# Patent

## Title
Cell penetrating peptide, conjugate comprising same, and composition comprising conjugate

## Abstract

The present invention relates to a conjugate of cell penetrating peptide and an active ingredient; and its use. Specifically, a conjugate including a cell penetrating peptide which is a peptide comprising any one amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 156, a fragment of any one sequence of SEQ ID NO: 1 to SEQ ID NO: 156, or a peptide having above 80% homology with the above-mentioned sequence; and a composition comprising the same are disclosed.

## Background
REFERENCE TO SEQUENCE LISTING SUBMITTED ELECTRONICALLY
The content of the electronically submitted sequence listing (Name 24730810005_SUB_SEQ_LIST.txt; Size: 49,743 bytes; and Date of Creation: Jul. 8, 2015) filed with the application is incorporated herein by reference in its entirety.
FIELD OF THE INVENTION
The present invention relates to cell penetrating peptides derived from human telomerase reverse transcriptase (hTERT) enzyme, conjugates of the cell penetrating peptides, active ingredients, and compositions comprising the conjugate.
BACKGROUND
Although Low-molecular weight substances, nucleic acids, proteins, nano-particles, etc, have great potentials as therapeutic substances at a molecular level, their uses are limited due to the incompetence to penetrate into tissues and cell membrane. The development of a system to deliver such substances into the cell has been the active area of research over the last two decades. transport the substances inside the cell has been a conversation topic in a treatment of molecular method. Low-molecular weight substances, nucleic acids or nano-particles were transported inside the cell by several reagents, electroporation or heatshock. However, it was difficult to find an adequate method of delivery of proteins inside the cell without disrupting the activity and integrity of proteins. In 1980s, in the research conducted on studying the cell penetrating capacity of HIV, it was found that HIV-TAT protein consisting of specific 11 amino acids play an important role in a process of transportation inside the cell. Thus, in 1990s, studies on finding the right method of transporting proteins inside the cell has been the intense area of research.
Telomere is known as a repetitive sequence of genetic material found at the ends of chromosomes that prevent chromosomes from damage or merging onto other chromosomes. The length of the telomere is shortened at each cell division, and after a certain number of cell division, the telomere length is extremely shortened to the extent in which the cell stops dividing and dies. On the other hand, the elongation of telomeres is known to extend the life span of a cell. For an example, cancer cells excrete an enzyme called telomerase, which prevents shortening of telomeres, thus resulting in proliferation of cancer cells.
The objective of this invention is to provide a novel peptide.
Another objective of present invention is to provide the polynucleotide that codes the novel peptide.
Another objective of present invention is to provide a cell penetrating peptide.
Another objective of present invention is to provide a useful peptide as a carrier of the active ingredient in a cell.
Another objective of present invention is to provide a conjugate that an active ingredient and cell penetrating peptide are conjugated.
Another objective of present invention is to provide a composition comprising a conjugate of an active ingredient and cell penetrating peptide.
Another objective of present invention is to provide a pharmaceutical composition comprising a conjugate of an active ingredient and cell penetrating peptide.
Another objective of present invention is to provide a functional cosmetic composition comprising a conjugate of an active ingredient and cell penetrating peptide.
Another objective of present invention is to provide a health food composition comprising a conjugate of an active ingredient and cell penetrating peptide.
Another objective of present invention is to provide a contrast agent comprising a conjugate of an active ingredient and cell penetrating peptide.
SUMMARY OF THE INVENTION
The conjugate according to the one embodiment of the present invention may be a conjugate of cell penetrating carrier peptide and active ingredients, wherein the carrier peptide is the peptide comprising any one amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 156, the peptide having above 80% homology of amino acid sequence with above-mentioned sequences, or the fragment of the above-mentioned peptides, and wherein the peptide having above 80% homology of amino acid sequence with above-mentioned sequences and the fragment of the same are the peptides that maintain cell penetrating ability of any one amino acid sequences of 1 to SEQ ID NO: 156.
According to another embodiment of the conjugate in the present invention, the fragment may be made of 3 or more amino acids.
According to another embodiment of the conjugate in the present invention, the carrier peptide may be made of 30 or less amino acids.
According to another embodiment of the conjugate in the present invention, the above-mentioned carrier peptide may be the peptide having any one or more amino acid sequences of SEQ ID NO: 1 to SEQ ID NO: 156.
According to another embodiment of the conjugate in the present invention, which comprises any one amino acid sequence selected from the group consisting of: SEQ ID NO: 9, SEQ ID NO: 37, SEQ ID NO: 43, SEQ ID NO: 48, SEQ ID NO: 52, SEQ ID NO: 55, SEQ ID NO: 90, SEQ ID NO: 92 and SEQ ID NO: 121.
The contrast agent according to the one embodiment of the present invention may comprise any one conjugate above-mentioned.
The contrast agent according to the one embodiment of the present invention may be for contrasting a cell.
According to another embodiment of the contrast agent in the present invention, the cell may be a stem cell.
The composition according to one embodiment of the present invention may comprise any one of conjugates above-mentioned.
According to another embodiment of the composition in the present invention, the active ingredient may be for treatment or prevention of disease, and the composition may be pharmaceutical composition.
According to another embodiment of the composition in the present invention, the active ingredient may be the active ingredient for functional cosmetics, and the composition may be cosmetic composition.
According to another embodiment of the composition in the present invention, the active ingredient may be the active ingredient for functional health food, and the composition may be health food composition.
The cytoplasm targeting delivery system of active ingredient according to the one embodiment of the present invention may comprise any one of conjugates mentioned above, wherein the carrier peptide moves into a cytoplasm locally and performs a role of local cytoplasm delivering the mentioned active ingredients, wherein the peptide having above 80% homology of amino acid sequence with above-mentioned sequence and the fragment of the same are the peptides that maintain cell penetrating ability of any one amino acid sequences of SEQ ID NO: 1 to SEQ ID NO: 156.
According to another embodiment of the method in the present invention, the method may be for delivering the active ingredient locally into mitochondria inside a cell.
According to another embodiment of the cell penetrating peptide in the present invention, the above-mentioned carrier peptide may be the peptide having any one or more amino acid sequences of SEQ ID NO: 1 to SEQ ID NO: 156.
The polynucleotide according to the present invention may encode above-mentioned cell penetrating peptide.
The vector according to the present invention may comprise above-mentioned polynucleotide.
The transformed cell according to the present invention may comprise above-mentioned vector.
INDUSTRIAL APPLICABILITY
Active ingredients which are difficult to be transported inside a cell can be easily transported inside a cell by using the peptide, or the conjugate of the peptide and active ingredients, disclosed in the present invention. This means that efficacy of active ingredients can be increased and therefore the dosage can be lowered. As a result, side effects due to a drug administration can be minimized and effectiveness of treatment can be increased. Especially, as delivering drugs locally into mitochondria, mitochondria related diseases or disorders can be improved, and the effectiveness of prophylaxis and treatment of diseases can be increased. In a case of cosmetics, with a small amount of active ingredients, it can create an outstanding effect. By conjugating a peptide with a contrast substance, it can be used as a contrast substance to monitor a process of cell transplantation or transplanted cells in a cell treatment. Especially, it can be effectively used as a contrast substance for stem cells injected within a body.



BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 to FIG. 23 represents illustrating that the cellular uptake of the cell numbers from a HeLa cell treated with after the FITC fused to the peptide of SEQ: 1 to SEQ ID NO: 156, and analyzed by FACS. The control cells were treated only FITC.
FIG. 24 to FIG. 43 represents illustrating that the cellular uptake of the cell numbers from a Hurl cell treated with after the FITC fused to the peptide of SEQ: 1 to SEQ ID NO: 156, and analyzed by FACS. The control cells were treated only FITC.
FIG. 44 to FIG. 58 represents illustrating that the cellular uptake of the cell numbers from a human T lymphocyte cell line (Jurket) treated with after the FITC fused to the peptide of SEQ: 1 to SEQ ID NO: 156, and analyzed by FACS. The control cells were treated only FITC.
FIG. 59 to FIG. 72 represents illustrating that the result of toxicity and cell viability from a HeLa cell treated with after the FITC fused to the peptide of SEQ: 1 to SEQ ID NO: 156, and analyzed by flow cytometry (Flow cytometry). The control cells were treated only FITC.



DETAILED DESCRIPTION OF THE INVENTION
Proteins, Nucleic acids, Peptides or virus etc. have big potentials to be used as therapeutic substances. However, their uses are limited because they cannot penetrate tissues and cell membrane due to molecular level sizes. Although, the size of molecules is small, they cannot penetrate lipid-bilayer due to structure or characteristics of the molecules. Thus, through the use of electroporation, heat shock, etc., there were attempts to transport proteins, nucleic acids, peptides or viruses inside the cell; it was difficult to transfer those without neither damaging cell membrane nor keeping the active states of above molecules. There have been many studies conducted since TAT (Trans-Activating Transcriptional activator) protein derived from HIV (Human Immuno-deficiency Virus) has shown to work as cell penetrating peptide which can transport huge active substances inside the cell. Specifically, there have been studies conducted about substances that can transport huge molecules such as proteins, nucleic acids, peptides or virus inside the cell without causing any toxicity, unlike TAT protein which causes toxicity inside the cell. Therefore, the present invention was completed as present inventors have found that peptides derived from telomerase have outstanding efficacy as cell penetrating peptide without a noticeable toxicity.
Peptides are disclosed in SEQ ID NO: 1 to SEQ ID NO: 156 shown in Table 1 below. SEQ ID NO: 157 is a full length sequence of the Human telomerase protein. The “name” in Table 1 below was used for distinction of peptides. In a different specific embodiment of the present invention, more than one peptide of the mentioned peptides in SEQ ID NO: 1 to SEQ ID NO: 156 comprise a “synthetic peptide”, a synthesized peptide of selected areas of the telomerase. In the present specification, the term “pep” herein relates to a peptide that has any one amino sequence among SEQ ID NO: 1 to SEQ ID NO: 156, or a peptide comprising of amino acid sequence above 80% homology with above-mentioned sequences, or a peptide fragment of above-mentioned peptides.











