# Patent

## Title
Quinoxaline derivatives useful as FGFR kinase modulators

## Abstract

The invention relates to new quinoxaline derivative compounds of formula (I), to pharmaceutical compositions comprising said compounds, to processes for the preparation of said compounds and to the use of said compounds in the treatment of diseases, e.g. cancer.

## Background
CROSS REFERENCE TO RELATED APPLICATIONS
This application is a national stage filing under section 371 of International Application No. PCT/EP2015/056507, filed on Mar. 26, 2015, and published on Oct. 1, 2015 as WO 2015/144803, and claims priority to European Application No. 14161820.7, filed on Mar. 26, 2014. The entire disclosures of each of the prior applications are hereby incorporated herein by reference.
FIELD OF THE INVENTION
The invention relates to new quinoxaline derivative compounds, to pharmaceutical compositions comprising said compounds, to processes for the preparation of said compounds and to the use of said compounds in the treatment of diseases, e.g. cancer.
SUMMARY OF THE INVENTION
According to a first aspect of the invention there is provided compounds of formula (I):




including any tautomeric or stereochemically isomeric form thereof, wherein
n represents an integer equal to 1 or 2;
R1 represents hydrogen, C1-6alkyl, hydroxyC1-6alkyl, C1-6alkyl substituted with —C(═O)NHCH3, or C1-6alkyl substituted with —S(═O)2— C1-4alkyl;
R2a represents hydrogen, fluoro or chloro;
R2b or R2c each independently represent methoxy or hydroxyl;
R3 represent hydrogen, C1-6alkyl, C3-6cycloalkyl, or C1-2alkyl substituted with C3-6cycloalkyl;
R4 represents hydrogen, methyl or ethyl;
the pharmaceutically acceptable salts thereof or the solvates thereof.
In one embodiment there is provided compounds of formula (Ia):




including any tautomeric or stereochemically isomeric form thereof, wherein
n represents an integer equal to 1 or 2;
R1 represents hydrogen, C1-6alkyl, hydroxyC1-6alkyl, C1-6alkyl substituted with —C(═O)NHCH3, or C1-6alkyl substituted with —S(═O)2— C1-4alkyl;
R2a represents hydrogen, fluoro or chloro;
R2b or R2c each independently represent methoxy or hydroxyl;
R3 represent hydrogen, C1-6alkyl, C3-6cycloalkyl, or C1-2alkyl substituted with C3-6cycloalkyl; the pharmaceutically acceptable salts thereof or the solvates thereof.
In one embodiment there is provided compounds of formula (Ib):




including any tautomeric or stereochemically isomeric form thereof, wherein n represents an integer equal to 1 or 2;
R1 represents hydrogen, C1-6alkyl, hydroxyC1-6alkyl, C1-6alkyl substituted with —C(═O)NHCH3, or C1-6alkyl substituted with —S(═O)2— C1-4alkyl;
R2b or R2c each independently represent methoxy or hydroxyl;
R3 represent hydrogen, C1-6alkyl, C3-6cycloalkyl, or C1-2alkyl substituted with C3-6cycloalkyl;
the pharmaceutically acceptable salts thereof or the solvates thereof.
In one embodiment there is provided compounds of formula (Ic):




including any tautomeric or stereochemically isomeric form thereof, wherein
R1 represents hydrogen, C1-6alkyl, hydroxyC1-6alkyl, C1-6alkyl substituted with —C(═O)NHCH3, or C1-6alkyl substituted with —S(═O)2— C1-4alkyl;
R2b or R2c each independently represent methoxy or hydroxyl;
the pharmaceutically acceptable salts thereof or the solvates thereof.
In one embodiment there is provided compounds of formula (Id):




including any tautomeric or stereochemically isomeric form thereof, wherein
R2b or R2c each independently represent methoxy or hydroxyl;
the pharmaceutically acceptable salts thereof or the solvates thereof.
In one embodiment there is provided compounds of formula (Ie):




including any tautomeric or stereochemically isomeric form thereof, wherein
R1 represents hydrogen, C1-6alkyl, hydroxyC1-6alkyl, C1-6alkyl substituted with —C(═O)NHCH3, or C1-6alkyl substituted with —S(═O)2— C1-4alkyl;
R2b or R2c each independently represent methoxy or hydroxyl;
the pharmaceutically acceptable salts thereof or the solvates thereof.
WO2006/092430, WO2008/003702, WO01/68047, WO2005/007099, WO2004/098494, WO2009/141386, WO2004/030635, WO2008/141065, WO2011/026579, WO2011/028947, WO2007/003419, WO00/42026, WO2012/154760, WO2011/047129, WO2003/076416, WO2002/096873, WO2000/055153, EP548934, U.S. Pat. No. 4,166,117, WO2011/135376, WO2012/073017, WO2013/061074, WO2013/061081, WO2013/061077, WO2013/061080, WO2013/179034, WO2013/179033, WO2014/174307 which each disclose a series of heterocyclyl derivatives.


DETAILED DESCRIPTION OF THE INVENTION
Unless the context indicates otherwise, references to formula (I) in all sections of this document (including the uses, methods and other aspects of the invention) include references to all other sub-formula (e.g. Ia, Ib, Ic, Id), sub-groups, preferences, embodiments and examples as defined herein.
The prefix “Cx-y” (where x and y are integers) as used herein refers to the number of carbon atoms in a given group. Thus, a C1-6alkyl group contains from 1 to 6 carbon atoms, a C3-6cycloalkyl group contains from 3 to 6 carbon atoms, a hydroxyC1-6alkyl group contains from 1 to 6 carbon atoms, and so on.
The term ‘C1-2alkyl’, ‘C1-4alkyl’, or ‘C1-6alkyl’ as used herein as a group or part of a group refers to a linear or branched saturated hydrocarbon group containing 1 or 2, or from 1 to 4 or 1 to 6 carbon atoms. Examples of such groups include methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, n-pentyl, isopentyl, neopentyl or hexyl and the like.
The term ‘C3-6cycloalkyl’ as used herein refers to a saturated monocyclic hydrocarbon ring of 3 to 6 carbon atoms. Examples of such groups include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl.
The term ‘hydroxyC1-4alkyl’ or ‘hydroxyC1-6alkyl’ as used herein as a group or part of a group refers to a C1-4alkyl or C1-6alkyl group as defined herein wherein one or more than one hydrogen atom is replaced with a hydroxyl group. The terms ‘hydroxyC1-4alkyl’ or ‘hydroxyC1-6alkyl’ therefore include monohydroxyC1-4alkyl, monohydroxyC1-6alkyl and also polyhydroxyC1-4alkyl and polyhydroxyC1-6alkyl. There may be one, two, three or more hydrogen atoms replaced with a hydroxyl group, so the hydroxyC1-4alkyl or hydroxyC1-6alkyl may have one, two, three or more hydroxyl groups. Examples of such groups include hydroxymethyl, hydroxyethyl, hydroxypropyl and the like.
In one embodiment, in a compound of formula (I), n represents an integer equal to 1.
In one embodiment, in a compound of formula (I), n represents an integer equal to 2.
In one embodiment, in a compound of formula (I), R1 represents hydrogen or C1-6alkyl, in particular C1-6alkyl, more in particular methyl.
In one embodiment, in a compound of formula (I), R1 represents hydrogen or C1-6alkyl, in particular C1-6alkyl, more in particular ethyl.
In one embodiment, in a compound of formula (I), R1 represents hydrogen.
In one embodiment, in a compound of formula (I), R2a represents hydrogen or fluoro.
In one embodiment, in a compound of formula (I), R2a represents hydrogen.
In one embodiment, in a compound of formula (I), R2a represents fluoro.
In one embodiment, in a compound of formula (I), R2b represents methoxy.
In one embodiment, in a compound of formula (I), R2b represents hydroxy.
In one embodiment, in a compound of formula (I), R2c represents methoxy.
In one embodiment, in a compound of formula (I), R2c represents hydroxy.
In one embodiment, in a compound of formula (I), R2b represents methoxy and R2c represents hydroxyl.
In one embodiment, in a compound of formula (I), R2b represents hydroxyl and R2c represents methoxy.
In one embodiment, in a compound of formula (I), R2b and R2c both represent methoxy.
In one embodiment, in a compound of formula (I), R2b and R2c both represent hydroxyl.
In one embodiment, in a compound of formula (I), R3 represents hydrogen.
In one embodiment, in a compound of formula (I), R3 represents C1-6alkyl, in particular C1-4alkyl, even more in particular isopropyl.
In one embodiment, in a compound of formula (I), R3 represents C1-6alkyl, in particular C1-4alkyl, even more in particular methyl.
In one embodiment, in a compound of formula (I), R4 represents hydrogen.
In one embodiment, in a compound of formula (I), R4 represents methyl or ethyl.
In one embodiment, in a compound of formula (I),
n represents an integer equal to 1;
R1 represents C1-6alkyl, in particular C1-4alkyl, more in particular methyl;
R2a represents hydrogen or fluoro, in particular hydrogen;
R2b represents methoxy;
R2c represents methoxy;
R3 represents hydrogen or C1-6alkyl, in particular C1-6alkyl, more in particular C1-4alkyl, even more in particular isopropyl;
R4 represents hydrogen.
In one embodiment, in a compound of formula (I),
n represents an integer equal to 1 or 2;
R1 represents C1-6alkyl, in particular C1-4alkyl, more in particular methyl or ethyl;
R2a represents hydrogen or fluoro, in particular hydrogen;
R2b represents methoxy;
R2 represents methoxy;
R3 represents hydrogen or C1-6alkyl, in particular C1-6alkyl, more in particular C1-4alkyl, even more in particular isopropyl or methyl;
R4 represents hydrogen.
In one embodiment, in a compound of formula (Ia), n represents an integer equal to 1.
In one embodiment, in a compound of formula (Ia), n represents an integer equal to 2.
In one embodiment, in a compound of formula (Ia), R1 represents hydrogen or C1-6alkyl, in particular C1-6alkyl, more in particular methyl.
In one embodiment, in a compound of formula (Ia), R1 represents hydrogen or C1-6alkyl, in particular C1-6alkyl, more in particular ethyl.
In one embodiment, in a compound of formula (Ia), R1 represents hydrogen.
In one embodiment, in a compound of formula (Ia), R2a represents hydrogen or fluoro.
In one embodiment, in a compound of formula (Ia), R2a represents hydrogen.
In one embodiment, in a compound of formula (Ia), R2a represents fluoro.
In one embodiment, in a compound of formula (Ia), R2b represents methoxy.
In one embodiment, in a compound of formula (Ia), R2b represents hydroxy.
In one embodiment, in a compound of formula (Ia), R2c represents methoxy.
In one embodiment, in a compound of formula (Ia), R2c represents hydroxy.
In one embodiment, in a compound of formula (Ia), R2b represents methoxy and R2c represents hydroxyl.
In one embodiment, in a compound of formula (Ia), R2b represents hydroxyl and R2c represents methoxy.
In one embodiment, in a compound of formula (Ia), R2b and R2c both represent methoxy.
In one embodiment, in a compound of formula (Ia), R2b and R2c both represent hydroxyl.
In one embodiment, in a compound of formula (Ia), R3 represents hydrogen.
In one embodiment, in a compound of formula (Ia), R3 represents C1-6alkyl, in particular C1-4alkyl, even more in particular isopropyl.
In one embodiment, in a compound of formula (Ia), R3 represents C1-6alkyl, in particular C1-4alkyl, even more in particular methyl.
In one embodiment, in a compound of formula (Ia),
n represents an integer equal to 1;
R1 represents C1-6alkyl, in particular C1-4alkyl, more in particular methyl;
R2a represents hydrogen or fluoro, in particular hydrogen;
R2b represents methoxy;
R2c represents methoxy;
R3 represents hydrogen or C1-6alkyl, in particular C1-6alkyl, more in particular C1-4alkyl, even more in particular isopropyl.
In one embodiment, in a compound of formula (Ia),
n represents an integer equal to 1 or 2;
R1 represents C1-6alkyl, in particular C1-4alkyl, more in particular methyl or ethyl;
R2a represents hydrogen or fluoro, in particular hydrogen;
R2b represents methoxy;
R2c represents methoxy;
R3 represents hydrogen or C1-6alkyl, in particular C1-6alkyl, more in particular C1-4alkyl, even more in particular isopropyl or methyl.
In one embodiment, in a compound of formula (Ib), n represents an integer equal to 1.
In one embodiment, in a compound of formula (Ib), n represents an integer equal to 2.
In one embodiment, in a compound of formula (Ib), R1 represents hydrogen or C1-6alkyl, in particular C1-6alkyl, more in particular methyl.
In one embodiment, in a compound of formula (Ib), R1 represents hydrogen or C1-6alkyl, in particular C1-6alkyl, more in particular ethyl.
In one embodiment, in a compound of formula (Ib), R1 represents hydrogen.
In one embodiment, in a compound of formula (Ib), R2b represents methoxy.
In one embodiment, in a compound of formula (Ib), R2b represents hydroxy.
In one embodiment, in a compound of formula (Ib), R2c represents methoxy.
In one embodiment, in a compound of formula (Ib), R2c represents hydroxy.
In one embodiment, in a compound of formula (Ib), R2b represents methoxy and R2c represents hydroxyl.
In one embodiment, in a compound of formula (Ib), R2b represents hydroxyl and R2c represents methoxy.
In one embodiment, in a compound of formula (Ib), R2b and R2c both represent methoxy.
In one embodiment, in a compound of formula (Ib), R2b and R2c both represent hydroxyl.
In one embodiment, in a compound of formula (Ib), R3 represents hydrogen.
In one embodiment, in a compound of formula (Ib), R3 represents C1-6alkyl, in particular C1-4alkyl, even more in particular isopropyl.
In one embodiment, in a compound of formula (Ib), R3 represents C1-6alkyl, in particular C1-4alkyl, even more in particular methyl.
In one embodiment, in a compound of formula (Ib),
n represents an integer equal to 1;
R1 represents C1-6alkyl, in particular C1-4alkyl, more in particular methyl;
R2b represents methoxy;
R2c represents methoxy;
R3 represents hydrogen or C1-6alkyl, in particular C1-6alkyl, more in particular C1-4alkyl, even more in particular isopropyl.
In one embodiment, in a compound of formula (Ib),
n represents an integer equal to 1 or 2;
R1 represents C1-6alkyl, in particular C1-4alkyl, more in particular methyl or ethyl;
R2b represents methoxy;
R2c represents methoxy;
R3 represents hydrogen or C1-6alkyl, in particular C1-6alkyl, more in particular C1-4alkyl, even more in particular isopropyl or methyl.
In one embodiment, in a compound of formula (Ic), R1 represents hydrogen or C1-6alkyl, in particular C1-6alkyl, more in particular methyl.
In one embodiment, in a compound of formula (Ic), R1 represents hydrogen or C1-6alkyl, in particular C1-6alkyl, more in particular ethyl.
In one embodiment, in a compound of formula (Ic), R1 represents hydrogen.
In one embodiment, in a compound of formula (Ic), R2b represents methoxy.
In one embodiment, in a compound of formula (Ic), R2b represents hydroxy.
In one embodiment, in a compound of formula (Ic), R2c represents methoxy.
In one embodiment, in a compound of formula (Ic), R2c represents hydroxy.
In one embodiment, in a compound of formula (Ic), R2b represents methoxy and R2c represents hydroxyl.
In one embodiment, in a compound of formula (Ic), R2b represents hydroxyl and R2c represents methoxy.
In one embodiment, in a compound of formula (Ic), R2b and R2c both represent methoxy.
In one embodiment, in a compound of formula (Ic), R2b and R2c both represent hydroxyl.
In one embodiment, in a compound of formula (Ic),
R1 represents C1-6alkyl, in particular C1-4alkyl, more in particular methyl;
R2b represents methoxy;
R2c represents methoxy.
In one embodiment, in a compound of formula (Ic),
R1 represents C1-6alkyl, in particular C1-4alkyl, more in particular methyl or ethyl;
R2b represents methoxy;
R2c represents methoxy.
In one embodiment, in a compound of formula (Id), R2b represents methoxy.
In one embodiment, in a compound of formula (Id), R2b represents hydroxy.
In one embodiment, in a compound of formula (Id), R2c represents methoxy.
In one embodiment, in a compound of formula (Id), R2c represents hydroxy.
In one embodiment, in a compound of formula (Id), R2b represents methoxy and R2c represents hydroxyl.
In one embodiment, in a compound of formula (Id), R2b represents hydroxyl and R2c represents methoxy.
In one embodiment, in a compound of formula (Id), R2b and R2c both represent methoxy.
In one embodiment, in a compound of formula (Id), R2b and R2c both represent hydroxyl.
In one embodiment, in a compound of formula (Ie), R1 represents hydrogen or C1-6alkyl, in particular C1-6alkyl, more in particular methyl.
In one embodiment, in a compound of formula (Ie), R1 represents hydrogen or C1-6alkyl, in particular C1-6alkyl, more in particular ethyl.
In one embodiment, in a compound of formula (Ie), R1 represents hydrogen.
In one embodiment, in a compound of formula (Ie), R2b represents methoxy.
In one embodiment, in a compound of formula (Ie), R2b represents hydroxy.
In one embodiment, in a compound of formula (Ie), R2c represents methoxy.
In one embodiment, in a compound of formula (Ie), R2c represents hydroxy.
In one embodiment, in a compound of formula (Ie), R2b represents methoxy and R2c represents hydroxyl.
In one embodiment, in a compound of formula (Ie), R2b represents hydroxyl and R2c represents methoxy.
In one embodiment, in a compound of formula (Ie), R2b and R2c both represent methoxy.
In one embodiment, in a compound of formula (Ie), R2b and R2c both represent hydroxyl.
In one embodiment, in a compound of formula (Ie),
R1 represents C1-6alkyl, in particular C1-4alkyl, more in particular methyl;
R2b represents methoxy;
R2c represents methoxy.
In one embodiment, in a compound of formula (Ie),
R1 represents C1-6alkyl, in particular C1-4alkyl, more in particular methyl or ethyl;
R2b represents methoxy;
R2c represents methoxy.
In one embodiment, in a compound of formula (I),
n represents an integer equal to 1 or 2;
R1 represents hydrogen or C1-6alkyl, in particular C1-4alkyl, more in particular methyl or ethyl;
R2a represents hydrogen or fluoro, in particular hydrogen;
R2b represents methoxy or hydroxyl;
R2c represents methoxy or hydroxyl;
R3 represents C1-6alkyl, more in particular C1-4alkyl, even more in particular isopropyl or methyl;
R4 represents hydrogen.
In one embodiment, the compound of formula (I) as defined herein is selected from the following compounds or is one of the following compounds:




a pharmaceutically acceptable salt thereof or a solvate thereof.
In one embodiment, the compound of formula (I) as defined herein is selected from the following compounds or is one of the following compounds:







a pharmaceutically acceptable salt thereof or a solvate thereof.
For the avoidance of doubt, it is to be understood that each general and specific preference, embodiment and example for one substituent may be combined with each general and specific preference, embodiment and example for one or more, preferably, all other substituents as defined herein and that all such embodiments are embraced by this application.
Methods for the Preparation of Compounds of Formula (I)
In this section, as in all other sections of this application unless the context indicates otherwise, references to formula (I) also include all other sub-groups and examples thereof as defined herein.
In general, compounds of formula (I) can be prepared according to the following reaction scheme




In Scheme 1, the following reaction conditions apply:
1: reaction of an intermediate of formula (II) with formaldehyde in the presence of a suitable solvent, such as for example dioxane, N,N-dimethylformamide, N,N-dimethylacetamide, at a temperature ranging from room temperature to reflux.
It is considered to be within the knowledge of the person skilled in the art to recognize in which condition and on which part of the molecule a protective group may be appropriate. For instance, protective group on the R1 substituent or on the pyrrazole moiety, or protective group on the R3 substituent or on the R2a,b,c substituent or combinations thereof. The skilled person is also considered to be able to recognize the most feasible protective group, such as for example —C(═O)—O—C1-4alkyl or




or O—Si(CH3)2(C(CH3)3) or —CH2—O—CH2CH2—O—CH3.

