# Patent

## Title
Anti-blood dendritic cell antigen 2 antibodies and uses thereof

## Abstract

Antibodies and antibody fragments that bind to BDCA2 are disclosed. Also disclosed are methods of using the antibodies and antibody fragments to induce death of a plasmacytoid dendritic cell, inhibit production or secretion of inflammatory cytokines and chemokines, and treat or prevent immunological disorders such as inflammatory and autoimmune conditions.

## Background
CROSS REFERENCE TO RELATED APPLICATIONS
This application claims the benefit of U.S. Provisional Application No. 61/735,362 filed Dec. 10, 2012 and U.S. Provisional Application No. 61/763,270 filed Feb. 11, 2013, the contents of both of which are incorporated herein by reference in their entireties.


BACKGROUND
Blood dendritic cell antigen 2 (BDCA2) is a C-type lectin expressed on human plasmacytoid dendritic cells (pDCs) (Dzionek et al., J. Immunol., 165:6037-6046 (2000)), a specialized population of bone marrow-derived cells that secrete type I interferons (IFNs) in response to toll-like receptor (TLR) ligands. BDCA2 consists of a single extracellular carbohydrate recognition domain (CRD), which belongs to the type II C-type lectin group, at its C-terminus, a transmembrane region, and a short cytoplasmic tail at its N-terminus that does not harbor a signaling motif. BDCA2 transmits intracellular signals through an associated transmembrane adaptor, the FcεRIγ, and induces a B cell receptor (BCR)-like signaling cascade.
SUMMARY
This disclosure is based, at least in part, on the identification and characterization of antibodies that bind to BDCA2. Such antibodies can reduce or inhibit the secretion of inflammatory cytokines and chemokines. The anti-BDCA2 antibodies described herein are also capable of depleting pDCs by antibody dependent cellular cytotoxicity (ADCC) or complement-mediated cytotoxicity (CDC). In addition, anti-BDCA2 antibodies described herein can downregulate levels of CD32a and/or CD62L on the surface of pDCs. Furthermore, the anti-BDCA2 antibodies of this disclosure can mediate internalization of BDCA2 from the cell surface of pDCs. For at least these reasons, the anti-BDCA2 antibodies described herein are useful in treating or preventing autoimmune and inflammatory conditions. This disclosure also shows that anti-BDCA2 antibodies described herein can be combined with an antimalarial agent for improved effects.
In one aspect, the disclosure features an isolated antibody or antigen-binding fragment thereof that selectively binds to the ectodomain of human BDCA2 (SEQ ID NO:1) and competes with BIIB059 for binding to the extracellular domain of human BDCA2.
An anti-BDCA2 antibody or antigen-binding fragment thereof competes with BIIB059 for binding to BDCA2 when the anti-BDCA2 antibody or antigen-binding fragment thereof's prior binding to BDCA2 completely or partially inhibits later binding of BIIB059 to BDCA2. For example, an anti-BDCA2 antibody or antigen-binding fragment thereof competes with BIIB059 for binding to BDCA2 when the anti-BDCA2 antibody or antigen-binding fragment thereof's prior binding to BDCA2 completely inhibits later binding of BIIB059 to BDCA2. In certain embodiments, the anti-BDCA2 antibody or antigen-binding fragment thereof's prior binding to BDCA2 results in at least 30%, 50%, 70%, 80%, 90%, 95%, 98% or 99% inhibition of later binding of BIIB059 to BDCA2.
In another aspect, the disclosure features an isolated antibody or antigen-binding fragment thereof selectively binds to the ectodomain of human BDCA2 (SEQ ID NO:1) and: (i) inhibits secretion of type I interferons and/or type III interferons in addition to other cytokines and chemokines from plasmacytoid dendritic cells; or (ii) induces or enhances depletion of plasmacytoid dendritic cells in vitro. In certain embodiments, the anti-BDCA2 antibody downregulates CD32a and/or CD62L from the surface of pDCs. In some embodiments, the anti-BDCA2 antibody mediates internalization of BDCA2 from the cell surface of pDCs. In some embodiments, the antibody or antigen-binding fragment thereof binds to cynomolgus BDCA2 (SEQ ID NO:72) and rhesus BDCA2 (SEQ ID NO:72). In certain embodiments, the isolated antibody or antigen-binding fragment thereof inhibits secretion or production of type I interferon, interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), type III interferon, macrophage inflammatory protein-1 (MIP-1)-α/CCL3, MIP-1β/CCL4, chemokine (C—C motif) ligand 5 (CCL5/RANTES), or interferon γ-induced protein-10 (IP-10/CXCL10).
In some embodiments of the above two aspects, the isolated antibody or antigen-binding fragment thereof optionally further comprises or consists of one, two, three, four, five, or six, of the following features: an EC50 (human BDCA2) of 0.5 to 3 μg/mL or 4 nM to 10 nM; an EC50 (cynomolgus BDCA2) of 0.5 to 3 μg/mL or 5 nM to 10 nM; a pI of 7 to 7.5; does not bind rat Clec4b2, or binds rat Clec4b2 with a lower binding affinity than human, cynomolgus or rhesus BDCA2; inhibits production or secretion of chemokines such as MIP-1-α/CCL3, MIP-1β/CCL4, CCL5/RANTES, IP-10/CXCL10; a heavy chain CDR1, a heavy chain CDR2, and a heavy chain CDR3, wherein the heavy chain CDR1 has an amino acid sequence consisting of the amino acid sequence set forth in SEQ ID NO:9 or an amino acid sequence consisting of the amino acid sequence set forth in SEQ ID NO:8; the heavy chain CDR2 has an amino acid sequence consisting of the amino acid sequence set forth in SEQ ID NO:10; and the heavy chain CDR3 has an amino acid sequence consisting of the amino acid sequence set forth in SEQ ID NO:11; and a variable heavy chain comprising or consisting of the amino acid sequence set forth in SEQ ID NO:24. In certain embodiments, the antibody or antigen-binding fragment thereof has a heavy chain CDR1 consisting of the amino acid sequence set forth in SEQ ID NO:89; a heavy chain CDR2 consisting of the amino acid sequence set forth in SEQ ID NO:91; and a heavy chain CDR3 consisting of the amino acid sequence set forth in SEQ ID NO:11. In certain embodiments, the antibody or antigen-binding fragment thereof has a heavy chain CDR1 consisting of the amino acid sequence set forth in SEQ ID NO:9; a heavy chain CDR2 consisting of the amino acid sequence set forth in SEQ ID NO:92; and a heavy chain CDR3 consisting of the amino acid sequence set forth in SEQ ID NO:11. In certain embodiments, the antibody or antigen-binding fragment thereof has a heavy chain CDR1 consisting of the amino acid sequence set forth in SEQ ID NO:90; a heavy chain CDR2 consisting of the amino acid sequence set forth in SEQ ID NO:93; and a heavy chain CDR3 consisting of the amino acid sequence set forth in SEQ ID NO:94. In some embodiments, the isolated antibody or antigen-binding fragment has an EC50 (human BDCA2) of 4.5 nM, 4.6 nM, 4.7 nM, 4.8 nM, 4.9 nM, 5.0 nM, 5.1 nM, 5.2 nM, 5.3 nM, 5.4 nM, or 5.5 nM. In a specific embodiment, the isolated antibody or antigen-binding fragment has an EC50 (human BDCA2) of 4.9 nM. In some embodiments, the isolated antibody or antigen-binding fragment has an EC50 (cynomolgus BDCA2) of 4.0 nM, 4.1 nM, 4.2 nM, 4.3 nM, 4.4 nM, 4.5 nM, 4.6 nM, 4.7 nM, 4.8 nM, 4.9 nM, or 5.0 nM. In a specific embodiment, the isolated antibody or antigen-binding fragment has an EC50 (cynomolgus BDCA2) of 4.4 nM. In certain embodiments of this aspect, the antibody has a human heavy chain and light chain constant region. In certain embodiments, the heavy chain constant region comprises a CH1 domain and a hinge region. In some embodiments, the heavy chain constant region comprises a CH3 domain. If the heavy chain constant region includes substitutions, such substitutions modify the properties of the antibody (e.g., increase or decrease one or more of: Fc receptor binding, antibody glycosylation, the number of cysteine residues, effector cell function, or complement function). In certain embodiments, the antibody is an IgG antibody. In specific embodiments, the antibody is selected from the group consisting of IgG1, IgG2, IgG3, and IgG4. In certain embodiments, the antibody includes a human Fc region that binds FcγRIIa (CD32a) with an EC50 of 7 to 15 μg/mL. In certain embodiments, the antibody includes a human Fc region that binds FcγRIIa (CD32a) with an EC50 of 10 μg/mL. In certain embodiments, the antibody includes a human Fc region that binds FcγRIIa (CD32a) with an EC50 of 11 μg/mL. In certain embodiments, the antibody includes a human Fc region that binds FcγRIIa (CD32a) with an EC50 of 12 μg/mL.
In another aspect, the disclosure features an isolated antibody or antigen-binding fragment thereof that selectively binds to the ectodomain of human BDCA2 (SEQ ID NO:1), and comprises a heavy chain CDR1, a heavy chain CDR2, and a heavy chain CDR3. The heavy chain CDR1 comprises or consists of the amino acid sequence GFTFSTYTMS (SEQ ID NO:9) or the amino acid sequence set forth in SEQ ID NO:9 with a substitution at one, two, three, or four amino acid positions. The heavy chain CDR2 comprises or consists of the amino acid sequence TISPGDSFGYYYPDSVQG (SEQ ID NO:10) or the amino acid sequence set forth in SEQ ID NO:10 with a substitution at one, two, three, or four amino acid positions. The heavy chain CDR3 comprises or consists of the amino acid sequence DIYYNYGAWFAY (SEQ ID NO:11) or the amino acid sequence set forth in SEQ ID NO:11 with a substitution at one, two, three, or four amino acid positions. In another aspect, the antibody or antigen-binding fragment thereof comprises a heavy chain CDR1 consisting of the amino acid sequence set forth in SEQ ID NO:89 with a substitution at one, two, three, or four amino acid positions; a heavy chain CDR2 consisting of the amino acid sequence set forth in SEQ ID NO:91 with a substitution at one, two, three, or four amino acid positions; and a heavy chain CDR3 consisting of the amino acid sequence set forth in SEQ ID NO:11 with a substitution at one, two, three, or four amino acid positions. In another aspect, the antibody or antigen-binding fragment thereof comprises a heavy chain CDR1 consisting of the amino acid sequence set forth in SEQ ID NO:9 with a substitution at one, two, three, or four amino acid positions; a heavy chain CDR2 consisting of the amino acid sequence set forth in SEQ ID NO:92 with a substitution at one, two, three, or four amino acid positions; and a heavy chain CDR3 consisting of the amino acid sequence set forth in SEQ ID NO:11 with a substitution at one, two, three, or four amino acid positions. In another aspect, the antibody or antigen-binding fragment thereof comprises a heavy chain CDR1 consisting of the amino acid sequence set forth in SEQ ID NO:90 with a substitution at one, two, three, or four amino acid positions; a heavy chain CDR2 consisting of the amino acid sequence set forth in SEQ ID NO:93 with a substitution at one, two, three, or four amino acid positions; and a heavy chain CDR3 consisting of the amino acid sequence set forth in SEQ ID NO:94 with a substitution at one, two, three, or four amino acid positions. These antibodies (i) bind human or cynomolgus monkey BDCA2 but do not significantly bind BDCA2 from phylogenetic species below primates; and/or (ii) inhibit TLR7/TLR9-induced type I interferon and other cytokine or chemokine production by human pDCs; and/or (iii) mediate internalization of BDCA2 from the surface of pDCs; and/or (iv) downregulate CD32a and/or CD62L from the surface of pDCs; and/or (v) deplete pDCs in vitro by ADCC or CDC. In certain embodiments of this aspect, the antibody has a human heavy chain and light chain constant region.
In certain embodiments, the isolated antibody or antigen-binding fragment thereof that specifically binds human BDCA2 has a heavy chain CDR1 that comprises or consists of the amino acid sequence GFTFSTYTMS (SEQ ID NO:9) or the amino acid sequence set forth in SEQ ID NO:9 with a substitution at one or two amino acid positions; a heavy chain CDR2 that comprises or consists of the amino acid sequence TISPGDSFGYYYPDSVQG (SEQ ID NO:10) or the amino acid sequence set forth in SEQ ID NO:10 with a substitution at one or two amino acid positions; and a heavy chain CDR3 that comprises or consists of the amino acid sequence DIYYNYGAWFAY (SEQ ID NO:11) or the amino acid sequence set forth in SEQ ID NO:11 with a substitution at one or two amino acid positions. In other embodiments of this aspect, the isolated antibody or antigen-binding fragment has a heavy chain CDR1 that comprises or consists of the amino acid sequence GFTFSTYTMS (SEQ ID NO:9); a heavy chain CDR2 comprises or consists of the amino acid sequence TISPGDSFGYYYPDSVQG (SEQ ID NO:10); and a heavy chain CDR3 comprises or consists of the amino acid sequence DIYYNYGAWFAY (SEQ ID NO:11). In other embodiments of this aspect, the isolated antibody or antigen-binding fragment comprises a light chain CDR1, a light chain CDR2, and a light chain CDR3. The light chain CDR1 comprises or consists of the amino acid sequence KASQSVDYDGDSYMN (SEQ ID NO:5) or the amino acid sequence set forth in SEQ ID NO:5 with a substitution at one, two, three, or four amino acid positions. The light chain CDR2 comprises or consists of the amino acid sequence AASTLES (SEQ ID NO:6) or the amino acid sequence set forth in SEQ ID NO:6 with a substitution at one, two, three, or four amino acid positions. The light chain CDR3 comprises or consists of the amino acid sequence QQANEDPRT (SEQ ID NO:7) or the amino acid sequence set forth in SEQ ID NO:7 with a substitution at one, two, three, or four amino acid positions. In certain embodiments, the light chain CDR1 comprises or consists of the amino acid sequence KASQSVDYDGDSYMN (SEQ ID NO:5) or the amino acid sequence set forth in SEQ ID NO:5 with a substitution at one or two amino acid positions; the light chain CDR2 comprises or consists of the amino acid sequence AASTLES (SEQ ID NO:6) or the amino acid sequence set forth in SEQ ID NO:6 with a substitution at one or two amino acid positions; and the light chain CDR3 comprises or consists of the amino acid sequence QQANEDPRT (SEQ ID NO:7) or the amino acid sequence set forth in SEQ ID NO:7 with a substitution at one or two amino acid positions. In other embodiments, the isolated antibody or antigen-binding fragment thereof has a heavy chain CDR1 that comprises or consists of the amino acid sequence GFTFSTYTMS (SEQ ID NO:9); a heavy chain CDR2 that comprises or consists of the amino acid sequence TISPGDSFGYYYPDSVQG (SEQ ID NO:10); a heavy chain CDR3 that comprises or consists of the amino acid sequence DIYYNYGAWFAY (SEQ ID NO:11); a light chain CDR1 that comprises or consists of the amino acid sequence KASQSVDYDGDSYMN (SEQ ID NO:5); a light chain CDR2 comprises or consists of the amino acid sequence AASTLES (SEQ ID NO:6); and a light chain CDR3 that comprises or consists of the amino acid sequence QQANEDPRT (SEQ ID NO:7).
In certain embodiments, the isolated antibody or antigen-binding fragment thereof that selectively binds human BDCA2 comprises a heavy chain CDR1 that comprises or consists of the amino acid sequence TYTMS (SEQ ID NO:8) or the amino acid sequence set forth in SEQ ID NO:8 with a substitution at one or two amino acid positions; a heavy chain CDR2 that comprises or consists of the amino acid sequence TISPGDSFGYYYPDSVQG (SEQ ID NO:10) or the amino acid sequence set forth in SEQ ID NO:10 with a substitution at one or two amino acid positions; and a heavy chain CDR3 that comprises or consists of the amino acid sequence DIYYNYGAWFAY (SEQ ID NO:11) or the amino acid sequence set forth in SEQ ID NO:11 with a substitution at one or two amino acid positions. In other embodiments of this aspect, the isolated antibody or antigen-binding fragment has a heavy chain CDR1 that comprises or consists of the amino acid sequence TYTMS (SEQ ID NO:8); a heavy chain CDR2 comprises or consists of the amino acid sequence TISPGDSFGYYYPDSVQG (SEQ ID NO:10); and a heavy chain CDR3 comprises or consists of the amino acid sequence DIYYNYGAWFAY (SEQ ID NO:11). In other embodiments of this aspect, the isolated antibody or antigen-binding fragment comprises a light chain CDR1, a light chain CDR2, and a light chain CDR3. The light chain CDR1 comprises or consists of the amino acid sequence KASQSVDYDGDSYMN (SEQ ID NO:5) or the amino acid sequence set forth in SEQ ID NO:5 with a substitution at one, two, three, or four amino acid positions. The light chain CDR2 comprises or consists of the amino acid sequence AASTLES (SEQ ID NO:6) or the amino acid sequence set forth in SEQ ID NO:6 with a substitution at one, two, three, or four amino acid positions. The light chain CDR3 comprises or consists of the amino acid sequence QQANEDPRT (SEQ ID NO:7) or the amino acid sequence set forth in SEQ ID NO:7 with a substitution at one, two, three, or four amino acid positions. In certain embodiments, the light chain CDR1 comprises or consists of the amino acid sequence KASQSVDYDGDSYMN (SEQ ID NO:5) or the amino acid sequence set forth in SEQ ID NO:5 with a substitution at one or two amino acid positions; the light chain CDR2 comprises or consists of the amino acid sequence AASTLES (SEQ ID NO:6) or the amino acid sequence set forth in SEQ ID NO:6 with a substitution at one or two amino acid positions; and the light chain CDR3 comprises or consists of the amino acid sequence QQANEDPRT (SEQ ID NO:7) or the amino acid sequence set forth in SEQ ID NO:7 with a substitution at one or two amino acid positions. In other embodiments, the isolated antibody or antigen-binding fragment thereof has a heavy chain CDR1 that comprises or consists of the amino acid sequence TYTMS (SEQ ID NO:8); a heavy chain CDR2 that comprises or consists of the amino acid sequence TISPGDSFGYYYPDSVQG (SEQ ID NO:10); a heavy chain CDR3 that comprises or consists of the amino acid sequence DIYYNYGAWFAY (SEQ ID NO:11); a light chain CDR1 that comprises or consists of the amino acid sequence KASQSVDYDGDSYMN (SEQ ID NO:5); a light chain CDR2 comprises or consists of the amino acid sequence AASTLES (SEQ ID NO:6); and a light chain CDR3 that comprises or consists of the amino acid sequence QQANEDPRT (SEQ ID NO:7).
In certain embodiments, the isolated antibody or antigen-binding fragment thereof that selectively binds human BDCA2 comprises a heavy chain CDR1 that comprises or consists of the amino acid sequence GFTFSTY (SEQ ID NO:89) or the amino acid sequence set forth in SEQ ID NO:89 with a substitution at one or two amino acid positions; a heavy chain CDR2 that comprises or consists of the amino acid sequence SPGDSFG (SEQ ID NO:91) or the amino acid sequence set forth in SEQ ID NO:91 with a substitution at one or two amino acid positions; and a heavy chain CDR3 that comprises or consists of the amino acid sequence DIYYNYGAWFAY (SEQ ID NO:11) or the amino acid sequence set forth in SEQ ID NO:11 with a substitution at one or two amino acid positions. In other embodiments of this aspect, the isolated antibody or antigen-binding fragment has a heavy chain CDR1 that comprises or consists of the amino acid sequence GFTFSTY (SEQ ID NO:89); a heavy chain CDR2 comprises or consists of the amino acid sequence SPGDSFG (SEQ ID NO:91); and a heavy chain CDR3 comprises or consists of the amino acid sequence DIYYNYGAWFAY (SEQ ID NO:11). In other embodiments of this aspect, the isolated antibody or antigen-binding fragment comprises a light chain CDR1, a light chain CDR2, and a light chain CDR3. The light chain CDR1 comprises or consists of the amino acid sequence KASQSVDYDGDSYMN (SEQ ID NO:5) or the amino acid sequence set forth in SEQ ID NO:5 with a substitution at one, two, three, or four amino acid positions. The light chain CDR2 comprises or consists of the amino acid sequence AASTLES (SEQ ID NO:6) or the amino acid sequence set forth in SEQ ID NO:6 with a substitution at one, two, three, or four amino acid positions. The light chain CDR3 comprises or consists of the amino acid sequence QQANEDPRT (SEQ ID NO:7) or the amino acid sequence set forth in SEQ ID NO:7 with a substitution at one, two, three, or four amino acid positions. In certain embodiments, the light chain CDR1 comprises or consists of the amino acid sequence KASQSVDYDGDSYMN (SEQ ID NO:5) or the amino acid sequence set forth in SEQ ID NO:5 with a substitution at one or two amino acid positions; the light chain CDR2 comprises or consists of the amino acid sequence AASTLES (SEQ ID NO:6) or the amino acid sequence set forth in SEQ ID NO:6 with a substitution at one or two amino acid positions; and the light chain CDR3 comprises or consists of the amino acid sequence QQANEDPRT (SEQ ID NO:7) or the amino acid sequence set forth in SEQ ID NO:7 with a substitution at one or two amino acid positions. In other embodiments, the isolated antibody or antigen-binding fragment thereof has a heavy chain CDR1 that comprises or consists of the amino acid sequence GFTFSTY (SEQ ID NO:89); a heavy chain CDR2 that comprises or consists of the amino acid sequence SPGDSFG (SEQ ID NO:91); a heavy chain CDR3 that comprises or consists of the amino acid sequence DIYYNYGAWFAY (SEQ ID NO:11); a light chain CDR1 that comprises or consists of the amino acid sequence KASQSVDYDGDSYMN (SEQ ID NO:5); a light chain CDR2 comprises or consists of the amino acid sequence AASTLES (SEQ ID NO:6); and a light chain CDR3 that comprises or consists of the amino acid sequence QQANEDPRT (SEQ ID NO:7).
In certain embodiments, the isolated antibody or antigen-binding fragment thereof that selectively binds human BDCA2 comprises a heavy chain CDR1 that comprises or consists of the amino acid sequence GFTFSTYTMS (SEQ ID NO:9) or the amino acid sequence set forth in SEQ ID NO:9 with a substitution at one or two amino acid positions; a heavy chain CDR2 that comprises or consists of the amino acid sequence TISPGDSFGYY (SEQ ID NO:92) or the amino acid sequence set forth in SEQ ID NO:92 with a substitution at one or two amino acid positions; and a heavy chain CDR3 that comprises or consists of the amino acid sequence DIYYNYGAWFAY (SEQ ID NO:11) or the amino acid sequence set forth in SEQ ID NO:11 with a substitution at one or two amino acid positions. In other embodiments of this aspect, the isolated antibody or antigen-binding fragment has a heavy chain CDR1 that comprises or consists of the amino acid sequence GFTFSTYTMS (SEQ ID NO:9); a heavy chain CDR2 comprises or consists of the amino acid sequence TISPGDSFGYY (SEQ ID NO:92); and a heavy chain CDR3 comprises or consists of the amino acid sequence DIYYNYGAWFAY (SEQ ID NO:11). In other embodiments of this aspect, the isolated antibody or antigen-binding fragment comprises a light chain CDR1, a light chain CDR2, and a light chain CDR3. The light chain CDR1 comprises or consists of the amino acid sequence KASQSVDYDGDSYMN (SEQ ID NO:5) or the amino acid sequence set forth in SEQ ID NO:5 with a substitution at one, two, three, or four amino acid positions. The light chain CDR2 comprises or consists of the amino acid sequence AASTLES (SEQ ID NO:6) or the amino acid sequence set forth in SEQ ID NO:6 with a substitution at one, two, three, or four amino acid positions. The light chain CDR3 comprises or consists of the amino acid sequence QQANEDPRT (SEQ ID NO:7) or the amino acid sequence set forth in SEQ ID NO:7 with a substitution at one, two, three, or four amino acid positions. In certain embodiments, the light chain CDR1 comprises or consists of the amino acid sequence KASQSVDYDGDSYMN (SEQ ID NO:5) or the amino acid sequence set forth in SEQ ID NO:5 with a substitution at one or two amino acid positions; the light chain CDR2 comprises or consists of the amino acid sequence AASTLES (SEQ ID NO:6) or the amino acid sequence set forth in SEQ ID NO:6 with a substitution at one or two amino acid positions; and the light chain CDR3 comprises or consists of the amino acid sequence QQANEDPRT (SEQ ID NO:7) or the amino acid sequence set forth in SEQ ID NO:7 with a substitution at one or two amino acid positions. In other embodiments, the isolated antibody or antigen-binding fragment thereof has a heavy chain CDR1 that comprises or consists of the amino acid sequence GFTFSTYTMS (SEQ ID NO:9); a heavy chain CDR2 that comprises or consists of the amino acid sequence TISPGDSFGYY (SEQ ID NO:92); a heavy chain CDR3 that comprises or consists of the amino acid sequence DIYYNYGAWFAY (SEQ ID NO:11); a light chain CDR1 that comprises or consists of the amino acid sequence KASQSVDYDGDSYMN (SEQ ID NO:5); a light chain CDR2 comprises or consists of the amino acid sequence AASTLES (SEQ ID NO:6); and a light chain CDR3 that comprises or consists of the amino acid sequence QQANEDPRT (SEQ ID NO:7).
In certain embodiments, the isolated antibody or antigen-binding fragment thereof that selectively binds human BDCA2 comprises a heavy chain CDR1 that comprises or consists of the amino acid sequence STYTMS (SEQ ID NO:90) or the amino acid sequence set forth in SEQ ID NO:90 with a substitution at one or two amino acid positions; a heavy chain CDR2 that comprises or consists of the amino acid sequence WVATISPGDSFGYY (SEQ ID NO:93) or the amino acid sequence set forth in SEQ ID NO:93 with a substitution at one or two amino acid positions; and a heavy chain CDR3 that comprises or consists of the amino acid sequence TRDIYYNYGAWFA (SEQ ID NO:94) or the amino acid sequence set forth in SEQ ID NO:94 with a substitution at one or two amino acid positions. In other embodiments of this aspect, the isolated antibody or antigen-binding fragment has a heavy chain CDR1 that comprises or consists of the amino acid sequence STYTMS (SEQ ID NO:90); a heavy chain CDR2 comprises or consists of the amino acid sequence WVATISPGDSFGYY (SEQ ID NO:93); and a heavy chain CDR3 comprises or consists of the amino acid sequence TRDIYYNYGAWFA (SEQ ID NO:94). In other embodiments of this aspect, the isolated antibody or antigen-binding fragment comprises a light chain CDR1, a light chain CDR2, and a light chain CDR3. The light chain CDR1 comprises or consists of the amino acid sequence DYDGDSYMNWY (SEQ ID NO:95) or the amino acid sequence set forth in SEQ ID NO:95 with a substitution at one, two, three, or four amino acid positions. The light chain CDR2 comprises or consists of the amino acid sequence LLIYAASTLE (SEQ ID NO:96) or the amino acid sequence set forth in SEQ ID NO:96 with a substitution at one, two, three, or four amino acid positions. The light chain CDR3 comprises or consists of the amino acid sequence QQANEDPR (SEQ ID NO:97) or the amino acid sequence set forth in SEQ ID NO:97 with a substitution at one, two, three, or four amino acid positions. In certain embodiments, the light chain CDR1 comprises or consists of the amino acid sequence DYDGDSYMNWY (SEQ ID NO:95) or the amino acid sequence set forth in SEQ ID NO:95 with a substitution at one or two amino acid positions; the light chain CDR2 comprises or consists of the amino acid sequence LLIYAASTLE (SEQ ID NO:96) or the amino acid sequence set forth in SEQ ID NO:96 with a substitution at one or two amino acid positions; and the light chain CDR3 comprises or consists of the amino acid sequence QQANEDPR (SEQ ID NO:97) or the amino acid sequence set forth in SEQ ID NO:97 with a substitution at one or two amino acid positions. In other embodiments, the isolated antibody or antigen-binding fragment thereof has a heavy chain CDR1 that comprises or consists of the amino acid sequence STYTMS (SEQ ID NO:90); a heavy chain CDR2 that comprises or consists of the amino acid sequence WVATISPGDSFGYY (SEQ ID NO:93); a heavy chain CDR3 that comprises or consists of the amino acid sequence TRDIYYNYGAWFA (SEQ ID NO:94); a light chain CDR1 that comprises or consists of the amino acid sequence DYDGDSYMNWY (SEQ ID NO:95); a light chain CDR2 comprises or consists of the amino acid sequence LLIYAASTLE (SEQ ID NO:96); and a light chain CDR3 that comprises or consists of the amino acid sequence QQANEDPR (SEQ ID NO:97).
In certain embodiments of the above aspects, the antibody has a human heavy chain and light chain constant region. In certain embodiments, the heavy chain constant region comprises a CH1 domain and a hinge region. In some embodiments, the heavy chain constant region comprises a CH3 domain. If the heavy chain constant region includes substitutions, such substitutions modify the properties of the antibody (e.g., increase or decrease one or more of: Fc receptor binding, antibody glycosylation, the number of cysteine residues, effector cell function, or complement function). In certain embodiments, the antibody is an IgG antibody. In specific embodiments, the antibody is selected from the group consisting of IgG1, IgG2, IgG3, and IgG4. In certain embodiments, the antibody includes a human Fc region that binds FcγRIIa (CD32a) with an EC50 of 7 to 15 μg/mL. In certain embodiments, the antibody includes a human Fc region that binds FcγRIIa (CD32a) with an EC50 of 10 μg/mL. In certain embodiments, the antibody includes a human Fc region that binds FcγRIIa (CD32a) with an EC50 of 11 μg/mL. In certain embodiments, the antibody includes a human Fc region that binds FcγRIIa (CD32a) with an EC50 of 12 μg/mL.
In another aspect, the disclosure features an isolated antibody or antigen-binding fragment thereof that selectively binds to the ectodomain of human BDCA2 (SEQ ID NO:1), and comprises a heavy chain CDR1, a heavy chain CDR2, and a heavy chain CDR3 of the VH set forth in any one of SEQ ID NOs: 40, 42, 44, 46, 49, or 52. In some embodiments of this aspect, isolated antibody or antigen-binding fragment thereof comprises a light chain CDR1, a light chain CDR2, and a light chain CDR3 of the VL set forth in any one of SEQ ID NOs: 54, 56, or 58. The CDRs can be the Kabat CDRs or any of the alternate CDRs. In certain embodiments, the antibody has a human heavy chain and light chain constant region. In certain embodiments, the heavy chain constant region comprises a CH1 domain and a hinge region. In some embodiments, the heavy chain constant region comprises a CH3 domain. If the heavy chain constant region includes substitutions, such substitutions modify the properties of the antibody (e.g., increase or decrease one or more of: Fc receptor binding, antibody glycosylation, the number of cysteine residues, effector cell function, or complement function). In certain embodiments, the antibody is an IgG antibody. In specific embodiments, the antibody is selected from the group consisting of IgG1, IgG2, IgG3, and IgG4. In certain embodiments, the antibody includes a human Fc region that binds FcγRIIa (CD32a) with an EC50 of 7 to 15 μg/mL. In certain embodiments, the antibody includes a human Fc region that binds FcγRIIa (CD32a) with an EC50 of 10 μg/mL. In certain embodiments, the antibody includes a human Fc region that binds FcγRIIa (CD32a) with an EC50 of 11 μg/mL. In certain embodiments, the antibody includes a human Fc region that binds FcγRIIa (CD32a) with an EC50 of 12 μg/mL. In another aspect, the disclosure features an isolated antibody or antigen-binding fragment thereof that selectively binds to the ectodomain of human BDCA2 (SEQ ID NO:1) and comprises a variable heavy chain (VH) domain that is at least 80% identical to the amino acid sequence of the VH domain of BIIB059 (SEQ ID NO:24), or the VH domain set forth in any one of SEQ ID NOs: 40, 42, 44, 46, 49, or 52. These antibodies (i) bind human or cynomolgus monkey BDCA2 but do not significantly bind BDCA2 from phylogenetic species below primates; and/or (ii) inhibit TLR7/TLR9-induced type I interferon and other cytokine or chemokine production by human pDCs; and/or (iii) mediate internalization of BDCA2 from the surface of pDCs; and/or (iv) downregulate CD32a and/or CD62L from the surface of pDCs; and/or (v) deplete pDCs in vitro by ADCC or CDC.
In certain embodiments of this aspect, the antibody or antibody fragment thereof comprises or consists of a VH domain that is at least 90% identical to the amino acid sequence of the VH domain of BIIB059 (SEQ ID NO:24), or the VH domain set forth in any one of SEQ ID NOs: 40, 42, 44, 46, 49, or 52. In some embodiments of this aspect, the antibody or antibody fragment thereof comprises or consists of a VH domain that is at least 95% identical to the amino acid sequence of the VH domain of BIIB059 (SEQ ID NO:24), or the VH domain set forth in any one of SEQ ID NOs: 40, 42, 44, 46, 49, or 52. In other embodiments of this aspect, the VH domain of the isolated antibody or antigen-binding fragment is identical to the amino acid sequence of the VH domain of BIIB059 (SEQ ID NO:24), or the VH domain set forth in any one of SEQ ID NOs: 40, 42, 44, 46, 49, or 52. In certain embodiments, the heavy chain comprises or consists of the amino acid sequence set forth in SEQ ID NO:4. In certain embodiments of this aspect, the antibody or antigen-binding fragment thereof comprises or consists of a variable light chain (VL) domain that is at least 80% identical to the amino acid sequence of the VL domain of BIIB059 (SEQ ID NO:23), or the VL domain set forth in any one of SEQ ID NOs: 54, 56, or 58. In some embodiments of this aspect, the antibody or antigen-binding fragment thereof comprises or consists of a VL domain that is at least 90% identical to the amino acid sequence of the VL domain of BIIB059 (SEQ ID NO:23), or the VL domain set forth in any one of SEQ ID NOs: 54, 56, or 58. In some embodiments of this aspect, the antibody or antigen-binding fragment thereof comprises or consists of a VL domain that is at least 95% identical to the amino acid sequence of the VL domain of BIIB059 (SEQ ID NO:23), or the VL domain set forth in any one of SEQ ID NOs: 54, 56, or 58. In some embodiments of this aspect, the antibody or antigen-binding fragment thereof comprises or consists of a VH domain that is identical to the amino acid sequence of the VH domain of BIIB059 (SEQ ID NO:24) and a VL domain that is identical to the amino acid sequence of the VL domain of BIIB059 (SEQ ID NO:23). In some embodiments of this aspect, the antibody or antigen-binding fragment thereof comprises or consists of a VH domain that is identical to the amino acid sequence of a VH domain set forth in any one of SEQ ID NOs: 40, 42, 44, 46, 49, or 52 and a VL domain set forth in any one of SEQ ID NOs: 54, 56, or 58. In a particular embodiment, the antibody or antigen-binding fragment thereof comprises or consists of a heavy chain that comprises or consists of the amino acid sequence set forth in SEQ ID NO:4 and a light chain that comprises or consists of the amino acid sequence set forth in SEQ ID NO:3. These embodiments relate to all of the above aspects and their embodiments. In certain embodiments, the antibody or antigen-binding fragment thereof is a humanized antibody. In some embodiments, the antibody or antigen-binding fragment thereof is a monoclonal antibody. In some embodiments, the antibody or antigen-binding fragment thereof is a single chain antibody. In other embodiments, the antibody or antigen-binding fragment is a polyclonal antibody, a chimeric antibody, an Fab fragment, an F(ab′)2 fragment, an Fab′ fragment, an Fsc fragment, an Fv fragment, an scFv, an sc(Fv)2, or a diabody. In some embodiments, the antibody has an IgG1 heavy chain constant region.
In another aspect, the disclosure provides an isolated antibody or antigen binding fragment thereof that selectively binds to the ectodomain of human BDCA2 (SEQ ID NO:1) and comprises a heavy chain CDR1, a heavy chain CDR2, and a heavy chain CDR3 of the antibody produced by the hybridoma deposited at the ATCC with the designation number PTA-13450. In certain embodiments of this aspect, the antibody or antigen binding fragment thereof further comprises a light chain CDR1, a light chain CDR2, and a light chain CDR3 of the antibody produced by the hybridoma deposited at the ATCC with the designation number PTA-13450. In certain embodiments of this aspect, the antibody has a human heavy chain and light chain constant region. In certain embodiments, the heavy chain constant region comprises a CH1 domain and a hinge region. In some embodiments, the heavy chain constant region comprises a CH3 domain. If the heavy chain constant region includes substitutions, such substitutions modify the properties of the antibody (e.g., increase or decrease one or more of: Fc receptor binding, antibody glycosylation, the number of cysteine residues, effector cell function, or complement function). In certain embodiments, the antibody is an IgG antibody. In specific embodiments, the antibody is selected from the group consisting of IgG1, IgG2, IgG3, and IgG4. In certain embodiments, the antibody includes a human Fc region that binds FcγRIIa (CD32a) with an EC50 of 7 to 15 μg/mL. In certain embodiments, the antibody includes a human Fc region that binds FcγRIIa (CD32a) with an EC50 of 10 μg/mL. In certain embodiments, the antibody includes a human Fc region that binds FcγRIIa (CD32a) with an EC50 of 11 μg/mL. In certain embodiments, the antibody includes a human Fc region that binds FcγRIIa (CD32a) with an EC50 of 12 μg/mL. In another aspect, the disclosure provides an isolated antibody or antigen binding fragment thereof that selectively binds to the ectodomain of human BDCA2 (SEQ ID NO:1) and comprises variant heavy chain CDR1, heavy chain CDR2, and heavy chain CDR3 of the antibody produced by the hybridoma deposited at the ATCC with the designation number PTA-13450, wherein the variant heavy chain CDR1, CDR2, and CDR3 includes one, two, or three amino acid substitutions compared to the heavy chain CDR1, CDR2, and CDR3, respectively, of the antibody produced by the hybridoma deposited at the ATCC with the designation number PTA-13450. In certain embodiments of this aspect, the antibody or antigen binding fragment thereof further comprises variant light chain CDR1, light chain CDR2, and light chain CDR3 of the antibody produced by the hybridoma deposited at the ATCC with the designation number PTA-13450, wherein the variant light chain CDR1, CDR2, and CDR3 includes one, two, or three amino acid substitutions compared to the light chain CDR1, CDR2, and CDR3, respectively, of the antibody produced by the hybridoma deposited at the ATCC with the designation number PTA-13450. In certain embodiments of this aspect, the antibody has a human heavy chain and light chain constant region. In certain embodiments, the heavy chain constant region comprises a CH1 domain and a hinge region. In some embodiments, the heavy chain constant region comprises a CH3 domain. If the heavy chain constant region includes substitutions, such substitutions modify the properties of the antibody (e.g., increase or decrease one or more of: Fc receptor binding, antibody glycosylation, the number of cysteine residues, effector cell function, or complement function). In certain embodiments, the antibody is an IgG antibody. In specific embodiments, the antibody is selected from the group consisting of IgG1, IgG2, IgG3, and IgG4. In certain embodiments, the antibody includes a human Fc region that binds FcγRIIa (CD32a) with an EC50 of 7 to 15 μg/mL. In certain embodiments, the antibody includes a human Fc region that binds FcγRIIa (CD32a) with an EC50 of 10 μg/mL. In certain embodiments, the antibody includes a human Fc region that binds FcγRIIa (CD32a) with an EC50 of 11 μg/mL. In certain embodiments, the antibody includes a human Fc region that binds FcγRIIa (CD32a) with an EC50 of 12 μg/mL. In another aspect, the disclosure features an isolated antibody or antigen binding fragment thereof that selectively binds to the ectodomain of human BDCA2 (SEQ ID NO:1) and crossblocks binding of the antibody produced by the hybridoma deposited at the ATCC with the designation number PTA-13450. In certain embodiments, the antibody is an IgG antibody. In specific embodiments, the antibody is selected from the group consisting of IgG1, IgG2, IgG3, and IgG4. In certain embodiments, the antibody includes a human Fc region that binds FcγRIIa (CD32a) with an EC50 of 7 to 15 μg/mL. In certain embodiments, the antibody includes a human Fc region that binds FcγRIIa (CD32a) with an EC50 of 10 μg/mL. In certain embodiments, the antibody includes a human Fc region that binds FcγRIIa (CD32a) with an EC50 of 11 μg/mL. In certain embodiments, the antibody includes a human Fc region that binds FcγRIIa (CD32a) with an EC50 of 12 μg/mL. In yet another aspect, the disclosure features an isolated antibody or antigen binding fragment thereof that selectively binds to the ectodomain of human BDCA2 (SEQ ID NO:1) at the same epitope as the antibody produced by the hybridoma deposited at the ATCC with the designation number PTA-13450. In certain embodiments, the antibody is an IgG antibody. In specific embodiments, the antibody is selected from the group consisting of IgG1, IgG2, IgG3, and IgG4. In certain embodiments, the antibody includes a human Fc region that binds FcγRIIa (CD32a) with an EC50 of 7 to 15 μg/mL. In certain embodiments, the antibody includes a human Fc region that binds FcγRIIa (CD32a) with an EC50 of 10 μg/mL. In certain embodiments, the antibody includes a human Fc region that binds FcγRIIa (CD32a) with an EC50 of 11 μg/mL. In certain embodiments, the antibody includes a human Fc region that binds FcγRIIa (CD32a) with an EC50 of 12 μg/mL. In a further aspect, the disclosure features an isolated antibody or antigen binding fragment thereof that selectively binds to the ectodomain of human BDCA2 (SEQ ID NO:1) and comprises a VH domain that is at least 75%, at least 80%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, or at least 98%, identical to the VH domain of the antibody produced by the hybridoma deposited at the ATCC with the designation number PTA-13450. In certain embodiments of this aspect, the isolated antibody or antigen binding fragment thereof comprises a VL domain that is at least 75%, at least 80%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, or at least 98%, identical to the VL domain of the antibody produced by the hybridoma deposited at the ATCC with the designation number PTA-13450.
In all of the above five aspects, the antibody or antigen binding fragment thereof further: (i) inhibits secretion of type I interferons and/or type III interferons in addition to other cytokines and chemokines from plasmacytoid dendritic cells; or (ii) induces or enhances depletion of plasmacytoid dendritic cells in vitro. In some embodiments of the above five aspects, the antibody downregulates CD32a and/or CD62L on a pDC (relative to a pDC that is not contacted with an anti-BDCA2 antibody). In certain embodiments, the antibody mediates internalization of BDCA2 from the surface of pDCs. In some embodiments of the above five aspects, the antibody or antigen-binding fragment thereof binds to cynomolgus BDCA2 (SEQ ID NO:72) and rhesus BDCA2 (SEQ ID NO:72). In certain embodiments of the above five aspects, the isolated antibody or antigen-binding fragment thereof inhibits secretion or production of type I interferon, interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), type III interferon, macrophage inflammatory protein-1 (MIP-1)-α/CCL3, MIP-1β/CCL4, chemokine (C—C motif) ligand 5 (CCL5/RANTES), or interferon γ-induced protein-10 (IP-10/CXCL10). In certain embodiments of the above five aspects, the antibody or antigen-binding fragment thereof is a humanized antibody. In some embodiments of the above five aspects, the antibody or antigen-binding fragment thereof is a monoclonal antibody. In some embodiments of the above five aspects, the antibody or antigen-binding fragment thereof is a single chain antibody. In other embodiments of the above five aspects, the antibody or antigen-binding fragment is a polyclonal antibody, a chimeric antibody, an Fab fragment, an F(ab′)2 fragment, an Fab′ fragment, an Fsc fragment, an Fv fragment, an scFv, an sc(Fv)2, or a diabody. In some embodiments of the above five aspects, the antibody has an IgG1 heavy chain constant region. In some embodiments of the above five aspects, the antibody has an IgG2 heavy chain constant region. In some embodiments of the above five aspects, the antibody has an IgG4 heavy chain constant region. In some embodiments of the above five aspects, the antibody is a hybrid of the IgG1 and IgG4 heavy chain constant regions.
In certain embodiments, the disclosure provides an isolated cell that produces any of the above-described antibodies or antigen-binding fragments thereof.
In other embodiments, the disclosure provides a pharmaceutical composition comprising any of the above-described antibodies or antigen-binding fragments thereof and a pharmaceutically acceptable carrier. In some embodiments, the pharmaceutical composition comprises any of the above described antibodies or antigen-binding fragments thereof formulated in a composition comprising 10-25 mM citrate, 100-200 mM sodium chloride, and a pH of 5.5-6.5. In certain embodiments the pharmaceutical composition optionally includes Tween-80 (0.01 to 0.3%, e.g., 0.03%). In yet other embodiments, the pharmaceutical composition comprises any of the above described antibodies or antigen-binding fragments thereof formulated in a composition comprising 20 mM sodium citrate, 150 mM sodium chloride, and a pH of 6.0.
In another aspect, the disclosure provides a method for making an anti-BDCA2 antibody. The method involves providing a cell comprising a heavy chain and/or a light chain of the BDCA2 antibody, incubating the cell under conditions that permit the expression of the antibody and isolating the antibody. The method optionally comprises purifying the antibody. In certain embodiments, the cell is a CHO cell. In other embodiments the cell is a 293 cell. In a particular embodiment, the anti-BDCA2 antibody is BIIB059. In one embodiment, the anti-BDCA2 antibody or antigen-binding fragment thereof has a heavy chain and light chain, wherein the heavy chain comprises or consists of the sequence set forth in SEQ ID NO:4, and the light chain comprises or consists of the sequence set forth in SEQ ID NO:3. In another embodiment, the anti-BDCA2 antibody or antigen-binding fragment thereof comprises or consists of a VH CDR1 comprising or consisting of the amino acid sequence of SEQ ID NO:9, a VH CDR2 comprising or consisting of the amino acid sequence of SEQ ID NO:10, and a VH CDR3 comprising or consisting of the amino acid sequence of SEQ ID NO:11. In a further embodiment, the anti-BDCA2 antibody or antigen-binding fragment thereof comprises or consists of a VH CDR1 comprising or consisting of the amino acid sequence of SEQ ID NO:9, a VH CDR2 comprising or consisting of the amino acid sequence of SEQ ID NO:10, a VH CDR3 comprising or consisting of the amino acid sequence of SEQ ID NO:11, a VL CDR1 comprising or consisting of the amino acid sequence of SEQ ID NO:5, a VL CDR2 comprising or consisting of the amino acid sequence of SEQ ID NO:6, and a VL CDR3 comprising or consisting of the amino acid sequence of SEQ ID NO:7.
In another aspect, the disclosure provides a method for detecting the presence of a plasmacytoid dendritic cell in a tissue. The method comprises contacting the tissue with an anti-BDCA2 antibody. In certain embodiments, the tissue is a skin biopsy from a subject having systemic lupus erythematosus. In certain embodiments, the tissue is a skin biopsy from a subject having scleroderma. In certain embodiments, the tissue is a skin biopsy from a subject having morphea. In certain embodiments, the tissue is a skin biopsy from a subject having rheumatoid arthritis. In certain embodiments, the tissue is a skin biopsy from a subject having psoriasis. In certain embodiments, the tissue is a skin biopsy from a subject having dermatomyositis. In certain embodiments, the tissue is a skin biopsy from a subject having polymyositis. In certain embodiments, the tissue is a skin biopsy from a subject having inflammatory bowel disease. In specific embodiments, the systemic lupus erythematosus is cutaneous lupus, discoid lupus, or lupus nephritis. The anti-BDCA2 antibody or antigen-binding fragment thereof may be labeled, e.g., with a fluorophore (e.g., Alexa Fluor 647). In certain embodiments, the anti-BDCA2 antibody is BIIB059. In other embodiments, the anti-BDCA2 antibody is clone 124B3.13 (Dendritics). In certain embodiments, the method further comprises contacting the tissue with an anti-CD123 antibody.
In another aspect, the disclosure provides a method of inducing death of a plasmacytoid dendritic cell in a subject in need thereof. The method involves administering to the subject, or contacting a plasmacytoid dendritic cell that expresses BDCA2 with, any of the antibodies or antigen-binding fragments thereof described herein.
In another aspect, the disclosure features a method of reducing production of inflammatory cytokines or chemokines by a plasmacytoid dendritic cell in a subject in need thereof. The method comprises administering to the subject, or contacting a plasmacytoid dendritic cell that expresses BDCA2 with, an effective amount of any of the antibodies or antigen-binding fragments thereof described herein. In certain embodiments, the inflammatory cytokines or chemokines are selected from the group consisting of: type I interferon, IL-6, or TNF-α, type III interferon, MIP-1α/CCL3, MIP-1β/CCL4, CCL5/RANTES, and IP-10/CXCL10.
In another aspect, the disclosure features a method of downregulating expression of CD32a on the surface of a plasmacytoid dendritic cell. The method comprises contacting the plasmacytoid dendritic cell with an anti-BDCA2 antibody described herein. In certain embodiments, the anti-BDCA2 antibody has an IgG1 heavy chain constant region. In some embodiments, the antibody has an IgG2 heavy chain constant region. In some embodiments, the antibody has an IgG4 heavy chain constant region. In some embodiments, the antibody is a hybrid of the IgG1 and IgG4 heavy chain constant regions. In certain embodiments, the antibody is aglycosylated. In a specific embodiment, the antibody is an aglycosylated hybrid of the IgG1 and IgG4 heavy chain constant regions.
In another aspect, the disclosure features a method of downregulating expression of CD32a (FcγRIIa) on the surface of a plasmacytoid dendritic cell in a human subject in need thereof. The method comprises administering to the human subject an effective amount of an anti-BDCA2 antibody described herein. In certain embodiments, the anti-BDCA2 antibody has an IgG1 heavy chain constant region. In some embodiments, the antibody has an IgG2 heavy chain constant region. In some embodiments, the antibody has an IgG4 heavy chain constant region. In some embodiments, the antibody is a hybrid of the IgG1 and IgG4 heavy chain constant regions. In certain embodiments, the antibody is aglycosylated. In a specific embodiment, the antibody is an aglycosylated hybrid of the IgG1 and IgG4 heavy chain constant regions.
In another aspect, the disclosure features a method of inhibiting stimulation of a plasmacytoid dendritic cell by immune complexes in a human subject in need thereof. The method comprises administering to the human subject an effective amount of an anti-BDCA2 antibody described herein. In some embodiments, the administration reduces the level of CD32a on the surface of pDCs. In some embodiments, the subject has Type III hypersensitivity. In one embodiment, the human subject has SLE. In another embodiment, the human subject has rheumatoid arthritis. In yet another embodiment, the subject has Sjögren's syndrome. In certain embodiments, the anti-BDCA2 antibody has an IgG1 heavy chain constant region. In some embodiments, the antibody has an IgG2 heavy chain constant region. In some embodiments, the antibody has an IgG4 heavy chain constant region. In some embodiments, the antibody is a hybrid of the IgG1 and IgG4 heavy chain constant regions.
In another aspect, the disclosure features a method of downregulating expression (or shedding) of CD62L (L-selectin) on the surface of a plasmacytoid dendritic cell in a human subject in need thereof. The method comprises administering to the human subject an effective amount of an anti-BDCA2 antibody or antigen-binding fragment described herein. In specific embodiments, the administration of the anti-BDCA2 antibody or antigen-binding fragment increases the level of one or more metalloproteinases. In certain embodiments, the downregulation of CD62L occurs through cleavage by a metalloproteinase. In certain embodiments, the anti-BDCA2 antibody has an IgG1 heavy chain constant region. In some embodiments of the above five aspects, the antibody has an IgG2 heavy chain constant region. In some embodiments of the above five aspects, the antibody has an IgG4 heavy chain constant region. In some embodiments of the above five aspects, the antibody is a hybrid of the IgG1 and IgG4 heavy chain constant regions.
In a further aspect, the disclosure features a method of treating an inflammatory disorder in a subject in need thereof. The method involves administering to the subject in need thereof an effective amount of any of the anti-BDCA2 antibodies or antigen-binding fragments thereof described herein. In some embodiments, the inflammatory disorder is selected from the group consisting of systemic lupus erythematosus (SLE), cutaneous lupus, discoid lupus, lupus nephritis, rheumatoid arthritis, inflammatory bowel disease, systemic sclerosis, morphea, psoriasis, type I diabetes, dermatomyositis, polymyositis, and Sjogren's disease. In one particular embodiment, the inflammatory disorder is SLE. In another particular embodiment, the inflammatory disorder is discoid lupus. In yet another particular embodiment, the inflammatory disorder is lupus nephritis. In another particular embodiment, the inflammatory disorder is cutaneous lupus. In certain embodiments, the subject has general SLE. In certain embodiments, the subject has moderate SLE. In certain embodiments, the subject has moderate SLE without severe active CNS and/or severe active renal involvement. In certain embodiments, the subject has moderate SLE with severe active CNS and/or severe active renal involvement. In certain embodiments, the subject has cutaneous manifestations of SLE (e.g., malar or discoid rash). In certain embodiments, the subject has severe SLE. In certain embodiments, the subject has severe SLE without severe active CNS and/or severe active renal involvement. In certain embodiments, the subject has severe SLE with severe active CNS and/or severe active renal involvement. Moderate or severe lupus is a staging of lupus (see, e.g., Guidelines for Referral and Management of Systemic Lupus Erythematosus in Adults, Arthritis & Rheumatism, 42(9):1785-1795 (1999); Gladman, Prognosis and treatment of systemic lupus erythematosus, Curr. Opin. Rheumatol., 8:430-437 (1996); Kalunian et al., Definition, classification, activity and damage indices. In: Dubois' lupus eyrthematosus. 5th ed., Baltimore: Williams and Wilkins; pp. 19-30 (1997)).
In another aspect, the disclosure features a method of treating an autoimmune disease in a subject in need thereof. The method involves administering to the subject in need thereof an effective amount of any of the anti-BDCA2 antibodies or antigen-binding fragments thereof described herein.
In any of the above aspects related to methods, in certain embodiments, the subject is a human. In any of the above aspects related to methods, in certain embodiments, the anti-BDCA2 antibody or antigen binding fragment is administered in combination with at least one of: an antimalarial (e.g., hydroxychloroquine), a TLR7 signaling inhibitor, a TLR9 signaling inhibitor, or a corticosteroid. In a specific embodiment, the anti-BDCA2 antibody comprises the heavy and light chain CDRs of BIIB059. In one embodiment, the anti-BDCA2 antibody comprises the heavy chain CDR1, CDR2, and CDR3 set forth in SEQ ID NOs. 8, 10, and 11, respectively and light chain CDR1, CDR2, and CDR3 set forth in SEQ ID NOs. 5, 6, and 7, respectively. In another embodiment, the anti-BDCA2 antibody comprises the heavy chain CDR1, CDR2, and CDR3 set forth in SEQ ID NOs. 89, 91, and 11, respectively and light chain CDR1, CDR2, and CDR3 set forth in SEQ ID NOs. 5, 6, and 7, respectively. In another embodiment, the anti-BDCA2 antibody comprises the heavy chain CDR1, CDR2, and CDR3 set forth in SEQ ID NOs. 9, 92, and 11, respectively and light chain CDR1, CDR2, and CDR3 set forth in SEQ ID NOs. 5, 6, and 7, respectively. In another embodiment, the anti-BDCA2 antibody comprises the heavy chain CDR1, CDR2, and CDR3 set forth in SEQ ID NOs. 90, 93, and 94, respectively and light chain CDR1, CDR2, and CDR3 set forth in SEQ ID NOs. 95, 96, and 97, respectively. In certain embodiments, the anti-BDCA2 antibody further comprises an Fc region which binds to CD32a with an EC50 of at least about 7 to 15 μg/mL (e.g., 10, 11, 12 μg/mL). In a specific embodiment, the anti-BDCA2 antibody is BIIB059.
In another aspect, the disclosure features a combination comprising an antimalarial (e.g., hydroxychloroquine) and an anti-BDCA2 antibody or antigen binding fragment thereof. In a specific embodiment, the anti-BDCA2 antibody comprises heavy chain CDRs (or alternate CDRs) of SEQ ID NO:24. In another embodiment, the anti-BDCA2 antibody comprises light chain CDRs (or alternate CDRs) of SEQ ID NO:23. In a specific embodiment, the anti-BDCA2 antibody comprises the heavy and light chain CDRs of BIIB059. In one embodiment, the anti-BDCA2 antibody comprises the heavy chain CDR1, CDR2, and CDR3 set forth in SEQ ID NOs. 8, 10, and 11, respectively and light chain CDR1, CDR2, and CDR3 set forth in SEQ ID NOs. 5, 6, and 7, respectively. In another embodiment, the anti-BDCA2 antibody comprises the heavy chain CDR1, CDR2, and CDR3 set forth in SEQ ID NOs. 89, 91, and 11, respectively and light chain CDR1, CDR2, and CDR3 set forth in SEQ ID NOs. 5, 6, and 7, respectively. In another embodiment, the anti-BDCA2 antibody comprises the heavy chain CDR1, CDR2, and CDR3 set forth in SEQ ID NOs. 9, 92, and 11, respectively and light chain CDR1, CDR2, and CDR3 set forth in SEQ ID NOs. 5, 6, and 7, respectively. In another embodiment, the anti-BDCA2 antibody comprises the heavy chain CDR1, CDR2, and CDR3 set forth in SEQ ID NOs. 90, 93, and 94, respectively and light chain CDR1, CDR2, and CDR3 set forth in SEQ ID NOs. 95, 96, and 97, respectively. In certain embodiments, the anti-BDCA2 antibody further comprises an Fc region which binds to CD32a with an EC50 of at least about 7 to 15 μg/mL (e.g., 9, 10, 11, 12, 13, 14 μg/mL). In a specific embodiment, the anti-BDCA2 antibody is BIIB059.
In another aspect, the disclosure features a combination comprising a TLR7 and/or TLR9 signaling inhibitor and an anti-BDCA2 antibody or antigen binding fragment thereof. In a specific embodiment, the anti-BDCA2 antibody comprises heavy chain CDRs (or alternate CDRs) of SEQ ID NO:24. In another embodiment, the anti-BDCA2 antibody comprises light chain CDRs (or alternate CDRs) of SEQ ID NO:23. In a specific embodiment, the anti-BDCA2 antibody comprises the heavy and light chain CDRs of BIIB059. In one embodiment, the anti-BDCA2 antibody comprises the heavy chain CDR1, CDR2, and CDR3 set forth in SEQ ID NOs. 8, 10, and 11, respectively and light chain CDR1, CDR2, and CDR3 set forth in SEQ ID NOs. 5, 6, and 7, respectively. In another embodiment, the anti-BDCA2 antibody comprises the heavy chain CDR1, CDR2, and CDR3 set forth in SEQ ID NOs. 89, 91, and 11, respectively and light chain CDR1, CDR2, and CDR3 set forth in SEQ ID NOs. 5, 6, and 7, respectively. In another embodiment, the anti-BDCA2 antibody comprises the heavy chain CDR1, CDR2, and CDR3 set forth in SEQ ID NOs. 9, 92, and 11, respectively and light chain CDR1, CDR2, and CDR3 set forth in SEQ ID NOs. 5, 6, and 7, respectively. In another embodiment, the anti-BDCA2 antibody comprises the heavy chain CDR1, CDR2, and CDR3 set forth in SEQ ID NOs. 90, 93, and 94, respectively and light chain CDR1, CDR2, and CDR3 set forth in SEQ ID NOs. 95, 96, and 97, respectively. In certain embodiments, the anti-BDCA2 antibody further comprises an Fc region which binds to CD32a with an EC50 of at least about 7 to 15 μg/mL (e.g., 10, 11, 12 μg/mL). In a specific embodiment, the anti-BDCA2 antibody is BIIB059.
In a further aspect, the disclosure features a combination comprising a corticosteroid and an anti-BDCA2 antibody or antigen binding fragment thereof. In a specific embodiment, the anti-BDCA2 antibody comprises heavy chain CDRs (or alternate CDRs) of SEQ ID NO:24. In another embodiment, the anti-BDCA2 antibody comprises light chain CDRs (or alternate CDRs) of SEQ ID NO:23. In a specific embodiment, the anti-BDCA2 antibody comprises the heavy and light chain CDRs of BIIB059. In one embodiment, the anti-BDCA2 antibody comprises the heavy chain CDR1, CDR2, and CDR3 set forth in SEQ ID NOs. 8, 10, and 11, respectively and light chain CDR1, CDR2, and CDR3 set forth in SEQ ID NOs. 5, 6, and 7, respectively. In another embodiment, the anti-BDCA2 antibody comprises the heavy chain CDR1, CDR2, and CDR3 set forth in SEQ ID NOs. 89, 91, and 11, respectively and light chain CDR1, CDR2, and CDR3 set forth in SEQ ID NOs. 5, 6, and 7, respectively. In another embodiment, the anti-BDCA2 antibody comprises the heavy chain CDR1, CDR2, and CDR3 set forth in SEQ ID NOs. 9, 92, and 11, respectively and light chain CDR1, CDR2, and CDR3 set forth in SEQ ID NOs. 5, 6, and 7, respectively. In another embodiment, the anti-BDCA2 antibody comprises the heavy chain CDR1, CDR2, and CDR3 set forth in SEQ ID NOs. 90, 93, and 94, respectively and light chain CDR1, CDR2, and CDR3 set forth in SEQ ID NOs. 95, 96, and 97, respectively. In certain embodiments, the anti-BDCA2 antibody further comprises an Fc region which binds to CD32a with an EC50 of at least about 7 to 15 μg/mL (e.g., 9, 10, 11, 12, 13, 14 μg/mL). In a specific embodiment, the anti-BDCA2 antibody is BIIB059.
Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, the exemplary methods and materials are described below. All publications, patent applications, patents, and other references mentioned herein are incorporated by reference in their entirety. In case of conflict, the present application, including definitions, will control. The materials, methods, and examples are illustrative only and not intended to be limiting.
Other features and advantages of the invention will be apparent from the following detailed description, and from the claims.



BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 is a schematic depiction of BDCA2 signaling in a plasmacytoid dendritic cell (see, Geijtenbeek et al., Nature Reviews Immunology, 9:465-479 (2009)).
FIG. 2 is a graph showing hu24F4 Hx/L1 variants binding to human BDCA2.
FIG. 3 is a graph showing hu24F4 Hx/L1 variants binding to cynomolgus BDCA2.
FIG. 4 is a schematic map of plasmid pJP009 that encodes the anti-BDCA2 light chain. The anti-BDCA2 light chain nucleic acid sequence is under transcriptional control of the hCMV IE promoter and the hGH polyadenylation sequences. The gene for aminoglycoside phosphotransferase (neomycin resistance) is under transcriptional control of the murine phosphoglycerine kinase (muPGK) promoter and polyadenylation sequences. The remaining sequences, including the gene for beta-lactamase are for propagation and selection in E. coli. 
FIG. 5 is a schematic map of plasmid pJP010 that encodes the anti-BDCA2 heavy chain. The anti-BDCA2 heavy chain nucleic acid sequence is under transcriptional control of the hCMV IE promoter and human growth hGH polyadenylation sequences. The gene for dihydrofolate reductase (dhfr) is under transcriptional control of the SV40E promoter and polyadenylation sequences. The remaining sequences, including the gene for beta-lactamase are for propagation and selection in E. coli. 
FIG. 6 is a line graph showing the binding of BIIB059 on cynomolgus (A) and human (B) plasmacytoid dendritic cells. Cynomologous monkey (A) or human (B) whole blood was incubated with varying concentrations of Alexa647 labeled BIIB059 antibody (circles), or a human IgG isotype (squares) on ice. Data was acquired using the LSRII-4 color FACS machine, and analyzed using FlowJo and GraphPad Prism software.
FIG. 7 is a line graph showing the results of an AlphaScreen assay for self-association. Key: diamond=BIIB059; square=5c8; and triangle=LT105.
FIG. 8 is a line graph showing the results of a differential scanning fluorometry to test the stability of BIIB059 over different conditions. This graph shows data with 150 mM sodium chloride and 250 mM sucrose as a function of pH.
FIG. 9 is a line graph showing the effect of agitation on aggregation over time. Aggregation was suppressed with the addition of Tween 80.
FIG. 10 is a line graph showing direct binding of AC144 to human and cynomolgus surface BDCA2.
FIG. 11 is a series of graphs showing the results of size exclusion chromatography analysis of Fc fusion proteins.
FIG. 12 is a graph showing the effect of calcium on BIIB059 binding to BDCA2. BIIB059 binding to BDCA2 is enhanced by the addition of calcium relative to EDTA giving about a 2-fold higher signal.
FIG. 13 is a graph showing the results of Octet binding of BIIB059 to human and cynomolgus monkey BDCA2 ectodomains.
FIG. 14 is a graph showing that BIIB059 potently inhibits IFNα from PBMCs stimulated with TLR9 agonist. Each symbol represents IC50 from an independent experiment and vertical lines depict the SEM.
FIG. 15A-C provide a series of graphs showing that BIIB059 potently inhibits cytokines and chemokines from whole blood stimulated with TLR9 ligand. FIG. 15A shows inhibition of IFNα using heparinized venous blood from healthy donors. FIG. 15B shows inhibition of IFNα using whole blood from two SLE patients (upper panels) compared to results using whole blood from 2 healthy donors (bottom panels). FIG. 15C provides a series of bar graphs showing that BIIB059 treatment led to inhibition of a large array of cytokines and chemokines.
FIG. 16 is a bar graph showing that BIIB059 inhibits expression of type I interferon.
FIG. 17 includes two line graphs showing that ligation of BDCA2 with BIIB059 inhibits TLR9-induced cytokine production in purified pDCs.
FIG. 18 is a bar graph showing that ligation of BDCA2 suppresses induction of IFN-α production in SLE serum stimulated pDCs.
FIG. 19A is a line graph showing that BDCA2 is internalized after ligation with BIIB059. FIG. 19B is a line graph showing that internalization does not affect BIIB059-mediated inhibition of IFN-α production.
FIG. 20 is a series of line graphs showing BIIB059 binding to Fcγ receptors.
FIG. 21 provides the results of C1q ELISA showing binding of human C1q to increasing concentrations (0-15 μg/mL) of coated antibody.
FIG. 22A-D are a series of graphs showing that BIIB059 mediates cell killing through ADCC. The CHO cell line (EAG2456 T1F2 Clone 34.16.7) was used as the target cell. Expression level of BDCA2 on the surface of CHO cells was determined by FACS using an APC-labeled anti-BDCA2 mAb (clone AC144, Miltenyi). NK cells were used as the effector cells. ADCC was evaluated using the Vybrant Cytotoxicity Assay kit (Invitrogen), following the manufacturer's instructions. The assay detects G6PD from damaged cells based on the G6PD-dependent reduction of resazurin which emits fluorescence at 590 nm after excitation at 530 nm. The ADCC assay depicted in FIG. 22A was performed using high BDCA2 expressing CHO cells (FIG. 22C) while the ADCC assay in FIG. 22B used CHO cells with lower BDCA2 expression (FIG. 22D).
FIG. 23 is a line graph showing that BIIB059 mediates cell killing through CDC. CHO cells (EAG2456 T1F2 Clone 34.16.7) were seeded at 5×104 cells in 96 well Collagen black well plates and incubated at 370 C for 48 hrs. The plates were then washed and incubated with rabbit serum complement and propidium iodide (PI) in the presence of effector competent anti-BDCA2 mAbs (24F4S and BIIB059), effector function deficient mAbs (24F4S-Agly and 24F4A-Agly) or IgG1 isotype control for 1 h at 37° C. Negative control consisted of wells containing CHO cells, rabbit serum complement, PI, without antibodies.
FIG. 24 is a series of graphs used to determine EC50 of BIIB059 binding (“direct”) and competitive BIIB059-A647 binding (“indirect”) on cynomolgus monkey pDCs. Prior to in vivo injection of BIIB059, blood was drawn from twelve cynomolgus monkeys once a week for three weeks total. Flow cytometry was used to determine the EC50 of BIIB059 binding to BDCA2 on the pDC cell surface (“direct” method), and the amount of available BDCA2 receptor available in the presence of BIIB059 (“indirect” method). Blood was incubated with a six-point titration of BIIB059 at a range of 40-0.04 μg/mL. pDCs were identified by flow cytometry as CD20−CD14−CD123+HLA-DR+, and treated with either an anti-human IgG PE labeled secondary, or BIIB059-A647 labeled at 10 ug/mL. The MFI of PE (open symbols, graphed on the left y-axis) or A647 (closed symbols, graphed on the right y-axis) was calculated in FlowJo software, and graphed using GraphPad Prism software (four-parameter nonlinear regression curve fit of log-transformed data). Representative graphs from four of the twelve cynomolgus monkeys are shown here.
FIG. 25 is a representative graph presenting plateau binding of the anti-BDCA2 antibody BIIB059 to cell surface BDCA2 on pDCs in cynomolgus monkey whole blood. Blood was incubated with a six-point titration of BIIB059 at a range of 40-0.04 μg/mL. pDCs were identified by flow cytometry as CD20−CD14−CD123+HLA-DR+, and treated with an anti-human IgG PE labeled secondary. The MFI of PE was calculated in FlowJo software, and the percent of maximal binding, using the 40 μg/mL point as 100%, was computed. Each line represents one individual cynomolgus monkey, for a total of twelve cynomolgus monkeys, and graphed using GraphPad Prism software (four-parameter nonlinear regression curve fit of log-transformed data). Staining was repeated once a week for three weeks total. Dashed lines demonstrate that a concentration of 10 μg/mL of BIIB059 saturates BDCA2 receptor binding for all cynomolgus monkeys.
FIG. 26A-C addresses the levels of bound BIIB059 and free BDCA2 staining on vehicle treated cynomolgus monkeys. FIG. 26A is a series of FACS histograms showing background PE staining on vehicle treated cynomolgus monkeys. Cynomolgus monkeys 1, 4 and 12 were administered a single IV injection of vehicle control (sodium citrate) at time 0. After 1 hour, whole blood was drawn, and pDCs were identified by flow cytometry as CD20-CD14−CD123+HLA-DR+, and treated with anti-human IgG PE (open histograms) or FACS buffer as PE fluorescence minus one (FMO) control (solid histograms). FIG. 26B is a graph of PE staining on pDCs from blood draws from the three vehicle treated cynomolgus monkeys at the indicated time points. The MFI of PE was calculated in FlowJo software, and graphed using GraphPad Prism software. FIG. 26C is a graph of A647 staining on pDCs from blood draws from the three vehicle treated cynomolgus monkeys at the indicated time points. BIIB059-A647 at 10 μg/mL was added to the blood draws from the three vehicle treated cynomolgus monkeys at each of the indicated time points, and assayed for A647 staining on pDCs. The MFI of A647 was calculated in FlowJo software, and graphed using GraphPad Prism software.
FIG. 27A-C show that bound BIIB059 and BDCA2 receptor are no longer available on pDC cell surface after a single dose of BIIB059 10 mg/kg in cynomolgus monkey. FIG. 27A is a series of FACS histograms showing BIIB059 staining on BIIB059 10 mg/kg treated cynomolgus monkeys. Cynomolgus monkeys 3, 8 and 10 were administered a single IV injection of BIIB059 at 10 mg/kg at time 0. After 1 hour, whole blood was drawn, and pDCs were identified as CD20−CD14−CD123+HLA-DR+, and treated with anti-human IgG PE (open histograms) or FACS buffer as PE FMO control (solid histograms). FIG. 27B is a graph of PE staining on pDCs from blood draws from the three BIIB059 treated cynomolgus monkeys at the indicated time points. The MFI of PE was calculated in FlowJo software, and graphed using GraphPad Prism software. FIG. 27C is a graph of A647 staining on pDCs from blood draws from the three BIIB059 treated cynomolgus monkeys at the indicated time points. BIIB059-A647 at 10 μg/mL was added to the blood draws from the three BIIB059 treated cynomolgus monkeys at each of the indicated time points, and assayed for A647 staining on pDCs. The MFI of A647 was calculated in FlowJo software, and graphed using GraphPad Prism software.
FIG. 28A-C show that bound BIIB059 and BDCA2 receptor are no longer available on pDC cell surface after a single dose of BIIB059 1 mg/kg in cynomolgus monkey. FIG. 28A is a series of FACS histograms showing BIIB059 staining on BIIB059 1 mg/kg treated cynomolgus monkeys. Cynomolgus monkeys 3, 8 and 10 were administered a single IV injection of BIIB059 at 1 mg/kg at time 0. After 1 hour, whole blood was drawn, and pDCs were identified as CD20−CD14−CD123+HLA-DR+, and treated with anti-human IgG PE (open histograms) or FACS buffer as PE FMO control (solid histograms). FIG. 28B is a graph of PE staining on pDCs from blood draws from the three BIIB059 treated cynomolgus monkeys at the indicated time points. The MFI of PE was calculated in FlowJo software, and graphed using GraphPad Prism software. FIG. 28C is a graph of A647 staining on pDCs from blood draws from the three BIIB059 treated cynomolgus monkeys at the indicated time points. BIIB059-A647 at 10 μg/mL was added to the blood draws from the three BIIB059 treated cynomolgus monkeys at each of the indicated time points, and assayed for A647 staining on pDCs. The MFI of A647 was calculated in FlowJo software, and graphed using GraphPad Prism software.
FIG. 29A-C show that bound BIIB059 and BDCA2 receptor are no longer available on pDC cell surface after a single subcutaneous (SC) dose of BIIB059 0.2 mg/kg in cynomolgus monkey. FIG. 29A is a series of FACS histograms showing BIIB059 staining on SC 0.2 mg/kg BIIB059 treated cynomolgus monkeys. Cynomolgus monkeys 4, 6 and 12 were administered a single SC injection of BIIB059 at 0.2 mg/kg at time 0. After 1 hour, whole blood was drawn, and pDCs were identified as CD20−CD14−CD123+HLA-DR+, and treated with anti-human IgG PE (open histograms) or FACS buffer as PE FMO control (solid histograms). FIG. 29B is a graph of PE staining on pDCs from blood draws from the three BIIB059 treated cynomolgus monkeys at the indicated time points. The MFI of PE was calculated in FlowJo software, and graphed using GraphPad Prism software. FIG. 29C is a graph of A647 staining on pDCs from blood draws from the three BIIB059 treated cynomolgus monkeys at the indicated time points. BIIB059-A647 at 10 μg/mL was added to the blood draws from the three BIIB059 treated cynomolgus monkeys at each of the indicated time points, and assayed for A647 staining on pDCs. The MFI of A647 was calculated in FlowJo software, and graphed using GraphPad Prism software.
FIG. 30A-C are a series of graphs showing the observed PK/PD correlations for cynomolgus monkeys that received BIIB059 IV at 1 mg/kg, and cynomolgus monkeys that received BIIB059 IV at 10 mg/kg. For each graph in this figure, BIIB059 serum concentration is plotted on the left y-axis (open symbols), and BDCA2 receptor density is plotted on the right y-axis (solid symbols). The accelerated clearance observed in cynomolgus monkey 5 was likely due to immunogenicity to BIIB059.
FIG. 31A-B are a series of graphs showing the observed PK/PD correlations for cynomolgus monkeys that received BIIB059 SC at 0.2 mg/kg. For each graph in this figure, BIIB059 serum concentration is plotted on the left y-axis (open symbols), and BDCA2 receptor density is plotted on the right y-axis (solid symbols).
FIG. 32 is series of bar graphs showing the results of ELISA or multiplex assays to measure concentrations of inflammatory cytokines and chemokines produced by pDCs treated with CpG-A, CpG-A in the presence of anti-BDCA2, and CpG-A in the presence of isotype control. Each bar represents the mean and standard deviation (SD) for duplicate wells from a representative healthy human donor out of 5 tested. Vertical lines depict the SD.
FIG. 33 is series of bar graphs showing the results of ELISA or multiplex assays to measure concentrations of inflammatory cytokines and chemokines produced by pDCs treated with Sm/RNP immune complexes, Sm/RNP immune complexes in the presence of anti-BDCA2, and Sm/RNP immune complexes in the presence of isotype control. Each bar represents the mean and standard deviation (SD) for duplicate wells from a representative healthy human donor out of 5 tested. Vertical lines depict the SD.
FIG. 34 is a series of bar graphs showing the results of qPCR assays to determine the effect of BIIB059 on the transcription of type I IFN subtypes in Sm/RNP IC stimulated pDCs from healthy human donors. Each bar represent the mean relative fold change for quadruplicate wells from a representative donor out of 3 tested (n=3) and vertical lines depict the standard deviation (SD).
FIG. 35A shows BIIB059-mediated dose dependent inhibition of TLR9-induced IFNα by PBMC from one representative healthy human donor out of 18 tested. Each symbol represents the mean and standard deviation (SD) for duplicate wells. FIG. 35B shows BIIB059-mediated dose dependent inhibition of TLR9-induced IFNα by PBMC from one representative SLE patient out of 11 tested. Each symbol represents the mean and standard deviation (SD) for duplicate wells. FIG. 35C shows IC50 values for BIIB059 inhibition of TLR9-induced IFNα production by PBMC in healthy human donors (HD) compared to SLE patients (SLE). Each symbol represents an individual donor and vertical lines depict the SD.
FIG. 36A shows BIIB059-mediated dose dependent inhibition of TLR9-induced IFNα from one representative whole blood assay out of 12 tested. Each symbol represents the mean and standard deviation (SD) for duplicate wells. FIG. 36B shows IC50 values for BIIB059 inhibition of TLR9-induced IFNα production in whole blood assays compared with PBMC assays. Each symbol represents an individual donor and vertical lines depicts the SD.
FIG. 37 PBMC from healthy human donors were stimulated with 1 μM of the TLR3 ligand (Poly I:C) and treated with concentrations of BIIB059 ranging from 10 μg/mL to 0.5 ng/mL in a total assay volume of 250 μL/well in a 96 well plate. The plates were incubated overnight (18 hours) at 37° C. and 5% CO2. 200 μL of the supernatants were collected for evaluation of IFNα levels by ELISA. Each symbol represents the average IFNα levels produced at each treatment condition. Data from two independent donors are shown. Vertical lines depict the standard deviation (SD).
FIG. 38A shows dose dependent BIIB059-mediated BDCA2 internalization from a representative healthy human donor. Circles represent MFI of 2D6 staining at the various doses of BIIB059. Triangle represents the MFI of 2D6 in presence of the isotype control (maximum staining). Diamond represents the MFI of FMO control (background staining). FIG. 38B shows EC50 of BIIB059-induced BDCA2 internalization on pDCs in whole blood assays from healthy human donors (closed circles; n=10 donors). The average EC50 was 0.017±0.005 μg/mL.
FIG. 39 is a graphical depiction of mean fluorescence intensity (MFI) values of 2D6-FITC staining of gated CD14−CD20−HLA-DR+CD123+pDCs. Isotype (iso) represents the maximum staining, FMO (fluorescence minus one control) consisted of the FACS staining cocktail minus 2D6-FITC represent background staining. Shown in this figure is a representative experiment of 4 independent experiments performed.
FIG. 40 are confocal images of human pDCs purified from peripheral blood and then incubated with 10 μg/mL of BIIB059-AF647 (white) at 4° C. (left) or at 37° C. in 5% CO2 (right) for 15 min. BIIB059 cell distribution was assessed by confocal microscopy, and a representative picture is shown for each condition.
FIG. 41 is a graphical depiction of the effect of internalization of BDCA2 on inhibition of IFNα production. This figure is a representative of 3 independent experiments.
FIG. 42 is a graphical depiction showing that the EC50 values of BIIB059-mediated BDCA2 internalization correlated with IC50 values of BIIB059-mediated inhibition of TLR9-induced IFNα in whole blood assays (n=10). R2 value of 0.57.
FIG. 43A shows results expressed as the mean and standard deviation (SD) of the Manders colocalization coefficients for TLR9 localization in the LAMP1+ compartment. FIG. 43B shows results expressed as the mean and SD of the Manders colocalization coefficients for BIIB059/BDCA2 localization in the TLR9+ compartment. FIG. 43C shows results expressed as the mean and SD of the Manders colocalization coefficients for BIIB059/BDCA2 localization in the LAMP1+ compartment. Each symbol represents an individual cell; horizontal lines represents the mean, vertical lines represents the SD.
FIG. 44A is a histogram from a representative experiment of whole blood treated with 10 μg/mL of BIIB059 (tinted histogram), 10 μg/mL isotype control (dotted line) or whole blood stimulated with the TLR9 ligand, CpG-A (solid line). FIG. 44B is a graphical depiction of the effect of BIIB059 treatment of whole blood resulted on shedding of CD62L (closed squares). The open square represents the isotype treatment (10 μg/mL). This figure is representative of 3 independent experiments.
FIG. 45 is a graphical depiction of the surface expression of CD62L was assayed by flow cytometry. CD62L expression was measured in the presence of BIIB059 alone, and with increasing concentrations of GM6001 (circles). The open square represents the isotype treated control (10 μg/mL). Inverted triangle represents BIIB059 treated DMSO control. This figure is representative of 2 independent experiments.
FIG. 46A is a graphical depiction of BIIB059 and 24F4A-Agly mediated dose-dependent internalization of BDCA2 on the surface of pDCs from one representative healthy human donor (n=5). pDCs from human healthy donors were isolated using a two-step magnetic bead separation procedure (MACS kit, Miltenyi Biotec). pDCs were treated with increasing concentrations of BIIB059 (circles) or the a-glycosylated form of the antibody-24F4-Agly-(squares). Cells were also treated with 10 μg/mL of an isotype control (triangle) and incubated for 16 hours at 37° C. pDCs were then stained for surface expression of BDCA2 and CD32. FIG. 46B is a histogram showing levels of CD32 on isolated pDCs treated with 10 μg/mL of BIIB059 (shaded) or the isotype control (dotted) (n=5). FIG. 46C is a histogram showing CD32 levels on isolated pDCs treated with 10 μg/mL of the a-glycosylated form-24F4-A (shaded) or the isotype control (dotted). Solid line represents the unstained cells (n=5). FIG. 46D is a graphical depiction of BIIB059-mediated dose dependent down-modulation of CD32 on the surface of pDCs from one representative healthy human donor (n=5). FIG. 46E is a histogram showing levels of CD32 on isolated pDCs treated for 1 hour at 4° C. in the presence of 10 μg/mL of BIIB059 (shaded), the a-glycosylated form (dashed), or an isotype control (dotted). After incubation pDCs were assessed for CD32 surface expression. Solid black line represents unstained cells (n=3). FIG. 46F is a histogram showing levels of CD32 on isolated pDCs treated for 1 hour at 37° C. in the presence of 10 μg/mL of BIIB059 (shaded), the a-glycosylated form (dashed), or an isotype control (dotted). After incubation pDCs were assessed for CD32 surface expression. Solid black line represents unstained cells (n=3).
FIG. 47A is a graphical depiction of IFNα levels from isolated pDCs treated with increasing concentrations of BIIB059 (squares), increasing concentrations of the a-glycosylated form of the antibody 24F4-A (circles), or istotype control at 10 μg/mL (triangle). pDCs were stimulated in the presence of CpG-A (75 μg/mL) or left unstimulated (inverted triangle). pDCs were incubated for 16 hours at 37° C. and supernatants were collected and assayed for IFNα by ELISA. Shown is representative experiment out of 2 conducted. FIG. 47B is a graphical depiction of IFNα levels from isolated pDCs treated with increasing concentrations of BIIB059 (squares), increasing concentrations of the a-glycosylated form of the antibody 24F4-A (circles), istotype control at 10 μg/mL (triangle), or anti-human CD32 mAb at 10 μg/mL. Sm/RNP immune complexes (IC) were pre-formed by mixing Sm-RNP from calf thymus and anti-RNP antibodies purified form sera of SLE patients for 30 minutes in serum-free medium. Isolated cells were stimulated with immune complexes or treated with antigen alone (unstimulated). Cells were incubated for 16 hours at 37° C. and supernatants were collected and assayed for IFNα by ELISA. Shown is a representative figure of 3 conducted. Each symbol represents the mean and standard deviation (SD) for duplicate wells.
FIG. 48A is a bar graph showing CD32 expression on isolated pDCs treated with immune complexes in the presence of 10 μg/mL of BIIB059, 24F4-A, anti CD32 mAb (AT10 clone), humanized anti CD40 antibody, or isotype control. Cells were incubated for 16 hours at 37° C. pDCs were stained for surface expression of CD32 and CD40. FIG. 48B is a bar graph depicting IFNα levels measured by ELISA in the supernatants collected from A. Shown is a representative figure (n=3). FIG. 48C is a histogram showing CD40 expression on the surface of pDCs. The dotted line represents CD40 expression on the cell surface. The tinted histogram represents levels of CD40 on pDCs after treatment with anti-CD40 antibody. The solid line represents unstained cells.
FIG. 49 depicts the impact of HCQ on BIIB059 potency. Each symbol represents IFNα concentrations measured from an individual healthy human donor and vertical lines depict the SD. PBMC from healthy human donors were treated with varying concentrations of BIIB059 alone, HCQ alone or in combination (BIIB059+HCQ) in a total assay volume of 250 μL/well. Concentrations of BIIB059 ranged from 10 μg/mL to 0.1 μg/mL. Concentrations of HCQ ranged from 10 μM to 156 nM. 1×106 PBMC cells/well were stimulated with 5 μM of the TLR7 ligand (R848). The plates containing PBMC were incubated overnight (18 hours) at 37° C. and 5% CO2. 200 μL of the supernatants were collected for evaluation in IFNα ELISA (PBL InterferonSource).
FIG. 50 depicts the impact of HCQ on BIIB059 potency. Each symbol represents IFNα concentrations measured from a representative donor of 2 tested healthy donors and vertical lines depict the standard deviation (SD). PBMC from heparinized venous blood of healthy human donors or SLE patients were isolated by discontinuous gradient centrifugation over Ficoll, washed in PBS and resuspended in complete culture medium (RPMI with 3% FBS). PBMC were treated with varying concentrations of BIIB059 alone, HCQ alone or in combination (BIIB059+HCQ) in a total assay volume of 250 μL/well. Concentrations of BIIB059 ranged from 10 μg/mL to 0.1 μg/mL. Concentrations of HCQ ranged from 10 μM to 156 nM. 1×106 PBMC cells/well were stimulated with 1 μM of the TLR9 ligand (CPG-A). The plates containing PBMC were incubated overnight (18 hours) at 37° C. and 5% CO2. 200 μL of the supernatants were collected for evaluation in IFNα ELISA (PBL InterferonSource).
FIG. 51 shows distributions of percent circulating pDC in healthy cynomolgus monkey whole blood on original scale (left panel) and on log scale (right panel). Whole blood was drawn from twelve cynomolgus monkeys once a week for four weeks total. pDCs were identified using flow cytometry as CD20−CD14−CD123+HLA-DR+. pDC as a percent of CD20−CD14− cells was calculated with FlowJo software. Graph was obtained using the R language for statistical computing.
FIG. 52 is a graphical depiction of the percent circulating pDC (on log scale) in healthy cynomolgus monkey whole blood by different time points prior to IV injection of BIIB059. At indicated time points, whole blood was drawn, and pDCs were identified by flow cytometry as CD20−CD14−CD123+HLA-DR+. Percent pDCS was calculated in FlowJo software, and graphed using R software.
FIG. 53 is a depiction of a final fitted model for percent circulating pDC (on log scale) in healthy cynomolgus monkey whole blood by different time points prior to IV injection of BIIB059. A linear mixed effects model for log (% pDC) values with different time points as the fixed factors and cynos as the random intercepts shows no differences among the ratios of the geometric means % pDC values measured by difference weeks (p-value based on F-test for all time effects equal to zero is 0.67). Graph and statistical analysis were calculated using the R language for statistical computing. The black line shows the final fitted model, which only includes a fixed intercept and the random intercepts for cynomolgus monkeys. lme4 package in R was used to fit the linear mixed effects model.
FIG. 54 depicts percent circulating pDC on log scale before and after IV dose of sodium citrate vehicle, BIIB059 1 mg/kg or BIIB059 10 mg/kg in cynomolgus monkey. Three cynomolgus monkeys were administered for each dose group at time 0. At indicated time points, whole blood was drawn, and pDCs were identified by flow cytometry as CD20−CD14−CD123+HLA-DR+. Percent pDCs was calculated in FlowJo software, and graphed using R software.
FIG. 55 depicts final fitted model for percent circulating pDC on log scale before and after IV dose of sodium citrate vehicle, BIIB059 1 mg/kg and BIIB059 10 mg/kg in cynomolgus monkeys. A linear mixed effects model for log (% pDC) values with fixed factors for dose group, time levels 1 hour, 6 hours and greater than 28 days, and with random intercept for cynomolgus monkeys. The solid line shows the fitted model. lme4 package in R was used to fit the linear mixed effects model. Graph and statistical analysis were calculated using the R language for statistical computing.
FIG. 56 shows percent circulating pDC after SC dose of BIIB059 0.2 mg/kg in cynomolgus monkey. Cynomolgus monkeys 4, 6 and 12 were administered a single SC injection of BIIB059 0.2 mg/kg at time 0. Out of the three cynomolgus monkeys, cynomolgus monkey 6 was dosed with BIIB059 mg/kg in previous study. Cynomolgus monkeys 4 and 12 were dosed with vehicle in previous study. At indicated time points, whole blood was drawn, and pDCs were identified by flow cytometry as CD20−CD14−CD123+HLA-DR+. Percent pDCs was calculated in FlowJo software, and graphed using R software.
FIG. 57 depicts the final fitted model for percent circulating pDC after SC dose of BIIB059 0.2 mg/kg in cynomolgus monkey. A linear mixed effects model is fitted for log (% pDC) values with fixed effects for continuous time and time at 1 hour, and with cynomolgus monkeys as random intercepts. The solid line shows the fitted model. lme4 package in R was used to fit the linear mixed effects model. Graph and statistical analysis were calculated using the R language for statistical computing.
FIG. 58 is a schematic representation of the Cynomolgus Monkey PK/PD Experimental Design. Nine Cynomolgus monkeys completed the intravenous (IV) dose study. Cynomolgus monkeys were bled before and after IV administration of vehicle, 1 mg/kg BIIB059, or 10 mg/kg BIIB059 according to the bleeding schedule shown. Following the completion of this study, 3 cynomolgus monkeys went on to complete a subcutaneous (SC) dose study, where they received a single SC injection of 0.2 mg/kg BIIB059. At each bleeding time point, a whole blood assay was performed where whole blood from the cynomolgus monkeys was diluted 1:4 with complete RPMI 1640 and stimulated with CPG-A to a final concentration of 200 μg/ml in a 96 well round bottom tissue culture plate and incubated at 37° C. 5% CO2 for 18-20 hours. At the end of the culture, the stimulated whole blood was centrifuged to harvest serum. In the MxA bioassay, A549 cells were stimulated with the harvested serum for 19-20 hours at 37° C. 5% CO2 to induce MxA protein. After 20 hours, A549 cells were lysed and a sandwich ELISA was performed to detect concentrations of MxA protein. IFNα levels (units/mL) were back calculated from a standard curve generated by treating A549 cells with increasing doses of rIFNα.
FIG. 59 is a graphical representation of the trend towards reduced TLR9-induced IFNα production in cynomolgus monkeys receiving a single intravenous dose of BIIB059 relative to pre-treatment averages. Whole blood from cynomolgus monkeys treated with a single intravenous dose of vehicle, 1 mg/kg BIIB059, or 10 mg/kg BIIB059 was diluted 1:4 with complete RPMI 1640 and stimulated with CPG-A (2216) to a final concentration of 200 μg/ml in a 96 well round bottom tissue culture plate and incubated at 37° C. 5% CO2 for 18-20 hours. At the end of the culture, the stimulated whole blood was centrifuged to harvest serum. A549 cells were stimulated with the harvested serum for 19-20 hours at 37° C. 5% CO2 to induce MxA protein. After 20 hours, A549 cells were lysed and a sandwich ELISA was performed to detect concentrations of MxA protein. IFNα levels (units/mL) were back calculated from a standard curve generated by treating A549 cells with increasing doses of rIFNα. The mean pre-bleed IFNα concentration was calculated for each monkey by averaging all IFNα measurements from the pre-bleed timepoints (Days −21, −14, −7 and T0). The % IFNα was then calculated for each bleeding timepoint following BIIB059 administration up to day 14 by dividing the concentration of IFNα at that time by the pre-bleed average for that animal and multiplying by 100. These values were then averaged for each treatment group. Graph depicts mean±standard error of the mean. Graph and statistical analysis were calculated using Excel and GraphPad 6.0 software (GraphPad, San Diego, Calif.).
FIG. 60 is a graphical depiction of the decreased TLR9-Induced IFNα production in an ex vivo Whole Blood Assay from cynomolgus monkeys treated intravenously with BIIB059. Whole blood from cynomolgus monkeys treated with a single intravenous dose of vehicle (top panel), 1 mg/kg BIIB059 (middle panel), or 10 mg/kg BIIB059 (bottom panel) was diluted 1:4 with complete RPMI 1640 and stimulated with CPG-A (2216) to a final concentration of 200 μg/ml in a 96 well round bottom tissue culture plate and incubated at 37° C. 5% CO2 for 18-20 hours. At the end of the culture, the stimulated whole blood was centrifuged to harvest serum. A549 cells were stimulated with the harvested serum for 19-20 hours at 37° C. 5% CO2 to induce MxA protein. After 20 hours, A549 cells were lysed and a sandwich ELISA was performed to detect concentrations of MxA protein. IFNα levels (Units/mL) were back calculated from a standard curve generated by treating A549 cells with increasing doses of rIFNα. A two-way mixed effects analysis of variance (ANOVA) was fit to log 10 values of the calculated concentrations of IFNα. IFNα values are plotted (on log 10 scale) versus day of bleed for each animal within each dose group. Vertical lines denote groupings of bleed days into pre-dose, post-dose up to 31 days, and post-dose greater than 31-days. Bleed days later than day 31 were not used in the analysis. The model-based estimates of geometric mean IFNα values are represented by thick black horizontal lines within the pre- and post-dose regions of each panel. Graph and statistical analysis were calculated using the R language for statistical computing.
FIG. 61 is a graphical depiction of the decreased TLR9-induced IFNα production in an ex vivo Whole Blood Assay from cynomolgus monkeys treated subcutaneously with BIIB059. Whole blood from cynomolgus monkeys treated with a single subcutaneous dose of 0.2 mg/kg BIIB059 was diluted 1:4 with complete RPMI 1640 and stimulated with CPG-A (2216) to a final concentration of 200 μg/ml in a 96 well round bottom tissue culture plate and incubated at 37° C. 5% CO2 for 18-20 hours. At the end of the culture, the stimulated whole blood was centrifuged to harvest serum. A549 cells were stimulated with the harvested serum for 19-20 hours at 37° C. 5% CO2 to induce MxA protein. After 20 hours, A549 cells were lysed and a sandwich ELISA was performed to detect concentrations of MxA protein. IFNα levels (units/mL) were back calculated from a standard curve generated by treating A549 cells with increasing doses of rIFNα. A one-way analysis of variance (ANOVA) with random effects was fit to log 10 values of the calculated concentrations of IFNα. IFNα values are plotted (on log 10 scale) versus day of bleed for each animal. Vertical lines denote groupings of bleed days into pre-dose, post-dose up to 33 days, and post-dose greater than 33-days. Bleed days later than day 33 were not used in the analysis. The model-based estimates of geometric mean IFNα values are represented by thick black horizontal lines within the pre- and post-dose regions of each panel. Graph and statistical analysis were calculated using the R language for statistical computing.



DETAILED DESCRIPTION
BIIB059 is an exemplary monoclonal antibody that specifically binds to human BDCA2. The anti-BDCA2 antibodies described herein inhibit pDC production and/or secretion of inflammatory cytokines and chemokines. Furthermore, anti-BDCA2 antibodies described herein can downregulate levels of CD32a and/or CD62L on the surface of pDCs. Also, the anti-BDCA2 antibodies of this disclosure can mediate internalization of BDCA2 from the surface of pDCs. In addition, the anti-BDCA2 antibodies described herein can be used to deplete pDCs by ADCC or CDC and can be used to treat or prevent immunological disorders such as inflammatory and autoimmune conditions. This disclosure also shows that combining an antimalarial with an anti-BDCA2 antibody described herein can yield improved effects compared to treatment with either agent alone.
BDCA2
BDCA2 is a type II C-type lectin that is specifically expressed on pDCs. BDCA2 consists of a single extracellular carbohydrate recognition domain (CRD) at its C-terminus, a transmembrane region, and a short cytoplasmic tail at its N-terminus that does not harbor a signaling motif. BDCA2 transmits intracellular signals through an associated transmembrane adaptor, FcεRIγ (see FIG. 1). Antibody-mediated ligation of BDCA2 leads to recruitment of spleen tyrosine kinase (SYK) to phosphorylated immunoreceptor tyrosine-based activation motif (ITAM) of FcεRIγ. Syk activation leads to the activation of B cell linker (Blnk), Bruton's tyrosine kinase (BTK), and phospholipase Cγ2 (PLCγ2), leading to Ca2+ mobilization.
The amino acid sequence of the human BDCA2 protein (GenBank® Accession No. NP_569708.1) is shown below (the transmembrane domain is italicized; the ectodomain is underlined).







(SEQ ID NO: 1)


  1 MVPEEEPQDR EKGLWWFQLK VWSMAVVSIL LLSVCFTVSS


 


    VVPHNFMYSK


 


 51 TVKRLSKLRE YQQYHPSLTC VMEGKDIEDW SCCPTPWTSF


 


    QSSCYFISTG


 


101 MQSWTKSQKN CSVMGADLVV INTREEQDFI IQNLKRNSSY


 


    FLGLSDPGGR


 


151 RHWQWVDQTP YNENVTFWHS GEPNNLDERC AIINFRSSEE


 


    WGWNDIHCHV


 


201 PQKSICKMKK IYI*






The amino acid sequence of the human FcεRIγ (GenBank® Accession No. NP_004097.1) is shown below.