TABLE 1





SEQ ID

Position in




NO
NAME
Telomerase
Sequence
Length







  1.
pep2
[660-689]
ALFSVLNYERARRPGLLGAS
30 aa





VLGLDDIHRA



 


  2.
pep3
[663-677]
SVLNYERARRPGLLG
15 aa


 


  3.
pep4
[674-683]
PGLLGASVLGL
10 aa


 


  4.
pep5
[615-624]
ALLTSRLRFI
10 aa


 


  5.
pep6
[613-621]
RPALLTSRL
 9 aa


 


  6.
pep7
[653-661]
RLTSRVKAL
 9 aa


 


  7.
pep8
[691-705]
RTFVLRVRAQDPPPE
15 aa


 


  8.
pep9
[653-667]
RLTSRVKALFSVLNY
15 aa


 


  9.
pep10
[651-665]
AERLTSRVKALFSVL
15 aa


 


 10.
pep11
[667-675]
YERARRPGL
 9 aa


 


 11.
pep12
[675-683]
LLGASVLGL
 9 aa


 


 12.
pep13
[680-689]
VLGLDDIHRA
10 aa


 


 13.
pep14
[677-686]
GASVLGLDDI
10 aa


 


 14.
pep15
[660-669]
ALFSVLNYER
10 aa


 


 15.
pep16
[663-672]
SVLNYERARR
10 aa


 


 16.
pep17
[679-688]
SVLGLDDIHR
10 aa


 


 17.
pep18
[662-671]
FSVLNYERAR
10 aa


 


 18.
pep19
[665-675]
NYERARRPGL
10 aa


 


 19.
pep20
[667-676]
YERARRPGLL
10 aa


 


 20.
pep21
[672-681]
RPGLLGASVL
10 aa


 


 21.
pep22
[668-676]
ERARRPGLL
 9 aa


 


 22.
pep23
[680-688]
VLGLDDIHR
 9 aa


 


 23.
pep24
[663-671]
SVLNYERAR
 9 aa


 


 24.
pep25
[664-672]
VLNYERARR
 9 aa


 


 25.
pep26
[670-678]
ARRPGLLGA
 9 aa


 


 26.
pep27
[673-681]
PGLLGASVL
 9 aa


 


 27.
pep28
[671-679]
RRPGLLGAS
 9 aa


 


 28.
pep29
[660-668]
ALFSVLNYE
 9 aa


 


 29.
pep30
[674-682]
GLLGASVLG
 9 aa


 


 30.
pep31
[679-687]
SVLGLDDIH
 9 aa


 


 31.
pep32
[668-675]
ERARRPGL
 8 aa


 


 32.
pep33
[670-677]
ARRPGLLG
 8 aa


 


 33.
pep34
[674-681]
GLLGASVL
 8 aa


 


 34.
pep35
[669-676]
RARRPGLL
 8 aa


 


 35.
pep36
[676-683]
LGASVLGL
 8 aa


 


 36.
pep37
[563-577]
VTETTFQKNRLFFYR
15 aa


 


 37.
pep38
[573-587]
LFFYRKSVWSKLQSI
15 aa


 


 38.
pep39
[583-597]
KLQSIGIRQRLKRYQ
15 aa


 


 39.
pep40
[603-617]
EAEVRQHREARPALL
15 aa


 


 40.
pep41
[613-627]
RPALLTSRLRFIPKP
15 aa


 


 41.
pep42
[623-637]
FIPKPDGLRPIVNMD
15 aa


 


 42.
pep43
[643-657]
RTFRREKRAERLTSR
15 aa


 


 43.
pep45
[683-697]
LDDIHRAWRTFVLRV
15 aa


 


 44.
pep46
[693-707]
FVLRVRAQDPPPELY
15 aa


 


 45.
pep47
[721-735]
PQDRLTEVIASIIKP
15 aa


 


 46.
pep48
[578-592]
KSVWSKLQSIGIRQH
15 aa


 


 47.
pep49
[593-608]
LKVQLRELSEAEVRQ
16 aa


 


 48.
pep50
 [1-20]
MPRAPRCRAVRSLLRSHYRE
20 aa


 


 49.
pep51
[21-40]
VLPLATEVRRLGPQGWRLVQ
20 aa


 


 50.
pep52
[41-60]
RGDPAAFRALVAQCLVCYPW
20 aa


 


 51.
pep53
[61-80]
DARPPPAAPSFRQVSCLKEL
20 aa


 


 52.
pep54
 [81-100]
VARVIARLCERGAKNV1AFG
20 aa


 


 53.
pep55
[101-120]
FALLDGARGGPPEAFTTSVR
20 aa


 


 54.
pep56
[121-140]
SYLPNTVTDALRGSGAWGLL
20 aa


 


 55.
pep57
[141-160]
LRRVGDDVLVHLLARCALEV
20 aa


 


 56.
pep58
[161-180]
LVAPSCAYQVCGPPLYQLGA
20 aa


 


 57.
pep59
[181-200]
ATQARPPPHASGPRRRLGCE
20 aa


 


 58.
pep60
[201-220]
RAWNHSVREAGVPLGLPAPG
20 aa


 


 59.
pep61
[221-240]
ARRRGGSASRSLPLPKRPRR
20 aa


 


 60.
pep62
[241-260]
GAAPEPERTPVGQGSWAHPG
20 aa


 


 61.
pep63
[261-280]
RTRGPSDRGFCVVSPARPAE
20 aa


 


 62.
pep64
[281-300]
EATSLEGALSGTRHSHPSVG
20 aa


 


 63.
pep65
[301-320]
RQHHAGPPSTSRPPRPWDTP
20 aa


 


 64.
pep66
[321-340]
CPPVYAETKHFLYSSGDKEQ
20 aa


 


 65.
pep67
[341-360]
LRPSELLSSLRPSLTGARRL
20 aa


 


 66.
pep68
[361-380]
VETIFLGSRPWMPGTPRRLP
20 aa


 


 67.
pep69
[381-400]
RLPQRYWQMRPLFLELLGNH
20 aa


 


 68.
pep70
[401-420]
AQCPYGVLLKTHCPLRAAVT
20 aa


 


 69.
pep71
[421-440]
PAAGVCAREKPQGSVAAPEE
20 aa


 


 70.
pep72
[441-460]
EDTDPRRLVQLLRQHSSPWQ
20 aa


 


 71.
pep74
[481-500]
RHNERRFLRNTKKFISLGKE
20 aa


 


 72.
pep75
[501-520]
AKLSLQELIWKMSYRDCAWL
20 aa


 


 73.
pep76
[521-540]
RRSPGVGCVPAAEHRLREEI
20 aa


 


 74.
pep77
[541-560]
LAKFLHWLMSVYVVELLRSF
20 aa


 


 75.
pep78
[561-580]
FYVTETTFQKNRLFEYRKSV
20 aa


 


 76.
pep79
[581-600]
WSKLQSIGIRQHLKRVQLRE
20 aa


 


 77.
pep80
[601-620]
LSEAEVRQHREARPALLTSR
20 aa


 


 78.
pep81
[621-640]
LREIPKPDGLRPIVNMDYVV
20 aa


 


 79.
pep82
[641-660]
GARTFRREKRAERLTSRVKA
20 aa


 


 80.
pep83
[661-680]
LFSVLNYERARRPGLLGASV
20 as


 


 81.
pep85
[701-720]
DPPPELYFVKVDVTGAYDTI
20 aa


 


 82.
pep86
[721-740]
PQDRLTEVIASIIKPQNTYC
20 aa


 


 83.
pep87
[741-760]
VRRYAVVQKAAHGHVRRAFK
20 aa


 


 84.
pep88
[761-780]
SHVSTLTDLQPYMRQFVAHL
20 aa


 


 85.
pep89
[781-800]
QETSPLRDAVVIEQSSSLNE
20 aa


 


 86.
pep90
[801-820]
ASSGLEDVFLRFMCHHAVRI
20 aa


 


 87.
pep91
[821-840]
RGKSYVQCQGIPQGSILSTL
20 aa


 


 88.
pep92
[841-860]
LCSLCYGDMENKLFAGIRRD
20 aa


 


 89.
pep93
[861-880]
GLLLRLVDDFLLVTPHLTHA
20 aa


 


 90.
pep94
[881-900]
KTFLRTLVRGYPEYGCVVNL
20 aa


 


 91.
pep95
[901-920]
RKTVVNFPVEDEALGGTAFV
20 aa


 


 92.
pep96
[921-940]
QMPAHGLFPITGLLLDTRTL
20 aa


 


 93.
pep97
[941-960]
EVQSDYSSYARTSIRASLTF
20 aa


 


 94.
pep99
 [981-1000]
KCHSLFLDLQVNSLQTVCTR
20 aa


 


 95.
pep100
[1001-1020]
IYKILLLQAYRFHACVLQLP
20 aa


 


 96.
pep101
[1021-1040]
FHQQVWKNPTFFLRVISDTA
20 aa


 


 97.
pep102
[1041-1060]
SLCYSILKAKNAGMSLGAKG
20 aa


 


 98.
pep103
[1061-1080]
AAGPLPSEAVQATCHQAFLL
20 aa


 


 99.
pep104
[1081-1100]
KLTRHRYTYVPLLGSLRTAQ
20 aa


 


100.
pep105
[1101-1120]
TQLSRKLPGTTLTALEAAAN
20 aa


 


101.
pep106
[1121-1132]
PALPSDFKTILD
12 aa


 


102.
pep107
 [1-10]
MPRAPRCRAV
10 aa


 


103.
pep108
[11-30]
RSLLRSHYREVLPLATFVRR
20 aa


 


104.
pep109
[31-50]
LGPQGWRLVQRGDPAAFRAL
20 aa


 


105.
pep110
[51-70]
VAQCLVCVPWDARPPPAAPS
20 aa


 


106.
pep111
[71-90]
FRQVSCLKELVARVLQRLCE
20 aa


 


107.
pepll2
 [91-110]
RGAKNYLARGFALIDGARGG
20 aa


 


108.
pep113
[111-130]
PPEAFTTSVRSYLPNTVTDA
20 aa


 


109.
pep114
[131-150]
LRGSGAWGLLLRRVGDDVLV
20 aa


 


110.
pep115
[151-170]
HLLARCALFVLVAPSCAYQV
20 aa


 


111.
pep116
[171-190]
CGPPLYQLGAATQARFPPHA
20 aa


 


112.
pe0117
[191-210]
SGPRRRLGCERAWNHSVREA
20 aa


 


113.
pep118
[211-230]
GVPLGLPAPGARRRGGSASR
20 aa


 


