06716
predicted protein [Hordeum
0.82
960
1628





vulgare subsp. vulgare]




EEC82174
hypothetical protein
0.81
961





OsI_26284 [Oryza sativa





Indica Group]




NP_001059844
Os07g0530100 [Oryza sativa
0.81
962
1629





Japonica Group]





> gi|33146648|dbj|BAC79984.1|





putative tyrosyl-DNA





phosphodiesterase [Oryza





sativa Japonica Group]





> gi|113611380|dbj|BAF21758.1|





Os07g0530100 [Oryza





sativa Japonica Group]





> gi|215697362|dbj|BAG91356.1|





unnamed protein





product [Oryza sativa Japonica





Group]





> gi|222637174|gb|EEE67306.1|





hypothetical protein





OsJ_24533 [Oryza sativa





Japonica Group]


Predicted
69-89
NP_001167972
hypothetical protein
1.00


zma


LOC100381689 [Zea mays]


mir


> gi|223945237|gb|ACN26702.1|


48588


unknown [Zea mays]


zma-
1395-1415
NP_001151161
riboflavin kinase family
1.00
963
1630


miR166d*


protein [Zea mays]





> gi|195644710|gb|ACG41823.1|





riboflavin kinase family





protein [Zea mays]




EEC76352
hypothetical protein
0.78
964





OsI_13934 [Oryza sativa





Indica Group]




ABF99395
riboflavin biosynthesis protein,
0.77
965
1631





putative, expressed [Oryza





sativa Japonica Group]




NP_001051594
Os03g0801700 [Oryza sativa
0.77
966
1632





Japonica Group]





> gi|108711599|gb|ABF99394.1|





riboflavin biosynthesis





protein, putative, expressed





[Oryza sativa Japonica Group]





> gi|113550065|dbj|BAF13508.1|





Os03g0801700 [Oryza





sativa Japonica Group]





> gi|222625981|gb|EEE60113.1|





hypothetical protein





OsJ_12987 [Oryza sativa





Japonica Group]




BAK03408
predicted protein [Hordeum
0.73
967
1633





vulgare subsp. vulgare]




BAJ90790
predicted protein [Hordeum
0.72
968
1634





vulgare subsp. vulgare]


Predicted
923-946
XP_002461133
hypothetical protein
1.00
969
1635


folded


SORBIDRAFT_02g041270


24-nts-


[Sorghum bicolor]


long seq


> gi|241924510|gb|EER97654.1|


52795


hypothetical protein





SORBIDRAFT_02g041270





[Sorghum bicolor]




ACR36108
unknown [Zea mays]
0.97
970
1636




NP_001149600
protein SFT2 [Zea mays]
0.96
971
1637





> gi|195628366|gb|ACG36013.1|





protein SFT2 [Zea mays]




BAJ93024
predicted protein [Hordeum
0.90
972
1638





vulgare subsp. vulgare]




BAJ93185
predicted protein [Hordeum
0.90
973
1639





vulgare subsp. vulgare]




NP_001057449
Os06g0300300 [Oryza sativa
0.89
974
1640





Japonica Group]





> gi|53792521|dbj|BAD53485.1|





unknown protein [Oryza





sativa Japonica Group]





> gi|113595489|dbj|BAF19363.1|





Os06g0300300 [Oryza





sativa Japonica Group]





> gi|215697111|dbj|BAG91105.1|





unnamed protein





product [Oryza sativa Japonica





Group]





> gi|215766579|dbj|BAG98738.1|





unnamed protein





product [Oryza sativa Japonica





Group]





> gi|218198021|gb|EEC80448.1|





hypothetical protein





OsI_22649 [Oryza sativa





Indica Group]





> gi|222635438|gb|EEE65570.1|





hypothetical protein





OsJ_21068 [Oryza sativa





Japonica Group]




XP_002532175
Protein transport protein SFT2,
0.73
975
1641





putative [Ricinus communis]





> gi|223528143|gb|EEF30212.1|





Protein transport protein





SFT2, putative [Ricinus





communis]




XP_002264182
PREDICTED: hypothetical
0.70
976
1642





protein [Vitis vinifera]





> gi|296085064|emb|CBI28479.3|





unnamed protein





product [Vitis vinifera]



149-172
XP_002445951
hypothetical protein
1.00
977
1643





SORBIDRAFT_07g028610





[Sorghum bicolor]





> gi|241942301|gb|EES15446.1|





hypothetical protein





SORBIDRAFT_07g028610





[Sorghum bicolor]




NP_001131191
hypothetical protein
0.92
978
1644





LOC100192499 [Zea mays]





> gi|194690830|gb|ACF79499.1|





unknown [Zea mays]




BAK06465
predicted protein [Hordeum
0.83
979
1645





vulgare subsp. vulgare]





> gi|326515200|dbj|BAK03513.1|





predicted protein





[Hordeum vulgare subsp.





vulgare]




EAZ07476
hypothetical protein
0.83
980





OsI_29735 [Oryza sativa





Indica Group]





> gi|125603873|gb|EAZ43198.1|





hypothetical protein





OsJ_27795 [Oryza sativa





Japonica Group]




NP_001062134
Os08g0496000 [Oryza sativa
0.83
981
1646





Japonica Group]





> gi|42408865|dbj|BAD10124.1|





putative cytochrome c





oxidase subunit 15(COX15)





homolog isoform 1 precursor





[Oryza sativa Japonica Group]





> gi|113624103|dbj|BAF24048.1|





Os08g0496000 [Oryza





sativa Japonica Group]





> gi|215737013|dbj|BAG95942.1|





unnamed protein





product [Oryza sativa Japonica





Group]


zma-
107-128
XP_002451684
hypothetical protein
1.00
992
1655


miR169c*


SORBIDRAFT_04g005850





[Sorghum bicolor]





> gi|241931515|gb|EES04660.1|





hypothetical protein





SORBIDRAFT_04g005850





[Sorghum bicolor]




NP_001169303
hypothetical protein
0.85
993
1656





LOC100383167 [Zea mays]





> gi|195627360|gb|ACG35510.1|





cytochrome P450





CYP71K14 [Zea mays]




ACN33338
unknown [Zea mays]
0.76
994
1657



1135-1156
NP_001169303
hypothetical protein
1.00
995
1658





LOC100383167 [Zea mays]





> gi|195627360|gb|ACG35510.1|





cytochrome P450





CYP71K14 [Zea mays]




ACN33338
unknown [Zea mays]
0.88
996
1659




XP_002451684
hypothetical protein
0.85
997
1660





SORBIDRAFT_04g005850





[Sorghum bicolor]





> gi|241931515|gb|EES04660.1|





hypothetical protein





SORBIDRAFT_04g005850





[Sorghum bicolor]


Predicted
606-625
XP_002436376
hypothetical protein
1.00
998
1661


zma


SORBIDRAFT_10g001390


mir


[Sorghum bicolor]


50517


> gi|241914599|gb|EER87743.1|





hypothetical protein





SORBIDRAFT_10g001390





[Sorghum bicolor]




NP_001150208
DAG protein [Zea mays]
0.81
999
1662





> gi|195637572|gb|ACG38254.1|





DAG protein [Zea mays]





> gi|223947219|gb|ACN27693.1|





unknown [Zea mays]




BAC22214
putative plastid protein [Oryza
0.81
1000
1663





sativa Japonica Group]




BAK01694
predicted protein [Hordeum
0.80
1001
1664





vulgare subsp. vulgare]




BAJ87670
predicted protein [Hordeum
0.76
1002
1665





vulgare subsp. vulgare]



560-579
NP_001056622
Os06g0116600 [Oryza sativa
1.00
1003
1666





Japonica Group]





> gi|55296199|dbj|BAD67917.1|





putative DAL1 protein





[Oryza sativa Japonica Group]





> gi|113594662|dbj|BAF18536.1|





Os06g0116600 [Oryza





sativa Japonica Group]





> gi|218197457|gb|EEC79884.1|





hypothetical protein





OsI_21391 [Oryza sativa





Indica Group]





> gi|222634859|gb|EEE64991.1|





hypothetical protein





OsJ_19911 [Oryza sativa





Japonica Group]



3352-3371
NP_001170545
hypothetical protein
1.00
1004
1667





LOC100384563 [Zea mays]





> gi|238005944|gb|ACR34007.1|





unknown [Zea mays]




XP_002436375
hypothetical protein
0.85
1005
1668





SORBIDRAFT_10g001380





[Sorghum bicolor]





> gi|241914598|gb|EER87742.1|





hypothetical protein





SORBIDRAFT_10g001380





[Sorghum bicolor]




EEC79883
hypothetical protein
0.81
1006





OsI_21390 [Oryza sativa





Indica Group]




EEE64990
hypothetical protein
0.78
1007





OsJ_19910 [Oryza sativa





Japonica Group]




NP_001045600
Os02g0102800 [Oryza sativa
0.75
1008
1669





Japonica Group]





> gi|41052906|dbj|BAD07818.1|





unknown protein [Oryza





sativa Japonica Group]





> gi|41053240|dbj|BAD08201.1|





unknown protein [Oryza





sativa Japonica Group]





> gi|113535131|dbj|BAF07514.1|





Os02g0102800 [Oryza





sativa Japonica Group]


Predicted
965-985
NP_001141527
hypothetical protein
1.00
1009
1670


zma


LOC100273639 [Zea mays]


mir


> gi|194704936|gb|ACF86552.1|


49435


unknown [Zea mays]





> gi|195642904|gb|ACG40920.1|





hypothetical protein [Zea





mays]




XP_002440488
hypothetical protein
0.85
1010
1671





SORBIDRAFT_09g001840





[Sorghum bicolor]





> gi|241945773|gb|EES18918.1|





hypothetical protein





SORBIDRAFT_09g001840





[Sorghum bicolor]



358-378
ACF85023
unknown [Zea mays]
1.00
1011
1672




NP_001151177
transposon protein [Zea mays]
0.99
1012
1673





> gi|195644822|gb|ACG41879.1|





transposon protein [Zea





mays]




XP_002452722
hypothetical protein
0.86
1013
1674





SORBIDRAFT_04g031370





[Sorghum bicolor]





> gi|241932553|gb|EES05698.1|





hypothetical protein





SORBIDRAFT_04g031370





[Sorghum bicolor]




NP_001047784
Os02g0689500 [Oryza sativa
0.77
1014
1675





Japonica Group]





> gi|41052806|dbj|BAD07674.1|





unknown protein [Oryza





sativa Japonica Group]





> gi|113537315|dbj|BAF09698.1|





Os02g0689500 [Oryza





sativa Japonica Group]





> gi|125540730|gb|EAY87125.1|





hypothetical protein





OsI_08527 [Oryza sativa





Indica Group]





> gi|215704259|dbj|BAG93099.1|





unnamed protein





product [Oryza sativa Japonica





Group]


mtr-
174-194
XP_002468544
hypothetical protein
1.00
1015
1676


miR169q


SORBIDRAFT_01g047710





[Sorghum bicolor]





> gi|241922398|gb|EER95542.1|





hypothetical protein





SORBIDRAFT_01g047710





[Sorghum bicolor]




NP_001142595
hypothetical protein
0.85
1016
1677





LOC100274862 [Zea mays]





> gi|195607096|gb|ACG25378.1|





hypothetical protein [Zea





mays]




ACN31627
unknown [Zea mays]
0.85
1017
1678




ACR38267
unknown [Zea mays]
0.85
1018
1679




NP_001144209
hypothetical protein
0.85
1019
1680





LOC100277070 [Zea mays]





> gi|195638456|gb|ACG38696.1|





hypothetical protein [Zea





mays]




NP_001048909
Os03g0138500 [Oryza sativa
0.72
1020
1681





Japonica Group]





> gi|108706085|gb|ABF93880.1|





expressed protein [Oryza





sativa Japonica Group]





> gi|113547380|dbj|BAF10823.1|





Os03g0138500 [Oryza





sativa Japonica Group]




EAZ25513
hypothetical protein
0.71
1021





OsJ_09336 [Oryza sativa





Japonica Group]




EAY88465
hypothetical protein
0.71
1022





OsI_09934 [Oryza sativa





Indica Group]



1074-1094
NP_001150090
PIT1 [Zea mays]
1.00
1023
1682





> gi|195636634|gb|ACG37785.1|





PIT1 [Zea mays]




NP_001042834
Os01g0303600 [Oryza sativa
0.76
1024
1683





Japonica Group]





> gi|52075677|dbj|BAD44897.1|





zinc finger protein-like





[Oryza sativa Japonica Group]





> gi|52077476|dbj|BAD45040.1|





zinc finger protein-like





[Oryza sativa Japonica Group]





> gi|218188061|gb|EEC70488.1|





hypothetical protein





OsI_01559 [Oryza sativa





Indica Group]





> gi|255673151|dbj|BAF04748.2|





Os01g0303600 [Oryza





sativa Japonica Group]


Predicted
71-92
XP_002437560
hypothetical protein
1.00
1025
1684


zma


SORBIDRAFT_10g029400


mir


[Sorghum bicolor]


49003


> gi|241915783|gb|EER88927.1|





hypothetical protein





SORBIDRAFT_10g029400





[Sorghum bicolor]




ACL53917
unknown [Zea mays]
0.97
1026
1685




NP_001152313
LOC100285952 [Zea mays]
0.97
1027
1686





> gi|195654989|gb|ACG46962.1|





MPK17-1-putative





MAPK [Zea mays]




NP_001146196
LOC100279766 [Zea mays]
0.95
1028
1687





> gi|219886151|gb|ACL53450.1|





unknown [Zea mays]





> gi|297595201|gb|ADI48121.1|





putative mitogen-activated





protein kinase 17-3 [Zea mays]




EEC81289
hypothetical protein
0.91
1029





OsI_24409 [Oryza sativa





Indica Group]




NP_001058530
Os06g0708000 [Oryza sativa
0.91
1030
1688





Japonica Group]





> gi|108860803|sp|Q5Z9J0.2|





MPK12_ORYSJ RecName:





Full = Mitogen-activated





protein kinase 12; Short = MAP





kinase 12; AltName:





Full = Blast- and wound-





induced MAP kinase 1;





AltName: Full = MAP kinase 1;





AltName: Full = OsBWMK1;





AltName: Full = OsMAPK1





> gi|53792601|dbj|BAD53616.1|





putative MAP kinase





[Oryza sativa Japonica Group]





> gi|113596570|dbj|BAF20444.1|





Os06g0708000 [Oryza





sativa Japonica Group]




AAX20165
putative MAPK protein kinase
0.86
1031
1689





[Triticum aestivum]





> gi|84795226|gb|ABC65849.1|





mitogen-activated protein





kinase MAPK1e [Triticum





aestivum]




ABC54587
mitogen-activated protein
0.86
1032
1690





kinase [Triticum aestivum]




AAX20166
putative MAPK protein kinase
0.86
1033
1691





[Triticum aestivum]




ABD97883
mitogen-activated protein
0.86
1034
1692





kinase [Triticum aestivum]



53-74
EAY76957
hypothetical protein
1.00
1035





OsI_04915 [Oryza sativa





Indica Group]




NP_001045192
Os01g0916200 [Oryza sativa
1.00
1036
1693





Japonica Group]





> gi|19386749|dbj|BAB86130.1|





putative adapter-related





protein complex 4 epsilon 1





subunit [Oryza sativa Japonica





Group]





> gi|20805003|dbj|BAB92679.1|





putative adapter-related





protein complex 4 epsilon 1





subunit [Oryza sativa Japonica





Group]





> gi|113534723|dbj|BAF07106.1|





Os01g0916200 [Oryza





sativa Japonica Group]





> gi|215707205|dbj|BAG93665.1|





unnamed protein





product [Oryza sativa Japonica





Group]




EAZ14613
hypothetical protein
0.93
1037





OsJ_04538 [Oryza sativa





Japonica Group]




XP_002458982
hypothetical protein
0.91
1038
1694





SORBIDRAFT_03g043730





[Sorghum bicolor]





> gi|241930957|gb|EES04102.1|





hypothetical protein





SORBIDRAFT_03g043730





[Sorghum bicolor]




NP_001169710
hypothetical protein
0.90
1039
1695





LOC100383591 [Zea mays]





> gi|224031083|gb|ACN34617.1|





unknown [Zea mays]




BAJ89128
predicted protein [Hordeum
0.88
1040
1696





vulgare subsp. vulgare]




XP_002328755
predicted protein [Populus
0.70
1041
1697





trichocarpa]





> gi|222839053|gb|EEE77404.1|





predicted protein





[Populus trichocarpa]




XP_002519936
AP-1 complex subunit
0.71
1042
1698





gamma-2, putative [Ricinus





communis]





> gi|223540982|gb|EEF42540.1|





AP-1 complex subunit





gamma-2, putative [Ricinus





communis]


zma-
897-917
NP_001105061
Hageman factor inhibitor [Zea
1.00
1043
1699


miR399g


mays]





> gi|16305146|gb|AAL16995.1|





Hageman factor inhibitor





[Zea mays]




NP_001106233
trypsin/factor XIIA inhibitor
0.84
1044
1700





precursor [Zea mays]





> gi|266398|sp|P01088.2|ITRF_MAIZE





RecName:





Full = Trypsin/factor XIIA





inhibitor; AltName:





Full = CHFI; AltName:





Full = Hageman factor





inhibitor; Flags: Precursor





> gi|22327|emb|CAA37998.1|





corn Hageman factor





inhibitor [Zea mays]





> gi|75994173|gb|ABA34122.1|





hageman factor inhibitor





[Zea mays subsp. parviglumis]





> gi|75994177|gb|ABA34124.1|





hageman factor inhibitor





[Zea mays subsp. parviglumis]





> gi|75994189|gb|ABA34130.1|





hageman factor inhibitor





[Zea mays subsp. parviglumis]





> gi|75994193|gb|ABA34132.1|





hageman factor inhibitor





[Zea mays subsp. parviglumis]





> gi|195658559|gb|ACG48747.1|





trypsin/factor XIIA





inhibitor precursor [Zea mays]





> gi|195658619|gb|ACG48777.1|





trypsin/factor XIIA





inhibitor precursor [Zea mays]





> gi|214014768|gb|ACJ62034.1|





Hageman factor inhibitor





[Zea mays subsp. parviglumis]





> gi|214014812|gb|ACJ62056.1|





Hageman factor inhibitor





[Zea mays subsp. parviglumis]





> gi|214014814|gb|ACJ62057.1|





Hageman factor inhibitor





[Zea mays subsp. parviglumis]





> gi|214014820|gb|ACJ62060.1|





Hageman factor inhibitor





[Zea mays subsp. parviglumis]





> gi|214014826|gb|ACJ62063.1|





Hageman factor inhibitor





[Zea mays subsp. parviglumis]





> gi|214014828|gb|ACJ62064.1|





Hageman factor inhibitor





[Zea mays subsp. parviglumis]





> gi|214014862|gb|ACJ62081.1|





Hageman factor inhibitor





[Zea mays subsp. parviglumis]





> gi|214014864|gb|ACJ62082.1|





Hageman factor inhibitor





[Zea mays subsp. parviglumis]





> gi|214014870|gb|ACJ62085.1|





Hageman factor inhibitor





[Zea mays subsp. parviglumis]





> gi|238014562|gb|ACR38316.1|





unknown [Zea mays]




ABA34135
hageman factor inhibitor [Zea
0.83
1045
1701





mays subsp. parviglumis]





> gi|214014790|gb|ACJ62045.1|





Hageman factor inhibitor





[Zea mays subsp. parviglumis]





> gi|214014796|gb|ACJ62048.1|





Hageman factor inhibitor





[Zea mays subsp. parviglumis]




ACJ62011
Hageman factor inhibitor [Zea
0.83
1046
1702





mays subsp. parviglumis]





> gi|214014726|gb|ACJ62013.1|





Hageman factor inhibitor





[Zea mays subsp. parviglumis]





> gi|214014728|gb|ACJ62014.1|





Hageman factor inhibitor





[Zea mays subsp. parviglumis]





> gi|214014730|gb|ACJ62015.1|





Hageman factor inhibitor





[Zea mays subsp. parviglumis]





> gi|214014732|gb|ACJ62016.1|





Hageman factor inhibitor





[Zea mays subsp. parviglumis]





> gi|214014734|gb|ACJ62017.1|





Hageman factor inhibitor





[Zea mays subsp. parviglumis]





> gi|214014744|gb|ACJ62022.1|





Hageman factor inhibitor





[Zea mays subsp. parviglumis]





> gi|214014746|gb|ACJ62023.1|





Hageman factor inhibitor





[Zea mays subsp. parviglumis]





> gi|214014748|gb|ACJ62024.1|





Hageman factor inhibitor





[Zea mays subsp. parviglumis]




ABA34133
hageman factor inhibitor [Zea
0.83
1047
1703





mays subsp. parviglumis]




ACJ62080
Hageman factor inhibitor [Zea
0.83
1048
1704





mays subsp. parviglumis]




ABA34137
hageman factor inhibitor [Zea
0.83
1049
1705





diploperennis]





> gi|75994207|gb|ABA34139.1|





hageman factor inhibitor





[Zea diploperennis]





> gi|75994211|gb|ABA34141.1|





hageman factor inhibitor





[Zea diploperennis]





> gi|75994213|gb|ABA34142.1|





hageman factor inhibitor





[Zea diploperennis]





> gi|75994215|gb|ABA34143.1|





hageman factor inhibitor





[Zea diploperennis]





> gi|75994217|gb|ABA34144.1|





hageman factor inhibitor





[Zea diploperennis]




ACG48546
trypsin/factor XIIA inhibitor
0.83
1050
1706





precursor [Zea mays]




ABA34129
hageman factor inhibitor [Zea
0.83
1051
1707





mays subsp. parviglumis]




ABA34125
hageman factor inhibitor [Zea
0.83
1052
1708





mays subsp. parviglumis]





> gi|75994185|gb|ABA34128.1|





hageman factor inhibitor





[Zea mays subsp. parviglumis]





> gi|214014736|gb|ACJ62018.1|





Hageman factor inhibitor





[Zea mays subsp. parviglumis]





> gi|214014752|gb|ACJ62026.1|





Hageman factor inhibitor





[Zea mays subsp. parviglumis]





> gi|214014822|gb|ACJ62061.1|





Hageman factor inhibitor





[Zea mays subsp. parviglumis]


Predicted
379-399
XP_002454327
hypothetical protein
1.00
1053
1709


zma


SORBIDRAFT_04g028730


mir


[Sorghum bicolor]


49985


> gi|241934158|gb|EES07303.1|





hypothetical protein





SORBIDRAFT_04g028730





[Sorghum bicolor]




ACG36841
caleosin related protein [Zea
0.81
1054
1710





mays]




ACN31536
unknown [Zea mays]
0.78
1055
1711




NP_001142815
hypothetical protein
0.79
1056
1712





LOC100275194 [Zea mays]





> gi|195610158|gb|ACG26909.1|





hypothetical protein [Zea





mays]




NP_001148700
caleosin related protein [Zea
0.77
1057
1713





mays]





> gi|195621488|gb|ACG32574.1|





caleosin related protein





[Zea mays]



632-652
XP_002448765
hypothetical protein
1.00
1058
1714





SORBIDRAFT_06g032800





[Sorghum bicolor]





> gi|241939948|gb|EES13093.1|





hypothetical protein





SORBIDRAFT_06g032800





[Sorghum bicolor]




NP_001131374
hypothetical protein
0.99
1059
1715





LOC100192699 [Zea mays]





> gi|195620734|gb|ACG32197.1|





oligosaccharyl





transferase STT3 subunit [Zea





mays]




NP_001168720
hypothetical protein
0.98
1060
1716





LOC100382512 [Zea mays]





> gi|223950395|gb|ACN29281.1|





unknown [Zea mays]




NP_001054248
Os04g0675500 [Oryza sativa
0.96
1061
1717





Japonica Group]





> gi|38344929|emb|CAE03245.2|





OSJNBa0018M05.20





[Oryza sativa Japonica Group]





> gi|90399055|emb|CAJ86104.1|





H0103C06.8 [Oryza





sativa Indica Group]





> gi|113565819|dbj|BAF16162.1|





Os04g0675500 [Oryza





sativa Japonica Group]





> gi|125550210|gb|EAY96032.1|





hypothetical protein





OsI_17905 [Oryza sativa





Indica Group]





> gi|125592048|gb|EAZ32398.1|





hypothetical protein





OsJ_16609 [Oryza sativa





Japonica Group]





> gi|215708677|dbj|BAG93946.1|





unnamed protein





product [Oryza sativa Japonica





Group]




BAJ87792
predicted protein [Hordeum
0.95
1062
1718





vulgare subsp. vulgare]




CBG76274
OO_Ba0005L10-
0.93
1063
1719





OO_Ba0081K17.25 [Oryza





officinalis]




XP_002269119
PREDICTED: hypothetical
0.86
1064
1720





protein [Vitis vinifera]




XP_002318345
predicted protein [Populus
0.86
1065
1721





trichocarpa]





> gi|222859018|gb|EEE96565.1|





predicted protein





[Populus trichocarpa]




XP_002891078
hypothetical protein
0.85
1066
1722





ARALYDRAFT_891007





[Arabidopsis lyrata subsp.





lyrata]





> gi|297336920|gb|EFH67337.1|





hypothetical protein





ARALYDRAFT_891007





[Arabidopsis lyrata subsp.





lyrata]




XP_002329687
predicted protein [Populus
0.85
1067
1723





trichocarpa]





> gi|222870595|gb|EEF07726.1|





predicted protein [Populus





trichocarpa]



795-815
XP_002458747
hypothetical protein
1.00
1068
1724





SORBIDRAFT_03g039530





[Sorghum bicolor]





> gi|241930722|gb|EES03867.1|





hypothetical protein





SORBIDRAFT_03g039530





[Sorghum bicolor]




NP_001147942
L-ascorbate oxidase [Zea
0.96
1069
1725





mays]





> gi|195614732|gb|ACG29196.1|





L-ascorbate oxidase





precursor [Zea mays]




ACN34362
unknown [Zea mays]
0.96
1070
1726




EEC71780
hypothetical protein
0.86
1071





OsI_04394 [Oryza sativa





Indica Group]




NP_001044773
Os01g0842500 [Oryza sativa
0.86
1072
1727





Japonica Group]





> gi|19571025|dbj|BAB86452.1|





putative laccase LAC5-6





[Oryza sativa Japonica Group]





> gi|113534304|dbj|BAF06687.1|





Os01g0842500 [Oryza





sativa Japonica Group]





> gi|125572601|gb|EAZ14116.1|





hypothetical protein





OsJ_04040 [Oryza sativa





Japonica Group]





> gi|215694814|dbj|BAG90005.1|





unnamed protein





product [Oryza sativa Japonica





Group]




BAJ84890
predicted protein [Hordeum
0.84
1073
1728





vulgare subsp. vulgare]




BAJ96691
predicted protein [Hordeum
0.84
1074
1729





vulgare subsp. vulgare]




NP_001105875
putative laccase [Zea mays]
0.79
1075
1730





> gi|84618781|emb|CAJ30500.1|





putative laccase [Zea





mays]




ACN28855
unknown [Zea mays]
0.79
1076
1731




XP_002458746
hypothetical protein
0.78
1077
1732





SORBIDRAFT_03g039520





[Sorghum bicolor]





> gi|241930721|gb|EES03866.1|





hypothetical protein





SORBIDRAFT_03g039520





[Sorghum bicolor]



198-218
XP_002458746
hypothetical protein
1.00
1078
1733





SORBIDRAFT_03g039520





[Sorghum bicolor]





> gi|241930721|gb|EES03866.1|





hypothetical protein





SORBIDRAFT_03g039520





[Sorghum bicolor]




NP_001105875
putative laccase [Zea mays]
0.94
1079
1734





> gi|84618781|emb|CAJ30500.1|





putative laccase [Zea





mays]




NP_001146658
hypothetical protein
0.93
1080
1735





LOC100280258 [Zea mays]





> gi|219888209|gb|ACL54479.1|





unknown [Zea mays]




ACN28855
unknown [Zea mays]
0.94
1081
1736




NP_001044772
Os01g0842400 [Oryza sativa
0.80
1082
1737





Japonica Group]





> gi|75321217|sp|Q5N9X2.1|





LAC4_ORYSJ RecName:





Full = Laccase-4; AltName:





Full = Benzenediol:oxygen





oxidoreductase 4; AltName:





Full = Diphenol oxidase 4;





AltName: Full = Urishiol





oxidase 4; Flags: Precursor





> gi|56784239|dbj|BAD81734.1|





putative laccase LAC5-6





[Oryza sativa Japonica Group]





> gi|113534303|dbj|BAF06686.1|





Os01g0842400 [Oryza





sativa Japonica Group]





> gi|215697155|dbj|BAG91149.1|





unnamed protein





product [Oryza sativa Japonica





Group]




EAZ14115
hypothetical protein
0.80
1083





OsJ_04039 [Oryza sativa





Japonica Group]




EEC71777
hypothetical protein
0.80
1084





OsI_04389 [Oryza sativa





Indica Group]




BAJ99773
predicted protein [Hordeum
0.78
1085
1738





vulgare subsp. vulgare]




XP_002458747
hypothetical protein
0.78
1086
1739





SORBIDRAFT_03g039530





[Sorghum bicolor]





> gi|241930722|gb|EES03867.1|





hypothetical protein





SORBIDRAFT_03g039530





[Sorghum bicolor]




AAC04576
putative high-pI laccase
0.79
1087
1740





[Oryza sativa Japonica Group]



751-771
XP_002456566
hypothetical protein
1.00
1088
1741





SORBIDRAFT_03g038550





[Sorghum bicolor]





> gi|241928541|gb|EES01686.1|





hypothetical protein





SORBIDRAFT_03g038550





[Sorghum bicolor]




NP_001148479
L-ascorbate oxidase [Zea
0.92
1089
1742





mays]





> gi|195619672|gb|ACG31666.1|





L-ascorbate oxidase





precursor [Zea mays]




NP_001183899
hypothetical protein
0.90
1090
1743





LOC100502492 [Zea mays]





> gi|223948465|gb|ACN28316.1|





unknown [Zea mays]





> gi|238015342|gb|ACR38706.1|





unknown [Zea mays]




NP_001044679
Os01g0827300 [Oryza sativa
0.84
1091
1744





Japonica Group]





> gi|75331868|sp|Q941X2.1|





LAC3_ORYSJ RecName:





Full = Benzenediol: oxygen





oxidoreductase 3; AltName:





Full = Diphenol oxidase 3;





AltName: Full = Urishiol





oxidase 3; Flags: Precursor





> gi|15624045|dbj|BAB68098.1|





putative laccase [Oryza





sativa Japonica Group]





> gi|113534210|dbj|BAF06593.1|





Os01g0827300 [Oryza





sativa Japonica Group]





> gi|215701334|dbj|BAG92758.1|





unnamed protein





product [Oryza sativa Japonica





Group]





> gi|222619479|gb|EEE55611.1|





hypothetical protein





OsJ_03932 [Oryza sativa





Japonica Group]




ACN27868
unknown [Zea mays]
0.82
1092
1745




XP_002315131
laccase 90c [Populus
0.71
1093
1746





trichocarpa]





> gi|222864171|gb|EEF01302.1|





laccase 90c [Populus





trichocarpa]




ABK92474
unknown [Populus
0.70
1094
1747





trichocarpa]




XP_002312186
laccase 90a [Populus
0.70
1095
1748





trichocarpa]





> gi|222852006|gb|EEE89553.1|





laccase 90a [Populus





trichocarpa]



868-888
NP_001141205
hypothetical protein
1.00
1096
1749





LOC100273292 [Zea mays]





> gi|223944003|gb|ACN26085.1|





unknown [Zea mays]




ACF85713
unknown [Zea mays]
0.91
1097
1750




XP_002446013
hypothetical protein
0.84
1098
1751





SORBIDRAFT_06g000500





[Sorghum bicolor]





> gi|241937196|gb|EES10341.1|





hypothetical protein





SORBIDRAFT_06g000500





[Sorghum bicolor]




XP_002446014
hypothetical protein
0.81
1099
1752





SORBIDRAFT_06g000510





[Sorghum bicolor]





> gi|241937197|gb|EES10342.1|





hypothetical protein





SORBIDRAFT_06g000510





[Sorghum bicolor]




CAH67827
B0616E02-H0507E05.3
0.80
1100
1753





[Oryza sativa Indica Group]





> gi|125546940|gb|EAY92762.1|





hypothetical protein





OsI_14564 [Oryza sativa





Indica Group]





> gi|125589087|gb|EAZ29437.1|





hypothetical protein





OsJ_13511 [Oryza sativa





Japonica Group]




BAJ88131
predicted protein [Hordeum
0.83
1101
1754





vulgare subsp. vulgare]



172-192
NP_001148700
caleosin related protein [Zea
1.00
1102
1755





mays]





> gi|195621488|gb|ACG32574.1|





caleosin related protein





[Zea mays]




ACN31536
unknown [Zea mays]
0.99
1103
1756




ACG36841
caleosin related protein [Zea
0.83
1104
1757





mays]




NP_001142815
hypothetical protein
0.82
1105
1758





LOC100275194 [Zea mays]





> gi|195610158|gb|ACG26909.1|





hypothetical protein [Zea





mays]




XP_002454327
hypothetical protein
0.76
1106
1759





SORBIDRAFT_04g028730





[Sorghum bicolor]





> gi|241934158|gb|EES07303.1|





hypothetical protein





SORBIDRAFT_04g028730





[Sorghum bicolor]



782-802
XP_002465485
hypothetical protein
1.00
1107
1760





SORBIDRAFT_01g039690





[Sorghum bicolor]





> gi|241919339|gb|EER92483.1|





hypothetical protein





SORBIDRAFT_01g039690





[Sorghum bicolor]




NP_001131665
hypothetical protein
0.92
1108
1761





LOC100193025 [Zea mays]





> gi|194692196|gb|ACF80182.1|





unknown [Zea mays]




NP_001049699
Os03g0273200 [Oryza sativa
0.82
1109
1762





Japonica Group]





> gi|122247226|sp|Q10ND7.1|





LAC10_ORYSJ RecName:





Full = Laccase-10; AltName:





Full = Benzenediol:oxygen





oxidoreductase 10; AltName:





Full = Diphenol oxidase 10;





AltName: Full = Urishiol





oxidase 10; Flags: Precursor





> gi|108707435|gb|ABF95230.1|





laccase, putative,





expressed [Oryza sativa





Japonica Group]





> gi|113548170|dbj|BAF11613.1|





Os03g0273200 [Oryza





sativa Japonica Group]





> gi|215704111|dbj|BAG92951.1|





unnamed protein





product [Oryza sativa Japonica





Group]





> gi|215765805|dbj|BAG87502.1|





unnamed protein





product [Oryza sativa Japonica





Group]





> gi|222624652|gb|EEE58784.1|





hypothetical protein





OsJ_10313 [Oryza sativa





Japonica Group]




XP_002531824
laccase, putative [Ricinus
0.73
1110
1763





communis]





> gi|223528520|gb|EEF30544.1|





laccase, putative [Ricinus





communis]




XP_002278215
PREDICTED: hypothetical
0.73
1111
1764





protein [Vitis vinifera]




CBI16199
unnamed protein product
0.72
1112





[Vitis vinifera]