The present invention also comprises deuterated compounds. These deuterated compounds may be prepared by using the appropriate deuterated intermediates during the synthesis process.
The compounds of formula (I) may also be converted into each other via art-known reactions or functional group transformations.
Compounds of formula (I) wherein R1 represents hydrogen can be converted into a compound of formula (I) wherein R1 represents C1-6alkyl or hydroxyC1-6alkyl, by reaction with C1-6alkyl-W or hydroxyC1-6alkyl-W, wherein W represents a suitable leaving group, such as for example halo, e.g. bromo and the like, in the presence of a suitable base, such as for example sodium hydride or potassium carbonate, and a suitable solvent, such as for example acetonitrile or N,N-dimethylformamide.
Compounds of formula (I) wherein R1 represents hydrogen can also be converted into a compound of formula (I) wherein R1 represents C1-6alkyl-OH, by reaction with W—C1-6alkyl-O—Si(CH3)2(C(CH3)3) in the presence of a suitable base, such as for example sodium hydride, and a suitable solvent, such as for example N,N-dimethylformamide, followed by a deprotection reaction of the silyl protecting group by art-known methods.
Compounds of formula (I) wherein R1 represents hydrogen, can also be converted into a compound of formula (I) wherein R1 represents ethyl substituted with —S(═O)2—C1-6alkyl, by reaction with C1-6alkyl-vinylsulfone, in the presence of a suitable base, such as for example triethylamine, and a suitable solvent, such as for example an alcohol, e.g. methanol or by reaction with C1-6alkyl-2-bromoethylsulfone in the presence of a suitable deprotonating agent, such as for example NaH, and a suitable solvent, such as for example dimethylformamide.
Compounds of formula (I) wherein R2b or R2c represents —OCH3 can be converted into a compound of formula (I) wherein R2b or R2c represents —OH by reaction with boron tribromide in the presence of a suitable solvent, such as for example dichloromethane.
Compounds of formula (I) wherein R2b or R2c represents —OH can be converted into a compound of formula (I) wherein R2b or R2c represents —OCH3 by reaction with methyl iodine in the presence of a suitable base, such as for example potassium carbonate, and a suitable solvent, such as for example N,N-dimethylformamide.
In general, compounds of formula (Id) can also be prepared by incubating them with liver fractions of animals, e.g. rat or human, and subsequently isolating the desired products from the incubation medium according to the following reaction scheme.




Intermediates of formula (II) or (II-a) can be prepared as described in WO2011/135376 (for instance compounds of formula (I-b) or (I-b-3) of WO2011/135376).
A further aspect of the invention is a process for the preparation of a compound of formula (I) as defined herein, which process comprises:
(i) reacting a compound of formula (II) with formaldehyde in the presence of a suitable solvent, such as for example dioxane, N,N-dimethylformamide, N,N-dimethylacetamide, at a suitable temperature, such as a temperature ranging from room temperature to reflux;




    
    
        wherein R1, R2a, R2b, R2c, R3, R4 and n are as defined herein; and optionally thereafter converting one compound of the formula (I) into another compound of the formula (I).
    
    


Pharmaceutically Acceptable Salts, Solvates or Derivatives Thereof
In this section, as in all other sections of this application, unless the context indicates otherwise, references to formula (I) include references to all other sub-groups, preferences, embodiments and examples thereof as defined herein.
Unless otherwise specified, a reference to a particular compound also includes ionic forms, salts, solvates, isomers, tautomers, esters, prodrugs, isotopes and protected forms thereof, for example, as discussed below; preferably, the ionic forms, or salts or tautomers or isomers or solvates thereof; and more preferably, the ionic forms, or salts or tautomers or solvates or protected forms thereof, even more preferably the salts or tautomers or solvates thereof. Many compounds of the formula (I) can exist in the form of salts, for example acid addition salts or, in certain cases salts of organic and inorganic bases such as carboxylate, sulphonate and phosphate salts. All such salts are within the scope of this invention, and references to compounds of the formula (I) include the salt forms of the compounds. It will be appreciated that references to “derivatives” include references to ionic forms, salts, solvates, isomers, tautomers, esters, prodrugs, isotopes and protected forms thereof.
According to one aspect of the invention there is provided a compound as defined herein or a salt, tautomer, or solvate thereof. According to a further aspect of the invention there is provided a compound as defined herein or a salt or solvate thereof. References to compounds of the formula (I) and sub-groups thereof as defined herein include within their scope the salts or solvates or tautomers of the compounds.
The salt forms of the compounds of the invention are typically pharmaceutically acceptable salts, and examples of pharmaceutically acceptable salts are discussed in Berge et al. (1977) “Pharmaceutically Acceptable Salts,” J. Pharm. Sci., Vol. 66, pp. 1-19. However, salts that are not pharmaceutically acceptable may also be prepared as intermediate forms which may then be converted into pharmaceutically acceptable salts. Such non-pharmaceutically acceptable salts forms, which may be useful, for example, in the purification or separation of the compounds of the invention, also form part of the invention.
The salts of the present invention can be synthesized from the parent compound that contains a basic or acidic moiety by conventional chemical methods such as methods described in Pharmaceutical Salts: Properties, Selection, and Use, P. Heinrich Stahl (Editor), Camille G. Wermuth (Editor), ISBN: 3-90639-026-8, Hardcover, 388 pages, August 2002. Generally, such salts can be prepared by reacting the free acid or base forms of these compounds with the appropriate base or acid in water or in an organic solvent, or in a mixture of the two; generally, nonaqueous media such as ether, ethyl acetate, ethanol, isopropanol, or acetonitrile are used. The compounds of the invention may exist as mono- or di-salts depending upon the pKa of the acid from which the salt is formed.
Acid addition salts may be formed with a wide variety of acids, both inorganic and organic. Examples of acid addition salts include salts formed with an acid selected from the group consisting of acetic, 2,2-dichloroacetic, adipic, alginic, ascorbic (e.g. L-ascorbic), L-aspartic, benzenesulphonic, benzoic, 4-acetamidobenzoic, butanoic, (+) camphoric, camphor-sulphonic, (+)-(1S)-camphor-10-sulphonic, capric, caproic, caprylic, cinnamic, citric, cyclamic, dodecylsulphuric, ethane-1,2-disulphonic, ethanesulphonic, 2-hydroxyethanesulphonic, formic, fumaric, galactaric, gentisic, glucoheptonic, D-gluconic, glucuronic (e.g. D-glucuronic), glutamic (e.g. L-glutamic), α-oxoglutaric, glycolic, hippuric, hydrobromic, hydrochloric, hydriodic, isethionic, lactic (e.g. (+)-L-lactic, (±)-DL-lactic), lactobionic, maleic, malic, (−)-L-malic, malonic, (±)-DL-mandelic, methanesulphonic, naphthalenesulphonic (e.g. naphthalene-2-sulphonic), naphthalene-1,5-disulphonic, 1-hydroxy-2-naphthoic, nicotinic, nitric, oleic, orotic, oxalic, palmitic, pamoic, phosphoric, propionic, L-pyroglutamic, pyruvic, salicylic, 4-amino-salicylic, sebacic, stearic, succinic, sulphuric, tannic, (+)-L-tartaric, thiocyanic, toluenesulphonic (e.g. p-toluenesulphonic), undecylenic and valeric acids, as well as acylated amino acids and cation exchange resins.
One particular group of salts consists of salts formed from acetic, hydrochloric, hydriodic, phosphoric, nitric, sulphuric, citric, lactic, succinic, maleic, malic, isethionic, fumaric, benzenesulphonic, toluenesulphonic, methanesulphonic (mesylate), ethanesulphonic, naphthalenesulphonic, valeric, acetic, propanoic, butanoic, malonic, glucuronic and lactobionic acids. Another group of acid addition salts includes salts formed from acetic, adipic, ascorbic, aspartic, citric, DL-Lactic, fumaric, gluconic, glucuronic, hippuric, hydrochloric, glutamic, DL-malic, methanesulphonic, sebacic, stearic, succinic and tartaric acids.
If the compound is anionic, or has a functional group which may be anionic, then a salt may be formed with a suitable cation. Examples of suitable inorganic cations include, but are not limited to, alkali metal ions such as Na+ and K+, alkaline earth metal cations such as Ca2+ and Mg2+, and other cations such as Al3+. Examples of suitable organic cations include, but are not limited to, ammonium ion (i.e., NH4+) and substituted ammonium ions (e.g., NH3R+, NH2R2+, NHR3+, NR4+).
Examples of some suitable substituted ammonium ions are those derived from: ethylamine, diethylamine, dicyclohexylamine, triethylamine, butylamine, ethylenediamine, ethanolamine, diethanolamine, piperazine, benzylamine, phenylbenzylamine, choline, meglumine, and tromethamine, as well as amino acids, such as lysine and arginine. An example of a common quaternary ammonium ion is N(CH3)4+.
Where the compounds of the formula (I) contain an amine function, these may form quaternary ammonium salts, for example by reaction with an alkylating agent according to methods well known to the skilled person. Such quaternary ammonium compounds are within the scope of formula (I). Compounds of the formula (I) containing an amine function may also form N-oxides. A reference herein to a compound of the formula (I) that contains an amine function also includes the N-oxide. Where a compound contains several amine functions, one or more than one nitrogen atom may be oxidised to form an N-oxide. Particular examples of N-oxides are the N-oxides of a tertiary amine or a nitrogen atom of a nitrogen-containing heterocycle. N-Oxides can be formed by treatment of the corresponding amine with an oxidizing agent such as hydrogen peroxide or a per-acid (e.g. a peroxycarboxylic acid), see for example Advanced Organic Chemistry, by Jerry March, 4th Edition, Wiley Interscience, pages. More particularly, N-oxides can be made by the procedure of L. W. Deady (Syn. Comm. (1977), 7, 509-514) in which the amine compound is reacted with m-chloroperoxybenzoic acid (MCPBA), for example, in an inert solvent such as dichloromethane.
The compounds of the invention may form solvates, for example with water (i.e., hydrates) or common organic solvents. As used herein, the term “solvate” means a physical association of the compounds of the present invention with one or more solvent molecules. This physical association involves varying degrees of ionic and covalent bonding, including hydrogen bonding.
In certain instances the solvate will be capable of isolation, for example when one or more solvent molecules are incorporated in the crystal lattice of the crystalline solid. The term “solvate” is intended to encompass both solution-phase and isolatable solvates. Non-limiting examples of suitable solvates include compounds of the invention in combination with water, isopropanol, ethanol, methanol, DMSO, ethyl acetate, acetic acid or ethanolamine and the like. The compounds of the invention may exert their biological effects whilst they are in solution.
Solvates are well known in pharmaceutical chemistry. They can be important to the processes for the preparation of a substance (e.g. in relation to their purification, the storage of the substance (e.g. its stability) and the ease of handling of the substance and are often formed as part of the isolation or purification stages of a chemical synthesis. A person skilled in the art can determine by means of standard and long used techniques whether a hydrate or other solvate has formed by the isolation conditions or purification conditions used to prepare a given compound. Examples of such techniques include thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray crystallography (e.g. single crystal X-ray crystallography or X-ray powder diffraction) and Solid State NMR (SS-NMR, also known as Magic Angle Spinning NMR or MAS-NMR). Such techniques are as much a part of the standard analytical toolkit of the skilled chemist as NMR, IR, HPLC and MS. Alternatively the skilled person can deliberately form a solvate using crystallisation conditions that include an amount of the solvent required for the particular solvate. Thereafter the standard methods described above, can be used to establish whether solvates had formed. Also encompassed by formula (I) are any complexes (e.g. inclusion complexes or clathrates with compounds such as cyclodextrins, or complexes with metals) of the compounds.
Furthermore, the compounds of the present invention may have one or more polymorph (crystalline) or amorphous forms and as such are intended to be included in the scope of the invention.
Compounds of the formula (I) may exist in a number of different geometric isomeric, and tautomeric forms and references to compounds of the formula (I) include all such forms. For the avoidance of doubt, where a compound can exist in one of several geometric isomeric or tautomeric forms and only one is specifically described or shown, all others are nevertheless embraced by formula (I). Other examples of tautomeric forms include, for example, keto-, enol-, and enolate-forms, as in, for example, the following tautomeric pairs: keto/enol (illustrated below), imine/enamine, amide/imino alcohol, amidine/enediamines, nitroso/oxime, thioketone/enethiol, and nitro/aci-nitro.