(SEQ ID NO: 2)



 1 MIPAVVLLLL LLVEQAAALG EPQLCYILDA ILFLYGIVLT


 



   LLYCRLKIQV


 



51 RKAAITSYEK SDGVYTGLST RNQETYETLK HEKPPQ*






The closest rat BDCA2 homolog, rat Clec4b2 (GenBank® Accession No. NM_001005896), shares only 51.0% identity with human BDCA2. In contrast, the cynomolgus and rhesus monkey BDCA2 share 90.6% identity with human BDCA2. In addition, cynomolgus and rhesus monkey FcεRIγ protein sequence, which are identical to each other, shares 98.9% identity with human FcεRIγ protein.
The human, cynomolgus, and rhesus monkey BDCA2 proteins can be used as immunogens to prepare anti-BDCA2 antibodies. To prepare human anti-BDCA2 antibodies, the human BDCA2 protein can be used as the immunogen. Anti-human BDCA2 antibodies can then be screened to identify antibodies having one or more of the features described herein (e.g., reducing production/secretion of one or more of type I or type III interferons, IL-6, TNF-α, MIP-1-α, MIP-1β, CCL5, and IP-10/CXCL10; depleting pDCs; competing for binding to the extracellular domain of BDCA2 with BIIB059; selectively binding the ectodomain of human, cynomolgus and rhesus BDCA2 but not binding rat Clec4b2; inhibition of disease development in a human psoriatic xenograft model).
Anti-BDCA2 Antibodies
This disclosure includes the sequences of a monoclonal antibody, BIIB059, which binds to human, cynomolgus, and rhesus BDCA2, but not to rat Clec4b2. BIIB059 does not bind to or does not show significant binding to BDCA2 from phylogenetic species below primates.
BIIB059
BIIB059 is a humanized IgG1 antibody that specifically recognizes BDCA2 on the surface of plasmacytoid dendritic cells. It was derived from a murine antibody (24F4) that binds BDCA2 as follows. A plasmid encoding full-length human BDCA2 was injected into mice with a gene gun. Splenocytes from this mouse were fused to myeloma cells and the resulting hybridoma produced the 24F4 antibody. The 24F4 antibody was engineered into a wild-type human IgG1 framework to maintain full effector function. The predicted amino acid sequences of the mature BIIB059 heavy and light chains are shown below. Complementarity-determining regions (CDRs) 1, 2, and 3 of the variable light chain (VL) and the variable heavy chain (VH) are shown in that order from N to the C-terminus of the mature VL and VH sequences and are both underlined and boldened. An antibody consisting of the mature heavy chain (SEQ ID NO: 4) and the mature light chain (SEQ ID NO: 3) listed below is termed BIIB059.
Mature BIIB059 Light Chain (LC)









(SEQ ID NO: 3)



DIQLTQSPSS LSASVGDRVT ITCKASQSVD YDGDSYMNWY


 



QQKPGKAPKL LIYAASTLES GVPSRFSGSG SGTDFTLTIS


 



SLQPEDFATY YCQQANEDPR TFGQGTKVEI KRTVAAPSVF


 



IFPPSDEQLK SGTASVVCLL NNFYPREAKV QWKVDNALQS


 



GNSQESVTEQ DSKDSTYSLS STLTLSKADY EKHKVYACEV


 



THQGLSSPVT KSFNRGEC






Mature BIIB059 Heavy Chain (HC)










(SEQ ID NO: 4)



DVQLVESGGG LVKPGGSLRL SCAAS  TYTMSWVRQA


 



PGKGLEWVAT ISPGDSFGYY YPDSVQGRFT ISRDNAKNSL


 



YLQMNSLRAE DTAVYYCTRD IYYNYGAWFA YWGQGTLVTV


 



SSASTKGPSV FPLAPSSKST SGGTAALGCL VKDYFPEPVT


 



VSWNSGALTS GVHTFPAVLQ SSGLYSLSSV VTVPSSSLGT


 



QTYICNVNHK PSNTKVDKKV EPKSCDKTHT CPPCPAPELL


 



GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF


 



NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN


 



GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR


 



DELTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP


 



PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH


 



YTQKSLSLSP G






The variable light chain (VL) of BIIB059 has the following amino acid sequence:










(SEQ ID NO: 23)



DIQLTQSPSS LSASVGDRVT ITCKASQSVD YDGDSYMNWY


 



QQKPGKAPKL LIYAASTLES GVPSRFSGSG SGTDFTLTIS


 



SLQPEDFATY YCQQANEDPR TFGQGTKVEI K






The variable heavy chain (VH) of BIIB059 has the following amino acid sequence:










(SEQ ID NO: 24)



DVQLVESGGG LVKPGGSLRL SCAAS  TYTMSWVRQA


 



PGKGLEWVAT ISPGDSFGYY YPDSVQGRFT ISRDNAKNSL


 



YLQMNSLRAE DTAVYYCTRD IYYNYGAWFA YWGQGTLVTV SS






The amino acid sequences of VL CDRs of BII059 are listed below:










(SEQ ID NO: 5)



VL CDR1: KASQSVDYDGDSYMN;


 



(SEQ ID NO: 6)



VL CDR2: AASTLES;



and


 



(SEQ ID NO: 7)



VL CDR3: QQANEDPRT.






The amino acid sequences of the VH CDRs of BII059 are listed below:










(SEQ ID NO: 8)



VH CDR1: TYTMS (Kabat CDR1) or


 



(SEQ ID NO: 9)



GFTFSTYTMS (enhanced Chothia/AbM CDR1);


 



(SEQ ID NO: 10)



VH CDR2: TISPGDSFGYYYPDSVQG;


 



(SEQ ID NO: 11)



VH CDR3: DIYYNYGAWFAY






As indicated above, the enhanced Chothia/AbM CDR definition of the VH CDR1 is 5 amino acids longer than the Kabat definition of this CDR. The five additional amino acids of the enhanced Chothia/AbM VH CDR1 are GFTFS (SEQ ID NO:12).
The anti-BDCA2 antibodies of this disclosure can also comprise “alternate CDRs” of BIIB059. By “alternate” CDRs are meant CDRs (CDR1, CDR2, and CDR3) defined according to any one of the Chothia, from Abysis, enhanced Chothia/AbM CDR, or the contact definitions. These alternate CDRs can be obtained, e.g., by using the AbYsis database (www.bioinf.org.uk/abysis/sequence_input/key_annotation/key_annotation.cgi). The amino acid sequences of “alternate” CDRs 1, 2, and 3 of the heavy chain variable region and the light chain variable region of BIIB059 are compared with the CDRs defined according to Kabat in the Table below.
















Chothia,
Enhanced



Domain
Kabat
from Abysis
Chothia/AbM
Contact







VH CDR1
TYTMS
GFTFSTY
GFTFSTYTMS
STYTMS



(SEQ ID NO: 8)
(SEQ ID NO: 89)
(SEQ ID NO: 9)
(SEQ ID NO: 90)


 


VH CDR2
TISPGDSFGYYYPDSVQG
SPGDSFG
TISPGDSFGYY
WVATISPGDSFGYY



(SEQ ID NO: 10)
(SEQ ID NO: 91)
(SEQ ID NO: 92)
(SEQ ID NO: 93)


 


VH CDR3
DIYYNYGAWFAY
DIYYNYGAWFAY
DIYYNYGAWFAY
TRDIYYNYGAWFA



(SEQ ID NO: 11)
(SEQ ID NO: 11)
(SEQ ID NO: 11)
(SEQ ID NO: 94)


 


VL CDR1
KASQSVDYDGDSYMN
KASQSVDYDGDSYMN
KASQSVDYDGDSYMN
DYDGDSYMNWY



(SEQ ID NO: 5)
(SEQ ID NO: 5)
(SEQ ID NO: 5)
(SEQ ID NO: 95)


 


VL CDR2
AASTLES
AASTLES
AASTLES
LLIYAASTLE



(SEQ ID NO: 6)
(SEQ ID NO: 6)
(SEQ ID NO: 6)
(SEQ ID NO: 96)


 


VL CDR3
QQANEDPRT
QQANEDPRT
QQANEDPRT
QQANEDPR



(SEQ ID NO: 7)
(SEQ ID NO: 7)
(SEQ ID NO: 7)
(SEQ ID NO: 97)









The anti-BDCA2 antibodies can encompass the heavy chain and light chain CDR 1, CDR2, and CDR3 according to the Kabat definition, the Chothia from Abysis definition, the enhanced Chothia/AbM CDR definition, or the contact definition. These antibodies can have, e.g., 1, 2 or 3 substitutions within one or more (i.e., 1, 2, 3, 4, 5 or 6) of the CDRs. These antibodies (i) bind human or cynomolgus monkey BDCA2 but do not significantly bind BDCA2 from phylogenetic species below primates; and/or (ii) inhibit TLR7/TLR9-induced type I interferon and other cytokine or chemokine production by human pDCs; and/or (iii) mediate internalization of BDCA2 from the surface of pDCs; and/or (iv) downregulate CD32a and/or CD62L from the surface of pDCs; and/or (v) deplete pDCs in vitro by ADCC or CDC.
Human IgG antibodies are tetrameric molecules containing two light chains and two heavy chains. Each light chain of BIIB059 is covalently linked to a heavy chain through an interchain disulfide bond (LC Cys 218-HC Cys 225) and the heavy chains are paired to each other by two interchain disulfides (HC Cys 231-Cys 231 and Cys 234-Cys 234). All other cysteines form intramolecular disulfides that stabilize the constant and variable domains.
In certain embodiments, the anti-BDCA2 antibodies include a human heavy chain and light chain constant region. In certain embodiments, the heavy chain constant region comprises a CH1 domain and a hinge region. In some embodiments, the heavy chain constant region comprises a CH3 domain. If the heavy chain constant region includes substitutions, such substitutions modify the properties of the antibody (e.g., increase or decrease one or more of: Fc receptor binding, antibody glycosylation, the number of cysteine residues, effector cell function, or complement function). In certain embodiments, the antibody is an IgG antibody. In specific embodiments, the antibody is selected from the group consisting of IgG1, IgG2, IgG3, and IgG4. In certain embodiments, the antibody includes a human Fc region that binds FcγRIIa (CD32a) with an affinity of 7 μg/mL to 15 μg/mL. In certain embodiments, the antibody includes a human Fc region that binds FcγRII (CD32a) with an EC50 of 10 μg/mL. In certain embodiments, the antibody includes a human Fc region that binds FcγRIIa (CD32a) with an EC50 of 11 μg/mL. In certain embodiments, the antibody includes a human Fc region that binds FcγRIIa (CD32a) with an EC50 of 12 μg/mL. Table 1 provides a list of the properties of the BIIB059 antibody.








TABLE 1







Molecular Mass (estimated/
146,348.2 Da/146,352 Da


deglycosylated actual)


Molecular Mass (deglycosylated
49,425.8 Da/49,424 Da


heavy chain, expected/actual)


Molecular Mass
23,764.3 Da/23,765 Da


(light chain, expected/actual)


Molecular Mass (SDS-PAGE)
150,000 Da


Extinction Coefficient (1 mg/mL)
1.46 mL/mg/cm at 280 nm


Absorbance Maximum
275 nm


pI (calculated)
7.26


pI (IEF)
Major component 7.01



Minor components 6.90, 6.81, 6.78,



7.09


EC50 human BDCA2 (FACS)
7 nM


EC50 cyno BDCA2 (FACS)
7 nM


Tm by DSC:
CH2: 72° C.



Fab: 68.6° C., 75.9° C.



CH3: 85° C.


Free SH
0.4/mole (1.1%)


Glycation
0.1 mole/mole BIIB059


N-linked glycosylation RRS2
G0 (69.2%)



G1 (23.9%)



G2 (2.2%)



Aglycosylated (1%)


Exemplary Formulation Buffer
20 mM sodium citrate, 150 mM



NaCl pH 6.0


Solubility in formulation buffer
>150 mg/mL


Aggregation (SEC)
0.2%


Aggregation (AUC)
0.3% (primarily dimers)