XP_002278232
PREDICTED: hypothetical
0.73
1113
1765





protein [Vitis vinifera]




XP_002282823
PREDICTED: hypothetical
0.74
1114
1766





protein [Vitis vinifera]



943-963
NP_001183899
hypothetical protein
1.00
1115
1767





LOC100502492 [Zea mays]





> gi|223948465|gb|ACN28316.1|





unknown [Zea mays]





> gi|238015342|gb|ACR38706.1|





unknown [Zea mays]




NP_001148479
L-ascorbate oxidase [Zea
0.90
1116
1768





mays]





> gi|195619672|gb|ACG31666.1|





L-ascorbate oxidase





precursor [Zea mays]




XP_002456566
hypothetical protein
0.89
1117
1769





SORBIDRAFT_03g038550





[Sorghum bicolor]





> gi|241928541|gb|EES01686.1|





hypothetical protein





SORBIDRAFT_03g038550





[Sorghum bicolor]




NP_001044679
Os01g0827300 [Oryza sativa
0.83
1118
1770





Japonica Group]





> gi|75331868|sp|Q941X2.1|





LAC3_ORYSJ RecName:





Full = Laccase-3; AltName:





Full = Benzenediol:oxygen





oxidoreductase 3; AltName:





Full = Diphenol oxidase 3;





AltName: Full = Urishiol





oxidase 3; Flags: Precursor





> gi|15624045|dbj|BAB68098.1|





putative laccase [Oryza





sativa Japonica Group]





> gi|113534210|dbj|BAF06593.1|





Os01g0827300 [Oryza





sativa Japonica Group]





> gi|215701334|dbj|BAG92758.1|





unnamed protein





product [Oryza sativa Japonica





Group]





> gi|222619479|gb|EEE55611.1|





hypothetical protein





OsJ_03932 [Oryza sativa





Japonica Group]




ACN27868
unknown [Zea mays]
0.80
1119
1771




XP_002315131
laccase 90c [Populus
0.70
1120
1772





trichocarpa]





> gi|222864171|gb|EEF01302.1|





laccase 90c [Populus





trichocarpa]




CBI25418
unnamed protein product
0.71
1121
1773





[Vitis vinifera]




XP_002273875
PREDICTED: hypothetical
0.71
1122
1774





protein [Vitis vinifera]



852-872
NP_001105875
putative laccase [Zea mays]
1.00
1123
1775





> gi|84618781|emb|CAJ30500.1|





putative laccase [Zea





mays]




ACN28855
unknown [Zea mays]
0.98
1124
1776




NP_001146658
hypothetical protein
0.94
1125
1777





LOC100280258 [Zea mays]





> gi|219888209|gb|ACL54479.1|





unknown [Zea mays]




XP_002458746
hypothetical protein
0.91
1126
1778





SORBIDRAFT_03g039520





[Sorghum bicolor]





> gi|241930721|gb|EES03866.1|





hypothetical protein





SORBIDRAFT_03g039520





[Sorghum bicolor]




NP_001044772
Os01g0842400 [Oryza sativa
0.80
1127
1779





Japonica Group]





> gi|75321217|sp|Q5N9X2.1|





LAC4_ORYSJ RecName:





Full = Laccase-4; AltName:





Full = Benzenediol:oxygen





oxidoreductase 4; AltName:





Full = Diphenol oxidase 4;





AltName: Full = Urishiol





oxidase 4; Flags: Precursor





> gi|56784239|dbj|BAD81734.1|





putative laccase LAC5-6





[Oryza sativa Japonica Group]





> gi|113534303|dbj|BAF06686.1|





Os01g0842400 [Oryza





sativa Japonica Group]





> gi|215697155|dbj|BAG91149.1|





unnamed protein





product [Oryza sativa Japonica





Group]




EAZ14115
hypothetical protein
0.80
1128





OsJ_04039 [Oryza sativa





Japonica Group]




EEC71777
hypothetical protein
0.80
1129





OsI_04389 [Oryza sativa





Indica Group]




AAC04576
putative high-pI laccase
0.79
1130
1780





[Oryza sativa Japonica Group]




BAJ99773
predicted protein [Hordeum
0.77
1131
1781





vulgare subsp. vulgare]




XP_002458747
hypothetical protein
0.78
1132
1782





SORBIDRAFT_03g039530





[Sorghum bicolor]





> gi|241930722|gb|EES03867.1|





hypothetical protein





SORBIDRAFT_03g039530





[Sorghum bicolor]



801-821
XP_002441216
hypothetical protein
1.00
1133
1783





SORBIDRAFT_09g022460





[Sorghum bicolor]





> gi|241946501|gb|EES19646.1|





hypothetical protein





SORBIDRAFT_09g022460





[Sorghum bicolor]




NP_001105874
putative laccase [Zea mays]
0.89
1134
1784





> gi|84618777|emb|CAJ30498.1|





putative laccase [Zea





mays]




Q0DHL5
RecName: Full = Putative
0.88
1135





laccase-11; AltName:





Full = Benzenediol:oxygen





oxidoreductase 11; AltName:





Full = Diphenol ocidase 11;





AltName: Full = Urishiol





oxidase 11





> gi|222631843|gb|EEE63975.1|





hypothetical protein





OsJ_18801 [Oryza sativa





Japonica Group]




NP_001055744
Os05g0458300 [Oryza sativa
0.81
1136
1785





Japonica Group]





> gi|113579295|dbj|BAF17658.1|





Os05g0458300 [Oryza





sativa Japonica Group]




XP_002456622
hypothetical protein
0.76
1137
1786





SORBIDRAFT_03g039570





[Sorghum bicolor]





> gi|241928597|gb|EES01742.1|





hypothetical protein





SORBIDRAFT_03g039570





[Sorghum bicolor]




ACG47007
L-ascorbate oxidase precursor
0.76
1138
1787





[Zea mays]




NP_001105921
putative laccase [Zea mays]
0.74
1139
1788





> gi|84618783|emb|CAJ30497.1|





putative laccase [Zea





mays]



673-693
NP_001168720
hypothetical protein
1.00
1140
1789





LOC100382512 [Zea mays]





> gi|223950395|gb|ACN29281.1|





unknown [Zea mays]




XP_002448765
hypothetical protein
0.98
1141
1790





SORBIDRAFT_06g032800





[Sorghum bicolor]





> gi|241939948|gb|EES13093.1|





hypothetical protein





SORBIDRAFT_06g032800





[Sorghum bicolor]




NP_001131374
hypothetical protein
0.98
1142
1791





LOC100192699 [Zea mays]





> gi|195620734|gb|ACG32197.1|





oligosaccharyl





transferase STT3 subunit [Zea





mays]




NP_001054248
Os04g0675500 [Oryza sativa
0.96
1143
1792





Japonica Group]





> gi|38344929|emb|CAE03245.2|





OSJNBa0018M05.20





[Oryza sativa Japonica Group]





> gi|90399055|emb|CAJ86104.1|





H0103C06.8 [Oryza





sativa Indica Group]





> gi|113565819|dbj|BAF16162.1|





Os04g0675500 [Oryza





sativa Japonica Group]





> gi|125550210|gb|EAY96032.1|





hypothetical protein





OsI_17905 [Oryza sativa





Indica Group]





> gi|125592048|gb|EAZ32398.1|





hypothetical protein





OsJ_16609 [Oryza sativa





Japonica Group]





> gi|215708677|dbj|BAG93946.1|





unnamed protein





product [Oryza sativa Japonica





Group]




BAJ87792
predicted protein [Hordeum
0.94
1144
1793





vulgare subsp. vulgare]




XP_002269119
PREDICTED: hypothetical
0.86
1145
1794





protein [Vitis vinifera]




CBG76274
OO_Ba0005L10-
0.93
1146
1795





OO_Ba0081K17.25 [Oryza





officinalis]




XP_002318345
predicted protein [Populus
0.86
1147
1796





trichocarpa]





> gi|222859018|gb|EEE96565.1|





predicted protein





[Populus trichocarpa]




XP_002891078
hypothetical protein
0.86
1148
1797





ARALYDRAFT_891007





[Arabidopsis lyrata subsp.





lyrata]





> gi|297336920|gb|EFH67337.1|





hypothetical protein





ARALYDRAFT_891007





[Arabidopsis lyrata subsp.





lyrata]




XP_002329687
predicted protein [Populus
0.85
1149
1798





trichocarpa]





> gi|222870595|gb|EEF07726.1|





predicted protein [Populus





trichocarpa]



1539-1559
XP_002462694
hypothetical protein
1.00
1150
1799





SORBIDRAFT_02g030360





[Sorghum bicolor]





> gi|241926071|gb|EER99215.1|





hypothetical protein





SORBIDRAFT_02g030360





[Sorghum bicolor]




NP_001168360
hypothetical protein
0.92
1151
1800





LOC100382128 [Zea mays]





> gi|223947749|gb|ACN27958.1|





unknown [Zea mays]




NP_001063714
Os09g0524300 [Oryza sativa
0.80
1152
1801





Japonica Group]





> gi|52076031|dbj|BAD46484.1|





ethionine resistance





protein-like [Oryza sativa





Japonica Group]





> gi|255679074|dbj|BAF25628.2|





Os09g0524300 [Oryza





sativa Japonica Group]




ABG73448
MATE efflux family protein
0.80
1153
1802





[Oryza brachyantha]




EAZ09807
hypothetical protein
0.80
1154





OsI_32095 [Oryza sativa





Indica Group]




XP_002444635
hypothetical protein
0.73
1155
1803





SORBIDRAFT_07g025190





[Sorghum bicolor]





> gi|241940985|gb|EES14130.1|





hypothetical protein





SORBIDRAFT_07g025190





[Sorghum bicolor]




BAD09230
putative ripening regulated
0.70
1156
1804





protein DDTFR18 [Oryza





sativa Japonica Group]



1198-1218
NP_001146658
hypothetical protein
1.00
1157
1805





LOC100280258 [Zea mays]





> gi|219888209|gb|ACL54479.1|





unknown [Zea mays]




NP_001105875
putative laccase [Zea mays]
0.95
1158
1806





> gi|84618781|emb|CAJ30500.1|





putative laccase [Zea





mays]




ACN28855
unknown [Zea mays]
0.92
1159
1807




XP_002458746
hypothetical protein
0.91
1160
1808





SORBIDRAFT_03g039520





[Sorghum bicolor]





> gi|241930721|gb|EES03866.1|





hypothetical protein





SORBIDRAFT_03g039520





[Sorghum bicolor]




NP_001044772
Os01g0842400 [Oryza sativa
0.80
1161
1809





Japonica Group]





> gi|75321217|sp|Q5N9X2.1|





LAC4_ORYSJ RecName:





Full = Laccase-4; AltName:





Full = Benzenediol:oxygen





oxidoreductase 4; AltName:





Full = Diphenol oxidase 4;





AltName: Full = Urishiol





oxidase 4; Flags: Precursor





> gi|56784239|dbj|BAD81734.1|





putative laccase LAC5-6





[Oryza sativa Japonica Group]





> gi|113534303|dbj|BAF06686.1|





Os01g0842400 [Oryza





sativa Japonica Group]





> gi|215697155|dbj|BAG91149.1|





unnamed protein





product [Oryza sativa Japonica





Group]




EAZ14115
hypothetical protein
0.80
1162





OsJ_04039 [Oryza sativa





Japonica Group]




EEC71777
hypothetical protein
0.80
1163





OsI_04389 [Oryza sativa





Indica Group]




BAJ99773
predicted protein [Hordeum
0.78
1164
1810





vulgare subsp. vulgare]




AAC04576
putative high-pI laccase
0.78
1165
1811





[Oryza sativa Japonica Group]




XP_002458747
hypothetical protein
0.77
1166
1812





SORBIDRAFT_03g039530





[Sorghum bicolor]





> gi|241930722|gb|EES03867.1|





hypothetical protein





SORBIDRAFT_03g039530





[Sorghum bicolor]



934-954
ACN27868
unknown [Zea mays]
1.00
1167
1813




NP_001148479
L-ascorbate oxidase [Zea
0.94
1168
1814





mays]





> gi|195619672|gb|ACG31666.1|





L-ascorbate oxidase





precursor [Zea mays]




XP_002456566
hypothetical protein
0.90
1169
1815





SORBIDRAFT_03g038550





[Sorghum bicolor]





> gi|241928541|gb|EES01686.1|





hypothetical protein





SORBIDRAFT_03g038550





[Sorghum bicolor]




NP_001183899
hypothetical protein
0.88
1170
1816





LOC100502492 [Zea mays]





> gi|223948465|gb|ACN28316.1|





unknown [Zea mays]





> gi|238015342|gb|ACR38706.1|





unknown [Zea mays]




NP_001044679
Os01g0827300 [Oryza sativa
0.81
1171
1817





Japonica Group]





> gi|75331868|sp|Q941X2.1|





LAC3_ORYSJ RecName:





Full = Laccase-3; AltName:





Full = Benzenediol:oxygen





oxidoreductase 3; AltName:





Full = Diphenol oxidase 3;





AltName: Full = Urishiol





oxidase 3; Flags: Precursor





> gi|15624045|dbj|BAB68098.1|





putative laccase [Oryza





sativa Japonica Group]





> gi|113534210|dbj|BAF06593.1|





Os01g0827300 [Oryza





sativa Japonica Group]





> gi|215701334|dbj|BAG92758.1|





unnamed protein





product [Oryza sativa Japonica





Group]





> gi|222619479|gb|EEE55611.1|





hypothetical protein





OsJ_03932 [Oryza sativa





Japonica Group]


Predicted
1620-1643
NP_001137083
hypothetical protein
1.00
1172
1818


folded


LOC100217256 [Zea mays]


24-nts-


> gi|194698278|gb|ACF83223.1|


long seq


unknown [Zea mays]


52739




XP_002446946
hypothetical protein
0.75
1173
1819





SORBIDRAFT_06g025600





[Sorghum bicolor]





> gi|241938129|gb|EES11274.1|





hypothetical protein





SORBIDRAFT_06g025600





[Sorghum bicolor]


Predicted
334-354
XP_002459032
hypothetical protein
1.00
1174
1820


zma


SORBIDRAFT_03g044830


mir


[Sorghum bicolor]


49816


> gi|241931007|gb|EES04152.1|





hypothetical protein





SORBIDRAFT_03g044830





[Sorghum bicolor]




NP_001131974
hypothetical protein
0.90
1175
1821





LOC100193372 [Zea mays]





> gi|194693076|gb|ACF80622.1|





unknown [Zea mays]




BAJ85237
predicted protein [Hordeum
0.84
1176
1822





vulgare subsp. vulgare]





> gi|326511587|dbj|BAJ91938.1|





predicted protein





[Hordeum vulgare subsp.





vulgare]




NP_001045294
Os01g0931100 [Oryza sativa
0.85
1177
1823





Japonica Group]





> gi|57899654|dbj|BAD87323.1|





unknown protein [Oryza





sativa Japonica Group]





> gi|57900117|dbj|BAD88179.1|





unknown protein [Oryza





sativa Japonica Group]





> gi|113534825|dbj|BAF07208.1|





Os01g0931100 [Oryza





sativa Japonica Group]





> gi|215697092|dbj|BAG91086.1|





unnamed protein





product [Oryza sativa Japonica





Group]



116-136
CAE04743
OSJNBb0060E08.6 [Oryza
1.00
1178
1824





sativa Japonica Group]





> gi|116309791|emb|CAH66831.1|





OSIGBa0148A10.8





[Oryza sativa Indica Group]




NP_001053941
0s04g0625800 [Oryza sativa
1.00
1179
1825





Japonica Group]





> gi|113565512|dbj|BAF15855.1|





Os04g0625800 [Oryza





sativa Japonica Group]





> gi|215767966|dbj|BAH00195.1|





unnamed protein





product [Oryza sativa Japonica





Group]




EAY95645
hypothetical protein
1.00
1180





OsI_17510 [Oryza sativa





Indica Group]




NP_001131852
hypothetical protein
0.79
1181
1826





LOC100193230 [Zea mays]





> gi|194692726|gb|ACF80447.1|





unknown [Zea mays]




BAK03410
predicted protein [Hordeum
0.81
1182
1827





vulgare subsp. vulgare]





> gi|326525779|dbj|BAJ88936.1|





predicted protein





[Hordeum vulgare subsp.





vulgare]




XP_002447134
hypothetical protein
0.78
1183
1828





SORBIDRAFT_06g029180





[Sorghum bicolor]





> gi|241938317|gb|EES11462.1|





hypothetical protein





SORBIDRAFT_06g029180





[Sorghum bicolor]




NP_001168401
hypothetical protein
0.77
1184
1829





LOC100382170 [Zea mays]





> gi|223948043|gb|ACN28105.1|





unknown [Zea mays]




ACF85557
unknown [Zea mays]
0.74
1185
1830




NP_001141359
hypothetical protein
0.74
1186
1831





LOC100273450 [Zea mays]





> gi|194704160|gb|ACF86164.1|





unknown [Zea mays]



 81-101
XP_002463519
hypothetical protein
1.00
1187
1832





SORBIDRAFT_01g001270





[Sorghum bicolor]





> gi|241917373|gb|EER90517.1|





hypothetical protein





SORBIDRAFT_01g001270





[Sorghum bicolor]




BAK01513
predicted protein [Hordeum
0.86
1188
1833





vulgare subsp. vulgare]




NP_001051898
Os03g0849600 [Oryza sativa
0.85
1189
1834





Japonica Group]





> gi|28269403|gb|AAO37946.1|





putative conserved





oligomeric Golgi complex





component [Oryza sativa





Japonica Group]





> gi|108712114|gb|ABF99909.1|





brefeldin A-sensitive





Golgi protein, putative,





expressed [Oryza sativa





Japonica Group]





> gi|113550369|dbj|BAF13812.1|





Os03g0849600 [Oryza





sativa Japonica Group]





> gi|125588645|gb|EAZ29309.1|





hypothetical protein





OsJ_13370 [Oryza sativa





Japonica Group]




EEC76534
hypothetical protein
0.85
1190





OsI_14326 [Oryza sativa





Indica Group]



322-342
NP_001168448
hypothetical protein
1.00
1191
1835





LOC100382221 [Zea mays]





> gi|223948365|gb|ACN28266.1|





unknown [Zea mays]



 98-118
BAD05744
putative RNA Binding Protein
1.00
1192
1836





45 [Oryza sativa Japonica





Group]





> gi|40253847|dbj|BAD05783.1|





putative RNA Binding





Protein 45 [Oryza sativa





Japonica Group]




EEE68172
hypothetical protein
0.75
1193





OSJ_26296 [Oryza sativa





Japonica Group]




ACF85557
unknown [Zea mays]
0.78
1194
1837




NP_001141359
hypothetical protein
0.78
1195
1838





LOC100273450 [Zea mays]





> gi|194704160|gb|ACF86164.1|





unknown [Zea mays]


aqc-
955-975
AAX83875
teosinte glume architecture 1
1.00
1196
1839


miR529


[Zea mays subsp. mays]




AAX83872
teosinte glume architecture 1
0.99
1197
1840





[Zea mays subsp. mays]




AAX83873
teosinte glume architecture 1
0.99
1198
1841





[Zea mays subsp. mays]





> gi|62467440|gb|AAX83874.1|





teosinte glume





architecture 1 [Zea mays





subsp. mays]




XP_002445815
hypothetical protein
0.80
1199
1842





SORBIDRAFT_07g026220





[Sorghum bicolor]





> gi|241942165|gb|EES15310.1|





hypothetical protein





SORBIDRAFT_07g026220





[Sorghum bicolor]



831-851
ADX60108
SBP transcription factor [Zea
1.00
1200
1843





mays]




XP_002446904
hypothetical protein
0.72
1201
1844





SORBIDRAFT_06g024630





[Sorghum bicolor]





> gi|241938087|gb|EES11232.1|





hypothetical protein





SORBIDRAFT_06g024630





[Sorghum bicolor]



882-902
ACN30570
unknown [Zea mays]
1.00
1202
1845





> gi|223973927|gb|ACN31151.1|





unknown [Zea mays]





> gi|323388595|gb|ADX60102.1|





SBP transcription factor





[Zea mays]




NP_001145445
hypothetical protein
0.98
1203
1846





LOC100278824 [Zea mays]





> gi|195656399|gb|ACG47667.1|





hypothetical protein [Zea





mays]




XP_002450775
hypothetical protein
0.87
1204
1847





SORBIDRAFT_05g017510





[Sorghum bicolor]





> gi|241936618|gb|EES09763.1|





hypothetical protein





SORBIDRAFT_05g017510





[Sorghum bicolor]



656-676
NP_001137049
hypothetical protein
1.00
1205
1848





LOC100217221 [Zea mays]





> gi|194698154|gb|ACF83161.1|





unknown [Zea mays]



1248-1268
XP_002447211
hypothetical protein
1.00
1206
1849





SORBIDRAFT_06g030520





[Sorghum bicolor]





> gi|241938394|gb|EES11539.1|





hypothetical protein





SORBIDRAFT_06g030520





[Sorghum bicolor]




NP_001132831
hypothetical protein
0.90
1207
1850





LOC100194321 [Zea mays]





> gi|194695516|gb|ACF81842.1|





unknown [Zea mays]




NP_001054060
Os04g0644700 [Oryza sativa
0.80
1208
1851





Japonica Group]





> gi|148886836|sp|P0C541.1|





COPE2_ORYSJ RecName:





Full = Coatomer subunit





epsilon-2; AltName:





Full = Epsilon-coat protein 2;





Short = Epsilon-COP 2





> gi|38344895|emb|CAD41918.2|





OSJNBa0033G05.19





[Oryza sativa Japonica Group]





> gi|113565631|dbj|BAF15974.1|





Os04g0644700 [Oryza





sativa Japonica Group]





> gi|125591839|gb|EAZ32189.1|





hypothetical protein





OsJ_16395 [Oryza sativa





Japonica Group]




A2XY73
RecName: Full = Coatomer
0.80
1209





subunit epsilon-2; AltName:





Full = Epsilon-coat protein 2;





Short = Epsilon-COP 2





> gi|90399097|emb|CAJ86157.1|





H0413E07.10 [Oryza





sativa Indica Group]




EAY95783
hypothetical protein
0.80
1210





OsI_17658 [Oryza sativa





Indica Group]



 82-102
ACR34442
unknown [Zea mays]
1.00
1211
1852



 82-102
NP_00145733
hypothetical protein
1.00
1212
1853





LOC100279240 [Zea mays]





> gi|219884215|gb|ACL52482.1|





unknown [Zea mays]




XP_002460490
hypothetical protein
0.87
1213
1854





SORBIDRAFT_02g029300





[Sorghum bicolor]





> gi|241923867|gb|EER97011.1|





hypothetical protein





SORBIDRAFT_02g029300





[Sorghum bicolor]




NP_001063612
Os09g0507100 [Oryza sativa
0.71
1214
1855





Japonica Group]





> gi|122234416|sp|Q0JOK1.1|





SPL18_ORYSJ RecName:





Full = Squamosa promoter-





binding-like protein 18





> gi|113631845|dbj|BAF25526.1|





Os09g0507100 [Oryza





sativa Japonica Group]



 996-1016
ACF86782
unknown [Zea mays]
1.00
1215
1856





> gi|323388573|gb|ADX60091.1|





SBP transcription factor





[Zea mays]




ACG45113
squamosa promoter-binding-
1.00
1216
1857





like protein 9 [Zea mays]




XP_002462571
hypothetical protein
0.83
1217
1858





SORBIDRAFT_02g028420





[Sorghum bicolor]





> gi|241925948|gb|EER99092.1|





hypothetical protein





SORBIDRAFT_02g028420





[Sorghum bicolor]




NP_001136945
hypothetical protein
0.76
1218
1859





LOC100217104 [Zea mays]





> gi|194697718|gb|ACF82943.1|





unknown [Zea mays]



1120-1140
ACL52941
unknown [Zea mays]
1.00
1219
1860




XP_002444771
hypothetical protein
0.76
1220
1861





SORBIDRAFT_07g027740





[Sorghum bicolor]





> gi|241941121|gb|EES14266.1|





hypothetical protein





SORBIDRAFT_07g027740





[Sorghum bicolor]



1348-1368
XP_002438971
hypothetical protein
1.00
1221
1862





SORBIDRAFT_10g029190





[Sorghum bicolor]





> gi|241917194|gb|EER90338.1|





hypothetical protein





SORBIDRAFT_10g029190





[Sorghum bicolor]




NP_001149534
squamosa promoter-binding-
0.88
1222
1863





like protein 11 [Zea mays]





> gi|195627850|gb|ACG35755.1|





squamosa promoter-





binding-like protein 11 [Zea





mays]





> gi|195644948|gb|ACG41942.1|





squamosa promoter-





binding-like protein 11 [Zea





mays]



221-241
XP_002447219
hypothetical protein
1.00
1223
1864





SORBIDRAFT_06g030650





[Sorghum bicolor]





> gi|241938402|gb|EES11547.1|





hypothetical protein





SORBIDRAFT_06g030650





[Sorghum bicolor]




NP_001169344
hypothetical protein
0.92
1224
1865





LOC100383211 [Zea mays]





> gi|224028835|gb|ACN33493.1|





unknown [Zea mays]




NP_001147890
LOC100281500 [Zea mays]
0.90
1225
1866





> gi|195614420|gb|ACG29040.1|





zinc finger, C3HC4 type





family protein [Zea mays]




CAD41707
OSJNBa0010D21.9 [Oryza
0.74
1226
1867





sativa Japonica Group]





> gi|125549990|gb|EAY95812.1|





hypothetical protein





OsI_17683 [Oryza sativa





Indica Group]





> gi|125591860|gb|EAZ32210.1|





hypothetical protein





OsJ_16417 [Oryza sativa





Japonica Group]





> gi|215701084|dbj|BAG92508.1|





unnamed protein





product [Oryza sativa Japonica





Group]



973-993
NP_001136945
hypothetical protein
1.00
1227
1868





LOC100217104 [Zea mays]





> gi|194697718|gb|ACF82943.1|





unknown [Zea mays]




XP_002462571
hypothetical protein
0.82
1228
1869





SORBIDRAFT_02g028420





[Sorghum bicolor]





> gi|241925948|gb|EER99092.1|





hypothetical protein





SORBIDRAFT_02g028420





[Sorghum bicolor]




ACF86782
unknown [Zea mays]
0.76
1229
1870





> gi|323388573|gb|ADX60091.1|





SBP transcription factor





[Zea mays]




ACG45113
squamosa promoter-binding-
0.76
1230
1871





like protein 9 [Zea mays]



558-578
CAB56631
SBP-domain protein 5 [Zea
1.00
1231
1872





mays]




XP_002444771
hypothetical protein
0.85
1232
1873





SORBIDRAFT_07g027740





[Sorghum bicolor]





> gi|241941121|gb|EES14266.1|





hypothetical protein





SORBIDRAFT_07g027740





[Sorghum bicolor]




ACL52941
unknown [Zea mays]
0.78
1233
1874



1410-1430
NP_001152658
MTA/SAH nucleosidase [Zea
1.00
1234
1875





mays]





> gi|195658647|gb|ACG48791.1|





MTA/SAH nucleosidase





[Zea mays]





> gi|223973627|gb|ACN31001.1|





unknown [Zea mays]




ACF83838
unknown [Zea mays]
0.88
1235
1876




ACG39594
MTA/SAH nucleosidase [Zea
0.88
1236
1877





mays]




XP_002445813
hypothetical protein
0.88
1237
1878





SORBIDRAFT_07g026190





[Sorghum bicolor]





> gi|241942163|gb|EES15308.1|





hypothetical protein





SORBIDRAFT_07g026190





[Sorghum bicolor]




ACN31483
unknown [Zea mays]
0.90
1238
1879




NP_001056592
Os06g0112200 [Oryza sativa
0.80
1239
1880





Japonica Group]





> gi|7363290|dbj|BAA93034.1|





methylthioadenosine/S-





adenosyl homocysteine





nucleosidase [Oryza sativa





Japonica Group]





> gi|32352128|dbj|BAC78557.1|





hypothetical protein





[Oryza sativa Japonica Group]





> gi|113594632|dbj|BAF18506.1|





Os06g0112200 [Oryza





sativa Japonica Group]





> gi|125595804|gb|EAZ35584.1|





hypothetical protein





OsJ_19870 [Oryza sativa





Japonica Group]





> gi|215694661|dbj|BAG89852.1|





unnamed protein





product [Oryza sativa Japonica





Group]





> gi|215740802|dbj|BAG96958.1|





unnamed protein





product [Oryza sativa Japonica





Group]




AAL58883
methylthioadenosine/S-
0.79
1240
1881





adenosyl homocysteine





nucleosidase [Oryza sativa]




ABR25495
mta/sah nucleosidase [Oryza
0.79
1241
1882





sativa Indica Group]




BAK03317
predicted protein [Hordeum
0.78
1242
1883





vulgare subsp. vulgare]





> gi|326534118|dbj|BAJ89409.1|





predicted protein





[Hordeum vulgare subsp.





vulgare]




EAY99382
hypothetical protein
0.78
1243





OsI_21350 [Oryza sativa





Indica Group]



1197-1217
AAX83872
teosinte glume architecture 1
1.00
1244
1884





[Zea mays subsp. mays]




AAX83875
teosinte glume architecture 1
0.98
1245
1885





[Zea mays subsp. mays]




AAX83873
teosinte glume architecture 1
0.99
1246
1886





[Zea mays subsp. mays]





> gi|62467440|gb|AAX83874.1|





teosinte glume





architecture 1 [Zea mays





subsp. mays]




XP_002445815
hypothetical protein
0.80
1247
1887





SORBIDRAFT_07g026220





[Sorghum bicolor]





> gi|241942165|gb|EES15310.1|





hypothetical protein





SORBIDRAFT_07g026220





[Sorghum bicolor]




BAK05794
predicted protein [Hordeum
0.63
1248
1888





vulgare subsp. vulgare]


Predicted
748-771
NP_001143705
hypothetical protein
1.00
1281
1911


folded


LOC100276442 [Zea mays]


24-nts-


> gi|195625088|gb|ACG34374.1|


long seq


hypothetical protein [Zea


52792


mays]




ACF83056
unknown [Zea mays]
0.95
1282
1912



1078-1101
ACF83056
unknown [Zea mays]
1.00
1283
1913




NP_001143705
hypothetical protein
0.88
1284
1914





LOC100276442 [Zea mays]





> gi|195625088|gb|ACG34374.1|





hypothetical protein [Zea





mays]


Predicted
278-301
XP_002458944
hypothetical protein
1.00
1285
1915


folded


SORBIDRAFT_03g043140


24-nts-


[Sorghum bicolor]


long seq


> gi|241930919|gb|EES04064.1|


51757


hypothetical protein





SORBIDRAFT_03g043140





[Sorghum bicolor]




ACF79162
unknown [Zea mays]
0.98
1286
1916





> gi|194703646|gb|ACF85907.1|





unknown [Zea mays]




NP_001105336
fructose-bisphosphate
0.98
1287
1917





aldolase, cytoplasmic isozyme





[Zea mays]





> gi|113621|sp|P08440.1|ALF_MAIZE





RecName:





Full = Fructose-bisphosphate





aldolase, cytoplasmic isozyme





> gi|168420|gb|AAA33435.1|





aldolase [Zea mays]





> gi|295850|emb|CAA31366.1|





fructose bisphosphate





aldolase [Zea mays]





> gi|225624|prf||1307278A





cytoplasmic aldolase




NP_001150049
fructose-bisphosphate aldolase
0.98
1288
1918





cytoplasmic isozyme [Zea





mays]





> gi|194704898|gb|ACF86533.1|





unknown [Zea mays]





> gi|195636310|gb|ACG37623.1|





fructose-bisphosphate





aldolase cytoplasmic isozyme





[Zea mays]




XP_002453822
hypothetical protein
0.95
1289
1919





SORBIDRAFT_04g019020





[Sorghum bicolor]





> gi|241933653|gb|EES06798.1|





hypothetical protein





SORBIDRAFT_04g019020





[Sorghum bicolor]




P17784
RecName: Full = Fructose-
0.94
1290





bisphosphate aldolase





cytoplasmic isozyme;





AltName: Full = Gravity-





specific protein GSC 233





> gi|41398198|gb|AAS05825.1|





fructose 1,6-bisphosphate





aldolase [Oryza sativa





Japonica Group]





> gi|50878379|gb|AAT85154.1|





putative fructose-





bisphosphate aldolase [Oryza





sativa Japonica Group]





> gi|50878433|gb|AAT85207.1|





putative fructose-





bisphosphate aldolase [Oryza





sativa Japonica Group]





> gi|169244417|gb|ACA50482.1|





fructose-bisphosphate





aldolase [Oryza sativa





Japonica Group]





> gi|306415953|gb|ADM86851.1|





fructose-bisphosphate





aldolase [Oryza sativa





Japonica Group]




BAI59774
aldolase C-1 [Phyllostachys
0.94
1291
1920





edulis]




EAY94426
hypothetical protein
0.93
1292





OsI_16195 [Oryza sativa





Indica Group]




CAA37290
unnamed protein product
0.93
1293
1921





[Oryza sativa Japonica Group]




ABG65931
Fructose-bisphosphate
0.92
1294
1922





aldolase, cytoplasmic isozyme,





putative, expressed [Oryza





sativa Japonica Group]





> gi|125574121|gb|EAZ15405.1|





hypothetical protein





OsJ_30817 [Oryza sativa





Japonica Group]





> gi|215701125|dbj|BAG92549.1|





unnamed protein





product [Oryza sativa Japonica





Group]





> gi|215708712|dbj|BAG93981.1|





unnamed protein





product [Oryza sativa Japonica





Group]



686-709
NP_001145778
hypothetical protein
1.00
1295
1923





LOC100279285 [Zea mays]





> gi|219884395|gb|ACL52572.1|





unknown [Zea mays]




ACF87373
unknown [Zea mays]
1.00
1296
1924




ACG25535
ribosomal RNA apurinic site
0.99
1297
1925





specific lyase [Zea mays]




XP_002455890
hypothetical protein
0.79
1298
1926





SORBIDRAFT_03g026880





[Sorghum bicolor]





> gi|241927865|gb|EES01010.1|





hypothetical protein





SORBIDRAFT_03g026880





[Sorghum bicolor]



365-388
ACR36335
unknown [Zea mays]
1.00
1299
1927




ACR35742
unknown [Zea mays]
0.75
1300
1928



512-535
XP_002458102
hypothetical protein
1.00
1301
1929





SORBIDRAFT_03g026990





[Sorghum bicolor]





> gi|241930077|gb|EE503222.1|





hypothetical protein





SORBIDRAFT_03g026990





[Sorghum bicolor]




NP_001151540
hydrogen-transporting ATP
0.93
1302
1930





synthase, rotational





mechanism [Zea mays]





> gi|195605790|gb|ACG24725.1|





hydrogen-transporting





ATP synthase, rotational





mechanism [Zea mays]





> gi|195608344|gb|ACG26002.1|





hydrogen-transporting





ATP synthase, rotational





mechanism [Zea mays]





> gi|195647528|gb|ACG43232.1|





hydrogen-transporting





ATP synthase, rotational





mechanism [Zea mays]





> gi|195658703|gb|ACG48819.1|





hydrogen-transporting





ATP synthase, rotational





mechanism [Zea mays]




ACF82609
unknown [Zea mays]
0.93
1303
1931




NP_001149611
LOC100283237 [Zea mays]
0.93
1304
1932





> gi|194696248|gb|ACF82208.1|





unknown [Zea mays]





> gi|195605550|gb|ACG24605.1|





hydrogen-transporting





ATP synthase, rotational





mechanism [Zea mays]





> gi|195619622|gb|ACG31641.1|





hydrogen-transporting





ATP synthase, rotational





mechanism [Zea mays]





> gi|195628500|gb|ACG36080.1|





hydrogen-transporting





ATP synthase, rotational





mechanism [Zea mays]





> gi|195637444|gb|ACG38190.1|





hydrogen-transporting





ATP synthase, rotational





mechanism [Zea mays]





> gi|195644406|gb|ACG41671.1|





hydrogen-transporting





ATP synthase, rotational





mechanism [Zea mays]




BAJ87122
predicted protein [Hordeum
0.89
1305
1933





vulgare subsp. vulgare]




NP_001043488
Os01g0600000 [Oryza sativa
0.88
1306
1934





Japonica Group]





> gi|113533019|dbj|BAF05402.1|





Os01g0600000 [Oryza





sativa Japonica Group]





> gi|125571065|gb|EAZ12580.1|





hypothetical protein





OsJ_02485 [Oryza sativa





Japonica Group]





> gi|215768142|dbj|BAH00371.1|





unnamed protein





product [Oryza sativa Japonica





Group]





> gi|218188591|gb|EEC71018.1|





hypothetical protein





OsI_02711 [Oryza sativa





Indica Group]




ABF70110
mitochondrial ATP synthase g
0.78
1307
1935





subunit family protein [Musabalbisiana]




ABA40451
unknown [Solanum
0.72
1308
1936





tuberosum]




NP_001056144
Os05g0533800 [Oryza sativa
0.73
1309
1937





Japonica Group]





> gi|48843828|gb|AAT47087.1|





unknown protein [Oryza





sativa Japonica Group]





> gi|113579695|dbj|BAF18058.1|





Os05g0533800 [Oryza





sativa Japonica Group]





> gi|222632347|gb|EEE64479.1|





hypothetical protein





OsJ_19329 [Oryza sativa





Japonica Group]




BAJ90534
predicted protein [Hordeum
0.74
1310
1938





vulgare subsp. vulgare]


ath-
742-763
ACN26323
unknown [Zea mays]
1.00
1389
2005


miR2936


> gi|223944533|gb|ACN26350.1|





unknown [Zea mays]




BAC01259
glycosylphosphatidylinositol
0.78
1390
2006





anchor attachment 1-like





[Oryza sativa Japonica Group]





> gi|125571593|gb|EAZ13108.1|





hypothetical protein





OsJ_03027 [Oryza sativa





Japonica Group]




EAY75389
hypothetical protein
0.78
1391





OsI_03287 [Oryza sativa





Indica Group]





Table 6: Provided are the target Genes of miRNAs Associated with Increased NUE (Table 2) along with their GenBank Accession numbers and sequence identifiers (SEQ ID NO:).