Where compounds of the formula (I) contain one or more chiral centres, and can exist in the form of two or more optical isomers, references to compounds of the formula (I) include all optical isomeric forms thereof (e.g. enantiomers, epimers and diastereoisomers), either as individual optical isomers, or mixtures (e.g. racemic mixtures) of two or more optical isomers, unless the context requires otherwise. The optical isomers may be characterised and identified by their optical activity (i.e. as + and − isomers, or d and l isomers) or they may be characterised in terms of their absolute stereochemistry using the “R and S” nomenclature developed by Cahn, Ingold and Prelog, see Advanced Organic Chemistry by Jerry March, 4th Edition, John Wiley & Sons, New York, 1992, pages 109-114, and see also Cahn, Ingold & Prelog (1966) Angew. Chem. Int. Ed. Engl., 5, 385-415. Optical isomers can be separated by a number of techniques including chiral chromatography (chromatography on a chiral support) and such techniques are well known to the person skilled in the art. As an alternative to chiral chromatography, optical isomers can be separated by forming diastereoisomeric salts with chiral acids such as (+)-tartaric acid, (−)-pyroglutamic acid, (−)-di-toluoyl-L-tartaric acid, (+)-mandelic acid, (−)-malic acid, and (−)-camphorsulphonic, separating the diastereoisomers by preferential crystallisation, and then dissociating the salts to give the individual enantiomer of the free base.
Where compounds of the formula (I) exist as two or more optical isomeric forms, one enantiomer in a pair of enantiomers may exhibit advantages over the other enantiomer, for example, in terms of biological activity. Thus, in certain circumstances, it may be desirable to use as a therapeutic agent only one of a pair of enantiomers, or only one of a plurality of diastereoisomers. Accordingly, the invention provides compositions containing a compound of the formula (I) having one or more chiral centres, wherein at least 55% (e.g. at least 60%, 65%, 70%, 75%, 80%, 85%, 90% or 95%) of the compound of the formula (I) is present as a single optical isomer (e.g. enantiomer or diastereoisomer). In one general embodiment, 99% or more (e.g. substantially all) of the total amount of the compound of the formula (I) may be present as a single optical isomer (e.g. enantiomer or diastereoisomer). When a specific isomeric form is identified (e.g. S configuration, or E isomer), this means that said isomeric form is substantially free of the other isomer(s), i.e. said isomeric form is present in at least 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 99% or more (e.g. substantially all) of the total amount of the compound of the invention.
Whenever, hereinbefore or hereinafter, compounds include the following bond , this indicates that the compound is a single stereoisomer with unknown configuration or a mixture of stereoisomers.
The compounds of the invention include compounds with one or more isotopic substitutions, and a reference to a particular element includes within its scope all isotopes of the element. For example, a reference to hydrogen includes within its scope 1H, 2H (D), and 3H (T). Similarly, references to carbon and oxygen include within their scope respectively 12C, 13C and 14C and 16O and 18O. The isotopes may be radioactive or non-radioactive. In one embodiment of the invention, the compounds contain no radioactive isotopes. Such compounds are preferred for therapeutic use. In another embodiment, however, the compound may contain one or more radioisotopes. Compounds containing such radioisotopes may be useful in a diagnostic context.
Esters such as carboxylic acid esters and acyloxy esters of the compounds of formula (I) bearing a carboxylic acid group or a hydroxyl group are also embraced by formula (I). In one embodiment of the invention, formula (I) includes within its scope esters of compounds of the formula (I) bearing a hydroxyl group. In another embodiment of the invention, formula (I) does not include within its scope esters of compounds of the formula (I) bearing a hydroxyl group. Examples of acyloxy (reverse ester) groups are represented by —OC(═O)R, wherein R is an acyloxy substituent, for example, a C1-7 alkyl group, a C3-20 heterocyclyl group, or a C5-20 aryl group, preferably a C1-7 alkyl group. Particular examples of acyloxy groups include, but are not limited to, —OC(═O)CH3 (acetoxy), —OC(═O)CH2CH3, —OC(═O)C(CH3)3, —OC(═O)Ph, and —OC(═O)CH2Ph.
For example, some prodrugs are esters of the active compound (e.g., a physiologically acceptable metabolically labile ester). By “prodrugs” is meant for example any compound that is converted in vivo into a biologically active compound of the formula (I). During metabolism, the ester group is cleaved to yield the active drug. Such esters may be formed by esterification, for example, of any of the hydroxyl groups in the parent compound, with, where appropriate, prior protection of any other reactive groups present in the parent compound, followed by deprotection if required.
Examples of such metabolically labile esters include C1-6aminoalkyl [e.g., aminoethyl; 2-(N,N-diethylamino)ethyl; 2-(4-morpholino)ethyl); and acyloxy-C1-7alkyl [e.g., acyloxymethyl; acyloxyethyl; pivaloyloxymethyl; acetoxymethyl; 1-acetoxyethyl; 1-(1-methoxy-1-methyl)ethyl-carbonyloxyethyl; 1-(benzoyloxy)ethyl; isopropoxy-carbonyloxymethyl; 1-isopropoxy-carbonyloxyethyl; cyclohexyl-carbonyloxymethyl; 1-cyclohexyl-carbonyloxyethyl; cyclohexyloxy-carbonyloxymethyl; 1-cyclohexyloxy-carbonyloxyethyl; (4-tetrahydropyranyloxy) carbonyloxymethyl; 1-(4-tetrahydropyranyloxy)carbonyloxyethyl; (4-tetrahydropyranyl)carbonyloxymethyl; and 1-(4-tetrahydropyranyl)carbonyloxyethyl]. Also, some prodrugs are activated enzymatically to yield the active compound, or a compound which, upon further chemical reaction, yields the active compound (for example, as in antigen-directed enzyme pro-drug therapy (ADEPT), gene-directed enzyme pro-drug therapy (GDEPT) and ligand-directed enzyme pro-drug therapy (LIDEPT) etc.). For example, the prodrug may be a sugar derivative or other glycoside conjugate, or may be an amino acid ester derivative.
Protein Tyrosine Kinases (PTK)
The compounds of the invention described herein inhibit or modulate the activity of certain tyrosine kinases, and thus the compounds will be useful in the treatment or prophylaxis, in particular the treatment, of disease states or conditions mediated by those tyrosine kinases, in particular FGFR.
FGFR
The fibroblast growth factor (FGF) family of protein tyrosine kinase (PTK) receptors regulates a diverse array of physiologic functions including mitogenesis, wound healing, cell differentiation and angiogenesis, and development. Both normal and malignant cell growth as well as proliferation are affected by changes in local concentration of FGFs, extracellular signalling molecules which act as autocrine as well as paracrine factors. Autocrine FGF signalling may be particularly important in the progression of steroid hormone-dependent cancers to a hormone independent state. FGFs and their receptors are expressed at increased levels in several tissues and cell lines and overexpression is believed to contribute to the malignant phenotype. Furthermore, a number of oncogenes are homologues of genes encoding growth factor receptors, and there is a potential for aberrant activation of FGF-dependent signalling in human pancreatic cancer (Knights et al., Pharmacology and Therapeutics 2010 125:1 (105-117); Korc M. et al Current Cancer Drug Targets 2009 9:5 (639-651)).
The two prototypic members are acidic fibroblast growth factor (aFGF or FGF1) and basic fibroblast growth factor (bFGF or FGF2), and to date, at least twenty distinct FGF family members have been identified. The cellular response to FGFs is transmitted via four types of high affinity transmembrane protein tyrosine-kinase fibroblast growth factor receptors (FGFR) numbered 1 to 4 (FGFR1 to FGFR4).
Disruption of the FGFR1 pathway should affect tumor cell proliferation since this kinase is activated in many tumor types in addition to proliferating endothelial cells. The over-expression and activation of FGFR1 in tumor-associated vasculature has suggested a role for these molecules in tumor angiogenesis.
A recent study has shown a link between FGFR1 expression and tumorigenicity in Classic Lobular Carcinomas (CLC). CLCs account for 10-15% of all breast cancers and, in general, lack p53 and Her2 expression whilst retaining expression of the oestrogen receptor. A gene amplification of 8p12-p11.2 was demonstrated in  ̃50% of CLC cases and this was shown to be linked with an increased expression of FGFR1. Preliminary studies with siRNA directed against FGFR1, or a small molecule inhibitor of the receptor, showed cell lines harbouring this amplification to be particularly sensitive to inhibition of this signalling pathway. Rhabdomyo sarcoma (RMS) is the most common pediatric soft tissue sarcoma likely results from abnormal proliferation and differentiation during skeletal myogenesis. FGFR1 is over-expressed in primary rhabdomyosarcoma tumors and is associated with hypomethylation of a 5′ CpG island and abnormal expression of the AKT1, NOG, and BMP4 genes. FGFR1 has also been linked to squamous lung cancer, colorectal cancer, glioblastoma, astrocytomas, prostate cancer, small cell lung cancer, melanoma, head and neck cancer, thyroid cancer, uterine cancer.
Fibroblast growth factor receptor 2 has high affinity for the acidic and/or basic fibroblast growth factors, as well as the keratinocyte growth factor ligands. Fibroblast growth factor receptor 2 also propagates the potent osteogenic effects of FGFs during osteoblast growth and differentiation. Mutations in fibroblast growth factor receptor 2, leading to complex functional alterations, were shown to induce abnormal ossification of cranial sutures (craniosynostosis), implying a major role of FGFR signalling in intramembraneous bone formation. For example, in Apert (AP) syndrome, characterized by premature cranial suture ossification, most cases are associated with point mutations engendering gain-of-function in fibroblast growth factor receptor 2. In addition, mutation screening in patients with syndromic craniosynostoses indicates that a number of recurrent FGFR2 mutations accounts for severe forms of Pfeiffer syndrome. Particular mutations of FGFR2 include W290C, D321A, Y340C, C342R, C342S, C342W, N549H, K641R in FGFR2.
Several severe abnormalities in human skeletal development, including Apert, Crouzon, Jackson-Weiss, Beare-Stevenson cutis gyrata, and Pfeiffer syndromes are associated with the occurrence of mutations in fibroblast growth factor receptor 2. Most, if not all, cases of Pfeiffer Syndrome (PS) are also caused by de novo mutation of the fibroblast growth factor receptor 2 gene, and it was recently shown that mutations in fibroblast growth factor receptor 2 break one of the cardinal rules governing ligand specificity. Namely, two mutant splice forms of fibroblast growth factor receptor, FGFR2c and FGFR2b, have acquired the ability to bind to and be activated by atypical FGF ligands. This loss of ligand specificity leads to aberrant signalling and suggests that the severe phenotypes of these disease syndromes result from ectopic ligand-dependent activation of fibroblast growth factor receptor 2.
Genetic aberrations of the FGFR3 receptor tyrosine kinase such as chromosomal translocations or point mutations result in ectopically expressed or deregulated, constitutively active, FGFR3 receptors. Such abnormalities are linked to a subset of multiple myelomas and in bladder, hepatocellular, oral squamous cell carcinoma and cervical carcinomas. Accordingly, FGFR3 inhibitors would be useful in the treatment of multiple myeloma, bladder and cervical carcinomas. FGFR3 is also over-expressed in bladder cancer, in particular invasive bladder cancer. FGFR3 is frequently activated by mutation in urothelial carcinoma (UC). Increased expression was associated with mutation (85% of mutant tumors showed high-level expression) but also 42% of tumors with no detectable mutation showed over-expression, including many muscle-invasive tumors. FGFR3 is also linked to endometrial and thyroid cancer.
Over expression of FGFR4 has been linked to poor prognosis in both prostate and thyroid carcinomas. In addition a germline polymorphism (Gly388Arg) is associated with increased incidence of lung, breast, colon, liver (HCC) and prostate cancers. In addition, a truncated form of FGFR4 (including the kinase domain) has also been found to be present in 40% of pituitary tumours but not present in normal tissue. FGFR4 overexpression has been observed in liver, colon and lung tumours. FGFR4 has been implicated in colorectal and liver cancer where expression of its ligand FGF19 is frequently elevated. FGFR4 is also linked to astrocytomas, rhabdomyosarcoma.
Fibrotic conditions are a major medical problem resulting from abnormal or excessive deposition of fibrous tissue. This occurs in many diseases, including liver cirrhosis, glomerulonephritis, pulmonary fibrosis, systemic fibrosis, rheumatoid arthritis, as well as the natural process of wound healing. The mechanisms of pathological fibrosis are not fully understood but are thought to result from the actions of various cytokines (including tumor necrosis factor (TNF), fibroblast growth factors (FGF's), platelet derived growth factor (PDGF) and transforming growth factor beta. (TGFβ) involved in the proliferation of fibroblasts and the deposition of extracellular matrix proteins (including collagen and fibronectin). This results in alteration of tissue structure and function and subsequent pathology.
A number of preclinical studies have demonstrated the up-regulation of fibroblast growth factors in preclinical models of lung fibrosis. TGFβ1 and PDGF have been reported to be involved in the fibrogenic process and further published work suggests the elevation of FGF's and consequent increase in fibroblast proliferation, may be in response to elevated TGFβ1. The potential therapeutic benefit of targeting the fibrotic mechanism in conditions such as idiopathic pulmonary fibrosis (IPF) is suggested by the reported clinical effect of the anti-fibrotic agent pirfenidone. Idiopathic pulmonary fibrosis (also referred to as Cryptogenic fibrosing alveolitis) is a progressive condition involving scarring of the lung. Gradually, the air sacs of the lungs become replaced by fibrotic tissue, which becomes thicker, causing an irreversible loss of the tissue's ability to transfer oxygen into the bloodstream. The symptoms of the condition include shortness of breath, chronic dry coughing, fatigue, chest pain and loss of appetite resulting in rapid weight loss. The condition is extremely serious with approximately 50% mortality after 5 years.
As such, the compounds which inhibit FGFR will be useful in providing a means of preventing the growth or inducing apoptosis in tumours, particularly by inhibiting angiogenesis. It is therefore anticipated that the compounds will prove useful in treating or preventing proliferative disorders such as cancers. In particular tumours with activating mutants of receptor tyrosine kinases (RTK) or upregulation of receptor tyrosine kinases may be particularly sensitive to the inhibitors. Patients with activating mutants of any of the iso forms of the specific RTKs discussed herein may also find treatment with RTK inhibitors particularly beneficial, for instance patients with tumors, e.g. bladder or brain tumors, with FGFR3-TACC3 translocation.
Vascular Endothelial Growth Factor Receptor (VEGFR)
Chronic proliferative diseases are often accompanied by profound angiogenesis, which can contribute to or maintain an inflammatory and/or proliferative state, or which leads to tissue destruction through the invasive proliferation of blood vessels.
Angiogenesis is generally used to describe the development of new or replacement blood vessels, or neovascularisation. It is a necessary and physiological normal process by which vasculature is established in the embryo. Angiogenesis does not occur, in general, in most normal adult tissues, exceptions being sites of ovulation, menses and wound healing. Many diseases, however, are characterized by persistent and unregulated angiogenesis. For instance, in arthritis, new capillary blood vessels invade the joint and destroy cartilage. In diabetes (and in many different eye diseases), new vessels invade the macula or retina or other ocular structures, and may cause blindness. The process of atherosclerosis has been linked to angiogenesis. Tumor growth and metastasis have been found to be angiogenesis-dependent.
The recognition of the involvement of angiogenesis in major diseases has been accompanied by research to identify and develop inhibitors of angiogenesis. These inhibitors are generally classified in response to discrete targets in the angiogenesis cascade, such as activation of endothelial cells by an angiogenic signal; synthesis and release of degradative enzymes; endothelial cell migration; proliferation of endothelial cells; and formation of capillary tubules. Therefore, angiogenesis occurs in many stages and attempts are underway to discover and develop compounds that work to block angiogenesis at these various stages.
There are publications that teach that inhibitors of angiogenesis, working by diverse mechanisms, are beneficial in diseases such as cancer and metastasis, ocular diseases, arthritis and hemangioma.
Vascular endothelial growth factor (VEGF), a polypeptide, is mitogenic for endothelial cells in vitro and stimulates angiogenic responses in vivo. VEGF has also been linked to inappropriate angiogenesis. VEGFR(s) are protein tyrosine kinases (PTKs). PTKs catalyze the phosphorylation of specific tyrosine residues in proteins involved in cell function thus regulating cell growth, survival and differentiation.
Three PTK receptors for VEGF have been identified: VEGFR-1 (Flt-1); VEGFR-2 (Flk-1 or KDR) and VEGFR-3 (Flt-4). These receptors are involved in angiogenesis and participate in signal transduction. Of particular interest is VEGFR-2, which is a transmembrane receptor PTK expressed primarily in endothelial cells. Activation of VEGFR-2 by VEGF is a critical step in the signal transduction pathway that initiates tumour angiogenesis. VEGF expression may be constitutive to tumour cells and can also be upregulated in response to certain stimuli. One such stimuli is hypoxia, where VEGF expression is upregulated in both tumour and associated host tissues. The VEGF ligand activates VEGFR-2 by binding with its extracellular VEGF binding site. This leads to receptor dimerization of VEGFRs and autophosphorylation of tyrosine residues at the intracellular kinase domain of VEGFR-2. The kinase domain operates to transfer a phosphate from ATP to the tyrosine residues, thus providing binding sites for signalling proteins downstream of VEGFR-2 leading ultimately to initiation of angiogenesis.
Inhibition at the kinase domain binding site of VEGFR-2 would block phosphorylation of tyrosine residues and serve to disrupt initiation of angiogenesis.
Angiogenesis is a physiologic process of new blood vessel formation mediated by various cytokines called angiogenic factors. Although its potential pathophysiologic role in solid tumors has been extensively studied for more than 3 decades, enhancement of angiogenesis in chronic lymphocytic leukemia (CLL) and other malignant hematological disorders has been recognized more recently. An increased level of angiogenesis has been documented by various experimental methods both in bone marrow and lymph nodes of patients with CLL. Although the role of angiogenesis in the pathophysiology of this disease remains to be fully elucidated, experimental data suggest that several angiogenic factors play a role in the disease progression. Biologic markers of angiogenesis were also shown to be of prognostic relevance in CLL. This indicates that VEGFR inhibitors may also be of benefit for patients with leukemia's such as CLL.
In order for a tumour mass to get beyond a critical size, it must develop an associated vasculature. It has been proposed that targeting a tumor vasculature would limit tumor expansion and could be a useful cancer therapy. Observations of tumor growth have indicated that small tumour masses can persist in a tissue without any tumour-specific vasculature. The growth arrest of nonvascularized tumors has been attributed to the effects of hypoxia at the center of the tumor. More recently, a variety of proangiogenic and antiangiogenic factors have been identified and have led to the concept of the “angiogenic switch,” a process in which disruption of the normal ratio of angiogenic stimuli and inhibitors in a tumor mass allows for autonomous vascularization. The angiogenic switch appears to be governed by the same genetic alterations that drive malignant conversion: the activation of oncogenes and the loss of tumour suppressor genes. Several growth factors act as positive regulators of angiogenesis. Foremost among these are vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and angiogenin. Proteins such as thrombospondin (Tsp-1), angiostatin, and endostatin function as negative regulators of angiogenesis.
Inhibition of VEGFR2 but not VEGFR1 markedly disrupts angiogenic switching, persistent angiogenesis, and initial tumor growth in a mouse model. In late-stage tumors, phenotypic resistance to VEGFR2 blockade emerged, as tumors regrew during treatment after an initial period of growth suppression. This resistance to VEGF blockade involves reactivation of tumour angiogenesis, independent of VEGF and associated with hypoxia-mediated induction of other proangiogenic factors, including members of the FGF family. These other proangiogenic signals are functionally implicated in the revascularization and regrowth of tumours in the evasion phase, as FGF blockade impairs progression in the face of VEGF inhibition.
There is evidence for normalization of glioblastoma blood vessels in patients treated with a pan-VEGF receptor tyrosine kinase inhibitor, AZD2171, in a phase 2 study. MRI determination of vessel normalization in combination with circulating biomarkers provides for an effective means to assess response to antiangiogenic agents.
PDGFR
A malignant tumour is the product of uncontrolled cell proliferation. Cell growth is controlled by a delicate balance between growth-promoting and growth-inhibiting factors. In normal tissue the production and activity of these factors results in differentiated cells growing in a controlled and regulated manner that maintains the normal integrity and functioning of the organ. The malignant cell has evaded this control; the natural balance is disturbed (via a variety of mechanisms) and unregulated, aberrant cell growth occurs. A growth factor of importance in tumour development is the platelet-derived growth factor (PDGF) that comprises a family of peptide growth factors that signal through cell surface tyrosine kinase receptors (PDGFR) and stimulate various cellular functions including growth, proliferation, and differentiation.
Advantages of a Selective Inhibitor
Development of FGFR kinase inhibitors with a differentiated selectivity profile provides a new opportunity to use these targeted agents in patient sub-groups whose disease is driven by FGFR deregulation. Compounds that exhibit reduced inhibitory action on additional kinases, particularly VEGFR2 and PDGFR-beta, offer the opportunity to have a differentiated side-effect or toxicity profile and as such allow for a more effective treatment of these indications. Inhibitors of VEGFR2 and PDGFR-beta are associated with toxicities such as hypertension or oedema respectively. In the case of VEGFR2 inhibitors this hypertensive effect is often dose limiting, may be contraindicated in certain patient populations and requires clinical management.
Biological Activity and Therapeutic Uses
The compounds of the invention, and subgroups thereof, have fibroblast growth factor receptor (FGFR) inhibiting or modulating activity and/or vascular endothelial growth factor receptor (VEGFR) inhibiting or modulating activity, and/or platelet derived growth factor receptor (PDGFR) inhibiting or modulating activity, and which will be useful in preventing or treating disease states or conditions described herein. In addition the compounds of the invention, and subgroups thereof, will be useful in preventing or treating diseases or condition mediated by the kinases. References to the preventing or prophylaxis or treatment of a disease state or condition such as cancer include within their scope alleviating or reducing the incidence of cancer.
As used herein, the term “modulation”, as applied to the activity of a kinase, is intended to define a change in the level of biological activity of the protein kinase. Thus, modulation encompasses physiological changes which effect an increase or decrease in the relevant protein kinase activity. In the latter case, the modulation may be described as “inhibition”. The modulation may arise directly or indirectly, and may be mediated by any mechanism and at any physiological level, including for example at the level of gene expression (including for example transcription, translation and/or post-translational modification), at the level of expression of genes encoding regulatory elements which act directly or indirectly on the levels of kinase activity. Thus, modulation may imply elevated/suppressed expression or over- or under-expression of a kinase, including gene amplification (i.e. multiple gene copies) and/or increased or decreased expression by a transcriptional effect, as well as hyper- (or hypo-)activity and (de)activation of the protein kinase(s) (including (de)activation) by mutation(s). The terms “modulated”, “modulating” and “modulate” are to be interpreted accordingly.
As used herein, the term “mediated”, as used e.g. in conjunction with a kinase as described herein (and applied for example to various physiological processes, diseases, states, conditions, therapies, treatments or interventions) is intended to operate limitatively so that the various processes, diseases, states, conditions, treatments and interventions to which the term is applied are those in which the kinase plays a biological role. In cases where the term is applied to a disease, state or condition, the biological role played by a kinase may be direct or indirect and may be necessary and/or sufficient for the manifestation of the symptoms of the disease, state or condition (or its aetiology or progression). Thus, kinase activity (and in particular aberrant levels of kinase activity, e.g. kinase over-expression) need not necessarily be the proximal cause of the disease, state or condition: rather, it is contemplated that the kinase mediated diseases, states or conditions include those having multifactoral aetiologies and complex progressions in which the kinase in question is only partially involved. In cases where the term is applied to treatment, prophylaxis or intervention, the role played by the kinase may be direct or indirect and may be necessary and/or sufficient for the operation of the treatment, prophylaxis or outcome of the intervention. Thus, a disease state or condition mediated by a kinase includes the development of resistance to any particular cancer drug or treatment.
Thus, for example, the compounds of the invention may be useful in alleviating or reducing the incidence of cancer.
More particularly, the compounds of the formulae (I) and sub-groups thereof are inhibitors of FGFRs. For example, compounds of the invention have activity against FGFR1, FGFR2, FGFR3, and/or FGFR4, and in particular FGFRs selected from FGFR1, FGFR2 and FGFR3; or in particular the compounds of formula (I) and sub-groups thereof are inhibitors of FGFR4.
Preferred compounds are compounds that inhibit one or more FGFR selected from FGFR1, FGFR2, FGFR3, and FGFR4. Preferred compounds of the invention are those having IC50 values of less than 0.1 μM.
Compounds of the invention also have activity against VEGFR.
In addition many of the compounds of the invention exhibit selectivity for the FGFR 1, 2, and/or 3, and/or 4 compared to VEGFR (in particular VEGFR2) and/or PDGFR and such compounds represent one preferred embodiment of the invention. In particular, the compounds exhibit selectivity over VEGFR2. For example, many compounds of the invention have IC50 values against FGFR1, 2 and/or 3 and/or 4 that are between a tenth and a hundredth of the IC50 against VEGFR (in particular VEGFR2) and/or PDGFR B. In particular preferred compounds of the invention have at least 10 times greater activity against or inhibition of FGFR in particular FGFR1, FGFR2, FGFR3 and/or FGFR4 than VEGFR2. More preferably the compounds of the invention have at least 100 times greater activity against or inhibition of FGFR in particular FGFR1, FGFR2, FGFR3 and/or FGFR4 than VEGFR2. This can be determined using the methods described herein.
As a consequence of their activity in modulating or inhibiting FGFR, and/or VEGFR kinases, the compounds will be useful in providing a means of preventing the growth or inducing apoptosis of neoplasias, particularly by inhibiting angiogenesis. It is therefore anticipated that the compounds will prove useful in treating or preventing proliferative disorders such as cancers. In addition, the compounds of the invention could be useful in the treatment of diseases in which there is a disorder of proliferation, apoptosis or differentiation.
In particular tumours with activating mutants of VEGFR or upregulation of VEGFR and patients with elevated levels of serum lactate dehydrogenase may be particularly sensitive to the compounds of the invention. Patients with activating mutants of any of the isoforms of the specific RTKs discussed herein may also find treatment with the compounds of the invention particularly beneficial. For example, VEGFR overexpression in acute leukemia cells where the clonal progenitor may express VEGFR. Also, particular tumours with activating mutants or upregulation or overexpression of any of the isoforms of FGFR such as FGFR1, FGFR2 or FGFR3 or FGFR4 may be particularly sensitive to the compounds of the invention and thus patients as discussed herein with such particular tumours may also find treatment with the compounds of the invention particularly beneficial. It may be preferred that the treatment is related to or directed at a mutated form of one of the receptor tyrosine kinases, such as discussed herein. Diagnosis of tumours with such mutations could be performed using techniques known to a person skilled in the art and as described herein such as RTPCR and FISH.
Examples of cancers which may be treated (or inhibited) include, but are not limited to, a carcinoma, for example a carcinoma of the bladder, breast, colon (e.g. colorectal carcinomas such as colon adenocarcinoma and colon adenoma), kidney, urothelial, uterus, epidermis, liver, lung (for example adenocarcinoma, small cell lung cancer and non-small cell lung carcinomas, squamous lung cancer), oesophagus, head and neck, gall bladder, ovary, pancreas (e.g. exocrine pancreatic carcinoma), stomach, gastrointestinal (also known as gastric) cancer (e.g. gastrointestinal stromal tumours), cervix, endometrium, thyroid, prostate, or skin (for example squamous cell carcinoma or dermatofibrosarcoma protuberans); pituitary cancer, a hematopoietic tumour of lymphoid lineage, for example leukemia, acute lymphocytic leukemia, chronic lymphocytic leukemia, B-cell lymphoma (e.g. diffuse large B-cell lymphoma), T-cell lymphoma, Hodgkin's lymphoma, non-Hodgkin's lymphoma, hairy cell lymphoma, or Burkett's lymphoma; a hematopoietic tumour of myeloid lineage, for example leukemias, acute and chronic myelogenous leukemias, chronic myelomonocytic leukemia (CMML), myeloproliferative disorder, myeloproliferative syndrome, myelodysplastic syndrome, or promyelocytic leukemia; multiple myeloma; thyroid follicular cancer; hepatocellular cancer, a tumour of mesenchymal origin (e.g. Ewing's sarcoma), for example fibrosarcoma or rhabdomyo sarcoma; a tumour of the central or peripheral nervous system, for example astrocytoma, neuroblastoma, glioma (such as glioblastoma multiforme) or schwannoma; melanoma; seminoma; teratocarcinoma; osteosarcoma; xeroderma pigmentosum; keratoctanthoma; thyroid follicular cancer; or Kaposi's sarcoma. In particular, squamous lung cancer, breast cancer, colorectal cancer, glioblastoma, astrocytomas, prostate cancer, small cell lung cancer, melanoma, head and neck cancer, thyroid cancer, uterine cancer, gastric cancer, hepatocellular cancer, cervix cancer, multiple myeloma, bladder cancer, endometrial cancer, urothelial cancer, colon cancer, rhabdomyosarcoma, pituitary gland cancer.
Examples of cancers which may be treated (or inhibited) include, but are not limited to, bladder cancer, urothelial cancer, metastatic urothelial cancer, surgically unresectable urothelial cancer, breast cancer, glioblastoma, lung cancer, non small cell lung cancer, squamous cell lung cancer, adenocarcinoma of the lung, pulmonary adenocarcinoma, small cell lung cancer, ovarian cancer, endometrial cancer, cervical cancer, soft tissue sarcoma, head and neck squamous cell carcinoma, gastric cancer, oesophageal cancer, squamous cell carcinoma of the oesophagus, adenocarcinoma of the oesophagus, cholangiocarcinoma, hepatocellular carcinoma.
Certain cancers are resistant to treatment with particular drugs. This can be due to the type of the tumour or can arise due to treatment with the compound. In this regard, references to multiple myeloma includes bortezomib sensitive multiple myeloma or refractory multiple myeloma. Similarly, references to chronic myelogenous leukemia includes imitanib sensitive chronic myelogenous leukemia and refractory chronic myelogenous leukemia. Chronic myelogenous leukemia is also known as chronic myeloid leukemia, chronic granulocytic leukemia or CML. Likewise, acute myelogenous leukemia, is also called acute myeloblastic leukemia, acute granulocytic leukemia, acute nonlymphocytic leukaemia or AML.
The compounds of the invention can also be used in the treatment of hematopoetic diseases of abnormal cell proliferation whether pre-malignant or stable such as myeloproliferative diseases. Myeloproliferative diseases (“MPD”s) are a group of diseases of the bone marrow in which excess cells are produced. They are related to, and may evolve into, myelodysplastic syndrome. Myeloproliferative diseases include polycythemia vera, essential thrombocythemia and primary myelofibrosis. A further haematological disorder is hypereosinophilic syndrome. T-cell lymphoproliferative diseases include those derived from natural Killer cells.
In addition the compounds of the invention can be used to gastrointestinal (also known as gastric) cancer e.g. gastrointestinal stromal tumours. Gastrointestinal cancer refers to malignant conditions of the gastrointestinal tract, including the esophagus, stomach, liver, biliary system, pancreas, bowels, and anus.
Thus, in the pharmaceutical compositions, uses or methods of this invention for treating a disease or condition comprising abnormal cell growth, the disease or condition comprising abnormal cell growth in one embodiment is a cancer.
Particular subsets of cancers include multiple myeloma, bladder, cervical, prostate and thyroid carcinomas, lung, breast, and colon cancers.
A further subset of cancers includes multiple myeloma, bladder, hepatocellular, oral squamous cell carcinoma and cervical carcinomas.
The compound of the invention, having FGFR such as FGFR1 inhibitory activity, may be particularly useful in the treatment or prevention of breast cancer in particular Classic Lobular Carcinomas (CLC).
As the compounds of the invention have FGFR4 activity they will also be useful in the treatment of prostate or pituitary cancers, or they will be useful in the treatment of breast cancer, lung cancer, prostate cancer, liver cancer (HCC) or lung cancer.
In particular the compounds of the invention as FGFR inhibitors, are useful in the treatment of multiple myeloma, myeloproliferative disorders, endometrial cancer, prostate cancer, bladder cancer, lung cancer, ovarian cancer, breast cancer, gastric cancer, colorectal cancer, and oral squamous cell carcinoma.
Further subsets of cancer are multiple myeloma, endometrial cancer, bladder cancer, cervical cancer, prostate cancer, lung cancer, breast cancer, colorectal cancer and thyroid carcinomas.
In particular the compounds of the invention are useful in the treatment of multiple myeloma (in particular multiple myeloma with t(4;14) translocation or overexpressing FGFR3), prostate cancer (hormone refractory prostrate carcinomas), endometrial cancer (in particular endometrial tumours with activating mutations in FGFR2) and breast cancer (in particular lobular breast cancer).
In particular the compounds are useful in the treatment of lobular carcinomas such as CLC (Classic lobular carcinoma).
As the compounds have activity against FGFR3 they will be useful in the treatment of multiple myeloma and bladder cancer.
In particular, the compounds have activity against tumours with FGFR3-TACC3 translocation, in particular bladder or brain tumours with FGFR3-TACC3 translocation.
In particular the compounds are useful for the treatment of t(4;14) translocation positive multiple myeloma.
In one embodiment the compounds may be useful for the treatment of sarcoma. In one embodiment the compounds may be useful for the treatment of lung cancer, e.g. squamous cell carcinoma.
As the compounds have activity against FGFR2 they will be useful in the treatment of endometrial, ovarian, gastric, hepatocellular, uterine, cervix and colorectal cancers. FGFR2 is also overexpressed in epithelial ovarian cancer, therefore the compounds of the invention may be specifically useful in treating ovarian cancer such as epithelial ovarian cancer.
In one embodiment, the compounds may be useful for the treatment of lung cancer, in particular NSCLC, squamous cell carcinoma, liver cancer, kidney cancer, breast cancer, colon cancer, colorectal cancer, prostate cancer.
Compounds of the invention may also be useful in the treatment of tumours pre-treated with VEGFR2 inhibitor or VEGFR2 antibody (e.g. Avastin).
In particular the compounds of the invention may be useful in the treatment of VEGFR2-resistant tumours. VEGFR2 inhibitors and antibodies are used in the treatment of thyroid and renal cell carcinomas, therefore the compounds of the invention may be useful in the treatment of VEGFR2-resistant thyroid and renal cell carcinomas.
The cancers may be cancers which are sensitive to inhibition of any one or more FGFRs selected from FGFR1, FGFR2, FGFR3, FGFR4, for example, one or more FGFRs selected from FGFR1, FGFR2 or FGFR3.
Whether or not a particular cancer is one which is sensitive to inhibition of FGFR or VEGFR signalling may be determined by means of a cell growth assay as set out below or by a method as set out in the section headed “Methods of Diagnosis”.
The compounds of the invention, and in particular those compounds having FGFR, or VEGFR inhibitory activity, may be particularly useful in the treatment or prevention of cancers of a type associated with or characterised by the presence of elevated levels of FGFR, or VEGFR, for example the cancers referred to in this context in the introductory section of this application.
The compounds of the present invention may be useful for the treatment of the adult population. The compounds of the present invention may be useful for the treatment of the pediatric population.
It has been discovered that some FGFR inhibitors can be used in combination with other anticancer agents. For example, it may be beneficial to combine an inhibitor that induces apoptosis with another agent which acts via a different mechanism to regulate cell growth thus treating two of the characteristic features of cancer development. Examples of such combinations are set out below.
The compounds of the invention may be useful in treating other conditions which result from disorders in proliferation such as type II or non-insulin dependent diabetes mellitus, autoimmune diseases, head trauma, stroke, epilepsy, neurodegenerative diseases such as Alzheimer's, motor neurone disease, progressive supranuclear palsy, corticobasal degeneration and Pick's disease for example autoimmune diseases and neurodegenerative diseases.
One sub-group of disease states and conditions that the compounds of the invention may be useful consists of inflammatory diseases, cardiovascular diseases and wound healing.
FGFR, and VEGFR are also known to play a role in apoptosis, angiogenesis, proliferation, differentiation and transcription and therefore the compounds of the invention could also be useful in the treatment of the following diseases other than cancer; chronic inflammatory diseases, for example systemic lupus erythematosus, autoimmune mediated glomerulonephritis, rheumatoid arthritis, psoriasis, inflammatory bowel disease, autoimmune diabetes mellitus, Eczema hypersensitivity reactions, asthma, COPD, rhinitis, and upper respiratory tract disease; cardiovascular diseases for example cardiac hypertrophy, restenosis, atherosclerosis; neurodegenerative disorders, for example Alzheimer's disease, AIDS-related dementia, Parkinson's disease, amyotropic lateral sclerosis, retinitis pigmentosa, spinal muscular atropy and cerebellar degeneration; glomerulonephritis; myelodysplastic syndromes, ischemic injury associated myocardial infarctions, stroke and reperfusion injury, arrhythmia, atherosclerosis, toxin-induced or alcohol related liver diseases, haematological diseases, for example, chronic anemia and aplastic anemia; degenerative diseases of the musculoskeletal system, for example, osteoporosis and arthritis, aspirin-sensitive rhinosinusitis, cystic fibrosis, multiple sclerosis, kidney diseases and cancer pain.
In addition, mutations of FGFR2 are associated with several severe abnormalities in human skeletal development and thus the compounds of invention could be useful in the treatment of abnormalities in human skeletal development, including abnormal ossification of cranial sutures (craniosynostosis), Apert (AP) syndrome, Crouzon syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrate syndrome, and Pfeiffer syndrome.
The compound of the invention, having FGFR such as FGFR2 or FGFR3 inhibitory activity, may be particularly useful in the treatment or prevention of the skeletal diseases. Particular skeletal diseases are achondroplasia or thanatophoric dwarfism (also known as thanatophoric dysplasia).
The compound of the invention, having FGFR such as FGFR1, FGFR2 or FGFR3 inhibitory activity, may be particularly useful in the treatment or prevention in pathologies in which progressive fibrosis is a symptom. Fibrotic conditions in which the compounds of the inventions may be useful in the treatment of include diseases exhibiting abnormal or excessive deposition of fibrous tissue for example in liver cirrhosis, glomerulonephritis, pulmonary fibrosis, systemic fibrosis, rheumatoid arthritis, as well as the natural process of wound healing. In particular the compounds of the inventions may also be useful in the treatment of lung fibrosis in particular in idiopathic pulmonary fibrosis.
The over-expression and activation of FGFR and VEGFR in tumor-associated vasculature has also suggested a role for compounds of the invention in preventing and disrupting initiation of tumor angiogenesis. In particular the compounds of the invention may be useful in the treatment of cancer, metastasis, leukemia's such as CLL, ocular diseases such as age-related macular degeneration in particular wet form of age-related macular degeneration, ischemic proliferative retinopathies such as retinopathy of prematurity (ROP) and diabetic retinopathy, rheumatoid arthritis and hemangioma.
The activity of the compounds of the invention as inhibitors of FGFR1-4, VEGFR and/or PDGFR A/B can be measured using the assays set forth in the examples below and the level of activity exhibited by a given compound can be defined in terms of the IC50 value. Preferred compounds of the present invention are compounds having an IC50 value of less than 1 μM, more preferably less than 0.1 μM.
The invention provides compounds that have FGFR inhibiting or modulating activity, and which may be useful in preventing or treating disease states or conditions mediated by FGFR kinases.
In one embodiment, there is provided a compound as defined herein for use in therapy, for use as a medicine. In a further embodiment, there is provided a compound as defined herein for use in the prophylaxis or treatment, in particular in the treatment, of a disease state or condition mediated by a FGFR kinase.
Thus, for example, the compounds of the invention may be useful in alleviating or reducing the incidence of cancer. Therefore, in a further embodiment, there is provided a compound as defined herein for use in the prophylaxis or treatment, in particular the treatment, of cancer. In one embodiment, the compound as defined herein is for use in the prophylaxis or treatment of FGFR-dependent cancer. In one embodiment, the compound as defined herein is for use in the prophylaxis or treatment of cancer mediated by FGFR kinases.
Accordingly, the invention provides inter alia:

    
    
        A method for the prophylaxis or treatment of a disease state or condition mediated by a FGFR kinase, which method comprises administering to a subject in need thereof a compound of the formula (I) as defined herein.
        A method for the prophylaxis or treatment of a disease state or condition as described herein, which method comprises administering to a subject in need thereof a compound of the formula (I) as defined herein.
        A method for the prophylaxis or treatment of cancer, which method comprises administering to a subject in need thereof a compound of the formula (I) as defined herein.
        A method for alleviating or reducing the incidence of a disease state or condition mediated by a FGFR kinase, which method comprises administering to a subject in need thereof a compound of the formula (I) as defined herein.
        A method of inhibiting a FGFR kinase, which method comprises contacting the kinase with a kinase-inhibiting compound of the formula (I) as defined herein.
        A method of modulating a cellular process (for example cell division) by inhibiting the activity of a FGFR kinase using a compound of the formula (I) as defined herein.
        A compound of formula (I) as defined herein for use as a modulator of a cellular process (for example cell division) by inhibiting the activity of a FGFR kinase.
        A compound of formula (I) as defined herein for use in the prophylaxis or treatment of cancer, in particular the treatment of cancer.
        A compound of formula (I) as defined herein for use as a modulator (e.g. inhibitor) of FGFR.
        The use of a compound of formula (I) as defined herein for the manufacture of a medicament for the prophylaxis or treatment of a disease state or condition mediated by a FGFR kinase, the compound having the formula (I) as defined herein.
        The use of a compound of formula (I) as defined herein for the manufacture of a medicament for the prophylaxis or treatment of a disease state or condition as described herein.
        The use of a compound of formula (I) as defined herein for the manufacture of a medicament for the prophylaxis or treatment, in particular the treatment, of cancer.
        The use of a compound of formula (I) as defined herein for the manufacture of a medicament for modulating (e.g. inhibiting) the activity of FGFR.
        Use of a compound of formula (I) as defined herein in the manufacture of a medicament for modulating a cellular process (for example cell division) by inhibiting the activity of a FGFR kinase.
        The use of a compound of the formula (I) as defined herein for the manufacture of a medicament for prophylaxis or treatment of a disease or condition characterised by up-regulation of a FGFR kinase (e.g. FGFR1 or FGFR2 or FGFR3 or FGFR4).
        The use of a compound of the formula (I) as defined herein for the manufacture of a medicament for the prophylaxis or treatment of a cancer, the cancer being one which is characterised by up-regulation of a FGFR kinase (e.g. FGFR1 or FGFR2 or FGFR3 or FGFR4).
        The use of a compound of the formula (I) as defined herein for the manufacture of a medicament for the prophylaxis or treatment of cancer in a patient selected from a sub-population possessing a genetic aberrations of FGFR3 kinase.
        The use of a compound of the formula (I) as defined herein for the manufacture of a medicament for the prophylaxis or treatment of cancer in a patient who has been diagnosed as forming part of a sub-population possessing a genetic aberrations of FGFR3 kinase.
        A method for the prophylaxis or treatment of a disease or condition characterised by up-regulation of a FGFR kinase (e.g. FGFR1 or FGFR2 or FGFR3 or FGFR4), the method comprising administering a compound of the formula (I) as defined herein.
        A method for alleviating or reducing the incidence of a disease or condition characterised by up-regulation of a FGFR kinase (e.g. FGFR1 or FGFR2 or FGFR3 or FGFR4), the method comprising administering a compound of the formula (I) as defined herein.
        A method for the prophylaxis or treatment of (or alleviating or reducing the incidence of) cancer in a patient suffering from or suspected of suffering from cancer; which method comprises (i) subjecting a patient to a diagnostic test to determine whether the patient possesses a genetic aberrations of FGFR3 gene; and (ii) where the patient does possess the said variant, thereafter administering to the patient a compound of the formula (I) as defined herein having FGFR3 kinase inhibiting activity.
        A method for the prophylaxis or treatment of (or alleviating or reducing the incidence of) a disease state or condition characterised by up-regulation of an FGFR kinase (e.g. FGFR1 or FGFR2 or FGFR3 or FGFR4); which method comprises (i) subjecting a patient to a diagnostic test to detect a marker characteristic of up-regulation of a FGFR kinase (e.g. FGFR1 or FGFR2 or FGFR3 or FGFR4) and (ii) where the diagnostic test is indicative of up-regulation of a FGFR kinase, thereafter administering to the patient a compound of the formula (I) as defined herein having FGFR kinase inhibiting activity.
    