T1/2
7.3 days in rats


Endotoxin
<0.05 EU/mg protein









BIIB059 exhibits suitable physicochemical properties for an antibody therapeutic. This antibody shows low levels of aggregation. The wild-type IgG1 framework contains a single N-linked glycosylation site in the molecule and BIIB059 and binds to Fc receptors with affinities typical of this class of molecules. The calculated pI of 7.26 is somewhat low for an antibody. Charge heterogeneity detected in BIIB059 suggests that a significant fraction of BIIB059 contains modifications. Glycation levels of up to about 10% detected in purified batches of BIIB059 account at least in part for this charge heterogeneity. The folding Tm for the BIIB059 is at the lower end of typical values observed for antibodies, while those for the CH2 and CH3 domains are typical for a fully glycosylated IgG1 mAb. Based on differential scanning fluorimetry and viscosity measurements the BIIB059 can be formulated, e.g., at 50 mg/mL in 20 mM sodium citrate, 150 mM NaCl, pH 6.0. This antibody can also be formulated at much higher concentrations, such as 150-300 mg/mL (e.g., 150 mg/mL, 200 mg/mL, 250 mg/mL, 300 mg/mL).
BIIB059 is a fully humanized, Fc function-competent IgG1 mAb that exhibits high affinity for BDCA2 and binds equally well to native human and cynomolgus BDCA2. BIIB059 is a potent inhibitor of all TLR9-induced type I IFNs as well as other cytokines and chemokines by pDCs. BIIB059 is equally potent at inhibiting TLR9-induced type I interferon by pDCs from healthy human donors and SLE patients. BIIB059 specifically inhibits TLR9-induced type I IFN by pDCs and does not impact IFN production by other cell types triggered with different TLR ligand. BIIB059 leads to rapid internalization of BDCA2 from the cell surface. Upon stimulation, BDCA2 colocalize with TLR9 in the endosomal/lysosomal compartment which appears to be necessary for its inhibition of TLR9 signaling. BIIB059 was found to cause CD62L shedding from the surface of human pDCs which might impact their homing to target organs. In vitro antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) studies suggest that BIIB059 may have cell depletion activity in cell lines overexpressing BDCA2. However, the fact that BIIB059 leads to rapid and complete internalization of BDCA2 from the surface of pDCs makes it less likely that BIIB059 would effect sustained depletion pDCs in vivo. Combination of BIIB059 and hydroxychloroquine (HCQ) led to an additive inhibitory effect on TLR7 and TLR9-induced IFNα production by PBMC from healthy human donors. These data highlight the potential additive therapeutic benefit of BIIB059 when administered with antimalarial compounds such as HCQ.
Antibodies, such as BIIB059, can be made, for example, by preparing and expressing synthetic genes that encode the recited amino acid sequences or by mutating human germline genes to provide a gene that encodes the recited amino acid sequences. Moreover, this antibody and other anti-BDCA2 antibodies can be obtained, e.g., using one or more of the following methods.
Methods of Obtaining Anti-BDCA2 Antibodies
Numerous methods are available for obtaining antibodies, particularly human antibodies. One exemplary method includes screening protein expression libraries, e.g., phage or ribosome display libraries. Phage display is described, for example, in U.S. Pat. No. 5,223,409; Smith, Science 228:1315-1317 (1985); WO 92/18619; WO 91/17271; WO 92/20791; WO 92/15679; WO 93/01288; WO 92/01047; WO 92/09690; and WO 90/02809. The display of Fab's on phage is described, e.g., in U.S. Pat. Nos. 5,658,727; 5,667,988; and 5,885,793.
In addition to the use of display libraries, other methods can be used to obtain a BDCA2-binding antibody. For example, the BDCA2 protein or a peptide thereof can be used as an antigen in a non-human animal, e.g., a rodent, e.g., a mouse, hamster, or rat. In addition, cells transfected with a cDNA encoding BDCA2 can be injected into a non-human animal as a means of producing antibodies that effectively bind the cell surface BDCA2 protein.
In one embodiment, the non-human animal includes at least a part of a human immunoglobulin gene. For example, it is possible to engineer mouse strains deficient in mouse antibody production with large fragments of the human Ig loci. Using the hybridoma technology, antigen-specific monoclonal antibodies derived from the genes with the desired specificity may be produced and selected. See, e.g., XENOMOUSETM, Green et al., Nature Genetics 7:13-21 (1994), U.S. 2003-0070185, WO 96/34096, and WO 96/33735.
In another embodiment, a monoclonal antibody is obtained from the non-human animal, and then modified, e.g., humanized or deimmunized. Winter describes an exemplary CDR-grafting method that may be used to prepare humanized antibodies described herein (U.S. Pat. No. 5,225,539). All or some of the CDRs of a particular human antibody may be replaced with at least a portion of a non-human antibody. It may only be necessary to replace the CDRs required for binding or binding determinants of such CDRs to arrive at a useful humanized antibody that binds to BDCA2.
Humanized antibodies can be generated by replacing sequences of the Fv variable region that are not directly involved in antigen binding with equivalent sequences from human Fv variable regions. General methods for generating humanized antibodies are provided by Morrison, S. L., Science, 229:1202-1207 (1985), by Oi et al., BioTechniques, 4:214 (1986), and by U.S. Pat. No. 5,585,089; U.S. Pat. No. 5,693,761; U.S. Pat. No. 5,693,762; U.S. Pat. No. 5,859,205; and U.S. Pat. No. 6,407,213. Those methods include isolating, manipulating, and expressing the nucleic acid sequences that encode all or part of immunoglobulin Fv variable regions from at least one of a heavy or light chain. Sources of such nucleic acid are well known to those skilled in the art and, for example, may be obtained from a hybridoma producing an antibody against a predetermined target, as described above, from germline immunoglobulin genes, or from synthetic constructs. The recombinant DNA encoding the humanized antibody can then be cloned into an appropriate expression vector.
Human germline sequences, for example, are disclosed in Tomlinson, I. A. et al., J. Mol. Biol., 227:776-798 (1992); Cook, G. P. et al., Immunol. Today, 16: 237-242 (1995); Chothia, D. et al., J. Mol. Bio. 227:799-817 (1992); and Tomlinson et al., EMBO J., 14:4628-4638 (1995). The V BASE directory provides a comprehensive directory of human immunoglobulin variable region sequences (compiled by Tomlinson, I. A. et al. MRC Centre for Protein Engineering, Cambridge, UK). These sequences can be used as a source of human sequence, e.g., for framework regions and CDRs. Consensus human framework regions can also be used, e.g., as described in U.S. Pat. No. 6,300,064.
A non-human BDCA2-binding antibody may also be modified by specific deletion of human T cell epitopes or “deimmunization” by the methods disclosed in WO 98/52976 and WO 00/34317. Briefly, the heavy and light chain variable regions of an antibody can be analyzed for peptides that bind to MHC Class II; these peptides represent potential T-cell epitopes (as defined in WO 98/52976 and WO 00/34317). For detection of potential T-cell epitopes, a computer modeling approach termed “peptide threading” can be applied, and in addition a database of human MHC class II binding peptides can be searched for motifs present in the VH and VL sequences, as described in WO 98/52976 and WO 00/34317. These motifs bind to any of the 18 major MHC class II DR allotypes, and thus constitute potential T cell epitopes. Potential T-cell epitopes detected can be eliminated by substituting small numbers of amino acid residues in the variable regions, or preferably, by single amino acid substitutions. As far as possible, conservative substitutions are made. Often, but not exclusively, an amino acid common to a position in human germline antibody sequences may be used. After the deimmunizing changes are identified, nucleic acids encoding VH and VL can be constructed by mutagenesis or other synthetic methods (e.g., de novo synthesis, cassette replacement, and so forth). A mutagenized variable sequence can, optionally, be fused to a human constant region, e.g., human IgG1 or kappa constant regions.
In some cases, a potential T cell epitope will include residues known or predicted to be important for antibody function. For example, potential T cell epitopes are usually biased towards the CDRs. In addition, potential T cell epitopes can occur in framework residues important for antibody structure and binding. Changes to eliminate these potential epitopes will in some cases require more scrutiny, e.g., by making and testing chains with and without the change. Where possible, potential T cell epitopes that overlap the CDRs can be eliminated by substitutions outside the CDRs. In some cases, an alteration within a CDR is the only option, and thus variants with and without this substitution can be tested. In other cases, the substitution required to remove a potential T cell epitope is at a residue position within the framework that might be critical for antibody binding. In these cases, variants with and without this substitution are tested. Thus, in some cases several variant deimmunized heavy and light chain variable regions are designed and various heavy/light chain combinations are tested to identify the optimal deimmunized antibody. The choice of the final deimmunized antibody can then be made by considering the binding affinity of the different variants in conjunction with the extent of deimmunization, particularly, the number of potential T cell epitopes remaining in the variable region. Deimmunization can be used to modify any antibody, e.g., an antibody that includes a non-human sequence, e.g., a synthetic antibody, a murine antibody other non-human monoclonal antibody, or an antibody isolated from a display library.
Other methods for humanizing antibodies can also be used. For example, other methods can account for the three dimensional structure of the antibody, framework positions that are in three dimensional proximity to binding determinants, and immunogenic peptide sequences. See, e.g., WO 90/07861; U.S. Pat. Nos. 5,693,762; 5,693,761; 5,585,089; 5,530,101; and 6,407,213; Tempest et al. (1991) Biotechnology 9:266-271. Still another method is termed “humaneering” and is described, for example, in U.S. 2005-008625.
The antibody can include a human Fc region, e.g., a wild-type Fc region or an Fc region that includes one or more alterations. In one embodiment, the constant region is altered, e.g., mutated, to modify the properties of the antibody (e.g., to increase or decrease one or more of: Fc receptor binding, antibody glycosylation, the number of cysteine residues, effector cell function, or complement function). For example, the human IgG1 constant region can be mutated at one or more residues, e.g., one or more of residues 234 and 237 (based on Kabat numbering). Antibodies may have mutations in the CH2 region of the heavy chain that reduce or alter effector function, e.g., Fc receptor binding and complement activation. For example, antibodies may have mutations such as those described in U.S. Pat. Nos. 5,624,821 and 5,648,260. Antibodies may also have mutations that stabilize the disulfide bond between the two heavy chains of an immunoglobulin, such as mutations in the hinge region of IgG4, as disclosed in the art (e.g., Angal et al. (1993) Mol. Immunol. 30:105-08). See also, e.g., U.S. 2005-0037000.
Affinity Maturation
In one embodiment, an anti-BDCA2 antibody or antigen-binding fragment thereof is modified, e.g., by mutagenesis, to provide a pool of modified antibodies. The modified antibodies are then evaluated to identify one or more antibodies having altered functional properties (e.g., improved binding, improved stability, reduced antigenicity, or increased stability in vivo). In one implementation, display library technology is used to select or screen the pool of modified antibodies. Higher affinity antibodies are then identified from the second library, e.g., by using higher stringency or more competitive binding and washing conditions. Other screening techniques can also be used.
In some implementations, the mutagenesis is targeted to regions known or likely to be at the binding interface. If, for example, the identified binding proteins are antibodies, then mutagenesis can be directed to the CDR regions of the heavy or light chains as described herein. Further, mutagenesis can be directed to framework regions near or adjacent to the CDRs, e.g., framework regions, particularly within 10, 5, or 3 amino acids of a CDR junction. In the case of antibodies, mutagenesis can also be limited to one or a few of the CDRs, e.g., to make step-wise improvements.
In one embodiment, mutagenesis is used to make an antibody more similar to one or more germline sequences. One exemplary germlining method can include: identifying one or more germline sequences that are similar (e.g., most similar in a particular database) to the sequence of the isolated antibody. Then mutations (at the amino acid level) can be made in the isolated antibody, either incrementally, in combination, or both. For example, a nucleic acid library that includes sequences encoding some or all possible germline mutations is made. The mutated antibodies are then evaluated, e.g., to identify an antibody that has one or more additional germline residues relative to the isolated antibody and that is still useful (e.g., has a functional activity). In one embodiment, as many germline residues are introduced into an isolated antibody as possible.
In one embodiment, mutagenesis is used to substitute or insert one or more germline residues into a CDR region. For example, the germline CDR residue can be from a germline sequence that is similar (e.g., most similar) to the variable region being modified. After mutagenesis, activity (e.g., binding or other functional activity) of the antibody can be evaluated to determine if the germline residue or residues are tolerated. Similar mutagenesis can be performed in the framework regions.
Selecting a germline sequence can be performed in different ways. For example, a germline sequence can be selected if it meets a predetermined criteria for selectivity or similarity, e.g., at least a certain percentage identity, e.g., at least 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, or 99.5% identity, relative to the donor non-human antibody. The selection can be performed using at least 2, 3, 5, or 10 germline sequences. In the case of CDR1 and CDR2, identifying a similar germline sequence can include selecting one such sequence. In the case of CDR3, identifying a similar germline sequence can include selecting one such sequence, but may include using two germline sequences that separately contribute to the amino-terminal portion and the carboxy-terminal portion. In other implementations, more than one or two germline sequences are used, e.g., to form a consensus sequence.
Calculations of “sequence identity” between two sequences are performed as follows. The sequences are aligned for optimal comparison purposes (e.g., gaps can be introduced in one or both of a first and a second amino acid or nucleic acid sequence for optimal alignment and non-homologous sequences can be disregarded for comparison purposes). The optimal alignment is determined as the best score using the GAP program in the GCG software package with a Blossum 62 scoring matrix with a gap penalty of 12, a gap extend penalty of 4, and a frameshift gap penalty of 5. The amino acid residues or nucleotides at corresponding amino acid positions or nucleotide positions are then compared. When a position in the first sequence is occupied by the same amino acid residue or nucleotide as the corresponding position in the second sequence, then the molecules are identical at that position. The percent identity between the two sequences is a function of the number of identical positions shared by the sequences.
In other embodiments, the antibody may be modified to have an altered glycosylation pattern (i.e., altered from the original or native glycosylation pattern). As used in this context, “altered” means having one or more carbohydrate moieties deleted, and/or having one or more glycosylation sites added to the original antibody. Addition of glycosylation sites to the presently disclosed antibodies may be accomplished by altering the amino acid sequence to contain glycosylation site consensus sequences; such techniques are well known in the art. Another means of increasing the number of carbohydrate moieties on the antibodies is by chemical or enzymatic coupling of glycosides to the amino acid residues of the antibody. These methods are described in, e.g., WO 87/05330, and Aplin and Wriston (1981) CRC Crit. Rev. Biochem., 22:259-306. Removal of any carbohydrate moieties present on the antibodies may be accomplished chemically or enzymatically as described in the art (Hakimuddin et al. (1987) Arch. Biochem. Biophys., 259:52; Edge et al. (1981) Anal. Biochem., 118:131; and Thotakura et al. (1987) Meth. Enzymol., 138:350). See, e.g., U.S. Pat. No. 5,869,046 for a modification that increases in vivo half life by providing a salvage receptor binding epitope.
In one embodiment, an antibody has CDR sequences (e.g., a Chothia or Kabat CDR) that differ from those of the BIIB059 monoclonal antibody. CDR sequences that differ from those of the BIIB059 monoclonal antibody include amino acid changes, such as substitutions of 1, 2, 3, or 4 amino acids if a CDR is 5-7 amino acids in length, or substitutions of 1, 2, 3, 4, 5, 6, or 7 of amino acids in the sequence of a CDR if a CDR is 10 amino acids or greater in length. The amino acid that is substituted can have similar charge, hydrophobicity, or stereochemical characteristics. In some embodiments, the amino acid substitution(s) is a conservative substitution. In other embodiments, the amino acid substitution(s) is a non-conservative substitution. Such substitutions are within the ordinary skill of an artisan. The antibody or antibody fragments thereof that contain the substituted CDRs can be screened to identify antibodies having one or more of the features described herein (e.g., reducing production/secretion of type I or type III interferons, IL-6, TNF-α, MIP-1-α/CCL3, MIP-1β/CCL4, CCL5/RANTES, IP-10/CXCL10; depleting pDCs; competing for binding to the extracellular domain of BDCA2 with BIIB059; selectively binding the ectodomain of human, cynomolgus and rhesus BDCA2 but not binding rat Clec4b2 or binding to rat Clec4b2 with a lower binding affinity than to human, cynomolgus or rhesus BDCA2; inhibition of disease development in a human psoriatic xenograft model).
Unlike in CDRs, more substantial changes in structure framework regions (FRs) can be made without adversely affecting the binding properties of an antibody. Changes to FRs include, but are not limited to, humanizing a nonhuman-derived framework or engineering certain framework residues that are important for antigen contact or for stabilizing the binding site, e.g., changing the class or subclass of the constant region, changing specific amino acid residues which might alter an effector function such as Fc receptor binding (Lund et al., J. Immun., 147:2657-62 (1991); Morgan et al., Immunology, 86:319-24 (1995)), or changing the species from which the constant region is derived.
The anti-BDCA2 antibodies can be in the form of full length antibodies, or in the form of low molecular weight forms (e.g., biologically active antibody fragments or minibodies) of the anti-BDCA2 antibodies, e.g., Fab, Fab′, F(ab′)2, Fv, Fd, dAb, scFv, and sc(Fv)2. Other anti-BDCA2 antibodies encompassed by this disclosure include single domain antibody (sdAb) containing a single variable chain such as, VH or VL, or a biologically active fragment thereof. See, e.g., Moller et al., J. Biol. Chem., 285(49): 38348-38361 (2010); Harmsen et al., Appl. Microbiol. Biotechnol., 77(1):13-22 (2007); U.S. 2005/0079574 and Davies et al. (1996) Protein Eng., 9(6):531-7. Like a whole antibody, a sdAb is able to bind selectively to a specific antigen. With a molecular weight of only 12-15 kDa, sdAbs are much smaller than common antibodies and even smaller than Fab fragments and single-chain variable fragments.
Provided herein are compositions comprising a mixture of an anti-BDCA2 antibody or antigen-binding fragment thereof and one or more acidic variants thereof, e.g., wherein the amount of acidic variant(s) is less than about 80%, 70%, 60%, 60%, 50%, 40%, 30%, 30%, 20%, 10%, 5% or 1%. Also provided are compositions comprising an anti-BDCA2 antibody or antigen-binding fragment thereof comprising at least one deamidation site, wherein the pH of the composition is from about 5.0 to about 6.5, such that, e.g., at least about 90% of the anti-BDCA2 antibodies are not deamidated (i.e., less than about 10% of the antibodies are deamidated). In certain embodiments, less than about 5%, 3%, 2% or 1% of the antibodies are deamidated. The pH may be from 5.0 to 6.0, such as 5.5 or 6.0. In certain embodiments, the pH of the composition is 5.5, 5.6, 5.7, 5.8, 5.9, 6.0, 6.1, 6.2, 6.3, 6.4 or 6.5.
An “acidic variant” is a variant of a polypeptide of interest which is more acidic (e.g. as determined by cation exchange chromatography) than the polypeptide of interest. An example of an acidic variant is a deamidated variant.
A “deamidated” variant of a polypeptide molecule is a polypeptide wherein one or more asparagine residue(s) of the original polypeptide have been converted to aspartate, i.e. the neutral amide side chain has been converted to a residue with an overall acidic character.
The term “mixture” as used herein in reference to a composition comprising an anti-BDCA2 antibody or antigen-binding fragment thereof, means the presence of both the desired anti-BDCA2 antibody or antigen-binding fragment thereof and one or more acidic variants thereof. The acidic variants may comprise predominantly deamidated anti-BDCA2 antibody, with minor amounts of other acidic variant(s).
In certain embodiments, the binding affinity (KD), on-rate (KD on) and/or off-rate (KD off) of the antibody that was mutated to eliminate deamidation is similar to that of the wild-type antibody, e.g., having a difference of less than about 5 fold, 2 fold, 1 fold (100%), 50%, 30%, 20%, 10%, 5%, 3%, 2% or 1%.
In certain embodiments, an anti-BDCA2 antibody or antigen-binding fragment thereof or low molecular weight antibodies thereof bind to BDCA2 on pDCs and inhibit or reduce the production and/or secretion by pDCs of type I and type III IFNs, IL-6, TNF-α, and other inflammatory cytokines and chemokines (e.g., MIP-1α/CCL3, MIP-1β/CCL4, CCL5, and IP-10/CXCL10); and/or depletes pDCs by ADCC or CDC or apoptosis; and/or reduces the severity of symptoms when administered to human patients having one or more of, or animal models of: systemic lupus erythematosus, cutaneous lupus, discoid lupus, lupus nephritis, scleroderma, morphea, rheumatoid arthritis, polymyositis-dermatomyositis, psoriasis, Sjogren's syndrome, vasculitis, and Type I diabetes. In one embodiment, the anti-BDCA2 antibody or antigen-binding fragment thereof or low molecular weight antibodies thereof inhibit disease development in a human psoriatic xenograft model (Nestle et al., J. Exp. Med., 202(1):135-143 (2005)). These features of an anti-BDCA2 antibody or antigen-binding fragment thereof or low molecular weight antibodies thereof can be measured according to the methods described in the Examples as well as by other methods known in the art.
Antibody Fragments
Antibody fragments (e.g., Fab, Fab′, F(ab′)2, Facb, and Fv) may be prepared by proteolytic digestion of intact antibodies. For example, antibody fragments can be obtained by treating the whole antibody with an enzyme such as papain, pepsin, or plasmin. Papain digestion of whole antibodies produces F(ab)2 or Fab fragments; pepsin digestion of whole antibodies yields F(ab′)2 or Fab′; and plasmin digestion of whole antibodies yields Facb fragments.
Alternatively, antibody fragments can be produced recombinantly. For example, nucleic acids encoding the antibody fragments of interest can be constructed, introduced into an expression vector, and expressed in suitable host cells. See, e.g., Co, M. S. et al., J. Immunol., 152:2968-2976 (1994); Better, M. and Horwitz, A. H., Methods in Enzymology, 178:476-496 (1989); Plueckthun, A. and Skerra, A., Methods in Enzymology, 178:476-496 (1989); Lamoyi, E., Methods in Enzymology, 121:652-663 (1989); Rousseaux, J. et al., Methods in Enzymology, (1989) 121:663-669 (1989); and Bird, R. E. et al., TIBTECH, 9:132-137 (1991)). Antibody fragments can be expressed in and secreted from E. coli, thus allowing the facile production of large amounts of these fragments. Antibody fragments can be isolated from the antibody phage libraries. Alternatively, Fab′-SH fragments can be directly recovered from E. coli and chemically coupled to form F(ab)2 fragments (Carter et al., Bio/Technology, 10:163-167 (1992)). According to another approach, F(ab′)2 fragments can be isolated directly from recombinant host cell culture. Fab and F(ab′) 2 fragment with increased in vivo half-life comprising a salvage receptor binding epitope residues are described in U.S. Pat. No. 5,869,046.
Minibodies
Minibodies of anti-BDCA2 antibodies include diabodies, single chain (scFv), and single-chain (Fv)2 (sc(Fv)2).
A “diabody” is a bivalent minibody constructed by gene fusion (see, e.g., Holliger, P. et al., Proc. Natl. Acad. Sci. U.S.A., 90:6444-6448 (1993); EP 404,097; WO 93/11161). Diabodies are dimers composed of two polypeptide chains. The VL and VH domain of each polypeptide chain of the diabody are bound by linkers. The number of amino acid residues that constitute a linker can be between 2 to 12 residues (e.g., 3-10 residues or five or about five residues). The linkers of the polypeptides in a diabody are typically too short to allow the VL and VH to bind to each other. Thus, the VL and VH encoded in the same polypeptide chain cannot form a single-chain variable region fragment, but instead form a dimer with a different single-chain variable region fragment. As a result, a diabody has two antigen-binding sites.\
An scFv is a single-chain polypeptide antibody obtained by linking the VH and VL with a linker (see e.g., Huston et al., Proc. Natl. Acad. Sci. U.S.A., 85:5879-5883 (1988); and Plickthun, “The Pharmacology of Monoclonal Antibodies” Vol. 113, Ed Resenburg and Moore, Springer Verlag, New York, pp. 269-315, (1994)). The order of VHs and VLs to be linked is not particularly limited, and they may be arranged in any order. Examples of arrangements include: [VH] linker [VL]; or [VL] linker [VH]. The H chain V region and L chain V region in an scFv may be derived from any anti-BDCA2 antibody or antigen-binding fragment thereof described herein.
An sc(Fv)2 is a minibody in which two VHs and two VLs are linked by a linker to form a single chain (Hudson, et al., J. Immunol. Methods, (1999) 231: 177-189 (1999)). An sc(Fv)2 can be prepared, for example, by connecting scFvs with a linker. The sc(Fv)2 of the present invention include antibodies preferably in which two VHs and two VLs are arranged in the order of: VH, VL, VH, and VL ([VH] linker [VL] linker [VH] linker [VL]), beginning from the N terminus of a single-chain polypeptide; however the order of the two VHs and two VLs is not limited to the above arrangement, and they may be arranged in any order. Examples of arrangements are listed below:
[VL] linker [VH] linker [VH] linker [VL]
[VH] linker [VL] linker [VL] linker [VH]
[VH] linker [VH] linker [VL] linker [VL]
[VL] linker [VL] linker [VH] linker [VH]
[VL] linker [VH] linker [VL] linker [VH]
Normally, three linkers are required when four antibody variable regions are linked; the linkers used may be identical or different. There is no particular limitation on the linkers that link the VH and VL regions of the minibodies. In some embodiments, the linker is a peptide linker. Any arbitrary single-chain peptide comprising about three to 25 residues (e.g., 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18) can be used as a linker. Examples of such peptide linkers include: Ser; Gly Ser; Gly Gly Ser; Ser Gly Gly; Gly Gly Gly Ser (SEQ ID NO:13); Ser Gly Gly Gly (SEQ ID NO:14); Gly Gly Gly Gly Ser (SEQ ID NO:15); Ser Gly Gly Gly Gly (SEQ ID NO: 16); Gly Gly Gly Gly Gly Ser (SEQ ID NO: 17); Ser Gly Gly Gly Gly Gly (SEQ ID NO: 18); Gly Gly Gly Gly Gly Gly Ser (SEQ ID NO: 19); Ser Gly Gly Gly Gly Gly Gly (SEQ ID NO: 20); (Gly Gly Gly Gly Ser (SEQ ID NO: 21)n, wherein n is an integer of one or more; and
(Ser Gly Gly Gly Gly (SEQ ID NO: 22)n, wherein n is an integer of one or more.
In certain embodiments, the linker is a synthetic compound linker (chemical cross-linking agent). Examples of cross-linking agents that are available on the market include N-hydroxysuccinimide (NHS), disuccinimidylsuberate (DSS), bis(sulfosuccinimidyl)suberate (BS3), dithiobis(succinimidylpropionate) (DSP), dithiobis(sulfosuccinimidylpropionate) (DTSSP), ethyleneglycol bis(succinimidylsuccinate) (EGS), ethyleneglycol bis(sulfosuccinimidylsuccinate) (sulfo-EGS), disuccinimidyl tartrate (DST), disulfosuccinimidyl tartrate (sulfo-DST), bis[2-(succinimidooxycarbonyloxy)ethyl]sulfone (BSOCOES), and bis[2-(sulfosuccinimidooxycarbonyloxy)ethyl]sulfone (sulfo-BSOCOES).
The amino acid sequence of the VH or VL in the minibodies may include modifications such as substitutions, deletions, additions, and/or insertions. For example, the modification may be in one or more of the CDRs of the anti-BDCA2 antibody or antigen-binding fragment thereof (e.g., BIIB059). In certain embodiments, the modification involves one, two, or three amino acid substitutions in one or more CDRs of the VH and/or VL domain of the anti-BDCA2 minibody. Such substitutions are made to improve the binding and/or functional activity of the anti-BDCA2 minibody. In other embodiments, one, two, or three amino acids of the CDRs of the anti-BDCA2 antibody or antigen-binding fragment thereof (e.g., BIIB059) may be deleted or added as long as there is BDCA2 binding and/or functional activity when VH and VL are associated.
Bispecific Antibodies
Bispecific antibodies are antibodies that have binding specificities for at least two different epitopes. Exemplary bispecific antibodies may bind to two different epitopes of the BDCA2 protein. Other such antibodies may combine a BDCA2 binding site with a binding site for another protein. Bispecific antibodies can be prepared as full length antibodies or low molecular weight forms thereof (e.g., F(ab′)2 bispecific antibodies, sc(Fv)2 bispecific antibodies, diabody bispecific antibodies).
Traditional production of full length bispecific antibodies is based on the co-expression of two immunoglobulin heavy chain-light chain pairs, where the two chains have different specificities (Millstein et al., Nature, 305:537-539 (1983)). In a different approach, antibody variable domains with the desired binding specificities are fused to immunoglobulin constant domain sequences. DNAs encoding the immunoglobulin heavy chain fusions and, if desired, the immunoglobulin light chain, are inserted into separate expression vectors, and are co-transfected into a suitable host cell. This provides for greater flexibility in adjusting the proportions of the three polypeptide fragments. It is, however, possible to insert the coding sequences for two or all three polypeptide chains into a single expression vector when the expression of at least two polypeptide chains in equal ratios results in high yields.
According to another approach described in U.S. Pat. No. 5,731,168, the interface between a pair of antibody molecules can be engineered to maximize the percentage of heterodimers that are recovered from recombinant cell culture. The preferred interface comprises at least a part of the CH3 domain. In this method, one or more small amino acid side chains from the interface of the first antibody molecule are replaced with larger side chains (e.g., tyrosine or tryptophan). Compensatory “cavities” of identical or similar size to the large side chain(s) are created on the interface of the second antibody molecule by replacing large amino acid side chains with smaller ones (e.g., alanine or threonine). This provides a mechanism for increasing the yield of the heterodimer over other unwanted end-products such as homodimers.
Bispecific antibodies include cross-linked or “heteroconjugate” antibodies. For example, one of the antibodies in the heteroconjugate can be coupled to avidin, the other to biotin. Heteroconjugate antibodies may be made using any convenient cross-linking methods.
The “diabody” technology provides an alternative mechanism for making bispecific antibody fragments. The fragments comprise a VH connected to a VL by a linker which is too short to allow pairing between the two domains on the same chain. Accordingly, the VH and VL domains of one fragment are forced to pair with the complementary VL and VH domains of another fragment, thereby forming two antigen-binding sites.
Multivalent Antibodies
A multivalent antibody may be internalized (and/or catabolized) faster than a bivalent antibody by a cell expressing an antigen to which the antibodies bind. The antibodies describe herein can be multivalent antibodies with three or more antigen binding sites (e.g., tetravalent antibodies), which can be readily produced by recombinant expression of nucleic acid encoding the polypeptide chains of the antibody. The multivalent antibody can comprise a dimerization domain and three or more antigen binding sites. An exemplary dimerization domain comprises (or consists of) an Fc region or a hinge region. A multivalent antibody can comprise (or consist of) three to about eight (e.g., four) antigen binding sites. The multivalent antibody optionally comprises at least one polypeptide chain (e.g., at least two polypeptide chains), wherein the polypeptide chain(s) comprise two or more variable domains. For instance, the polypeptide chain(s) may comprise VD1-(X1)n-VD2-(X2)n-Fc, wherein VD1 is a first variable domain, VD2 is a second variable domain, Fc is a polypeptide chain of an Fc region, X1 and X2 represent an amino acid or peptide spacer, and n is 0 or 1.
Conjugated Antibodies
The antibodies disclosed herein may be conjugated antibodies which are bound to various molecules including macromolecular substances such as polymers (e.g., polyethylene glycol (PEG), polyethylenimine (PEI) modified with PEG (PEI-PEG), polyglutamic acid (PGA) (N-(2-Hydroxypropyl) methacrylamide (HPMA) copolymers), hyaluronic acid, radioactive materials (e.g. 90Y, 131I) fluorescent substances, luminescent substances, haptens, enzymes, metal chelates, drugs, and toxins (e.g., calcheamicin, Pseudomonas exotoxin A, ricin (e.g. deglycosylated ricin A chain)).
In one embodiment, to improve the cytotoxic actions of anti-BDCA2 antibodies and consequently their therapeutic effectiveness, the antibodies are conjugated with highly toxic substances, including radioisotopes and cytotoxic agents. These conjugates can deliver a toxic load selectively to the target site (i.e., cells expressing the antigen recognized by the antibody) while cells that are not recognized by the antibody are spared. In order to minimize toxicity, conjugates are generally engineered based on molecules with a short serum half-life (thus, the use of murine sequences, and IgG3 or IgG4 isotypes).
In certain embodiments, an anti-BDCA2 antibody or antigen-binding fragment thereof are modified with a moiety that improves its stabilization and/or retention in circulation, e.g., in blood, serum, or other tissues, e.g., by at least 1.5, 2, 5, 10, or 50 fold. For example, the anti-BDCA2 antibody or antigen-binding fragment thereof can be associated with (e.g., conjugated to) a polymer, e.g., a substantially non-antigenic polymer, such as a polyalkylene oxide or a polyethylene oxide. Suitable polymers will vary substantially by weight. Polymers having molecular number average weights ranging from about 200 to about 35,000 Daltons (or about 1,000 to about 15,000, and 2,000 to about 12,500) can be used. For example, the anti-BDCA2 antibody or antigen-binding fragment thereof can be conjugated to a water soluble polymer, e.g., a hydrophilic polyvinyl polymer, e.g., polyvinylalcohol or polyvinylpyrrolidone. Examples of such polymers include polyalkylene oxide homopolymers such as polyethylene glycol (PEG) or polypropylene glycols, polyoxyethylenated polyols, copolymers thereof and block copolymers thereof, provided that the water solubility of the block copolymers is maintained. Additional useful polymers include polyoxyalkylenes such as polyoxyethylene, polyoxypropylene, and block copolymers of polyoxyethylene and polyoxypropylene; polymethacrylates; carbomers; and branched or unbranched polysaccharides.
The above-described conjugated antibodies can be prepared by performing chemical modifications on the antibodies or the lower molecular weight forms thereof described herein. Methods for modifying antibodies are well known in the art (e.g., U.S. Pat. No. 5,057,313 and U.S. Pat. No. 5,156,840).
Methods of Producing Antibodies
Antibodies may be produced in bacterial or eukaryotic cells. Some antibodies, e.g., Fab's, can be produced in bacterial cells, e.g., E. coli cells. Antibodies can also be produced in eukaryotic cells such as transformed cell lines (e.g., CHO, 293E, COS). In addition, antibodies (e.g., scFv's) can be expressed in a yeast cell such as Pichia (see, e.g., Powers et al., J Immunol Methods. 251:123-35 (2001)), Hanseula, or Saccharomyces. To produce the antibody of interest, a polynucleotide encoding the antibody is constructed, introduced into an expression vector, and then expressed in suitable host cells. Standard molecular biology techniques are used to prepare the recombinant expression vector, transfect the host cells, select for transformants, culture the host cells and recover the antibody.
If the antibody is to be expressed in bacterial cells (e.g., E. coli), the expression vector should have characteristics that permit amplification of the vector in the bacterial cells. Additionally, when E. coli such as JM109, DH5α, HB101, or XL1-Blue is used as a host, the vector must have a promoter, for example, a lacZ promoter (Ward et al., 341:544-546 (1989), araB promoter (Better et al., Science, 240:1041-1043 (1988)), or T7 promoter that can allow efficient expression in E. coli. Examples of such vectors include, for example, M13-series vectors, pUC-series vectors, pBR322, pBluescript, pCR-Script, pGEX-5X-1 (Pharmacia), “QIAexpress system” (QIAGEN), pEGFP, and pET (when this expression vector is used, the host is preferably BL21 expressing T7 RNA polymerase). The expression vector may contain a signal sequence for antibody secretion. For production into the periplasm of E. coli, the pelB signal sequence (Lei et al., J. Bacteriol., 169:4379 (1987)) may be used as the signal sequence for antibody secretion. For bacterial expression, calcium chloride methods or electroporation methods may be used to introduce the expression vector into the bacterial cell.
If the antibody is to be expressed in animal cells such as CHO, COS, and NIH3T3 cells, the expression vector includes a promoter necessary for expression in these cells, for example, an SV40 promoter (Mulligan et al., Nature, 277:108 (1979)), MMLV-LTR promoter, EF1α promoter (Mizushima et al., Nucleic Acids Res., 18:5322 (1990)), or CMV promoter. In addition to the nucleic acid sequence encoding the immunoglobulin or domain thereof, the recombinant expression vectors may carry additional sequences, such as sequences that regulate replication of the vector in host cells (e.g., origins of replication) and selectable marker genes. The selectable marker gene facilitates selection of host cells into which the vector has been introduced (see e.g., U.S. Pat. Nos. 4,399,216, 4,634,665 and 5,179,017). For example, typically the selectable marker gene confers resistance to drugs, such as G418, hygromycin, or methotrexate, on a host cell into which the vector has been introduced. Examples of vectors with selectable markers include pMAM, pDR2, pBK-RSV, pBK-CMV, pOPRSV, and pOP13.
In one embodiment, antibodies are produced in mammalian cells. Exemplary mammalian host cells for expressing an antibody include Chinese Hamster Ovary (CHO cells) (including dhfr− CHO cells, described in Urlaub and Chasin (1980) Proc. Natl. Acad. Sci. USA 77:4216-4220, used with a DHFR selectable marker, e.g., as described in Kaufman and Sharp (1982) Mol. Biol. 159:601-621), human embryonic kidney 293 cells (e.g., 293, 293E, 293T), COS cells, NIH3T3 cells, lymphocytic cell lines, e.g., NS0 myeloma cells and SP2 cells, and a cell from a transgenic animal, e.g., a transgenic mammal. For example, the cell is a mammary epithelial cell.
In an exemplary system for antibody expression, a recombinant expression vector encoding both the antibody heavy chain and the antibody light chain of an anti-BDCA2 antibody (e.g., BIIB059) is introduced into dhfr− CHO cells by calcium phosphate-mediated transfection. Within the recombinant expression vector, the antibody heavy and light chain genes are each operatively linked to enhancer/promoter regulatory elements (e.g., derived from SV40, CMV, adenovirus and the like, such as a CMV enhancer/AdMLP promoter regulatory element or an SV40 enhancer/AdMLP promoter regulatory element) to drive high levels of transcription of the genes. The recombinant expression vector also carries a DHFR gene, which allows for selection of CHO cells that have been transfected with the vector using methotrexate selection/amplification. The selected transformant host cells are cultured to allow for expression of the antibody heavy and light chains and the antibody is recovered from the culture medium.
Antibodies can also be produced by a transgenic animal. For example, U.S. Pat. No. 5,849,992 describes a method of expressing an antibody in the mammary gland of a transgenic mammal A transgene is constructed that includes a milk-specific promoter and nucleic acids encoding the antibody of interest and a signal sequence for secretion. The milk produced by females of such transgenic mammals includes, secreted-therein, the antibody of interest. The antibody can be purified from the milk, or for some applications, used directly. Animals are also provided comprising one or more of the nucleic acids described herein.
The antibodies of the present disclosure can be isolated from inside or outside (such as medium) of the host cell and purified as substantially pure and homogenous antibodies. Methods for isolation and purification commonly used for antibody purification may be used for the isolation and purification of antibodies, and are not limited to any particular method. Antibodies may be isolated and purified by appropriately selecting and combining, for example, column chromatography, filtration, ultrafiltration, salting out, solvent precipitation, solvent extraction, distillation, immunoprecipitation, SDS-polyacrylamide gel electrophoresis, isoelectric focusing, dialysis, and recrystallization. Chromatography includes, for example, affinity chromatography, ion exchange chromatography, hydrophobic chromatography, gel filtration, reverse-phase chromatography, and adsorption chromatography (Strategies for Protein Purification and Characterization: A Laboratory Course Manual. Ed Daniel R. Marshak et al., Cold Spring Harbor Laboratory Press, 1996). Chromatography can be carried out using liquid phase chromatography such as HPLC and FPLC. Columns used for affinity chromatography include protein A column and protein G column. Examples of columns using protein A column include Hyper D, POROS, and Sepharose FF (GE Healthcare Biosciences). The present disclosure also includes antibodies that are highly purified using these purification methods.
Characterization of the Antibodies
The BDCA2-binding properties of the antibodies described herein may be measured by any standard method, e.g., one or more of the following methods: OCTET®, Surface Plasmon Resonance (SPR), BIACORETM analysis, Enzyme Linked Immunosorbent Assay (ELISA), EIA (enzyme immunoassay), RIA (radioimmunoassay), and Fluorescence Resonance Energy Transfer (FRET).
The binding interaction of a protein of interest (an anti-BDCA2 antibody) and a target (e.g., BDCA2) can be analyzed using the OCTET® systems. In this method, one of several variations of instruments (e.g., OCTET® QKe and QK), made by the FortéBio company are used to determine protein interactions, binding specificity, and epitope mapping. The OCTET® systems provide an easy way to monitor real-time binding by measuring the changes in polarized light that travels down a custom tip and then back to a sensor.
The binding interaction of a protein of interest (an anti-BDCA2 antibody) and a target (e.g., BDCA2) can be analyzed using Surface Plasmon Resonance (SPR). SPR or Biomolecular Interaction Analysis (BIA) detects biospecific interactions in real time, without labeling any of the interactants. Changes in the mass at the binding surface (indicative of a binding event) of the BIA chip result in alterations of the refractive index of light near the surface (the optical phenomenon of surface plasmon resonance (SPR)). The changes in the refractivity generate a detectable signal, which are measured as an indication of real-time reactions between biological molecules. Methods for using SPR are described, for example, in U.S. Pat. No. 5,641,640; Raether (1988) Surface Plasmons Springer Verlag; Sjolander and Urbaniczky (1991) Anal. Chem. 63:2338-2345; Szabo et al. (1995) Curr. Opin. Struct. Biol. 5:699-705 and on-line resources provide by BIAcore International AB (Uppsala, Sweden). Information from SPR can be used to provide an accurate and quantitative measure of the equilibrium dissociation constant (Kd), and kinetic parameters, including Kon and Koff, for the binding of a biomolecule to a target.
Epitopes can also be directly mapped by assessing the ability of different antibodies to compete with each other for binding to human BDCA2 using BIACORE chromatographic techniques (Pharmacia BIAtechnology Handbook, “Epitope Mapping”, Section 6.3.2, (May 1994); see also Johne et al. (1993) J. Immunol. Methods, 160:191-198).
When employing an enzyme immunoassay, a sample containing an antibody, for example, a culture supernatant of antibody-producing cells or a purified antibody is added to an antigen-coated plate. A secondary antibody labeled with an enzyme such as alkaline phosphatase is added, the plate is incubated, and after washing, an enzyme substrate such as p-nitrophenylphosphate is added, and the absorbance is measured to evaluate the antigen binding activity.
Additional general guidance for evaluating antibodies, e.g., Western blots and immunoprecipitation assays, can be found in Antibodies: A Laboratory Manual, ed. by Harlow and Lane, Cold Spring Harbor press (1988)).
Deposits
A hybridoma producing the anti-BDCA2 monoclonal antibody designated murine hybridoma BDCA2-1P24F4.1.1.1 has been deposited with the American Type Culture Collection (ATCC) under the terms of the Budapest Treaty on the International Recognition of the Deposit of Microorganisms for the Purpose of Patent Procedure on Jan. 15, 2013, and bears the accession number PTA-13450. Applicants acknowledge their duty to replace the deposits should the depository be unable to furnish a sample when requested due to the condition of the deposit before the end of the term of a patent issued hereon. Applicants also acknowledge their responsibility to notify the ATCC of the issuance of such a patent, at which time the deposit will be made available to the public. Prior to that time, the deposit will be made available to the Commissioner of Patents under the terms of 37 C.F.R. §1.14 and 35 U.S.C. §112.
Antibodies with Altered Effector Function
The interaction of antibodies and antibody-antigen complexes with cells of the immune system triggers a variety of responses, referred to herein as effector functions. Immune-mediated effector functions include two major mechanisms: antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). Both of them are mediated by the constant region of the immunoglobulin protein. The antibody Fc domain is, therefore, the portion that defines interactions with immune effector mechanisms.
IgG antibodies activate effector pathways of the immune system by binding to members of the family of cell surface Fcγ receptors and to C1q of the complement system. Ligation of effector proteins by clustered antibodies triggers a variety of responses, including release of inflammatory cytokines, regulation of antigen production, endocytosis, and cell killing. In some clinical applications these responses are crucial for the efficacy of a monoclonal antibody. In others they provoke unwanted side effects such as inflammation and the elimination of antigen-bearing cells. Accordingly, the present invention further relates to BDCA2-binding proteins, including antibodies, with altered, e.g., increased or reduced effector functions.
Effector function of an anti-BDCA2 antibody of the present invention may be determined using one of many known assays. The anti-BDCA2 antibody's effector function may be increased or reduced relative to a second anti-BDCA2 antibody. In some embodiments, the second anti-BDCA2 antibody may be any antibody that binds BDCA2 specifically. In other embodiments, the second BDCA2-specific antibody may be any of the antibodies of the invention, such as BIIB059. In other embodiments, where the anti-BDCA2 antibody of interest has been modified to increase or reduce effector function, the second anti-BDCA2 antibody may be the unmodified or parental version of the antibody.
Effector functions include antibody-dependent cell-mediated cytotoxicity (ADCC), whereby antibodies bind Fc receptors on cytotoxic T cells, natural killer (NK) cells, or macrophages leading to cell death, and complement-dependent cytotoxicity (CDC), which is cell death induced via activation of the complement cascade (reviewed in Daeron, Annu. Rev. Immunol., 15:203-234 (1997); Ward and Ghetie, Therapeutic Immunol., 2:77-94 (1995); and Ravetch and Kinet, Annu. Rev. Immunol. 9:457-492 (1991)). Such effector functions generally require the Fc region to be combined with a binding domain (e.g. an antibody variable domain) and can be assessed using standard assays that are known in the art (see, e.g., WO 05/018572, WO 05/003175, and U.S. Pat. No. 6,242,195).
Effector functions can be avoided by using antibody fragments lacking the Fc domain such as Fab, Fab′2, or single chain Fv. An alternative is to use the IgG4 subtype antibody, which binds to FcγRI but which binds poorly to C1q and FcγRII and RIII. The IgG2 subtype also has reduced binding to Fc receptors, but retains significant binding to the H131 allotype of FcγRIIa and to C1q. Thus, additional changes in the Fc sequence are required to eliminate binding to all the Fc receptors and to C1q.
Several antibody effector functions, including ADCC, are mediated by Fc receptors (FcRs), which bind the Fc region of an antibody. The affinity of an antibody for a particular FcR, and hence the effector activity mediated by the antibody, may be modulated by altering the amino acid sequence and/or post-translational modifications of the Fc and/or constant region of the antibody.
FcRs are defined by their specificity for immunoglobulin isotypes; Fc receptors for IgG antibodies are referred to as FcγR, for IgE as FcεR, for IgA as FcαR and so on. Three subclasses of FcγR have been identified: FcγRI (CD64), FcγRII (CD32) and FcγRIII (CD16). Both FcγRII and FcγRIII have two types: FcγRIIa (CD32a) and FcγRIIB (CD32b); and FcγRIIIA (CD16a) and FcγRIIIB (CD16b). Because each FcγR subclass is encoded by two or three genes, and alternative RNA splicing leads to multiple transcripts, a broad diversity in FcγR isoforms exists. For example, FcγRII (CD32) includes the isoforms IIa, IIb1, IIb2 IIb3, and IIc.
The binding site on human and murine antibodies for FcγR has been previously mapped to the so-called “lower hinge region” consisting of residues 233-239 (EU index numbering as in Kabat et al., Sequences of Proteins of Immunological Interest, 5th Ed. Public Health Service, National Institutes of Health, Bethesda, Md. (1991), Woof et al., Molec. Immunol. 23:319-330 (1986); Duncan et al., Nature 332:563 (1988); Canfield and Morrison, J. Exp. Med. 173:1483-1491 (1991); Chappel et al., Proc. Natl. Acad. Sci USA 88:9036-9040 (1991)). Of residues 233-239, P238 and 5239 are among those cited as possibly being involved in binding. Other previously cited areas possibly involved in binding to FcγR are: G316-K338 (human IgG) for human FcγRI (Woof et al., Mol. Immunol., 23:319-330 (1986)); K274-R301 (human IgG1) for human FcγRIII (Sarmay et al., Molec. Immunol. 21:43-51 (1984)); and Y407-R416 (human IgG) for human FcγRIII (Gergely et al., Biochem. Soc. Trans. 12:739-743 (1984) and Shields et al., J Biol Chem 276: 6591-6604 (2001), Lazar G A et al., Proc Natl Acad Sci 103: 4005-4010 (2006). These and other stretches or regions of amino acid residues involved in FcR binding may be evident to the skilled artisan from an examination of the crystal structures of Ig-FcR complexes (see, e.g., Sondermann et al. 2000 Nature 406(6793):267-73 and Sondermann et al. 2002 Biochem Soc Trans. 30(4):481-6). Accordingly, the anti-BDCA2 antibodies of the present invention include modifications of one or more of the aforementioned residues (to increase or decrease effector function as needed).
Another approach for altering monoclonal antibody effector function include mutating amino acids on the surface of the monoclonal antibody that are involved in effector binding interactions (Lund, J., et al. (1991) J. Immunol. 147(8): 2657-62; Shields, R. L. et al. (2001) J Biol. Chem. 276(9): 6591-604).
Methods of increasing effector function of antibodies are well known in the art (see, e.g., Kelley et al., Methods Mol. Biol., 901:277-93 (2012); Natsume et al., Drug Des Devel Ther., 3:7-16 (2009); U.S. Pat. No. 8,188,231, U.S. Pat. No. 7,960,512). In one embodiment, the BDCA2 antibodies have one, two, three, four, five, six, seven, or more amino acid substitutions at a position selected from the group consisting of 221, 222, 223, 224, 225, 227, 228, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 243, 244, 245, 246, 247, 249, 255, 258, 260, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 278, 280, 281, 282, 283, 284, 285, 286, 288, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 313, 317, 318, 320, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, and 337, wherein the numbering of the residues in the Fc region is that of the EU index as in Kabat. In certain embodiments, the BDCA2 antibodies have one, two, three, four, five, six, seven, or more of the amino acid substitutions selected from the group consisting of: D221K, D221Y, K222E, K222Y, T223E, T223K, H224E, H224Y, T225E, T225K, T225W, P227E, P227G, P227K, P227Y, P228E, P228G, P228K, P228Y, P230A, P230E, P230G, P230Y, A231E, A231G, A231K, A231P, A231Y, P232E, P232G, P232K, P232Y, E233A, E233D, E233F, E233G, E233H, E233I, E233K, E233L, E233M, E233N, E233Q, E233R, E233S, E233T, E233V, E233W, E233Y, L234A, L234D, L234E, L234F, L234G, L234H, L234I, L234K, L234M, L234N, L234P, L234Q, L234R, L234S, L234T, L234V, L234W, L234Y, L235A, L235D, L235E, L235F, L235G, L235H, L235I, L235K, L235M, L235N, L235P, L235Q, L235R, L235S, L235T, L235V, L235W, L235Y, G236A, G236D, G236E, G236F, G236H, G236I, G236K, G236L, G236M, G236N, G236P, G236Q, G236R, G236S, G236T, G236V, G236W, G236Y, G237D, G237E, G237F, G237H, G237I, G237K, G237L, G237M, G237N, G237P, G237Q, G237R, G237S, G237T, G237V, G237W, G237Y, P238D, P238E, P238F, P238G, P238H, P238I, P238K, P238L, P238M, P238N, P238Q, P238R, P238S, P238T, P238V, P238W, P238Y, S239D, S239E, S239F, S239G, S239H, S239I, S239K, S239L, S239M, S239N, S239P, S239Q, S239R, S239T, S239V, S239W, S239Y, V240A, V240I, V240M, V240T, F241D, F241E, F241L, F241R, F241S, F241W, F241Y, F243E, F243H, F243L, F243Q, F243R, F243W, F243Y, P244H, P245A, K246D, K246E, K246H, K246Y, P247G, P247V, D249H, D249Q, D249Y, R255E, R255Y, E258H, E258S, E258Y, T260D, T260E, T260H, T260Y, V262A, V262E, V262F, V262I, V262T, V263A, V263I, V263M, V263T, V264A, V264D, V264E, V264F, V264G, V264H, V264I, V264K, V264L, V264M, V264N, V264P, V264Q, V264R, V264S, V264T, V264W, V264Y, D265F, D265G, D265H, D265I, D265K, D265L, D265M, D265N, D265P, D265Q, D265R, D265S, D265T, D265V, D265W, D265Y, V266A, V266I, V266M, V266T, S267D, S267E, S267F, S267H, S267I, S267K, S267L, S267M, S267N, S267P, S267Q, S267R, S267T, S267V, S267W, S267Y, H268D, H268E, H268F, H268G, H268I, H268K, H268L, H268M, H268P, H268Q, H268R, H268T, H268V, H268W, E269F, E269G, E269H, E269I, E269K, E269L, E269M, E269N, E269P, E269R, E269S, E269T, E269V, E269W, E269Y, D270F, D270G, D270H, D270I, D270L, D270M, D270P, D270Q, D270R, D270S, D270T, D270W, D270Y, P271A, P271D, P271E, P271F, P271G, P271H, P271I, P271K, P271L, P271M, P271N, P271Q, P271R, P271S, P271T, P271V, P271W, P271Y, E272D, E272F, E272G, E272H, E272I, E272K, E272L, E272M, E272P, E272R, E272S, E272T, E272V, E272W, E272Y, V273I, K274D, K274E, K274F, K274G, K274H, K274I, K274L, K274M, K274N, K274P, K274R, K274T, K274V, K274W, K274Y, F275L, F275W, N276D, N276E, N276F, N276G, N276H, N276I, N276L, N276M, N276P, N276R, N276S, N276T, N276V, N276W, N276Y, Y278D, Y278E, Y278G, Y278H, Y278I, Y278K, Y278L, Y278M, Y278N, Y278P, Y278Q, Y278R, Y278S, Y278T, Y278V, Y278W, D280G, D280K, D280L, D280P, D280W, G281D, G281E, G281K, G281N, G281P, G281Q, G281Y, V282E, V282G, V282K, V282P, V282Y, E283G, E283H, E283K, E283L, E283P, E283R, E283Y, V284D, V284E, V284L, V284N, V284Q, V284T, V284Y, H285D, H285E, H285K, H285Q, H285W, H285Y, N286E, N286G, N286P, N286Y, K288D, K288E, K288Y, K290D, K290H, K290L, K290N, K290W, P291D, P291E, P291G, P291H, P291I, P291Q, P291T, R292D, R292E, R292T, R292Y, E293F, E293G, E293H, E293I, E293L, E293M, E293N, E293P, E293R, E293S, E293T, E293V, E293W, E293Y, E294F, E294G, E294H, E294I, E294K, E294L, E294M, E294P, E294R, E294S, E294T, E294V, E294W, E294Y, Q295D, Q295E, Q295F, Q295G, Q295H, Q295I, Q295M, Q295N, Q295P, Q295R, Q295S, Q295T, Q295V, Q295W, Q295Y, Y296A, Y296D, Y296E, Y296G, Y296H, Y296I, Y296K, Y296L, Y296M, Y296N, Y296Q, Y296R, Y296S, Y296T, Y296V, N297D, N297E, N297F, N297G, N297H, N297I, N297K, N297L, N297M, N297P, N297Q, N297R, N297S, N297T, N297V, N297W, N297Y, S298D, S298E, S298F, S298H, S298I, S298K, S298M, S298N, S298Q, S298R, S298T, S298W, S298Y, T299A, T299D, T299E, T299F, T299G, T299H, T299I, T299K, T299L, T299M, T299N, T299P, T299Q, T299R, T299S, T299V, T299W, T299Y, Y300A, Y300D, Y300E, Y300G, Y300H, Y300K, Y300M, Y300N, Y300P, Y300Q, Y300R, Y300S, Y300T, Y300V, Y300W, R301D, R301E, R301H, R301Y, V302I, V303D, V303E, V303Y, S304D, S304H, S304L, S304N, S304T, V305E, V305T, V305Y, W313F, K317E, K317Q, E318H, E318L, E318Q, E318R, E318Y, K320D, K320F, K320G, K320H, K320I, K320L, K320N, K320P, K320S, K320T, K320V, K320W, K320Y, K322D, K322F, K322G, K322H, K322I, K322P, K322S, K322T, K322V, K322W, K322Y, V323I, S324D, S324F, S324G, S324H, S324I, S324L, S324M, S324P, S324R, S324T, S324V, S324W, S324Y, N325A, N325D, N325E, N325F, N325G, N325H, N325I, N325K, N325L, N325M, N325P, N325Q, N325R, N325S, N325T, N325V, N325W, N325Y, K326I, K326L, K326P, K326T, A327D, A327E, A327F, A327H, A327I, A327K, A327L, A327M, A327N, A327P, A327R, A327S, A327T, A327V, A327W, A327Y, L328A, L328D, L328E, L328F, L328G, L328H, L328I, L328K, L328M, L328N, L328P, L328Q, L328R, L328S, L328T, L328V, L328W, L328Y, P329D, P329E, P329F, P329G, P329H, P329I, P329K, P329L, P329M, P329N, P329Q, P329R, P329S, P329T, P329V, P329W, P329Y, A330E, A330F, A330G, A330H, A330I, A330L, A330M, A330N, A330P, A330R, A330S, A330T, A330V, A330W, A330Y, P331D, P331F, P331H, P331I, P331L, P331M, P331Q, P331R, P331T, P331V, P331W, P331Y, I332A, I332D, I332E, I332F, I332H, I332K, I332L, I332M, I332N, I332P, I332Q, I332R, I332S, I332T, I332V, I332W, I332Y, E333F, E333H, E333I, E333L, E333M, E333P, E333T, E333Y, K334F, K334I, K334L, K334P, K334T, T335D, T335F, T335G, T335H, T335I, T335L, T335M, T335N, T335P, T335R, T335S, T335V, T335W, T335Y, I336E, I336K, I336Y, S337E, S337H, and S337N, wherein the numbering of the residues in the Fc region is that of the EU index as in Kabat. In a particular embodiment, the BDCA2 antibodies comprise one, two, or three of the following mutations: S239D, S239D/I332E, S239D/I332E/A330L, S239D/I332E/G236A, S298A, A330L I332E, E333A, and K334A.
The presence of oligosaccharides—specifically, the N-linked oligosaccharide at asparigine-297 in the CH2 domain of IgG1—is important for binding to FcγR as well as C1q. Reducing the fucose content of antibodies improves effector function (see, e.g., U.S. Pat. No. 8,163,551). In certain embodiments the BDCA2 antibodies have reduced fucosylation and amino acid substitutions that increase effector function (e.g., one, two, or three of the following mutations: S298A; E333A, and K334A). Effector function can also be achieved by preparing and expressing the anti-BDCA2 antibodies described herein in the presence of alpha-mannosidase I inhibitors (e.g., kifunensine) at a concentration of the inhibitor of about 60-200 ng/mL (e.g., 60 ng/mL, 75 ng/mL, 100 ng/mL, 150 g/ml). Antibodies expressed in the presence of alpha-mannosidase I inhibitors contain mainly oligomannose-type glycans and generally demonstrate increased ADCC activity and affinity for FcγRIIIA, but reduced C1q binding.
Anti-BDCA2 antibodies of the present disclosure with increased effector function include antibodies with increased binding affinity for one or more Fc receptors (FcRs) relative to a parent or non-variant anti-BDCA2 antibody. Accordingly, anti-BDCA2 antibodies with increased FcR binding affinity includes anti-BDCA2 antibodies that exhibit a 1.5-fold, 2-fold, 2.5-fold, 3-fold, 4-fold, or 5-fold or higher increase in binding affinity to one or more Fc receptors compared to a parent or non-variant anti-BDCA2 antibody. In some embodiments, an anti-BDCA2 antibody with increased effector function binds to an FcR with about 10-fold greater affinity relative to a parent or non-variant antibody. In other embodiments, an anti-BDCA2 antibody with increased effector function binds to an FcR with about 15-fold greater affinity or with about 20-fold greater affinity relative to a parent or non-variant antibody. The FcR receptor may be one or more of FcγRI (CD64), FcγRII (CD32), and FcγRIII, and isoforms thereof, and FcεR, FcμR, FcδR, and/or an FcαR. In particular embodiments, an anti-BDCA2 antibody with increased effector function exhibits a 1.5-fold, 2-fold, 2.5-fold, 3-fold, 4-fold, or 5-fold or higher increase in binding affinity to FcγRIIa.
To reduce effector function, one can use combinations of different subtype sequence segments (e.g., IgG2 and IgG4 combinations) to give a greater reduction in binding to Fcγ receptors than either subtype alone (Armour et al., Eur. J. Immunol., 29:2613-1624 (1999); Mol. Immunol., 40:585-593 (2003)). In addition, sites of N-linked glycosylation can be removed as a means of reducing effector function. A large number of Fc variants having altered and/or reduced affinities for some or all Fc receptor subtypes (and thus for effector functions) are known in the art. See, e.g., US 2007/0224188; US 2007/0148171; US 2007/0048300; US 2007/0041966; US 2007/0009523; US 2007/0036799; US 2006/0275283; US 2006/0235208; US 2006/0193856; US 2006/0160996; US 2006/0134105; US 2006/0024298; US 2005/0244403; US 2005/0233382; US 2005/0215768; US 2005/0118174; US 2005/0054832; US 2004/0228856; US 2004/132101; US 2003/158389; see also U.S. Pat. Nos. 7,183,387; 6,737,056; 6,538,124; 6,528,624; 6,194,551; 5,624,821; 5,648,260.
Anti-BDCA2 antibodies of the present invention with reduced effector function include antibodies with reduced binding affinity for one or more Fc receptors (FcRs) relative to a parent or non-variant anti-BDCA2 antibody. Accordingly, anti-BDCA2 antibodies with reduced FcR binding affinity includes anti-BDCA2 antibodies that exhibit a 1.5-fold, 2-fold, 2.5-fold, 3-fold, 4-fold, or 5-fold or higher decrease in binding affinity to one or more Fc receptors compared to a parent or non-variant anti-BDCA2 antibody. In some embodiments, an anti-BDCA2 antibody with reduced effector function binds to an FcR with about 10-fold less affinity relative to a parent or non-variant antibody. In other embodiments, an anti-BDCA2 antibody with reduced effector function binds to an FcR with about 15-fold less affinity or with about 20-fold less affinity relative to a parent or non-variant antibody. The FcR receptor may be one or more of FcγRI (CD64), FcγRII (CD32), and FcγRIII, and isoforms thereof, and FcεR, FcμR, FcδR, and/or an FcαR. In particular embodiments, an anti-BDCA2 antibody with reduced effector function exhibits a 1.5-fold, 2-fold, 2.5-fold, 3-fold, 4-fold, or 5-fold or higher decrease in binding affinity to FcγRIIa.
In CDC, the antibody-antigen complex binds complement, resulting in the activation of the complement cascade and generation of the membrane attack complex. Activation of the classical complement pathway is initiated by the binding of the first component of the complement system (C1q) to antibodies (of the appropriate subclass) which are bound to their cognate antigen; thus the activation of the complement cascade is regulated in part by the binding affinity of the immunoglobulin to C1q protein. To activate the complement cascade, it is necessary for C1q to bind to at least two molecules of IgG1, IgG2, or IgG3, but only one molecule of IgM, attached to the antigenic target (Ward and Ghetie, Therapeutic Immunology 2:77-94 (1995) p. 80). To assess complement activation, a CDC assay, e.g. as described in Gazzano-Santoro et al., J. Immunol. Methods, 202:163 (1996), may be performed.
It has been proposed that various residues of the IgG molecule are involved in binding to C1q including the Glu318, Lys320 and Lys322 residues on the CH2 domain, amino acid residue 331 located on a turn in close proximity to the same beta strand, the Lys235 and Gly237 residues located in the lower hinge region, and residues 231 to 238 located in the N-terminal region of the CH2 domain (see e.g., Xu et al., J. Immunol. 150:152A (Abstract) (1993), WO94/29351; Tao et al, J. Exp. Med., 178:661-667 (1993); Brekke et al., Eur. J. Immunol., 24:2542-47 (1994); Burton et al; Nature, 288:338-344 (1980); Duncan and Winter, Nature 332:738-40 (1988); Idusogie et al J Immunol 164: 4178-4184 (2000; U.S. Pat. No. 5,648,260, and U.S. Pat. No. 5,624,821).
Ant-BDCA2 antibodies with improved C1q binding can comprise an amino acid substitution at one, two, three, or four of amino acid positions 326, 327, 333 and 334 of the human IgG Fc region, where the numbering of the residues in the IgG Fc region is that of the EU index as in Kabat. In one embodiment, the anti-BDCA2 antibodies include the following amino acid substitutions: K326W/E333S, which are known to increase binding of an IgG1 antibody to C1q (Steurer W. et al., J Immunol., 155(3):1165-74 (1995)).
Ant-BDCA2 antibodies with reduced C1q binding can comprise an amino acid substitution at one, two, three, or four of amino acid positions 270, 322, 329 and 331 of the human IgG Fc region, where the numbering of the residues in the IgG Fc region is that of the EU index as in Kabat. As an example in IgG1, two mutations in the COOH terminal region of the CH2 domain of human IgG1—K322A and P329A—do not activate the CDC pathway and were shown to result in more than a 100 fold decrease in C1q binding (U.S. Pat. No. 6,242,195).
Accordingly, in certain embodiments, an anti-BDCA2 antibody of the present invention exhibits increased or reduced binding to a complement protein relative to a second anti-BDCA2 antibody. In certain embodiments, an anti-BDCA2 antibody of the invention exhibits increased or reduced binding to C1q by a factor of about 1.5-fold or more, about 2-fold or more, about 3-fold or more, about 4-fold or more, about 5-fold or more, about 6-fold or more, about 7-fold or more, about 8-fold or more, about 9-fold or more, about 10-fold or more, or about 15-fold or more, relative to a second anti-BDCA2 antibody.
Thus, in certain embodiments of the invention, one or more of these residues may be modified, substituted, or removed or one or more amino acid residues may be inserted so as to increase or decrease CDC activity of the anti-BDCA2 antibodies provided herein.
In certain other embodiments, the present invention provides an anti-BDCA2 antibody that exhibits reduced binding to one or more FcR receptors but that maintains its ability to bind complement (e.g., to a similar or, in some embodiments, to a lesser extent than a native, non-variant, or parent anti-BDCA2 antibody). Accordingly, an anti-BDCA2 antibody of the present invention may bind and activate complement while exhibiting reduced binding to an FcR, such as, for example, FcγRIIa (e.g., FcγRIIa expressed on platelets). Such an antibody with reduced or no binding to FcγRIIa (such as FcγRIIa expressed on platelets, for example) but that can bind C1q and activate the complement cascade to at least some degree will reduce the risk of thromboembolic events while maintaining perhaps desirable effector functions. In alternative embodiments, an anti-BDCA2 antibody of the present invention exhibits reduced binding to one or more FcRs but maintains its ability to bind one or more other FcRs. See, for example, US 2007-0009523, 2006-0194290, 2005-0233382, 2004-0228856, and 2004-0191244, which describe various amino acid modifications that generate antibodies with reduced binding to FcRI, FcRII, and/or FcRIII, as well as amino acid substitutions that result in increased binding to one FcR but decreased binding to another FcR.
Accordingly, effector functions involving the constant region of an anti-BDCA2 antibody may be modulated by altering properties of the constant region, and the Fc region in particular. In certain embodiments, the anti-BDCA2 antibody having increased or decreased effector function is compared with a second antibody with effector function and which may be a non-variant, native, or parent antibody comprising a native constant or Fc region that mediates effector function.
A native sequence Fc or constant region comprises an amino acid sequence identical to the amino acid sequence of a Fc or constant chain region found in nature. Preferably, a control molecule used to assess relative effector function comprises the same type/subtype Fc region as does the test or variant antibody. A variant or altered Fc or constant region comprises an amino acid sequence which differs from that of a native sequence heavy chain region by virtue of at least one amino acid modification (such as, for example, post-translational modification, amino acid substitution, insertion, or deletion). Accordingly, the variant constant region may contain one or more amino acid substitutions, deletions, or insertions that results in altered post-translational modifications, including, for example, an altered glycosylation pattern. A parent antibody or Fc region is, for example, a variant having normal effector function used to construct a constant region (i.e., Fc) having altered, e.g., increased effector function.
Antibodies with altered (e.g., increased) effector function(s) may be generated by engineering or producing antibodies with variant constant, Fc, or heavy chain regions. Recombinant DNA technology and/or cell culture and expression conditions may be used to produce antibodies with altered function and/or activity. For example, recombinant DNA technology may be used to engineer one or more amino acid substitutions, deletions, or insertions in regions (such as, for example, Fc or constant regions) that affect antibody function including effector functions. Alternatively, changes in post-translational modifications, such as, e.g. glycosylation patterns, may be achieved by manipulating the host cell and cell culture and expression conditions by which the antibody is produced.
Certain embodiments of the present invention relate to an anti-BDCA2 antibody comprising one or more heavy chain CDR sequences selected from VH CDR1 of SEQ ID NO:9, VH CDR2 of SEQ ID NO:10, and VH CDR3 of SEQ ID NO:11; or one or more heavy chain alternate CDR sequences selected from: VH CDR1 of SEQ ID NO:8, VH CDR2 of SEQ ID NO:10, and VH CDR3 of SEQ ID NO:11; or one or more heavy chain alternate CDR sequences selected from: VH CDR1 of SEQ ID NO:89, VH CDR2 of SEQ ID NO:91, and VH CDR3 of SEQ ID NO:11; or one or more heavy chain alternate CDR sequences selected from: VH CDR1 of SEQ ID NO:9, VH CDR2 of SEQ ID NO:92, and VH CDR3 of SEQ ID NO:11; or one or more heavy chain alternate CDR sequences selected from: VH CDR1 of SEQ ID NO:90, VH CDR2 of SEQ ID NO:93, and VH CDR3 of SEQ ID NO:94, wherein the antibody further comprises a variant Fc region that confers increased or reduced effector function compared to a native or parental Fc region. In further embodiments, the anti-BDCA2 antibody comprises at least two of the CDRs (or alternate CDRs), and in other embodiments the antibody comprises all three of the heavy chain CDR sequences (or alternate CDRs). These anti-BDCA2 antibodies i) inhibit secretion of type I interferons and/or type III interferons in addition to other cytokines and chemokines from plasmacytoid dendritic cells; and/or (ii) induce or enhance depletion of plasmacytoid dendritic cells in vitro.
Other embodiments of the present invention relate to an anti-BDCA2 antibody comprising one or more light chain CDR sequences selected from VL CDR1 of SEQ ID NO:5, VL CDR2 of SEQ ID NO:6, and VL CDR3 of SEQ ID NO:7; or one or more light chain alternate CDR sequences selected from VL CDR1 of SEQ ID NO:95, VL CDR2 of SEQ ID NO:96, and VL CDR3 of SEQ ID NO:97, the antibody further comprising a variant Fc region that confers increased or reduced effector function compared to a native or parental Fc region. In further embodiments, the anti-BDCA2 antibody comprises at least two of the light chain CDRs (or alternate CDRs), and in other embodiments the antibody comprises all three of the light chain CDR sequences (or alternate CDRs). These anti-BDCA2 antibodies i) inhibit secretion of type I interferons and/or type III interferons in addition to other cytokines and chemokines from plasmacytoid dendritic cells; and/or (ii) induce or enhance depletion of plasmacytoid dendritic cells in vitro.
In further embodiments of the present invention, the anti-BDCA2 antibody with increased or reduced effector function comprises all three light chain CDR sequences or alternate light chain CDRs of SEQ ID NO:3 and comprises all three heavy chain CDR sequences or alternate heavy chain CDRs of SEQ ID NO:4
In other embodiments, the invention relates to an anti-BDCA2 antibody comprising a VL sequence comprising SEQ ID NO:23, the antibody further comprising a variant Fc region that confers reduced effector function compared to a native or parental Fc region. In yet other embodiments, the invention relates to an anti-BDCA2 antibody comprising a VH sequence comprising SEQ ID NO:24, the antibody further comprising a variant Fc region that confers reduced effector function compared to a native or parental Fc region.
Methods of generating any of the aforementioned anti-BDCA2 antibody variants comprising amino acid substitutions are well known in the art. These methods include, but are not limited to, preparation by site-directed (or oligonucleotide-mediated) mutagenesis, PCR mutagenesis, and cassette mutagenesis of a prepared DNA molecule encoding the antibody or at least the constant region of the antibody. Site-directed mutagenesis is well known in the art (see, e.g., Carter et al., Nucleic Acids Res., 13:4431-4443 (1985) and Kunkel et al., Proc. Natl. Acad. Sci. USA, 82:488 (1987)). PCR mutagenesis is also suitable for making amino acid sequence variants of the starting polypeptide. See Higuchi, in PCR Protocols, pp. 177-183 (Academic Press, 1990); and Vallette et al., Nuc. Acids Res. 17:723-733 (1989). Another method for preparing sequence variants, cassette mutagenesis, is based on the technique described by Wells et al., Gene, 34:315-323 (1985).
Anti-BDCA2 Antibodies with Altered Glycosylation
Different glycoforms can profoundly affect the properties of a therapeutic, including pharmacokinetics, pharmacodynamics, receptor-interaction and tissue-specific targeting (Graddis et al., 2002, Curr Pharm Biotechnol. 3: 285-297). In particular, for antibodies, the oligosaccharide structure can affect properties relevant to protease resistance, the serum half-life of the antibody mediated by the FcRn receptor, phagocytosis and antibody feedback, in addition to effector functions of the antibody (e.g., binding to the complement complex C1, which induces CDC, and binding to FcγR receptors, which are responsible for modulating the ADCC pathway) (Nose and Wigzell, 1983; Leatherbarrow and Dwek, 1983; Leatherbarrow et al., 1985; Walker et al., 1989; Carter et al., 1992, PNAS, 89: 4285-4289).
Accordingly, another means of modulating effector function of antibodies includes altering glycosylation of the antibody constant region. Altered glycosylation includes, for example, a decrease or increase in the number of glycosylated residues, a change in the pattern or location of glycosylated residues, as well as a change in sugar structure(s). The oligosaccharides found on human IgGs affects their degree of effector function (Raju, T. S. BioProcess International April 2003. 44-53); the microheterogeneity of human IgG oligosaccharides can affect biological functions such as CDC and ADCC, binding to various Fc receptors, and binding to C1q protein (Wright A. & Morrison S L. TIBTECH 1997, 15 26-32; Shields et al. J Biol Chem. 2001 276(9):6591-604; Shields et al. J Biol Chem. 2002; 277(30):26733-40; Shinkawa et al. J Biol Chem. 2003 278(5):3466-73; Umana et al. Nat Biotechnol. 1999 February; 17(2): 176-80). For example, the ability of IgG to bind C1q and activate the complement cascade may depend on the presence, absence or modification of the carbohydrate moiety positioned between the two CH2 domains (which is normally anchored at Asn297) (Ward and Ghetie, Therapeutic Immunology 2:77-94 (1995).
Glycosylation sites in an Fc-containing polypeptide, for example an antibody such as an IgG antibody, may be identified by standard techniques. The identification of the glycosylation site can be experimental or based on sequence analysis or modeling data. Consensus motifs, that is, the amino acid sequence recognized by various glycosyl transferases, have been described. For example, the consensus motif for an N-linked glycosylation motif is frequently NXT or NXS, where X can be any amino acid except proline. Several algorithms for locating a potential glycosylation motif have also been described. Accordingly, to identify potential glycosylation sites within an antibody or Fc-containing fragment, the sequence of the antibody is examined, for example, by using publicly available databases such as the website provided by the Center for Biological Sequence Analysis (see NetNGlyc services for predicting N-linked glycosylation sites and NetOGlyc services for predicting O-linked glycosylation sites).
In vivo studies have confirmed the reduction in the effector function of aglycosyl antibodies. For example, an aglycosyl anti-CD8 antibody is incapable of depleting CD8-bearing cells in mice (Isaacs, 1992 J. Immunol. 148: 3062) and an aglycosyl anti-CD3 antibody does not induce cytokine release syndrome in mice or humans (Boyd, 1995 supra; Friend, 1999 Transplantation 68:1632). Aglycosylated forms of the BDCA2 antibody also have reduced effector function.
Importantly, while removal of the glycans in the CH2 domain appears to have a significant effect on effector function, other functional and physical properties of the antibody remain unaltered. Specifically, it has been shown that removal of the glycans had little to no effect on serum half-life and binding to antigen (Nose, 1983 supra; Tao, 1989 supra; Dorai, 1991 supra; Hand, 1992 supra; Hobbs, 1992 Mol. Immunol. 29:949).
The anti-BDCA2 antibodies of the present invention may be modified or altered to elicit increased or decreased effector function(s) (compared to a second BDCA2-specific antibody). Methods for altering glycosylation sites of antibodies are described, e.g., in U.S. Pat. No. 6,350,861 and U.S. Pat. No. 5,714,350, WO 05/18572 and WO 05/03175; these methods can be used to produce anti-BDCA2 antibodies of the present invention with altered, reduced, or no glycosylation.
Indications
An anti-BDCA2 antibody described herein can be used to treat or prevent a variety of immunological disorders, such as inflammatory and autoimmune disorders. Anti-BDCA2 antibodies are useful to treat or prevent such disorders at least because they disable or deplete pDCs, and/or inhibit inflammatory cytokines and chemokines produced by pDCs, and/or downregulate CD32a, and/or inhibiting immune complex stimulation of pDCs, and/or downregulate or cause shedding of CD62L. The anti-BDCA2 antibodies of this disclosure can be combined with an antimalarial agent (e.g., HCQ) for improved therapeutic effects in the treatment of inflammatory and autoimmune disorders. Anti-BDCA2 antibodies can be used to reduce levels of cytokines and chemokines such as: type I interferons, type III interferons, IL-6, TNF-α, MIP1-α and MIP1-β, CCL5, and IP-10. Type I IFNs constitute a multiple-member family of cytokines, including 13 IFN-α subtypes, IFN-β, -ε, -κ, -ω, -δ and -τ. (Theofilopoulos, Annu. Rev. Immunol., 23:307-36 (2005)). Type III interferons consist of three IFN-λ, molecules called IFN-λ1, IFN-λ2 and IFN-λ3 (also referred to as IL29, IL28A and IL28B, respectively). By depleting and/or dampening pDC function, the anti-BDCA2 antibodies described herein provide a more robust treatment approach than treatments attempting to reduce specific IFN subtypes with neutralizing antibodies. In addition, the pDC-focused treatment approach of the anti-BDCA2 antibodies is more selective and potentially safer than global blockade of the IFN response. For example, anti-BDCA2 antibodies described herein effectively eliminate pDC-derived type I IFNs while maintaining other sources of IFN that could be necessary in the event of viral infections.
The term “treating” refers to administering a composition described herein in an amount, manner, and/or mode effective to improve a condition, symptom, or parameter associated with a disorder or to prevent progression or exacerbation of the disorder (including secondary damage caused by the disorder) to either a statistically significant degree or to a degree detectable to one skilled in the art.
Diseases or conditions that can be treated with an anti-BDCA2 antibody described herein include, e.g., systematic lupus erythematosus (SLE) (e.g., moderate or severe lupus), cutaneous lupus, discoid lupus, lupus nephritis, systemic sclerosis (scleroderma), morphea, psoriasis, rheumatoid arthritis, inflammatory bowel disease (IBD), deratomyositis, polymyositis, and type I diabetes.
SLE is a chronic autoimmune disease where multiple organs are damaged by immune complexes and tissue-binding autoantibodies (see, Guidelines for Referral and Management of Systemic Lupus Erythematosus in Adults, Arthritis & Rheumatism, 42(9):1785-1795 (1999)). Autoantibodies are present in SLE and may precede the development of the clinical disease (Arbuckle et al., N Engl. J. Med., 349(16):1526-33 (2003)). Internalization of the autoantibody containing immune complexes through Fc receptors leads to the production of type I interferon which in turn promotes loss of tolerance, perpetuating the vicious cycle of autoimmunity (Means et al., Ann N Y Acad Sci., 1062:242-51 (2005)). SLE is heterogeneous with regard to its clinical presentation, course, prognosis and genetics. African Americans share an increased risk for SLE that is often more severe as compared to white patients. Complement deficiencies were recognized early as risk factors for the development of SLE. More recently, genetic polymorphisms associated with type I interferon pathways have been described to confer susceptibility. For example, anti-double stranded DNA and anti-Ro autoantibodies were associated with a certain haplotype of the transcription factor interferon regulatory factor 5 (IRFS). The haplotype also predicted high levels of IFN-α in the serum of SLE patients (Niewold et al., Ann. Rheum. Dis., 71(3):463-8 (2012)). Higher IFN-α levels have been correlated with a greater extent of multiple organ involvement in SLE patients (Bengtsson et al., Lupus, 9(9):664-71 (2000)). Furthermore, the so called “interferon signature” seems to be prominent in SLE. Interferon signature represents an mRNA expression pattern of interferon inducible genes. A type-I interferon signature is found in more than half of SLE patients and is associated with greater disease activity (Baechler et al., Proc. Natl. Acad. Sci USA, 100(5):2610-5 (2003)). IFN-α monoclonal antibodies have now entered the clinics and phase 1 results of sifalimumab and rontalizumab have demonstrated a dose-dependent reduction in type I IFN signature in the whole blood of SLE patients (McBride et al., Arthritis Rheum., 64(11):3666-76 (2012); Yao et al., Arthritis Rheum., (6):1785-96 (2009)). Validated indices have been developed for the assessment of disease activity and disease severity (e.g., moderate, severe) (see, e.g., Gladman, Prognosis and treatment of systemic lupus erythematosus, Curr. Opin. Rheumatol., 8:430-437 (1996); Kalunian et al., Definition, classification, activity and damage indices. In: Dubois' lupus eyrthematosus. 5th ed., Baltimore: Williams and Wilkins; pp. 19-30 (1997)).
Systemic sclerosis or systemic scleroderma is a systemic autoimmune disease or systemic connective tissue disease that is a subtype of scleroderma. It is characterized by deposition of collagen in the skin and, less commonly, in the kidneys, heart, lungs & stomach. The female to male ratio for this disease is 4:1. The peak age of onset of the disease is between 30-50 years.
Psoriasis is an autoimmune disease that affects the skin. It occurs when the immune system mistakes the skin cells as a pathogen, and sends out faulty signals that speed up the growth cycle of skin cells. Psoriasis has been linked to an increased risk of stroke, and treating high blood lipid levels may lead to improvement. There are five types of psoriasis: plaque, guttate, inverse, pustular, and erythrodermic. The most common form, plaque psoriasis, is commonly seen as red and white hues of scaly patches appearing on the top first layer of the epidermis. However, some patients have no dermatological signs or symptoms.
Rheumatoid arthritis is a chronic inflammatory disorder that affects many tissues and organs, but principally attacks flexible joints. The process involves an inflammatory response of the capsule around the joints secondary to swelling of synovial cells, excess synovial fluid, and the development of fibrous tissue (pannus) in the synovium. The pathology of the disease process often leads to the destruction of articular cartilage and ankylosis of the joints. Rheumatoid arthritis can also produce diffuse inflammation in the lungs, membrane around the heart (pericardium), the membranes of the lung (pleura), and white of the eye (sclera), and also nodular lesions, most common in subcutaneous tissue. Although the cause of rheumatoid arthritis is unknown, autoimmunity plays a pivotal role in both its chronicity and progression, and RA is considered a systemic autoimmune disease. Over expression of TNFα and other proinflammatory cytokines has been observed in patients with arthritis (Feldmann et. al., Prog Growth Factor Res., 4:247-55 (1992)). Furthermore, transgenic animals that over express human TNFα develop an erosive polyarthritis with many characteristics associated with the disease (Keffer et. al., EMBO J., 10(13):4025-31 (1991)). Analgesia and anti-inflammatory drugs, including steroids, are used to suppress the symptoms, while disease-modifying antirheumatic drugs (DMARDs) are required to inhibit or halt the underlying immune process and prevent long-term damage. More recently, anti-TNFα antibody therapy (Rituximab) has been used to manage the disease (Edwards, et. al., N. Engl. J. Med., 350(25): 2572-81 (2004)).
Inflammatory bowel disease (IBD) is a group of inflammatory conditions of the colon and small intestine. The major types of IBD are Crohn's disease and ulcerative colitis (UC). The main difference between Crohn's disease and UC is the location and nature of the inflammatory changes: Crohn's disease can affect any part of the gastrointestinal tract, from mouth to anus (skip lesions), although a majority of the cases start in the terminal ileum; whereas, UC is restricted to the colon and the rectum. Depending on the level of severity, IBD may require immunosuppression to control the symptom, such as prednisone, TNF inhibition, azathioprine (Imuran), methotrexate, or 6-mercaptopurine. More commonly, treatment of IBD requires a form of mesalazine.
Dermatomyositis (DM) is a type of autoimmune connective-tissue disease related to polymyositis (PM) that is characterized by inflammation of the muscles and the skin. While DM most frequently affects the skin and muscles, it is a systemic disorder that may also affect the joints, the esophagus, the lungs, and, less commonly, the heart.
Polymyositis (PM) (“inflammation of many muscles”) is a type of chronic inflammation of the muscles (inflammatory myopathy) related to dermatomyositis and inclusion body myositis.
Type I diabetes is a form of diabetes mellitus that results from autoimmune destruction of insulin-producing beta cells of the pancreas. The subsequent lack of insulin leads to increased blood and urine glucose. The classical symptoms are polyuria, polydipsia, polyphagia, and weight loss.
Examples of other diseases suitable for treatment with an anti-BDCA2 antibodies described herein include asthma, Behcet's disease, CREST syndrome, Crohn's disease, dermatomyositis, juvenile dermatomyositis, diabetes mellitus, discoid lupus erythematosus, pulmonary fibrosis, autoimmune glomerulonephritis, membranous glomerulopathy, juvenile rheumatoid arthritis (juvenile chronic arthritis), mixed connective tissue disease, multiple sclerosis, nephrotic syndrome, panniculitis, pemphigoid, pemphigus, pemphigus erythematosus, pemphigus foliaceus, pemphigus vulgaris, rheumatic polymyalgia, systemic sclerosis, progressive systemic sclerosis (scleroderma), morphea (localized scleroderma), multiple sclerosis, psoriasis, psoriatic arthritis, pulmonary fibrosis, Raynaud's phenomenon/syndrome, Sjogren's syndrome, and ulcerative colitis.
A subject who is at risk for, diagnosed with, or who has one of these disorders can be administered an anti-BDCA2 antibody in an amount and for a time to provide an overall therapeutic effect. The anti-BDCA2 antibody can be administered alone (monotherapy) or in combination with other agents (combination therapy). In one embodiment, the agent for use in combination therapy with an anti-BDCA2 antibody described herein is an antimalarial agent. In one embodiment, the agent for use in combination therapy with an anti-BDCA2 antibody described herein is a TLR7 and/or TLR9 signaling inhibitor. In another embodiment, the agent for use in combination therapy with an anti-BDCA2 antibody described herein is a corticosteroid. In certain embodiments, the agent for use in combination therapy with an anti-BDCA2 antibody described herein is an anti-malarial drug and/or a kinase inhibitor (e.g., BTK inhibitor (e.g., ibrutinib (PCI-32765), AVI-292, ONO-WG-307), JAK1 inhibitor, JAK2 inhibitor, JAK3 inhibitor, Tyk2 inhibitor). In a specific embodiment, the agent for use in combination therapy with an anti-BDCA2 antibody described herein is hydroxychloroquine. The amounts and times of administration for combination therapies can be those that provide, e.g., an additive or a synergistic therapeutic effect. Further, the administration of the anti-BDCA2 antibody (with or without the second agent) can be used as a primary, e.g., first line treatment, or as a secondary treatment, e.g., for subjects who have an inadequate response to a previously administered therapy (i.e., a therapy other than one with an anti-BDCA2 antibody). In some embodiments, the combination therapy includes the use of an anti-BDCA2 antibody and one or more of the following agents: glucocorticoid, NSAID, prednisone, hydroxychloroquine, chloroquine, amodiaquine, pyrimethamine, proguanil, mefloquine, dapsone, primaquine, methotrexate, mycophenolate mofetil, azathioprine, thalidomide, cyclophosphamide, cyclosporine A, rapamycin, prostacyclin, phosphodiesterase inhibitor, endothelin antagonists, statin, ACE inhibitor, and calcium channel blockers. In other embodiments, the combination therapy includes the use of an anti-BDCA2 antibody and any one or more of: sulfasalazine, doxycycline, minocycline, penicillamine, tofacitinib, and leflunomide.
Pharmaceutical Compositions
An anti-BDCA2 antibody or antigen-binding fragment thereof described herein can be formulated as a pharmaceutical composition for administration to a subject, e.g., to treat a disorder described herein. Typically, a pharmaceutical composition includes a pharmaceutically acceptable carrier. As used herein, “pharmaceutically acceptable carrier” includes any and all solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents, and the like that are physiologically compatible. The composition can include a pharmaceutically acceptable salt, e.g., an acid addition salt or a base addition salt (see e.g., Berge, S. M., et al. (1977) J. Pharm. Sci. 66:1-19).
Pharmaceutical formulation is a well-established art, and is further described, e.g., in Gennaro (ed.), Remington: The Science and Practice of Pharmacy, 20th ed., Lippincott, Williams & Wilkins (2000) (ISBN: 0683306472); Ansel et al., Pharmaceutical Dosage Forms and Drug Delivery Systems, 7th Ed., Lippincott Williams & Wilkins Publishers (1999) (ISBN: 0683305727); and Kibbe (ed.), Handbook of Pharmaceutical Excipients American Pharmaceutical Association, 3rd ed. (2000) (ISBN: 091733096X).
The pharmaceutical compositions may be in a variety of forms. These include, for example, liquid, semi-solid and solid dosage forms, such as liquid solutions (e.g., injectable and infusible solutions), dispersions or suspensions, tablets, pills, powders, liposomes and suppositories. The preferred form can depend on the intended mode of administration and therapeutic application. Typically compositions for the agents described herein are in the form of injectable or infusible solutions.
In one embodiment, an anti-BDCA2 antibody described herein is formulated with excipient materials, such as sodium chloride, sodium citrate, sodium dibasic phosphate heptahydrate, sodium monobasic phosphate, Tween-80, and a stabilizer. It can be provided, for example, in a buffered solution at a suitable concentration and can be stored at 2-8° C. In some other embodiments, the pH of the composition is between about 5.8 and 6.6 (e.g., 5.8, 5.9, 6.0, 6.1, 6.2, 6.3, 6.4, 6.5, 6.6).
The pharmaceutical compositions can also include agents that reduce aggregation of the BDCA2 antibody or antigen-binding fragment thereof when formulated. Examples of aggregation reducing agents include one or more amino acids selected from the group consisting of methionine, arginine, lysine, aspartic acid, glycine, and glutamic acid. These amino acids may be added to the formulation to a concentration of about 0.5 mM to about 145 mM (e.g., 0.5 mM, 1 mM, 2 mM, 5 mM, 10 mM, 25 mM, 50 mM, 100 mM). The pharmaceutical compositions can also include a sugar (e.g., sucrose, trehalose, mannitol, sorbitol, or xylitol) and/or a tonicity modifier (e.g., sodium chloride, mannitol, or sorbitol) and/or a surfactant (e.g., polysorbate-20 or polysorbate-80).
Such compositions can be administered by a parenteral mode (e.g., intravenous, subcutaneous, intraperitoneal, or intramuscular injection). In one embodiment, the anti-BDCA2 antibody or antigen-binding fragment thereof compositions are administered subcutaneously. In one embodiment, the anti-BDCA2 antibody or antigen-binding fragment thereof compositions are administered intravenously. The phrases “parenteral administration” and “administered parenterally” as used herein mean modes of administration other than enteral and topical administration, usually by injection, and include, without limitation, intravenous, intramuscular, intraarterial, intrathecal, intracapsular, intraorbital, intracardiac, intradermal, intraperitoneal, transtracheal, subcutaneous, subcuticular, intraarticular, subcapsular, subarachnoid, intraspinal, epidural and intrasternal injection and infusion.
The composition can be formulated as a solution, microemulsion, dispersion, liposome, or other ordered structure suitable for stable storage at high concentration. Sterile injectable solutions can be prepared by incorporating an agent described herein in the required amount in an appropriate solvent with one or a combination of ingredients enumerated above, as required, followed by filtered sterilization. Generally, dispersions are prepared by incorporating an agent described herein into a sterile vehicle that contains a basic dispersion medium and the required other ingredients from those enumerated above. In the case of sterile powders for the preparation of sterile injectable solutions, the preferred methods of preparation are vacuum drying and freeze drying that yield a powder of an agent described herein plus any additional desired ingredient from a previously sterile-filtered solution thereof. The proper fluidity of a solution can be maintained, for example, by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersion and by the use of surfactants. Prolonged absorption of injectable compositions can be brought about by including in the composition an agent that delays absorption, for example, monostearate salts and gelatin.
In certain embodiments, the anti-BDCA2 antibody or antigen-binding fragment thereof may be prepared with a carrier that will protect the compound against rapid release, such as a controlled release formulation, including implants, and microencapsulated delivery systems. Biodegradable, biocompatible polymers can be used, such as ethylene vinyl acetate, polyanhydrides, polyglycolic acid, collagen, polyorthoesters, and polylactic acid. Many methods for the preparation of such formulations are patented or generally known. See, e.g., Sustained and Controlled Release Drug Delivery Systems, J. R. Robinson, ed., Marcel Dekker, Inc., New York (1978).
In one embodiment, the pharmaceutical formulation comprises an anti-BDCA2 antibody or antigen-binding fragment thereof (e.g., BIIB059) at a concentration of about 0.5 mg/mL to 300 mg/mL (e.g., 1 mg/mL, 5 mg/mL, 10 mg/mL, 25 mg/mL, 50 mg/mL, 75 mg/mL, 100 mg/mL, 125 mg/mL, 150 mg/mL, 175 mg/mL, 200 mg/mL, 250 mg/mL), formulated with sodium citrate, sodium chloride and optionally Tween-80 (0.01-0.1%, e.g., 0.03%, 0.05%, or 0.7%). The pH of the formulation may be between 5.5 and 7.5 (e.g., 5.6, 5.7, 5.8, 5.9, 6.0, 6.1, 6.2 6.3, 6.4 6.5, 6.6 6.7, 6.8, 6.9 7.0, 7.1, 7.3).
Administration
The anti-BDCA2 antibody or antigen-binding fragment thereof can be administered to a subject, e.g., a subject in need thereof, for example, a human subject, by a variety of methods. For many applications, the route of administration is one of: intravenous injection or infusion (IV), subcutaneous injection (SC), intraperitoneally (IP), or intramuscular injection. It is also possible to use intra-articular delivery. Other modes of parenteral administration can also be used. Examples of such modes include: intraarterial, intrathecal, intracapsular, intraorbital, intracardiac, intradermal, transtracheal, subcuticular, intraarticular, subcapsular, subarachnoid, intraspinal, and epidural and intrasternal injection. In some cases, administration can be oral.
The route and/or mode of administration of the antibody or antigen-binding fragment thereof can also be tailored for the individual case, e.g., by monitoring the subject, e.g., using tomographic imaging, e.g., to visualize a tumor.
The antibody or antigen-binding fragment thereof can be administered as a fixed dose, or in a mg/kg dose. The dose can also be chosen to reduce or avoid production of antibodies against the anti-BDCA2 antibody. Dosage regimens are adjusted to provide the desired response, e.g., a therapeutic response or a combinatorial therapeutic effect. Generally, doses of the anti-BDCA2 antibody (and optionally a second agent) can be used in order to provide a subject with the agent in bioavailable quantities. For example, doses in the range of 0.1-100 mg/kg, 0.5-100 mg/kg, 1 mg/kg-100 mg/kg, 0.5-20 mg/kg, 0.1-10 mg/kg, or 1-10 mg/kg can be administered. Other doses can also be used. In specific embodiments, a subject in need of treatment with an anti-BDCA2 antibody is administered the antibody at a dose 2 mg/kg, 4 mg/kg, 5 mg/kg, 10 mg/kg, 15 mg/kg, 20 mg/kg, 30 mg/kg, 35 mg/kg, or 40 mg/kg.
A composition may comprise about 1 mg/mL to 100 mg/ml or about 10 mg/mL to 100 mg/ml or about 50 to 250 mg/mL or about 100 to 150 mg/ml or about 100 to 250 mg/ml of anti-BDCA2 antibody or antigen-binding fragment thereof.
In certain embodiments, the anti-BDCA2 antibody or antigen-binding fragment thereof in a composition is predominantly in monomeric form, e.g., at least about 90%, 92%, 94%, 96%, 98%, 98.5% or 99% in monomeric form. Certain anti-BDCA2 antibody or antigen-binding fragment thereof compositions may comprise less than about 5, 4, 3, 2, 1, 0.5, 0.3 or 0.1% aggregates, as detected, e.g., by UV at A280 nm. Certain anti-BDCA2 antibody or antigen-binding fragment thereof compositions comprise less than about 5, 4, 3, 2, 1, 0.5, 0.3, 0.2 or 0.1% fragments, as detected, e.g., by UV at A280 nm.
Dosage unit form or “fixed dose” as used herein refers to physically discrete units suited as unitary dosages for the subjects to be treated; each unit contains a predetermined quantity of active compound calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier and optionally in association with the other agent. Single or multiple dosages may be given. Alternatively, or in addition, the antibody may be administered via continuous infusion.
An anti-BDCA2 antibody or antigen-binding fragment thereof dose can be administered, e.g., at a periodic interval over a period of time (a course of treatment) sufficient to encompass at least 2 doses, 3 doses, 5 doses, 10 doses, or more, e.g., once or twice daily, or about one to four times per week, or preferably weekly, biweekly (every two weeks), every three weeks, monthly, e.g., for between about 1 to 12 weeks, preferably between 2 to 8 weeks, more preferably between about 3 to 7 weeks, and even more preferably for about 4, 5, or 6 weeks. Factors that may influence the dosage and timing required to effectively treat a subject, include, e.g., the severity of the disease or disorder, formulation, route of delivery, previous treatments, the general health and/or age of the subject, and other diseases present. Moreover, treatment of a subject with a therapeutically effective amount of a compound can include a single treatment or, preferably, can include a series of treatments.
If a subject is at risk for developing an immunological disorder described herein, the antibody can be administered before the full onset of the immunological disorder, e.g., as a preventative measure. The duration of such preventative treatment can be a single dosage of the antibody or the treatment may continue (e.g., multiple dosages). For example, a subject at risk for the disorder or who has a predisposition for the disorder may be treated with the antibody for days, weeks, months, or even years so as to prevent the disorder from occurring or fulminating.
A pharmaceutical composition may include a “therapeutically effective amount” of an agent described herein. Such effective amounts can be determined based on the effect of the administered agent, or the combinatorial effect of agents if more than one agent is used. A therapeutically effective amount of an agent may also vary according to factors such as the disease state, age, sex, and weight of the individual, and the ability of the compound to elicit a desired response in the individual, e.g., amelioration of at least one disorder parameter or amelioration of at least one symptom of the disorder. A therapeutically effective amount is also one in which any toxic or detrimental effects of the composition are outweighed by the therapeutically beneficial effects.
In certain embodiments, the anti-BDCA2 antibody or antigen-binding fragment thereof is administered subcutaneously at a concentration of about 1 mg/mL to about 300 mg/mL (e.g., 1 mg/mL, 5 mg/mL, 10 mg/mL, 25 mg/mL, 50 mg/mL, 75 mg/mL, 100 mg/mL, 125 mg/mL, 150 mg/mL, 175 mg/mL, 200 mg/mL, 250 mg/mL). In one embodiment, the anti-BDCA2 antibody or antigen-binding fragment thereof is administered subcutaneously at a concentration of 50 mg/mL. In another embodiment, the anti-BDCA2 antibody or antigen-binding fragment thereof is administered intravenously at a concentration of about 1 mg/mL to about 300 mg/mL. In a particular embodiment, the anti-BDCA2 antibody or antigen-binding fragment thereof is administered intravenously at a concentration of 50 mg/mL.
Devices and Kits for Therapy
Pharmaceutical compositions that include the anti-BDCA2 antibody or antigen-binding fragment thereof can be administered with a medical device. The device can be designed with features such as portability, room temperature storage, and ease of use so that it can be used in emergency situations, e.g., by an untrained subject or by emergency personnel in the field, removed from medical facilities and other medical equipment. The device can include, e.g., one or more housings for storing pharmaceutical preparations that include anti-BDCA2 antibody or antigen-binding fragment thereof, and can be configured to deliver one or more unit doses of the antibody. The device can be further configured to administer a second agent, e.g., a chemo therapeutic agent, either as a single pharmaceutical composition that also includes the anti-BDCA2 antibody or antigen-binding fragment thereof or as two separate pharmaceutical compositions.
The pharmaceutical composition may be administered with a syringe. The pharmaceutical composition can also be administered with a needleless hypodermic injection device, such as the devices disclosed in U.S. Pat. Nos. 5,399,163; 5,383,851; 5,312,335; 5,064,413; 4,941,880; 4,790,824; or 4,596,556. Examples of well-known implants and modules include: U.S. Pat. No. 4,487,603, which discloses an implantable micro-infusion pump for dispensing medication at a controlled rate; U.S. Pat. No. 4,486,194, which discloses a therapeutic device for administering medicaments through the skin; U.S. Pat. No. 4,447,233, which discloses a medication infusion pump for delivering medication at a precise infusion rate; U.S. Pat. No. 4,447,224, which discloses a variable flow implantable infusion apparatus for continuous drug delivery; U.S. Pat. No. 4,439,196, which discloses an osmotic drug delivery system having multi-chamber compartments; and U.S. Pat. No. 4,475,196, which discloses an osmotic drug delivery system. Many other devices, implants, delivery systems, and modules are also known.
An anti-BDCA2 antibody or antigen-binding fragment thereof can be provided in a kit. In one embodiment, the kit includes (a) a container that contains a composition that includes anti-BDCA2 antibody, and optionally (b) informational material. The informational material can be descriptive, instructional, marketing or other material that relates to the methods described herein and/or the use of the agents for therapeutic benefit.
In an embodiment, the kit also includes a second agent for treating a disorder described herein (e.g., BTK inhibitor, an anti-malarial, glucocorticoid, NSAID, prednisone, hydroxychloroquine, amodiaquine, pyrimethamine, proguanil, sulfonamides, mefloquine, atovaquone, primaquine, artemisinin and derivatives, halofantrine, doxycycline, clindamycin, methotrexate, mycophenolate mofetil, azathioprine, cyclophosphamide, sulfasalazine or leflunomide). For example, the kit includes a first container that contains a composition that includes the anti-BDCA2 antibody, and a second container that includes the second agent.
The informational material of the kits is not limited in its form. In one embodiment, the informational material can include information about production of the compound, molecular weight of the compound, concentration, date of expiration, batch or production site information, and so forth. In one embodiment, the informational material relates to methods of administering the anti-BDCA2 antibody or antigen-binding fragment thereof, e.g., in a suitable dose, dosage form, or mode of administration (e.g., a dose, dosage form, or mode of administration described herein), to treat a subject who has had or who is at risk for an immunological disorder described herein. The information can be provided in a variety of formats, include printed text, computer readable material, video recording, or audio recording, or information that provides a link or address to substantive material, e.g., on the internet.
In addition to the antibody, the composition in the kit can include other ingredients, such as a solvent or buffer, a stabilizer, or a preservative. The antibody can be provided in any form, e.g., liquid, dried or lyophilized form, preferably substantially pure and/or sterile. When the agents are provided in a liquid solution, the liquid solution preferably is an aqueous solution. When the agents are provided as a dried form, reconstitution generally is by the addition of a suitable solvent. The solvent, e.g., sterile water or buffer, can optionally be provided in the kit.
The kit can include one or more containers for the composition or compositions containing the agents. In some embodiments, the kit contains separate containers, dividers or compartments for the composition and informational material. For example, the composition can be contained in a bottle, vial, or syringe, and the informational material can be contained in a plastic sleeve or packet. In other embodiments, the separate elements of the kit are contained within a single, undivided container. For example, the composition is contained in a bottle, vial or syringe that has attached thereto the informational material in the form of a label. In some embodiments, the kit includes a plurality (e.g., a pack) of individual containers, each containing one or more unit dosage forms (e.g., a dosage form described herein) of the agents. The containers can include a combination unit dosage, e.g., a unit that includes both the anti-BDCA2 antibody or antigen-binding fragment thereof and the second agent, e.g., in a desired ratio. For example, the kit includes a plurality of syringes, ampules, foil packets, blister packs, or medical devices, e.g., each containing a single combination unit dose. The containers of the kits can be air tight, waterproof (e.g., impermeable to changes in moisture or evaporation), and/or light-tight.
The kit optionally includes a device suitable for administration of the composition, e.g., a syringe or other suitable delivery device. The device can be provided pre-loaded with one or both of the agents or can be empty, but suitable for loading.
Diagnostic Uses
Anti-BDCA2 antibodies or antigen-binding fragments thereof can be used in a diagnostic method for detecting the presence of BDCA2, in vitro (e.g., a biological sample, such as tissue, biopsy) or in vivo (e.g., in vivo imaging in a subject). For example, human or effectively human anti-BDCA2 antibodies can be administered to a subject to detect BDCA2 within the subject. For example, the antibody can be labeled, e.g., with an MRI detectable label or a radiolabel. The subject can be evaluated using a means for detecting the detectable label. For example, the subject can be scanned to evaluate localization of the antibody within the subject. For example, the subject is imaged, e.g., by NMR or other tomographic means.
Examples of labels useful for diagnostic imaging include radiolabels such as 131I, 111In, 123I, 99mTc, 32P, 33P, 125I, 3H, 14C, and 188Rh, fluorescent labels such as fluorescein and rhodamine, nuclear magnetic resonance active labels, positron emitting isotopes detectable by a positron emission tomography (“PET”) scanner, chemiluminescers such as luciferin, and enzymatic markers such as peroxidase or phosphatase. Short-range radiation emitters, such as isotopes detectable by short-range detector probes, can also be employed. The protein ligand can be labeled with such reagents using known techniques. For example, see Wensel and Meares (1983) Radioimmunoimaging and Radioimmunotherapy, Elsevier, New York for techniques relating to the radiolabeling of antibodies and Colcher et al. (1986) Meth. Enzymol. 121: 802-816.
The subject can be “imaged” in vivo using known techniques such as radionuclear scanning using e.g., a gamma camera or emission tomography. See e.g., A. R. Bradwell et al., “Developments in Antibody Imaging”, Monoclonal Antibodies for Cancer Detection and Therapy, R. W. Baldwin et al., (eds.), pp 65-85 (Academic Press 1985). Alternatively, a positron emission transaxial tomography scanner, such as designated Pet VI located at Brookhaven National Laboratory, can be used where the radiolabel emits positrons (e.g., 11C, 18F, 15O, and 13N).
Magnetic Resonance Imaging (MRI) uses NMR to visualize internal features of living subject, and is useful for prognosis, diagnosis, treatment, and surgery. MRI can be used without radioactive tracer compounds for obvious benefit. Some MRI techniques are summarized in EP0 502 814 A. Generally, the differences related to relaxation time constants T1 and T2 of water protons in different environments is used to generate an image. However, these differences can be insufficient to provide sharp high resolution images.
The differences in these relaxation time constants can be enhanced by contrast agents. Examples of such contrast agents include a number of magnetic agents, paramagnetic agents (which primarily alter T1) and ferromagnetic or superparamagnetic agents (which primarily alter T2 response). Chelates (e.g., EDTA, DTPA and NTA chelates) can be used to attach (and reduce toxicity) of some paramagnetic substances (e.g., Fe3+, Mn2+, Gd3+). Other agents can be in the form of particles, e.g., less than 10 μm to about 10 nm in diameter). Particles can have ferromagnetic, anti-ferromagnetic or superparamagnetic properties. Particles can include, e.g., magnetite (Fe3O4), γ-Fe2O3, ferrites, and other magnetic mineral compounds of transition elements. Magnetic particles may include one or more magnetic crystals with and without nonmagnetic material. The nonmagnetic material can include synthetic or natural polymers (such as sepharose, dextran, dextrin, starch and the like).
The anti-BDCA2 antibodies or antigen-binding fragments thereof can also be labeled with an indicating group containing the NMR-active 19F atom, or a plurality of such atoms inasmuch as (i) substantially all of naturally abundant fluorine atoms are the 19F isotope and, thus, substantially all fluorine-containing compounds are NMR-active; (ii) many chemically active polyfluorinated compounds such as trifluoracetic anhydride are commercially available at relatively low cost, and (iii) many fluorinated compounds have been found medically acceptable for use in humans such as the perfluorinated polyethers utilized to carry oxygen as hemoglobin replacements. After permitting such time for incubation, a whole body MRI is carried out using an apparatus such as one of those described by Pykett (1982) Scientific American, 246:78-88 to locate and image BDCA2 distribution.
In another aspect, the disclosure provides a method for detecting the presence of BDCA2 in a sample in vitro (e.g., a biological sample, such as serum, plasma, tissue, biopsy). The subject method can be used to diagnose a disorder, e.g., an autoimmune disorder (e.g., SLE) or a to detect levels of pDCs in a sample. The method includes: (i) contacting the sample or a control sample with the anti-BDCA2 antibody; and (ii) evaluating the sample for the presence of BDCA2, e.g., by detecting formation of a complex between the anti-BDCA2 antibody and BDCA2, or by detecting the presence of the antibody or BDCA2. For example, the antibody can be immobilized, e.g., on a support, and retention of the antigen on the support is detected, and/or vice versa. The antibody used may be labeled e.g., with a fluorophore. A control sample can be included. The positive control can be a sample known to have the disease or disorder being assessed, and a negative control can be a sample from a subject who does not have the disease or disorder being assessed. A statistically significant change in the formation of the complex in the sample relative to the control sample can be indicative of the presence of BDCA2 in the sample. Generally, an anti-BDCA2 antibody can be used in applications that include fluorescence polarization, microscopy, ELISA, centrifugation, chromatography, and cell sorting (e.g., fluorescence activated cell sorting). In certain embodiments, the anti-BDCA2 antibody is BIIB059 or Dendritics clone 124B3.13. In some embodiments, the method further involves immunostaining a tissue sample with an anti-CD123 antibody. The tissue sample can be, e.g., skin biopsies from human patients with autoimmune conditions, e.g., SLE.
The following are examples of the practice of the invention. They are not to be construed as limiting the scope of the invention in any way.
EXAMPLES
The following examples are provided to better illustrate the claimed invention and are not to be interpreted as limiting the scope of the invention. To the extent that specific materials are mentioned, it is merely for purposes of illustration and is not intended to limit the invention. One skilled in the art can develop equivalent means or reactants without the exercise of inventive capacity and without departing from the scope of the invention.
Example 1: Cloning of the Heavy and Light Chains of Murine Anti-BDCA2 Antibody
The 24F4 murine hybridoma (IgG1, kappa) was derived from a Balb/c mouse immunized by Gene Gun with the plasmid pEAG2456, a mammalian expression vector which co-expresses full-length human BDCA2 and FcεRIγcDNAs (see Example 17).
Total cellular RNA from the 24F4 murine hybridoma cells was prepared using a Qiagen RNeasy mini kit following the manufacturer's recommended protocol. cDNAs encoding the variable regions of the heavy and light chains were cloned by RT-PCR from total cellular RNA using the GE Healthcare First Strand cDNA Synthesis kit following the manufacturer's recommended protocol using random hexamers for priming.
For PCR amplification of the murine immunoglobulin variable domains with intact signal sequences, a cocktail of degenerate forward primers hybridizing to multiple murine immunoglobulin gene family signal sequences and a single back primer specific for 5′ end of the murine constant domain as described in Current Protocols in Immunology (Wiley and Sons, 1999) were used. The 24F4 heavy chain variable domain was amplified with the following primers: 5′ ACT AGT CGA CAT GRA CTT TGG GYT CAG CTT GRT TT 3′ (R=A/G and Y=C/T) (SEQ ID NO:25) and 5′ AGG TCT AGA AYC TCC ACA CAC AGG RRC CAG TGG ATA GAC 3′ (R=A/G and Y=C/T) (SEQ ID NO:26). The 24F4 light chain variable domain with its signal sequence was amplified with the following primers: 5′ ACT AGT CGA CAT GGA GWC AGA CAC ACT CCT GYT ATG GGT 3′ (W=A/T and Y=C/T) (SEQ ID NO: 27) and 5′ GCG TCT AGA ACT GGA TGG TGG GAG ATG GA 3′ (SEQ ID NO:28).
The PCR products were gel-purified using a Qiagen Qiaquick gel extraction kit following the manufacturer's recommended protocol. Purified PCR products were subcloned into Invitrogen's pCR2.1TOPO vector using their TOPO cloning kit following the manufacturer's recommended protocol. Inserts from multiple independent subclones were sequenced to establish a consensus sequence (from heavy chain clone designated pYL647 and light chain clone pYL651).
The variation in the sequences amongst the clones was consistent with the primers' positions of degeneracy. BLAST analysis of the variable domain sequences confirmed their immunoglobulin identity. The deduced mature light and heavy chain N-terminal sequences match those of the authentic 24F4 chains derived from Edman degradation data. Deduced intact masses from hypothetical sequences assembled by adding deduced constant domain sequences from cloned Balb/c IgG1 heavy chain and kappa light chain cDNAs to the deduced mature variable domain sequences were consistent with those of the purified hybridoma-derived 24F4 determined by mass spectroscopy.
The murine 24F4 heavy chain variable domain (VH) is a member of murine subgroup III(D). The sequence of the murine 24F4 mature heavy chain variable domain with CDR H1, CDR H2, and CDR H3 underlined in that order is shown below:







(SEQ ID NO: 29)


  1 DVKLVESGGG LVKPGGSLKL SCAASGFTFS TYTMSWVRQT


 


    PEKRLEWVAT


 


 51 ISPGDSFGYY YPDSVQGRFT ISRDNAKNTL FLQMSSLKSE


 


    DTAMYYCTRD


 


101 IYYNYGAWFA YWGQGTLVTV SA






The murine 24F4 light chain variable domain (VL) is a member of murine kappa subgroup III. The sequence of the murine 24F4 mature light chain variable domain with CDR L1, CDR L2, and CDR L3 underlined in that order is shown below:








(SEQ ID NO: 30)


  1 DIVLTQSPAS LAVSLGQRAT ISCKASQSVD YDGDSYMNWY


 


    QQKPGQPPKL


 


 51 LIYAASTLES GVPARFSGSG SGTDFTLNIH PVEEEDAATY


 


    YCQQCNEDPR


 


101 TFGGGTKLEI K






An unpaired cysteine is present at residue 95 in CDRL3 in the murine 24F4 VL sequence above (in Kabat nomenclature this Cys is residue 91).

Example 2. Chimerization of the Murine 24F4 Antibody
cDNAs encoding the murine 24F4 variable domains were used to construct vectors for expression of murine-human chimeras (ch24F4) in which the mu24F4 variable regions were linked to human IgG1 and kappa constant regions.
The variable domains were first engineered by PCR to add a 5′ Kozak sequence and to introduce human sequences and new restriction sites at the FR4/constant domain junctions for fusion to human immunoglobulin constant domains. The variable region cDNA sequences in the resultant plasmids were confirmed by DNA sequencing. The heavy chain variable domain in plasmid pYL647 was used as template for PCR with the primers 5′ GAT CCG CGG CCG CAC CAT GGA CTT TGG GTT CAG CTT G 3′ (SEQ ID NO:31) (adds NotI site and Kozak sequence) and 5′ GAT GGG CCC TTG GTG GAA GCT GCA GAG ACA GTG ACC AGA G 3′ (SEQ ID NO:32) (adds human IgG1 CH1 sequences at FR4/constant domain junction and an ApaI site), amplifying a 0.45 kb fragment that was purified and subcloned into the Invitrogen pCRBluntIITOPO cloning vector, generating pYL668. For construction of the heavy chain chimera, the 0.45 kb NotI-ApaI fragment from the 24F4 heavy chain variable domain construct pYL668 and the 0.98 kb ApaI-BamHI fragment from pEAG1325 (a plasmid containing a sequence-confirmed huIgG1 heavy chain constant domain cDNA (with the IgG1 C-terminal lysine residue genetically removed) were subcloned into the vector backbone of the expression vector pV90 (in which heterologous gene expression is controlled by a CMV-IE promoter and a human growth hormone polyadenylation signal and which carries a dhfr selectable marker, see U.S. Pat. No. 7,494,805), to produce the expression vector pYL672. The heavy chain cDNA sequence in the resultant plasmid pYL672 was confirmed by DNA sequencing. The deduced mature ch24F4-huIgG1 heavy chain protein sequence encoded by pYL672 is shown below:







(SEQ ID NO: 33)


  1 DVKLVESGGG LVKPGGSLKL SCAASGFTFS TYTMSWVRQT


 


    PEKRLEWVAT


 


 51 ISPGDSFGYY YPDSVQGRFT ISRDNAKNTL FLQMSSLKSE


 


    DTAMYYCTRD


 


101 IYYNYGAWFA YWGQGTLVTV SAASTKGPSV FPLAPSSKST


 


    SGGTAALGCL


 


151 VKDYFPEPVT VSWNSGALTS GVHTFPAVLQ SSGLYSLSSV


 


    VTVPSSSLGT


 


201 QTYICNVNHK PSNTKVDKKV EPKSCDKTHT CPPCPAPELL


 


    GGPSVFLFPP


 


251 KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH


 


    NAKTKPREEQ


 


301 YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT


 


    ISKAKGQPRE


 


351 PQVYTLPPSR DELTKNQVSL TCLVKGFYPS DIAVEWESNG


 


    QPENNYKTTP


 


401 PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH


 


    YTQKSLSLSP


 


451 G






An aglycosyl low effector function form of ch24F4 was also constructed by subcloning the 0.45 kb NotI-ApaI fragment from the 24F4 heavy chain variable domain construct pYL668 and the 0.98 kb ApaI-BamHI fragment from pEAG2412 (a plasmid containing a sequence-confirmed S228P/N299Q huIgG4/IgG1 hybrid heavy chain constant domain cDNA with the IgG1 C-terminal lysine residue genetically removed) were subcloned into the vector backbone of expression vector pV90, generating plasmid pYL670. The heavy chain cDNA sequence in the resultant plasmid pYL670 was confirmed by DNA sequencing. The deduced mature agly ch24F4-huIgG4/G1 hybrid heavy chain protein sequence encoded by pYL670 is shown below:







(SEQ ID NO: 34)


  1 DVKLVESGGG LVKPGGSLKL SCAASGFTFS TYTMSWVRQT


 


    PEKRLEWVAT


 


 51 ISPGDSFGYY YPDSVQGRFT ISRDNAKNTL FLQMSSLKSE


 


    DTAMYYCTRD


 


101 IYYNYGAWFA YWGQGTLVTV SAASTKGPSV FPLAPCSRST


 


    SESTAALGCL


 


151 VKDYFPEPVT VSWNSGALTS GVHTFPAVLQ SSGLYSLSSV


 


    VTVPSSSLGT


 


201 KTYTCNVDHK PSNTKVDKRV ESKYGPPCPP CPAPEFLGGP


 


    SVFLFPPKPK


 


251 DTLMISRTPE VTCVVVDVSQ EDPEVQFNWY VDGVEVHNAK


 


    TKPREEQFQS


 


301 TYRVVSVLTV LHQDWLNGKE YKCKVSNKGL PSSIEKTISK


 


    AKGQPREPQV


 


351 YTLPPSRDEL TKNQVSLTCL VKGFYPSDIA VEWESNGQPE


 


    NNYKTTPPVL


 


401 DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ


 


    KSLSLSPG






The kappa light chain variable domain in plasmid pYL651 was used as template for PCR with the primers 5′ GAT CCG CGG CCG CCA CCA TGG AGA CAG ACA CAC TCC TG 3′ (SEQ ID NO:35) (adds a 5′ NotI site and Kozak sequence) and 5′ CCA CCG TAC GTT TGA TTT CCA GCT TGG TGC 3′ (SEQ ID NO:36) (adds human kappa constant domain sequences at FR4/constant domain junction and a 3′ BsiWI site), amplifying a 0.4 kb fragment that was purified and subcloned into the Invitrogen pCRBluntIITOPO cloning vector, generating pYL669. The variable region cDNA sequences in plasmid pYL669 were confirmed by DNA sequencing. For construction of the light chain chimera, the 0.4 kb NotI-BsiWI light chain variable domain fragment from pYL669 and the 0.34 kb BsiWI-BamHI fragment from the plasmid pEAG1572 (containing a sequence-confirmed human kappa light chain constant domain cDNA) were subcloned into the vector backbone of pV100 (in which heterologous gene expression is controlled by a CMV-IE promoter and a human growth hormone polyadenylation signal and which carries a neomycin selectable marker), to produce the expression vector pYL671. The light chain cDNA sequence in the resultant plasmid pYL671 was confirmed by DNA sequencing. The deduced mature ch24F4-human kappa light chain protein sequence encoded by pYL671 is shown below:







(SEQ ID NO: 37)


  1 DIVLTQSPAS LAVSLGQRAT ISCKASQSVD YDGDSYMNWY


 


    QQKPGQPPKL


 


 51 LIYAASTLES GVPARFSGSG SGTDFTLNIH PVEEEDAATY


 


    YCQQCNEDPR


 


101 TFGGGTKLEI KRTVAAPSVF IFPPSDEQLK SGTASVVCLL


 


    NNFYPREAKV


 


151 QWKVDNALQS GNSQESVTEQ DSKDSTYSLS STLTLSKADY


 


    EKHKVYACEV


 


201 THQGLSSPVT KSFNRGEC






Expression vectors (ch24F4 heavy chain vectors pYL670 or pYL672 and ch24F4 light chain vector pYL671) were co-transfected into 293-EBNA cells and transfected cells were tested for antibody secretion and specificity (empty vector- and a molecularly cloned irrelevant mAb vector-transfected cells served as controls). Western blot analysis (developed with anti-human heavy and light chain antibodies) of conditioned medium indicated that ch24F4-transfected cells synthesized and efficiently secreted heavy and light chains. Direct FACS binding to surface human BDCA2 confirmed the specificity of ch24F4. The EC50 binding of both variants of ch24F4 was equivalent to that of the murine 24F4 mAb by direct binding to surface expressed human BDCA2 by dilution titration FACS assay. Stable CHO cell lines secreting ch24F4-huIgG1, kappa mAb and agly ch24F4-huIgG4/G1 hybrid kappa mAb were produced by co-transfection with pYL672/pYL671 and pYL670/pYL671, respectively.
Example 3. Removal of an Unpaired Cysteine Residue in CDRL3 of the Chimeric 24F4 Antibody
As unpaired cysteines in an exposed CDR can produce product heterogeneity or instability, ch24F4 variants C95S and C95T were constructed by site-directed mutagenesis using the ch24F4 light chain expression vector plasmid pYL671 as template.
Site-directed mutagenesis was performed using Agilent's QuikChange II mutagenesis kit following the manufacturer's recommended protocol. The C95S variant was constructed using the mutagenic primer 5′ GCA ACC TAT TAC TGT CAA CAA AGT AAT GAG GAT CCT CGG AC 3′ (SEQ ID NO: 38) and its reverse complement, which introduced a new HincII site, producing plasmid pEAG2678. The C95T variant was constructed using the mutagenic primer 5′ CAA CCT ATT ACT GTC AGC AAA CTA ATG AAG ATC CTC GGA CGT TCG 3′ (SEQ ID NO: 39) and its reverse complement, which removed a BamHI site, producing plasmid pEAG2679. Mutated plasmids were identified by screening for the introduced restriction site changes. The full-length light chain cDNA sequences in the resultant plasmids were confirmed by DNA sequencing. Wildtype ch24F4 and the C95S and C95T variant mAbs were expressed transiently in 293E cells by co-transfection of pYL672 and pYL671, pEAG2678 or pEAG2679. Conditioned medium was harvested at 2 days post-transfection. Titers (assayed by Octet on anti-human Fc tips) of both variants were similar to that of wildtype ch24F4, and Western blots of nonreducing SDS-PAGE indicated no gross aggregation or obvious clipping relative to wildtype ch24F4 mAb. Direct binding by FACS on surface BDCA2 indicated that while the apparent EC50 for binding by the C95S variant was equivalent to that of wildtype ch24F4, the EC50 binding of the C95T variant was reduced by several-fold. Conditioned medium containing ch24F4 and the C95 variant mAbs was assayed by Octet for binding to human BDCA2 ectodomain. Antibodies from conditioned medium from transiently transfected cells were bound to anti-human Fc tips, then monomeric huBDCA2 was flowed over the Octet tips, to examine binding and dissociation. Octet binding and dissociation kinetics for wildtype ch24F4 and the C95S variant were equivalent, while the off-rate of the C95T variant was faster than that of wildtype ch24F4. Based upon these results, C95S was incorporated into the humanized 24F4 light chain CDRL3.
Example 4. Exemplary Humanized 24F4 Heavy and Light Chains
Examples of seven humanized (hu) 24F4 heavy chains (huIGHV3-21*01 framework/24F4 VH CDRs) and their corresponding DNA sequences are shown below. CDRs 1, 2, and 3 in each heavy chain are underlined in that order. Framework backmutations are shown in lowercase bold font. Changes to CDR residues from murine 24F4 are shown by shading within the CDR sequences. CDR1 of the variable heavy chain (CDR H1) is defined according to the Chothia definition, which is 5 amino acids longer than the Kabat definition; the italicized residues in CDR H1 identify the additional 5 amino acids (i.e., GFTFS (SEQ ID NO:12)) that form the Chothia CDR H1. The N-terminal most amino acid (i.e., glutamic acid in versions H0, H1, H2, and H3 and aspartic acid in versions H4, H5, and H6) of the variable heavy chain domain may contact antigen directly and affect binding affinity. The buried residue at Kabat position 49 may affect the conformation of CDR2 of the heavy chain (serine in versions H0, H1, H2, and H3; and alanine in versions H4, H5 and H6). The residue at Kabat position 93 may have an effect on heavy-light chain pairing (alanine in versions H0, H1, H2, and H3; and threonine in versions H4, H5 and H6. The amino acid residues in the CDR H1, H2, and H3 regions that differ from the murine 24F4 CDR H1, H2, and H3 are shaded.








Version H0



(SEQ ID NO: 40)










KNSLYLQMNSLRAEDTAVYYCARDIYYNYGAWFAYWGQGTLVTVSS


 


(SEQ ID NO: 41)



GAAGTGCAGCTGGTGGAGTCTGGGGGAGGCTTAGTGAAGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCT



 


GGATTCACTTTCAGTACCTATACCATGTCTTGGGTTCGCCAAGCACCGGGCAAGGGACTGGAGTGGGTCTCTGCT


 


ATTAGTGGTAGCGGAGGTAGTACATACTATGCAGACAGTGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCC


 


AAGAACAGTCTGTACCTGCAAATGAACAGTCTGAGGGCAGAGGACACAGCCGTGTATTACTGTGCTCGAGATATC


 


TACTATAATTACGGAGCCTGGTTTGCTTACTGGGGCCAAGGGACTCTGGTCACTGTCTCTAGC 


 


(pYL742)


Version H1


(SEQ ID NO: 42)










KNSLYLQMNSLRAEDTAVYYCARDIYYNYGAWFAYWGQGTLVTVSS 


 


(SEQ ID NO: 43)



GAAGTGCAGCTGGTGGAGTCTGGGGGAGGCTTAGTGAAGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCT



 


GGATTCACTTTCAGTACCTATACCATGTCTTGGGTTCGCCAAGCACCGGGCAAGGGACTGGAGTGGGTCTCTACC


 


ATTAGTCCAGGAGACAGTTTCGGATACTATCCAGACAGTGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCC


 


AAGAACAGTCTGTACCTGCAAATGAACAGTCTGAGGGCAGAGGACACAGCCGTGTATTACTGTGCTCGAGATATT


 


TACTATAATTACGGAGCCTGGTTTGCTTACTGGGGCCAAGGGACTCTGGTCACTGTCTCTAGC 


 


(pYL743)


Version H2


(SEQ ID NO: 44)










AKNSLYLQMNSLRAEDTAVYYCARDIYYNYGAWFAYWGQGTLVTVSS


 


(SEQ ID NO: 45)



GAAGTGCAGCTGGTGGAGTCTGGGGGAGGCTTAGTGAAGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCT



 


GGATTCACTTTCAGTACCTATACCATGTCTTGGGTTCGCCAAGCACCGGGCAAGGGACTGGAGTGGGTCTCTACC


 


ATTAGTCCAGGAGACAGTAGCACTATCTACTATGCAGACAGTGTGAAGGGCCGATTCACCATCTCCAGAGACAAT


 


GCCAAGAACAGTCTGTACCTGCAAATGAACAGTCTGAGGGCAGAGGACACAGCCGTGTATTACTGTGCCCGAGAT


 


ATTTACTATAATTACGGAGCCTGGTTTGCTTACTGGGGCCAAGGGACTCTGGTCACTGTCTCTAGC 


 


(pYL744)


Version H3


(SEQ ID NO: 46)



EVQLVESGGGLVKPGGSLRLSCAASGFTFSTYTMSWVRQAPGKGLEWVSTISPGDSFGYYYPDSVQGRFTISRDN



 


AKNSLYLQMNSLRAEDTAVYYCARDIYYNYGAWFAYWGQGTLVTVSS


 


(SEQ ID NO: 47)



GAAGTGCAGCTGGTGGAGTCTGGGGGAGGCTTAGTGAAGCCTGGAGGGTCCCTGAGACTCTCCTGCGCAGCCTCT



 


GGATTCACTTTCAGTACCTATACCATGTCTTGGGTTCGCCAAGCACCGGGCAAGGGACTGGAGTGGGTCTCTACC


 


ATTAGTCCAGGAGACAGTTTCGGCTACTACTATCCAGACAGTGTGCAGGGCCGATTCACCATCTCCAGAGACAAT


 


GCCAAGAACAGTCTGTACCTGCAAATGAACAGTCTGAGGGCAGAGGACACAGCCGTGTATTACTGTGCCCGAGAT


 


ATTTACTATAATTACGGAGCCTGGTTTGCTTACTGGGGCCAAGGGACTCTGGTCACTGTCTCTAGC 


 


(pYL745)


Version H4


(SEQ ID NO: 24)



dVQLVESGGGLVKPGGSLRLSCAASGFTFSTYTMSWVRQAPGKGLEWVaTISPGDSFGYYYPDSVQGRFTISRDN



 


AKNSLYLQMNSLRAEDTAVYYCtRDIYYNYGAWFAYWGQGTLVTVSS


 


(SEQ ID NO: 48)



GACGTCCAGCTGGTGGAGTCTGGGGGAGGCTTAGTGAAGCCTGGAGGGTCCCTGAGACTCTCCTGCGCAGCCTCT



 


GGATTCACTTTCAGTACCTATACCATGTCTTGGGTTCGCCAAGCACCGGGCAAGGGACTGGAGTGGGTCGCAACC


 


ATTAGTCCAGGAGACAGTTTCGGCTACTACTATCCAGACAGTGTCCAGGGCCGATTCACCATCTCCAGAGACAAT


 


GCCAAGAACAGTCTGTACCTGCAAATGAACAGTCTGAGGGCAGAGGACACAGCCGTGTATTACTGTACCCGAGAT


 


ATTTACTATAATTACGGAGCCTGGTTTGCTTACTGGGGCCAAGGGACTCTGGTCACTGTCTCTAGC 


 


(pYL746)


Version H5


(SEQ ID NO: 49)



dVQLVqSGGGLVKPGGSLRLSCAASGFTFSTYTMSWVRQAPGKGLEWVaTISPGDSFGYYYPDSVQGRFTISRDN



 


AKNSLYLQMNrLRAEDTAVYYCtRDIYYNYGAWFAYWGrGTLVTVSS


 


(SEQ ID NO: 50)



GACGTCCAGCTGGTGCAGTCTGGGGGAGGCTTAGTGAAGCCTGGAGGGTCCCTGAGACTCTCCTGCGCAGCCTCT



 


GGATTCACTTTCAGTACCTATACCATGTCTTGGGTTCGCCAAGCACCGGGCAAGGGACTGGAGTGGGTCGCAACC


 


ATTAGTCCAGGAGACAGTTTCGGCTACTACTATCCAGACAGTGTCCAGGGCCGATTCACCATCTCCAGAGACAAT


 


GCCAAGAACAGTCTGTACCTGCAAATGAACAGGCTGAGGGCAGAGGACACAGCCGTGTATTACTGTACCCGAGAT


 


ATTTACTATAATTACGGAGCCTGGTTTGCTTACTGGGGCAGAGGGACTCTGGTCACTGTCTCTAGC


 


(pYL747)


Version H6


(SEQ ID NO: 52)










AKNSLYLQMNSLRAEDTAVYYCtRDIYYNYGAWFAYWGQGTLVTVSS


 


(SEQ ID NO: 53)



GACGTCCAGCTGGTGGAGTCTGGGGGAGGCTTAGTGAAGCCTGGAGGGTCCCTGAGACTCTCCTGCGCAGCCTCT



 


GGATTCACTTTCAGTACCTATACCATGTCTTGGGTTCGCCAAGCACCGGGCAAGGGACTGGAGTGGGTCGCAACC


 


ATTAGTGGCGGAAATAACTACGGCTACTCCTATCCAGACAGTGTCAAGGGCCGATTCACCATCTCTAGAGACAAT


 


GCCAAGAACAGTCTGTACCTGCAAATGAACTCCCTGAGGGCAGAGGACACAGCCGTGTATTACTGTACCCGAGAT


 


ATTTACTATAATTACGGAGCCTGGTTTGCTTACTGGGGCCAGGGGACTCTGGTCACTGTCTCTAGC 


 


(pYL748)






An alignment of the amino acid sequences of versions H0 to H6 is shown below:









H0 EVQLVESGGGLVKPGGSLRLSCAASGFTESTYTMSWVRQAPGKGLEWVSAIS-GSGGSTY



 


H1 EVQLVESGGGLVKPGGSLRLSCAASGFTESTYTMSWVRQAPGKGLEWVSTISPGDSFG-Y


 


H2 EVQLVESGGGLVKPGGSLRLSCAASGFTESTYTMSWVRQAPGKGLEWVSTISPGDSSTIY


 


H3 EVQLVESGGGLVKPGGSLRLSCAASGFTESTYTMSWVRQAPGKGLEWVSTISPGDSFGYY


 


H4 DVQLVESGGGLVKPGGSLRLSCAASGFTFSTYTMSWVRQAPGKGLEWVATISPGDSFGYY


 


H5 DVQLVQSGGGLVKPGGSLRLSCAASGFTFSTYTMSWVRQAPGKGLEWVATISPGDSFGYY


 


H6 DVQLVESGGGLVKPGGSLRLSCAASGFTFSTYTMSWVRQAPGKGLEWVATISGGNNYGYS


   :****:******************************************::** *..


(SEQ ID NO: 40)



H0 YADSVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCARDIYYNYGAWFAYWGQGTLVTVSS



 


(SEQ ID NO: 42)



H1 YPDSVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCARDIYYNYGAWFAYWGQGTLVTVSS



 


(SEQ ID NO: 44)



H2 YADSVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCARDIYYNYGAWFAYWGQGTLVTVSS



 


(SEQ ID NO: 46)



H3 YPDSVQGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCARDIYYNYGAWFAYWGQGTLVTVSS



 


(SEQ ID NO: 24)



H4 YPDSVQGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCTRDIYYNYGAWFAYWGQGTLVTVSS



 


(SEQ ID NO: 49)



H5 YPDSVQGRFTISRDNAKNSLYLQMNRLRAEDTAVYYCTRDIYYNYGAWFAYWGRGTLVTVSS



 


(SEQ ID NO: 52)



H6 YPDSVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCTRDIYYNYGAWFAYWGQGTLVTVSS



   *:***:******************* ***********:***************:********






Examples of three humanized 24F4 light chains (huIGKV1-13*02 framework/24F4 VL CDRs) and their corresponding DNA sequences are shown below. CDRs 1, 2, and 3 in each light chain are underlined in that order. Ser91 (according to Kabat numbering), which has been substituted for Cys91 in all light chains, is highlighted. The N-terminal most amino acid (i.e., alanine in version L0 and aspartic acid in versions L1 and L2) of the variable light chain domain may contact antigen directly and affect binding affinity. Framework backmutations are shown in lowercase bold font. The first version (L0) contains the fewest backmutations and the third version (L2) contains the most backmutations (i.e., the least “humanized”).








Version L0



(SEQ ID NO: 54)



AIQLTQSPSSLSASVGDRVTITCKASQSVDYDGDSYMNWYQQKPGKAPKLLIYAASTLESGVPSRFSGSGSGTDF



 








(SEQ ID NO: 55)



GCTATTCAGCTGACCCAATCTCCATCCTCTTTGTCCGCCTCTGTGGGGGACAGGGTCACCATCACCTGCAAGGCC



 


AGCCAAAGTGTTGATTATGATGGTGATAGTTATATGAACTGGTATCAACAGAAACCAGGGAAGGCTCCCAAACTC


 


CTCATCTACGCTGCATCCACTCTCGAGTCTGGGGTCCCATCCAGGTTTAGTGGCAGTGGGTCTGGGACAGACTTC


 


ACCCTCACAATCAGCTCACTCCAGCCAGAGGATTTCGCAACCTATTACTGTCAGCAAAGCAACGAGGATCCTCGG


 


ACGTTCGGTCAGGGCACCAAAGTGGAAATCAAG (pYL729)


 


Version L1


(SEQ ID NO: 56)



dIQLTQSPSSLSASVGDRVTITCKASQSVDYDGDSYMNWYQQKPGKAPKLLIYAASTLESGVPSRFSGSGSGTDF



 








(SEQ ID NO: 57)



GACATTCAGCTGACCCAATCTCCATCCTCTTTGTCCGCCTCTGTGGGGGACAGGGTCACCATCACCTGCAAGGCC



 


AGCCAAAGTGTTGATTATGATGGTGATAGTTATATGAACTGGTATCAACAGAAACCAGGGAAGGCTCCCAAACTC


 


CTCATCTACGCTGCATCCACTCTCGAGTCTGGGGTCCCATCCAGGTTTAGTGGCAGTGGGTCTGGGACAGACTTC


 


ACCCTCACAATCAGCTCACTCCAGCCAGAGGATTTCGCAACCTATTACTGTCAGCAAAGCAACGAGGATCCTCGG


 


ACGTTCGGTCAGGGCACCAAAGTGGAAATCAAG (pYL730)


 


Version L2


(SEQ ID NO: 58)



dIQLTQSPSSLSvSVGDRaTIsCKASQSVDYDGDSYMNWYQQKPGKAPKLLIYAASTLESGVPSRFSGSGSGTDF



 








(SEQ ID NO: 59)



GACATTCAGCTGACCCAATCTCCATCCTCTTTGTCCGTCTCTGTGGGGGACAGGGCAACCATCTCCTGCAAGGCC



 


AGCCAAAGTGTTGATTATGATGGTGATAGTTATATGAACTGGTATCAACAGAAACCAGGGAAGGCTCCCAAACTC


 


CTCATCTACGCTGCATCCACTCTTGAGTCTGGGGTCCCATCCAGGTTTAGTGGCAGTGGGTCTGGGACAGACTTC


 


ACCCTCACAATCAGCTCAGTGCAGCCAGAGGATTTCGCAACCTATTACTGTCAGCAAAGCAACGAGGATCCTCGG


 


ACGTTCGGTCAGGGCACCAAAGTGGAAATCAAG (pYL731)






An alignment of the amino acid sequences of versions L0 to L2 is shown below:











L0
AIQLTQSPSSLSASVGDRVTITCKASQSVDYDGDSYMNWYQQKPGKAPKLLIYAASTLES




 


L1
DIQLTQSPSSLSASVGDRVTITCKASQSVDYDGDSYMNWYQQKPGKAPKLLIYAASTLES



 


L2
DIQLTQSPSSLSVSVGDRATISCKASQSVDYDGDSYMNWYQQKPGKAPKLLIYAASTLES




.***********.*****.**:**************************************



 


L0
GVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSNEDPRTFGQGTKVEIK
(SEQ ID NO: 54)


 


L1
GVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSNEDPRTFGQGTKVEIK
(SEQ ID NO: 56)


 


L2
GVPSRFSGSGSGTDFTLTISSVQPEDFATYYCQQSNEDPRTFGQGTKVEIK
(SEQ ID NO: 58)



*********************:*****************************








The humanized VH and VL amino acid sequences above do not contain any potential N-linked glycosylation sites or Asn-Gly deamidation sites. The methionines in both the VH and VL domains are observed in germline sequences, and are not surface exposed, so the risk of methionine oxidation appears to be minimal.
Solubility of proteins can correlate with their pI. The pI's of the designed constructs were calculated using pK's of amino acids in Bjellqvist et al. (Electrophoresis, 14:1023-31 (1993); Electrophoresis, 15:529-39 (1994)). The values shown below were calculated using human IgG1 heavy chains. Each of the humanized antibodies has a pI significantly above 7 and is therefore expected to have a significant positive charge at neutral pH. Each entry in the table is the calculated pI value of the full combined antibody, with the net charge in parentheses.