“bind” = binding;


“pos” = position;


“hom” = homologue;


“p.p.” = polypeptide;


“p.n.” = polynucleotide.













TABLE 7







Target genes of up regulated siRNA sequences associated with NUE


(Table 3)



















p.n.



Mir
Hom.


p.p.
SEQ


Mir
Bind.
NCBI


SEQ ID
ID


name
Pos.
Accession
Annotation/organism
Identity
NO:
NO:
















Predicted
1180-1201
XP_002439783
hypothetical protein
1.00
2007
2437


siRNA


SORBIDRAFT_09g019980





59952


[Sorghum bicolor]








> gi|241945068|gb|EES18213.1|








hypothetical protein








SORBIDRAFT_09g019980








[Sorghum bicolor]







NP_001140772
hypothetical protein
0.87
2008
2438





LOC100272847 [Zea mays]








> gi|194701020|gb|ACF84594.1|








unknown [Zea mays]







ACG40649
hypothetical protein [Zea mays]
0.87
2009
2439




NP_001140587
hypothetical protein
0.85
2010
2440





LOC100272657 [Zea mays]








> gi|194700088|gb|ACF84128.1|








unknown [Zea mays]







ACG28586
hypothetical protein [Zea mays]
0.83
2011
2441




EEC79208
hypothetical protein OsI_19925
0.73
2012






[Oryza sativa Indica Group]






2775-2796
NP_001146628
hypothetical protein
1.00
2013
2442





LOC100280226 [Zea mays]








> gi|219888087|gb|ACL54418.1|








unknown [Zea mays]







EEE65008
hypothetical protein OsJ_19956
0.71
2014






[Oryza sativa Japonica Group]







BAA90807
putative SEU1 protein [Oryza
0.71
2015
2443





sativa Japonica Group]








> gi|215704489|dbj|BAG93923.1|








unnamed protein product








[Oryza sativa Japonica Group]





Predicted
220-238
ACR33998
unknown [Zea mays]
1.00
2016
2444


siRNA








59626










NP_001141472
hypothetical protein
1.00
2017
2445





LOC100273582 [Zea mays]








> gi|194704716|gb|ACF86442.1|








unknown [Zea mays]






1435-1453
XP_002446603
hypothetical protein
1.00
2018
2446





SORBIDRAFT_06g018770








[Sorghum bicolor]








> gi|241937786|gb|EES10931.1|








hypothetical protein








SORBIDRAFT_06g018770








[Sorghum bicolor]







NP_001159223
hypothetical protein
0.92
2019
2447





LOC100304309 [Zea mays]








> gi|223942807|gb|ACN25487.1|








unknown [Zea mays]







BAJ94860
predicted protein [Hordeum
0.79
2020
2448





vulgare subsp. vulgare]







CAE03375
OSJNBa0036B21.17 [Oryza
0.80
2021






sativa Japonica Group]







EEE61116
hypothetical protein OsJ_15040
0.80
2022






[Oryza sativa Japonica Group]







CBW45773
ORW1943Ba0077G13.1 [Oryza
0.80
2023






rufipogon]







NP_001052976
Os04g0458200 [Oryza sativa
0.80
2024
2449





Japonica Group]








> gi|215715295|dbj|BAG95046.1|








unnamed protein product








[Oryza sativa Japonica Group]








> gi|218194967|gb|EEC77394.1|








hypothetical protein OsI_16151








[Oryza sativa Indica Group]








> gi|255675525|dbj|BAF14890.2|








Os04g0458200 [Oryza sativa








Japonica Group]







CAH67071
OSIGBa0097P08.1 [Oryza sativa
0.79
2025
2450





Indica Group]






1613-1631
NP_001141472
hypothetical protein
1.00
2026
2451





LOC100273582 [Zea mays]








> gi|194704716|gb|ACF86442.1|








unknown [Zea mays]







ACR33998
unknown [Zea mays]
0.85
2027
2452


Predicted
408-428
NP_001131645
hypothetical protein
1.00
2028
2453


siRNA


LOC100193004 [Zea mays]





60850


> gi|194692138|gb|ACF80153.1|








unknown [Zea mays]






481-501
ACG31592
hypothetical protein [Zea mays]
1.00
2029
2454




NP_001132267
hypothetical protein
1.00
2030
2455





LOC100193703 [Zea mays]








> gi|194693920|gb|ACF81044.1|








unknown [Zea mays]





Predicted
1611-1630
XP_002463038
hypothetical protein
1.00
2031
2456


siRNA


SORBIDRAFT_02g036610





59961


[Sorghum bicolor]








> gi|241926415|gb|EER99559.1|








hypothetical protein








SORBIDRAFT_02g036610








[Sorghum bicolor]







NP_001130320
hypothetical protein
0.96
2032
2457





LOC100191414 [Zea mays]








> gi|194688838|gb|ACF78503.1|








unknown [Zea mays]







BAJ88611
predicted protein [Hordeum
0.87
2033
2458





vulgare subsp. vulgare]







NP_001060016
Os07g0566200 [Oryza sativa
0.89
2034
2459





Japonica Group]








> gi|75152457|sp|Q8H4S6.2|P2C64_ORYSJ








RecName:








Full = Probable protein








phosphatase 2C 64;








Short = OsPP2C64








> gi|33146759|dbj|BAC79670.1|








putative protein phosphatase 2C








[Oryza sativa Japonica Group]








> gi|113611552|dbj|BAF21930.1|








Os07g0566200 [Oryza sativa








Japonica Group]








> gi|125600752|gb|EAZ40328.1|








hypothetical protein OsJ_24776








[Oryza sativa Japonica Group]







NP_001150759
LOC100284392 [Zea mays]
0.92
2035
2460





> gi|195641568|gb|ACG40252.1|








catalytic/protein phosphatase








type 2C [Zea mays]







ACR38208
unknown [Zea mays]
0.92
2036
2461




EAZ04367
hypothetical protein OsI_26509
0.88
2037






[Oryza sativa Indica Group]







ACN30598
unknown [Zea mays]
0.87
2038
2462




ACF84784
unknown [Zea mays]
0.80
2039
2463



802-821
XP_002462759
hypothetical protein
1.00
2040
2464





SORBIDRAFT_02g031500








[Sorghum bicolor]








> gi|241926136|gb|EER99280.1|








hypothetical protein








SORBIDRAFT_02g031500








[Sorghum bicolor]







NP_001151289
RING-H2 finger protein ATL2B
0.80
2041
2465





[Zea mays]








> gi|195645562|gb|ACG42249.1|








RING-H2 finger protein








ATL2B [Zea mays]








> gi|223946553|gb|ACN27360.1|








unknown [Zea mays]






540-559
XP_002438439
hypothetical protein
1.00
2042
2466





SORBIDRAFT_10g019640








[Sorghum bicolor]








> gi|241916662|gb|EER89806.1|








hypothetical protein








SORBIDRAFT_10g019640








[Sorghum bicolor]







ACN33733
unknown [Zea mays]
0.89
2043
2467




NP_001150669
amino acid permease [Zea mays]
0.89
2044
2468





> gi|195640964|gb|ACG39950.1|








amino acid permease [Zea








mays]







XP_002453115
hypothetical protein
0.82
2045
2469





SORBIDRAFT_04g000290








[Sorghum bicolor]








> gi|241932946|gb|EES06091.1|








hypothetical protein








SORBIDRAFT_04g000290








[Sorghum bicolor]







NP_001136459
hypothetical protein
0.80
2046
2470





LOC100216569 [Zea mays]








> gi|194695786|gb|ACF81977.1|








unknown [Zea mays]







NP_001045585
Os02g0101000 [Oryza sativa
0.78
2047
2471





Japonica Group]








> gi|41053220|dbj|BAD08181.1|








putative amino acid transport








protein [Oryza sativa Japonica








Group]








> gi|113535116|dbj|BAF07499.1|








Os02g0101000 [Oryza sativa








Japonica Group]








> gi|215704334|dbj|BAG93768.1|








unnamed protein product








[Oryza sativa Japonica Group]








> gi|218189857|gb|EEC72284.1|








hypothetical protein OsI_05452








[Oryza sativa Indica Group]








> gi|222621988|gb|EEE56120.1|








hypothetical protein OsJ_04987








[Oryza sativa Japonica Group]







BAJ96557
predicted protein [Hordeum
0.73
2048
2472





vulgare subsp. vulgare]








> gi|326523625|dbj|BAJ92983.1|








predicted protein [Hordeum








vulgare subsp. vulgare]







XP_002446139
hypothetical protein
0.72
2049
2473





SORBIDRAFT_06g002240








[Sorghum bicolor]








> gi|241937322|gb|EES10467.1|








hypothetical protein








SORBIDRAFT_06g002240








[Sorghum bicolor]







NP_001147785
amino acid permease [Zea mays]
0.71
2050
2474





> gi|195613758|gb|ACG28709.1|








amino acid permease [Zea








mays]






987-1006
NP_001132785
hypothetical protein
1.00
2051
2475





LOC100194274 [Zea mays]








> gi|194695392|gb|ACF81780.1|








unknown [Zea mays]







NP_001150926
bHLH transcription factor GBOF-
0.96
2052
2476





1 [Zea mays]








> gi|195642998|gb|ACG40967.1|








bHLH transcription factor








GBOF-1 [Zea mays]







XP_002462650
hypothetical protein
0.79
2053
2477





SORBIDRAFT_02g029530








[Sorghum bicolor]








> gi|241926027|gb|EER99171.1|








hypothetical protein








SORBIDRAFT_02g029530








[Sorghum bicolor]







NP_001149110
LOC100282732 [Zea mays]
0.76
2054
2478





> gi|195624818|gb|ACG34239.1|








bHLH transcription factor








GBOF-1 [Zea mays]







ACR35934
unknown [Zea mays]
0.75
2055
2479



235-254
NP_001065691
Os11g0136600 [Oryza sativa
1.00

2480





Japonica Group]








> gi|33340240|gb|AAQ14593.1|








AF319480_1 calcium-dependent








calmodulin-independent protein








kinase [Oryza sativa]








> gi|33340242|gb|AAQ14594.1|








AF319481_1 calcium-dependent








calmodulin-independent protein








kinase [Oryza sativa]








> gi|77548548|gb|ABA91345.1|








Calcium-dependent protein








kinase, isoform 2, putative,








expressed [Oryza sativa Japonica








Group]








> gi|113644395|dbj|BAF27536.1|








Os11g0136600 [Oryza sativa








Japonica Group]





Predicted
452-472
XP_002465086
hypothetical protein
1.00
2056
2481


siRNA


SORBIDRAFT_01g031850





56542


[Sorghum bicolor]








> gi|241918940|gb|EER92084.1|








hypothetical protein








SORBIDRAFT_01g031850








[Sorghum bicolor]







NP_001145189
hypothetical protein
0.92
2057
2482





LOC100278439 [Zea mays]








> gi|195652469|gb|ACG45702.1|








hypothetical protein [Zea mays]







NP_001050454
Os03g0439700 [Oryza sativa
0.78
2058
2483





Japonica Group]








> gi|108709042|gb|ABF96837.1|








expressed protein [Oryza sativa








Japonica Group]








> gi|113548925|dbj|BAF12368.1|








Os03g0439700 [Oryza sativa








Japonica Group]








> gi|222625197|gb|EEE59329.1|








hypothetical protein OsJ_11404








[Oryza sativa Japonica Group]







AAR89010
expressed protein [Oryza sativa
0.77
2059






Japonica Group]







BAJ96331
predicted protein [Hordeum
0.77
2060
2484





vulgare subsp. vulgare]





Predicted
585-608
NP_001183654
hypothetical protein
1.00
2061
2485


siRNA


LOC100502248 [Zea mays]





56353


> gi|238013682|gb|ACR37876.1|








unknown [Zea mays]







XP_002464467
hypothetical protein
0.94
2062
2486





SORBIDRAFT_01g018940








[Sorghum bicolor]








> gi|241918321|gb|EER91465.1|








hypothetical protein








SORBIDRAFT_01g018940








[Sorghum bicolor]







AAL59033
hypothetical protein [Oryza sativa
0.78
2063






Japonica Group]








> gi|31432747|gb|AAP54340.1|








expressed protein [Oryza sativa








Japonica Group]







BAJ89715
predicted protein [Hordeum
0.77
2064
2487





vulgare subsp. vulgare]








> gi|326505214|dbj|BAK02994.1|








predicted protein [Hordeum








vulgare subsp. vulgare]







BAK03788
predicted protein [Hordeum
0.75
2065
2488





vulgare subsp. vulgare]







NP_001064892
Os10g0484900 [Oryza sativa
0.76
2066
2489





Japonica Group]








> gi|255679504|dbj|BAF26806.2|








Os10g0484900 [Oryza sativa








Japonica Group]






23-46
XP_002463222
hypothetical protein
1.00
2067
2490





SORBIDRAFT_02g040060








[Sorghum bicolor]








> gi|241926599|gb|EER99743.1|








hypothetical protein








SORBIDRAFT_02g040060








[Sorghum bicolor]







NP_001105697
thiazole biosynthetic enzyme 1-2,
0.93
2068
2491





chloroplastic precursor [Zea








mays]








> gi|2501190|sp|Q41739.1|THI42_MAIZE








RecName:








Full = Thiazole biosynthetic








enzyme 1-2, chloroplastic; Flags:








Precursor








> gi|596080|gb|AAA96739.1|








thiamine biosynthetic enzyme








[Zea mays]







ACF87708
unknown [Zea mays]
0.93
2069
2492





> gi|224033955|gb|ACN36053.1|








unknown [Zea mays]







NP_001105696
thiazole biosynthetic enzyme 1-1,
0.92
2070
2493





chloroplastic precursor [Zea








mays]








> gi|2501189|sp|Q41738.1|THI41_MAIZE








RecName:








Full = Thiazole biosynthetic








enzyme 1-1, chloroplastic; Flags:








Precursor








> gi|596078|gb|AAA96738.1|








thiamine biosynthetic enzyme








[Zea mays]








> gi|194704634|gb|ACF86401.1|








unknown [Zea mays]







NP_001059841
Os07g0529600 [Oryza sativa
0.87
2071
2494





Japonica Group]








> gi|32352138|dbj|BAC78562.1|








thiamine biosynthetic enzyme








[Oryza sativa Japonica Group]








> gi|113611377|dbj|BAF21755.1|








Os07g0529600 [Oryza sativa








Japonica Group]








> gi|215712225|dbj|BAG94352.1|








unnamed protein product








[Oryza sativa Japonica Group]







BAC45141
putative thiamine biosynthesis
0.87
2072
2495





protein [Oryza sativa Japonica








Group]








> gi|125600511|gb|EAZ40087.1|








hypothetical protein OsJ_24530








[Oryza sativa Japonica Group]







EAZ04139
hypothetical protein OsI_26282
0.87
2073






[Oryza sativa Indica Group]







XP_002458014
hypothetical protein
0.91
2074
2496





SORBIDRAFT_03g025520








[Sorghum bicolor]








> gi|241929989|gb|EES03134.1|








hypothetical protein








SORBIDRAFT_03g025520








[Sorghum bicolor]







ACF85034
unknown [Zea mays]
0.84
2075
2497




AAZ93636
pathogen-induced defense-
0.84
2076
2498





responsive protein 8 [Oryza sativa








Indica Group]






71-94
ACG31888
ubiquitin-conjugating enzyme E2
1.00
2077
2499





W [Zea mays]







NP_001050906
Os03g0681400 [Oryza sativa
0.71
2078
2500





Japonica Group]








> gi|57164486|gb|AAK00965.2|








AC079736_5 putative ubiquitin-








conjugating enzyme E2 [Oryza








sativa Japonica Group]








> gi|108710422|gb|ABF98217.1|








Ubiquitin-conjugating enzyme








family protein, expressed [Oryza








sativa Japonica Group]








> gi|113549377|dbj|BAF12820.1|








Os03g0681400 [Oryza sativa








Japonica Group]








> gi|215737341|dbj|BAG96270.1|








unnamed protein product








[Oryza sativa Japonica Group]








> gi|222625566|gb|EEE59698.1|








hypothetical protein OsJ_12122








[Oryza sativa Japonica Group]







XP_002464080
hypothetical protein
0.72
2079
2501





SORBIDRAFT_01g011940








[Sorghum bicolor]








> gi|241917934|gb|EER91078.1|








hypothetical protein








SORBIDRAFT_01g011940








[Sorghum bicolor]







XP_002443656
hypothetical protein
0.71
2080
2502





SORBIDRAFT_08g022990








[Sorghum bicolor]








> gi|241944349|gb|EES17494.1|








hypothetical protein








SORBIDRAFT_08g022990








[Sorghum bicolor]







NP_001146940
ubiquitin-conjugating enzyme E2
0.70
2081
2503





W [Zea mays]








> gi|195605468|gb|ACG24564.1|








ubiquitin-conjugating enzyme








E2 W [Zea mays]





Predicted
614-634
NP_001047323
Os02g0596900 [Oryza sativa
1.00
2082
2504


siRNA


Japonica Group]





59965


> gi|75291091|sp|Q6K908.1|ARP3_ORYSJ








RecName:








Full = Actin-related protein 3








> gi|190356069|sp|A2X6S3.2|ARP3_ORYSI








RecName:








Full = Actin-related protein 3








> gi|47847830|dbj|BAD21625.1|








putative arp3 [Oryza sativa








Japonica Group]








> gi|113536854|dbj|BAF09237.1|








Os02g0596900 [Oryza sativa








Japonica Group]








> gi|218191098|gb|EEC73525.1|








hypothetical protein OsI_07915








[Oryza sativa Indica Group]








> gi|222623169|gb|EEE57301.1|








hypothetical protein OsJ_07378








[Oryza sativa Japonica Group]







ACF87749
unknown [Zea mays]
0.92
2083
2505





> gi|223972725|gb|ACN30550.1|








unknown [Zea mays]







XP_002285370
PREDICTED: hypothetical
0.86
2084
2506





protein [Vitis vinifera]







NP_001147580
LOC100281189 [Zea mays]
0.92
2085
2507





> gi|195612296|gb|ACG27978.1|








actin-like protein 3 [Zea mays]







NP_001170315
hypothetical protein
0.90
2086
2508





LOC100384280 [Zea mays]








> gi|224034999|gb|ACN36575.1|








unknown [Zea mays]







XP_002315648
predicted protein [Populus
0.84
2087
2509





trichocarpa]








> gi|222864688|gb|EEF01819.1|








predicted protein [Populus








trichocarpa]







XP_002515041
protein binding protein, putative
0.85
2088
2510





[Ricinus communis]








> gi|223546092|gb|EEF47595.1|








protein binding protein, putative








[Ricinus communis]







XP_002312654
predicted protein [Populus
0.84
2089
2511





trichocarpa]








> gi|222852474|gb|EEE90021.1|








predicted protein [Populus








trichocarpa]







CAM97883
actin-related protein 3 [Nicotiana
0.83
2090
2512





tabacum]







NP_172777
actin-related protein 3
0.81
2091
2513





[Arabidopsis thaliana]








> gi|75313139|sp|Q9SAF1.1|ARP3_ARATH








RecName:








Full = Actin-related protein 3;








AltName: Full = Protein








DISTORTED TRICHOMES 1








> gi|4850401|gb|AAD31071.1|








AC007357_20 Strong similarity








to gb|U29610 Actin-like protein








(Arp3) from Acanthamoeba








castellanii and is a member of the








PF|00022 Actin family








[Arabidopsis thaliana]








> gi|21427461|gb|AAM53243.1|








AF507911_1 actin-related protein








3 [Arabidopsis thaliana]








> gi|20260500|gb|AAM13148.1|








similar to actin-like protein








[Arabidopsis thaliana]








> gi|21489929|tpg|DAA00033.1|








TPA_exp: actin-related protein








3; AtARP3 [Arabidopsis thaliana]








> gi|30387525|gb|AAP31928.1|








At1g13180 [Arabidopsis thaliana]








> gi|332190859|gb|AEE28980.1|








actin-related protein 3








[Arabidopsis thaliana]





Predicted
632-651
ACR34392
unknown [Zea mays]
1.00
2092
2514


siRNA








58872









315-334
NP_001004443
beta-hexosaminidase subunit
1.00
2093
2515





alpha precursor [Rattus








norvegicus]








> gi|85701350|sp|Q641X3.1|HEXA_RAT








RecName: Full = Beta-








hexosaminidase subunit alpha;








AltName: Full = Beta-N-








acetylhexosaminidase subunit








alpha; Short = Hexosaminidase








subunit A; AltName: Full = N-








acetyl-beta-glucosaminidase








subunit alpha; Flags: Precursor








> gi|51980341|gb|AAH82097.1|








Hexosaminidase A [Rattus








norvegicus]








> gi|149041858|gb|EDL95699.1|








hexosaminidase A, isoform








CRA_a [Rattus norvegicus]







NP_034551
beta-hexosaminidase subunit
0.94
2094
2516





alpha precursor [Mus musculus]








> gi|497174|gb|AAA18775.1|








beta-hexosaminidase [Mus








musculus]








> gi|497196|gb|AAA18777.1|








beta-hexosaminidase alpha-








subunit [Mus musculus]








> gi|14789650|gb|AAH10755.1|








Hexosaminidase A [Mus








musculus]








> gi|26344774|dbj|BAC36036.1|








unnamed protein product [Mus








musculus]








> gi|26344800|dbj|BAC36049.1|








unnamed protein product [Mus








musculus]








> gi|74184438|dbj|BAE25744.1|








unnamed protein product [Mus








musculus]








> gi|74204649|dbj|BAE35394.1|








unnamed protein product [Mus








musculus]








> gi|148694024|gb|EDL25971.1|








hexosaminidase A [Mus








musculus]







BAE35636
unnamed protein product [Mus
0.94
2095
2517





musculus]







BAE29566
unnamed protein product [Mus
0.94
2096
2518





musculus]







BAE30831
unnamed protein product [Mus
0.94
2097
2519





musculus]







BAE35457
unnamed protein product [Mus
0.94
2098
2520





musculus]







P29416
RecName: Full = Beta-
0.94
2099






hexosaminidase subunit alpha;








AltName: Full = Beta-N-








acetylhexosaminidase subunit








alpha; Short = Hexosaminidase








subunit A; AltName: Full = N-








acetyl-beta-glucosaminidase








subunit alpha; Flags: Precursor








> gi|51265|emb|CAA45615.1|








beta-N-acetylhexosaminidase








[Mus musculus]








> gi|577688|gb|AAC53246.1|








beta-N-acetylhexosaminidase,








alpha-subunit [Mus musculus]







BAC38018
unnamed protein product [Mus
0.94
2100
2521





musculus]







BAE39189
unnamed protein product [Mus
0.94
2101
2522





musculus]







BAE40146
unnamed protein product [Mus
0.94
2102
2523





musculus]






535-554
XP-002526446
heat shock protein, putative
1.00
2103
2524





[Ricinus communis]








> gi|223534226|gb|EEF35941.1|








heat shock protein, putative








[Ricinus communis]







XP_002328713
predicted protein [Populus
0.92
2104
2525





trichocarpa]








> gi|222839011|gb|EEE77362.1|








predicted protein [Populus








trichocarpa]







XP_002331133
predicted protein [Populus
0.92
2105
2526





trichocarpa]








> gi|222872861|gb|EEF09992.1|








predicted protein [Populus








trichocarpa]







XP_002279101
PREDICTED: hypothetical
0.93
2106
2527





protein [Vitis vinifera]







ABZ04081
chloroplast heat shock protein 70-
0.90
2107
2528





2 [Ipomoea nil]







ABM92419
chloroplast HSP70 [Cucumis
0.90
2108
2529





sativus]







ABE79560
Chaperone DnaK [Medicago
0.90
2109
2530





truncatula]







CAA52149
heat shock protein 70 [Cucumis
0.89
2110
2531





sativus]







Q02028
RecName: Full = Stromal 70 kDa
0.90
2111






heat shock-related protein,








chloroplastic; Flags: Precursor








> gi|169023|gb|AAA33637.1|








70 kDa heat shock protein [Pisum








sativum]








> gi|871515|emb|CAA49147.1|








Psst70 (stress 70 protein) [Pisum








sativum]







CAN81065
hypothetical protein
0.91
2112
2532





VITISV_000728 [Vitis vinifera]





Predicted
1842-1866
YP_024387
ribulose-1,5-bisphosphate
1.00
2113
2533


siRNA


carboxylase/oxygenase large





54631


subunit [Saccharum hybrid








cultivar SP-80-3280]








> gi|50812536|ref|YP_054639.1|








ribulose-1,5-bisphosphate








carboxylase/oxygenase large








subunit [Saccharum officinarum]








> gi|118614500|ref|YP_899415.1|








ribulose 1,5-bisphosphate








carboxylase/oxygenase large








subunit [Sorghum bicolor]








> gi|75290174|sp|Q6ENV5.1|RBL_SACOF








RecName:








Full = Ribulose bisphosphate








carboxylase large chain;








Short = RuBisCO large subunit;








Flags: Precursor








> gi|75291223|sp|Q6L391.1|RBL_SACHY








RecName:








Full = Ribulose bisphosphate








carboxylase large chain;








Short = RuBisCO large subunit;








Flags: Precursor








> gi|125987520|sp|A1E9T2.1|RBL_SORBI








RecName:








Full = Ribulose bisphosphate








carboxylase large chain;








Short = RuBisCO large subunit;








Flags: Precursor








> gi|48478681|gb|AAT44701.1|








RuBisCO large chain [Saccharum








hybrid cultivar SP80-3280]








> gi|49659520|dbj|BAD27301.1|








RuBisCO large subunit








[Saccharum hybrid cultivar NCo








310]








> gi|118201134|gb|ABK79504.1|








ribulose-1,5-bisphosphate








carboxylase/oxygenase large








subunit [Sorghum bicolor]







NP_043033
ribulose-1,5-bisphosphate
0.99
2114
2534





carboxylase/oxygenase large








subunit [Zea mays]








> gi|1172861|sp|P00874.2|RBL_MAIZE








RecName:








Full = Ribulose bisphosphate








carboxylase large chain;








Short = RuBisCO large subunit;








Flags: Precursor








> gi|18036|emb|CAA78027.1|








Ribulose bisphosphate








carboxylase [Zea mays]








> gi|902230|emb|CAA60294.1|








ribulose-1,5-bisphosphate








carboxylase/oxygenase large








subunit [Zea mays]







ABP01447
ribulose-1,5-bisphosphate
0.99
2115
2535





carboxylase/oxygenase large








subunit [Sorghastrum nutans]







YP_003208195
ribulose-1,5-bisphosphate
0.98
2116
2536





carboxylase/oxygenase large








subunit [Coix lacryma-jobi]








> gi|209361365|gb|ACI43280.1|








ribulose-1,5-bisphosphate








carboxylase/oxygenase large








subunit [Coix lacryma-jobi]







ABP01443
ribulose-1,5-bisphosphate
0.98
2117
2537





carboxylase/oxygenase large








subunit [Cymbopogon citratus]







ABP01445
ribulose-1,5-bisphosphate
0.98
2118
2538





carboxylase/oxygenase large








subunit [Pogonatherum sp.








Hodkinson 21]







ABP01442
ribulose-1,5-bisphosphate
0.97
2119
2539





carboxylase/oxygenase large








subunit [Coix lacryma-jobi]







ABP01448
ribulose-1,5-bisphosphate
0.96
2120
2540





carboxylase/oxygenase large








subunit [Sorghum halepense]







CAG34130
ribulose-1,5-bisphosphate
0.96
2121
2541





carboxylase/oxygenase large








subunit [Arthraxon sp. Hodkinson








111]







AAA31678
ribulose 1,5-bisphosphate
0.97
2122
2542





carboxylase/oxygenase [Cenchrus








setiger]








> gi|294104|gb|AAA32020.1|








ribulosebiphosphate carboxylase,








large subunit [Pennisetum








glaucum]






884-908
ABP01449
ribulose-1,5-bisphosphate
1.00
2123
2543





carboxylase/oxygenase large








subunit [Trachypogon spicatus]







ABP01447
ribulose-1,5-bisphosphate
1.00
2124
2544





carboxylase/oxygenase large








subunit [Sorghastrum nutans]







NP_043033
ribulose-1,5-bisphosphate
0.99
2125
2545





carboxylase/oxygenase large








subunit [Zea mays]








> gi|1172861|sp|P00874.2|RBL_MAIZE








RecName:








Full = Ribulose bisphosphate








carboxylase large chain;








Short = RuBisCO large subunit;








Flags: Precursor








> gi|18036|emb|CAA78027.1|








Ribulose bisphosphate








carboxylase [Zea mays]








> gi|902230|emb|CAA60294.1|








ribulose-1,5-bisphosphate








carboxylase/oxygenase large








subunit [Zea mays]







YP_024387
ribulose-1,5-bisphosphate
1.00
2126
2546





carboxylase/oxygenase large








subunit [Saccharum hybrid








cultivar SP-80-3280]








> gi|50812536|ref|YP_054639.1|








ribulose-1,5-bisphosphate








carboxylase/oxygenase large








subunit [Saccharum officinarum]








> gi|118614500|ref|YP_899415.1|








ribulose 1,5-bisphosphate








carboxylase/oxygenase large








subunit [Sorghum bicolor]








> gi|75290174|sp|Q6ENV5.1|RBL_SACOF








RecName:








Full = Ribulose bisphosphate








carboxylase large chain;








Short = RuBisCO large subunit;








Flags: Precursor








> gi|75291223|sp|Q6L391.1|RBL_SACHY








RecName:








Full = Ribulose bisphosphate








carboxylase large chain;








Short = RuBisCO large subunit;








Flags: Precursor








> gi|125987520|sp|A1E9T2.1|RBL_SORBI








RecName:








Full = Ribulose bisphosphate








carboxylase large chain;








Short = RuBisCO large subunit;








Flags: Precursor








> gi|48478681|gb|AAT44701.1|








RuBisCO large chain [Saccharum








hybrid cultivar SP80-3280]








> gi|49659520|dbj|BAD27301.1|








RuBisCO large subunit








[Saccharum hybrid cultivar NCo








310]








> gi|118201134|gb|ABK79504.1|








ribulose-1,5-bisphosphate








carboxylase/oxygenase large








subunit [Sorghum bicolor]







ABP01443
ribulose-1,5-bisphosphate
1.00
2127
2547





carboxylase/oxygenase large








subunit [Cymbopogon citratus]







ABP01445
ribulose-1,5-bisphosphate
0.99
2128
2548





carboxylase/oxygenase large








subunit [Pogonatherum sp.