    


In one embodiment, the disease mediated by FGFR kinases is a oncology related disease (e.g. cancer). In one embodiment, the disease mediated by FGFR kinases is a non-oncology related disease (e.g. any disease disclosed herein excluding cancer). In one embodiment the disease mediated by FGFR kinases is a condition described herein. In one embodiment the disease mediated by FGFR kinases is a skeletal condition described herein. Particular abnormalities in human skeletal development, include abnormal ossification of cranial sutures (craniosynostosis), Apert (AP) syndrome, Crouzon syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrate syndrome, Pfeiffer syndrome, achondroplasia and thanatophoric dwarfism (also known as thanatophoric dysplasia).
Mutated Kinases
Drug resistant kinase mutations can arise in patient populations treated with kinase inhibitors. These occur, in part, in the regions of the protein that bind to or interact with the particular inhibitor used in therapy. Such mutations reduce or increase the capacity of the inhibitor to bind to and inhibit the kinase in question. This can occur at any of the amino acid residues which interact with the inhibitor or are important for supporting the binding of said inhibitor to the target. An inhibitor that binds to a target kinase without requiring the interaction with the mutated amino acid residue will likely be unaffected by the mutation and will remain an effective inhibitor of the enzyme.
A study in gastric cancer patient samples showed the presence of two mutations in FGFR2, Ser167Pro in exon IIIa and a splice site mutation 940-2A-G in exon IIIc. These mutations are identical to the germline activating mutations that cause craniosynotosis syndromes and were observed in 13% of primary gastric cancer tissues studied. In addition activating mutations in FGFR3 were observed in 5% of the patient samples tested and overexpression of FGFRs has been correlated with a poor prognosis in this patient group.
In addition there are chromosomal translocations or point mutations that have been observed in FGFR which give rise to gain-of-function, over-expressed, or constitutively active biological states.
The compounds of the invention would therefore find particular application in relation to cancers which express a mutated molecular target such as FGFR. Diagnosis of tumours with such mutations could be performed using techniques known to a person skilled in the art and as described herein such as RTPCR and FISH.
It has been suggested that mutations of a conserved threonine residue at the ATP binding site of FGFR would result in inhibitor resistance. The amino acid valine 561 has been mutated to a methionine in FGFR1 which corresponds to previously reported mutations found in Abl (T315) and EGFR (T766) that have been shown to confer resistance to selective inhibitors. Assay data for FGFR1 V561M showed that this mutation conferred resistance to a tyrosine kinase inhibitor compared to that of the wild type.
Methods of Diagnosis
Prior to administration of a compound of the formula (I), a patient may be screened to determine whether a disease or condition from which the patient is or may be suffering is one which would be susceptible to treatment with a compound having activity against FGFR, and/or VEGFR.
For example, a biological sample taken from a patient may be analysed to determine whether a condition or disease, such as cancer, that the patient is or may be suffering from is one which is characterised by a genetic abnormality or abnormal protein expression which leads to up-regulation of the levels or activity of FGFR, and/or VEGFR or to sensitisation of a pathway to normal FGFR, and/or VEGFR activity, or to upregulation of these growth factor signalling pathways such as growth factor ligand levels or growth factor ligand activity or to upregulation of a biochemical pathway downstream of FGFR, and/or VEGFR activation.
Examples of such abnormalities that result in activation or sensitisation of the FGFR, and/or VEGFR signal include loss of, or inhibition of apoptotic pathways, up-regulation of the receptors or ligands, or presence of mutant variants of the receptors or ligands e.g PTK variants. Tumours with mutants of FGFR1, FGFR2 or FGFR3 or FGFR4 or up-regulation, in particular over-expression of FGFR1, or gain-of-function mutants of FGFR2 or FGFR3 may be particularly sensitive to FGFR inhibitors.
For example, point mutations engendering gain-of-function in FGFR2 have been identified in a number of conditions. In particular activating mutations in FGFR2 have been identified in 10% of endometrial tumours.
In addition, genetic aberrations of the FGFR3 receptor tyrosine kinase such as chromosomal translocations or point mutations resulting in ectopically expressed or deregulated, constitutively active, FGFR3 receptors have been identified and are linked to a subset of multiple myelomas, bladder and cervical carcinomas. A particular mutation T674I of the PDGF receptor has been identified in imatinib-treated patients. In addition, a gene amplification of 8p12-p11.2 was demonstrated in  ̃50% of lobular breast cancer (CLC) cases and this was shown to be linked with an increased expression of FGFR1. Preliminary studies with siRNA directed against FGFR1, or a small molecule inhibitor of the receptor, showed cell lines harbouring this amplification to be particularly sensitive to inhibition of this signalling pathway.
Alternatively, a biological sample taken from a patient may be analysed for loss of a negative regulator or suppressor of FGFR or VEGFR. In the present context, the term “loss” embraces the deletion of a gene encoding the regulator or suppressor, the truncation of the gene (for example by mutation), the truncation of the transcribed product of the gene, or the inactivation of the transcribed product (e.g. by point mutation) or sequestration by another gene product.
The term up-regulation includes elevated expression or over-expression, including gene amplification (i.e. multiple gene copies) and increased expression by a transcriptional effect, and hyperactivity and activation, including activation by mutations. Thus, the patient may be subjected to a diagnostic test to detect a marker characteristic of up-regulation of FGFR, and/or VEGFR. The term diagnosis includes screening. By marker we include genetic markers including, for example, the measurement of DNA composition to identify mutations of FGFR, and/or VEGFR. The term marker also includes markers which are characteristic of up regulation of FGFR and/or VEGFR, including enzyme activity, enzyme levels, enzyme state (e.g. phosphorylated or not) and mRNA levels of the aforementioned proteins.
The diagnostic tests and screens are typically conducted on a biological sample selected from tumour biopsy samples, blood samples (isolation and enrichment of shed tumour cells), stool biopsies, sputum, chromosome analysis, pleural fluid, peritoneal fluid, buccal spears, biopsy or urine.
Methods of identification and analysis of mutations and up-regulation of proteins are known to a person skilled in the art. Screening methods could include, but are not limited to, standard methods such as reverse-transcriptase polymerase chain reaction (RT-PCR) or in-situ hybridization such as fluorescence in situ hybridization (FISH).
Identification of an individual carrying a mutation in FGFR, and/or VEGFR may mean that the patient would be particularly suitable for treatment with a FGFR, and/or VEGFR inhibitor. Tumours may preferentially be screened for presence of a FGFR, and/or VEGFR variant prior to treatment. The screening process will typically involve direct sequencing, oligonucleotide microarray analysis, or a mutant specific antibody. In addition, diagnosis of tumours with such mutations could be performed using techniques known to a person skilled in the art and as described herein such as RT-PCR and FISH.
In addition, mutant forms of, for example FGFR or VEGFR2, can be identified by direct sequencing of, for example, tumour biopsies using PCR and methods to sequence PCR products directly as hereinbefore described. The skilled artisan will recognize that all such well-known techniques for detection of the over expression, activation or mutations of the aforementioned proteins could be applicable in the present case.
In screening by RT-PCR, the level of mRNA in the tumour is assessed by creating a cDNA copy of the mRNA followed by amplification of the cDNA by PCR. Methods of PCR amplification, the selection of primers, and conditions for amplification, are known to a person skilled in the art. Nucleic acid manipulations and PCR are carried out by standard methods, as described for example in Ausubel, F. M. et al., eds. (2004) Current Protocols in Molecular Biology, John Wiley & Sons Inc., or Innis, M. A. et al., eds. (1990) PCR Protocols: a guide to methods and applications, Academic Press, San Diego. Reactions and manipulations involving nucleic acid techniques are also described in Sambrook et al., (2001), 3rd Ed, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press. Alternatively a commercially available kit for RT-PCR (for example Roche Molecular Biochemicals) may be used, or methodology as set forth in U.S. Pat. Nos. 4,666,828; 4,683,202; 4,801,531; 5,192,659, 5,272,057, 5,882,864, and 6,218,529 and incorporated herein by reference. An example of an in-situ hybridisation technique for assessing mRNA expression would be fluorescence in-situ hybridisation (FISH) (see Angerer (1987) Meth. Enzymol., 152: 649).
Generally, in situ hybridization comprises the following major steps: (1) fixation of tissue to be analyzed; (2) prehybridization treatment of the sample to increase accessibility of target nucleic acid, and to reduce nonspecific binding; (3) hybridization of the mixture of nucleic acids to the nucleic acid in the biological structure or tissue; (4) post-hybridization washes to remove nucleic acid fragments not bound in the hybridization, and (5) detection of the hybridized nucleic acid fragments. The probes used in such applications are typically labelled, for example, with radioisotopes or fluorescent reporters. Preferred probes are sufficiently long, for example, from about 50, 100, or 200 nucleotides to about 1000 or more nucleotides, to enable specific hybridization with the target nucleic acid(s) under stringent conditions. Standard methods for carrying out FISH are described in Ausubel, F. M. et al., eds. (2004) Current Protocols in Molecular Biology, John Wiley & Sons Inc and Fluorescence In Situ Hybridization: Technical Overview by John M. S. Bartlett in Molecular Diagnosis of Cancer, Methods and Protocols, 2nd ed.; ISBN: 1-59259-760-2; March 2004, pps. 077-088; Series: Methods in Molecular Medicine.
Methods for gene expression profiling are described by (DePrimo et al. (2003), BMC Cancer, 3:3). Briefly, the protocol is as follows: double-stranded cDNA is synthesized from total RNA Using a (dT)24 oligomer for priming first-strand cDNA synthesis, followed by second strand cDNA synthesis with random hexamer primers. The double-stranded cDNA is used as a template for in vitro transcription of cRNA using biotinylated ribonucleotides. cRNA is chemically fragmented according to protocols described by Affymetrix (Santa Clara, Calif., USA), and then hybridized overnight on Human Genome Arrays.
Alternatively, the protein products expressed from the mRNAs may be assayed by immunohistochemistry of tumour samples, solid phase immunoassay with microtitre plates, Western blotting, 2-dimensional SDS-polyacrylamide gel electrophoresis, ELISA, flow cytometry and other methods known in the art for detection of specific proteins. Detection methods would include the use of site specific antibodies. The skilled person will recognize that all such well-known techniques for detection of upregulation of FGFR, and/or VEGFR, or detection of FGFR, and/or VEGFR variants or mutants could be applicable in the present case.
Abnormal levels of proteins such as FGFR or VEGFR can be measured using standard enzyme assays, for example, those assays described herein. Activation or overexpression could also be detected in a tissue sample, for example, a tumour tissue. By measuring the tyrosine kinase activity with an assay such as that from Chemicon International. The tyrosine kinase of interest would be immunoprecipitated from the sample lysate and its activity measured.
Alternative methods for the measurement of the over expression or activation of FGFR or VEGFR including the isoforms thereof, include the measurement of microvessel density. This can for example be measured using methods described by Orre and Rogers (Int J Cancer (1999), 84(2) 101-8). Assay methods also include the use of markers, for example, in the case of VEGFR these include CD31, CD34 and CD105.
Therefore all of these techniques could also be used to identify tumours particularly suitable for treatment with the compounds of the invention.
The compounds of the invention are particular useful in treatment of a patient having a mutated FGFR. The G697C mutation in FGFR3 is observed in 62% of oral squamous cell carcinomas and causes constitutive activation of the kinase activity. Activating mutations of FGFR3 have also been identified in bladder carcinoma cases. These mutations were of 6 kinds with varying degrees of prevalence: R248C, S249C, G372C, S373C, Y375C, K652Q. In addition, a Gly388Arg polymorphism in FGFR4 has been found to be associated with increased incidence and aggressiveness of prostate, colon, lung, liver (HCC) and breast cancer. The compounds of the invention are particular useful in treatment of a patient having a FGFR3-TACC3 translocation.
Therefore in a further aspect the invention includes use of a compound according to the invention for the manufacture of a medicament for the treatment or prophylaxis of a disease state or condition in a patient who has been screened and has been determined as suffering from, or being at risk of suffering from, a disease or condition which would be susceptible to treatment with a compound having activity against FGFR.
Particular mutations a patient is screened for include G697C, R248C, S249C, G372C, S373C, Y375C, K652Q mutations in FGFR3 and Gly388Arg polymorphism in FGFR4.
In another aspect the invention includes a compound of the invention for use in the prophylaxis or treatment of cancer in a patient selected from a sub-population possessing a variant of the FGFR gene (for example G697C mutation in FGFR3 and Gly388Arg polymorphism in FGFR4).
MRI determination of vessel normalization (e.g. using MRI gradient echo, spin echo, and contrast enhancement to measure blood volume, relative vessel size, and vascular permeability) in combination with circulating biomarkers (circulating progenitor cells (CPCs), CECs, SDF1, and FGF2) may also be used to identify VEGFR2-resistant tumours for treatment with a compound of the invention.
Pharmaceutical Compositions and Combinations
In view of their useful pharmacological properties, the subject compounds may be formulated into various pharmaceutical forms for administration purposes.
In one embodiment the pharmaceutical composition (e.g. formulation) comprises at least one active compound of the invention together with one or more pharmaceutically acceptable carriers, adjuvants, excipients, diluents, fillers, buffers, stabilisers, preservatives, lubricants, or other materials well known to those skilled in the art and optionally other therapeutic or prophylactic agents.
To prepare the pharmaceutical compositions of this invention, an effective amount of a compound of the present invention, as the active ingredient is combined in intimate admixture with a pharmaceutically acceptable carrier, which carrier may take a wide variety of forms depending on the form of preparation desired for administration. The pharmaceutical compositions can be in any form suitable for oral, parenteral, topical, intranasal, ophthalmic, otic, rectal, intra-vaginal, or transdermal administration. These pharmaceutical compositions are desirably in unitary dosage form suitable, preferably, for administration orally, rectally, percutaneously, or by parenteral injection. For example, in preparing the compositions in oral dosage form, any of the usual pharmaceutical media may be employed, such as, for example, water, glycols, oils, alcohols and the like in the case of oral liquid preparations such as suspensions, syrups, elixirs and solutions; or solid carriers such as starches, sugars, kaolin, lubricants, binders, disintegrating agents and the like in the case of powders, pills, capsules and tablets.
Because of their ease in administration, tablets and capsules represent the most advantageous oral dosage unit form, in which case solid pharmaceutical carriers are obviously employed. For parenteral compositions, the carrier will usually comprise sterile water, at least in large part, though other ingredients, to aid solubility for example, may be included. Injectable solutions, for example, may be prepared in which the carrier comprises saline solution, glucose solution or a mixture of saline and glucose solution. Injectable suspensions may also be prepared in which case appropriate liquid carriers, suspending agents and the like may be employed. In the compositions suitable for percutaneous administration, the carrier optionally comprises a penetration enhancing agent and/or a suitable wetting agent, optionally combined with suitable additives of any nature in minor proportions, which additives do not cause a significant deleterious effect to the skin. Said additives may facilitate the administration to the skin and/or may be helpful for preparing the desired compositions. These compositions may be administered in various ways, e.g., as a transdermal patch, as a spot-on, as an ointment. It is especially advantageous to formulate the aforementioned pharmaceutical compositions in dosage unit form for ease of administration and uniformity of dosage. Dosage unit form as used in the specification and claims herein refers to physically discrete units suitable as unitary dosages, each unit containing a predetermined quantity of active ingredient calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier. Examples of such dosage unit forms are tablets (including scored or coated tablets), capsules, pills, powder packets, wafers, injectable solutions or suspensions, teaspoonfuls, tablespoonfuls and the like, and segregated multiples thereof.
It is especially advantageous to formulate the aforementioned pharmaceutical compositions in dosage unit form for ease of administration and uniformity of dosage. Dosage unit form as used in the specification and claims herein refers to physically discrete units suitable as unitary dosages, each unit containing a predetermined quantity of active ingredient, calculated to produce the desired therapeutic effect, in association with the required pharmaceutical carrier. Examples of such dosage unit forms are tablets (including scored or coated tablets), capsules, pills, powder packets, wafers, injectable solutions or suspensions, teaspoonfuls, tablespoonfuls and the like, and segregated multiples thereof.
The compound of the invention is administered in an amount sufficient to exert its anti-tumour activity.
Those skilled in the art could easily determine the effective amount from the test results presented hereinafter. In general it is contemplated that a therapeutically effective amount would be from 0.005 mg/kg to 100 mg/kg body weight, and in particular from 0.005 mg/kg to 10 mg/kg body weight. It may be appropriate to administer the required dose as single, two, three, four or more sub-doses at appropriate intervals throughout the day. Said sub-doses may be formulated as unit dosage forms, for example, containing 0.5 to 500 mg, in particular 1 mg to 500 mg, more in particular 10 mg to 500 mg of active ingredient per unit dosage form.
Depending on the mode of administration, the pharmaceutical composition will preferably comprise from 0.05 to 99% by weight, more preferably from 0.1 to 70% by weight, even more preferably from 0.1 to 50% by weight of the compound of the present invention, and, from 1 to 99.95% by weight, more preferably from 30 to 99.9% by weight, even more preferably from 50 to 99.9% by weight of a pharmaceutically acceptable carrier, all percentages being based on the total weight of the composition.
As another aspect of the present invention, a combination of a compound of the present invention with another anticancer agent is envisaged, especially for use as a medicine, more specifically for use in the treatment of cancer or related diseases.
For the treatment of the above conditions, the compounds of the invention may be advantageously employed in combination with one or more other medicinal agents, more particularly, with other anti-cancer agents or adjuvants in cancer therapy. Examples of anti-cancer agents or adjuvants (supporting agents in the therapy) include but are not limited to:

    
    
        platinum coordination compounds for example cisplatin optionally combined with amifostine, carboplatin or oxaliplatin;
        taxane compounds for example paclitaxel, paclitaxel protein bound particles (AbraxaneTM) or docetaxel;
        topoisomerase I inhibitors such as camptothecin compounds for example irinotecan, SN-38, topotecan, topotecan hcl;
        topoisomerase II inhibitors such as anti-tumour epipodophyllotoxins or podophyllotoxin derivatives for example etoposide, etoposide phosphate or teniposide;
        anti-tumour vinca alkaloids for example vinblastine, vincristine or vinorelbine;
        anti-tumour nucleoside derivatives for example 5-fluorouracil, leucovorin, gemcitabine, gemcitabine hcl, capecitabine, cladribine, fludarabine, nelarabine;
        alkylating agents such as nitrogen mustard or nitrosourea for example cyclophosphamide, chlorambucil, carmustine, thiotepa, mephalan (melphalan), lomustine, altretamine, busulfan, dacarbazine, estramustine, ifosfamide optionally in combination with mesna, pipobroman, procarbazine, streptozocin, telozolomide, uracil;
        anti-tumour anthracycline derivatives for example daunorubicin, doxorubicin optionally in combination with dexrazoxane, doxil, idarubicin, mitoxantrone, epirubicin, epirubicin hcl, valrubicin;
        molecules that target the IGF-1 receptor for example picropodophilin;
        tetracarcin derivatives for example tetrocarcin A;
        glucocorticoids for example prednisone;
        antibodies for example trastuzumab (HER2 antibody), rituximab (CD20 antibody), gemtuzumab, gemtuzumab ozogamicin, cetuximab, pertuzumab, bevacizumab, alemtuzumab, eculizumab, ibritumomab tiuxetan, nofetumomab, panitumumab, tositumomab, CNTO 328;
        estrogen receptor antagonists or selective estrogen receptor modulators or inhibitors of estrogen synthesis for example tamoxifen, fulvestrant, toremifene, droloxifene, faslodex, raloxifene or letrozole;
        aromatase inhibitors such as exemestane, anastrozole, letrazole, testolactone and vorozole;
        differentiating agents such as retinoids, vitamin D or retinoic acid and retinoic acid metabolism blocking agents (RAMBA) for example accutane;
        DNA methyl transferase inhibitors for example azacytidine or decitabine;
        antifolates for example premetrexed disodium;
        antibiotics for example antinomycin D, bleomycin, mitomycin C, dactinomycin, carminomycin, daunomycin, levamisole, plicamycin, mithramycin;
        antimetabolites for example clofarabine, aminopterin, cytosine arabinoside or methotrexate, azacitidine, cytarabine, floxuridine, pentostatin, thioguanine; apoptosis inducing agents and antiangiogenic agents such as Bcl-2 inhibitors for example YC 137, BH 312, ABT 737, gossypol, HA 14-1, TW 37 or decanoic acid;
        tubuline-binding agents for example combrestatin, colchicines or nocodazole;
        kinase inhibitors (e.g. EGFR (epithelial growth factor receptor) inhibitors, MTKI (multi target kinase inhibitors), mTOR inhibitors, cmet inhibitors) for example flavoperidol, imatinib mesylate, erlotinib, gefitinib, dasatinib, lapatinib, lapatinib ditosylate, sorafenib, sunitinib, sunitinib maleate, temsirolimus, 6-{difluoro[6-(1-methyl-1H-pyrazol-4-yl)[1,2,4]triazolo[4,3-b]pyridazin-3-yl]methyl}quinoline or a pharmaceutically acceptable salt thereof, 6-[difluoro(6-pyridin-4-yl[1,2,4]triazolo[4,3-b]pyridazin-3-yl)methyl]quinoline or a pharmaceutically acceptable salt thereof;
        farnesyltransferase inhibitors for example tipifarnib;
        histone deacetylase (HDAC) inhibitors for example sodium butyrate, suberoylanilide hydroxamide acid (SAHA), depsipeptide (FR 901228), NVP-LAQ824, R306465, JNJ-26481585, trichostatin A, vorinostat;
        Inhibitors of the ubiquitin-proteasome pathway for example PS-341, MLN 0.41 or bortezomib;
        Yondelis;
        Telomerase inhibitors for example telomestatin;
        Matrix metalloproteinase inhibitors for example batimastat, marimastat, prinostat or metastat.
        Recombinant interleukins for example aldesleukin, denileukin diftitox, interferon alfa 2a, interferon alfa 2b, peginterferon alfa 2b
        MAPK inhibitors
        Retinoids for example alitretinoin, bexarotene, tretinoin
        Arsenic trioxide
        Asparaginase
        Steroids for example dromostanolone propionate, megestrol acetate, nandrolone (decanoate, phenpropionate), dexamethasone
        Gonadotropin releasing hormone agonists or antagonists for example abarelix, goserelin acetate, histrelin acetate, leuprolide acetate
        Thalidomide, lenalidomide
        Mercaptopurine, mitotane, pamidronate, pegademase, pegaspargase, rasburicase
        BH3 mimetics for example ABT-737
        MEK inhibitors for example PD98059, AZD6244, CI-1040
        colony-stimulating factor analogs for example filgrastim, pegfilgrastim, sargramostim; erythropoietin or analogues thereof (e.g. darbepoetin alfa); interleukin 11; oprelvekin; zoledronate, zoledronic acid; fentanyl; bisphosphonate; palifermin.
        a steroidal cytochrome P450 17alpha-hydroxylase-17,20-lyase inhibitor (CYP17), e.g. abiraterone, abiraterone acetate.
    