114.
pep119
[231-250]
SLPLPKRPRRGAAPEPERTP
20 aa


 


115.
pep120
[251-270]
VGQGSWAPPGRTRGPSDRGF
20 aa


 


116.
pep121
[271-290]
CVVSPARPAEEATSLEGALS
20 aa


 


117.
pep122
[291-310]
GTRHSHPSVGRQHPAGPPST
20 aa


 


118.
pep123
[311-330]
SRPPRPWDTPCPPVYAETKH
20 aa


 


119.
pep124
[331-350]
FLYSSGDKEQLRPSFLLSSL
20 aa


 


120.
pep125
[351-370]
RPSLTGARRLVETIFLGSRP
20 aa


 


121.
pep126
[371-390]
WMPGTPRRLPRLPQRYWQMR
20 aa


 


122.
pep127
[391-410]
PLFLELLGNHAQCPYGVLLK
20 aa


 


123.
pep128 
[411-430]
THCPLRAAVTPAAGVCAREK
20 aa


 


124.
pepl29
[431-450]
PQGSVAAPEEEDTDPRRLVQ
20 aa


 


125.
pep130
[451-470]
LLRQHSSPWQVYGEVRACLR
20 aa


 


126.
pep131
[471-490]
RLVPPGLWGSRPNERRFLRN
20 aa


 


127:
pep132
[491-510]
TKKFISLGKHAKLSLQELTW
20 aa


 


128.
pep133
[511-530]
KMSVRDCAWLRRSPGVGCVP
20 aa


 


129.
pep134
[531-550]
AAEHRLREEILAKFLHWLMS
20 aa


 


130.
pep135
[551-570]
VYVVELLRSFEYVTETTFQR
20 aa


 


131.
pep136
[571-590]
NRLFFYRKSVWSKLQSIGIR
20 aa


 


132.
pep137
[591-610]
QRLKRVQLRELSEAEVRQPR
20 aa


 


133.
pep138
[611-630]
EARPALLTSRLRFIPKPDGL
20 aa


 


134.
pep139
[631-650]
RPIVNMDYVVGARTPRREKR
20 aa


 


135.
pep140
[651-670]
AERLTSRVKALFSVLNYERA
20 aa


 


136.
pep141
[671-690]
RRPGLLGASVLGLDDIHRAW
20 aa


 


137.
pep142
[691-710]
RTFVLRVRAQDPPPELYPVK
20 aa


 


138.
pep143
[711-730]
VDVTGAYDTIPQDRLTEVLA
20 aa


 


139.
pep145
[751-770]
AHGhVRKAFKSHVSTLTDLQ
20 aa


 


140.
pep146
[771-790]
PYMRQFVAHLQETSPLRDAV
20 aa


 


141.
pep147
[791-810]
VIEQSSSLNEASSGLFDVEL
20 aa


 


142.
pep148
[811-830]
RFMCHHAVRIRGKSYVQCQG
20 aa


 


143.
pep149
[831-850]
IPQGSILSTLLCSLCYGDME
20 aa


 


144.
pep150
[851-870]
NKLFAGIRRDGLLLRLVDDF
20 aa


 


145.
pcp151
[871-890]
LLVTPHLTHAKTFLRTLVRG
20 aa


 


146.
pep152
[891-910]
VPLYGCVVNLRKTVVNFEVE
20 aa


 


147.
pep153
[911-930]
DEALGGTAFVQMPAHGLFEW
20 aa


 


148.
pep154
[931-950]
CGLLLDTRTLEVQSDYSSYA
20 aa


 


149.
pep156
[971-990]
RRKLFGVLRLKCHSLFLDLQ
20 aa


 


150.
pep157
 [991-1010]
VNSLQTVCTNIYKILLLQAY
20 aa


 


151.
pep158
[1011-1030]
RFHACVLQLPEHQQVWKNET
20 as


 


152.
pep159
[1031-1050]
FFLRVISDTASLCYSILKAK
20 aa


 


153.
pep160
[1051-1070]
NAGMSLGAKGAAGPLPSEAV
20 aa


 


154.
pep161
[1071-1090]
QWLCHQAPILKLTRHRVTYV
20 aa


 


155.
pep162
[1091-1110]
PLLGSLRTAQTQLSRKLEGT
20 aa


 


156.
pep163
[1111-1132]
TLTALEAAANPALPSDFKTI
22 aa





LD



 


157.
Telo-
   [1-1132]
MPRAPRCRAVRSLLRSEREY
1132



merse

LPLATHRRLGEQGWRLVQRG






DPAAFRALVAQCLVCVPWDA






RPPPAAPSFRQVSGLKELVA






RVLQRLCERGAKNVLAFGEA






LLDGARGGPPEAFTTSVRSY






LENIVTDALRGSGAWGLLLR






RVGDDVLWILLARCALFVLV






APSCAYQVCGPPLYQLGAAT






QARPEEHASGRRRLGCERAW






NHSVREAGVPLGLPAPGARR






RGGSASRSLPLPKRPRRGAA






PEPERTPVGQGSWAHPGRTR






GRSDRGFCVVSFARPAERAT






SLECALSGTRHSHPSVGRWH






AGPPSTSRPERPIVDTECPP






VYAETKHFLYSSGDREQLRP






SELLSSLRPSLTGARRLVET






IFLGSRPMEGTPRRLPRLPQ






RYWQMRPLFLELLGNHAQCP






YGVLLKTHCPLRAAVTPAAC






VCAREKPQGSVAAPEEEDTD






PRITLVQLLRQMSSPWQVYG






EVRACLRRLVITGLIMSENE






RRELRNTKKFISLGKLIAKL






SLQELTWKMSVRDCAWLRRS






EGVGCVPAARHRLREEILAK






FLHWLMSVYVVELLRSFEYV






TETTFQKNRLFFYRKSVWSK






LQSIGIRQHLKRVQLRELSE






AFVRQHREARPALLTSRLRF






IPKPDGLRPIVNMDYVVGAR






TFERERRAERLTSRVRALFS






VLNYERARRPGLLGASVLGL






DDIHRAWRTFVLRVRAQDEP






PELVFVKVDVTGAYDTIPUR






LTEVIASIIKPQNTYCVRRY






AVVQKAAHGHVRKAFKSHVS






TLTDLQEVMRQFVAHLQFTS






PLRDAVVJEQSSSLNEASSG






LFDVELRFMCHHAVRIRGKS






YVQCQGIPQGSILSTLLCSL






CYGDMENKLFAGIRRDGLLL






RLVDDFLLVTPHLTHAKTFL






RTLVRGVPEYGCVVNLRKTV






VNFPVEDEALGGTAFVQMPA






HGLFPWCGLLLDTRTLEVQS






DYSSYARTSIRASLTFNRGF






EAGRNMRRKLEGVLRLKCHS






LELDLQVNSLQTVCTNIYKI






LLWAYRFHACVLQLPFHQQV






WKNPTFFLRVISDTASLCYS






ILKAKNAGMSLGAKGAAULP






SEAVQWLCMQAFLLKLTRHR






VTYVPLLGSLRTAQTQLSRK






LPGTTLTALEAAANPALPSD






EKTILD



 


164 
pep1
[611-626]
EARPALLTSRLRFIEK
16 aa









In one embodiment of the present invention the polynucleotide codes a peptide comprising at least one amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 156, a peptide having above 80% homology with above-mentioned sequences, or a peptide being a fragment of the above-mentioned peptides. The polynucleotide mentioned above enables production of the peptides in large quantities. For example, cultivation of vectors that include polynucleotides encoding peptides allows production of peptides in large quantities.
The peptides disclosed herein can include a peptide comprising amino acid sequence above 80%, above 85%, above 90%, above 95%, above 96%, above 97%, above 98%, or above 99% homology. Moreover, the peptides disclosed in the present invention can include a peptide comprising any one amino sequence among SEQ ID NO: 1 to SEQ ID NO: 156 or its fragments, and a peptide with more than 1 transformed amino acid, more than 2 transformed amino acid, more than 3 transformed amino acid, more than 4 transformed amino acid, more than 5 transformed amino acid, more than 6 transformed amino acid, or more than 7 transformed amino acid.
In one embodiment of the present invention, changes in amino acid sequence belong to the modification of peptide's physical and chemical characteristics. For example, amino acid transformation can be performed by improving thermal stability of the peptide, altering substrate specificity, and changing the optimal pH.
The term “amino acid” herein includes not only the 22 standard amino acids that are naturally integrated into peptide but also the D-isomers and transformed amino acids. Therefore, in a specific embodiment of the present invention, a peptide herein includes a peptide having D-amino acids. On the other hand, a peptide may include non-standard amino acids such as those that have been post-translationally modified. Examples of post-translational modification include phosphorylation, glycosylation, acylation (including acetylation, myristoylation, palmitoylation), alkylation, carboxylation, hydroxylation, glycation, biotinylation, ubiquitinylation, transformation in chemical properties (e.g. β-removing deimidation, deamidation) and structural transformation (e.g. formation of disulfide bridge). Also, changes of amino acids are included and the changes of amino acids occur due to chemical reaction during the combination process with crosslinkers for formation of a peptide conjugate.
A peptide disclosed herein may be a wild-type peptide that has been identified and isolated from natural sources. On the other hand, when compared to peptide fragments of any one amino sequence among SEQ ID NO: 1 to SEQ ID NO: 156, the peptides disclosed herein may be artificial mutants that comprise one or more substituted, deleted and/or inserted amino acids. Amino acid alteration in wild-type polypeptide—not only in artificial mutants—comprises conservative substitution of amino acids that do not influence protein folding and or activation. Examples of conservative substitution belong to the group consisting of basic amino acids (arginine, lysine and histidine), acidic amino acids (glutamic acid and aspartic acid), polar amino acids (glutamine and asparagines), hydrophobic amino acids (leucine, isoleucine, valine and methionine), aromatic amino acids (phenylalanine, tryptophan and tyrosine), and small amino acids (glycine, alanine, serine, and threonine). The amino acid substitutions that do not generally alter the specific activity are known in the art of the present invention. Most common occurred alteration are Ala/Ser, Val/Ile, Asp/Glu, Thr/Ser, Ala/Gly, Ala/Thr, Ser/Asn, Ala/Val, Ser/Gly, Tyr/Phe, Ala/Pro, Lys/Arg, Asp/Asn, Leu/Ile, Leu/Val, Ala/Glu, Asp/Gly, and the opposite alterations. Another example of conservative substitutions are shown in the following table 2.