Hodkinson 21]







ABP01442
ribulose-1,5-bisphosphate
0.98
2129
2549





carboxylase/oxygenase large








subunit [Coix lacryma-jobi]







YP_003208195
ribulose-1,5-bisphosphate
0.98
2130
2550





carboxylase/oxygenase large








subunit [Coix lacryma-jobi]








> gi|209361365|gb|ACI43280.1|








ribulose-1,5-bisphosphate








carboxylase/oxygenase large








subunit [Coix lacryma-jobi]







ABP01441
ribulose-1,5-bisphosphate
0.98
2131
2551





carboxylase/oxygenase large








subunit [Bothriochloa








ischaemum]







CAG34130
ribulose-1,5-bisphosphate
0.96
2132
2552





carboxylase/oxygenase large








subunit [Arthraxon sp. Hodkinson








111]





Predicted
69-92
XP_002458082
hypothetical protein
1.00
2133
2553


siRNA


SORBIDRAFT_03g026670





59846


[Sorghum bicolor]








> gi|241930057|gb|EES03202.1|








hypothetical protein








SORBIDRAFT_03g026670








[Sorghum bicolor]







EEC70994
hypothetical protein OsI_02661
0.83
2134






[Oryza sativa Indica Group]







NP_001152397
regulator of telomere elongation
0.78
2135
2554





helicase 1 [Zea mays]








> gi|195655863|gb|ACG47399.1|








regulator of telomere








elongation helicase 1 [Zea mays]







NP_001043456
Os01g0592900 [Oryza sativa
0.70
2136
2555





Japonica Group]








> gi|53791584|dbj|BAD52706.1|








DEAH helicase isoform 5-like








[Oryza sativa Japonica Group]








> gi|255673416|dbj|BAF05370.2|








Os01g0592900 [Oryza sativa








Japonica Group]






 85-108
ACR34511
unknown [Zea mays]
1.00
2137
2556




NP_001105187
histone acetyltransferase1 [Zea
0.99
2138
2557





mays]








> gi|20977602|gb|AAM28228.1|








histone acetyl transferase [Zea








mays]








> gi|223975399|gb|ACN31887.1|








unknown [Zea mays]







Q8LPU4
RecName: Full = Histone
0.99
2139






acetyltransferase type B catalytic








subunit; AltName: Full = Histone








acetyltransferase HAT B;








AltName: Full = Histone








acetyltransferase HAT-B-p50








> gi|6288802|gb|AAF06742.1|








AF171927_1 histone








acetyltransferase HAT-B-p50








[Zea mays]








> gi|5579441|gb|AAC03423.2|








histone acetyltransferase HAT B








[Zea mays]







XP_002460092
hypothetical protein
0.89
2140
2558





SORBIDRAFT_02g022640








[Sorghum bicolor]








> gi|241923469|gb|EER96613.1|








hypothetical protein








SORBIDRAFT_02g022640








[Sorghum bicolor]







NP_001062946
Os09g0347800 [Oryza sativa
0.77
2141
2559





Japonica Group]








> gi|75121858|sp|Q6ES10.1|HAT1_ORYSJ








RecName:








Full = Probable acetyltransferase








type B catalytic subunit;








AltName: Full = HAT B








> gi|50252375|dbj|BAD28482.1|








putative histone








acetyltransferase HAT B [Oryza








sativa Japonica Group]








> gi|50252405|dbj|BAD28560.1|








putative histone








acetyltransferase HAT B [Oryza








sativa Japonica Group]








> gi|113631179|dbj|BAF24860.1|








Os09g0347800 [Oryza sativa








Japonica Group]








> gi|215678892|dbj|BAG95329.1|








unnamed protein product








[Oryza sativa Japonica Group]







EEC84410
hypothetical protein OsI_30998
0.77
2142






[Oryza sativa Indica Group]







EEE69531
hypothetical protein OsJ_29003
0.77
2143






[Oryza sativa Japonica Group]







BAK05443
predicted protein [Hordeum
0.75
2144
2560





vulgare subsp. vulgare]






 85-108
XP_002460092
hypothetical protein
1.00
2145
2561





SORBIDRAFT_02g022640








[Sorghum bicolor]








> gi|241923469|gb|EER96613.1|








hypothetical protein








SORBIDRAFT_02g022640








[Sorghum bicolor]







NP_001105187
histone acetyltransferase1 [Zea
0.90
2146
2562





mays]








> gi|20977602|gb|AAM28228.1|








histone acetyl transferase [Zea








mays]








> gi|223975399|gb|ACN31887.1|








unknown [Zea mays]







Q8LPU4
RecName: Full = Histone
0.90
2147






acetyltransferase type B catalytic








subunit; AltName: Full = Histone








acetyltransferase HAT B;








AltName: Full = Histone








acetyltransferase HAT-B-p50








> gi|6288802|gb|AAF06742.1|








AF171927_1 histone








acetyltransferase HAT-B-p50








[Zea mays]








> gi|5579441|gb|AAC03423.2|








histone acetyltransferase HAT B








[Zea mays]







NP_001062946
Os09g0347800 [Oryza sativa
0.81
2148
2563





Japonica Group]








> gi|75121858|sp|Q6ES10.1|HAT1_ORYSJ








RecName:








Full = Probable acetyltransferase








type B catalytic subunit;








AltName: Full = HAT B








> gi|50252375|dbj|BAD28482.1|








putative histone








acetyltransferase HAT B [Oryza








sativa Japonica Group]








> gi|50252405|dbj|BAD28560.1|








putative histone








acetyltransferase HAT B [Oryza








sativa Japonica Group]








> gi|113631179|dbj|BAF24860.1|








Os09g0347800 [Oryza sativa








Japonica Group]








> gi|215678892|dbj|BAG95329.1|








unnamed protein product








[Oryza sativa Japonica Group]







EEC84410
hypothetical protein OsI_30998
0.81
2149






[Oryza sativa Indica Group]







EEE69531
hypothetical protein OsJ_29003
0.81
2150






[Oryza sativa Japonica Group]







BAK05443
predicted protein [Hordeum
0.78
2151
2564





vulgare subsp. vulgare]







ACR34511
unknown [Zea mays]
0.79
2152
2565


Predicted
293-311
XP_002460816
hypothetical protein
1.00
2153
2566


siRNA


SORBIDRAFT_02g035420





60081


[Sorghum bicolor]








> gi|241924193|gb|EER97337.1|








hypothetical protein








SORBIDRAFT_02g035420








[Sorghum bicolor]







NP_001147287
receptor-like serine-threonine
0.87
2154
2567





protein kinase [Zea mays]








> gi|195609532|gb|ACG26596.1|








receptor-like serine-threonine








protein kinase [Zea mays]







BAK06110
predicted protein [Hordeum
0.73
2155
2568





vulgare subsp. vulgare]






564-582
ACN27203
unknown [Zea mays]
1.00
2156
2569




NP_001150572
RING-H2 finger protein ATL1R
1.00
2157
2570





[Zea mays]








> gi|195640284|gb|ACG39610.1|








RING-H2 finger protein








ATL1R [Zea mays]







XP_002448400
hypothetical protein
0.80
2158
2571





SORBIDRAFT_06g026580








[Sorghum bicolor]








> gi|241939583|gb|EES12728.1|








hypothetical protein








SORBIDRAFT_06g026580








[Sorghum bicolor]






374-392
NP_001151643
DNA binding protein [Zea mays]
1.00
2159
2572





> gi|195648318|gb|ACG43627.1|








DNA binding protein [Zea








mays]







XP_002468594
hypothetical protein
0.83
2160
2573





SORBIDRAFT_01g048710








[Sorghum bicolor]








> gi|241922448|gb|EER95592.1|








hypothetical protein








SORBIDRAFT_01g048710








[Sorghum bicolor]







NP_001048832
Os03g0127500 [Oryza sativa
0.73
2161
2574





Japonica Group]








> gi|20330751|gb|AAM19114.1|








AC104427_12 Putative bZIP








transcription factor [Oryza sativa








Japonica Group]








> gi|108705971|gb|ABF93766.1|








bZIP family transcription








factor, putative, expressed [Oryza








sativa Japonica Group]








> gi|113547303|dbj|BAF10746.1|








Os03g0127500 [Oryza sativa








Japonica Group]







EAZ25438
hypothetical protein OsJ_09254
0.73
2162






[Oryza sativa Japonica Group]







EAY88362
hypothetical protein OsI_09817
0.73
2163






[Oryza sativa Indica Group]






365-383
XP_002446810
hypothetical protein
1.00
2164
2575





SORBIDRAFT_06g023020








[Sorghum bicolor]








> gi|241937993|gb|EES11138.1|








hypothetical protein








SORBIDRAFT_06g023020








[Sorghum bicolor]







NP_001185810
yellow stripe-like transporter 11
0.90
2165
2576





[Zea mays]








> gi|308210134|gb|ADO20998.1|








yellow stripe-like transporter 11








[Zea mays]







CAH67887
OSIGBa0153E02-
0.79
2166






OSIGBa0093I20.16 [Oryza sativa








Indica Group]








> gi|125549079|gb|EAY94901.1|








hypothetical protein OsI_16701








[Oryza sativa Indica Group]







Q7X660
RecName: Full = Probable metal-
0.79
2167






nicotianamine transporter YSL11;








AltName: Full = Protein YELLOW








STRIPE LIKE 11;








Short = OsYSL11








> gi|32487645|emb|CAE05635.1|








OSJNBa0038O10.1 [Oryza








sativa Japonica Group]








> gi|57834127|emb|CAI44638.1|








OSJNBb0065J09.18 [Oryza








sativa Japonica Group]








> gi|125591037|gb|EAZ31387.1|








hypothetical protein OsJ_15515








[Oryza sativa Japonica Group]







BAE91891
hypothetical protein [Oryza sativa
0.79
2168
2577





Japonica Group]







Q5JQD7
RecName: Full = Probable metal-
0.74
2169






nicotianamine transporter YSL12;








AltName: Full = Protein YELLOW








STRIPE LIKE 12;








Short = OsYSL12








> gi|116310949|emb|CAH67886.1|








OSIGBa0153E02-








OSIGBa0093I20.15 [Oryza sativa








Indica Group]







NP_001185468
yellow stripe-like transporter 12
0.73
2170
2578





[Zea mays]








> gi|308210136|gb|ADO20999.1|








yellow stripe-like transporter 12








[Zea mays]







XP_002452492
hypothetical protein
0.71
2171
2579





SORBIDRAFT_04g026840








[Sorghum bicolor]








> gi|241932323|gb|EES05468.1|








hypothetical protein








SORBIDRAFT_04g026840








[Sorghum bicolor]







BAE91892
hypothetical protein [Oryza sativa
0.73
2172
2580





Japonica Group]







XP_002446809
hypothetical protein
0.72
2173
2581





SORBIDRAFT_06g023010








[Sorghum bicolor]








> gi|241937992|gb|EES11137.1|








hypothetical protein








SORBIDRAFT_06g023010








[Sorghum bicolor]






529-547
XP_002464238
hypothetical protein
1.00
2174
2582





SORBIDRAFT_01g014760








[Sorghum bicolor]








> gi|241918092|gb|EER91236.1|








hypothetical protein








SORBIDRAFT_01g014760








[Sorghum bicolor]







NP_001146578
hypothetical protein
0.92
2175
2583





LOC100280174 [Zea mays]








> gi|219887889|gb|ACL54319.1|








unknown [Zea mays]







ACN27618
unknown [Zea mays]
0.91
2176
2584




EAY90980
hypothetical protein OsI_12589
0.83
2177






[Oryza sativa Indica Group]







NP_001050649
Os03g0609800 [Oryza sativa
0.83
2178
2585





Japonica Group]








> gi|37700299|gb|AAR00589.1|








putative NPH3 family protein








[Oryza sativa Japonica Group]








> gi|40539093|gb|AAR87349.1|








transposon protein, putative,








mutator sub-class [Oryza sativa








Japonica Group]








> gi|62733727|gb|AAX95837.1|








transposon protein, putative,








mutator sub-class [Oryza sativa








Japonica Group]








> gi|108709776|gb|ABF97571.1|








transposon protein, putative,








Mutator sub-class, expressed








[Oryza sativa Japonica Group]








> gi|108709777|gb|ABF97572.1|








transposon protein, putative,








Mutator sub-class, expressed








[Oryza sativa Japonica Group]








> gi|113549120|dbj|BAF12563.1|








Os03g0609800 [Oryza sativa








Japonica Group]








> gi|125587097|gb|EAZ27761.1|








hypothetical protein OsJ_11706








[Oryza sativa Japonica Group]








> gi|215686957|dbj|BAG89762.1|








unnamed protein product








[Oryza sativa Japonica Group]







EAZ21022
hypothetical protein OsJ_36671
0.73
2179






[Oryza sativa Japonica Group]







NP_001067147
Os12g0583500 [Oryza sativa
0.73
2180
2586





Japonica Group]








> gi|77556906|gb|ABA99702.1|








transposon protein, putative,








Mutator sub-class, expressed








[Oryza sativa Japonica Group]








> gi|113649654|dbj|BAF30166.1|








Os12g0583500 [Oryza sativa








Japonica Group]







EAY83679
hypothetical protein OsI_38903
0.73
2181






[Oryza sativa Indica Group]







BAJ87350
predicted protein [Hordeum
0.71
2182
2587





vulgare subsp. vulgare]






3078-3096
NP_001185468
yellow stripe-like transporter 12
1.00
2183
2588





[Zea mays]








> gi|308210136|gb|ADO20999.1|








yellow stripe-like transporter 12








[Zea mays]







XP_002446809
hypothetical protein
0.93
2184
2589





SORBIDRAFT_06g023010








[Sorghum bicolor]








> gi|241937992|gb|EES11137.1|








hypothetical protein








SORBIDRAFT_06g023010








[Sorghum bicolor]







Q5JQD7
RecName: Full = Probable metal-
0.88
2185






nicotianamine transporter YSL12;








AltName: Full = Protein YELLOW








STRIPE LIKE 12;








Short = OsYSL12








> gi|116310949|emb|CAH67886.1|








OSIGBa0153E02-








OSIGBa0093I20.15 [Oryza sativa








Indica Group]







BAE91892
hypothetical protein [Oryza sativa
0.87
2186
2590





Japonica Group]







XP_002452492
hypothetical protein
0.82
2187
2591





SORBIDRAFT_04g026840








[Sorghum bicolor]








> gi|241932323|gb|EES05468.1|








hypothetical protein








SORBIDRAFT_04g026840








[Sorghum bicolor]







BAE44205
hypothetical protein [Oryza sativa
0.81
2188
2592





Japonica Group]







Q6H7J6
RecName: Full = Probable metal-
0.81
2189






nicotianamine transporter YSL14;








AltName: Full = Protein YELLOW








STRIPE LIKE 14;








Short = OsYSL14








> gi|49388177|dbj|BAD25303.1|








oligopeptide transporter OPT-








like [Oryza sativa Japonica








Group]







BAJ89062
predicted protein [Hordeum
0.80
2190
2593





vulgare subsp. vulgare]







BAJ92335
predicted protein [Hordeum
0.80
2191
2594





vulgare subsp. vulgare]







ACN34648
unknown [Zea mays]
0.81
2192
2595





> gi|295441975|gb|ADG21035.1|








oligopeptide transporter [Zea








mays]








> gi|308210138|gb|ADO21000.1|








yellow stripe-like transporter








14A [Zea mays]





Predicted
1927-1945
XP_002450269
hypothetical protein
1.00
2193
2596


siRNA


SORBIDRAFT_05g002900





59380


[Sorghum bicolor]








> gi|241936112|gb|EES09257.1|








hypothetical protein








SORBIDRAFT_05g002900








[Sorghum bicolor]







NP_001130515
hypothetical protein
0.98
2194
2597





LOC100191614 [Zea mays]








> gi|194689354|gb|ACF78761.1|








unknown [Zea mays]








> gi|195635013|gb|ACG36975.1|








pyruvate kinase, cytosolic








isozyme [Zea mays]








> gi|223947285|gb|ACN27726.1|








unknown [Zea mays]








> gi|238009496|gb|ACR35783.1|








unknown [Zea mays]







NP_001149198
LOC100282820 [Zea mays]
0.97
2195
2598





> gi|195625378|gb|ACG34519.1|








pyruvate kinase, cytosolic








isozyme [Zea mays]







BAJ87403
predicted protein [Hordeum
0.95
2196
2599





vulgare subsp. vulgare]







BAJ94667
predicted protein [Hordeum
0.95
2197
2600





vulgare subsp. vulgare]







NP_001065749
Os11g0148500 [Oryza sativa
0.95
2198
2601





Japonica Group]








> gi|77548686|gb|ABA91483.1|








pyruvate kinase family protein,








expressed [Oryza sativa Japonica








Group]








> gi|113644453|dbj|BAF27594.1|








Os11g0148500 [Oryza sativa








Japonica Group]








> gi|215692631|dbj|BAG88051.1|








unnamed protein product








[Oryza sativa Japonica Group]








> gi|218185248|gb|EEC67675.1|








hypothetical protein OsI_35105








[Oryza sativa Indica Group]








> gi|222615524|gb|EEE51656.1|








hypothetical protein OsJ_32969








[Oryza sativa Japonica Group]







NP_001066148
Os12g0145700 [Oryza sativa
0.94
2199
2602





Japonica Group]








> gi|77553678|gb|ABA96474.1|








pyruvate kinase family protein,








expressed [Oryza sativa Japonica








Group]








> gi|113648655|dbj|BAF29167.1|








Os12g0145700 [Oryza sativa








Japonica Group]








> gi|215701048|dbj|BAG92472.1|








unnamed protein product








[Oryza sativa Japonica Group]








> gi|218186432|gb|EEC68859.1|








hypothetical protein OsI_37456








[Oryza sativa Indica Group]








> gi|222616632|gb|EEE52764.1|








hypothetical protein OsJ_35209








[Oryza sativa Japonica Group]







XP_002283911
PREDICTED: hypothetical
0.89
2200
2603





protein [Vitis vinifera]







AAM22747
pyruvate kinase-like
0.88
2201
2604





[Deschampsia antarctica]







ABE80121
Pyruvate kinase [Medicago
0.89
2202






truncatula]





Predicted
43-66
NP_001052992
Os04g0460600 [Oryza sativa
1.00
2203
2605


siRNA


Japonica Group]





55806


> gi|38344965|emb|CAD40985.2|








OSJNBa0072F16.10 [Oryza








sativa Japonica Group]








> gi|108947442|gb|ABF47345.2|








NAC domain protein [Oryza








sativa Japonica Group]








> gi|113564563|dbj|BAF14906.1|








Os04g0460600 [Oryza sativa








Japonica Group]








> gi|116310448|emb|CAH67453.1|








H0219H12.10 [Oryza sativa








Indica Group]








> gi|215692665|dbj|BAG88085.1|








unnamed protein product








[Oryza sativa Japonica Group]








> gi|215737715|dbj|BAG96845.1|








unnamed protein product








[Oryza sativa Japonica Group]








> gi|218194979|gb|EEC77406.1|








hypothetical protein OsI_16171








[Oryza sativa Indica Group]








> gi|222628996|gb|EEE61128.1|








hypothetical protein OsJ_15058








[Oryza sativa Japonica Group]







BAC53811
OsNAC2 protein [Oryza sativa]
0.97
2204
2606




XP_002446618
hypothetical protein
0.71
2205
2607





SORBIDRAFT_06g019010








[Sorghum bicolor]








> gi|241937801|gb|EES10946.1|








hypothetical protein








SORBIDRAFT_06g019010








[Sorghum bicolor]





Predicted
2555-2578
NP_001141674
hypothetical protein
1.00
2206
2608


siRNA


LOC100273800 [Zea mays]





57034


> gi|194705506|gb|ACF86837.1|








unknown [Zea mays]







XP_002451554
hypothetical protein
0.74
2207
2609





SORBIDRAFT_04g003650








[Sorghum bicolor]








> gi|241931385|gb|EES04530.1|








hypothetical protein








SORBIDRAFT_04g003650








[Sorghum bicolor]






95-115
YP_874773
ribosomal protein L14 [Agrostis
1.00
2208
2610





stolonifera]








> gi|118430423|ref|YP_874689.1|








ribosomal protein L14








[Hordeum vulgare subsp. vulgare]








> gi|159106900|ref|YP_001531318.1|








ribosomal protein L14








[Lolium perenne]








> gi|125987532|sp|A1EA45.1|RK14_AGRST








RecName:








Full = 50S ribosomal protein L14,








chloroplastic








> gi|218546804|sp|A1E9M7.1|








RK14_HORVU RecName:








Full = 50S ribosomal protein L14,








chloroplastic








> gi|218546811|sp|A8Y9C3.1|RK14_LOLPR








RecName:








Full = 50S ribosomal protein L14,








chloroplastic








> gi|118201077|gb|ABK79448.1|








ribosomal protein L14








[Hordeum vulgare subsp. vulgare]








> gi|118201247|gb|ABK79616.1|








ribosomal protein L14








[Agrostis stolonifera]








> gi|158934434|emb|CAO86012.1|








ribosomal protein L14








[Lolium perenne]







YP_002364534
ribosomal protein L14 [Festuca
0.99
2209
2611





arundinacea]








> gi|215882361|gb|ACJ70791.1|








ribosomal protein L14 [Festuca








arundinacea]







NP_114294
ribosomal protein L14 [Triticum
0.99
2210
2612





aestivum]








> gi|45477200|sp|Q95H51.1|RK14_WHEAT








RecName:








Full = 50S ribosomal protein L14,








chloroplastic








> gi|13928241|dbj|BAB47070.1|








ribosomal protein L14 [Triticum








aestivum]







NP_039422
ribosomal protein L14 [Oryza
0.98
2211
2613





sativa Japonica Group]








> gi|50234008|ref|YP_052786.1|








ribosomal protein L14 [Oryza








nivara]








> gi|109156621|ref|YP_654240.1|








ribosomal protein L14 [Oryza








sativa Indica Group]








> gi|253729591|ref|YP_003029775.1|








ribosomal protein L14








[Bambusa oldhamii]








> gi|255961418|ref|YP_003097611.1|








ribosomal protein L14








[Dendrocalamus latiflorus]








> gi|297723921|ref|NP_001174324.1|








Os05g0292700 [Oryza








sativa Japonica Group]








> gi|68565732|sp|Q6END7.1|RK14_ORYNI








RecName:








Full = 50S ribosomal protein L14,








chloroplastic








> gi|148839610|sp|P0C438.1|RK14_ORYSA








RecName:








Full = 50S ribosomal protein L14,








chloroplastic








> gi|148839611|sp|P0C439.1|RK14_ORYSI








RecName: Full = 50S








ribosomal protein L14,








chloroplastic








> gi|148839612|sp|P0C440.1|RK14_ORYSJ








RecName: Full = 50S








ribosomal protein L14,








chloroplastic








> gi|12023|emb|CAA33932.1|








ribosomal protein L14 [Oryza








sativa Japonica Group]








> gi|20160833|dbj|BAB89773.1|








Chloroplast ribosomal protein








L14 [Oryza sativa Japonica








Group]








> gi|40253571|dbj|BAD05517.1|








ribosomal protein L14 [Oryza








sativa Japonica Group]








> gi|42795513|gb|AAS46080.1|








ribosomal protein L14 [Oryza








sativa Indica Group]








> gi|49615032|dbj|BAD26815.1|








ribosomal protein L14 [Oryza








nivara]








> gi|246367101|gb|ACS94712.1|








ribosomal protein L14








[Bambusa oldhamii]








> gi|255040295|gb|ACT99955.1|








ribosomal protein L14








[Dendrocalamus latiflorus]








> gi|255676212|dbj|BAH93052.1|








Os05g0292700 [Oryza sativa








Japonica Group]








> gi|307133922|gb|ADN32927.1|








ribosomal protein L14








[Phyllostachys nigra var. henonis]








> gi|309321653|gb|ADO65178.1|








ribosomal protein L14








[Acidosasa purpurea]








> gi|309321737|gb|ADO65261.1|








ribosomal protein L14








[Ferrocalamus rimosivaginus]








> gi|309321821|gb|ADO65344.1|








ribosomal protein L14








[Indocalamus longiauritus]








> gi|309321904|gb|ADO65426.1|








ribosomal protein L14








[Phyllostachys edulis]








> gi|309321989|gb|ADO65510.1|








ribosomal protein L14








[Bambusa emeiensis]








> gi|226644|prf||1603356BU








ribosomal protein L14







YP_00200522
ribosomal protein L14
0.98
2212
2614





[Brachypodium distachyon]








> gi|218546902|sp|B3TN86.1|RK14_BRADI








RecName: Full = 50S








ribosomal protein L14,








chloroplastic








> gi|193075591|gb|ACF08674.1|








ribosomal protein L14








[Brachypodium distachyon]







ABJ52167
ribosomal protein L14
0.98
2213
2615





[Phyllostachys edulis]







BAC10087
Chloroplast 50S ribosomal protein
0.98
2214
2616





L14 [Oryza sativa Japonica








Group]








> gi|50510231|dbj|BAD31429.1|








Chloroplast 50S ribosomal








protein L14 [Oryza sativa








Japonica Group]







YP_003587704
ribosomal protein L14
0.95
2215
2617





[Anomochloa marantoidea]








> gi|251765288|gb|ACT15442.1|








ribosomal protein L14








[Anomochloa marantoidea]







BAD33446
putative ribosomal protein L14
0.96
2216
2618





[Oryza sativa Japonica Group]








> gi|50726203|dbj|BAD33722.1|








putative ribosomal protein L14








[Oryza sativa Japonica Group]







AAT85078
putative 50S ribosomal protein
0.94
2217
2619





L14 [Oryza sativa Japonica








Group]








> gi|50878445|gb|AAT85219.1|








putative 50S ribosomal protein








L14 [Oryza sativa Japonica








Group]





Predicted
403-422
XP_002439358
hypothetical protein
1.00
2218
2620


siRNA


SORBIDRAFT_09g005070





60387


[Sorghum bicolor]








> gi|241944643|gb|EES17788.1|








hypothetical protein








SORBIDRAFT_09g005070








[Sorghum bicolor]







NP_001140641
hypothetical protein
0.88
2219
2621





LOC100272716 [Zea mays]








> gi|194700306|gb|ACF84237.1|








unknown [Zea mays]







ACG56678
tryptophan aminotransferase [Zea
0.88
2220
2622





mays]







NP_001151869
alliin lyase 2 [Zea mays]
0.79
2221
2623





> gi|195650459|gb|ACG44697.1|








alliin lyase 2 precursor [Zea








mays]






374-393
NP_001140641
hypothetical protein
1.00
2222
2624





LOC100272716 [Zea mays]








> gi|194700306|gb|ACF84237.1|








unknown [Zea mays]







ACG56678
tryptophan aminotransferase [Zea
1.00
2223
2625





mays]







XP_002439358
hypothetical protein
0.89
2224
2626





SORBIDRAFT_09g005070








[Sorghum bicolor]








> gi|241944643|gb|EES17788.1|








hypothetical protein








SORBIDRAFT_09g005070








[Sorghum bicolor]







NP_001151869
alliin lyase 2 [Zea mays]
0.77
2225
2627





> gi|195650459|gb|ACG44697.1|








alliin lyase 2 precursor [Zea








mays]






155-174
XP_002446287
hypothetical protein
1.00
2226
2628





SORBIDRAFT_06g013470








[Sorghum bicolor]








> gi|241937470|gb|EES10615.1|








hypothetical protein








SORBIDRAFT_06g013470








[Sorghum bicolor]







NP_001132422
hypothetical protein
0.89
2227
2629





LOC100193871 [Zea mays]








> gi|194694338|gb|ACF81253.1|








unknown [Zea mays]







ACN36503
unknown [Zea mays]
0.89
2228
2630




NP_001144520
hypothetical protein
0.89
2229
2631





LOC100277514 [Zea mays]








> gi|195643396|gb|ACG41166.1|








hypothetical protein [Zea mays]







ACN25444
unknown [Zea mays]
0.89
2230
2632




XP_002454376
hypothetical protein
0.83
2231
2633





SORBIDRAFT_04g029660








[Sorghum bicolor]








> gi|241934207|gb|EE507352.1|








hypothetical protein








SORBIDRAFT_04g029660








[Sorghum bicolor]






302-321
XP_002445712
hypothetical protein
1.00
2232
2634





SORBIDRAFT_07g024570








[Sorghum bicolor]








> gi|241942062|gb|EES15207.1|








hypothetical protein








SORBIDRAFT_07g024570








[Sorghum bicolor]







NP_001148095
auxin-independent growth
0.95
2233
2635





promoter [Zea mays]








> gi|195615748|gb|ACG29704.1|








auxin-independent growth








promoter [Zea mays]







BAD10226
putative auxin-independent
0.82
2234
2636





growth promoter [Oryza sativa








Japonica Group]








> gi|50725540|dbj|BAD33009.1|








putative auxin-independent








growth promoter [Oryza sativa








Japonica Group]







XP_002460613
hypothetical protein
0.71
2235
2637





SORBIDRAFT_02g031900








[Sorghum bicolor]








> gi|241923990|gb|EER97134.1|








hypothetical protein








SORBIDRAFT_02g031900








[Sorghum bicolor]






151-170
NP_001144386
hypothetical protein
1.00
2236
2638





LOC100277314 [Zea mays]








> gi|195641382|gb|ACG40159.1|








hypothetical protein [Zea mays]






781-800
NP_001151955
senescence-associated protein 15
1.00
2237
2639





[Zea mays]








> gi|195651331|gb|ACG45133.1|








senescence-associated protein








15 [Zea mays]






46-65
XP_002465563
hypothetical protein
1.00
2238
2640





SORBIDRAFT_01g041190








[Sorghum bicolor]








> gi|241919417|gb|EER92561.1|








hypothetical protein








SORBIDRAFT_01g041190








[Sorghum bicolor]







NP_001049540
Os03g0245700 [Oryza sativa
0.88
2239
2641





Japonica Group]








> gi|108707147|gb|ABF94942.1|








senescence-associated protein








15, putative, expressed [Oryza








sativa Japonica Group]








> gi|113548011|dbj|BAF11454.1|








Os03g0245700 [Oryza sativa








Japonica Group]








> gi|125543096|gb|EAY89235.1|








hypothetical protein OsI_10732








[Oryza sativa Indica Group]








> gi|125585589|gb|EAZ26253.1|








hypothetical protein OsJ_10120








[Oryza sativa Japonica Group]







BAJ85769
predicted protein [Hordeum
0.86
2240
2642





vulgare subsp. vulgare]








> gi|326514266|dbj|BAJ92283.1|








predicted protein [Hordeum








vulgare subsp. vulgare]







XP_002464724
hypothetical protein
0.76
2241
2643





SORBIDRAFT_01g025820








[Sorghum bicolor]








> gi|241918578|gb|EER91722.1|








hypothetical protein








SORBIDRAFT_01g025820








[Sorghum bicolor]







AAC34858
senescence-associated protein 15
0.70
2242
2644





[Hemerocallis hybrid cultivar]







EEC66585
hypothetical protein OsI_32794
0.74
2243






[Oryza sativa Indica Group]







EEE50594
hypothetical protein OsJ_30776
0.74
2244






[Oryza sativa Japonica Group]







NP_001064196
Os10g0158100 [Oryza sativa
0.73
2245
2645





Japonica Group]








> gi|255679225|dbj|BAF26110.2|








Os10g0158100 [Oryza sativa








Japonica Group]






400-419
NP_001149275
DNA polymerase epsilon subunit
1.00
2246
2646





3 [Zea mays]








> gi|195625970|gb|ACG34815.1|








DNA polymerase epsilon








subunit 3 [Zea mays]








> gi|195628668|gb|ACG36164.1|








DNA polymerase epsilon








subunit 3 [Zea mays]








> gi|223942345|gb|ACN25256.1|








unknown [Zea mays]







XP_002461994
hypothetical protein
0.71
2247
2647





SORBIDRAFT_02g012030








[Sorghum bicolor]








> gi|241925371|gb|EER98515.1|








hypothetical protein








SORBIDRAFT_02g012030








[Sorghum bicolor]






71-90
NP_001141681
hypothetical protein
1.00
2248
2648





LOC100273808 [Zea mays]








> gi|238908879|gb|ACF86850.2|








unknown [Zea mays]






106-125
XP_002468642
hypothetical protein
1.00
2249
2649





SORBIDRAFT_01g049490








[Sorghum bicolor]








> gi|241922496|gb|EER95640.1|








hypothetical protein








SORBIDRAFT_01g049490








[Sorghum bicolor]







ACG46521
F-box protein [Zea mays]
0.83
2250
2650



670-689
XP_002444258
hypothetical protein
1.00
2251
2651





SORBIDRAFT_07g019090








[Sorghum bicolor]








> gi|241940608|gb|EES13753.1|








hypothetical protein








SORBIDRAFT_07g019090








[Sorghum bicolor]







NP_001131520
hypothetical protein
0.95
2252
2652





LOC100192859 [Zea mays]








> gi|194691750|gb|ACF79959.1|








unknown [Zea mays]







ACG32608
peroxisomal coenzyme A
0.95
2253
2653





diphosphatase NUDT7 [Zea








mays]







EAZ06644
hypothetical protein OsI_28893
0.74
2254






[Oryza sativa Indica Group]








> gi|125561356|gb|EAZ06804.1|








hypothetical protein OsI_29048








[Oryza sativa Indica Group]







EAZ42551
hypothetical protein OsJ_27117
0.74
2255






[Oryza sativa Japonica Group]







EAZ06802
hypothetical protein OsI_29046
0.72
2256






[Oryza sativa Indica Group]







NP_001061674
Os08g0375900 [Oryza sativa
0.72
2257
2654





Japonica Group]








> gi|40253324|dbj|BAD05258.1|








putative phosphohydrolase








[Oryza sativa Japonica Group]








> gi|40253358|dbj|BAD05290.1|








putative phosphohydrolase








[Oryza sativa Japonica Group]








> gi|113623643|dbj|BAF23588.1|








Os08g0375900 [Oryza sativa








Japonica Group]







XP_002444259
hypothetical protein
0.71
2258
2655





SORBIDRAFT_07g019110








[Sorghum bicolor]








> gi|241940609|gb|EES13754.1|








hypothetical protein








SORBIDRAFT_07g019110








[Sorghum bicolor]






2195-2214
XP_002440313
hypothetical protein
1.00
2259
2656





SORBIDRAFT_09g029610








[Sorghum bicolor]








> gi|241945598|gb|EES18743.1|








hypothetical protein








SORBIDRAFT_09g029610








[Sorghum bicolor]







ACG34981
glucose-1-phosphate
0.97
2260
2657





adenylyltransferase large subunit








[Zea mays]








> gi|223948357|gb|ACN28262.1|








unknown [Zea mays]







NP_001056424
Os05g0580000 [Oryza sativa
0.93
2261
2658





Japonica Group]








> gi|51854319|gb|AAU10700.1|








putative glucose-1-phosphate








adenylyltransferase [Oryza sativa








Japonica Group]








> gi|125553462|gb|EAY99171.1|








hypothetical protein OsI_21129








[Oryza sativa Indica Group]








> gi|169244411|gb|ACA50479.1|








ADP-glucose








pyrophosphorylase large subunit








[Oryza sativa Japonica Group]








> gi|215704797|dbj|BAG94825.1|








unnamed protein product








[Oryza sativa Japonica Group]








> gi|222632685|gb|EEE64817.1|








hypothetical protein OsJ_19673








[Oryza sativa Japonica Group]








> gi|255676601|dbj|BAF18338.2|








Os05g0580000 [Oryza sativa








Japonica Group]








> gi|262344368|gb|ACY56044.1|








ADP-glucose








pyrophosphorylase large subunit








[Oryza sativa Japonica Group]








> gi|262344370|gb|ACY56045.1|








ADP-glucose








pyrophosphorylase large subunit








[Oryza sativa Japonica Group]








> gi|262344372|gb|ACY56046.1|








ADP-glucose








pyrophosphorylase large subunit








[Oryza sativa Japonica Group]








> gi|262344374|gb|ACY56047.1|








ADP-glucose








pyrophosphorylase large subunit








[Oryza sativa Japonica Group]








> gi|262344376|gb|ACY56048.1|








ADP-glucose








pyrophosphorylase large subunit








[Oryza sativa Japonica Group]








> gi|262344378|gb|ACY56049.1|








ADP-glucose








pyrophosphorylase large subunit








[Oryza sativa Japonica Group]








> gi|262344380|gb|ACY56050.1|








ADP-glucose








pyrophosphorylase large subunit








[Oryza sativa Indica Group]








> gi|262344382|gb|ACY56051.1|








ADP-glucose








pyrophosphorylase large subunit








[Oryza sativa Indica Group]