    


In one embodiment, the present invention relates to a combination of a compound of formula (I), a pharmaceutically acceptable salt thereof or a solvate thereof, or any sub-groups and examples thereof, and 6-{difluoro[6-(1-methyl-1H-pyrazol-4-yl)[1,2,4]triazolo[4,3-b]pyridazin-3-yl]methyl}quinoline or a pharmaceutically acceptable salt thereof.
In one embodiment, the present invention relates to a combination of a compound of formula (I), a pharmaceutically acceptable salt thereof or a solvate thereof, or any sub-groups and examples thereof, and 6-[difluoro(6-pyridin-4-yl[1,2,4]triazolo[4,3-b]pyridazin-3-yl)methyl]quinoline or a pharmaceutically acceptable salt thereof.
In one embodiment, the present invention relates to a pharmaceutical composition comprising a compound of formula (I), a pharmaceutically acceptable salt thereof or a solvate thereof, or any sub-groups and examples thereof, and 6-{difluoro[6-(1-methyl-1H-pyrazol-4-yl)[1,2,4]triazolo[4,3-b]pyridazin-3-yl]methyl}quinoline or a pharmaceutically acceptable salt thereof.
In one embodiment, the present invention relates to a pharmaceutical composition comprising a compound of formula (I), a pharmaceutically acceptable salt thereof or a solvate thereof, or any sub-groups and examples thereof, and 6-[difluoro(6-pyridin-4-yl[1,2,4]triazolo[4,3-b]pyridazin-3-yl)methyl]quinoline or a pharmaceutically acceptable salt thereof.
The compounds of the present invention also have therapeutic applications in sensitising tumour cells for radiotherapy and chemotherapy.
Hence the compounds of the present invention can be used as “radiosensitizer” and/or “chemosensitizer” or can be given in combination with another “radiosensitizer” and/or “chemosensitizer”.
The term “radiosensitizer”, as used herein, is defined as a molecule, preferably a low molecular weight molecule, administered to animals in therapeutically effective amounts to increase the sensitivity of the cells to ionizing radiation and/or to promote the treatment of diseases which are treatable with ionizing radiation.
The term “chemosensitizer”, as used herein, is defined as a molecule, preferably a low molecular weight molecule, administered to animals in therapeutically effective amounts to increase the sensitivity of cells to chemotherapy and/or promote the treatment of diseases which are treatable with chemotherapeutics.
Several mechanisms for the mode of action of radiosensitizers have been suggested in the literature including: hypoxic cell radio sensitizers (e.g., 2-nitroimidazole compounds, and benzotriazine dioxide compounds) mimicking oxygen or alternatively behave like bioreductive agents under hypoxia; non-hypoxic cell radiosensitizers (e.g., halogenated pyrimidines) can be analogoues of DNA bases and preferentially incorporate into the DNA of cancer cells and thereby promote the radiation-induced breaking of DNA molecules and/or prevent the normal DNA repair mechanisms; and various other potential mechanisms of action have been hypothesized for radiosensitizers in the treatment of disease.
Many cancer treatment protocols currently employ radiosensitizers in conjunction with radiation of x-rays. Examples of x-ray activated radiosensitizers include, but are not limited to, the following: metronidazole, misonidazole, desmethylmisonidazole, pimonidazole, etanidazole, nimorazole, mitomycin C, RSU 1069, SR 4233, EO9,
RB 6145, nicotinamide, 5-bromodeoxyuridine (BUdR), 5-iododeoxyuridine (IUdR), bromodeoxycytidine, fluorodeoxyuridine (FudR), hydroxyurea, cisplatin, and therapeutically effective analogs and derivatives of the same.
Photodynamic therapy (PDT) of cancers employs visible light as the radiation activator of the sensitizing agent. Examples of photodynamic radiosensitizers include the following, but are not limited to: hematoporphyrin derivatives, Photofrin, benzoporphyrin derivatives, tin etioporphyrin, pheoborbide-a, bacteriochlorophyll-a, naphthalocyanines, phthalocyanines, zinc phthalocyanine, and therapeutically effective analogs and derivatives of the same. Radiosensitizers may be administered in conjunction with a therapeutically effective amount of one or more other compounds, including but not limited to: compounds which promote the incorporation of radiosensitizers to the target cells; compounds which control the flow of therapeutics, nutrients, and/or oxygen to the target cells; chemotherapeutic agents which act on the tumour with or without additional radiation; or other therapeutically effective compounds for treating cancer or other diseases.
Chemosensitizers may be administered in conjunction with a therapeutically effective amount of one or more other compounds, including but not limited to: compounds which promote the incorporation of chemosensitizers to the target cells; compounds which control the flow of therapeutics, nutrients, and/or oxygen to the target cells; chemotherapeutic agents which act on the tumour or other therapeutically effective compounds for treating cancer or other disease. Calcium antagonists, for example verapamil, are found useful in combination with antineoplastic agents to establish chemosensitivity in tumor cells resistant to accepted chemotherapeutic agents and to potentiate the efficacy of such compounds in drug-sensitive malignancies.
In view of their useful pharmacological properties, the components of the combinations according to the invention, i.e. the one or more other medicinal agent and the compound according to the present invention may be formulated into various pharmaceutical forms for administration purposes. The components may be formulated separately in individual pharmaceutical compositions or in a unitary pharmaceutical composition containing all components.
The present invention therefore also relates to a pharmaceutical composition comprising the one or more other medicinal agent and the compound according to the present invention together with a pharmaceutical carrier.
The present invention further relates to the use of a combination according to the invention in the manufacture of a pharmaceutical composition for inhibiting the growth of tumour cells.
The present invention further relates to a product containing as first active ingredient a compound according to the invention and as further active ingredient one or more anticancer agent, as a combined preparation for simultaneous, separate or sequential use in the treatment of patients suffering from cancer.
The one or more other medicinal agents and the compound according to the present invention may be administered simultaneously (e.g. in separate or unitary compositions) or sequentially in either order. In the latter case, the two or more compounds will be administered within a period and in an amount and manner that is sufficient to ensure that an advantageous or synergistic effect is achieved. It will be appreciated that the preferred method and order of administration and the respective dosage amounts and regimes for each component of the combination will depend on the particular other medicinal agent and compound of the present invention being administered, their route of administration, the particular tumour being treated and the particular host being treated. The optimum method and order of administration and the dosage amounts and regime can be readily determined by those skilled in the art using conventional methods and in view of the information set out herein.
The weight ratio of the compound according to the present invention and the one or more other anticancer agent(s) when given as a combination may be determined by the person skilled in the art. Said ratio and the exact dosage and frequency of administration depends on the particular compound according to the invention and the other anticancer agent(s) used, the particular condition being treated, the severity of the condition being treated, the age, weight, gender, diet, time of administration and general physical condition of the particular patient, the mode of administration as well as other medication the individual may be taking, as is well known to those skilled in the art. Furthermore, it is evident that the effective daily amount may be lowered or increased depending on the response of the treated subject and/or depending on the evaluation of the physician prescribing the compounds of the instant invention. A particular weight ratio for the present compound of formula (I) and another anticancer agent may range from 1/10 to 10/1, more in particular from 1/5 to 5/1, even more in particular from 1/3 to 3/1.
The platinum coordination compound is advantageously administered in a dosage of 1 to 500 mg per square meter (mg/m2) of body surface area, for example 50 to 400 mg/m2, particularly for cisplatin in a dosage of about 75 mg/m2 and for carboplatin in about 300 mg/m2 per course of treatment.
The taxane compound is advantageously administered in a dosage of 50 to 400 mg per square meter (mg/m2) of body surface area, for example 75 to 250 mg/m2, particularly for paclitaxel in a dosage of about 175 to 250 mg/m2 and for docetaxel in about 75 to 150 mg/m2 per course of treatment.
The camptothecin compound is advantageously administered in a dosage of 0.1 to 400 mg per square meter (mg/m2) of body surface area, for example 1 to 300 mg/m2, particularly for irinotecan in a dosage of about 100 to 350 mg/m2 and for topotecan in about 1 to 2 mg/m2 per course of treatment.
The anti-tumour podophyllotoxin derivative is advantageously administered in a dosage of 30 to 300 mg per square meter (mg/m2) of body surface area, for example 50 to 250 mg/m2, particularly for etoposide in a dosage of about 35 to 100 mg/m2 and for teniposide in about 50 to 250 mg/m2 per course of treatment.
The anti-tumour vinca alkaloid is advantageously administered in a dosage of 2 to 30 mg per square meter (mg/m2) of body surface area, particularly for vinblastine in a dosage of about 3 to 12 mg/m2, for vincristine in a dosage of about 1 to 2 mg/m2, and for vinorelbine in dosage of about 10 to 30 mg/m2 per course of treatment.
The anti-tumour nucleoside derivative is advantageously administered in a dosage of 200 to 2500 mg per square meter (mg/m2) of body surface area, for example 700 to 1500 mg/m2, particularly for 5-FU in a dosage of 200 to 500 mg/m2, for gemcitabine in a dosage of about 800 to 1200 mg/m2 and for capecitabine in about 1000 to 2500 mg/m2 per course of treatment.
The alkylating agents such as nitrogen mustard or nitrosourea is advantageously administered in a dosage of 100 to 500 mg per square meter (mg/m2) of body surface area, for example 120 to 200 mg/m2, particularly for cyclophosphamide in a dosage of about 100 to 500 mg/m2, for chlorambucil in a dosage of about 0.1 to 0.2 mg/kg, for carmustine in a dosage of about 150 to 200 mg/m2, and for lomustine in a dosage of about 100 to 150 mg/m2 per course of treatment.
The anti-tumour anthracycline derivative is advantageously administered in a dosage of 10 to 75 mg per square meter (mg/m2) of body surface area, for example 15 to 60 mg/m2, particularly for doxorubicin in a dosage of about 40 to 75 mg/m2, for daunorubicin in a dosage of about 25 to 45 mg/m2, and for idarubicin in a dosage of about 10 to 15 mg/m2 per course of treatment.
The antiestrogen agent is advantageously administered in a dosage of about 1 to 100 mg daily depending on the particular agent and the condition being treated. Tamoxifen is advantageously administered orally in a dosage of 5 to 50 mg, preferably 10 to 20 mg twice a day, continuing the therapy for sufficient time to achieve and maintain a therapeutic effect. Toremifene is advantageously administered orally in a dosage of about 60 mg once a day, continuing the therapy for sufficient time to achieve and maintain a therapeutic effect. Anastrozole is advantageously administered orally in a dosage of about 1 mg once a day. Droloxifene is advantageously administered orally in a dosage of about 20-100 mg once a day. Raloxifene is advantageously administered orally in a dosage of about 60 mg once a day. Exemestane is advantageously administered orally in a dosage of about 25 mg once a day.
Antibodies are advantageously administered in a dosage of about 1 to 5 mg per square meter (mg/m2) of body surface area, or as known in the art, if different. Trastuzumab is advantageously administered in a dosage of 1 to 5 mg per square meter (mg/m2) of body surface area, particularly 2 to 4 mg/m2 per course of treatment.
These dosages may be administered for example once, twice or more per course of treatment, which may be repeated for example every 7, 14, 21 or 28 days.
The compounds of formula (I), the pharmaceutically acceptable addition salts, in particular pharmaceutically acceptable acid addition salts, and stereoisomeric forms thereof can have valuable diagnostic properties in that they can be used for detecting or identifying the formation of a complex between a labelled compound and other molecules, peptides, proteins, enzymes or receptors.
The detecting or identifying methods can use compounds that are labelled with labelling agents such as radioisotopes, enzymes, fluorescent substances, luminous substances, etc. Examples of the radioisotopes include 125I, 131I, 3H and 14C. Enzymes are usually made detectable by conjugation of an appropriate substrate which, in turn catalyses a detectable reaction. Examples thereof include, for example, beta-galactosidase, beta-glucosidase, alkaline phosphatase, peroxidase and malate dehydrogenase, preferably horseradish peroxidase. The luminous substances include, for example, luminol, luminol derivatives, luciferin, aequorin and luciferase. Biological samples can be defined as body tissue or body fluids. Examples of body fluids are cerebrospinal fluid, blood, plasma, serum, urine, sputum, saliva and the like.
General Synthetic Routes
The following examples illustrate the present invention but are examples only and are not intended to limit the scope of the claims in any way.
Experimental Part
Hereinafter, the term ‘DCM’ means dichloromethane, ‘Me’ means methyl, ‘Et’ means ethyl, ‘MeOH’ means methanol, ‘DMF’ means dimethylformamide, ‘Et2O’ means diethyl ether, ‘EtOAc’ means ethyl acetate, ‘ACN’ means acetonitrile, ‘H2O’ means water, ‘THF’ means tetrahydrofuran, ‘MgSO4’ means magnesium sulfate, ‘NH4OH’ means ammoniumhydroxide, ‘K2CO3’ means dipotassium carbonate, ‘MgCl2’ means magnesium chloride, ‘iPrNH2’ means isopropylamine, ‘NH4HCO3’ means ammonium bicarbonate, ‘DMSO’ means dimethyl sulfoxide, ‘EDTA’ means ethylenediaminetetraacetic acid, ‘NADP’ means nicotinamide adenine dinucleotide phosphate, ‘SFC’ means supercritical fluid chromatography, ‘MP’ means melting point.
A. Preparation of the Intermediates
Intermediate 1 or N-(3,5-dimethoxyphenyl)-N′-(1-methylethyl)-N-[3-(1-methyl-1H-pyrazol-4-yl)quinoxalin-6-yl]ethane-1,2-diamine is described as compound 4 in WO2011/135376 and can be prepared according to the protocols described therein for compound 4.
Intermediate 2 or N-(3,5-dimethoxyphenyl)-N′-(1-methylethyl)-N-[3-(1H-pyrazol-4-yl)quinoxalin-6-yl]ethane-1,2-diamine is described as free base compound 137 in WO2011/135376 and can be prepared according to the protocols described therein for compound 137.
Intermediate 3 or N-(3,5-dimethoxyphenyl)-N′-(1-methyl)-N-[3-(1-ethyl-1H-pyrazol-4-yl)quinoxalin-6-yl]ethane-1,2-diamine is described as compound 449 in WO2011/135376 and can be prepared according to the protocols described therein for compound 449.
Intermediate 4 or N-(3,5-dimethoxyphenyl)-N′-(1-methylethyl)-N-[3-(1-ethyl-1H-pyrazol-4-yl)quinoxalin-6-yl]ethane-1,2-diamine is described as free base or HCl salt as compound 135 in WO2011/135376 and can be prepared according to the protocols described therein for compound 135.
Intermediate 5 or N-(3,5-dimethoxyphenyl)-N′-(1-methylethyl)-N-[3-(1-methyl-1H-pyrazol-4-yl)quinoxalin-6-yl]propane-1,3-diamine is described as compound 382 in WO2011/135376 and can be prepared according to the protocols described therein for compound 382.
Intermediate 6 or 7-bromo-2-(1-methyl-1H-pyrazol-4-yl)-quinoxaline is described as intermediate 2 in WO2011/135376 and can be prepared according to the protocols described therein for intermediate 2.
WO2011/135376 is incorporated herein by reference.
Example A1
a) Preparation of Intermediate 7




A mixture of intermediate 6 (5 g; 17 mmol), 2-fluoro-3,5-dimethoxyaniline (3.6 g; 21 mmol), sodium tert-butoxide (5 g; 52 mmol) and rac-bis(diphenylphosphino)-1,1′-binaphthyl (0.54 g; 0.87 mmol) in dioxane (100 mL) was degassed at room temperature under nitrogen flow. After 10 minutes, palladium (II) acetate (388 mg; 1.7 mmol) was added portionwise at room temperature under nitrogen flow. The reaction mixture was heated at 95° C. for 5 hours. The reaction mixture was cooled to room temperature and poured onto iced water and DCM. The mixture was filtered through a pad of Celite®. The organic layer was separated, dried over MgSO4, filtered and evaporated to dryness. The residue was crystallized from diethylether and the precipitate was filtered off, dried under vacuum to give 4 g (61%) of intermediate 7.
b) Preparation of Intermediate 8




Sodium hydride (0.21 g; 5.35 mmol) was added to a solution of intermediate 7 (0.7 g; 1.85 mmol) in DMF (25 mL) at 5° C. under nitrogen flow. The mixture was stirred at 5° C. for 1 hour. (2-Bromoethoxy)-tert-butyldimethylsilane (0.51 mL; 2.40 mmol) was added dropwise at 5° C. under nitrogen flow and the reaction mixture was stirred at room temperature for 24 hours. The mixture was poured into cooled water and the product was extracted with EtOAc. The organic layer was washed with H2O, dried over MgSO4, filtered and evaporated to give 1.2 g (quant.) of intermediate 8. The crude product was used without any purification in the next step.
c) Preparation of Intermediate 9