Molecule
Calculated pI (net charge)









Chimeric 24F4
6.94 (−2)



Humanized H4L1
7.26 (0) 










Example 5. Binding of Hx/L1 to BDCA2
All 21 possible variants of hu24F4 heavy and light chains (described in Example 4) and ch24F4 were expressed transiently in 293E cells by co-transfection of heavy chain and light chain plasmids. All versions of hu24F4 were assembled and secreted, with titers exceeding that of ch24F4 (determined by quantitation of mAb in conditioned medium by Octet binding to anti-human Fc tips). Western blots of non-reducing SDS-PAGE analysis of chimeric and humanized 24F4 mAbs showed no evidence of gross aggregation or obvious clipping relative to ch24F4.
Conditioned medium was assayed by direct binding FACS on stably transfected DG44 CHO cells co-expressing full-length BDCA2 and FcεRIγ cDNAs (human or cynomolgus monkey), (relevant expression vectors are human BDCA2/FcεRIγ: pEAG2456, cyno BDCA2/FcεRIγ: pEAG2668). In direct binding to surface human or cynomolgus monkey BDCA2, a complete loss in binding was observed for the H0, H1 and H2 series of hu24F4, a significant loss of binding affinity was observed for the H3 series of hu24F4, good retention of affinity for both the H4 and H5 series of hu24F4 and a moderate loss of binding for the H6 series of hu24F4 variants (FIGS. 2 and 3). Based upon titer and apparent EC50 values in direct binding FACS analysis, H4/L1 and H5/L1 were determined as the “best” variants of hu24F4.
Conditioned medium containing ch24F4 and all hu24F4 variant mAbs was assayed by Octet for binding to human BDCA2 ectodomain. The monomeric huBDCA2 ectodomain was prepared by proteolytic cleavage from the purified muIgG2a Fc-huBDCA2 fusion protein (relevant plasmid: pEAG2423). Antibodies from conditioned medium from transiently transfected cells were bound to anti-human Fc tips, and then monomeric huBDCA2 was flowed over the Octet tips, to examine binding and dissociation. The H4 and H5 series of hu24F4 variants showed the best affinities for huBDCA2.
















Sample name
KD (M)
kon (1/Ms)
kdis (1/s)









H6/L0
5.00E−09
2.73E+05
1.37E−03



H0/L1
9.50E−11
1.00E+05
9.50E−06



H1/L1
5.03E−11
1.00E+05
5.03E−06



H2/L1
3.35E−11
1.00E+05
3.35E−06



H3/L1
1.30E−08
4.52E+05
5.86E−03



H4/L1
7.44E−10
5.49E+05
4.08E−04



ch24F4
2.17E−09
1.61E+06
3.49E−03



5C8 control
2.51E−14
1.00E+05
2.51E−09










Example 6. Enhancing hu24F4 Affinity
To explore the possibility of enhancing hu24F4 affinity by substitution at the position of the 24F4 version L1 CDR L3 unpaired cysteine (C95S in hu24F4 light chain expression vector pYL740), a number of version L1 variants were constructed by site-directed mutagenesis. Backmutation to the unpaired cysteine, i.e., S95C, was constructed by site-directed mutagenesis producing plasmid pYL749. Variants S95T, S95A, and S95V were constructed by site-directed mutagenesis producing plasmids pYL750, pYL751, and pYL752, respectively. The full-length light chain cDNA sequences in the resultant plasmids were confirmed by DNA sequencing. C95 variant hu24F4 mAbs were expressed transiently in 293E cells by co-transfection of hu24F4 H4 heavy chain pYL746 or hu24F4 H5 heavy chain pYL747 with hu24F4 L1 variant light chains C95S pYL740, S95C pYL749, S95T pYL750, S95A pYL751 or S95V pYL752 plasmids. Conditioned medium was harvested at 2 days post-transfection. Titers (assayed by Octet on anti-human Fc tips) of all variants were similar, and Western blots of nonreducing SDS-PAGE indicated no gross aggregation or obvious clipping. Conditioned medium containing the C95 variant mAbs was assayed by Octet for binding to human BDCA2 ectodomain. Antibodies from conditioned medium from transiently transfected cells were bound to anti-human Fc tips, then monomeric huBDCA2 was flowed over the Octet tips, to examine binding and dissociation. C95A variants had the slowest off-rates.













Sample name
KD (M)
kon (1/Ms)
kdis (1/s)







24F4-H4/L1 (YL740/YL746)
5.48E−10
7.27E+05
3.98E−04


H4-L1-S95C (YL749/YL746)
2.89E−10
9.67E+05
2.79E−04


H4-L1-C95T (YL750/YL746)
3.92E−10
9.44E+05
3.70E−04


H4-L1-C95A (YL751/YL746)
2.61E−10
8.84E+05
2.30E−04


H4-L1-C95V (YL752/YL746)
3.23E−10
9.33E+05
3.01E−04









Based upon these results, stable CHO cell lines were produced for the hu24F4 H4/L1 C95T and C95A variants and H5/L1 C95T and C95A variants, which had the slowest apparent off-rates. Octet binding studies were repeated for purified hu24F4 mAbs. The hu24F4 H4/L1 C95A variant was selected as the lead candidate. Sequences of plasmids pYL746 (hu 24F4 H4 heavy chain) and pYL751 ((hu 24F4 L1 light chain) were used for recoding and construction of expression vectors for CHO production cell line generation.
The deduced mature hu24F4 L1 C95A light chain amino acid sequence encoded by pYL751 is shown below (CDR L1, CDR L2, and CDR L3 are underlined):








(SEQ ID NO: 3)










  1 
DIQLTQSPSS LSASVGDRVT ITCKASQSVD YDGDSYMNWY QQKPGKAPKL



 


 51
LIYAASTLES GVPSRFSGSG SGTDFTLTIS SLQPEDFATY YCQQANEDPR


 


101
TFGQGTKVEI KRTVAAPSVF IFPPSDEQLK SGTASVVCLL NNFYPREAKV


 


151
QWKVDNALQS GNSQESVTEQ DSKDSTYSLS STLTLSKADY EKHKVYACEV


 


201
THQGLSSPVT KSFNRGEC






The deduced mature hu24F4 H4-hulgG1 heavy chain amino acid sequence encoded by pYL746 is shown below (CDR H1; CDR H2, and CDR H3 are underlined):








(SEQ ID NO: 4)










1
DVQLVESGGG LVKPGGSLRL SCAASGFTFS TYTMSWVRQA PGKGLEWVAT



 


51
ISPGDSFGYY YPDSVQGRFT ISRDNAKNSL YLQMNSLRAE DTAVYYCTRD


 


101
IYYNYGAWFA YWGQGTLVTV SSASTKGPSV FPLAPSSKST SGGTAALGCL


 


151
VKDYFPEPVT VSWNSGALTS GVHTFPAVLQ SSGLYSLSSV VTVPSSSLGT


 


201
QTYICNVNHK PSNTKVDKKV EPKSCDKTHT CPPCPAPELL GGPSVFLFPP


 


251
KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ


 


301
YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE


 


351
PQVYTLPPSR DELTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP


 


401
PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP


 


451
G






An antibody consisting of the mature heavy chain (SEQ ID NO: 4) and the mature light chain (SEQ ID NO: 3) listed above is termed BIIB059.
Example 7. Recoding the Heavy and Light Chain Genes
To potentially improve expression, the nucleotide sequence of the light and heavy chain genes were recoded without changing the amino acid sequence. The modified DNA sequence for the anti-BDCA2 light chain gene is shown below. Amino acids 1-240 contain the light chain sequence Amino acids 1-22 (nucleotides in lower case) contain the native light chain signal peptide. The mature N-terminus begins with amino acid 23 (D).








1 
atg gac atg agg gtc ccc get cag ctc ctg ggg


1>
 M   D   M   R   V   P   A   Q   L   L   G


 



ctc ctt ctg ctc tgg ctc cct gga gca cga tgt



 L   L   L   L   W   L   P   G   A   R   C


 


67 
GAC ATT CAG CTG ACC CAA TCT CCA TCC TCT TTG


23>
 D   I   Q   L   T   Q   S   P   S   S   L


 



TCC GCC TCT GTG GGG GAC AGG GTC ACC ATC ACC



 S   A   S   V   G   D   R   V   T   I   T


 


133 
TGC AAG GCC AGC CAA AGT GTT GAT TAT GAT GGT


45>
C   K   A   S   Q   S   V   D   Y   D   G 


 



GAT AGT TAT ATG AAC TGG TAT CAA CAG AAA CCA



D   S   Y   M   N   W   Y   Q   Q   K   P


 


199 
GGG AAG GCT CCC AAA CTC CTC ATC TAC GCT GCA


67>
G   K   A   P   K   L   L   I   Y   A   A


 



TCC ACT CTC GAG TCT GGG GTC CCA TCC AGG TTT



S   T   L   E   S   G   V   P   S   R   F


 


265 
AGT GGC AGT GGG TCT GGG ACA GAC TTC ACC CTC


89>
S   G   S   G   S   G   T   D   F   T   L


 



ACA ATC AGC TCA CTC CAG CCA GAG GAT TTC GCA



T   I   S   S   L   Q   P   E   D   F   A


 


331 
ACC TAT TAC TGT CAA CAA GCC AAC GAA GAT CCT


111>
 T   Y   Y   C   Q   Q   A   N   E   D   P


 



CGG ACC TTC GGT CAG GGC ACC AAA GTG GAA ATC



 R   T   F   G   Q   G   T   K   V   E   I


 


397 
AAG CGG ACC GTG GCT GCA CCA TCT GTC TTC ATC


133>
 K   R   T   V   A   A   P   S   V   F   I


 



TTC CCT CCA TCT GAT GAG CAG TTG AAA TCT GGA



 F   P   P   S   D   E   Q   L   K   S   G


 


463 
ACT GCC TCT GTT GTG TGC CTG CTG AAT AAC TTC 


155>
 T   A   S   V   V   C   L   L   N   N   F   


 



TAT CCC AGA GAG GCC AAA GTG CAG TGG AAG GTG



 Y   P   R   E   A   K   V   Q   W   K   V


 


529 
GAT AAC GCC CTC CAA TCT GGC AAC TCC CAG GAG


177>
 D   N   A   L   Q   S   G   N   S   Q   E


 



AGT GTC ACA GAG CAG GAC AGC AAG GAC AGC ACC



 S   V   T   E   Q   D   S   K   D   S   T


 


595 
TAC AGC CTC AGC AGC ACC CTG ACC CTG AGC AAA 


199>
 Y   S   L   S   S   T   L   T   L   S   K


 



GCA GAC TAC GAG AAA CAC AAA GTC TAC GCC TGC



 A   D   Y   E   K   H   K   V   Y   A   C


 


661 
GAA GTC ACC CAT CAG GGC CTG AGC TCT CCC GTC 


221>
 E   V   T   H   Q   G   L   S   S   P   V 


 



ACA AAG AGC TTC AAC AGG GGA GAG TGT TGA



 T   K   S   F   N   R   G   E   C   *


 



(SEQ ID NO: 60)



(SEQ ID NO: 61)






The modified DNA sequence for the anti-BDCA2 heavy chain gene is shown below. Amino acids 1-470 contain the heavy chain sequence. Amino acids 1-19 (nucleotides in lower case) contain the native heavy chain signal peptide. The mature N-terminus begins with amino acid 20 (D).








1 
atg ggt tgg agc ctc atc ttg ctc ttc ctt gtc 


1>
 M   G   W   S   L   I   L   L   F   L   V


 



gct gtt gct acc cgg gtc ctg tcc GAC GTC CAG



 A   V   A   T   R   V   L   S   D   V   Q


 


67 
CTG GTG GAG TCT GGG GGA GGC CTG GTG AAG CCT 


23>
 L   V   E   S   G   G   G   L   V   K   P


 



GGA GGG TCC CTG AGA CTC TCC TGC GCA GCC TCT



 G   G   S   L   R   L   S   C   A   A   S


 


133 
GGA TTC ACT TTC AGT ACC TAT ACC ATG TCT TGG 


45>
 G   F   T   F   S   T   Y   T   M   S   W


 



GTT CGC CAA GCA CCT GGC AAG GGA CTG GAG TGG



 V   R   Q   A   P   G   K   G   L   E   W


 


199 
GTC GCA ACC ATT AGT CCA GGA GAC AGT TTC GGC 


67>
 V   A   T   I   S   P   G   D   S   F   G


 



TAC TAC TAT CCA GAC AGT GTC CAG GGC CGA TTC



 Y   Y   Y   P   D   S   V   Q   G   R   F


 


265 
ACC ATC TCC AGA GAC AAT GCC AAG AAC AGT CTG 


89>
 T   I   S   R   D   N   A   K   N   S   L 


 



TAC CTG CAA ATG AAC AGT CTG AGG GCA GAG GAC



 Y   L   Q   M   N   S   L   R   A   E   D


 


331 
ACA GCC GTG TAT TAC TGT ACC CGA GAT ATT TAC 


111>
 T   A   V   Y   Y   C   T   R   D   I   Y


 



TAT AAT TAC GGA GCC TGG TTT GCT TAC TGG GGC



 Y   N   Y   G   A   W   F   A   Y   W   G


 


397 
CAA GGG ACT CTG GTC ACT GTC TCT AGC GCT TCC 


133>
 Q   G   T   L   V   T   V   S   S   A   S


 



ACC AAG GGC CCA TCC GTC TTC CCC CTG GCA CCC



 T   K   G   P   S   V   F   P   L   A   P


 


463 
TCC TCC AAG AGC ACC TCT GGG GGC ACA GCT GCC 


155>
 S   S   K   S   T   S   G   G   T   A   A 


 



CTG GGC TGC CTG GTC AAG GAC TAC TTC CCC GAA



 L   G   C   L   V   K   D   Y   F   P   E


 


529 
CCC GTG ACC GTG TCC TGG AAC TCA GGC GCC CTG 


177>
 P   V   T   V   S   W   N   S   G   A   L


 



ACC AGC GGC GTG CAC ACC TTC CCC GCT GTC CTG



 T   S   G   V   H   T   F   P   A   V   L


 


595 
CAA TCC TCA GGA CTC TAC TCC CTC TCC AGC GTG 


199>
 Q   S   S   G   L   Y   S   L   S   S   V


 



GTG ACC GTG CCC TCC AGC AGC TTG GGC ACC CAG



 V   T   V   P   S   S   S   L   G   T   Q


 


661 
ACC TAC ATC TGC AAC GTG AAT CAC AAG CCC AGC 


221>
 T   Y   I   C   N   V   N   H   K   P   S


 



AAC ACC AAG GTG GAC AAG AAA GTT GAG CCC AAA



 N   T   K   V   D   K   K   V   E   P   K


 


727 
TCT TGT GAC AAG ACT CAC ACA TGC CCA CCT TGC 


243>
 S   C   D   K   T   H   T   C   P   P   C


 



CCA GCA CCT GAA CTC CTG GGG GGA CCT TCA GTC



 P   A   P   E   L   L   G   G   P   S   V


 


793 
TTC CTC TTC CCC CCA AAA CCC AAG GAC ACC CTC 


265>
 F   L   F   P   P   K   P   K   D   T   L


 



ATG ATC TCC CGG ACC CCT GAG GTC ACA TGC GTG



 M   I   S   R   T   P   E   V   T   C   V


 


859 
GTG GTG GAC GTG AGC CAC GAA GAC CCT GAG GTC 


287>
 V   V   D   V   S   H   E   D   P   E   V


 



AAG TTC AAC TGG TAT GTT GAC GGC GTG GAG GTC



 K   F   N   W   Y   V   D   G   V   E   V


 


925 
CAT AAT GCC AAG ACA AAG CCT CGG GAG GAG CAG 


309>
 H   N   A   K   T   K   P   R   E   E   Q


 



TAC AAC AGC ACC TAC CGG GTG GTC AGC GTC CTC



 Y   N   S   T   Y   R   V   V   S   V   L


 


991 
ACC GTC CTG CAC CAA GAC TGG CTG AAT GGC AAG 


331>
 T   V   L   H   Q   D   W   L   N   G   K


 



GAG TAC AAG TGC AAG GTC TCC AAC AAA GCC CTC



 E   Y   K   C   K   V   S   N   K   A   L


 


1057 
CCA GCC CCC ATC GAG AAA ACC ATC TCC AAA GCC


353>
 P   A   P   I   E   K   T   I   S   K   A


 



 AAA GGG CAG CCC CGA GAA CCA CAG GTG TAC ACC



 K   G   Q   P   R   E   P   Q   V   Y   T


 


1123 
CTG CCC CCA TCC CGG GAT GAG CTG ACC AAG AAC 


375>
 L   P   P   S   R   D   E   L   T   K   N


 



CAA GTC AGC CTG ACC TGC CTG GTC AAA GGC TTC



 Q   V   S   L   T   C   L   V   K   G   F


 


1189 
TAT CCC AGC GAC ATC GCC GTG GAG TGG GAG AGC 


397>
 Y   P   S   D   I   A   V   E   W   E   S


 



AAT GGG CAG CCT GAG AAC AAC TAC AAG ACC ACA



 N   G   Q   P   E   N   N   Y   K   T   T


 


1255 
CCT CCC GTG TTG GAC TCC GAC GGC TCC TTC TTC 


419>
 P   P   V   L   D   S   D   G   S   F   F   


 



CTC TAC TCC AAG CTC ACC GTG GAC AAG AGC AGG



 L   Y   S   K   L   T   V   D   K   S   R


 


1321 
TGG CAG CAG GGG AAC GTC TTC TCA TGC TCC GTG 


441>
 W   Q   Q   G   N   V   F   S   C   S   V


 



ATG CAT GAG GCT CTG CAC AAC CAC TAC ACC CAG



 M   H   E   A   L   H   N   H   Y   T   Q


 


1387 
AAG AGC CTC TCC CTG TCT CCC GGT TGA



(SEQ ID NO: 62)


463>
 K   S   L   S   L   S   P   G   *



(SEQ ID NO: 63)






Example 8. Expression Cassettes and Vectors
The heavy chain gene and the light chain genes were excised and ligated into separate expression vectors. Each gene is under transcriptional control of the human cytomegalovirus immediate-early promoter and the human growth hormone gene polyadenylation sequence.
The plasmid expressing the light chain, pJP009, also contains an expression cassette for the neomycin phosphotransferase gene (neo) containing the murine phosphoglycerate kinase (muPGK) early promoter and the muPGK polyadenylation sequence (FIG. 4). The plasmid expressing the heavy chain, pJP010, also contains an expression cassette for the dhfr gene which was used as a selectable and methotrexate-amplifiable marker. The key features of plasmids pJP009 and pJP010 are summarized below.


















Mature




Plasmid


Polypeptide
Poly-
Selectable


Name
Promoters
Signal Peptides
Chain
adenylation
Markers







pJP009
hCMV IE
Native human
Light chain
hGH
Neomycin



muPGK
kappa subgroup I
218 aa
muPGK
phosphotransferase:







(G418)







beta-lactamase:







(ampicillin)


pJP010
hCMV IE
Synthetic signal
Heavy chain
hGH
DHFR: (alpha-



SV40E
peptide sequence
451 aa
SV40E
nucleosides)







beta-lactamase:







(ampicillin)





Abbreviations:


human cytomegalovirus immediate early (hCMV IE),


early simian virus 40 (SV40E),


murine phosphoglycerate kinase (muPGK),


human growth hormone (hGH),


neomycin phosphotransferase gene (G418 resistance),


dihydrofolate reductase gene (dhfr),


bacterial gene for resistance to ampicillin (beta-lactamase).






The complete nucleotide sequence of plasmid pJP009 is shown below. The three open reading frames are the 24F4 light chain, neomycin phosphotransferase, and beta-lactamase.









1 
TTGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTA



 


93 
CGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACT


 


185
TTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGA



 


277
CGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCA


 


369
TCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATT


 


461 
GACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAG



 


553
GCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGATCGCCTGGAGACGCCATCCACGCTGTTTTGACCTCCATA



 


645
GAAGACACCGGGACCGATCCAGCCTCCGCGGCCGGGAACGGTGCATTGGAACGCGGATTCCCCGTGCCAAGAGTGACGTAAGTACCGCCTAT


 


737
AGAGTCTATAGGCCCACCCCCTTGGCTTCTTATGCATGCTATACTGTTTTTGGCTTGGGGTCTATACACCCCCGCTTCCTCATGTTATAGGT


 


829
GATGGTATAGCTTAGCCTATAGGTGTGGGTTATTGACCATTATTGACCACTCCCCTATTGGTGACGATACTTTCCATTACTAATCCATAACA


 


921 
TGGCTCTTTGCCACAACTCTCTTTATTGGCTATATGCCAATACACTGTCCTTCAGAGACTGACACGGACTCTGTATTTTTACAGGATGGGGT



 


1013
CTCATTTATTATTTACAAATTCACATATACAACACCACCGTCCCCAGTGCCCGCAGTTTTTATTAAACATAACGTGGGATCTCCACGCGAAT



 


1105
CTCGGGTACGTGTTCCGGAACGGTGGAGGGCAGTGTAGTCTGAGCAGTACTCGTTGCTGCCGCGCGCGCCACCAGACATAATAGCTGACAGA


 


1197
CTAACAGACTGTTCCTTTCCATGGGTCTTTTCTGCAGTCACCGTCCTTGACACGGGATCCGCCACC ATG GAC ATG AGG GTC CCC



                                                                  > M   D   M   R   V   P


 


1281 
 GCT CAG CTC CTG GGG CTC CTT CTG CTC TGG CTC CCT GGA GCA CGA TGT GAC ATT CAG CTG ACC CAA TCT




> A   Q   L   L   G   L   L   L   L   W   L   P   G   A   R   C   D   I   Q   L   T   Q   S



 


1350
 CCA TCC TCT TTG TCC GCC TCT GTG GGG GAC AGG GTC ACC ATC ACC TGC AAG GCC AGC CAA AGT GTT GAT



> P   S   S   L   S   A   S   V   G   D   R   V   T   I   T   C   K   A   S   Q   S   V   D


 


1419
 TAT GAT GGT GAT AGT TAT ATG AAC TGG TAT CAA CAG AAA CCA GGG AAG GCT CCC AAA CTC CTC ATC TAC



> Y   D   G   D   S   Y   M   N   W   Y   Q   Q   K   P   G   K   A   P   K   L   L   I   Y


 


1488
 GCT GCA TCC ACT CTC GAG TCT GGG GTC CCA TCC AGG TTT AGT GGC AGT GGG TCT GGG ACA GAC TTC ACC



> A   A   S   T   L   E   S   G   V   P   S   R   F   S   G   S   G   S   G   T   D   F   T


 


1557 
 CTC ACA ATC AGC TCA CTC CAG CCA GAG GAT TTC GCA ACC TAT TAC TGT CAA CAA GCC AAC GAA GAT CCT




> L   T   I   S   S   L   Q   P   E   D   F   A   T   Y   Y   C   Q   Q   A   N   E   D   P



 


1626
 CGG ACC TTC GGT CAG GGC ACC AAA GTG GAA ATC AAG CGG ACC GTG GCT GCA CCA TCT GTC TTC ATC TTC



> R   T   F   G   Q   G   T   K   V   E   I   K   R   T   V   A   A   P   S   V   F   I   F


 


1695
 CCT CCA TCT GAT GAG CAG TTG AAA TCT GGA ACT GCC TCT GTT GTG TGC CTG CTG AAT AAC TTC TAT CCC



> P   P   S   D   E   Q   L   K   S   G   T   A   S   V   V   C   L   L   N   N   F   Y   P


 


1764
 AGA GAG GCC AAA GTG CAG TGG AAG GTG GAT AAC GCC CTC CAA TCT GGC AAC TCC CAG GAG AGT GTC ACA



> R   E   A   K   V   Q   W   K   V   D   N   A   L   Q   S   G   N   S   Q   E   S   V   T


 


1833
 GAG CAG GAC AGC AAG GAC AGC ACC TAC AGC CTC AGC AGC ACC CTG ACC CTG AGC AAA GCA GAC TAC GAG



> E   Q   D   S   K   D   S   T   Y   S   L   S   S   T   L   T   L   S   K   A   D   Y   E


 


1902
 AAA CAC AAA GTC TAC GCC TGC GAA GTC ACC CAT CAG GGC CTG AGC TCT CCC GTC ACA AAG AGC TTC AAC



> K   H   K   V   Y   A   C   E   V   T   H   Q   G   L   S   S   P   V   T   K   S   F   N


 


1971
 AGG GGA GAG TGT TGA GGATCCCTGCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGTCGTGGAAGGTGCTACTCCA



> R   G   E   C   • (SEQ ID NO: 64)


 


2058
GTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTTTGACTAGGTGTCCTTGTATAATATTATGGGGTGGAGGCGGGTGGT


 


2150
ATGGAGCAAGGGGCAGGTTGGGAAGACAACCTGTAGGGCCTTCAGGGTCTATTGGGAACCAGGCTGGAGTGCAGTGGCACGATCTTGGCTCG


 


2242
CTGCAATCTCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGTCTCCCGAATAGTTGGGATTCCAGGCATGCACGACCAGGCTCAGCTAA


 


2334
TTTTTGTATTTTTGGTAGAGACGGGGTTTCACCATATTGGCCAGTCTGGTCTCCATCTCCTGACCTCAGGTAATCCGCCCGCCTCGGCCTCC


 


2426
CAAATTGCTGGGATTACAGGTATGAGCCACTGGGCCCTTCCCTGTCCTGTGATTTTAAAATAATTATACCAGCAGAAGGACGTCCAGACACA


 


2518
GCATGGGCTACCTGGCCATGCCCAGCCAGTTGGACATTTGAGTTGTTTGCTTGGCACTGTCCTCTCATGAATTCCTGCAGGATTCGAGGGCC


 


2610
CCTGCAGGTCAATTCTACCGGGTAGGGGAGGCGCTTTTCCCAAGGCAGTCTGGAGCATGCGCTTTAGCAGCCCCGCTGGGCACTTGGCGCTA


 


2702
CACAAGTGGCCTCTGGCCTCGCACACATTCCACATCCACCGGTAGGCGCCAACCGGCTCCGTTCTTTGGTGGCCCCTTCGCGCCACCTTCTA


 


2794
CTCCTCCCCTAGTCAGGAAGTTCCCCCCCGCCCCGCAGCTCGCGTCGTGCAGGACGTGACAAATGGAAGTAGCACGTCTCACTAGTCTCGTG


 


2886
CAGATGGACAGCACCGCTGAGCAATGGAAGCGGGTAGGCCTTTGGGGCAGCGGCCAATAGCAGCTTTGCTCCTTCGCTTTCTGGGCTCAGAG


 


2978
GCTGGGAAGGGGTGGGTCCGGGGGCGGGCTCAGGGGCGGGCTCAGGGGCGGGGCGGGCGCCCGAAGGTCCTCCGGAGGCCCGGCATTCTGCA


 


3070
CGCTTCAAAAGCGCACGTCTGCCGCGCTGTTCTCCTCTTCCTCATCTCCGGGCCTTTCGACCTGCAGCCAATATGGGATCGGCCATTGAACA



                                                                       > M  G  S  A  I  E  Q


 


3162
 AGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCG



>  D  G  L  H  A  G  S  P  A  A  W  V  E  R  L  F  G  Y  D  W  A  Q  Q  T  I  G  C  S  D  A


 


3254
 CCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCG



>A  V  F  R  L  S  A  Q  G  R  P  V  L  F  V  K  T  D  L  S  G  A  L  N  E  L  Q  D  E  A  A


 


3346 
 CGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGA




> R  L  S  W  L  A  T  T  G  V  P  C  A  A  V  L  D  V  V  T  E  A  G  R  D  W  L  L  L  G  E



 


3438
 AGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTG



>  V  P  G  Q  D  L  L  S  S  H  L  A  P  A  E  K  V  S  I  M  A  D  A  M  R  R  L  H  T  L


 


3530
 ATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGAT



>D  P  A  T  C  P  F  D  H  Q  A  K  H  R  I  E  R  A  R  T  R  M  E  A  G  L  V  D  Q  D  D


 


3622
 CTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGATGATCTCGTCGTGACCCA



> L  D  E  E  H  Q  G  L  A  P  A  E  L  F  A  R  L  K  A  R  M  P  D  G  D  D  L  V  V  T  H


 


3714
 TGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATC



>  G  D  A  C  L  P  N  I  M  V  E  N  G  R  F  S  G  F  I  D  C  G  R  L  G  V  A  D  R  Y


 


3806
 AGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCC



>Q  D  I  A  L  A  T  R  D  I  A  E  E  L  G  G  E  W  A  D  R  F  L  V  L  Y  G  I  A  A  P


 


3898
 GATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGGGGATCGATCCGCTGTAAGTCTGCAGAAATTGATGATCTATTA



> D  S  Q  R  I  A  F  Y  R  L  L  D  E  F  F  • (SEQ ID NO: 65)


 


3990
AACAATAAAGATGTCCACTAAAATGGAAGTTTTTCCTGTCATACTTTGTTAAGAAGGGTGAGAACAGAGTACCTACATTTTGAATGGAAGGA


 


4082
TTGGAGCTACGGGGGTGGGGGTGGGGTGGGATTAGATAAATGCCTGCTCTTTACTGAAGGCTCTTTACTATTGCTTTATGATAATGTTTCAT


 


4174
AGTTGGATATCATAATTTAAACAAGCAAAACCAAATTAAGGGCCAGCTCATTCCTCCCACTCATGATCTATAGATCTATAGATCTCTCGTGG


 


4266
GATCATTGTTTTTCTCTTGATTCCCACTTTGTGGTTCTAAGTACTGTGGTTTCCAAATGTGTCAGTTTCATAGCCTGAAGAACGAGATCAGC


 


4358
AGCCTCTGTTCCACATACACTTCATTCTCAGTATTGTTTTGCCAAGTTCTAATTCCATCAGAAGCTGACTCTAGATCTGGATCGATGAATTC


 


4450
GGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCA


 


4542
TAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGAC


 


4634
CGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGG


 


4726
TTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA


 


4818
CATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCG



                                                                       > M  S  I  Q  H  F  R


 


4910
 TGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGT



>  V  A  L  I  P  F  F  A  A  F  C  L  P  V  F  A  H  P  E  T  L  V  K  V  K  D  A  E  D  Q


 


5002
 TGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGC



>L  G  A  R  V  G  Y  I  E  L  D  L  N  S  G  K  I  L  E  S  F  R  P  E  E  R  F  P  M  M  S


 


5094
 ACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGA



> T  F  K  V  L  L  C  G  A  V  L  S  R  I  D  A  G  Q  E  Q  L  G  R  R  I  H  Y  S  Q  N  D


 


5186
 CTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATA



>  L  V  E  Y  S  P  V  T  E  K  H  L  T  D  G  M  T  V  R  E  L  C  S  A  A  I  T  M  S  D


 


5278
 ACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT



>N  T  A  A  N  L  L  L  T  T  I  G  G  P  K  E  L  T  A  F  L  H  N  M  G  D  H  V  T  R  L


 


5370
 GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACT



> D  R  W  E  P  E  L  N  E  A  I  P  N  D  E  R  D  T  T  M  P  V  A  M  A  T  T  L  R  K  L


 


5462
 ATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGG



>  L  T  G  E  L  L  T  L  A  S  R  Q  Q  L  I  D  W  M  E  A  D  K  V  A  G  P  L  L  R  S


 


5554
 CCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAG



>A  L  P  A  G  W  F  I  A  D  K  S  G  A  G  E  R  G  S  R  G  I  I  A  A  L  G  P  D  G  K


 


5646
 CCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGAT



> P  S  R  I  V  V  I  Y  T  T  G  S  Q  A  T  M  D  E  R  N  R  Q  I  A  E  I  G  A  S  L  I


 


5738
 TAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGA



>  K  H  W  • (SEQ ID NO: 66)


 


5830
TCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCT


 


5922
TGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACC


 


6014
AACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGA


 


6106
ACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGAC


 


6198
TCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGA


 


6290
ACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAA


 


6382
CAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTT


 


6474
TTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCA


 


6566
CATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCG


 


6658
AGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGG


 


6750
CACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTT


 


6842
TATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGC 



(SEQ ID NO: 67)






The complete nucleotide sequence of plasmid pJP010 (FIG. 5) is shown below. The three open reading frames are the 24F4 heavy chain, murine dihydrofolate reductase, and beta-lactamase.









1
TTGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTA



 


93 
CGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACT



 


185
TTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGA



 


277
CGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCA


 


369
TCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATT


 


461
GACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAG


 


553
GCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGATCGCCTGGAGACGCCATCCACGCTGTTTTGACCTCCATA


 


645
GAAGACACCGGGACCGATCCAGCCTCCGCGGCCGGGAACGGTGCATTGGAACGCGGATTCCCCGTGCCAAGAGTGACGTAAGTACCGCCTAT


 


737
AGAGTCTATAGGCCCACCCCCTTGGCTTCTTATGCATGCTATACTGTTTTTGGCTTGGGGTCTATACACCCCCGCTTCCTCATGTTATAGGT


 


829
GATGGTATAGCTTAGCCTATAGGTGTGGGTTATTGACCATTATTGACCACTCCCCTATTGGTGACGATACTTTCCATTACTAATCCATAACA


 


921 
TGGCTCTTTGCCACAACTCTCTTTATTGGCTATATGCCAATACACTGTCCTTCAGAGACTGACACGGACTCTGTATTTTTACAGGATGGGGT



 


1013
CTCATTTATTATTTACAAATTCACATATACAACACCACCGTCCCCAGTGCCCGCAGTTTTTATTAAACATAACGTGGGATCTCCACGCGAAT



 


1105
CTCGGGTACGTGTTCCGGAACGGTGGAGGGCAGTGTAGTCTGAGCAGTACTCGTTGCTGCCGCGCGCGCCACCAGACATAATAGCTGACAGA


 


1197
CTAACAGACTGTTCCTTTCCATGGGTCTTTTCTGCAGTCACCGTCCTTGACACGGGATCCGCCACC ATG GGT TGG AGC CTC ATC



                                                                  > M   G   W   S   L   I


 


1281
 TTG CTC TTC CTT GTC GCT GTT GCT ACC CGG GTC CTG TCC GAC GTC CAG CTG GTG GAG TCT GGG GGA GGC



> L   L   F   L   V   A   V   A   T   R   V   L   S   D   V   Q   L   V   E   S   G   G   G


 


1350 
 CTG GTG AAG CCT GGA GGG TCC CTG AGA CTC TCC TGC GCA GCC TCT GGA TTC ACT TTC AGT ACC TAT ACC




> L   V   K   P   G   G   S   L   R   L   S   C   A   A   S   G   F   T   F   S   T   Y   T   



 


1419
 ATG TCT TGG GTT CGC CAA GCA CCT GGC AAG GGA CTG GAG TGG GTC GCA ACC ATT AGT CCA GGA GAC AGT



>  M   S   W   V   R   Q   A   P   G   K   G   L   E   W   V   A   T   I   S   P   G   D   S


 


1488
 TTC GGC TAC TAC TAT CCA GAC AGT GTC CAG GGC CGA TTC ACC ATC TCC AGA GAC AAT GCC AAG AAC AGT



>  F   G   Y   Y   Y   P   D   S   V   Q   G   R   F   T   I   S   R   D   N   A   K   N   S


 


1557
 CTG TAC CTG CAA ATG AAC AGT CTG AGG GCA GAG GAC ACA GCC GTG TAT TAC TGT ACC CGA GAT ATT TAC



>  L   Y   L   Q   M   N   S   L   R   A   E   D   T   A   V   Y   Y   C   T   R   D   I   Y


 


1626
 TAT AAT TAC GGA GCC TGG TTT GCT TAC TGG GGC CAA GGG ACT CTG GTC ACT GTC TCT AGC GCT TCC ACC



>  Y   N   Y   G   A   W   F   A   Y   W   G   Q   G   T   L   V   T   V   S   S   A   S   T


 


1695
 AAG GGC CCA TCC GTC TTC CCC CTG GCA CCC TCC TCC AAG AGC ACC TCT GGG GGC ACA GCT GCC CTG GGC



>  K   G   P   S   V   F   P   L   A   P   S   S   K   S   T   S   G   G   T   A   A   L   G


 


1764
 TGC CTG GTC AAG GAC TAC TTC CCC GAA CCC GTG ACC GTG TCC TGG AAC TCA GGC GCC CTG ACC AGC GGC



> C   L   V   K   D   Y   F   P   E   P   V   T   V   S   W   N   S   G   A   L   T   S   G


 


1833
 GTG CAC ACC TTC CCC GCT GTC CTG CAA TCC TCA GGA CTC TAC TCC CTC TCC AGC GTG GTG ACC GTG CCC



> V   H   T   F   P   A   V   L   Q   S   S   G   L   Y   S   L   S   S   V   V   T   V   P


 


1902
 TCC AGC AGC TTG GGC ACC CAG ACC TAC ATC TGC AAC GTG AAT CAC AAG CCC AGC AAC ACC AAG GTG GAC



> S   S   S   L   G   T   Q   T   Y   I   C   N   V   N   H   K   P   S   N   T   K   V   D


 


1971
 AAG AAA GTT GAG CCC AAA TCT TGT GAC AAG ACT CAC ACA TGC CCA CCT TGC CCA GCA CCT GAA CTC CTG



> K   K   V   E   P   K   S   C   D   K   T   H   T   C   P   P   C   P   A   P   E   L   L


 


2040
 GGG GGA CCT TCA GTC TTC CTC TTC CCC CCA AAA CCC AAG GAC ACC CTC ATG ATC TCC CGG ACC CCT GAG



> G   G   P   S   V   F   L   F   P   P   K   P   K   D   T   L   M   I   S   R   T   P   E


 


2109 
 GTC ACA TGC GTG GTG GTG GAC GTG AGC CAC GAA GAC CCT GAG GTC AAG TTC AAC TGG TAT GTT GAC GGC




> V   T   C   V   V   V   D   V   S   H   E   D   P   E   V   K   F   N   W   Y   V   D   G



 


2178
 GTG GAG GTC CAT AAT GCC AAG ACA AAG CCT CGG GAG GAG CAG TAC AAC AGC ACC TAC CGG GTG GTC AGC



> V   E   V   H   N   A   K   T   K   P   R   E   E   Q   Y   N   S   T   Y   R   V   V   S


 


2247
 GTC CTC ACC GTC CTG CAC CAA GAC TGG CTG AAT GGC AAG GAG TAC AAG TGC AAG GTC TCC AAC AAA GCC



> V   L   T   V   L   H   Q   D   W   L   N   G   K   E   Y   K   C   K   V   S   N   K   A


 


2316
 CTC CCA GCC CCC ATC GAG AAA ACC ATC TCC AAA GCC AAA GGG CAG CCC CGA GAA CCA CAG GTG TAC ACC



> L   P   A   P   I   E   K   T   I   S   K   A   K   G   Q   P   R   E   P   Q   V   Y   T


 


2385
 CTG CCC CCA TCC CGG GAT GAG CTG ACC AAG AAC CAA GTC AGC CTG ACC TGC CTG GTC AAA GGC TTC TAT



> L   P   P   S   R   D   E   L   T   K   N   Q   V   S   L   T   C   L   V   K   G   F   Y


 


2454 
 CCC AGC GAC ATC GCC GTG GAG TGG GAG AGC AAT GGG CAG CCT GAG AAC AAC TAC AAG ACC ACA CCT CCC




> P   S   D   I   A   V   E   W   E   S   N   G   Q   P   E   N   N   Y   K   T   T   P   P



 


2523
 GTG TTG GAC TCC GAC GGC TCC TTC TTC CTC TAC TCC AAG CTC ACC GTG GAC AAG AGC AGG TGG CAG CAG



> V   L   D   S   D   G   S   F   F   L   Y   S   K   L   T   V   D   K   S   R   W   Q   Q


 


2592
 GGG AAC GTC TTC TCA TGC TCC GTG ATG CAT GAG GCT CTG CAC AAC CAC TAC ACC CAG AAG AGC CTC TCC



> G   N   V   F   S   C   S   V   M   H   E   A   L   H   N   H   Y   T   Q   K   S   L   S


 


2661
 CTG TCT CCC GGT TGA GGATCCCTGCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGTCGTGGAAGGTGCTACTCCA



> L   S   P   G   • (SEQ ID NO: 68)


 


2748
GTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTTTGACTAGGTGTCCTTGTATAATATTATGGGGTGGAGGCGGGTGGT


 


2840
ATGGAGCAAGGGGCAGGTTGGGAAGACAACCTGTAGGGCCTTCAGGGTCTATTGGGAACCAGGCTGGAGTGCAGTGGCACGATCTTGGCTCG


 


2932
CTGCAATCTCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGTCTCCCGAATAGTTGGGATTCCAGGCATGCACGACCAGGCTCAGCTAA


 


3024
TTTTTGTATTTTTGGTAGAGACGGGGTTTCACCATATTGGCCAGTCTGGTCTCCATCTCCTGACCTCAGGTAATCCGCCCGCCTCGGCCTCC


 


3116
CAAATTGCTGGGATTACAGGTATGAGCCACTGGGCCCTTCCCTGTCCTGTGATTTTAAAATAATTATACCAGCAGAAGGACGTCCAGACACA


 


3208
GCATGGGCTACCTGGCCATGCCCAGCCAGTTGGACATTTGAGTTGTTTGCTTGGCACTGTCCTCTCATGAATTCGTCGACAGATCTGCGCAG


 


3300
CACCATGGCCTGAAATAACCTCTGAAAGAGGAACTTGGTTAGGTACCTTCTGAGGCGGAAAGAACCAGCTGTGGAATGTGTGTCAGTTAGGG


 


3392
TGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTC


 


3484
CCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGC


 


3576 
CCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAG



 


3668
TAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTTGATTCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC



 


3760
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAAACTGGGCTTGTCGAGACAGAGAAGACTCTTGC


 


3852
GTTTCTGATAGGCACCTATTGGTCTTACTGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTAAGGC


 


3944
TAGAGTACTTAATACGACTCACTATAGGCTAGCATGGTTCGACCATTGAACTGCATCGTCGCCGTGTCCCAAAATATGGGGATTGGCAAGAA



                                > M  V  R  P  L  N  C  I  V  A  V  S  Q  N  M  G  I  G  K  N


 


4036
 CGGAGACCTACCCTGGCCTCCGCTCAGGAACGAGTTCAAGTACTTCCAAAGAATGACCACAACCTCTTCAGTGGAAGGTAAACAGAATCTGG



>  G  D  L  P  W  P  P  L  R  N  E  F  K  Y  F  Q  R  M  T  T  T  S  S  V  E  G  K  Q  N  L


 


4128
 TGATTATGGGTAGGAAAACCTGGTTCTCCATTCCTGAGAAGAATCGACCTTTAAAGGACAGAATTAATATAGTTCTCAGTAGAGAACTCAAA



>V  I  M  G  R  K  T  W  F  S  I  P  E  K  N  R  P  L  K  D  R  I  N  I  V  L  S  R  E  L  K


 


4220
 GAACCACCACGAGGAGCTCATTTTCTTGCCAAAAGTTTGGATGATGCCTTAAGACTTATTGAACAACCGGAATTGGCAAGTAAAGTAGACAT



> E  P  P  R  G  A  H  F  L  A  K  S  L  D  D  A  L  R  L  I  E  Q  P  E  L  A  S  K  V  D  M


 


4312
 GGTTTGGATAGTCGGAGGCAGTTCTGTTTACCAGGAAGCCATGAATCAACCAGGCCACCTCAGACTCTTTGTGACAAGGATCATGCAGGAAT



>  V  W  I  V  G  G  S  S  V  Y  Q  E  A  M  N  Q  P  G  H  L  R  L  F  V  T  R  I  M  Q  E


 


4404
 TTGAAAGTGACACGTTTTTCCCAGAAATTGATTTGGGGAAATATAAACTTCTCCCAGAATACCCAGGCGTCCTCTCTGAGGTCCAGGAGGAA



> F  E  S  D  T  F  F  P  E  I  D  L  G  K  Y  K  L  L  P  E  Y  P  G  V  L  S  E  V  Q  E  E


 


4496
 AAAGGCATCAAGTATAAGTTTGAAGTCTACGAGAAGAAAGACTAACTCGAGAATTCACGCGTGGTACCTCTAGAGTCGACCCGGGCGGCCGG



> K  G  I  K  Y  K  F  E  V  Y  E  K  K  D  • (SEQ ID NO: 69)


 


4588
CCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTG


 


4680
TGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGG


 


4772
AGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGATAAGGATCTGTCGACGAATTCACTGGCCGTCGTTTTA


 


4864
CAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGC


 


4956
CCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTT


 


5048
CACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCC


 


5140
TGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACC


 


5232
GAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTT


 


5324
TTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATG


 


5416
CTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTT



                          > M  S  I  Q  H  F  R  V  A  L  I  P  F  F  A  A  F  C  L  P  V  F


 


5508
 TGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTA



>  A  H  P  E  T  L  V  K  V  K  D  A  E  D  Q  L  G  A  R  V  G  Y  I  E  L  D  L  N  S  G


 


5600
 AGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGAC



>K  I  L  E  S  F  R  P  E  E  R  F  P  M  M  S  T  F  K  V  L  L  C  G  A  V  L  S  R  I  D


 


5692
 GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGG



> A  G  Q  E  Q  L  G  R  R  I  H  Y  S  Q  N  D  L  V  E  Y  S  P  V  T  E  K  H  L  T  D  G


 


5784
 CATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGG



>  M  T  V  R  E  L  C  S  A  A  I  T  M  S  D  N  T  A  A  N  L  L  L  T  T  I  G  G  P  K


 


5876
 AGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAG



>E  L  T  A  F  L  H  N  M  G  D  H  V  T  R  L  D  R  W  E  P  E  L  N  E  A  I  P  N  D  E


 


5968
 CGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAAT



> R  D  T  T  M  P  V  A  M  A  T  T  L  R  K  L  L  T  G  E  L  L  T  L  A  S  R  Q  Q  L  I


 


6060
 AGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTG



>  D  W  M  E  A  D  K  V  A  G  P  L  L  R  S  A  L  P  A  G  W  F  I  A  D  K  S  G  A  G


 


6152
 AGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACT



>E  R  G  S  R  G  I  I  A  A  L  G  P  D  G  K  P  S  R  I  V  V  I  Y  T  T  G  S  Q  A  T


 


6244
 ATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTA



> M  D  E  R  N  R  Q  I  A  E  I  G  A  S  L  I  K  H  W  • (SEQ ID NO: 70)


 


6336 
GATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTT



 


6428
CGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAA



 


6520
AAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACC


 


6612
AAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTAC


 


6704
CAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACG


 


6796
GGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCC


 


6888
CGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATC


 


6980
TTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGC


 


7072
AACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTAT


 


7164
TACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATAC


 


7256
GCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCA


 


7348
ATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACA


 