TABLE 2





Original amino

Preferable residue


acid
Examples of residue substitution
substitution







Ala (A)
val; leu; ile
Val


Arg (R)
lys; gln; asn
Lys


Asn (N)
gln; his; asp, lys; arg
Gln


Asp (D)
glu; asn
Glu


Cys (C)
ser; ala
Ser


Gln (Q)
asn; glu
Asn


Glu (E)
asp; gln
Asp


Gly (G)
ala
Ala


His (H)
asn; gln; lys; arg
Arg


Ile (I)
leu; val; met; ala; phe; norleucine
Leu


Leu (L)
norleucine; ile ; val; met; ala; phe
Ile


Lys (K)
arg; gln; asn
Arg


Met (M)
leu; phe; ile
Leu


Phe (F)
leu; val; ile; ala; tyr
Tyr


Pro (P)
ala
Ala


Ser (S)
thr
Thr


Thr (T)
ser
Ser


Trp (W)
tyr; phe
Tyr


Tyr (Y)
trp; phe; thr; ser
Phe


Val (V)
ile; leu; met; phe; ala; norleucine
Leu









The substantial transformation of the biological properties of peptides are performed by selecting significantly different substitution in the following efficacies: (a) the efficacy in maintaining the structure of the polypeptide backbone in the area of substitution, such as sheet or helical three-dimensional structures, (b) the efficacy in maintaining electrical charge or hydrophobicity of the molecule in the target area, or (c) the efficacy of maintaining the bulk of the side chain. Natural residues are divided into groups by general side chain properties as the following:

    (1) hydrophobicity: Norleucine, met, ala, val, leu, ile;
    (2) neutral hydrophilicity: cys, ser, thr;
    (3) acidity: asp, glu;
    (4) basicity: asn, gln, his, lys arg;
    (5) residue that affects chain orientation: gly, pro; and
    (6) aromaticity: trp, tyr, phe.


Non-conservative substitutions may be performed by exchanging a member of the above classes to different classes. Any cysteine residues that are not related in maintaining the proper three-dimensional structure of the peptide can typically be substituted into serine, thus increasing the oxidative stability of the molecule and preventing improper crosslinkage. Conversely, improvement of stability can be achieved by adding cysteine bond(s) to the peptide.
Altered types of amino acids variants of peptides are those that antibody glycosylation pattern changed. The term “change” herein relates to deletion of at least one carbohydrate residues that are found in a peptide and/or addition of at least one glycosylated residues that do not exist within a peptide
Glycosylation in peptides are typically N-connected or O-connected. The term “N-connected” herein relates to that carbohydrate residues are attached to the side chain of asparagine residues. As tripeptide sequences, asparagine-X-serine and asparagine-X-threonine (where the X is any amino acid except proline) are the recognition sequence for attaching carbohydrate residue enzymatically to the side chain of asparagine. Therefore, with the presence of one of these tripeptide sequences in a polypeptide, the potential glycosylation sites are created. “O-connected glycosylation” means attaching one of sugar N-acetylgalactosamine, galactose, or xylose to hydroxyl amino acids. The hydroxyl amino acids are most typically serine or threonine, but 5-hydroxyproline or 5-hydroxylysine can be used.
Addition of glycosylation site to a peptide is conveniently performed by changing amino acid sequence to contain tripeptide sequence mentioned above (for N-linked glycosylation sites). These changes may be made by addition of at least one serine or threonine residues to the first antibody sequence, or by substitution with those residues (for O-linked glycosylation sites).
In one embodiment of the present invention, cell penetrating peptide comprising any one amino acid sequences of SEQ ID NO: 1 to SEQ ID NO: 156, a peptide having above 80% homology of amino acid sequence with above-mentioned sequences, or a fragment of the above-mentioned peptides, is provided.
In one embodiment of the present invention, a pharmaceutical composition comprising peptide as a drug delivery system to transport more than one active ingredient is provided, wherein the peptide comprises any one amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 156, the peptide has above 80% homology with above-mentioned sequence, or the peptide is a fragment of above-mentioned peptide.
A peptide comprising any one amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 156, a fragment of above-mentioned peptide, or a peptide having above 80% homology with above-mentioned sequence, is safe and has outstanding efficacy as cell penetrating peptide. Therefore, the peptide can be conjugated with a drug to transport the drug inside the cell.
In one embodiment of the present invention, a conjugate of a peptide and an active ingredient to be transported is provided, wherein the peptide comprises any one amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 156, the peptide is a fragment of above-mentioned peptide, or the peptide has above 80% homology with above-mentioned peptide. In one embodiment of the present invention, an active ingredient may be at least one selected from proteins, nucleic acids, peptides, lipids, glycolipids, minerals, sugars, contrast substances, drugs and chemical compounds. In one embodiment of the present invention, the active ingredients may be peptides. In one embodiment of the present invention, the active ingredients may be cytokines, antibody, antibody fragments, therapeutic enzymes, soluble receptors, or ligands.
A cell penetrating peptide disclosed herein means a peptide which can transport cargo from in vitro and/or in vivo to inside the cell. A “cargo” disclosed herein comprises all the substances that can be transported inside the cell via conjugation with a cell penetrating peptide, For example, all the substances which want to increase cell penetrating efficacy, specifically drugs, cosmetics, or active ingredients of health food, more specifically substances which cannot be transported inside the cell via general route, more specifically, sugars, nano-particles, biological formulation, viruses, contrast substances or other chemical compounds which can have proteins, nucleic acids, peptide, minerals, glucose as an example, but not limited to those. A “drug” disclosed herein is a broad concept including a substance to be transported for alleviation, prophylaxis, treatment or diagnosis of diseases, wounds, or specific symptom.
A “carrier peptide” disclosed herein is a peptide which can transport active ingredients to a targeted site via conjugation with active ingredients.
In one embodiment of the present invention, protein or peptide as a cargo comprises one or more of hormone, hormone analogue, enzyme, enzyme inhibitors, signal transfer proteins (or peptides), antibody and vaccine, but not limited to those. In one embodiment of the present invention, a nucleic acid is a molecule that can be spontaneous or artificial DNA or RNA molecules, either single-stranded or double-stranded. The nucleic acid molecule can be one or more nucleic acids of same type (for example, having a same nucleotide sequence) or nucleic acids of different types. The nucleic acid molecules comprise one or more DNA, cDNA, decoy DNA, RNA, siRNA, miRNA shRNA, stRNA, snoRNA, snRNA PNA, antisense oligomer, plasmid and other modified nucleic acids, but not limited to those. In one embodiment of the present invention, virus comprises the whole virus or the core of virus which includes nucleic acids of the virus. In one embodiment of the present invention, a chemical substance is a broad indication comprising a natural or synthetic substance which can act as a drug.
In one embodiment of the present invention, drug to be delivered into cells by a cell-permeable peptide is iposomes, micelles, nanoparticles, or quantum dots as drug delivery.
The term “contrast substance” disclosed herein is a broad indication comprising all the substances used to contrast structures or fluids within the body in medical imaging. An appropriate contrast substance comprises radiopaque contrast agent, paramagnetic contrast agent, superparamagnetic contrast agent, CT (computed tomography) and other contrast substances, but not limited to those. For example, a radiopaque contrast agent (for x-ray imaging) will comprise inorganic iodine compound and organic iodine compound (for example, diatrizoate), radiopaque metals and their salts (for example, silver, gold, platinum, etc.) and other radiopaque compounds (for example, calcium salts, barium salt such as barium sulfate, tantalum and oxidized tantalum). An appropriate paramagnetic contrast substance (for MR imaging) comprises gadolinium diethylene triaminepentaacetic acid (Gd-DTPA) and its derivatives, other gadolinium, manganese, iron, dysprosium, copper, europium, erbium, chrome, nickel and cobalt complex, for example, 1,4,7,10-tetraazacyclododecan-N,N′,N′′,N′′′-tetraacetic acid (DOTA), ethylenediaminetetraacetic acid (EDTA), 1,4,7,10-tetraazacyclododecan-N,—N′,N′′-triacetic acid (DO3A), 1,4,7-triazacyclononane-N,N′, N′′-TRIACETIC ACID (NOTA), 1,4,8,10-tetraazacyclotetradecane-N,N′,N′′,N′′′-tetraacetic acid (TETA), hydroxybenzylethylene-diamine diacetic acid (HBED). An appropriate superparamagnetic contrast substance (for MR imaging) comprises magnetite, superparamagnetic iron oxide (SPIO), ultrasmall superparamagnetic iron oxide (USPIO) and monocrystalline iron oxide. Other appropriate contrast substances are iodinated, non-iodinated, ionic and non-ionic CT contrast agents, a contrast substance like spin-label or diagnostically effective agent.
Other examples of contrast substances comprise β-galactosidase, Green Fluorescent Protein, Cyan Fluorescent Protein, luciferase, but not limited to those, and a marker gene which codes for protein which can be easily detected when expressed within cells. Various labels such as radionuclide, flour, enzyme, enzyme-substrate, enzyme cofactor, enzyme inhibitor, ligands (especially hapten) can be used.
In one example of the present invention, a contrast substance is ferrocenecarboxylic acid of the below chemical formula 2. The structure of ferrocene is shown in the chemical formula 1.




In one example of the present invention, a conjugate of cell penetrating peptide and a contrast substance is Ferrocenecarboxylic-pep (Ferrocenecarboxylic-pep) shown in the below chemical formula 3.