Tetrabutyl ammonium fluoride (1M in THF) (2 mL; 2 mmol) was added to a solution of intermediate 8 (1 g; 1.85 mmol) in THF (20 mL) and the reaction mixture was stirred for 3 hours at room temperature. The reaction mixture was partitioned between water and EtOAc. The organic layer was washed with brine, dried over MgSO4, filtered and evaporated to dryness. The residue (1.2 g) was purified by chromatography over silica gel (irregular SiOH, 15-40 μm; 80 g; eluent: 98% DCM, 2% MeOH, 0.1% NH4OH). The pure fractions were collected and the solvent was evaporated. The residue (500 mg) was crystallized from diethylether. The precipitate was filtered and dried to give 410 mg (52%) of intermediate 9. MP: 172° C. (K).
d) Preparation of Intermediate 10




Methanesulfonyl chloride (0.3 mL; 3.88 mmol) was added dropwise at 5° C. to a solution of intermediate 9 (547 mg; 1.29 mmol) and triethylamine (0.9 mL; 6.46 mmol) in DCM (15 mL). The reaction mixture was stirred at this temperature for 1 hour, diluted with DCM and poured onto 10% aqueous solution of K2CO3. The organic layer was decanted, dried over MgSO4, filtered and evaporated to give 850 mg (>100%) of intermediate 10. The crude product was used without purification in the next step.
e) Preparation of Intermediate 11




A mixture of intermediate 10 (0.648 g; 1.29 mmol) and isopropylamine (2.4 mL; 28 mmol) in CH3CN (15 mL) was heated at 100° C. for 24 hours in a sealed tube. The reaction mixture was cooled to room temperature, diluted with DCM and poured onto water. The organic layer was decanted, dried over MgSO4, filtered and evaporated to dryness. The residue was purified by chromatography over silica gel (irregular SiOH; 24 g; gradient: from 3% MeOH, 97% DCM to 10% MeOH, 90% DCM). The pure fractions were collected and evaporated to give 452 mg (75%) of intermediate 11.
B. Preparation of the Compounds of Formula (I)
Example B1
Preparation of Compound 1




A solution of N-(3,5-dimethoxyphenyl)-N′-(1-methylethyl)-N-[3-(1-methyl-1H-pyrazol-4-yl)quinoxalin-6-yl]ethane-1,2-diamine (intermediate 1) (0.42 g; 0.9 mmol), formaldehyde (37% solution in water; 0.21 mL; 2.8 mmol) in dioxane (8 mL) was stirred at room temperature for 3 days. Water and EtOAc were added. The organic layer was decanted, washed with water, dried over MgSO4 and evaporated to dryness. The residue (0.52 g) was purified by chromatography over silica gel (Stationary phase: Spherical bare silica 5 μm 150×30.0 mm, Mobile phase: Gradient from 0.2% NH4OH, 98% DCM, 2% MeOH to 1.2% NH4OH, 88% DCM, 12% MeOH). The fractions containing the desired product were collected and evaporated to dryness. The residue (0.37 g) was crystallized from a mixture of MeOH and Et2O. The precipitate was filtered off and dried, yielding 0.27 g (64%) of compound 1 (MP: 190° C. (DSC)).
Example B2
Preparation of Compound 2




A solution of N-(3,5-dimethoxyphenyl)-N′-(1-methylethyl)-N-[3-(1H-pyrazol-4-yl)quinoxalin-6-yl]ethane-1,2-diamine (intermediate 2) (0.24 g; 0.52 mmol), formaldehyde (37% solution in water; 0.12 mL; 1.55 mmol) in dioxane (8 mL) was stirred at room temperature for 3 days without transformation. K2CO3 (0.22 g; 1.55 mmol) was added and the solution was further stirred at room temperature for 3 days. The organic layer was extracted, dried over MgSO4 and evaporated to dryness. The residue (0.176 g) was purified by chromatography over silica gel (Stationary phase: Spherical bare silica 5 μm 150×30.0 mm, Mobile phase: Gradient from 0.2% NH4OH, 98% DCM, 2% MeOH to 1.3% NH4OH, 87% DCM, 13% MeOH). The fractions containing the desired product were collected and evaporated to dryness. The residue (79 mg) was freeze-dried with acetonitrile/water 20/80 to give 66 mg (34%) of compound 2 as a yellow gummy powder.
Example B3
Preparation of Compound 3 and 4
50 μM of intermediate 1 was incubated at 37° C. with the 12,000 g fraction from rat liver for 60 minutes at 1 mg/ml protein. A stock solution of 10 mM of intermediate 1 in methanol was prepared and this was diluted 200-fold (0.25 ml in 50 ml) in the incubation medium (final methanol concentration 0.5% in the incubate). The incubation buffer contained 1 mM EDTA, 5 mM MgCl2, and 100 mM potassium phosphate buffer (pH 7.4). Reaction was initiated by addition of NADP (1 mM final concentration). Incubation was stopped by flash freezing on dry ice.
The resulting metabolites were initially extracted using ethyl acetate. The metabolite fraction was evaporated to dryness, reconstituted in DMSO:water (1:1, v/v) and separated using reverse phase UPLC. Separation was achieved using two Interchim Strategy C18-2, 2.2 μm, (150 mm×3.0 mm ID) columns using solvent A with a linear gradient of 5-70% B over 20 minutes at 0.8 ml/min. Solvents consisted of Solvent A, 25 mM Ammonium acetate pH4.0 and Solvent B, Acetonitrile/methanol (60/40, v/v). Peak fractions corresponding to the desired products were collected, and evaporated to dryness, yielding compound 3 and 4.
Compound 3




Compound 3 can also be prepared according to the protocol described in Example 1 starting from compound 645 of WO2011/135376.
Compound 4




Alternatively, compound 3 and 4 were also prepared as follows:
Boron tribromide (1M in DCM; 6 mL; 6 mmol) was added dropwise to a solution of compound 1 (485 mg; 1.06 mmol) in DCM (25 mL) at 5° C. under nitrogen flow. The solution was allowed raise to room temperature slowly and was stirred for 1.5 hour. The reaction mixture was diluted with DCM, poured onto brine and basified with solid K2CO3. The organic layer was separated, washed with brine, dried over MgSO4, filtered and evaporated to dryness. The residue was purified by chromatography over silica gel (irregular SiOH, 40 g; mobile phase: gradient from 0.5% NH4OH, 94.5% DCM, 5% MeOH to 0.5% NH4OH, 89.5% DCM, 10% MeOH). The fractions containing the product were collected and evaporated to dryness yielding 110 mg (23%) of compound 1 and 287 mg of a mixture of compounds 3 and 4. This latter fraction was purified by achiral SFC (Chiralpak AD-H 5 μm 250*30 mn; mobile phase: 0.3% isopropylamine, 70% CO2, 30% MeOH). The pure fractions were collected, concentrated and crystallized from Et2O/ACN. The precipitates were filtered to afford 53 mg (11%) of compound 3 (MP: 255° C., K) and 148 mg (31%) of compound 4 (MP: 256° C., K).
Example B4
A stock solution of 2 mM of intermediate 1 was prepared in methanol and this was diluted 200-fold in the incubation medium (final methanol concentration 0.5% in the incubate). Incubation was done at 37° C. with the 12,000 g fraction from rat liver for 60 minutes at 1 mg/ml protein. The incubation buffer contained 1 mM EDTA, 5 mM MgCl2, and 100 mM potassium phosphate buffer (pH 7.4). Reaction was initiated by addition of NADP (1 mM final concentration). Incubation was stopped by flash freezing on dry ice.
The resulting incubation (1 ml) was mixed with 5 volumes of acetonitrile, vortex mixed and sonicated for 10 minutes. Protein was removed by centrifugation at 3200 rpm at 8° C. for 30 minutes. The supernatant was removed and evaporated to dryness under a stream of nitrogen at 30° C. The extract was reconstituted in acetonitrile/water (1:1, v/v). The sample was analysed as follows:
UPLC with MS Detection

    
    
        Ultra performance liquid chromatography Pump
        Acquity Binary Solvent Manager/Waters 2777 CTC-Pal-injector
        UV detector:
        Waters Acquity PDA
        MS Detector:
        Waters G2(S) QToF MS/Thermo LTQ-Orbitrap
        Data System:
        Waters Masslynx 4.1
    
    


Operating Conditions:

    
    
        Column:
        Interchim, Strategy C18-2, 2.2 μm 2×(150 mm×3.0 mm ID)
        Column temperature:
        T=60° C.
        Sample temperature:
        T=10° C.
        Mobile phase:
        Solvent A: 0.025 M ammonium acetate pH 4.0
        Solvent B: 60/40 (v/v) acetonitrile/methanol
        Elution mode:
        linear gradient:
    
    














Time (min)














0
5
22
22.5
25
25.5
















% A
95
80
50
0
0
95


% B
5
20
50
100
100
5









    
    
        Run Time: 30 min
        Flow: 0.8 ml/min
    
    


Syringe Infusion:

    
    
        Mobile phase: Acetonitrile:water (1:1, v/v)
        Flow: 5 μl/min
    
    


Detection Conditions
MS Conditions—Waters Synapt g2 and g2s Mass Spectrometer

    
    
        MS analysis was carried out using Waters SYNAPT G2 and G2S mass spectrometers, equipped with a dual electrospray ionisation probe and was operated in high resolution, positive ion mode. The capillary voltage was set at 3 kV and the cone at 40V. The source temperature was 120° C., desolvation temperature 400° C. The mass spectrometer was calibrated with a Sodium Formate solution delivered through the Sample Spray. The LockSprayTM ESI probe provided an independent source of the lock mass calibrant Leucine Enkephaline. The Leucine ion at m/z 556.2771 was used as lock mass in full MS as well as in MSMS-mode. The QTOF data (MS, MSMS) were acquired in the centroid mode with a variable scan time (0.5-1.0 sec). All data were processed using Masslynx software.
    
    


MS Conditions—Thermo Ltq-Orbitrap Mass Spectrometer

    
    
        The LTQ-Orbitrap mass spectrometer was equipped with an electrospray ionisation source operated in the positive ion mode. Accurate mass measurements were obtained using external calibration or a lock mass calibration (lock mass ion at m/z 391.2843). The source parameters were tuned for maximum sensitivity using a 10 ng/4 unchanged drug standard solution. The same solution was used to define the optimal collision energy employed during MSn fragmentation. Metabolites were selected for MSn fragmentation out of the LC-MS trace using data dependent scanning Data were acquired in the centroid mode and processed using XCalibur software.
    
    


In the above experiment compound 4 ([MH]+ m/z 445), compound 3 ([MH]+ m/z 445) and compound 5 ([MH]+ m/z 431) were detected.
Compound 5:




Example B5
Preparation of Compound 6




A solution of intermediate 3 (292 mg; 0.675 mmol), formaldehyde (37% solution in water; 151 μL; 2.02 mmol) in 1,4-dioxane (5.48 mL) was stirred at room temperature for 3 days. As low transformation was noticed, additional formaldehyde (37% solution in water; 252 μL; 3.37 mmol) was added and the reaction mixture was stirred at 70° C. for 16 hours.
H2O and EtOAc were added. The organic layer was decanted, dried over MgSO4, filtered and evaporated to dryness.
The residue (0.325 g) was purified by silica gel chromatography (irregular SiOH, 40 g, mobile phase: gradient from 95% DCM, 5% MeOH, 0.5% NH4OH to 90% DCM, 10% MeOH, 1% NH4OH). The fractions containing the product were mixed and concentrated to afford an intermediate fraction (106 mg) which was crystallized from a mixture of Et2O/ACN to afford after filtration and drying 86 mg (28%) of compound 6. MP: 170° C. (K)
Example B6
Preparation of Compound 7




as a HCl salt (1.65 HCl 2.2H2O)

A solution of intermediate 4 (293 mg; 0638 mmol), formaldehyde (37% solution in water; 143 μL; 1.91 mmol) in 1,4-dioxane (5.16 mL) was stirred at room temperature for 3 days. As no transformation was noticed, additional formaldehyde (37% solution in water; 238 μL; 3.18 mmol) was added and the reaction mixture was stirred at 70° C. for 16 hours. Again, additional formaldehyde (37% solution in water; 477 μL; 6.36 mmol) was added and the reaction mixture was stirred at 70° C. for 16 hours. H2O and EtOAc were added. The organic layer was decanted, dried over MgSO4, filtered and evaporated to dryness.
The residue (0.48 g) was purified by silica gel chromatography (Spherical bare silica 5 μm 150×30.0 mm, Mobile phase: Gradient from 0.2% NH4OH, 98% DCM, 2% MeOH to 1% NH4OH, 90% DCM, 10% MeOH). The fractions containing the product were mixed and concentrated to afford 148 mg of an intermediate fraction which was purified by achiral SFC (Stationary phase: CYANO 6 μm 150×21.2 mm, Mobile phase: 90% CO2, 10% MeOH (0.3% iPrNH2)). The fractions containing the product were mixed and concentrated to afford 100 mg of an intermediate fraction which was dissolved in MeOH. 0.1 mL of HCl in iPrOH (2-5N) was added at 0° C. The mixture was then concentrated and the resulting residue was taken up with Et2O. The precipitate was filtered and dried to afford 103 mg (29%) of compound 7 as a red solid. MP: 152° C. (K)
Example B7
Preparation of Compound 8




A solution of intermediate 11 (382 mg; 0.82 mmol) and formaldehyde (37% solution in water; 308 μL; 4.11 mmol) in dioxane (10 mL) was heated at 60° C. for 3 days. H2O and EtOAc were added. The organic layer was decanted, dried over MgSO4, filtered and evaporated to dryness. The residue was purified by chromatography over silica gel (Spherical bare silica 5 μm 150×30.0 mm; gradient: from 71% heptane, 1% MeOH (+10% NH4OH), 28% EtOAc to 0% heptane, 20% MeOH (+10% NH4OH), 80% EtOAc). The pure fractions were collected and evaporated to dryness. The residue (65 mg) was purified by reverse phase chromatography (X-Bridge-C18 5 μm 30*150 mm; gradient: from 80% NH4HCO3 0.5%, 20% CH3CN to 0% NH4HCO3 0.5%, 100% CH3CN). The pure fractions were collected and evaporated to give 15 mg (4%) of compound 8. MP: 266° C. (K).
Example B8
Preparation of Compound 9




A solution of intermediate 5 (0.21 g; 0.46 mmol) and formaldehyde (37% solution in water; 0.1 mL; 1.4 mmol) in 1,4-dioxane (8 mL) was stirred at room temperature for 3 days. After a week, additional formaldehyde (37% solution in water; 0.5 mL; 20.55 mmol) was added, the mixture was stirred at room temperature for further 2 days. H2O and EtOAc were added. The organic layer was extracted, dried over MgSO4 and evaporated to dryness.
The resulting residue (170 mg) was purified by reverse phase (Stationary phase: X-Bridge-C18 5 μm 30*150 mm, Mobile phase: Gradient from 85% NH4HCO3 0.5%, 15% ACN to 0% NH4HCO3 0.5%, 100% ACN). The fractions containing the product were mixed and concentrated to afford an intermediate fraction (10 mg) which was freeze-dried with acetonitrile/water 20/80 to give 9 mg (4%) of compound 9 as a yellow powder. MP: gum at 80° C. (K).
Analytical Part
LCMS (Liquid Chromatography/Mass Spectrometry) (See Table A1)
The LC measurement was performed using a UPLC (Ultra Performance Liquid Chromatography) Acquity (Waters) system comprising a binary pump with degasser, an autosampler, a diode-array detector (DAD) and a column as specified in the respective methods below, the column is hold at a temperature of 40° C. Flow from the column was brought to a MS detector. The MS detector was configured with an electrospray ionization source. The capillary needle voltage was 3 kV and the source temperature was maintained at 130° C. on the Quattro (triple quadrupole mass spectrometer from Waters). Nitrogen was used as the nebulizer gas. Data acquisition was performed with a Waters-Micromass MassLynx-Openlynx data system. Reversed phase UPLC was carried out on a Waters Acquity BEH (bridged ethylsiloxane/silica hybrid) C18 column (1.7 μm, 2.1×100 mm) with a flow rate of 0.343 ml/min. Two mobile phases (mobile phase A: 95% 7 mM ammonium acetate/5% acetonitrile; mobile phase B: 100% acetonitrile) were employed to run a gradient condition from 84.2% A and 15.8% B (hold for 0.49 minutes) to 10.5% A and 89.5% B in 2.18 minutes, hold for 1.94 min and back to the initial conditions in 0.73 min, hold for 0.73 minutes. An injection volume of 2 μl was used. Cone voltage was 20V for positive and negative ionization mode. Mass spectra were acquired by scanning from 100 to 1000 in 0.2 seconds using an interscan delay of 0.1 seconds.
DSC
For a number of compounds, melting points (MP) were determined with a DSC1 (Mettler-Toledo). Melting points were measured with a temperature gradient of 10° C./minute. Maximum temperature was 350° C. Values are peak values.
For a number of compounds, melting points were obtained with a Kofler hot bench, consisting of a heated plate with linear temperature gradient, a sliding pointer and a temperature scale in degrees Celsius.
NMR
For compound 1, 2, 6 to 9, the NMR experiments were carried out using a Bruker Avance III 500 using internal deuterium lock and equipped with an inverse triple-resonance (1H, 13C, 15N TXI) probe head. Chemical shifts (δ) are reported in parts per million (ppm).
For compound 3 and 4, each fraction was dissolved in 250 μl of water-free DMSO-d6 and the resulting solution was transferred into a 5 mm Shigemi NMR tube with a magnetic susceptibility matched for the respective solvent.
Experiments were recorded on a Bruker Avance 600 MHz spectrometer equipped with a inverse detection 5-mm cryoprobe (CPTCI). 1D 1H and 2D NOESY, HSQC and HMBC spectra were recorded running standard Bruker pulse programs. The NOESY spectrum was used for the determination of through-space connectivities; the HMBC spectrum for through-bond connectivities. Chemical shifts (δ) are reported in ppm. 1H NMR chemical shift data were obtained from the 1D 1H spectrum using the centre of the DMSO-d5 multiplet at 2.50 ppm or the centre of the acetonitrile-d2 multiplet at 1.94 ppm as internal reference. Coupling constants are measured in Hz. 13C NMR chemical shifts were obtained using the centre of the DMSO-d6 multiplet at 39.51 ppm as internal reference.







TABLE A1







Co. No. means compound number; Retention time (Rt) in minutes; MP means melting point (° C.).


As understood by a person skilled in the art, compounds synthesized using the protocols as


indicated may exist as a solvate e.g. hydrate, and/or contain residual solvent or minor impurities.












Co.