7440
ATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGC (SEQ ID NO: 71)






Example 9. Construction of Cell Line
The host cell used was a Chinese hamster ovary dihydrofolate reductase (dhfr) deficient host cell line, CHO-DG44. The DG44 host cell bank has been tested and found negative for the presence of adventitious agents prior to use. The DG44 host (CER-00-05-01) was used for the construction of cell lines expressing the anti-BDCA2.
Plasmids pJP009 and pJP010 expressing the recoded light chain and heavy chain of anti-BDCA2, respectively, were transfected into the host cell line by electroporation. Transfected cells expressing dhfr were selected using a medium deficient in a nucleosides. After selection in the αMEM nucleosides-free media described above, the transfected pool was enriched for high expressing cell lines using a combination of fluorescence activated cell sorting and the Genetix Clonepix FL instrument (CER-00-09-03). Cell colonies isolated by the ClonePix FL were picked from the semi-solid medium to 96-well plates. Individual wells were expanded and the productivity was assessed. The cell line showing the highest titer in shake flask fed-batch analysis (#49) was transferred to Research Animal Fermentation for growth in a 10 L bioreactor for generation of material for characterization.
Following the initial cell line screening, the highest producing cell lines were selected for amplification. The top cell lines were subjected to methotrexate (MTX) amplification. Amplified pools were subcloned using limiting dilution at a theoretical density of 0.5 cells per well into 384-well plates. Individual wells of 384-well plates were imaged using a Cellavista instrument (Innovatis) for the presence of a single cell per well and verified to be clonal.
The top four amplified, clonal cell lines were selected based on scale-down fed-batch shake flask and product quality analysis. Pre-Master Cell Banks (Pre-MCB) were made from these top 4 cell lines which are evaluated in bioreactors. One lead subclone was selected based on bioreactor performance and product quality analysis. A Pre-MCB vial of the lead cell line was transferred to Manufacturing for Master Cell Bank generation.
Example 10. Post-Translational Modifications of Anti-BDCA2 Antibody, BIIB059
a) Oxidation
Endo-Lys C peptide mapping of anti-BDCA2 BIIB059 antibody revealed that heavy chain Met-257, Met-433 and Trp-163 are susceptible sites to oxidation. Levels ranged from 4 to 7%. Experimental data indicate that much of the oxidation is related to sample preparation.
b) Deamidation
Analysis of the Endo-Lys-C peptide map of BIIB059 antibody showed that  ̃2.5% each of Asn-389, Asn-394 and Asn-395 in the heavy chain was deamidated (combined deamidation and succinimide formation), and that  ̃2.5% of Asn-320 in the heavy chain was deamidated (in a succinimide form). The total amount of succinimide forms for Asp-32 and Asp-34 in the light chain was  ̃3%. Combined isomerization of Asp-32 and Asp-34 in the light chain was  ̃5%. Similar to the oxidation, some of these modifications may be related to sample preparation.
c) Glycation
Glycation is a non-enzymatic modification caused by the reaction of amino groups on proteins with glucose, a component of the culture medium. Glycation is routinely detected in proteins and levels vary widely depending on cell culture conditions. In the BIIB059 antibody, the level of glycation, as measured by intact mass analysis of the non-reduced protein, was  ̃10%. Peptide mapping analysis revealed  ̃0.46% of the glycation on Lys-107 of the light chain, 0.28% on Lys-103 of the light chain and  ̃0.2% on Lys-295 of the heavy chain O-linked
d) Glycosylation
There was no detectable O-linked glycosylation of BIIB059.
e) Other Modifications (Eg. Hydroxylysine, Etc.)
Analyses showed that <1% of the heavy chain of BIIB059 antibody is in the aglycosyl form. Analysis showed no Asn-to-Ser substitutions in and there were no unknown modifications or mutations at a level of ≧1% in the antibody.
Example 11. Direct Binding of BIIB059 to the Cell Surface of Plasmacytoid Dendritic Cells
A flow cytometric whole blood assay was developed to evaluate BIIB059 binding to BDCA2 on the human or cynomolgus plasmacytoid dendritic cells (pDC). Cynomolgus monkey (Toxikon, Inc, Bedford, Mass.) or human peripheral blood (Biogen Idec) were collected in sodium heparin collection tubes and maintained at room temperature. A FACS staining antibody cocktail for identifying pDCs was added to each whole blood aliquot, incorporating CD20, CD14, CD123 and HLA-DR antibodies. Alexa647 labeled BIIB059 (Biogen Idec, Lot#17073-057) or an Alexa647-labelled hIgG isotype control was added to the FACS staining cocktail, at a concentration of 0 to 40 μg/mL. Blood was incubated on ice, protected from light, for 30 min. After 30 min., each 500 μL aliquot of whole blood (cyno) or 100 μL (human) was treated with 10 mL (cyno) or 2 mL (human) of 1× Easy Lyse Buffer (Leinco Technologies) that had been incubated at 37° C. for at least one hour. After a 10-15 min. incubation at room temperature, samples were centrifuged at 1400 rpm for 5 min. The supernatant was decanted, leaving only a pellet of white blood cells (WBC). Each WBC pellet was washed with 5 mL of FACS buffer (1% BSA+0.002% NaAzide+1 mM CaCl2+1 mM MgCl2 in PBS), and centrifuged at 1400 rpm for 5 min. The supernatant was decanted, and each WBC pellet was resuspended in 200 μL of FACS buffer and transferred to a 96-well round bottom plate (Fisher Scientific). The plate was centrifuged at 1400 rpm for 5 min. The supernatant was dumped out of the plate, and each WBC pellet was washed with 200 μL of FACS buffer. The plate was centrifuged at 1400 rpm for 5 min, and the supernatant dumped out of the plate. Following washing (as above), WBCs were resuspended in 200 μL of 1% paraformaldehyde (PFA) in PBS, and fixed at 4° C. overnight, protected from light. Immediately prior to flow cytometry analysis, WBCs were filtered using a 60-micron nylon mesh filter plate (Millipore). Each pellet was then transferred to a new, 96-well round bottom plate and centrifuged at 1400 rpm for 5 min. Each WBC pellet was resuspended in 250 μL of FACS buffer and fluorescence intensity measured on a LSRII 4-color FACS machine. Single color compensation was acquired using anti-mouse Ig Compensation Particle beads set (BD Biosciences). Analysis was performed using FlowJo and GraphPad Prism software. BIIB059 bound cynomolgus and human cells similarly with EC50 values of 1-2 μg/mL (7-13 nM) (FIG. 6).
Example 12. Assessing Self Association for BIIB059
The AlphaScreen assay is a homogeneous proximity assay utilizing glutathione donor and acceptor beads (Perkin Elmer) to bind human FcRIIa (CD32a) GST. Various concentrations of the antibodies to be tested were added in this mixture. Since the binding of the antibody to FcRIIa is monovalent, the only way for a signal to be generated is if donor and acceptor beads both have a bound antibody which then associates bringing the beads within 200 nm allowing for the production of singlet oxygen and consequent light emission. The level of emission detected by the Envision (Perkin Elmer) instrument is proportional to the degree of self-association.
FIG. 7 shows the results of the Alpha Screen for BIIB059 compared to 5c8 (negative control) and LT105 (positive control with strong self-association).
Example 13. Assessing Non-Specific Binding of BIIB059
Cross-interaction chromatography (CIC) is a high throughput method for preliminary assessment of the stickiness of mAb candidates (Jacobs et al., Pharm Res., 27(1):65-71 (2010)). In this method, bulk polyclonal human IgG is chemically coupled to an NHS-activated chromatography resin. The retention times of BIIB059 on non-derivatized and IgG-derivatized columns were then compared to a control panel of well-behaved and poorly behaved mAbs. BIIB059 showed no evidence of non-specific binding by this method as evidenced by its low retention times and K′ values.
CIC Data Showing Solubility and Non-Specific Binding


















Rt-
Rt-





Antibody
Solubility
Test
Blank
K′























5C8
good
9.3
9.46
−0.017




Hu H0/L0
bad
14.1
10.4
0.356
←
higher K′ values


Li33
bad
10.8
9.2
0.174
←
may indicate


Herceptin
good
9.5
9.4
0.011

lower solubility


15F3 H4/
good
9.3
9.2
0.011


L1 (1-3)


24F4 H4/
good
9.3
9.1
0.022


L1 (1-5)


16A8
good
9.1
9
0.011









Example 14. Assessing Stability of BIIB059
Differential scanning fluorometry was used to test the stability of BIIB059 over a range of buffer conditions for the initial research formulation. Protein unfolding was monitored on an Mx3005p real-time PCR system (Agilent Technologies) in a 96-well format using 10 μg of protein in 50 μL PBS (at pH 7.0) supplemented with SYPRO orange fluorophor at a final concentration of 10× (based on Invitrogen stock designation of 1000×). Samples were heated from 25° C. to 95° C. at 1° C./min with fluorescence intensity measured three times every 1° C. Fluorescence intensities were plotted as a function of temperature. Tm were derived from these curves by taking the negative derivative (“—R′(T)” in the Mx3005p software) and selecting the local minima of the derivative plots. Using a base buffer of 20 mM sodium citrate, the pH was varied from 5.0 to 7.5 and NaCl and sucrose concentrations were varied from 50 to 250 mM.
Stability was similar throughout these buffer ranges. FIG. 8 shows data with 150 mM NaCl and 250 mM sucrose as a function of pH. 20 mM sodium citrate, 150 mM NaCl pH 6.0 was chosen as the research formulation over sucrose due to difficulty reaching high concentrations with sucrose using research centrifugal concentrators.
Example 15. Assessing Agitation Stability of BIIB059
A 0.2 mL volume of the BIIB059 mAb solution at 1 mg/mL in 20 mM sodium citrate, pH 6.0, 150 mM NaCl was subjected to reciprocal shaking at room temperature in 2 mL glass vials (Waters, WAT270946C) using a Lab-Line Instruments model 4626 Titer Plate Shaker set at 600 rpm. Aggregation was assessed by monitoring increases in turbidity at 320 nm using a Beckman DU640 spectrophotometer. BIIB059 displayed time-dependent aggregation. Normally wild type human IgG1 antibodies do not aggregate under these agitation conditions. As shown in FIG. 9, aggregation was completely suppressed by the addition of 0.03% Tween 80, a common formulation excipient. Agitation-induced aggregation can sometimes be highly pH dependent. The aglycosyl IgG4/IgG1 showed a more rapid and more extensive aggregation than BIIB059. Aggregation of aglycosyl IgG4/IgG1 was also suppressed with addition of Tween 80.
Example 16. Assessing Viscosity of BIIB059
The stability and viscosity of BIIB059 samples were measured at high concentrations of 150 mg/mL and greater, to support potential development of the product for subcutaneous administration. Solutions of BIIB059 were centrifuged in ultra-concentrator tubes to limit volumes and the concentrations achieved were determined by UV scans. Stability was determined by size exclusion chromatography after storage at 2-8° C. for one and two weeks. Protein concentrations of greater than 200 mg/mL were readily achieved for small amounts of protein in 20 mM citrate, pH 6, 150 mM NaCl buffer and aggregate remained low (0.68%) after two weeks at 2-8° C. Viscosity was measured using a Viscopro2000 instrument (Cambridge Viscosity). The viscosity at 150 mg/mL was only 8 cP in the citrate/saline buffer. These results demonstrate that a high-concentration formulation of BIIB059 should be achievable.
Example 17. Cloning the Human BDCA2 Gene
The full-length human BDCA2 (huBDCA2) cDNA was subcloned in Invitrogen's pCR4TOPO cloning vector from Open Biosystems: this plasmid was designated pEAG2367. DNA sequencing confirmed that its cDNA was identical to the full-length human BDCA2 cDNA in the reference GenBank® accession number NM_130441. The huBDCA2 full-length open reading frame encoded by pEAG2420 is shown below, with the TM-HMM-predicted transmembrane domain underlined:








(SEQ ID NO: 1)










1
MVPEEEPQDR EKGLWWFQLK VWSMAVVSIL LLSVCFTVSS VVPHNFMYSK



 


51
TVKRLSKLRE YQQYHPSLTC VMEGKDIEDW SCCPTPWTSF QSSCYFISTG


 


101
MQSWTKSQKN CSVMGADLVV INTREEQDFI IQNLKRNSSY FLGLSDPGGR


 


151
RHWQWVDQTP YNENVTFWHS GEPNNLDERC AIINFRSSEE WGWNDIHCHV


 


201
PQKSICKMKK IYI*






The huFcεRIγ full-length open reading frame encoded by pEAG2413, which is identical to the reference sequence in GenBank® accession number NP_004097, is shown below:








(SEQ ID NO: 2)










1
MIPAVVLLLL LLVEQAAALG EPQLCYILDA ILFLYGIVLT LLYCRLKIQV



 


51  
RKAAITSYEK SDGVYTGLST RNQETYETLK HEKPPQ*







A CHO expression vector co-expressing both human BDCA2 and FcεRIγ cDNAs in tandem transcriptional units was constructed by subcloning the 2.11 kb SpeI fragment from pEAG2413 into the linearized, phosphatased 6.71 kb SpeI vector backbone of pEAG2420, resulting in a “univector” designated pEAG2456. The human BDCA2 and FcεRIγ cDNAs in pEAG2420 were sequence confirmed. A stable CHO cell line stably co-expressing BDCA2 and FcεRIγ cDNAs was produced by transfection with pEAG2456.
Example 18. Cloning the Cyno and Rhesus BDCA2 Gene
The deduced macaque BDCA2 open reading frame encoded by pEAG2384 and one of the SNP forms observed in pEAG2383 is shown below. This SNP form is referred to below as the E73 SNP form of cyno BDCA2. In the rhesus, a single sequence identical to the E73 SNP form of cyno BDCA2 was observed.








(SEQ ID NO: 72)










 1
MVPEEEPQDR EKGVWWFQLK VWSVAVVSIL LLCVCFTVSS VASHNFMYSK



 


 51






101
MQSWTKSQNN CSVMGADLVV INTKEEQDFI TQNLKINSAY FLGLSDPKGW


 


151
RHWQWVDQTP YNKNVTFWHS GEPNSPDERC AIINFRSEEW GWNDVHCHVP


 


201
QKSICKMKKI YI* 






In a second SNP form of cyno BDCA2, residue 73 (GAA=Glu, E) highlighted above is Lysine (AAA=Lys, K). This second SNP form is referred to as the K73 SNP form of cynomolgus monkey BDCA2. In human BDCA2, residue 73 is Glutamic acid. The gapped alignment of the human (upper) and macaque (lower) BDCA2 sequences, which share 90.6% identity, is shown below. Potential N-linked glycosylation sites are shaded. Macaque BDCA2 lacks one potential N-linked glycosylation site present in human (NSS at 137-139 in human vs. NSA in macaque).



















A consensus cynomolgus monkey FcεRIγ open reading frame is shown below:








(SEQ ID NO: 73)










1
MIPAVVLLLL LLVEQAAALG EPQLCYILDA ILFLYGIVLT LLYCRLKIQV



 


51
RKAAIASYEK SDGVYTGLST RNQETYETLK HEKPPQ 






The cynomolgus monkey FcεRIγ cDNA sequence is a perfect match to that of the predicted rhesus cDNA (based upon genomic short reads) described in GenBank® accession number XM_001115585 and a cyno sequence deposited as GenBank® accession number AF485816 by scientists at Genentech. The cyno FcεRIγ protein sequence shares 98.9% identity with human FcεRIγ protein, differing by only a single, conservative substitution. The alignment between human (upper) and cyno (lower) FcεRIγ is shown below:









 1 MIPAVVLLLLLLVEQAAALGEPQLCYILDAILFLYGIVLTLLYCRLKIQV 50




   ||||||||||||||||||||||||||||||||||||||||||||||||||



 1 MIPAVVLLLLLLVEQAAALGEPQLCYILDAILFLYGIVLTLLYCRLKIQV 50



 


            .         .         .




51 RKAAITSYEKSDGVYTGLSTRNQETYETLKHEKPPQ* 87 
(SEQ ID NO: 2)


   ||||| |||||||||||||||||||||||||||||||



51 RKAAIASYEKSDGVYTGLSTRNQETYETLKHEKPPQ* 87 
(SEQ ID NO: 73)






A CHO expression vector co-expressing both the cyno E73 SNP form of BDCA2 and FcεRIγ cDNAs in tandem transcriptional units was constructed by subcloning the 2.11 kb SpeI fragment from pCN652 into the linearized, phosphatased 6.72 kb SpeI vector backbone of pCN654, resulting in a “univector” designated pEAG2668. The cyno BDCA2 and FcεRIγcDNAs in pEAG2668 were sequence confirmed. A stable CHO cell line stably co-expressing BDCA2 and FcεRIγ cDNAs was produced by transfection with pEAG2668.
Example 19. Cross-Reactivity Between Human and Cyno BDCA2
To determine whether the cynomolgus monkey E73/K73 BDCA2 SNP affected anti-BDCA2 binding, 293E cells were co-transfected with expression vectors carrying an EGFP reporter (pEAG1458) and BDCA2 and FcεRIγ cDNAs (human BDCA2: pEAG2420 and FcεRIγ: pEAG2413; cyno E73 BDCA2: pCN652 or K73 BDCA2: pCN656 and cyno FcεRIγ: pCN652) at 1:1:1 molar ratios. At 3 days post-transfection, cells were harvested and stained with PE-conjugated Miltenyi anti-human BDCA2 AC144 mAb (Miltenyi Biotec catalog number 130-090-511) in a direct binding dilution titration FACS, gating on green EGFP-positive cells. FIG. 10 shows the direct binding of AC144 to human and cyno surface BDCA2.
The apparent EC50's are essentially equivalent for human BDCA2 and both E73 and K73 SNP forms of cynomolgus monkey BDCA2. Given this result, CHO stable transfectants for surface full-length BDCA2 were generated using the human BDCA2/FcεRIγ expression vector pEAG2456 and the cyno E73 SNP BDCA2/FcεRIγ expression vector pEAG2668. These lines were used for triage of human/cyno cross-reactive anti-BDCA2 antibodies.
Example 20. Fc Fusion Constructs of Human and Cynomolgus BDCA2 Ectodomains
Five Fc fusion constructs of human and cyno BDCA2 ECD were engineered. In three of the constructs, BDCA2 is attached via a G4S linker sequence to the C-terminus of human IgG1 hinge and Fc. In two of the constructs, the G4S linker was replaced with a TEV protease cleavage site ENLYFQC.
As BDCA2 is a type II membrane protein (the C-terminus is outside the cell), the design of soluble Fc fusion proteins involved adding the C-terminal ectodomain of BDCA2 (residues 45-213 for human BDCA2) to the C-terminus of engineered IgG Fc's with secretion was driven by an in-frame murine kappa light chain signal sequence. The full-length huBDCA2 construct pEAG2367 was used as template for PCR with primers 5′ CAG TGT CTG TTT CAC TCC CGG GGG TGG CGG TGG TAG CAA TTT TAT GTA TAG C 3′ (SEQ ID NO:74) (to add a 5′ XmaI (Pro-Gly) and Gly4Ser linker immediately before the huBDCA2 ectodomain's 5′ end) and 5′ CCA GGG AGA ATA GGA TCC TTA TAT GTA GAT CTT 3′ (SEQ ID NO:75) (to add a 3′ BamHI site immediately after the huBDCA2 terminator). The 0.56 kb PCR product was purified and subcloned into Invitrogen's pCRBluntIITOPO cloning vector, producing pEAG2417, whose insert cDNA sequence was confirmed. The 0.53 kb XmaI-BamHI fragment from pEAG2417 and the 0.75 kb NotI-XmaI fragment from pEAG1397 (carrying an engineered huIgG1 Fc whose secretion is driven by an in-frame engineered murine kappa light chain signal sequence) were ligated with the 1.89 kb BamHI-XbaI and 4.17 kb XbaI-NotI vector backbone fragments from the expression vector pV90, producing the huIgG1 Fc-huBDCA2 fusion protein expression vector pEAG2421, whose cDNA insert sequence was confirmed. The deduced open reading frame encoded by pEAG2421 is shown below:








(SEQ ID NO: 76)










1
MKLPVRLLVL MFWIPASSSE PKSSDKTHTC PPCPAPELLG GPSVFLFPPK



 


51
PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY


 


101
NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP


 


151
QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP


 


201
VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG


 


251
GGGGSNFMYS KTVKRLSKLR EYQQYHPSLT CVMEGKDIED WSCCPTPWTS


 


301
FQSSCYFIST GMQSWTKSQK NCSVMGADLV VINTREEQDF IIQNLKRNSS


 


351
YFLGLSDPGG RRHWQWVDQT PYNENVTFWH SGEPNNLDER CAIINFRSSE


 


401
EWGWNDIHCH VPQKSICKMK KIYI*


kappa light chain signal sequence: residues 1-19 above (italicized)


human IgG1 Fc: residues 20-250 above


G4S linker: residues 251-255 above (boldened)


huBDCA2 ectodomain: residues 256-424 above (underlined)






To construct an expression vector for a muIgG2a Fc-huBDCA2 fusion protein, the 0.53 kb XmaI-BamHI fragment from pEAG2417 and the 0.75 kb NotI-XmaI fragment from pEAG1442 (carrying an engineered murine IgG2a Fc whose secretion is driven by an in-frame engineered murine kappa light chain signal sequence) were ligated with the 1.89 kb BamHI-XbaI and 4.17 kb XbaI-NotI vector backbone fragments from the expression vector pV90, producing pEAG2423, whose cDNA insert sequence was confirmed. The deduced open reading frame encoded by pEAG2423 is shown below:








(SEQ ID NO: 77 )










1
MKLPVRLLVL MFWIPASSSE PRGPTIKPSP PCKCPAPNLL GGPSVFIFPP



 


51
KIKDVLMISL SPIVTCVVVD VSEDDPDVQI SWFVNNVEVH TAQTQTHRED


 


101
YNSTLRVVSA LPIQHQDWMS GKEFKCKVNN KDLPAPIERT ISKPKGSVRA


 


151
PQVYVLPPPE EEMTKKQVTL TCMVTDFMPE DIYVEWTNNG KTELNYKNTE


 


201
PVLDSDGSYF MYSKLRVEKK NWVERNSYSC SVVHEGLHNH HTTKSFSRTP


 


251
GGGGGSNFMY SKTVKRLSKL REYQQYHPSL TCVMEGKDIE DWSCCPTPWT


 


301
SFQSSCYFIS TGMQSWTKSQ KNCSVMGADL VVINTREEQD FIIQNLKRNS


 


351
SYFLGLSDPG GRRHWQWVDQ TPYNENVTFW HSGEPNNLDE RCAIINFRSS


 


401
EEWGWNDIHC HVPQKSICKM KKIYI* 


kappa light chain signal sequence: residues 1-19 above (italicized)


murine IgG2a Fc: residues 20-251 above


G4S linker: residues 252-256 above (boldened)


huBDCA2 ectodomain: residues 257-425 above (underlined)






Stable CHO cell lines producing the Fc-huBDCA2 fusion proteins were produced by transfection with expression vectors pEAG2421 and pEAG2423. These fusion proteins were used in ELISA and Octet binding assays for antibody triage during candidate screening.
To engineer cynomolgus (cyno) BDCA2 to make an Fc fusion protein protein, the full-length E73 SNP variant of cyno BDCA2 in construct pCN648 was subjected to site-directed mutagenesis with primers 5′ CTC TGT GTC TGT TTC ACT CCC GGG GGT GGC GGT GGT AGC AAT TTT ATG TAT AGC 3′ (SEQ ID NO:78) and its reverse complement, to add a 5′ XmaI (Pro-Gly) and Gly4Ser linker immediately before the huBDCA2 ectodomain's 5′ end, producing construct pEAG2675, whose cDNA insert sequence was confirmed. To construct an expression vector for a muIgG2a Fc-cyno BDCA2 fusion protein, the 0.53 kb XmaI-BamHI fragment from pEAG2675 and the 0.75 kb NotI-XmaI fragment from pEAG1442 (carrying an engineered murine IgG2a Fc whose secretion is driven by an in-frame engineered murine kappa light chain signal sequence) were ligated with the 1.89 kb BamHI-XbaI and 4.17 kb XbaI-NotI vector backbone fragments from the expression vector pV90, producing pEAG2677, whose cDNA insert sequence was confirmed. The deduced open reading frame encoded by pEAG2677 is shown below:








(SEQ ID NO: 79)










1
MKLPVRLLVL MFWIPASSSE PRGPTIKPSP PCKCPAPNLL GGPSVFIFPP



 


51
KIKDVLMISL SPIVTCVVVD VSEDDPDVQI SWFVNNVEVH TAQTQTHRED


 


101
YNSTLRVVSA LPIQHQDWMS GKEFKCKVNN KDLPAPIERT ISKPKGSVRA


 


151
PQVYVLPPPE EEMTKKQVTL TCMVTDFMPE DIYVEWTNNG KTELNYKNTE


 


201
PVLDSDGSYF MYSKLRVEKK NWVERNSYSC SVVHEGLHNH HTTKSFSRTP


 


251
GGGGGSNFMY SKTVKRLSKL QEYQQYYPSL TCVMEGKDME DWSCCPTPWT


 


301
SFQSSCYFIS TVMQSWTKSQ NNCSVMGADL VVINTKEEQD FITQNLKINS


 


351
AYFLGLSDPK GWRHWQWVDQ TPYNKNVTFW HSGEPNSPDE RCAIINFRSE


 


401
EWGWNDVHCH VPQKSICKMK KIYI*


kappa light chain signal sequence: residues 1-19 above (italicized)


murine IgG2a Fc: residues 20-251 above


G4S linker: residues 252-256 above (boldened)


cyno BDCA2 ectodomain: residues 257-424 above (underlined)






A stable CHO cell line producing the Fc-cyno BDCA2 fusion protein was produced by transfection with expression vector pEAG2677.
The muIgG2a Fc-BDCA2 fusion proteins were subjected to limited proteolysis, to isolate monomeric BDCA2 ectodomain proteins. To facilitate isolation of recombinant soluble BDCA2 ectodomain, new Fc fusion constructs were constructed in which a TEV protease cleavage site was inserted between the C-terminus of the Fc and the N-terminus of the BDCA2 ectodomain. Syngenes carrying engineered human or cyno BDCA2 ectodomains with a 5′ XmaI site (Pro-Gly) for fusion to the Fc C-terminus followed by an in-frame TEV cleavage site (ENLYFQG) fused to residue 45 of the BDCA2 sequence and a 3′ BamHI site following the BDCA2 terminator were designed and delivered by GeneWiz as XmaI-BamHI insert's in their proprietary pUC57-amp cloning vector. The sequences of the inserts in engineered XmaI-BamHI TEV-BDCA2 ectodomain cDNA constructs, pEAG2917 (human) and pEAG2918 (cyno), were confirmed. To construct pV90-IRES-dhfr-based CHO expression vectors for huIgG1 Fc-TEV-BDCA2 fusion proteins, the 0.75 kb NotI-XmaI fragment of pEAG1397 and the 0.54 kb XmaI-BamHI fragments from either pEAG2917 or pEAG2918 were subcloned into the 5.4 kb BglII-NotI vector backbone fragment of pXJC194, producing pEAG2937 (Fc-huBDCA2) or pEAG2938 (Fc-cyno BDCA2). The insert cDNAs in pEAG2937 and pEAG2938 were sequence confirmed. Stable CHO cell lines were generated by transfection with pEAG2937 and pEAG2938. The deduced open reading frame of the huFc-TEV-huBDCA2 fusion protein encoded by pEAG2937 is shown below:








(SEQ ID NO: 80)










1
MKLPVRLLVL MFWIPASSSE PKSSDKTHTC PPCPAPELLG GPSVFLFPPK



 


51
PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY


 


101
NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP


 


151
QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP


 


201
VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG


 


251
ENLYFQGNFM YSKTVKRLSK LREYQQYHPS LTCVMEGKDI EDWSCCPTPW


 


301
TSFQSSCYFI STGMQSWTKS QKNCSVMGAD LVVINTREEQ DFIIQNLKRN


 


351
SSYFLGLSDP GGRRHWQWVD QTPYNENVTF WHSGEPNNLD ERCAIINFRS


 


401
SEEWGWNDIH CHVPQKSICK MKKIYI* 


kappa light chain signal sequence: residues 1-19 above (italicized)


human IgG1 Fc: residues 20-250 above


TEV cleavage site: residues 251-257 above (boldened)


huBDCA2 ectodomain: residues 258-426 above






The deduced open reading frame of the huFc-TEV-cyno BDCA2 fusion protein encoded by pEAG2938 is shown below:










(SEQ ID NO: 81)



1
MKLPVRLLVL MFWIPASSSE PKSSDKTHTC PPCPAPELLG GPSVFLFPPK



 


51
PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY


 


101
NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP


 


151
QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP


 


201
VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG


 


251
ENLYFQGNFM YSKTVKRLSK LQEYQQYYPS LTCVMEGKDM EDWSCCPTPW


 


301
TSFQSSCYFI STVMQSWTKS QNNCSVMGAD LVVINTKEEQ DFITQNLKIN


 


351
SAYFLGLSDP KGWRHWQWVD QTPYNKNVTF WHSGEPNSPD ERCAIINFRS


 


401
EEWGWNDVHC HVPQKSICKM KKIYI* 


kappa light chain signal sequence: residues 1-19 above (italicized)


human IgG1 Fc: residues 20-250 above


TEV cleavage site: residues 251-257 above (boldened)


cyno BDCA2 ectodomain: residues 258-425 above (underlined)






Example 21. BIIB059 Binding to BDCA2-Fc Fusion Proteins
The ability of BIIB059 to bind huBDCA2-Fc in solution was assessed by SEC (FIG. 11). When analyzed alone, BIIB059 (top panel) and huBDCA2 (middle panel) eluted as single sharp peaks with molecular masses of  ̃150 kDa. When BIIB059 and huBDCA2-Fc were mixed together and analyzed (bottom panel), there was a shift of BIIB059 and huBDCA2-Fc to higher masses of >550 kDa as evident from their elution at earlier positions in the chromatogram. The heterogeneity in the elution peak is presumably caused by the fact that both BIIIB059 and BDCA2-Fc each contain 2 binding sites and consequently a large number of complexes with different stoichiometries of BIIB059 and BDCA2 are formed.
The binding of cynoBDCA2 ECD to BIIB059 was also assessed by SEC and similarly led to a quantitative shift to higher molecular mass complexes.
Example 22. Calcium Enhances the Binding of BIIB059 to BDCA2
The binding of BIIB059 to human BDCA2 fused to murine Fc (huBDCA2-muFc) in the presence of calcium or EDTA was studied in an Octet binding assay. The huBDCA2-muFc protein was captured on an anti-murine Fc biosensor, followed by the association of BIIB059 and the dissociation step. All steps were run in 50 mM HEPES, pH 7, 100 mM NaCl, 1 mg/ml BSA, 0.02% Tween 20 and 0.001% azide containing either 10 mM CaCl2 or 10 mM EDTA.
FIG. 12 shows that BIIB059 binding is enhanced by the addition of calcium relative to EDTA leading to about a 2-fold higher signal. Both association and dissociation rates were affected by calcium.
Example 23. Binding Measurements
Octet was used to monitor binding of BIIB059 to the BDCA2-Fc fusion protein and BDCA2 ECD. FIG. 13 shows an Octet experiment in which BIIB059 was loaded onto anti-human Fc Octet tips at a concentration of 20 μg/mL. For the association step, human and cynomolgus BDCA2 ECD was added at a concentration of 2 μg/mL. The buffer for this experiment was 50 mM HEPES, pH 7, 100 mM NaCl, 5 mM CaCl2, 1 mg/mL BSA, 0.02% Tween 20 and 0.001% azide. Under these conditions, binding of BIIB059 to human and cyno BDCA2 ECD was comparable
Example 24. PBMC Assay to Determine IC50 Value for BIIB059 for Inhibition of TLR9-Induced IFNα Production
BDCA2 ligation has been shown to activate a BCR-like signaling cascade, which potently suppresses the ability of pDCs to produce type I IFNs and other cytokines in response to TLR ligands (Cao W. et al., PLoS Biol., 5(10):e248 (2007)) Inhibition of TLR9-induced IFNα production by PBMC was used as the primary cellular assay for screening.
PBMCs from heparinized venous blood of healthy donors were isolated by discontinuous gradient centrifugation over Ficoll, washed in PBS and re-suspended in complete culture medium (RPMI with 3% FBS). 1×106 cells were seeded/well and stimulated with 10 μg/mL of the TLR9 ligand (CpG-A ODN 2216) in the presence of doses of BIIB059 and 24F4A-Agly (an Fc crippled version of BIIB059), or isotype control mAb ranging from 10 μg/mL to 1 pg/mL in a total assay volume of 200 μL/well. The plates were incubated overnight (18 hours) at 37° C., and the supernatants were collected for evaluation in IFNα ELISA assays (PBL InterferonSource). The assays were performed according to the manufacturer's protocol. The titrations of BIIB059 and 24F4A agly were tested to determine the IC50 for inhibition of TLR9-induced IFNα production. A total of twelve independent experiments gave an average IC50 of 0.001 μg/mL for BIIB059. The Aglycosylated mAb was less potent, with an average IC50 of 0.007 μg/mL (FIG. 14).
The ability of anti-BDCA2 mAb to inhibit IFNα production following stimulation with a physiologically relevant ligand, namely, sera from patients with SLE was also tested. SLE sera are believed to induce type I IFN through complexes of anti-DNA autoantibodies and immunostimulatory hypomethylated DNA that stimulate TLR9. PBMCs were stimulated with sera from an SLE patient (provided by Dr. Gregg Silverman, NYU) and used at a final dilution of 1/5. Antibody 24F4S H4/L1C95S, which differs from BIIB059 by 1 amino acid residue, completely abrogated IFNα production from SLE sera stimulated pDCs (FIG. 18).
Example 25. TLR9-Induced IFNα Production in Whole Blood Assay
The activity of BIIB059 was also evaluated in a whole blood assay of TLR9-induced IFNα production.
Whole blood was drawn from heparinized venous blood of healthy donors. Doses of BIIB059 and 24F4A-Agly ranged from 10 μg/mL to 1 pg/mL in a total assay volume of 200 μl/well. CpG-A was added at 200 μg/mL, which was determined to be optimal for stimulation of IFNα production in whole blood. Plates were incubated for 18 hours at 37° C. and supernatants collected for use in IFNα ELISA assays (PBL InterferonSource). The assays were performed according to the manufacturer's protocol. Shown in FIG. 15A is a representative experiment of 6 independent experiments performed. The inhibitory potency of BIIB059 in the TLR9-induced IFNα assay in whole blood was similar to the potency seen in the PBMC assays. In addition to inhibiting pDC-derived cytokines (IFNα, IL-6), BIIB059 treatment also led to inhibition of a large array of cytokines and chemokines (FIG. 15C).
The following experiment was performed to determine if BIIB059 could inhibit TLR9-induced IFNα production in whole blood from SLE patients similarly to healthy volunteers. To this end, whole blood from 2 SLE patients or 2 healthy controls was stimulated with 200 μg/ml CpGA in the presence of 10 μg/ml BIIB059 and IFNα production was assessed by ELISA. Specifically, whole blood from 2 SLE patients or 2 healthy donors was provided by Bioreclamation LLC by overnight shipping. Upon arrival, blood was treated with 10 μg/mL BIIB059 or isotype control and stimulated with 200 μg/mL CpG-A and plated in 96 well plate. Plates were incubated for 18 hours at 37° C. and supernatants collected for use in IFNα ELISA assays (PBL InterferonSource). The assays were performed according to the manufacturer's protocol.
As shown in FIG. 15B, BIIB059 showed similar potency in whole blood from SLE patients as compared to healthy volunteers.
Example 26. Assessing BIIB059-Mediated Inhibition of Type I Interferons
The inhibitory activity of BIIB059 was also confirmed using purified pDCs stimulated with either synthetic TLR agonists (CpG-A) or the more physiologic stimulus (SLE sera). The inhibitory effect of BDCA2 cross-linking on other pDC derived cytokines (IL-6) was also determined. BIIB059 activity was confirmed using a variety of approaches such as qualitative polymerase chain reaction and ELISA.
a) Q-PCR
Thirteen IFNα subtypes and a single member of IFNβ exist in humans. Stimulation with TLR9 agonist results in upregulation of most type I IFNs (Ito T. et al., Blood, 107(6):2423-31 (2006)) Inhibition of individual type I IFN genes was evaluated using qualitative polymerase chain reaction (Q-PCR) assays.
pDCs were purified using a two-step magnetic bead separation procedure (MACS kit, Miltenyi Biotec). 5×104 pDCs/well were stimulated with 5 μM CPG-A in the presence or absence of increasing concentrations of BIIB059 or 10 μg/mL of isotype control. Total assay volume was 200 μl/well. Plates were incubated for 18 hours at 37° C., and RNA was extracted from cells using Trizol reagent (Invitrogen corporation) and further purified using an RNeasy mini column (Qiagen Sciences). All primers and probes were purchased from Applied Biosystems Inc. Relative transcript quantities were determined for each sample by comparison to the oligonucleotide standard curve using Sequence Detection Software (Applied Biosystems Inc.) and normalized to a control (GAPDH).
Treatment with BIIB059 inhibited transcription of all type I IFNs tested, thereby recapitulating previous data using anti-BDCA2 antibody clone AC144 (Cao W. et al., PLoS Biol., 5(10):e248 (2007)).
b) ELISA
The effect of BIIB059 on inhibition of pDC cytokines was tested at the protein level using ELISA. 5×104 purified pDCs/well were stimulated with 5 μM CPG-A in the presence or absence of increasing concentrations of BIIB059 or 10 μg/mL of isotype control. Shown in FIG. 17 are the amounts of secreted IFNα and IL-6 measured from a representative donor of three tested healthy donors.
BDCA2 ligation with BIIB059 potently inhibited IFNα production and greatly reduced the production of IL-6 induced by CpG-A stimulation.
Example 27. BIIB059-Mediated Receptor Internalization
Ligation of BDCA2 with anti-BDCA2 mAb (clone AC144, Miltenyi) has been shown to rapidly induce receptor internalization (Dzionek A. et al., J. Immunol., 165(11):6037-46 (2000)). The following experiment was directed at determining the kinetics of BIIB059-mediated BDCA2 internalization.
Human whole blood was treated with 10, 1, 0.1 or 0.01 μg/mL of BIIB059 or an isotype control (10 μg/ml) at 37° C. for the periods indicated and then incubated for 30′ at 4° C. with FITC-labeled non-cross blocking anti-BDCA2 mAb (clone 2D6), anti-HLADR, anti-CD123, anti-CD14 and anti-CD20. Red blood cells (RBCs) were lysed using 1× Easy-lyse buffer (BD Bioscience) and the remaining cells fixed. Shown in FIG. 19A are mean fluorescence intensity (MFI) values of 2D6-FITC staining of gated CD14−CD20−HLA-DR+CD123+pDCs. FMO (fluorescence minus one control) consisted of the FACS staining cocktail minus 2D6-FITC. The data in this figure is a representative experiment of 3 independent experiments performed.
As shown in FIG. 19A, upon incubation with BIIB059 at 1 μg/ml, the intensity of FITC-labeled 2D6 staining rapidly decreased reaching background levels within one hour of incubation at 37° C. Tenfold lower BIIB059 concentration (0.1 μg/ml) affected the kinetics of endocytosis delaying it by 2 hours. This demonstrates that BDCA2 is internalized upon ligation with BIIB059 with dose dependent kinetics.
The following experiment was conducted to ascertain whether BIIB059-mediated receptor internalization affected IFN inhibition. Whole blood was collected from heparinized venous blood of healthy donors and pre-incubated with BIIB059 (to allow for receptor internalization) or isotope for the duration indicated. At each time point after pre-incubation, cells were challenged with 200 μg/mL CpGA and incubated for an additional 18 hours at 37° C. Supernatants were collected for use in IFNα ELISA assays (PBL InterferonSource). The assays were performed according to the manufacturer's protocol. FIG. 19B is a representative experiment of 3 independent experiments performed. As shown in FIG. 19B, after 9 hours preincubation with BIIB059 prior to stimulation—corresponding to maximal internalization—did not affect IFN inhibition suggesting that BDCA2 endocytosis and TLR9 inhibition are potentially linked. To test this hypothesis anti-BDCA2 mAbs were used that were incapable of mediating IFN inhibition and demonstrated lack of internalization. In addition, we have previously shown that bivalent binding was necessary for anti-BDCA2 mediated IFN inhibition. In fact, Fab fragment did not lead to internalization or IFN inhibition. Taken together, these data raise the possibility that BDCA2 mediated TLR9 inhibition requires endocytosis and localization into endosomal compartments containing TLR9. This hypothesis can be tested using live imaging to track BDCA2 internalization and trafficking in the cell after BIIB059 ligation.
Example 28. Antibody Effector Function
The Fc domain of BIIB059 is a fully glycosylated human IgG1, and is competent to bind both cellular Fcγ receptors and complement, and to induce cellular effector immune cell responses, both through antigen-dependent cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). In order to confirm the binding of BIIB059 to Fc receptors, relative binding affinities were measured using the Amplified Luminescent Proximity Homogeneous Assay (ALPHA) technology from Perkin Elmer (FIG. 20). The assay was performed in a competitive format in which serial dilutions of test antibodies were incubated with the receptor-GST fusion proteins and anti-GST acceptor beads overnight at 4° C. in a 96-well plate. Streptavidin donor beads and biotinylated wild-type IgG1 were also incubated overnight at 4° C. in a separate tube and then added to the assay plate the next day. The plates were incubated at room temperature for 2 hr with gentle shaking and read in an Envision plate reader (Perkin Elmer). The data were plotted to a 4-parameter curve fit using Graphpad Prism software to calculate the IC50 values in order to determine the relative binding affinities. IC50 values of BIIB059 for FcγR1: 0.03 μg/mL, FcγR11a: 11 μg/mL FcγR11b: 17 μg/mL FcγR111a: and 3 μg/mL were calculated. These values are in line with those observed for other human IgG1 antibodies in this assay. IC50 values for the G4P/G1 agly low effector function version of 24F4 used in the cyno were studies were also determined. As expected, no binding was detected to FcγR11a, FcγR11b, and FcγR111a and binding to FcγR1 was reduced by 100-fold. The 5c8 antibody both in IgG1 WT and G4P/G1 agly frameworks were included in the assays as comparators.
Example 29. Complement Fixation
Antibody coating of targets has been shown to mediate potent killing mechanisms via ADCC or CDC. These effector functions of antibodies are mediated by the antibody Fc region. This experiment was directed to testing the ability of BIIB059 to recruit complement by testing its binding to C1q by ELISA.
The C1q binding assay was conducted in a 96 well ELISA format using Maxisorb ELISA plates. The test antibody was coated in a 3-fold dilution series in PBS starting at 15 μg/mL overnight at 2-8° C. and the wells were then washed with PBS, 0.05% Tween 20 and blocked with 200 μl of 0.1 M Na Phosphate pH 7.2, 0.1 M NaCl, 0.1% gelatin, 0.05% Tween 20. Subsequently, 50 μl/well of 2 μg/mL of human C1q from Complement Technology (A099) diluted in block/diluent buffer was added and incubated for 2 h at room temperature. After aspirating and washing as above, 50 μl/well of chicken IgY anti-human C1q (custom production by Ayes Labs, Inc using Sigma human C1q, C0660) diluted 8,000-fold in block/diluent buffer, was added. After incubation for 1.5 h at room temperature the wells were aspirated and washed as above. Donkey F(ab′) 2 anti-chicken IgY HRP conjugate (Jackson ImmunoResearch 703-030-155) diluted to 5,000-fold in block/diluent was then added at 50 μl/well and incubated for 1 h at room temperature. After aspirating and washing as above, 100 μl TMB substrate (420 μM TMB, 0.004% H2O2 in 0.1 M sodium acetate/citric acid buffer, pH 4.9) was added and incubated for 2 min before stopping with 100 μl 2 N sulfuric acid. The absorbance was read at 450 nm with a Softmax PRO instrument and Softmax software was used to determine the relative binding affinity (C value) with a 4-parameter fit.
FIG. 21 shows that while BIIB059 is capable of binding C1q, 24F4A IgG4.P/IgG1 agly is essentially devoid of C1q binding.
Example 30. Cell Depletion Studies
BIIB059 potently inhibits type I IFN and IL-6 production after BDCA2 ligation. In addition to its agonistic activity, these experiments were conducted to evaluate whether BIIB059 could deplete BDCA2 bearing pDCs by virtue of its functional Fc. To investigate the cytotoxic potency of BIIB059 its activity in ADCC and CDC assays was tested.
a) ADCC Assay
ADCC is a mechanism whereby an effector cell of the immune system actively lyses a target cell, whose surface receptors have been bound by antibodies (FIG. 22).
The CHO cell line (EAG2456 T1F2 Clone 34.16.7) was used as the target cell. Expression level of BDCA2 on the surface of CHO cells was determined by FACS using an APC-labeled anti-BDCA2 mAb (clone AC144, Miltenyi). NK cells were used as the effector cells and were separated from whole blood by negative selection using the RosetteSepTM Human NK Cell Enrichment Cocktail (Stem Cells Technologies). After a 20 minute incubation with the cocktail at room temperature, NK cells were isolated by discontinuous gradient centrifugation over ficoll. CHO cells and human NK cells were seeded at a ratio of 5:1 (NK:CHO) in the presence of effector competent anti-BDCA2 mAbs (24F4S and BIIB059), Fc crippled mAbs (24F4S-Agly and 24F4A-Agly) or IgG1 isotype control and incubated for 4 hours at 37° C. The negative control consisted of wells containing CHO and NK cells without antibodies. NK and CHO cells lysed with Tx-100 were used to determine maximal killing. ADCC was evaluated using the Vybrant Cytotoxicity Assay kit (Invitrogen), following the manufacturer's instructions. The assay detects G6PD from damaged cells based on the G6PD-dependent reduction of resazurin which emits fluorescence at 590 nm after excitation at 530 nm. The ADCC assay depicted in FIG. 22 panel A was performed using high BDCA2 expressing CHO cells (panel C) while the ADCC assay in FIG. 22 panel B used CHO cells with lower BDCA2 expression (panel D).
24F4S led to 100% killing of BDCA2 bearing CHO cells similar to triton X lysing. As expected the aglycosylated version of the mAb (24F4S-agly) did not lead to ADCC (FIG. 22A). When compared with 24F4S, BIIB059 had an identical ADCC activity (FIG. 22 B). Of note, the killing efficiency correlated with the level of BDCA2 expression on CHO cells (FIG. 22C and FIG. 22D).
b) CDC Assay
In complement-dependent cytotoxicity (CDC), C1q binds the antibody triggering the complement cascade and leading to cell lysis (FIG. 23). As shown in section Example 29, BIIB059 can efficiently bind complement component C1q. This experiment was performed to confirm that BIIB059 can mediate CDC.
CHO-BDCA2/FcεRIγ stable transfectant cells (EAG2456 T1F2 Clone 34.16.7) were seeded at 5×104 cells in 96 well Collagen black well plates and incubated at 37° C. for 48 hrs. The plates were then washed and incubated with rabbit serum complement and propidium iodide (PI) in the presence of effector competent anti-BDCA2 mAbs (24F4S and BIIB059), effector function deficient mAbs (24F4S-Agly and 24F4A-Agly) or IgG1 isotype control for 1 h at 37° C. Negative control consisted of wells containing CHO cells, rabbit serum complement, and PI, without antibodies. NK and CHO cells lysed with T-100× were used to determine maximal killing. The plates were read using Cytoflour Fluorescence plate reader (ex530/em645). Anti-BDCA2 mAbs (BIIB059 and 24F4S) led to cell killing by CDC similar to Triton lysis. As expected effector-deficient aglycosylated mAbs (24F4S-Agly and 24F4A-Agly) did not mediate CDC (FIG. 23). BIIB059 has the potential to deplete BDCA2 bearing pDCs by virtue of its functional IgG1 Fc region. While BIIB059 is capable of cytotoxic activity in BDCA2 over-expressing cells it is not expected to deplete in vivo owing to the rapid, sustained and near-complete internalization of the receptor after BIIB059 ligation.
Example 31. Cloning of a Rat BDCA2 Homolog and Screening for Binding by BIIB059
When the human BDCA2 cDNA sequence is BLASTed against rat sequences in the NCBI database, the closest homolog is rat Clec4b2, described in GenBank® accession number NM_001005896. To determine whether the lead hu24F4 H4/L1 C95A mAb was capable of binding to a rat homolog of human BDCA2, cDNAs were cloned and constructed expression vectors for rat Clec4b2 and rat FcεRIγ. The full-length rat Clec4b2 protein sequence shares only 51.0% identity with human BDCA2. The gapped alignment of human BDCA2 (upper) and rat Clec4b2 (lower) is shown below:




Rat Clec4b2 was cloned by RT-PCR from rat spleen first strand cDNA with primers 5′ GAC CTT CTG AAT ATA TGC GGC CGC CAT GAT GCA GGA AAA AC 3′ (SEQ ID NO: 83) (which adds a 5′ NotI site and Kozak sequence immediately before the Clec4b2 initiator methionine) and 5′ CCC ACA GCC ATG GAG GAC AGG ATC CTC ATA AGT ATA TTT TC 3′ (SEQ ID NO: 84) (which adds a 3′ BamHI site immediately after the Clec4b2 terminator). The 0.64 kb RT-PCR product was purified and subcloned into Invitrogen's pCR2.1TOPO cloning vector, producing the construct pCN815, whose insert was sequenced. Site-directed mutagenesis was performed on template pCN815 with primers 5′ CAG GAT TTC ATC AAC GGA ATC CTA GAC ACT CGT TGG G 3′ (SEQ ID NO:85) and its reverse complement, to correct a PCR error, resulting in the construct pCN822, whose Clec4b2 deduced protein sequence was confirmed to be identical to that in NM_001005896. A mammalian expression vector for rat Clec4b2 full-length cDNA was constructed by ligating the 0.64 kb NotI-BamHI fragment from pCN822 with the 1.89 kb BamHI-XbaI and 4.17 kb XbaI-NotI vector backbone fragments from the expression vector pV90, to produce the expression vector pCN834, whose cDNA insert sequence was confirmed.
The rat FcεRIγ cDNA is described in GenBank® accession number NM_001131001. The rat FcεRIγ protein sequence shares 90.7% identity with human FcεRIγ: the alignment, with human (upper) and rat (lower) is shown below:




Rat FcεRIγ cDNA was cloned by RT-PCR from rat spleen first strand cDNA with primers 5′ CCC AGC GCT GCA GCC CGC GGC CGC CAT GAT CCC AGC GGT 3′ (SEQ ID NO: 87) (which adds a Nod site and Kozak sequence immediately before the FcεRIγ initiator methionine) and 5′ GAA CAC GTG TTG GGA TCC TAT TGG GGT GGT TTC TC 3′ (SEQ ID NO:88) (which adds a 3′ BamHI site immediately after the FcεRIγ terminator). The 0.27 kb RT-PCR product was purified and subcloned into Invitrogen's pCR2.1TOPO cloning vector, producing the construct pCN816, whose insert was sequenced and confirmed to be identical to that in NM_001131001. The 0.27 kb NotI-BamHI fragment from pCN816 was ligated to the 0.66 kb BamHI-XhoI and 4.16 kb XhoI-NotI vector backbone fragments from pBHS103, to construct the mammalian expression vector pCN844, whose rat FcεRIγ cDNA insert sequence was confirmed.
To determine whether the lead hu24F4 H4/L1 C95A mAb was capable of binding to surface rat Clec4b2, 293E cells were transiently co-transfected with an EGFP reporter expression vector (pEAG1458) and either human BDCA2/FcεRIγ vectors (pEAG2420 and pEAG2413) or rat Clec4b2/FcεRIγ vectors (pCN834 and pCN844) at 1:1:1 molar ratios. At 3 days post-transfection cells were harvested and stained with the lead hu24F4 H4/L1 C95A mAb in a dilution titration direct FACS binding assay, gating on live EGFP-positive cells. Although high affinity binding by hu24F4 to surface human BDCA2 was observed, no binding to surface rat Clec4b2 was detected. This indicates that hu24F4 has no cross-reactivity to the closest rat homolog of human BDCA2.
Example 32. Administration of BIIB059 to Healthy Cynomolgus Monkeys Results in Loss of BDCA2 from the Plasmacytoid Dendritic Cell Surface, Likely Via Internalization
In order to assess whether BDCA2 surface levels changed upon administration of BIIB059 to cynomolgus monkeys, two assays were used. The first assay, the so-called “direct” method, detects surface bound BIIB059 with an anti-human PE-labeled secondary antibody. Ideally, a non-cross blocking antibody to BDCA2 would be used to detect total BDCA2; however, such an antibody does not exist. Thus, in the second assay, the so-called “indirect” method, unoccupied BDCA2 is detected through the addition of BIIB059 conjugated to A647.
Prior to administration of any test articles, for each cynomolgus monkey, the maximal mean fluorescence intensity (MFI) for BIIB059 binding to the pDCs was established at 3 different time points (weeks −3, −2, and −1 prior to single injection of BIIB059). At each time point, titration of unlabeled BIIB059 (40 to 0.04 μg/mL final concentration) was added to aliquots of blood, and BIIB059 was detected using a PE-labeled secondary antibody (“direct” method), or free BDCA2 evaluated with BIIB059-A647 (“indirect” method). The maximal values were taken from the values at the plateau in each assay (FIGS. 24 and 25). Evaluation of the values revealed very modest fluctuation in the maximal MFI for each cynomolgus monkey, with more variation between cynomolgus monkeys, showing that the BDCA2 density on pDCs in cynomolgus monkey is variable (Table 2).