In one embodiment of the present invention, a peptide or composition can be fused with one or more detectable labels. Labels may be compounds which can be detected in chemical, physical or enzymatic responses, or compounds which generate signals directly or indirectly in the responses. Labeling and detecting after then can be performed according to the known method in the art (For example, Sambrook, J., and Russel, D. W. (2001); and Lottspeich, F., and Zorbas H. (1998) Bioanalytik, Spektrum Akademischer Verlag, Heidelberg/Berlin, Germany). Labels comprise fluorescent label, enzyme label, chromogenic label, luminescence label, radiation label, hapten, biotin, metal complex, metal and colloidal gold, but not limited to those. All forms of these labels are well known in this field of work, they can be commercially obtained from various suppliers.
In one embodiment of the present invention, a cargo can be directly combined with the peptide. In another embodiment of the present invention, a cargo can be combined to the peptide via various types of bonds such as covalent or non-covalent bonds. A cargo, for example, can be combined to the N-terminal or C-terminal of the peptide in one embodiment of the present invention. For example, a cargo can be bonded to the peptide by disulfide bonds or covalent bonds. The covalent bonds are the bonds that a cargo can be bonded to α-amine of N-terminal glutamate, or amine of C-terminal Lysine residues. Also, a peptide and a cargo can be combined via a non-covalent bond, which can have either a peptide or a cargo can encapsulate the other as a capsule form.
In another embodiment of the present invention, a peptide can be combined with a cargo via a linker. For example, a peptide can be combined with a cargo by binding a cargo to a linker after introducing a linker such as Hynic(6-hydrazinopyridine-3-carboxylic acid) linker, to the α-amine of N-terminal glutamate, or amine of C-terminal Lysine residues.
In another embodiment of the present invention, when a cargo is DNA or RNA, SH group (thiol group) is introduced to the peptide, and maleimide group is introduced to DNA or RNA, then, SH group of the peptide and maleimide group of DNA or RNA are combined, thus creating a bond between the cargo and the peptide.
In another embodiment of the present invention, when a cargo is a peptide or protein, DNA which expresses a cargo is combined with DNA which expresses a carrier peptide, and by expressing this, a cargo and a peptide can be combined as a form of fusion protein. Specific examples of combination by a fusion protein are as follows: when manufacturing primer for production of fusion protein, a nucleotide coding a carrier peptide is attached in front of a nucleotide expressing a cargo, and the obtained nucleotide is inserted to a vector such as pET vector using a restriction enzyme, and the nucleotide is expressed by transformation into a cell such as BL-21(DE3). At this time, a fusion protein is to be effectively expressed by treating it with an expression inducing agent like IPTG (isopropyl-1-thio-β-D-galactopyranoside). Then, the expressed fusion protein is purified by His tag purification, and is dialyzed with PBS, and is added to a kit to be concentrated by centrifugation under such condition for 5 to 20 mins at 2,000 to 4,000 rpm.
In one embodiment of the present invention, a carrier peptide is combined with dying substances, fluorescent substances, specifically FITC (fluorescein isothiocyanate) or GFP (Green Fluorescent Protein). In one embodiment of the present invention, FITC is combined with amino group (NH3+) of lysine at N-terminal or C-terminal of a carrier peptide. In the case of a peptide, where lysine does not exist at its terminal, the peptide can be combined with FITC via a linker including Lysine.
The carrier peptide disclosed herein which is the peptide comprising any one amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 156, or the peptide having above 80% homology of amino acid sequence with above-mentioned peptides, or a fragment of above-mentioned peptide, can be combined with a cargo at a mole fraction of 1:1, but it can be combined at mole fraction other than 1:1. For example, a mole fraction of CPP and a cargo may be more than 2:1, specifically, more than 2:1, more than 3:1, more than 4:1, more than 5:1, more than 6:1, more than 7:1, more than 8:1, more than 9:1 or more than 10:1. This means that numerous carrier peptide molecules can be combined with a cargo molecule. The numerous carrier peptide molecules can be combined in series or in parallel. “Combined in series” means that a carrier peptide and a cargo molecule are to be combined at terminal amino acids. “Combined in parallel” means that they are to be combined at a site other than terminal amino acids. On the other hand, the mole fraction of a carrier peptide and a cargo may be more than 1:2. This means that a carrier peptide molecule can be combined with numerous number of a cargo molecule. For example, a mole fraction of a carrier peptide and a cargo may be 1:2, specifically, more than 1:2, more than 1:3, more than 1:4, more than 1:5, more than 1:6, more than 1:7, more than 1:8, more than 1:9 or more than 1:10.
A movement pathway of the peptide combined with Fluorescein isothiocyanate can be easily found. Therefore, a carrier peptide in one embodiment of the present invention is to be used for cell imaging or detecting a pathway of drug delivery inside a cell.
In one embodiment of the present invention, a use of the peptide as a drug delivery carrier to transport more than one active ingredient is provided, wherein the peptide comprises any one amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 156, or the peptide is a fragment of above-mentioned peptide, or the peptide has above 80% homology of amino acid sequence with above-mentioned peptide. The use may indicate therapeutic or non-therapeutic use.
In one embodiment of the present invention, a method of delivering drugs inside a cell of a subject comprising a step of administering a composition comprising a drug; and the peptide is provided; wherein the peptide comprises any one amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 156, or the peptide is a fragment of above-mentioned peptide, or the peptide has above 80% homology of amino acid sequence with above-mentioned peptide.
In one embodiment of the present invention, a method of detecting drug delivery pathway comprising a step of applying the peptide and a contrast substance to a subject is provided; wherein the peptide comprises any one amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 156, or the peptide is a fragment of above-mentioned peptide, or the peptide has above 80% homology of amino acid sequence with above-mentioned peptide.
In one embodiment of the present invention, a method of detecting drug delivery pathway comprising a step of applying of the conjugate of the peptide and a contrast substance to a subject is provided; wherein the peptide comprises any one amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 156, or the peptide is a fragment of above-mentioned peptide, or the peptide has above 80% homology of amino acid sequence with above-mentioned peptide.
In one embodiment of the present invention, a kit for drug delivery into a cell of a subject containing the composition and an instruction is provided, wherein the composition comprises a conjugate of a peptide of the invention and a drug for delivery, wherein the peptide comprises any one amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 156 or the peptide is a fragment of above-mentioned peptide, or the peptide has above 80% homology of amino acid sequence with above-mentioned peptide, wherein the instruction includes at least one of administration dose, administration route, administration frequency, and indication of the composition.
In one embodiment of the present invention, cosmetic or food composition comprising an active ingredient; and the peptide is provided; wherein the peptide comprises amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 156, the peptide has above 80% homology of amino acid sequence with above-mentioned sequence, or the peptide is a fragment of the above-mentioned peptides. In another embodiment of the present invention, cosmetic or food composition comprising a conjugate of the peptide and active ingredients is provided; wherein the peptide comprises any one amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 156, the peptide has above 80% homology of amino acid sequence with above-mentioned sequence, or the peptide is a fragment of the above-mentioned peptides.
In one embodiment of the present invention, pharmaceutical, cosmetic or food composition with an outstanding ability to transport active ingredients inside a cell, comprising a conjugate of the peptide and an active ingredient, is provided; wherein the peptide comprises any one amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 156, the peptide has above 80% homology of amino acid sequence with above-mentioned sequence, or the peptide is a fragment of the above-mentioned peptides.
Mitochondria, as a central organelle in energy metabolism of a eukaryotic cell, is a first known intracellular organelle to be related to human diseases (Luft R, Ikkos D, Palmieri G, Ernster L, Afzelius B: A case of severe hypermetabolism of non thyroid origin with a defect in the maintenance of mitochondrial respiratory control: a correlated clinical, biochemical, and morphological study, J Clin Invest 41: 1776-804, 1962).
Since the mitochondria play an important role in control of energy metabolism of cell and apoptosis, they act as a major target for various therapeutic drugs. Also, this organelle is involved in control of the calcium concentration inside the cell, the mitochondrial respiratory chain acts as an electron transport system which is important in energy production, and it causes production of reactive oxygen species. As a result, the abnormal mitochondrial function has a close relationship with adult diseases such as diabetes, cardiomyopathy, infertility, blindness, renal/liver diseases, and stroke (Modica-Napolitano K S. Singh K K: April mitochondria as targets for detection and treatment of cancer. Expert Rev Mol Med 11:1-19, 2002). Also, it is being suggested that Mitochondrial genetic mutations to be involved in the outbreak of aging, degenerative neuronal disease and cancer etc.
The mitochondria targeting delivery system can be provided according to the one embodiment of the present invention may comprise any one of conjugates mentioned above, wherein the carrier peptide moves into intracellular mitochondria locally and performs a role of local intracellular mitochondria delivering the mentioned active ingredients, wherein the peptide having above 80% homology of amino acid sequence with above-mentioned sequence and the fragment of the same are the peptides that maintain mitochondria targeting delivery system, the above-mentioned mitochondria targeting peptide may be the peptide having any one amino acid sequences of SEQ ID NO: 1 to SEQ ID NO: 156.
The mitochondria activity adjusting composition can be provided, wherein the composition comprises a conjugate of a peptide of the invention and a carried peptide for delivery, wherein the carrier peptide moves into intracellular mitochondria locally and performs a role of local intracellular mitochondria delivering the mentioned active ingredients, wherein the peptide having above 80% homology of amino acid sequence with above-mentioned sequence and the fragment of the same are the peptides that maintain mitochondria targeting delivery system, the above-mentioned mitochondria targeting peptide may be the composition having any one amino acid sequences of SEQ ID NO: 1 to SEQ ID NO: 156.
The mitochondria activity adjusting composition according to the one embodiment of the present invention, wherein the composition for the treatment of a mitochondrial related diseases or disorder, prevention, inhibitory of progress, or relief of symptoms as an a pharmaceutical composition, wherein the active ingredient will be treated for a mitochondrial related diseases or disorder, prevention, inhibitory of progress, or relief of symptoms.