(Kofler(K)




No.
Compound
MP
or DSC)
Rt
[M + H]+





1




190° C.
DSC
2.46 (98.1% purity)
459


 


2




 80° C. (gummy)
K
2.29 (94.3% purity)
445


 


3




255° C.
K
2.07 (100% purity)
445


 


4




256° C.
K
2.06 (100% of purity)
445


 


5










 


6




170° C.
K
2.47 (99% of purity)
445


 


7




152° C. (gummy)
K
2.64 (95% of purity)
473



as HCl salt






 


8




266° C.
K
2.58 (95% of purity)
477


 


9




 80° C. (gummy)
K
2.23 (100% of purity)
473









Compound 1
1H NMR was performed at 350° K
1H NMR (500 MHz, DMSO-d6) δ ppm 0.99 (d, J=6.5 Hz, 6H) 2.84 (spt, J=6.5 Hz, 1H) 2.88-2.93 (m, 2H) 3.56 (br. s., 2H) 3.76 (s, 3H) 3.82-3.91 (m, 5H) 3.93 (s, 3H) 6.42 (d, J=2.2 Hz, 1H) 6.57 (d, J=2.2 Hz, 1H) 6.97 (d, J=2.7 Hz, 1H) 7.26 (dd, J=9.1, 2.7 Hz, 1H) 7.75 (d, J=9.1 Hz, 1H) 8.14 (s, 1H) 8.46 (s, 1H) 8.87 (s, 1H)
Compound 2
1H NMR was performed at 350° K
1H NMR (500 MHz, DMSO-d6) δ ppm 0.99 (d, J=6.6 Hz, 6H) 2.84 (spt, J=6.6 Hz, 1H) 2.88-2.95 (m, 2H) 3.56 (br. s., 2H) 3.76 (s, 3H) 3.80-3.95 (m, 5H) 6.43 (d, J=2.2 Hz, 1H) 6.57 (d, J=2.2 Hz, 1H) 6.99 (d, J=2.7 Hz, 1H) 7.26 (dd, J=9.5, 2.7 Hz, 1H) 7.75 (d, J=9.5 Hz, 1H) 8.35 (br. s., 2H) 8.92 (s, 1H) 13.08 (br. s., 1H)
Compound 3
1H NMR was performed at 300° K
1H NMR (600 MHz, DMSO-d6) δ ppm 0.98 (d, J=6.42 Hz, 6H) 2.82 (spt, J=6.50 Hz, 1H) 2.88 (t, J=4.53 Hz, 2H) 3.69 (s, 3H) 3.91 (s, 3H) 6.29 (d, J=2.27 Hz, 1H) 6.42 (d, J=2.27 Hz, 1H) 6.89 (br. s., 1H) 7.25 (br. s., 1H) 7.75 (d, J=9.07 Hz, 1H) 8.18 (s, 1H) 8.53 (s, 1H) 8.89 (s, 1H)
Compound 4
1H NMR was performed at 300° K
1H NMR (600 MHz, DMSO-d6) δ ppm 0.96 (d, J=6.70 Hz, 6H) 2.81 (spt, J=6.70 Hz, 1H) 2.86 (t, J=4.34 Hz, 2H) 3.79 (s, 3H) 3.91 (s, 3H) 6.26 (d, J=1.89 Hz, 1H) 6.41 (d, J=2.27 Hz, 1H) 6.91 (br. s., 1H) 7.27 (br. s., 1H) 7.75 (d, J=9.44 Hz, 1H) 8.18 (s, 1H) 8.53 (s, 1H) 8.89 (s, 1H)
Compound 6
1H NMR was performed at 300° K
1H NMR (500 MHz, DMSO-d6) δ ppm 1.43 (t, J=7.3 Hz, 3H) 2.22 (br s, 3H) 2.82 (br s, 2H) 3.50-4.10 (m, 10H) 4.20 (q, J=7.3 Hz, 2H) 6.46 (d, J=1.9 Hz, 1H) 6.59 (d, J=1.9 Hz, 1H) 6.92 (br s, 1H) 7.25 (br d, J=7.3 Hz, 1H) 7.77 (d, J=9.1 Hz, 1H) 8.20 (s, 1H) 8.58 (s, 1H) 8.92 (s, 1H)
Compound 7
1H NMR was performed at 300° K
1H NMR (500 MHz, DMSO-d6) δ ppm 1.32 (dd, J=9.8, 6.6 Hz, 6H) 1.44 (t, J=7.4 Hz, 3H) 3.35-3.60 (m, 3H) 3.93 (s, 3H) 3.79 (s, 3H) 4.22 (q, J=7.5 Hz, 2H) 4.43 (br d, J=12.9 Hz, 1H) 4.58 (br s, 1H) 6.56 (d, J=2.5 Hz, 1H) 6.70 (d, J=2.2 Hz, 1H) 7.17 (br d, J=1.9 Hz, 1H) 7.30 (dd, J=9.3, 2.4 Hz, 1H) 7.84 (d, J=9.1 Hz, 1H) 8.23 (s, 1H) 8.62 (s, 1H) 9.02 (s, 1H) 10.26 (br s, 1H)
Compound 8
1H NMR was performed at 350° K
1H NMR (500 MHz, DMSO-d6) δ ppm 0.99 (d, J=6.6 Hz, 6H) 2.85 (spt, J=6.5 Hz, 1H) 2.92 (t, J=4.6 Hz, 2H) 3.58 (br s, 2H) 3.80-4.05 (m, 11H) 6.84 (d, J=6.9 Hz, 1H) 6.92 (br s, 1H) 7.20 (br d, J=9.5 Hz, 1H) 7.79 (d, J=9.1 Hz, 1H) 8.15 (s, 1H) 8.46 (s, 1H) 8.89 (s, 1H)
Compound 9
1H NMR was performed at 300° K
1H NMR (500 MHz, DMSO-d6) δ ppm 0.97 (br d, J=5.4 Hz, 6H) 1.64 (br s, 2H) 2.72 (br s, 2H) 2.84 (spt, J=6.4 Hz, 1H) 3.50-3.80 (m, 7H) 3.84 (s, 3H) 3.92 (s, 3H) 6.21 (d, J=2.2 Hz, 1H) 6.61 (d, J=2.5 Hz, 1H) 6.92 (br s, 2H) 7.71 (d, J=9.5 Hz, 1H) 8.19 (s, 1H) 8.54 (s, 1H) 8.91 (s, 1H)
Some signals of the diazepine ring are broadened beyond detection in spectra measured at 300 K in DMSO-d6.
Pharmacological Part
Biological Assays A
FGFR1 (Enzymatic Assay)
In a final reaction volume of 30 μL, FGFR1 (h) (25 ng/ml) was incubated with 50 mM HEPES pH 7.5, 6 mM MnCl2, 1 mM DTT, 0.1 mM Na3VO4, 0.01% Triton-X-100, 500 nM Btn-Flt3 and 5 μM ATP in the presence of compound (1% DMSO final). After incubation for 60 minutes at room temperature the reaction was stopped with 2.27 nM EU-anti P-Tyr, 7 mM EDTA, 31.25 nM SA-XL-665 and 0.02% BSA which was present for 60 minutes at room temperature. Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) signal (ex340 nm. Em 620 nm, em 655 nm) was measured afterwards and results are expressed in RFU (Relative Fluorescence Units). In this assay, the inhibitory effect of different compound concentrations (range 10 μM to 0.1 nM) was determined and used to calculate an IC50 (M) and pIC50 (−log IC50) value.
FGFR2 (Enzymatic Assay)
In a final reaction volume of 30 μL, FGFR2 (h) (150 ng/ml) was incubated with 50 mM HEPES pH 7.5, 6 mM MnCl2, 1 mM DTT, 0.1 mM Na3VO4, 0.01% Triton-X-100, 500 nM Btn-Flt3 and 0.4 μM ATP in the presence of compound (1% DMSO final). After incubation for 60 minutes at room temperature the reaction was stopped with 2.27 nM EU-anti P-Tyr, 7 mM EDTA, 31.25 nM SA-XL-665 and 0.02% BSA which was present for 60 minutes at room temperature. Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) signal (ex340 nm. Em 620 nm, em 655 nm) was measured afterwards and results are expressed in (Relative Fluorescence Units). In this assay, the inhibitory effect of different compound concentrations (range 10 μM to 0.1 nM) was determined and used to calculate an IC50 (M) and pIC50 (−log IC50) value.
FGFR3 (Enzymatic Assay)
In a final reaction volume of 30 μL, FGFR3 (h) (40 ng/ml) was incubated with 50 mM HEPES pH 7.5, 6 mM MnCl2, 1 mM DTT, 0.1 mM Na3VO4, 0.01% Triton-X-100, 500 nM Btn-Flt3 and 25 μM ATP in the presence of compound (1% DMSO final). After incubation for 60 minutes at room temperature the reaction was stopped with 2.27 nM EU-anti P-Tyr, 7 mM EDTA, 31.25 nM SA-XL-665 and 0.02% BSA which was present for 60 minutes at room temperature. Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) signal (ex340 nm. Em 620 nm, em 655 nm) was measured afterwards and results are expressed in RFU (Relative Fluorescence Units). In this assay, the inhibitory effect of different compound concentrations (range 10 μM to 0.1 nM) was determined and used to calculate an IC50 (M) and pIC50 (−log IC50) value.
FGFR4 (Enzymatic Assay)
In a final reaction volume of 30 μL, FGFR4 (h) (60 ng/ml) was incubated with 50 mM HEPES pH 7.5, 6 mM MnCl2, 1 mM DTT, 0.1 mM Na3VO4, 0.01% Triton-X-100, 500 nM Btn-Flt3 and 5 μM ATP in the presence of compound (1% DMSO final). After incubation for 60 minutes at room temperature the reaction was stopped with 2.27 nM EU-anti P-Tyr, 7 mM EDTA, 31.25 nM SA-XL-665 and 0.02% BSA which was present for 60 minutes at room temperature. Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) signal (ex340 nm. Em 620 nm, em 655 nm) was measured afterwards and results are expressed in RFU (Relative Fluorescence Units). In this assay, the inhibitory effect of different compound concentrations (range 10 μM to 0.1 nM) was determined and used to calculate an IC50 (M) and pIC50 (−log IC50) value.
KDR (VEGFR2) (Enzymatic Assay)
In a final reaction volume of 30 μL, KDR (h) (150 ng/ml) was incubated with 50 mM HEPES pH 7.5, 6 mM MnCl2, 1 mM DTT, 0.1 mM Na3VO4, 0.01% Triton-X-100, 500 nM Btn-Flt3 and 3 μM ATP in the presence of compound (1% DMSO final). After incubation for 120 minutes at room temperature the reaction was stopped with 2.27 nM EU-anti P-Tyr, 7 mM EDTA, 31.25 nM SA-XL-665 and 0.02% BSA which was present for 60 minutes at room temperature. Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) signal (ex340 nm. Em 620 nm, em 655 nm) was measured afterwards and results are expressed in RFU (Relative Fluorescence Units). In this assay, the inhibitory effect of different compound concentrations (range 10 μM to 0.1 nM) was determined and used to calculate an IC50 (M) and pIC50 (−log IC50) value.
Ba/F3-FGFR1 (Minus IL3 or Plus IL3) (Cellular Proliferation Assay)
In a 384 well plate, 100 nl of compound dilution in DMSO was sprayed before adding 50 μl cell culture medium (phenol red free RPMI-1640, 10% FBS, 2 mM L-Glutamine and 50 μg/ml Gentamycin) containing 20000 cells per well of Ba/F3-FGFR1-transfected cells. Cells were put in an incubator at 37° C. and 5% CO2. After 24 hours, 10 μl of Alamar Blue solution (0.5 mM K3Fe(CN)6, 0.5 mM K4Fe(CN)6, 0.15 mM Resazurin and 100 mM Phosphate Buffer) was added to the wells, incubated for 4 hours at 37° C. and 5% CO2 before RFU's (Relative Fluorescence Units) (ex. 540 nm., em. 590 nm.) were measured in a flurorescence plate reader.
In this assay, the inhibitory effect of different compound concentrations (range 10 μM to 0.1 nM) was determined and used to calculate an IC50 (M) and pIC50 (−log IC50) value.
As a counterscreen the same experiment was performed in the presence of 10 ng/ml murine IL3.
Ba/F3-FGFR3 (Minus IL3 or Plus IL3) (Cellular Proliferation Assay)
In a 384 well plate, 100 nl of compound dilution in DMSO was sprayed before adding 50 μl cell culture medium (phenol red free RPMI-1640, 10% FBS, 2 mM L-Glutamine and 50 μg/ml Gentamycin) containing 20000 cells per well of Ba/F3-FGFR3-transfected cells. Cells were put in an incubator at 37° C. and 5% CO2. After 24 hours, 10 μl of Alamar Blue solution (0.5 mM K3Fe(CN)6, 0.5 mM K4Fe(CN)6, 0.15 mM Resazurin and 100 mM Phosphate Buffer) was added to the wells, incubated for 4 hours at 37° C. and 5% CO2 before RFU's (Relative Fluorescence Units) (ex. 540 nm., em. 590 nm.) were measured in a flurorescence plate reader.
In this assay, the inhibitory effect of different compound concentrations (range 10 μM to 0.1 nM) was determined and used to calculate an IC50 (M) and pIC50 (−log IC50) value.
As a counterscreen the same experiment was performed in the presence of 10 ng/ml murine IL3.
Ba/F3-KDR (Minus IL3 or Plus IL3) (Cellular Proliferation Assay)
In a 384 well plate, 100 nl of compound dilution in DMSO was sprayed before adding 50 μl cell culture medium (phenol red free RPMI-1640, 10% FBS, 2 mM L-Glutamine and 50 μg/ml Gentamycin) containing 20000 cells per well of Ba/F3-KDR-transfected cells. Cells were put in an incubator at 37° C. and 5% CO2. After 24 hours, 10 μl of Alamar Blue solution (0.5 mM K3Fe(CN)6, 0.5 mM K4Fe(CN)6, 0.15 mM Resazurin and 100 mM Phosphate Buffer) was added to the wells, incubated for 4 hours at 37° C. and 5% CO2 before RFU's (Relative Fluorescence Units) (ex. 540 nm., em. 590 nm.) were measured in a flurorescence plate reader.
In this assay, the inhibitory effect of different compound concentrations (range 10 μM to 0.1 nM) was determined and used to calculate an IC50 (M) and pIC50 (−log IC50) value.
As a counterscreen the same experiment was performed in the presence of 10 ng/ml murine IL3.
Ba/F3-FGFR4 (Cellular Proliferation Assay)
In a 384 well plate, 100 nl of compound dilution in DMSO was sprayed before adding 50 μl cell culture medium (phenol red free RPMI-1640, 10% FBS, 2 mM L-Glutamine and 50 μg/ml Gentamycin) containing 20000 cells per well of Ba/F3-FGFR4-transfected cells. Cells were put in an incubator at 37° C. and 5% CO2. After 24 hours, 10 μl of Alamar Blue solution (0.5 mM K3Fe(CN)6, 0.5 mM K4Fe(CN)6, 0.15 mM Resazurin and 100 mM Phosphate Buffer) was added to the wells, incubated for 4 hours at 37° C. and 5% CO2 before RFU's (Relative Fluorescence Units) (ex. 540 nm., em. 590 nm.) were measured in a flurorescence plate reader.
In this assay, the inhibitory effect of different compound concentrations (range 10 μM to 0.1 nM) was determined and used to calculate an IC50 (M) and pIC50 (−log IC50) value.
Data for the compounds of the invention in the above assays are provided in Table A2.



















TABLE A2











BAF3-
BAF3-
BAF3-
BAF3-
BAF3-
BAF3-









FGFR1
FGFR1
FGFR3
FGFR3
KDR
KDR








VEGFR
(MIN
(PLUS
(MIN
(PLUS
(MIN
(PLUS
BAF3-


Comp.
FGFR1
FGFR2
FGFR3
FGFR4
KDR
IL3
IL3
IL3
IL3
IL3
IL3
FGFR4


No.
pIC50
pIC50
pIC50
pIC50
pIC50
pIC50)
pIC50)
pIC50)
pIC50)
pIC50)
pIC50)
(pIC50)







1
6.3
6.5
6.1
5.3
5.6
5.2
<5
~5.0
<5
<5
<5
5.0









Biological Assays B
Enzyme Binding Assays (KINOMEscan®)
Kinase enzyme binding affinities of compounds disclosed herein were determined using the KINOMEscan® technology performed by DiscoveRx Corporation, San Diego, Calif., USA (www.kinomescan.com). Table A3 reports the obtained Kd values (nM), with the Kd being the inhibitor binding constant:












TABLE A3






Kd FGFR1
Kd FGFR2
Kd FGFR3
Kd FGFR4
Kd VEGFR2


Compound
(nM)
(nM)
(nM)
(nM)
(nM)




















1
314
674
325
778
>3010


2
17
63
68
68
741


3
720
620
300
1900
>3000


4
740
900
870
>3000
>3000


6
2400
2900
2200
>3000
>3000


7
79
340
170
230
1700


9
54
117
138
355
922

## Claims

1. A compound selected from the group consisting of a compound of formula (I):



a tautomeric form, and stereochemically isomeric form thereof, wherein
n represents an integer equal to 1 or 2;
R1 represents hydrogen, C1-6alkyl, hydroxyC1-6alkyl, C1-6alkyl substituted with —C(═O)NHCH3, or C1-6alkyl substituted with —S(═O)2— C1-4alkyl;
R2a represents hydrogen, fluoro or chloro;
R2b or R2c each independently represent methoxy or hydroxyl;
R3 represent hydrogen, C1-6alkyl, C3-6cycloalkyl, or C1-2alkyl substituted with C3-6cycloalkyl;
R4 represents hydrogen, methyl or ethyl;
or a pharmaceutically acceptable salt thereof or a solvate thereof.

2. A compound according to claim 1, wherein the compound has the following structure



or a pharmaceutically acceptable salt thereof or a solvate thereof.

3. A compound according to claim 1 wherein R2a represents hydrogen or fluoro, or a pharmaceutically acceptable salt thereof or a solvate thereof.

4. A compound according to claim 3 wherein R2a represents fluoro, or a pharmaceutically acceptable salt thereof or a solvate thereof.

5. A compound according to claim 1, wherein the compound has the following structure



or a pharmaceutically acceptable salt thereof or a solvate thereof.

6. A compound according to claim 1 wherein n represents an integer equal to 1, or a pharmaceutically acceptable salt thereof or a solvate thereof.

7. A compound according to claim 1 wherein R3 represents hydrogen, or a pharmaceutically acceptable salt thereof or a solvate thereof.

8. A compound according to claim 1 wherein R3 represents C1-6alkyl, in particular C1-4alkyl, or a pharmaceutically acceptable salt thereof or a solvate thereof.

9. A compound according to claim 1, wherein the compound has the following structure



or a pharmaceutically acceptable salt thereof or a solvate thereof.

10. A compound according to claim 1 wherein R1 represents hydrogen or C1-6alkyl, or a pharmaceutically acceptable salt thereof or a solvate thereof.

11. A compound according to claim 10 wherein R1 represents C1-6alkyl, or a pharmaceutically acceptable salt thereof or a solvate thereof.

12. A compound according to claim 11 wherein R1 represents methyl, or a pharmaceutically acceptable salt thereof or a solvate thereof.

13. A compound according to claim 1 wherein R2b represents methoxy, or a pharmaceutically acceptable salt thereof or a solvate thereof.

14. A compound according to claim 1 wherein R2b represents hydroxy, or a pharmaceutically acceptable salt thereof or a solvate thereof.

15. A compound according to claim 1 wherein R2c represents methoxy, or a pharmaceutically acceptable salt thereof or a solvate thereof.

16. A compound according to claim 1 wherein R2c represents hydroxy, or a pharmaceutically acceptable salt thereof or a solvate thereof.

17. The compound



or a pharmaceutically acceptable salt or solvate thereof.

18. A compound according to claim 17 wherein the compound is

19. A compound selected from



or a pharmaceutically acceptable salt or solvate thereof.

20. A process for the preparation of a compound of formula (I) as claimed in claim 1, or a pharmaceutically acceptable salt thereof or a solvate thereof, which process comprises:
reacting a compound of formula (II)




with formaldehyde in the presence of a suitable solvent, at a suitable temperature,
wherein R1, R2a, R2b, R2c, R3, R4 and n are as defined in claim 1; and optionally thereafter converting one compound of the formula (I) into another compound of the formula (I).

21. A pharmaceutical composition comprising a compound as defined in claim 1, or a pharmaceutically acceptable salt thereof or a solvate thereof and a pharmaceutically acceptable carrier.

22. A pharmaceutical composition comprising a compound as defined in claim 18, and a pharmaceutically acceptable carrier.