> gi|262344384|gb|ACY56052.1|








ADP-glucose








pyrophosphorylase large subunit








[Oryza sativa Indica Group]








> gi|262344386|gb|ACY56053.1|








ADP-glucose








pyrophosphorylase large subunit








[Oryza sativa Indica Group]








> gi|262344388|gb|ACY56054.1|








ADP-glucose








pyrophosphorylase large subunit








[Oryza sativa Indica Group]








> gi|262344390|gb|ACY56055.1|








ADP-glucose








pyrophosphorylase large subunit








[Oryza sativa Indica Group]








> gi|262344392|gb|ACY56056.1|








ADP-glucose








pyrophosphorylase large subunit








[Oryza sativa Indica Group]








> gi|262344394|gb|ACY56057.1|








ADP-glucose








pyrophosphorylase large subunit








[Oryza sativa Indica Group]







BAA23490
ADP glucose pyrophosphorylase
0.92
2262
2659





large subunit [Oryza sativa








Japonica Group]







AAD39597
10A19I.12 [Oryza sativa Japonica
0.93
2263
2660





Group]







P12299
RecName: Full = Glucose-1-
0.91
2264






phosphate adenylyltransferase








large subunit,








chloroplastic/amyloplastic;








AltName: Full = ADP-glucose








pyrophosphorylase; AltName:








Full = ADP-glucose synthase;








AltName: Full = AGPase S;








AltName: Full = Alpha-D-glucose-








1-phosphate adenyl transferase;








Flags: Precursor








> gi|995746|emb|CAA79980.1|








ADP-glucose pyrophosphorylase








large subunit [Triticum aestivum]








> gi|110729318|gb|ABG88200.1|








ADP-glucose








pyrophosphorylase large subunit








[Triticum aestivum]







P30524
RecName: Full = Glucose-1-
0.91
2265






phosphate adenylyltransferase








large subunit 1,








chloroplastic/amyloplastic;








AltName: Full = ADP-glucose








pyrophosphorylase; AltName:








Full = ADP-glucose synthase;








AltName: Full = AGPase S;








AltName: Full = Alpha-D-glucose-








1-phosphate adenyl transferase;








AltName: Full = BEPL; Flags:








Precursor








> gi|1279513|emb|CAA47626.1|








glucose-1-phosphate








adenylyltransferase [Hordeum








vulgare subsp. vulgare]








> gi|229610847|emb|CAX51355.1|








large subunit of ADP-glucose








pyrophosphorylase [Hordeum








vulgare subsp. vulgare]








> gi|326527375|dbj|BAK04629.1|








predicted protein [Hordeum








vulgare subsp. vulgare]








> gi|326528409|dbj|BAJ93393.1|








predicted protein [Hordeum








vulgare subsp. vulgare]








> gi|326528511|dbj|BAJ93437.1|








predicted protein [Hordeum








vulgare subsp. vulgare]







CAD98749
ADP-glucose pyrophosphorylase
0.89
2266
2661





large subunit [Triticum aestivum]







NP_001105717
glucose-1-phosphate
0.92
2267
2662





adenylyltransferase large subunit








2, chloroplastic/amyloplastic [Zea








mays]








>gi|707928|sp|p55234.1|GLG








L2_MAIZE RecName:








Full = Glucose-1-phosphate








adenylyltransferase large subunit








2, chloroplastic/amyloplastic;








AltName: Full = ADP-glucose








pyrophosphorylase; AltName:








AltName: Full = synthase








AltName: Full = AGPase S;








AltName: Full = Alpha-D-glucose-








1-phosphate adenyl transferase;








Flags: Precursor








>gi|558365|emb|CAA86227.1|








ADP-glucose pyrophosphorylase








[Zea mays]







1909370A
ADP glucose
0.87
2268



Predicted
154-171
XP_001545081
hypothetical protie BC1G_16418
1.00
2269
2663


siRNA


[Botryotinia fuckeliana B05.10 ]





60837


>gi|150854146|gb|EDN29338.1|








hypothetical protein








BCIG16418[Botryotinia fuckeliana








B05.10






629-646
XP_002441128
hypothetical protein
1.00
2270
2664





SORBIDRAFT_09g020930








[Sorghum bicolor]








>:g1|241946413|gb|EES19558.1|








hypothetical protein








SORBIDRAFT_09g020930








[Sorghum bicolor]







ACR35990
unknown [Zea mays]
0.91
2271
2665




NP_001152387
LOC100286027 [Zea mays]
0.92
2272
2666





>gi|195655783|gb|ACG47359.1|








secondary cell wall-related








glycosyltransferase family 8








[Zea mays]







BAJ85213
predicted protein [Hordeum
0.86
2273
2667





vulgare subsp. vulgare]








>gi|326515442|dbj|BAK03634.1|








predicted protein [Hordeum








vulgare subsp. vulgare]







AAS90653
putative glycogenin [Oryza sativa
0.84
2274
2668





Japonica Group]







EEC79261
hypothetical protein OsI_20036
0.84
2275






[Oryza sativa Indica Group]







EEE63788
hypothetical protein OsJ_18611
0.83
2276






[Oryza sativa Japonica Group]







NP—00104491
Os01g0880200 [Oryza sativa
0.84
2277
2669





Japonica Group]








>gi|56784626|dbj|BAD81673.1|








glycogenin-like protein [Oryza








sativa Japonica Group]








> gi|113534522|dbj|BAF06905.1|








Os01g0880200 [Oryza sativa








Japonica Group]







EEC71899
hypothetical protein OsI_04661
0.84
2278






[Oryza sativa Indica Group]







BAJ87677
predicted protein [Hordeum
0.82
2279
2670





vulgare subsp. vulgare]






687-704
EEC83357
hypothetical protein OsI_28763
1.00
2280






[Oryza sativa Indica Group]








> gi|222623167|gb|EEE57299.1|








hypothetical protein OsJ_07374








[Oryza sativa Japonica Group]







NP_001060995
Os08g0150800 [Oryza sativa
0.94
2281
2671





Japonica Group]








> gi|37573038|dbj|BAC98550.1|








putative tyrosyl-tRNA








synthetase [Oryza sativa Japonica








Group]








> gi|113622964|dbj|BAF22909.1|








Os08g0150800 [Oryza sativa








Japonica Group]








> gi|222639916|gb|EEE68048.1|








hypothetical protein OsJ_26046








[Oryza sativa Japonica Group]







ACF82652
unknown [Zea mays]
0.87
2282
2672




NP_001149217
LOC100282839 [Zea mays]
0.86
2283
2673





> gi|195625536|gb|ACG34598.1|








tyrosyl-tRNA synthetase [Zea








mays]







NP_001148699
tyrosyl-tRNA synthetase [Zea
0.83
2284
2674





mays]








> gi|195621482|gb|ACG32571.1|








tyrosyl-tRNA synthetase [Zea








mays]







BAK05692
predicted protein [Hordeum
0.83
2285
2675





vulgare subsp. vulgare]








> gi|326527699|dbj|BAK08124.1|








predicted protein [Hordeum








vulgare subsp. vulgare]








> gi|326533322|dbj|BAJ93633.1|








predicted protein [Hordeum








vulgare subsp. vulgare]







CAA71881
Tyrosyl-tRNA synthetase
0.69
2286
2676





[Nicotiana tabacum]







XP_002517485
tyrosyl-tRNA synthetase, putative
0.71
2287
2677





[Ricinus communis]








> gi|223543496|gb|EEF45027.1|








tyrosyl-tRNA synthetase,








putative [Ricinus communis]







XP_002274369
PREDICTED: hypothetical
0.71
2288
2678





protein [Vitis vinifera]








> gi|297742518|emb|CBI34667.3|








unnamed protein product [Vitis








vinifera]







CAN65657
hypothetical protein
0.71
2289
2679





VITISV_000951 [Vitis vinifera]






796-813
ACG35054
THO complex subunit 4 [Zea
1.00
2290
2680





mays]







NP_001146734
hypothetical protein
0.99
2291
2681





LOC100280336 [Zea mays]








> gi|194704900|gb|ACF86534.1|








unknown [Zea mays]








> gi|219888533|gb|ACL54641.1|








unknown [Zea mays]







XP_002438180
hypothetical protein
0.87
2292
2682





SORBIDRAFT_10g009240








[Sorghum bicolor]








> gi|241916403|gb|EER89547.1|








hypothetical protein








SORBIDRAFT_10g009240








[Sorghum bicolor]






310-327
NP_001168358
hypothetical protein
1.00
2293
2683





LOC100382126 [Zea mays]








> gi|223947741|gb|ACN27954.1|








unknown [Zea mays]







BAD54151
putative poly(A) polymerase
0.81
2294






[Oryza sativa Japonica Group]







BAJ95542
predicted protein [Hordeum
0.80
2295
2684





vulgare subsp. vulgare]







NP_001174846
Os06g0558700 [Oryza sativa
0.80
2296
2685





Japonica Group]








> gi|255677141|dbj|BAH93574.1|








Os06g0558700 [Oryza sativa








Japonica Group]







AAW68015
putative polynucleotide
0.80
2297
2686





adenylyltransferase [Oryza sativa








Indica Group]







EAZ37348
hypothetical protein OsJ_21686
0.78
2298






[Oryza sativa Japonica Group]







EAZ01335
hypothetical protein OsI_23369
0.78
2299






[Oryza sativa Indica Group]






576-593
XP_002466644
hypothetical protein
1.00
2300
2687





SORBIDRAFT_01g011560








[Sorghum bicolor]








> gi|241920498|gb|EER93642.1|








hypothetical protein








SORBIDRAFT_01g011560








[Sorghum bicolor]







NP_001169332
hypothetical protein
0.96
2301
2688





LOC100383199 [Zea mays]








> gi|224028751|gb|ACN33451.1|








unknown [Zea mays]







NP_001130678
hypothetical protein
0.95
2302
2689





LOC100191781 [Zea mays]








> gi|194688930|gb|ACF78549.1|








unknown [Zea mays]







EEC81302
hypothetical protein OsI_24438
0.87
2303






[Oryza sativa Indica Group]








> gi|222636212|gb|EEE66344.1|








hypothetical protein OsJ_22634








[Oryza sativa Japonica Group]







BAJ99110
predicted protein [Hordeum
0.86
2304
2690





vulgare subsp. vulgare]







BAJ86220
predicted protein [Hordeum
0.75
2305
2691





vulgare subsp. vulgare]







NP_001063757
Os09g0531900 [Oryza sativa
0.74
2306
2692





Japonica Group]








> gi|52075938|dbj|BAD46018.1|








glycosyl transferase family 8








protein-like [Oryza sativa








Japonica Group]








> gi|52077221|dbj|BAD46265.1|








glycosyl transferase family 8








protein-like [Oryza sativa








Japonica Group]








> gi|113631990|dbj|BAF25671.1|








Os09g0531900 [Oryza sativa








Japonica Group]








> gi|222641970|gb|EEE70102.1|








hypothetical protein OsJ_30110








[Oryza sativa Japonica Group]







EEC84934
hypothetical protein OsI_32147
0.74
2307






[Oryza sativa Indica Group]







XP_002462717
hypothetical protein
0.75
2308
2693





SORBIDRAFT_02g030820








[Sorghum bicolor]








> gi|241926094|gb|EER99238.1|








hypothetical protein








SORBIDRAFT_02g030820








[Sorghum bicolor]







CBI38820
unnamed protein product [Vitis
0.72
2309






vinifera]





Predicted
70-89
XP_002446795
hypothetical protein
1.00
2310
2694


siRNA


SORBIDRAFT_06g022830





61382


[Sorghum bicolor]








> gi|241937978|gb|EES11123.1|








hypothetical protein








SORBIDRAFT_06g022830








[Sorghum bicolor]







NP_001143685
hypothetical protein
0.86
2311
2695





LOC100276413 [Zea mays]








> gi|195624570|gb|ACG34115.1|








hypothetical protein [Zea mays]







ACN29321
unknown [Zea mays]
0.86
2312
2696




NP_001053334
Os04g0520900 [Oryza sativa
0.72
2313
2697





Japonica Group]








> gi|21741854|emb|CAD41444.1|








OSJNBa0019D11.15 [Oryza








sativa Japonica Group]








> gi|13564905|dbj|BAF15248.1|








Os04g0520900 [Oryza sativa








Japonica Group]








> gi|116310729|emb|CAH67525.1|








OSIGBa0131L05.6 [Oryza








sativa Indica Group]








> gi|125549050|gb|EAY94872.1|








hypothetical protein OsI_16672








[Oryza sativa Indica Group]








> gi|125591012|gb|EAZ31362.1|








hypothetical protein OsJ_15488








[Oryza sativa Japonica Group]








> gi|215697100|dbj|BAG91094.1|








unnamed protein product








[Oryza sativa Japonica Group]






682-701
NP_001143685
hypothetical protein
1.00
2314
2698





LOC100276413 [Zea mays]








> gi|195624570|gb|ACG34115.1|








hypothetical protein [Zea mays]







ACN29321
unknown [Zea mays]
0.95
2315
2699




XP_002446795
hypothetical protein
0.90
2316
2700





SORBIDRAFT_06g022830








[Sorghum bicolor]








> gi|241937978|gb|EES11123.1|








hypothetical protein








SORBIDRAFT_06g022830








[Sorghum bicolor]







NP_001053334
Os04g0520900 [Oryza sativa
0.70
2317
2701





Japonica Group]








> gi|21741854|emb|CAD41444.1|








OSJNBa0019D11.15 [Oryza








sativa Japonica Group]








> gi|113564905|dbj|BAF15248.1|








Os04g0520900 [Oryza sativa








Japonica Group]








> gi|116310729|emb|CAH67525.1|








OSIGBa0131L05.6 [Oryza








sativa Indica Group]








> gi|125549050|gb|EAY94872.1|








hypothetical protein OsI_16672








[Oryza sativa Indica Group]








> gi|125591012|gb|EAZ31362.1|








hypothetical protein OsJ_15488








[Oryza sativa Japonica Group]








> gi|215697100|dbj|BAG91094.1|








unnamed protein product








[Oryza sativa Japonica Group]





Predicted
237-254
XP_002459573
hypothetical protein
1.00
2318
2702


siRNA


SORBIDRAFT_02g006770





60188


[Sorghum bicolor]








> gi|241922950|gb|EER96094.1|








hypothetical protein








SORBIDRAFT_02g006770








[Sorghum bicolor]







NP_001150031
nucleic acid binding protein [Zea
0.98
2319
2703





mays]








> gi|194699002|gb|ACF83585.1|








unknown [Zea mays]








> gi|195636208|gb|ACG37572.1|








nucleic acid binding protein








[Zea mays]







NP_001140438
hypothetical protein
0.96
2320
2704





LOC100272497 [Zea mays]








> gi|194699514|gb|ACF83841.1|








unknown [Zea mays]







NP_001059216
Os07g0227400 [Oryza sativa
0.91
2321
2705





Japonica Group]








> gi|24060154|dbj|BAC21599.1|








KH domain-like protein [Oryza








sativa Japonica Group]








> gi|113610752|dbj|BAF21130.1|








Os07g0227400 [Oryza sativa








Japonica Group]








> gi|215697533|dbj|BAG91527.1|








unnamed protein product








[Oryza sativa Japonica Group]







EAZ39172
hypothetical protein OsJ_23597
0.91
2322






[Oryza sativa Japonica Group]








> gi|218199326|gb|EEC81753.1|








hypothetical protein OsI_25419








[Oryza sativa Indica Group]







NP_001148920
nucleic acid binding protein [Zea
0.85
2323
2706





mays]








> gi|195623320|gb|ACG33490.1|








nucleic acid binding protein








[Zea mays]







XP_002466215
hypothetical protein
0.85
2324
2707





SORBIDRAFT_01g003680








[Sorghum bicolor]








> gi|241920069|gb|EER93213.1|








hypothetical protein








SORBIDRAFT_01g003680








[Sorghum bicolor]







NP_001051685
Os03g0815700 [Oryza sativa
0.86
2325
2708





Japonica Group]








> gi|75226290|sp|Q75GR5.1|SPIN1_ORYSJ








RecName: Full = KH








domain-containing protein SPIN1;








AltName: Full = SPL11-interacting








protein 1








> gi|37718879|gb|AAR01750.1|








expressed protein [Oryza sativa








Japonica Group]








> gi|108711745|gb|ABF99540.1|








KH domain-containing protein,








putative, expressed [Oryza sativa








Japonica Group]








> gi|113550156|dbj|BAF13599.1|








Os03g0815700 [Oryza sativa








Japonica Group]








> gi|215694514|dbj|BAG89507.1|








unnamed protein product








[Oryza sativa Japonica Group]








> gi|218193980|gb|EEC76407.1|








hypothetical protein OsI_14057








[Oryza sativa Indica Group]








> gi|222626037|gb|EEE60169.1|








hypothetical protein OsJ_13097








[Oryza sativa Japonica Group]







NP_001130116
hypothetical protein
0.84
2326
2709





LOC100191210 [Zea mays]








> gi|194688334|gb|ACF78251.1|








unknown [Zea mays]







BAJ96236
predicted protein [Hordeum
0.83
2327
2710





vulgare subsp. vulgare]






362-379
XP_002448500
hypothetical protein
1.00
2328
2711





SORBIDRAFT_06g028030








[Sorghum bicolor]








> gi|241939683|gb|EES12828.1|








hypothetical protein








SORBIDRAFT_06g028030








[Sorghum bicolor]







NP_001148728
protein phosphatase 2C [Zea
0.93
2329
2712





mays]








> gi|195621678|gb|ACG32669.1|








protein phosphatase 2C [Zea








mays]








> gi|238007398|gb|ACR34734.1|








unknown [Zea mays]







NP_001053823
Os04g0609600 [Oryza sativa
0.91
2330
2713





Japonica Group]








> gi|122240832|sp|Q0JAA0.1|P2C44_ORYSJ








RecName:








Full = Probable protein








phosphatase 2C 44;








Short = OsPP2C44








> gi|113565394|dbj|BAF15737.1|








Os04g0609600 [Oryza sativa








Japonica Group]







5
[Oryza sativa Japonica Group]







EEC71777
hypothetical protein OsI_04389
0.80
2339






[Oryza sativa Indica Group]







BAJ99773
predicted protein [Hordeum
0.78
2340
2721





vulgare subsp. vulgare]







XP_002458747
hypothetical protein
0.78
2341
2722





SORBIDRAFT_03g039530








[Sorghum bicolor]








> gi|241930722|gb|EES03867.1|








hypothetical protein








SORBIDRAFT_03g039530








[Sorghum bicolor]







AAC04576
putative high-pI laccase [Oryza
0.79
2342
2723





sativa Japonica Group]






725-742
CAE03557
OSJNBa0085I10.2 [Oryza sativa
1.00
2343
2724





Japonica Group]








> gi|90265077|emb|CAH67750.1|








H0702G05.9 [Oryza sativa








Indica Group]







NP_001053823
Os04g0609600 [Oryza sativa
1.00
2344
2725





Japonica Group]








> gi|122240832|sp|Q0JAA0.1|P2C44_ORYSJ








RecName:








Full = Probable protein








phosphatase 2C 44;








Short = OsPP2C44








> gi|113565394|dbj|BAF15737.1|








Os04g0609600 [Oryza sativa








Japonica Group]








> gi|218195541|gb|EEC77968.1|








hypothetical protein OsI_17335








[Oryza sativa Indica Group]








> gi|222629521|gb|EEE61653.1|








hypothetical protein OsJ_16105








[Oryza sativa Japonica Group]







XP_002448500
hypothetical protein
0.91
2345
2726





SORBIDRAFT_06g028030








[Sorghum bicolor]








> gi|241939683|gb|EES12828.1|








hypothetical protein








SORBIDRAFT_06g028030








[Sorghum bicolor]







NP_001148728
protein phosphatase 2C [Zea
0.90
2346
2727





mays]








> gi|195621678|gb|ACG32669.1|








protein phosphatase 2C [Zea








mays]








> gi|238007398|gb|ACR34734.1|








unknown [Zea mays]







CAE03658
OSJNBa0060NO3.23 [Oryza
0.75
2347
2728





sativa Japonica Group]







XP_002274944
PREDICTED: hypothetical
0.74
2348
2729





protein isoform 1 [Vitis vinifera]








> gi|297745124|emb|CB138963.3|








unnamed protein product [Vitis








vinifera]







XP_002514493
protein phosphatase 2c, putative
0.76
2349
2730





[Ricinus communis]








> gi|223546392|gb|EEF47893.1|








protein phosphatase 2c, putative








[Ricinus communis]







XP_002274981
PREDICTED: hypothetical
0.71
2350
2731





protein isoform 2 [Vitis vinifera]






879-896
NP_001054756
Os05g0168700 [Oryza sativa
1.00
2351
2732





Japonica Group]








> gi|53982148|gb|AAV25244.1|








putative phosphate translocator








[Oryza sativa Japonica Group]








> gi|113578307|dbj|BAF16670.1|








Os05g0168700 [Oryza sativa








Japonica Group]








> gi|215686739|dbj|BAG89589.1|








unnamed protein product








[Oryza sativa Japonica Group]








> gi|218196167|gb|EEC78594.1|








hypothetical protein OsI_18612








[Oryza sativa Indica Group]








> gi|222630341|gb|EEE62473.1|








hypothetical protein OsJ_17270








[Oryza sativa Japonica Group]







BAB41206
putative glucose-6-
0.99
2352
2733





phosphate/phosphate-tranlocat or








[Oryza sativa (japonica cultivar-








group)]







ACN32013
unknown [Zea mays]
0.96
2353
2734




XP_002440680
hypothetical protein
0.96
2354
2735





SORBIDRAFT_09g005010








[Sorghum bicolor]








> gi|241945965|gb|EES19110.1|








hypothetical protein








SORBIDRAFT_09g005010








[Sorghum bicolor]







NP_001142411
hypothetical protein
0.95
2355
2736





LOC100274586 [Zea mays]








> gi|194707946|gb|ACF88057.1|








unknown [Zea mays]








> gi|194708688|gb|ACF88428.1|








unknown [Zea mays]








> gi|195620476|gb|ACG32068.1|








integral membrane protein like








[Zea mays]







BAK03308
predicted protein [Hordeum
0.95
2356
2737





vulgare subsp. vulgare]







NP_001148556
integral membrane protein like
0.87
2357
2738





[Zea mays]








> gi|195620390|gb|ACG32025.1|








integral membrane protein like








[Zea mays]







NP_001042121
Os01g0167500 [Oryza sativa
0.92
2358
2739





Japonica Group]








> gi|13486667|dbj|BAB39904.1|








P0028E10.8 [Oryza sativa








Japonica Group]








> gi|15528768|dbj|BAB64810.1|








putative glucose-6-








phosphate/phosphate-tranlocator








[Oryza sativa Japonica Group]








> gi|20804811|dbj|BAB92494.1|








putative glucose-6-








phosphate/phosphate-tranlocator








[Oryza sativa Japonica Group]








> gi|113531652|dbj|BAF04035.1|








Os01g0167500 [Oryza sativa








Japonica Group]








> gi|215767361|dbj|BAG99589.1|








unnamed protein product








[Oryza sativa Japonica Group]








> gi|218187579|gb|EEC70006.1|








hypothetical protein OsI_00550








[Oryza sativa Indica Group]








> gi|222617800|gb|EEE53932.1|








hypothetical protein OsJ_00515








[Oryza sativa Japonica Group]







NP_001142171
hypothetical protein
0.93
2359
2740





LOC100274338 [Zea mays]








> gi|194707458|gb|ACF87813.1|








unknown [Zea mays]







ACN34739
unknown [Zea mays]
0.92
2360
2741



130-147
XP_002448782
hypothetical protein
1.00
2361
2742





SORBIDRAFT_06g033075








[Sorghum bicolor]








> gi|241939965|gb|EES13110.1|








hypothetical protein








SORBIDRAFT_06g033075








[Sorghum bicolor]







NP_001151057
LOC100284690 [Zea mays]
0.92
2362
2743





> gi|195643970|gb|ACG41453.1|








ATP binding protein [Zea








mays]







EAZ32429
hypothetical protein OsJ_16639
0.82
2363






[Oryza sativa Japonica Group]







NP_001054279
Os04g0679200 [Oryza sativa
0.82
2364
2744





Japonica Group]








> gi|38344039|emb|CAE05726.2|








OSJNBb0017I01.6 [Oryza








sativa Japonica Group]








> gi|113565850|dbj|BAF16193.1|








Os04g0679200 [Oryza sativa








Japonica Group]








> gi|215712397|dbj|BAG94524.1|








unnamed protein product








[Oryza sativa Japonica Group]








> gi|215740866|dbj|BAG97022.1|








unnamed protein product








[Oryza sativa JaponicaGroup]







CAJ86254
H0801D08.12 [Oryza sativa
0.82
2365
2745





Indica Group]








> gi|125550244|gb|EAY96066.1|








hypothetical protein OsI_17939








[Oryza sativa Indica Group]







BAJ93433
predicted protein [Hordeum
0.80
2366
2746





vulgare subsp. vulgare]






99-116
XP_002452000
hypothetical protein
1.00
2367
2747





SORBIDRAFT_04g014390








[Sorghum bicolor]








> gi|241931831|gb|EES04976.1|








hypothetical protein








SORBIDRAFT_04g014390








[Sorghum bicolor]







ACR36123
unknown [Zea mays]
0.93
2368
2748




NP_001145206
hypothetical protein
0.92
2369
2749





LOC100278461 [Zea mays]








> gi|195652631|gb|ACG45783.1|








hypothetical protein [Zea mays]







NP_001046716
Os02g0329300 [Oryza sativa
0.86
2370
2750





Japonica Group]








> gi|46389946|dbj|BAD15798.1|








unknown protein [Oryza sativa








Japonica Group]








> gi|113536247|dbj|BAF08630.1|








Os02g0329300 [Oryza sativa








Japonica Group]








> gi|222622747|gb|EEE56879.1|








hypothetical protein OsJ_06518








[Oryza sativa Japonica Group]







EEC73063
hypothetical protein OsI_07020
0.85
2371






[Oryza sativa Indica Group]







BAK07073
predicted protein [Hordeum
0.81
2372
2751





vulgare subsp. vulgare]






736-753
ACN28855
unknown [Zea mays]
1.00
2373
2752




NP_001105875
putative laccase [Zea mays]
0.99
2374
2753





> gi|84618781|emb|CAJ30500.1|








putative laccase [Zea mays]







NP_001146658
hypothetical protein
0.93
2375
2754





LOC100280258 [Zea mays]








> gi|219888209|gb|ACL54479.1|








unknown [Zea mays]







XP_002458746
hypothetical protein
0.93
2376
2755





SORBIDRAFT_03g039520








[Sorghum bicolor]








> gi|241930721|gb|EES03866.1|








hypothetical protein








SORBIDRAFT_03g039520








[Sorghum bicolor]







NP_001044772
Os01g0842400 [Oryza sativa
0.81
2377
2756





Japonica Group]








> gi|75321217|sp|Q5N9X2.1|LAC4_ORYSJ








RecName:








Full = Laccase-4; AltName:








Full = Benzenediol:oxygen








oxidoreductase 4; AltName:








Full = Diphenol oxidase 4;








AltName: Full = Urishiol oxidase








4; Flags: Precursor








> gi|56784239|dbj|BAD81734.1|








putative laccase LAC5-6








[Oryza sativa Japonica Group]








> gi|113534303|dbj|BAF06686.1|








Os01g0842400 [Oryza sativa








Japonica Group]








> gi|215697155|dbj|BAG91149.1|








unnamed protein product








[Oryza sativa Japonica Group]







EAZ14115
hypothetical protein OsJ_04039
0.81
2378






[Oryza sativa Japonica Group]







EEC71777
hypothetical protein OsI_04389
0.80
2379






[Oryza sativa Indica Group]







BAJ99773
predicted protein [Hordeum
0.78
2380
2757





vulgare subsp. vulgare]







XP_002458747
hypothetical protein
0.78
2381
2758





SORBIDRAFT_03g039530








[Sorghum bicolor]








> gi|241930722|gb|EES03867.1|








hypothetical protein








SORBIDRAFT_03g039530








[Sorghum bicolor]







AAC04576
putative high-pI laccase [Oryza
0.79
2382
2759





sativa Japonica Group]






0-17
NP_001132686
hypothetical protein
1.00
2383
2760





LOC100194164 [Zea mays]








> gi|194695090|gb|ACF81629.1|








unknown [Zea mays]







XP_002457397
hypothetical protein
0.81
2384
2761





SORBIDRAFT_03g006690








[Sorghum bicolor]








> gi|241929372|gb|EES02517.1|








hypothetical protein








SORBIDRAFT_03g006690








[Sorghum bicolor]






1000-1017
NP_001136742
hypothetical protein
1.00
2385
2762





LOC100216883 [Zea mays]








> gi|194696868|gb|ACF82518.1|








unknown [Zea mays]








> gi|195648274|gb|ACG43605.1|








monoglyceride lipase [Zea








mays]







XP_002450081
hypothetical protein
0.80
2386
2763





SORBIDRAFT_05g000200








[Sorghum bicolor]








> gi|241935924|gb|EES09069.1|








hypothetical protein








SORBIDRAFT_05g000200








[Sorghum bicolor]







XP_002442631
hypothetical protein
0.80
2387
2764





SORBIDRAFT_08g000200








[Sorghum bicolor]








> gi|241943324|gb|EES16469.1|








hypothetical protein








SORBIDRAFT_08g000200








[Sorghum bicolor]






296-313
XP_002440680
hypothetical protein
1.00
2388
2765





SORBIDRAFT_09g005010








[Sorghum bicolor]








> gi|241945965|gb|EES19110.1|








hypothetical protein








SORBIDRAFT_09g005010








[Sorghum bicolor]







ACN32013
unknown [Zea mays]
0.99
2389
2766




NP_001142411
hypothetical protein
0.98
2390
2767





LOC100274586 [Zea mays]








> gi|194707946|gb|ACF88057.1|








unknown [Zea mays]








> gi|194708688|gb|ACF88428.1|








unknown [Zea mays]








> gi|195620476|gb|ACG32068.1|








integral membrane protein like








[Zea mays]







NP_001054756
Os05g0168700 [Oryza sativa
0.96
2391
2768





Japonica Group]








> gi|53982148|gb|AAV25244.1|








putative phosphate translocator








[Oryza sativa Japonica Group]








> gi|113578307|dbj|BAF16670.1|








Os05g0168700 [Oryza sativa








Japonica Group]








> gi|215686739|dbj|BAG89589.1|








unnamed protein product








[Oryza sativa Japonica Group]








> gi|218196167|gb|EEC78594.1|








hypothetical protein OsI_18612








[Oryza sativa Indica Group]








> gi|222630341|gb|EEE62473.1|








hypothetical protein OsJ_17270








[Oryza sativa Japonica Group]







BAB41206
putative glucose-6-
0.95
2392
2769





phosphate/phosphate-tranlocat or








[Oryza sativa (japonica cultivar-








group)]







BAK03308
predicted protein [Hordeum
0.94
2393
2770





vulgare subsp. vulgare]







NP_001148556
integral membrane protein like
0.90
2394
2771





[Zea mays]








> gi|195620390|gb|ACG32025.1|








integral membrane protein like








[Zea mays]







NP_001042121
Os01g0167500 [Oryza sativa
0.92
2395
2772





Japonica Group]








> gi|13486667|dbj|BAB39904.1|








P0028E10.8 [Oryza sativa








Japonica Group]








> gi|15528768|dbj|BAB64810.1|








putative glucose-6-








phosphate/phosphate-tranlocator








[Oryza sativa Japonica Group]








> gi|20804811|dbj|BAB92494.1|








putative glucose-6-








phosphate/phosphate-tranlocator








[Oryza sativa Japonica Group]








> gi|113531652|dbj|BAF04035.1|








Os01g0167500 [Oryza sativa








Japonica Group]








> gi|215767361|dbj|BAG99589.1|








unnamed protein product








[Oryza sativa Japonica Group]








> gi|218187579|gb|EEC70006.1|








hypothetical protein OsI_00550








[Oryza sativa Indica Group]








> gi|222617800|gb|EEE53932.1|








hypothetical protein OsJ_00515








[Oryza sativa Japonica Group]







NP_001142171
hypothetical protein
0.92
2396
2773





LOC100274338 [Zea mays]








> gi|194707458|gb|ACF87813.1|








unknown [Zea mays]







ACN34739
unknown [Zea mays]
0.91
2397
2774



1638-1655
NP_001183153
hypothetical protein
1.00
2398
2775





LOC100501523 [Zea mays]








> gi|238009674|gb|ACR35872.1|








unknown [Zea mays]






1133-1150
NP_001146658
hypothetical protein
1.00
2399
2776





LOC100280258 [Zea mays]








> gi|219888209|gb|ACL54479.1|








unknown [Zea mays]







NP_001105875
putative laccase [Zea mays]
0.95
2400
2777





> gi|84618781|emb|CAJ30500.1|








putative laccase [Zea mays]







ACN28855
unknown [Zea mays]
0.92
2401
2778




XP_002458746
hypothetical protein
0.91
2402
2779





SORBIDRAFT_03g039520








[Sorghum bicolor]








> gi|241930721|gb|EES03866.1|








hypothetical protein








SORBIDRAFT_03g039520








[Sorghum bicolor]







NP_001044772
Os01g0842400 [Oryza sativa
0.80
2403
2780





Japonica Group]








> gi|75321217|sp|Q5N9X2.1|LAC4_ORYSJ








RecName:








Full = Laccase-4; AltName:








Full = Benzenediol:oxygen








oxidoreductase 4; AltName:








Full = Diphenol oxidase 4;








AltName: Full = Urishiol oxidase








4; Flags: Precursor








> gi|56784239|dbj|BAD81734.1|








putative laccase LAC5-6








[Oryza sativa Japonica Group]








> gi|113534303|dbj|BAF06686.1|








Os01g0842400 [Oryza sativa








Japonica Group]








> gi|215697155|dbj|BAG91149.1|








unnamed protein product








[Oryza sativa Japonica Group]







EAZ14115
hypothetical protein OsJ_04039
0.80
2404






[Oryza sativa Japonica Group]







EEC71777
hypothetical protein OsI_04389
0.80
2405






[Oryza sativa Indica Group]







BAJ99773
predicted protein [Hordeum
0.78
2406
2781





vulgare subsp. vulgare]







AAC04576
putative high-pI laccase [Oryza
0.78
2407
2782





sativa Japonica Group]







XP_002458747
hypothetical protein
0.77
2408
2783





SORBIDRAFT_03g039530








[Sorghum bicolor]








> gi|241930722|gb|EES03867.1|








hypothetical protein








SORBIDRAFT_03g039530








[Sorghum bicolor]






574-591
NP_001053823
Os04g0609600 [Oryza sativa
1.00
2409
2784





Japonica Group]








> gi|122240832|sp|Q0JAA0.1|P2C44_ORYSJ








RecName:








Full = Probable protein








phosphatase 2C 44;








Short = OsPP2C44








> gi|113565394|dbj|BAF15737.1|








Os04g0609600 [Oryza sativa








Japonica Group]








> gi|218195541|gb|EEC77968.1|








hypothetical protein OsI_17335








[Oryza sativa Indica Group]








> gi|222629521|gb|EEE61653.1|








hypothetical protein OsJ_16105








[Oryza sativa Japonica Group]







CAE03557
OSJNBa0085I10.2 [Oryza sativa
0.99
2410
2785





Japonica Group]








> gi|90265077|emb|CAH67750.1|








H0702G05.9 [Oryza sativa








Indica Group]







XP_002448500
hypothetical protein
0.91
2411
2786





SORBIDRAFT_06g028030








[Sorghum bicolor]








> gi|241939683|gb|EES12828.1|








hypothetical protein








SORBIDRAFT_06g028030








[Sorghum bicolor]







NP_001148728
protein phosphatase 2C [Zea
0.90
2412
2787





mays]








> gi|195621678|gb|ACG32669.1|








protein phosphatase 2C [Zea








mays]








> gi|238007398|gb|ACR34734.1|








unknown [Zea mays]







CAE03658
OSJNBa0060N03.23 [Oryza
0.74
2413
2788





sativa Japonica Group]







XP_002274944
PREDICTED: hypothetical
0.73
2414
2789





protein isoform 1 [Vitis vinifera]








> gi|297745124|emb|CBI38963.3|








unnamed protein product [Vitis








vinifera]







XP_002514493
protein phosphatase 2c, putative
0.75
2415
2790





[Ricinus communis]








> gi|223546392|gb|EEF47893.1|








protein phosphatase 2c, putative








[Ricinus communis]







XP_002274981
PREDICTED: hypothetical
0.70
2416
2791





protein isoform 2 [Vitis vinifera]






1034-1051
NP_001170532
hypothetical protein
1.00
2417
2792





LOC100384546 [Zea mays]








> gi|238005884|gb|ACR33977.1|








unknown [Zea mays]







XP_002438672
hypothetical protein
0.88
2418
2793





SORBIDRAFT_10g024080








[Sorghum bicolor]








> gi|241916895|gb|EER90039.1|








hypothetical protein








SORBIDRAFT_10g024080








[Sorghum bicolor]







EAZ01659
hypothetical protein OsI_23694
0.72
2419






[Oryza sativa Indica Group]







EAZ37629
hypothetical protein OsJ_21963
0.72
2420






[Oryza sativa Japonica Group]







BAD35523
unknown protein [Oryza sativa
0.72
2421






Japonica Group]








> gi|51090952|dbj|BAD35555.1|








unknown protein [Oryza sativa








Japonica Group]






1728-1745
AAW66346
YZ1 [Zea luxurians]
1.00
2422
2794




AAW66344
YZ1 [Zea mays subsp. mexicana]
0.99
2423
2795




AAW66348
YZ1 [Zea mays subsp.
0.99
2424
2796





parviglumis]







AAM22634
YZ1 [Zea mays]
0.99
2425
2797




AAM21160
YZ1 [Zea mays]
0.98
2426
2798




ABB29302
YZ1 [Zea mays]
0.91
2427
2799





> gi|78172241|gb|ABB29304.1|








YZ1 [Zea mays]







NP_001043656
Os01g0633400 [Oryza sativa
0.71
2428
2800





Japonica Group]








> gi|55296003|dbj|BAD68894.1|








putative YZ1 [Oryza sativa








Japonica Group]








> gi|113533187|dbj|BAF05570.1|








Os01g0633400 [Oryza sativa








Japonica Group]








> gi|215766352|dbj|BAG98580.1|








unnamed protein product








[Oryza sativa Japonica Group]







XP_002456014
hypothetical protein
0.77
2429
2801





SORBIDRAFT_03g028870








[Sorghum bicolor]








> gi|241927989|gb|EES01134.1|








hypothetical protein








SORBIDRAFT_03g028870








[Sorghum bicolor]







EAY75077
hypothetical protein OsI_02971
0.71
2430






[Oryza sativa Indica Group]





Predicted
689-710
XP_002443139
hypothetical protein
1.00
2431
2802


siRNA


SORBIDRAFT_08g011100





59659


[Sorghum bicolor]








> gi|241943832|gb|EES16977.1|








hypothetical protein








SORBIDRAFT_08g011100








[Sorghum bicolor]







NP_001169596
hypothetical protein
0.94
2432
2803





LOC100383477 [Zea mays]








> gi|224030279|gb|ACN34215.1|








unknown [Zea mays]







NP_001066586
Os12g0285600 [Oryza sativa
0.83
2433
2804





Japonica Group]








> gi|77554686|gb|ABA97482.1|








Oxysterol-binding protein,








expressed [Oryza sativa Japonica








Group]








> gi|113649093|dbj|BAF29605.1|








Os12g0285600 [Oryza sativa








Japonica Group]








> gi|215697261|dbj|BAG91255.1|








unnamed protein product








[Oryza sativa Japonica Group]








> gi|222616918|gb|EEE53050.1|








hypothetical protein OsJ_35782








[Oryza sativa Japonica Group]







EEC69105
hypothetical protein OsI_38013
0.81
2434






[Oryza sativa Indica Group]







CBI16832
unnamed protein product [Vitis
0.71
2435






vinifera]







XP_002282089
PREDICTED: similar to
0.72
2436
2805





oxysterol-binding family protein








[Vitis vinifera]





Table 7: Provided are the target Genes of siRNAs Associated with Increased NUE (Table 3) along with their GenBank Accession numbers and sequence identifiers (SEQ ID NO:).