The “Mitochondrial related diseases” disclosed herein comprise Huntington's disease, amyotriophic lateral sclerosis, MELAS (Mitochondrial Encephalomyopathy with Lactic Acidemia and Stroke-like episodes); MERRF (Myoclonus, epilepsy, and myopathy with ragged red fibers; NARP/MILS (Neurogenic muscular weakness, ataxia, retinitis pigmentosa/Maternally inherited leigh syndrome); LHON (Lebers hereditary optic neuropathy); KSS (Kearns-Sayre Syndrome); PMPS (Pearson Marrow-Pancreas Syndrome); CPEO (Chronic progressive external opthalnoplegia); Reye's syndrome; Alper's syndrome; Multiple mtDNA deletion syndrome; mtDNA depletion syndrome; Complex I deficiency; Complex II (SDH) deficiency; Complex III deficiency; Cytochrome c oxidase (COX, Complex IV) deficiency; Complex V deficiency; Adenine nucleotide translocator (ANT) deficiency; Pyruvate dehydrogenase (PDH) deficiency; Ethyl malonic acid aciduria having lactic acid acidemia; 3-methyl glutaconic acid aciduria having lactic acid acidemia; refractoriness epilepsy representing a decline during infection; Asperger's syndrome representing a decline during infection; Autism representing a decline during infection; Attention deficit hyperactivity disorder (ADHD); Cerebral palsy representing a decline during infection; Alexia representing a decline during infection; Maternal hereditary thrombocytopenia; Leukemia; MNGIE (Mitochondrial myopathy, peripheral and autonomic neuropathy, gastrointestinal dysfunction, and epilepsy); MARIAHS syndrome (Mitochondrial ataxia, recrudescent infection, aphasia, hypouricemia/hypomyelination, seizure and dicarboxylic acid aciduria); ND6 dystonia; Cyclic vomiting syndrome representing a decline during infection; 3-hydroxyisobutyric acid aciduria having lactic acid acidemia; Diabetes having lactic acid acidemia; Uridine reactive neural syndrome (URNS); Familial bilateral striatum necrosis (FBSN); Hearing loss related with aminoglycoside; Relaxed myocardiopathy; Spleen lymphoma; Wolframs syndrome; Multiple mitochondria DNA deletions syndrome; and Renal tubular acidosis/diabetes/ataxia syndrome, but not limited to those.
In another embodiment of the present invention, nucleic acid molecules encoding above-mentioned polypeptides are provided. The nucleic acid molecules, for example, have base sequences of GAA GCG CGC CCG GCG CTG CTG ACC AGC CGC CTG CGC TTT ATT CCG AAA (SEQUENCE NO: 181). The nucleic acids can be introduced into the host cell according to a known method to those skilled in the art. For example, the known methods may be transformation method by calcium phosphate method, liposome, electroporation, contacting a virus and a cell, or micro injection directly into the cell, etc. The host cell is higher eukaryotic cell, for example, mammalian cells, or lower eukaryotic cells, such as a yeast cell, or prokaryotic cells, such as a bacterial cell. The prokaryotic host cells appropriate for transformation may be the species which belong to E. coli, Bacillus subtillis, Salmonella typhimurium, Pseudomonas, Streptomyces, and Micro bacteria species, as examples.
The vector including above-mentioned nucleic acid molecules is generally recombinant expression vector and it comprises, origin of replication enabling a host cell transformation, and a selectable marker (for example, dihydrofolate reductase for eukaryotic cell culture, or tolerance of neomycin, tolerance of tetra-cycline or ampicillin in E. coli, or S. cerevisiae TRP1 gene), and the promoter for controlling transcription of protein coating sequences. Available expression vectors are, for example, known bacterial plasmids such as SV40, derivatives of pcDNA, and known bacterial plasmids such as colE1, pCR1, pBR322, pMal-C2, pET, pGEX (Smith, et al., Gene 67:31-40 (1988)), plasmids such as pMB9 and its derivative RP4, phage DNA which is the same as numerous derivatives of phage I such as NM989, phage DNA such as M13 and single-stranded phage DNA of filament type; yeast plasmid, for example, phage DNA or vector induced from a combination of modified plasmid for using expression suppression sequences and phage DNA. The mammalian expression vectors comprise origin of replication, an appropriate promoter and an enhancer. Also, they can comprise compulsory ribosome binding sites, polyadenylation sites, splice donor, and receptor part, transcription termination sequences, and 5′ planking non-transcriptional sequences. The mammalian expression vectors can comprise an inducible promoter, for example, a vector containing dihydrofolate reductase promoter, any expression vectors containing DHFR expression cassette or DHFR/methotrexate co-amplification vector such as pED. (Randal J, kaufman, 1991, Randal J. Kaufman, Current Protocols in Molycular Biology, 16, 12(1991)). Or, glutamine synthetase/methionine sulfoximine co-amplification vector, for example, pEE14 (Celltech), Epstein-Barr-Virus (EBV), or a vector directing episomal expression under the control of nuclear antigen (EBNA), for example, pREP4 (Invitrogen), pCEP4 (Invitrogen), pMEP4 (Invitrogen), pREP8 (Invitrogen), pREP9 (Invitrogen) and pEBVH is (Invitrogen) can be used. Selectable mammalian expression vectors are Rc/CMV (Invitrogen) and pRc/RSV (Invitrogen) etc. Vaccinia virus mammalian expression vectors which can be used in the present invention are pSC11, pMJ601, pTKgptF1S, etc.
Yeast expression vector system to be used in the present invention is non-fusion pYES2 vector (Invitrogen), fusion pYESHisA, B, C (Invitrogen), pRS vector, etc.
The above-mentioned vectors can be introduced to various cells, such as mammalian cells which is especially the human derived cells, or bacteria, yeast, fungi, insects, nematodes, and plant cells. The examples of appropriate cells are VERO cell, HELA cell, for example, ATCC No. CCL2, CHO cell line, for example, ATCC No. CCL61, COS cell, for example COS-7 cell and ATCC No. CRL 1650 cell, W138, BHK, HepG2, 3T3, for example, ATCC No. CRL6361, A549, PC12, K562 cell, 293 cell, Sf9 cell, for example, ATCC No. CRL1711 and Cv1 cell, such as ATCC No. CCL70, etc.
Other appropriate cells to be used in the present invention are prokaryotic host cell strain, for example, the strains belonging to E. coli (e.g. DH5-α strain), Bacillus subtilis, Salmonella typhimurium, Pseudomonas, Streptomyces and Staphylococcus. 
In one embodiment of the present invention, the composition may contain 0.1 μg/mg to 1 mg/mg, specifically 1 μg/mg to 0.5 mg/mg, more specifically 10 μg/mg to 0.1 mg/mg of a peptide comprising any one amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 156, a peptide comprising amino acid sequence above 80% homology with above-mentioned sequence, or a fragment of above-mentioned peptide. When the peptide is contained in the above-mentioned range, all the safety and stability of the composition can be satisfied and appropriate in terms of cost-effectiveness.
In one embodiment of the present invention, the composition may have application with all animals including human, dog, chicken, pig, cow, sheep, guinea pig, and monkey.
In one embodiment of the present invention, the pharmaceutical composition may be administered through oral, rectal, transdermal, intravenous, intramuscular, intraperitoneal, in the bone marrow, epidural or subcutaneous means.
Forms of oral administration may be, but not limited to, tablets, pills, soft or hard capsules, granules, powders, solution, or emulsion. Forms of non-oral administration can be, but not limited to, injections, drips, lotions, ointments, gels, creams, suspensions, emulsions, suppository, patch, or spray.
In one embodiment of the present invention, the pharmaceutical composition, if necessary, may contain additives, such as diluents, excipients, lubricants, binders, disintegrants, buffers, dispersants, surfactants, coloring agents, aromatics or sweeteners.
In one embodiment of the present invention, the pharmaceutical composition may be manufactured by conventional methods of the industry in the art.
In one embodiment of the present invention, the active ingredient of the medical composition may vary according to the patient's age, sex, weight, pathology and state, administration route, or prescriber's judgment. Dosage based on these factors is determined within levels of those skilled in the art, and the daily dose for example may be, but not limited to, 0.1 μg/kg/day to 1 g/kg/day, specifically 1 μg/kg/day to 10 mg/kg/day, more specifically the 10 μg/kg/day to 1 mg/kg/day, more specifically the 50 μg/kg/day to 100 μg/kg/day. In one embodiment of the present invention, the pharmaceutical composition may be administered, but not limited to, 1 to 3 times a day.
In one embodiment of the present invention, cosmetic composition may be provided in all forms appropriate for topical applications. For example, forms may be provided as solutions, emulsions obtained by dispersion of oil phase in water, emulsion obtained by dispersion of water in oil phase, suspension, solid, gel, powder, paste, foam or aerosol. These forms may be manufactured by conventional methods of the industry in the art.
In one embodiment of the present invention, the cosmetic composition may include, within levels that won't harm the main effect, other ingredients that may desirably increase the main effect. In one embodiment of the present invention, the cosmetic composition may additionally include moisturizer, emollient agents, surfactants, UV absorbers, preservatives, fungicides, antioxidants, pH adjusting agent, organic or inorganic pigments, aromatics, cooling agent or antiperspirant. The formulation ratio of the above-mentioned ingredients may be decided by those skilled in the art within levels that won't harm the purpose and the effects of the present invention, and the formulation ratio based on total weight of the cosmetic composition may be 0.01 to 5% by weight, specifically 0.01 to 3% by weight.
In one embodiment of the present invention, food composition is not limited to forms, but for example may be granules, powder, liquid, and solid forms. Each form may be formed with ingredients commonly used in the industry appropriately chosen by those skilled in the art, in addition to the active ingredient, and may increase the effect with other ingredients.
Decision for dosage on the above-mentioned active ingredient is within the level of those skilled in the art, and daily dosage for example may be 1 μg/kg/day to 10 mg/kg/day, more specifically the 10 μg/kg/day to 1 mg/kg/day, more specifically the 50 μg/kg/day to 100 μg/kg/day, but not limited to these numbers and can vary according to age, health status, complications and other various factors.
The terms used herein is intended to be used to describe the embodiments, not to limit the present invention. Terms without numbers in front are not to limit the quantity but to show that there may be more than one thing of the term used. The term “including”, “having”, “consisting”, and “comprising” shall be interpreted openly (i.e. “including but not limited to”).
Mention of range of numbers is used instead of stating separate numbers within the range, so unless it is explicitly stated, each number can be read as separate numbers integrated herein. The end values of all ranges are included in the range and can be combined independently.
Unless otherwise noted or clearly contradicting in context, all methods mentioned herein can be performed in the proper order. The use of any one embodiment and all embodiment, or exemplary language (e.g., that use “like  ̃”), unless included in the claims, is used to more clearly describe the present invention, not to limit the scope of the present invention. Any language herein outside of the claims should not be interpreted as a necessity of the present invention. Unless defined otherwise, technical and scientific terms used herein have meaning normally understood by a person skilled in the art that the present invention belongs to.
The preferred embodiments of the present invention are the best mode known to the inventors to perform the present invention. It can become clear to those skilled in the art after reading the statements ahead of the variations in the preferred embodiments. The present inventors hope that those skilled in the art can use the variations adequately and present invention be conducted in other ways than listed herein. Thus, the present invention, as allowed by the patent law, includes equivalents, and variations thereof, of the key points of the invention stated in the appended claims. In addition, all possible variations within any combination of the above-mentioned components are included in the present invention, unless explicitly stated otherwise or contradicting in context. Although the present invention is described and shown by exemplary embodiments, those skilled in the art will understand well that there can be various changes in the form and details without departing from the spirit of the invention and range, defined by the claims below.
Example 1: Synthesis of Peptide
The peptides of SEQ ID NO: 1 to SEQ ID NO: 156 were synthesized according to the existing method of solid phase peptide synthesis. In detail, the peptides were synthesized by coupling each amino acid from C-terminus through Fmoc solid phase peptide synthesis, SPPS, using ASP48S (Peptron, Inc., Daejeon ROK). Those peptides with their first amino acid at the C-terminus being attached to resin were used as follows:

    NH2-Lys(Boc)-2-chloro-Trityl Resin
    NH2-Ala-2-chloro-Trityl Resin
    NH2-Arg(Pbf)-2-chloro-Trityl Resin


All the amino acid materials to synthesize the peptide were protected by Fmoc at the N-terminus, and the amino acid residues were protected by Trt, Boc, t-Bu (t-butylester), Pbf (2,2,4,6,7-pentamethyl dihydro-benzofuran-5-sulfonyl) that can be dissolved in acid. Such as:

    Fmoc-Ala-OH, Fmoc-Arg(Pbf)-OH, Fmoc-Glu(OtBu)-OH, Fmoc-Pro-OH, Fmoc-Leu-OH, Fmoc-Ile-OH, Fmoc-Phe-OH, Fmoc-Ser(tBu)-OH, Fmoc-Thr(tBu)-OH, Fmoc-Lys(Boc)-OH, Fmoc-Gln(Trt)-OH, Fmoc-Trp(Boc)-OH, Fmoc-Met-OH, Fmoc-Asn(Trt)-OH, Fmoc-Tyr(tBu)-OH, Fmoc-Ahx-OH, Trt-Mercaptoacetic acid.


HBTU [2-(1H-Benzotriazole-1-yl)-1,1,3,3-tetamethylaminium hexafluorophosphate]/HOBt [N-Hydroxybenzotriazole]/NMM [4-Methylmorpholine] were used as the coupling reagents. In 20% of DMF, piperidine was used to remove Fmoc. In order to remove the protection from residue or to separate the synthesized peptide from Resin, cleavage cocktail [TFA (trifluoroacetic acid)/TIS (triisopropylsilane)/EDT (ethanedithiol)/H2O=92.5/2.5/2.5/2.5] was used.
Peptides were synthesized by using the solid phase scaffold by adding each amino acid with the sequential processes as follow; amino acid protection, coupling reaction, washing, and deprotection. After cutting off the synthesized peptide from the resin, it was purified by HPLC and verify for synthesis by MS, and then freeze-dried.
Specific peptide synthesis process is described by the following with example of Pep1 of SEQ ID NO: 164 (EARPALLTSRLRFIPK).
1) Coupling
The amino acid (8 equivalent) protected with NH2-Lys(Boc)-2-chloro-Trityl Resin was melted in coupling agent HBTU (8 equiv.)/HOBt (8 equiv.)/NMM (16 equiv.), and upon addition of DMF, the reaction mixture was incubated at room temperature for 2 hours, then washed sequentially with DMF, MeOH, and DMF.
2) Fmoc Deprotection
Following the addition of 20% piperidine in DMF, the reaction mixture was incubated at room temperature for 5 minutes 2 times, then washed sequentially with DMF, MeOH, and DMF.
3) Make the Basic Framework of Peptide by Repeating Reactions 1 and 2 Repeatedly.
4) Cleavage: Add Cleavage Cocktail to the Completely Synthesized Peptide and Separate the Peptide From the Resin.
5) Add pre-chilled diethyl ether into the mixture, and then centrifuge the reaction mixture to precipitate out the peptides.
6) After purification by Prep-HPLC, Check the Molecular Weight by LC/MS and Lyophilize to Obtain the Peptides in a Powder Form.
Example 2: Preparation of Pep(CPP)-FITC Conjugate
(1) Preparation of FITC-CPP Conjugate
A conjugate of the peptides with SEQ ID NO: 1 to SEQ ID NO: 156 combined with FITC was manufactured as follows, for example, a conjugate of pep1 (SEQ ID NO: 157) and FITC, in other words, FITC-linker-pep1 was manufactured as follows.
The basic framework of peptide, NH2-linker-E(OtBu)-A-R(Pbf)-P-A-L-L-T(tBu)-S(tBu)-R(PbOL-R(Pbf)-F-I-P-K(Boc)-2-chloro-Trityl Resin) which was obtained according to the manufacturing methods described in Example 1, was reacted with FITC. Specifically, FITC (fluorescein-5isothiocyanate) (8 equivalent) and DIPEA (N,N-Diisopropylethylamine) (16 equivalent) were melted in DMF. The DMF solution was added, and reacted at room temperature for 2 hours, then washed sequentially with DMF, MeOH and DMF. As a result, FITC-linker-E(OtBu)-A-R(Pbf)-P-A-L-L-T(tBu)-S(tBu)-R(Pbf)L-R(Pbf)-F-I-P-K(Boc)-2-chloro-Trityl Resin was obtained. The linker herein is 6-aminohexanoic acid, Ahx. TFA/TIS/H2O=95/2.5/2.5 was added to the peptide made on the resin, and the conjugate was separated from the resin. A pre-chilled diethyl ether was added to the obtained mixture, and centrifugation was used to precipitate the peptide conjugates. After purification by Prep-HPLC, purity was confirmed with the analytical HPLC and the molecular weight was determined by LC/MS. The peptide synthesized as described above was verified as FITC-pep1 by confirmation of the molecular weight by LC/MS. Then the conjugates were lyophilized. SEQ ID NO: 1 to SEQ ID NO: 156 of peptides fused FITC with conjugate was also prepared as pep 1 of SEQ ID NO: 157.
(2) Preparation of a CPP-FITC Conjugate
The basic framework of the peptide, (NH2-E(OtBu)-A-R(Pbf)-P-A-L-L-T(tBu)-S(tBu)-R(Pbf)L-R(Pbf)-F-I-P-K(Dde)-2-chloro-Trityl Resin) was generated according to the manufacturing methods described in the Example 2 1. (1). To selectively introduce FITC to the C-term of the peptide, the N-term of the peptide was protected from Boc. Then, the Di-tert-butyl dicarbonate (30 equivalent) and DIPEA (30 equivalent) were melted in DMF. The DMF solution was added to the peptide and incubated at room temperature for 2 hours, and the peptide was washed sequentially with DMF, MeOH, and DMF. As a result, Boc-E(OtBu)-A-R(Pbf)-P-A-L-L-T(tBu)-S(tBu)-R(PbOL-R(Pbf)-F-I-P-K(Dde)-2-chloro-Trityl Resin was obtained. Hydrazine in 2% of DMF was used to remove Dde which is the protecting group of the C-terminal residue Lys in order to add FITC to the C-terminal of Lys. Then, FITC (8 equivalent) and DIPEA (16 equivalent) were melted in DMF which was added to the peptide reaction mixture, and the mixture was incubated at room temperature for 2 hours, then washed sequentially with DMF, MeOH, DMF. As a result, Boc-E(OtBu)-A-R(Pbf)-P-A-L-L-T(tBu)-S(tBu)-R(Pbf)L-R(Pbf)-F-I-P-K(FITC)-2-chloro-Trityl Resin was obtained. TFA/TIS/H2O=95/2.5/2.5 was added to separate the peptide from resin. Pre-chilled diethyl ether was added to the mixture, and centrifugation was used to precipitate the peptides. After purification by Prep-HPLC, purity was confirmed with the analytical HPLC and the molecular weight was confirmed with LC/MS. The obtained substances were verified as pep1-FITC by confirmation of the molecular weight by LC/MS. Then the conjugates were lyophilized. SEQ ID NO: 1 to SEQ ID NO: 156 of peptide-FITC conjugates were also prepared in the same manner as described above pep1-FITC.
Example 3: Experimental Cell Penetration of Pep(CPP)-FITC Conjugate
(1) Experimental Cell Penetration in HeLa Cell Line
Cell Culture
Homo sapiens cervix adenocarcinoma cell line as HeLa cell lines were purchased from ATCC (American Type Cell Culture). The cells were cultured in MEM supplemented with 10% fetal bovine serum (Invitrogen, USA), Earle's salts, non-essential amino acids, sodium pyruvate and 100 μg/ml penicillin and 10 units/ml streptomycin and cultured at 37° C., 5% CO2 incubator.
Flow Cytometry and Confocal Microscopy Analysis of Cell Penetrating
Flow cytometry and Confocal microscope analysis were performed to compare the between degree of cellular uptake of the cells were treated with SEQ ID NO: 1 to SEQ ID NO: 156, pep (CPP) and control.
The cell line was divided in a 6-well plate and cultured in a medium containing 10% fetal bovine serum (Invitrogen, USA), 100 μg/ml penicillin, 100 units/ml streptomycin at 37° C., 5% CO2 incubator for 12 hours. After washing the cell line with PBS, starvation was induced in a Minimum Essential Medium for an hour. 20 uM of each carrier peptide was treated and cultured at 37° C. for an hour. After repeating the step of washing the cells with PBS for three times, Trypsin-EDTA was treated form 10 mins at 37° C. to separate the carrier peptide on the outside of the cell. cells were collected with refrigerated PBS and centrifugation was performed to repeat the step of washing the cells for three times. After then, the cells were suspended in 0.5 ml of PBS containing 4% Paraformaldehyde and fluorescence of the cells was analyzed using FACS Calibur (Becton Dickinson). The cellular uptake aspect of control and various peptides combined with FITC was compared and analyzed by MFI (Mean Fluorescence Intensity).
The results are as shown in FIGS. 1 to 23. Analysis results shown in FIG. 1 to FIG. 23 are given in detail in Table 3 below.