TABLE 2







Summary of average EC50 binding of BIIB059 to cell surface BDCA2 on


pDCs in cynomolgus monkey whole blood


Whole blood was drawn from twelve cynomolgus monkeys, once a week


for three weeks total. Blood was incubated with various concentrations


of BIIB059 human IgG1 (0.04 to 40 ug/mL, 6-point curve, 1:4 fold


dilutions). pDCs were identified using flow cytometry as


CD20−CD14−CD123+HLA-DR+, and treated with anti-


human IgG PE labeled secondary to detect BIIB059 bound to the


BDCA2 receptor on pDCs. MFI of PE was calculated with


FlowJo software, and EC50 curves were generated in


GraphPad Prism software.










Cynomolgus
EC50



monkey Donor
(ug/mL)














1
0.81



2
1



3
0.95



4
1.7



5
0.71



6
1.3



7
1.1



8
1.2



9
1.4



10 
1.2



11 
1.4



12 
1.6



average
1.2



SD
0.3







* average of 2-3 experiments






After administration of the vehicle, no BIIB059 was found as expected, and no significant change in BDCA2 levels was found as assessed by binding of BIIB059-A647 (10 μg/ml) (FIG. 26).
After intravenous (IV) administration of BIIB059 at either 10 mg/kg or 1 mg/kg, no BIIB059 was detected on the surface, even as early as 1 hour post injection of BIIB059 (FIGS. 27 and 28). Also, there was no free BDCA2 as assessed by lack of BIIB059-A647 through 38 days for all treated cynomolgus monkeys, with the exception of cynomolgus monkey 5; the serum concentrations in this cynomolgus monkey dropped rapidly on Day 10, likely due to immunogenicity developed against BIIB059.
After subcutaneous administration of a lower dose of BIIB059 (0.2 mg/kg), BIIB059 was briefly observed on the surface of pDCs (at 1 hour, disappeared by 6 hours). At the same time point (1 hr), some free BDCA2 was observed (13%, 74%, 72% of baseline MFI). Again, no drug was detected throughout rest of the study, and no free BDCA2 receptor was detected until day 14 post BIIB059 injection (FIG. 29).
In all cynomolgus monkeys, the reappearance of free BDCA2 coincided with a drop in serum drug levels below 1 μg/ml (FIGS. 30 and 31). Thus, 1 μg/ml appears to be the minimal concentration of BIIB059 needed to mediate internalization of all surface BDCA2.
Table 3 summarizes the EC10, EC50, and EC90 internalization of the BDCA2 receptor on pDCs upon ligation with BIIB059 in cynomolgus monkey whole blood. EC10-50-90 curves were generated in GraphPad Prism software using a four-parameter fit.















Cynomolgus

Dose
EC10
EC50
EC90


monkey
Route
(mg/kg)
(ug/mL)
(ug/mL)
(ug/mL)




















5
IV
1
0.003
0.087
0.370


6
IV
1
0.022
0.025
0.055


7
IV
1
0.014
0.090
0.580


3
IV
10
0.100
0.150
0.220


8
IV
10
0.095
0.370
1.455


10
IV
10
0.114
0.126
0.265


4
SC
0.2
0.078
0.088
0.100


6
SC
0.2
0.040
0.046
0.054


12
SC
0.2
0.114
0.121
0.129


Mean


0.064
0.123
0.359


Stdev


0.045
0.101
0.445









To summarize, the experiments described in this example show that: in vivo IV administration of high doses (10, and 1 mg/kg) of BIIB059 led to rapid disappearance of both available BDCA2 and bound drug from the cell surface, suggesting receptor internalization. Subcutaneous administration of a low dose (0.2 mg/kg) of BIIB059 resulted in a very transient (at 1 hr) detection of BIIB059 on the pDC surface. By 6 hours, no BIIB059 was detectable on the pDC cell surface. Reappearance of available BDCA2 on cell surface occurred when drug exposure declined below 1 μg/mL.
Example 33. BIIB059 Inhibits Pro-Inflammatory Mediators in Addition to all Types of Type I IFN
BDCA2 ligation suppresses the ability of pDCs to produce type I IFNs in response to TLR ligands (see FIG. 16). To confirm the inhibitory activity of the anti-BDCA2 mAb, BIIB059, purified pDCs from healthy human donors were stimulated with the synthetic TLR9 ligand, CpG-A, in the presence of 10 μg/mL BIIB059 or isotype control mAbs. Specifically, pDCs from human healthy donors were isolated using a two-step magnetic bead separation procedure (MACS kit, Miltenyi Biotec). 5×104 purified human pDCs/well were left untreated (Media) or were stimulated with 1 μM TLR9 ligand (CPG-A) in the presence of either 10 μg/mL of BIIB059 (CpG-A+BIIB059) or isotype control (CpG-A+Iso). The plates containing pDCs were incubated for 18 hours at 37° C. and supernatants collected for use in ELISA or multiplex assays to measure concentrations of inflammatory cytokines and chemokines. These experiments showed that BIIB059 potently inhibited TLR9-induced IFNα and other pDC-derived cytokines such as TNFα and IL-6 as well as TLR-9 induced chemokines such as CCL3, CCL4, CCL5 (FIG. 32).
The ability of BIIB059 to inhibit the production of IFNα and pro-inflammatory mediators following stimulation with a physiologically relevant ligand, immune complexes, was also investigated. Specifically, Sm/RNP immune complexes (IC) were pre-formed by mixing sm-RNP from calf thymus and anti-RNP antibodies purified form sera of SLE patients for 1 h in serum-free medium. pDCs from human healthy donors were isolated using a two-step magnetic bead separation procedure (MACS kit, Miltenyi Biotec). 5×104 pDCs/well were left untreated (Media) or were stimulated with pre-formed Sm/RNP IC in the presence of either 10 μg/mL of BIIB059 (IC+BIIB059) or isotype control (IC+Iso). The plates containing pDCs were incubated for 18 hours at 37° C. and supernatants collected for use in ELISA or multiplex assays to measure concentrations of inflammatory cytokines and chemokines. These studies showed that BIIB059 potently inhibited Sm/RNP immune complexes-induced IFNα and other pDC-derived cytokines such as TNFα and IL-6. BIIB059 also inhibited chemokines induced by Sm/RNP immune complexes, such as CCL3 and CCL4 (FIG. 33).
Example 34: BIIB059 Inhibits Sm/RNP IC-Induced Transcription of Type I IFN Subtypes by Purified Human pDCs
Thirteen IFNα subtypes and a single member of IFNβ exist in humans. The effect of BIIB059 on the transcription of type I IFN subtypes in Sm/RNP IC stimulated pDCs from healthy human donors was evaluated using qualitative polymerase chain reaction (qPCR) assays.
Sm/RNP immune complexes (IC) were pre-formed by mixing sm-RNP from calf thymus and anti-RNP antibodies purified from sera of SLE patients for 30 minutes in serum-free medium. pDCs from human healthy donors were isolated using a two-step magnetic bead separation procedure (MACS kit, Miltenyi Biotec). 7.5×105 purified human pDCs/well were left untreated (Media) or were stimulated with pre-formed Sm/RNP IC in the presence of either 10 μg/mL of BIIB059 (IC+BIIB059) or isotype control (IC+Iso). The plates containing pDC were incubated for 16 hours at 37° C. and 5% CO2. pDC cells were collected and RNA from pDC was isolated for evaluation in qPCR reaction.
This experiment showed that treatment with BIIB059 inhibited the transcript level of all type I IFN subtypes tested (FIG. 34).
Example 35: BIIB059 Inhibits TLR9-Induced IFNα Production by Human PBMC from Healthy Donors and SLE Patients
pDCs are the major producers of IFN in response to TLR7 and TLR9 stimulation. pDCs can produce thousand-fold more IFN than any other cell type. This experiment investigates whether BIIB059 could inhibit TLR9-induced IFNα production in peripheral blood mononuclear cell (PBMC) cultures without the need for pDC isolation. PBMC from healthy human donors or SLE patients were stimulated with 1 or 5 μM of the TLR9 ligand (CpG-A) and treated with concentrations of BIIB059 ranging from 10 μg/mL to 2 ng/mL in a total assay volume of 250 μL/well. The plates were incubated overnight (18 hours) at 37° C. and 5% CO2. Supernatants were collected for evaluation in IFNα ELISA assays.
This experiment showed that BIIB059 inhibited TLR9-induced IFNα production by PBMC from healthy donors with an average IC50 of 0.04+/−0.05 μg/mL (FIGS. 35A and 35C). BIIB059 showed similar potency at inhibiting TLR9-induced IFNα production by PBMC from SLE patients with an average IC50 of 0.03+/−0.01 μg/mL (FIGS. 35B and 35C).
Example 36: BIIB059 Inhibits IFNα Production in Whole Blood Stimulated with TLR9 Ligand
The activity of BIIB059 was also evaluated in whole blood assays (WBA). Whole blood from healthy human donors was stimulated with TLR9 ligand in the presence of increasing concentrations of BIIB059 and the IC50 of inhibition was calculated for each individual donor. Specifically, whole blood from healthy human donors was incubated with increasing concentrations of BIIB059 ranging from 10 μg/mL to 2 ng/mL or isotype control in a total assay volume of 200 μl/well. CpG-A was added at 75 μg/mL (open square), which was determined to be optimal for stimulation of IFNα production in whole blood. Plates were incubated for 18 hours at 37° C. and supernatants collected for use in IFNα ELISA assays (PBL InterferonSource).
BIIB059 showed a dose dependent inhibition of TLR9-induced IFNα production in whole blood assays and exhibited similar IC50 to that seen with PBMC cultures (FIG. 36).
Example 37: BIIB059 does not Inhibit TLR3-Induced IFNα Production by Human PBMC from Healthy Human Donors
This experiment was performed to determine whether other cell types triggered with different TLR ligands are still able to produce type I IFN even in the presence of BIIB059. TLR3 is not expressed in pDCs and therefore TLR3 ligand does not induce IFN production by pDCs. PBMC from human healthy donors were stimulated with poly:IC, which is a TLR3 ligand that can potently induce type I IFN predominantly by monocytes. Specifically, PBMC from healthy human donors were stimulated with 1 μM of the TLR3 ligand (Poly I: C) and treated with concentrations of BIIB059 ranging from 10 μg/mL to 0.5 ng/mL in a total assay volume of 250 μL/well in a 96 well plate. The plates were incubated overnight (18 hours) at 37° C. and 5% CO2. 200 μL of the supernatants were collected for evaluation of IFNα levels by ELISA. As shown in FIG. 37, BIIB059 did not impact TLR3-induced IFNα production by PBMC from healthy human donors.
To summarize, Examples 33-37 show that BIIB059 can potently inhibit TLR 9-stimulated type I interferon by purified pDCs, PBMC, and whole blood cultures. BIIB059 is equally potent at inhibiting TLR9-induced Type I interferon by pDCs from healthy human donors and SLE patients. In addition to inhibiting type I IFN, BIIB059 can inhibit the production of other pDC-derived cytokines and chemokines. BIIB059 specifically inhibits TLR9-induced type I IFN by pDCs and does not impact IFN production by other cell types triggered with a different TLR ligand. Therefore, the in vitro data provided herein support the pharmacological activity and potency of BIIB059 in addition to its specificity for TLR7/9-induced type I IFN by pDCs.
Example 38: BIIB059 Mediates BDCA2 Internalization on Human pDCs
To determine whether BIIB059 induces BDCA2 internalization, human whole blood from 10 healthy human donors was incubated with increasing concentrations of BIIB059 at 37° C. for 16 hours. The remaining cell surface BDCA2 was detected using a FITC-labeled non-cross blocking anti-BDCA2 mAb (clone 2D6).
Specifically, whole blood from 10 healthy human donors was incubated with increasing concentrations of BIIB059 or 10 μg/ml isotype control antibody for 16 hours at 37° C. and 5% CO2 and then incubated for 30 minutes at 4° C. with FITC-labeled non-cross blocking anti-BDCA2 mAb (clone 2D6), anti-HLA-DR, anti-CD123, anti-CD14 and anti-CD20. Whole blood was then incubated for 30 minutes at 4° C. with 50 μL of a staining solution, which included the following mAbs: FITC-labeled non-cross blocking anti-BDCA2 mAb (clone 2D6), anti-HLA-DR, anti-CD123, anti-CD14 and anti-CD20. Red blood cells (RBCs) were lysed using 1× lyse/fix buffer (BD Bioscience).
As shown in FIG. 38, BIIB059 led to a dose dependent decrease in the intensity of FITC-labeled 2D6 staining with an average EC50 of 0.017±0.005 μg/mL
Example 39: BDCA2 is Rapidly Internalized Upon Ligation with BIIB059
To determine the kinetics of BIIB059-induced BDCA2 internalization, human whole blood was incubated with different concentrations of BIIB059 at 37° C. for various periods. Specifically, whole blood was treated with 10, 1, 0.1 or 0.01 μg/mL of BIIB059 or an isotype control antibody (10 μg/ml) at 37° C. for the periods indicated. The whole blood was then incubated for 30 minutes at 4° C. with 50 μL of a staining solution which included the following mAbs: FITC-labeled non-cross blocking anti-BDCA2 mAb (clone 2D6), anti-HLA-DR, anti-CD123, anti-CD14 and anti-CD20. Red blood cells (RBCs) were lysed and fixed using 1× Lyse/fix buffer (BD Bioscience). As shown in FIG. 39, upon incubation with BIIB059 at 1 μg/ml the intensity of FITC-labeled 2D6 staining rapidly decreased reaching background levels within one hour of incubation. Incubation with a tenfold lower BIIB059 concentration (0.1 μg/ml) delayed internalization of BDCA2 by 2 hours. This data shows that the rate of BDCA2 internalization is dependent on the dose of BIIB059.
Example 40: BIIB059 Induce BDCA2 Internalization in Human Plasmacytoid Dendritic Cells
To visualize the internalization of BDCA2 after ligation with BIIB059, purified pDCs were incubated with A647-labeled BIIB059 and analyzed by confocal microscopy. As expected, BDCA2 was localized on the cell surface of pDCs at 4° C. After a short incubation at 37° C. BDCA2 was clearly detected inside the cells (FIG. 40).
Example 41: Internalization does not Alter BIIB059-Mediated Inhibition of IFN-α Production
This experiment investigated whether BDCA2 internalization alters the ability of BIIB059 to inhibit TLR9-induced IFNα production by pDCs. Cells were pre-incubated with BIIB059 at 37° C. for various periods corresponding to maximal BDCA2 internalization and then stimulated with TLR9 ligand for an additional 18 hours. Specifically, whole blood was collected from heparinized venous blood of healthy donors and pre-incubated with BIIB059 or isotope control antibody for the duration indicated. At each time point after pre-incubation, cells were stimulated with 200 μg/mL TLR9 ligand (CpG-A) and incubated for an additional 18 hours at 37° C. Supernatants were collected for use in IFNα ELISA assays (PBL InterferonSource). As shown in FIG. 41, pre-incubation with BIIB059 (up to 9 hours) did not alter the ability of BIIB059 to inhibit TLR9-induced IFNα production in whole blood assays from healthy human donors. These data suggest that BDCA2 internalization might be required for the inhibition of TLR9 signaling.
Example 42: The EC50 of BIIB059-Mediated BDCA2 Internalization on pDCs Correlates with the IC50 of BIIB059-Mediated Inhibition of TLR9-Induced IFNα by pDCs in Whole Blood Assays
To further explore the link between the internalization of BDCA2 and the inhibition of TLR9 signaling, the potency of BIIB059-mediated internalization of BDCA2 on pDCs and the inhibition of TLR-mediated IFNα production by pDCs was compared in 10 healthy human donors.
To evaluate BIIB059-mediated BDCA2 internalization, whole blood was incubated with BIIB059 for 16 hours. The whole blood was then collected, lysed, and BDCA2 expression was assessed by flow cytometry using the FITC-conjugated non-cross blocking antibody 2D6. To evaluate BIIB059-mediated inhibition of TLR9 induced IFNα by pDCs, whole blood was incubated with increasing concentrations of BIIB059 for 16 hours in the presence of a TLR9 ligand. The supernatants were harvested and evaluated for IFNα by ELISA. The EC50 of BIIB059-mediated BDCA2 internalization was 0.02 μg/mL. The IC50 of BIIB059 mediated inhibition of TLR9 induced IFNα was 0.07 μg/mL. A correlation between the EC50 of BIIB059-mediated internalization of BDCA2 and the IC50 of BIIB059 IFNα inhibition was observed with an R square value of 0.57 (FIG. 42).
Example 43: TLR9 Activation Induces BDCA2 Colocalization with TLR9 and with the Lysosomal Marker LAMP1
To test the hypothesis that BIIB059-mediated TLR9 inhibition requires internalization and localization of BDCA2 into endosomal/lysosomal compartments containing TLR9, confocal microscopy was used to follow the intracellular distribution of BDCA2 after BIIB059 ligation. Purified human pDCs were cultured for 7 days and incubated with A647-labeled BIIB059 for 15 min at 37° C. During the last 10 min of the incubation, cells were treated with 1 μM of the TLR9 ligand CpG-A or left untreated. Cells were stained with fluorescently labeled antibodies to TLR9 and the late endosomal/lysosomal marker, LAMP1, and analyzed by confocal microscopy.
TLR9 was recruited to a late endosomal/lysosomal compartment after stimulation with TLR9 ligand, as evidenced by increased colocalization of TLR9 with LAMP1 (FIG. 43). TLR9 stimulation also significantly increased the fraction of BDCA2 colocalizing with TLR9 and LAMP1. These results suggest that BIIB059, when bound to BDCA2, preferentially localizes to intracellular compartments where activated TLR9 is present.
In sum, Examples 38-43 show that BIIB059, a humanized monoclonal antibody against BDCA2, engages BDCA2 and leads to its internalization. Upon stimulation, BDCA2 colocalize with TLR9 in the endosomal/lysosomal compartment where it mediates inhibition of TLR9 signaling. These data suggest that BDCA2 internalization is a necessary step for mediating the inhibition of TLR9-induced pro-inflammatory mediators by pDCs.
Example 44: Effect of BIIB059 on CD62L Levels
Circulating pDCs express high levels of CD62L (L-selectin) and home to high endothelial venules (HEV)-containing lymphoid tissue. PNAd is a ligand for CD62L that is constitutively expressed on HEV and mediates homing of CD62L expressing cells to organized lymphoid tissue. PNAd was found to be expressed by dermal endothelial cells in cutaneous Lupus Erythematosis lesions. By virtue of their CD62L expression pDCs could be recruited to inflamed peripheral tissues expressing PNAd.
To determine whether BIIB059 impacts the expression of CD62L on the surface of human pDCs, whole blood was treated with varying concentrations of BIIB059 for 1 hour at 37° C. without stimulation. Specifically, whole blood from healthy human donors was treated with increasing concentrations of BIIB059 for 1 hour at 37° C. and 5% CO2. The MFI of CD62L was determined by gating on pDCs as defined by CD14−, CD20−, HLA-DR+ and CD123+.
BIIB059 caused a dose-dependent decrease in CD62L expression on the surface of human pDCs as assessed by flow cytometry (FIG. 44). Stimulation of pDCs with TLR ligand did not impact the expression of CD62L (FIG. 44A).
Example 45: Treatment of PBMC with GM6001 Inhibits BIIB059 Mediated CD62L Shedding from the Surface of Human pDCs
Metalloproteinase are known to induce CD62L shedding from the surface of immune cells. To investigate whether metalloproteinases are involved in the BIIB059-mediated decrease of surface CD62L, PBMC were prepared from healthy human donors and pre-treated with GM6001 (a metalloproteinase inhibitor) for 30 minutes at 37° C. and 5% CO2, followed by the addition of 10 μg/mL of BIIB059 for 1 hour. The surface expression of CD62L was assayed by flow cytometry. GM6001 inhibited the BIIB059-mediated down-modulation CD62L in a dose dependent manner (FIG. 45). These data suggest that BIIB059 induces CD62L shedding in a metalloproteinase-dependent manner.
In sum, Examples 44 and 45 show that BIIB059 decreases the expression of CD62L on the surface of human pDCs. BIIB059-mediated CD62L downmodulation is inhibited by metalloproteinase inhibitor (GM6001) indicating that BIIB059 induces CD62L shedding from the surface of human pDCs through, at least in part, the activation of metalloproteinases. BIIB059 treatment is therefore expected to reduce or prevent trafficking of pDCs to target organs in SLE.
Example 46: Impact of the Fc Region of BIIB059 on Immune-Complex-Mediated IFN Production by Plasmacytoid Dendritic Cells
Fc gamma receptor IIA (CD32a) is a cell surface protein that binds IgG with low affinity. Human plasmacytoid dendritic cells exclusively express Fc gamma receptor IIA, CD32a. Stimulation of pDCs with immune complexes has been shown to be dependent on CD32 Immune complexes are internalized by CD32 and stimulate endosomal TLR7/9 to induce IFN production by pDCs.
To determine the effect of BIIB059 on CD32a surface expression, isolated pDCs were treated with increasing concentrations of BIIB059 or the aglycosylated form of the antibody, 24F4-A, and incubated for 16-hour at 37° C. pDCs were then stained with FITC-labeled BDCA2 and PE-labeled anti-CD32 (clone AT10) and the surface expression of BDCA2 and CD32 was assessed by flow cytometry. BIIB059 and the agly version, 24F4-A, were equally potent in their ability to induce BDCA2 internalization (FIG. 46A). Only BIIB059 was able to induce the down-modulation of CD32 on the cell surface of pDCs as indicated by the dose-dependent decrease in CD32 Mean Fluorescent Intensity (MFI) (FIGS. 46 B-D). Treatment with effector competent istoype control had no effect on CD32 surface levels (FIG. 46). These data indicate that the BIIB059-mediated down-modulation of CD32a levels on the surface of pDCs is specific to the binding of the Fc region of BIIB059.
To ensure that binding of the Fc region of BIIB059 does not merely mask the epitope of CD32 recognized by the FITC-labeled the anti-CD32 mAb, pDCs were treated with 10 μg/mL of BIIB059 for 1 hour at 4° C. or 37° C. and then stained with labeled anti-CD32. As shown in FIG. 46E, treatment with BIIB059 at 4° C. did not decrease the CD32 MFI indicating that treatment with BIIB059 does not interfere with the binding of labeled anti-CD32 mAb. The fact that CD32a down-modulation occurred only upon incubation with BIIB059 at 37° C. suggests that CD32a could be lost from the cell surface of pDCs.
To determine whether the down-modulation of CD32a by BIIB059 has a biological impact, pDCs were incubated in the presence of increasing concentrations of BIIB059 or the aglycosylated form, 24F4A-Agly, and stimulated with either immune complexes or the synthetic TLR9 ligand (CPG-A). As expected, BIIB059 and 24F4A-Agly were undistinguishable in their ability to inhibit CPG-A-induced IFNα by pDCs, which is CD32 independent (FIG. 47A). There was a clear separation in potency between BIIB059 and 24F4A-agly when the pDCs were stimulated with immune complexes. BIIB059 inhibited immune complex-induced IFNα with an IC50 of 0.04 compared to an IC50 of 1.4 μg/mL by 24F4A-Agly. (FIG. 47B). These data indicate that BIIB059 down-modulates CD32a by virtue of its functional Fc and therefore inhibits stimulation of pDCs by immune complexes.
To confirm that the down-modulation of CD32a was unique to BIIB059, we investigated the effect of a fully humanized anti-CD40 antibody on CD32 levels and immune-complex mediated IFNα production by pDCs. CD40 is a cell surface protein expressed on pDCs. An anti-CD40 antibody with a fully functional Fc has the ability to engage CD40 and bind CD32 on the surface of pDCs. Treatment with anti-CD40 mAb had no effect of CD32 surface expression and no significant effect on IFNα production from immune complex stimulated pDCs (FIGS. 48A and B). Binding of anti CD40 mAb was confirmed by demonstrating maximal CD40 engagement in anti-CD40 treated cells (FIG. 48C).
As shown previously, BDCA2 ligation with BIIB059 or the aglycosylated form 24F4A-Agly leads to receptor internalization and inhibition of TLR9-induced IFNα by pDCs. In this study we show that BIIB059 causes down-modulation of CD32a on pDCs and inhibition of immune complex-stimulated IFNα production by pDCs in an Fc dependent manner. The CD32a down-modulation triggered by BIIB059 does not result from just any antibody with a functional Fc that can bind a cell surface molecule expressed on pDCs. This study highlights the novel therapeutic potential of an effector competent anti-BDCA2 mAb, which can dampen pDC responses through both its Fab′2 and Fc regions leading to enhanced efficacy.
Example 47: Interaction of BIIB059 with Hydroxychloroquine (HCQ)
Antimalarial agents, such as hydroxychloroquine (HCQ), have been used in the treatment of SLE. pDCs from SLE patients treated with HCQ have impaired ability to produce IFNα upon stimulation with TLR7 and TLR9 ligands. Since both BIIB059 and HCQ impact TLR7/9 induced IFNα in pDCs, it was investigated whether the effect of BIIB059 and HCQ could be redundant.
To address this question, human PBMC were prepared from blood from healthy donors and stimulated with either TLR7 or TLR9 ligands in the presence of varying concentrations of BIIB059 alone, HCQ alone, or BIIB059 in combination with HCQ. Supernatants were harvested after 18 hours and assayed for IFNα by ELISA. The addition of HCQ increased the potency of BIIB059 and led to an additive inhibitory effect on TLR7 and TLR9-induced IFNα production by PBMC from healthy human donors. These data demonstrate that the activity of BIIB059 and HCQ are not redundant and highlight the additional therapeutic benefit of BIIB059 when administered with antimalarial compounds such as HCQ.
Example 48: Effect of BIIB059 on BDCA2-Expressing pDCs In Vivo
The objective of this study was to determine if administration of BIIB059 to cynomolgus monkey mediates depletion of pDCs in the peripheral blood.
Four pre-BIIB059 dosing bleeds were collected at weekly intervals from twelve cynomolgus monkeys to establish a baseline pDC frequency for each animal (Table 3).







TABLE 3







Summary of average circulating pDC frequencies in healthy 


cynomolgus monkey whole blood.


Whole blood was drawn from twelve cynomolgus monkeys 


once a week for four weeks total. pDCs were identified using flow 


cytometry as CD20−CD14−CD123+HLA-DR+. pDC as a percent 


of CD20−CD14− cells was calculated with FlowJo software.








Cynomolgus



monkey
percent circulating pDC













donor
073112
080712
081312
082013
average
SD
















1
0.26
0.2
0.15
0.16
0.19
0.05


2
0.2
0.15
0.15
0.21
0.18
0.03


3
0.11
0.06
0.11
0.19
0.12
0.05


4
0.12
0.11
0.14
0.31
0.17
0.09


5
0.19
0.2
0.31
0.40
0.28
0.10


6
0.32
0.57
0.35
0.39
0.41
0.11


7
0.15
0.19
0.21
0.16
0.18
0.03


8
0.12
0.13
0.1
0.16
0.13
0.03


9
0.08
0.12
0.1
0.11
0.10
0.02


10
0.16
0.15
0.28
0.22
0.20
0.06


11
0.06
0.07
0.04
0.07
0.06
0.01


12
0.1
0.05
0.07
0.16
0.10
0.05


average
0.16
0.17
0.17
0.21
0.18



SD
0.08
0.14
0.10
0.10
0.09









In all statistical analyses, pDC frequencies were log-transformed to reduce the skewness (FIG. 51). The original distribution of pDC frequencies in the left panel of FIG. 51 was severely right-skewed. However, after a log-transformation, the distribution of the transformed pDC frequencies (FIG. 51, right panel) approximately followed a normal distribution. These log-transformed data were used for all statistical analysis methods. FIG. 52 shows levels of pDC on log scale for each cynomolgus monkey over four time points prior to IV injection. Using a linear mixed effects model with four time points as fixed factors and random intercepts for cynos, we concluded that the geometric means of pDC percentages for all monkeys were equal over the 4 predose time points (FIG. 53, p-value for time based on a F-test: 0.67). This analysis indicated that the geometric mean of percentage of circulating pDCs was relatively stable over time for cynomolgus monkeys.
Nine of these twelve cynomolgus monkeys were divided into 3 groups (3/group), and randomized to include equal representation of BDCA2 density and percent pDC in each group. Cynomolgus monkeys received a single intravenous injection of either vehicle (sodium citrate), 10 mg/kg BIIB059, or 1 mg/kg BIIB059. Flow cytometry was used to identify circulating pDCs in the whole blood as CD20−CD14−CD123+HLA-DR+, and the pDC frequency (on log-scale) at each time point was graphed in R software (FIG. 54). A linear mixed effects model was fitted to log (pDC) frequencies using random intercepts for cynomolgus monkeys and fixed effects for dose group and time period: 1 hour, 6 hours, 1-27 days, and greater than 28 days. To assess whether pDC changed among different dose groups at different time periods, a preliminary model also included the interaction terms for dose group and different time periods. The p-value based on F-test for testing all interaction terms equal to 0 is 0.81, which indicates that there is no difference for the pDC changes among different dose groups. Hence, the final fitted model only included the statistically significant effects for time period and dose group factors (Table 4).







TABLE 4







Fitted model estimates for time points after a single intravenous


BIIB059 or vehicle injection.


Estimates for the fixed effects using a linear mixed effects model using


random intercepts for cynomolgus monkeys, and fixed factors for dose


group and time levels 1 hour, 6 hours, and greater than 28 days,


for percent circulating pDC on log scale before and after


IV dose of sodium citrate vehicle, BIIB059 1 mg/kg,


or BIIB059 at 10 mg/kg in cynomolgus monkeys.













exp






(effect est.)





effect est.
(% pDC ratio)
95% CI
P














time: 1 hr v.
−0.56
0.57
0.43 to 0.77
0.0003


others


time: 6 hrs v.
0.46
1.58
1.18 to 2.13
0.003


others


time: >28 days v.
−0.48
0.62
0.55 to 0.70
<0.0001


others


Group: BIIB059
0.49
1.64
1.20 to 2.25
0.01


1 mg/kg v. vehicle


Group: BIIB059
0.09
1.09
0.79 to 1.50
0.84


10 mg/kg v. vehicle









The parameter estimates for the fixed factors were exponentiated in order to interpret them as the ratios of pDC frequencies at these time periods compared to pre-BIIB059 dosing. Overall, the ratio was significantly less than one when comparing the pDC frequencies at 1-hour after IV injection to pre dose pDC frequencies (95% CI: 0.43-0.77, p-value: 0.0003). The ratio was significantly greater than one comparing the pDC frequencies at 6 hours after IV injection to the predose pDC frequencies (95% CI: 1.18-2.12, p-value: 0.003). The ratio was not significantly different from one when comparing the pDC frequencies 1-28 day period after IV injection to the predose pDC frequencies. The ratio was significantly less than one when comparing the pDC frequencies after 28 days after IV injection to the predose pDC frequencies (95% CI: 0.55-0.70, p-value: <0.0001). The final fitted model was plotted in FIG. 55. The results revealed that there was a significant in vivo depletion of circulating pDCs in cynomolgus monkeys at 1 hour, a significant increase of circulating pDCs at 6 hours and a significant depletion of circulating pDCs after 28 days after IV injection, but changes in percent pDC across time were the same for all treatment groups.
Additionally, after the completion of the IV study time points, three of these cynomolgus monkeys (4, 6, and 12) received a single subcutaneous dose of BIIB059 at 0.2 mg/kg, to evaluate the effect of a lower dose on circulating pDC frequencies. The pDC frequency (on log-scale) at each time point was graphed in R software (FIG. 56). A linear mixed effects model was fitted, using continuous time and time at 1 hour as fixed factors, and cynomolgus monkeys as random intercepts. The results are shown in Table 5.







TABLE 5







Fitted model estimates for time points after a single subcutaneous


BIIB059 injection.


Estimates for the fixed effects using a linear mixed effects model using


continuous time and time at 1 hour as fixed factors, and cynomolgus


monkeys as random intercepts for percent circulating pDC on log scale,


before and after a single subcutaneous injection of


BIIB059 0.2 mg/kg in cynomolgus monkeys













exp (effect est.)





effect est.
(% pDC ratio)
95% CI
P














time (continuous)
0.01
1.01
1.00 to 1.02
<0.0001


time: 1 hr v. others
−0.78
0.46
0.34 to 0.65
<0.0001









Similar to the previous results, we observed a significant in vivo depletion of circulating pDCs in cynomolgus monkeys at 1 hour after IV injections (95% CI: 0.34-0.55, p-value<0.0001), but the geometric mean of % pDC for the three cynomolgus monkeys increased steadily as time increased (95% CI: 1.00-1.03 fold change per day, p-value<0.0001). The fitted model was plotted in FIG. 57.
In conclusion, these data show that BIIB059 does not mediate a sustained depletion of pDCs in the blood of cynomolgus monkeys when administered at the tested doses. This is likely due to internalization of BDCA2.
Example 49: Administration of BIIB059 to Cynomolgus Monkeys Results in Inhibition of TLR9-Induced IFNα Production in Ex Vivo Whole Blood Assay
The objective of this study was to determine whether BIIB059, when administered to cynomolgus monkeys in vivo, could alter the production of IFNα in response to TLR9 stimulation in an ex vivo whole blood assay (WBA).
Intravenous and subcutaneous dosing routes were evaluated for their ability to impact IFNα induction, which was measured using the MxA bioassay according to the experimental plan outlined in FIG. 58. TLR9 ligand (CpG-A) induced measurable quantities of IFNα in whole blood cultures across all time points and in all cynomolgus monkeys, while no IFNα was detected in the control PBS-treated cultures (data not shown).
For the intravenously-dosed cynomolgus monkeys, IFNα values post-treatment were calculated as percentages of the pre-dose mean for each animal. Data for bleeds after day 14 were excluded from the analysis as the whole blood assay was not performed for the 10 mg/kg BIIB059 group after this time point. A trend towards reduced % IFNα relative to pre-dose mean was observed on several days following drug administration in the 1 mg/kg and 10 mg/kg BIIB059 dosing groups compared to the vehicle group (FIG. 59)
A more comprehensive analysis of the data was performed using two-way mixed effects analysis of variance (ANOVA) to estimate the mean IFNα and the post versus pre differences for each dose group in the IV-study. Data during the first 24 hours following dosing were excluded due to an observed decrease in peripheral blood plasmacytoid dendritic cell percentages. Data for bleeds after day 31 post-dose were excluded from the analysis due to the return of BDCA2 expression observed at this time. For the vehicle dosed group, the geometric mean IFNα was 362 Units/mL (U/mL) pre-dose, and 314 U/mL post-dose; for the 1 mg/kg dosed group, the geometric mean was 399 U/mL pre-dose, and 237 U/mL post-dose; for the 10 mg/kg group, the geometric mean IFNα was 211 U/mL pre-dose, and 102 U/mL post-dose (FIG. 4). The post-pre differences in mean log 10 IFNα were −0.061 (p=0.511) for the Vehicle group, −0.226 (p=0.016) for the 1 mg/kg group, and −0.317 (p=0.004) for the 10 mg/kg group. After anti-log 10 transformation, these results revealed that the Vehicle group had 10^(−0.061)=87% (95% CI: 57%-133%) of the IFNα concentration post-dose compared to pre-dose; the 1 mg/kg group had 10^(−0.226)=59% (95% CI: 39%-91%) of the IFN concentration post-dose compared to pre-dose; and the 10 mg/kg group had 10^(−0.317)=48% (95% CI: 29%-79%) of the IFN concentration post-dose compared to pre-dose (FIG. 60).
For the subcutaneously-dosed cynomolgus monkey cohort, a one-way analysis of variance (ANOVA) with random effects was used to estimate the mean IFNα and the post versus pre differences for the entire group. Data during the first 24 hours following dosing were excluded due to an observed decrease in peripheral blood plasmacytoid dendritic cell percentages. Data for bleeds after day 33 post-dose were excluded from the analysis due to the recovery of BDCA2 expression observed at this time. For the subcutaneously dosed group, the geometric mean IFNα was 1243 U/mL pre-dose and 812 U/mL post-dose, yielding a post/pre ratio of 65%. The post-pre difference in mean log 10 was estimated to be −0.185 (p=0.059) which, after anti-log 10 transformation, corresponds to 10^(−0.185)=65% of the pre-dose geometric mean; the 95% CI of this effect is 41%-102% (FIG. 61).
As only a small number of cynomolgus monkeys were used in the experiment, the IFNα concentration determined for each monkey highly influences the results for that group. The proportion of variation due to animal differences in the intravenous study was 69% of the total variability, with the remainder being primarily due to differences between time points within cynomolgus monkey (26%), and a small amount (<6%) due to assay sources of variation. The variation between cynomolgus monkeys is much larger than the variation between time points within cynomolgus monkeys, suggesting that adding cynomolgus monkeys to this experiment as opposed to more bleeding time points would better power the study. The proportion of variation due to cynomolgus monkey differences in the subcutaneous study was 45% of the total variability, with the remainder being mostly due to differences between time points within cynomolgus monkey, and a negligible amount (<2%) due to assay sources of variation.
The variability observed across cynomolgus monkeys and within cynomolgus monkeys may be due to a number of factors, including fluctuations in physiological conditions of the cynomolgus monkeys, cellular composition of the blood, molecular composition of the cell, and precision of the functional assay.
While there was some fluctuation in plasmacytoid dendritic cell percentages in each animal over time, the % of pDCs in the blood was not affected by treatment with BIIB059 (See Rsch-2013-046) and did not show consistent correlation with IFNα production. Additionally, a rapid and sustained loss of BDCA2 from the cell surface was observed on pDCs following IV and SC BIIB059 administration, suggesting high level of receptor occupancy (See Rsch-2013-043). Taking into account the high level of variability in the responsiveness of pDCs from cynomolgus monkeys to TLR9 stimulation, there was a trend towards dampened IFNα responses following intravenous and subcutaneous administration of BIIB059, with the greatest reduction in the 10 mg/kg IV-dosed group, followed by the 0.2 mg/kg SC-group and then the 1 mg/kg IV-group.
In conclusion, BIIB059 when dosed in vivo to cynomolgus monkeys, showed a trend towards inhibited TLR9-induced IFN production in an ex vivo WBA.
OTHER EMBODIMENTS
While the invention has been described in conjunction with the detailed description thereof, the foregoing description is intended to illustrate and not limit the scope of the invention, which is defined by the scope of the appended claims. Other aspects, advantages, and modifications are within the scope of the following claims.

## Claims

1. An antibody that specifically binds to human Blood dendritic cell antigen 2 (BDCA2) consisting of the amino acid sequence set forth in SEQ ID NO: 1, wherein the antibody comprises a variable heavy (VH) domain comprising VH complementarity determining region (CDR)1, VH CDR2, and VH CDR3, wherein:
the VH CDR1 comprises the amino acid sequence GFTFSTYTMS (SEQ ID NO:9);
the VH CDR2 comprises the amino add sequence TISPGDSFGYYYPDSVQG (SEQ ID NO: 10); and
the VH CDR3 comprises the amino add sequence DIYYNYGAWFAY (SEQ ID NO: 11); and
wherein the antibody comprises a variable light (VL) domain comprising VL CDR1, VL CDR2, and VL CDR3, wherein:
the VL CDR1 comprises the amino add sequence KASQSVDYDGDSYMN (SEQ ID NO:5);
the VL CDR2 comprises the amino add sequence AASTLES (SEQ ID NO:6); and
the VL CDR3 comprises the amino add sequence QQANEDPRT (SEQ ID NO:7).

2. The antibody of claim 1, wherein the VH domain comprises the amino add sequence set forth in SEQ ID NO:24.

3. The antibody of claim 1, wherein the antibody comprises a heavy chain and wherein the heavy chain comprises the amino add sequence set forth in SEQ ID NO:4.

4. The antibody of claim 1, wherein the VL domain comprises the amino add sequence set forth in SEQ ID NO:23.

5. The antibody of claim 1, wherein the antibody comprises a light chain and wherein the light chain comprises the amino acid sequence set forth in SEQ ID NO:3.

6. The antibody of claim 1, wherein the VH domain comprises the amino acid sequence set forth in SEQ ID NO:24 and the VL domain comprises the amino acid sequence set forth in SEQ ID NO:23.

7. The antibody of claim 1, wherein the antibody comprises a heavy chain and a light chain, and wherein the heavy chain comprises the amino acid sequence set forth in SEQ ID NO:4 and the light chain comprises the amino acid sequence set forth in SEQ ID NO:3.

8. The antibody of claim 1, wherein the antibody is a humanized antibody.

9. The antibody of claim 1, wherein the antibody is a chimeric antibody.

10. The antibody of claim 1, wherein the antibody comprises an IgG1 heavy chain constant region.

11. An antigen-binding fragment that specifically binds to human Blood dendritic cell antigen 2 (BDCA2) consisting of the amino acid sequence set forth in SEQ ID NO:1, wherein the antigen-binding fragment comprises a variable heavy (VH) domain comprising VH complementarity determining region (CDR)1, VH CDR2, and VH CDR3, wherein:
the VH CDR1 comprises the amino add sequence GFTFSTYTMS (SEQ ID NO:9);
the VH CDR2 comprises the amino add sequence TISPGDSFGYYYPDSVQG (SEQ ID NO: 10); and
the VH CDR3 comprises the amino add sequence DIYYNYGAWFAY (SEQ ID NO: 11); and
wherein the antigen-binding fragment comprises a variable light (VL) domain comprising VL CDR1, VL CDR2, and VL CDR3, wherein:
the VL CDR1 comprises the amino add sequence KASQSVDYDGDSYMN (SEQ ID NO:5);
the VL CDR2 comprises the amino add sequence AASTLES (SEQ ID NO:6); and
the VL CDR3 comprises the amino add sequence QQANEDPRT (SEQ ID NO:7).

12. The antigen-binding fragment of claim 11, wherein the VH domain comprises the amino add sequence set forth in SEQ ID NO:24.

13. The antigen-binding fragment of claim 11, wherein the VL domain comprises the amino add sequence set forth in SEQ ID NO:23.

14. The antigen-binding fragment of claim 11, wherein the VH domain comprises the amino add sequence set forth in SEQ ID NO:24 and the VL domain comprises the amino add sequence set forth in SEQ ID NO:23.

15. The antigen-binding fragment of claim 11, wherein the antigen-binding fragment is: a single chain antibody, an Fab fragment, an F(ab′)2 fragment, an Fab′ fragment, an Fsc fragment, an Fv fragment, an scFv, an sc(Fv)2, or a diabody.

16. An isolated cell that produces the antibody of claim 1.

17. An isolated cell that produces the antigen-binding fragment thereof of claim 11.

18. A pharmaceutical composition comprising the antibody of claim 1 and a pharmaceutically acceptable carrier.

19. A pharmaceutical composition comprising the antibody of claim 6 and a pharmaceutically acceptable carrier.

20. A pharmaceutical composition comprising the antibody of claim 7 and a pharmaceutically acceptable carrier.

21. A pharmaceutical composition comprising the antigen-binding fragment of claim 11 and a pharmaceutically acceptable carrier.

22. The pharmaceutical composition of claim 18, wherein the composition comprises 10 to 25 mM citrate, 100 to 200 mM sodium chloride, and a pH of 5.5 to 6.5.

23. The pharmaceutical composition of claim 22, wherein the composition comprises 20 mM sodium citrate, 150 mM sodium chloride, and a pH of 6.0.

24. A method of reducing production of a type I interferon, IL-6, TNF-α, CCL3, CCL4, IP10, and RANTES by a plasmacytoid dendritic cell expressing BDAC2 in a human subject, the method comprising contacting a plasmacytoid dendritic cell that expresses BDCA2 with an effective amount of the antibody of claim 1.

25. A method of treating an inflammatory disorder associated with the presence of plasmacytoid dendritic cells expressing BDCA2 in a human subject having said inflammatory disorder, comprising administering to the human subject an effective amount of the antibody of claim 1.

26. The method of claim 25, wherein the inflammatory disorder is selected from the group consisting of systemic lupus erythematosus, discoid lupus, lupus nephritis, rheumatoid arthritis, inflammatory bowel disease, systemic sclerosis (scleroderma), psoriasis, type I diabetes, dermatomyositis, and polymyositis.

27. The method of claim 25, wherein the inflammatory disorder is systemic lupus erythematosus, discoid lupus, lupus nephritis, or cutaneous lupus.

28. The method of claim 25, wherein the inflammatory disorder is systemic lupus erythematosus.

29. The method of claim 25, wherein the inflammatory disorder is cutaneous lupus erythematosus.

30. The method of claim 25, wherein the inflammatory disorder is moderate to severe lupus with active central nervous system (CNS) and/or renal involvement.

31. The method of claim 25, wherein the inflammatory disorder is moderate to serve lupus without active central nervous system (CNS) and/or renal involvement.

32. A method of treating an autoimmune disease associated with the presence of plasmacytoid dendritic cells expressing BDCA2 in a human subject having said autoimmune disease, comprising administering to the human subject an effective amount of the antibody of claim 1.

33. A method of reducing production of a type 1 interferon, IL-6, TNF-α, CCL3, CCL4, IP10, and RANTES by a plasmacytoid dendritic cell expressing BDCA2 in a human subject, the method comprising contacting a plasmacytoid dendritic cell that expresses BDCA2 with an effective amount of the antibody of claim 6.

34. A method of treating an inflammatory disorder associated with the presence of plasmacytoid dendritic cells expressing BDCA2 in a human subject having said inflammatory disorder, comprising administering to the human subject an effective amount of the antibody of claim 6.

35. The method of claim 34, wherein the inflammatory disorder is selected from the group consisting of systemic lupus erythematosus, discoid lupus, lupus nephritis, rheumatoid arthritis, inflammatory bowel disease, systemic sclerosis (scleroderma), psoriasis, type I diabetes, dermatomyositis, and polymyositis.

36. The method of claim 34, wherein the inflammatory disorder is systemic lupus erythematosus, discoid lupus, lupus nephritis, or cutaneous lupus.

37. The method of claim 34, wherein the inflammatory disorder is moderate to severe lupus with active central nervous system (CNS) and/or renal involvement.

38. The method of claim 34, wherein the inflammatory disorder is moderate to severe lupus without active central nervous system (CNS) and/or renal involvement.

39. The method of claim 34, wherein the inflammatory disorder is systemic lupus erythematosus.

40. The method of claim 34, wherein the inflammatory disorder is cutaneous lupus erythematosus.

41. A method of treating an autoimmune disease associated with the presence of plasmacytoid dendritic cells expressing BDCA2 in a human subject having said autoimmune disease, comprising administering to the human subject an effective amount of the antibody of claim 6.

42. A method of inducing death of a plasmacytoid dendritic cell expressing BDCA2 in a human subject, the method comprising contacting a plasmacytoid dendritic cell expressing BDCA2 of the human subject with the antibody of claim 6, wherein the contacting induces death of the plasmacytoid dendritic cell.

43. A method of reducing production of a type I interferon, IL-6, TNF-α, CCL3, CCL4, IP10, and RANTES by a plasmacytoid dendritic cell expressing BDCA2 in a human subject, the method comprising contacting a plasmacytoid dendritic cell that expresses BDCA2 with an effective amount of the antibody of claim 7.

44. A method of treating an inflammatory disorder associated with the presence of plasmacytoid dendritic cells expressing BDCA2 in a human subject having said inflammatory disorder, comprising administering to the human subject an effective amount of the antibody of claim 7.

45. The method of claim 44, wherein the inflammatory disorder is selected from the group consisting of systemic lupus erythematosus, discoid lupus, lupus nephritis, rheumatoid arthritis, inflammatory bowel disease, systemic sclerosis (scleroderma), psoriasis, type 1 diabetes, dermatomyositis, and polymyositis.

46. The method of claim 44, wherein the inflammatory disorder is systemic lupus erythematosus, discoid lupus, lupus nephritis, or cutaneous lupus.

47. The method of claim 44, wherein the inflammatory disorder is moderate to severe lupus with active central nervous system (CNS) and/or renal involvement.

48. The method of claim 44, wherein the inflammatory disorder is moderate to severe lupus without active central nervous system (CNS) and/or renal involvement.

49. The method of claim 44, wherein the inflammatory disorder is systemic lupus erythematosus.

50. The method of claim 44, wherein the inflammatory disorder is cutaneous lupus erythematosus.

51. A method of treating an autoimmune disease associated with the presence of plasmacytoid dendritic cells expressing BDCA2 in a human subject having said autoimmune disease, comprising administering to the human subject an effective amount of the antibody of claim 7.

52. A method of inducing death of a plasmacytoid dendritic cell expressing BDCA2 in a human subject, the method comprising contacting a plasmacytoid dendritic cell expressing BDCA2 of the human subject with the antibody of claim 7, wherein the contacting induces death of the plasmacytoid dendritic cell.

53. A method of inducing death of a plasmacytoid dendritic cell expressing BDCA2 in a human subject, the method comprising contacting a plasmacytoid dendritic cell expressing BDCA2 of the human subject with the antibody of claim 1, wherein the contacting induces death of the plasmacytoid dendritic cell.