“bind” = binding;


“pos” = position;


“hom” = homologue;


“p.p.” = polypeptide;


“p.n.” = polynucleotide.













TABLE 8







Target genes of down regulated siRNA sequences associated with NUE


(Table 4)


















p.p.
p.n.



Mir
Hom.


SEQ
SEQ


Mir
Bind.
NCBI


ID
ID


name
Pos.
Accession
Annotation/Organism
Identity
NO:
NO:





Predicted
61-79
EEE65047
hypothetical protein OsJ_20044 [Oryza
1.00
2806



siRNA


sativa Japonica Group]





59123










NP_001056727
Os06g0136600 [Oryza sativa Japonica
0.97
2807
3082





Group] > gi|55296986|dbj|BAD68461.1|








putative enolase [Oryza sativa Japonica








Group] > gi|55297212|dbj|BAD68886.1|








putative enolase [Oryza sativa Japonica








Group]








> gi|113594767|dbj|BAF18641.1|








Os06g0136600 [Oryza sativa Japonica








Group] > gi|218197519|gb|EEC79946.1|








hypothetical protein OsI_21538 [Oryza








sativa Indica Group]







NP_001105896
enolase 1 [Zea mays]
0.92
2808
3083





> gi|119355|sp|P26301.1|ENO1_MAIZE








RecName: Full = Enolase 1; AltName:








Full = 2-phospho-D-glycerate hydro-lyase








1; AltName: Full = 2-phosphoglycerate








dehydratase 1








> gi|22273|emb|CAA39454.1|enolase








[Zea mays]







ACL53816
unknown [Zea mays]
0.92
2809
3084




ACN26258
unknown [Zea mays]
0.88
2810
3085




ACG31732
enolase [Zea mays]
0.87
2811
3086




XP_002322420
predicted protein [Populus trichocarpa]
0.88
2812
3087





> gi|118484871|gb|ABK94302.1|








unknown [Populus trichocarpa]








> gi|222869416|gb|EEF06547.1|








predicted protein [Populus trichocarpa]







NP_001105371
enolase 2 [Zea mays]
0.87
2813
3088





> gi|1169528|sp|P42895.1|ENO2_MAIZE








RecName: Full = Enolase 2; AltName:








Full = 2-phospho-D-glycerate hydro-lyase








2; AltName: Full = 2-phosphoglycerate








dehydratase 2








> gi|602253|gb|AAD04187.1|enolase








[Zea mays]








> gi|37222051|gb|AAQ17040.2|pollen








2-phosphoglycerate dehydrogenase 2








precursor [Cynodon dactylon]







ACF06525
enolase [Elaeis guineensis]
0.87
2814
3089




BAJ90134
predicted protein [Hordeum vulgare subsp.
0.87
2815
3090





vulgare]






45-63
NP_001143947
hypothetical protein LOC100276760 [Zea
1.00
2816
3091





mays] > gi|195630673|gb|ACG36643.1|








hypothetical protein [Zea mays]







ACR36446
unknown [Zea mays]
0.97
2817
3092




NP_001143988
hypothetical protein LOC100276806 [Zea
0.79
2818
3093





mays] > gi|194707932|gb|ACF88050.1|








unknown [Zea mays]








> gi|195619872|gb|ACG31766.1|








hypothetical protein [Zea mays]








> gi|195634969|gb|ACG36953.1|








hypothetical protein [Zea mays]







XP_002450179
hypothetical protein
0.81
2819
3094





SORBIDRAFT_05g001600 [Sorghum








bicolor] > gi|241936022|gb|EES09167.1|








hypothetical protein








SORBIDRAFT_05g001600 [Sorghum








bicolor]







NP_001065630
Os11g0127700 [Oryza sativa Japonica
0.70
2820
3095





Group] > gi|77548485|gb|ABA91282.1|








expressed protein [Oryza sativa Japonica








Group]








> gi|113644334|dbj|BAF27475.1|








Os11g0127700 [Oryza sativa Japonica








Group] > gi|125576047|gb|EAZ17269.1|








hypothetical protein OsJ_32788 [Oryza








sativa Japonica Group]







EAY79782
hypothetical protein OsI_34938 [Oryza
0.70
2821






sativa Indica Group]






2433-2451
XP_002466271
hypothetical protein
1.00
2822
3096





SORBIDRAFT_01g004840 [Sorghum








bicolor]








> gi|241920125|gb|EER93269.1|








hypothetical protein








SORBIDRAFT_01g004840 [Sorghum








bicolor]







NP_001145769
hypothetical protein LOC100279276 [Zea
0.98
2823
3097





mays] > gi|219884363|gb|ACL52556.1|








unknown [Zea mays]







NP_001151633
vacuolar protein sorting 35 [Zea mays]
0.98
2824
3098





> gi|195648240|gb|ACG43588.1|








vacuolar protein sorting 35 [Zea mays]







EAY92214
hypothetical protein OsI_13933 [Oryza
0.95
2825






sativa Indica Group]







NP_001051593
Os03g0801600 [Oryza sativa Japonica
0.95
2826
3099





Group] > gi|29150373|gb|AAO72382.1|








putative vacuolar protein sorting-








associated protein [Oryza sativa Japonica








Group] > gi|108711598|gb|ABF99393.1|








vacuolar protein sorting-associated protein








35 family protein, putative, expressed








[Oryza sativa Japonica Group]








> gi|113550064|dbj|BAF13507.1|








Os03g0801600 [Oryza sativa Japonica








Group] > gi|125588278|gb|EAZ28942.1|








hypothetical protein OsJ_12986 [Oryza








sativa Japonica Group]








> gi|215704706|dbj|BAG94334.1|








unnamed protein product [Oryza sativa








Japonica Group]







BAJ89592
predicted protein [Hordeum vulgare subsp.
0.95
2827
3100





vulgare]








> gi|326488479|dbj|BAJ93908.1|








predicted protein [Hordeum vulgare subsp.








vulgare]







XP_002272227
PREDICTED: hypothetical protein isoform
0.82
2828
3101





1 [Vitis vinifera]







XP_002272268
PREDICTED: hypothetical protein isoform
0.81
2829
3102





2 [Vitis vinifera]







BAJ53108
JHL20J20.15 [Jatropha curcas]
0.79
2830





CBI17331
unnamed protein product [Vitis vinifera]
0.81
2831




20-38
XP_002442446
hypothetical protein
1.00
2832
3103





SORBIDRAFT_08g020140 [Sorghum








bicolor] > gi|241943139|gb|EES16284.1|








hypothetical protein








SORBIDRAFT_08g020140 [Sorghum








bicolor]







ACF80272
unknown [Zea mays]
0.84
2833
3104




NP_001141063
hypothetical protein LOC100273144 [Zea
0.84
2834
3105





mays] > gi|194702462|gb|ACF85315.1|








unknown [Zea mays]







ACG44331
hypothetical protein [Zea mays]
0.84
2835
3106




BAJ89075
predicted protein [Hordeum vulgare subsp.
0.70
2836
3107





vulgare]








> gi|326533254|dbj|BAJ93599.1|








predicted protein [Hordeum vulgare subsp.








vulgare]





Predicted
713-731
CAI30078
glycosyltransferase [Sorghum bicolor]
1.00
2837
3108


siRNA








57685










NP_001105165
glycosyltransferase [Zea mays]
0.96
2838
3109





> gi|56409864|emb|CAI30080.1|








glycosyltransferase [Zea mays]







CAI30079
glycosyltransferase [Saccharum
0.95
2839
3110





officinarum]







BAJ96053
predicted protein [Hordeum vulgare subsp.
0.81
2840
3111





vulgare]







NP_001064930
Os10g0492200 [Oryza sativa Japonica
0.77
2841
3112





Group]








> gi|10140717|gb|AAG13551.1|AC023240_24








unknown protein [Oryza sativa








Japonica Group]








> gi|31432816|gb|AAP54403.1|








glycosyltransferase, putative, expressed








[Oryza sativa Japonica Group]








> gi|113639539|dbj|BAF26844.1|








Os10g0492200 [Oryza sativa Japonica








Group]








> gi|215766456|dbj|BAG98764.1|








unnamed protein product [Oryza sativa








Japonica Group]








> gi|222613059|gb|EEE51191.1|








hypothetical protein OsJ_31998 [Oryza








sativa Japonica Group]







EEC67226
hypothetical protein OsI_34143 [Oryza
0.76
2842






sativa Indica Group]







CCA61105
TaGT61_1 [Triticum aestivum]
0.70
2843
3113




BAK04539
predicted protein [Hordeum vulgare subsp.
0.71
2844
3114





vulgare]







NP_001046724
Os02g0331200 [Oryza sativa Japonica
0.71
2845
3115





Group] > gi|46390170|dbj|BAD15603.1|








putative HGA1 [Oryza sativa Japonica








Group] > gi|46390974|dbj|BAD16509.1|








putative HGA1 [Oryza sativa Japonica








Group]








> gi|113536255|dbj|BAF08638.1|








Os02g0331200 [Oryza sativa Japonica








Group]








> gi|215712340|dbj|BAG94467.1|








unnamed protein product [Oryza sativa








Japonica Group]








> gi|215737036|dbj|BAG95965.1|








unnamed protein product [Oryza sativa








Japonica Group]






344-362
YP_588428
hypothetical protein ZeamMp184 [Zea
1.00
2846






mays subsp. mays]








> gi|40795041|gb|AAR91085.1|








hypothetical protein [Zea mays]








> gi|93116163|gb|ABE98794.1|








hypothetical protein [Zea mays subsp.








mays] > gi|102579667|gb|ABF70947.1|








hypothetical protein [Zea mays subsp.








mays]







ABE98707
hypothetical protein [Zea mays subsp.
0.90
2847






mays]






1201-1219
CAI30079
glycosyltransferase [Saccharum
1.00
2848
3116





officinarum]







NP_001105165
glycosyltransferase [Zea mays]
0.96
2849
3117





> gi|56409864|emb|CAI30080.1|








glycosyltransferase [Zea mays]







CAI30078
glycosyltransferase [Sorghum bicolor]
0.97
2850
3118




BAJ96053
predicted protein [Hordeum vulgare subsp.
0.83
2851
3119





vulgare]







NP_001064930
Os10g0492200 [Oryza sativa Japonica
0.77
2852
3120





Group]








> gi|10140717|gb|AAG13551.1|AC023240_24








unknown protein [Oryza sativa








Japonica Group]








> gi|31432816|gb|AAP54403.1|








glycosyltransferase, putative, expressed








[Oryza sativa Japonica Group]








> gi|113639539|dbj|BAF26844.1|








Os10g0492200 [Oryza sativa Japonica








Group]








> gi|215766456|dbj|BAG98764.1|








unnamed protein product [Oryza sativa








Japonica Group]








> gi|222613059|gb|EEE51191.1|








hypothetical protein OsJ_31998 [Oryza








sativa Japonica Group]







EEC67226
hypothetical protein OsI_34143 [Oryza
0.78
2853






sativa Indica Group]







BAK04539
predicted protein [Hordeum vulgare subsp.
0.71
2854
3121





vulgare]







CCA61105
TaGT61_1 [Triticum aestivum]
0.70
2855
3122




BAD15602
putative HGA1 [Oryza sativa Japonica
0.71
2856






Group] > gi|46390975|dbj|BAD16510.1|








putative HGA1 [Oryza sativa Japonica








Group]








> gi|215686668|dbj|BAG88921.1|








unnamed protein product [Oryza sativa








Japonica Group]







NP_001046724
Os02g0331200 [Oryza sativa Japonica
0.71
2857
3123





Group] > gi|46390170|dbj|BAD15603.1|








putative HGA1 [Oryza sativa Japonica








Group] > gi|46390974|dbj|BAD16509.1|








putative HGA1 [Oryza sativa Japonica








Group]








> gi|113536255|dbj|BAF08638.1|








Os02g0331200 [Oryza sativa Japonica








Group]








> gi|215712340|dbj|BAG94467.1|








unnamed protein product [Oryza sativa








Japonica Group]








> gi|215737036|dbj|BAG95965.1|








unnamed protein product [Oryza sativa








Japonica Group]





Predicted
Jan-
XP_002440494
hypothetical protein
1.00
2858
3124


siRNA
19

SORBIDRAFT_09g001910 [Sorghum





59993


bicolor] > gi|241945779|gb|EES18924.1|








hypothetical protein








SORBIDRAFT_09g001910 [Sorghum








bicolor]







ACG27529
splicing factor, arginine/serine-rich 7 [Zea
0.87
2859
3125





mays] > gi|223973611|gb|ACN30993.1|








unknown [Zea mays]







NP_001049771
Os03g0285900 [Oryza sativa Japonica
0.74
2860
3126





Group] > gi|108707560|gb|ABF95355.1|








RNA recognition motif family protein,








expressed [Oryza sativa Japonica Group]








> gi|113548242|dbj|BAF11685.1|








Os03g0285900 [Oryza sativa Japonica








Group] > gi|222624706|gb|EEE58838.1|








hypothetical protein OsJ_10413 [Oryza








sativa Japonica Group]







EEC75006
hypothetical protein OsI_11073 [Oryza
0.70
2861






sativa Indica Group]







NP_001132009
hypothetical protein LOC100193414 [Zea
0.72
2862
3127





mays] > gi|194693184|gb|ACF80676.1|








unknown [Zea mays]







AAY84873
alternative splicing regulator [Triticum
0.72
2863
3128





aestivum]






14-32
XP_002458875
hypothetical protein
1.00
2864
3129





SORBIDRAFT_03g041990 [Sorghum








bicolor] > gi|241930850|gb|EES03995.1|








hypothetical protein








SORBIDRAFT_03g041990 [Sorghum








bicolor]







NP_001142991
hypothetical protein LOC100275452 [Zea
0.92
2865
3130





mays] > gi|195612624|gb|ACG28142.1|








hypothetical protein [Zea mays]







NP_001145171
hypothetical protein LOC100278407 [Zea
0.91
2866
3131





mays] > gi|195652179|gb|ACG45557.1|








hypothetical protein [Zea mays]







ACF83626
unknown [Zea mays]
0.92
2867
3132




EAZ14403
hypothetical protein OsJ_04323 [Oryza
0.89
2868






sativa Japonica Group]







EAY76740
hypothetical protein OsI_04696 [Oryza
0.90
2869






sativa Indica Group]







BAK00430
predicted protein [Hordeum vulgare subsp.
0.84
2870
3133





vulgare]






2352-2370
NP_001067313
Os12g0623900 [Oryza sativa Japonica
1.00
2871
3134





Group] > gi|77556631|gb|ABA99427.1|








5-methyltetrahydropteroyltriglutamate-








homocysteine methyltransferase, putative,








expressed [Oryza sativa Japonica Group]








> gi|108862991|gb|ABG22094.1|5-








methyltetrahydropteroyltriglutamate-








homocysteine methyltransferase, putative,








expressed [Oryza sativa Japonica Group]








> gi|113649820|dbj|BAF30332.1|








Os12g0623900 [Oryza sativa Japonica








Group] > gi|125537469|gb|EAY83957.1|








hypothetical protein OsI_39179 [Oryza








sativa Indica Group]








> gi|125580128|gb|EAZ21274.1|








hypothetical protein OsJ_36926 [Oryza








sativa Japonica Group]







ABG22095
5-methyltetrahydropteroyltriglutamate-
0.99
2872






homocysteine methyltransferase, putative,








expressed [Oryza sativa Japonica Group]








> gi|108862993|gb|ABG22096.1|5-








methyltetrahydropteroyltriglutamate-








homocysteine methyltransferase, putative,








expressed [Oryza sativa Japonica Group]







NP_001067314
Os12g0624000 [Oryza sativa Japonica
0.98
2873
3135





Group] > gi|77556632|gb|ABA99428.1|








5-methyltetrahydropteroyltriglutamate-








homocysteine methyltransferase, putative,








expressed [Oryza sativa Japonica Group]








> gi|77556633|gb|ABA99429.1|5-








methyltetrahydropteroyltriglutamate-








homocysteine methyltransferase, putative,








expressed [Oryza sativa Japonica Group]








> gi|113649821|dbj|BAF30333.1|








Os12g0624000 [Oryza sativa Japonica








Group]








> gi|215695374|dbj|BAG90565.1|








unnamed protein product [Oryza sativa








Japonica Group]








> gi|218187282|gb|EEC69709.1|








hypothetical protein OsI_39180 [Oryza








sativa Indica Group]








> gi|222617511|gb|EEE53643.1|








hypothetical protein OsJ_36927 [Oryza








sativa Japonica Group]







ACN28399
unknown [Zea mays]
0.95
2874
3136




AAL33589
methionine synthase [Zea mays]
0.94
2875
3137




ACL54117
unknown [Zea mays]
0.94
2876
3138




NP_001152513
LOC100286153 [Zea mays]
0.94
2877
3139





> gi|195657041|gb|ACG47988.1|5-








methyltetrahydropteroyltriglutamate--








homocysteine methyltransferase [Zea








mays]







CAJ01714
methionine synthase 2 enzyme [Hordeum
0.94
2878
3140





vulgare subsp. vulgare]








> gi|326512178|dbj|BAJ96070.1|








predicted protein [Hordeum vulgare subsp.








vulgare]







BAJ89541
predicted protein [Hordeum vulgare subsp.
0.94
2879
3141





vulgare]







BAJ88127
predicted protein [Hordeum vulgare subsp.
0.94
2880
3142





vulgare]






121-139
ACL52492
unknown [Zea mays]
1.00
2881
3143




XP_002465926
hypothetical protein
0.94
2882
3144





SORBIDRAFT_01g048350 [Sorghum








bicolor]








> gi|241919780|gb|EER92924.1|








hypothetical protein








SORBIDRAFT_01g048350 [Sorghum








bicolor]







NP_001048855
Os03g0130500 [Oryza sativa Japonica
0.80
2883
3145





Group] > gi|108706005|gb|ABF93800.1|








EF hand family protein, expressed [Oryza








sativa Japonica Group]








> gi|113547326|dbj|BAF10769.1|








Os03g0130500 [Oryza sativa Japonica








Group]







EAZ25463
hypothetical protein OsJ_09285 [Oryza
0.79
2884






sativa Japonica Group]







BAJ91192
predicted protein [Hordeum vulgare subsp.
0.80
2885
3146





vulgare]






49-67
ACV84253
LOX5 [Sorghum bicolor]
1.00
2886
3147




XP_002446554
hypothetical protein
1.00
2887
3148





SORBIDRAFT_06g018040 [Sorghum








bicolor] > gi|241937737|gb|EES10882.1|








hypothetical protein








SORBIDRAFT_06g018040 [Sorghum








bicolor]







ACL81190
tasselseed 1 [Zea mays]
0.95
2888
3149




NP_001105979
tassel seed1 [Zea mays]
0.95
2889






> gi|84626293|gb|ABC59691.1|








lipoxygenase [Zea mays]







ACL81191
tasselseed 1b [Zea mays]
0.91
2890
3150




NP_001105978
lipoxygenase7 [Zea mays]
0.90
2891
3151





> gi|84626291|gb|ABC59690.1|








lipoxygenase [Zea mays]







Q7XV13
RecName: Full = Putative lipoxygenase 5
0.86
2892






> gi|38344820|emb|CAD40882.2|








OSJNBa0064H22.1 [Oryza sativa Japonica








Group]








> gi|116310177|emb|CAH67189.1|








OSIGBa0152K17.1 [Oryza sativa Indica








Group]







BAJ94611
predicted protein [Hordeum vulgare subsp.
0.85
2893
3152





vulgare]








> gi|326511025|dbj|BAJ91860.1|








predicted protein [Hordeum vulgare subsp.








vulgare]







CAI84707
lipoxygenase-like protein [Hordeum
0.85
2894
3153





vulgare subsp. vulgare]







BAK03423
predicted protein [Hordeum vulgare subsp.
0.85
2895
3154





vulgare]






117-135
NP_001169757
hypothetical protein LOC100383639 [Zea
1.00
2896
3155





mays] > gi|224031489|gb|ACN34820.1|








unknown [Zea mays]








> gi|238006168|gb|ACR34119.1|








unknown [Zea mays]







ACG49031
ubiquinone biosynthesis methyltransferase
0.99
2897
3156





COQ5 [Zea mays]







NP_001151844
ubiquinone biosynthesis methyltransferase
0.93
2898
3157





COQ5 [Zea mays]








> gi|195650199|gb|ACG44567.1|








ubiquinone biosynthesis methyltransferase








COQ5 [Zea mays]







NP_001045568
Os01g0976600 [Oryza sativa Japonica
0.87
2899
3158





Group]








> gi|75320942|sp|Q5JNC0.1|COQ5_ORYSJ








RecName: Full = 2-methoxy-6-








polyprenyl-1,4-benzoquinol methylase,








mitochondrial; AltName: Full = Ubiquinone








biosynthesis methyltransferase COQ5;








Flags: Precursor








> gi|57899522|dbj|BAD87036.1|








putative ubiquinone [Oryza sativa Japonica








Group]








> gi|113535099|dbj|BAF07482.1|








Os01g0976600 [Oryza sativa Japonica








Group]








> gi|215706368|dbj|BAG93224.1|








unnamed protein product [Oryza sativa








Japonica Group]








> gi|222619975|gb|EEE56107.1|








hypothetical protein OsJ_04965 [Oryza








sativa Japonica Group]







EAY77437
hypothetical protein OsI_05431 [Oryza
0.87
2900






sativa Indica Group]







BAJ89795
predicted protein [Hordeum vulgare subsp.
0.78
2901
3159





vulgare]







XP_002457083
hypothetical protein
0.72
2902
3160





SORBIDRAFT_03g000990 [Sorghum








bicolor] > gi|241929058|gb|EES02203.1|








hypothetical protein








SORBIDRAFT_03g000990 [Sorghum








bicolor]







XP_002324457
predicted protein [Populus trichocarpa]
0.70
2903
3161





> gi|222865891|gb|EEF03022.1|








predicted protein [Populus trichocarpa]






656-674
XP_002444158
hypothetical protein
1.00
2904
3162





SORBIDRAFT_07g010240 [Sorghum








bicolor] > gi|241940508|gb|EES13653.1|








hypothetical protein








SORBIDRAFT_07g010240 [Sorghum








bicolor]







NP_001168336
hypothetical protein LOC100382104 [Zea
0.85
2905
3163





mays] > gi|223947549|gb|ACN27858.1|








unknown [Zea mays]







EAY97519
hypothetical protein OsI_19447 [Oryza
0.80
2906






sativa Indica Group]








> gi|222631101|gb|EEE63233.1|








hypothetical protein OsJ_18043 [Oryza








sativa Japonica Group]







BAJ96779
predicted protein [Hordeum vulgare subsp.
0.78
2907
3164





vulgare]







BAK02037
predicted protein [Hordeum vulgare subsp.
0.78
2908
3165





vulgare]







BAJ97459
predicted protein [Hordeum vulgare subsp.
0.78
2909
3166





vulgare]







AAV44115
unknown protein [Oryza sativa Japonica
0.78
2910






Group]






668-686
NP_001150708
zinc finger, C2H2 type family protein [Zea
1.00
2911
3167





mays] > gi|195641252|gb|ACG40094.1|








zinc finger, C2H2 type family protein [Zea








mays]







XP_002460014
hypothetical protein
0.87
2912
3168





SORBIDRAFT_02g020840 [Sorghum








bicolor]








> gi|241923391|gb|EER96535.1|








hypothetical protein








SORBIDRAFT_02g020840 [Sorghum








bicolor]







NP_001150040
zinc finger, C2H2 type family protein [Zea
0.78
2913
3169





mays] > gi|195636264|gb|ACG37600.1|








zinc finger, C2H2 type family protein [Zea








mays]







BAK04496
predicted protein [Hordeum vulgare subsp.
0.70
2914
3170





vulgare]






524-542
NP_001183648
hypothetical protein LOC100502242 [Zea
1.00
2915
3171





mays] > gi|238013654|gb|ACR37862.1|








unknown [Zea mays]






660-678
NP_001150040
zinc finger, C2H2 type family protein [Zea
1.00
2916
3172





mays] > gi|195636264|gb|ACG37600.1|








zinc finger, C2H2 type family protein [Zea








mays]







XP_002460014
hypothetical protein
0.78
2917
3173





SORBIDRAFT_02g020840 [Sorghum








bicolor]








> gi|241923391|gb|EER96535.1|








hypothetical protein








SORBIDRAFT_02g020840 [Sorghum








bicolor]







NP_001150708
zinc finger, C2H2 type family protein [Zea
0.76
2918
3174





mays] > gi|195641252|gb|ACG40094.1|








zinc finger, C2H2 type family protein [Zea








mays]






433-451
NP_001140599
hypothetical protein LOC100272670 [Zea
1.00
2919
3175





mays] > gi|194700138|gb|ACF84153.1|








unknown [Zea mays]






277-295
XP_002468199
hypothetical protein
1.00
2920
3176





SORBIDRAFT_01g041550 [Sorghum








bicolor]








> gi|241922053|gb|EER95197.1|








hypothetical protein








SORBIDRAFT_01g041550 [Sorghum








bicolor]







NP_001140579
hypothetical protein LOC100272649 [Zea
0.88
2921
3177





mays] > gi|194700056|gb|ACF84112.1|








unknown [Zea mays]







ACG35337
purple acid phosphatase 1 [Zea mays]
0.84
2922
3178




NP_001049500
Os03g0238600 [Oryza sativa Japonica
0.78
2923
3179





Group] > gi|108707073|gb|ABF94868.1|








purple acid phosphatase 1, putative,








expressed [Oryza sativa Japonica Group]








> gi|113547971|dbj|BAF11414.1|








Os03g0238600 [Oryza sativa Japonica








Group] > gi|125543049|gb|EAY89188.1|








hypothetical protein OsI_10684 [Oryza








sativa Indica Group]








> gi|125585546|gb|EAZ26210.1|








hypothetical protein OsJ_10077 [Oryza








sativa Japonica Group]








> gi|215697901|dbj|BAG92094.1|








unnamed protein product [Oryza sativa








Japonica Group]








> gi|215737711|dbj|BAG96841.1|








unnamed protein product [Oryza sativa








Japonica Group]








> gi|215740770|dbj|BAG96926.1|








unnamed protein product [Oryza sativa








Japonica Group]








> gi|215767489|dbj|BAG99717.1|








unnamed protein product [Oryza sativa








Japonica Group]







NP_001151094
LOC100284727 [Zea mays]
0.75
2924
3180





> gi|195644254|gb|ACG41595.1|purple








acid phosphatase 1 [Zea mays]







ACF83217
unknown [Zea mays]
0.74
2925
3181




ACN25848
unknown [Zea mays]
0.74
2926
3182




ACL54336
unknown [Zea mays]
0.74
2927
3183




BAI78301
purple acid phosphatase [Triticum
0.72
2928
3184





aestivum]







BAK06221
predicted protein [Hordeum vulgare subsp.
0.73
2929
3185





vulgare]








> gi|326500986|dbj|BAJ98724.1|








predicted protein [Hordeum vulgare subsp.








vulgare]








> gi|326530618|dbj|BAK01107.1|








predicted protein [Hordeum vulgare subsp.








vulgare]






121-139
XP_002465926
hypothetical protein
1.00
2930
3186





SORBIDRAFT_01g048350 [Sorghum








bicolor]








> gi|241919780|gb|EER92924.1|








hypothetical protein








SORBIDRAFT_01g048350 [Sorghum








bicolor]







ACL52492
unknown [Zea mays]
0.94
2931
3187




NP_001048855
Os03g0130500 [Oryza sativa Japonica
0.82
2932
3188





Group] > gi|108706005|gb|ABF93800.1|








EF hand family protein, expressed [Oryza








sativa Japonica Group]








> gi|113547326|dbj|BAF10769.1|








Os03g0130500 [Oryza sativa Japonica








Group]







EAZ25463
hypothetical protein OsJ_09285 [Oryza
0.80
2933






sativa Japonica Group]







BAJ91192
predicted protein [Hordeum vulgare subsp.
0.81
2934
3189





vulgare]






2507-2525
XP_002454851
hypothetical protein
1.00
2935
3190





SORBIDRAFT_04g038540 [Sorghum








bicolor] > gi|241934682|gb|EES07827.1|








hypothetical protein








SORBIDRAFT_04g038540 [Sorghum








bicolor]







NP_001183894
hypothetical protein LOC100502487 [Zea
0.90
2936
3191





mays] > gi|238015298|gb|ACR38684.1|








unknown [Zea mays]







BAJ86117
predicted protein [Hordeum vulgare subsp.
0.76
2937
3192





vulgare]








> gi|326521078|dbj|BAJ96742.1|








predicted protein [Hordeum vulgare subsp.








vulgare]







NP_001050813
Os03g0657100 [Oryza sativa Japonica
0.70
2938
3193





Group] > gi|108710182|gb|ABF97977.1|








U-box domain containing protein,








expressed [Oryza sativa Japonica Group]








> gi|113549284|dbj|BAF12727.1|








Os03g0657100 [Oryza sativa Japonica








Group] > gi|218193430|gb|EEC75857.1|








hypothetical protein OsI_12864 [Oryza








sativa Indica Group]







AAP50990
unknown protein [Oryza sativa Japonica
0.70
2939
3194





Group]






746-764
NP_001140297
hypothetical protein LOC100272342 [Zea
1.00
2940
3195





mays] > gi|194698890|gb|ACF83529.1|








unknown [Zea mays]






49-67
NP_001105978
lipoxygenase7 [Zea mays]
1.00
2941
3196





> gi|84626291|gb|ABC59690.1|








lipoxygenase [Zea mays]







ACL81191
tasselseed 1b [Zea mays]
0.98
2942
3197




XP_002446554
hypothetical protein
0.91
2943
3198





SORBIDRAFT_06g018040 [Sorghum








bicolor] > gi|241937737|gb|EES10882.1|








hypothetical protein








SORBIDRAFT_06g018040 [Sorghum








bicolor]







ACV84253
LOX5 [Sorghum bicolor]
0.90
2944
3199




NP_001105979
tassel seed1 [Zea mays]
0.91
2945
3200





> gi|84626293|gb|ABC59691.1|








lipoxygenase [Zea mays]