TABLE 3








pep2
pep3
pep4
pep5
pep6
PelP7
pep8





control
3.98 ± 0.87
2.39 ± 0.23
3.98 ± 0.87
3.98 ± 0.87
3.98 ± 0.87
3.98 ± 0.87
3.98 ± 0.87


peptide
37.6 ± 1.89
18.23 ± 3.21 
28.35 ± 2.54 
19.06 ± 1.33 
15.81 ± 1.59 
9.51 ± 2.95
17.75 ± 1.96 






pep11
pep12
pep13
pep14
pep15
pep6
pep16





control
3.98 ± 0.87
2.39 ± 0.23
3.98 ± 0.87
3.98 ± 0.87
2.39 ± 0.23
3.98 ± 0.87
3.98 ± 0.87


peptide
559.78 ± 3.41 
10.46 ± 1.99 
 22 ± 2.6
14.01 ± 1.32 
10.51 ± 3.71 
35.61 ± 1.29 
20.12 ± 1.84 






pep17
pep18
pep19
pep20
pep21
pep22
pep23





control
3.98 ± 0.87
3.98 ± 0.87
2.39 ± 0.23
3.98 ± 0.87
2.39 ± 0.23
3.98 ± 0.87
3.98 ± 0.87


peptide
15.26 ± 2.23 
18.7 ± 1.21
13.04 ± 3.41 
20.73 ± 2.45 
13.22 ± 1.67 
13.56 ± 0.23 
15.68 ± 2.03 






pep24
pep25
pep26
pep27
pep28
pep29
pep30





control
3.98 ± 0.87
3.98 ± 0.87
3.98 ± 0.87
3.98 ± 0.87
3.98 ± 0.87
3.98 ± 0.87
3.98 ± 0.87


peptide
13.78 ± 1.07 
18.62 ± 0.69 
14.81 ± 1.77 
9.89 ± 2.51
14.08 ± 3.11 
33.5 ± 2.48
14.37 ± 1.73 






pep31
pep32
pep38
pep34
pep35
pep36
pep37





control
3.98 ± 0.87
3.98 ± 0.87
3.98 ± 0.87
3.98 ± 0.87
3.98 ± 0.87
3.98 ± 0.87
3.98 ± 0.87


peptide
14.93 ± 2.05 
11.98 ± 2.92 
14.36 ± 1.63 
14.41 ± 3.44 
15.54 ± 1.38 
15.37 ± 2.65 
20.81 ± 1.02 






pep38
pep39
pep40
pep41
pep42
pep43
pep45





control
3.31 ± 0.62
3.98 ± 0.87
3.98 ± 0.87
3.98 ± 0.87
3.98 ± 0.87
3.98 ± 0.87
3.31 ± 0.62


peptide
380.4 ± 2.6 
21.4 ± 0.99
11.63 ± 1.11 
28.97 ± 3.44 
20.82 ± 1.93 
29.88 ± 2.35 
203.77 ± 3.26 






pep46
pep47
pep48
pep49
pep50
pep51
pep52





control
3.98 ± 0.87
3.98 ± 0.87
3.98 ± 0.87
3.98 ± 0.87
2.39 ± 0.23
3.98 ± 0.87
3.98 ± 0.87


peptide
24.67 ± 2.02 
15.85 ± 1.03 
26.51 ± 2.36 
13.91 ± 0.51 
2228.76 ± 3.68  
59.81 ± 1.38 
16.67 ± 2.22 






pep53
pep54
pep56
pep57
pep58
pep59
pep60





control
3.98 ± 0.87
2.52 ± 0.41
3.98 ± 0.87
2.39 ± 0.23
3.98 ± 0.87
3.98 ± 0.87
3.98 ± 0.87


peptide
16.16 ± 0.66 
388.45 ± 2.94 
28.61 ± 0.44 
568.02 ± 3.01 
19.87 ± 0.94 
18.58 ± 0.52 
15.79 ± 1.63 






pep62
pep63
pep65
pep66
pep68
pep70
pep71





control
3.98 ± 0.87
3.98 ± 0.87
3.98 ± 0.87
2.52 ± 0.41
3.98 ± 0.87
3.98 ± 0.87
3.98 ± 0.87


peptide
14.55 ± 3.71 
21.3 ± 1.55
12.72 ± 1.36 
20.7 ± 2.57
27.28 ± 1.28 
21.82 ± 1.76 
16.61 ± 0.88 






pep72
pep76
pep79
pep80
pep81
pep82
pep83





control
3.98 ± 0.87
2.52 ± 0.41
3.98 ± 0.87
3.98 ± 0.87
2.39 ± 0.23
3.98 ± 0.87
3.98 ± 0.87


peptide
17.57 ± 2.31 
13.98 ± 0.11 
31.2 ± 1.52
12.45 ± 0.46 
29.69 ± 2.49 
22.44 ± 3.32 
24.57 ± 1.36 






pep87
pep88
pep89
pep91
pep92
pep94
pep95





control
2.52 ± 0.41
3.98 ± 0.87
3.98 ± 0.87
3.98 ± 0.87
3.98 ± 0.87
2.39 ± 0.23
3.98 ± 0.87


peptide
38.35 ± 2.27 
16.43 ± 0.55 
13.54 ± 0.75 
24.41 ± 2.79 
19.91 ± 0.51 
117.76 ± 4.24 
10.92 ± 0.07 






pep96
pep102
pep103
pep104
pep105
pep106
pep107





control
2.39 ± 0.23
2.52 ± 0.41
3.98 ± 0.87
2.39 ± 0.23
3.98 ± 0.87
3.98 ± 0.87
3.98 ± 0.87


peptide
308.53 ± 3.61 
45.84 ± 1.58 
126.88 ± 2.14 
36.74 ± 1.11 
13.23 ± 2.21 
12.45 ± 0.38 
23.11 ± 0.56 






pep108
pep109
pep110
pep113
pep116
pep117
pep118





control 
3.98 ± 0.87
3.98 ± 0.87
3.98 ± 0.87
3.98 ± 0.87
3.98 ± 0.87
3.98 ± 0.87
2.53 ± 0.17


peptide 
22.53 ± 0.95 
12.46 ± 1.32 
22.04 ± 0.77 
16.09 ± 2.5 
20.5 ± 0.97
23.11 ± 2.46 
14.69 ± 0.33 






pep119
pep123
pep124
pep125
pep126
pep128
pep131





control
2.53 ± 0.17
3.98 ± 0.87
2.53 ± 0.17
2.53 ± 0.17
2.53 ± 0.17
3.98 ± 0.87
2.39 ± 0.23


peptide
13.23 ± 0.64 
18.03 ± 2.83 
23.74 ± 3.62 
28.62 ± 2.12 
3362.29 ± 3.24  
14.36 ± 1.21 
32.51 ± 2.38 






pep132
pep133
pep134
pep138
pep139
pep141
pep145





control
3.98 ± 0.87
2.53 ± 0.17
2.39 ± 0.23
3.98 ± 0.87
2.39 ± 0.23
3.98 ± 0.87
3.98 ± 0.87


peptide 
26.39 ± 0.95 
46.79 ± 1.92 
78.05 ± 2.26 
16.43 ± 0.91 
29.95 ± 0.28 
20.89 ± 0.31 
11.97 ± 0.78 






pep146
pep150
pep151
pep153
pep158
pep160





control
2.53 ± 0.17
3.98 ± 0.87
3.98 ± 0.87
2.53 ± 0.17
3.98 ± 0.87
2.53 ± 0.17



peptide
18.32 ± 1.82 
17.46 ± 0.24 
23.52 ± 0.56 
31.72 ± 1.74 
87.75 ± 2.51 
9.860.23









(2) Cell Penetrating Property in Huh7 Cell Line
Cell Culture
human hepatocellular carcinoma cell line as Huh7 lines were purchased from ATCC
(American Type Cell Culture) and used as suspended cells. The cells were cultured in MEM supplemented with 10% fetal bovine serum (Invitrogen, USA), EarleSA), Earle Huh7 lines were purchased from ATpyruvate and 100 μa/ml penicillin and 10 units/ml streptomycin and cultured at 37° C., 5% CO2 incubator.
The Screening Analysis of Cell Penetrating Used by Flow Cytometry
To confirm the cell penetrating of peptides, the Huh7 cell lines were treated with SEQ ID NO: 1 to SEQ ID NO: 156 and analysed by Flow cytometry. The analysed method was also confirmed by the same manner as described above example (1) in Hela cell lines. Also, the result showed in FIG. 24 to FIG. 43.
(3) Experimental Cell Penetration in Human T Lymphocyte Cell Lines
Cell Culture
human T-cell leukemia cell line as Jurket was purchased from ATCC (American Type Cell Culture) and used as suspended cells. The cells were cultured in RPMI 1640 supplemented with 10% fetal bovine serum (Invitrogen, USA), EarleSA), EarleC (American Type Cell Culture) and pyruvate and 100 om ATCC (American Type units/ml streptomycin and cultured at 37° C., 5% CO2 incubator. Human derived lymphocytes (lymphocyte) separated from the healthy human blood (50 m) and then collected layer of peripheral blood mononuclear cells (PBMC) and lymphocytes used by Biocoll separating solution (Biochrom AG, Berlin, Germany).
The Screening Analysis of Cell Penetrating Used by Flow Cytometry
To confirm the cell penetrating of peptides, the human T-cell leukemia cell lines were treated with SEQ ID NO: 1 to SEQ ID NO: 156 and analysed by Flow cytometry. The analysed method was also confirmed by the same manner as described above example (1) in Hela cell lines. Also, the result showed in FIG. 44 to FIG. 58.
(4) Analysis of Cell Viability and Toxicity
The cell line was divided into 96-well plate and cultured in a medium containing 10% fetal bovine serum (Invitrogen, USA), 100 μg/ml penicillin, 100 units/ml streptomycin at 37° C., 5% CO2 incubator for 12 hours. After washing the cell line with PBS, starvation was induced in a Minimum Essential Medium for an hour. 20 uM of each carrier peptide was treated and cultured at 37° C. for an hour. After cultured cells, analysed cell viability and toxicity used by MTT assay. The results showed in FIG. 59 to FIG. 72.

## Claims

1. A conjugate consisting of a mitochondrial targeting peptide and ferrocene carboxylate, wherein said mitochondrial targeting peptide consists of 30 amino acids or less.

2. The conjugate of claim 1, wherein the mitochondrial targeting peptide is derived from human telomerase reverse transcriptase (hTERT) enzyme.

3. The conjugate of claim 2, wherein the mitochondrial targeting peptide comprises SEQ ID NO: 48.

4. The conjugate of claim 1, wherein the mitochondrial targeting peptide and ferrocene carboxylate are combined via a covalent bond.

5. The conjugate of claim 1, wherein the mitochondrial targeting peptide and ferrocene carboxylate are combined via a non-covalent bond.

6. A contrasting agent comprising the conjugate of claim 1.

7. A composition comprising the conjugate of claim 1 and a pharmaceutically acceptable carrier.