ACL81190
tasselseed 1 [Zea mays]
0.90
2946
3201




Q7XV13
RecName: Full = Putative lipoxygenase 5
0.83
2947






> gi|38344820|emb|CAD40882.2|








OSJNBa0064H22.1 [Oryza sativa Japonica








Group]








> gi|116310177|emb|CAH67189.1|








OSIGBa0152K17.1 [Oryza sativa Indica








Group]







CAI84707
lipoxygenase-like protein [Hordeum
0.82
2948
3202





vulgare subsp. vulgare]







BAJ94611
predicted protein [Hordeum vulgare subsp.
0.82
2949
3203





vulgare]








> gi|326511025|dbj|BAJ91860.1|








predicted protein [Hordeum vulgare subsp.








vulgare]







BAK03423
predicted protein [Hordeum vulgare subsp.
0.82
2950
3204





vulgare]





Predicted
256-274
XP_002461947
hypothetical protein
1.00
2951
3205


siRNA


SORBIDRAFT_02g011010 [Sorghum





60012


bicolor]








> gi|241925324|gb|EER98468.1|








hypothetical protein








SORBIDRAFT_02g011010 [Sorghum








bicolor]






948-966
XP_002457764
hypothetical protein
1.00
2952
3206





SORBIDRAFT_03g013140 [Sorghum








bicolor] > gi|241929739|gb|EES02884.1|








hypothetical protein








SORBIDRAFT_03g013140 [Sorghum








bicolor]







ACF87582
unknown [Zea mays]
0.93
2953
3207




NP_001105753
Pti1 protein [Zea mays]
0.92
2954
3208





> gi|49188602|gb|AAT57904.1|putative








PTI1-like kinase [Zea mays]







AAT57905
putative PTI1-like kinase [Zea mays]
0.92
2955
3209




AAT57906
putative PTI1-like kinase [Zea mays]
0.93
2956
3210




EAY73772
hypothetical protein OsI_01646 [Oryza
0.81
2957






sativa Indica Group]







NP_001042895
Os01g0323100 [Oryza sativa Japonica
0.81
2958
3211





Group] > gi|12328582|dbj|BAB21241.1|








putative Pto kinase interactor 1 [Oryza








sativa Japonica Group]








> gi|29027802|dbj|BAC65877.1|








putative Pto kinase interactor 1 [Oryza








sativa Japonica Group]








> gi|113532426|dbj|BAF04809.1|








Os01g0323100 [Oryza sativa Japonica








Group] > gi|125570160|gb|EAZ11675.1|








hypothetical protein OsJ_01536 [Oryza








sativa Japonica Group]








> gi|215694472|dbj|BAG89427.1|








unnamed protein product [Oryza sativa








Japonica Group]






1207-1225
XP_002451738
hypothetical protein
1.00
2959
3212





SORBIDRAFT_04g006950 [Sorghum








bicolor] > gi|241931569|gb|EES04714.1|








hypothetical protein








SORBIDRAFT_04g006950 [Sorghum








bicolor]







ACG38564
cerebral protein 1 [Zea mays]
0.96
2960
3213





> gi|238015424|gb|ACR38747.1|








unknown [Zea mays]







NP_001047303
Os02g0593900 [Oryza sativa Japonica
0.70
2961
3214





Group]








> gi|113536834|dbj|BAF09217.1|








Os02g0593900 [Oryza sativa Japonica








Group] > gi|125540112|gb|EAY86507.1|








hypothetical protein OsI_07887 [Oryza








sativa Indica Group]








> gi|125582717|gb|EAZ23648.1|








hypothetical protein OsJ_07349 [Oryza








sativa Japonica Group]






67-85
XP_002449762
hypothetical protein
1.00
2962
3215





SORBIDRAFT_05g022780 [Sorghum








bicolor] > gi|241935605|gb|EES08750.1|








hypothetical protein








SORBIDRAFT_05g022780 [Sorghum








bicolor]







ABA94447
ETO1-like protein 1, putative, expressed
0.93
2963
3216





[Oryza sativa Japonica Group]








> gi|125577639|gb|EAZ18861.1|








hypothetical protein OsJ_34400 [Oryza








sativa Japonica Group]







EAY81449
hypothetical protein OsI_36620 [Oryza
0.93
2964






sativa Indica Group]







NP_001068166
Os11g0585900 [Oryza sativa Japonica
0.90
2965
3217





Group]








> gi|113645388|dbj|BAF28529.1|








Os11g0585900 [Oryza sativa Japonica








Group]







NP_001146335
hypothetical protein LOC100279911 [Zea
0.91
2966
3218





mays] > gi|219886675|gb|ACL53712.1|








unknown [Zea mays]







CBI25039
unnamed protein product [Vitis vinifera]
0.73
2967





XP_002280519
PREDICTED: hypothetical protein [Vitis
0.72
2968
3219





vinifera]







AAZ08351
ethylene overproducer-like 1 [Solanum
0.71
2969
3220





lycopersicum]







ABB46489
ethylene-overproducer1-like protein
0.71
2970
3221





[Solanum lycopersicum]





Predicted
358-381
XP_002466013
hypothetical protein
1.00
2971
3222


siRNA


SORBIDRAFT_01g050070 [Sorghum





55081


bicolor]








> gi|241919867|gb|EER93011.1|








hypothetical protein








SORBIDRAFT_01g050070 [Sorghum








bicolor]







NP_001132709
hypothetical protein LOC100194192 [Zea
0.77
2972
3223





mays] > gi|194695168|gb|ACF81668.1|








unknown [Zea mays]







NP_001148998
xyloglucan endotransglucosylase/hydrolase
0.76
2973
3224





protein 32 [Zea mays]








> gi|195623856|gb|ACG33758.1|








xyloglucan endotransglucosylase/hydrolase








protein 32 precursor [Zea mays]





Predicted
364-382
XP_002439498
hypothetical protein
1.00
2974
3225


siRNA


SORBIDRAFT_09g008130 [Sorghum





59032


bicolor] > gi|241944783|gb|EES17928.1|








hypothetical protein








SORBIDRAFT_09g008130 [Sorghum








bicolor]







NP_001141460
hypothetical protein LOC100273570 [Zea
0.88
2975
3226





mays] > gi|194704658|gb|ACF86413.1|








unknown [Zea mays]








> gi|195620084|gb|ACG31872.1|triose








phosphate/phosphate translocator [Zea








mays] > gi|195625418|gb|ACG34539.1|








triose phosphate/phosphate translocator








[Zea mays]







ACG32515
triose phosphate/phosphate translocator
0.88
2976
3227





[Zea mays]







NP_001055001
Os05g0241200 [Oryza sativa Japonica
0.75
2977
3228





Group] > gi|53980843|gb|AAV24764.1|








putative phosphate translocator [Oryza








sativa Japonica Group]








> gi|113578552|dbj|BAF16915.1|








Os05g0241200 [Oryza sativa Japonica








Group] > gi|125551487|gb|EAY97196.1|








hypothetical protein OsI_19118 [Oryza








sativa Indica Group]








> gi|215765430|dbj|BAG87127.1|








unnamed protein product [Oryza sativa








Japonica Group]







BAJ94007
predicted protein [Hordeum vulgare subsp.
0.73
2978
3229





vulgare]






225-243
NP_001168807
hypothetical protein LOC100382608 [Zea
1.00
2979
3230





mays] > gi|223973113|gb|ACN30744.1|








unknown [Zea mays]





Predicted
227-245
XP_002447337
hypothetical protein
1.00
2980
3231


siRNA


SORBIDRAFT_06g033160 [Sorghum





58721


bicolor] > gi|241938520|gb|EES11665.1|








hypothetical protein








SORBIDRAFT_06g033160 [Sorghum








bicolor]







NP_001142056
hypothetical protein LOC100274212 [Zea
0.96
2981
3232





mays] > gi|194706940|gb|ACF87554.1|








unknown [Zea mays]








> gi|223947485|gb|ACN27826.1|








unknown [Zea mays]







ACG45259
hypothetical protein [Zea mays]
0.96
2982
3233




EEC78262
hypothetical protein OsI_17948 [Oryza
0.86
2983






sativa Indica Group]







EEE61915
hypothetical protein OsJ_16648 [Oryza
0.86
2984






sativa Japonica Group]







BAJ96591
predicted protein [Hordeum vulgare subsp.
0.86
2985
3234





vulgare]







CAJ86266
H0901F07.3 [Oryza sativa Indica Group]
0.76
2986
3235



614-632
NP_001183362
hypothetical protein LOC100501771 [Zea
1.00
2987
3236





mays] > gi|238011008|gb|ACR36539.1|








unknown [Zea mays]







XP_002453714
hypothetical protein
0.92
2988
3237





SORBIDRAFT_04g011130 [Sorghum








bicolor] > gi|241933545|gb|EES06690.1|








hypothetical protein








SORBIDRAFT_04g011130 [Sorghum








bicolor]







BAJ91736
predicted protein [Hordeum vulgare subsp.
0.80
2989
3238





vulgare]







NP_001043916
Os01g0687800 [Oryza sativa Japonica
0.76
2990
3239





Group] > gi|18844891|dbj|BAB85360.1|








FAD binding domain containing protein-








like [Oryza sativa Japonica Group]








> gi|113533447|dbj|BAF05830.1|








Os01g0687800 [Oryza sativa Japonica








Group]







EAY75418
hypothetical protein OsI_03321 [Oryza
0.75
2991






sativa Indica Group]






361-379
XP_002441149
hypothetical protein
1.00
2992
3240





SORBIDRAFT_09g021260 [Sorghum








bicolor] > gi|241946434|gb|EES19579.1|








hypothetical protein








SORBIDRAFT_09g021260 [Sorghum








bicolor]







NP_001152458
phosphoribosylanthranilate transferase
0.97
2993
3241





[Zea mays]








> gi|195656517|gb|ACG47726.1|








phosphoribosylanthranilate transferase








[Zea mays]







BAK04138
predicted protein [Hordeum vulgare subsp.
0.89
2994
3242





vulgare]







EEC79273
hypothetical protein OsI_20060 [Oryza
0.90
2995






sativa Indica Group]







NP_001055620
Os05g0429700 [Oryza sativa Japonica
0.90
2996
3243





Group] > gi|55733914|gb|AAV59421.1|








putative anthranilate








phosphoribosyltransferase [Oryza sativa








Japonica Group]








> gi|113579171|dbj|BAF17534.1|








Os05g0429700 [Oryza sativa Japonica








Group]








> gi|215737213|dbj|BAG96142.1|








unnamed protein product [Oryza sativa








Japonica Group]








> gi|222631675|gb|EEE63807.1|








hypothetical protein OsJ_18631 [Oryza








sativa Japonica Group]






149-167
XP_002454239
hypothetical protein
1.00
2997
3244





SORBIDRAFT_04g027330 [Sorghum








bicolor] > gi|241934070|gb|EES07215.1|








hypothetical protein








SORBIDRAFT_04g027330 [Sorghum








bicolor]







ACG32582
heat shock 22 kDa protein [Zea mays]
0.88
2998
3245




NP_001105607
low molecular weight heat shock protein
0.88
2999
3246





precursor [Zea mays]








> gi|3015621|gb|AAC12279.1|low








molecular weight heat shock protein








precursor [Zea mays]








> gi|54299342|gb|AAV32521.1|








mitochondrial small heat shock protein 22








[Zea mays]








> gi|195622718|gb|ACG33189.1|heat








shock 22 kDa protein [Zea mays]







ACF84470
unknown [Zea mays]
0.87
3000
3247




NP_01048175
Os02g0758000 [Oryza sativa Japonica
0.80
3001
3248





Group]








> gi|75294195|sp|Q6Z7V2.1|HS24M_ORYSJ








RecName: Full = 24.1 kDa heat shock








protein, mitochondrial; Short = OsHsp24.1;








Flags: Precursor








> gi|46805691|dbj|BAD17092.1|








putative low molecular weight heat shock








protein [Oryza sativa Japonica Group]








> gi|113537706|dbj|BAF10089.1|








Os02g0758000 [Oryza sativa Japonica








Group] > gi|125541201|gb|EAY87596.1|








hypothetical protein OsI_09007 [Oryza








sativa Indica Group]








> gi|125583753|gb|EAZ24684.1|








hypothetical protein OsJ_08454 [Oryza








sativa Japonica Group]








> gi|215693891|dbj|BAG89090.1|








unnamed protein product [Oryza sativa








Japonica Group]








> gi|215704321|dbj|BAG93755.1|








unnamed protein product [Oryza sativa








Japonica Group]








> gi|215740605|dbj|BAG97261.1|








unnamed protein product [Oryza sativa








Japonica Group]








> gi|313575793|gb|ADR66976.1|22 kDa








heat shock protein [Oryza sativa








Japonica Group]








> gi|332691639|gb|AEE90022.1|








mitochondrial small heat shock protein








[Oryza sativa Japonica Group]






270-288
XP_02444207
hypothetical protein
1.00
3002
3249





SORBIDRAFT_07g014990 [Sorghum








bicolor] > gi|241940557|gb|EES13702.1|








hypothetical protein








SORBIDRAFT_07g014990 [Sorghum








bicolor]







NP_001145845
hypothetical protein LOC100279356 [Zea
0.91
3003
3250





mays] > gi|219884681|gb|ACL52715.1|








unknown [Zea mays]







NP_001061645
Os08g0365900 [Oryza sativa Japonica
0.77
3004
3251





Group] > gi|38636850|dbj|BAD03090.1|








putative nucleolar protein [Oryza sativa








Japonica Group]








> gi|113623614|dbj|BAF23559.1|








Os08g0365900 [Oryza sativa Japonica








Group]








> gi|215717005|dbj|BAG95368.1|








unnamed protein product [Oryza sativa








Japonica Group]








> gi|222640447|gb|EEE68579.1|








hypothetical protein OsJ_27075 [Oryza








sativa Japonica Group]







436-454
Redundant master homologue-skipping






40959
NP_001147431
aconitase2 [Zea mays]
1.00
3005
3252





> gi|195611330|gb|ACG27495.1|








aconitate hydratase, cytoplasmic [Zea








mays] > gi|223948253|gb|ACN28210.1|








unknown [Zea mays]







XP_002445174
hypothetical protein
0.78
3006
3253





SORBIDRAFT_07g005390 [Sorghum








bicolor] > gi|241941524|gb|EES14669.1|








hypothetical protein








SORBIDRAFT_07g005390 [Sorghum








bicolor]







Q6YZX6
RecName: Full = Putative aconitate
0.77
3007






hydratase, cytoplasmic; Short = Aconitase;








AltName: Full = Citrate hydro-lyase








> gi|40253814|dbj|BAD05751.1|








putative Aconitate hydratase [Oryza sativa








Japonica Group]







BAJ85661
predicted protein [Hordeum vulgare subsp.
0.76
3008
3254





vulgare]







CBE71057
aconitate hydratase 3 [Citrus clementina]
0.75
3009
3255




ADZ57218
aconitase protein [Litchi chinensis]
0.74
3010
3256




XP_002524184
aconitase, putative [Ricinus communis]
0.74
3011
3257





> gi|223536553|gb|EEF38199.1|








aconitase, putative [Ricinus communis]







XP_002278138
PREDICTED: hypothetical protein [Vitis
0.74
3012
3258





vinifera]








> gi|297737441|emb|CBI26642.3|








unnamed protein product [Vitis vinifera]







P49608
RecName: Full = Aconitate hydratase,
0.75
3013






cytoplasmic; Short = Aconitase; AltName:








Full = Citrate hydro-lyase








> gi|7437043|pir||T10101 aconitate








hydratase (EC 4.2.1.3) - cucurbit








> gi|868003|dbj|BAA06108.1|aconitase








[Cucurbita cv. Kurokawa Amakuri]







CBE71058
aconitate hydratase 2 [Citrus clementina]
0.74
3014
3259



529-547
XP_002453714
hypothetical protein
1.00
3015
3260





SORBIDRAFT_04g011130 [Sorghum








bicolor] > gi|241933545|gb|EES06690.1|








hypothetical protein








SORBIDRAFT_04g011130 [Sorghum








bicolor]







NP_001183362
hypothetical protein LOC100501771 [Zea
0.85
3016
3261





mays] > gi|238011008|gb|ACR36539.1|








unknown [Zea mays]







BAJ91736
predicted protein [Hordeum vulgare subsp.
0.80
3017
3262





vulgare]







NP_001043916
Os01g0687800 [Oryza sativa Japonica
0.76
3018
3263





Group] > gi|18844891|dbj|BAB85360.1|








FAD binding domain containing protein-








like [Oryza sativa Japonica Group]








> gi|113533447|dbj|BAF05830.1|








Os01g0687800 [Oryza sativa Japonica








Group]







EAY75418
hypothetical protein OsI_03321 [Oryza
0.75
3019






sativa Indica Group]





Predicted
254-273
XP_002489117
hypothetical protein
1.00
3020
3264


siRNA


SORBIDRAFT_0057s002150 [Sorghum





58877


bicolor] > gi|241947368|gb|EES20513.1|








hypothetical protein








SORBIDRAFT_0057s002150 [Sorghum








bicolor]







XP_002488963
hypothetical protein
0.94
3021
3265





SORBIDRAFT_1150s002010 [Sorghum








bicolor] > gi|241946997|gb|EES20142.1|








hypothetical protein








SORBIDRAFT_1150s002010 [Sorghum








bicolor]







XP_002464695
hypothetical protein
0.84
3022
3266





SORBIDRAFT_01g023641 [Sorghum








bicolor]








> gi|241918549|gb|EER91693.1|








hypothetical protein








SORBIDRAFT_01g023641 [Sorghum








bicolor]







XP_002450731
hypothetical protein
0.80
3023
3267





SORBIDRAFT_05g016471 [Sorghum








bicolor] > gi|241936574|gb|EES09719.1|








hypothetical protein








SORBIDRAFT_05g016471 [Sorghum








bicolor]







XP_002450732
hypothetical protein
0.79
3024
3268





SORBIDRAFT_05g016475 [Sorghum








bicolor] > gi|241936575|gb|EES09720.1|








hypothetical protein








SORBIDRAFT_05g016475 [Sorghum








bicolor]





Predicted
632-655
XP_002447941
hypothetical protein
1.00
3025
3269


siRNA


SORBIDRAFT_06g018520 [Sorghum





57179


bicolor] > gi|241939124|gb|EES12269.1|








hypothetical protein








SORBIDRAFT_06g018520 [Sorghum








bicolor]







NP_001159183
hypothetical protein LOC100304268 [Zea
0.99
3026
3270





mays] > gi|195627604|gb|ACG35632.1|








ubiquitin carboxyl-terminal hydrolase 4








[Zea mays]








> gi|223942511|gb|ACN25339.1|








unknown [Zea mays]







NP_001146737
hypothetical protein LOC100280339 [Zea
0.99
3027
3271





mays] > gi|219888545|gb|ACL54647.1|








unknown [Zea mays]








> gi|223974199|gb|ACN31287.1|








unknown [Zea mays]







ACG34260
ubiquitin carboxyl-terminal hydrolase 4
0.99
3028
3272





[Zea mays]







NP_001052951
Os04g0452400 [Oryza sativa Japonica
0.97
3029
3273





Group]








> gi|113564522|dbj|BAF14865.1|








Os04g0452400 [Oryza sativa Japonica








Group]








> gi|215695550|dbj|BAG90741.1|








unnamed protein product [Oryza sativa








Japonica Group]







BAK02826
predicted protein [Hordeum vulgare subsp.
0.96
3030
3274





vulgare]







CAD40853
OSJNBa0086B14.26 [Oryza sativa
0.97
3031






Japonica Group]








> gi|116310175|emb|CAH67188.1|








H0815C01.9 [Oryza sativa Indica Group]








> gi|125548525|gb|EAY94347.1|








hypothetical protein OsI_16114 [Oryza








sativa Indica Group]








> gi|125590577|gb|EAZ30927.1|








hypothetical protein OsJ_15007 [Oryza








sativa Japonica Group]







XP_002270407
PREDICTED: hypothetical protein [Vitis
0.87
3032
3275





vinifera]







XP_002531269
Ubiquitin carboxyl-terminal hydrolase,
0.88
3033
3276





putative [Ricinus communis]








> gi|223529154|gb|EEF31133.1|








Ubiquitin carboxyl-terminal hydrolase,








putative [Ricinus communis]







CAN67091
hypothetical protein VITISV_006756
0.86
3034






[Vitis vinifera]





Predicted
490-510
XP_002446760
hypothetical protein
1.00
3035
3277


siRNA


SORBIDRAFT_06g021990 [Sorghum





55393


bicolor] > gi|241937943|gb|EES11088.1|








hypothetical protein








SORBIDRAFT_06g021990 [Sorghum








bicolor]







ACG37655
anther-specific proline-rich protein APG
0.86
3036
3278





[Zea mays]







ACG35356
anther-specific proline-rich protein APG
0.86
3037
3279





[Zea mays]







NP_001132075
hypothetical protein LOC100193489 [Zea
0.86
3038
3280





mays] > gi|194693356|gb|ACF80762.1|








unknown [Zea mays]







NP_001141295
hypothetical protein LOC100273386 [Zea
0.87
3039
3281





mays] > gi|194703842|gb|ACF86005.1|








unknown [Zea mays]







NP_001053264
Os04g0507700 [Oryza sativa Japonica
0.75
3040
3282





Group]








> gi|32489520|emb|CAE04723.1|








OSJNBa0043L24.11 [Oryza sativa








Japonica Group]








> gi|38567850|emb|CAE05693.2|








OSJNBb0002J11.20 [Oryza sativa








Japonica Group]








> gi|113564835|dbj|BAF15178.1|








Os04g0507700 [Oryza sativa Japonica








Group]








> gi|116310323|emb|CAH67339.1|








OSIGBa0157A06.8 [Oryza sativa Indica








Group]








> gi|116310765|emb|CAH67558.1|








OSIGBa0101P20.1 [Oryza sativa Indica








Group] > gi|125548968|gb|EAY94790.1|








hypothetical protein OsI_16569 [Oryza








sativa Indica Group]








> gi|125590941|gb|EAZ31291.1|








hypothetical protein OsJ_15397 [Oryza








sativa Japonica Group]







BAJ85303
predicted protein [Hordeum vulgare subsp.
0.73
3041
3283





vulgare]






66-86
NP_001104926
MADS2 [Zea mays]
1.00
3042
3284





> gi|29611976|gb|AAO85643.1|MADS-








box transcription factor MADS2 [Zea








mays]







AAG09919
MADS box protein 2 [Zea mays]
1.00
3043
3285




NP_001047230
Os02g0579600 [Oryza sativa Japonica
0.86
3044
3286





Group]








> gi|91207149|sp|Q6EP49.2|MAD27_ORYSJ








RecName: Full = MADS-box








transcription factor 27; AltName:








Full = OsMADS27; AltName:








Full = RMADS218








> gi|30313677|gb|AAO47706.1|








transcription factor MADS27 [Oryza sativa








Japonica Group]








> gi|113536761|dbj|BAF09144.1|








Os02g0579600 [Oryza sativa Japonica








Group]







EEC73475
hypothetical protein OsI_07801 [Oryza
0.84
3045






sativa Indica Group]








> gi|222623119|gb|EEE57251.1|








hypothetical protein OsJ_07263 [Oryza








sativa Japonica Group]







BAK01931
predicted protein [Hordeum vulgare subsp.
0.79
3046
3287





vulgare]







CAM59078
MIKC-type MADS-box transcription
0.79
3047
3288





factor WM30 [Triticum aestivum]







CAD40988
OSJNBa0072F16.13 [Oryza sativa
0.77
3048






Japonica Group]







BAD29571
putative transcription factor MADS27
0.76
3049






[Oryza sativa Japonica Group]






571-591
NP_001047230
Os02g0579600 [Oryza sativa Japonica
1.00
3050
3289





Group]








> gi|91207149|sp|Q6EP49.2|MAD27_ORYSJ








RecName: Full = MADS-box








transcription factor 27; AltName:








Full = OsMADS27; AltName:








Full = RMADS218








> gi|30313677|gb|AAO47706.1|








transcription factor MADS27 [Oryza sativa








Japonica Group]








> gi|113536761|dbj|BAF09144.1|








Os02g0579600 [Oryza sativa Japonica








Group]







EEC73475
hypothetical protein OsI_07801 [Oryza
0.98
3051






sativa Indica Group]








> gi|222623119|gb|EEE57251.1|








hypothetical protein OsJ_07263 [Oryza








sativa Japonica Group]







BAD29571
putative transcription factor MADS27
0.90
3052






[Oryza sativa Japonica Group]







AAG09919
MADS box protein 2 [Zea mays]
0.86
3053
3290




NP_001104926
MADS2 [Zea mays]
0.86
3054
3291





> gi|29611976|gb|AAO85643.1|MADS-








box transcription factor MADS2 [Zea








mays]







BAK01931
predicted protein [Hordeum vulgare subsp.
0.82
3055
3292





vulgare]







CAD40988
OSJNBa0072F16.13 [Oryza sativa
0.80
3056






Japonica Group]







CAM59078
MIKC-type MADS-box transcription
0.81
3057
3293





factor WM30 [Triticum aestivum]






481-501
NP_001132075
hypothetical protein LOC100193489 [Zea
1.00
3058
3294





mays] > gi|194693356|gb|ACF80762.1|








unknown [Zea mays]







ACG35356
anther-specific proline-rich protein APG
1.00
3059
3295





[Zea mays]







ACG37655
anther-specific proline-rich protein APG
0.92
3060
3296





[Zea mays]







NP_001141295
hypothetical protein LOC100273386 [Zea
0.92
3061
3297





mays] > gi|194703842|gb|ACF86005.1|








unknown [Zea mays]







XP_002446760
hypothetical protein
0.91
3062
3298





SORBIDRAFT_06g021990 [Sorghum








bicolor] > gi|241937943|gb|EES11088.1|








hypothetical protein








SORBIDRAFT_06g021990 [Sorghum








bicolor]







NP_001053264
Os04g0507700 [Oryza sativa Japonica
0.81
3063
3299





Group]








> gi|32489520|emb|CAE04723.1|








OSJNBa0043L24.11 [Oryza sativa








Japonica Group]








> gi|38567850|emb|CAE05693.2|








OSJNBb0002J11.20 [Oryza sativa








Japonica Group]








> gi|113564835|dbj|BAF15178.1|








Os04g0507700 [Oryza sativa Japonica








Group]








> gi|116310323|emb|CAH67339.1|








OSIGBa0157A06.8 [Oryza sativa Indica








Group]








> gi|116310765|emb|CAH67558.1|








OSIGBa0101P20.1 [Oryza sativa Indica








Group] > gi|125548968|gb|EAY94790.1|








hypothetical protein OsI_16569 [Oryza








sativa Indica Group]








> gi|125590941|gb|EAZ31291.1|








hypothetical protein OsJ_15397 [Oryza








sativa Japonica Group]







BAJ85303
predicted protein [Hordeum vulgare subsp.
0.78
3064
3300





vulgare]





Predicted
55-75
NP_001167787
hypothetical protein LOC100381480 [Zea
1.00
3065
3301


siRNA


mays] > gi|223943955|gb|ACN26061.1|





59235


unknown [Zea mays]







NP_001143285
hypothetical protein LOC100275819 [Zea
0.97
3066
3302





mays] > gi|195617040|gb|ACG30350.1|








hypothetical protein [Zea mays]





Predicted
512-531
NP_001149853
LOC100283481 [Zea mays]
1.00
3067
3303


siRNA


> gi|195635075|gb|ACG37006.1|pollen-





60123


specific protein like [Zea mays]







NP_001152271
pollen-specific protein like [Zea mays]
0.83
3068
3304





> gi|195638358|gb|ACG38647.1|pollen-








specific protein like [Zea mays]








> gi|195642016|gb|ACG40476.1|pollen-








specific protein like [Zea mays]








> gi|195654521|gb|ACG46728.1|pollen-








specific protein like [Zea mays]







XP_002457577
hypothetical protein
0.71
3069
3305





SORBIDRAFT_03g009730 [Sorghum








bicolor] > gi|241929552|gb|EES02697.1|








hypothetical protein








SORBIDRAFT_03g009730 [Sorghum








bicolor]





Predicted
1216-1237
XP_002455312
hypothetical protein
1.00
3070
3306


siRNA


SORBIDRAFT_03g008220 [Sorghum





56837


bicolor] > gi|241927287|gb|EES00432.1|








hypothetical protein








SORBIDRAFT_03g008220 [Sorghum








bicolor]







NP_001145952
hypothetical protein LOC100279478 [Zea
0.88
3071
3307





mays] > gi|219885097|gb|ACL52923.1|








unknown [Zea mays]







NP_001143749
hypothetical protein LOC100276506 [Zea
0.89
3072
3308





mays] > gi|195626214|gb|ACG34937.1|








hypothetical protein [Zea mays]








> gi|195645208|gb|ACG42072.1|








hypothetical protein [Zea mays]







NP_001131959
hypothetical protein LOC100193355 [Zea
0.84
3073
3309





mays] > gi|194693040|gb|ACF80604.1|








unknown [Zea mays]







NP_001041811
Os01g0112100 [Oryza sativa Japonica
0.72
3074
3310





Group]








> gi|113531342|dbj|BAF03725.1|








Os01g0112100 [Oryza sativa Japonica








Group] > gi|125568749|gb|EAZ10264.1|








hypothetical protein OsJ_00099 [Oryza








sativa Japonica Group]








> gi|215697848|dbj|BAG92041.1|








unnamed protein product [Oryza sativa








Japonica Group]








> gi|215704817|dbj|BAG94845.1|








unnamed protein product [Oryza sativa








Japonica Group]







EAY72253
hypothetical protein OsI_00107 [Oryza
0.72
3075






sativa Indica Group]





Predicted
231-252
NP_001149348
serine carboxypeptidase 1 [Zea mays]
1.00
3076
3311


siRNA


> gi|195626594|gb|ACG35127.1|serine





55404


carboxypeptidase 1 precursor [Zea mays]







NP_001137115
hypothetical protein LOC100217293 [Zea
0.98
3077
3312





mays] > gi|194698414|gb|ACF83291.1|








unknown [Zea mays]







XP_002459781
hypothetical protein
0.72
3078
3313





SORBIDRAFT_02g010510 [Sorghum








bicolor]








> gi|241923158|gb|EER96302.1|








hypothetical protein








SORBIDRAFT_02g010510 [Sorghum








bicolor]






1046-1067
NP_001137115
hypothetical protein LOC100217293 [Zea
1.00
3079
3314





mays] > gi|194698414|gb|ACF83291.1|








unknown [Zea mays]







NP_001149348
serine carboxypeptidase 1 [Zea mays]
0.99
3080
3315





> gi|195626594|gb|ACG35127.1|serine








carboxypeptidase 1 precursor [Zea mays]







XP_002459781
hypothetical protein
0.72
3081
3316





SORBIDRAFT_02g010510 [Sorghum








bicolor]








> gi|241923158|gb|EER96302.1|








hypothetical protein








SORBIDRAFT_02g010510 [Sorghum








bicolor]





Table 8:


Provided are the target Genes of siRNAs Associated with Increased NUE (Table 4) along with their GenBank Accession numbers and sequence identifiers (SEQ ID NO:).


“bind” = binding;


“pos” = position;


“hom” = homologue;


“p.p.” = polypeptide;


“p.n.” = polynucleotide.













TABLE 9







Target genes of mir395, 397 and 398 to be downregulated















Hom.


p.p.
p.n.



Mir
NCBI


SEQ
SEQ


Mir
Bind.
Access


ID
ID


name
Pos.
ion
Annotation/Organism
Identity
NO:
NO:
















zma-miR3
322-342
YP_003208225
ribosomal protein S3 [Coix lacryma-jobi]
1.00
982
1647


98b*


& gt;gi|209361951|gb|ACI43310.1|








ribosomal protein S3 [Coix lacryma-jobi]







NP_043062
ribosomal protein S3 [Zea mays] 
0.99
983
1648





& gt;gi|48478711|ref|YP_024318.1| 








ribosomal protein S3 [Saccharum hybrid cultivar SP-80-3280] 








& gt;gi|50812566 |ref|YP_054668.1|








ribosomal protein S3 [Saccharum officinarum]








& gt;gi|118614530|ref|YP_899446.1 








ribosomal protein S3 [Sorghum bicolor] 








& gt;gi|33933|sp|P06586.1|RR3_MAIZE








RecName: Full = 30S ribosomal protein








S3, chloroplastic & gt;gi|68052946|sp|Q6ENS5.1|RR3_SACOF








RecName: Full = 30S ribosomal protein S3, chloroplastic








& gt;gi|75126330|sp|Q6L3G0.1|RR3_SACHY 








RecName: Full = 30S ribosomal protein S3,








chloroplastic & gt;gi|125987748|sp|A1E9W3.1|RR3_SORBI 








RecName: Full = 30S ribosomal protein S3, 








chloroplastic & gt;gi|12469|emb|CAA68427.1|ribosomal protein S3 








[Zea mays] & gt;gi|902260|emb|CAA60324.1|ribosomal 








protein S3 [Zea mays] & gt;gi|48478613& gt;gi|AAT44633.1








ribosomal protein S3 [Saccharum hybrid cultivar








SP80-3280] & gt;gi|49659550|dbj|BAD27331.1 








ribosomal protein S3 [Saccharum hybrid








& gt;gi|11820164|gb|ABK79534.1








ribosomal protein S3 [Sorghum bicolor]







ADN86110
ribosomal protein S3 [Chasmanthium latifolium]
0.96
984
1649




ADN32929
ribosomal protein S3 [Phyllostachys nigra var. henonis] 
0.96
985






& gt;gi|309321655|gb|ADO65180.1








ribosomal protein S3 [Acidosasa purpurea] 








& gt;gi|309321739|gb|ADO65263.1








ribosomal protein S3 [Ferrocalamus rimosivaginus] 








& gt;gi|309321823|gb|ADO65346.1








ribosomal protein S3 [Indocalamus longiauritus] 








& gt;gi|309321906|gb|ADO65428.14








ribosomal protein S3 [Phllostachys edulis]







YP_003097613
ribosomal protein S3 [Dendrocalamus latiflorus] 
0.96
986
1650





& gt;gi|255040297|gb|ACT99957.1








ribosomal protein S3 [Dendrocalamus latisflorus]







ADD63064
ribosomal protein S3 [Potamophila parviflora]
0.96
987
1651




ADD62997
ribosomal protein S3 [Ortza australiensis]
0.96
988
1652




NP_039424
ribosomal protein S3 [Ortza sativa Japonica Group] 
0.96
989
1653





& gt;gi|50234010|ref|YP_052788.1








ribosomal protein S3 [Ortza nivara]








& gt;gi|09156623|ref|YP_654242.1








ribosomal protein S3 [Oryza sativa Indica Group] 








& gt;gi|68052945|sp|Q6END5.1|RR3_ORYNI








RecName: Full = 30S ribosomal protein S3, chloroplastic 








& gt;gi|48840859|sp|P0C483.1|








RR3_ORYSA RecName: Full = 30S 








ribosomal protein S3, chloroplastic & gt;gi|








148840860|sp|P0C484.1|RR3_ORYSI RecName: 








Full = 30S ribosomal protein S3,








chloroplastic & gt;gi|148840861|sp|P0C485.1|RR3_ORYSA 








RecName: Full = 30S ribosomal protein S3, 








chloroplastic & gt;gi|l2025|emb|CAA33934.1








ribosomal protein S3 [Ortza sativa Japonica Group] 








& gt;gi|42795515|gb|AAS46082.1








ribosomal protein S3 [Ortza sativa Indica Group] 








& gt;gi|42795581|gb|AAS46147.1








ribosomal protein S3 [Ortza sativa Japonica Group] 








& gt;gi|4279564|gb|AAS46210.1








ribosomal protein S3 [Ortza sativa Japonica Group] 








& gt;gi|49615034|dbj|BAD26817.1








ribosomal protein S3 [Ortza nivara] & gt;gi|50725165|dbj|BAD33782.1








ribosomal protein S3 [Ortza sativa Japonica Group] 








& gt;gi|l51091521|dbj|BAD36259.1








ribosomal protein S3 [Ortza sativa Japonica Group] 








& gt;gi|8200203|gb|EEC82630.1








hypothetical protein OsI_27221 [Oryza sativa Indica Group] 








& gt;gi|218202023|gb|EEC84450.1








hypothetical protein OsI_31072








[Oryza sativa Indica Group] & gt;gi|290790601|gb|ADD62861.1








ribosomal protein S3 [Ortza sativa Japonica Group] 








& gt;gi|290790670|gb|ADD62929.1








ribosomal protein S3 [Oryza meridionalis] & gt;gi|226646|prf|








1603356BW ribosomal protein S3







ADO65512
ribosomal protein S3 [Bambusa emeiensis]
0.95
990





BAD05516
ribosomal protein S3 [Ortza sativa Japonica Group]
0.95
991
1654


osa-
426-446
XP_002463896
hypothetical protein SORBIDRAFT_01g008450 
1.00
1249
1889


miR395m


[Sorghum bicolor] & gt;gi|241917750|gb|EER90894.1








SORBIDRAFT_01g008450 [Sorghum bicolor]







ACN28609
unknown [Zea mays]
0.95
1250
1890




ACN34023
unknown [Zea mays]
0.95
1251
1891




ACG45192
bifunctional 3-phosphoadenosine 5-
0.94
1252
1892





phosphosulfate synthtase [Zea mays]







NP_001104877
ATP sulfurylase [Zea mays] & gt;gi|2738750|gb|AAB94542.1
0.95
1253
1893





ATP sulfurylase [Zea mays]







EAY91825
hypothetical protein OsI_13470 [Oryza sativa Indica Group]
0.80
1254





NP_001051234
Os03g0743900 [Oryza sativa Japonica Group] 
0.80
1255
1894





& gt;gi|30017582|gb|AAP13004.1








putative ATP sulfurylase [Oryza sativa Japonica Group] 








& gt;gi|108711024|gb|ABF98819.1








Bifunctional 3 & apos;-phosphoadenosine 5 & apos;-phosphosulfate 








synthethase, putative, expressed [Oryza sativa Japonica Group] 








& gt;gi|113549705|dbj|BAF13148.1 Os03g0743900








[Oryza sativa Japonica Group] & gt;gi|215704581|dbj|BAG94214.1








unnamed protein product [Oryza sativa Japonica Group] 







BAK05662
predicted protein [Hordeum vulgare subsp. vulgare] 
0.79
1256
1895





& gt;gi|326502564|dbj|BAJ95345.1








predicted protein [Hordeum vulgare subsp. vulgare]







BAA36274
plastidic ATP sulfurylase [Oryza sativa Indica Group]
0.80
1257
1896




EAZ28548
hypothetical protein OsJ_12530 [Oryza sativa Japonica Group]
0.77
1258




352-372
XP_002441904
hypothetical protein SORBIDRAFT_08g004650 
1.00
1259
1897





[Sorghum bicolor] & gt;gi|241942597|gb|EES15742.1








hypothetical protein SORBIDRAFT_08g004650 [Sorghum bicolor]







EEE52851
hypothetical protein OsJ_35390 [Oryza sativa Japonica Group]
0.71
1260





NP_001066285
Os12g0174100 [Oryza sativa Japonica Group] 
0.71
1261
1898





& gt;gi|77553790|gb|ABA96586.1








Growth regulator protein, putative, expressed 








[Oryza sativa Japonica Group] & gt;gi|








255670095|dbj|BAF29304.2 Os12g0174100








[Oryza sativa Japonica Group]







EEC68940
hypothetical protein OsI_37646 [Oryza sativa Indica Group]
0.70
1262




528-548
ACN34023
unknown [Zea mays]
1.00
1263
1899




NP_001104877
ATP sulfurylase [Zea mays] & gt;gi|2738750|gb|AAB94542.1
0.98
1264
1900





ATP sulfurylase [Zea mays]







ACN28609
unknown [Zea mays]
0.94
1265
1901




ACG41592
bifunctional 3-phosphoadenosine 5-
0.94
1266
1902





phosphosulfate synthetase [Zea mays]







XP_002463896
hypothetical protein SORBIDRAFT_01g008450
0.94
1267
1903





[Sorghum bicolor] & gt;gi|241917750|gb|EER90894.1








hypothetical protein SORBIDRAFT_01g008450 [Sorghum bicolor]







BAK05662
predicted protein [Hordeum vulgare subsp. vulgare] 
0.84
1268
1904





& gt;gi|326502564|dbj|BAJ95345.1








predicted protein [Hordeum vulgare subsp. vulfare]







EAY91825
hypothetical protein OsI_13470 [Oryza sativa Indica Group]
0.80
1269





NP_001051234
Os03g0743900 [Oryza sativa Japonica Group] 
0.79
1270
1905





& gt;gi|30017582|gb|AAP13004.1








putative ATP sulfurylase [Oryza sativa Japonica Group] 








& gt;gi|108711024|gb|ABF98819.1








Bifunctional 3 & apos;-phosphoadenosine 5 &








aps;-phosphosulfate synthethase, putative, expressed








[Oryza sativa Japonica Group] & gt;gi|13549705|dbj|








BAF13148.1Os03g0743900








[Oryza sativa Japonica Group] & gt;gi|215704581|dbj|BAG94214.1 








unnamed protein product 








[Oryza sativa Japonica Group] 







BAA36274
plastidic ATP sulfurylase [Oryza sativa Indica Group]
0.79
1271
1906




EAZ28548
hypothetical protein OsJ_12530 [Oryza sativa Japonica Group]
0.76
1272




305-325
ACL53345
unknown [Zea mays]
1.00
1273
1907




XP_002465703
hypothetical protein SORBIDRAFT_01g044100 [Sorghum bicolor] 
0.95
1274
1908





& gt;gi|241919557|gb|EER92701.1








hypothetical protein SORBIDRAFT_01g044100 [Sorghum bicolor]







BAJ85215
predicted protein [Hordeum vulgare subsp. vulgare] 
0.85
1275
1909





& gt;gi|326511599|dbj|BAJ91944.1








predicted protein [Hordeum vulgare subsp. vulfare] 








& gt;gi|25585257|gb|EAZ25921.1








hypothetical protein OsJ_09764 [Oryza sativa Japonica Group]







AAN59769
Putative sulfate transporter [Oryza sativa Japonica Group]
0.85
1276






& gt;gi|125585257|gb|EAZ25921.1|








hypothetical protein OsJ_09764 [Oryza sativa Japonica Group]







EEC74682
hypothetical protein OsI_10373 [Oryza sativa Japonica Group]
0.85
1277





EEC74681
hypothetical protein OsI_10372 [Oryza sativa Japonica Group]
0.72
1278





EEE58498
hypothetical protein OsJ_09763 [Oryza sativa Japonica Group]
0.72
1279





NP_001049257
Os03g0195300 [Oryza sativa Japonica Group] 
0.72
1280
1910





& gt;gi|108706649|gb|ABF94444.1








Sulfate transporter 2.1, putative, 








expressed [Oryza sativa Japonica Group]








& gt;gi|13547728|dbj|BAF11171.1 








Os03g0195300 [Oryza sativa Japonica Group]





zma-
798-818
XP_002458747
hypothetical protein SORBIDRAFT_03g039530 [Sorghum bicolor] 
1.00
1321
1949


miR397a


& gt;gi|241930722|gb|EES03867.1








hypothetical protein SORBIDRAFT_03g039530 [Sorghum bicolor]







NP_001147942
L-ascorbate oxidase [Zea mays] & gt;gi|195614732|gb|ACG29196.1
0.96
1322
1950





L-ascorbate oxidase precursor [Zea mays]







ACN34362
unknown [Zea mays]
0.96
1323
1951




EEC71780
hypothetical protein OsI_04394 [Oryza sativa Indica Group]
0.86
1324





NP_001044773
Os03g0842500 [Oryza sativa Japonica Group] 
0.86
1325
1952





& gt;gi|19571025|gb|BAB86452.1








putative laccase LAC5-6 [Oryza sativa Japonica Group] 








& gt;gi|13534304|dbj|BAF06687.1








Os01g0842500 [Oryza sativa Japonica Group] 








& gt;gi|125572601|gb|EAZ14116.1








hypothetical protein OsJ_04040 [Oryza sativa Japonica Group] 








& gt;gi|21569481|dbj|NAG90005.1








unnamed protein product [Oryza sativa Japonica Group]







BAJ84890
predicted protein [Hordeum vulgare subsp. vulgare]
0.84
1326
1953




BAJ96691
predicted protein [Hordeum vulgare subsp. vulgare]
0.84
1327
1954




NP_001105875
putative laccase [Zea mays] 
0.79
1328
1955





& gt;gi|8461878|emb|CAJ30500.1 putative laccase [Zea mays]







ACN28855
unknown [Zea mays]
0.79
1329
1956




XP_002458746
hypothetical protein SORBIDRAFT_03g039520 
0.78
1330
1957





[Sorghum bicolor] & gt;gi|241930721|gb|EES03866.1








hypothetical protein SORBIDRAFT_03g039520 [Sorghum bicolor]






458-478
ACF87912
unknown [Zea mays]
1.00
1331
1958




NP_001105951
LOC100037774 [Zea mays] & gt;gi|1184776|gb|AAA87580.1 
0.99
1332
1959





cytosolic glyceraldehyde-3-phosphate 








dehydrogenase GAPC4 [Zea mays]







NP_001105385
glyceraldehyde-3-phosphate dehydrogenase, 
0.99
1333
1960





cytosolic 3 [Zea mays]








& gt;gi|6166167|sp|Q43247.1G3PE_MAIZE RecName: 








Full = Glyceraldehyde-3-phosphate








dehydrogense, cytosolic 3 & gt;gi|184774|gb|AAA87579.1 








cytosolic glyceraldehyde-3-phosphate 








dehydrogenase GAPC3 [Zea mays]







ACG36109
glyceraldehyde-3-phosphate dehydrogenase, 
0.99
1334
1961





cytosolic 3 [Zea mays]







ADZ55283
glyceraldehyde-3-phosphate dehydrogenase [Zea mays]
0.98
1335
1962




XP_002452401
hypothetical protein SORBIDRAFT_04g025120
0.98
1336
1963





[Sorghum bicolor] & gt;gi|241932232|gb|EES05377.1








hypothetical protein SORBIDRAFT_04g025120 [Sorghum bicolor]







NP_001053139
Os04g0486600 [Oryza sativa Japonica Group] 
0.92
1337
1964





& gt;gi|3834606|emb|CAE02009.2








OJ0002233_09.15 [Oryza sativa Japonica Group] 








& gt;gi|90265255|emb|CAD79700.2H0302E05.3








[Oryza sativa Japonica Group] & gt;gi|13564710|dbj|BAF15053.1 








Os05g0486600 [Oryza sativa Japonica Group] 








& gt;gi|69244439|gb|ACA50493.1 glyceraldehyde- 








3-phosphate dehydrogenase








[Oryza sativa Japonica Group] & gt;gi|215694303|dbj|BAG89296.1








unnamed protein product [Oryza sativa Japonica Group]








& gt;gi|306415975|gb|ADM86862.1 glyceraldehyde-3-phosphate








dehydrogenase [Oryza sativa Japonica Group] 







NP_001047348
Os02g0601300 [Oryza sativa Japonica Group] 
0.92
1338
1965





& gt;gi|47848293|dbj|BAD22157.1 putative








glyceraldehyde-3-phosphate dehydrogenase (phosporylating) 








[Oryza sativa Japonica Group] 








& gt;gi|13536879|dbj|BAF09262.1 Os02g0601300 








[Oryza sativa Japonica Group] 








& gt;gi|119434402|gb|ABL75274.1 glyceralde-3-








phosphate dehydrogenase [Oryza sativa] 








& gt;gi|215704481|dbjBAG93915.1| unnamed protein product








[Oryza sativa Japonica Group]








& gt;gi|306415941|gb|ADM86845.1 glyceraldehyde- 








3-phosphate dehydrogenase








[Oryza sativa Japonica Group]







EEC73541
hypothetical protein OsI_07948 [Oryza sativa Indica Group]
0.91
1339





ABQ81648
glyceraldehyde-3-phosphate dehydrogenase [Triticum aestivum]
0.91
1340
1966



201-221
XP_002458746
hypothetical protein SORBIDRAFT_03g039520
1.00
1341
1967





[Sorghum bicolor] & gt;gi|241930721|gb|EES03866.1








hypothetical protein SORBIDRAFT_03g039520 [Sorghum bicolor]







NP_001105875
putative laccase [Zea mays] & gt;gi|84618781|emb|CAJ30500.1 
0.94
1342
1968





putative laccase [Zea mays]







NP_001146658
hypothetical protein LOC100280258 
0.93
1343
1969





[Zea mays] & gt;gi|219888209|gb|ACL54479.1 








unknown [Zea mays]







ACN28855
unknown [Zea mays]
0.94
1344
1970




NP_001044772
Os01g0842400 [Ortza satuva Japonica Group] 
0.80
1345
1971





& gt;gi|75321217|sp|Q5N9X2.1|LAC4_ORYSJ 








RecName: Full = Laccase-4; AltName:








Full = Benzenediol: oxygen oxidoreductase 4; AltName:








Full = Diphenol oxidase 4; AltName: 








Full = Urishiol oxidase 4; flags: precursor








& gt;gi|56784239|dbj|BAD81734.1| putative laccase








LAC5-6 [Oryza sativa Japonica Group] 








& gt;gi|13534303|dbj|BAF06686.1|Os01g0842400 








[Oryza sativa Japonica Group] & gt;gi|l215697155








|dbj|BAG91149.1| unnamed protein product








[Oryza sativa Japonica Group]







EAZ14115
hypothetical protein OsJ_04039 [Oryza sativa Japonica Group]
0.80
1346





EEC71777
hypothetical protein OsJ_04389 [Oryza sativa Indica Group]
0.80
1347





BAJ99773
predicted protein [Hordeum vulgare subsp. vulgare)
0.78
1348
1972




XP_002458747
hypothetical protein SORBIDRAFT_03g039530 
0.78
1349
1973





[Sorghum bicolor] & gt;gi|241930722|gb|EES03867.1








hypothetical protein SORBIDRAFT_03g039530 [Sorghum bicolor]







AAC04576
putative high-pI laccase [Oryza sativa Japonica Group]
0.79
1350
1974


mtr-
426-446
XP_002463896
hypothetical protein SORBIDRAFT_01g008450 
1.00
1351
1975


miR395c


[Sorghum bicolor] & gt;gi|241917750|gb|EER90894.1








hypothetical protein SORBIDRAFT_01g008450 [Sorghum bicolor]







ACN28609
unknown [Zea mays]
0.95
1352
1976




ACN34023
unknown [Zea mays]
0.95
1353
1977




ACG45192
bifunctional 3-phosphoadenosine 5-phosphosulfate 
0.94
1354
1978





synthtase [Zea mays]







NP_001104877
ATP sulfurylase [Zea mays] & gt;gi|2738750|gb|AAB94542.1
0.95
1355
1979





ATP sulfurylase [Zea mays]







EAY91825
hypothetical protein OsI_13470 [Oryza sativa Indica Group]
0.80
1356





NP_001051234
Os03g0743900 [Oryza sativa Japonica Group] 
0.80
1357
1980





& gt;gi|30017582|gb|AAP13004.1 putative








ATP sulfurylase [Oryza sativa Japonica Group] 








& gt;gi|108711024|gb|ABF98819.1








Bifunctional 3 & apos;-phosphoadenenosine 5 & apos;-








phosphosulfate synthethase, putative, expressed








[Oryza sativa Japonica Group] 








& gt;gi|13549705|dbj|BAF13148.1 Os03g0743900








[Oryza sativa Japonica Group] & gt;gi|215704581|dbj|








BAG94214.1 unnamed protein product








[Oryza sativa Japonica Group] 







BAK05662
predicted protein [Hordeum vulfare subsp. vulgare] 
0.79
1358
1981





& gt;gi|326502564|dbj|BAJ95345.1 predicted








protein product [Hordeum vulgare subsp. vulgare]







BAA36274
plastidic ATP sulfurylase [Oryza sativa Indica Group]
0.80
1359
1982




EAZ28548
hypothetical protein OsJ_12530 [Oryza sativa Japonica Group]
0.77
1360




528-548
ACN34023
unknown [Zea mays]
1.00
1361
1983




NP_001104877
ATP sulfurylase [Zea mays] & gt;gi|2738750|gb|AAB94542.1
0.98
1362
1984





ATP sulfurylase [Zea mays]







ACN28609
unknown [Zea mays]
0.94
1363
1985




ACG45192
bifunctional 3-phosphoadenosine 
0.94
1364
1986





5-phosphosulfate synthetase [Zea mays]







XP_002463896
hypothetical protein SORBIDRAFT_01g008450 
0.94
1365
1987





[Sorghum bicolor] & gt;gi|241917750|gb|EER90894.1








hypothetical protein SORBIDRAFT_01g008450 [Sorghum bicolor]







BAK05662
predicted protein [Hordeum vulgare subsp. vulgare] 
0.84
1366
1988





& gt;gi|326502564|dbj|BAJ95345.1 predicted








protein [Hordeum vulgare subsp. vulgare]







EAY91825
hypothetical protein OsI_13470 [Oryza sativa Indica Group]
0.80
1367





NP_001051234
Os03g0743900 [Oryza sativa Japonica Group] 
0.79
1368
1989





& gt;gi|30017582|gb|AAP13004.1 








putative ATP sulfurylase [Oryza sativa Japonica Group] 








& gt;gi|10871102|gb|ABF98819.1 Bifunctional 3 & apos;-








phosphoadenosine 5 & apos;-phosphosulfate synthethase, putative, 








expressed [Oryza sativa Japonica Group] 








& gt;gi|113549705|dbj|BAF13148.1Os03g0743900








[Oryza sativa Japonica Group] 








& gt;gi|215704581|dbj|BAG94214.1 unnamed protein product








[Oryza sativa Japonica Group]







BAA36274
plastidic ATP sulfurylase [Oryza sativa Indica Group]
0.79
1369
1990




EAZ28548
hypothetical protein OsJ_12530 [Oryza sativa Japonica Group]
0.76
1370




359-379
XP_002452295
hypothetical protein SORBIDRAFT_04g023180 
1.00
1371
1991





[Sorghum bicolor] & gt;gi|241932126|gb|EES05271.1








hypothetical protein SORBIDRAFT_04g023180








[Sorghum bicolor] 







NP_001148591
2-hydrozy-3-oxopropionate reductase 
0.98
1372
1992





[Zea mays] & gt;gi|95620656|gb|ACG32158.1








2-hydrozy-3-oxopropionate reductase [Zea mays]







NP_001047154
Os02g0562700 [Oryza sativa Japonica Group] 
0.92
1373
1993





& gt;gi|29368238|gb|AAAO72678.1 gamma








hydroxybutyrate dehydrogenase-like protein 








[Oryza sativa Japonica Group]








& gt;gi|32352132|dbj|BAC78559.1 








hypothetical protein [Oryza sativa Japonica Group]








& gt;gi|46390142|dbj|BAD15576.1 








putative gamma hydroxybutyrate dehydrogenase 








[Oryza sativa Japonica Group] & gt;gi|13536685|dbj|








BAF09068.1|Os02g562700








[Oryza sativa Japonica Group] 








& gt;gi|215679041|dbj|BAG96471.1 unnamed protein product








[Oryza sativa Japonica Group] 








& gt;gi|21501279|dbj|BAG92703.1 unnamed protein product








[Oryza sativa Japonica Group] 








& gt;gi|215766587|dbj|BAG98746.1 unnamed protein product








[Oryza sativa Japonica Group] 








& gt;gi|l218190991|gb|EEC73418.1 hypothetical protein








OsI_07685 [Oryza sativa Indica Group] 








& gt;gi|222623072|gb|EEE57204.1








hypothetical protein OsJ_07161 [Oryza sativa Japonica Group]







BAJ91168
predicted protein [Hordeum vulgare subsp. vulgare] 
0.90
1374
1994





& gt;gi|326518654|dbj|BAJ88356.1








predicted protein [Hordeum vulgare subsp. vulgare]







XP_002266252
PREDICTED: hypothetical protein isoform 1
0.80
1375
1995





[Vitis vinifera] & gt;gi|47861759|emb|CAN78910.1








hypothetical protein VITISV_032948 [Vitis vinifera]








& gt;gi|297736660|emb|CBI25677.3








unnamed protein product [Vitis vinifera]







ACU2717
unknown [Glycine max]
0.80
1376
1996




XP_002266296
PREDICTED: hypothetical protein isoform 2 [Vitis vinifera]
0.78
1377
1997




XP_002320548
predicted protein [Populus trichocarpa] 
0.76
1378
1998





& gt;gi|222861321|gb|EEE98863.1 predicted protein








[Populus trichocarpa]







BAG16485
succinic semialdehyde reductase isofom1 [Solanum lycopersicum]
0.79
1379
1999




ABK22179
unknown [Picea sitchensis]
0.77
1380
2000



305-325
ACL53345
unknown [Zea mays]
1.00
1381
2001




XP_002465703
hypothetical protein SORBIDRAFT_01g044100 
0.95
1382
2002





[Sorghum bicolor] & gt;gi|241919557|gb|EER92701.1








hypothetical protein SORBIDRAFT_01g044100 [Sorghum bicolor]







BAJ85215
predicted protein [Hordeum vulgare subsp. vulgare] 
0.85
1383
2003





& gt;gi|326511599|dbj|BAJ91944.1








predicted protein [Hordeum vulgare subsp. vulgare]







AAN59769
Putative sulfate transporter [Oryza sativa Japonica Group] 
0.85
1384






& gt;gi|25585257|gb|EAZ25921.1








hypothetical protein OsJ_09764 [Oryza sativa Japonica Group]







EEC74682
hypothetical protein OsJ_10373 [Oryza sativa Indica Group]
0.85
1385





EEC74681
hypothetical protein OsJ_10372 [Oryza sativa Indica Group]
0.72
1386





EEE58498
hypothetical protein OsJ_09763[Oryza sativa Japonica Group]
0.72
1387





NP_001049257
Os03g0195300 [Oryza sativa Japonica Group] 
0.72
1388
2004





& gt;gi|108706649|gb|ABF94444.1








Sulfate transporter 2.1, putative, expressed 








[Oryza sativa Japonica Group]








& gt;gi|13547728|dbj|BAF11171.1Os03g0195300 








[Oryza sativa Japonica Group] 





Predicted 
201-221
XP_002456833
hypothetical protein SORBIDRAFT_03g043750 
1.00
1311
1939


zmamir


[Sorghum bicolor] & gt;gi|241928808|gb|EES01953.1





50266


hypothetical protein SORBIDRAFT_03g043750 








[Sorghum bicolor]







NP_001131338
hypothetical protein LOC100192654 
0.96
1312
1940





[Zea mays] & gt;gi|94690154|gb|ACF79161.1








unknown [Zea mays] & gt;gi|219884631|gb|ACL52690.1








unknown [Zea mays] 








& gt;gil238908563|gb|ACF79698.2unknown [Zea mays]







ACL52654
unknown [Zea mays]
0.92
1313
1941




ACG35805
selenium-binding protein [Zea mays]
0.92
1314
1942




NP_001045194
Os01g0916400 [Oryza sativa Japonica Group] 
0.90
1315
1943





& gt;gi|19386752|dbj|BAB86133.1 








putative selenium binding protein [Oryza sativa Japonica Group] 








& gt;gi|20805006|dbj|BAB92682.1 putative selenium 








binding protein [Oryza sativa Japonica Group] 








& gt;gi|31322241|gb|AAO91777.1 








putative selenium binding protein








[Oryza sativa Japonica Group] & gt;gi|113534725|dbj|








BAF07108.1|Os01g0916400








[Oryza sativa Japonica Group] 








& gt;gi|125528845|gb|EAY76959.1|








hypothetical protein








OsI_04917 [Oryza sativa Indica Group] 








& gt;gi|25573100|gb|EAZ14615.1|








hypothetical protein OsJ_04540 








[Oryza sativa Japonica Group] 








& gt;gi|13534725|dbj|BAF07108.1OS01g0916400








[Oryza sativa Japonica Group] 







BAJ94729
predicted protein [Hordeum vulgare subsp. vulgare]
0.88
1316
1944




BAB40923
putative selenium binding protein 
0.88
1317
1945





[Oryza sativa (japonica cultivar-group)]







XP_002328915
predicted protein [Populus trichocarpa] 
0.80
1318
1946





& gt;gi|222839345|gb|EEE77682.1








predicted protein [Populus trichocarpa]







XP_002298672
predicted protein [Populus trichocarpa] 
0.80
1319
1947





& gt;gi|222845930|gb|EEE3477.1








predicted protein [Populus trichocarpa]







XP_002520613
selenium-binding protein, putative [Ricinus communis] 
0.80
1320
1948





& gt;gi|223540174|gb|EEF41749.1








selenium-binding protein, putative [Ricinus communis]





Table 9, provided are target genes to be downregulated, of mirs 395, 397 and 398.













TABLE 10







Abbreviations of plant species


Provided are the abbreviations and full names of plant species.









Abbreviation
Organism Name
Common Name





ahy
Arachis hypogaea
Peanut


aly
Arabidopsis lyrata
Arabidopsis lyrata


aqc
Aquilegia coerulea
Rocky Mountain Columbine


ata
Aegilops taushii
Tausch's goatgrass


ath
Arabidopsis thaliana
Arabidopsis thaliana


bdi
Brachypodium distachyon
Grass


bna
Brassica napus
Brassica napus canola (“liftit”)


bol
Brassica oleracea
Brassica oleracea wild cabbage


bra
Brassica rapa
Brassica rapa yellow mustard


ccl
Citrus clementine
Clementine


csi
Citrus sinensis
Orange


ctr
Citrus trifoliata
Trifoliate orange


gma
Glycine max
Glycine max


gso
Glycine soja
Wild soybean


hvu
Hordeum vulgare
Barley


lja
Lotus japonicus
Lotus japonicus


mtr
Medicago truncatula
Medicago truncatula—Barrel 




Clover (“tiltan”)


osa
Oryza sativa
Oryza sativa


pab
Picea abies
European spruce


ppt
Physcomitrella patens
Physcomitrella patens (moss)


pta
Pinus taeda
Pinus taeda—Loblolly Pine


ptc
Populus trichocarpa 
Populus trichocarpa—black 




cotton wood


rco
Ricinus communis
Castor bean (“kikayon”)


sbi
Sorghum bicolor
Sorghum bicolor Dura


sly
Solanum lycopersicum
tomato microtom


smo
Selaginella moellendorffii 
Selaginella moellendorffii


sof
Saccharum officinarum
Sugarcane


ssp
Saccharum spp
Sugarcane


tae
Triticum aestivum
Triticum aestivum


tcc
Theobroma cacao
cacao tree and cocoa tree


vvi
Vitis vinifera
Vitis vinifera Grapes


zma
Zea mays
corn









Example 3
Gene Cloning and Creation of Binary Vectors for Plant Expression
The predicted target sequences were cloned into binary vectors for the generation of transgenic plants. The full-length open reading frame (ORF) was synthesized by Genscript (Israel). The resultant clone was digested with appropriate restriction enzymes and inserted into the Multi Cloning Site (MCS) of a similarly digested binary vector through ligation using T4 DNA ligase enzyme (Promega, Madison, Wis., USA). FIG. 1 is a plasmid map of the binary vector pORE-E1, used for plant transformation.
Example 4
Generation of Transgenic Model Plants Expressing the Target Genes/Silencing Agents of Some Embodiments of the Invention
Arabidoposis thaliana transformation was performed using the floral dip procedure following a slightly modified version of the published protocol (Clough and Bent, 1998, Plant J 16(6): 735-43; Desfeux et al, 2000, Plant Physiol. 123(3): 895-904). Briefly, T0 Plants were planted in small pots filled with soil. The pots were covered with aluminum foil and a plastic dome, kept at 4° C. for 3-4 days, then uncovered and incubated in a growth chamber at 24° C. under 16 hr light:8 hr dark cycles. A week prior to transformation all individual flowering stems were removed to allow for growth of multiple flowering stems instead. A single colony of Agrobacterium (GV3101) carrying the binary vectors (pORE-E1), harboring the target gene or silencing sequence was cultured in LB medium supplemented with kanamycin (50 mg/L) and gentamycin (25 mg/L). Three days prior to transformation, each culture was incubated at 28° C. for 48 hrs, shaking at 180 rpm. The starter culture was split the day before transformation into two cultures, which were allowed to grow further at 28° C. for 24 hours at 180 rpm. Pellets containing the agrobacterium cells were obtained by centrifugation of the cultures at 5000 rpm for 15 minutes. The pellets were resuspended in an infiltration medium (10 mM MgCl2, 5% sucrose, 0.044 μM BAP (Sigma) and 0.03% Tween 20) in double-distilled water.
Transformation of T0 plants was performed by inverting each plant into the Agrobacterium suspension, keeping the flowering stem submerged for 5 minutes. Following inoculation, each plant was blotted dry for 5 minutes on both sides, and placed sideways on a fresh covered tray for 24 hours at 22° C. Transformed (transgenic) plants were then uncovered and transferred to a greenhouse for recovery and maturation. The transgenic T0 plants were grown in the greenhouse for 3-5 weeks until the seeds are ready. The seeds were then harvested from plants and kept at room temperature until sowing.
Example 5
Selection of Transgenic Arabidopsis Plants Expressing the Nucleic Acid Molecules of Some Embodiments of the Invention According to Expression Level
Arabidopsis seeds were sown. One to 2 weeks old seedlings were sprayed with a non-volatile herbicide, Basta (Bayer) at least twice every few days. Only resistant plants, which are heterozygous for the transgene, survive. PCR on the genomic gene sequence was performed on the surviving seedlings using primers pORE-F2 (fwd, 5′-TTTAGCGATGAACTTCACTC-3′/SEQ ID NO:310) and a custom designed reverse primer based on each target sequence.
Example 6
Evaluating Changes in Root Architecture in Transgenic Plants
Root architecture of the plant governs multiple key agricultural traits. Root size and depth have been shown to logically correlate with drought tolerance and enhanced NUE, since deeper and more branched root systems provide better soil coverage and can access water and nutrients stored in deeper soil layers.
To test whether the transgenic plants produce a modified root structure, plants were grown in agar plates placed vertically. A digital picture of the plates was taken every few days and the maximal length and total area covered by the plant roots were assessed. From every construct created, several independent transformation events were checked in replicates. To assess significant differences between root features, statistical test, such as a Student's t-test, was employed in order to identify enhanced root features and to provide a statistical value to the findings.
Example 7
Testing for Increased Nitrogen Use Efficiency (NUE)
To analyze whether the transgenic Arabidopsis plants are more responsive to nitrogen, plants were grown in two different nitrogen concentrations: (1) optimal nitrogen concentration (100% NH4NO3, which corresponds to 20.61 mM) or (2) nitrogen deficient conditions (1% or 10% NH4NO3, which corresponds to 0.2 and 2.06 mM, respectively). Plants were allowed to grow until seed production followed by an analysis of their overall size, time to flowering, yield, protein content of shoot and/or grain, and seed production. The parameters checked are each of the overall size of the plant, wet and dry weight, the weight of the seeds yielded, the average seed size and the number of seeds produced per plant. Other parameters that are tested include: the chlorophyll content of leaves (as nitrogen plant status and the degree of leaf greenness are highly correlated), amino acid and the total protein content of the seeds or other plant parts such as leaves or shoots and oil content. Transformed plants not exhibiting substantial physiological and/or morphological effects, or exhibiting higher measured parameters levels than wild-type plants, are identified as nitrogen use efficient plants.
Although the invention has been described in conjunction with specific embodiments thereof, it is evident that many alternatives, modifications and variations will be apparent to those skilled in the art. Accordingly, it is intended to embrace all such alternatives, modifications and variations that fall within the spirit and broad scope of the appended claims.
All publications, patents and patent applications mentioned in this specification are herein incorporated in their entirety by reference into the specification, to the same extent as if each individual publication, patent or patent application was specifically and individually indicated to be incorporated herein by reference. In addition, citation or identification of any reference in this application shall not be construed as an admission that such reference is available as prior art to the present invention. To the extent that section headings are used, they should not be construed as necessarily limiting.

## Claims

1. A method of improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of a plant, the method comprising expressing within the plant an exogenous polynucleotide which downregulates an activity or expression of a polypeptide having an amino acid sequence 100% homologous to SEQ ID NOs: 312 and 317, wherein said polypeptide is capable of regulating nitrogen use efficiency of the plant, thereby improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of the plant.

2. A transgenic plant exogenously expressing a polynucleotide which downregulates an activity or expression of a polypeptide having an amino acid sequence 100% homologous to SEQ ID NOs: 312 and 317, wherein said polypeptide is capable of regulating nitrogen use efficiency of the plant.

3. A nucleic acid construct comprising a polynucleotide which downregulates an activity or expression of a polypeptide having an amino acid sequence 100% homologous to SEQ ID NOs: 312 and 317, wherein said polypeptide is capable of regulating nitrogen use efficiency of a plant, said nucleic acid sequence being under the regulation of a cis-acting regulatory element.

4. The method of claim 1, the transgenic plant of claim 2 or the nucleic acid construct of claim 3, wherein said polynucleotide acts by a mechanism selected from the group consisting of sense suppression, antisense suppression, ribozyme inhibition, gene disruption.

5. The nucleic acid construct of claim 3, wherein said cis-acting regulatory element comprises a promoter.

6. The nucleic acid construct of claim 5, wherein said promoter comprises a tissue-specific promoter.

7. The nucleic acid construct of claim 6, wherein said tissue-specific promoter comprises a root specific promoter.

8. The method of claim 1, further comprising growing the plant under limiting nitrogen conditions.

9. The method of claim 1, further comprising growing the plant under abiotic stress.

10. The method of claim 9, wherein said abiotic stress is selected from the group consisting of salinity, drought, water deprivation, flood, etiolation, low temperature, high temperature, heavy metal toxicity, anaerobiosis, nutrient deficiency, nutrient excess, atmospheric pollution and UV irradiation.

11. The method of claim 1 wherein the plant is a monocotyledon.

12. The method of claim 1, wherein the plant is a dicotyledon.